gebhart/patrepeval huggingface dataset:
Analysis of Protein Co‐Occupancy by Quantitative Sequential Chromatin Immunoprecipitation (2005)

Sequential Chromatin Immunoprecipitation (SeqChIP) is a powerful technique for analyzing the simultaneous association of two different proteins with genomic DNA sequences in vivo. Cellular Protein-DNA complexes are cross-linked with formaldehyde (UNIT ), and are purified via two successive immunoprecipitations, with each immunoprecipitation targeting a different protein. Protein-DNA cross-links are then reversed and DNA sequences of interest are analyzed by quantitative PCR. At each genomic region, calculated SeqChIP co-occupancy values are compared to occupancy values of singly immunoprecipitated samples. The extent of enrichment brought about by the second immunoprecipitation relative to the singly immunoprecipitated sample is directly correlated with the degree of co-occupancy between the two proteins at the genomic location assayed. In principle, the technique is not limited to Saccharomyces cerevisiae. Cells from a wide variety of organisms can be used.
Experimental Neurogenic Bladder in Rats and Effect of Robaveron, a Biological Prepared from Swine Prostate, on It (1986)

An experimental neurogenic bladder was induced in rats by an intraspinal injection of 10% phenol-glycerin solution. The functional and biochemical changes in the bladder were studied in vivo and ex vivo, and the effect of Robaveron, a biological prepared from swine prostate, on these changes were examined. The forced voiding pressure in the control rats which had been caused by the neurogenic bladder was reduced to 1/4-1/5 that of the intact animals. Robaveron recovered a decrease in the pressure, and the effect of 150-250 mg/kg, p.o., corresponded nearly to that of 40 mg/kg, i.m. In the ex vivo study with the strips of isolated neurogenic bladder muscle, the time required to cause 50% of the maximum contraction by transmural electric stimulation prolonged to about four times that of the intact one. Such a prolongation was recovered by Robaveron in a dose-dependent fashion, and the effect of Robaveron given p.o. corresponded to about 1/6 that of the drug given i.m. No change in Ca2+-influx, was found, although Ca2+-efflux was inhibited in the strip of neurogenic bladder muscle. Such an inhibition was significantly relieved in that of animals treated with Robaveron. In the neurogenic bladder rat, the ratio of bladder weight to body weight increased, and the activities of total ChE and AChE in the bladder muscle decreased, on which Robaveron did not show any influence. Phasic and tonic contractions in the strips of intact rat bladder and guinea pig ileum were inhibited by La3+, particularly phasic response in both the strips. Robaveron recovered the inhibition in a dose-dependent fashion. The present study suggests that the urinary dysfunction in neurogenic bladder may be caused not only by nervous disorders but also by changes in the bladder muscle itself. Robaveron was found to be effective for most of such changes. These findings may support the clinical efficacy of Robaveron for improving the attenuated bladder function.
Wake Encounter Flight Tests Performed within S-Wake Project (2003)

We investigated the effect of dexamethasone on parenteral lipid tolerance in 7-day-old extremely low birth weight infants (n = 28) in a randomized, double-blind trial. Serum triglycerides were measured before and after 3 days of dexamethasone or placebo treatment. Infants treated with dexamethasone responded with higher triglyceride concentrations and greater sensitivity to incremental increases in the intravenous lipid dose.
Lysophosphatidylcholines containing polyunsaturated fatty acids were found as sodium-potassium ATPase inhibitors in acutely volume-expanded hog (1987)

