gebhart/patrepeval huggingface dataset:
Hypoxia Concomitant with Serum Deprivation Induces Endothelial Progenitor Cell Death (2010)

Objective To evaluate the effects of serum deprivation (SD) and hypoxia on the bone marrow endothelial progenitor cells (EPCs) survival in vitro. Methods Rat bone marrow EPCs were exposed for 48 h to 02 deprivation, serum deprivation (SD), and prolonged (120 h) hypoxia concomitant with serum deprivation. Cell death was assessed by Live/Dead staining and image analysis. Results The EPCs death rate seemed not affected by 48 h hypoxia (P＞0.05), but affected by SD (P＜0.01). Prolonged hypoxia concomitant with SD resulted in the death of nearly all EPCs from 72 h, but this rate was remarkably reduced when with 20% fetal bovine serum (P＜0.01). Conclusion Our findings indicate that EPCs are sensitive to hypoxia/SD stimuli, while serum may be the more important factor for EPCs survival.
Thermal Stress and Strength Prediction of Diesel Engine Cylinder Head (1983)

&lt;div class="htmlview paragraph"&gt;This paper describes the research which objective is to establish the method to predict the crack initiation life of the diesel engine cylinder head caused by thermal fatigue. Both experimental and analytical researches were carried out. The experiments were to determine thermal and mechanical boundary conditions, applying a foil-type thermo-couple and a high-temperature strain gauge. FEM models for heat transfer and thermal stress analysis were prepared and refined based on the experimental results.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Finally, the authors propose a prediction method, which consists of simplified FEM analysis and evaluation of thermal fatigue strength.&lt;/div&gt;
New approach to phase and modulation resolved spectra (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew approach to phase and modulation resolved spectraEnrico. Gratton and David M. JamesonCite this: Anal. Chem. 1985, 57, 8, 1694–1697Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/ac00285a041https://doi.org/10.1021/ac00285a041research-articleACS PublicationsRequest reuse permissionsArticle Views124Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evaluation of pranthocyanidin enriched cranberry extract for carbohydrate hydrolysis enzyme inhibition (647.38) (2014)

Proanthocyanidins (PACs), found in cranberries (Vaccinium macrocarpon), are hypothesized to be natural, oral hypoglycemic agents. This study investigates the inhibition potential of cranberry powder in carbohydrate hydrolysis enzymes. The whole extract (WE) was achieved by dissolving one gram of cranberry powder in 100 mL acetone solution (acetone: water: hydrochloric acid, 70:29.9:0.01) at room temperature. The sample was differentiated into two fractions: low molecular weight phenolic enriched fraction (LMWF) and PAC enriched fraction (PACF) using LH‐20. PAC contents were evaluated by 4‐Dimethylaminocinnamaldehyde (DMAC)assay and yielded 1.35, 0.03 and 0.59 mg/mL for WE, LMWF and PACF respectively. The total phenolic contents of were determined to be 4.68, 0.60, and 2.51 mg/mL for WE, LMWF and PACF respectively. Rat α glucosidase assays showed PACF has 3 times lower inhibitory activity and LMWF has almost 2 times higher inhibitory activity, compared to WE. Sucrase inhibition assays showed PACF has lower inhibitory activity and that LMWF has almost 2 times higher inhibitory activity, compared to WE. Lastly, maltase inhibition assays showed both PACF and LMWF have lower inhibitory activity compared to WE. The results suggest cranberry has an inhibitory effect against the action of alpha‐glucosidases, which is mainly due to sucrase inhibition, and is low molecular weight phenolic dependent. Grant Funding Source : This research was supported by US Army Natick Soldier Research Engineering Center
Identification of Campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein (1995)

