gebhart/patrepeval huggingface dataset:
The incidence of bacteria in cheese milk and Cheddar cheese and their association with flavour (1963)

Summary The numbers and types of non-starter lactic acid bacteria, lipolytic bacteria and group D streptococci in cheese milks and corresponding Cheddar cheeses have been studied and their relationship to cheese flavour discussed. Different milks, heat-treatments and starters were used, and their association with flavour investigated. The pH and fat, salt and moisture contents of the cheeses were also determined. The non-starter lactic acid flora consisted mainly of Lactobacillus casei, L. plantarum, L. brevis, L. buchnerii, Pediococcus spp. and Leuconostoc spp. Lactobacillus casei was nearly always present in the milks and cheeses, predominating in most of the cheeses, particularly in those made from milk which received the most severe of the three heat-treatments tested (160°F for 17 sec). Leuconostocs were not found in any of the cheeses. Other species sometimes occurred in approximately equal numbers to L. casei , particularly in cheeses manufactured from the milder heattreated milks. Cheeses made from milk receiving the highest heat-treatment contained fewest bacteria and scored lowest for flavour. The average flavour scores increased as the number of species contributing to the non-starter lactic acid flora at the time of tasting increased. The lipolytic organisms capable of hydrolysing butterfat consisted mainly of Gram-positive cocci, predominantly Staphylococcus saprophyticus . The group D streptococci occurred infrequently, Streptococcus faecium being the most commonly occurring species. A definite correlation was found to exist between the starters used and the flavour of the cheeses. The pH of the cheeses was associated with the flavour, but the fat, moisture and salt contents of the cheeses showed no definite effect.
Array Comparative Genomic Hybridization of Ribosomal Protein Genes In Diamond-Blackfan Anemia Patients; Evidence for Three New DBA Genes, RPS8, RPS14 and RPL15, with Large Deletion or Duplication (2010)

Abstract Abstract 1007 Diamond-Blackfan anemia (DBA) is an inherited bone marrow failure syndrome characterized by anemia, usually presenting during infancy or in early childhood. Although anemia is the most prominent feature of DBA, the disease is also characterized by cancer predisposition, growth retardation and congenital malformations, in particular craniofacial, upper limb, heart and urinary system defects, which are present in ∼30-50% of patients. We completed our large scale sequencing of 80 ribosomal protein (RP) genes and found eight of them mutated in DBA. In total, together with three RP genes identified by others, there are 11 genes mutated in ∼54% of DBA patients; RPS19, RPS24, RPS17, RPL35A, RPL5, RPL11, RPS7, RPS10, RPS26, RPL19 and RPL26. To search for moderate and large RP gene deletions and duplications we performed high resolution array comparative genomic hybridization on 80 DNA samples from DBA patients who did not have mutations in the 11 known RP genes. We found a deletion of exon 2 and 3 (4800 bp), deletion of the coding region, and duplication of exons 2 and 3 (488 bp) in RPS19 gene in three probands; three deletions of exons 1, 2 and 3 in RPS17 in three probands (2920 bp, 2886 bp and 3018 bp); and deletion of exons 1, 2 and 3 of the RPS26 gene. We also identified two deletions and a duplication in three RP genes previously not found mutated in DBA; RPS8 duplication of exon 3 (764 bp), RPS14 deletion of exons 2, 3, 4 and 5 (2568 bp) and RPS15 deletion of exon 4 (1995 bp). The deletions and duplications are being confirmed by multiplex PCRs. Interestingly, RPS14 was previously identified as a 5q- syndrome gene demonstrating that abnormality of this protein can cause both DBA and 5q- syndrome. These data bring to 14 the total number of RP genes mutated in DBA. Disclosures: No relevant conflicts of interest to declare.
Abstract 3510: The metabolomic profile of urine from prostate cancer patients using capillary electrophoresis mass spectrometry (CEMS) (2020)

