gebhart/patrepeval huggingface dataset:
Effects of deoxygenation on active and passive Ca2+ transport and on the cytoplasmic Ca2+ levels of sickle cell anemia red cells. (1993)

Elevated [Ca2+]i in deoxygenated sickle cell anemia (SS) red cells (RBCs) could trigger a major dehydration pathway via the Ca(2+)-sensitive K+ channel. But apart from an increase in calcium permeability, the effects of deoxygenation on the Ca2+ metabolism of sickle cells have not been previously documented. With the application of 45Ca(2+)-tracer flux methods and the combined use of the ionophore A23187, Co2+ ions, and intracellular incorporation of the Ca2+ chelator benz-2, in density-fractionated SS RBCs, we show here for the first time that upon deoxygenation, the mean [Ca2+]i level of SS discocytes was significantly increased, two- to threefold, from a normal range of 9.4 to 11.4 nM in the oxygenated cells, to a range of 21.8 to 31.7 nM in the deoxygenated cells, closer to K+ channel activatory levels. Unlike normal RBCs, deoxygenated SS RBCs showed a two- to fourfold increase in pump-leak Ca2+ turnover. Deoxygenation of the SS RBCs reduced their Ca2+ pump Vmax, more so in reticulocyte- and discocyte-rich than in dense cell fractions, and decreased their cytoplasmic Ca2+ buffering. Analysis of these results suggests that both increased Ca2+ influx and reduced Ca2+ pump extrusion contribute to the [Ca2+]i elevation.
Maturation of Polycistronic Pre-mRNA in Trypanosoma brucei: Analysis of trans Splicing and Poly(A) Addition at Nascent RNA Transcripts from the hsp70 Locus (1991)

Numerous protein-coding genes of the protozoan Trypanosoma brucei are arranged in tandem arrays that are transcribed polycistronically. The pre-mRNA transcripts are processed by trans splicing, leading to the addition of a capped 39-nucleotide (nt) miniexon and by poly(A) addition. We wished to determine the order of the RNA processing events at the hsp70 locus and address the potential occurrence of cotranscriptional RNA processing. We determined the rate of transcriptional elongation at the hsp70 locus in isolated nuclei, which measured between 20 and 40 nt/min. This low rate of RNA chain elongation allowed us to label the 3' end of hsp70 nascent RNA with a short (about 180-nt) 32P tail. The structure of the labeled nascent hsp70 RNA could then be analyzed by RNase T1 and RNase T1/RNase A mapping. We show that the trans splicing of hsp70 pre-mRNA did not occur immediately after the synthesis of the 3' splice acceptor site, and nascent RNA molecules that contained about 550 nt of RNA beyond the 3' splice acceptor site still had not acquired a miniexon. In contrast, nascent RNA with a 5' end that mapped to the polyadenylation site of the hsp70 genes could be detected, indicating that maturation of the pre-mRNA in trypanosomes involves a rapid cleavage of the nascent hsp70 RNA (within seconds after synthesis of the site) for poly(A) addition. Our data suggest that polycistronic pre-mRNA is unlikely to be synthesized in toto and rather appears to be processed cotranscriptionally by cleavage for poly(A) addition.
Data from 53BP1 Is Limiting for NHEJ Repair in ATM-deficient Model Systems That Are Subjected to Oncogenic Stress or Radiation (2023)

