gebhart/patrepeval huggingface dataset:
Conformational flexibility of bacterial RNA polymerase (2002)

The structure of Escherichia coli core RNA polymerase (RNAP) was determined by cryo-electron microscopy and image processing of helical crystals to a nominal resolution of 15 A. Because of the high sequence conservation between the core RNAP subunits, we were able to interpret the E. coli structure in relation to the high-resolution x-ray structure of Thermus aquaticus core RNAP. A very large conformational change of the T. aquaticus RNAP x-ray structure, corresponding to opening of the main DNA/RNA channel by nearly 25 A, was required to fit the E. coli map. This finding reveals, at least partially, the range of conformational flexibility of the RNAP, which is likely to have functional implications for the initiation of transcription, where the DNA template must be loaded into the channel.
Clinical Outcomes following Left Atrial Appendage Occlusion: A Single-Center Experience (2020)

This observational study showed no statistically significant difference in bleeding risk related to initial antithrombotic choice. Smaller device sizes were associated with thromboembolic events, and longitudinal PDL assessment using transesophageal echocardiography showed these frequently do not decrease in size. Larger studies are needed.
Accuracy comparison between two microcontroller-embedded R-wave detection methods for heart-rate variability analysis (2015)

Analysis of heart rate variability, which is calculated using the R-R intervals (RRI) of electrocardiogram (ECG), provides beneficial information for both clinical and healthcare diagnoses. To achieve the required accuracy for RRI measurement using the wearable telemetery system, two R-wave detection methods (one based on voltage threshold and another that adopts differential peak detection) were developed for implementation in a low-power microcontroller integrated into a wearable telemeter. Accuracy of these methods were compared using a clinical-grade ECG measurement system to evaluate the systematic errors of the proposed methods by correlation and Bland-Altman analyses.
Influence of NaCl and pH on intracellular enzymes that influence Cheddar cheese ripening (1997)

The influence of NaCl and reduced pH was determined for aminopeptidase, lipase/esterase and methanethiol-producing capability in selected lactic acid bacteria and brevibacteria in simulated cheese-like conditions. The observations on simulated cheese-like conditions were confirmed in 60% reduced-fat Cheddar cheese. The activity of each enzyme decreased with NaCI addition and when the pH was reduced to approximate Cheddar chee se conditions (5% NaCI, pH 5.2). Residual intracellular aminopeptidase activity was dominated by general aminopeptidase activity (aminopeptidase N and/or aminopeptidase C) in laboratory, simulated cheese-like conditions, and 60% reduced-fat Cheddar cheese curd. During chee se aging, totallipase/esterase activity peaked at 120 d then decreased, ev en though starter culture populations remained high. Methanethiol-producing capability occurred under cheese-like conditions in whole cells, but not in cell-free extracts. Met and Met-containing peptides induced methanethiol-producing capability for 2-3 generations and could be re-induced later in the growth cycle of Brevibacterium linens BL2. Aminopeptidase and lipase/esterase activity in reduced fat cheese were not correlated to an increase in Cheddar-type flavar, but a culture's methanethiol-producing capability was associated with higher chee se consumer preference scores. Results suggest that use of cheese-like conditions may aid in selecting cultures to
A techniques for recognition of a human faces on eye with python (2021)

Human face recognition is a field of study in Computer Vision, and is also considered a research area of Biometrics (similar to fingerprint recognition, or iris recognition). In terms of general principles, facial recognition has a great resemblance to fingerprint recognition and iris recognition, but the difference lies in the specific extraction step of each field. While fingerprint and iris recognition has reached maturity, which is widely applicable in practice, facial recognition remains challenging and remains an interesting area of research with many. people. Compared to fingerprint and iris recognition, facial recognition has a richer data source (you can see human faces in any photo or video clip related to people online) and requires less more controlled interaction (to perform fingerprint or iris recognition, human input requires cooperation in a controlled environment). Currently, the face recognition methods are divided into many directions according to different criteria: still image based FR (2D) recognition is the most popular, but the future will probably be. 3D (because the layout of many 2D cameras will give 3D data and give better and more reliable results), it can also be divided into two directions: doing with image data and doing with video data.
Biosensors — from concept to commercialisation (1987)

