MP28-19 GENOME-WIDE ANALYSIS OF COPY NUMBER ALTERATIONS IN RENAL-PELVIC AND URETERAL CANCERS (2014)

You have accessJournal of UrologyBladder Cancer: Basic Research II1 Apr 2014MP28-19 GENOME-WIDE ANALYSIS OF COPY NUMBER ALTERATIONS IN RENAL-PELVIC AND URETERAL CANCERS Shigekatsu Maekawa, Taketo Kawai, Yusuke Sato, Toru Nakagawa, Haruki Kume, Seishi Ogawa, and Yukio Homma Shigekatsu MaekawaShigekatsu Maekawa More articles by this author , Taketo KawaiTaketo Kawai More articles by this author , Yusuke SatoYusuke Sato More articles by this author , Toru NakagawaToru Nakagawa More articles by this author , Haruki KumeHaruki Kume More articles by this author , Seishi OgawaSeishi Ogawa More articles by this author , and Yukio HommaYukio Homma More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.668AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Accounting for about 10% of all urinary tract cancers, pathogenesis of upper urinary tract cancers have been poorly understood, especially in terms of the spectrum of genetic lesions. We investigated a genome-wide analysis of copy number alterations. METHODS We extracted DNA from frozen surgical specimens from a total of 68 patients with renal-pelvic and ureteral cancers who underwent nephroureterectomy at our institute. The samples were analyzed for alterations in genomic copy numbers as well as loss of heterozygosity (LOH) using Affymetrix 250K NspI arrays. RESULTS The patients were comprised of 78 males and 31 females with median age of 66 years old (43 to 85). Pathological T stages were: 3 in pTis, 48 (44%) in non-invasive cancer (pTa and pT1), 57 (52.8%) in invasive cancer (pT2, pT3 and pT4). While Grade1 and 2 tumors were 57 (52.8%), Grade3 tumors were 51 (47.2%). We found frequent allelic imbalances at 8p, 9p and17p; 9p LOH was the most common abnormality (44%) followed by 17p amplification (39%) and 3p amplification (27%). Moreover, frequency of 17p amplification is lower in invasive cancer (p=0.001) or Grade3 cancer (0.017) than in non-invasive cancer or Grade1, 2 cancer. AND 1p deletion and 5p gain are related to cause specific survival (p=0.005, p=0.008). In addition, we detected the regions which may be related to develop renal-pelvic and ureteral cancers (p<1*10-10); 1q23, 3p25, 8p12, 8q22, 9p21, 10p14, 11q13, 15q11, 17q11, 17p12, 17q25, 18p11, 19q12, 20q13, 21q22, 22q11. CONCLUSIONS Our results were similar to those previously reported in bladder cancers. Our genome-wide study confirmed and integrated sporadically-reported mechanisms of molecular pathogenesis of urothelial cancers. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e303-e304 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Shigekatsu Maekawa More articles by this author Taketo Kawai More articles by this author Yusuke Sato More articles by this author Toru Nakagawa More articles by this author Haruki Kume More articles by this author Seishi Ogawa More articles by this author Yukio Homma More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...