gebhart/patrepeval huggingface dataset:
Protein transduction method for cerebrovascular disorders. (2009)

Many studies have shown that a motif of 11 consecutive arginines (11R) is one of the most effective protein transduction domains (PTD) for introducing proteins into the cell membrane. By conjugating this "11R", all sorts of proteins can effectively and harmlessly be transferred into any kind of cell. We therefore examined the transduction efficiency of 11R in cerebral arteries and obtained results showing that 11R fused enhanced green fluorescent protein (11R-EGFP) immediately and effectively penetrated all layers of the rat basilar artery (BA), especially the tunica media. This method provides a revolutionary approach to cerebral arteries and ours is the first study to demonstrate the successful transductionof a PTD fused protein into the cerebral arteries. In this review, we present an outline of our studies and other key studies related to cerebral vasospasm and 11R, problems to be overcome, and predictions regarding future use of the 11R protein transduction method for cerebral vasospasm (CV).
ChemInform Abstract: Facilitation of the Syntheses of Metal Perchlorate Complexes with Purines from Non‐aqueous Suspensions by Substituting Ethyl Acetate for Ethanol in the Interaction Medium. (1986)

Abstract Untersuchungen zeigen, daß die in siedendem Ethylacetat/Triethylorthoformiat durchgeführte lz2‐molare Umsetzung von hydratisierten 3d‐ Metallperchloraten [M:Cr(III), Mn(II), Fe(II), Fe(III), Co(II), Ni(II), Zn(II)] mit Purin‐Verbindungen meist zu isolierbaren lz2‐Addukten in bisher nicht erzielten, guten Ausb. führt.
Frontotemporal Dementia: Its Rediscovery (1997)

Frontotemporal dementia (FTD) is the second most frequent degenerative cause of dementia. Although described for more than a century, it remains often misdiagnosed, mistaken for Alzheimer's disease or psychiatric disorders, which is prejudicial to care and research. It is a clinical syndrome corresponding to at least three histological entities: (1) Pick's disease, (2) non-specific frontotemporal degeneration, (6 times more frequent), and (3) frontal lobe abnormalities associated with motor neurone disease. It never corresponds to Alzheimer pathology. It must be suspected in patients with behavioural dyscontrol predating memory impairment, without spatial disorientation and normal EEG.
Web Services Enabled Procurement in the Extended Enterprise: An Architectural Design and Implementation. (2003)

Earlier supply chain implementations in EDI and XML have not fully supported the real time and dynamic requirements of e-procurement, in large part due to the limitations of the technologies available. The purpose of this paper is to illustrate how Web services can be used to implement a real time and dynamic supply chain operation. In particular, we focus on an e-procurement application in the context of business-to-business (B2B) supply chain integration. A prototype system has been developed to illustrate how Web services can enhance performance of the firms engaged in B2B procurement. The architecture and design of the system presented in this paper are based on a service-oriented architecture and on a three-tier enterprise design pattern. The impacts and implications of using Web services to enable real-time and dynamic business processes are also discussed.
Development of beta-lactamase inhibitors. (1990)

The resistance of bacteria to beta-lactam antibiotics was first associated with the production of the enzyme beta-lactamase as long ago as 1940. Since then, increasing numbers of beta-lactamase-producing bacteria capable of inactivating penicillins and cephalosporins have been isolated clinically. One approach to the problem posed by beta-lactamase-producing bacteria has been to seek substances that function as inhibitors of beta-lactamase and that can be used to protect beta-lactam antibiotics from destruction by the bacterial enzymes. The first clinically available inhibitor was clavulanic acid, a metabolite of Streptomyces clavuligerus that was identified in a screening program for naturally occurring beta-lactamase inhibitors. Clavulanic acid is a potent inhibitor of many bacterial beta-lactamases and has been formulated with amoxicillin and ticarcillin to produce broad-spectrum antibiotic combinations active against beta-lactamase-producing bacteria. After the discovery of clavulanic acid, various compounds have been reported to function as inhibitors of bacterial beta-lactamases, but only sulbactam and its prodrug, sultamicillin, have become available commercially. The success of clavulanic acid has confirmed beta-lactamase inhibitors as one solution to the problem of antibiotic-resistant bacteria.
On-Node Correlation Based Data Reduction in WSN for Smart Agriculture (2020)

