gebhart/patrepeval huggingface dataset:
Delineation of two multi-invasion-induced rearrangement pathways that differently affect genome stability (2023)

Punctuated bursts of structural genomic variations (SVs) have been described in various organisms, but their etiology remains incompletely understood. Homologous recombination (HR) is a template-guided mechanism of repair of DNA double-strand breaks and stalled or collapsed replication forks. We recently identified a DNA break amplification and genome rearrangement pathway originating from the endonucleolytic processing of a multi-invasion (MI) DNA joint molecule formed during HR. Genome-wide approaches confirmed that multi-invasion-induced rearrangement (MIR) frequently leads to several repeat-mediated SVs and aneuploidies. Using molecular and genetic analysis and a novel, highly sensitive proximity ligation-based assay for chromosomal rearrangement quantification, we further delineate two MIR subpathways. MIR1 is a universal pathway occurring in any sequence context, which generates secondary breaks and frequently leads to additional SVs. MIR2 occurs only if recombining donors exhibit substantial homology and results in sequence insertion without additional breaks or SVs. The most detrimental MIR1 pathway occurs late on a subset of persisting DNA joint molecules in a PCNA/Pol<i>δ</i>-independent manner, unlike recombinational DNA synthesis. This work provides a refined mechanistic understanding of these HR-based SV formation pathways and shows that complex repeat-mediated SVs can occur without displacement DNA synthesis. Sequence signatures for inferring MIR1 from long-read data are proposed.
Poly[N-(2-hydroxypropyl)methacrylamide]-Based Tissue-Embedding Medium Compatible with MALDI Mass Spectrometry Imaging Experiments (2011)

Traditional tissue-sectioning techniques for histological samples utilize various embedding media to stabilize the tissue on a sectioning target and to provide a smooth cutting surface. Due to the ion suppression effect in MALDI ionization and number of background peaks in the low-mass region, these media are not suitable for mass spectrometry imaging (MSI) experiments. To overcome this, droplets of water are often used to mount the tissue on a sectioning target, but the ice block formed around the tissue does not provide a good support for sectioning of fragile samples. In this work, we propose a novel embedding media, compatible with MALDI ionization and MSI experiments, based on poly[N-(2-hydroxypropyl)methacrylamide] (pHPMA). Using a reversible addition-fragmentation chain transfer polymerization technique, well-defined pHPMA polymer with narrow mass distribution was prepared. Benefits of the resulted pHPMA-based embedding media were tested on different tissue samples.
TWO SATURABLE ABSORBERS FOR EXTENDING THE WAVELENGTH LIMITS OF A PASSIVELY Q-SWITCHED CO2 LASER (1969)

The discovery of two new passive Q-switching agents for use in the CO2 laser is reported. These gases, chlorotrifluoroethylene and difluorodichloromethane, permit extension of the range of wavelengths over which the passively Q-switched CO2 laser can be operated. Saturation intensities and small signal absorption coefficients were measured for these gases.
New cephalosporins and 7.ALPHA.-methoxy cephalosporins chemistry and biological activities. (1979)

The synthesis and the in vitro activity of a number of cephalosporins and 7 alpha-methoxy cephalosporins having 7-acyl substituents derived from 1-methyl-4 (or 5)-nitro-1H-imidazolyl-thioacetic acids are described. The microbiological profile is influenced by the position of both the nitro group and the side-chain sulfur atom on the 1-methyl imidazole, and by the nature of the 3-substituent.
Mesenchymal stem cells preserve neonatal right ventricular function in a porcine model of pressure overload (2016)

