gebhart/patrepeval huggingface dataset:
Elimination of Potential Sites of Glycosylation Fails to Abrogate Complement Regulatory Function of Cell Surface CD59 (1996)

CD59 is a glycosylphosphatidylinositol-anchored membrane glycoprotein that serves as the principle cellular inhibitor of the C5b-9 membrane attack complex (MAC) of human complement. Approximately 50% of the total apparent mass of CD59 is attributable to glycosylation of a single Asn (Asn18). The deduced amino acid sequences of CD59 homologues identified in Old and New World primates as well as in rat reveal that the motif for N-linked glycosylation at the residue corresponding to Asn18 of human CD59 is invariably conserved, despite considerable sequence divergence elsewhere in the protein. Such conservation suggests that the post-translational modification at Asn18 has importance for either expression or normal function of CD59 at the cell surface. In this study, we specifically examined how deletion or transposition of the site of N-linked glycosylation in the CD59 polypeptide affects its MAC inhibitory function. Our data demonstrate that the inhibitory potency of CD59 is unaffected when glycosylation is transposed from Asn18 to another site in the polypeptide. Furthermore, we show that CD59 retains normal MAC regulatory function when mutated to eliminate all potential sites for N-linked glycosylation. These data suggest that the MAC inhibitory function of CD59 is entirely provided by residues exposed at the surface of the core polypeptide and that this core structure is not influenced by glycosylation at Asn18.
A dedicated micro-CT beamline for the Australian Synchrotron and the Remote-CT project (2013)

A dedicated micro-CT beamline is planned for the Australian Synchrotron which will extend the synchrotron's imaging and tomography capability down to the smaller scale, incorporating phase-contrast and absorption-contrast, and an additional focussing-based mode for high-resolution. The beamline will use multi-layer mirror monochromators for enhanced flux, and will focus particularly on dynamic and high throughput studies in both monochromatic and pink-beam mode. Together with the existing Imaging and Medical beamline, this beamline will produce numerous large datasets of 10 GB or more, providing a significant data-processing challenge. The Remote-CT project addresses this by combining the "MASSIVE" supercomputing GPU cluster with XLI / X-TRACT software, developed at CSIRO. This software has extensive functionality for both processing and simulation of absorption and phase-contrast tomography data and has now been modified for parallel operation on a GPU cluster to take maximum advantage of the speed-up this enables.
Ventricular global function index is associated with clinical outcomes in pediatric pulmonary hypertension (2023)

RV GFI was associated with CAO in this cohort, and inclusion in multivariable models had increased predictive value compared to RVEF. GFI uses readily available CMR data without additional post-processing and may provide additional prognostic value in pediatric PH patients beyond traditional CMR markers.
Global CO2 Recycling (1996)

Effective electrodes for electrolysis of seawater for production of hydrogen (H2) without releasing chlorine into atmosphere and catalysts for production of methane (CH4) by the reaction of carbon dioxide (CO2) with H2 have been tailored. Using these novel materials a CO2 recycling plant for substantiation of our proposal has been built on the roof of Institute for Materials Research, Tohoku University. The CO2 recycling plant consists of a desert, a coast close to the desert and an energy consuming district. At the desert electricity is generated by solar cell operation and transmitted to the coast close to the desert. At the coast H2 is produced by electrolysis of seawater using the electricity, and then CH4 is formed by the reaction of H2 and CO2. CH4 is transported to the energy consuming district. At the energy consuming district combustion of CH4 is carried out not by air but by O2 and CO2 is recovered after removing H2O from the exhaust gas composed only of CO2 and H2O. The recovered CO2 is sent again to the coast close to the desert for reproduction of CH4. The CO2 recycling plant has substantiated that the solar energy at the desert can be used by energy consumers in the form of CH4 without emitting CO2 into atmosphere.
<title>Transverse pattern modifications in a stable apertured CO2 laser resonator</title> (1990)

