gebhart/patrepeval huggingface dataset:
Expression of a protein tyrosine phosphatase in normal and v-src-transformed mouse 3T3 fibroblasts (1992)

A rat cDNA encoding a 51-kD protein tyrosine phosphatase (PTP1) was cloned into a mammalian expression vector and transfected into normal and v-src-transformed mouse NIH 3T3 fibroblasts. In the stable subclones isolated, PTP1 expression at the mRNA level was elevated twofold to 25-fold. The highest constitutive level of phosphotyrosine-specific dephosphorylating activity observed without cytotoxic effects or significant clonal instability was approximately 10-fold over the endogenous activity. The expressed PTP1 was found to be associated with the particulate fraction of the fibroblasts. Subcellular fractionation and immunofluorescent microscopic examination of PTP1-overexpressing cells has shown the phosphatase to be localized to the reticular network of the ER. PTP1 was readily solubilized by detergents, but not by high salt. Limited proteolysis of membrane-associated PTP1 resulted in the release of lower molecular mass (48 and 37 kD) forms of the enzyme to the cytosol. Thermal phase partitioning of isolated membranes with Triton X-114 indicated that the full-length PTP1 was strongly integrated into the membrane in contrast to the proteolytically derived fragments of PTP1. Overexpression of PTP1 caused little apparent change in the rate of cell proliferation, but did induce changes in fibroblast morphology. A substantial increase in the proportion of bi- and multinucleate cells in PTP1-expressing cell populations was observed, and, in the case of the v-src-transformed cells, cell flattening and loss of refractibility occurred. Although no apparent difference in the tyrosine phosphorylation of pp60v-src was noted in v-src-transformed control and PTP1-overexpressing fibroblasts, the phosphotyrosine content of a 70-kD polypeptide was decreased in PTP1-overexpressing cells.
Preparation, structure and analysis of the bonding in the molecular entity (OSO)2Li{[AlF(ORF)3]Li[Al(ORF)4]} (RF = C(CF3)3) (2010)

The (SO(2))(2)Li[AlF(OR(F))(3)]Li[Al(OR(F))(4)] (1) (R(F) = C(CF(3))(3)) molecular entity was obtained by thermal decomposition of Li[Al(OR(F))(4)] followed by crystallization from liquid SO(2). 1, containing two SO(2) molecules eta(1)-O coordinated to Li(+), was structurally characterized by single crystal X-ray diffraction and NMR spectroscopy in SO(2)(l). Bonding analyses of 1 (bond valency units, AIM analysis, atomic charges, bond orders) show that 1 can be either considered as a Li(OSO)(2)(+) complex stabilized by the large WCA [AlF(OR(F))(3)](-)Li(+)[Al(OR(F))(4)](-) or as consisting of 2 SO(2), 2 Li(+), [AlF(OR(F))(3)](-), and [Al(OR(F))(4)](-) joined by electrostatic interactions into the discrete molecular entity 1. The bonding between Li(+) and SO(2) molecules is shown to be almost completely attributable to monopole-induced dipole electrostatic interactions. Theoretical gas phase lithium ion affinity of SO(2) is determined to be stronger than its silver(I) ion affinity owing largely to the shorter lithium SO(2) contacts in the calculated structures that increase the electrostatic interaction.
Design of an 874 GHz biasable sub-harmonic mixer based on MMIC membrane planar schottky diodes (2008)

We report upon the development of a 874 GHz bias-able sub-harmonic mixer using planar Schottky diodes. The MMIC integrates two planar Schottky diodes in a balanced configuration, stripline filtering elements and on-chip capacitor on a 5 microns thick GaAs membrane. Predicted performance gives optimal conversion losses of 10 dB and a 3 dB bandwidth from 820 to 920 GHz. This work represents the highest frequency Schottky based MMIC sub-harmonic mixer development to-date.
Additive effect of tolC and rfa mutations on the hydrophobic barrier of the outer membrane of Escherichia coli K-12 (1994)

Studies using tolC mutant derivatives of deep rough (rfa) mutants indicate that tolC and rfa mutations have an additive effect with respect to their sensitivity to hydrophobic agents, suggesting that they are not acting through a mutual mechanism to alter the permeability of the outer membrane.
Predictors of Lung Cancer among Asbestos-exposed Men in the -Carotene and Retinol Efficacy Trial (2005)

