gebhart/patrepeval huggingface dataset:
Glycosylation of Low-Density Lipoprotein Enhances Cholesteryl Ester Synthesis in Human Monocyte-Derived Macrophages (1988)

Glucose can react with the lysine residues of low-density lipoproteins (LDLs) and convert the lipoprotein to a form with a receptor-mediated uptake by cultured cells that is impaired. However, in contrast to other modified lipoproteins taken up by both murine and human macrophages via the scavenger-receptor pathway that may induce the formation of foam cells, glycosylated LDL is not recognized by murine macrophages, and thus far, it has not been shown to lead to marked intracellular accumulation of cholesterol in human macrophages. This study illustrates that glycosylated LDL incubated with human monocyte-derived macrophages, at a concentration of 100 micrograms LDL/ml medium, stimulates significantly more cholesteryl ester (CE) synthesis than does control LDL (10.65 +/- 1.5 vs. 4.8 +/- 0.13 nmol.mg-1 cell protein.20 h-1; P less than .05). At LDL concentrations similar to those of plasma, the rate of CE synthesis in macrophages incubated with glycosylated LDL is more markedly enhanced than that observed in cells incubated with control LDL (3-fold increase). The marked stimulation of CE synthesis in human macrophages exposed to glycosylated LDL is paralleled by a significant increase in CE accumulation in these cells (P less than .001). The increase in CE synthesis and accumulation seem to be mediated by an increase in the degradation of glycosylated LDL by human macrophages. Glycosylated LDL enters the macrophages and is degraded by the classic LDL-receptor pathway in slightly smaller amounts than control LDL, but its degradation by pathways other than the classic LDL receptor or scavenger receptor is markedly enhanced.(ABSTRACT TRUNCATED AT 250 WORDS)
A new synthetic route to 3,4-dihydro-2H-1,4-thiazines (1974)

3,4-Dihydro-2H-1,4-thiazine-6-carboxylates have been synthesised by reaction of 2-mercaptoethylamine with 2,3-dibromoacrylic esters. Of particular value for the preparation of analogues of the cephalosporins is the direct conversion of 2-mercaptoethylamine into 3,4-dihydro-2H-1,4-thiazine-5-carboxylic acids containing 6-carboxylate groups.
Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate (2010)

Our results indicate that despite a well conserved core of genes there is significant genome variation between Giardia isolates, both in terms of gene content, gene polymorphisms, structural chromosomal variations and surface molecule repertoires. This study improves the annotation of the Giardia genomes and enables the identification of functionally important variation.
Thermosetting Cellular Elastomers Reinforced with Carbon Black and Silica Nanoparticles (2001)

Closed-cell materials were prepared from two types of thermo-setting elastomers: carboxyacrylonitrile-butadiene copolymer (nitrile) and iso-butylene-isoprene copolymer (butyl) filled with nanometer-size particles of carbon black and silica for the purpose of reinforcing. A chemical-blowing agent, p, p′-oxybis(benzene-sulfonyl hydrazide), which decomposes rapidly between 153° and 160°C with a release of nitrogen, and a low-pressure compression molding process were used in the fabrication. The resulting cellular materials had a density range of 0.7 to 0.8 g/cc. A balance between the cure and the blow rates is needed to provide satisfactory processing. The curing characteristics of the formulations were studied with an oscillating disk rheometer. Viscoelastic properties were gathered with a dynamic mechanical analyzer over a -90° to 80°C temperature interval. Glass transition temperatures were determined with a differential scanning calorimeter. Other properties, such as thermal stability, abrasion resistance, tensile strength, tear strength, and hardness, were also determined. The data indicate that the cellular nitrile materials should excel in applications where wear-durability is needed, with nitrile materials containing carbon black being more abrasion resistant than those reinforced with silica; butyl cellular materials, on the other hand, either with carbon black or silica, should excel in applications where good flexibility and energy dissipation at temperatures below -40°C are desired.
A Ka-band Interferometer for Cryospheric Applications-Instrument Description and First Results (2008)