Abstract Background: Prostate cancer (PCa) pathogenesis is influenced by alterations in cellular metabolism. Metabolomics measures these biochemical changes to create global tissue metabolite profiles. Urinary studies are noninvasive and can potentially identify biomarkers for PCa. Objective: We used Capillary Electrophoresis Mass Spectrometry (CE-MS) to analyze urine from men undergoing prostate biopsy to investigate their metabolomic profiles. Methods: We retrospectively analyzed 40 urine samples (20 from bx demonstrating PCa, 20 from benign) that were prospectively collected from men undergoing prostate biopsy. Analysis of charged metabolites done with Agilent CE-MS system (J Proteome Res. 2:488; 2003). Screening of potential biomarkers was performed with statistical protocols and pathway analyses. Metabolites with levels below the detection limit in all samples were excluded. Relative abundances of metabolites were normalized to levels of creatinine. Pathway analysis with MetaboAnalyst (Metabolites 9:57; 2019). Conclusions: CE-MS analysis identified several metabolic pathways that were altered in urine of men with PCa. These metabolites are involved in steroid, aromatic, microorganism and SCFA processes and warrant targeted studies which are underway in our lab. If validated, they have potential to serve as non-invasive biomarkers for PCa diagnosis and therapeutics Citation Format: Alexander Buko, Leo Cheng, Andrew Gustev, Adam Feldman. The metabolomic profile of urine from prostate cancer patients using capillary electrophoresis mass spectrometry (CEMS) [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 3510.
Nefiracetam Prevents Propofol-induced Anterograde and Retrograde Amnesia in the Rodent without Compromising Quality of Anesthesia (1998)

The ability of nefiracetam to prevent propofol-induced anterograde and retrograde amnesia is proposed to be indirect and to result from modulation of gene transcription in a manner that initiates a cascade of events involving protein synthesis leading to synaptic growth associated with the formation of the long-term memory trace.
Nose-to-brain delivery of tacrine (2007)

In the treatment of Alzheimer's disease tacrine, a cholinesterase inhibitor, is not the drug of choice due to its low oral bioavailability, extensive hepatic first-pass effect, rapid clearance from the systemic circulation, pronounced hepatotoxicity, and the availability of drugs better than tacrine in the same pharmacological class. Hence, the aim of this investigation was to ascertain the possibility of direct nose-to-brain delivery of tacrine to improve bioavailability, to avoid the first-pass effect and to minimize hepatotoxicity. Tacrine solution (TS) in propylene glycol was radiolabelled with (99m)Tc (technetium) and administered in BALB/c mice intranasally (i.n.) and intravenously (i.v.). Drug concentrations in blood and brain were determined at predetermined time intervals post dosing. Drug targeting efficiency (DTE %) and the brain drug direct transport percentage (DTP %) were calculated to evaluate the brain targeting efficiency. Brain scintigraphy imaging in rabbits was performed to ascertain the uptake of the drug into the brain. Tacrine solution was effectively labelled with (99m)Tc and was found to be stable and suitable for in-vivo studies. Following intranasal administration tacrine was delivered quickly (T(max) 60 min) to the brain compared with intravenous administration (T(max) 120 min). The brain/blood ratios of the drug were found to be higher for [(99m)Tc]TS(i.n.) compared with [(99m)Tc]TS(i.v.) at all time points. The DTE (207.23%) and DTP (51.75%) following intranasal administration suggested that part of tacrine was directly transported to brain from the nasal cavity. Rabbit brain scintigraphy imaging showed higher uptake of the drug into the brain following intranasal administration compared with intravenous administration. The results showed that tacrine could be directly transported into the brain from the nasal cavity and intranasal administration resulted in higher bioavailability of drug with reduced distribution into non-targeted tissues. This selective localization of tacrine in the brain may be helpful in reducing dose, frequency of dosing and dose-dependent side effects, and may prove an interesting new approach in delivery of the drug to the brain for the treatment of Alzheimer's disease.
Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved Resistance Profile (2016)