To facilitate discrimination between the closely related enteropathogens Campylobacter jejuni and C. coli, unique differences in antigenic surface structure were examined. A genomic library of C. jejuni 81116 was constructed in plasmid pBluescriptIISK- and expressed in Escherichia coli K-12. Rabbit hyperimmune serum raised against C. jejuni ATCC 29428 recognized a clone expressing a C. jejuni 24-kDa membrane-associated protein. Antiserum raised against sonicated recombinant E. coli expressing the 24-kDa protein reacted with C. jejuni, whereas C. coli did not react specifically. Determination of the nucleotide sequence of the DNA insert of this recombinant plasmid revealed an open reading frame encoding 214 amino acids; the gene was designated mapA; and its gene product was designated MAPA. The 18 N-terminal amino acid residues constitute a signal sequence characteristic of prokaryotic membrane lipoproteins. In a dot blot hybridization assay with a mapA probe, 120 clinical isolates of C. jejuni were unequivocally discriminated from 126 other campylobacters, including 34 C. coli isolates. A PCR test based on the mapA sequence was developed for identification of C. jejuni. A PCR product was obtained with all of the clinical isolates of C. jejuni tested from human, dog, cat, bovine calf, and chicken sources. Recombinant MAPA with an added C-terminal six-histidine tail was affinity purified and used to immunize rabbits. The rabbit anti-MAPA serum specifically recognized the protein in whole cells of C. jejuni on Western blots (immunoblots). The MAPA protein was present in all of the C. jejuni strains tested and was absent in C. coli and related campylobacters.
The First Structures of T Cell Receptors Bound to Peptide–MHC (2010)

The structure of the MHC class I molecule HLA-A2 reported in 1987 by Bjorkman et al. ([1][1]) had revealed how peptide Ags are presented to T cells: peptides are buried in the long and deep groove of the MHC molecule, flanked on each side by a long α helix. The structure immediately suggested that
Comparison of Stage and Lymph Node Ratio in Young and Older Patients with Colorectal Cancer Operated in a Tertiary Hospital in Nepal (2018)

Young patients with colorectal cancer tend to have more advanced disease. The lymph node metastasis and lymph node ratio tend to be higher in young patients.
[Prenatal diagnosis of hereditary diseases using chorionic villi]. (1986)

The paper is based on the first results of a preliminary study of the cytogenetic analysis of chorionic villi samples. Chorionic villi were obtained from 31 women undergoing artificial abortion in the first trimester of pregnancy. The tissue was used for direct chromosome preparations and sex-chromatin examinations (X and Y body). The results suggest the possibility of a simple and quick prenatal determination of fetal sex in the first trimester and the provision of a sufficient number of mitosis with well-spread chromosomes. This new method may become the method of choice in prenatal diagnosis after being thoroughly examined and verified.
A Synthetic Circuit for Mercury Bioremediation Using Self-Assembling Functional Amyloids (2017)

Synthetic biology approaches to bioremediation are a key sustainable strategy to leverage the self-replicating and programmable aspects of biology for environmental stewardship. The increasing spread of anthropogenic mercury pollution into our habitats and food chains is a pressing concern. Here, we explore the use of programmed bacterial biofilms to aid in the sequestration of mercury. We demonstrate that by integrating a mercury-responsive promoter and an operon encoding a mercury-absorbing self-assembling extracellular protein nanofiber, we can engineer bacteria that can detect and sequester toxic Hg<sup>2+</sup> ions from the environment. This work paves the way for the development of on-demand biofilm living materials that can operate autonomously as heavy-metal absorbents.
Robust low-power reconfigurable computing with a variation-aware preferential design approach (2014)

Reconfigurable hardware platforms, such as Field Programmable Gate Arrays (FPGA), are being increasingly used in diverse embedded applications. These platforms often use high-density memory array, which suffer from variation-induced parametric failures. Such failures lead to incorrect operation and hence, loss in output quality for many signal processing applications. In this paper, we propose a preferential design approach at both application mapping and circuit level, which can significantly improve output quality as well as energy efficiency for signal processing applications under large parameter variations. The proposed mapping process considers the reliability map of a memory array and maps the important operations with respect to output quality to more reliable memory blocks under performance constraint. At circuit level, we exploit the read-dominant memory access pattern in reconfigurable platforms to skew the memory cells for better read stability leading to improved quality. Such an architecture/circuit co-design approach can also tolerate increased failure rate at low operating voltage, thus facilitating low-power operation.
Tunable microelectromechanical grating in silicon (2006)