Limited therapies exist for patients with congenital heart disease (CHD) who develop right ventricular (RV) dysfunction. Bone marrow-derived mesenchymal stem cells (MSCs) have not been evaluated in a preclinical model of pressure overload, which simulates the pathophysiology relevant to many forms of CHD. A neonatal swine model of RV pressure overload was utilized to test the hypothesis that MSCs preserve RV function and attenuate ventricular remodeling. Immunosuppressed Yorkshire swine underwent pulmonary artery banding to induce RV dysfunction. After 30 min, human MSCs (1 million cells, n = 5) or placebo (n = 5) were injected intramyocardially into the RV free wall. Serial transthoracic echocardiography monitored RV functional indices including 2D myocardial strain analysis. Four weeks postinjection, the MSC-treated myocardium had a smaller increase in RV end-diastolic area, end-systolic area, and tricuspid vena contracta width (P < 0.01), increased RV fractional area of change, and improved myocardial strain mechanics relative to placebo (P < 0.01). The MSC-treated myocardium demonstrated enhanced neovessel formation (P < 0.0001), superior recruitment of endogenous c-kit+ cardiac stem cells to the RV (P < 0.0001) and increased proliferation of cardiomyocytes (P = 0.0009) and endothelial cells (P < 0.0001). Hypertrophic changes in the RV were more pronounced in the placebo group, as evidenced by greater wall thickness by echocardiography (P = 0.008), increased cardiomyocyte cross-sectional area (P = 0.001), and increased expression of hypertrophy-related genes, including brain natriuretic peptide, β-myosin heavy chain and myosin light chain. Additionally, MSC-treated myocardium demonstrated increased expression of the antihypertrophy secreted factor, growth differentiation factor 15 (GDF15), and its downstream effector, SMAD 2/3, in cultured neonatal rat cardiomyocytes and in the porcine RV myocardium. This is the first report of the use of MSCs as a therapeutic strategy to preserve RV function and attenuate remodeling in the setting of pressure overload. Mechanistically, transplanted MSCs possibly stimulated GDF15 and its downstream SMAD proteins to antagonize the hypertrophy response of pressure overload. These encouraging results have implications in congenital cardiac pressure overload lesions.
Alterations in erythrocyte membrane transporter expression levels in type 2 diabetic patients (2021)

Type 2 diabetes mellitus (T2DM) is one of the most common multifactorial diseases and several membrane transporters are involved in its development, complications and treatment. We have recently developed a flow-cytometry assay panel for the quantitative determination of red cell membrane protein levels with potential relevance in diseases. Here we report a detailed phenotypic analysis of a medium scale, clinically based study on the expression of T2DM-related membrane proteins, the GLUT1, GLUT3, MCT1, URAT1, ABCA1, ABCG2 and the PMCA4 transporters in erythrocytes. By comparing age-matched control subjects and three groups of T2DM patients (recently diagnosed, successfully managed, and patients with disease-related complications), we found significant differences in the membrane expression levels of the transporters in these groups. This is a first detailed analysis of T2DM related alterations in erythrocyte membrane transporter protein levels, and the results suggest significant changes in some of the transporter expression levels in various patient groups. By performing a further, more detailed analysis of the clinical and molecular biology parameters, these data may serve as a basis of establishing new, personalized diagnostic markers helping the prevention and treatment of type 2 diabetes.
Evaluation of anti–apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide (2011)

This study reveals and proves the anticancer properties. However, in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging, and significantly protect nucleus and bcl-2.
New strongly coupled dinuclear metal centres in organometallic s-indacene complexes (1997)

The complexes cis-[{Fe(CO) 3 } 2 L] and cis-[{Co(CO) 2 } 2 L] are prepared from 1,3,5,7-tetra-tert-butyl-s-indacene; in solution, the diiron complexes exhibit very strong electron–electron coupling between iron centres as evidenced by a 810 mV separation between the 0/+ and +/2+ redox waves.
Aripiprazole for Irritability Associated with Autistic Disorder in Children and Adolescents Aged 6–17 Years (2010)

Aripiprazole was recently US FDA-approved to treat irritability in children and adolescents with autistic disorder aged 6-17 years. There are currently only two psychotropics approved by the FDA to treat irritability in the autistic population. This drug profile will discuss available studies of aripiprazole in individuals with pervasive developmental disorders, two of which led to its recent FDA approval. We will discuss the efficacy, as well as the safety and tolerability of the drug documented in these studies. In addition, the chemistry, pharmacokinetics, metabolism and mechanism of action of aripiprazole will be reviewed.
Synthesis and Mechanical Properties of Fully Dense Ti 2 SC (2007)