Bictegravir (BIC; GS-9883), a novel, potent, once-daily, unboosted inhibitor of HIV-1 integrase (IN), specifically targets IN strand transfer activity (50% inhibitory concentration [IC<sub>50</sub>] of 7.5 ± 0.3 nM) and HIV-1 integration in cells. BIC exhibits potent and selective in vitro antiretroviral activity in both T-cell lines and primary human T lymphocytes, with 50% effective concentrations ranging from 1.5 to 2.4 nM and selectivity indices up to 8,700 relative to cytotoxicity. BIC exhibits synergistic in vitro antiviral effects in pairwise combinations with tenofovir alafenamide, emtricitabine, or darunavir and maintains potent antiviral activity against HIV-1 variants resistant to other classes of antiretrovirals. BIC displayed an in vitro resistance profile that was markedly improved compared to the integrase strand transfer inhibitors (INSTIs) raltegravir (RAL) and elvitegravir (EVG), and comparable to that of dolutegravir (DTG), against nine INSTI-resistant site-directed HIV-1 mutants. BIC displayed statistically improved antiviral activity relative to EVG, RAL, and DTG against a panel of 47 patient-derived HIV-1 isolates with high-level INSTI resistance; 13 of 47 tested isolates exhibited >2-fold lower resistance to BIC than DTG. In dose-escalation experiments conducted in vitro, BIC and DTG exhibited higher barriers to resistance than EVG, selecting for HIV-1 variants with reduced phenotypic susceptibility at days 71, 87, and 20, respectively. A recombinant virus with the BIC-selected M50I/R263K dual mutations in IN exhibited only 2.8-fold reduced susceptibility to BIC compared to wild-type virus. All BIC-selected variants exhibited low to intermediate levels of cross-resistance to RAL, DTG, and EVG (<8-fold) but remained susceptible to other classes of antiretrovirals. A high barrier to in vitro resistance emergence for both BIC and DTG was also observed in viral breakthrough studies in the presence of constant clinically relevant drug concentrations. The overall virologic profile of BIC supports its ongoing clinical investigation in combination with other antiretroviral agents for both treatment-naive and -experienced HIV-infected patients.
Low Temperature Performance of Li/S Batteries (2003)

This paper relates to development of a new generation of Li/S rechargeable batteries with energy density exceeding 180 Wh/kg and rate capability comparable with aqueous systems. The cells demonstrate 5 C discharge rate at −40°C. Retained energy and capacity at −40°C exceeded 60 and 80%, respectively. At room temperature cells generate specific power up to 750 W/kg. Cells are inherently protected from overcharge at low and room temperatures by internal chemistry and can sustain 30 times overcharge. It was shown that at −40°C sulfur cathode discharge includes at least five steps, in contrast to the known two steps at room temperature. Thermal effects related to different charge-discharge steps were analyzed. Cell thermal model including Ohm and Arrhenius parts of polarization allowed simulation Ragone plots and capacity over a wide range of currents and temperatures. © 2003 The Electrochemical Society. All rights reserved.
How can a plenoptic camera be used as a metrology tool? (2019)

In this paper, we propose a method for the structured-light field (SLF) 3D measurement, involving ray calibration and phase mapping. The ray calibration is carried out to determine each light ray with metric spatio-angular parameters. Base on the ray parametric equation, the phase mapping in the SLF is developed so that spatial coordinates could be directly mapped from phase-encoding information. Then, a calibration strategy is designed to determine the mapping coefficients for each light ray, achieving high-efficiency SLF 3D reconstruction.
Niveles de ansiedad en niños victimizados sexualmente que deben declarar en juicios orales: aportes de un programa de preparación (2012)

Existe evidencia de que ninos victimas de delitos sexuales que enfrentan procesos judiciales pueden sufrir victimizacion secundaria. Diversos autores sugieren preparar a los ninos para enfrentar dicha instancia y disminuir los riesgos de victimizacion secundaria. Este articulo describe los niveles de ansiedad de ocho ninos expuestos a un programa de preparacion para ninos victimizados sexualmente que deben declarar en juicios. Se evaluo su nivel de ansiedad antes e inmediatamente despues del juicio. No se observaron diferencias significativas entre ambas mediciones. Pese a los resultados, se considera necesario que futuras investigaciones evaluen de forma rigurosa el programa y su capacidad de prevencion de la victimizacion secundaria. Finalmente, se discuten las implicancias de este tipo de programas en el sistema judicial chileno.
Triazolo(4,5-d)pyrimidines. VIII. Aryl migration of 7-aroyl-3H-1,2,3-triazolo(4,5-d)pyrimidines to 7-aryl-3H-1,2,3-triazolo(4,5-d)pyrimidines. (1985)

When mixtures of 7-chloro-3-phenyl-3H-1, 2, 3-triazolo [4, 5-d] pyrimidine (4), aromatic aldehydes (5), and a catalytic amount of 1, 3-dimethylbenzimidazolium iodide were refluxed in tetrahydrofuran (THF) in the presence of sodium hydride, 7-aroyl-3-phenyl-3H-1, 2, 3-triazolo [4, 5-d]-pyrimidines (1) were obtained in moderate yields. Although the yield was unsatisfactory, the reaction of 1 with sodium hydroxide in dimethyl sulfoxide (DMSO) resulted in aryl migration, followed by ready oxidative decarboxylation, giving 7-aryl-3-phenyl-3H-1, 2, 3-triazolo [4, 5-d]-pyrimidines (3) by way of 7-aryl-6, 7-dihydro-3-phenyl-3H-1, 2, 3-triazolo [4, 5-d] pyrimidine-7-carboxylic acids (2). When the reaction mixture of 1 with sodium hydroxide was directly subjected to potassium ferricyanide oxidation, 1 was easily convertible to 3 in good yield.
Real time network policy checking using header space analysis (2013)