Characterization of the Earth's oceanic and cryospheric topography is among NASA's strategic goals for the next decade. An effective technique for achieving such measurements is radar interferometry. For this technology, use of mm-wave frequencies (Ku- and Ka-band) is appealing due to the proportional size of the space borne structure to the observing wavelength and minimal snow penetration. At such high frequencies, achieving stability in instrument performance and the mechanical deployment structure for accurate measurements is an engineering challenge. To address this, the Microwave Remote Sensing Laboratory at the University of Massachusetts is developing a high performance Ku- and Ka-band interferometer as a prototype for a space borne instrument. The interferometer will be used in a scaled-down ground-based configuration. This paper presents an instrument description of the Ka-band interferometer (the down-converter in particular) and first results from its performance evaluation.
Compact multiple-valued multiplexers using negative differential resistance devices (1996)

Quantum electronic devices with negative differential resistance (NDR) characteristics have been used to design compact multiplexers. These multiplexers may be used either as analog multiplexers where the signal on a single select line selects one out of four analog inputs, or as four-valued logic multiplexers where the select line and the input lines represent one of four quantized signal values and the output line corresponds to the selected input. Any four-valued logic function can be implemented using only four-valued multiplexers (also known as T-gates), and this T-gate uses just 13 devices (transistors) as compared to 44 devices in CMOS. The design of the T-gate was done using a combination of resonant tunneling diodes (RTD's) and heterojunction bipolar transistors (HBT's) with the folded I-V characteristic (NDR characteristic) of the RTD's providing the compact logic implementation and the HBT's providing the gain and isolation. The application of the same design principles to the design of T-gates using other NDR devices such as resonant tunneling hot electron transistors (RHET's) and resonant tunneling bipolar transistors (RTBT's) is also demonstrated.
Effects of the taxanes paclitaxel and docetaxel on edema formation and interstitial fluid pressure (2004)

Interstitial fluid pressure (P(if)) is important for maintaining constant interstitial fluid volume. In several acute inflammatory reactions, a dramatic lowering of P(if) has been observed, increasing transcapillary filtration pressure and favoring initial and rapid edema formation. This lowering of P(if) seems to involve dynamic beta(1)-integrin-mediated interactions between connective tissue cells and extracellular matrix (ECM) fibers. beta(1)-Integrins are adhesion receptors responsible for the attachment of connective tissue cells to the ECM providing a force-transmitting physical link between the ECM and cytoskeleton. Disruption of actin filaments leads to lowering of P(if) and edema formation, suggesting a role for actin filaments. The aim of this study was to further investigate the role of the cytoskeleton in the control of P(if) by studying the effect of microtubuli fixation using paclitaxel and docetaxel. P(if) was measured with the micropuncture technique. Albumin extravasation (E(alb)) was measured using (125)I-labeled albumin. Paclitaxel and docetaxel were tested locally on foot skin in female Wistar rats. Paclitaxel (6 mg/ml) reduced P(if) from -1.5 +/- 1.0 mmHg in controls to -4.9 +/- 2.6 mmHg after 30 min (P < 0.05) in a dose-dependent manner (P < 0.05). Docetaxel caused a similar lowering of P(if). Both paclitaxel and docetaxel increased E(alb) compared with Cremophor EL and saline control (P < 0.05). Pretreatment with phalloidin before paclitaxel, causing fixation of actin filaments, abolished the lowering of P(if) caused by paclitaxel. This study confirms several previous studies demonstrating that connective tissue cells influence P(if) and edema formation.
In silico Docking Analysis for Blocking JUNO‐IZUMO1 Interaction Identifies Two Small Molecules that Block in vitro Fertilization (2022)

Combined hormone drugs are the basis for orally administered contraception. However, they are associated with severe side effects that are even more impactful for women in developing countries, where resources are limited. The risk of side effects may be reduced by non-hormonal small molecules which specifically target proteins involved in fertilization. In this study, we present a virtual docking experiment directed to discover molecules that target the crucial fertilization interactions of JUNO (oocyte) and IZUMO1 (sperm). We docked 913,000 molecules to two crystal structures of JUNO and ranked them on the basis of energy-related criteria. Of the 32 tested candidates, two molecules (i.e., Z786028994 and Z1290281203) demonstrated fertilization inhibitory effect in both an <i>in vitro</i> fertilization (IVF) assay in mice and an <i>in vitro</i> penetration of human sperm into hamster oocytes. Despite this clear effect on fertilization, these two molecules did not show JUNO-IZUMO1 interaction blocking activity as assessed by AVidity-based EXtracellular Interaction Screening (AVEXIS). Therefore, further research is required to determine the mechanism of action of these two fertilization inhibitors.
m-Quinodimethane-based fused-ring triplet hydrocarbons (2023)