Because the layered machinable ternary carbide, Ti 2 SC, has a significantly shorter c ‐lattice parameter—as compared with most of the 50+ other so‐called M n+1 AX n (MAX) phase family (M=early transition metal; A=A group element and X=C or N and n=1–3) to which it belongs—it was postulated that its mechanical properties would be significantly different than the other MAX phases. In this work, fine‐grained (FG) and coarse‐grained (CG) polycrystalline fully dense Ti 2 SC samples were fabricated. Hot pressing Ti 2 SC powders, at 1500°C under a stress of ∼45 MPa for 5 h resulted in FG (2–4 μm) samples which and upon further annealing for 20 h at 1600°C resulted in CG (10–20 μm) ones. No peaks other than those associated with Ti 2 SC and an impurity anatase phase, with a volume fraction of ∼6 vol% were observed in the XRD patterns and micrographs. The average Vickers hardness in the 2–300 N range is 8±2 GPa with the FG samples being slightly harder. This hardness is the highest of any of the MAX phases characterized to date. Also in contrast to all MAX phases, cracks extended from the corners of Vickers indents in the FG samples. From these cracks the fracture toughness was estimated and found to increase more or less linearly with load from ≈4 to 6 MPa·m 1/2 as the Vickers force was increased from 50 to 300 N, respectively. The room temperature compressive stress of the FG samples was 1.4±0.2 GPa; the failure mode was brittle. Its Young's modulus—also one of the highest for a M 2 AX phase measured to date—was 316±2 GPa. There was no evidence for incipient kink band formation during simple compression. The latter is attributed, in part, to the fine grain size of the hot‐pressed material.
Nuclear accumulation of multiple protein kinases during prolactin-induced proliferation of Nb2 rat lymphoma cells (1996)

Intracellular kinases play important roles in signal transduction and are involved in the surface receptor-mediated regulation of cellular functions, including mitogenesis. In the present study, we examined the possible involvement of various protein kinases in the passage of a mitogenic signal from the cell surface to the nucleus of Nb2 cells, a rat nodal lymphoma cell line in which prolactin is a mitogen. Following a prolactin challenge, various kinase activities were monitored at short intervals in different cellular fractions over a 60 min period. Protein kinase C (PKC) activity in the cytosolic fraction rapidly declined to 50% of its original activity within the first 30 min, while PKC activity in the nuclear fractions increased sharply, reaching its highest level by 30 min following a prolactin challenge. There were also increases in both casein kinase and protein tyrosine kinase (PTK) activities in the nuclear fractions during the first 30 min following a prolactin challenge that paralleled PKC activity. The activities of all three kinases declined thereafter, reaching levels close to their respective basal values by 60 min following initiation of prolactin treatment. These observations suggest the possibility that multiple protein kinases may be involved in mitogenic signal transduction for prolactin in Nb2 cells.
Synthetic and structural studies on monomeric olefin and isocyanide complexes of copper(I): (diethylenetriamine)(1-hexene)copper(I) tetraphenylborate and (N,N,N',N'-tetramethylethylenediamine)bis(cyclohexyl isocyanide)copper(I) tetraphenylborate (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic and structural studies on monomeric olefin and isocyanide complexes of copper(I): (diethylenetriamine)(1-hexene)copper(I) tetraphenylborate and (N,N,N',N'-tetramethylethylenediamine)bis(cyclohexyl isocyanide)copper(I) tetraphenylborateMarco Pasquali, Carlo Floriani, A. Gaetani-Manfredotti, and A. Chiesi-VillaCite this: Inorg. Chem. 1979, 18, 12, 3535–3542Publication Date (Print):December 1, 1979Publication History Published online1 May 2002Published inissue 1 December 1979https://pubs.acs.org/doi/10.1021/ic50202a048https://doi.org/10.1021/ic50202a048research-articleACS PublicationsRequest reuse permissionsArticle Views106Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Broadband Cross-Slotted Patch Antenna for 5G Millimeter-Wave Applications Based on Characteristic Mode Analysis (2022)

