gebhart/patrepeval huggingface dataset:
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization (2015)

The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible applications in controlling cell morphology/orientation. Nanofiber organization is controlled with a new fabrication apparatus that enables the gradual decrease of fiber organization in a scaffold. Changing the alignment of fibers is achieved through decreasing deposition time of random electrospun fibers on a uniaxially aligned fiber mat. By covering the collector with a moving barrier/mask, along the same axis as fiber deposition, the organizational structure is easily controlled. For tissue engineering purposes, adipose-derived stem cells can be seeded to these scaffolds. Stem cells undergo morphological changes as a result of their position on the varied organizational structure, and can potentially differentiate into different cell types depending on their locations. Additionally, the graded organization of fibers enhances the biomimicry of nanofiber scaffolds so they more closely resemble the natural orientations of collagen nanofibers at tendon-to-bone insertion site compared to traditional scaffolds. Through nanoencapsulation, the gradated fibers also afford the possibility to construct chemical gradients in fiber scaffolds, and thereby further strengthen their potential applications in fast screening of cell-materials interaction and interfacial tissue regeneration. This technique enables the production of continuous gradient scaffolds, but it also can potentially produce fibers in discrete steps by controlling the movement of the moving barrier/mask in a discrete fashion.
Reduction of the cogging in the linear synchronous motor with a claw-pole-structured mover (2008)

This paper describes novel claw-pole movers interior permanent magnets linear synchronous motor (CPIP-LSM). However the cogging of the CPIP-LSM was very large, it was almost 100% of maximum thrust in the initial model. Changing parameters of the CPIP-LSM partially at 1-phase-driven, and using 3-phase-driven the cogging was dramatically decreased. Finally it was confirmed with the high-thrust model, that the cogging of the CPIP-LSM is very small.
Interdisciplinarity and innovation dynamics. On convergence of research, technology, economy, and society (2011)

In the age of globalization, economic growth and the welfare of nations decisively depend on basic innovations. Therefore, education and knowledge is an important advantage of competition in highly developed countries with high standards of salaries, but raw material shortage. In the twenty-first century, innovations will arise from problem-oriented research, crossing over traditional faculties and disciplines. Therefore, we need platforms of interdisciplinary dialogue to choose transdisciplinary problems (e.g., environment, energy, information, health, welfare) and to cluster new portfolios of technologies. The clusters of research during the excellence initiative at German universities are examples of converging sciences. The integration of natural and engineering sciences as well as medicine can only be realized if the research training programs (e.g., graduate schools) generate a considerable added value in terms of multidisciplinary experience, international networking, scientific and entrepreneurial know-how, and personality development. The Carl von Linde-Academy is presented as an example of an interdisciplinary center of research and teaching at the Technical University of Munich.
Is Polymethyl Methacrylate a Viable Option for Salvaging Lateral Mass Screw Failure in the Subaxial Cervical Spine? (2014)

Study Design Biomechanical analysis of lateral mass screw pullout strength. Objective We compare the pullout strength of our bone cement-revised lateral mass screw with the standard lateral mass screw. Methods In cadaveric cervical spines, we simulated lateral mass screw "cutouts" unilaterally from C3 to C7. We salvaged fixation in the cutout side with polymethyl methacrylate (PMMA) or Cortoss cement (Orthovita, Malvern, Pennsylvania, United States), allowed the cement to harden, and then drilled and placed lateral mass screws back into the cement-augmented lateral masses. On the contralateral side, we placed standard lateral mass screws into the native, or normal lateral, masses and then compared pullout strength of the cement-augmented side to the standard lateral mass screw. For pullout testing, each augmentation group was fixed to a servohydraulic load frame and a specially designed pullout fixture was attached to each lateral mass screw head. Results Quick-mix PMMA-salvaged lateral mass screws required greater force to fail when compared with native lateral mass screws. Cortoss cement and PMMA standard-mix cement-augmented screws demonstrated less strength of fixation when compared with control-side lateral mass screws. Attempts at a second round of cement salvage of the same lateral masses led to more variations in load to failure, but quick-mix PMMA again demonstrated greater load to failure when compared with the nonaugmented control lateral mass screws. Conclusion Quick-mix PMMA cement revision equips the spinal surgeon with a much needed salvage option for a failed lateral mass screw in the subaxial cervical spine.
Conversion of 1-O-[3H]alkyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine to lyso platelet-activating factor by the CoA-independent transacylase in membrane fractions of human neutrophils. (1991)