International audience
Cerebrospinal Fluid Biomarkers in Alzheimer’s Disease: An Invaluable Tool for Clinical Diagnosis and Trial Enrichment (2018)

Alzheimer's disease (AD) is the most common neurodegenerative disorder, affecting around 35 million people worldwide. Cerebrospinal fluid (CSF) biomarkers entered the diagnostic criteria as support for early diagnosis. The classical biochemical signature of AD includes total tau (T-tau), phosphorylated tau (P-tau), and the 42 amino acid peptide (Aβ42) of amyloid-β. Recent observations suggest that the use of CSF Aβ42:Aβ40 ratio rather than CSF Aβ42 alone could contribute to reduce inter-laboratory variation in Aβ values and increasing diagnostic performance of the CSF AD biomarkers in routine practice. However, research efforts aimed at enriching the CSF biomarker panel are ongoing. The CSF AD signature is also crucial for the design of clinical trials for AD, since it best guarantees AD pathology as the cause of cognitive impairment. Accordingly, CSF biomarkers have been now reported in the inclusion criteria of Phase I, Phase II, and Phase III clinical trials as enrichment strategy. So far, one of the most important reasons for the failure of AD clinical trials was the inclusion of participants with unlikely AD pathology. In order to implement the use of CSF biomarkers in AD routine diagnostic work-up and as accepted strategy for enriching trial populations, inter-laboratory variability should be minimized. Increasing efforts should also be devoted to promote data sharing practices, encouraging individual participant data meta-analyses.
Design of platform management provider based on HPI manageability block using CIM/WBEM standards (2005)

Traditional management solutions have not taken any management coverage on the OS-absent phase, which is composed of two subphases; out-of-service and out-of-band. This is because those management solutions use agents on the OS, which are fatefully dependent on the state of the OS. An effective out-of-band management solution enables remote "lights out" management infrastructure and logical consolidation of data centers. Using this infrastructure, we can deal remotely with most cases of dispatching engineers for high availability and serviceability and then dramatically reduce management costs. In this paper, we present the platform management provider which offers a standardized approach to enhancing manageability during the OS-absent phase as well as OS-resident phase
Noncovalent interactions between linear-dendritic copolymers and carbon nanotubes lead to liposome-like nanocapsules (2012)

Morphology (conformation) of carbon nanotubes was changed from extended- toward closed-state upon noncovalent interactions with polyglycerol-poly(ethylene glycol)-polyglycerol ABA type hydrophilic linear-dendritic copolymer and liposome-like nanocapsules were formed. Since one of the proposed reasons for the carcinogenicity of carbon nanotubes is their long lengths and rigid structures, flexible liposome-like nanocapsules prepared by this strategy could be safer and far from the asbestos-like physicochemical properties of CNTs and therefore their potential health hazards. Avoiding these health hazards, it is possible to develop CNTs for biomedical applications. Therefore biocompatibility of liposome-like nanocapsules and their ability to load and deliver anticancer drugs such as doxorubicin was evaluated and it was proved that their loading capacity is more that two grams of drug to one gram of liposome-like nanocapsules.
Hydrogel-based artificial enzyme for combating bacteria and accelerating wound healing (2020)

Artificial enzymes have provided great antimicrobial activity to combat wound infection. However, the lack of tissue repair capability compromised their treatment effect. Therefore, development of novel artificial enzyme concurrently with the excellent antibacterial activity and the property of promoting wound healing are required. Here, we demonstrated the hydrogel-based artificial enzyme composed of copper and amino acids possessed intrinsic peroxidase-like catalytic activity, which could combat wound pathogen effectively and accelerate wound healing by stimulating angiogenesis and collagen deposition. Furthermore, the system possesses good biocompatibility for practical application. The synergic effect of the hydrogel-based artificial enzyme promises the system as a new paradigm in bacteria-infected wound healing therapy.
PROTEOMIC ANALYSIS OF WILD TYPE AND LUCIFERASE REPORTER HEPG2 CELLS EXPOSED TO TCDD (2007)

peer reviewed
Decomposed Stochastic Model Predictive Control for Optimal Dispatch of Storage and Generation (2014)

