gebhart/patrepeval huggingface dataset:
Microbial DNA Induces a Host Defense Reaction of Human Respiratory Epithelial Cells (2004)

Epithelial cells represent the initial site of bacterial colonization in the respiratory tract. TLR9 has been identified in B cells and CD 123(+) dendritic cells and found to be involved in the recognition of microbial DNA. It was the aim of the study to investigate the role of TLR9 in the host defense reactions of the respiratory epithelium. Respiratory epithelial cell lines (IHAEo(-), Calu-3) or fully differentiated primary human cells as air-liquid interface cultures were stimulated with bacterial DNA or synthetic oligonucleotides containing CpG motifs (CpG oligodeoxynucleotides). Expression of TLR9, cytokines, and human beta-defensin 2 was determined by quantitative RT-PCR or by ELISA. We found that TLR9 is expressed by respiratory epithelial cell lines and fully differentiated primary epithelial cells at low levels. Stimulation of the above-mentioned cells with bacterial DNA or CpG oligodeoxynucleotide resulted in an inflammatory reaction characterized by a dose-dependent up-regulation of cytokines (IL-6, IL-8) and human beta-defensin 2. Up-regulation of NF-kappaB in epithelial cells in response to the CpG motif containing DNA was inhibited by overexpression of a dominant negative form of MyD88. These results provide clear evidence that the human respiratory epithelium is capable of detecting microbial DNA by TLR9. The respiratory epithelium has an important function in triggering innate immune responses and therefore represents an interesting target for anti-inflammatory therapy.
Hybride Faserverbundwerkstoffe mit Kohlenstofffaserrezyklaten für Luftfahrtanwendungen (2014)

Kurzfassung Leichtbaulösungen in der Luftfahrt basieren zunehmend auf der Verwendung von Faserverbundwerkstoffen, was vor allem auf die sehr hohen spezifischen und gerichteten mechanischen Eigenschaften zurückzuführen ist. Die kohlenstofffaserverstärkten Kunststoffe (CFK) spielen dabei eine herausragende Rolle, jedoch sind Rohstoff-, Halbzeugherstellungs- und Bauteilfertigungskosten sowie der Energiebedarf zur Herstellung der Kohlenstofffasern vergleichsweise hoch. Der vorliegende Beitrag beschäftigt sich mit einer energie-, ressourcen- und kosteneffizienten Prozess- sowie Materialkombination, die die Wiederverwendung von rezyklierten Kohlenstofflangfasern für Luftfahrtkomponenten im Sekundärstrukturbereich ermöglicht.
Formation of the Structural State of a High-Strength Low-Alloy Steel upon Hot Rolling and Controlled Cooling (2019)

This article presents a review of current results of theoretical and experimental studies of the specific features of the structure that is formed in high-strength low-alloy steel in the process of hot rolling and which determines the properties of the steel. The current concepts of the physical processes that are developed at different stages of a thermomechanical treatment depending on the temperature–strain-rate regimes and chemical composition of the steel are considered. Particular attention is paid to the problems of the formation of the structural state that continue to be debated. The simulation methods of different scale level used to solve the problems of controlling structure formation at all stages of thermomechanical processing process are discussed.
Resilient modulus of asphalt concrete (1991)

The 1986 AASHTO Guide for Design of Pavement Structures incorporates the resilient modulus of pavements into the procedure. This parameter is analogous to one of the two constants used for linear elastic models of material behaviour. Mechanistic models that incorporate parameters such as the modulus are being explored in current major research efforts that may replace the AASHTO Guide in the future. At present, the Texas Department of Transportation does not routinely perform resilient modulus tests. Several approaches to measurement of the modulus of asphalt concrete have been developed in this study to provide the department with the capability to perform this test in support of both design activities and corroboration of nondestructive field testing. Techniques were developed that can be modified easily to incorporate new research findings and procedures oriented toward mechanistic design methods.
Renal Growth Factor Expression during the Early Phase of Experimental Hydronephrosis (1992)