Despite numerous published studies, debate continues regarding the risk of developing lung cancer among men exposed occupationally to asbestos, particularly those without radiographic or functional evidence of asbestosis. The beta-Carotene and Retinol Efficacy Trial (CARET), a study of vitamin supplementation for chemoprevention of lung cancer, has followed 4,060 heavily exposed US men for 9-17 years. Lung cancer incidence for 1989-2002 was analyzed using a stratified proportional hazards model. The study confirmed excessive rates of lung cancer among men with radiographic asbestosis. Comparison of study arms revealed a strong, unanticipated synergy between radiographic profusion category and the active intervention. In the large subgroup of men with normal lung parenchyma on chest radiograph at baseline, there was evidence of exposure-related lung cancer risk: Men with more than 40 years' exposure in high-risk trades had a risk approximately fivefold higher than men with 5-10 years, after adjustment for covariates. The effect in these men was independent of study intervention arm, but pleural plaques on the baseline radiograph and abnormal baseline flow rate were strong independent predictors of subsequent lung cancer. Residual confounding by subclinical asbestosis, exposure to unmeasured lung carcinogens, or differences in smoking are unlikely to explain these observations better than a carcinogenic effect of asbestos per se.
Recent advances in Yb 3+ -doped silica fiber lasers (1994)

Yb3+-doped germanosilicate fiber has been demonstrated to be a versatile host for laser action at wavelengths from 1 to 1.2 μm.1 It offers many very attractive features including broad absorption (800-1064 nm) and emission (970-1200 nm) bands. The pump band includes AlGaAs and InGaAs diode wavelengths as well as Nd:YLF and Nd:YAG wavelengths where very high powers are available. The emission band includes wavelengths of interest for specific applications, e.g., a 1020-nm source for pumping of 1.3 pm Pr3+-doped fiber amplifiers and upconversion lasers, a source at 1140 nm for pumping of Tm3+-doped fluoride fiber blue upconversion lasers, and singlefrequency sources for spectroscopic applications.
T Helper Cell Type 2 Cytokines Coordinately Regulate Immunoglobulin E–Dependent Cysteinyl Leukotriene Production by Human Cord Blood–Derived Mast Cells (2001)

Human mast cells (hMCs) derived in vitro from cord blood mononuclear cells exhibit stem cell factor (SCF)-dependent comitogenic responses to T helper cell type 2 (Th2) cytokines. As cysteinyl leukotriene (cys-LT) biosynthesis is a characteristic of immunoglobulin (Ig)E-activated mucosal hMCs, we speculated that Th2 cytokines might regulate eicosanoid generation by hMCs. After passive sensitization for 5 d with IgE in the presence of SCF, anti-IgE-stimulated hMCs elaborated minimal cys-LT (0.1 +/- 0.1 ng/10(6) hMCs) and abundant prostaglandin (PG)D(2) (16.2 +/- 10.3 ng/10(6) hMCs). Priming of hMCs by interleukin (IL)-4 with SCF during passive sensitization enhanced their anti-IgE-dependent histamine exocytosis and increased their generation of both cys-LT (by 27-fold) and PGD(2) (by 2. 5-fold). Although priming with IL-3 or IL-5 alone for 5 d with SCF minimally enhanced anti-IgE-mediated cys-LT generation, these cytokines induced further six- and fourfold increases, respectively, in IgE-dependent cys-LT generation when provided with IL-4 and SCF; this occurred without changes in PGD(2) generation or histamine exocytosis relative to hMCs primed with IL-4 alone. None of these cytokines, either alone or in combination, substantially altered the levels of cytosolic phospholipase A(2) (cPLA(2)), 5-lipoxygenase (5-LO), or 5-LO activating protein (FLAP) protein expression by hMCs. In contrast, IL-4 priming dramatically induced the steady-state expression of leukotriene C(4) synthase (LTC(4)S) mRNA within 6 h, and increased the expression of LTC(4)S protein and functional activity in a dose- and time-dependent manner, with plateaus at 10 ng/ml and 5 d, respectively. Priming by either IL-3 or IL-5, with or without IL-4, supported the localization of 5-LO to the nucleus of hMCs. Thus, different Th2-derived cytokines target distinct steps in the 5-LO/LTC(4)S biosynthetic pathway (induction of LTC(4)S expression and nuclear import of 5-LO, respectively), each of which is necessary for a full integrated functional response to IgE-dependent activation, thus modulating the effector phenotype of mature hMCs.
Assisted satellite positioning (2005)

