gebhart/patrepeval huggingface dataset:
Retinoblastoma protein positively regulates terminal adipocyte differentiation through direct interaction with C/EBPs. (1996)

To define a mechanism by which retinoblastoma protein (Rb) functions in cellular differentiation, we studied primary fibroblasts from the lung buds of wild-type (RB+/+) and null-mutant (RB-/-) mouse embryos. In culture, the RB+/+ fibroblasts differentiated into fat-storing cells, either spontaneously or in response to hormonal induction; otherwise syngenic RB-/- fibroblasts cultured in identical conditions did not. Ectopic expression of normal Rb, but not Rb with a single point mutation, enabled RB-/- fibroblasts to differentiate into adipocytes. Rb appears in murine fibroblasts to activate CCAAT/enhancer-binding proteins (C/EBPs), a family of transcription factors crucial for adipocyte differentiation. Physical interaction between Rb and C/EBPs was demonstrated by reciprocal coimmunoprecipitation, but occurred only in differentiating cells. Wild-type Rb also enhanced the binding of C/EBP to cognate DNA sequences in vitro and the transactivation of a C/EBPbeta-responsive promoter in cells. Taken together, these observations establish a direct and positive role for Rb in terminal differentiation. Such a role contrasts with the function of Rb in arresting cell cycle progression in G1 by negative regulation of other transcription factors like E2F-1.
844. A Single Vector-Dependent, Doxycycline- Regulatable, Lung Cancer Specific Oncolytic Adenovirus (2006)

Tight regulation of oncolytic adenoviruses (oAdV) represents an important requirement for their safe application. We have developed a new doxycycline (Dox)-dependent oAdV with a bidirectional expression cassette, which drives the expression of the reverse tetracycline-controlled transactivator (rtTAs-M2) from a lung tumor-specific promoter and, in the opposite direction, the expression of the adenoviral E1A gene from a second generation tetO7 sequence linked to an isolated TATA box. In H441 lung cancer cells, this oAdV showed a strictly Dox-dependent E1A expression, adenoviral replication, cell killing activity and a 450-fold induction of progeny virus production. Furthermore, the virus could be shut off again by withdrawal of Dox and, in contrast to an oAdV expressing E1A directly from the SP-B promoter, did not replicate in non-target HeLa cells. In contrast, when the isolated TATA box in the E1A expression cassette was replaced by a CMV minimal promoter, the corresponding oAdV already expressed significant amounts of E1A in the absence of the inducer. These were sufficient to prevent any Dox-dependent regulation of adenoviral replication. These results underline the importance of non-leaky E1A expression for preventing undesirable oAdV replication and demonstrate, for the first time, Dox-dependent oAdV replication from a single adenoviral vector genome.
Optimal modulation for known interference (2008)

We present a symbol-by-symbol approach to the problem of canceling known interference at the transmitter in a communication system. In the envisioned system, the modulator maps an information symbol (taken from a finite alphabet) and an interference symbol (from the complex field) onto a transmitted constellation point. Our scheme is based on joint optimization of a modulator and demodulator, subject to a constraint on the average transmit power. The demodulator picks the information symbol (as a function of the received symbol) that minimizes the average error probability. We emphasize that our focus is on transmission in a single (complex) dimension, and hence the proposed technique is a "modulation" rather than a "coding" scheme. We illustrate that the new scheme outperforms Tomlinson-Harashima precoding, which is a classical but suboptimal solution to the one-dimensional known-interference precoding problem. In our simulations, the new approach is able to perform close to the no-interference bound.
An Interview Study of Hospitalized Drug Overdose Patients (1978)

A study of 67 patients hospitalized after intentional drug overdose revealed that individuals whose average alcohol intake exceeded 20 grams per day were more likely to have ingested alcohol at the time of overdose and to have a history of acute drug ingestion. Besides having a higher rate of unemployment and more frequent dependence on welfare, the heavier drinkers were more transient and more likely to have had contact with medical facilities and legal authorities than were patients who averaged less than 20 grams of alcohol per day. More effective communication between individuals involved in the treatment of drug overdose could alert physicians to patient characteristics associated with self injury, thus allowing them to monitor closely or restrict drug therapy for high risk patients.
Atomic Force Microscopy of Proteasome Assemblies (2011)

