gebhart/patrepeval huggingface dataset:
Evaluation of probiotics for inhibiting hyperproliferation and inflammation relevant to psoriasis in vitro (2021)

Due to the complexity of psoriasis, people have been searching for better treatment methods, and probiotics therapy is gaining acceptance. In this study, tumor necrosis factor-α (TNF-α) was used to stimulate abnormal proliferation of HaCaT cells, and the inhibitory effects of potential probiotics supernatants on hyperproliferation were compared. Among 102 strains, two with better suppressive effects, Bifidobacterium animalis CCFM1148 and Lactobacillus paracasei CCFM1147, were selected, whereas the other two were selected as control strains. Based on RNA-seq results, CCFM1148 and CCFM1147 inhibited TNF-α-induced activation of the nuclear factor kappa-B (NF-κB) signaling pathway and altered the cellular response to interleukin (IL)-8, while the two control strains did not. It was speculated that the inhibitory effect of strains was through the inhibiting of the activation of the NF-κB and the release of proinflammatory cytokines, and subsequent experiments confirmed this. Collectively, these findings suggest that CCFM1148 and CCFM1147 have potential to ameliorate psoriasis.
13C‐NMR studies of commercial and partially self‐condensed hexakis(methoxymethyl)melamine (HMMM) resins (1996)

13C-nuclear magnetic resonance (NMR) spectroscopy was employed to study the linkages in three commercial fully methylocated melamine-formaldehyde (HMMM) resins and their partially self-condensed resins by acid catalysis, in order to probe the structures linking melamine units. Distortionless enhancement by polarization transfer (DEPT) experiments show that the partially self-condensed resins contain both methylene ether and methylene linkages between melamine units. The extent of these linkages were estimated by quantitative 13C-NMR spectroscopy using inverse gated decoupling techniques. The results show that the ratios of methylene ether and methylene linkages to the triazine moiety vary from resin to resin. © 1996 John Wiley & Sons, Inc.
Morphologic and biochemical analysis of the intracellular trafficking of the Alzheimer beta/A4 amyloid precursor protein (1994)

Abnormal metabolic processing of the beta/A4 amyloid precursor protein (APP) has been implicated in the pathogenesis of Alzheimer disease. Several aspects of normal APP processing have been elucidated, but the precise cellular trafficking of APP remains unclear. To investigate APP trafficking pathways further, we have examined the subcellular distribution of APP in rat brain tissue and a variety of cultured cell types, and correlated this distribution with the biochemical processing of APP. In immunofluorescence microscopy of rat brain sections, APP immunoreactivity was concentrated in the Golgi complex and in proximal axon segments. In addition, a lower level of punctate fluorescence was visible throughout the neuropil. By immunoelectron microscopy of rat brain tissue fragments, APP was found associated with Golgi elements and with medium-sized, invaginated vesicles in both axons and dendrites. Prominent localization of APP to the Golgi complex was also found in primary cultures of rat hippocampal neurons and in non-neuronal cell lines. When cultured cells were treated with brefeldin A (BFA), APP immunoreactivity changed from a Golgi-like to an endoplasmic reticulum-like distribution. No APP was detected in the BFA-induced reticulum identified by the transferrin receptor, indicating that concentration of APP in the Golgi does not reflect recycling between the trans-Golgi network and early endosomal system. In immunoblots of BFA-treated cells, there was an accumulation of full-length APP and inhibition of APP secretory processing. Treatment with phorbol ester resulted in a marked elevation of APP secretion, but no obvious redistribution of APP immunoreactivity was apparent at the light microscope level. The lysosomotropic drug chloroquine induced accumulation of APP in cell lysates, as seen by immunoblotting. Immunofluorescence microscopy of chloroquine-treated cells demonstrated a colocalization of APP with the lysosomal marker Igp 120, whereas no colocalization was seen in untreated cells. Taken together, these results support a scheme in which APP is concentrated in the Golgi complex as it travels through the central vacuolar system en route to the plasma membrane for secretion of its amino-terminal domain and/or to lysosomes for degradation.
Branch specificity of bovine colostrum and calf thymus UDP-Gal: N-acetylglucosaminide beta-1,4-galactosyltransferase. (1984)