Na+, K+-ATPase inhibitors possessing inhibitory activities against the specific binding of ouabain to Na+, K+-ATPase and 86Rb uptake into hog erythrocytes have been purified from the plasma of acutely saline-infused hog. The purifications were performed by a combination of Amberlite XAD-2 adsorption chromatography and four steps of high-performance liquid chromatography with four different types of columns. Fast atom bombardment (FAB) mass and proton NMR spectrometric studies identified the purified substances as gamma-arachidoyl- [LPCA(gamma), 34%], beta-arachidoyl- [LPCA(beta), 4%], gamma-linoleoyl- (LPCL, 33%), and gamma-oleoyl- (LPCO, 25%) lysophosphatidylcholine, expressed in molar ratio in the plasma. Small amounts of gamma-docosapentaenoyl-, gamma-eicosatrienoyl-, and gamma-palmitoyllysophosphatidylcholine were also detected by both FAB mass and 1H NMR spectrometric studies. Only gamma-acyl-LPC's showed inhibitory activities on Na+,K+-ATPase and ouabain-binding activities. These LPC's were effective at 100 microM levels in attaining 50% inhibition of the enzyme activity. The inhibition of Na+,K+-ATPase activity due to these compounds was always more sensitive than that of both ouabain-binding and 86Rb uptake activities. The ouabain-displacing activity in plasma due to these compounds increased with time during saline infusion. The maximal plasma level was approximately 10 times higher than that in the preinfusion plasma sample. Although these results suggest the gamma-acyl-LPC's with long-chain polyunsaturated fatty acids are not simple competitive inhibitors to Na+, K+ -ATPase, these compounds could be implicated in the pathogenesis of the circulation abnormality through the modulation of membrane enzyme.
Role of β3 Integrins in Melanoma Cell Adhesion to Activated Platelets under Flow (1996)

Mechanisms mediating tumor cell attachment to the vessel wall under flow conditions are largely unknown. Therefore we analyzed the ability of human melanoma cells to adhere to an immobilized matrix during blood flow and determined the role of platelets in this process. In a parallel plate flow chamber, M21 melanoma cells were suspended in human blood and perfused over a collagen I matrix at a wall shear rate of 50 s-1 (2 dynes/cm2) to simulate venous flow over a thrombogenic surface. Melanoma cell interaction with the matrix or blood cells and platelets was monitored and quantified by fluorescence and confocal laser microscopy. Despite their ability to adhere to collagen I under static conditions, M21 cells failed to attach directly to this matrix during blood flow. However, they associated with adherent thrombi, and this resulted in stable melanoma cell arrest. Inhibition of platelet activation or platelet integrin alphaIIbbeta3 function abolished M21 cell attachment. Melanoma cell interaction with thrombi was specific and required beta3 integrin expression. M21-L cells which lack integrin alphavbeta3 failed to associate with thrombi and to arrest during blood flow. Transfection of these cells with the integrin subunits alphav or alphaIIb resulted in variants expressing alphavbeta3, as in the wild type, or alphaIIbbeta3. Both variants were able to associate with thrombi and to arrest during blood flow. Therefore, beta3 integrin-mediated binding to activated platelets represents an efficient mechanism for melanoma cell arrest under flow, and this may contribute to the role of platelets in hematogenous metastasis.
Filter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrant (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFilter assay for thymidylate synthetase using 5-fluoro-2'-deoxyuridylate as an active site titrantDaniel V. Santi, Charles S. McHenry, and Evelyne R. PerriardCite this: Biochemistry 1974, 13, 3, 467–470Publication Date (Print):January 1, 1974Publication History Published online1 May 2002Published inissue 1 January 1974https://pubs.acs.org/doi/10.1021/bi00700a011https://doi.org/10.1021/bi00700a011research-articleACS PublicationsRequest reuse permissionsArticle Views48Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Steady-State heat transfer through micro-channels in pressurized He II (2012)

The operation of the Large Hadron Collider superconducting magnets for current and high luminosity future applications relies on the cooling provided by helium-permeable cable insulations. These insulations take advantage of a He II micro-channels network constituting an extremely efficient path for heat extraction. In order to provide a fundamental understanding of the underlying thermal mechanisms, an experimental setup was built to investigate heat transport through single He II channels typical of the superconducting cable insulation network, where deviation from the macro-scale theory can appear. Micro-fabrication techniques were exploited to etch the channels down to a depth of ~ 16 ìm. The heat transport properties were measured in static pressurized He II and analyzed in terms of the laminar and turbulent He II laws, as well as in terms of the critical heat flux between the two regions.
El enfoque de género de las iniquidades en salud desde el prisma de la Bioética (2005)