Rosemary Albinson, a staff scientist with Cambridge Consultants Ltd, discusses how biosensors are best developed and commercially exploited.
Regenerating Codes: A System Perspective (2012)

The explosion of the amount of data stored in cloud systems calls for more efficient paradigms for redundancy. While replication is widely used to ensure data availability, erasure correcting codes provide a much better trade-off between storage and availability. Regenerating codes are good candidates for they also offer low repair costs in term of network bandwidth. While they have been proven optimal, they are difficult to understand and parameterize. In this paper we provide an analysis of regenerating codes for practitioners to grasp the various trade-offs. More specifically we make two contributions: (i) we study the impact of the parameters by conducting an analysis at the level of the system, rather than at the level of a single device, (ii) we compare the computational costs of various implementations of codes and highlight the most efficient ones. Our goal is to provide system designers with concrete information to help them choose the best parameters and design for regenerating codes.
Bi-directional coupling in nonlinear waveguides for absolute timing determination (2001)

Summary form only given. Future broadband optical networks will work at bit rates exceeding 100 GHz through time-division multiplexing and wavelength-division multiplexing. For signal recovery a high-speed clock recovery or, sub-clock recovery scheme is required. For 3R signal regeneration the recovered clock is needed for retiming of the optical signal. It is conventional to use a nonlinearity with a fast recovery time to determine temporal coincidence such as four wave mixing in a semiconductor optical, amplifier, or saturable absorption in an injection locked laser. Schemes based on devices with slow nonlinear recovery times, such as an electroabsorption modulator, have been realized by applying an external electrical modulation. The optical arrangement presented monitors the difference between the nonlinear transmission of two pulses counter-propagating through a slow nonlinear medium, thus rapidly providing sufficient information to immediately determine the absolute timing error.
Anti-endothelial cell antibodies (AECA) in patients with systemic vasculitis: our research using proteomics (2010)

To identify target antigens of AECA and to establish a standardized method for measuring AECA would be helpful in the search for a possible pathophysiological role of AECA in systemic vasculitis.
Prevention of experimental autoimmune encephalomyelitis in DA rats by grafting primary skin fibroblasts engineered to express transforming growth factor-β1 (2004)

To determine whether primary fibroblasts producing latent transforming growth factor beta1 (TGF-beta1) are capable of down-regulating experimental autoimmune encephalomyelitis (EAE), a retroviral vector TGF-beta1-pBabe-neo (-5'UTR) was used for efficient gene transfer into primary skin fibroblasts of DA rats. After heat activation, conditioned medium from the transduced fibroblasts was found to inhibit significantly in vitro proliferation of lymphocytes from lymph nodes of DA rats with EAE. Intraperitoneal administration of TGF-beta1-transduced fibroblasts into DA rats during the priming phase of EAE resulted in a significant reduction in mortality and in the mean clinical and EAE scores versus the control immunized animals treated with non-transduced fibroblasts.
Communicative Language Teaching in Malaysian ESL Context: A Qualitative Exploration into In-service Teachers' Beliefs and Practices (2021)

In contrast to the plethora of research on teachers' beliefs and practices to implement communicative language teaching (CLT), research into in-service primary school teachers' beliefs has earned less focus to evaluate its impacts on their instructional and evaluation practices, particularly in Malaysian ESL context. This qualitative study intended to mitigate the knowledge gap by investigating prevalent communicative language teaching practices in the primary schools in Malaysia. Analysis of our survey and interview data reveals some common problems that affect the proper implementation of CLT. Although teachers faced challenges and had their shortcomings, they endeavored to integrate various instructional and assessment practices to improve students' competency on all four language skills. The study also highlights some key issues which may provide practical implications for the application of CLT in Malaysian primary schools.
Transplantation of Kidneys From Donors Whose Hearts Have Stopped Beating (1998)