The proteasome is the essential prime protease in all eukaryotes. The large, multisubunit, modular, and multifunctional enzyme is responsible for the majority of regulated intracellular protein degradation. It constitutes a part of the multienzyme ubiquitin-proteasome pathway, which is broadly implicated in recognition, tagging, and cleavage of proteins. The name "proteasome" refers to several types of protein assemblies sharing a common catalytic core particle. Additional protein modules attach to the core, regulate its activities, and broaden its functional capabilities. The structure of proteasomes has been studied extensively with multiple methods. The crystal structure of the core particle was solved for several species. However, only a single structure of the core particle decorated with PA26 activator has been determined. NMR spectroscopy was successfully applied to probe a much -simpler, archaebacterial type of the core particle. In turn, electron microscopy was very effective in exploring the spatial arrangement of many classes of assemblies. Still, the makeup of higher-order -complexes is not well established. Besides, the crystal structure provided very limited information on proteasome molecular dynamics. Atomic force microscopy (AFM) is an ideal technique to address questions that are unanswered by other approaches. For example, AFM is perfectly suited to study allosteric regulation of proteasome, the role of protein dynamics in enzymatic catalysis, and the spatial organization of modules and subunits in assemblies. Here, we present a method that probes the conformational diversity and dynamics of yeast core particle using the oscillating mode AFM in liquid. We are taking advantage of the observation that the tube-shaped core particle is equipped with a swinging gate leading to the catalytic chamber. We demonstrate how to identify distinct gate conformations in AFM images and how to characterize the gate dynamics controlled with ligands and disturbed by mutations.
SSDAlloc: hybrid SSD/RAM memory management made easy (2011)

We introduce SSDAlloc, a hybrid main memory management system that allows developers to treat solid-state disk (SSD) as an extension of the RAM in a system. SSDAlloc moves the SSD upward in the memory hierarchy, usable as a larger, slower form of RAM instead of just a cache for the hard drive. Using SSDAlloc, applications can nearly transparently extend their memory footprints to hundreds of gigabytes and beyond without restructuring, well beyond the RAM capacities of most servers. Additionally, SSDAlloc can extract 90% of the SSD's raw performance while increasing the lifetime of the SSD by up to 32 times. Other approaches either require intrusive application changes or deliver only 6- 30% of the SSD's raw performance.
Participation of Xenon Guest in Hydrogen Bond Network of β-Hydroquinone Crystal (2012)

In the β-hydroquinone (β-HQ)-Xe crystal, the Xe guest is placed between two hexagonal rings of coupled [···O-H···O-](6) H-bonds. This clathrate is treated as the model for monitoring the H-bonding system with the Xe participation. Three kinds of isotope effects due to the H/D substitution in the [···O-H···O-](6) bonds are considered: (i) structural changes in the clathrate (X-ray diffraction), (ii) variations of (129)Xe NMR signal of the guest (CP MAS), and (iii) variations of selected vibrations of the host (IR). This study predicts subtle inclination of every other hydroxyl group of the [···O-H···O-](6) rings into the Xe atom and formation of six Xe···H-O pairs in every cage, the frequency shift of the γOH mode due to these contacts, -ΔγOH(Xe···H) > 74 cm(-1), as well as the enthalpy formation, -ΔH(Xe···H) > 6-8 kJ mol(-1). Our IR results reveal a tendency of the Xe atom to form the H-bond-like network inside its cage and much weaker Xe···D-O interactions in the H/D substituted crystal. The (129)Xe NMR results do not reflect this kind of interactions due to averaging of the (129)Xe shielding phenomena, probably. We also predict elongation of the O···O distances due to the β-HQ-Xe crystal heating and the Xe escape.
Chemical Vapour Deposition Graphene–PMMA Nanolaminates for Flexible Gas Barrier (2022)

