gebhart/patrepeval huggingface dataset:
Supplementary Figure 2 from HEF1 Is a Crucial Mediator of the Proliferative Effects of Prostaglandin E<sub>2</sub> on Colon Cancer Cells (2023)

Supplementary Figure 2 from HEF1 Is a Crucial Mediator of the Proliferative Effects of Prostaglandin E&lt;sub&gt;2&lt;/sub&gt; on Colon Cancer Cells
Dual-Source Swept-Source Optical Coherence Tomography Reconstructed on Integrated Spectrum (2011)

Dual-source swept-source optical coherence tomography (DS-SSOCT) has two individual sources with different central wavelengths, linewidth, and bandwidths. Because of the difference between the two sources, the individually reconstructed tomograms from each source have different aspect ratio, which makes the comparison and integration difficult. We report a method to merge two sets of DS-SSOCT raw data in a common spectrum, on which both data have the same spectrum density and a correct separation. The reconstructed tomographic image can seamlessly integrate the two bands of OCT data together. The final image has higher axial resolution and richer spectroscopic information than any of the individually reconstructed tomography image.
Ruthenium-catalyzed aldol and Michael reactions of activated nitriles (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRuthenium-catalyzed aldol and Michael reactions of activated nitrilesTakeshi Naota, Hiroshi Taki, Masahiko Mizuno, and Shunichi MurahashiCite this: J. Am. Chem. Soc. 1989, 111, 15, 5954–5955Publication Date (Print):July 1, 1989Publication History Published online1 May 2002Published inissue 1 July 1989https://pubs.acs.org/doi/10.1021/ja00197a073https://doi.org/10.1021/ja00197a073research-articleACS PublicationsRequest reuse permissionsArticle Views1091Altmetric-Citations121LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Bronchoalveolar Lavage Cell Gene Expression in Acute Lung Rejection: Development of a Diagnostic Classifier (2008)

There is a prominent acute rejection-associated signature in BAL cells characterized by increased T-cell, CD8 cytotoxic cell, and neutrophil gene expression. These findings lay the foundation for development of rapid PCR-based assays of gene expression for clinical acute rejection diagnosis.
Surface functionalized alumina nanoparticle filled polymeric nanocomposites with enhanced mechanical properties (2006)

Alumina nanoparticles were successfully functionalized with a bi-functional coupling agent, (3-methacryloxypropyl)trimethoxysilane (MPS), through a facile neutral solvent method. MPS was found to be covalently bound with the nanoparticles. The linked MPS was polymerized with a vinyl-ester resin monomer through a free radical polymerization. Atomic force microscope phase images showed a uniform distribution of nanoparticles. Microtensile test results revealed the Young's modulus and strength increasing with particle loading. Microscopic examinations revealed the presence of large plastic deformations at the micron scale in the nanocomposites in agreement with the observed strengthening effect of functionalized nanoparticles. Thermo-gravimetric analysis (TGA) did not show any significant change in the thermal degradation of the nanocomposite as compared with the neat resin. The polymer matrix effectively protected the alumina nanoparticles from dissolution in basic and acidic solutions.
Cloning and sequence analysis of the gene for a carbapenem-hydrolyzing class A beta-lactamase, Sme-1, from Serratia marcescens S6 (1994)

