gebhart/patrepeval huggingface dataset:
Rapid appearance of newly synthesized phosphatidylethanolamine at the plasma membrane. (1983)

We have measured the movement of newly synthesized phosphatidylethanolamine (PE) molecules from sites of intracellular synthesis to the plasma membrane in cultured V79 Chinese hamster fibroblasts. Plasma membrane PE was distinguished from intracellular PE by its derivatization with an amino-reactive reagent, trinitrobenzene sulfonic acid, under nonpermeating conditions. Within minutes after the addition of radiolabeled precursors of PE to the culture medium, radiolabeled PE appeared at the plasma membrane. The fraction of radiolabeled PE molecules appearing at the plasma membrane increased rapidly over a 2-h period and then increased very slowly for several days to a constant specific radioactivity. By measuring the release of radiolabeled secretory proteins, we determined that the transport of newly synthesized proteins to the cell surface occurred more slowly than the transport of PE. Preincubation of cells with either cytochalasin B, cytochalasin D, colchicine, oncobendazole, sodium azide, 2-deoxyglucose, dinitrophenol, p-trifluoromethoxyphenylhydrazone, or monensin did not block the transport of de novo synthesized PE; however, incubation of cells in culture medium at 2 degrees C effectively halted the appearance of new PE molecules at the plasma membrane. When cells which had been incubated at 2 degrees C were warmed, PE molecules from intracellular PE pools once again began to appear at the plasma membrane. These results suggest that the rapid transport of newly synthesized PE molecules to the plasma membrane occurs by a mechanism independent of that used for the transport of newly synthesized proteins.
Psychoacoustic abilities of subjects with unilateral and bilateral cochlear hearing impairments and their relationship to the ability to understand speech. (1989)

This paper is concerned with deficits in the ability to distinguish sounds, which accompany hearing loss of cochlear origin, and with the relationship of those deficits to the ability to understand speech in quiet and in background noise. Nine subjects with moderate unilateral cochlear hearing loss and 6 with moderate bilateral cochlear hearing loss took part in a series of psychoacoustic and speech perception tests. The impaired ears showed deficits in several of the psychoacoustic tests, including: detection of temporal gaps in bands of noise; frequency discrimination of pure tones; frequency discrimination of complex tones; and frequency selectivity as measured by the masking of tones by notched noise. The impaired ears showed near-normal performance in the detection of changes in intensity and for detecting temporal gaps in sinusoidal signals. Speech reception thresholds (SRTs--defined as the level of speech required for 50% intelligibility) were measured both in quiet and in speech-shaped noise, and were invariably higher for the impaired than for the normal ears. Correlational analysis, principal-components analysis and multiple-regression analysis were used to explore the relationships between the psychoacoustic measures and the SRTs. The results suggest that SRTs in quiet are determined primarily by absolute thresholds as measured by the pure-tone audiogram. SRTs in noise are related more to supra-threshold discrimination abilities, such as the detection of temporal gaps in noise and the frequency discrimination of pure and complex tones, and to age. Possible clinical applications of the results are briefly discussed.
Experimental verification of row-by-row variable V/sub DD/ scheme reducing 95% active leakage power of SRAM's (2005)

Low-power SRAM has become a critical component in recent VLSI systems. This paper reports an SRAM reducing 95% of active leakage power. The SRAM is successfully implemented and reliably measured for the first time, with self-aligned timing generation to avoid malfunction during V/sub DD/ transition. The cycle time overhead is 9%, and the area overhead is 3.5%.
LD-Aminopterin in the Canine Homologue of Human Atopic Dermatitis: A Randomized, Controlled Trial Reveals Dosing Factors Affecting Optimal Therapy (2014)

Once-weekly LD-aminopterin was safe and efficacious in canine AD. Twice-weekly dosing negated efficacy despite having the same daily and weekly dose as effective once-weekly regimens. Optimal dosing in this homologue of human AD correlated with the concentration-selective accumulation of AICAR in vitro, consistent with AICAR mediating LD-aminopterin efficacy in AD.
Suzuki−Miyaura Coupling of Aryl Carbamates, Carbonates, and Sulfamates (2009)

