gebhart/patrepeval huggingface dataset:
Listeria monocytogenes TcyKLMN Cystine/Cysteine Transporter Facilitates Glutathione Synthesis and Virulence Gene Expression (2022)

Listeria monocytogenes is a saprophyte and a human intracellular pathogen. Upon invasion into mammalian cells, it senses multiple metabolic and environmental signals that collectively trigger its transition to the pathogenic state. One of these signals is the tripeptide glutathione, which acts as an allosteric activator of L. monocytogenes's master virulence regulator, PrfA. While glutathione synthesis by L. monocytogenes was shown to be critical for PrfA activation and virulence gene expression, it remains unclear how this tripeptide is synthesized in changing environments, especially in light of the observation that L. monocytogenes is auxotrophic to one of its precursors, cysteine. Here, we show that the ABC transporter TcyKLMN is a cystine/cysteine importer that supplies cysteine for glutathione synthesis, hence mediating the induction of the virulence genes. Further, we demonstrate that this transporter is negatively regulated by three metabolic regulators, CodY, CymR, and CysK, which sense and respond to changing concentrations of branched-chain amino acids (BCAA) and cysteine. The data indicate that under low concentrations of BCAA, TcyKLMN is upregulated, driving the production of glutathione by supplying cysteine, thereby facilitating PrfA activation. These findings provide molecular insight into the coupling of L. monocytogenes metabolism and virulence, connecting BCAA sensing to cysteine uptake and glutathione biosynthesis as a mechanism that controls virulence gene expression. This study exemplifies how bacterial pathogens sense their intracellular environment and exploit essential metabolites as effectors of virulence. <b>IMPORTANCE</b> Bacterial pathogens sense the repertoire of metabolites in the mammalian niche and use this information to shift into the pathogenic state to accomplish a successful infection. Glutathione is a virulence-activating signal that is synthesized by L. monocytogenes during infection of mammalian cells. In this study, we show that cysteine uptake via TcyKLMN drives glutathione synthesis and virulence gene expression. The data emphasize the intimate cross-regulation between metabolism and virulence in bacterial pathogens.
A novel miniature multilayer MMIC CPW single side band CPW mixer for up conversion at 44.5 GHz (2005)

This letter presents the results of a novel miniature single sideband (SSB) subharmonic direct upconverter GaAs MMIC developed in multilayer coplanar waveguide. The mixer uses 50 MHz I and Q signals to directly modulate the second harmonic of a 22.25 GHz carrier, producing the required 44.55 GHz RF output. Two pairs of antiparallel diodes reduce feedthrough of the fundamental 22.25 GHz signal to the RF output while novel coplanar waveguide/asymmetric coplanar stripline, centre conductor based structures provide matching. This 1.7/spl times/1.7 mm/sup 2/ chip also uses a previously developed, reduced size Wilkinson divider based on asymmetric coplanar stripline and a reduced size three-dimensional 90/spl deg/ coupler.
The appearance of coherence: Using cohesive properties of readers&#8217; constructed responses to predict individual differences (2021)

de propiedades cohesivas de las respuestas construidas por los lectores para
High Average Power, High Energy, Femto-second Fiber Chirped Pulse Amplification System (2007)

We have demonstrated an Yb-fiber laser system incorporating a CFBG stretcher, bandwidth optimised amplifiers and dielectric grating compressor. The system produced 135 W average power with pulse energy of 13.5 μJ. The recompressed pulse duration was 360 fs.
Studies of amino acids for inhibition of aldol condensation and dissolution of polymeric product of aldehyde in alkaline media (2013)

Compounds with acidic and basic functional groups (aspartic acid, arginine, and cystine) are evaluated for inhibition of aldol condensation and dissolution of product in alkaline media. Inhibition efficiency of amino acids were studied at varied mole ratios of acetaldehyde to antipolymerant (2, 5, and 10), reaction temperature, and caustic concentration as a function of time (0.25, 1.0, 2.0 and 24.0 h) using UV spectrophotometry. Results obtained have been compared with efficiency of known amine derivatives. Arginine showed better efficiency when compared with aspartic acid and cysteine. Furthermore, data indicate that presence of carboxylic group in addition to amine group facilitates inhibition as well as dissolution of aldol polymer in alkaline media, while compounds with amine group acts as inhibitor for aldol condensation only.
Splanchnic Oxygen Consumption and Hepatic Surface Oxygen Tensions During Isoflurane Anesthesia (1989)

CONZEN, P. F.; HOBBHAHN, J.; GOETZ, A. E.; HABAZETTL, H.; GRANETZNY, T.; PETER, K.; BRENDEL, W. Author Information
NEW GERANYLGERANYLBENZOQUINONE DERIVATIVES FROM SARGASSUM TORTILE (1979)