Unilateral ureteral obstruction in the rat leads to hydronephrosis of the affected kidney and renal cell deletion through the process of apoptosis. We studied this experimental model to determine whether acute alterations in renal growth factor expression might be involved in the initiation of the apoptotic response. Northern blot analysis of hydronephrotic, contralateral and sham operated kidney polyadenylated messenger ribonucleic acid (mRNA) was performed to quantitate the expression of mRNA encoding the growth factors epidermal growth factor, transforming growth factor-beta and insulin-like growth factor II during the first 48 hours following ureteral obstruction. Although the expression of the insulin-like growth factor II mRNA was unchanged by ureteral obstruction, the expression of epidermal growth factor mRNA rapidly declined in the obstructed kidney during this period. The loss of epidermal growth factor expression was further confirmed by an immunocytochemical staining procedure that demonstrated high concentrations of epidermal growth factor in control renal tubules and a drastic loss of this staining in obstructed renal tubules. In contrast, expression of transforming growth factor-beta mRNA increased in the obstructed kidney. We believe that the altered growth factor environment of the hydronephrotic kidney might be an initiating factor in the onset of renal apoptosis associated with this condition.
A one dimensional systolic array for solving arbitrarily large least mean square problems (2003)

The design is presented of a one-dimensional systolic array for solving arbitrarily large least-mean-square problems involving QR decomposition and a triangular system of equations. The main characteristics of this array are maximization of array utilization, thus achieving a minimum global computation time, and low complexity of the resulting array, which can also be used in problems such as matrix-by-vector, matrix-by-matrix, and LU decomposition. Two systolic algorithms for QR decomposition have been designed. Their chained execution is shown.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Enhanced Mobility Support for Real-time Multimedia Services Using Cross-layer Design (2005)

The proliferation of real-time multimedia services and the tremendous growth in the number of mobile users of such services have created a need for the efficient mobility support in current IP v4 networks. Work has already been done to tackle the known issues of Mobile IPv4, such as triangular routing and inefficient mobility support for real-time services. In this paper, a novel scheme is proposed to improve the performance of Mobile IPv4 with cross-layer design, which is designed specially to support delay-sensitive SIP-based real-time multimedia services. Results from experiments show a significant improvement in the overall throughput and a reduction in the handoff latency.
Dietary Fats and Cardiovascular Disease: A Presidential Advisory From the American Heart Association (2017)

Cardiovascular disease (CVD) is the leading global cause of death, accounting for 17.3 million deaths per year. Preventive treatment that reduces CVD by even a small percentage can substantially reduce, nationally and globally, the number of people who develop CVD and the costs of caring for them. This American Heart Association presidential advisory on dietary fats and CVD reviews and discusses the scientific evidence, including the most recent studies, on the effects of dietary saturated fat intake and its replacement by other types of fats and carbohydrates on CVD. In summary, randomized controlled trials that lowered intake of dietary saturated fat and replaced it with polyunsaturated vegetable oil reduced CVD by ≈30%, similar to the reduction achieved by statin treatment. Prospective observational studies in many populations showed that lower intake of saturated fat coupled with higher intake of polyunsaturated and monounsaturated fat is associated with lower rates of CVD and of other major causes of death and all-cause mortality. In contrast, replacement of saturated fat with mostly refined carbohydrates and sugars is not associated with lower rates of CVD and did not reduce CVD in clinical trials. Replacement of saturated with unsaturated fats lowers low-density lipoprotein cholesterol, a cause of atherosclerosis, linking biological evidence with incidence of CVD in populations and in clinical trials. Taking into consideration the totality of the scientific evidence, satisfying rigorous criteria for causality, we conclude strongly that lowering intake of saturated fat and replacing it with unsaturated fats, especially polyunsaturated fats, will lower the incidence of CVD. This recommended shift from saturated to unsaturated fats should occur simultaneously in an overall healthful dietary pattern such as DASH (Dietary Approaches to Stop Hypertension) or the Mediterranean diet as emphasized by the 2013 American Heart Association/American College of Cardiology lifestyle guidelines and the 2015 to 2020 Dietary Guidelines for Americans.
Immunization with baculovirus-expressed VP4 protein passively protects against simian and murine rotavirus challenge (1990)

