gebhart/patrepeval huggingface dataset:
Data from Single-Cell Profiling Reveals Metabolic Reprogramming as a Resistance Mechanism in <i>BRAF</i>-Mutated Multiple Myeloma (2023)

&lt;div&gt;AbstractPurpose:&lt;p&gt;Although remarkably effective in some patients, precision medicine typically induces only transient responses despite initial absence of resistance-conferring mutations. Using &lt;i&gt;BRAF&lt;/i&gt;-mutated myeloma as a model for resistance to precision medicine we investigated if &lt;i&gt;BRAF-&lt;/i&gt;mutated cancer cells have the ability to ensure their survival by rapidly adapting to BRAF inhibitor treatment.&lt;/p&gt;Experimental Design:&lt;p&gt;Full-length single-cell RNA (scRNA) sequencing (scRNA-seq) was conducted on 3 patients with &lt;i&gt;BRAF&lt;/i&gt;-mutated myeloma and 1 healthy donor. We sequenced 1,495 cells before, after 1 week, and at clinical relapse to BRAF/MEK inhibitor treatment. We developed an &lt;i&gt;in vitro&lt;/i&gt; model of dabrafenib resistance using genetically homogeneous single-cell clones from two cell lines with established &lt;i&gt;BRAF&lt;/i&gt; mutations (U266, DP6). Transcriptional and epigenetic adaptation in resistant cells were defined by RNA-seq and H3K27ac chromatin immunoprecipitation sequencing (ChIP-seq). Mitochondrial metabolism was characterized by metabolic flux analysis.&lt;/p&gt;Results:&lt;p&gt;Profiling by scRNA-seq revealed rapid cellular state changes in response to BRAF/MEK inhibition in patients with myeloma and cell lines. Transcriptional adaptation preceded detectable outgrowth of genetically discernible drug-resistant clones and was associated with widespread enhancer remodeling. As a dominant vulnerability, dependency on oxidative phosphorylation (OxPhos) was induced. In treated individuals, OxPhos was activated at the time of relapse and showed inverse correlation to MAPK activation. Metabolic flux analysis confirmed OxPhos as a preferential energetic resource of drug-persistent myeloma cells.&lt;/p&gt;Conclusions:&lt;p&gt;This study demonstrates that cancer cells have the ability to rapidly adapt to precision treatments through transcriptional state changes, epigenetic adaptation, and metabolic rewiring, thus facilitating the development of refractory disease while simultaneously exposing novel vulnerabilities.&lt;/p&gt;&lt;/div&gt;
Calculation and comparison of the surface ionization ϕ(0) (1990)

Abstract An analytical expression for the surface ionization ϕ(O) is derived from fundamentals, viz., the ionization cross section and the angular and energy distribution of backscattered electrons. In order to avoid numerical integrations, a simple formula for the energy distribution of backscattered electrons is presented. The values given by the resulting expression for ϕ(O) are compared with those given by already known formulae and with experimental findings. In general, the agreement is quite satisfactory, although the calculated values generally are smaller than those experimentally found, especially at high overvoltages. This can at least partly be explained by secondary fluorescence excited by the continuous radiation, of which the correction has not been taken into account in the experimental determination of the ϕ(O) values.
Image completion algorithm based on texture synthesis (2007)

A new algorithm is proposed for completing the missing parts caused by the removal of foreground or background elements from an image of natural scenery in a visually plausible way. The major contributions of the proposed algorithm are: (1) for most natural images, there is a strong orientation of texture or color distribution. So a method is introduced to compute the main direction of the texture and complete the image by limiting the search to one direction to carry out image completion quite fast; (2) there exists a synthesis ordering for image completion. The searching order of the patches is defined to ensure the regions with more known information and the structures should be completed before filling in other regions; (3) to improve the visual effect of texture synthesis, an adaptive scheme is presented to determine the size of the template window for capturing the features of various scales. A number of examples are given to demonstrate the effectiveness of the proposed algorithm.
A general method for the synthesis of isatins (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA general method for the synthesis of isatinsPaul G. Gassman, Berkeley W. Cue Jr., and Tien-Yau LuhCite this: J. Org. Chem. 1977, 42, 8, 1344–1348Publication Date (Print):April 1, 1977Publication History Published online1 May 2002Published inissue 1 April 1977https://pubs.acs.org/doi/10.1021/jo00428a016https://doi.org/10.1021/jo00428a016research-articleACS PublicationsRequest reuse permissionsArticle Views1973Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Nanoliter volume sequential differential concentration gradient detector (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNanoliter volume sequential differential concentration gradient detectorJanusz. PawliszynCite this: Anal. Chem. 1988, 60, 24, 2796–2801Publication Date (Print):December 15, 1988Publication History Published online1 May 2002Published inissue 15 December 1988https://pubs.acs.org/doi/10.1021/ac00175a031https://doi.org/10.1021/ac00175a031research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Time-Resolved Diffraction Studies of Nanoscale Thermal Transport (2007)