The first step in the synthesis of platelet-activating factor (PAF) in stimulated neutrophils is generally accepted to be hydrolysis of 1-O-alkyl-2-acyl-sn-glycero-3-phosphorylcholine (1-O-alkyl-2-acyl-GPC), with 1-O-alkyl-2-arachidonoyl-GPC being the preferred precursor. Characterization of the enzymatic activity responsible for the hydrolysis of 1-O-alkyl-2-arachidonoyl-GPC has been hampered by lack of an active and reliable cell-free system for study. In the present studies, membrane preparations containing 1-O-[3H]alkyl-2-arachidonoyl-GPC were prepared from intact human neutrophils that had been labeled using 1-O-[3H]hexadecyl-2-lyso-GPC. When the labeled membrane preparations were incubated in the presence of unlabeled 1-O-alkyl-2-lyso-GPC (5 microM), rapid deacylation (up to 25% of the label in 10 min) of the 1-O-[3H]alkyl-2-arachidonoyl-GPC to 1-O-[3H]alkyl-2-lyso-GPC (lyso-PAF) was observed. The deacylation activity appeared to be the same in preparations from resting or stimulated cells. No requirement for Ca2+, various nucleotides, or protein kinase activation could be demonstrated. A number of observations indicated that [3H]lyso-PAF is formed in the system by the action of the CoA-independent transacylase present in the cells rather than by phospholipase A2. Both 1-O-alkyl-2-lyso-GPC and 1-acyl-2-lyso-GPC elicited deacylation of 1-O-[3H]alkyl-2-arachidonoyl-GPC, whereas neither 3-O-alkyl-2-lyso-GPC nor 1-O-alkyl-2-O-methyl-rac-glycero-3-phosphorylcholine, which should act as detergents but are not transacylase substrates, effected deacylation. The deacylation activity and CoA-independent transacylase activities were blocked in parallel by a number of inhibitors and by heat inactivation. In preparations containing 1-O-alkyl-2-[3H]arachidonoyl-GPC, no release of free [3H]arachidonic acid was observed. However, a shift of the [3H]arachidonate into exogenous 1-O-tetradecyl-2-lyso-GPC was observed in the system. These findings are consistent with the generation of [3H]lyso-PAF by the CoA-independent transacylase activity.
Nuclear Magnetic Relaxation Study of Intermolecular Complexes. The Mechanism of Penicillin Binding to Serum Albumin1a (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear Magnetic Relaxation Study of Intermolecular Complexes. The Mechanism of Penicillin Binding to Serum Albumin1aJames J. Fischer and Oleg JardetzkyCite this: J. Am. Chem. Soc. 1965, 87, 14, 3237–3244Publication Date (Print):July 1, 1965Publication History Published online1 May 2002Published inissue 1 July 1965https://pubs.acs.org/doi/10.1021/ja01092a040https://doi.org/10.1021/ja01092a040research-articleACS PublicationsRequest reuse permissionsArticle Views216Altmetric-Citations134LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Oct4 Is Crucial for Transdifferentiation of Hepatocytes to Biliary Epithelial Cells in an In Vitro Organoid Culture Model (2017)