Abstract Hydrocarbons with open-shell singlet and triplet ground states have long been studied. In contrast to studies of Kekulé hydrocarbons with an open-shell singlet ground state, studies of non-Kekulé and Kekulé hydrocarbons with a triplet ground state are quite limited, and no hydrocarbon with a triplet ground state has been isolated as single crystals. In this review, our work on the synthesis, isolation, and properties of m -quinodimethane-based non-Kekulé polycyclic hydrocarbon, a kinetically stabilized triangulene derivative, and Kekulé polycyclic hydrocarbon, a kinetically stabilized bisdibenzo[3,4:5,6]cyclohepta[1,2- a :2,1- d ]benzene derivative, are described. Triplet ground states of these hydrocarbons were experimentally confirmed by ESR and magnetic measurements, and these are the first example of polycyclic hydrocarbons with a triplet ground state whose structures were characterized by X-ray crystal structural analysis. These studies will enable the development of various polycyclic hydrocarbon multi-radicals with high spin multiplicity.
Vitrification and Nanowarming of Kidneys (2021)

Vitrification can dramatically increase the storage of viable biomaterials in the cryogenic state for years. Unfortunately, vitrified systems ≥3 mL like large tissues and organs, cannot currently be rewarmed sufficiently rapidly or uniformly by convective approaches to avoid ice crystallization or cracking failures. A new volumetric rewarming technology entitled "nanowarming" addresses this problem by using radiofrequency excited iron oxide nanoparticles to rewarm vitrified systems rapidly and uniformly. Here, for the first time, successful recovery of a rat kidney from the vitrified state using nanowarming, is shown. First, kidneys are perfused via the renal artery with a cryoprotective cocktail (CPA) and silica-coated iron oxide nanoparticles (sIONPs). After cooling at -40 °C min<sup>-1</sup> in a controlled rate freezer, microcomputed tomography (µCT) imaging is used to verify the distribution of the sIONPs and the vitrified state of the kidneys. By applying a radiofrequency field to excite the distributed sIONPs, the vitrified kidneys are nanowarmed at a mean rate of 63.7 °C min<sup>-1</sup> . Experiments and modeling show the avoidance of both ice crystallization and cracking during these processes. Histology and confocal imaging show that nanowarmed kidneys are dramatically better than convective rewarming controls. This work suggests that kidney nanowarming holds tremendous promise for transplantation.
Performance modeling for virtual network based service provisioning (2001)

One of the goals of network service providers (SP) is to provision efficiently value-added networking services to their end users. The services may have different duration, connectivity, and quality of service (QoS) requirements. An SP meets such requirements by exploiting various routing and management mechanisms for edge and core networks. We focus on network services that require the establishment of virtual networks (VNs). This paper considers a VN creation architecture based on multi-protocol label switching (MPLS). MPLS is one approach for implementing VNs that enables programmability for network infrastructure and hence the creation of value-added services. An analytic performance model is developed to assess the scalability of the architecture as the underlying physical network infrastructure evolves. The model we present describes only coarse features of the architecture, yet offers insights regarding its performance and scalability. In particular we assess the impact of a flat versus hierarchical MPLS mechanism on the response time of service creation.
The effects of Total Ionizing Dose irradiation on supercapacitors deployed in nuclear decommissioning environments (2020)

The effects of Total Ionizing Dose (TID) on electrical components is a key parameter to evaluate the life span of wireless sensor nodes for possible deployment in nuclear decommissioning environments. The aim of this study was to experimentally evaluate the effects of TID on capacitance, internal resistance and the self-discharge characteristic of 100 F supercapacitors. An automated test circuit was designed and assembled to charge and discharge the supercapacitors. The supercapacitors were irradiated using a Co-60 γ ray radiation source and the voltage across the supercapacitor terminals, charging current and discharging current were monitored and logged to calculate the capacitance during the irradiation process. Measurements of internal resistance and self-discharge characteristic were performed before and after the irradiation to examine the effects of exposure to γ radiation on these electrical properties. The experimental results show negligible effects on the capacitance of supercapacitors exposed to a maximum dose of 40 kGy. The internal resistance and self-discharge characteristics were not affected by TID up to 89 kGy. These results demonstrate that supercapacitors are a suitable technology to design an Energy Storage System to be deployed in the majority of nuclear decommissioning environments.
Hematologic Disorders following Prolonged Use of Intravenous Fat Emulsions in Children (1986)