Serratia marcescens S6 produces a pI 9.7 carbapenem-hydrolyzing beta-lactamase that is probably encoded by the chromosome (Y. Yang, P. Wu, and D. M. Livermore, Antimicrob. Agents Chemother. 34:755-758, 1990). A total of 11.3 kb of genomic DNA from this strain was cloned into plasmid pACYC184 in Escherichia coli. After further subclonings, the carbapenem-hydrolyzing beta-lactamase gene (blaSme-1) was sequenced (EMBL accession number Z28968). The gene corresponded to an 882-bp open reading frame which encoded a 294-amino-acid polypeptide. This open reading frame was preceded by a -10 and a -35 region consistent with a putative promoter sequence of members of the family Enterobacteriaceae. This promoter was active in E. coli and S. marcescens, as demonstrated by primer extension analysis. N-terminal sequencing showed that the Sme-1 enzyme had a 27-amino-acid leader peptide and enabled calculation of the molecular mass of the mature protein (29.3 kDa). Sequence alignment revealed that Sme-1 is a class A serine beta-lactamase and not a class B metalloenzyme. The earlier view that the enzyme was zinc dependent was discounted. Among class A beta-lactamases, Sme-1 had the greatest amino acid identity (70%) with the pI 6.9 carbapenem-hydrolyzing beta-lactamase, NMC-A, from Enterobacter cloacae NOR-1. Comparison of these two protein sequences suggested a role for specific residues in carbapenem hydrolysis. The relatedness of Sme-1 to other class A beta-lactamases such as the TEM and SHV types was remote. This work details the sequence of the second carbapenem-hydrolyzing class A beta-lactamase from an enterobacterial species and the first in the genus Serratia.
Detection of lysosomal cysteine proteinases in microglia: Flow cytometric measurement and histochemical localization of cathepsin B and L (1993)

The activation and differentiation of microglia is a prominent pathophysiological process in numerous inflammatory and demyelinating diseases of the central nervous system, including Alzheimer's disease and the AIDS encephalopathy. The tissue damage during these diseases has partly been attributed to lipid peroxidating reactive oxygen intermediates for which activated microglia are a major source. The destruction of tissue may also involve the release of proteolytic enzymes, such as the lysosomal cysteine proteinases cathepsin B and L, which are present notably in phagocytic cells. The cathepsins B and L are endopeptidases with a substrate specificity including important proteins, like myelin basic protein, extracellular matrix components, or the class II major histocompatibility complex. Because of this pathophysiological relevance the cathepsins B and L were chosen for histochemical demonstration in isolated and cultured rat microglia and measurement by a new flow cytometric method. Cathepsin B/L activity was measured flow cytometrically in single viable cells by the intracellular cleavage of non-fluorescent (Z-Phe-Arg)2-rhodamine 110 to the green fluorescent monoamide Z-Phe-Arg-rhodamine 110 and rhodamine 110. In microglia we measured a cathepsin B/L activity that was 2.5 times higher than in thioglycolate-elicited, i.e., inflammatory peritoneal rat macrophages. In elicited peritoneal macrophages the formation of fluorescent product was 6.2 times higher than in unstimulated resident peritoneal macrophages, demonstrating that the activation and differentiation of mononuclear phagocytes is accompanied by an increased cathepsin B/L enzyme activity. The subcellular localization of cathepsin B/L activity in plated viable microglia was demonstrated histochemically by the use of Z-Ala-Arg-Arg-4-methoxy-2-naphthylamide. Its blue fluorescent cleavage product 4-methoxy-2-naphthylamide was found in lysosomes. Our study shows that activated microglia are an important potential source of cathepsin B/L. This is particularly interesting as enzymatically active cathepsins have recently been found extracellularly at high levels in the senile plaques of Alzheimer's disease, which are known to contain many activated microglia. The release of proteinases by microglia may play a crucial role in the pathomechanism of tissue-destructing diseases in the brain.
Evaluation of F-18-FDG in various experimental tumor models in mice (2006)