Hepatocyte to biliary transdifferentiation has been documented in various models of bile duct injury. In this process, mature hepatocytes transform into mature biliary epithelial cells by acquiring biliary phenotypic markers. Several signaling pathways including PI3 kinase, Notch, Hes1, Sox9, and Hippo are shown to be involved in the process. However, whether Oct4 is involved in hepatocyte to biliary transdifferentiation is unknown. We investigated the role of Oct4 in hepatocyte to biliary transdifferentiation utilizing an in vitro organoid culture system as a model of transdifferentiation. Oct4 was inhibited using adenovirus containing Oct4 shRNA. Hepatocyte-specific HNF-4α and biliary-specific HNF-1β and CK19 expression were assessed to gauge the extent of transdifferentiation. Oct4 was induced during hepatocyte to biliary transdifferentiation. Oct4 inhibition significantly downregulated the appearance of biliary cells from hepatocytes. This was accompanied by a significant downregulation of signaling pathways including Notch, Sox9, and Hippo. Our findings suggest that Oct4 is crucial for hepatocyte to biliary transdifferentiation and maturation and that it acts upstream of Notch, Sox9, and Hippo signaling in this model. This finding identifies new signaling through Oct4 in plasticity between hepatocytes and biliary epithelial cells, which can be potentially utilized to identify new strategies in chronic biliary diseases.
A Source Book of Australian Legal History: Source Materials from the Eighteenth to the Twentieth Centuries (1981)

Journal Article A Source Book of Australian Legal History: Source Materials from the Eighteenth to the Twentieth Centuries Get access Bennett J. M. Castles Alex C., Editors, A Source Book of Australian Legal History: Source Materials from the Eighteenth to the Twentieth Centuries. Law Book Co. Ltd., 1979. x, 299 pp. $24.50. James A. Thomson James A. Thomson Barrister and Solicitor High Court of Australia Search for other works by this author on: Oxford Academic Google Scholar American Journal of Legal History, Volume 25, Issue 2, April 1981, Pages 173–175, https://doi.org/10.2307/844637 Published: 01 April 1981
Supplementary Figure Legend from EGFR Exon 18 Mutations in Lung Cancer: Molecular Predictors of Augmented Sensitivity to Afatinib or Neratinib as Compared with First- or Third-Generation TKIs (2023)

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Broadband RF Noise Suppression by Magnetic Nanowire-Filled Composite Films (2009)

The characteristics of the electromagnetic noise suppression have evaluated using magnetic nanowire-filled composite film on a microstrip line in the broadband radio-frequency range from 0.5 to 20 GHz. The ferromagnetic resonance frequency and the relative complex permeability with the change of aspect ratio were calculated by Kittel's and Landau-Lifshitz-Gilbert equations. The FMR frequency is gradually increased with the increment of aspect ratio (up to 50) of magnetic nanowire. When the magnetic nanowires were mixed with various aspect ratio in composite, the FMR frequency exhibited the broadband frequency regions with 10-28 GHz, 5-14 GHz, 2.5-7 GHz, and 1-2.7 GHz for the change of magnetizations (4piM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">s</sub> : 20, 10, 5, and 2 kG), respectively. Using the calculated FMR frequency profile, the power losses were obtained in broadband frequency region. The transmission power absorption of the composite film on microstrip line was simulated using a 3-D FEM HFSS simulation program. The microstrip line model was composed of Cu conductor line and grounded dielectric substrates, which was designed by IEC standard (IEC 62333-2). The conduction electromagnetic noise was suppressed by the various aspect ratios of the magnetic nanowire-filled composite films in broadband frequency region.
Effect of Elemental Sulfur on Corrosion in Sour Gas Systems (1991)

The corrosive interaction of wet elemental sulfur with construction materials is reviewed, emphasizing effects of alloy composition (carbon steels, ferritic steels, austenitic steels, ferritic-austenitic [duplex] steels, Ni- and Co-base alloys, and titanium and its alloys), medium composition (salts, partial pressures of H2S and CO2, and acetic acid), and environmental conditions (temperature 20 to 250°C, stress). A summary of relevant physical and chemical properties of elemental sulfur is given to provide the fundamental basis for the understanding of sulfur corrosion. The different corrosion mechanisms found in the literature are critically discussed. Considering all data available, a mechanism is proposed explaining the electrochemical role of sulfur in the (physically) dissolved, solid, and liquid states. Methods for corrosion protection are outlined for carbon steels and corrosion-resistant alloys (CRA).
Comparison of polar metabolites in Australian deep-sea sponges using HPLC-ESI-HRMS in combination with multivariate analysis (2012)