&lt;div&gt;Abstract&lt;p&gt;The DNA damage response (DDR) factors ataxia telangiectasia mutated (ATM) and p53 binding protein 1 (53BP1) function as tumor suppressors in humans and mice, but the significance of their mutual interaction to the suppression of oncogenic translocations &lt;i&gt;in vivo&lt;/i&gt; has not been investigated. To address this question, the phenotypes of compound mutant mice lacking 53BP1 and ATM (Trp53bp1&lt;sup&gt;−/−&lt;/sup&gt;/Atm&lt;i&gt;&lt;sup&gt;−/−&lt;/sup&gt;&lt;/i&gt;), relative to single mutants, were examined. These analyses revealed that loss of 53BP1 markedly decreased the latency of T-lineage lymphomas driven by RAG-dependent oncogenic translocations in Atm&lt;sup&gt;−/−&lt;/sup&gt; mice (average survival, 14 and 23 weeks for Trp53bp1&lt;sup&gt;−/−&lt;/sup&gt;/Atm&lt;sup&gt;−/−&lt;/sup&gt; and Atm&lt;sup&gt;−/−&lt;/sup&gt; mice, respectively). Mechanistically, 53BP1 deficiency aggravated the deleterious effect of ATM deficiency on nonhomologous end-joining (NHEJ)—mediated double-strand break repair. Analysis of V(D)J recombinase-mediated coding joints and signal joints in Trp53bp1&lt;sup&gt;−/−&lt;/sup&gt;/Atm&lt;sup&gt;−/−&lt;/sup&gt; primary thymocytes is, however, consistent with canonical NHEJ-mediated repair. Together, these findings indicate that the greater NHEJ defect in the double mutant mice resulted from decreased efficiency of rejoining rather than switching to an alternative NHEJ-mediated repair mechanism. Complementary analyses of irradiated primary cells indicated that defects in cell-cycle checkpoints subsequently function to amplify the NHEJ defect, resulting in more frequent chromosomal breaks and translocations in double mutant cells throughout the cell cycle. Finally, it was determined that 53BP1 is dispensable for the formation of RAG-mediated hybrid joints in Atm&lt;sup&gt;−/−&lt;/sup&gt; thymocytes but is required to suppress large deletions in a subset of hybrid joints.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Implications:&lt;/b&gt; The current study uncovers novel ATM-independent functions for 53BP1 in the suppression of oncogenic translocations and in radioprotection.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Visual Overview:&lt;/b&gt; &lt;a href="http://mcr.aacrjournals.org/content/11/10/1223/F1.large.jpg" target="_blank"&gt;http://mcr.aacrjournals.org/content/11/10/1223/F1.large.jpg&lt;/a&gt;.&lt;/p&gt;&lt;p&gt;&lt;i&gt;Mol Cancer Res; 11(10); 1223–34. ©2013 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
cDNA cloning and in vitro synthesis of the Dolichos biflorus seed lectin (1987)

The Dolichos biflorus seed lectin contains two structurally related subunits. A cDNA library was constructed using RNA isolated from D. biflorus seeds actively synthesizing the seed lectin. The library was expressed in Escherichia coli using a lambda Charon 16 vector, and lectin-specific antiserum was used to isolate a seed lectin cDNA. Hybridization of the D. biflorus seed lectin cDNA to RNA isolated from seeds actively producing both lectin subunits identifies a single-size RNA of 1100 bases. An oligodeoxyribonucleotide probe, constructed from an amino acid sequence common to both lectin subunits, detects the same size RNA. Translation of seed mRNA in vitro and immunoprecipitation of translation products using a lectin-specific antiserum yields a single polypeptide of slightly higher molecular mass than the largest seed lectin subunit. This seed lectin precursor is indistinguishable from a polypeptide synthesized from mRNA hybrid selected by the seed lectin cDNA. These data support the existence of a single polypeptide precursor for both subunit types of the D. biflorus seed lectin and suggest that differences between the subunit types arise by posttranslational processing.
Process Development for the Synthesis of 5′‐O‐(4,4′‐Dimethoxytrityl)‐N2‐isobutyryl‐2′‐O‐ (2‐methoxyethyl)‐guanosine — A Potential Precursor for the Second Generation Antisense Oligonucleotides: An Improved Process for the Preparation of 2′‐O‐Alkyl‐2,6‐diaminopurine Riboside (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Model Synthesis: A General Procedural Modeling Algorithm (2010)