Network state may change rapidly in response to customer demands, load conditions or configuration changes. But the network must also ensure correctness conditions such as isolating tenants from each other and from critical services. Existing policy checkers cannot verify compliance in real time because of the need to collect “state ” from the entire network and the time it takes to analyze this state. SDNs provide an opportunity in this respect as they provide a logically centralized view from which every proposed change can be checked for compliance with policy. But there remains the need for a fast compliance checker. Our paper introduces a real time policy checking tool called NetPlumber based on Header Space Analysis (HSA) [8]. Unlike HSA, however, NetPlumber incrementally checks for compliance of state changes, using a novel set of conceptual tools that maintain a dependency graph between rules. While NetPlumber is a natural fit for SDNs, its abstract intermediate form is conceptually applicable to conventional networks as well. We have tested NetPlumber on Google’s SDN, the Stanford backbone and Internet 2. With NetPlumber, checking the compliance of a typical rule update against a single policy on these networks takes 50-500µs on average. 1
Evidence for a Major Role of Skeletal Muscle Lipolysis in the Regulation of Lipid Oxidation During Caloric Restriction In Vivo (2001)

A lipolytic process in skeletal muscle has recently been demonstrated. However, the physiological importance of this process is unknown. We investigated the role of skeletal muscle lipolysis for lipid utilization during caloric restriction in eight obese women before and after 11 days of very low-calorie diet (VLCD) (2.2 MJ per day). Subjects were studied with indirect calorimetry and microdialysis of skeletal muscle and adipose tissue in order to analyze substrate utilization and glycerol (lipolysis index) in connection with a two-step euglycemic-hyperinsulinemic (12 and 80 mU/m(2). min) clamp. Local blood flow rates in the two tissues were determined with (133)Xe-clearance. Circulating free fatty acids and glycerol decreased to a similar extent during insulin infusion before and during VLCD, and there was a less marked insulin-induced reduction in lipid oxidation during VLCD. Adipose tissue glycerol release was hampered by insulin infusion to the same extent ( approximately 40%) before and during VLCD. Skeletal muscle glycerol release was not influenced by insulin before VLCD. However, during VLCD insulin caused a marked (fivefold) (P < 0.01) increase in skeletal muscle glycerol release. The effect was accompanied by a fourfold stimulation of skeletal muscle blood flow (P < 0.01). We propose that, during short-term caloric restriction, the reduced ability of insulin to inhibit lipids, despite a preserved antilipolytic effect of the hormone in adipose tissue, is caused by an augmented mobilization of fat from skeletal muscle, and that a physiological role of muscle lipolysis provides a local source of fatty acids.
Distinct Binding Affinities of Mac-1 and LFA-1 in Neutrophil Activation (2013)

Macrophage-1 Ag (Mac-1) and lymphocyte function-associated Ag-1 (LFA-1), two β2 integrins expressed on neutrophils (PMNs), mediate PMN recruitment cascade by binding to intercellular adhesive molecule 1. Distinct functions of LFA-1-initiating PMN slow rolling and firm adhesion but Mac-1-mediating cell crawling are assumed to be governed by the differences in their binding affinities and kinetic rates. In this study, we applied an adhesion frequency approach to compare their kinetics in the quiescent and activated states using three molecular systems, constitutively expressed receptors on PMNs, wild-type and high-affinity (HA) full-length constructs transfected on 293T cells, and wild-type and HA recombinant extracellular constructs. Data indicate that the difference in binding affinity between Mac-1 and LFA-1 is on-rate dominated with slightly or moderately varied off-rate. This finding was further confirmed when both β2 integrins were activated by chemokines (fMLF or IL-8), divalent cations (Mg(2+) or Mn(2+)), or disulfide bond lockage on an HA state. Structural analyses reveal that such the kinetics difference is likely attributed to the distinct conformations at the interface of Mac-1 or LFA-1 and intercellular adhesive molecule 1. This work furthers the understandings in the kinetic differences between Mac-1 and LFA-1 and in their biological correlations with molecular activation and structural bases.
Industrial stabilizers caprylate and N -acetyltryptophanate reduce the efficacy of albumin in liver patients (2010)