This paper proposes a stochastic model predictive control (SMPC) approach to optimally dispatch energy storage and dispatchable generation in the electric energy system under uncertainties introduced by variable energy sources as well as demand. The objective is to minimize the expectation of the sum of the production and ramping costs for generators while satisfying all the system constraints. The uncertainties are represented by scenarios, resulting in a large-scale and computationally demanding optimization problem. We use the optimality condition decomposition (OCD) to decompose the SMPC problem into subproblems which can be solved in parallel thereby reducing the computation time. Both a scenario-based decomposition and a temporal-based decomposition are formulated and numerically evaluated in terms of speed and convergence using a modified IEEE 39-bus test system. Simulation results indicate that the scenario-based decomposition scheme achieves a better trade-off between convergence speed and subproblem size for the considered optimal dispatch problem.
Multipath Mitigation using GPS/INS Integrated Navigation with Adaptive Kalman Filtering (2016)

In urban canyon, the multipath errors are significant and the number of usable satellites frequently lacks. In this paper, to overcome these problems, the GPS/INS integrated navigation algorithm is used. For the absolute position measurement, the code-based GPS position is used with suitable measurement noise covariance tuning in adaptive way because the general GPS/INS integrated navigation suffers from the significant multipath error. To achieve effective mitigation of multipath error, we first analyzed the characteristics of the multipath error in urban canyon. Then based on the observation, we can effectively mitigate the multipath error in GPS/INS integrated navigation using adaptive measurement noise covariance tuning. It is important because the Kalman filter can guarantee the optimal performance when the noise covariance were properly decided. As a result, above 30% improvement of positioning accuracy was achieved.
Measuring the Stiffness of <em>Ex Vivo</em> Mouse Aortas Using Atomic Force Microscopy (2016)

Arterial stiffening is a significant risk factor and biomarker for cardiovascular disease and a hallmark of aging. Atomic force microscopy (AFM) is a versatile analytical tool for characterizing viscoelastic mechanical properties for a variety of materials ranging from hard (plastic, glass, metal, etc.) surfaces to cells on any substrate. It has been widely used to measure the stiffness of cells, but less frequently used to measure the stiffness of aortas. In this paper, we will describe the procedures for using AFM in contact mode to measure the ex vivo elastic modulus of unloaded mouse arteries. We describe our procedure for isolation of mouse aortas, and then provide detailed information for the AFM analysis. This includes step-by-step instructions for alignment of the laser beam, calibration of the spring constant and deflection sensitivity of the AFM probe, and acquisition of force curves. We also provide a detailed protocol for data analysis of the force curves.
Comparative genomics of Rhizophagus irregularis , R. cerebriforme , R. diaphanus and Gigaspora rosea highlights specific genetic features in Glomeromycotina (2019)

Glomeromycotina is a lineage of early diverging fungi that establish arbuscular mycorrhizal (AM) symbiosis with land plants. Despite their major ecological role, the genetic basis of their obligate mutualism remains largely unknown, hindering our understanding of their evolution and biology. We compared the genomes of Glomerales (Rhizophagus irregularis, Rhizophagus diaphanus, Rhizophagus cerebriforme) and Diversisporales (Gigaspora rosea) species, together with those of saprotrophic Mucoromycota, to identify gene families and processes associated with these lineages and to understand the molecular underpinning of their symbiotic lifestyle. Genomic features in Glomeromycotina appear to be very similar with a very high content in transposons and protein-coding genes, extensive duplications of protein kinase genes, and loss of genes coding for lignocellulose degradation, thiamin biosynthesis and cytosolic fatty acid synthase. Most symbiosis-related genes in R. irregularis and G. rosea are specific to Glomeromycotina. We also confirmed that the present species have a homokaryotic genome organisation. The high interspecific diversity of Glomeromycotina gene repertoires, affecting all known protein domains, as well as symbiosis-related orphan genes, may explain the known adaptation of Glomeromycotina to a wide range of environmental settings. Our findings contribute to an increasingly detailed portrait of genomic features defining the biology of AM fungi.
Three-phase/single-phase quasi-z-source matrix converter: Circuit topology, model, and analysis (2015)