Successful ways of fully exploiting the excellent structural and multifunctional performance of graphene and related materials are of great scientific and technological interest. New opportunities are provided by the fabrication of a novel class of nanocomposites with a nanolaminate architecture. In this work, by using the iterative lift-off/float-on process combined with wet depositions, we incorporated cm-size graphene monolayers produced via Chemical Vapour Deposition into a poly (methyl methacrylate) (PMMA) matrix with a controlled, alternate-layered structure. The produced nanolaminate shows a significant improvement in mechanical properties, with enhanced stiffness, strength and toughness, with the addition of only 0.06 vol% of graphene. Furthermore, oxygen and carbon dioxide permeability measurements performed at different relative humidity levels, reveal that the addition of graphene leads to significant reduction of permeability, compared to neat PMMA. Overall, we demonstrate that the produced graphene-PMMA nanolaminate surpasses, in terms of gas barrier properties, the traditional discontinuous graphene-particle composites with a similar filler content. Moreover, we found that the gas permeability through the nanocomposites departs from a monotonic decrease as a function of relative humidity, which is instead evident in the case of the pure PMMA nanolaminate. This work suggests the possible use of Chemical Vapour Deposition graphene-polymer nanolaminates as a flexible gas barrier, thus enlarging the spectrum of applications for this novel material.
Shape classification of altimetric signals using anomaly detection and bayes decision rule (2010)

This paper addresses the problem of classifying altimetric signals according to their shapes. The proposed classifier is divided into three steps. A one-class support vector machine method is first used to isolate the large amount of Brown-like echoes from others signals which are considered as outliers. The second step extracts pertinent features from the the remaining echoes (which cannot be well described by the Brown model). These features are projected onto discriminant axes using linear discriminant analysis. The final step classifies the projected feature vectors using a standard Bayesian classifier. The proposed three step classification strategy is evaluated on supervised real altimetric echoes.
Reaction of a-Terpinene with Methylphosphonous Dichloride (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReaction of a-Terpinene with Methylphosphonous DichlorideLouis Quin, Kenneth Caster, and S SolowayCite this: J. Org. Chem. 1984, 49, 14, 2627–2629Publication Date (Print):July 1, 1984Publication History Published online30 December 2003Published inissue 1 July 1984https://pubs.acs.org/doi/10.1021/jo00188a600https://doi.org/10.1021/jo00188a600research-articleACS PublicationsRequest reuse permissionsArticle Views74Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effects of Acute Exercise on Anxiety Ratings in Patients With Coronary Heart Disease and Elevated Anxiety (2021)

Although many variables were examined, only higher baseline anxiety distinguished those who showed a reduction in their visual analog scale ratings from those whose anxiety remained the same or got worse. The extent to which the response to acute exercise predicts the anxiolytic effects of chronic exercise needs further study.
Preparation of LiNi_(0.80)Co_(0.15)Al_(0.05)O_2 cathode material via Li-rich method (2014)

Spherical Ni0.80Co0.15Al0.05(OH)2.05 precursors were synthesized via precipitation method. The Ni0.80Co0.15Al0.05(OH)2.05 precursors were mixed with excess lithium sources. LiNi0.80Co0.15Al0.05O2 cathode materials were obtained after sintering the mixtures at O2 and air atmosphere, respectively. The structure and morphology of the materials were characterized by X-ray diffraction(XRD) and scanning electron microscopy(SEM). The electrochemical performance of the cathode materials were analyzed by constant current charge-discharge test methods. When the molar ratio of Li :(Ni+Co+Al) is 1.15, the structure, morphology and electrochemical performance of the LiNi0.80Co0.15 Al0.05O2 cathode sample obtained at air atmosphere are very similar with those of the sample made at O2 atmosphere. It was proved that LiNi0.80Co0.15Al0.05O2 cathode materials with excellent performance can also be synthesized even at air atmosphere with the help of excess Li. At rate of 0.1 C and 1 C, the cathode materials showed initial capacity of 200 mA·h/g and 168 mA·h/g, respectively, and retention of more than 80% after 800 cycles.
The Interactions of Eco-Labeling, Environment, and International Trade (2000)

