gebhart/patrepeval huggingface dataset:
CooB is required for assembly but not transport of CS1 pilin (1992)

CS1 pili are filamentous proteinaceous appendages found on many enterotoxigenic Escherichia coli (ETEC) strains isolated from human diarrhoeal disease. They are thought to effect colonization of the upper intestine by facilitating binding to human ileal epithelial cells. We have identified a gene, cooB, which lies directly upstream of cooA, the gene that encodes the major structural CS1 protein. When translated in vitro, the protein product of cooB migrates in sodium dodecyl sulphate/polyacrylamide gel with an apparent molecular mass of 26 kDa, which is consistent with that predicted from its DNA sequence. We constructed a mutant allele (cooB-1) by insertion of the omega fragment, which inhibits transcription and translation, into the cooB gene in vitro. In a derivative of an ETEC strain with the cooB-1 mutation (JEF100) and a plasmid that encodes Rns (pEU2030), the positive regulator required for CS1 expression, no cooB and a greatly reduced level of cooA product was detectable in total cell extracts. The reduction of cooA in this strain appears to result from polarity of the cooB mutation because introduction of the wild-type cooA gene in trans causes production of CooA protein, which is found in cell pellet extracts, in extracts containing only surface proteins and in the culture supernatant. Therefore, in the absence of CooB, CooA is stable and it is transported through both inner and outer membranes. However, the cooB-1 strain with cooA in trans does not cause haemagglutination of bovine erythrocytes (the model system used to assay adherence mediated by coli surface antigen 1 (CS1) pili).(ABSTRACT TRUNCATED AT 250 WORDS)
Small, fast, and tough: Shrinking down integrated elastomer transducers (2016)

We review recent progress in miniaturized dielectric elastomer actuators, sensors and energy harvesters. We focus primarily on configurations where the large strain, high compliance, stretchability and high level of integration offered by dielectric elastomer transducers provide significant advantages over other mm or m-scale transduction technologies. We first present the most active application areas, including: tunable optics, soft robotics, haptics, micro fluidics, biomedical devices and stretchable sensors. We then discuss the fabrication challenges related to miniaturization, such as thin membrane fabrication, precise patterning of compliant electrodes, and reliable batch fabrication of multilayer devices. We finally address the impact of miniaturization on strain, force and driving voltage, as well as the important effect of boundary conditions on the performance of mm-scale DEAs.
Log-based intrusion detection for MANET (2012)

Ad hoc networks operate mostly over open environments and are hence vulnerable to a large number of threats. This calls for providing advanced intrusion detection. To meet this requirement, we introduce IDAR, a signature- and log-based distributed intrusion detector dedicated to ad hoc routing protocols. Contrary to existing systems that observe packets, IDAR analyses the logs generated by the OLSR protocol and identifies patterns of misuse. This detector copes with the resource-constraints of devices by providing distributed detection. In particular, depending on the level of suspicion/gravity involved, in-depth cooperative investigation is launched. Simulation shows limited bandwidth usage, high detection and low false positives.
Intraoperative Spatial Frequency Domain Diffuse Optical Tomography with Indocyanine Green (ICG) Fluorescence Contrast (2016)

We report instrumentation and methods for depth sensitive imaging of tumor fluorescence in an intraoperative setting with spatial frequency domain imaging. We show preliminary reconstruction results, with simulation, using an analytic inversion method.
Photocurrent generation by immobilized cyanobacteria via direct electron transport in photo-bioelectrochemical cells (2014)

Cyanobacteria possess unique and exciting features among photosynthetic microorganisms for energy conversion applications. This study focuses on production of direct electricity using a cyanobacterium called Nostoc sp. (NOS) as a photo-biocatalyst immobilized on carbon nanotubes on the anode of photo-bioelectrochemical cells. By illuminating with light (intensity 76 mW cm(-2)) the NOS immobilized on a carbon nanotube (CNT) modified electrode generated a photocurrent density of 30 mA m(-2) at 0.2 V (vs. Ag/AgCl). The contribution of different photosynthetic pigments in NOS to the light capture was analyzed and chlorophyll-a was found to be the major contributor to light capture followed by phycocyanin. Further investigation using a set of inhibitors revealed that the electrons were redirected predominantly from PSII to the CNT through the plastoquinone pool and quinol oxidase. A rudimentary design photosynthetic electrochemical cell has been constructed using NOS/CNT on the anode and laccase/CNT on the cathode as catalysts. The cell generated a maximum current density of 250 mA m(-2) and a peak power density of 35 mW m(-2) without any mediator. By the addition of 1,4-benzoquinone as a redox mediator, the electricity generation capability was significantly enhanced with a current density of 2300 mA m(-2) and a power density of 100 mW m(-2). The power densities achieved in this work are the highest among 'non-engineered' cyanobacteria based electrochemical systems reported to date.
Phyllogenetically conserved epitopes of the keratin 8 polypeptide recognized by a novel set of monoclonal antibodies. (1991)