A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies against RRV and low levels of cross-reactive neutralizing antibodies against human strains Wa, ST3, and S2 and animal strains SA-11, NCDV, and Eb. Newborn mice suckled on VP4-immunized dams were protected against a virulent challenge dose of the simian strain RRV and against murine rotavirus Eb. The cross-reactive nature of the serum-neutralizing response generated by VP4 immunization and the protective efficacy of the immunization suggest that recombinant-expressed VP4 proteins should be considered as viable vaccine candidates.
En el lejano oeste (1995)

Receptor tyrosine kinases (RTKs) control proliferation and differentiation through their ability to bind and/or phosphorylate intracellular substrates. The repertoire of substrates recruited by different RTK is largely overlapping. It is not clear, therefore, how a cell distinguishes among signals originating from different RTKs. One possibility is that selective availability of substrates participates in the regulation of this process. To gain insight into this issue, we studied the expression pattern, during mouse embryogenesis, of the eps8 and eps15 genes, which encode two recently identified RTK substrates. Both genes are expressed from E 10 in a restricted fashion. eps8 is first expressed in frontonasal neural crest-derived cells, in the mesenchyme of branchial arches and in the liver primordium. At E 12.5-E 14, eps8 is additionally expressed in the central nervous system (CNS) in a regional restricted pattern at the met-mesencephalic transition area and in the developing submandibular salivary glands. eps15 is expressed at E 10 in the liver primordium, in the spinal ganglia and in the encephalic ganglia derived from the hindbrain neural crest. In addition, at E 12.5-E 14, eps15 is expressed, along all the CNS, in the ventricular zone where undifferentiated neuroblasts are located. The regional pattern of developmental expression of these two substrates sharply contrasts with their ubiquitous expression in adults, raising the possibility that their expression during embryogenesis is linked to selective proliferative and/or differentiative responses of specific neuroectodermal regions and body organs.
Generalizing the Optimized Gradient Method for Smooth Convex Minimization (2018)

This paper generalizes the optimized gradient method (OGM) that achieves the optimal worst-case cost function bound of first-order methods for smooth convex minimization. Specifically, this paper studies a generalized formulation of OGM and analyzes its worst-case rates in terms of both the function value and the norm of the function gradient. This paper also develops a new algorithm called OGM-OG that is in the generalized family of OGM and that has the best known analytical worst-case bound with rate $O(1/N^{1.5})$ on the decrease of the gradient norm among fixed-step first-order methods. This paper also proves that Nesterov's fast gradient method has an $O(1/N^{1.5})$ worst-case gradient norm rate but with constant larger than OGM-OG. The proof is based on the worst-case analysis called Performance Estimation Problem.
An Approach to Quantify Workload in a System of Agents (2015)

The role of humans in aviation and other domains continues to shift from manual control to automation monitoring. Studies have found that humans are often poorly suited for monitoring roles, and workload can easily spike in off-nominal situations. Current workload measurement tools, like NASA TLX, use human operators to assess their own workload after using a prototype system. Such measures are used late in the design process and can result in expensive alterations when problems are discovered. Our goal in this work is to provide a quantitative workload measure for use early in the design process. We leverage research in human cognition to define metrics that can measure workload on belief-desire-intentions based multi-agent systems. These measures can alert designers to potential workload issues early in design. We demonstrate the utility of our approach by characterizing quantitative differences in the workload for a single pilot operations model compared to a traditional two pilot model.
<title>Ultrasonic actuation for MEMS dormancy-related stiction reduction</title> (2000)

The use of ultrasonic pulses incident on surface micromachines has been shown to reduce dormancy-related failure. We applied ultrasonic pulses from the backside of a silicon substrate carrying SUMMiT processed surface micromachined rotors, used earlier as ultrasonic motors. The amplitude of the pulses was less than what is required to actuate the rotor (sub-threshold actuation). By controlling the ultrasonic pulse exposure time it was found that pulsed samples had smaller actuation voltages as compared to non-pulsed samples after twelve-hour dormancy. This result indicates that the micromachine stiction to surfaces during dormant period can be effectively eliminated, resulting in long-term stability of surface micromachines in critical applications.
Correlation between Bioassay and Protein Misfolding Cyclic Amplification for Variant Creutzfeldt-Jakob Disease Decontamination Studies (2020)

