gebhart/patrepeval huggingface dataset:
Plant microbiome analysis after Metarhizium amendment reveals increases in abundance of plant growth-promoting organisms and maintenance of disease-suppressive soil (2020)

The microbial community in the plant rhizosphere is vital to plant productivity and disease resistance. Alterations in the composition and diversity of species within this community could be detrimental if microbes suppressing the activity of pathogens are removed. Species of the insect-pathogenic fungus, Metarhizium, commonly employed as biological control agents against crop pests, have recently been identified as plant root colonizers and provide a variety of benefits (e.g. growth promotion, drought resistance, nitrogen acquisition). However, the impact of Metarhizium amendment on the rhizosphere microbiome has yet to be elucidated. Using Illumina sequencing, we examined the community profiles (bacteria and fungi) of common bean (Phaseolus vulgaris) rhizosphere (loose soil and plant root) after amendment with M. robertsii conidia, in the presence and absence of an insect host. Although alpha diversity was not significantly affected overall, there were numerous examples of plant growth-promoting organisms that significantly increased with Metarhizium amendment (Bradyrhizobium, Flavobacterium, Chaetomium, Trichoderma). Specifically, the abundance of Bradyrhizobium, a group of nitrogen-fixing bacteria, was confirmed to be increased using a qPCR assay with genus-specific primers. In addition, the ability of the microbiome to suppress the activity of a known bean root pathogen was assessed. The development of disease symptoms after application with Fusarium solani f. sp. phaseoli was visible in the hypocotyl and upper root of plants grown in sterilized soil but was suppressed during growth in microbiome soil and soil treated with M. robertsii. Successful amendment of agricultural soils with biocontrol agents such as Metarhizium necessitates a comprehensive understanding of the effects on the diversity of the rhizosphere microbiome. Such research is fundamentally important towards sustainable agricultural practices to improve overall plant health and productivity.
N‐methyl‐D‐aspartate receptor antibody production from germinal center reactions: Therapeutic implications (2018)

Serum NR1-IgM and NR1-IgG, alongside the consistent production of NR1-IgG from circulating B cells and from ovarian teratomas suggest that ongoing germinal center reactions may account for the peripheral cell populations which secrete NR1-IgG. Cells participating in germinal center reactions might be a therapeutic target for the treatment of NMDAR-antibody encephalitis. Ann Neurol 2018;83:553-561.
Increased Zinc and Manganese in Parallel with Neurodegeneration, Synaptic Protein Changes and Activation of Akt/GSK3 Signaling in Ovine CLN6 Neuronal Ceroid Lipofuscinosis (2013)

Mutations in the CLN6 gene cause a variant late infantile form of neuronal ceroid lipofuscinosis (NCL; Batten disease). CLN6 loss leads to disease clinically characterized by vision impairment, motor and cognitive dysfunction, and seizures. Accumulating evidence suggests that alterations in metal homeostasis and cellular signaling pathways are implicated in several neurodegenerative and developmental disorders, yet little is known about their role in the NCLs. To explore the disease mechanisms of CLN6 NCL, metal concentrations and expression of proteins implicated in cellular signaling pathways were assessed in brain tissue from South Hampshire and Merino CLN6 sheep. Analyses revealed increased zinc and manganese concentrations in affected sheep brain in those regions where neuroinflammation and neurodegeneration first occur. Synaptic proteins, the metal-binding protein metallothionein, and the Akt/GSK3 and ERK/MAPK cellular signaling pathways were also altered. These results demonstrate that altered metal concentrations, synaptic protein changes, and aberrant modulation of cellular signaling pathways are characteristic features in the CLN6 ovine form of NCL.
CM 186 LC® Alloy Single Crystal Turbine Vanes (1999)