In an attempt to raise a set of monoclonal antibody (MAb) probes for the study of biology and diagnostic value of human keratin No. 8, a series of hybridomas was prepared and their characteristics investigated by immunohistochemistry and immunoblotting. The polypeptide specificities of individual MAbs ranged from monospecificity for keratin No. 8 (MAbs C-15, C-23, C-36, C-43, C-47, C-51, and C-61) up to the "pan-keratin" MAbs C-11 and C-66 recognizing a broad range of keratins. The target epitopes of the remaining 4 MAbs (C-10, C-22, C-50, C-69) appear to be shared by various pairs of keratin polypeptides. The immunohistochemical investigation of tissues and/or cultured cells from 10 animal species revealed considerable variability of the interspecies crossreactivity of individual MAbs. The target epitopes of 5 MAbs appear to be phyllogenetically conserved from man to Xenopus, 6 of 12 MAbs tested show reactivity with several mammalian species (but not chicken or Xenopus) and the MAb C-15 reacts with human and sheep keratin only. The data obtained demonstrate that at least 9, and possibly as many as 12, nonidentical epitopes of the human keratin 8 polypeptide can be distinguished by this set of antibodies. The results of the present study suggest that our MAbs represent a significant contribution to the list of reagents applicable in both research of intermediate filament (IF) biology and diagnostic histopathology.
109 EFFECTS OF VITRIFICATION PROCEDURES ON SUBSEQUENT DEVELOPMENT AND ULTRASTRUCTURE OF IN VITRO -MATURED SWAMP BUFFALO ( BUBALUS BUBALIS ) OOCYTES (2006)

The purpose of this study was to investigate the effects of 2 vitrification procedures on the developmental capacity and ultrastructural changes of matured swamp buffalo ( Bubalus bubalis ) oocytes. In vitro -matured (IVM) oocytes were vitrified by using 35% and 40% ethylene glycol (EG) as vitrification solution (VS) for solid surface vitrification (SSV) and in-straw vitrification (ISV), respectively. Survival rate of vitrified–warmed oocytes was evaluated on the basis of homogeneous cytoplasm, membrane integrity, and complete zona pellucida. All developmental data were analyzed using chi-square analysis. P &amp;lt; 0.05 was considered significant. The blastocyst rates of parthenogenetic vitrified–warmed oocytes were significantly higher with SSV (89.3% and 13.6%, respectively) than with ISV (81.8% and 5.5%, respectively). However, they were still significantly lower than those of control (100% and 34.2%, respectively). For examining the ultrastructural changes, fresh VS-exposed (ISV and SSV), and vitrified–warmed oocytes were processed for transmission electron microscopy. In VS-exposed oocytes, reduction of microvilli abundance and damage of mitochondrial membrane were found only in the ISV group. In vitrified–warmed oocytes, however, it was clear that both methods of vitrification induced profound ultrastructural modifications to microvilli, mitochondria, oolemma, and cortical granules as well as to the size and position of vesicles. Damaged mitochondria were, however, more abundant in ISV vitrified oocytes than in SSV vitrified oocytes, which correlated with the developmental data, showing the superiority of the SSV method. This study demonstrated for the first time the feasibility of vitrification of IVM swamp buffalo oocytes.
Myozap, a Novel Intercalated Disc Protein, Activates Serum Response Factor–Dependent Signaling and Is Required to Maintain Cardiac Function In Vivo (2010)

Taken together, these findings reveal Myozap as a previously unrecognized component of a Rho-dependent signaling pathway that links the intercalated disc to cardiac gene regulation. Moreover, its subcellular localization and the observation of a severe cardiac phenotype in zebrafish, implicate Myozap in the pathogenesis of cardiomyopathy.
Antitumor Activity of Bacillus natto. III. Isolation and Characterization of a Cytolytic Substance on Ehrlich Ascites Carcinoma Cells in the Culture Medium of Bacillus natto KMD 1126 (1972)