We present a method for procedurally modeling general complex 3D shapes. Our approach can automatically generate complex models of buildings, man-made structures, or urban data sets in a few minutes based on user-defined inputs. The algorithm attempts to generate complex 3D models that resemble a user-defined input model and satisfy various dimensional, geometric, and algebraic constraints to control the shape. These constraints are used to capture the intent of the user and generate shapes that look more natural. We also describe efficient techniques to handle complex shapes and highlight its performance on many different types of models. We compare model synthesis algorithms with other procedural modeling techniques, discuss the advantages of different approaches, and describe as close connection between model synthesis and context-sensitive grammars.
1002. Silver–hydrocarbon complexes (1964)

W. Featherstone and A. J. S. Sorrie, J. Chem. Soc., 1964, 5235 DOI: 10.1039/JR9640005235
Mapping the Genetic Architecture of Gene Regulation in Whole Blood (2014)

Our data suggest that whole blood is a robust tissue for eQTL analysis and may be used both for biomarker studies and to enhance our understanding of molecular mechanisms underlying gene-disease associations.
Ketoconazole to Reduce the Need for Cyclosporine after Cardiac Transplantation (1995)

After cardiac transplantation, ketoconazole greatly reduced the need for cyclosporine, resulting in substantial cost savings. Ketoconazole also reduced the rates of rejection and infection, without persistent toxic effects. We now use ketoconazole routinely in cardiac-transplant recipients.
Pectus carinatum: When less is more. (2019)

Awareness of pectus carinatum has increased among the medical community over the last several decades, as innovative options for nonsurgical treatments have become more widely known. Management alternatives have shifted from open resective to minimally invasive strategies, and finally, to reshaping the chest using both surgical and non-surgical modalities. We aim to review the evolution of the diagnosis and treatment of pectus carinatum up to its current management.
Energy efficiency optimization in relay-assisted networks with energy harvesting relay constraints (2015)

In this paper we consider data transmission in a decode-and-forward (DF) relay-assisted network in which the relay is energy harvesting (EH) powered while the base station (BS) is power-grid powered. Our purpose is to maximize the BS's energy efficiency (EE) while making full use of the relay's renewable energy and satisfying the specific average throughput requirements. In contrast to existing literature on energy harvesting system which only considers the radio transmission power, we take the static circuit power into account as well. We formulate the EE optimization problem and prove that the EE of the BS and relay are both quasiconvex in the instantaneous transmission rate. Then we divide the complex optimization problem into two point-to-point link level optimization parts and propose an energy-efficient resource allocation (EERA) scheme in which power control and sleep mode management are jointly used. The simulation results demonstrate that EERA may achieve good energy saving effects. We also compare the EE of an energy harvesting relay system with a power-grid powered one and provide more insight into the EE problem of energy harvesting relay system.
Unveiling Interplay Between Pt Single-atoms and Well-defined Anatase TiO2 Under Redox Conditions (2023)

Supported single metal atoms have been extensively investigated in heterogeneous catalysis due to the ultimate utilization efficiency of the noble metal, resulting in cost savings.However, all these single atom catalysts (SACs) studied so far have non-
Glucagon-like peptide 1 and fatty acids amplify pulsatile insulin secretion from perifused rat islets (2002)