In this paper, we propose a solution for simple, fast and easily controllable way of tuning silicon gratings using Micro Electro Mechanical Systems (MEMS) to deform the grating itself. Basically the idea is to deform mechanically a silicon grating using electrostatic actuators, enabling pitch tuning over a large proportion (more than 50% is easily achievable with our approach). Moreover we can change the spacing of individual layers within the grating. A theoretical analysis and numerical simulations are presented and a first prototype is fabricated. Bragg gratings, springs and actuators are realized by silicon micro/nano machining on a silicon platform enabling full integration and passive alignment of all optical components. Applications range from ultra-sensitive displacement sensors, to telecommunications and biology.
Penetration Depth Measurements of 3DXYCritical Behavior inYBa2Cu3O6.95Crystals (1994)

We report measurements of the electromagnetic penetration depth $\ensuremath{\lambda}(T)$ in nominally pure crystals of ${\mathrm{YBa}}_{2}$${\mathrm{Cu}}_{3}$${\mathrm{O}}_{6,95}$, for temperatures close to the critical temperature ${T}_{c}$. Over the range $\frac{0.001&lt;({T}_{c}\ensuremath{-}T)}{{T}_{c}&lt;0.1}$, we find that $\ensuremath{\lambda}(T)\ensuremath{\propto}{(\frac{1\ensuremath{-}T}{{T}_{c}})}^{\ensuremath{-}y}$ with $y\ensuremath{\approx}0.33$, consistent with the critical behavior of the three dimensional $\mathrm{XY}$ model. The measured critical behavior is not affected by the presence of small amounts of Zn impurities, in agreement with the Harris criterion.
Prediction of Dissolved Gas Concentrations in Transformer Oil Based on the KPCA-FFOA-GRNN Model (2018)

The purpose of analyzing the dissolved gas in transformer oil is to determine the transformer’s operating status and is an important basis for fault diagnosis. Accurate prediction of the concentration of dissolved gas in oil can provide an important reference for the evaluation of the state of the transformer. A combined predicting model is proposed based on kernel principal component analysis (KPCA) and a generalized regression neural network (GRNN) using an improved fruit fly optimization algorithm (FFOA) to select the smooth factor. Firstly, based on the idea of using the dissolved gas ratio of oil to diagnose the transformer fault, gas concentration ratios are also used as characteristic parameters. Secondly, the main parameters are selected from the feature parameters using the KPCA method, and the GRNN is then used to predict the gas concentration in the transformer oil. In the training process of the network, the FFOA is used to select the smooth factor of the neural network. Through a concrete example, it is shown that the method proposed in this paper has better data fitting ability and more accurate prediction ability compared with the support vector machine (SVM) and gray model (GM) methods.
Electrochemical Formation of a Pt/Zn Alloy and Its Use as a Catalyst for Oxygen Reduction Reaction in Fuel Cells (2006)

The characterization of an electrochemically created Pt/Zn alloy by Auger electron spectroscopy is presented indicating the formation of the alloy, the oxidation of the alloy, and the room temperature diffusion of the Zn into the Pt regions. The Pt/Zn alloy is stable up to 1.2 V/RHE and can only be removed with the oxidation of the base Pt metal either electrochemically or in aqua regia. The Pt/Zn alloy was tested for its effectiveness toward oxygen reduction. Kinetics of the oxygen reduction reaction (ORR) were measured using a rotating disk electrode (RDE), and a 30 mV anodic shift in the potential of ORR was found when comparing the Pt/Zn alloy to Pt. The Tafel slope was slightly smaller than that measured for the pure Pt electrode. A simple procedure for electrochemically modifying a Pt-containing gas diffusion electrode (GDE) with Zn was developed. The Zn-treated GDE was pressed with an untreated GDE anode, and the created membrane electrode assembly was tested. Fuel cell testing under two operating conditions (similar anode and cathode inlet pressures, and a larger cathode inlet pressure) indicated that the 30 mV shift observed on the RDE was also evident in the fuel cell tests. The high stability of the Pt/Zn alloy in acidic environments has a potential benefit for fuel cell applications.
Discrete absorptions and emissions in crop spectra (1978)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text V. R. Rao, E. J. Brach, and A. R. Mack, "Discrete absorptions and emissions in crop spectra," Appl. Opt. 17, 493-496 (1978) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Quantitative determination of degree of conversion in photocured poly(urethane‐dimethacrylate)s by Fourier transform infrared spectroscopy (2011)