More than 70% of the earth's surface is covered by oceans. Yet, of the 30,000 marine natural products reported, less than 2% derive from deep-sea organisms. There is a paucity of research on deep-sea natural products due to the prohibitively expensive nature and logistical difficulty of conducting deep-sea research. Using remotely operated vehicles, a variety of deep-sea sponges have been collected off the shelves of Australia at depths of 82–235m. These organisms were extracted with solvents of varying polarity and their polar extracts investigated using HPLC-ESI-HRMS in combination with principal components analysis. A total of 76 metabolites were identified from five deep-sea demosponges belonging to the genera Hyatella, Haliclona, Neopetrosia, Spongia and Pachastrella. These metabolites were used to determine similarities and differences between the different deep-sea species along with examining the influence of environmental parameters.. The outcome shows that sponges collected in similar locations produce similar polar metabolites, but at different geographic conditions such as location and depth, sponges produce different polar metabolites. Additionally, the isolation and characterisation of novel metabolites from the deep-sea sponge, Pachypellina sp. is also currently underway.
Universal Weighting Metric Learning for Cross-Modal Matching (2020)

Cross-modal matching has been a highlighted research topic in both vision and language areas. Learning appropriate mining strategy to sample and weight informative pairs is crucial for the cross-modal matching performance. However, most existing metric learning methods are developed for unimodal matching, which is unsuitable for cross-modal matching on multimodal data with heterogeneous features. To address this problem, we propose a simple and interpretable universal weighting framework for cross-modal matching, which provides a tool to analyze the interpretability of various loss functions. Furthermore, we introduce a new polynomial loss under the universal weighting framework, which defines a weight function for the positive and negative informative pairs respectively. Experimental results on two image-text matching benchmarks and two video-text matching benchmarks validate the efficacy of the proposed method.
Development of Antimicrobial Peptide–Antibiotic Conjugates to Improve the Outer Membrane Permeability of Antibiotics Against Gram-Negative Bacteria (2022)

Antibiotics have been widely used in the medical field as a treatment for infectious diseases, but they are not effective against all Gram-negative bacteria because of their low permeability to the outer membrane. One of the strategies to improve the antibacterial activity of antibiotics is the coadministration of antibiotics and membrane-perturbing antimicrobial peptides for their synergistic effects. However, because of their different pharmacokinetics, their coadministration may not exert expected effects in the clinical stage. Here, we designed various antimicrobial peptide-antibiotic conjugates as a novel approach to improve the antimicrobial activity of antibiotics. Ampicillin was chosen as a model antibiotic with poor outer membrane permeability, and the effects of the chemistry and position of conjugation and the choice of antimicrobial peptides were examined. One of the ampicillin conjugates exhibited significantly improved antimicrobial activity against ampicillin-resistant Gram-negative bacteria without exerting cytotoxicity against human cultured cells, demonstrating that our novel approach is an effective strategy to improve the antimicrobial activity of antibiotics with low outer membrane permeability.
Extended password key exchange protocols immune to dictionary attack (2002)

Strong password methods verify even small passwords over a network without additional stored keys or certificates with the user, and without fear of network dictionary attack. We describe a new extension to further limit exposure to theft of a stored password-verifier, and apply it to several protocols including the Simple Password Exponential Key Exchange (SPEKE). Alice proves knowledge of a password C to Bob, who has a stored verifier S, where S=g/sup C/ mod p. They perform a SPEKE exchange based on the shared secret S to derive ephemeral shared key K/sub 1/. Bob chooses a random X and sends g/sup X/ mod p. Alice computes K=g/sup XC/ mod p, and proves knowledge of {K/sub 1/,K/sub 2/}. Bob verifies this result to confirm that Alice knows C. Implementation issues are summarized, showing the potential for improved performance over Bellovin and Merritt's comparably strong Augmented-Encrypted Key Exchange. These methods make the password a strong independent factor in authentication, and are suitable for both Internet and intranet use.
Neurogranin in Alzheimer's disease and ageing: A human post-mortem study (2023)

