gebhart/patrepeval huggingface dataset:
A dedicated flavin-dependent monooxygenase catalyzes the hydroxylation of demethoxyubiquinone into ubiquinone (coenzyme Q) in Arabidopsis (2021)

Ubiquinone (Coenzyme Q) is a vital respiratory cofactor and liposoluble antioxidant. In plants, it is not known how the C-6 hydroxylation of demethoxyubiquinone, the penultimate step in ubiquinone biosynthesis, is catalyzed. The combination of cross-species gene network modeling along with mining of embryo-defective mutant databases of Arabidopsis thaliana identified the embryo lethal locus EMB2421 (At1g24340) as a top candidate for the missing plant demethoxyubiquinone hydroxylase. In marked contrast with prototypical eukaryotic demethoxyubiquinone hydroxylases, the catalytic mechanism of which depends on a carboxylate-bridged di-iron domain, At1g24340 is homologous to FAD-dependent oxidoreductases that instead use NAD(P)H as an electron donor. Complementation assays in Saccharomyces cerevisiae and Escherichia coli demonstrated that At1g24340 encodes a functional demethoxyubiquinone hydroxylase and that the enzyme displays strict specificity for the C-6 position of the benzoquinone ring. Laser-scanning confocal microscopy also showed that GFP-tagged At1g24340 is targeted to mitochondria. Silencing of At1g24340 resulted in 40 to 74% decrease in ubiquinone content and de novo ubiquinone biosynthesis. Consistent with the role of At1g24340 as a benzenoid ring modification enzyme, this metabolic blockage could not be bypassed by supplementation with 4-hydroxybenzoate, the immediate precursor of ubiquinone's ring. Unlike in yeast, in Arabidopsis overexpression of demethoxyubiquinone hydroxylase did not boost ubiquinone content. Phylogenetic reconstructions indicated that plant demethoxyubiquinone hydroxylase is most closely related to prokaryotic monooxygenases that act on halogenated aromatics and likely descends from an event of horizontal gene transfer between a green alga and a bacterium.
ChemInform Abstract: HALOMETHYL‐METAL COMPOUNDS PART 77, AN ORGANOMERCURY ROUTE TO TETRAFLUOROETHYLIDENE (1975)

Abstract Phenyl‐( 1‐brom‐1 ,2,2,2‐tetrafluoräthyl)‐quecksilber (IV), dargestellt aus Phenylquecksilberchlorid (I) mit dem Tetrafluorbromäthan (II) und Natriummethylat (III), reagiert mit den cyclischen Olefinen (V) zu den Bicyclen (VI) und (VII).
Transcriptional regulation of the novobiocin biosynthetic gene cluster (2009)

The aminocoumarin antibiotic novobiocin is a gyrase inhibitor formed by a Streptomyces strain. The biosynthetic gene cluster of novobiocin spans 23.4 kb and contains 20 coding sequences, among them the two regulatory genes novE and novG. We investigated the location of transcriptional promoters within this cluster by insertion of transcriptional terminator cassettes and RT-PCR analysis of the resulting mutants. The cluster was found to contain eight DNA regions with promoter activity. The regulatory protein NovG binds to a previously identified binding site within the promoter region located upstream of novH, but apparently not to any of the other seven promoters. Quantitative real-time PCR was used to compare the number of transcripts in a strain carrying an intact novobiocin cluster with strains carrying mutated clusters. Both in-frame deletion of the regulatory gene novG and insertion of a terminator cassette into the biosynthetic gene novH led to a strong reduction of the number of transcripts of the genes located between novH and novW. This suggested that these 16 biosynthetic genes form a single operon. Three internal promoters are located within this operon but appear to be of minor importance, if any, under our experimental conditions. Transcription of novG was found to depend on the presence of NovE, suggesting that the two regulatory genes, novE and novG, act in a cascade-like mechanism. The resistance gene gyrB(R), encoding an aminocoumarin-resistant gyrase B subunit, may initially be co-transcribed with the genes from novH to novW. However, when the gyrase inhibitor novobiocin accumulates in the cultures, gyrB(R) is transcribed from its own promoter. Previous work has suggested that this promoter is controlled by the superhelical density of chromosomal DNA.
An Array Filtering Implementation of a Constant-Beam-Width Acoustic Source (1990)

