gebhart/patrepeval huggingface dataset:
RNA-Binding Protein Rnc1 Regulates Cell Length at Division and Acute Stress Response in Fission Yeast through Negative Feedback Modulation of the Stress-Activated Mitogen-Activated Protein Kinase Pathway (2020)

RNA-binding proteins (RBPs) play a major role during control of mRNA localization, stability, and translation and are central to most cellular processes. In the fission yeast <i>Schizosaccharomyces pombe</i>, the multiple K homology (KH) domain RBP Rnc1 downregulates the activity of the cell integrity pathway (CIP) via stabilization of <i>pmp1</i><sup>+</sup> mRNA, which encodes the Pmp1 phosphatase that inactivates Pmk1, the mitogen-activated protein kinase (MAPK) component of this signaling cascade. However, Rnc1 likely regulates the half-life/stability of additional mRNAs. We show that Rnc1 downregulates the activity of Sty1, the MAPK of the stress-activated MAPK pathway (SAPK), during control of cell length at division and recovery in response to acute stress. Importantly, this control strictly depends on Rnc1's ability to bind mRNAs encoding activators (Wak1 MAPKKK, Wis1 MAPKK) and downregulators (Atf1 transcription factor, Pyp1 and Pyp2 phosphatases) of Sty1 phosphorylation through its KH domains. Moreover, Sty1 is responsible for Rnc1 phosphorylation <i>in vivo</i> at multiple phosphosites during growth and stress, and these modifications trigger Rnc1 for proper binding and destabilization of the above mRNA targets. Phosphorylation by Sty1 prompts Rnc1-dependent mRNA destabilization to negatively control SAPK signaling, thus revealing an additional feedback mechanism that allows precise tuning of MAPK activity during unperturbed cell growth and stress.<b>IMPORTANCE</b> Control of mRNA localization, stability, turnover, and translation by RNA-binding proteins (RBPs) influences essential processes in all eukaryotes, including signaling by mitogen-activated protein kinase (MAPK) pathways. We describe that in the fission yeast <i>Schizosaccharomyces pombe</i> the RBP Rnc1 negatively regulates cell length at division during unperturbed growth and recovery after acute stress by reducing the activity of the MAPK Sty1, which regulates cell growth and differentiation during environmental cues. This mechanism relies on Rnc1 binding to specific mRNAs encoding both enhancers and negative regulators of Sty1 activity. Remarkably, multiple phosphorylation of Rnc1 by Sty1 favors RBP binding and destabilization of the above mRNAs. Thus, posttranscriptional modulation of MAP kinase signaling by RNA-binding proteins emerges as a major regulatory mechanism that dictates the growth cycle and cellular adaptation in response to the changing environment in eukaryotic organisms.
NEW SCLERAL TREPHINE (1913)

Elliot's trephining operation has proved of wonderful service in glaucoma and is now one of the recognized procedures in combating that disease. The technic is simple and the results are equally satisfactory compared with those following iridectomy. Moreover, there is but a minimum of optical disfigurement. The main objections are mechanical and can be attributed to the trephine itself. The hand trephine is difficult to steady and keep in the original incision while applying proper downward pressure and rotary motion. The watch-spring type of corneal trephine cannot be controlled accurately, and the small set-screws, for adjusting the cutting points, are apt to catch the conjunctival flap and badly lacerate it. Therefore I devised the trephine herewith illustrated: A shaft, rotating freely within a rigid housing, is actuated by a flexible shaft at right angles to the main shaft and connected with it by a simple rack-and-pinion bearing. (Fig. 1). The
Perkin 1 Abstracts: Biocatalysis in Organic Synthesis (2000)

Perkin 1 Abstracts: Biocatalysis in Organic Synthesis aims to cover recent literature concerning the applications of enzymes and micro-organisms as catalysts in organic synthesis. The abstracts will emphasise the key synthetic step(s) that are mediated by the biocatalyst. Emerging technologies for biocatalyst design and optimisation will also be included.
Cholecystokinin Receptor Antagonist Halts Progression of Pancreatic Cancer Precursor Lesions and Fibrosis in Mice (2014)

These findings demonstrate that endogenous CCK is in part responsible for the development and progression of pancreatic cancer. The use of CCK receptor antagonists may have a role in cancer prophylaxis in high-risk subjects and may reduce fibrosis in the microenvironment.
Development of a Biocompatible In-Tube Solid-Phase Microextraction Device: A Sensitive Approach for Direct Analysis of Single Drops of Complex Matrixes (2016)