The effects of hard apertures on the transversal mode distribu- tion and on the output near and far field distributions are investig- ated both theoretically and experimentally. Wavefronts propagating in different directions inside a C02 laser cavity were measured at the same plane and found to have different transversal amplitude prof 1- les. The influence of intracavity aperture's location and diameter on the near field distribution and on far field beam quality were measured and calculated. Quantitative figures of merit are introduced to evaluate the departure of the transversal mode patterns from ideal gauss ians.
Guided-wave acoustooptic FM modulator (1975)

Phase modulation of guided optical waves by longitudinal sound is observed in a thin-film modulator of simple construction. The dependence of modulation sideband amplitudes upon acoustic strain is investigated, and device performance is found to be close to that predicted by theory.
Different discrete soliton states in periodic optical induced waveguide lattice (2007)

Different discrete soliton states in optical waveguide lattices are studied theoretically for the different external conditions such as the biased field, lattice spacing and the position of the input pulse. It is demonstrated that the discrete solitons can be formed in an appropriate biased field and exhibit different discrete properties in different biased field. The results also indicate that lattice spacing and the position of input pulse can affect apparently the discreteness of discrete solitons. If changing the ratio of the waist width of input beam to lattice period, the discrete solitons will be excited in more or less channels. In addition, the increase of lattice intensity almost does not affect the propagation behavior of soliton.
Synthesis and biological evaluation of novel peptidomimetics as rhodesain inhibitors (2015)

Novel rhodesain inhibitors were developed by combining an enantiomerically pure 3-bromoisoxazoline warhead with a 1,4-benzodiazepine scaffold as specific recognition moiety. All compounds were proven to inhibit rhodesain with Ki values in the low-micromolar range. Their activity towards rhodesain was found to be coupled to an in vitro antitrypanosomal activity, with IC50 values ranging from the mid-micromolar to a low-micromolar value for the most active rhodesain inhibitor (R,S,S)-3. All compounds showed a good selectivity against the target enzyme since all of them were proven to be poor inhibitors of human cathepsin L.
Chain reversals in model peptides: studies of cystine-containing cyclic peptides. 1. Conformational free energies of cyclization of hexapeptides of sequence Ac-Cys-X-Pro-Gly-Y-Cys-NHMe (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChain reversals in model peptides: studies of cystine-containing cyclic peptides. 1. Conformational free energies of cyclization of hexapeptides of sequence Ac-Cys-X-Pro-Gly-Y-Cys-NHMeP. J. Milburn, Y. Konishi, Y. C. Meinwald, and H. A. ScheragaCite this: J. Am. Chem. Soc. 1987, 109, 15, 4486–4496Publication Date (Print):July 1, 1987Publication History Published online1 May 2002Published inissue 1 July 1987https://pubs.acs.org/doi/10.1021/ja00249a010https://doi.org/10.1021/ja00249a010research-articleACS PublicationsRequest reuse permissionsArticle Views156Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
P53 Gene Mutations in Lymphoid Diseases and Their Possible Relevance to Drug Resistance (1995)

Mutations in the p53 tumor suppressor gene occur with a frequency of 12.5% in lymphoid malignancies. The viral-associated diseases, Adult T-cell Leukemia (ATL) and Burkitt's lymphoma, showed higher p53 mutation frequencies of 24% and 41%, respectively. Mutations occurred in the highly conserved regions of the p53 gene. Two new hot spots for mutation were noted in exon 7 at codons 239 and 245. The spectrum of p53 mutations differs among different cancers. Transition mutations occurring in colon and brain tumors also predominated in the majority of the lymphoid malignancies. However, B-cell chronic lymphocytic leukemia (B-CLL) and non-Hodgkin's lymphoma (NHL) had an unusually high frequency of G to T transversions. Among carcinomas of the lung, liver, breast and esophagus there is also a high frequency of G to T transversions. The differences in mutation spectra between different lymphoid diseases may be due to differences in mutagenic factors or differences in the biological properties of the p53 protein in different lymphoid compartments. Mutation of the p53 gene is associated with advanced stage of lymphoid disease and poor prognosis. For B-CLL disease, p53 mutations are associated with drug resistance. Overexpression of the bcl-2 protein is also associated with a block in apoptosis. Resistance to apoptosis could be a general mechanism for drug resistance in B-CLL and other lymphoid diseases.
Activity of Meadowfoam (Limnanthes alba) Seed Meal Glucolimnanthin Degradation Products against Soilborne Pathogens (2011)