1623 Objectives: PET using the glucose analogue F-18-FDG is widely used for detecting and staging malignant tumors, as well as for monitoring therapy response. These applications are based on the increased F-18-FDG uptake due to enhanced glucose utilization in most tumors. The availability of valid mouse models would be of great interest e.g. for response assessment of already established and novel therapeutic drugs with F-18-FDG. Therefore, we examined and compared the F-18-FDG uptake and kinetics in different mouse tumor models. Methods: The mouse tumors F9 (embryonal teratoma), C51 (colon carcinoma) and B16-F1 (melanoma) as well as the human tumors MaTu and MDA-MB 231 (breast carcinoma), A375 (melanoma), PC-3 and DU-145 (prostate carcinoma), U251 (glioblastoma), Capan-1 and PanC-1 (pancreas adenocarcinoma), A549 (NSCLC), SK-Mel3 (melanoma) and Caki-1 (clear cell renal carcinoma) were transplanted either subcutaneous or orthotopically in NMRI nu/nu mice. Approx. 3 µCi F-18-FDG were injected i.v. and an organ distribution study was performed at different time points until 2 h after injection. To analyze the effect of blood sugar on F-18-FDG-uptake, blood sugar level was adjusted with insulin or glucose prior to F-18-FDG application. Results: In most tumors, F-18-FDG accumulation reached a peak after 15 min and decreases very slowly afterwards. The highest accumulation of F-18-FDG of about 7 % ID/g was seen in B16-F1 tumors after 1 h, followed by F9 tumors with approx. 6 % ID/g. The other tumors ranged between 3 to 1 % ID/g. No difference could be observed, if a tumor was transplanted subcutaneously or orthotopically (PanC-1), or as allogeneic or xenogeneic transplants. An increase in blood sugar resulted in decreased uptake of F-18-FDG in brain, heart and also in the tumor. A decrease in blood sugar resulted in increased uptake of F-18-FDG in brain and heart, but a decrease in the tumor. Conclusions: Despite the widespread clinical use of F-18-FDG, the observed differences in tumor uptake in humans and mice are not fully understood. The predominant portion of tumors tested showed only low accumulation of F-18-FDG despite being fast growing or well perfused, with no effect of the blood sugar level on tumor F-18-FDG uptake. More studies have to be performed in order to fully understand the predictive power of mouse models for F-18-FDG imaging.
Portable Multiplexer Supporting Three Spatially Multiplexed Optical Channels (2016)

Design of the input end of a portable spatial multiplexer supporting three spatially multiplexed optical channels over a multimode fiber is presented. Screen projection of the output beam and its intensity profile is also reported.
Cell-specific metallothionein gene expression in mouse decidua and placentae (1989)

Oligodeoxyribonucleotide excess solution hybridization, Northern blot and in situ hybridization were used to analyze metallothionein gene expression in mouse decidua and placentae during gestation. Metallothionein (MT) -I and -II mRNA levels were constitutively elevated, 11- and 13-fold, respectively, relative to the adult liver, in the deciduum (D8), and decreased coordinately about 6-fold during the period of development when the deciduum is replaced by the developing placenta (D10-16). Coincident with this decline, levels of MT mRNA increased dramatically in the visceral yolk sac endoderm. In situ hybridization established that MT-I mRNA was present at low levels in the uterine luminal epithelium (D4), but was elevated at the site of embryo implantation exclusively in the primary decidual zone by D5, and then in the secondary decidual zone (D6-8). Although low levels of MT mRNA were detected in total placental RNA, in situ hybridization revealed constitutively high levels in the outer placental spongiotrophoblasts. Analysis of pulse-labeled proteins from decidua and placentae established that these tissues are active in the synthesis of MT. The constitutively high levels of MT mRNA in decidua were only slightly elevated following injection of cadmium (Cd) and/or zinc (Zn), whereas in placentae they increased several-fold. MT mRNA levels were equally high in decidua and experimentally induced deciduomata (D8) which establishes that decidual MT gene expression is not dependent on the presence of the embryo or some embryo-derived factor. Although the functional role of MT during development is speculative, these results establish the concept that, from the time of implantation to late in gestation, the mouse embryo is surrounded by cells, interposed between the maternal and embryonic environments, which actively express the MT genes. This suggests that MT plays an important role in the establishment and maintenance of normal pregnancy.
Dynamics of Large-Scale Structures for Jets in a Crossflow (1998)