No AccessJournal of UrologyUrological Survey: Abstracts: Kidney Transplantation and Renovascular Hypertension1 Sep 1998Transplantation of Kidneys From Donors Whose Hearts Have Stopped Beating Y.W. Cho, P.I. Terasaki, J.M. Cecka, and D.W. Gjertson Y.W. ChoY.W. Cho , P.I. TerasakiP.I. Terasaki , J.M. CeckaJ.M. Cecka , and D.W. GjertsonD.W. Gjertson View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62854-4AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Transplantation of Kidneys From Donors Whose Hearts Have Stopped Beating." The Journal of Urology, 160(3 Part 1), pp. 954–955 Department of Surgery, UCLA School of Medicine, Los Angeles, California© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 160Issue 3 Part 1September 1998Page: 954-955 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information Y.W. Cho More articles by this author P.I. Terasaki More articles by this author J.M. Cecka More articles by this author D.W. Gjertson More articles by this author Expand All Advertisement PDF downloadLoading ...
Optical spectroscopy of GaN microcavities with thicknesses controlled using a plasma etchback (2001)

The effect of an etch-back step to control the cavity length within GaN-based microcavities formed between two dielectric Bragg mirrors was investigated using photoluminescence and reflectivity. The structures are fabricated using a combination of a laser lift-off technique to separate epitaxial III-N layers from their sapphire substrates and electron-beam evaporation to deposit silica/zirconia multilayer mirrors. The photoluminescence measurements reveal cavity modes from both etched and nonetched microcavities. Similar cavity finesses are measured for 2.0 and 0.8 μm GaN cavities fabricated from the same wafer, indicating that the etchback has had little effect on the microcavity quality. For InGaN quantum well samples the etchback is shown to allow controllable reduction of the cavity length. Two etch steps of 100 nm are demonstrated with an accuracy of approximately 5%. The etchback, achieved using inductively coupled plasma and wet chemical etching, allows removal of the low-quality GaN nucleation layer, control of the cavity length, and modification of the surface resulting from lift-off.
Preparation and catalytic oxidizing potential of polymer supported chelating amine and Schiff base complexes (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and catalytic oxidizing potential of polymer supported chelating amine and Schiff base complexesRussell S. Drago, John Gaul, Alan Zombeck, and Darel K. StraubCite this: J. Am. Chem. Soc. 1980, 102, 3, 1033–1038Publication Date (Print):January 1, 1980Publication History Published online1 May 2002Published inissue 1 January 1980https://pubs.acs.org/doi/10.1021/ja00523a021https://doi.org/10.1021/ja00523a021research-articleACS PublicationsRequest reuse permissionsArticle Views740Altmetric-Citations110LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development Of Technologies For Production Of High Quality Grazing Incidence X-Ray Mirrors On Graphite Epoxy Substrates (1989)

The design concept of the mirrors for the ESA high throughput x-ray spectroscopy mission XMM is characterized by thin (&lt; 1 mm) graphite epoxy shells as mirror structure and a replication technique to produce the mirror surface. The mirror microroughness is specified to &sigma; &lt; 0.5 nm (r.m.$), profile errors shall not exceed + 2.5 arc sec. An ESA research study has been performed by Zeiss and subcontractors (Dornier GmbH, Max Planck Institut fUr Extraterrestrische Physik/ Garching (MPE), University of Leicester, X-ray Astronomical Group, Dept. of Physics) to clarify whether graphite epoxy mirror structures are able to meet the tight XMM mirror figuring and surface requirements and which measures have to be taken to overcome the typical problems associated with composite materials used as mirror substrates:&bull; Print-through of fibres and inner structure &bull;Short term/long term stability&bull; Water absorption and outgassing. The starting conditions and progress during development are described. The present status of optical and x-ray performance of replicated flat graphite epoxy mirrors is reported and aspects for further improvement of graphite epoxy mirrors will be discussed.
Petrov-Galerkin finite elements in time for rigid-body dynamics (1994)

