gebhart/patrepeval huggingface dataset:
Abstract 2052: MicroRNA-101 promotes apoptosis and suppresses proliferation in esophageal squamous cell carcinoma by repressing the polycomb group protein EZH2 (2010)

Abstract Background: MicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that regulate gene expression post-transcriptionally. Although aberrant expression of miRNAs has been observed in various types of human cancer, their pathophysiologic role and their relevance to tumorigenesis are still largely unknown. Overexpression of the enhancer of zeste homolog 2 (EZH2) protein, a known repressor of gene transcription, has been reported to be associated with biological malignancy in several types of cancer. Purpose: The aim of this study was (1) to analyze the expression and function of microRNA-101 (miR-101) in esophageal squamous cell carcinoma (ESCC), (2) to elucidate the regulation of the EZH2 expression by miR-101 as its target gene. Methods: miR-101 expression was investigated in 27 matched normal esophageal epitheliums and ESCCs and 12 ESCC cell lines (TE1, 4, 6, 8, 9, 10, 11, 12, 13, 14, 15, KYSE30) by TaqMan quantitative real-time-polymerase chain reaction (qRT-PCR). To evaluate the role of miR-101, cell proliferation and apoptosis was analyzed with pre-miR-101-precursor transfected cells. Next, the regulation of EZH2 by miR-101 was analyzed by real-time PCR and western blotting. In addition, to elucidate the role of EZH2 gene in ESCC, we immunohistochemically examined the expression of EZH2 protein in 120 resected ESCC specimens and determined its association with the clinicopathological characteristics and prognosis. Results: In matched 27 resected specimens, the expression of miR-101 was significantly down-regulated in ESCCs compared with normal epitheliums. All 15 ESCC cell lines also showed down-regulation of miR-101. Pre-miR-101-precursor transfected cells promote cellular apoptosis and showed significant reduction in cellular proliferation. Pre-miR-101-precursor transfected cells significantly repressed EZH2 expression in both mRNA level and protein level. In the immunohistochemical analysis of EZH2, 78 (65%) showed positive staining, while 42 (35%) lacked the staining. Positive staining for EZH2 was associated with a poor prognosis significantly. Conclusions: miR-101 promotes apoptosis and suppresses proliferation in ESCC by repressing EZH2 expression as a tumor-suppressive-microRNA. It plays an important role in progression of ESCC and may serve as a novel therapeutic target. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2052.
Growth-controlled synthesis of polymer-coated colloidal-gold nanoparticles using electrospray-based chemical reduction (2021)

In this study, the controlled nucleation and growth of gold nanoparticles (GNPs) were investigated using a self-repelled mist in a liquid chemical reaction environment. An electrospray-based chemical reduction method was conducted in the aqueous region and at room temperature to synthesize the polymeric-stabilized gold nanoparticles. The electrospray technique was used to atomize a hydrogen tetrachloraurate (III) (HAuCl4) precursor solution into electrostatically charged droplets. The atomized droplets were dispersed in an aqueous reaction bath containing L-ascorbic acid as a reducing agent and polyvinylpyrrolidone (PVP) as a stabilizer. The effect of the electrospray parameters, specifically the flow rate and electrospray droplet size, as well as the reaction conditions such as the concentration of reactants, pH, and stabilizer (PVP), were investigated. The mean diameter of the GNPs increased from around 4 to 9 nm with an increase in the electrospray flow rate, droplet size, and current passing through the electrospray jet. Spherical and monodispersed GNPs were synthesized at a relatively high flow rate of 2 mL/h and a moderate concentration of 2 mM of precursor solution. The smallest-sized GNP with a high monodispersity was obtained in the reaction bath at a high pH of 10.5 and in the presence of PVP. It is expected that continuous and mass production of the engineered GNPs and other noble metal nanoparticles could be established for scaling up nanoparticle production via the proposed electrospray-based chemical reduction method.
Abnormal trajectory detection for security infrastructure (2018)