Results of a three dimensional unsteady computational study of a row of jets injected normal to a cross-flow are presented with the aim of understanding the dynamics of the large scale structures in the region near the jet. The jet to cross-flow velocity ratio is .5. A modified version of the computer program (INS3D) which utilizes the method of artificial compressibility is used for the computations. Results obtained clearly indicate that the near field large scale structures are extremely dynamical in nature, and undergo breakup and reconnection processes. The dynamical near field structures identified include the counter rotating vortex pair (CVP), the horseshoe vortex, wake vortex, wall vortex and the shear layer vortex. The dynamical features of these vortices are presented in this paper. The CVP is observed to be a convoluted structure interacting with the wall and horseshoe vortices. The shear layer vortices are stripped by the crossflow, and undergo pairing and stretching events in the leeward side of the jet. The wall vortex is reoriented into the upright wake system. Comparison of the predictions with mean velocity measurements is made. Reasonable agreement is observed.
Distributed clock gating for power reduction of a programmable waveform generator for neural stimulation (2012)

This paper describes how to employ distributed clock gating to achieve an overall low power design of a programmable waveform generator intended for a neural stimulator. The power efficiency is enabled using global timing control combined with local amplitude distribution over a bus to the local stimulator frontends. This allows the combination of local and global clock gating for complete sub-blocks of the design. A counter and a shifter employed at the local digital stimulator reduce the design complexity for the waveform generation and thus the overall power consumptions. The average power results indicate that 63% power can be saved for the global stimulator control unit and 89-96% power can be saved for the local digital stimulator by using the proposed approach. The circuit has been implemented and successfully tested in a 0.35 µm AMS HVCMOS technology.
A “green” technique for high performance elastomers – fundamental investigation for hydrogenation of nitrile butadiene rubber in supercritical carbon dioxide (2002)

NBR hydrogenation, catalyzed by OsHCl(CO)(O2)(PCy3)2 is investigated in supercritical carbon dioxide (SC-CO2). The experiments were carried out in a volume-changeable SFT Phase Monitor with a view window. It was found that the catalyst could maintain steady activity in SC-CO2 for a long time and no deactivation or decomposition of the catalyst was observed during the experimental operation periods. The reaction reached a conversion of 98% within 2.5 hours at 150°C. Increasing the temperature, specific area of NBR, CO2 density and catalyst concentration favours higher hydrogenation degree, but the effect of hydrogen pressure is complicated and attention must be given to find the optimal hydrogen pressure. The results suggest that NBR hydrogenation could be possibly realized in SCFs in place of conventional organic solvents.
PARITY AND DEATH FROM COLON CANCER IN WOMEN: A CASE‐CONTROL STUDY (1984)

Abstract Within the population of ever‐married women, aged 40 or over, in South Australia, the parity distributions of 515 consecutive registered deaths attributed to colon cancer during 1976–1980, 17,941 consecutive deaths from other causes, and the mid‐1976 census population of 203,718 women were compared. The age‐adjusted relative risks of colon cancer death, using the census population as controls and treating nulliparous women as RR = 1.00, were 0.72 and 0.63 for para 1–2 and para 3‐plus women respectively. These risk reductions were statistically significant (p&lt;0.05). Comparison of cases with other deceased women as controls yielded lesser reductions in risk associated with increasing parity (RR = 0.89 and 0.80); the equivalent comparison for ever‐married men showed a negligible change in risk associated with having fathered children (RR = 0.98). The similarity of these findings with other recent reports of colon cancer in relation to parity in women, and their likely biological significance, is discussed.
Towards personalized smart city guide services in future internet environments (2015)

The FI-CONTENT project aims at establishing the foundation of a European infrastructure for developing and testing novel smart city services. The Smart City Services Platform will develop enabling technology for SMEs and developer to create services offering residents and visitors to cities smart services that enhance their city visit or daily life. We have made use of generic, specific and common enablers to develop a reference implementation, the Smart City Guide web app. The basic information is provided by the Open City Database, an open source specific enabler that can be used for any city in Europe. Recommendation as a Service is an enabler that can be applied to lots use cases, here we describe how we integrated it into the Smart City Guide. The uses cases will be iteratively improved and upgraded during regular iterative cycles based on feedback gained in lab and field trials at the experimentation sites. As the app is transferable to any city, it will be tested at a number of experimentation sites.
Code Generation and Optimization for Transactional Memory Constructs in an Unmanaged Language (2007)