The first Suzuki-Miyaura cross-couplings of carbamates, carbonates, and sulfamates is described. The method provides a powerful means of using simple derivatives of phenol as precursors to polysubstituted aromatic compounds, as exemplified by a concise synthesis of the anti-inflammatory drug flurbiprofen.
Immune Suppression By High-Density Neutrophils Predicts Short Progression-Free Survival in Multiple Myeloma (2016)

Abstract Background and Objective : Our previous work showed that neutrophils sedimenting on top of red cells after density gradients, defined as high-density neutrophils (HDN) are emerging as pro-tumoral players with immunesuppressive properties, by depleting the surrounding microenvironment from arginine (arg) and tryptophan (trp) through increased expression of arginase (Arg-1) and 2-3 indoleamine deoxygenase (IDO), but little is known about their clinical relevance in Multiple Myeloma (MM). Experimental design : We integrated two distinct unbiased approaches. First, we assessed gene expression profiles (GEP) and functional evaluation of HDN sorted from 60 newly diagnosed MM patients, 30 patients with MGUS, and 30 healthy subjects.. Second, we adopted ultra-high performance liquid and gas chromatography followed by mass spectrometry (UHPLC/GC-MS) on an independent series of 167 samples of bone marrow (BM) and peripheral plasma collected ad hocfrom 125 individuals, comprising newly diagnosed MM, MGUS, smoldering MM, and age-matched healthy volunteers (n=29). Progression-free survival in newly diagnosed MM patients was correlated respectively to HDN functional and expression features or metabolic profiles in the two series. Results : GEP analyses disclosed that, compared to MGUS and HD, MM-HDN showed increased expression of Arg-1, IDO-1 and the angiogenic factor PROK-2/BV8 , confirmed by qRT-PCR and immunofluorescence. Indeed, MM-HDN (but not MGUS- and HD-HDN) revealed immunosuppressive activity in co-cultures with allogeneic T-cells, reverted by the selective Arg-1 inhibitor nor-NOHA. In addition, circulating Arg-1 in serum was higher in MM (169.5 ± 17.6) than MGUS (93.2 ± 10.4, p=0.0028) and healthy subjects (70.1 ± 16.5 ng/ml, p=0.0017). In MM patients there was a progressive increase from ISS stage I through III (p=0.014). High Arg-1 in MM patients' sera at diagnosis predicted clinical outcome after first line therapy, being associated to shorter progression-free survival (respectively 22.4 months versus unreached median, p=0.01). Metabolic profiling independently identified the same immunosuppressive Arg-1-urea-arginine (arg) and tryptophan (trp)-kinurenine-IDO pathways, with arg and trp significantly reduced along MM evolution. Lower levels of arg were associated to shorter PFS (37.2 versus 17.6 months, p=0.023). We then tested if aminoacid depletion (arg and trp) favors PCs growth. When exposed to trp-free or arg-free medium, human MM cell lines expressed more CHOP, p62, m-TOR and Blimp-1 (markers respectively of autophagy induction and immunoglobulin production); conversely, p62 silencing reduced Blimp-1, confirming the important role of p62-dependent mechanism in supporting PCs fitness. Conclusion : In MM, HDN are immunosuppressive due to increased Arg-1 and IDO expression. We propose the arginine/Arg-1 axis as a novel potential biomarker for MM prognosis. Disclosures Ciceri: MolMed SpA: Consultancy.
Preservation of topoisomerase genetic sequences during in vivo and in vitro development of high-level resistance to ciprofloxacin in isogenic Stenotrophomonas maltophilia strains (2005)

The increase of ciprofloxacin MICs in in vivo and in vitro isogenic S. maltophilia mutant strains was not related to quinolone resistance determining region mutations. Highly effective efflux mechanisms might preserve topoisomerase targets from a ciprofloxacin challenge in S. maltophilia.
Regional Anesthesia Decreases Inpatient But Not Outpatient Opioid Demand in Ankle and Distal Tibia Fracture Surgery (2022)

<i>Level III: Retrospective, therapeutic cohort study</i>.
Special Section Guest Editorial: Advances in Retinal Imaging (2019)

The guest editorial provides an introduction to the Special Section on Advanced Retinal Imaging: Instrumentation, Methods, and Applications.
Expression, purification from inclusion bodies, and crystal characterization of a transition state analog complex of arginine kinase: A model for studying phosphagen kinases (1997)

