gebhart/patrepeval huggingface dataset:
Triazacyclohexane (tach) Complexes of High-Valent Rhenium: Syntheses of [(R3tach)ReO3]+ (R = −CH3, −CH2C6H5) and Its Substitution Reactions (2010)

N-Alkylated derivatives of 1,3,5-triazacyclohexane (tach) are versatile, facially coordinating ligands. We present the syntheses and full characterization of two new complexes of the [(R(3)tach)ReO(3)](+) type. In these complexes, the tach ligand is readily substituted by bi- and tridentate ligands; hence, they can be considered as sources of the [ReO(3)](+) motif for Re(VII) complexes.
Screening for MOG-IgG and 27 other anti-glial and anti-neuronal autoantibodies in ‘pattern II multiple sclerosis’ and brain biopsy findings in a MOG-IgG-positive case (2016)

This is the largest study on established anti-neural antibodies performed in MS so far. MOG-IgG may play a role in a small percentage of patients diagnosed with pattern II MS.
Measurement and repeatability of interrupter resistance in unsedated newborn infants (2009)

Interrupter resistance (R(int)) is a useful measure of airway caliber in young children, but has not been well characterized in infants-in whom there are concerns about the accurate measurement of driving pressure. This study aimed to assess the feasibility and repeatability of measuring R(int) in unsedated newborn infants, and to explore alternative algorithms for calculating driving pressure. R(int) measurement was attempted in 28 healthy term newborn infants during natural sleep using the MicroRint device. Paired R(int) measurements were achieved in 24 infants, but after screening of waveforms only 15 infants had at least 5 technically acceptable waveforms on both measurements. R(int) values obtained were comparable with reported values for airflow resistance in newborns using other methods. However, the repeatability coefficient (CR) was much higher than reported values in preschool children using standard back-extrapolation algorithms, with CR 2.47 KPa L(-1) sec (unscreened) and 2.93 KPa L(-1) sec (screened). Other algorithms gave only marginally better repeatability, with all CR values over 50% of the mean R(int) value. Using current commercially available equipment, R(int) is too poorly repeatable to be a reliable measurement of airflow resistance in newborn infants. Lower deadspace equipment is needed, but anatomical and physiological factors in the infant are also important.
Soluble expression of recombinant human CD137 ligand in Escherichia coli by co-expression of chaperones (2011)

CD137 ligand (CD137L) is a member of the tumor-necrosis factor superfamily that binds CD137 to provide positive co-stimulatory signals for T cells activation. Co-stimulation through CD137/CD137L has become one of the promising approaches for cancer therapy. Previous reports have shown that CD137L expressed in Escherichia coli resulted in inclusion bodies or low yield. In this study, the effects of five different chaperone teams on the soluble expression of recombinant human CD137L protein were explored and analyzed. The poor expression of CD137L in the cytoplasm of E. coli was improved significantly by co-expression of chaperone GroES-GroEL-Tf. After dual induction and affinity chromatography, purified recombinant CD137L was obtained at a yield of 3 mg protein per liter with purity greater than 98% from original undetectable level. Additionally, the purified recombinant CD137L could bind CD137-positive cells in a dose-dependent manner, markedly promote the growth of activated mice T cells, and elevate the release of IL-2. The present work provides an effective system for soluble expression of functional human co-stimulatory molecule CD137L, which will facilitate the clinical developments of recombinant protein drugs.
Characterization of Poly(ethylene glycol)-Modified Superoxide Dismutase:  Comparison of Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (1996)

The poly(ethylene glycol) (PEG)-modified enzyme superoxide dismutase (PEG-SOD) was characterized using capillary electrophoresis (CE), and the results were compared to those obtained using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. A free-solution CE method was developed which separated PEG-SOD species based on the number of attached PEG chains. The identity of individual CE peaks was established by isolation and subsequent molecular weight analysis by MALDI. The accuracy of the CE method in determining the average total number of PEG chains attached to the enzyme was established by comparing it with an HPLC method that quantitates the amount of PEG after hydrolytic cleavage from the modified enzyme. In addition to determining the average number of PEG chains attached to the protein, the CE method provides information on the distribution of PEG conjugation to the SOD. The total amount and distribution of coupling of PEG to the enzyme was also directly measured using MALDI. The distribution profile of PEG modification for a typical sample of PEG-SOD as determined by CE was found to be consistent with that obtained by MALDI. A quantitative comparison of the results obtained by the two techniques on PEG-SOD samples representing a range of values of PEG modification of the enzyme demonstrated good correlation, although differences were noted in some cases. Both techniques achieved a high degree of reproducibility when properly optimized. Data were also obtained on stressed samples of PEG-SOD demonstrating the ability of the CE method to detect changes in the degree and distribution of PEG conjugation for samples on stability. These two techniques provide a convenient means of characterizing the distribution of coupling of PEG to this enzyme which may have applications to other PEG modified proteins.
Mucosal transmission and pathogenesis of chronic wasting disease in ferrets (2012)