In this work, an approach for the automatic analysis of people trajectories is presented, using a multi-camera and card reader system. Data is first extracted from surveillance cameras and card readers to create trajectories which are sequences of paths and activities. A distance model is proposed to compare sequences and calculate similarities. The popular unsupervised model One-Class Support Vector Machine (One-Class SVM) is used to train a detector. The proposed method classifies trajectories as normal or abnormal and can be used in two modes: off-line and real-time. Experiments are based on data simulation corresponding to an attack scenario proposed by a security expert. Results show that the proposed method successfully detects the abnormal sequences in the scenario with very low false alarm rate.
Dereverberation In Noisy Environments Using Reference Signals And A Maximum Likelihood Estimator (2013)

Publication in the conference proceedings of EUSIPCO, Marrakech, Morocco, 2013
Filtering Internet image search results towards keyword based category recognition (2008)

In this work we aim to capitalize on the availability of Internet image search engines to automatically create image training sets from user provided queries. This problem is particularly difficult due to the low precision of image search results. Unlike many existing dataset gathering approaches, we do not assume a category model based on a small subset of the noisy data or an ad-hoc validation set. Instead we use a nonparametric measure of strangeness [8] in the space of holistic image representations, and perform an iterative feature elimination algorithm to remove the most strange examples from the category. This is the equivalent of keeping only features that are found to be consistent with others in the class. We show that applying our method to image search data before training improves average recognition performance, and demonstrate that we obtain comparative precision and recall results to the current state of the art, all the while maintaining a significantly simpler approach. In the process we also extend the strangeness-based feature elimination algorithm to automatically select good threshold values and perform filtering of a single class when the background is given.
New tetracycline resistance determinant on R plasmids from Vibrio anguillarum (1987)

Two classes of tetracycline resistance determinants on R plasmids were detected in Vibrio anguillarum strains isolated from ayu (sweat fish; Plecoglossus altivelis) farms in Japan. Tetracycline resistance genes categorized as class B were prevalent from 1973 to 1977; however, a new tetracycline resistance gene, which was not classified into tetracycline resistance determinant class A, B, C, or D, has been prevalent since 1981.
Water Soluble Single-walled Carbon Nanotubes Using Inclusion Complex of Cyclodextrin with an Adamantane Derivative (2007)

Abstract Single-walled carbon nanotubes (SWNTs) were soluble in aqueous media by using host–guest complex between sodium adamantanecarboxylate (AdCNa) and β-cyclodextrin (β-CD), while SWNTs were insoluble with AdCNa or β-CD.
A systematic analysis of the early transcribed membrane protein family throughout the life cycle of Plasmodium yoelii (2011)

The early transcribed membrane proteins (ETRAMPs) are a family of small, highly charged transmembrane proteins unique to malaria parasites. Some members of the ETRAMP family have been localized to the parasitophorous vacuole membrane that separates the intracellular parasite from the host cell and thus presumably have a role in host-parasite interactions. Although it was previously shown that two ETRAMPs are critical for rodent malaria parasite liver-stage development, the importance of most ETRAMPs during the parasite life cycle remains unknown. Here, we comprehensively identify nine new etramps in the genome of the rodent malaria parasite Plasmodium yoelii, and elucidate their conservation in other malaria parasites. etramp expression profiles are diverse throughout the parasite life cycle as measured by RT-PCR. Epitope tagging of two ETRAMPs demonstrates protein expression in blood and liver stages, and reveals differences in both their timing of expression and their subcellular localization. Gene targeting studies of each of the nine uncharacterized etramps show that two are refractory to deletion and thus likely essential for blood-stage replication. Seven etramps are not essential for any life cycle stage. Systematic characterization of the members of the ETRAMP family reveals the diversity in importance of each family member at the interface between host and parasite throughout the developmental cycle of the malaria parasite.
Sensitive enzyme-linked immunosorbent assay for determination of malachite green and leucomalachite green in fish and fishpond water samples (2010)

