Polymerase Chain Reaction for Diagnosis of HIV Infection (1997)

Letters1 May 1997Polymerase Chain Reaction for Diagnosis of HIV InfectionArthur E. Brown, MD, MPH, Merlin Robb, MD, and Francine McCutchan, PhDArthur E. Brown, MD, MPHWalter Reed Army Institute of Research, Washington, DC 20307Henry M. Jackson Foundation, Rockville, MD 20850Search for more papers by this author, Merlin Robb, MDWalter Reed Army Institute of Research, Washington, DC 20307Henry M. Jackson Foundation, Rockville, MD 20850Search for more papers by this author, and Francine McCutchan, PhDWalter Reed Army Institute of Research, Washington, DC 20307Henry M. Jackson Foundation, Rockville, MD 20850Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-126-9-199705010-00017 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail TO THE EDITOR:The recent paper by Owens and colleagues [1] presents a meta-analysis of the performance of polymerase chain reaction (PCR) for the diagnosis of human immunodeficiency virus type 1 (HIV-1) infection. The authors do not comment on either the genetic sequences to which the PCR primers are targeted or the genetic differences documented among the subtypes of HIV-1. Variability in PCR across subtypes was the subject of a recent UNAIDS meeting, at which the need for further assay development was identified [2].At the Walter Reed Army Institute of Research, a DNA PCR assay is used to supplement ...References1. Owens DK, Holodniy M, Garber AM, Scott J, Sonnad S, Moses L, et al. Polymerase chain reaction for the diagnosis of HIV infection in adults. A meta-analysis with recommendations for clinical practice and study design. Ann Intern Med. 1996; 124:803-15. Google Scholar2. Anderson RM, Schwartlander B, McCutchan F, Hu D. Implications of genetic variability in HIV for epidemiology and public health. Lancet. 1996; 347:1778-9. Google Scholar Author, Article, and Disclosure InformationAuthors: Arthur E. Brown, MD, MPH; Merlin Robb, MD; Francine McCutchan, PhDAffiliations: Walter Reed Army Institute of Research, Washington, DC 20307Henry M. Jackson Foundation, Rockville, MD 20850 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byHuman immunodeficiency virus type 1 recombinant B/G subtypes circulating in Coimbra, PortugalHigh prevalence of HIV-1 subtype G and natural polymorphisms at the protease gene among HIV-infected immigrants in MadridEarlier Detection of Human Immunodeficiency Virus Type 1 p24 Antigen and Immunoglobulin G and M Antibodies to p17 Antigen in Seroconversion Serum Panels by Immune Complex Transfer Enzyme ImmunoassaysRecombinant Human Immunodeficiency Viruses Type 1 Circulating in SpainHuman immunodeficiency viruses type 1 subtypes circulating in SpainDiversity of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein in San Francisco Men's Health Study Participants 1 May 1997Volume 126, Issue 9Page: 739KeywordsAlgorithmsDNAGeneticsHIVHIV infectionsHIV-1Nucleic acidsPolymerase chain reaction ePublished: 15 August 2000 Issue Published: 1 May 1997 Copyright & PermissionsCopyright © 1997 by American College of Physicians. All Rights Reserved.PDF downloadLoading ...