Phosphagen kinases catalyze the reversible transfer of a phosphoryl group between guanidino phosphate compounds and ADP, thereby regenerating ATP during bursts of cellular activity. Large quantities of highly pure arginine kinase (EC 2.7.3.3), the phosphagen kinase present in arthropods, have been isolated from E. coli, into which the cDNA for the horseshoe crab enzyme had been cloned. Purification involves size exclusion and anion exchange chromatographies applied in the denatured and refolded states. The recombinant enzyme has been crystallized as a transition state analog complex. Near complete native diffraction data have been collected to 1.86 A resolution. Substitution of a recombinant source for a natural one, improvement in the purification, and data collection at cryo temperatures have all yielded significant improvements in diffraction.
Phylogenomic analysis of the GIY-YIG nuclease superfamily (2006)

An evolutionary classification of the GIY-YIG superfamily is presented for the very first time, along with the structural annotation of all (sub)families. It provides a comprehensive picture of sequence-structure-function relationships in this superfamily of nucleases, which will help to design experiments to study the mechanism of action of known members (especially the uncharacterized ones) and will facilitate the prediction of function for the newly discovered ones.
A unified access to extract knowledge from heterogeneous Web archives (2001)

This paper proposes the integration of tools to provide unified access to remote and heterogeneous archives, the contents of which can be grouped under the same subject, and which have been integrated to allow the user to navigate and conduct thematic searches. The information sources are locally frequently modified, added to, and removed, therefore attention has been paid to the permanence of their references. Source interoperability is supported at language, protocol and schema levels. The architecture is based on a new common schema of the archives which is defined in new representation and query languages on the basis of an ontology to avoid misunderstanding and ambiguity.
Intergrated Microchemical Analysis System Using DS2 Penetrator Technology for the Enantiomeric Detection of Amino Acids (1999)

Any strategy for investigating whether abiotic and/or biotic organic molecules are present on Mars and the search for biosignatures should focus on compounds which are readily synthesized under plausible prebiotic conditions, play an essential role in biochemistry as we know it and have properties such as chirality (handedness) which can be used to distinguish between abiotic vs. biotic origins (1). Amino acids are one of the few compound classes that fulfill all these requirements. They are synthesized in high yields in prebiotic simulation experiments, are one of the more abundant types of organic compounds present in carbonaceous meteorites and only the L-enantiomers are used in the proteins and enzymes in life on Earth.
ACE: Adaptive Confusion Energy for Natural World Data Distribution (2019)

With the development of deep learning, standard classification problems have achieved good results. However, conventional classification problems are often too idealistic. Most data in the natural world usually have imbalanced distribution and fine-grained characteristics. Recently, many state-of-the-art approaches tend to focus on one or another separately, but rarely on both. In this paper, we introduce a novel and adaptive batch-wise regularization based on the proposed Adaptive Confusion Energy (ACE) to flexibly address the nature world distribution, which usually involves fine-grained and long-tailed properties at the same time. ACE increases the difficulty of the training process and further alleviates the overfitting problem. Through the datasets with the technical issue in fine-grained (CUB, CAR, AIR) and long-tailed (ImageNet-LT), or comprehensive issues (CUB-LT, iNaturalist), the result shows that the ACE is not only competitive to some state-of-the-art on performance but also demonstrates the effectiveness of training.
Micro/nanotechnologies to improve concentrated photovoltaics systems (2019)

International audience
Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma (2015)

NUSAP1 may be a crucial biomarker for OSCC. Moreover, down-regulated NUSAP1 expression suppresses tumor proliferation and also enhances anti-tumor effect of PTX by activating apoptotic pathways. Thus, the present study strongly suggests that regulating NUSAP1 expression should contribute to the therapy for OSCC.
Inactivation of the retinoblastoma susceptibility protein is not sufficient for the transforming function of the conserved region 2-like domain of simian virus 40 large T antigen (1995)