To date, approximately 500 iatrogenic Creutzfeldt-Jakob disease cases have been reported worldwide, most of them resulting from cadaveric dura mater graft and from the administration of prion-contaminated human growth hormone. The unusual resistance of prions to decontamination processes, their large tissue distribution, and the uncertainty about the prevalence of variant Creutzfeldt-Jakob disease (vCJD) in the general population lead to specific recommendations regarding identification of tissue at risk and reprocessing of reusable medical devices, including the use of dedicated treatment for prion inactivation. We previously described an <i>in vitro</i> assay, called Surf-PMCA, which allowed us to classify prion decontamination treatments according to their efficacy on vCJD prions by monitoring residual seeding activity (RSA). Here, we used a transgenic mouse line permissive to vCJD prions to study the correlation between the RSA measured <i>in vitro</i> and the <i>in vivo</i> infectivity. Implantation in mouse brains of prion-contaminated steel wires subjected to different decontamination procedures allows us to demonstrate a good concordance between RSA measured by Surf-PMCA (<i>in vitro</i>) and residual infectivity (<i>in vivo</i>). These experiments emphasize the strength of the Surf-PMCA method as a rapid and sensitive assay for the evaluation of prion decontamination procedures and also confirm the lack of efficacy of several marketed reagents on vCJD prion decontamination.<b>IMPORTANCE</b> Creutzfeldt-Jakob diseases are neurodegenerative disorders for which transmission linked to medical procedures have been reported in hundreds of patients. As prion diseases, they are characterized by an unusual resistance to conventional decontamination processes. Moreover, their large tissue distribution and the ability of prions to attach to many surfaces raised the risk of transmission in health care facilities. It is therefore of major importance that decontamination procedures applied to medical devices before their reprocessing are thoroughly validated for prion inactivation. We previously described an <i>in vitro</i> assay, which allowed us to classify accurately prion decontamination treatments according to their efficacy on variant Creutzfeldt-Jakob disease. The significance of this study is in demonstrating the concordance between previous <i>in vitro</i> results and infectivity studies in transgenic mice. Furthermore, commercial reagents currently used in hospitals were tested by both protocols, and we observed that most of them were ineffective on human prions.
File clustering using naming conventions for legacy systems (1997)

Decomposing complex software systems into conceptually independent subsystems represents a significant software engineering activity that receives considerable research attention. Most of the research in this domain deals with the source code; trying to cluster together files which are conceptually related. In this paper we propose using a more informal source of information: file names. We present an experiment which shows that file naming convention is the best file clustering criteria for the software system we are studying. Based on the experiment results, we also sketch a method to build a conceptual browser on a software system. Introduction Maintaining legacy software systems is a problem which many companies face. To help software engineers in this task, researchers are trying to provide tools to help extract the design structure of the software system using whatever source of information is available. Clustering files into subsystems is considered an important part of this ac...
Antigenicity of human T-cell leukemia virus-associated gp52: greater response in leukemia patients compared to healthy donors exposed to the virus. (1985)

Monoclonal antibody HT462 recognizes a human T-cell leukemia virus type I (HTLV-I)-associated Mr 52,000 glycoprotein (HA-gp52), which is found on the surface of HTLV-infected cells. Whether HA-gp52 is encoded by the virus or by the infected cells has not yet been established. Using monoclonal HT462 in a competitive binding assay, natural human antibodies specific for HA-gp52 were detected in 97% of the patients from the United States, the Caribbean, and Japan with adult T-cell leukemia but not in healthy donors not exposed to HTLV-I. In contrast, antibodies to HA-gp52 occur in healthy virus-exposed donors, but at a lower prevalence than that observed in patients. Among Japanese from HTLV-I-endemic areas and exposed to the virus as indicated by the presence of antibodies to disrupted HTLV-I, 93% of adult T-cell leukemia patients were also seropositive for HA-gp52 compared to only 16% of healthy individuals. Differing sensitivities in the methods of assaying antibodies to HTLV and HA-gp52 were not responsible for these observations as shown by the lack of correlation of HTLV-I antibody titer with the presence of antibody to HA-gp52. Among adult T-cell leukemia patients, antibody titers to HTLV-I and HA-gp52 also varied independently. These results indicate that HA-gp52 in humans is antigenic and correlated with disease. Detection of antibody to this protein in asymptomatic individuals may be indicative of a predisease condition.
The HCV Non-Nucleoside Inhibitor Tegobuvir Utilizes a Novel Mechanism of Action to Inhibit NS5B Polymerase Function (2012)