Chronic wasting disease (CWD) of cervids is almost certainly transmitted by mucosal contact with the causative prion, whether by direct (animal-to-animal) or indirect (environmental) means. Yet the sites and mechanisms of prion entry remain to be further understood. This study sought to extend this understanding by demonstrating that ferrets exposed to CWD via several mucosal routes developed infection, CWD prion protein (PrP(CWD)) amplification in lymphoid tissues, neural invasion and florid transmissible spongiform encephalopathy lesions resembling those in native cervid hosts. The ferrets developed extensive PrP(CWD) accumulation in the nervous system, retina and olfactory epithelium, with lesser deposition in tongue, muscle, salivary gland and the vomeronasal organ. PrP(CWD) accumulation in mucosal sites, including upper respiratory tract epithelium, olfactory epithelium and intestinal Peyer's patches, make the shedding of prions by infected ferrets plausible. It was also observed that regionally targeted exposure of the nasopharyngeal mucosa resulted in an increased attack rate when compared with oral exposure. The latter finding suggests that nasal exposure enhances permissiveness to CWD infection. The ferret model has further potential for investigation of portals for initiation of CWD infection.
Polarizable particles aggregation under rotating magnetic fields using scattering dichroism (2001)

We used scattering dichroism to study the combined effects of viscous and magnetic forces on the dynamics of dipolar chains induced in magnetorheological suspensions under rotating magnetic fields. We found that the chains adjust their size to rotate synchronously with the field but with a constant phase lag. Two different behaviors for the dichroism (proportional to the total number of aggregated particles) and the phase lag are found below or above a critical frequency. We obtained a linear dependence of the critical frequency with the square of the magnetization and with the inverse of the viscosity. The Mason number (ratio of viscous to magnetic forces) governs the dynamics. Therefore there is a critical Mason number below which, the dichroism remains almost constant and above which, the rotation of the field prevents the particle aggregation process from taken place being this the mechanism responsible for the decrease of dichroism. Our experimental results have been corroborated with particle dynamics simulations showing good agreement.
Antimicrobial activity of Finnish organic honeys against human pathogenic bacteria (2017)

Increasing pathogen resistance to antimicrobials threatens every human-being globally. Alternative methods and solutions for fighting against infectious diseases are urgently needed. Honey has been used as a traditional medicine for centuries and its antimicrobial properties have been revealed in several in vitro studies against wide variety of human pathogenic bacterial species, including antibiotic resistant strains. Research on antimicrobial activity of organic honeys is a novel approach and may lead to unknown antimicrobial mechanisms and factors. It also represents sustainable development. In the present study we show that Finnish organic honeys have antimicrobial activity against the growth of human pathogenic organisms E. coli, S. typhi, P. aeruginosa, K. pneumoniae, B. cereus and S. epidermidis responsible for various human diseases. Moderate concentrations of the studied organic honeys were active against most of the pathogenic organisms and thus could have applications in vivo in order to control infections caused by the studied pathogens. The determined minimum inhibitory concentrations (MIC) of the organic honeys were significant, when considering applications to medical practice.
Hit Optimization of 5-Substituted-N-(piperidin-4-ylmethyl)-1H-indazole-3-carboxamides: Potent Glycogen Synthase Kinase-3 (GSK-3) Inhibitors with in Vivo Activity in Model of Mood Disorders (2015)

Novel treatments for bipolar disorder with improved efficacy and broader spectrum of activity are urgently needed. Glycogen synthase kinase 3β (GSK-3β) has been suggested to be a key player in the pathophysiology of bipolar disorder. A series of novel GSK-3β inhibitors having the common N-[(1-alkylpiperidin-4-yl)methyl]-1H-indazole-3-carboxamide scaffold were prepared taking advantage of an X-ray cocrystal structure of compound 5 with GSK-3β. We probed different substitutions at the indazole 5-position and at the piperidine-nitrogen to obtain potent ATP-competitive GSK-3β inhibitors with good cell activity. Among the compounds assessed in the in vivo PK experiments, 14i showed, after i.p. dosing, encouraging plasma PK profile and brain exposure, as well as efficacy in a mouse model of mania. Compound 14i was selected for further in vitro/in vivo pharmacological evaluation, in order to elucidate the use of ATP-competitive GSK-3β inhibitors as new tools in the development of new treatments for mood disorders.
Fluidic circuit board with modular sensor and valves enables stand-alone, tubeless microfluidic flow control in organs-on-chips (2022)