The aim of the current study is to develop a sensitive solid-phase microextraction (SPME) device for direct and rapid analysis of untreated complex matrixes (i.e., single drop of the samples, V ≤ 2 μL). A thin layer of a biocompatible nanostructured polypyrrole (PPy) was electrochemically deposited inside a medical grade spinal needle, minimizing the matrix effect. Microsampling was facilitated by loading the sample inside the in-tube SPME device (withdraw of sample via plunger), where extraction was performed under static conditions. Two strategies were used for analysis of the compounds including offline desorption and running the extract to the liquid chromatograph-tandem mass spectrometer (LC-MS/MS) or direct coupling of the in-tube SPME device to the MS. Given the high surface-area-to-volume ratio of the coating, a short equilibrium time (i.e., t ≤ 2 min) was obtained. The whole analytical procedure (i.e., extraction, rinsing, desorption, and LC-MS/MS analysis) was performed within 10 min by LC-MS/MS, and 3 min by in-tube-MS/MS. Possible matrix effects for the prepared device were evaluated in whole blood samples at three levels of concentration, and encouraging results were achieved in the range of 83-120%. The obtained results, no matrix effect, are attributed to the smooth surface and small pore size of the biocompatible PPy coating, which was prepared in the presence of cetyltrimethylammonium bromide (CTAB) surfactant. The in-tube SPME device was shown to be very sensitive, with high total recoveries obtained for all compounds in phosphate-buffered saline (PBS) and urine samples owing to the large volume and capacity of the coating. Subnanogram per milliliter levels of detection were achieved for urine samples, and low nanogram per milliliter levels were found in whole blood samples for all studied compounds with a high protein binding index. Rapid analysis of whole blood samples was achieved without need of any pretreatment or manipulation of sample, revealing the developed in-tube SPME device as an ideal probe for forensic application, drug monitoring, and point-of care-diagnosis.
Studies on fused indoles. II. Structural modifications and analgesic activity of 4-aminomethyltetrahydrothiopyrano(2,3-b)indoles. (1984)

A series of 4-aminomethyl-2, 3, 4, 9-tetrahydrothiopyrano [2, 3-b] indole derivatives was synthesized and evaluated for analgesic activity. Preliminary structure-activity relationship analysis showed that substitution on the benzene portion of the indole ring reduced the analgesic activity, whereas a short-chain Nb-alkyl substituent enhanced the potency, as exemplified by an Nb-methyl substituted analogue. This compound was equipotent to morphine in the acetic acid writhing assay using mice.
Analysis of 177Lu-DOTA-Octreotate Therapy–Induced DNA Damage in Peripheral Blood Lymphocytes of Patients with Neuroendocrine Tumors (2015)

γ-H2AX can be exploited in the LuTate PRRT as a biomarker of PBL cytotoxicity. Long-term follow-up studies investigating whether elevated residual γ-H2AX values are associated with acute myelotoxicity and secondary blood malignancy may be worthwhile.
Transport Fitness of Cull Sows and Boars: A Comparison of Different Guidelines on Fitness for Transport (2016)

n/a.
Characterization of tomato fruit expressing an antisense gene for pectin methylesterase (1995)

Pectin methylesterase (PME, EC 3.1.1.11) demethoxylates pectins and is believed to be involved in degradation of pectic cell wall components by polygalacturonase in ripening tomato fruit. We have introduced antisense and sense chimeric PME genes into tomato to elucidate the role of PME in fruit development and ripening. Fruits from transgenic plants expressing high levels of antisense PME RNA showed $<$10% of wild-type PME enzyme activity and undetectable levels of PME protein and mRNA. Lower PME enzyme activity in fruits from transgenic plants was associated with an increased molecular weight and methylesterification of pectins and decreased levels of total and chelator soluble polyuronides in cell walls. The fruits of transgenic plants also contained higher levels of soluble solids than did wild-type fruits. Reduction of PME activity in transgenic fruits causes an almost complete loss of tissue integrity during fruit senescence, but shows little effect on fruit firmness during ripening. Low PME activity in the transgenic fruit pericarp selectively impairs accumulation of $\rm Mg\sp{2+}$ over other major cations. Lowered PME activity is associated with a 30 to 70% decrease in bound $\rm Ca\sp{2+}$ and $\rm Mg\sp{2+}$ in transgenic fruit pericarp as compared to wild-type pericarp. Levels of soluble $\rm Ca\sp{2+}$ increase 10 to 60% in transgenic pericarp, while levels of soluble $\rm Mg\sp{2+}$ and $\rm Na\sp+$ are reduced by 20 to 60% with lowered PME activity. Field performance of transgenic tomato plants containing the antisense gene for pectin methylesterase was evaluated during the summers of 1992 and 1993. Field grown transgenic tomato plants had vegetative and fruit yields similar to control lines during both years. Transgenic plants showed an increase in both fruit number and yield in 1992 compared to both wild-type and azygous controls, but no differences were observed among the three genotypes in 1993. Average fruit weight did not show significant differences among the three genotypes in 1992, but was lower in azygous and transgenic plants than in wild-type Rutgers plants in 1993. Transgenic fruits had higher soluble and total solids and higher pH than control fruits, suggesting that transgenic fruits may have better processing quality.
Structured Light: Ideas and Concepts (2020)