A simple graphical model has been developed to examine the relationship between eco-labelling, international trade and environment. This paper analyses that labelling can possibly have adverse effect on environment when the supply of environment-friendly good is greater than the demand of the friendly good pre-labelling (fig.2). In a dynamic setting, however, this situation could be reversed by shifts of demand and supply curves of environment-friendly products (fig.3). The theoretical model predicts change of product prices, which in turn can alter international trade. In bilateral trade, the interaction with country 2 will result in an improvement in the environmental situation in country 1 only if there is offsetting demand for the environment-friendly good from that country. The environmental situation in country 1 would worsen under international trade only if there is offsetting excess supply of the environment-friendly products from abroad. An empirical case of US tuna export is studied and “dolphin-safe” labelling is found to have a negative coefficient with tuna export volume from US to Canada.
O-48 - The international study to predict optimized treatment - in depression: rational, design and initial findings (2012)

The aim of iSPOT-D is to identify biological pretreatment measures that predict or moderate MDD treatment response or remission to escitalopram, sertraline or venlafaxine; and develop a model that incorporates multiple predictors and moderators. The iSPOT-D study is a multi-center, international, randomized, prospective, open-label trial (1). It is enrolling 2016 MDD outpatients (ages 18–65) from primary or specialty care practices (672 per treatment arm; 672 age-, sex- and education-matched healthy controls). Study-eligible patients are antidepressant medication (ADM) naïve or washed-out with no protocol ADM contraindications. Baseline assessments include symptoms; distress; daily function; cognitive performance; electroencephalogram and event-related potentials; heart rate and genetic measures. A subset of these baseline assessments are repeated after eight weeks of treatment. Outcomes include the 17-item Hamilton Rating Scale for Depression (primary) and self-reported depressive symptoms, social functioning, quality of life, emotional regulation, and side-effect burden (secondary). Participants may then enter a naturalistic telephone follow-up at weeks 12, 16, 24 and 52. The first half of the sample will be used to identify potential predictors and moderators, and the second half to replicate and confirm. First enrolment was in December 2008, and enrollment of the first 50% (1008 MDD participants) was completed in Dec 2010. iSPOT-D evaluates clinical and biological predictors of treatment response in the largest known sample of MDD collected worldwide. Initial findings reveal a remission rate of 45.4% and a response rate of 62.6% after 6–8 weeks of treatment. Initial findings will be discussed including factors for response prediction and MDD subtype differences.
Ultrastructural demonstration of CD36 in the alpha-granule membrane of human platelets and megakaryocytes (1993)

CD36 (glycoprotein [GP] IV) is a membrane GP of 88 kD found on monocytes, endothelial cells, and platelets. It may serve as a receptor for collagen and is also able to bind thrombospondin (TSP), because a monoclonal antibody to CD36 inhibits TSP binding to thrombin-stimulated platelets. In the following study, we investigated the subcellular distribution of CD36 within normal resting platelets, thrombin-stimulated platelets, and in cultured megakaryocytes (MK) by an immunogold staining technique and electron microscopy. We used an affinity-purified monospecific polyclonal antibody showing a single major band of precipitation at 88 kD via immunoblot analysis. In normal platelets, ultrastructural observation detected immunolabeling for CD36, homogeneously distributed along the platelet plasma membrane and in the luminal side of the open canalicular system (OCS). Moreover, some labeling was found around the alpha-granules along the inner face of their limiting membrane. An average of 70% of granules were labeled. The granule-associated pool of CD36 was estimated at approximately 25% of the total cell content. To exclude the possibility of a cross-reaction with GPIIb-IIIa, platelets from a patient with type I Glanzmann's thrombasthenia (which completely lack GPIIb-IIIa) were studied and showed a similar subcellular distribution of CD36, including alpha-granule membrane labeling. In activated platelets, CD36 was shown to be redistributed to the OCS and pseudopods of the plasma membrane. Platelets from a patient with the Gray platelet syndrome expressed CD36 on their plasma membrane, and some immunolabeling was also found within small abnormal alpha-granules. In cultured MK, CD36 immunolabeling was detected in the Golgi saccules, associated vesicles, immature alpha-granules, and demarcation membranes. In conclusion, this study shows the existence of a significant intragranular pool of CD36 in platelets that may play a critical role in the surface expression of alpha-granule TSP during platelet activation.
The Impact of Predilation Prior to Transcatheter Aortic Valve Implantation with the Self-Expanding ACURATE Neo Device: From the Multicenter Neopro Registry (2021)