Simian virus 40 large T antigen interacts with three cellular proteins, pRb, p107, and p130, through a common binding site on the T antigen protein called the E1A conserved region 2-like (CR2-like) domain. Mutations in this domain inactivate the transforming activity of large T antigen. Since these mutations have been demonstrated to abolish binding to pRb and p107, and presumably therefore affect binding to p130, assessment of the relative roles of these three proteins in transformation of rodent fibroblasts by T antigen has been difficult. We have examined the role of T antigen-pRb interactions in transformation. We have introduced a mutant T antigen, which is unable to bind any of these three proteins, into primary mouse fibroblasts derived from the embryos of mice in which the Rb gene encoding the retinoblastoma protein had been disrupted. This mutant is unable to transform the Rb-negative fibroblasts, indicating that inactivation of pRb is not the sole function of the CR2-like domain in the induction of transformation of mouse fibroblasts by simian virus 40.
One-Chip Integrated Near-Field Thermophotovoitaic Devices Using Intermediate Transparent Substrates (2019)

We develop one-chip near-field thermophotovoltaic devices, where thin-film thermal emitters (> 1000K) and solar cells are integrated to the top and bottom of intermediate substrates with a sub-wavelength gap (< 150nm), realizing 10-fold enhancement in the photocurrent. © 2019 The Author(s)
Preparation and Characterization of Grain Boundary Josephson Junction from Amorphous Thin Film (1991)

To prepare the grain boundary Josephson junction, Y-Ba-Cu-O amorphous thin film was first etched into a bridge shape and then annealed at a high temperature, 990°C, to form grain boundaries. The bridge thus prepared showed clear Shapiro steps under the irradiation of microwaves at 4.2 K. Grains of the films sensitively varied their structures, sizes and electric properties with annealing temperature. The grains annealed at a lower temperature, 960°C, were as small as a few micrometers or less. Large grains appeared when the annealing temperature became higher, and the higher the annealing temperature, the greater the number of large grains, untill 990°C. The bridge of 5 µm in width that had grains over 10 µm in size exhibited a.c. Josephson effect, but the effect was not observed with the bridge which consisted of only small grains of a few µm.
The heart arrhythmia-linked D130G calmodulin mutation causes premature inhibitory autophosphorylation of CaMKII (2021)

The Ca<sup>2+</sup>/calmodulin (CaM)-dependent kinase II (CaMKII) is well known for transmitting Ca<sup>2+</sup>-signals, which leads to a multitude of physiological responses. Its functionality is believed to involve CaMKII holoenzyme dynamics where trans-autophosphorylation of the crucial phosphorylation site, T286 occurs. Phosphorylation of this site does not occur when stimulated exclusively with the arrhythmia associated D130G mutant form of CaM in vitro. Here, we present evidence that the loss-of-CaMKII function correlates with premature phosphorylation of its inhibitory phosphosite T306 in CaMKIIα and T307 in CaMKIIδ as this site was up to 20-fold more phosphorylated in the presence of D130G CaM compared to wildtype CaM. Indeed, changing this phosphosite to a non-phosphorylatable alanine reversed the inhibitory effect of D130G both in vitro and in live cell experiments. In addition, several phosphosites with so far undescribed functions directing the Ca<sup>2+</sup>-sensitivity of the CaMKII sensor were also affected by the presence of the D130G mutation implicating a role of several additional autophosphosites (besides T286 and T306/T307) so far not known to regulate CaMKII Ca<sup>2+</sup> sensitivity. Furthermore, we show that introducing a D130G mutation in the CALM2 gene of the P19CL6 pluripotent mouse embryonic carcinoma cell line using CRISPR/Cas9 decreased the spontaneous beat frequency compared to wildtype cells when differentiated into cardiomyocytes supporting an alteration of cardiomyocyte physiology caused by this point mutation. In conclusion, our observations shed for the first time light on how the D130G CaM mutation interferes with the function of CaMKII and how it affects the beating frequency of cardiomyocyte-like cells.
Design and development of the spinning mode synthesizer (1973)

Design and development of a flexible source of spinning modes which is capable of generating independent spinning waves of controlled complexity and spin speed without the introduction of broad band elements is reported. These features were accomplished through the use of eight commercial loudspeakers located in an equally spaced circular array with diameter of 11 inches and properly phased so that the system could generate a spinning wave. The constructed apparatus was tested in an anechoic environment and found capable of generating a plane, one and two lobed spinning wave of high quality with a sound pressure level of 120 db and at frequencies ranging from 1500 to 2500 Hz at a distance of 4 ft in the far field. The wave speeds investigated varied from 8000 to 18000 rad/sec which represent supersonic peripheral speeds.
Temel Bilimlerin Tekstil ve Moda Üzerine İzdüşümü: Nanoteknoloji Ve Şarj Edilebilir Kumaş Örneği - II. Kısım (2019)