Abstract In this work, infrared spectroscopy was used to characterize the degree of double bonds conversion (DC) in six series of poly(urethane‐dimethacrylate)s, derived from dicarbamates of oligoethylene glycols monomethacrylates and aliphatic, cycloaliphatic and aromatic diisocyanates. To evaluate the degree of conversion in polymers devoid of aromatic moieties, two methods of calculation have been proposed: internal band ratio method, with carbonyl stretching vibrations as a standard, and a method involving the direct observation of the decrease in the intensity of CC stretching band. The validity of these methods has been checked on polymers with aromatic structures, by comparing the results with those obtained by the internal band ratio method applying an aromatic band as a reference. A good correlation between the degree of conversion from two internal standard methods, using aromatic band and carbonyl band as references, was obtained. It was shown that the degree of conversion increases as the number of oxyethylene units increases. Polymerization of monomers with aliphatic cores resulted in higher DC values, while those with cycloaliphatic and aromatic cores—resulted in lower DC values. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Downregulation of Rap1GAP in Human Tumor Cells Alters Cell/Matrix and Cell/Cell Adhesion (2010)

Rap1GAP expression is decreased in human tumors. The significance of its downregulation is unknown. We show that Rap1GAP expression is decreased in primary colorectal carcinomas. To elucidate the advantages conferred on tumor cells by loss of Rap1GAP, Rap1GAP expression was silenced in human colon carcinoma cells. Suppressing Rap1GAP induced profound alterations in cell adhesion. Rap1GAP-depleted cells exhibited defects in cell/cell adhesion that included an aberrant distribution of adherens junction proteins. Depletion of Rap1GAP enhanced adhesion and spreading on collagen. Silencing of Rap expression normalized spreading and restored E-cadherin, beta-catenin, and p120-catenin to cell/cell contacts, indicating that unrestrained Rap activity underlies the alterations in cell adhesion. The defects in adherens junction protein distribution required integrin signaling as E-cadherin and p120-catenin were restored at cell/cell contacts when cells were plated on poly-l-lysine. Unexpectedly, Src activity was increased in Rap1GAP-depleted cells. Inhibition of Src impaired spreading and restored E-cadherin at cell/cell contacts. These findings provide the first evidence that Rap1GAP contributes to cell/cell adhesion and highlight a role for Rap1GAP in regulating cell/matrix and cell/cell adhesion. The frequent downregulation of Rap1GAP in epithelial tumors where alterations in cell/cell and cell/matrix adhesion are early steps in tumor dissemination supports a role for Rap1GAP depletion in tumor progression.
Tuberculosis risk factors and mortality for HIV-infected persons receiving antiretroviral therapy in South Africa (2010)

In HIV-infected patients who started ART at low CD4 cell counts, tuberculosis at baseline was a predictor of death, but was not independent of other factors indicating poor baseline health status. Tuberculosis during follow-up was, in contrast, an independent predictor of death even after adjustments for baseline risk factors, including CD4 cell count and viral load. Virologic failure during ART was associated with a 55% increase in risk of tuberculosis. Thus, tuberculosis is a major marker for poor outcome both at baseline and during ART and is not completely eliminated by fully suppressive ART.
A New and Efficient Method for Synthesis of 5′-Conjugates of Oligonucleotides through Amide-Bond Formation on Solid Phase (2002)

An efficient method for synthesis of oligonucleotide 5′-conjugates through amide-bond formation on solid phase is described. Protected oligonucleotides containing a 5′-carboxylic acid function were obtained by use of a novel non-nucleosidic phosphoramidite building block, where the carboxylic acid moiety was protected by a 2-chlorotrityl group. The protecting group is stable to the phosphoramidite coupling conditions used in solid-phase oligonucleotide assembly, but is easily deprotected by mild acidic treatment. The protecting group may be removed also by ammonolysis. 5′-Carboxylate-modified oligonucleotides were efficiently conjugated on solid support under normal peptide-coupling conditions to various amines or to the N-termini of small peptides to yield products of high purity. The method is well-suited in principle for the synthesis of peptide-oligonucleotide conjugates containing an amide linkage between the 5′-end of an oligonucleotide and the N-terminus of a peptide.
DECISION SUPPORT FOR DYNAMIC CITY TRAFFIC MANAGEMENT USING VEHICULAR COMMUNICATION (2011)