There is a need to introduce advanced turbine technology at reduced cost. SX superalloy vanes demonstrate excellent engine performance and durability benefits compared to their polycrystalline counterparts. However, their manufacturing cost can be prohibitive due to low casting and solution heat treatment yields due to rejectable grain defects. High purity (carbon and boron free), ultra high creep and fatigue strength SX alloys are limited to low angle boundaries (LABs) normally not exceeding 6° in critical airfoil locations. Carbon (C) and boron (B) containing SX superalloys (Ross, et al., 1996) can accommodate low angle boundaries in the 9°–12° range with an overall sacrifice in creep and fatigue properties. Aero engine vane segments with complex configurations, can result in not only LAB defects exceeding 9°–12° but also high angle grain boundary (HAB) defects ≥ 15° occurring during the SX solidification process. This is further excaberated by recrystallised grains occurring during solution heat treatment from residual casting stresses and associated strain. CM 186 LC® is a hafnium (Hf) containing nickel-base superalloy developed for directionally solidified (DS) columnar grain turbine airfoils. SX casting experience — development and production — has shown the alloy can be readily cast into aero turbine multi-airfoil segments. Mechanical property and turbine engine testing show the alloy can accommodate grain boundaries at least up to 30° resulting in high SX casting yields. The SX vane components are either used as-cast or approximately 50% partial solutioned which avoid any recrystallisation (Rx) problems. Component costs can be &lt; 50% of that of a conventional high purity SX alloy. Mechanical property, oxidation and coating performance characterisation studies on SX CM 186 LC (including DS test pieces) and turbine engine test and application experience show a 72°F (40°C) metal temperature capability improvement (thin wall) over DS MAR M 002 alloy.
Array Architecture for a Nonvolatile 3-Dimensional Cross-Point Resistance-Change Memory (2011)

This work explores the design and capabilities of a three-dimensional cross-point array structure suitable for use with resistance-change non-volatile memory. The resistance-change cell serves as both the access element and the memory element, eliminating the need for individual selection devices. This work presents novel architecture and circuit techniques that minimize leakage current effects while maintaining a high effective bit density. A test chip fabricated in 0.18 μm CMOS technology verifies the architecture and circuit functionality. The performance of an 8 Gb memory chip built in 65 nm technology has been simulated. A random access time of 104 ns is achieved with a power dissipation of 61.2 mW. This makes the 3D cross-point memory competitive with NOR flash in terms of read time, and competitive with NAND flash in terms of area efficiency.
PP.23.18 (2015)

Objective: Recent studies have shown that hyperaldosteronism in patients with primary aldosteronism (PA) is associated with vascular fibrosis which may be reflected in increased pulse wave velocity (PWV) but there is no studies that assessed the local stiffness of the carotid arteries in PA patients. To evaluate the changes of the renin-angiotensin-aldosterone system (RAAS), dynamics of blood pressure (BP) as well as stiffness of aorta and carotid arteries in patients with established diagnosis of adrenal aldosteroma before surgery and after 6 months of follow-up.Design and method: We studied 8 patients before and 6 months after adrenalectomy for aldosterone producing adenoma (APA). The following parameters were assessed: clinic BP, plasma renin activity (PRA) and aldosterone concentration (PAC) («IMMUNOTEC», FRANCE); carotid-femoral pulse wave velocity (cfPWV) (Sphygmocor, AtCor, Australia); Distensibility Coefficient (DC), Cross-sectional compliance (CC) and Stiffness index ß (IU-22, Philips, Holland). Data were represented as median (Med) and 25%-75% interquartile range or mean and standard deviation. p-value < 0,05 was considered as statistically significant. Results: As compared to baseline normalization of PAC, PRA and serum potassium levels were observed after adrenalectomy in all cases. All changes were statistically significant. Clinic BP also statistically significant decreased. Target levels of blood pressure were achieved in 5 patients. Moreover adrenalectomy also resulted in improvment of elastic properties: CC and DC increased significant and Stiffness index ß and PWV decreased also statistically significant. The main results are shown in Table 1. Conclusions: Our results indicate that adrenalectomy in patients with APA has a beneficial effect not only on BP and RAAS parameters but also on indices of systemic arterial compliance and local carotid stiffness. It is the first time we demonstrate the associate of effective adrenalectomy in PA with positive dynamics in parameters of local carotid stiffness.
Fictibacillus aquaticus sp. nov., isolated from downstream river water (2017)