Abstract From the brown alga, Sargassum tortile, seven new benzoquinones and hydroquinones bearing diterpenoid side chains were isolated. 2-Geranylgeranyl-6-methylbenzoquinone, which is likely to be a basic substance of the diterpenoid-substituted benzoquinones distributed in many Sargassum species, was also obtained.
Antiplatelet Agents (2013)

Interventional radiological procedures involving anaesthesia are generally increasing.Contrastinduced nephropathy (CIN), usually defined as an increase in serum creatinine of 44 mmol litre 21 (0.5 mg dl 21 ) or a 25% increase from the baseline value 48 h after intravascular injection of contrast media, is a common and potentially serious complication of the use of iodinated contrast media in patients at risk of acute renal injury.It is an important cause of hospital-acquired renal failure, may be a difficult differential diagnosis and the incidence does not appear to have changed over the last few decades.In the general population, the incidence of CIN is estimated to be 1 -2%.However, the risk for developing CIN may be as high as 50% in some patient subgroups, such as those with diabetes mellitus and pre-existing renal impairment.The impact of CIN on clinical outcomes has been evaluated most extensively in patients undergoing percutaneous coronary intervention where it is associated with increased mortality both in hospital and at 1 yr.As treatment is limited to supportive measures while awaiting the resolution of the renal impairment, emphasis needs to be directed at prevention.
Two-dimensional transition metal carbides as supports for tuning the chemistry of catalytic nanoparticles (2018)

Supported nanoparticles are broadly employed in industrial catalytic processes, where the active sites can be tuned by metal-support interactions (MSIs). Although it is well accepted that supports can modify the chemistry of metal nanoparticles, systematic utilization of MSIs for achieving desired catalytic performance is still challenging. The developments of supports with appropriate chemical properties and identification of the resulting active sites are the main barriers. Here, we develop two-dimensional transition metal carbides (MXenes) supported platinum as efficient catalysts for light alkane dehydrogenations. Ordered Pt<sub>3</sub>Ti and surface Pt<sub>3</sub>Nb intermetallic compound nanoparticles are formed via reactive metal-support interactions on Pt/Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> and Pt/Nb<sub>2</sub>CT<sub>x</sub> catalysts, respectively. MXene supports modulate the nature of the active sites, making them highly selective toward C-H activation. Such exploitation of the MSIs makes MXenes promising platforms with versatile chemical reactivity and tunability for facile design of supported intermetallic nanoparticles over a wide range of compositions and structures.
Development of Technology for Large-Scale Economic Production of Propagules in the Rose-Scented Geranium (Pelargonium graveolens) (2014)

Experiments were carried out at Lucknow (India) to standardize protocols for derivation of cuttings from rose-scented geranium (Pelargonium graveolens) plants and converting them into rooted propagules. The protocol could give more than 1,500 cuttings/plant in ∼16 weeks. The cuttings planted in polybags or in field plots over soil + FYM or NPK developed into rooted propagules suitable for field cropping in 6 weeks. The field-produced propagules were suitable for planting in areas near their production, and the propagules produced in polybags were suitable for planting both locally and for transporting to distant locations geranium planting.
Human intention monitoring system in cyber robotics (initial report: concept and construction) (1999)

We propose the concept of a human intention monitoring system (HIMS) that can infer and understand human intentions from sensory data (e.g., environmental, system, behavioral, and biological information) input by human beings. The performance of a prototype is also discussed. HIMS will make possible the use of a high degree of human skill and creativity. It can be used in factories (e.g., in assembly lines) and field works (e.g., for telephone exchange maintenance) in tasks requiring human experience, knowledge, and skill.
Clinical and Hemodynamic Effects of Nesiritide (B‐Type Natriuretic Peptide) in Patients With Decompensated Heart Failure Receiving β Blockers (2005)

The use of beta blockers in congestive heart failure presents a therapeutic challenge for patients with acute episodes of decompensation. Such patients may be less responsive to positive inotropic agents, whereas the beneficial effects of nesiritide, which are not dependent on the beta-adrenergic receptor signal-transduction pathway, may be preserved. This analysis of the Vasodilation in the Management of Acute CHF trial evaluated the safety and efficacy of nesiritide in decompensated congestive heart failure patients receiving beta blockers. The Vasodilation in the Management of Acute CHF trial was a multicenter, randomized, controlled evaluation of nesiritide in 489 hospitalized patients with decompensated congestive heart failure. One hundred twenty-three patients were on chronic beta-blocker therapy at enrollment (31 randomized to placebo, 50 to nesiritide, and 42 to nitroglycerin). Primary end points included pulmonary capillary wedge pressure and dyspnea evaluation at 3 hours. Patients receiving nesiritide, but not IV nitroglycerin, had significantly reduced pulmonary capillary wedge pressure vs. placebo at 3 hours regardless of beta-blocker use. The use of beta blockers did not alter the beneficial effects of nesiritide on systemic blood pressure, heart rate, or dyspnea evaluation. In nesiritide-treated subjects, safety profiles were similar regardless of beta-blocker use. Thus, the clinical and hemodynamic benefits and safety of nesiritide are preserved in decompensated congestive heart failure patients receiving chronic beta blockade.
Crystal step edges can trap electrons on the surfaces of n-type organic semiconductors (2018)