An indirect competitive ELISA for the determination of malachite green and leucomalachite green in fish and fishpond water samples was developed.Leucomalachite green molecule was modified and coupled to carrier proteins to make the coating antigen and immunogen.The antibodies against LMG was obtained from rabbits.Based on the optimized conditions,IC50 value(concentration of analyte producing 50% of absorbance decrease in standard curve)and detection limit was found in the range of 0.9～2.6μg/L and 0.02～0.10μg/L,respectively.Recoveries of LMG in water and fish samples were 76.2%～95.0%.The cross –reactivity of antibody with MG was found to be 95.25%.No detectable MG and LMG and was observed in one fish sample and two fish farming water samples;while the MG and LMG concentrations in one pet fishpond water sample and another fish samle were found at 1.84μg/L and 1.38μg/L,respectively.
Changing the pill: developments toward the promise of an ultra-long-acting gastroretentive dosage form (2018)

The development of oral dosage forms providing sustained release of high potency medications over days or weeks could transform care, significantly decrease patient burden in chronic disease management and improve outcomes.
LONG SAPHENOUS VEIN HARVESTING (1986)

There is described an easy and rapid technique using the Mayo Vein Stripper to facilitate safe harvesting of the long saphenous vein for vascular reconstruction. It provides good quality vein and a superior cosmetic result without wound complications.
Transmembrane Prostatic Acid Phosphatase (TMPAP) Interacts with Snapin and Deficient Mice Develop Prostate Adenocarcinoma (2013)

The molecular mechanisms underlying prostate carcinogenesis are poorly understood. Prostatic acid phosphatase (PAP), a prostatic epithelial secretion marker, has been linked to prostate cancer since the 1930's. However, the contribution of PAP to the disease remains controversial. We have previously cloned and described two isoforms of this protein, a secretory (sPAP) and a transmembrane type-I (TMPAP). The goal in this work was to understand the physiological function of TMPAP in the prostate. We conducted histological, ultra-structural and genome-wide analyses of the prostate of our PAP-deficient mouse model (PAP(-/-)) with C57BL/6J background. The PAP(-/-) mouse prostate showed the development of slow-growing non-metastatic prostate adenocarcinoma. In order to find out the mechanism behind, we identified PAP-interacting proteins byyeast two-hybrid assays and a clear result was obtained for the interaction of PAP with snapin, a SNARE-associated protein which binds Snap25 facilitating the vesicular membrane fusion process. We confirmed this interaction by co-localization studies in TMPAP-transfected LNCaP cells (TMPAP/LNCaP cells) and in vivo FRET analyses in transient transfected LNCaP cells. The differential gene expression analyses revealed the dysregulation of the same genes known to be related to synaptic vesicular traffic. Both TMPAP and snapin were detected in isolated exosomes. Our results suggest that TMPAP is involved in endo-/exocytosis and disturbed vesicular traffic is a hallmark of prostate adenocarcinoma.
Increased vasoconstrictor sensitivity in obstructive sleep apnea (2000)

We studied vasoconstrictor sensitivity and cholinergic responsiveness of the forearm vasculature in 10 male patients with obstructive sleep apnea (OSA) and 10 healthy controls. Subjects with regular medication, known arterial hypertension, diabetes mellitus, or dyslipidemia were not included in this study. Age, body mass index, blood pressure, blood glucose, serum lipids, and baseline forearm vascular conductance (derived from venous occlusion plethysmography and intra-arterial blood pressure measurement) did not differ significantly between these two groups. With use of three dosage steps each, angiotensin II and acetylcholine were successively infused into the brachial artery. During infusion of angiotensin II, mean conductance was 39.6% lower (P = 0.002) in the OSA patients compared with that in the control subjects. Vascular responsiveness to increasing dosages of acetylcholine was not significantly altered in the OSA group. These findings suggest an enhanced vasoconstrictor sensitivity in the forearm vasculature in OSA. The hypothesis that endothelial function in OSA is impaired independently of other cardiovascular risk factors is not supported by the present results.
Direct Observation of a Photochemical Alkyne–Allene Reaction and of a Twisted and Rehybridized Intramolecular Charge-Transfer State in a Donor–Acceptor Dyad (2017)