In this paper, we present an integrated simulation approach featuring centralized and decentralized traffic management in urban areas. Our aim is to improve traffic flows by dynamic traffic management which is supported by vehicular communication interlinking centralized and decentralized decision making. We focus on traffic state estimation and the optimization of traffic lights as a central component to influence local traffic states, while individual traffic participants’ behavior is modeled by multiagent systems. Traffic participants achieve their individual goals by formation of groups and improving their knowledge about the road network by means of learning. Modeling of vehicular communication takes into account specific characteristics of urban areas, ensuring the realistic collection and dissemination of (de)centralized information. We provide a comprehensive microscopic traffic simulation framework featuring innovative functionality regarding dynamic traffic management, decentralized decision making as well as realistic communication modeling. To illustrate and validate our approach, we present a use case in a city scenario. Simulations are implemented based on the microscopic traffic simulator AIMSUN, which is significantly extended using the
Transport Properties and Finite Size Effects in $\beta$-Ga$_2$O$_3$ Thin Films. (2018)

Thin films of the wide band gap semiconductor $\beta$-Ga$_2$O$_3$ have a high potential for applications in transparent electronics and high power devices. However, the role of interfaces remains to be explored. Here, we report on fundamental limits of transport properties in thin films. The conductivities, Hall densities and mobilities in thin homoepitaxially MOVPE grown \mbox{(100)-orientated $\beta$-Ga$_2$O$_3$} films were measured as a function of temperature and film thickness. At room temperature, the electron mobilities ((115$\pm$10) $\mathrm{\frac{cm^2}{Vs}}$) in thicker films (> 150 nm) are comparable to the best of bulk. However, the mobility is strongly reduced by more than two orders of magnitude with decreasing film thickness ((5.5$\pm$0.5) $\mathrm{\frac{cm^2}{Vs}}$ for a 28 nm thin film). We find that the commonly applied classical Fuchs-Sondheimer model does not explain the contribution of electron scattering at the film surfaces sufficiently. Instead, by applying an electron wave model by Bergmann, a contribution to the mobility suppression due to the large de Broglie wavelength in $\beta$-Ga$_2$O$_3$ is proposed as a limiting quantum mechanical size effect.
Sp1 Increases Expression of Cyclooxygenase-2 in Hypoxic Vascular Endothelium (2000)

Cyclooxygenase-2 (COX-2) catalyzes prostaglandin synthesis from arachidonic acid and is expressed locally in aortic aneurysm and heart failure. Cellular hypoxia is also found in these conditions. We have previously shown that cox-2 is transcriptionally regulated by hypoxia in human umbilical vein endothelial cells (HUVEC) in culture via the transactivation factor NF-kappaB p65, leading to increased production of prostaglandin E(2), an inhibitor of vascular smooth muscle cell proliferation. Sp1 is a transactivation factor known to be important in the regulation of cytokine expression in association with NF-kappaB. We hypothesized that Sp1 is involved in the induction of cox-2 in hypoxic HUVEC. Electrophoretic mobility shift assays with hypoxic HUVEC nuclear protein showed that both Sp1 and the related protein Sp3 specifically bound to the cox-2 promoter. Immunoblotting demonstrated that hypoxia increased the nuclear localization of Sp1 but did not change the Sp3 content in HUVEC. Overexpression of Sp1 through transfection of HUVEC enhanced cox-2 promoter activity as measured by reporter gene expression and by the production of COX-2. The specificity of the results was confirmed by mutation of the Sp1-binding site in the cox-2 promoter construct and by reproducibility in an Sp-deficient Drosophila SL2 cell line. The regulatory role of Sp1 discovered in this work supports the concept that a mechanistic link exists between vascular cellular hypoxia and mediators of inflammation associated with aortic aneurysm and heart failure.