Liver failure is associated with an accumulation of toxic molecules that exert an affinity to albumin. Some of them have vasoactive activity. So far, albumin has been used as a plasma expander to improve the available circulating blood volume. However, recent studies have suggested that albumin is more effective than starch for this indication. It has not been reported yet whether the industrial stabilizers octanoate and N-acetyltryptophanate, added to albumin, occupy binding sites for vasoactive substances. The aim of this study was to determine whether the presence of the industrial stabilizers octanoate and caprylate has an impact on the effect of the albumin-binding function or circulating blood volume in patients with cirrhosis, portal hypertension, and an indication for albumin. In 25 patients who received albumin via an inline infusion filter that depleted stabilizers, there was an improvement of albumin binding, and there was less deterioration of circulating blood volume and renal function in comparison with a control group. Further studies are needed to confirm the results and identify the potential socioeconomic side effects of industrial stabilizers in commercial albumin solutions.
Data for the synthesis of β-oxopropylcarbamates from propargylic alcohols, secondary amines and CO2 catalyzed by a recyclable AgBr/ionic liquid system under ambient pressure (2018)

Data presented here are related with the research article entitled "Synthesis of ß -oxopropylcarbamates in a recyclable AgBr/ionic liquid catalytic system: An efficient assembly of CO<sub>2</sub> under ambient pressure" (Song et al., 2018) [1]. In this data article, the general synthetic procedures of ß-oxopropylcarbamates through the three-component reaction of propargylic alcohols, secondary amines and carbon dioxide (CO<sub>2</sub>) catalyzed by a recyclable AgBr/ionic liquid (IL) system under mild pressure are described. Furthermore, the process for recycling the catalysts is supplied as well. Specifically, the investigative data for the temperature, amount of ILs, reaction time as well as the state of silver in the system are also reported. Finally, all the target products are confirmed by <sup>1</sup>H NMR, <sup>13</sup>C NMR, and high-resolution mass spectroscopy (HR-MS).
Neutron-Encoded Mass Signatures for Quantitative Top-Down Proteomics (2014)

The ability to acquire highly accurate quantitative data is an increasingly important part of any proteomics experiment, whether shotgun or top-down approaches are used. We recently developed a quantitation strategy for peptides based on neutron encoding, or NeuCode SILAC, which uses closely spaced heavy isotope-labeled amino acids and high-resolution mass spectrometry to provide quantitative data. We reasoned that the strategy would also be applicable to intact proteins and could enable robust, multiplexed quantitation for top-down experiments. We used yeast lysate labeled with either (13)C6(15)N2-lysine or (2)H8-lysine, isotopologues of lysine that are spaced 36 mDa apart. Proteins having such close spacing cannot be distinguished during a medium resolution scan, but upon acquiring a high-resolution scan, the two forms of the protein with each amino acid are resolved and the quantitative information revealed. An additional benefit NeuCode SILAC provides for top down is that the spacing of the isotope peaks indicates the number of lysines present in the protein, information that aids in identification. We used NeuCode SILAC to quantify several hundred isotope distributions, manually identify and quantify proteins from 1:1, 3:1, and 5:1 mixed ratios, and demonstrate MS(2)-based quantitation using ETD.
An LCD column driver using a switch capacitor DAC (2005)

LCD column drivers have traditionally used nonlinear R-string style digital-to-analog converters (DAC). This paper describes an architecture that uses 840 linear charge redistribution 10/12-bit DACs to implement a 420-output column driver. Each DAC performs its conversion in less than 15 /spl mu/s and draws less than 5 /spl mu/A. This architecture allows 10-bit independent color control in a 17 mm/sup 2/ die for the LCD television market.
Performance characteristics of magnetic resonance cholangiography in the staging of malignant hilar strictures (2000)

MRC should be considered for planning treatment of malignant hilar strictures. Accurate depiction of high grade strictures for which endoscopic drainage is not the option of choice can preclude unnecessary invasive imaging.
FLASH-seq Low-Amplification protocol v3 (2022)

Building upon the existing Smart-seq2/3 workflows, we developed FLASH-seq (FS), a new full-length scRNA-seq method capable of detecting a significantly higher number of genes than both previous versions, requiring limited hands-on time and with a great potential for customization. FLASH-seq Low-Amplification (FS-LA), represents FS quickest iteration, generating sequencing-ready libraries in 4.5 hours by removing intermediate cleanups and QC steps and without sacrificing performance. FS-LA is the best choice when a large number of plates need to be processed in parallel.