The problem of rigid-body rotational dynamics is examined in the context of a novel Petrov-Galerkin mixed finite-element-in-time formulation. The present approach crucially differs from other formulations in the choice of the test functions that weight the constitutive equations in an integral average sense. Within this framework, two second-order accurate time-stepping algorithms are derived: the first attains a slightly greater accuracy than the second when compared with the analytical solution of a free-tumbling rigid body with an axis of material symmetry, but in general it does not preserve energy for force-free motion; the second is marginally less accurate, but it is associated with a discrete conservation law of the kinetic energy. Numerical simulations are presented that confirm the results of the analysis and illustrate the excellent performance of the proposed numerical approach.
Supplementary Table 1 from High-Risk Endometrial Carcinoma Profiling Identifies TGF-β1 as a Key Factor in the Initiation of Tumor Invasion (2023)

Supplementary Table 1 from High-Risk Endometrial Carcinoma Profiling Identifies TGF-β1 as a Key Factor in the Initiation of Tumor Invasion
Abstract A94: Identification of potent, insurmountable A2a antagonists for modulation of the tumor microenvironment (2020)

Abstract Adenosine is elevated in the tumor microenvironment and plays a critical role in suppressing T-cell function through high-affinity interaction with the A2a receptor. Genetic deficiency of A2a in mice is associated with enhanced cytotoxic responses and reduced tumor burden in syngeneic models. These effects are mimicked by small-molecule A2a antagonists and some of these compounds are currently being evaluated in clinical trials for the treatment of solid tumors, particularly in combination with checkpoint inhibitors. However, high levels of adenosine in the tumor microenvironment may pose a challenge for the development of A2a antagonists unless they possess the appropriate pharmaceutical profile. A series of potent antagonists were identified that displayed sub-nanomolar binding activity and selectivity over other adenosine receptors. Within this series, compounds were identified that maintained activity across a broad range of adenosine concentrations. These compounds behaved as insurmountable antagonists in a functional assay utilizing CHO cells expressing human recombinant A2a and displayed slow dissociation kinetics in a FRET-based receptor-binding assay. This series is exemplified by ARX1598, which had a Ki of 0.08nM in the A2a receptor-binding assay and an estimated KB of 0.06 nM in the recombinant cell-based assay. Schild analysis revealed that this compound behaved as an insurmountable antagonist and had a markedly slower off-rate than comparator competitive antagonists. The adenosine receptor agonist NECA suppressed cytokine production by peripheral blood mononuclear cells activated by anti-CD3/anti-CD28. ARX1598 prevented NECA-mediated suppression of cytokine production by human peripheral blood mononuclear cells (EC50= 6 nM) and prevented elevation of pCREB induced by high concentrations of NECA (3μM) in human whole blood (IC50=22nM). The potency of ARX1598 in the human whole blood assay was 50-100 times higher than comparator compounds currently being evaluated in cancer trials. These data illustrate that it is possible to identify potent compounds that have the potential to modulate the adenosine pathway in the tumor microenvironment. Citation Format: Roy Pettipher, Jonathan White, Viral Patel, Ben Moulton, Soraya Pores, Marta Assuncao, Karolina Gherbi, K. Sengmany, Elisabeth Rosethorne, Peter Finan, Steven Charlton, Clive McCarthy. Identification of potent, insurmountable A2a antagonists for modulation of the tumor microenvironment [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2018 Nov 27-30; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(4 Suppl):Abstract nr A94.
Data-driven Workflow Management by utilising BPMN and CPN in IIoT Systems with the Arrowhead Framework (2019)