There were at least two kind of cytolytic substances on Ehrlich ascites carcinoma cells in the culture medium of Bacillus natto KMD 1126. One was adsorbed on carbon, eluted with EtOH, and large molecule by Sephadex G 25 gel filtration, but the other was not adsorbed on carbon and small molecule by Sephadex G 25 gel filtration. The former was stable on wide range of pH and temperature. This cytolytic substance was isolated and its chemical structure was examined by elementary analysis, infrared spectrum, nuclear magnetic resonance, and mass spectroscopy and study of decomposed products. As the results, the cytolytic substance was proved to be identical with surfactin which was potent coltting inhibitor in the thrombin fibrinogen system obtained from culture medium of Bacillus subtilis by Kakinuma, et al.
Supplementary Table 2 from Cancer-Associated Adipose Tissue Promotes Breast Cancer Progression by Paracrine Oncostatin M and Jak/STAT3 Signaling (2023)

&lt;p&gt;F: forward primer, R: reverse primer.&lt;/p&gt;
Structure and expression of rat osteosarcoma (ROS 17/2.8) alkaline phosphatase: product of a single copy gene. (1988)

Alkaline phosphatase [ALP; orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1] is a ubiquitous enzyme of unknown function expressed at high levels in cells of mineralizing tissues. To study the structure, function, and expression of ALP, a full-length cDNA of rat ALP (2415 bases) was isolated from a ROS 17/2.8 osteosarcoma cell lambda gt10 cDNA library. The predicted amino acid sequence spans 524 residues and includes an N-terminal signal peptide of 17 amino acids, the phosphohydrolase active site, a rather hydrophilic backbone with five potential N-glycosylation sites, and a short hydrophobic C-terminal sequence. ALP negative CHO cells transfected with an expression vector containing the ALP coding sequences express ALP. The rat bone, liver, and kidney ALP shows remarkable 90% homology with the corresponding human enzyme, the most divergent region being the C-terminal hydrophobic domain through which the enzyme may be anchored to the plasma membrane. The rat ALP also shows 50% homology with the human placental and intestinal ALP and 25% homology with the Escherichia coli ALP. The amino acids involved in catalysis show nearly complete homology among all known ALP sequences, suggesting that these enzymes evolved from a common ancestral gene. The rat ALP cDNA pRAP 54, used as a hybridization probe in RNA blot analysis of several tissues that express ALP, revealed the presence of an ALP mRNA of approximately equal to 2500 bases. Furthermore, hybridization patterns derived from Southern blot analysis of rat chromosomal DNA offered molecular evidence that the ALP expressed in ROS 17/2.8 osteosarcoma and various rat tissues, excluding the intestine, is the product of the same single copy gene.
Machine learning attacks on 65nm Arbiter PUFs: Accurate modeling poses strict bounds on usability (2012)

Arbiter Physically Unclonable Functions (PUFs) have been proposed as efficient hardware security primitives for generating device-unique authentication responses and cryptographic keys. However, the assumed possibility of modeling their underlying challenge-response behavior causes uncertainty about their actual applicability. In this work, we apply well-known machine learning techniques on challenge-response pairs (CRPs) from 64-stage Arbiter PUFs realized in 65nm CMOS, in order to evaluate the effectiveness of such modeling attacks on a modern silicon implementation. We show that a 90%-accurate model can be built from a training set of merely 500 CRPs, and that 5000 CRPs are sufficient to perfectly model the PUFs. To study the implications of these attacks, there is need for a new methodology to assess the security of PUFs suffering from modeling. We propose such a methodology and apply it to our machine learning results, yielding strict bounds on the usability of Arbiter PUFs. We conclude that plain 64-stage Arbiter PUFs are not secure for challenge-response authentication, and the number of extractable secret key bits is limited to at most 600.
Intranasal insulin improves cognition and modulates β-amyloid in early AD (2007)

The results of this pilot study support further investigation of the benefits of intranasal insulin for patients with Alzheimer disease, and suggest that intranasal peptide administration may be a novel approach to the treatment of neurodegenerative disorders.
Pax3 and Tbx5 Specify Whether PDGFRα+ Cells Assume Skeletal or Cardiac Muscle Fate in Differentiating Embryonic Stem Cells (2014)