The branch specificity of bovine colostrum and calf thymus UDP-Gal:N-acetylglucosaminide beta-1----4-galactosyltransferase toward several branched oligosaccharides, which form part of the complex-type N-glycans of glycoproteins, was investigated. A novel method was used based on acetolysis of the bi[14C,3H] galactosylated oligosaccharide products formed by the enzymes in vitro and analysis of the acetolysis fragments by high-pressure liquid chromatography. It could be established that the galactosylation of different oligosaccharide branches occurred in a preferred order. No difference in branch specificity was observed between the soluble bovine colostrum galactosyltransferase and the enzyme that had been solubilized from calf thymus membranes. A preferential pathway for the biosynthesis of bisialylated biantennary glycans is proposed.
The Power Images in the Style of a Leader: Expressive Characteristics for the Power Images on the Portrait of Henry VIII (2013)

The purpose of this study is to examine the power images in the style of a leader: through the focus on the portrait of Henry VIII. Study methods on the literature concerning viewpoints of power and image in the early modern age and the subjects of power are being used for theoretical backgrounds; and the visual data from National Portrait Gallery, British Library and the internet are used for exploratory studies. The results of this study are as follows. First, from the style of Henry VIII who represented the image of political power, the modern political phenomenon can be defined. Such phenomenon which displays images of political leaders from the study indicate an effective strategy. Second, the religious, a diplomatic representation for the style of Henry VIII powers, such as costume style and somatic visual images, represents the dignity and power by delivering visible internal attributes. Thus, the role model recognized by the society and the public's perception of leadership style affects the acknowledgement and belief by the people.
Delivery of CR2-fH Using AAV Vector Therapy as Treatment Strategy in the Mouse Model of Choroidal Neovascularization (2017)

Complement activation plays a significant role in age-related macular degeneration (AMD) pathogenesis, and polymorphisms interfering with factor H (fH) function, a complement alternative pathway (AP) inhibitor, are associated with increased AMD risk. We have previously validated an AP inhibitor, a fusion protein consisting of a complement receptor 2 fragment linked to the inhibitory domain of fH (CR2-fH) as an efficacious treatment for choroidal neovascularization (CNV) when delivered intravenously. Here we tested an alternative approach of AAV-mediated delivery (AAV5-VMD2-CR2-fH or AAV5-VMD2-mCherry) using subretinal delivery in C57BL/6J mice. Secretion of CR2-fH was confirmed in polarized retinal pigment epithelium (RPE) cells. A safe concentration of AAV5-VMD2-CR2-fH was identified using electroretinography, optical coherence tomography (OCT), RPE morphology, and antibody profiling. One month after gene delivery, CNV was induced using argon laser photocoagulation. OCT assessment demonstrated reduced CNV with AAV5-VMD2-CR2-fH administration. Bioavailability studies revealed that gene-therapy delivered similar levels of CR2-fH to the RPE/choroid as treatment by intravenous injections, and C3a ELISA verified reduced CNV-associated ocular C3a production. These results contribute to existing data illustrating the importance of the AP of complement in CNV development and its potential role in AMD treatment. Demonstration of AAV-vector efficacy opens new avenues for the development of treatment strategies.
RF immunity investigations of linear DC current regulators (2015)

This paper studies and compares the RF immunity of DC current regulators against electromagnetic interferences (EMIs). The effect of EMI on the performance of a classic topology is analyzed. It turns out, especially the regulators output is very sensitive against EMI since disturbance levels in the millivolt range cause serious malfunctions. Subsequently the paper introduces a structured approach to the design of EMI resistant regulators. RF immunity measurements on IC level (DPI) and on system level (BCI) proof the superior EMC performance of the resisting topologies over the classic regulator, while the electrical characteristics and the area of the circuits are the same. The circuits have been designed using a HV- BiCMOS technology for automotive applications.
Maximum likelihood of phylogenetic networks (2006)

Implementation of the criteria as well as heuristics and hardness proofs are available from the authors upon request. Hardness proofs can also be downloaded at http://www.cs.tau.ac.il/~tamirtul/MLNET/Supp-ML.pdf
Optical Performance of the π Cell Compensated with a Negative-Birefringence Film and an A-plate (1999)