El trabajo hace un analisis de las iniquidades en salud vistas con un enfoque de genero, desde el prisma de la Bioetica, disciplina que establece un puente entre el hombre y su medio social y natural, a la luz de los valores morales. A partir de la definicion de las iniquidades en salud y como ellas se reflejan en el caso de la mujer, se hace una exposicion del tratamiento a la mujer a lo largo de la historia con enfasis en los aspectos relacionados con la salud sexual y reproductiva, desde la adolescencia y haciendo tambien alusion al abuso, el maltrato y la violencia, para concluir con el debate teorico acerca de los fundamentales principios eticos o de prima fascie, cuya violacion afecta doblemente a la mujer.
Comprehensive Comparisons of Satellite Data, Signals, and Measurements between the BeiDou Navigation Satellite System and the Global Positioning System (2016)

The Chinese BeiDou navigation satellite system (BDS) aims to provide global positioning service by 2020. The combined use of BDS and Global Positioning System (GPS) is proposed to provide navigation service with more stringent requirements. Actual satellite data, signals and measurements were collected for more than one month to analyze the positioning service qualities from both BDS and GPS. In addition to the conversions of coordinate and timing system, five data quality analysis (DQA) methods, three signal quality analysis (SQA) methods, and four measurement quality analysis (MQA) methods are proposed in this paper to improve the integrated positioning performance of BDS and GPS. As shown in the experiment results, issues related to BDS and GPS are resolved by the above proposed quality analysis methods. Thus, the anomalies in satellite data, signals and measurements can be detected by following the suggested resolutions to enhance the positioning performance of the combined use of BDS and GPS in the Asia Pacific region.
Syntheses of cis-Zeatin and Its 9-(2-Deoxy-.BETA.-D-ribofuranosyl) Derivative. A Novel Synthetic Route to the Side Chain at C (6), and Cytokinin Activity. (1992)

cis-Zeatin (3a) and its 9-(2-deoxy-β-D-ribofuranosyl) derivative (3c) have been synthesized from N-[(1, 1-dimethylethoxy)carbonyl]glycine methyl ester (5) in 5 steps by adopting the "α-amino aldehyde/olefination" technology. The new cis-zeatin derivative (3c), its trans isomer (1c), and known trans-zeatin 9-β-D-riboside (1b) were tested for cytokinin activity in the stimulation of chlorophyll biosynthesis in etiolated cucumber cotyledons. The riboside 1b turned out to be the most active cytokinin among them, while the deoxyribosides 1c and 3c showed a marked decrease and a total loss, respectively, of cytokinin activity.
Including the Effects of Weave Draping Within Multiscale Simulations (2017)

This paper will present a general methodology by which weave draping manufacturing simulation results can be utilized to include the effects of weave draping and scissor angle in a structural multiscale simulation. While the methodology developed is general in nature, this paper will specifically demonstrate the methodology applied to a truncated pyramid, utilizing manufacturing simulation weave draping results from ESI PAM-FORM, and multiscale simulation using Altair Multiscale Designer (MDS) and OptiStruct. From a multiscale simulation perspective, the weave draping manufacturing simulation results will be used to develop a series of woven unit cells which cover the range of weave scissor angles existing within the part. For each unit cell, a multiscale material model will be developed, and applied to the corresponding spatial locations within the structural simulation mesh. In addition, the principal material orientation will be mapped from the wave draping manufacturing simulation mesh to the structural simulation mesh using Altair HyperMesh mapping technology. Results of the coupled simulation will be compared and verified against experimental data of the same available via General Motors (GM) Department of Energy (DOE) project.
The persistence of anticardiolipin antibodies is associated with an increased risk of the presence of lupus anticoagulant and anti-β2-glycoprotein I antibodies (2006)

Persistence of aCL positivity is associated with an increased risk of LA/abeta2GPI.
Decreased Basal Ganglia Volume in Cerebral Amyloid Angiopathy (2021)