Although standard satellite positioning provides unbeatable performance, compared with other available positioning methods it still suffers from many environment-related issues. One of them is disruption of continuity of positioning service, usually caused by temporal inability to collect navigation data due to poor satellite visibility. This problem is particularly noticeable in urban areas, where high buildings often considerably decrease the satellite visibility. A number of GPS-based systems and services (among them the location-based services - LBS) rely upon continuous provision of satellite positioning service. In order to decrease as much as possible the time to first fix (TTFF) after temporal unavailability of standard positioning service, auxiliary communications systems deploy the functionality of assistance data provision to satellite positioning receiver. This article examines the content of satellite positioning assistance, the impact of assistance data on position estimate calculation algorithm and the means for assistance data distribution. Special emphasis is given on satellite positioning assistance for LBS, and the mechanisms for assistance data delivery through the mobile communication network are presented. In conclusion, assistance data are identified as the major contributor to satellite positioning performance improvement, with a brief list of related future activities given
Particulate matter air pollution causes oxidant-mediated increase in gut permeability in mice (2011)

Exposure to high doses of urban PM causes oxidant dependent GI epithelial cell death, disruption of tight junction proteins, inflammation and increased permeability in the gut in vitro and in vivo. These PM-induced changes may contribute to exacerbations of inflammatory disorders of the gut.
Altered expression of hepatic carcinogen metabolizing enzymes with liver injury in HBV transgenic mouse lineages expressing various amounts of hepatitis B surface antigen (1999)

These results suggest that liver cell injury induced by accumulation of HBV antigens can result in the induction of some carcinogen metabolizing enzymes and this may be one mechanism of chemical-viral interaction in hepatocarcinogenesis.
Utilização de uma água residuária da extrusão de alumínio na neutralização de efluentes ácidos da mineração de carvão. (2002)

Efluentes Ácidos gerados pela mineração do carvão (EDA) requerem a neutralização antes de serem liberados para o meio ambiente. Para este fim, várias substâncias alcalinas podem ser utilizadas, tais como cal (CaO) e calcário (CaCO3). A adição da solução alcalina promove a elevação do pH do efluente ácido e também a precipitação de muitos metais presentes em solução. Este trabalho apresenta e discute a viabilidade técnica de se utilizar um efluente alcalino (ESU) gerado na indústria de perfis de alumínio (Alcoa Tubarão - SC) na neutralização de efluentes de drenagem ácida existentes na região sul de Santa Catarina. Ensaios em laboratório (titulações) complementados por testes industriais foram realizados objetivando determinar o consumo do ESU necessário para elevar o pH do EDA de 2,5 para 6,0 - 6,5. Os resultados indicaram que 1 m³ do ESU é empregado para neutralizar 1300 m³ de EDA. Esta proporção volumétrica vem sendo empregada em escala industrial e consentida pelo Órgão Ambiental de Santa Catarina (FATMA). A análise da composição química do EDA após ter sido neutralizado pelo ESU mostrou que a concentração dos metais pesados presentes em solução está em concordância com os limites legais estabelecidos pela FATMA, exceto para a concentração do manganês (3,08 mg/L). Este problema pode ser explicado pela alta solubilidade do hidróxido de manganês na faixa de pH entre 6,0-6,5 (aplicação industrial).
Role of CRF Receptor Signaling in Stress Vulnerability, Anxiety, and Depression (2009)