Neurogranin (Ng), a post-synaptic protein involved in memory formation, has been investigated as a biomarker in the cerebrospinal fluid (CSF) in Alzheimer's disease (AD) and ageing. CSF Ng levels are elevated in AD relative to healthy controls and correlate with cognition; however, few studies have focused on Ng abundance in the brain. Synapse loss in the brain correlates closely with cognitive decline in AD making synaptic biomarkers potentially important for tracking disease progression, but the links between synaptic protein changes in CSF and brain remain incompletely understood. In the current study, Ng abundance was examined in post-mortem human brain tissue across AD, healthy ageing (HA), and mid-life (ML) cohorts. Ng levels were quantified in three brain regions associated with cognitive change found during ageing and neurodegenerative diseases, namely the middle temporal gyrus, primary visual cortex and the posterior hippocampus using immunohistochemistry. To support immunohistochemical analysis, total homogenate and biochemically enriched synaptic fractions from available temporal gyrus tissues were examined by immunoblot. Finally, we examined whether Ng is associated with lifetime cognitive ageing. Ng levels were significantly reduced in AD relative to HA and ML cases across all regions. Additionally Ng was significantly reduced in HA in comparison to ML in the primary visual cortex. Immunoblotting confirms reduced Ng levels in AD cases supporting immunohistochemical results. Interestingly, there was also a significant reduction of synapse-associated Ng in our group who had lifetime cognitive decline in comparison to the group with lifetime cognitive resilience indicating loss of neurogranin in remaining synapses during ageing is associated with cognitive decline. Our findings indicate that increases in CSF Ng reflect loss of brain neurogranin and support the use of CSF Ng as a biomarker of AD and potentially of cognitive decline in healthy ageing.
ChemInform Abstract: Synthesis, Structural Characterization, and Magnetic Properties of the Heteroleptic Dinuclear Nickel Selenite Complex [{Ni(TMEDA)SeO3}2]. (2011)

Abstract The structures of compounds (II) and (III) are determined by single crystal XRD.
Reversal of Vascular and Renal Crises of Scleroderma by Oral Angiotensin-Converting-Enzyme Blockade (1979)

UNTIL successful medical management of scleroderma crisis was reported,1 2 3 4 bilateral nephrectomy was thought to be the only therapy to control this emergency and permit survival.5 6 7 We report here two cases of dramatic reversal of scleroderma vascular and renal crises with the oral angiotensin-converting-enzyme inhibitor SQ 14225 (Captopril).8 , 9 Case Reports Case 1. Scleroderma was diagnosed in a 33-year-old woman in 1976. In 1977, a hospital evaluation revealed that her blood pressure was 120/80 mm Hg, serum creatinine 0.8 mg per 100 ml, creatinine clearance 86 ml per minute, urinary protein less than 100 mg per 24 hours, plasma renin activity 2.0 . . .
Magnetic Granulometry and Super-Paramagnetism (1956)

The magnetic technique of particle size measurement based on the work of Elmore consists of a comparison of a modified Langevin curve with the observed magnetization curve for an appropriate system of ferromagnetic particles. The average magnetic moment per particle is obtained from this analysis, and the average particle size is thus determined. The prediction by Néel of thermal relaxation in single-domain ferromagnetic particles broadens the range of applicability of the granulometry method, with special emphasis on the size range from 20 A to about 100 A. A possible objection to the method, arising from evidence for an abnormal temperature dependence of the saturation magnetization of very small magnetic particles, is re-examined experimentally and found to be invalid. Several examples are presented that demonstrate the predicted thermal relaxation and that point to a wide utilization of the granulometry technique.