gebhart/patrepeval huggingface dataset:
Characterization of a novel monoclonal antibody that senses nitric oxide‐dependent activation of soluble guanylate cyclase (1999)

Two monoclonal antibodies (mAbs) against bovine lung soluble guanylate cyclase (sGC) were prepared and characterized. mAb 3221 recognized both the alpha- and beta-subunits of sGC and had greater binding affinity to the enzyme in the presence of NO. mAb 28131 recognized only the beta-subunit and its affinity did not change with NO. Neither mAb cross-reacted with particulate GC. Cultured Purkinje cells from rats were treated with S-nitroso-N-acetylpenicillamine, an NO donor, and examined by immunocytochemical methods. The immunoreactivity associated with mAb 3221 increased with the cGMP content in a crude extract of cerebellum and the NO2 generated in the culture medium increased.
Textured or Smooth Implants for Submuscular Breast Augmentation: A Controlled Study (1996)

Capsular contracture consistently has been the most frequently noted complication of submuscular and subglandular breast augmentation. The etiology of this complication is still unknown, although silicone bleed, hematoma, infection, foreign bodies, and surgical trauma have been implicated. In this prospective, double-blind study, 61 women undergoing submuscular breast augmentation were randomized between Dow Corning textured and smooth-walled silicone gel implants. Any consequent capsular contracture was assessed by an independent plastic surgeon and also by the patients themselves. Objective evaluation was made by applanation tonometry. It was found that depending on doctors, patients, and objective method used, 3 to 9 percent grade III and IV encapsulation followed submuscular augmentation with textured implants and 10 to 20 percent with smooth-walled implants after 1 year. The differences were significant according to both patient assessment and applanation tonometry but not according to the physicians' evaluations. There was no correlation of capsular contracture with the age of the patient, duration of the operation, or degree of blood loss. There was a small but inconclusive difference in capsular contracture rate that favored the placement of textured rather than smooth implants in the submuscular pocket.
On Passive Symmetrical Two-Ports, Impedance Conversion and Power Transfer (2010)

The theory of linear passive symmetrical two-ports is reviewed. Many results of practical interest can be derived from the very compact analytical basis of this model. However, such derivations are scattered in the literature. In addition, the demonstrations do not always take advantage of the generality that can be obtained by avoiding implementations built on particular applications. A complete, self consistent analysis is presented here. Attention is focused on power transfer and the relations between the impedances at each port. The specific case of transmission lines is finally addressed.
IKKβ and Phosphatidylinositol 3-Kinase/Akt Participate in Non-pathogenic Gram-negative Enteric Bacteria-induced RelA Phosphorylation and NF-κB Activation in Both Primary and Intestinal Epithelial Cell Lines (2021)

Pathogenic and enteroinvasive bacteria have been shown to trigger the I kappa B/NF-kappa B transcriptional system and proinflammatory gene expression in epithelial cells. In this study, we investigated the molecular mechanism of the commensal Gram-negative Bacteroides vulgatus-induced NF-kappa B signal transduction in intestinal epithelial cells (IEC). We report that B. vulgatus induced interleukin-1 receptor-associated kinase-1 degradation, I kappa B alpha phosphorylation/degradation, RelA and Akt phosphorylation, as well as NF-kappa B DNA binding and NF-kappa B transcriptional activity in rat non-transformed IEC-6 cells. B. vulgatus- but not interleukin-1 beta-mediated NF-kappa B transcriptional activity was inhibited by dominant negative (dn) toll-like receptor 4. Of importance, B. vulgatus induced I kappa B alpha phosphorylation/degradation and IKK alpha/beta and RelA phosphorylation in primary IEC derived from germ-free or mono-associated HLA-B27 transgenic and wild type rats, demonstrating the physiological relevance of non-pathogenic bacterial signaling in IEC. Adenoviral delivery of dn IKK beta or treatment with wortmannin inhibited B. vulgatus-induced endogenous RelA Ser-536 and GST-p65TAD (Ser-529/Ser-536) phosphorylation as well as NF-kappa B transcriptional activity in IEC-6 cells, suggesting a critical role of IKK beta and phosphatidylinositol 3-kinase/Akt in bacteria-induced RelA phosphorylation and NF-kappa B activation. Interestingly, B. vulgatus-induced I kappa B alpha degradation and NF-kappa B transcriptional activity in IEC transwell cultures were inhibited in the presence of lymphocytes. We propose that non-pathogenic B. vulgatus activates the NF-kappa B signaling pathway through both I kappa B degradation and RelA phosphorylation but that immune cells mediate tolerance of IEC to this commensal bacteria.
Exclusive self-aligned β-phase PVDF films with abnormal piezoelectric coefficient prepared via phase inversion (2015)