This article proposes a wideband differentially fed dual-polarized magnetoelectric (ME) dipole for millimeter-wave (mm-Wave) applications. Various electric and magnetic characteristic modes of a slotted patch antenna are investigated and utilized effectively to create a stable broadside radiation pattern, covering fifth-generation (5G) frequency bands from 24.25 to 40 GHz. To implement this, the lifted ground (LGND) concept is applied to achieve a 57.1% impedance bandwidth for a single antenna element. In addition, the three resonances of the antenna can be manipulated independently. The use of differential feeding allows more than 36 dB of port-to-port isolation across the entire operating band. The measured gains of the single element and <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$2\times $ </tex-math></inline-formula> 2 array are 8.4 and 13.4 dBi, respectively. Also, the measured results indicate symmetrical E- and H-plane radiation patterns and cross-polarization levels lower than −26 dB. With the favorable electrical performance, compact size, simple structure, and low-cost fabrication, the proposed ME dipole is a promising candidate for mm-Wave Antenna-in-Package (AiP) applications.
O-Linked-N-Acetylglucosaminylation of the RNA-Binding Protein EWS N-Terminal Low Complexity Region Reduces Phase Separation and Enhances Condensate Dynamics (2021)

Many membraneless organelles are thought to be biomolecular condensates formed by phase separation of proteins and other biopolymers. Post-translational modifications (PTMs) can impact protein phase separation behavior, although for many PTMs this aspect of their function is unknown. <i>O</i>-linked β-D-<i>N</i>-acetylglucosaminylation (<i>O</i>-GlcNAcylation) is an abundant form of intracellular glycosylation whose roles in regulating biomolecular condensate assembly and dynamics have not been delineated. Using an <i>in vitro</i> approach, we found that <i>O</i>-GlcNAcylation reduces the phase separation propensity of the EWS <i>N</i>-terminal low complexity region (LCR<sub>N</sub>) under different conditions, including in the presence of the arginine- and glycine-rich RNA-binding domains (RBD). <i>O</i>-GlcNAcylation enhances fluorescence recovery after photobleaching (FRAP) within EWS LCR<sub>N</sub> condensates and causes the droplets to exhibit more liquid-like relaxation following fusion. Following extended incubation times, EWS LCR<sub>N</sub>+RBD condensates exhibit diminished FRAP, indicating a loss of fluidity, while condensates containing the <i>O</i>-GlcNAcylated LCR<sub>N</sub> do not. In HeLa cells, EWS is less <i>O</i>-GlcNAcylated following <i>OGT</i> knockdown, which correlates with its increased accumulation in a filter retardation assay. Relative to the human proteome, <i>O</i>-GlcNAcylated proteins are enriched with regions that are predicted to phase separate, suggesting a general role of <i>O</i>-GlcNAcylation in regulation of biomolecular condensates.
Low rank and sparse matrix reconstruction with partial support knowledge for surveillance video processing (2013)

It has been shown recently that incorporating priori knowledge into the basic compressive sensing results in significant improvement of its performance. This has motivated us to extend the incorporation of partial known support into the problem of Robust Principal Component Analysis (RPCA) from compressive measurements. Our proposed algorithm utilizes the known part of the support to recover a matrix as the sum of a low-rank matrix and a sparse component and is tested on the problem of surveillance video reconstruction from compressive measurements. Our experimental results show that the incorporation of partial known support, can significantly improve the reconstruction performance of video sequences.
Merocyanine dyes: synthesis, structure, properties and applications (2009)

Key approaches to the synthesis of merocyanine dyes are summarised. The dependence of the dye properties on their chemical structure, the solvent nature and the aggregation type is analysed. The main methods of studying the electronic structure of merocyanines are considered. Attention is focused on polyene–polymethine electron transitions. The applications of merocyanines in modern research, including the design of new materials and technologies (non-linear optics, photovoltaics, holography, etc.) are presented.
Design of a randomized controlled phase III study of dose dense methotrexate, vinblastine, doxorubicin and cisplatin (dd-MVAC) or gemcitabine and cisplatin (GC) as peri-operative chemotherapy for patients with locally advanced transitional cell cancer of the bladder. The French GETUG/AFU V05 VESPER trial (2020)