The excited-state dynamics of an aniline-triazine electron donor-acceptor dyad with an alkyne spacer has been investigated using a combination of ultrafast broadband mid-IR and visible transient absorption and fluorescence spectroscopies. The transient IR data reveal the occurrence of an efficient alkyne to allene isomerization of the spacer with a time constant increasing from a few hundreds of femtoseconds to a few picoseconds with solvent viscosity. This process is faster than the vibrational cooling of the Franck-Condon excited state, indicative of nonequilibrium dynamics. The transient electronic absorption and fluorescence data evidence that this transformation is accompanied by a charge separation between the donor and the acceptor subunits. The allene character of the spacer implies an orthogonal orientation of the donor and acceptor moieties, similar to that proposed for twisted intramolecular charge-transfer states. Such states are often invoked in the excited-state dynamics of donor-acceptor dyads, but their involvement could never be unambiguously evidenced spectroscopically. The alkyne-allene isomerization involves not only a torsional motion but also a bending of the molecule due to the sp to sp<sup>2</sup> rehybridization of one of the alkyne carbon atoms. This twisted and rehybridized intramolecular charge transfer ("TRICT") state decays back to the planar and linear alkyne ground state on a time scale decreasing from a few hundred to ten picoseconds upon going from weakly to highly polar solvents. The different solvent dependencies reveal that the dynamics of the allene buildup are controlled by the structural changes, whereas the decay is limited by the charge recombination step.
High-Frequency Torsional Oscillation Laboratory Testing of an Entire Bottom Hole Assembly (2017)

Abstract During the downhole drilling process, severe vibration loads can occur that affect the reliability and durability of tools in bottom hole assemblies (BHA) and may cause premature damage to the tools or their subcomponents. This paper presents a laboratory test setup for high-frequency torsional oscillations (HFTO) of a 23-meter BHA. Laboratory testing is highly important for investigating this phenomenon and developing appropriate mitigation strategies. The paper provides a summary of the theoretical background for predicting the critical torsional eigenfrequencies, mode shapes, and the susceptibility of the BHA design to HFTO. In addition, the hardware and signal processing requirements for measuring HFTO and enabling differentiation of lateral and torsional vibrations are discussed. A laboratory test setup that emulates the critical mode shapes of the BHA is essential to thoroughly investigate HFTO, and to enable the development and testing of mitigation strategies. The paper presents an appropriate test setup that has been optimized in advance by finite-element simulations. Additionally, the influence of boundary conditions (i.e., clamping the BHA) is discussed. A 240-kilowatt electromagnetic shaker system is used as an excitation source, and lateral and torsional vibration are measured by multiple triaxial accelerometers along the BHA. From the measurements, torsional vibration amplitudes and operational deflection shapes are derived and compared to results from simulation models. The results from the presented laboratory HFTO test show an excellent correlation of the predicted critical frequencies and mode shapes from the simulation model to the corresponding operational deflection shapes of the tested BHA. The sensitivity of the BHA’s torsional dynamics to the boundary conditions is demonstrated by experimental variation of the string side boundary condition. Moreover, the test setup emulated the dynamic BHA’s characteristics and field-like vibration amplitudes were achieved during the test. Additionally, a method is proposed and applied to optimize correlation by variation of the excitation frequency. Using this method, a correlation of the critical HFTO mode shapes to the operational deflection shapes reproduced by the laboratory test of more than 96% can be obtained. The method demonstrates that the dynamic load profile to which the BHA is subjected during drilling operation due to HFTO can precisely be reproduced by the developed and presented laboratory setup. The ability to test entire BHAs with field-like vibration loads enables the development of fit-for-purpose drilling tools that can withstand extreme drilling conditions.
Effects of casein on flammability and mechanical characteristics of medium density fibreboards based on formaldehyde-free resin system (2023)