Self-polarised poly(vinylidene fluoride), (PVDF), films were prepared via a facile phase-inversion technique wherein the polymorphism of the films was controlled from exclusive α- (>90%) to β-phase (>98%) by simply varying the quenching temperature from 100 °C to -20 °C, respectively. At low temperatures, the β-phase crystallites were found to be self-aligned, with the PVDF thin films possessing a high piezoelectric coefficient of up to -49.6 pm V(-1). The extraordinarily high β-phase and piezoelectric coefficient of these PVDF films make them suitable for electroactive and energy harvesting applications.
Frequency of Scapholunate Ligament Injuries Associated with Distal Radius Shearing Fracture: Correlation of Fracture Patterns and Ligament Tear (2015)

Scapholunate ligament injury is an important risk factor associated with high-energy distal radius shearing fractures. To prevent these problems, temporary scapholunate joint fixation is a recommended treatment for grade 3 or 4 scapholunate injuries.
Molecular cloning and analysis of a HSP (heat shock protein) ‐ Like 42 kDa antigen gene of Mycoplasma hyopneumoniae (1997)

The recombinant clone expressing the 42 kDa protein (P42) of Mycoplasma hyopneumoniae in Escherichia coli was analyzed. The 4.4 kb HindIII-Xmal DNA fragment expressing the p42 gene product encodes three ORFs: p42 and p16 in the forwarding strand, p24 in the reverse strand. Sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with Mycoplasma genitalium HSP70 gene and 56% identities with Bacillus subtilis dnaK gene; p16 and p24 genes share 73% and 47% identities with Erysipelothrix rhusiopathiae dnaJ gene and Pseudomonas fluorescens uvrC gene, respectively. Further analysis demonstrated that P42 is indeed a heat shock protein and the monospecific antibodies against P42 can block the growth of Mycoplasma hyopneumoniae.
Te-As-Se glass microstructured optical fiber for the middle infrared (2009)

We present the first fabrication, to the best of our knowledge, of chalcogenide microstructured optical fibers in Te-As-Se glass, their optical characterization, and numerical simulations in the middle infrared. In a first fiber, numerical simulations exhibit a single-mode behavior at 3.39 and 9.3 microm, in good agreement with experimental near-field captures at 9.3 microm. The second fiber is not monomode between 3.39 and 9.3 microm, but the fundamental losses are 9 dB/m at 3.39 microm and 6 dB/m at 9.3 microm. The experimental mode field diameters are compared to the theoretical ones with a good accordance.
Risk analysis of hemorrhage in stereo‐electroencephalography procedures (2019)

Detailed examination of every postimplant CT reveals that the total hemorrhage rate appears higher than previously reported. Most of these hemorrhages are small and asymptomatic. Our grading system may be useful to risk stratify these hemorrhages and awaits prospective validation.
beta-Lapachone induced cell death in human hepatoma (HepA2) cells. (1998)

In present study we studied the cytotoxic effects of beta-lapachone, a potent anticancer drug, on the human hepatoma cell line (HepA2) under serum-free condition. Most cells died after 2 microM beta-lapachone addition at 48 hours. No apoptotic characteristics of DNA ladder was documented by agarose DNA electrophoresis. The blockage of cell cycle at S phase and unscheduled DNA synthesis were demonstrated by flow cytometric analysis and anti-bromodeoxyuridine immunocytochemistry. Ultrastructural observation showed that the swollen mitochondria, dilatation and vesiculation of rER and proliferation of peroxisome-like granules appeared within the cytoplasm of HepA2 cells following drug treatment. Using enzyme cytochemistry, both peroxidase and acid phosphatase activities but not catalase activity were localised in these peroxisome-like granules. Therefore, these results suggested that (a) beta-lapachone has a novel cytotoxic effect on human hepatoma cell; (2) beta-lapachone induces the interruption of the cell cycle and unscheduled DNA synthesis in HepA2 cells; and (3) beta-lapachone promotes the proliferation of peroxisome-like granules containing peroxidase and acid phosphatase activities without evidence of catalase activity in hepatoma cell line.
The Present Status of Application of the Plastic Concepts in Structural Design (1984)