Objectives: Transcatheter aortic valve replacement (TAVR) has become a first-line therapeutic option in patients with severe, symptomatic aortic stenosis with a high or intermediate surgical risk. The self-expanding ACURATE neo bioprosthesis (Boston Scientific, Marlborough, Massachusetts) is a next-generation THV that provided promising clinical and echocardiographic results. Considering the low radial force of the ACURATE neo THV, preimplantation balloon aortic valve valvuloplasty (pre-BAV) before valve implantation was recommended and almost always performed in early experience with this device. However, omitting predilatation could be appealing to simplify the procedure and reduce the risk of potential balloon aortic valvuloplasty (BAV)-related complications. Aim of this study was to evaluate the use and effects of pre-BAV prior to TAVR with ACURATE neo THV.
Centrally Acting Antiemetics Mitigate Nausea and Vomiting in Patients With Alzheimer's Disease Who Receive Rivastigmine (2002)

Jhee, Stanford S.; Shiovitz, Thomas; Hartman, Richard D.; Messina, John; Anand, Ravi; Sramek, John; Cutler, Neal R. Author Information
DNA Assembly in 3D Printed Fluidics (2015)

The process of connecting genetic parts-DNA assembly-is a foundational technology for synthetic biology. Microfluidics present an attractive solution for minimizing use of costly reagents, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication and operation can be expensive and requires expertise, limiting access to the technology. With advances in commodity digital fabrication tools, it is now possible to directly print fluidic devices and supporting hardware. 3D printed micro- and millifluidic devices are inexpensive, easy to make and quick to produce. We demonstrate Golden Gate DNA assembly in 3D-printed fluidics with reaction volumes as small as 490 nL, channel widths as fine as 220 microns, and per unit part costs ranging from $0.61 to $5.71. A 3D-printed syringe pump with an accompanying programmable software interface was designed and fabricated to operate the devices. Quick turnaround and inexpensive materials allowed for rapid exploration of device parameters, demonstrating a manufacturing paradigm for designing and fabricating hardware for synthetic biology.
A-ring modified betulinic acid derivatives as potent cancer preventive agents (2020)

Ten new 3,4-seco betulinic acid (BA) derivatives were designed and synthesized. Among them, compounds 7–15 exhibited enhanced chemopreventive ability in an in vitro short-term 12-O-tetradecanoylphorbol-13-acetate (TPA) induced Epstein-Barr virus early antigen (EBV-EA) activation assay in Raji cells. Specifically, analogs with a free C-28 carboxylic acid, including 7, 8, 11, and 13, inhibited EBV-EA activation significantly. The most potent compound 8 displayed 100% inhibition at 1 × 103 mol ratio/TPA and 73. 4%, 35. 9%, and 8. 4% inhibition at 5 × 102, 1 × 102, and 1 × 10 mol ratio/TPA, respectively, comparable with curcumin at high concentration and better than curcumin at low concentration. The potent chemopreventive activity of novel seco A-ring BAs (8 and 11) was further confirmed in an in vivo mouse skin carcinogenesis assay.
VIBRATIONAL CIRCULAR DICHROISM IN AMIDE STRETCHING VIBRATIONS FOR A VARIETY OF POLYPEPTIDES (1981)

$^{1}$J. Snir, R. A. Frankel and J. A. Schellman, and Biopolymers (1975), 14, 173. $^{2}R$. D. Singh and T. A. Keiderling, Biopolymers (1981), 20, 237.
Approaching the Treatment of AML from a Biological Perspective (2007)

Current acute myeloid leukemia (AML) therapy is inadequate for the great majority of patients. Major challenges are peculiar to the elderly and the infirm. There is no prospect of further advances using current standard agents. At our current state of knowledge, all potential classes of agents merit clinical investigation. However, the empirical approach can certainly be refined by the application of recent data on the pathophysiology of AML, its molecular biology, the aberrations that drive the proliferative and antiapoptotic leukemia advantages, and the study of resistance to standard and novel agents. In this chapter we review some of the more promising novel agents in AML.