Workflow management is realised in manufacturing at the Enterprise- and Production (workstation) levels. The characteristics of business processes in these levels differ enough to prevent the adoption of a conventional modelling or implementation solution. The conceptualisation of an adequate and adaptable workflow management model that over-arches the heterogeneous business process levels is challenging. However, one of the encouragements of Industry 4.0 is to provide easy-to-use models and solutions that enable the effective implementation of production goals.The current paper addresses this challenge and - as a proof-of-concept - it demonstrates how the goals mentioned above can be achieved by combining the different manufacturing process models. To accomplish this, a workflow control engine needs to be designed and standardised respectively. Arrowhead is an IIoT (Industrial IoT) framework that dynamically and flexibly supports automated manufacturing processes following Industry 4.0 expectations. This paper describes how its workflow management system, i.e., the Workflow Choreographer implements automated production. Furthermore, it details what the functions of this system are and how it applies and combines BPMN and CPN in practice to provide a solution for the given challenge.To verify its feasibility, the paper showcases a demo application of the concept as well.
Using historical objective response rates to design single-arm phase II trials in patients with recurrent glioblastoma (2023)

In the past 30 years, only temozolomide has been found to significantly prolong survival in patients with glioblastoma.This drug adds about 6 months to overall survival and does not result in cure.It is effective primarily in the 40% of glioblastomas in adults with MGMT-methylated tumors and in the 12% with IDH mutations.Clinical trials of dose intensification, immune therapies, biologic therapies, and antiangiogenic agents have not impacted survival. 1Nextgeneration sequencing has identified abundant mutations but clinical studies targeting these have been disappointing with responses seen primarily in rare patients with BRAF mutations and NTRK fusions. 2 This lack of progress has been documented by a plethora of negative randomized phase III trials prompting formal discussion as to how better screen therapies before conducting large phase II and III studies. 3n addition, recommendations emerged suggesting that randomized phase II studies be prioritized over single-arm phase II studies to reduce the frequency of negative phase III trials.However, the inclusion of an internal control arm is often less appealing to patients and clinicians and requires a major increase in sample size, time to completion, and cost in a disease which has been very resistant to therapy. 4 The study by Ellingson et al. reported in this issue of Neuro-Oncology seeks to standardize the design of single-arm phase II trials in patients with recurrent glioblastoma. 5In this study, historical data on objective response rates is used to define "active" drugs and to suggest ideal trial sizes.These investigators retrospectively reviewed recorded radiographic response and survival data from the clinical trial records of nearly 4800 patients with recurrent glioblastomas.Patients included in this analysis received cytotoxic chemotherapy, biologic, anti-angiogenic, and immunotherapy agents.Response rates were calculated and analyzed with survival data.The authors conclude that "single-arm phase II studies in recurrent glioblastoma should have a target overall response rate of >25% and
Supplementary Figure 1 from Curing Mice with Large Tumors by Locally Delivering Combinations of Immunomodulatory Antibodies (2023)

&lt;p&gt;Lymphocyte components of mice transplanted with tumor cells.&lt;/p&gt;
Self-Biased 2.4 GHz CMOS RF-to-DC Converter with 80% Efficiency and −22.04 dBm Sensitivity for Wi-Fi Energy Harvesting (2019)

One of the significant disadvantages of RF energy harvesting is having a low power density in comparison to other ambient energy sources. The rectifier is the core of an RF energy harvesting system since it converts and boosts weak RF power to usable DC power. This study introduces a design of an efficiency enhanced RF-to-DC power converter with impedance matched to 50 Ω. The circuit design is based on two circuit design architectures, namely: the fully cross-coupled rectifier and self-biased technique, and the combined with LC matching circuit to obtain high power conversion efficiency. The design simulation is implemented using 65 nm CMOS Technology process. The performance of the proposed circuit design has achieved a peak power conversion efficiency of 80% at -14.4 dBm with a minimum input power of -22.04 dBm at 2.4 GHz for a load resistance of 20 kΩ and 10 pF load.