The different approaches of recognizing plastic behavior in the design of steel structures are reviewed and the design requirements that assure successful utilization of this behavior in practice are described. Partial recognition of plastic behavior is now an important part of the allowable-stress design provisions. Continuous beams and low-rise building frames are routinely designed by the plastic method, which represents a full recognition of plastic response. For the multistory frames, because of the effect of overall instability, design must be based on the stability limit load, not the plastic limit load. This complicates significantly the design process and may have affected wider adoption of the method. Inelastic behavior, however, is extensively utilized in the design of both low- and high-rise structures resisting seismic ground shaking. Some recent advances in this area are described.
<title>Performance evaluation of a texture-based segmentation algorithm</title> (1991)

Texture segmentations are crucial components of many remote sensing, scene analysis, and object recognition systems. However, very little attention has been paid to the problem of performance evaluation in the numerous algorithms that have been proposed by the image understanding community. In this paper, a particular algorithm is introduced and its performance is evaluated in a systematic manner on a wide range of scene and scenarios. Both the algorithm and the methodology used in its evaluation have significance in numerous applications in the computer-based image understanding field.
Estimation of size at three sites on the face (2002)

The subjective size of steel balls was judged by nine subjects using the method of Absolute Magnitude Estimation. The balls were rolled (‘‘scripted’’) over three areas of the face (forehead, cheek, and lower lip) using the right index distal finger pad. The areas were stimulated under three conditions; (1) the subjects upon themselves (intra-active touch); (2) the subjects upon another person (interactive touch); and (3) another person upon the face of the subject (interactive touch). There were significant differences among the three facial sites in the intra-active condition. When another person’s face was scripted by the subject, there were no differences among the sites. The results were mixed when another scripted upon the faces of the subjects. The results are considered in terms of tissue mechanics, mode of stimulation and representation of the sites on the somatosensory cortex. [Work supported by NIH.]
Directly heated four-terminal phase change switches (2014)

Measured results of a four-terminal directly heated RF phase change switch are presented. Germanium telluride (GeTe) is used as the main resistance change element, connecting the RF input/output lines. The resistivity of GeTe is switched between the high-resistance amorphous-state value of several kΩ and low-resistance crystallized value of less than 3.9 Ω in less than 404 μsec using current pulses passing though GeTe. The loss of this ohmic switch at ON state is less than 0.5 dB with isolation of more than 18 dB at frequencies up to 20 GHz, indicating a cutoff frequency of > 4 THz. The switch active area is less than 5 μm × 20 μm with GeTe volume of only 1.5 μm × 15 μm × 0.25 μm. The results presented in this paper prove phase change switches are competitive contenders as low-loss and fast RF power switches.
Coexpression of phosphotyrosine-containing proteins, platelet-derived growth factor-B, and fibroblast growth factor-1 in situ in synovial tissues of patients with rheumatoid arthritis and Lewis rats with adjuvant or streptococcal cell wall arthritis. (1993)