The synthesis of a series of allene complexes (POCOP)Ir(η²-RC=(.)=CR') 1b-4b (POCOP = 2,6-bis(di-tert-butylphosphonito)benzene) via isomerization of internal alkynes is reported. We have demonstrated that the application of this methodology is viable for the isomerization of a wide variety of alkyne substrates. Deuterium labeling experiments support our proposed mechanism. The structures of the allene complexes 1b-4b were determined using spectroscopic data analysis. Additionally, the solid-state molecular structure of complex 2b was determined using single crystal X-ray diffraction studies and it confirmed the assignment of an iridium-bound allene isomerization product. The rates of isomerization were measured using NMR techniques over a range of temperatures to allow determination of thermodynamic parameters. Finally, we report a preliminary step towards developing a catalytic methodology; the allene may be liberated from the metal center by exposure of the complex to an atmosphere of carbon monoxide.
ChemInform Abstract: A Brand‐New Pd‐Mediated Generation of Benzyne and Its [2 + 2 + 2] Cycloaddition: δ‐Carbon Elimination and Concomitant Decarboxylation. (2009)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Novel (Bio)chemical and (Photo)physical Probes for Imaging Living Cells (2005)

The living cell mediates its internal state and the exchange of substances and information with its environment primarily via protein-protein interactions. The spatio-temporal disposition of structural, catalytic, and regulatory proteins defines the nature and functional state of the cell. Signaling mechanisms, as a prominent example, occupy a central role in this process, leading to a set of canonical questions, challenges and strategies (Table 1).
Substrate-related potent inhibitors of brain metalloendopeptidase (1988)

Rat brain metalloendopeptidase (EC 3.4.24.15) generates Leu- and Met-enkephalin from several larger opioid peptides and is capable of degrading a number of neuropeptides. Substrate-related N-(1-carboxy-3-phenylpropyl) peptide derivatives were synthesized and tested for enzyme inhibition. The best of these derivatives, N-[1(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate, inhibited the enzyme in a competitive manner with a Ki of 16 nM. The data indicate that the carboxyl group of the N-(1-carboxy-3-phenylpropyl) moiety coordinates with the active site zinc atom and that the remaining part of the inhibitor is necessary for interaction with the substrate recognition site of the enzyme. Replacement of the 1-carboxy-3-phenylpropyl group by a carboxymethyl group decreased the inhibitory potency by more than 3 orders of magnitude, emphasizing the importance of the hydrophobic phenyl group for inhibitor binding to a hydrophobic pocket at the S1 subsite. Replacement of the Tyr residue by an Ala residue decreased the inhibitory potency by more than 20-fold. Changes in the structure of the residue interacting with the S1' subsite could cause a more than 60-fold change in inhibition. The inhibitors were either ineffective or only weakly inhibitory against membrane-bound metalloendopeptidase ("enkephalinase", EC 3.4.24.11), an enzyme highly active in rabbit kidney but also present in brain. The data indicate the presence of an extended binding site in the enzyme with residues interacting with S1, S1', and S3' subsites largely determining inhibitor binding.(ABSTRACT TRUNCATED AT 250 WORDS)
The Heart of Microsurgery (1998)

This article highlights an important milestone in medical history that was achieved when surgeons performed a delicate operation to repair a valve in a patient’s heart. During the procedure, the patient’s defective valve was trimmed and reconstructed. The operation was remarkable not because of what it accomplished—many thousands of patients have undergone heart-valve repair—but because of how it was done. During much of the complex procedure, the surgeon’s hands never entered or touched the patient’s body. Master-slave manipulators and telerobots are a special breed of robot-one in which the human is directly in the control loop commanding the robot’s actions. Telerobots are a modern version of master-slave manipulator technology, which has been used successfully in critical applications for more than four decades. The Intuitive system and others like it mark the beginning of a potentially huge wave of surgical applications for telerobots. With the assistance of surgical telerobots, surgeons will extend their healing skills to places within the body that are currently out of reach.
Impaired development of human Th1 cells in patients with deficient expression of STAT4 (2009)