Markers of hyperactive central corticotropin releasing factor (CRF) systems and CRF-related single nucleotide polymorphisms (SNPs) have been identified in patients with anxiety and depressive disorders. Designing more effective antagonists may now be guided by data showing that small molecules bind to transmembrane domains. Specifically, CRF(1) receptor antagonists have been developed as novel anxiolytic and antidepressant treatments. Because CRF(1) receptors become rapidly desensitized by G protein-coupled receptor kinase (GRK) and beta-arrestin mechanisms in the presence of high agonist concentrations, neuronal hypersecretion of synaptic CRF alone may be insufficient to account for excessive central CRF neurotransmission in stress-induced affective pathophysiology. In addition to desensitizing receptor function, GRK phosphorylation and beta-arrestin binding can shift a G protein-coupled receptor (GPCR) to signal selectively via the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK-MAPK) or Akt pathways independent of G proteins. Also, Epac-dependent CRF(1) receptor signaling via the ERK-MAPK pathway has been found to potentiate brain-derived neurotrophic factor (BDNF)-stimulated TrkB signaling. Thus, genetic or acquired abnormalities in GRK and beta-arrestin function may be involved in the pathophysiology of stress-induced anxiety and depression.
On viewing distance and visual quality assessment in the age of Ultra High Definition TV (2014)

International audience
Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae (1992)

Yeast RNA polymerase II initiation factors a, b, e, and g were isolated from whole cell extract and found to be sufficient, when combined with bacterially expressed yeast transcription factor (TF) IID, to enable RNA polymerase II to utilize nine different eukaryotic promoters in vitro, and to initiate transcription at sites used in vivo. The purified factors did not contain the previously described transcription factor IIA (TFIIA). TFIIA failed to substitute for any purified factor or to stimulate transcription with the complete set of factors, indicating that its function in crude extracts is primarily as an anti-inhibitor.
Erratum: Nonlinear Observer Design in the Siegel Domain (2004)

There is an error in the proof of the main result of our paper SIAM J. Control Optim., 41 (2002), pp. 932--953]. An additional assumption is needed for the main result to hold. In this erratum, we supply a corrected version of the main result.
Adaptive power amplifiers for military and commercial communications (2006)

Architectures are discussed in which power amplifiers are able to vary their performance characteristics in response to changing output power levels, frequencies, load impedance levels and linearity constraints. Necessary elements for implementation of such systems are reviewed, including sensors to detect changes in the external environment, actuators to adapt the amplifier characteristics, and algorithms to implement the associated control functions. An example is described of a power amplifier for cell phone applications that measures the load impedance provided by the antenna and varies its output impedance match accordingly, in order to preserve output power, efficiency and linearity as the external antenna is handled. The system automatically provides output tuning for a 1W linear amplifier, and can accommodate output standing wave ratios up to 8:1. It improves output power and efficiency by x2 in representative mismatch scenarios. The insertion loss of the system is comfortably low at 0.5dB.
Heregulin: A new shelterin component at human telomeres (2006)

4995 Telomere length, shape and function depend on a six subunits protein complex (TRF1, TRF2, TIN2, hRap1, TPP1 and POT1) recently referred as shelterin. We here describe a novel human telomere-associated protein, the tumor-promoting growth factor Heregulin (HRG). Utilizing the yeast two-hybrid system to identify HRG-interacting proteins in a human mammary gland cDNA library, a reactive clone was found to encode hRap1, a known association factor of TRF2. DNA sequencing and deletion mapping revealed that the hRap1’s linker domain, a region previously suggested recruiting negative regulators of telomere length, was essential for interaction between hRap1 and HRG. Double immunofluorescence microscopy revealed that the transfected green fluorescence protein (GFP)-tagged HRG and the endogenous HRG both co-localized not only with hRap1 but also with the hRap1’s partner TRF2. This interaction was reproduced in vivo in human cancer cells as immunoprecipitation of endogenous HRG from cell nuclei extracts was able to specifically pull down TRF2. Forced overexpression of HRG in HRG-negative cancer cells increased the rate of telomere shortening and resulted in increased levels of hRap1 and TRF2. Treatment of HRG-overexpressing cancer cells with HRG small interfering RNA led to telomere elongation and resulted in a decreased presence of hRap1 and TRF2. Our findings propose HRG as a novel shelterin team member that, by interplaying with hRap1 and TRF2, negatively regulates telomere length in human cancer cells.
Plateau modulus of epoxidized polybutadiene (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPlateau modulus of epoxidized polybutadieneC. M. Roland, J. K. Kallitsis, and K. G. GravalosCite this: Macromolecules 1993, 26, 24, 6474–6476Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/ma00076a025https://doi.org/10.1021/ma00076a025research-articleACS PublicationsRequest reuse permissionsArticle Views195Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Association between posterior uveal melanoma and iris freckles, iris naevi, and choroidal naevi (2003)