Tegobuvir (TGV) is a novel non-nucleoside inhibitor (NNI) of HCV RNA replication with demonstrated antiviral activity in patients with genotype 1 chronic HCV infection. The mechanism of action of TGV has not been clearly defined despite the identification of resistance mutations mapping to the NS5B polymerase region. TGV does not inhibit NS5B enzymatic activity in biochemical assays in vitro, suggesting a more complex antiviral mechanism with cellular components. Here, we demonstrate that TGV exerts anti-HCV activity utilizing a unique chemical activation and subsequent direct interaction with the NS5B protein. Treatment of HCV subgenomic replicon cells with TGV results in a modified form of NS5B with a distinctly altered mobility on a SDS-PAGE gel. Further analysis reveals that the aberrantly migrating NS5B species contains the inhibitor molecule. Formation of this complex does not require the presence of any other HCV proteins. The intensity of the aberrantly migrating NS5B species is strongly dependent on cellular glutathione levels as well as CYP 1A activity. Furthermore analysis of NS5B protein purified from a heterologous expression system treated with TGV by mass spectrometry suggests that TGV undergoes a CYP- mediated intracellular activation step and the resulting metabolite, after forming a glutathione conjugate, directly and specifically interacts with NS5B. Taken together, these data demonstrate that upon metabolic activation TGV is a specific, covalent inhibitor of the HCV NS5B polymerase and is mechanistically distinct from other classes of the non-nucleoside inhibitors (NNI) of the viral polymerase.
Differential Cardiac Histopathology in Inbred Mouse Strains Chronically Infected with Trypanosoma cruzi (1992)

Seven inbred mouse strains were examined for the presence of chronic Chagas' cardiomyopathy in postacute Trypanosoma cruzi infection. DBA/1, DBA/2, BALB/c, B10.T (6R), B10.Q, B10.D2, and B6 mice were infected for 100 days with the Brazil strain of T. cruzi. Standard histologic examination of cardiac tissue from these mice revealed the following relationship among the different strains based on the severity of observed inflammation (myocarditis): BALB/c, DBA/1, and DBA/2 were the most inflamed; B10.T (6R) and B10.Q were intermediate; and B6 and B10.D2 showed the least inflammation. Examination of these tissues for characteristics of myocardiopathy such as cell swelling, edema, vacuolization, necrosis, myocytolysis, connective tissue infiltration, and thinning of the right ventricular wall indicated a relative relationship among the different strains relative to the severity of cardiomyopathy as follows: BALB/c, DBA/2, and DBA/1 showed the most cardiopathy (pathopermissive); B10.T (6R) and B10.Q showed intermediate pathology; and B6 and B10.D2 showed the least involvement (pathoresistant). Anti-heart antibody present in the sera of all these mice showed specific reactivity in western blots to a 43-kDa glycoprotein from normal heart tissue. Also, anti-heart antibody enzyme-linked immunosorbent assay titers for all mouse strains were similar and showed no correlation with the severity of tissue damage. The fact that different inbred strains show various degrees of myocarditis and cardiomyopathy may be useful in the study of pathogenesis of chronic Chagas' disease. Results from this limited list of inbred strains suggest that background genes, rather than the major histocompatibility complex, play the major role in the expression of cardiac pathogenesis.(ABSTRACT TRUNCATED AT 250 WORDS)