IL-12 activates STAT4, which is a critical regulator of inflammation and T helper type I (Th1) lineage development in murine systems. The requirement for STAT4 in the generation of human Th1 cells has not been examined thoroughly. Compared with control Th1 cultures, expression of the Th1 genes IFNgamma, IL-12Rbeta2, and TNFalpha is greatly reduced in Th1 cultures of CD4 T cells isolated from lymphoma patients after autologous stem cell transplantation who have acquired STAT4 deficiency. Moreover, IL-4 and IL-5 production is increased in patient Th1 cultures though there are no defects in the development of Th2 cells. Reconstitution of STAT4 in patient T cells allowed recovery of IFNgamma and IL-12Rbeta2 expression, whereas ectopic expression of IL-12Rbeta2 did not rescue STAT4 expression, and increased IFNgamma production only to levels intermediate between control and patient samples. These results demonstrate that, as in murine systems, STAT4 is required for optimal human Th1 lineage development.
A scheduling scheme for the heterogeneous multimedia services in mobile broadband systems (2002)

As the development of the third generation mobile systems proceed, researchers are turning their attention to the integration of multimedia service under the wired and wireless environments. One challenging requirement is that mobile systems convey information of diverse types including voice, computer data, facsimile, and video data which has different quality of service constraints. Basically, a centralized control scheme for a coordinated multiple access is required in order to efficiently accommodate multimedia traffic on wireless links. The proposed scheme dynamically scheduling the mobile terminal which has data to transfer according to the service types at the base station. In this scheme, we select the key point of the quality of service as the time constraint between transmission intervals. Simulation results show that exploiting the information on the timestamp in the transmission terminal scheduling is a good solution in respect to delay sensitive services.
High Performance Amorphous Metallated π-Conjugated Polymers for Field-Effect Transistors and Polymer Solar Cells (2008)

Both high charge carrier mobility in OFETs and high power conversion efficiency in solar cells were demonstrated in a series of amorphous metallated π-conjugated polymers. The best solar cell performance is based on a high field-effect mobility (μh = 1.0 × 10−2 cm2 V−1 s−1) polymer blended with PC71BM leading to a power conversion efficiency of PCE = 4.13%.
Effect of Atmosphere and Temperature Treatment on Leoanardite for Increasing Humic Acid Yield (2018)

ABSTRACT Humic acid is a complex molecule including carboxylic, phenolic, alcoholic, and carbonyl fractions in its structure. Addition of this complex structure in soil improves its fertility by increasing its porosity along with water/nutrient capacity. Although naturally exists in soil, humic acid must be added to soil externally in regions with low soil fertility. The present study was conducted to provide insight on improving the efficiency of humic acid yield during its extraction. Leonardite was selected as raw material due to its higher amounts of humic acid compared to low rank coals, soil, agricultural crop residues, compost, sewage sludge, landfill leachate and animal waste. Leonardite was treated at 200, 300 and 400°C, in the presence of air and nitrogen atmospheres and humic acid was extracted from this treated samples. Compared to untreated leonardite, an improvement in humic acid efficiency was achieved for samples treated at 200°C, in the presence of nitrogen atmosphere. The reasons of this distinct behavior in the presence of nitrogen was elaborated with elemental, FT-IR, UV-Vis and XRD analyses.
Magnetic compensation of gravity forces in liquid/gas mixtures: surpassing intrinsic limitations of a superconducting magnet by using ferromagnetic inserts (2005)

Magnetic compensation of gravity forces, similar to the space conditions of “microgravity”, needs the production of a uniform magnetic force field. We derive here a basic mathematical result that shows the impossibility to establish exact gravity compensation in a finite volume. The imperfection of compensation can be, however, quantified and a relation is derived between homogeneity accuracy and compensation volume in a cylindrical symmetry. We study how the use of inserts made of saturated ferromagnetic materials can modify the homogeneity of magnetic force field. In order to illustrate this result, an iron insert has been numerically calculated for the particular case of gravity compensation of H2 in a 10 T superconducting coil. An experimental test has been carried out on a H2 vapour bubble very close to its gas-liquid critical point. Near the critical point the gas-liquid interfacial tension is vanishing, then any bubble deformation from the ideal spherical shape reveals the non-homogeneities in the magnetic compensation force.
Field Trial of WDM-OTDM Transmultiplexing employing Photonic Switch Fabric-based Buffer-less Bit-interleaved Data Grooming and All-Optical Regeneration (2009)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text G. Zarris, F. Parmigiani, E. Hugues-Salas, R. Weerasuriya, D. Hillerkuss, N. A. Gonzalez, M. Spyropoulou, P. Vorreau, R. Morais, S. K. Ibrahim, D. Klonidis, P. Petropoulos, A. D. Ellis, P. Monteiro, A. Tzanakaki, D. Richardson, I. Tomkos, R. Bonk, W. Freude, J. Leuthold, and D. Simeonidou, "Field Trial of WDM-OTDM Transmultiplexing employing Photonic Switch Fabric-based Buffer-less Bit-interleaved Data Grooming and All-Optical Regeneration," in Optical Fiber Communication Conference and National Fiber Optic Engineers Conference, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper PDPC10. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
What Are Realistic Expectations to Become Free of Overactive Bladder Symptoms? Experience from Non-interventional Studies with Propiverine (2022)