The π cell is known to be a fast and wide-viewing-angle liquid-crystal-display mode due to its unique director configuration. To compensate the viewing angle dependence of the positive birefringence of the π cell, we formulated the idea of using a negative-birefringence structure which has a mirror image of the director configuration of the π cell. Our calculation showed that this combination provides an excellent viewing angle, which was experimentally confirmed by applying a fabricated optical compensation film composed of a discotic layer and an aromatic polyimide layer, both of which have negative birefringence, to a test π cell. An extended Poincaré sphere analysis explained the optical effect of the compensation film and revealed that polarizers are responsible for the low contrast ratio at oblique angles. A complete viewing angle was achieved by adding a positive a-plate which suppresses light leakage at oblique incidence. Color shift upon viewing from oblique angles was also examined. Our experiment showed that the color shift of the π cell is much less than that of the twisted-nematic liquid-crystal display compensated with a similar negative-birefringence film.
Implementation issues for on-chip learning with analogue VLSI MLPS (1995)

Microelectronic neural network technology has become sufficiently mature over the past few years that reliable performance can now be obtained from VLSI circuits under carefully controlled conditions. The use of analogue VLSI allows low power, low cost and area efficient hardware realisations which can perform the computationally intensive feed-forward operation of neural networks at high speed. These factors, coupled with the ability to interface directly with the analogue world, make real-time applications a possibility. This paper attempts to address some of the issues concerning in-situ learning with analogue VLSI multilayer perceptron (MLP) networks. We consider the modes used to train analogue neural networks, study weight storage and circuit precision issues, and identify the most promising training algorithms. We then make some conclusions based on results from analogue VLSI chips that we have designed, built and successfully tested.
Development and implementation of an 84‐channel matrix gradient coil (2017)

A shielded matrix gradient coil setup capable of generating encoding fields in a highly-flexible manner was designed and implemented. The presented setup is expected to serve as a basis for validating novel imaging techniques that rely on nonlinear spatial encoding fields. Magn Reson Med 79:1181-1191, 2018. © 2017 International Society for Magnetic Resonance in Medicine.
Fluorophore-Assisted Light Inactivation of Calmodulin Involves Singlet-Oxygen Mediated Cross-Linking and Methionine Oxidation (2006)

Fluorophore-assisted light inactivation (FALI) permits the targeted inactivation of tagged proteins and, when used with cell-permeable multiuse affinity probes (MAPs), offers important advantages in identifying physiological function, because targeted protein inactivation is possible with spatial and temporal control. However, reliable applications of FALI, also known as chromophore-assisted light inactivation (CALI) with fluorescein derivatives, have been limited by lack of mechanistic information regarding target protein sensitivity. To permit the rational inactivation of targeted proteins, we have identified the oxidizing species and the susceptibility of specific amino acids to modification using the calcium regulatory protein calmodulin (CaM) that, like many essential proteins, regulates signal transduction through the reversible association with a large number of target proteins. Following the covalent and rigid attachment of 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein (FlAsH) to helix A, we have identified light-dependent oxidative modifications of endogenous methionines to their corresponding methionine sulfoxides. Initial rates of methionine oxidation correlate with surface accessibility and are insensitive to the distance between the bound fluorophore and individual methionines, which vary between approximately 7 and 40 A. In addition, we observed a loss of histidines, as well as zero-length cross-linking with binding partners corresponding to the CaM-binding sites of smooth myosin light chain kinase and ryanodine receptor. Our results provide a rationale for proteomic screens using FALI to inhibit the function of many signaling proteins, which, like CaM, commonly present methionines at binding interfaces.
ChemInform Abstract: THE REACTION OF ALKYLIDENE YLIDES WITH FLUOROOLEFINS (1976)

Abstract Das Phosphoniumylid (I), das kein α‐Wasserstoffatom aufweist, reagiert mit Trifluorchloräthylen (II) zum allylischen Phosphoniumsalz (III), aus dem durch Hydrolyse in quantitativer Ausbeute das Olefin (IV) erhältlich ist.
Thin Film Deposition Using Energetic Ions (2010)