For acoustic arrays (microphones or loudspeakers) the main beam width of the radiation pattern is determined by the spacing between the elements in terms of the wavelength. Thus the beam pattern depends on the frequency. It is demonstrated that by controlling the spacing and the frequency response of a pair of superposed arrays, constant directivity of the main beam lobe over a wide frequency range can be achieved. Large powers can be achieved with relatively low energy densities at each element
Data from A Urokinase Receptor–Derived Peptide Inhibiting VEGF-Dependent Directional Migration and Vascular Sprouting (2023)

&lt;div&gt;Abstract&lt;p&gt;The receptor for the urokinase-type plasminogen activator (uPAR) is a widely recognized master regulator of cell migration, and uPAR&lt;sub&gt;88–92&lt;/sub&gt; is the minimal sequence required to induce cell motility. We previously showed that soluble forms of uPAR elicit angiogenic responses through their uPAR&lt;sub&gt;88–92&lt;/sub&gt; chemotactic sequence and that the synthetic peptide SRSRY exerts similar effects. By a drug design approach, based on the conformational analysis of the uPAR&lt;sub&gt;88–92&lt;/sub&gt; sequence, we developed peptides (pERERY, RERY, and RERF) that potently inhibit signaling triggered by uPAR&lt;sub&gt;88–92&lt;/sub&gt;. In this study, we present evidence that these peptides are endowed also with a clear-cut antiangiogenic activity, although to different extents. The most active, RERF, prevents tube formation by human endothelial cells exposed to SRSRY. RERF also inhibits VEGF-triggered endothelial cell migration and cord-like formation in a dose-dependent manner, starting in the femtomolar range. RERF prevents F-actin polymerization, recruitment of αvβ3 integrin at focal adhesions, and αvβ3/VEGFR2 complex formation in endothelial cells exposed to VEGF. At molecular level, the inhibitory effect of RERF on VEGF signaling is shown by the decreased amount of phospho-FAK and phospho-Akt in VEGF-treated cells. &lt;i&gt;In vivo&lt;/i&gt;, RERF prevents VEGF-dependent capillary sprouts originating from the host vessels that invaded angioreactors implanted in mice and neovascularization induced by subcorneal implantation of pellets containing VEGF in rabbits. Consistently, RERF reduced the growth and vascularization rate of tumors formed by HT1080 cells injected subcutaneously in the flanks of nude mice, indicating that RERF is a promising therapeutic agent for the control of diseases fuelled by excessive angiogenesis such as cancer. &lt;i&gt;Mol Cancer Ther; 12(10); 1981–93. ©2013 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Failure of Estrogen To Block Compensatory Ovarian Hypertrophy in Prepuberal Rats Bearing Medial Basal Hypothalamic FSH Implants (1970)

Hemiovariectomized rats 30–31 days of age were implanted with FSH in the medial basal hypothalamic region (MBH) or the adenohypophysis, and the hemicastrated controls received LH, LtH or cocoa butter in the MBH. They then were injected sc daily for 10 days with estradiol benzoate (Eb) 0.1 μg, or with oil. At the 11th day post implantation in rats bearing MBH-LtH or LH implants or no implant, Eb treatment prevented the compensatory ovarian hypertrophy (COH) normally observed in oil-treated controls (p <0.01). Eb-treated rats with an MBH-FSH implant showed COH similar to oil-treated controls. Rats with intrapituitary FSH and Eb treatment had significant COH reduction as compared to MBH-FSH-Eb rats (p <0.01). Some rats receiving ip 25 μCi of 17β- 6,7-3H-estradiol the 11th day post implantation were killed 1 hr later, and the hypothalamic uptake of 3H-estradiol was determined. Animals bearing MBH-FSH implants had significantly lower 3H-estradiol uptake than hemiovariectomized rats without implants or with MBH-cocoa butter implants (p <0.01). It seems that FSH in immature rats lacks a negative feedback effect for its own regulation. The results support our concept that FSH has a positive feedback action in the female rat and suggest that FSH may compete with estrogen for the same hypothalamic receptor. (Endocrinology86: 50, 1970)
Heritability and genetic diversity of iron, zinc and some morphological and physiological traits in some spring wheat genotypes (Triticum aestivum L.) (2014)