A novel three-phase/single-phase quasi-Z-source matrix converter (qZS-MC) is proposed in this paper. It extends the voltage gain of traditional MC and has the inherent benefits of MC. The circuit topology is analyzed, and the model is built, which discloses the relationship between the input current harmonic components, qZS impedance and output frequency. The suitable application area is suggested on the basis of the built model and analysis result. The prototype is designed to test the proposed three-phase/single-phase qZS-MC. The identical experimental result, simulation result, and model-based result verify the proposed circuit topology and the built analytic model.
Efficient floating point precision tuning for approximate computing (2017)

This paper presents an automatic tool-chain that efficiently computes the precision of floating point variables down to the bit level of the mantissa. Our toolchain uses a distributed algorithm that can analyze thousands of variables. We successfully used the tool to transform floating point signal processing programs to their arbitrary precision fixed-point equivalent, obtaining about 82% and 66% average reduction in resources when compared to the double precision and single precision versions, respectively.
White light emission from a polymer blend light emitting diode (1996)

A new type of polymer light emitting diodes that emit white light is reported. In these diodes, several electroluminescent substituted polythiophenes have been combined to give the necessary components of the visible spectrum. These emitting polymers are then mixed with an insulating polymer to diminish the energy transfer from high-band-gap polymers to low-band-gap polymers. The resulting emission at 20 V is shown to be close to the equienergy white point as defined by the CIE (Commission Internationale de l’Eclairage).
Physicochemical Properties of Mixed Micellar Aggregates Containing CCK Peptides and Gd Complexes Designed as Tumor Specific Contrast Agents in MRI (2004)

New amphiphilic molecules containing a bioactive peptide or a claw moiety have been prepared in order to obtain mixed micelles as target-specific contrast agents in magnetic resonance imaging. The first molecule, C(18)H(37)CONH(AdOO)(2)-G-CCK8 (C18CCK8), contains a C18 hydrophobic moiety bound to the C-terminal cholecystokinin octapeptide amide (CCK 26-33 or CCK8). The second amphiphilic compound, C(18)H(37)CONHLys(DTPAGlu)CONH(2) (C18DTPAGlu) or its gadolinium complex, (C18DTPAGlu(Gd)), contains the same C18 hydrophobic moiety bound, through a lysine residue, to the DTPAGlu chelating agent. The mixed aggregates as well as the pure C18DTPAGlu aggregate, in the presence and absence of Gd, have been fully characterized by surface tension measurements, FT-PGSE-NMR, fluorescence quenching, and small-angle neutron scattering measurements. The structural characterization of the mixed aggregates C18DTPAGlu(Gd)-C18CCK8 indicates a spherical arrangement of the micelles with an external shell of approximately 21 A and an inner core of approximately 20 A. Both the DTPAGlu(Gd) complexes and the CCK8 peptides point toward the external surface. The measured values for relaxivity in saline medium at 20 MHz proton Larmor frequency and 25 degrees C are 18.7 mM(-)(1) s(-)(1). These values show a large enhancement in comparison with the isolated DTPAGlu(Gd) complex.
The 3′ Igκ enhancer contains RNA polymerase II promoters: implications for endogenous and transgenic κ gene expression (2001)

We have created a kappa transgene in which a polymerase (pol) III promoter replaces the pol II promoter. Two independent transgenic lines show somatic hypermutation of the transgene in B cells from hyperimmunized mice. Both lines transcribe transgenes from the pol III promoter in the liver. However, in spleen and spleen B cell-derived hybridomas, they also transcribe mRNA from pol II promoters located within the 3' kappa enhancer of the preceding transgene copy in a tandem transgene array. The findings demonstrate that in an array of multiple transgenes the expression (and somatic hypermutation) of an individual transgene copy must be considered in the context of the other copies. We also show that sequences around the 3' kappa enhancer in endogenous genes are transcribed. The possible role of these promoters in endogenous kappa gene expression is discussed. An unrelated finding in this study was a novel RNA splice in one hybridoma.
ChemInform Abstract: Stereochemistry. Part 78. A New Diastereoselective (3 + 2)Annulation Approach to Five‐Membered Carbocycles. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The mechanism of the thermal rearrangement of the marasmane sesquiterpene (+)-isovelleral. Cyclopropane ring closure via an intramolecular ene reaction (1990)

The reversible thermal conversion of the fungal sesquiterpene Isovelleral (2) into (1) is a unique Intramolecular ene reaction proceeding via the bicyclic enol (3), which is demonstrated by kinetic studies, deuterium incorporation, and the trapping of (3).
Sandwich Panel Cores for Blast Applications: Materials and Graded Density (2015)