One important recent trend in deposition technology is the continuous expansion of available processes towards higher ion assistance with the subsequent beneficial effects to film properties. Nowadays, a multitude of processes, including laser ablation and deposition, vacuum arc deposition, ion assisted deposition, high power impulse magnetron sputtering and plasma immersion ion implantation, are available. However, there are obstacles to overcome in all technologies, including line-of-sight processes, particle contaminations and low growth rates, which lead to ongoing process refinements and development of new methods. Concerning the deposited thin films, control of energetic ion bombardment leads to improved adhesion, reduced substrate temperatures, control of intrinsic stress within the films as well as adjustment of surface texture, phase formation and nanotopography. This review illustrates recent trends for both areas; plasma process and solid state surface processes.
Learning from multiple demonstrations using trajectory-aware non-rigid registration with applications to deformable object manipulation (2015)

Learning from demonstration by means of non-rigid point cloud registration is an effective tool for learning to manipulate a wide range of deformable objects. However, most methods that use non-rigid registration to transfer demonstrated trajectories assume that the test and demonstration scene are structurally very similar, with any variation explained by a non-linear transformation. In real-world tasks with clutter and distractor objects, this assumption is unrealistic. In this work, we show that a trajectory-aware non-rigid registration method that uses multiple demonstrations to focus the registration process on points that are relevant to the task can effectively handle significantly greater visual variation than prior methods that are not trajectory-aware. We demonstrate that this approach achieves superior generalization on several challenging tasks, including towel folding and grasping objects in a box containing irrelevant distractors.
Distinct voltage-dependent regulation of a heart-delayed IK by protein kinases A and C (1991)

We have investigated the effects of stimulation of adenosine 3',5'-cyclic monophosphate-dependent protein kinase (protein kinase A) and Ca(2+)-diacylglycerol-dependent protein kinase (protein kinase C) on the delayed rectifier K+ current (IK) in guinea pig ventricular cells using a whole cell arrangement of the patch-clamp procedure. Stimulation of either protein kinase C or A resulted in enhanced IK activity. Augmentation of IK observed during stimulation of protein kinase A occurred in a markedly voltage-dependent manner, with the largest increases occurring at potentials near the threshold for IK activation. Enhancement of IK during stimulation of protein kinase C followed a different pattern, with minimal effects of the enzyme near IK threshold. Neither protein kinase A nor C altered the kinetics of IK activation, although both kinases slightly changed the kinetics of deactivation. Both kinases increased IK maximal conductance, but the effects of each kinase on the voltage-dependence of activation differed. Protein kinase A shifted IK activation toward more negative voltages but did not affect the slope of the activation curve. Protein kinase C, in contrast, changed the slope of the IK activation curve, with only a small effect on the half-maximal voltage of activation. These contrasting effects on the voltage dependence of IK activation are consistent with actions of the kinases at distinct sites on or near the IK channel protein.
A novel MCM interconnect test technique based on resonator principles and transmission line theory (2002)

This paper describes a new technique for detecting and diagnosing process-related interconnect faults in MCMs. The technique uses a single-ended probe with a resonator and a waveform generator: A complete fault diagnosis is possible through measurement of magnitude and phase responses.
Helicobacter pylori Infection in the Cat: Evaluation of Gastric Colonization, Inflammation and Function (2001)

The pattern of colonization and the mucosal inflammatory response in cats with naturally acquired H. pylori are broadly similar to those in infected people, particularly children, and non-human primates. The upregulation of IL-8 in infected cats was independent of cagA and picB. Our findings argue against a direct acid-suppressing effect of H. pylori on the gastric secretory-axis in chronically infected cats.
Liquid–liquid extraction of biomolecules: an overview and update of the main techniques (2007)

Abstract Two‐phase systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental work on the use of aqueous two‐phase polymer systems, two‐phase aqueous micellar systems and reversed micellar systems for the extraction/purification of biomolecules. The experimental partitioning behavior of different biomolecules is reviewed and new results with nisin and lipopolysaccharides are presented. An extensive description of each system is presented, based on the biomolecules behavior and the tools employed to improve their extraction and purification. Copyright © 2007 Society of Chemical Industry
Novas estratégias de modelagem (2017)