Understanding relationships between microstructure and electrical transport is an important goal for the materials science of organic semiconductors. Combining high-resolution surface potential mapping by scanning Kelvin probe microscopy (SKPM) with systematic field effect transport measurements, we show that step edges can trap electrons on the surfaces of single crystal organic semiconductors. n-type organic semiconductor crystals exhibiting positive step edge surface potentials display threshold voltages that increase and carrier mobilities that decrease with increasing step density, characteristic of trapping, whereas crystals that do not have positive step edge surface potentials do not have strongly step density dependent transport. A device model and microelectrostatics calculations suggest that trapping can be intrinsic to step edges for crystals of molecules with polar substituents. The results provide a unique example of a specific microstructure-charge trapping relationship and highlight the utility of surface potential imaging in combination with transport measurements as a productive strategy for uncovering microscopic structure-property relationships in organic semiconductors.
Screen-space silhouettes for visualizing ensembles of 3D isosurfaces (2016)

Visualizing sets of isosurfaces from 3D scalar ensemble fields is a difficult task due to inherent occlusion effects, yet it is often required to analyze the uncertainty represented by such an ensemble. In this paper, we present a novel visualization technique for ensembles of isosurfaces based on screen-space silhouettes. By using silhouettes, the displayed information is reduced to avoid occlusions, yet the major shape of the surfaces can be maintained. Our approach preserves spatial coherence and does not make any assumption about the underlying surface distribution. By providing additional mechanisms, i.e., picking, clustering, cutting and animation, we enable the user to explore an ensemble of surfaces interactively.
Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa (2005)

Reversible photoswitching of individual molecules has been demonstrated for a number of mutants of the green fluorescent protein (GFP). To date, however, a limited number of switching events with slow response to light have been achieved at the single-molecule level. Here, we report reversible photoswitching characteristics observed in individual molecules of Dronpa, a mutant of a GFP-like fluorescent protein that was cloned from a coral Pectiniidae. Ensemble spectroscopy shows that intense irradiation at 488 nm changes Dronpa to a dim protonated form, but even weak irradiation at 405 nm restores it to the bright deprotonated form. Although Dronpa exists in an acid-base equilibrium, only the photoinduced protonated form shows the switching behavior. At the single-molecule level, 488- and 405-nm lights can be used to drive the molecule back and forth between the bright and dim states. Such reversible photoswitching could be repeated >100 times. The response speed to irradiation depends almost linearly on the irradiation power, with the response time being in the order of milliseconds. The perfect reversibility of the Dronpa photoswitching allows us to propose a detailed model, which quantitatively describes interconversion among the various states. The fast response of Dronpa to light holds great promise for following fast diffusion or transport of signaling molecules in live cells.
Synthesis via 2-oxazolines. IV. Asymmetric synthesis of 2-methylalkanoic acids from a chiral oxazoline (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis via 2-oxazolines. IV. Asymmetric synthesis of 2-methylalkanoic acids from a chiral oxazolineA. I. Meyers, Gerald Knaus, and K. KamataCite this: J. Am. Chem. Soc. 1974, 96, 1, 268–270Publication Date (Print):January 1, 1974Publication History Published online1 May 2002Published inissue 1 January 1974https://doi.org/10.1021/ja00808a054RIGHTS & PERMISSIONSArticle Views552Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (342 KB) Get e-Alertsclose Get e-Alerts
ChemInform Abstract: Synthesis of 1,1‐Bis(1,2,4‐triazol‐1‐yl)‐Based Potential Aromatase Inhibitors. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Use of EPR To Investigate Rotational Dynamics of Paramagnetic Contrast Agents (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of EPR To Investigate Rotational Dynamics of Paramagnetic Contrast AgentsJ. W. Chen, F. P. Auteri, D. E. Budil, R. L. Belford, and R. B. ClarksonCite this: J. Phys. Chem. 1994, 98, 51, 13452–13459Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://doi.org/10.1021/j100102a005RIGHTS & PERMISSIONSArticle Views147Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (974 KB) Get e-Alerts
Making 3D work: a classification of visual depth cues, 3D display technologies and their applications (2013)