Meadowfoam (Limnanthes alba L.) is a herbaceous winter-spring annual grown as a commercial oilseed crop. The meal remaining after oil extraction from the seed contains up to 4% of the glucosinolate glucolimnanthin. Degradation of glucolimnanthin yields toxic breakdown products, and therefore the meal may have potential in the management of soilborne pathogens. To maximize the pest-suppressive potential of meadowfoam seed meal, it would be beneficial to know the toxicity of individual glucolimnanthin degradation products against specific soilborne pathogens. Meloidogyne hapla second-stage juveniles (J2) and Pythium irregulare and Verticillium dahliae mycelial cultures were exposed to glucolimnanthin as well as its degradation products. Glucolimnanthin and its degradation product, 2-(3-methoxyphenyl)acetamide, were not toxic to any of the soilborne pathogens at concentrations up to 1.0 mg/mL. Two other degradation products, 2-(3-methoxymethyl)ethanethioamide and 3-methoxyphenylacetonitrile, were toxic to M. hapla and P. irregulare but not V. dahliae. The predominant enzyme degradation product, 3-methoxybenzyl isothiocyanate, was the most toxic compound against all of the soilborne pathogens, with M. hapla being the most sensitive with EC(50) values (0.0025 ± 0.0001 to 0.0027 ± 0.0001 mg/mL) 20-40 times lower than estimated EC(50) mortality values generated for P. irregulare and V. dahliae (0.05 and 0.1 mg/mL, respectively). The potential exists to manipulate meadowfoam seed meal to promote the production of specific degradation products. The conversion of glucolimnanthin into its corresponding isothiocyanate should optimize the biopesticidal properties of meadowfoam seed meal against M. hapla, P. irregulare, and V. dahliae.
G-protein coupling of μ-opioid receptors (OP3): elevated basal signalling activity (2000)

To determine mu-opioid receptor (OP(3)) signalling activity, guanosine 5'-[gamma-[(35)S]thio]triphosphate (GTP[(35)S]) binding to G-proteins was measured in the membranes of human embryonic kidney cells (HEK-293) transfected with mu-opioid receptor (HEK-mu). GTP[(35)S] binding to HEK-mu membranes was significantly elevated compared with HEK-293 control membranes (without OP(3)), and this was abolished by pertussis-toxin pretreatment. The irreversible antagonist beta-chlornaltrexamine (beta-CNA) dose-dependently decreased elevated basal G-protein coupling of HEK-mu to control levels in cells devoid of OP(3). This characterizes beta-CNA as an inverse OP(3) agonist. Immunoprecipitation of solubilized G-proteins with G(i3)alpha antisera demonstrated that basal GTP[(35)S] binding to G(i3)alpha was also substantially elevated in HEK-mu membranes over the control, whereas G(i3)alpha protein levels were unchanged. Basal GTP[(35)S] binding to G(i1)alpha/G(i2)alpha and G(o)alpha was also increased twofold in HEK-mu membranes over the control. Morphine further increased coupling to each of these Galpha proteins with similar potency, but not to G(q)/(11)alpha or G(s)alpha. These results indicate that the wild-type OP(3) can couple constitutively to endogenously expressed G(i3)alpha, G(i1)alpha/G(i2)alpha and G(o)alpha subunits of G-proteins in HEK-293 cells.
Diffusion Limited Photoluminescence Quantum Yields in 1-D Semiconductors: Single-Wall Carbon Nanotubes (2010)