Transactional memory offers significant advantages for concurrency control compared to locks. This paper presents the design and implementation of transactional memory constructs in an unmanaged language. Unmanaged languages pose a unique set of challenges to transactional memory constructs - for example, lack of type and memory safety, use of function pointers, aliasing of local variables, and others. This paper describes novel compiler and runtime mechanisms that address these challenges and optimize the performance of transactions in an unmanaged environment. We have implemented these mechanisms in a production-quality C compiler and a high-performance software transactional memory runtime. We measure the effectiveness of these optimizations and compare the performance of lock-based versus transaction-based programming on a set of concurrent data structures and the SPLASH-2 benchmark suite. On a 16 processor SMP system, the transaction-based version of the SPLASH-2 benchmarks scales much better than the coarse-grain locking version and performs comparably to the fine-grain locking version. Compiler optimizations significantly reduce the overheads of transactional memory so that, on a single thread, the transaction-based version incurs only about 6.4% overhead compared to the lock-based version for the SPLASH-2 benchmark suite. Thus, our system is the first to demonstrate that transactions integrate well with an unmanaged language, and can perform as well as fine-grain locking while providing the programming ease of coarse-grain locking even on an unmanaged environment
Effects of otitis media on child development. (1993)

Otitis media is one of the most common diseases of childhood, and the broad continuum of this disease ranges from asymptomatic middle ear effusion to recurring or persistent infection. In spite of many modalities of successful treatment, there remains a large number of children with chronic otitis media after antibiotic management and even in some persistent cases after surgical management with ventilation and tube insertion. This report attempts to concisely review the studies on the effect of otitis media on childhood development.
Effect of lactic acid bacteria probiotic culture for the treatment of Salmonella enterica serovar Heidelberg in neonatal broiler chickens and turkey poults (2011)

In the present study, a series of experiments was conducted to evaluate the ability of a commercial probiotic culture (FloraMax, IVS-Wynco LLC, Springdale, AR) to reduce Salmonella enterica serovar Heidelberg (SH) in chicks and turkey poults. In experiments 1 and 2, chicks were randomly assigned to treatment groups and then challenged via oral gavage with SH. Chicks were treated 1 h following SH challenge with the probiotic culture via oral gavage. At 24 and 72 h posttreatment, cecal tonsils and ceca were collected for recovery and enumeration of enteric Salmonella Heidelberg, respectively. In experiment 3, day-of-hatch turkeys were randomly assigned to treatment groups and then challenged via oral gavage with SH. Poults were treated 1 h following challenge with the probiotic via oral gavage. At 24 and 72 h post probiotic treatment, cecal tonsils and ceca were collected for recovery and enumeration of enteric SH, respectively. The probiotic culture significantly reduced the incidence of SH in cecal tonsils at both time points in chicks in both experiments (P < 0.05). These data demonstrate that administration of probiotic 1 h post SH challenge significantly reduced the incidence of SH recovery from cecal tonsils of chicks compared with controls 24 and 72 h following treatment. Similarly, probiotic treatment resulted in significant reductions in the concentrations of SH within the ceca in both experiments. Although similar significant results were observed at both 24 and 72 h in experiment 3, it was clear that poults were more susceptible to SH colonization than chicks. Overall, a Lactobacillus-based probiotic significantly reduced Salmonella enterica serovar Heidelberg in chicks and turkey poults.
Measuring Information Transfer (2000)

An information theoretic measure is derived that quantifies the statistical coherence between systems evolving in time. The standard time delayed mutual information fails to distinguish information that is actually exchanged from shared information due to common history and input signals. In our new approach, these influences are excluded by appropriate conditioning of transition probabilities. The resulting transfer entropy is able to distinguish effectively driving and responding elements and to detect asymmetry in the interaction of subsystems.