Fibroblast growth factor (FGF)-1 and PDGF-B-like factors have been implicated in the pathobiology of RA and animal models of this disease. Since the receptors for FGF-1 and PDGF are tyrosine kinases, we examined the expression of tyrosine phosphorylated proteins (phosphotyrosine, P-Tyr) in synovial tissues from patients with RA and osteoarthritis (OA), and rats with streptococcal cell wall (SCW) and adjuvant arthritis (AA). Synovia from patients with RA and LEW/N rats with SCW and AA arthritis, in contrast to controls, stained intensely with anti-P-Tyr antibody. The staining colocalized with PDGF-B and FGF-1 staining. Comparative immunoblot analysis showed markedly enhanced expression of a 45-kD P-Tyr protein in the inflamed synovia. Treatment with physiological concentrations of dexamethasone suppressed both arthritis and P-Tyr expression in AA. P-Tyr was only transiently expressed in athymic nude Lewis rats and was not detected in relatively arthritis-resistant F344/N rats. These data suggest that (a) FGF-1 and PDGF-B-like factors are upregulated and may induce tyrosine phosphorylation of proteins in vivo in inflammatory joint diseases, (b) persistent high level P-Tyr expression is T lymphocyte dependent, correlates with disease severity, and is strain dependent in rats, (c) corticosteroids, in physiological concentrations, downregulate P-Tyr expression in these lesions.
Analyses Adjusting for Selective Crossover Show Improved Overall Survival With Adjuvant Letrozole Compared With Tamoxifen in the BIG 1-98 Study (2011)

Adjuvant treatment with letrozole, compared with tamoxifen, significantly reduces the risk of death, the risk of recurrent disease, and the risk of recurrence at distant sites in postmenopausal women with hormone receptor-positive breast cancer.
Homology-based control of gene expression patterns in transgenic petunia flowers (1998)

Plant transgenes may participate in two types of homology-based gene silencing. One requires transcript homology, is post-transcriptional, and is referred to as cosuppression; the other requires promoter homology, is transcriptional, and is similar to paramutation. This paper uses flower color transgenes to address the hierarchical operation of both mechanisms in plants carrying two transgene copies. It is shown that cosuppression of homologous, endogenous flower color genes by single-copy transgenes requires that the transgene be driven by a strong promoter and that the degree of cosuppression is highly sensitive to increasing transgene dosage. Together, these observations suggest that cosuppression should be a sensitive reporter of epigenetic changes in transgene transcription, such as might be caused by paramutation-like interactions between transgene loci. Intercrosses bringing together two homologous transgene loci, one a known epimutable reporter and the other a transgene inverted repeat, result in complete loss of cosuppression in some outcross progeny and a qualitative change in morphology-based patterns of cosuppression in other outcross progeny. This paramutation-like behavior suggests that the transgenes may be altered at the transcriptional level, eliminating cosuppression altogether or changing the spatial pattern of transgene transcription to produce a new pattern of cosuppression.
Figure S1 from Restraining Network Response to Targeted Cancer Therapies Improves Efficacy and Reduces Cellular Resistance (2023)

&lt;p&gt;Figure S1 shows fly drug screening assay and list of compounds screened&lt;/p&gt;
Lead and the Environment: An Approach to Educating Adults. (1999)

This study examined the effectiveness of a lead shot environmental education program directed towards adult Cree of the Mushkegowuk Territory. The outcome that was measured was a change in attitude to the lead shot issue, that is, a change of scored state from 0 (participants stating that lead shot was not a problem or they did not know whether lead shot was a problem) to 1 (participants stating that lead shot was detrimental to the environment, wildlife, and/or people of the region). Forty participants randomly selected at the beginning of the study were scored as 0. On follow-up, all people who were available
A Negative Regulatory Region in the Intracellular Domain of the Human Interferon-α Receptor (1996)

Interferon-alpha (IFN-alpha)-mediated intracellular signaling is initiated by ligand-induced receptor dimerization, tyrosine phosphorylation of the Tyk2 and Jak1 tyrosine kinases, and subsequent phosphorylation of the Stat1 and Stat2 proteins. The IFN-alpha receptor consists of at least two distinct subunits. One subunit, IFNAR1, has low affinity binding for interferon yet is required for signal transduction. We introduced mutations in the cytoplasmic domain of human IFNAR1 in order to identify residues involved in the mediation of biological responses. We took advantage of the species specificity of the interferon receptors by analyzing human IFN-alpha-induced major histocompatibility complex class I antigen expression in mouse L929 cells stably transfected with mutant human receptors. The membrane proximal 60-amino acids were insufficient to signal a biological response even though within these residues Tyk2 and Stat2 binding sites have been identified. IFN-alpha-induced receptor tyrosine phosphorylation was not critical for signaling because mutation of Tyr residues to Phe did not prevent the biological response to IFN-alpha. The deletion of a 16-amino acid region highly homologous between species created a receptor which signals an enhanced response. Tyrosine dephosphorylation is a component of this enhanced response as mutation of the Tyr residues within this region to Phe resulted in a receptor with increased sensitivity to IFN. The known signaling molecules that interact with IFNAR1 are positive regulators of IFN-alpha function. The presence of this domain in the COOH-terminal region suggests that the receptor may interact with signaling molecules that negatively regulate interferon responses.
Data from Oxidative Stress-Regulated Lentiviral TK/GCV Gene Therapy for Lung Cancer Treatment (2023)