Glucose-induced insulin secretion from isolated, perifused rat islets is pulsatile with a period of about 5-10 min, similar to the insulin oscillations that are seen in healthy humans but which are impaired in Type II diabetes. We evaluated the pattern of enhancement by the potent incretin, glucagon-like peptide 1 (GLP-1). GLP-1 increased the amplitude of pulses and the magnitude of insulin secretion from the perifused islets, without affecting the average time interval between pulses. Forskolin and the phosphodiesterase inhibitor isobutylmethylxanthine had the same effect, suggesting that the effect was due to elevated cAMP levels. The possibility that cAMP might enhance the amplitude of pulses by reducing phosphofructo-2-kinase (PFK-2) activity was eliminated when the liver isoform of PFK-2 was shown to be absent from beta-cells. The possibility that cAMP enhanced pulsatile secretion, at least in part, by stimulating lipolysis was supported by the observations that added oleate had a similar effect on secretion, and that the incretin effect of GLP-1 was inhibited by the lipase inhibitor orlistat. These data show that the physiological incretin GLP-1 preserves and enhances normal pulsatile insulin secretion, which may be essential in proposed therapeutic uses of GLP-1 or its analogues.
Control of large 1D networks of double integrator agents: role of heterogeneity and asymmetry on stability margin (2010)

We consider the distributed control of a network of heterogeneous agents with double integrator dynamics to maintain a rigid formation in 1D Euclidean space. The control signal at a vehicle is allowed to use relative position and velocity with its two nearest neighbors. Most of the work on this problem, though extensive, has been limited to homogeneous networks, in which agents have identical masses and control gains, and symmetric control, in which information from front and back neighbors are weighted equally. We examine the effect of heterogeneity and asymmetry on the closed loop stability margin, which is measured by the real part of the least stable pole of the closed-loop system. By using a PDE (partial differential equation) approximation in the limit of large number of vehicles, we show that heterogeneity has little effect while asymmetry has a significant effect on the stability margin. When control is symmetric, the stability margin decays to 0 as $O(1/N^2)$, where $N$ is the number of agents, even when the agents are heterogeneous in their masses and control gains. In contrast, we show that arbitrarily small amount of asymmetry in the velocity feedback gains can improve the decay of the stability margin to $O(1/N)$. Poor design of such asymmetry makes the closed loop unstable for sufficiently large $N$. With equal amount of asymmetry in both position and velocity feedback gains, the closed loop is stable for arbitrary $N$ and the stability margin scaling trend can also be improved to $O(1/N)$, but the sensitivity to disturbance becomes worse. Effect of asymmetry in position feedback gains alone and unequal amount of asymmetry in position and velocity feedback are open problems. Numerical computations are provided to corroborate the analysis.
Mining Time Series Data (2005)

Abstract Some of the problems raised by massive time series databases are briefly considered. They include indexing, clustering, classification, prediction, association detection, summarization, anomaly detection, and segmentation, most of which make explicit or implicit use of a distance measure. A key feature is the approximate representation of time series.
Synergy of Fossil Fuels and Nuclear Energy for the Energy Future (2006)

The paper reviews the methods of producing or upgrading the energy carriers utilizing fossil fuels and nuclear energy, individually or synergistically, for the 21st century when the best-mixed supplies of available primary energies are crucial.
Linear and Non-Linear Magnitude-DFT Estimators under Super-Gaussian Speech Priors (2010)

Although the linear mean-squared error (MSE) complex-DFT estimator, i.e., the Wiener lter , has been known for years, its magnitude-DFT counterpart has never been considered in the context of speech enhancement. Therefore, certain theoretical questions on MSE magnitude-DFT (MDFT) estimators remained unanswered. More specically , it is unknown what the added value is of non-linear over linear MSE MDFT estimators. Furthermore, it has been unknown to which extend the performance of existing MSE MDFT estimators depends on the chosen speech prior, or on the fact that the estimators are non-linear. In this report we present linear MSE MDFT estimators for speech enhancement and perform an analysis by means of objective and subjective experiments of its performance compared to non-linear MSE MDFT estimators. Surprisingly, in contrast to the linear complex-DFT estimator, the presented linear MSE MDFT estimators do depend on the assumed underlying distribution of the speech DFT coefcients. Therefore, for a certain distribution of the speech DFT coefcients, a linear as well as a non-linear MSE MDFT estimator can be obtained. From objective experiments it follows that the performance loss that is obtained by using a linear instead of a nonlinear MSE magnitude estimator is almost negligible. Moreover, from subjective experiments by means of a Mushra listening test is follows that the difference between the non-linear and presented linear MSE MDFT estimator is even statistically not signicant. For practical systems, where estimators are often tabulated and stored in memory, the presented linear estimators might be of interest as the amount necessary memory to tabulate the estimator is highly reduced compared to non-linear MSE MDFT estimators.
Accelerating failure rate of the ASR total hip replacement (2011)