A Gram-stain-positive, facultatively anaerobic bacterial strain, GDSW-R2A3<sup>T</sup>, was isolated from a downstream water sample collected from the river Ganges, India. Analysis of the 16S rRNA gene sequence of strain GDSW-R2A3<sup>T</sup> revealed its affiliation to the family Bacillaceae. Further analysis using a polyphasic approach revealed that strain GDSW-R2A3<sup>T</sup> was most closely related to the genus Fictibacillus. Analysis of the almost-complete (1488 bp) 16S rRNA gene sequence of strain GDSW-R2A3<sup>T</sup> revealed the highest level of sequence similarity with Fictibacillus phosphorivorans CCM 8426<sup>T</sup> (98.3 %) and Fictibacillus nanhaiensis KCTC 13712<sup>T</sup> (98.3 %) followed by Fictibacillus barbaricus DSM 14730<sup>T</sup> (98.0 %). The digital DNA-DNA hybridization and average nucleotide identity (ANI) values between strain GDSW-R2A3<sup>T</sup> and the most closely related taxon, F. phosphorivorans CCM 8426<sup>T</sup>, were 20.3 and 78.2 %, respectively. The DNA G+C content of the strain was 44.2 mol%. The cell-wall amino acid was meso-diaminopimelic acid. Polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three aminophospholipids, two phospholipids and one unidentified lipid; the major menaquinone was MK-7; iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0 were the major fatty acids. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it can be concluded that strain GDSW-R2A3<sup>T</sup> represents a novel species of the genus Fictibacillus, for which the name Fictibacillus aquaticus sp. nov. is proposed. The type strain is GDSW-R2A3<sup>T</sup> (=VTCC-B-910015<sup>T</sup>=CCM 8782<sup>T</sup>).
Psychometric properties of the brief version of the questionnaire of olfactory disorders in patients with chronic rhinosinusitis (2021)

The 7-item B-QOD captures a structurally coherent and reliable single dimension, with minimal information loss and excellent external predictive utility when compared to the QOD-NS.
Sorivudine (1995)

Sorivudine provides a unique nucleoside analog with significantly enhanced both in vitro and in vitro activity and enhanced oral bioavailability. Early indications from controlled studies indicate that sorivudine therapy is superior to acyclovir for the treatment of localized zoster in individuals with HIV infection and adults with chicken pox. However, these studies await peer evaluation. Importantly, recent experience in Japan indicates administration of sorivudine with 5-fluorouracil (5-FU) is contraindicated. Sorivudine inhibits dihydropyrimidine dehydrogenase, which is required for the metabolism of 5-FU. As a consequence, toxic levels of 5-FU accumulate in the plasma and have led to the deaths of nearly 30 patients in Japan. One might question, as these data unfold, the relative value of drugs with such enhanced in vitro activity and oral bioavailability as compared with standard therapeutic agents. Should accelerated healing occur, but not as dramatically as would have been anticipated from the in vitro data, unique approaches to the management of herpes zoster will need to be developed if further improvement is desired. Regardless, sorivudine appears superior to acyclovir for acceleration of cutaneous healing and, importantly, can be administered once daily in significantly smaller concentrations. These findings in and of themselves should allow for licensure of the compound in other developed societies.
Expression of CD68 in Non‐Myeloid Cell Types (2008)

CD68, the human homologue of macrosialin, is commonly regarded as a selective marker for human monocytes and macrophages. Its expression is thought to be regulated by a macrophage-specific promoter. However, several immunohistochemical studies have indicated that CD68 antibodies also react with other haematopoietic and non-haematopoietic cell types. We investigated the expression of CD68 in various primary cells and carcinoma cell lines using immunohistochemistry, flow cytometry, Western blot analysis and qRT-PCR. Weak but significant immunoreactivity was detected in lymphocytes and several tumour cell lines whereas staining of primary fibroblasts and endothelial cells was comparable to macrophages. The intensity of CD68 staining in individual cell types depended on the antibody clone and the fixation technique. Anti-CD68 mAb KP1 should be used with great caution for frozen tissue sections due to its reactivity with a wide variety of cell types. Also, care should be taken when distinguishing macrophages from fibroblasts/stromal cells in paraffin sections after formalin fixation since both cell types are stained highly positive for CD68. In accordance, mRNA expression of CD68 was not only detected in macrophages and monocytes but also in fibroblasts as well as endothelial cells and tumour cells, although with a varying intensity. Cloning of full length 5'-sequences and determination of transcription start sites shows that macrophages and fibroblasts initiate transcription within the known promoter region; however, from different start sites, indicating alternative promoter architecture in myeloid versus non-myeloid cells. We suggest that CD68 is not a selective macrophage marker but rather a lysosomal protein that is enriched in macrophages.
Application of air-lock method in the deltoid intramuscular injection of COVID-19 vaccine (2022)