Organs-on-chips are a unique class of microfluidic <i>in vitro</i> cell culture models, in which the <i>in vivo</i> tissue microenvironment is mimicked. Unfortunately, their widespread use is hampered by their operation complexity and incompatibility with end-user research settings. To address these issues, many commercial and non-commercial platforms have been developed for semi-automated culture of organs-on-chips. However, these organ-on-chip culture platforms each represent a closed ecosystem, with very little opportunity to interchange and integrate components from different platforms or to develop new ones. The translational organ-on-chip platform (TOP) is a multi-institutional effort to develop an open platform for automated organ-on-chip culture and integration of components from various developers. Central to TOP is the fluidic circuit board (FCB), a microfluidic plate with the form factor of a typical well plate. The FCB enables microfluidic control of multiple components like sensors or organ-on-chip devices through an interface based on openly available standards. Here, we report an FCB to integrate commercial and in-house developed components forming a stand-alone flow control system for organs-on-chips. The control system is able to achieve constant and pulsatile flow recirculation through a connected organ-on-chip device. We demonstrate that this system is able to automatically perfuse a heart-on-chip device containing co-cultures of cardiac tissues derived from human pluripotent stem cell-derived cardiomyocytes and monolayers of endothelial cells for five days. Altogether, we conclude that open technology platforms allow the integration of components from different sources to form functional and fit-for-purpose organ-on-chip systems. We anticipate that open platforms will play a central role in catalyzing and maturing further technological development of organ-on-chip culture systems.
Control, Management and Orchestration of Optical Networks: An Introduction, Challenges and Current Trends (2017)

This tutorial is an introduction to control and management; focusing on main drivers, key benefits and functional/protocol architectures. It covers multi-domain and multi-layer networks and includes complex use cases and current trends such as joint IT/network orchestration and slicing.
1.55 μm erbium doped fluoride fiber amplifiers cascade with high broadband gain flatness for multiwavelength transmission systems (1993)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text D. Ronarc'h, B. Clesca, Y. Sorel, D. Bayart, M. Guibert, L. Hamon, J. F. Kerdiles, J. L. Beylat, and M. Semenkoff, "1.55 μm erbium doped fluoride fiber amplifiers cascade with high broadband gain flatness for multiwavelength transmission systems," in Optical Amplifiers and Their Applications, Vol. 14 of 1993 OSA Technical Digest Series (Optica Publishing Group, 1993), paper PD10. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
ChemInform Abstract: DAS SOLVOLYTISCHE VERH. VON N‐BROM‐AMINEN (1972)

Abstract Bei der Umlagerung des N‐Chloramins (I) in Gegenwart von Silberionen entstehen das Umlagerungsprodukt (II), die Methoxy‐Verbindung (III) und das entsprechende freie Amin (IV), aus dem mit Hypobromit das N‐Brom‐amin (V) entsteht.
Post Oxygen Treatment Characteristics of Coke as an Anode Material for Li-Ion Batteries (2013)

The effect of a oxygen treatment on the electrochemical characteristics of a soft carbon anode material for Li-ion batteries was investigated. After a coke carbonization process at 1000 degrees C in an argon atmosphere, the samples were treated under a flow of oxygen gas to obtain a mild oxidation effect. After this oxygen treatment, the coke samples exhibited an improved initial coulombic efficiency and cycle performance as compared to the carbonized sample. High-resolution transmission electron microscopy revealed that the carbonized cokes consisted of disordered and nanosized graphene layers and the surface of the modified carbon was significantly changed after the treatment. The chemical state of the cokes was analyzed using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The enhanced electrochemical properties of the surface modified cokes could be attributed to the mild oxidation effect induced by the oxygen treatment. The mild oxidation process could have led to the elimination of surface imperfections and the reinforcement of a solid electrolyte interphase film, which resulted in the improved electrochemical characteristics.
Growth hormone treatment for childhood short stature and risk of stroke in early adulthood (2015)

Editors' Note: WriteClick this week centers on controversy over the recent article, “Growth hormone treatment for childhood short stature and risk of stroke in early adulthood.” Drs. Linglart et al. and Drs. Geffner et al. argue strongly for caution in warning patients of short stature treated with growth hormone of a possible increased stroke risk based on the study's findings, the former group pointing to previous false alarms and issues of bias, and the latter group questioning the study's data collection and analysis. Both cast doubt on the appropriateness of the study's chosen control population. Authors Coste et al. defend their methodology and assert that all available information regarding risks and benefits should be communicated to patients. —Megan Alcauskas, MD, and Robert C. Griggs, MD 

We read with interest the article by Poidvin et al.1 In 2012, an article from the Safety and Appropriateness of Growth hormone treatments in Europe (SAGhE) study group warned of cancer and mortality in growth hormone (GH)–treated patients that turned out to be flawed due to inappropriate controls of normal height.2 The largest SAGhE cohort did not detect any increase in mortality or cerebrovascular events, a report agreed and cosigned by the French team.3 Given this deplorable experience, Poidvin et al.1 …
The Relationship Between DNA-Adduct Formation and Induction of Mutations in the Hypoxanthine-Guanine Phosphoribosyl Transferase (HGPRT) Locus in Human Uroepithelial Cells (1994)