Sandwich composites are of interest in marine applications due to their high strength-to-weight ratio and tailorable mechanical properties, but their resistance to air blast loading is not well understood. Full-scale 100 kg TNT equivalent air blast testing at a 15 m stand-off distance was performed on glass-fibre reinforced polymer (GFRP) sandwich panels with polyvinyl chloride (PVC); polymethacrylimid (PMI); and styrene acrylonitrile (SAN) foam cores, all possessing the same thickness and density. Further testing was performed to assess the blast resistance of a sandwich panel containing a stepwise graded density SAN foam core, increasing in density away from the blast facing side. Finally a sandwich panel containing compliant polypropylene (PP) fibres within the GFRP front face-sheet, was subjected to blast loading with the intention of preventing front face-sheet cracking during blast. Measurements of the sandwich panel responses were made using high-speed digital image correlation (DIC), and post-blast damage was assessed by sectioning the sandwich panels and mapping the damage observed. It was concluded that all cores are effective in improving blast tolerance and that the SAN core was the most blast tolerant out of the three foam polymer types, with the DIC results showing a lower deflection measured during blast, and post-blast visual inspections showing less damage suffered. By grading the density of the core it was found that through thickness crack propagation was mitigated, as well as damage in the higher density foam layers, thus resulting in a smoother back face-sheet deflection profile. By incorporating compliant PP fibres into the front face-sheet, cracking was prevented in the GFRP, despite damage being present in the core and the interfaces between the core and face-sheets.
Influence of Different Iodide Salts on the Performance of Dye-Sensitized Solar Cells Containing Phosphazene-Based Nonvolatile Electrolytes (2010)

Polyphosphazene-based electrolytes containing different iodide salts were studied as components of dye-sensitized solar cells (DSSCs). Electrolytes based on hexa[methoxyethoxyethoxycyclotriphosphazene] (MEE trimer) with dissolved LiI, NaI, NH4I, and 1-methyl-3-propylimidazolium (PMII) and I2 were examined by ac conductivity and steady-state voltammetry. These measurements gave the individual conductivities of I−, I3−, and cations in each electrolyte as a function of salt concentration. The anionic conductivities were highest in the PMII system and decreased in the order PMII > NH4I > NaI > LiI. Photovoltaic measurements of DSSCs containing these electrolytes showed the same order of performance, and electrochemical impedance spectra (EIS) under open circuit and forward bias conditions were used to study the separate impedance components of the cells. High polymeric polyphosphazene−plasticizer blends with a dissolved PMII/I2 electrolyte gave better performance in DSSCs than equivalent poly(ethylene oxide)−plasticizer electrolytes. Although the efficiencies of these DSSCs were low (1.9%), this study identified the primary loss mechanisms and suggested possible avenues for designing more efficient polyphosphazene-based cells.
Supplementary Data from Direct Targeting of <i>MYCN</i> Gene Amplification by Site-Specific DNA Alkylation in Neuroblastoma (2023)

&lt;p&gt;Supplementary Figure S1. MYCN-amplified neuroblastoma cell lines are addicted to MYCN. Supplementary Figure S2. Cytotoxic evaluation of PI polyamides targeting the MYCN gene. Supplementary Figure S3. Direct alkylation of the targeted DNA of MYCN gene. Supplementary Figure S4. MYCN-A3 and MYCN-A4 inhibit MYCN expression and cell proliferation in MYCN-amplified neuroblastoma cell lines. Supplementary Figure S5. Global impact of MYCN-A3 on gene expression in MYCN-amplified neuroblastoma cells is comparable to that of CRISPR/Cas9 targeting MYCN. Supplementary Figure S6. MYCN-A3 induces cell cycle arrest and the expression levels of p53 family genes in neuroblastoma cell lines. Supplementary Figure S7. MYCN-A3 suppresses tumor growth in human neuroblastoma xenograft mouse models. Supplementary Figure S8. Pharmacological effects of MYCN-A3 on human MYCN-amplified neuroblastoma xenograft models in vivo. Supplementary Figure S9. MYCN-A3 affects the MYCN gene loci and not non-targeted gene loci.&lt;/p&gt;