Objective To investigate the clinical application value of air-lock method in the deltoid intramuscular injection of COVID-19 vaccine. Methods From January 27, 2021 to February 1, 2021, 780 recipients of vaccine were selected as subjects, and they were divided into a control group and a test group, with 390 cases each using a digital sampling method. The control group adopted the conventional injection method, and the test group adopted the air-lock injection method. The rate of blood extravasation or injection solution extravasation and pressing time of the two groups after injection were compared. Results The rate of blood extravasation and injection solution extravasation in the observation group were lower than those in the control group(P＜0. 01). The pressing time after the injection in the observation group was shorter than that of the control group(P＜0. 01). Conclusion The air-lock method in the deltoid intramuscular injection of COVID-19 vaccine can effectively reduce the incidence of bleeding at the injection site and the extravasation of the injection solution, ensure the vaccine injection dose, shorten the pressing time, and reduce the environmental pollution caused by the drug solution extravasation. It is an simple and efficient injection method, which is worthy of promotion and application. (目的 探讨空气闭锁(Air-lock)肌肉注射法在新型冠状病毒灭活疫苗注射中的临床实践与应用价值。方法 2021年1月27日—2月1日, 选取780例接种者作为受试对象, 随机分为对照组(n=390)和研究组(n=390)。对照组采用常规排气注射法, 研究组采用Air-lock注射法。比较两组注射后发生血液和药液渗出情况及按压时间。结果 研究组血液渗出率和药液渗出率均低于对照组, 差异有统计学意义(P＜0. 01)。研究组注射完毕后按压时间短于对照组, 差异有统计学意义(P＜0. 01)。结论 在注射新型冠状病毒灭活疫苗中实施Air-lock注射法, 可以有效的降低注射位置出血及药液外渗, 保证新型冠状病毒灭活疫苗注射剂量、缩短按压时间, 减少药液外渗所致环境污染, 同时操作简单、接种高效的特点, 具有推广应用价值。)
Functional Effects of EPS-Producing Bifidobacterium Administration on Energy Metabolic Alterations of Diet-Induced Obese Mice (2019)

Obesity has been recognized by the World Health Organization as a global epidemic. The gut microbiota is considered as a factor involved in the regulation of numerous metabolic pathways by impacting several functions of the host. It has been suggested that probiotics can modulate host gene expression and metabolism, and thereby positively influence host adipose tissue development and obesity related-metabolic disorders. The aim of the present work was to evaluate the effect of an exopolysaccharide (EPS)-producing <i>Bifidobacterium</i> strain on host glucose and lipid metabolism and the gut microbial composition in a short-term diet-induced obesity (DIO) in mice. C57BL/6J male mice were randomly divided into three groups: a control group that received control standard diet, a group fed a high-fat diet (HF), and a group fed HF supplemented with <i>Bifidobacterium animalis</i> IPLA R1. Fasting serum insulin as well as triglycerides accumulation in the liver were significantly reduced in the group receiving <i>B. animalis</i> IPLA R1. The treatment with the EPS-producing <i>B. animalis</i> IPLA R1 tended to down-regulate the expression of host genes involved in the hepatic synthesis of fatty acids which was concomitant with an upregulation in the expression of genes related with fatty acid oxidation. <i>B. animalis</i> IPLA R1 not only promoted the increase of <i>Bifidobacterium</i> but also the levels of <i>Bacteroides-Prevotella</i>. Our data indicate that the EPS-producing <i>Bifidobacterium</i> IPLA R1 strain may have beneficial effects in metabolic disorders associated with obesity, by modulating the gut microbiota composition and promoting changes in lipids metabolism and glucose homeostasis.
Fine Doppler frequency estimation in GNSS signal acquisition process (2012)

It is known that signal acquisition can be treated as a two-dimensional search and all the possible results are located in the search plane as different cells. In this paper, the theoretical expression of the cross-ambiguity function (CAF) is analyzed, and the property of the approximate expression is exploited, while it is seldom addressed in previous GNSS receiver architectures. Based on the property of the approximate CAF expression, a novel approach is proposed. In this approach, the information in the region of main peak is used to refine the Doppler frequency in the form of a simple interpolation equation. According to the results, in the absence of noise, the frequency estimation error could be theoretically decreased to zero. In the presence of noise, the novel method is equivalent to the least-square solution in terms of accuracy, but it has a significantly lower implementation complexity. Last, an averaging method is proposed to reduce the influence of noise. It shows that averaging operation can decrease the error to (-10Hz, 10Hz) in 90% of the cases when signal-to-noise ratio is C/NO=43dBHz.
Formulation of manufacturing strategies using organizational knowledge created via e-business mechanisms (2010)