Abstract To gain an insight on the mechanisms of mutagenesis and carcinogenesis of the human bladder carcinogen 4-aminobiphenyl (ABP) in the target uroepithelia, we analyzed the cellular DNA-adducts and mutational spectra in the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) gene following exposure to N-hydroxy metabolites of ABP. [32P]-postlabelling analyses of DNA isolated from SV-40 immortalized human uroepithelial cells (HUC) exposed to N-hydroxy-4-aminobiphenyl derivatives revealed N-(deoxyguanosin-8-yl)-4-aminobiphenyl (dG-ABP) as the major adduct and N-(deoxyadenosin-8-yl)-4-aminobiphenyl (dA-ABP) as a minor adduct. The adduct profiles of the HUC-DNA obtained at different periods after carcinogen exposure showed that dG-ABP was repaired rapidly while dA-ABP persisted in HUC cultures. DNA sequence analyses of thioguanine resistant mutants of HGPRT showed base deletions (G, bp. 599; A, bp. 608; and A,T bp. 672–673). These results imply that dG-ABP and dA-ABP are repaired at different rates and that dA-ABP adduction might be primarily associated with the induction of frameshift mutations in human uroepithelia. Key Words: Human uroepithelia4-aminobiphenylBladder cancerHypoxanthine-guanine phosphoribosyl transferaseDNA-adductMutation
In-vitro fertilization of pig oocytes by frozen boar spermatozoa (1988)

In Exp. 1 pig oocytes matured in vitro were used to evaluate the fertilizability in vitro of frozen epididymal (4 boars) and ejaculated (3 boars) spermatozoa that were preincubated in modified TCM-199 for 4 h at 37 degrees C. The percentages of penetrated oocytes with the frozen epididymal spermatozoa were 0-40%. In contrast, none of the oocytes were penetrated with the frozen ejaculated spermatozoa. In Exp. 2, oocytes matured in vivo were inseminated in vitro with the frozen epididymal spermatozoa that were known to penetrate oocytes matured in vitro. The penetration rate was 79% and the percentage of polyspermic oocytes was 57%. Culture for 30 h of oocytes matured in vivo and fertilized in vitro resulted in 51% (34/67) developing to the 2-cell stage. These embryos were transferred to 2 recipient gilts. One gilt became pregnant and a litter of 3 (1 live and 2 dead) was born. These results indicate that frozen epididymal spermatozoa can be used for in-vitro fertilization in the pig.
Fluorescence correlation spectroscopy in an optical trap (2010)

Fluorescence correlation spectroscopy is one of the most sensitive methods for biomolecule and nanoparticle studies. By fluorescently labeling the target particles, statistical analysis of the fluorescence fluctuation inside the focal volume of a laser beam yield information of the concentration and diffusion of the target particles in the illuminated volume. However, the application of FCS is limited by its detection range of 10<sup>10</sup>-10<sup>14</sup> particles/mL. To overcome this sensitivity threshold on the low concentration end, we designed a hybrid system that augments FCS with optical trapping. By using the optical gradient force from a second laser focused to the same illuminated volume, we were able to show that the local concentration of particles can be enriched significantly, thus extending the useful range of FCS. In this work, we describe this novel hybrid optical method for nanoparticle detection by first considering freely diffusing particles about the illuminating volume, and then compare the results to nanoparticles under the influence of the optical trapping laser. Analysis of trapped particle number permits measurement of trapping energy as well as determine ambient concentration out of the trap. Furthermore, the hindered diffusion of trapped particles due to optical forces will be discussed.
EXPRESSION OF CONCERN: COVID‐19 re‐infection (2021)

All authors are not in conflict of interest with the content of this manuscript. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
QoS Implementation For MPLS Based Wireless Networks (2002)

Abstract: Voice has been the primary application in wireless networks to date. However, packet based applications and higher bandwidth requirements to sustain these applications are ever emerging. As in wireline packet based networks, while IP routing is well understood, IP networking by itself is still not achieved and is dependent on the backbones that can offer quality of service gurantees. Mulitprotocol Label Switching (MPLS) is a newer technology that offers service integration, layer 2 switching and connection orientedness, that allows traffic engineering control traffic flows in the network. The purpose of this paper is to show how MPLS QoS architecture can be employed to provide traffic engineering in broadband wireless networks. 1
Asymmetric Michael Addition of Chiral Lithiated Sulfonates to Nitroalkenes: Diastereo‐ and Enantioselective Synthesis of α,β‐Disubstituted γ‐Nitro and β‐Alkoxycarbonyl Methyl Sulfonates. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.