The paper briefly presents some essential concepts and features of light fields with strong spatial inhomogeneity of amplitude, phase, polarization, and other parameters. It contains a characterization of optical vortices, speckle fields, polarization singularities. A special attention is paid to the field dynamical characteristics (energy, momentum, angular momentum, and their derivatives), which are considered not only as mechanical attributes of the field but also as its meaningful and application-oriented descriptive parameters. Peculiar features of the light dynamical characteristics in inhomogeneous and dispersive media are discussed. The dynamical properties of paraxial beams and evanescent waves (including surface plasmon–polaritons) are analyzed in more detail; in particular, a general treatment of the extraordinary spin and momentum, orthogonal to the main propagation direction, is outlined. Applications of structured light fields for optical manipulation, metrology, probing, and data processing are described.
Supplementary Figures S1-S14 from Detecting Liver Cancer Using Cell-Free DNA Fragmentomes (2023)

&lt;p&gt;Supplementary Figure S1 shows contributions of individual genomic regions to the final trained DELFI surveillance model. Supplementary Figure S2 shows DELFI scores in individuals without cancer by age and sex. Supplementary Figure S3 shows DELFI scores in high-risk individuals without cancer across racial or ethnic groups. Supplementary Figure S4 shows DELFI scores in cirrhotic patients in relation to severity. Supplementary Figure S5 shows DELFI scores in individuals in relation to BMI. Supplementary Figure S6 Shows Performance of alternative DELFI models. Supplementary Figure S7 shows Correlation between rank ordered DELFI. Supplementary Figure S8 shows DELFI scores in patients with HCC were related to tumor characteristics. Supplementary Figure S9 shows DELFI scores in by etiology of underlying liver disease. Supplementary Figure S10 shows the DELFI score in patients with HCC is correlated to AFP levels. Supplementary Figure S11 shows the correlation of fragmentation profiles. Supplementary Figure S12 shows Genome wide fragmentation profiles in the Hong Kong validation cohort Supplementary Figure S13 shows chromosomal copy number in Hong Kong cohort. Supplementary Figure S14 shows the implementation of DELFI in liver cancer screening.&lt;/p&gt;
EDGE LOADING WEAR IN MODERN ALUMINA CERAMIC-ON-CERAMIC HIP BEARINGS (2005)

Introduction and Aims: The extremely low wear rates of third generation alumina-alumina bearings in traditional hip simulators are not reflected in vivo. Separation of the bearing during swing phase and edge loading with heel strike is reported to account for this discrepancy. Method: We have had the opportunity to visually inspect 21 bearings at re-operation from a group of 1588 hip arthroplasties with third generation alumina ceramic-ceramic bearings. Re-operations were for heterotopic ossification (one), loosening (three), femoral fracture (six), psoas tendonitis (six), sepsis (three) and dislocation (two). There were no re-operations for bearing failure. Sixteen of these 21 bearings (16 heads and 12 inserts) were retrieved and analysed. We mapped the location and we measured the volume of the wear and we performed microscopy and measured roughness of worn and unworn areas. Results: Eleven bearings had visual evidence of edge loading wear, making an incidence of 52% in the 21 patients having re-operations. These 11 bearings and five visually undamaged bearings were analysed. The wear on the insert was always located at the rim indicating edge loading. The location and orientation of the stripe on the head was not consistent with subluxation during normal gait but was consistent with subluxation and edge loading with the hip flexed at 90 degrees. The average wear volume was 0.7mm3 per year (heads plus liners). Longer service bearings had signs under SEM of repolishing of the wear area suggesting that the process of edge loading wear will be self-limiting. The heads without a wear scar showed very little damage: under SEM, a slight relief polishing of individual grains and minor pitting was noted. Conclusion: The subluxation causing the stripe wear in these patients did not occur during normal walking gait. It probably occurred with rising from a chair. Simulator testing of third generation alumina-alumina components must include edge loading if it is to give a realistic indication of in vivo performance. Alumina-alumina bearings remain an excellent option for total hip arthroplasty, however more work is required to understand the clinical consequences.
Large Metal Objects As Near Field UHF RFID Antennas (2017)