Wheat is one of the most important cereals and main source of food in Iran and many different countries. Thirty spring common wheat genotypes from Iran were assessed for grain concentrations of Iron and Zinc and some morphological and physiological traits in 2009 and 2010. Iron showed large variation among genotypes, ranging from 17.5 µg g -1 to 48.68 µg g -1 (mean 30.79 µg g -1 ) in 2009 and from 22.74 µg g -1 to 45.60 µg g -1 (mean 30.53 µg g-1) in 2010. Similarly, Zinc concentration varied among genotypes, ranging from 22.36 µg g-1 to 52.69 µg g-1 (mean 35.79 µg g -1 ) in 2009 and from 20.74 µg g -1 to 78.60 µg g -1 (mean 42.45 µg g -1 ) in 2010. The highest heritability was exhibited by days to 50% heading (0.96) in 2009 and by stem number (0.96), spike length (0.96) in 2010. Heritability of grain iron and zinc were 0.74 and 0.61 in 2009, 0.85 and 0.92 in 2010 respectively. Four clusters formed through cluster analysis. Sistan, Akbari, Roshan and Roshan Bahareh backcross genotypes are recommended to use in multiple crossing program to reach transgressive segregants with high genetic potential of studied traits especially for chlorophyll content, grain iron and grain zinc all together. The most squared Euclidean genetic distance (75.81) observed between Niknejad and Roshan genotypes. Therefore, these genotypes can be used to plan wide crosses, to reach genetic diversity and maximize expression of hetrosis. Mahdavi and Bahar genotypes had the most squared Euclidean genetic distance (556.45) for grain iron and zinc content.
A Simple Preparation for Some Hydroxyphenothiazines1 (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Simple Preparation for Some Hydroxyphenothiazines1Edward A. Nodiff and Martin HausmanCite this: J. Org. Chem. 1966, 31, 2, 625–626Publication Date (Print):February 1, 1966Publication History Published online1 May 2002Published inissue 1 February 1966https://pubs.acs.org/doi/10.1021/jo01340a526https://doi.org/10.1021/jo01340a526research-articleACS PublicationsRequest reuse permissionsArticle Views199Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Reducing the complexity of application deployment in large data centers (2005)

The deployment and configuration of distributed applications is a human intensive and highly complex process that poses significant challenges to data center operators. The process involves many cross-cutting concerns such as connectivity, performance, and security requirements, as well as resource availability, policies and best practices. These interdependencies represent a significant source of complexity, cost, and risk in data center management. In this paper we address this problem using a new approach that leverages concepts from the model-driven architecture research domain. We describe a prototype application deployment automation system based on model transformation techniques. We show how model transformation techniques can replace the manual process of writing and adapting scripts and workflows, reduce the deployment complexity, guarantee configuration integrity and consistency, and allow for a separation of concerns.
Deblending with weak signal preserved by dip vector-median filter (2017)

We propose a Dip Vector-Median Filter (D-VMF) by adding the dip field constraint to the Vector-Median Filter in order to deblend simultaneous source data. The dip is provided by plane-wave destruction (PWD) algorithm, which is suitable for the weak signals in the deep. Thus, the D-VMF can filter out the noise while protecting the deep weak signals. Three different median filters, Scalar-Median Filter (SMF), Multi-Directional Vector-Median Filter (MDVMF) and D-VMF, are applied to two synthetic blended data sets. The test results show that our method performs better in both efficiency and accuracy. Presentation Date: Tuesday, September 26, 2017 Start Time: 3:55 PM Location: Exhibit Hall C, E-P Station 2 Presentation Type: EPOSTER
Augmented reality system with planar homographies using building façade (2017)