Photoluminescence quantum yields and nonradiative decay of the excitonic S(1) state in length fractionated (6,5) single-wall carbon nanotubes (SWNTs) are studied by continuous wave and time-resolved fluorescence spectroscopy. The experimental data are modeled by diffusion limited contact quenching of excitons at stationary quenching sites including tube ends. A combined analysis of the time-resolved photoluminescence decay and the length dependence of photoluminescence quantum yields (PL QYs) from SWNTs in sodium cholate suspensions allows to determine the exciton diffusion coefficient D = 10.7 ± 0.4 cm(2)s(-1) and lifetime τ(PL) for long tubes of 20 ± 1 ps. PL quantum yields Φ(PL) are found to scale with the inverse diffusion coefficient and the square of the mean quenching site distance, here l(d) = 120 ± 25 nm. The results suggest that low PL QYs of SWNTs are due to the combination of high-diffusive exciton mobility with the presence of only a few quenching sites.
Performance of low complexity RS-BTC with M-ary Phase-Shift Keying (2004)

International audience
Ultrafast electrical spectrum analyzer based on all-optical Fourier transform and temporal magnification (2017)

Real-time electrical spectrum analysis is of great significance for applications involving radio astronomy and electronic warfare, e.g. the dynamic spectrum monitoring of outer space signal, and the instantaneous capture of frequency from other electronic systems. However, conventional electrical spectrum analyzer (ESA) has limited operation speed and observation bandwidth due to the electronic bottleneck. Therefore, a variety of photonics-assisted methods have been extensively explored due to the bandwidth advantage of the optical domain. Alternatively, we proposed and experimentally demonstrated an ultrafast ESA based on all-optical Fourier transform and temporal magnification in this paper. The radio-frequency (RF) signal under test is temporally multiplexed to the spectrum of an ultrashort pulse, thus the frequency information is converted to the time axis. Moreover, since the bandwidth of this ultrashort pulse is far beyond that of the state-of-the-art photo-detector, a temporal magnification system is applied to stretch the time axis, and capture the RF spectrum with 1-GHz resolution. The observation bandwidth of this ultrafast ESA is over 20 GHz, limited by that of the electro-optic modulator. Since all the signal processing is in the optical domain, the acquisition frame rate can be as high as 50 MHz. This ultrafast ESA scheme can be further improved with better dispersive engineering, and is promising for some ultrafast spectral information acquisition applications.
Human Reproduction and in vitro Fertilisation (1988)

Combining the basic physiological facts of human reproduction with a concise account of the techniques used for in vitro fertilization (IVF), this short text makes an important addition to material currently available for sixth form and introductory courses in higher education. Following the central theme of the Dimensions of Science Series, in which modern discoveries are placed in a social context, this book uses the topic of IVF to illustrate not only the scientific and clinical aspects of human infertility, but the way science develops and generates ethical issues of concern to all
Hepadnaviruses: Current Models of RNA Encapsidation and Reverse Transcription (1996)

Progress in the field of hepadnaviral research has advanced considerably through the use of tissue culture systems for viral expression and the development of systems for the in vitro expression of the polymerases. Although the essential similarities between hepadnaviruses and the classically studied retroviruses have been confirmed, differences between the two groups of viruses have become strikingly apparent. Synthesis of the hepadnaviral polymerase as a distinct translation product and the central role played by this protein in directing RNA encapsidation may be contrasted with the retroviral model in which polymerase is expressed as a gag-pol fusion protein and the major determinants of RNA packaging specificity reside on the gag protein. Whereas retroviruses typically carry out both proteolytic processing and RNA splicing, there is no evidence for either protein processing or splicing in hepadnaviruses. The use of a protein primer for the synthesis of minus strand DNA is without precedent among the retroviruses, which employ a host cell tRNA for this function. In both cases, strand transfer is mediated through the use of terminal sequence similarities. However, the plus and minus strand primer translocations intrinsic to hepadnaviral reverse transcription are very different from the strongstop DNA translocations that occur in retroviruses. A characteristic feature of hepadnaviral replication is transcription from the unintegrated CCC DNA and the maintenance of this DNA through a pathway of intracellular amplification, whereas retroviral expression and persistence depend on a mechanism of genomic integration.
Egg predators of an endemic Italian salamander, Salamandrina perspicillata (Savi, 1821) (2019)