There is widespread concern regarding the incidence of adverse soft-tissue reactions after metal-on-metal (MoM) hip replacement. Recent National Joint Registry data have shown clear differences in the rates of failure of different designs of hip resurfacing. Our aim was to update the failure rates related to metal debris for the Articular Surface Replacement (ASR). A total of 505 of these were implanted. Kaplan-Meier analysis showed a failure rate of 25% at six years for the ASR resurfacing and of 48.8% for the ASR total hip replacement (THR). Of 257 patients with a minimum follow-up of two years, 67 (26.1%) had a serum cobalt concentration which was greater than 7 μg/l. Co-ordinate measuring machine analysis of revised components showed that all patients suffering adverse tissue reactions in the resurfacing group had abnormal wear of the bearing surfaces. Six THR patients had relatively low rates of articular wear, but were found to have considerable damage at the trunion-taper interface. Our results suggest that wear at the modular junction is an important factor in the development of adverse tissue reactions after implantation of a large-diameter MoM THR.
Numerical Modeling of Harmonic Auto-resonance Peniotron (HARP) (1991)

The amplifier source of high efficiency and high power is in demand in the millimeter wave frequency. The gyro-TWT operating at the fundamental cyclotron mode can meet these conditions at the cost of using high magnetic field. The magnetic field can be reduced by operating at high harmonic cyclotron mode but with reduced efficiency. The peniotron was introduced to improve the efficiency, and the HARP does increase it even further. As used in the cyclotron auto-resonance maser (CARM), the same concept of nearly locked resonance condition is able to be used in the HARP when the interaction takes place near the velocity of light line. The frequency remains nearly constant in the dispersion diagram during the evolution passage of decreasing kinetic energy of beam electrons. There is a remarkable difference between the CARM and HARP in their instability characteristics. CARM exhibits a negative mass type of instability and shows a corresponding bunching pattern in the phase. However the HARP follows the drift induced instability due to B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rƒ</sub> x v ⊥. in the geometric space. The nonlinear evolution of the HARP is investigated numerically for the axis encircling electron beam. The set of coupled nonlinear differential equations which govern the self-consistent evolution of the TE modes and the trajectories of an ensemble of electrons in a peniotron are solved subject to usual boundary conditions as in the gyrotron amplifier simulation. The space charge effects are neglected in the analysis. We will present numerical results of the efficiency and power of the HARP for the cylindrical and slotted waveguide structures in this presentation.
ChemInform Abstract: Glycosyl Sulfonylcarbamates: New Glycosyl Donors with Tunable Reactivity. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Roles of stratification in harmful algal bloom ecology (2013)

Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Synthesis Open Science Meeting, 24-26 April 2013, Paris, France
Processing and geometric attribute analysis of 3D PS-converted waves (2005)

Tury Taner defines seismic attributes as “all the information obtained from seismic data, either by direct measurements or by logic or experience-based reasoning.” Seismic attributes typically provide information related to the amplitude, shape, and/or position of the seismic waveform. A common classification of seismic attributes consists of five categories: complex trace attributes, Fourier attributes, time attributes, windowed attributes, and geometric (also called multitrace) attributes. The last category consists of attributes calculated using more than one input seismic trace that provide quantitative information about lateral variations in the seismic data (including coherence, dip/azimuth, and amplitude gradients).
Simulation of LED devices with high CRI (2012)

The possibilities of obtaining CRI LED devices above 90 by simulating the spectra of standard daylight sources and Planck radiators are analyzed.