II.3. Novas estratégias de modelagemDepois dos modelos propostos por Peirce e especialistas, apresentados nas seções anteriores, propomos estratégias de mode-
Effect of Topical Application of Hydro-Ethanolic Extract of Acacia nilotica Pods on Second-Degree Cutaneous Infected Burns (2015)

Acacia nilotica is a medicinal plant used traditionally in Togo to treat various diseases including burn wounds. The aim of this study was to evaluate experimentally Acacia nilotica burn wound healing effect.Cutaneous burn wounds were symmetrically caused on depilated areas of rat skin through contact with an aluminum bar (r = 10 mm), preheated at 80°C for 30 sec. Five groups of animal were constituted and each group contained 8 mice. Four groups of animal’s burn wounds were infected by Staphylococcus aureus. One group burn wound is uninfected and serves as negative control. Burn wounds were assessed by planimetry and histological parameters of healing. Twelve days after burn wound induction, wound contraction in the uninfected groups (negative control) was 19.9% for topical application against -2.43% for infected control. In the infected groups treated with extract, wound contraction was generally stimulates. Histological examination showed granulated tissue developing over the wounds treated with the extract of A. nilotica at 2.5% and 5% where the proliferation of fibroblasts and neo-blood vessels was very marked.In conclusion, A. nilotica pod contents tannin, flavonoids, alkaloids and protein reduces DPPH solution and significantly accelerates wound healing of burns, and this is the case even if wounds are infected with 109CFU/mL of S. aureus.
ChemInform Abstract: PHOTO‐INDUZIERTE CYCLISIERUNG VON CIS,CIS‐2,8‐CYCLONONADIENON UEBER DAS CIS,TRANS‐ISOMERE (1971)

Abstract cis,cis‐2,8‐Cyclononadienon (I) liefert bei der Bestrahlung in Eisessig die bicy schen Reaktionsprodukte (IIIa) und (IVa), mit Methanol die entsprechenden Äther (IIIb) bzw. (IVb).
Interaction Approach to Contingency Theory: A Reexamination of Child's Manufacturing Subsample (1993)

Abstract A comprehensive data set was reanalyzed using multiple regression models. Alternative sets of models were developed for each of six performance variables. The results indicated that five of the six performance outcomes had significant predictors. Four of these five models contained significant interaction terms. It is concluded that even though the two methods produce different results, further investigation of the interaction approach to contingency theory within industrial sectors is warranted. Résumé Un vaste ensemble de données a été analysé à nouveau à l'aide de multiples modèles régressifs. Des ensembles de modèles alternatifs ont été développés pour chacune des six performances changeantes. Les résultats étaient tels que cinq sur six d'entre eux pouvaient être prédits de façon évidente; quatre de ces cinq modèles comprenaient des interactions significatives. En conclusion, bien que les deux méthodes produisent des résultats différents, une recherche plus poussée de l'approche d'interaction au sein des secteurs industriels est justifiée concernant cette théorie d'éventualité.
Interactive Multimedia Services for Consumers and Businesses (1995)

The emergence of broadband networks for residential and business customers enables a wide range of new interactive services. For consumers, these new services might include movies on demand, networked multi-player video games, and interactive shopping via TV. Business applications might include broadcast-quality video teleconferencing, distance learning, and telecommuting. This paper presents the technical issues and infrastructure involved in delivering several of these services, along with some of the lessons learned and insights gained from AT&T's early interactive broadband services trials.
Los riegos profesionales: accidentabilidad laboral con enfoque jurídico, económico y social (2013)

Analisis juridico, social y economico, referido a la accidentabilidad laboral en Nicaragua, partiendo de un breve antecedente del derecho laboral y de la seguridad social; realizando para ello un analisis de los datos estadisticos, publicados por el Instituto Nicaraguense de Seguridad Social, en los periodos 2003-2011.
Effects of Prostaglandin F 2α on Adipocyte Biology Relevant to Graves' Orbitopathy (2013)

The results demonstrate that PGF2α significantly reduces proliferation and adipogenesis and that human OFs are more sensitive to its effects than 3T3-L1. Consequently, PGF2α could be effective in the treatment of GO.