Abnormalities in hematologic parameters, associated with prolonged utilization of intravenous fat emulsion (IVFE), were observed in seven children on long-term cyclic total parenteral nutrition (TPN). All patients were receiving IVFE (Intralipid 20%) 1 to 2 g/kg/24 hr as part of TPN, during 3 to 18 months. Recurrent thrombocytopenia occurred in all seven patients. Platelet lifespan, as measured with 111 Indium was reduced. Sea-blue histiocytes containing granulations and hemophagocytosis were seen on bone marrow smears. Scans taken after injection of autologous erythrocytes labeled with technetium-99 showed bone marrow sequestration of these cells. Taken together, this constellation of hematologic abnormalities suggests that long-term IVFE administration induces hyperactivation of the monocyte-macrophage system.
The structure of Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase‐dihydropteroate synthase reveals the basis of sulfa resistance (2019)

The clinical efficacy of sulfa drugs as antimalarials has declined owing to the evolution of resistance in Plasmodium falciparum ( Pf ) malaria parasites. In order to understand the basis of this resistance and to design more effective antimalarials, we have solved 13 structures of the bifunctional enzyme 6‐hydroxymethyl‐7,8‐dihydropterin pyrophosphokinase (HPPK)–dihydropteroate synthase (DHPS) from wild‐type (WT) P. falciparum and sulfa‐resistant mutants, both as apoenzyme and as complexes with pteroate (PTA) and sulfa derivatives. The structures of these complexes show that PTA, which effectively inhibits both the WT and mutants, stays in active sites without steric constraint. In contrast, parts of the sulfa compounds situated outside of the substrate envelope are in the vicinity of the resistance mutations. Steric conflict between compound and mutant residue along with increased flexibility of loop D2 in the mutants can account for the reduced compound binding affinity to the mutants. Kinetic data show that the mutants have enhanced enzyme activity compared with the WT. These Pf DHPS structural insights are critical for the design of novel, substrate envelope–compliant DHPS inhibitors that are less vulnerable to resistance mutations. Databases The data reported in this paper have been deposited in the Protein Data Bank, www.wwpdb.org . PDB ID codes: 6JWQ for apoWT; 6JWR , 6JWS , and 6JWT for PTA complexes of WT, A437G (3D7), and V1/S; 6JWU , 6JWV , and 6JWW for STZ‐DHP complexes of WT, 3D7, and V1/S; 6JWX , 6JWY , and 6JWZ for SDX‐DHP complexes of WT, 3D7, and W2; 6KCK , 6KCL , and 6KCM for Pterin/ p HBA complexes of WT, TN1, and W2.
Pelvic Autonomic Nerve Mapping Around the Prostate by Intraoperative Electrical Stimulation With Simultaneous Measurement of Intracavernous and Intraurethral Pressure (2006)

The course of the cavernous nerves did not always agree with the surgically identified neurovascular bundle. The distribution of cavernous nerves was wider than our image of the neurovascular bundle and it existed on the rectal wall posterolateral, apart from the neurovascular bundle rather than the neurovascular bundle itself. The surgically identified neurovascular bundle contained the nerve fibers contributing to urinary continence.
Heterocyclic quinones. XVII. A new in vivo active antineoplastic drug: 6,7-bis(1-aziridinyl)-4-[[3-(N,N-dimethylamino)propyl]amino]-5,8-quinazolinedione (1991)

A series of heterocyclic quinones, 6-substituted and 6,7-disubstituted 4-(alkylamino)-5,8-quinazolinediones, have been synthesized in order to evaluate their in vitro cytotoxicity on L1210 leukemia cells. Among 14 derivatives that have been prepared and studied for the structure-activity relationship, the most potent cytotoxic compound on L1210 leukemia cells was the 6,7-bis(1-aziridinyl)-4-[[3-(N,N-dimethylamino)propyl]amino]-5,8- quinazolinedione (24). This compound has been tested with the use of a cell-image processor on MCF-7 human mammary and HBL human melanoma cell lines. The results show that compound 24 influences cell proliferation and blocks both cells lines in the S phase. In vivo antineoplastic activity of compound 24 has been demonstrated on a broad spectrum of murine experimental models, but it was found highly toxic and produced long-delayed deaths.
Chronic Pain of Osteoarthritis: Considerations for Selecting an Extended-Release Opioid Analgesic (2008)