Embryonic stem cells (ESCs) represent an ideal model to study how lineage decisions are established during embryonic development. Using a doxycycline-inducible mouse ESC line, we have previously shown that expression of the transcriptional activator Pax3 in early mesodermal cells leads to the robust generation of paraxial mesoderm progenitors that ultimately differentiate into skeletal muscle precursors. Here, we show that the ability of this transcription factor to induce the skeletal myogenic cell fate occurs at the expenses of the cardiac lineage. Our results show that the PDGFRα+FLK1--subfraction represents the main population affected by Pax3, through downregulation of several transcripts encoding for proteins involved in cardiac development. We demonstrate that although Nkx2-5, Tbx5, and Gata4 negatively affect Pax3 skeletal myogenic activity, the cardiac potential of embryoid body-derived cultures is restored solely by forced expression of Tbx5. Taking advantage of this model, we used an unbiased genome-wide approach to identify genes whose expression is rescued by Tbx5, and which could represent important regulators of cardiac development. These findings elucidate mechanisms regulating the commitment of mesodermal cells in the early embryo and identify the Tbx5 cardiac transcriptome.
Adsorption of Water Vapor on Silica, Alumina, and Their Mixed Oxide Aerogels (2001)

SiO2 and Al2O3 aerogels and mixed SiO2−Al2O3 aerogels exhibit excellent adsorption capacities of water vapor in the range above 1.0 kg of water/kg of aerogel. Adsorption properties of mixed SiO2−Al2O3 aerogels remain stable even after 25 repeated adsorption/desorption cycles.
A 3x blind ADC-based CDR (2013)

This paper uses a 3-bit ADC to blindly sample the received data at 3× the baud rate to recover the data. By moving from 2× to 3× sampling, we reduce the required ADC resolution from 5-bit to 3-bit, thereby reducing the overall power consumption by a factor of 2. Measurements from our fabricated test chip in Fujitsu's 65nm CMOS show a high frequency jitter tolerance of 0.19UIpp for a 5Gbps PRBS31 with a 16" FR4 channel.
Lunar regolith penetrators and cutters (1991)

An apparatus was designed and built for conducting simulation experiments on cutting tool penetration in the centrifuge. This equipment is mounted on the laminar container which is used for the regolith densification study, so that the end product of the latter, i.e., a regolith bed with the proper density profile, can be used directly for the penetration tests. In this apparatus, an etching tool is suspended through a pulley system by the action of a double acting air cylinder. By adjusting the air pressure acting on each side of the cylinder, the net downward force acting on the tool can be controlled. The penetration of the tool is measured by an LVDT. This apparatus was proof-tested in the centrifuge and is ready for use in conjunction with the regolith densification experiments.
Dye-Sensitized Solar Cells Based on Three-Dimensional Web-Like Structure Anodes (2012)

TiO2 films with three-dimensional web-like structure have been prepared by the photo polymerization-induced phase separation method (PIPS). Scanning electron microscopy and X-ray diffraction were used to characterize the as-prepared TiO2 films. The results showed that the film texture could be tuned by changing the composition of the precursor solution. The TiO2 film with web-like structure exhibited high photocatalytic activity for the degradation of methylene blue (MB) dye. The as-prepared films were used as the photo-anodes in dye-sensitized solar cells (DSCs). The photoelectric conversion efficiency of the DSCs was significantly enhanced by changing the POGTA/TTB in the precursor solution. Because of the increased dye adsorption active sites and efficient electron transport in the TiO2 anode film, a photoelectric conversion efficiency of 3.015% was obtained.
Paper and Ink Technology Applied for High-speed Inkjet Production Printing (2016)

連帳ロール紙に高速で印字するインクジェット印刷装置はデジタル印刷，すなわちバリアブル，オンデマンドを実現する手段として成長を続けている。印刷物としては低コストの用紙とインクを用いた中程度品質のカラー画像出力であり，少部数かつ宛名もコンテンツも個別対応するという差別化を図ったダイレクトメール，トランザクション，トランスプロモーションなどである。
Data on multimodal approach for early poor outcome (Cerebral Performance Categories 3-5) prediction after cardiac arrest (2018)