&lt;div&gt;Abstract&lt;p&gt;Nuclear factor erythroid-2 related factor 2 (Nrf2) is a transcription factor that regulates protection against a wide variety of toxic insults to cells, including cytotoxic cancer chemotherapeutic drugs. Many lung cancer cells harbor a mutation in either Nrf2 or its inhibitor Keap1 resulting in permanent activation of Nrf2 and chemoresistance. In this study, we sought to examine whether this attribute could be exploited in cancer suicide gene therapy by using a lentiviral (LV) vector expressing herpes simplex virus thymidine kinase (HSV-TK/GCV) under the regulation of antioxidant response element (ARE), a cis-acting enhancer sequence that binds Nrf2. In human lung adenocarcinoma cells in which Nrf2 is constitutively overexpressed, ARE activity was found to be high under basal conditions. In this setting, ARE-HSV-TK was more effective than a vector in which HSV-TK expression was driven by a constitutively active promoter. In a mouse xenograft model of lung cancer, suicide gene therapy with LV-ARE-TK/GCV was effective compared with LV-PGK-TK/GCV in reducing tumor size. We conclude that ARE-regulated HSV-TK/GCV therapy offers a promising approach for suicide cancer gene therapy in cells with high constitutive ARE activity, permitting a greater degree of therapeutic targeting to those cells. &lt;i&gt;Cancer Res; 72(23); 6227–35. ©2012 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
PID vs LQ control techniques applied to an indoor micro quadrotor (2004)

The development of miniature flying robots has become a reachable dream, thanks to the new sensing and actuating technologies. Micro VTOL systems represent a useful class of flying robots because of their strong abilities for small-area monitoring and building exploration. In this paper, we present the results of two model-based control techniques applied to an autonomous four-rotor micro helicopter called quadrotor. A classical approach (PID) assumed a simplified dynamics and a modern technique (LQ) based on a more complete model. Various simulations were performed and several tests on the bench validate the control laws. Finally, we present the results of the first test in flight with the helicopter released. These developments are part of the OS4 project in our lab.
Renal excretion of galactose and galactitol in patients with classical galactosaemia, obligate heterozygous parents and healthy subjects (2003)

The age dependence of galactose and galactitol excretion was assessed in overnight-fasted galactose-1-phosphate uridyltransferase-deficient patients under dietary treatment (ages 4-34 years; n = 51), obligate heterozygous parents (ages 25-71 years; n = 49) and healthy subjects (ages 3-58 years; n = 215). Urine concentrations were analysed by stable-isotope dilution gas chromatography mass spectrometry. There was considerable interindividual variability. The intraindividual variation, however, was not age-dependent and was rather low. Excretion estimates were calculated from the creatinine-related concentrations using weight-, age- and sex-related creatinine excretion rates. Experimental evidence is presented underscoring the problems inherent in random sampling and substantiating the primary endogenous origin of galactose and galactitol in postabsorptive urine samples. Age-dependent excretion estimates were best fitted to a simple growth-related model assuming an exponential decrease with age until adulthood. According to the model, mean postabsorptive galactose and galactitol excretion in healthy subjects was similar and decreased exponentially from about 1.2 micromol/kg body weight per day in infants to about 0.2 micromol/kg body weight per day in adults. Excretion in heterozygotes was normal. In galactosaemic patients, galactose excretion was in the normal range. Galactitol excretion, however, was enhanced over 50-fold and decreased from a mean estimate of about 64 micromol/kg body weight per day in infants to about 23 micromol/kg body weight per day in adults. The results are discussed with respect to the significance of galactose and galactitol excretion for whole-body galactose removal and with respect to the applicability of urinary galactitol analysis for metabolic monitoring in galactosaemia.