These findings suggest an independent effect of CAA on basal ganglia tissue loss, indicating a novel mechanism for CAA-related brain injury and neurologic dysfunction.
WOLF–MOOSE INTERACTION ON ISLE ROYALE: THE END OF NATURAL REGULATION? (1999)

Long-term population fluctuations of wolves and moose in Isle Royale National Park, Michigan, are used to evaluate a central tenet of the “natural regulation” concept commonly applied by the National Park Service (NPS) in the United States, namely, that wild cervid populations exhibit density dependence which, even in the absence of large predators, will stabilize population growth. This tenet, restated as a hypothesis, is rejected based on moose population response to a chronic wolf decline. In 1980–1996 with wolf numbers down, partly due to introduced disease, moose numbers increased to a historic high level. There was insufficient density dependence in moose reproduction and mortality to stabilize moose numbers. In 1996 moose suffered a crash; 80% died, primarily from starvation. These fluctuations, along with the possibility that the highly inbred wolf population may become extinct, will challenge NPS policy. The long-standing NPS management tradition of nonintervention may not be compatible with the current policy that stresses maintenance of natural ecological processes, such as a predator–prey system.
Device-Free Human Sensing and Localization in Collaborative Human–Robot Workspaces: A Case Study (2015)

Modern robot manufacturing is fostering the implementation of hybrid production systems characterized by human-robot cooperative tasks. Safety technologies for workers protection require advanced sensing capabilities and flexible solutions that are able to monitor the movements of the operator in proximity of moving robots. This paper proposes the use of wireless device-free localization (DFL) methods and architectures to detect and track a human worker in a cooperative human-robot industrial workspace. The DFL system is composed of groups of massively interacting small, low-cost, embedded radio-frequency (RF) transceivers that perform received power measurements. These devices are anchored in fixed peripheral locations of the plant and provide the localization of the worker, who peculiarly carries neither wireless active devices (device-free) nor specific tracking sensors (sensor-free sensing). Operator motion is, in fact, estimated by tracking the perturbations of the radio field induced by the human body, considering the effect of concurrently moving robot as non-stationary interference. The proposed localization and detection algorithm is based on the jump linear Markovian system and the interactive multiple model method, and its positioning accuracy has been validated by experiments performed inside a robotic cell of an industrial test plant. The proposed DFL system has been implemented by employing IEEE 802.15.4 RF devices operating at 2.4 GHz and integrated into a software safety architecture. Finally, a software toolset has been designed to predict DFL accuracy, to verify experimental measurements, and also to support the integration with preinstalled industrial sensors to increase the accuracy of the augmented system.
A Simple Procedure for Determination of Aminocyclopyrachlor and Aminopyralid in Soil, Corn Meal, and Soy Meal using Liquid Chromatography/Tandem Mass Spectrometry (2018)

An analytical method has been developed that efficiently extracts aminocyclopyrachlor and aminopyralid from a variety of matrices including soil, soy meal, and corn meal without the need of time-consuming matrix cleanup steps such as solid phase extraction. Following extraction, the samples are analyzed by positive mode LC-ESI-MS/MS. Spiking studies were conducted at low- and intermediate-concentrations to determine the method detection limit as well as to evaluate the accuracy and precision of the method. Generally, aminocyclopyrachlor and aminopyralid recoveries were acceptable following extraction from soil, soy meal, and corn meal.https://doi.org/10.21423/jrs-v06n02p001 (DOI assigned 1/24/2019)
Molecular species of phosphatidylethanolamine from continuous cultures of Saccharomyces pastorianus syn. carlsbergensis strains (2006)