This study did not detect an association between posterior uveal melanoma and iris freckles, iris naevi, or choroidal naevi.
Plasmonics: A Spoof Surface Plasmon Transmission Line Loaded with Varactors and Short‐Circuit Stubs and Its Application in Wilkinson Power Dividers (Adv. Mater. Technol. 6/2018) (2018)

In article number 1800046, Tie Jun Cui and co-workers propose a tunable spoof surface plasmon transmission line and construct a Wilkinson power divider based on it. It provides a versatile way to transform passive devices composed of microstrip lines into compact and frequency-reconfigurable ones.
Long-term maintenance of human naïve T cells through in situ homeostasis in lymphoid tissue sites (2016)

Naïve T cells develop in the thymus and coordinate immune responses to new antigens; however, mechanisms for their long-term persistence over the human lifespan remain undefined. Here, we investigated human naïve T cell development and maintenance in primary and secondary lymphoid tissues obtained from individual organ donors aged 3 months-73 years. In the thymus, the frequency of double-positive thymocytes declined sharply in donors over age 40 coincident with reduced recent thymic emigrants (RTE) in lymphoid tissues, while naïve T cells were functionally maintained predominantly in lymph nodes (LN). Analysis of TCR clonal distribution by CDR3 sequencing of naïve CD4<sup>+</sup> and CD8<sup>+</sup> T cells in spleen and LNs reveal site-specific clonal expansions of naïve T cells from individuals >40 years of age with minimal clonal overlap between lymphoid tissues. We also identified biased naïve T cell clonal distribution within specific lymph nodes based on VJ usage. Together these results suggest prolonged maintenance of naïve T cells through <i>in situ</i> homeostasis and retention in lymphoid tissue.
Time Dependence of Anode Spot Formation Threshold Current in Vacuum Arcs (1978)

The variation of threshold current for the transition between the low current quiescent vacuum arc mode, and the high voltage noisy mode associated with anode spot formation, was measured as a function of peak current, current waveform frequency, and electrode separation on fixed diameter (25 mm) Cu and Ni electrodes. At current waveform frequencies of about 60 Hz on Cu electrodes, the threshold current depends mainly on electrode spacing, as has been observed by other investigators. However, at higher waveform frequencies, the threshold current becomes a strong function of peak current as well. At 347 Hz on 25 mm. diam. Cu electrodes separated by 10 mm, the threshold current rose from approximately 2 kA to 5.5 kA, as the peak current rose from 2 kA to 6 kA. At 543 Hz on 25 mm diam Ni electrodes separated by 9 mm, a saturation in threshold current at about 7.5 kA was observed for peak currents greater than 9 kA. Simultaneous anode temperature measurements indicated that the Ni anode surface temperature immediately prior to transition rose from about 1550° K to 2250° K with variations of peak current from 5 kA to 13 kA. Predictions of the variation of threshold current based on random transitions, and on cathode spot migration over the edge of the cathode, are compared with the experimental data.
Role de L'Oxygene et Effets des Eaux Residuaires Sur La Survie D'Escherichia Coli en Mer Role of Oxygen and Effects of Sewage on Survival of Escherichia Coli in Seawater (1996)

The decrease in the viability of Escherichia coli cells in seawater results from the stress induced by seawater itself as well as their story before their release into the marine environment. Viability in seawater of E. coli cells previously grown anaerobically was after 2 days of starvation in seawater 8.5 times lower than that of cells previously grown aerobically and was 70 times lower for cells starved 6 days in seawater. A previous suspension of cells in sewage decreased 3 to 9 times their subsequent viability in seawater after 2 days of starvation in seawater but viability was no more affected after 6 days of starvation in seawater.