This proceeding is the second part of a previous work, which provides readers with an overview of our multidisciplinary approach to technical textile research. It reviews the recent results of the project aiming at developing supercapacitor fabric structures. The fundamental idea of the project is based on, production of graphene nano-sheets and their application onto fabrics, growing the oxides of manganese on graphene coating and utilization of the final fabric within a suitable electrolyte as electrode material. After sample fabric weaving and battery cell preparation are briefly introduced, cyclic voltammetry and electroimpedance spectroscopy characterization of the fabricated samples are summariazed. Simple test circuit design for the measurements, basic micro-controller firmware development as well as data quality monitoring software application for visualization purposes are also introduced. Finally, the measurement results with over 800 F/g capacitance for graphite are presented.
Delayed appearance of decorin in healing burn scars (2000)

Expression of decorin in burn wounds is suppressed for about 12 months and then increases at a time when resolution of hypertrophic scarring is generally considered to occur.
Severe Pulmonary Arterial Hypertension Induced by SU5416 and Ovalbumin Immunization (2012)

The combination of chronic hypoxia and treatment of rats with the vascular endothelial growth factor (VEGF) receptor blocker, SU5416, induces pulmonary angio-obliteration, resulting in severe pulmonary arterial hypertension (PAH). Inflammation is thought to contribute to the pathology of PAH. Allergic inflammation caused by ovalbumin (OVA) immunization causes muscularization of pulmonary arteries, but not severe PAH. Whether disturbance of the immune system and allergic inflammation in the setting of lung endothelial cell apoptosis causes PAH is unknown. We investigated the effects of OVA-allergic inflammation on the development of PAH initiated by VEGF blockade-induced lung endothelial cell apoptosis. OVA-immunized rats were treated with SU5416 to induce pulmonary vascular endothelial cell apoptosis. The combination of OVA and SU5416 treatment resulted in severe angio-obilterative PAH, accompanied by increased IL-6 expression in the lungs. c-Kit(+) and Sca-1(+) cells were found in and around the lung vascular lesions. Pan-caspase inhibiton, dexamethasone treatment, and depletion of B-lymphocytes using an anti-CD20 antibody suppressed this remodeling. OVA immunization also increased lung tissue hypoxia-induced factor-1α and VEGF expression. Our results also suggest that the increased expression of hypoxia-induced factor-1α and IL-6 induced by the allergic lung inflammation may be a component of the pathogenesis of PAH.
The Omni-Kernel Architecture: Scheduler Control Over All Resource Consumption in Multi-Core Computing Systems (2014)

Clouds commonly employ virtual machine technology to leverage and efficiently utilize computational resources in data centers. The workloads encapsulated by virtual machines contend for the resources\nof their hosting machines and interference from resource sharing can cause unpredictable performance. Despite the use of virtual machine technology, the role of the operating system as an arbiter of resource\nallocation persists---virtual machine monitor functionality is implemented as an extension to an operating system and the resources provided to a virtual machine are managed by an operating system.\n\nVisibility and opportunity for control over resource allocation is needed to prevent execution by one workload from usurping resources that are intended for another. If control is incomplete, no amount of over-provisioning can compensate for it and there will\ninevitably be ways to circumvent policy enforcement. The accurate and high fidelity control over resource allocation that is required from an operating system in a virtualized environment is a new operating system challenge.\n\nThis dissertation presents the omni-kernel architecture, a novel operating system architecture designed around the basic premise of pervasive monitoring and scheduling. The architecture ensures that all resource consumption is measured, that the resource consumption resulting from a scheduling decision is attributable to an activity,\nand that scheduling decisions are fine-grained.\n\nThe viability of the omni-kernel architecture is substantiated through a faithful implementation, Vortex, for multi-core x86-64 platforms. Vortex instantiates all architectural elements of the\nomni-kernel and provides a large range of commodity operating system functionality and abstractions. Using Vortex, we experimentally corroborate the efficacy of the omni-kernel architecture by showing accurate scheduler control over resource allocation in scenarios with competing workloads. Experiments involving Apache, MySQL, and Hadoop quantify the cost of the omni-kernel pervasive monitoring and scheduling to be around 5% of CPU utilization or substantially less.