Saccharomyces pastorianus syn. carlsbergensis strain 34/70 is well known to be the most used strain for lager beer production. The difference between this strain and very closely related strain 34/78 is the latter's greater flocculating character. This single physiological trait can cause technical difficulties in beer production. The aim of this study was to determine whether lipid analysis by a combination of thin layer chromatography (TLC) with electrospray ionization mass spectrometry (ESI-MS) could be used as a strain-typing technique in order to distinguish S. pastorianus syn. carlsbergensis strain 34/70 from strain 34/78. Both strains (34/70 and 34/78) were harvested after continuous culture under standard conditions. Polar lipids were then extracted from lyophilized cultures and analysed by TLC in order to separate phospholipid families. Phosphatidylethanolamine (PE) was extracted and investigated using ESI-MS, to gain further information on individual molecular species. Using TLC analysis, lipids were separated corresponding to standards for PE, phosphatidylcholine (PC), phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA) and sphingomyelin (SM). ESI-MS of the PE band, separated by TLC, showed that electrospray mass spectra were highly reproducible for repeat cultures. Novel findings were that both brewing strains displayed major phospholipid peaks with m/z 714, PE (34 : 2) m/z 742, PE (36 : 2) and m/z 758, PE (37 : 1). However, strain 34/78 had additional peaks of m/z 700, PE (33 : 2) and m/z 728, PE (35 : 2). Strain 34/70 had an extra peak with m/z 686 PE (32 : 2). We conclude that combined TLC/ESI-MS can distinguish between S. pastorianus syn. carlsbergensis 34/70 and 34/78 and may be a useful typing technique for differentiation of closely related yeast strains. This novel approach may aid quality assurance and could be suitable for yeast collections and larger industrial companies.
Accuracy of Urine Cytology and the Significance of an Atypical Category (2009)

The "atypical urothelial cell" cytologic category is nonstandardized. We subclassify atypical cases to "atypical, favor a reactive process" or "atypical, unclear if reactive or neoplastic." We evaluated the predictive significance of atypical cases by looking at their histologic follow-up. Among the 1,114 patients and 3,261 specimens included, 282 specimens had histologic follow-up. An atypical diagnosis did not carry a significant increased risk of urothelial neoplasia compared with the benign category. Although an "atypical unclear" diagnosis carried a higher rate of detection of high-grade cancer on follow-up biopsy in comparison with "atypical reactive" or "negative" diagnoses (26/58 [45%] vs 15/52 [29%] and 16/103 [15.5%], respectively), this difference was not statistically significant. These results suggest that dividing atypical cases into 2 categories based on the level of cytologic suspicion of cancer does not add clinically relevant information within the atypical category. They also raise the question of the significance of the atypical category altogether.
Regularized Transport Between Singular Covariance Matrices (2020)

We consider the problem of steering a linear stochastic system between two endpoint <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">degenerate</i> Gaussian distributions in finite time. This accounts for those situations in which some but not all of the state entries are uncertain at the initial, <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$t=0$</tex-math></inline-formula> , and final time, <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$t=T$</tex-math></inline-formula> . This problem entails nontrivial technical challenges, as the singularity of terminal state covariance causes the control to grow unbounded at the final time <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$T$</tex-math></inline-formula> . Consequently, the entropic interpolation ( <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Schrödinger bridge</i> ) is provided by a diffusion process, which is not <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">finite energy</i> , thereby placing this case outside of most of the current theory. In this article, we show that a feasible interpolation can be derived as a limiting case of earlier results for nondegenerate cases, and that it can be expressed in closed form. Moreover, we show that such interpolation belongs to the same <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">reciprocal class</i> of the uncontrolled evolution. By doing so, we also highlight a time symmetry of the problem, contrasting dual formulations in the forward and reverse time directions, where in each, the control grows unbounded as time approaches the endpoint (in the forward and reverse time direction, respectively).
ChemInform Abstract: VERBINDUNGEN MIT PSYCHOTROPER WIRKSAMKEIT, 7,12‐DIHYDRO‐5H‐DIBENZO(C,F)THIOCINE (1971)

Abstract Die Bromierung des Benzophenons (I) mit N‐Brom‐succinimid führt zum Dibromid (II), das zum Dibenzothiocinon (III) (18,8% Ausbeute) cyclisiert wird.