In recent years, there has been widespread adoption of AR technology with various applications in the urban environment.The essence of an AR application includes the estimation of camera and object placement, which is still a challenging research problem.The first issue is that the structure of the real-world environment is usually very cluttered and involves many unforeseen uncertainties which cannot be generalised as a model.Secondly, the reprojection of the real-world scene to the camera's viewpoint flattens 3D information down to a pixel level, which causes what is known as perspective projection.Fortunately, it is possible to recover the pixel level of the camera pose via planar homography.Methods commonly used in typical AR applications to recover the homography are based on feature-point matching, but many of them still suffer from the problem of perspective and camera movement.By taking characteristics of the urban environment, such as buildings which are widely seen on the street view, we propose a simple, yet efficient, approach to recover the camera pose from a single image.Our approach exploits the building faade of rectilinear structures portrayed by man-made structures (e.g.windows and brick structure).Using lines from these structures, our proposed algorithm can produce a rectified homography for each detected building faade, so we can then recover the fronto-parallel view of each building faade.Since these planar homographies are invertible, we can use them to recover the camera pose of the building faade for the object emplacement task.Finally, we implemented a tracker-based AR application that uses the idea of a fronto-parallel view that results in better matching effectiveness.
A new long pulsed 940 nm diode laser used for hair removal in Asian skin types (2003)

BACKGROUND: The theory of selective photothermolysis has led to the development of a variety of different hair removal laser systems.1,2 These lasers use follicular melanin as a target chromophore. At present the delivery of adequate fluences, optimizing wavelengths and pulse duration, and adequate epidermal cooling are the factors required to achieve effective hair removal. To further evaluate these factors a study was initiated to further investigate a new laser system.
Taurine distribution in different cat muscles as visualized by immunohistochemistry: changes with stimulus state. (1993)

The distribution of endogenous taurine in the cardiac pectinate muscle, the intestinal wall smooth muscle and in nine striated muscles of the cat was studied by immunohistochemistry. It was found that taurine-like immunoreactivity (TLI) is distributed homogeneously in the cardiac and smooth muscles selected where it is present in every single fibre. In contrast, TLI appeared unevenly distributed in eight out of nine skeletal muscles, the soleus being atypical. Under resting conditions, TLI is present in only a portion of the fibre population, the proportion being specific for each muscle. Under nerve stimulation the TLI negative/positive ratio increased, suggesting release activity, while an opposite trend occurred after denervation. Glutamate-like immunoreactivity (GLI), although present in every fibre, showed a stronger reaction in those fibres positive to taurine, suggesting parallel movements of both amino acids. Results are discussed in terms of two functional possibilities.
Helmet-mounted display (day/night) (1996)

A dangerous situation is created when the pilot looks inside the cockpit for instrument information when flying combat and low altitude missions. While looking at instruments, a pilot cannot be performing situation analysis; yet not looking at instruments runs such risks as flying into the ground, particularly in low visibility conditions or in relatively featureless terrain where visual cues for altitude and attitude are inadequate or deceptive. The AN/AVS-7 HMD solves this problem for night flight for both helicopters and fixed wing aircraft which must operate in a 'nap of the earth' flight regime. The display unit mounts on the AN/AVS-6 night vision goggles and provides symbology overlaid on the pilot's outside view; cockpit instrument information is thus provided through the goggles. The pilot is immediately aware of changes in either his surroundings or the instrument readings. This minimizes the risk of critical information being missed in one area while the pilot is looking in the other. The 'day' HMD version of the AN/AVS-7 display now carries these advantages into daytime flights. This display unit operates in conditions from full sunlight to dusk, provides the same symbology as the night display, and connects to the night display interface with no aircraft modification. The day HMD mounts to the helmet using the attachment points previously reserved for the night vision goggles. This display improves the safety of daytime operations by keeping the eyes 'out of the cockpit' in difficult situations such as those presented during landings, cargo lifting and flight utilizing terrain masking. It offers the possibility of a less stressful way of familiarizing the pilot with the symbology and of the dynamic relationships it has to the aircraft and background motions. This familiarization is now accomplished during night flights using night vision goggles. The 'day' HMD is also a useful maintenance aid, easing the ground crew's checkout of the aircraft systems during the day.
O3‐09‐05: VASCULAR RISK FACTORS AND CSF AD BIOMARKERS ARE INDEPENDENTLY ASSOCIATED WITH COGNITIVE DECLINE (2019)