As one of the major milk proteins, casein has mainly been used in food and medical industries. Its applications have also been expanded to other areas, including its use as a textile fibre, coating material and as an adhesive. However, more recently, casein has shown great potential as an alternative flame retardant (FR) because it is non-toxic and biodegradable. Nevertheless, there are limited studies to investigate the effects of casein on flame retardant and mechanical properties of wood-based panels. In this research, we have set up a manufacturing process to fabricate fire resistant medium density fibreboards (MDFs). Casein is used as an FR compound to explore its effects on the fire and mechanical performance of wood-based MDF panels. Moreover, a soy flour-based resin system is selected as a replacement for the commonly used toxic formaldehyde-based resin. Two approaches are employed for the casein incorporation during the MDF manufacturing process: one is to spray casein solutions and another is to add pulverized casein particles. The spraying method with casein solution improves the flexure strength by approximately 48% compared to that of non-treated MDF samples, while the flexure strengths of MDF panels with ground casein particles dropped by nearly 26%. The addition of casein also reduced the heat release rate of the panel with a more compact char structure. The research clearly indicates the possibility of manufacturing fire-retardant casein-based MDF panels for their use in fire-prone areas.
Bloch Surface Wave Based Biosensing (2018)

An integrated biosensing platform exploiting the properties of Bloch surface waves on all-dielectric thin film stacks is described. It combines label-free sensing based on angular resonance tracking with surface wave enhanced fluorescence excitation and detection. Detailed design considerations reveal a system with defined attenuation to improve resolution limits in both modes.
Air‐Stable Polymer Electronic Devices (2007)

By introducing a titanium oxide (TiOx) layer between the active layer and the aluminum cathode in polymer-based electronic devices (see figure), devices with excellent air stability and with enhanced performance are demonstrated. The TiOx layer acts as a shielding and scavenging layer that prevents the intrusion of oxygen and humidity into the electronically active polymers, thereby improving the lifetime of unpackaged devices exposed to air by nearly two orders of magnitude.
Phase Behavior Investigation of a Live Presalt Crude Oil from Short-Wave Infrared Observation, Acoustic Wave Sensing, and Equation of State Modeling (2021)

In this work, the phase behavior of a live presalt crude oil characterized by a high CO2 content was investigated by combining measurement and modeling techniques. Because of the complexity of the fluid–fluid phase transitions observed within this system, a combination of indirect and direct detection methods was necessary to determine the phase diagram of this reservoir fluid. A shortwave infrared camera was used for direct observation of the phase transitions occurring in an oil sample placed in either a full visibility cell or a high-pressure microscopy device. In addition, an acoustic sensor working in the thickness-shear mode was used to probe phase changes during constant mass expansion experiments. The phase transitions of the live crude oil were measured from temperatures of 310 to 383 K and reported in the form of a (p, T) diagram. The obtained diagram was modeled using the Peng–Robinson equation of state with a lumping procedure that allowed representing the oil in 8 cuts including carbon dioxide. Additional measurements were carried out in a pseudobinary oil + gas model system composed of the dead oil with a synthetic gas from an equimolar content of methane and carbon dioxide to validate the experimental observations and the model predictions.
A Conserved Glycan in the C2 Domain of HIV-1 Envelope Acts as a Molecular Switch to Control X4 Utilization by Clonal Variants with Identical V3 Loops (2015)

Nearly all persons newly infected with HIV-1 harbor exclusively CCR5-using virus. CXCR4-using variants eventually arise in up to 50% of patients infected with subtypes B or D. This transition to efficient CXCR4 utilization is often co-incident with progression to AIDS. The basis for HIV-1's initial dependence on CCR5, the selective force(s) that drive CXCR4-utilization, and the evolutionary pathways by which it occurs are incompletely understood. Greater knowledge of these processes will inform interventions at all stages, from vaccination to cure. The determinants of co-receptor use map primarily, though not exclusively, to the V3 loop of gp120. In this study, we describe five clonal variants with identical V3 loops but divergent CXCR4 use. Mutagenesis revealed two residues controlling this phenotypic switch: a rare polymorphism in C1 and a highly conserved N-glycan in C2. To our knowledge, this is the first description of co-receptor usage regulated by the N-glycan at position 262.
139. The associating effect of the hydrogen atom. Part VIII. The N–H–N bond. Benziminazoles, glyoxalines, amidines, and guanidines (1941)

L. Hunter and J. A. Marriott, J. Chem. Soc., 1941, 777 DOI: 10.1039/JR9410000777