The data presented in this article are related to our research article entitled 'Neurophysiological and neuroradiological multimodal approach for early poor outcome prediction after cardiac arrest' (Scarpino et al., 2018) [1]. We reported two additional analyses, including results gathered from somatosensory evoked potentials(SEPs), brain computed tomography(CT) and electroencephalography(EEG) performed on 183 subjects within the first 24 h after cardiac arrest(CA). In the first analysis, we considered the Cerebral Performance Categories(CPC) 3, 4 and 5a,b (severe disability, unresponsive wakefulness state, neurological death and non-neurological death, respectively) as poor outcomes. In the second analysis, patients that died from non-neurological causes (CPC 5b) were excluded from the analysis. Concerning the first analysis, bilateral absent/absent-pathologic(AA/AP) cortical SEPs predicted poor outcome with a sensitivity of 49.3%. A Grey Matter/White Matter(GM/WM) ratio <1.21 predicted poor outcome with a sensitivity of 41.7%. Isoelectric/burst-suppression EEG patterns predicted poor outcome with a sensitivity of 33.5%. If at least one of these poor prognostic patterns was present, the sensitivity for an ominous outcome increased to 60.9%. Concerning the second analysis, AA/AP cortical SEPs predicted poor outcome with a sensitivity of 52.5%. GM/WM ratio <1.21 predicted poor outcome with a sensitivity of 50.4%. Isoelectric/burst-suppression EEG patterns predicted poor outcome with a sensitivity of 39.8%.
HMG-CoA reductase inhibitor ameliorates diabetic nephropathy by its pleiotropic effects in rats (2003)

Statin treatment prevented glomerular injury, independent of the cholesterol-lowering effects. Our findings suggest that the beneficial effect might be mediated by pleiotropic effects including an anti-inflammatory action through a reduction of oxidative stress, NF-kappaB activation, ICAM-1 expression and macrophage infiltration in the early phase of DN.
Riccardo Fubini, Umanesimo e Secolarizzazione da Petrarca a Valla (1992)

Weber Henri. Riccardo Fubini, Umanesimo e Secolarizzazione da Petrarca a Valla. In: Bulletin de l'Association d'étude sur l'humanisme, la réforme et la renaissance, n°34, 1992. pp. 111-114.
Optical Fiber Sensors and Their Applications for Explosive Detection (2012)

This chapter focuses on fiber-optic explosive detection incorporating the use of amplifying fluorescent polymers (AFPs) and describing some detailed work done in our group. It provides a brief review of existing fiber-optic-based explosive detectors. The chapter then discusses high performance fiber-optic explosive detector based on the AFP thin film. For the detection of quenched fluorescence light introduced by AFP thin film, only a few of the hundreds of existing fiber-optic sensing architectures are suitable for the purpose. The chapter describes the process of generating high quality polymer film and pretreating with adhesion promoter. It discusses the effect of photodegradation on AFP polymer. The chapter focuses on optimizing polymer concentration for optimized AFP-film thickness. Finally, it talks about explosive vapor preconcentration and delivery. Controlled Vocabulary Terms fluorescent screens; optical fiber sensors; polymers; thin film devices; vaporisation
Spatial Modulation in Zero-Padded Single Carrier Communication (2012)

In this paper, we consider the Spatial Modulation (SM) system in a frequency selective channel under single carrier (SC) communication scenario and propose zero-padding instead of cyclic prefix considered in the existing literature. We show that the zero-padded single carrier (ZP-SC) SM system offers full multipath diversity under maximum-likelihood (ML) detection, unlike the cyclic prefixed SM system. Further, we show that the order of ML decoding complexity in the proposed ZP-SC SM system is independent of the frame length and depends only on the number of multipath links between the transmitter and the receiver. Thus, we show that the zero-padding in the SC SM system has two fold advantage over cyclic prefixing: 1) gives full multipath diversity, and 2) offers relatively low ML decoding complexity. Furthermore, we extend the partial interference cancellation receiver (PIC-R) proposed by Guo and Xia for the decoding of STBCs in order to convert the ZP-SC system into a set of flat-fading subsystems. We show that the transmission of any full rank STBC over these subsystems achieves full transmit, receive as well as multipath diversity under PIC-R. With the aid of this extended PIC-R, we show that the ZP-SC SM system achieves receive and multipath diversity with a decoding complexity same as that of the SM system in flat-fading scenario.
Vertically stacked gate-all-around Si nanowire transistors: Key Process Optimizations and Ring Oscillator Demonstration (2017)

We report on CMOS-integrated vertically stacked gate-all-around (GAA) Si nanowire (NW) MOSFETs with in-situ doped source-drain stressors and dual work function metal gates. We demonstrate that oxidation-induced SiGe/Si fin deformation by STI densification is effectively suppressed by a SiN liner. This SiN fin protection improves the controllability of nanowire formation. In addition, highly-selective Si nano-wire release and inner spacer cavity formation without Si re-flow are demonstrated. Finally, for the first time we report functional ring oscillators based on stacked Si NW-FETs.
P-212 (2012)

Clark, Kathleen A; Chanda, Debjani; Balte, Pallavi; Cai, Bo; Vena, John; Karmaus, Wilfried; Svendsen, Erik R Author Information