These data provide an evidence base for the counselling of patients with overactive bladder on realistic expectations of treatment outcomes. We propose that realistic expectations can lead to greater long-term adherence.
Thermal plasticity of metabolic rates linked to life-history traits and foraging behaviour in a parasitic wasp (2010)

1. Ectotherm organisms are known to be plastic in their responses to temperature. Individuals developing at low temperatures usually achieve a larger body size through a lower growth rate (‘Temperature Size Rule’, TSR) and, when compared at the adult stage in a common environment, have a higher metabolic rate than those developing at higher temperatures (‘Thermal Compensation Hypothesis’, TCH). 2. For such plastic individuals, increased metabolic rate may incur a switch in the energetic allocation between competing functions such as maintenance and reproduction. Consequently, this differential allocation is expected to affect the animals’ foraging behaviour. 3. This study investigates the effect of developmental temperatures and temperature experienced as adults on metabolic rate, life-history traits and foraging behaviour of females of the parasitic wasp Aphidius rhopalosiphi. For this purpose, individuals were reared at 10, 15, 20 and 25 °C. At emergence, we checked if their traits followed the TSR. We then placed all females in a common environment (20 °C), measured their metabolic rate and examined if a switch of energetic allocation occurred between longevity and/or egg load. Finally, we examined the physiological consequences of temperature on their host patch exploitation behaviour. 4. First, our results showed that traits at emergence followed the TSR. Second, metabolic rates of individuals developing at low temperatures were higher, following the TCH and leading to a reduction in longevity but an increase in oviposition rate. Finally, we showed that patch exploitation behaviours conformed to the predictions of state-dependent foraging models and that hosts which were not exposed to different temperatures, modified their behavioural defences. 5. To our knowledge, this is the first study to demonstrate adaptive thermal developmental plasticity in metabolic rates and its consequences on thermal plasticity of life-history traits and foraging behaviour in ectotherms.
Multitree-like Graph Layering Crossing Optimization (2019)

We improve a method of multitree-like graph visualization using a spanning tree-driven layout technique with constraints specified by layers and the ordering of groups of nodes within layers. We propose a new method of how the order of subtrees selected by the driving spanning tree can be derived from the actual edge crossings. Such a subtree order leads to additional decreasing of total edge crossings from 1% to 50%. This depends on the shape of the processed graph, ranging from a pure tree to a general acyclic graph. Our achievements are demonstrated using several datasets containing up to millions of people, species, or services. The proposed subtree ordering method of layered graphs that are similar to acyclic multitrees retains the generating of acceptable layouts in almost linear time.
Vasopressin responses to corticotropin-releasing factor and hypertonicity after truncal vagotomy in dogs (1996)

Infusion of corticotropin-releasing factor (CRF) augments the plasma vasopressin response to infusion of hypertonic saline in conscious dogs. Furthermore, afferent vagal nerve input from the abdomen is involved in the control of vasopressin release and may be altered by CRF. The purpose of the present study was to characterize the effect of CRF on the vasopressin response to hypertonic saline and to determine if it is mediated by afferent input carried from the abdominal vagus. Conscious male dogs (n = 5) underwent infusion of isotonic saline (vehicle), CRF (10 or 20 ng.kg-1.min-1), hypertonic saline (0.2 mmol.kg-1.min-1), or the combination of CRF and hypertonic saline. Hypertonic saline increased plasma sodium from 147 +/- 1 to 153 +/- 1 meq/1 and plasma vasopressin from 2.5 +/- 0.1 to 5.8 +/- 0.4 pg/ml. CRF infusion alone had no effect on plasma vasopressin. The addition of 10 or 20 ng.kg-1.min-1 CRF augmented the vasopressin response to hypertonic saline to 7.7 +/- 1.7 and 6.9 +/- 0.3 pg/ml, respectively. Truncal vagotomy did not attenuate the vasopressin response to hypertonic saline with or without CRF infusion. We conclude that CRF augments the vasopressin response to hypertonic saline and that this effect is not mediated via afferents from the abdominal vagus.