The main objective of the French GETUG/AFU V05 VESPER randomized phase III study was to assess the efficacy of dd-MVAC and GC in term of progression-free survival in patients for whom chemotherapy has been decided, before or after surgery. A total of 500 patients have been randomized in 28 reference centers. Inclusion criteria were urothelial carcinoma without neuro-endocrine variant, disease defined by a T2, T3 or T4a N0 (pelvic lymph node ≤ 10 mm on CT scan) M0 staging for patients receiving neoadjuvant chemotherapy or pT3 or pT4 or pN+ and M0 for patients receiving adjuvant chemotherapy. Secondary endpoints include overall survival, safety, response rate. The peri-operative chemotherapy schedule was experimental arm dd-MVAC for a total of 6 cycles versus standard arm GC 4 cycles. The toxicity was evaluated according to NCI CTCAE (v 4.0). The progression-free survival rate will be estimated at 3 years by the Kaplan-Meier method. All the patients will be followed for 5 years. The last patient was randomized in March 2018 and the primary endpoint results are expected for mid-2021. As the dd-MVAC schedule is associated with higher response rates in metastatic disease, the real question today is to confirm such benefit in the peri-operative setting, taking also in consideration the chemotherapy toxicity. Tomorrow, the challenge may be the best chemotherapy and immunotherapy association, the authors hope that final Vesper Trial results will help to determine the gold standard chemotherapy.
13C-breath tests and liver fibrosis. (2004)

The evaluation of the presence and degree of liver fibrosis in patients with chronic liver disease is a fundamental diagnostic and prognostic issue. This is mainly due to the repercussions of liver fibrosis on liver function, whose derangement, in turn, is mainly responsible for the negative events of advanced liver disease. 13C-Breath Tests ((13/14)C-BTs) for the study of liver function were developed more than twenty years ago in order to non-invasively assess residual liver function in patients with various degrees of liver fibrosis, from minimal stages up to liver cirrhosis. Sequential studies that were performed over the years using various 13C-BT substrates showed that increasing degrees of liver fibrosis are paralleled by concomitant modifications in 13C-BT results. The 13C-BT probes that reportedly obtained interesting results were aminopyrine, galactose, and more recently phenylalanine. As the knowledge in this field evolved, probes for the study of specific functions, such as the 13C-Octanoate Breath Test were sought. Analysis of the published studies would seem to show that 13C-BTs alone, or in combination may provide a non-invasive picture of the functional alterations secondary to liver fibrosis. Further studies are needed to evaluate the diagnostic yield of the 13C-BT in particular clinical situations, such as in patients with normal static parameters of liver function, or after therapy.
[Evaluation of a formula for optimal positioning of a central venous catheter through the right internal jugular vein]. (2003)

The aforementioned formula predicted appropriate placement of the catheter tip in 94.54% of the patients.
Island Nucleation in a Reactive Two-Component System (2001)

The flux and temperature dependence of titanium silicide islands formed by reactive deposition near 500 degrees C indicate a critical nucleus containing 2 Ti atoms and a single activation energy of E(d) + 1/2E(2) = 1.4 +/- 0.2 eV, where E(d) and E(2) are the surface diffusion and cluster binding energies, respectively. These values are not consistent with STM observations of Ti dimer-vacancy hopping at lower temperatures and show that silicide island nucleation involves a different, highly mobile Ti species.
KL-Nucleus Total Cross Sections between 30 and 150 GeV: Quantitative Evidence for Inelastic Screening (1979)

We have obtained high-statistics, virtually systematics-free ${K}_{L}$-nucleus total-cross-section data for C, Al, Cu, Sn, and Pb from 30 to 150 GeV. These cross sections have an ${A}^{0.84\ifmmode\pm\else\textpm\fi{}0.01}$ dependence over most of the momentum range, in agreement with the Glauber-Franco model. Their magnitudes are, however, not compatible with that model alone: The discrepancies are clearly resolved once one allows for inelastic screening within the nucleus. The data strongly support the theory of this latter effect as given by Karmanov and Kondratyuk.