This paper demonstrates that a long thin metal structure can act as a near-field antenna for a passive UHF RFID system. It is demonstrated that a conventional metal mountable tag can be detected up to 30 m along a metal bar from the feed point far exceeding the maximum far field free space range of the tag when mounted on metal. Further system improvements are achieved by altering the physical length of the metal structure or by changing the carrier frequency used. It is demonstrated that the fields generated along the structure (e.g., E-field or H-field) dictate the antenna type which will be best suited for coupling to it. A conventional passive tag utilizing a dipole antenna and oriented normal to the metal surface efficiently harvests the E-field. It is deduced that the tag can be continuously detected up to at least 50 m along a bar from the antenna feed.
Highly selective and sensitive chemosensor based on 2,3-diaminophenazine hydrochloride for the detection of cyanide in pure water and its application in plant seed samples (2018)

A 2,3-diaminophenazine hydrochloride (<bold>Q1</bold>) shows a ultrasensitive visible and fluorescence response for cyanide in pure water <italic>via</italic> hydrogen bonding.
Retracted Article: Laser produced plasma diagnosis of carcinogenic heavy metals in gallstones (2015)

Gall bladder cancer [GBC] is a highly fatal malignancy.
Evaluation of protein‐denaturing immunoassays for avidity of immunoglobulin G to rubella virus (1994)

Immunoassays have been recently developed that measure the avidity of IgG antibodies to complex microbial antigens and are suitable for serologic diagnosis of infectious diseases. In these avidity ELISAs, protein-denaturing agents are applied either as diluents of patient sera to prevent the immune complexing of IgG (diluting principle), or the preformed complexes are treated with the protein denaturants (eluting principle). We compared four protein denaturants previously used in such assays, in a diagnostic avidity ELISA for rubella IgG. Diethylamine, guanidine, thiocyanate, or urea were applied, by either principle at various concentrations, and thiocyanate, or urea were applied, by either principle at various concentrations, and thiocyanate at an optimum pH. Patient sera obtained recently after primary infection were distinguished from sera representing past rubella immunity by any protein denaturant tested by the eluting principle, which was superior to the diluting principle.
Component Optimization for Catalyst Layers in Proton Exchange Membrane Fuel Cells (2020)

Proton exchange membrane fuel cells (PEMFCs) have been recognized as a promising energy conversion solution. The biggest challenges for the commercialization of PEMFCs are cost and durability. Although many efforts have been made on catalysts, the performance and durability of the membrane electrode assemblies (MEAs) still cannot fully meet the targets established by the U.S. Department of Energy. Optimizing the catalyst layer to maximize the utilization of catalysts is a quite practical issue. In this paper, we reviewed the most recent advances on the component optimization for catalyst layers, including support materials, ionomers, and solvents. Based on the reaction mechanism on the three-phase boundary, enhancing the intrinsic properties of each component and optimizing their interactions can attribute a lot to the performance and durability of PEMFCs.
Clioquinol Decreases Amyloid-β Burden and Reduces Working Memory Impairment in a Transgenic Mouse Model of Alzheimer's Disease (2009)

Clioquinol (CQ) is a "metal protein attenuating compound" that crosses the blood-brain barrier and binds, with high affinity, copper(II) and zinc(II), two metal ions critically involved in amyloid-beta aggregation and toxicity. CQ was recently proposed for the treatment of Alzheimer's disease, but controversial data have been reported so far concerning its real therapeutic advantages. We describe here results of chronic CQ treatment in the TgCRND8 mouse model of Alzheimer's disease. Remarkably, based on classical behavioral tests, CQ treatment was found to reverse, to a large extent, the working memory impairments that are characteristic of this mouse model. Pairwise, a significant reduction of amyloid-beta plaque burden, both in the cortex and in the hippocampus, was detected as well as an attenuation of astrogliosis. MALDI Mass Spectrometry Imaging technique revealed a specific localization of CQ in the above mentioned brain areas. Modest but significant effects on the absolute and relative brain concentrations of the three most important biometals (i.e., copper, zinc, and iron) were highlighted following CQ treatment. The pharmacological and mechanistic implications of the above findings are thoroughly discussed.
Robust High Performance Bilateral Teleoperation Under Bounded Time-Varying Dynamics (2014)