We report new aquatic predators feeding on Northern spectacled salamander eggs, Salamandrina perspicillata, an endemic Italian species. Eggs were preyed upon by the leech, Trocheta bykowskii, and the trichopteran larvae of Potamophylax cingulatus and Halesus appenninus.
Luminescent organo‐polysiloxanes containing complexed lanthanide ions (2009)

Abstract In this study, a type of polysiloxane with the ester as the functional side group was prepared via a hydrosilylation reaction. The functionalized polysiloxane was then allowed to complex with Tb 3+ and Eu 3+ ions. Fourier transform infrared, ultraviolet absorption spectra and 1 H‐NMR, 13 C‐NMR and 29 Si‐NMR spectra were used to confirm the modification. Differential scanning calorimetry and thermal gravity analysis were used to study the polysiloxane's thermal properties. The complexes' luminescence spectra were recorded, and narrow‐width green and red emissions were achieved. Copyright © 2009 John Wiley &amp; Sons, Ltd.
8-OH-DPAT suppresses the induction of LTP in brain slices of the rat lateral amygdala (2003)

The effect of 8-OH-DPAT on the induction of long-term potentiation (LTP) in the lateral nucleus of the amygdala was investigated using rat horizontal brain slice preparations. Bath-applied 8-OH-DPAT decreased the field potential amplitudes in a dose-dependent manner. In the lateral amygdala synapses, 8-OH-DPAT significantly suppressed the induction of LTP evoked by a weak theta burst stimulation. This suppression of LTP was also found using a concentration of 8-OH-DPAT, which did not influence the baseline activity significantly. The specific 5-HT(1A) receptor antagonist, WAY 100,635 blocked the inhibitory effect of 8-OH-DPAT on the induction of LTP. The inhibitory effect of 5-HT(1A) receptor stimulation on amygdaloid neuronal plasticity suggests that the amygdala is a site for serotonin to exert its influence on memory of aversive events.
A wave‐front‐time dependent corona model for transmission‐line surge calculations (1999)

For transmission-line surge studies, the inclusion of corona discharge due to high voltage surges is important as well as the inclusion of frequency-dependent effects. Because the charge-voltage (q-v) curve of a lightning surge is different from that of a switching surge, a corona model should reproduce different q-v curves for different wave-front times. The present paper proposes a wave-front time dependent corona model which can express the dependence by a simple calculation procedure as accurately as a rigorous finite-difference method which requires an enormous calculation time. The simplicity enhances the incorporation of the corona model into a line model, because a large number of models are to be inserted into the line model by discretization. The q-v curves calculated by the proposed method agrees well with field tests. This paper also proposes an efficient method to deal with nonlinear corona branches in distributed-parameter line model using the trapezoidal rule of integration and the predictor-corrector method. © 1999 Scripta Technica, Electr Eng Jpn, 129(1): 29–38, 1999
Neuron and glia generating progenitors of the mammalian enteric nervous system isolated from foetal and postnatal gut cultures (2003)

Cultures of dissociated foetal and postnatal mouse gut gave rise to neurosphere-like bodies, which contained large numbers of mature neurons and glial cells. In addition to differentiated cells, neurosphere-like bodies included proliferating progenitors which, when cultured at clonal densities, gave rise to colonies containing many of the neuronal subtypes and glial cells present in the mammalian enteric nervous system. These progenitors were also capable of colonising wild-type and aganglionic gut in organ culture and had the potential to generate differentiated progeny that localised within the intrinsic ganglionic plexus. Similar progenitors were also derived from the normoganglionic small intestine of mice with colonic aganglionosis. Our findings establish the feasibility of expanding and isolating early progenitors of the enteric nervous system based on their ability to form distinct neurogenic and gliogenic structures in culture. Furthermore, these experiments provide the rationale for the development of novel approaches to the treatment of congenital megacolon (Hirschsprung's disease) based on the colonisation of the aganglionic gut with progenitors derived from normoganglionic bowel segments.