3D display technologies improve perception and interaction with 3D scenes, and hence can make applications more effective and efficient. This is achieved by simulating depth cues used by the human visual system for 3D perception. The type of employed depth cues and the characteristics of a 3D display technology affect its usability for different applications. In this paper we review, analyze and categorize 3D display technologies and applications, with the goal of assisting application developers in selecting and exploiting the most suitable technology. Our first contribution is a classification of depth cues that incorporates their strengths and limitations. These factors have not been considered in previous contributions, but they are important considerations when selecting depth cues for an application. The second contribution is a classification of display technologies that highlights their advantages and disadvantages, as well as their requirements. We also provide examples of suitable applications for each technology. This information helps system developers to select an appropriate display technology for their applications.
Ribosomal protein L11 mutation in zebrafish leads to haematopoietic and metabolic defects (2010)

Mutations in ribosomal proteins are associated with a congenital syndrome, Diamond-Blackfan anaemia (DBA), manifested by red blood cell aplasia, developmental abnormalities and increased risk of malignancy. Recent studies suggest the involvement of p53 activation in DBA. However, which pathways are involved and how they contribute to the DBA phenotype remains unknown. Here we show that a zebrafish mutant for the rpl11 gene had defects both in the development of haematopoietic stem cells (HSCs) and maintenance of erythroid cells. The molecular signature of the mutant included upregulation of p53 target genes and global changes in metabolism. The changes in several pathways may affect haematopoiesis including upregulation of pro-apoptotic and cell cycle arrest genes, suppression of glycolysis, downregulation of biosynthesis and dysregulation of cytoskeleton. Each of these pathways has been individually implicated in haematological diseases. Inhibition of p53 partially rescued haematopoiesis in the mutant. Altogether, we propose that the unique phenotype of DBA is a sum of several abnormally regulated molecular pathways, mediated by the p53 protein family and p53-independent, which have synergistic impact on haematological and other cellular pathways affected in DBA. Our results provide new insights into the pathogenesis of DBA and point to the potential avenues for therapeutic intervention.
Genetic Analysis and Infection of SIVAGM and SIVMND (1989)

Recently, the authors determined the partial sequence of simian immunodeficiency virus (SIV) from the mandrill (SIVMND) and found SIVMND to be a new member of the HIV/SIV group, equidistant from other members, including SIVAGM. Experimentally, the African green monkey and cynomolgus monkey could be infected with SIVAGM and the cottontop tamarin with SIVMND. However, no clinical sign of an AIDS-like disease was observed in these monkeys.
Modified Cardiovascular L-type Channels in Mice Lacking the Voltage-dependent Ca2+ Channel β3 Subunit (2003)

The beta subunits of voltage-dependent calcium channels are known to modify calcium channel currents through pore-forming alpha1 subunits. Of the four beta subunits reported to date, the beta3 subunit is highly expressed in smooth muscle cells and is thought to consist of L-type calcium channels. To determine the role of the beta3 subunit in the voltage-dependent calcium channels of the cardiovascular system in situ, we performed a series of experiments in beta3-null mice. Western blot analysis indicated a significant reduction in expression of the alpha1 subunit in the plasma membrane of beta3-null mice. Dihydropyridine binding experiments also revealed a significant decrease in the calcium channel population in the aorta. Electrophysiological analyses indicated a 30% reduction in Ca2+ channel current density, a slower inactivation rate, and a decreased dihydropyridine-sensitive current in beta3-null mice. The reductions in the peak current density and inactivation rate were reproduced in vitro by co-expression of the calcium channel subunits in Chinese hamster ovary cells. Despite the reduced channel population, beta3-null mice showed normal blood pressure, whereas a significant reduction in dihydropyridine responsiveness was observed. A high salt diet significantly elevated blood pressure only in the beta3-null mice and resulted in hypertrophic changes in the aortic smooth muscle layer and cardiac enlargement. In conclusion, this study demonstrates the involvement and importance of the beta3 subunit of voltage-dependent calcium channels in the cardiovascular system and in regulating channel populations and channel properties in vascular smooth muscle cells.
Using optical nanofibres to mediate cold atom interactions (2022)

We discuss several recent advances related to optical nanofibres in cold atom systems, including two-colour dipole trap optimisation using an in-loop stochastic artificial neural network machine learner, upper bound limitations on Rydberg atom excitation due to localised ion formation, spectral lineshapes arising from the high intensity evanescent fields, and two-photon processes mediated via the evanescent field of the optical nanofibre.
A new type of silyl protecting groups in nucleoside chemistry (1978)

A new type of silyl protecting group was designed and a reagent for its introduction was obtained. This reagent, 1,3--dihalo-1,1,3,3-tetraalkyldisiloxane, allows simultaneous protection of 3′-OH and 5′-OH groups of nucleoside in high yield. The properties of the new protecting group were studied and some aspects of its applications are presented.