Chronic/persistent pain due to osteoarthritis is one of the most common pain conditions affecting Americans today. Inadequate pain relief or dissatisfaction with current treatments is a source of frustration and suffering for patients with chronic/persistent pain. The requirement of multiple doses of commonly used analgesics to maintain adequate pain relief is inconvenient for many patients with osteoarthritis-related chronic pain. The several extended-release opioid analgesics that have been developed may provide an opportunity for improved patient convenience; however, clinicians must consider adverse event profiles, pharmacokinetics, abuse potential, and controlled substance-scheduling status of extended-release opioid analgesics. The purpose of this review is to highlight the efficacy and safety of extended-release opioid analgesics utilized in the management plan of chronic pain due to osteoarthritis.
Experimental Study of Design Parameters in Silicon Micropillar Array Solar Cells Produced by Soft Lithography and Metal-Assisted Chemical Etching (2012)

Solar cells, consisting of core-shell p-n junction silicon micropillars on a thin membrane fabricated using soft lithography and metal-assisted chemical etching, are studied as a function of geometrical designs. Significant enhancement in absorption rate is found without much dependence on the pillar diameters in the range of 0.5-2 μm. However, the short-circuit current increases continuously with diameter, which is inversely proportional to the total surface area for a fixed diameter/pitch pillar array. This study provides unambiguous evidence that surface recombination is the dominant loss mechanism in nanowire- or micropillar-based solar cells.
Tributylstannylated silicic acid as precursor of organofunctionalized silica gel (1998)

Tributylstannylated silicic acid (TBSA), which was regarded as a protected polymeric silanol against self-condensation to give silica gel, was newly prepared from the reaction of water glass (WG) and bis(tributyltin) oxide (TBO). The ratios of Si/Sn contained in TBSA were determined by gravimetric analysis to be in the range of 2–3. The gelation of TBSA in usual organic solvents such as hexane, benzene, and dichloromethane was not observed over 3 weeks. In addition, TBSA was shown to be a convenient precursor for the preparations of silica gel modified with organofunctional groups. From the reaction of TBSA with trimethoxysilanes and aromatic alcohols, the silica gels havingorganofunctional groups were obtained with the elimination of a tributyltin group. © 1998 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 36: 655–663, 1998
Hydrosilanes Are Not Always Reducing Agents for Carbonyl Compounds, II: Ruthenium‐Catalyzed Deprotection of tert‐Butyl Groups in Carbamates, Carbonates, Esters, and Ethers (2010)

Abstract Hydrosilanes act as a reagent to cleave the C–O bond of O t Bu groups in carbamates, carbonates, esters, and ethers by catalysis of a triruthenium cluster. The reaction offers a novel deprotection method for O t Bu groups under neutral conditions, showing unique selectivities that have never been accomplished with conventional Brønsted or Lewis acidic promoters. Possible mechanisms for C–O cleavage are discussed on the basis of NMR spectroscopic analysis.
Transient electron energy-loss spectroscopy of optically stimulated gold nanoparticles using picosecond pulsed electron beam (2022)

Ultrafast phenomena in gold nanotriangles (AuNTs) were investigated using a transient electron energy-loss spectroscopy (TEELS) technique under irradiation from a 150-fs pulse laser with a wavelength of 780 nm. This investigation was conducted using a time-resolved transmission electron microscopy method that was developed to measure the dynamics of nanomaterials. Enhancement of the intensity and energy-width broadening of the energy loss were observed at the EEL peaks associated with surface and bulk plasmons on the AuNTs. The TEELS measurement revealed two decay processes of 7.8 ps and longer than 100 ps that compensate for relaxation times of excited surface plasmons using transient absorption spectroscopy. The results show that the bulk and surface plasmons have the same time evolution, i.e., that the excited electrons on the surface and in the bulk have the same relaxation processes in both electron–phonon and phonon–phonon interactions. The time evolution of electronic and lattice temperatures was also estimated based on the measured relaxation time using a two-temperature model, which revealed the volume expansion of the AuNTs and clarified the energy shifts of plasmons. Details of excited electrons in nanoparticles investigated via plasmon energy loss are expected to facilitate improvement in the performance for energy harvesting of photons in nanostructure-controlled materials.