The control design for bilateral teleoperation systems represents a challenge in finding the proper balance in the inherent tradeoff between transparency and stability. To address this problem, we propose a methodology in which we develop a parametric model of the teleoperation system. Subsequently, we exploit robust control techniques based on linear matrix inequalities to design controllers that aim to achieve a predefined performance, and are robust to bounded but arbitrarily fast-time-varying parametric uncertainties. We present analysis, simulation, and experimental results of the designed controller, thus showing that the assumptions made during modeling are appropriate and the effectiveness of the method to tradeoff perfect transparency and stability.
Non-digestible oligosaccharides partially prevent the development of LPS-induced lung emphysema in mice (2019)

Chronic obstructive pulmonary disease (COPD), a progressive inflammatory disorder, is also known as a systemic inflammatory disease, in which the gut-lung interaction plays an important role. The use of non-digestible oligosaccharides (NDOs) has gained attention in airway diseases due to their systemic effects on inflammatory markers. Here, the preventive effects of specific non-digestible oligosaccharides (GOS/lcFOS/lvPectin) were investigated in a murine lipopolysaccharide (LPS)-induced emphysema model. Nasal LPS-installations were used to induce emphysema in male BALB/c mice. Two weeks prior to the first LPS challenge, mice received GOS/lcFOS/lvPectin (9:1:2) mixture by gavage (25 mg NDOs/200 μl PBS) five days a week until day 28. The LPS-induced neutrophil influx in bronchoalveolar lavage fluid (BALF) decreased by >60% after intervention with GOS/lcFOS/lvPectin and the development of lung emphysema, measured by mean linear intercept, was prevented. Macroscopic examination of heart tissue revealed that GOS/lcFOS/lvPectin pretreatment attenuated the LPS-induced increase in right ventricular heart hypertrophy. In summary, GOS/lcFOS/lvPectin prevented characteristic features of COPD in the LPS-induced lung emphysema model. Since no therapy is available to stop or prevent development of COPD, oligosaccharides may have potential to be used as stand alone or in combination with other anti-inflammatory nutrients or drugs to diminish disease progression in COPD.
The interplay of iron(ii) spin transition and polymorphism (2012)

The mononuclear compound (1) [Fe(II)(L)(2)](BF(4))(2) (L = 4-ethynyl-2,6-bis(pyrazol-1-yl)pyridine) was prepared and structurally as well as magnetically characterised. The crystallisation revealed the formation of two polymorphs--the orthorhombic 1A and the tetragonal form 1B. A third, intermediate phase 1C was found exhibiting a different orthorhombic space group. Reversibility of the phase transition between 1A and 1C was studied by variable-temperature single-crystal and powder X-ray diffraction studies, while an irreversible phase transition was observed for the transition of 1B→1C. The magnetic studies show that the 1A↔1C transition is accompanied by a very abrupt spin transition (ST) with 8 K hysteresis width (T(1/2)(↓) = 337 K, T(1/2)(↑) = 345 K). The ST was confirmed by Mössbauer spectroscopy as well as by DSC studies. In contrast, the 1B polymorph remained low-spin up to 420 K. In conclusion, a full cycle of intertwined phase- and spin-conversions of three polymorphs could be proven following the general scheme 1B→1C↔1A.
Isoimmunization to the Rh Antigen E in a Person with Genes CDe (1948)

Journal Article Isoimmunization to the Rh Antigen E in a Person with Genes CDe Get access W. G. Rice, M.D., W. G. Rice, M.D. From the Canadian Red Cross Blood Transfusion Service, Vancouver, British Columbia, Canada Search for other works by this author on: Oxford Academic Google Scholar Faith G. Watson Faith G. Watson From the Canadian Red Cross Blood Transfusion Service, Vancouver, British Columbia, Canada Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 18, Issue 8, 1 August 1948, Pages 598–601, https://doi.org/10.1093/ajcp/18.8.598 Published: 01 August 1948 Article history Received: 19 April 1948 Published: 01 August 1948