Eff ective manufacturing strategies have been developed and explored in recent years. Successful manufacturing strategies can result in high manufacturing performance and enable manufacturers to obtain powerful competitive advantages. The question of how to formulate a manufacturing strategy that provides these benefi ts is an important one. This study explores the importance of organizational knowledge creation to the formulation of manufacturing strategy and enhancement of performance and investigates whether organizational knowledge can be created via an e-business mechanism. In this study, we collected 521 observations from a global sample drawn from the International Manufacturing Strategy Survey (IMSS) database and tested the data using structural equation modeling (SEM).
Translation reprogramming by eIF3 linked to glioblastoma resistance (2020)

Intrinsic resistance to current therapies, leading to dismal clinical outcomes, is a hallmark of glioblastoma multiforme (GBM), the most common and aggressive brain tumor. Understanding the underlying mechanisms of such malignancy is, therefore, an urgent medical need. Deregulation of the protein translation machinery has been shown to contribute to cancer initiation and progression, in part by driving selective translational control of specific mRNA transcripts involved in distinct cancer cell behaviors. Here, we focus on eIF3, a multimeric complex with a known role in the initiation of translation and that is frequently deregulated in cancer. Our results show that the deregulated expression of eIF3e, the e subunit of eIF3, in specific GBM regions could impinge on selective protein synthesis impacting the GBM outcome. In particular, eIF3e restricts the expression of proteins involved in the response to cellular stress and increases the expression of key functional regulators of cell stemness. Such a translation program can therefore serve as a double-edged sword promoting GBM tumor growth and resistance to radiation.
Universal Hall mobility inc-axis-orientedY0.5Ca0.5Ba2Cu3O7−δthin films (1996)

Transport properties of $c$-axis-oriented ${\mathrm{Y}}_{1\ensuremath{-}x}{\mathrm{Ca}}_{x}{\mathrm{Ba}}_{2}{\mathrm{Cu}}_{3}{\mathrm{O}}_{7\ensuremath{-}\ensuremath{\delta}}$ thin films with a wide range of $x=0\ensuremath{-}0.5$ and $\ensuremath{\delta}=0.08\ensuremath{-}1.0$ have been systematically investigated. Hall mobility ${\ensuremath{\mu}}_{H}$ in all the films satisfies the relation of ${\ensuremath{\mu}}_{H}^{\ensuremath{-}1}=\ensuremath{\alpha}{T}^{2}+C$ ($\ensuremath{\alpha}$ and $C$ are constants). By changing $x$ and $\ensuremath{\delta}$, we have successfully excluded the contribution of Cu-O chains in ${\ensuremath{\mu}}_{H}^{\ensuremath{-}1}$, and shown that $\ensuremath{\alpha}$ is essentially independent of doping level and disorder. Moreover, the doping-independent $\ensuremath{\alpha}$ is universal in various high-${T}_{c}$ superconducting cuprates, and this indicates that ${\ensuremath{\mu}}_{H}^{\ensuremath{-}1}$ is more fundamental than ${R}_{\mathrm{ab}}^{H}$.
The threads that tie protein-folding diseases (2014)

From unicellular organisms to humans, cells have evolved elegant systems to facilitate careful folding of proteins and the maintenance of protein homeostasis. Key modulators of protein homeostasis include a large, conserved family of proteins known as molecular chaperones, which augment the folding of nascent polypeptides and temper adverse consequences of cellular stress. However, errors in protein folding can still occur, resulting in the accumulation of misfolded proteins that strain cellular quality-control systems. In some cases, misfolded proteins can be targeted for degradation by the proteasome or via autophagy. Nevertheless, protein misfolding is a feature of many complex, genetically and clinically pleiotropic diseases, including neurodegenerative disorders and cancer. In recent years, substantial progress has been made in unraveling the complexity of protein folding using model systems, and we are now closer to being able to diagnose and treat the growing number of protein-folding diseases. To showcase some of these important recent advances, and also to inspire discussion on approaches to tackle unanswered questions, Disease Models & Mechanisms (DMM) presents a special collection of reviews from researchers at the cutting-edge of the field.
From Ideomotor Theory to the IAT in Just 35 Years (2022)

The title’s 35 years are from June 1963, when I received my PhD, to June 1998, when the article that introduced the Implicit Association Test was published (Greenwald et al., 1998). This chapter will be replete with digressions from the path between ideomotor theory and the IAT. I’ve italicized the digressions to make them obvious.