Although Alzheimer's disease (AD) pathology and cerebrovascular disease commonly co-occur among older adults, it remains unclear whether measures related to vascular disease and AD pathology have independent or synergistic effects on cognitive decline. To address this question, we examined (1) whether midlife vascular risk factors and cerebrospinal fluid (CSF) biomarkers of AD pathology independently or interactively impacted longitudinal cognitive trajectories, and (2) whether vascular risk factors were associated with the accumulation of AD pathology, as measured by change in CSF biomarkers over time. Mixed effects regression models included 168 participants from the BIOCARD Study. At baseline, participants were cognitively normal and primarily middle aged (Table 1). Cognition was measured annually with a composite score consisting of four measures previously shown be the best predictors of risk of progression from normal cognition to Mild Cognitive Impairment in this sample. Baseline vascular risk was assessed with a vascular risk score (VRS) reflecting the presence of five vascular risk factors (diabetes, hypertension, hypercholesterolemia, smoking, obesity). The ratios of CSF total tau and phosphorylated tau to Aβ1-42 (i.e., t-tau/Aβ1-42; p-tau/Aβ1-42) were used as biomarkers of AD pathology at baseline and over time. With longitudinal cognitive trajectories as the dependent variable (M follow-up = 15 years), higher VRS and more abnormal CSF ratios at baseline were each independently associated with a greater rate of cognitive decline (Table 2). However, there were no three-way VRS x CSF ratio x time interactions (both p = .23). With CSF biomarkers as the dependent variable (M follow-up = 2.7 years), the VRS was not associated with level of, or rate of change in, CSF biomarkers (Table 3). Results were similar when CSF Aβ1-42, t-tau, and p-tau were examined individually (rather than ratios).
Bandwidth Enhancement and Further Size Reduction of a Class of Miniaturized Slot Antennas (2004)

In this paper, new methods for further reducing the size and/or increasing the bandwidth (BW) of a class of miniaturized slot antennas are presented. This paper examines techniques such as parasitic coupling and inductive loading to achieve higher BW and further size reduction for this class of miniaturized slot antennas. The overall BW of a proposed double resonant antenna is shown to be increased by more than 94% compared with a single resonant antenna occupying the same area. The behavior of miniaturized slot antennas, loaded with series inductive elements along the radiating section is also examined. The inductive loads are constructed by two balanced short circuited slot lines placed on opposite sides of the radiating slot. These inductive loads can considerably reduce the antenna size at its resonance. Prototypes of a double resonant antenna at 850 MHz and inductively loaded miniaturized antennas at around 1 GHz are designed and tested. Finally the application of both methods in a dual band miniaturized antenna is presented. In all cases measured and simulated results show excellent agreement.
EFFECTS OF LOWERING TEMPERATURE DURING CULTURE ON THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN THE MARINE DIATOM PHAEODACTYLUM TRICORNUTUM (BACILLARIOPHYCEAE)1 (2004)

The composition of fatty acids and contents of eicosapentaenoic acid (EPA) and polyunsaturated fatty acids (PUFAs) of the economically important marine diatom, Phaeodactylum tricornutum (Bohlin), were investigated to see whether reducing the culture temperature enhances the production of EPA and PUFAs. The contents of EPA and PUFAs of P . tricornutum were found to be higher at lower temperature when cultured at 10, 15, 20, or 25°C. When the cells grown at 25°C were shifted to 20, 15, or 10°C, the contents per dry mass of PUFAs and EPA increased to the maximal values in 48, 24, and 12 h, respectively. The highest yields of PUFAs and EPA per unit dry mass (per unit volume of culture) were 4.9% and 2.6% (12.4 and 6.6 mg·L −1 ), respectively, when temperature was shifted from 25 to 10°C for 12 h, both being raised by 120% compared with the control. The representative fatty acids in the total fatty acids, when temperature was lowered from 25 to 10°C, decreased proportionally by about 30% in C 16:0 and 20% in C 16:1(n−7) but increased about 85% in EPA. It was concluded that lowering culture temperature of P . tricornutum could significantly raise the yields of EPA and PUFAs.
Facet Growth of4He Crystals at mK Temperatures (1996)

We have investigated growth of c facets in good quality helium crystals with screw dislocation densities $0--20{\mathrm{cm}}^{\ensuremath{-}2}$ along the c axis. Three distinct regimes of growth were observed. One of them can be explained by spiral growth provided that kinetic energy of moving steps and their tendency to localization at large driving forces are taken into account. In the absence of screw dislocations we find burstlike growth unless the speed is less than 0.5 nm/s, in which case anomalous, intrinsic growth of facets is detected.