gebhart/patrepeval huggingface dataset:
NK cell peak after seizures (2007)

Local and systemic immunological alterations may affect the course of focal epilepsy. However, little is known about the influence of seizures on the cellular immunity which we analyzed prospectively in epilepsy patients during video EEG monitoring.
The behavior of a magnetorheological (MR) fluid under compressive deformation (2015)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation William Kordonski, Sergei Gorodkin; The behavior of a magnetorheological (MR) fluid under compressive deformation. J. Rheol. 1 January 2016; 60 (1): 129–139. https://doi.org/10.1122/1.4938049 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentThe Society of RheologyJournal of Rheology Search Advanced Search |Citation Search
Toward real-time measurement of atmospheric mercury concentrations using cavity ring-down spectroscopy (2010)

Abstract. Cavity ring-down spectroscopy (CRDS) is a direct absorption technique that utilizes path lengths up to multiple kilometers in a compact absorption cell and has a significantly higher sensitivity than conventional absorption spectroscopy. This tool opens new prospects for study of gaseous elemental mercury (Hg0) because of its high temporal resolution and reduced sample volume requirements (&lt;0.5 l of sample air). We developed a new sensor based on CRDS for measurement of (Hg0) mass concentration. Sensor characteristics include sub-ng m−3 detection limit and high temporal resolution using a frequency-doubled, tuneable dye laser emitting pulses at ~253.65 nm with a pulse repetition frequency of 50 Hz. The dye laser incorporates a unique piezo element attached to its tuning grating allowing it to tune the laser on and off the Hg0 absorption line on a pulse-to-pulse basis to facilitate differential absorption measurements. Hg0 absorption measurements with this CRDS laboratory prototype are highly linearly related to Hg0 concentrations determined by a Tekran 2537B analyzer over an Hg0 concentration range from 0.2 ng m−3 to 573 ng m−3, implying excellent linearity of both instruments. The current CRDS instrument has a sensitivity of 0.10 ng Hg0 m−3 at 10-s time resolution. Ambient-air tests showed that background Hg0 levels can be detected at low temporal resolution (i.e., 1 s), but also highlight a need for high-frequency (i.e., pulse-to-pulse) differential on/off-line tuning of the laser wavelength to account for instabilities of the CRDS system and variable background absorption interferences. Future applications may include ambient Hg0 flux measurements with eddy covariance techniques, which require measurements of Hg0 concentrations with sub-ng m−3 sensitivity and sub-second time resolution.
Directing spatial disposition of ferrocene around homoadenine tetrads (2008)

We report synthesis and crystallographic studies of a ferrocenyl conjugate of adenine, where the hydrogen bonding interactions promote and stabilize nucleobase homotetrad formation.
HSM-based Key Management Solution for Ethereum Blockchain (2021)

The security of distributed applications backed by blockchain technology relies mainly on keeping the associated cryptographic keys (i.e. private keys) in well-protected storage. Since they are the unique proof of ownership of the underlying digital assets. If the keys are stolen or lost, there is no way to recover the assets. The cold wallet is a good candidate for basic use cases, but it has a substantial challenge for more complex applications as it does not scale. Warm and hot wallets are more convenient options for blockchain-based solutions that aim to transact in a cloud environment. In this work, we focus on Hardware Security Module (HSM) based wallet. The HSM is the de-facto standard device designed to manage high-value cryptographic keys and to protect them against hacks. In this demonstration, we present an HSM-based working prototype that secures the entire life cycle of Ethereum public and private keys.
Adaptive versus non-adaptive strategies for quantum channel discrimination (2009)

We provide a simple example that illustrates the advantage of adaptive over non-adaptive strategies for quantum channel discrimination. In particular, we give a pair of entanglement-breaking channels that can be perfectly discriminated by means of an adaptive strategy that requires just two channel evaluations, but for which no non-adaptive strategy can give a perfect discrimination using any finite number of channel evaluations.
Supplementary Figures S1-3 and Table S1 from 23814, an Inhibitory Antibody of Ligand-Mediated Notch1 Activation, Modulates Angiogenesis and Inhibits Tumor Growth without Gastrointestinal Toxicity (2023)

&lt;p&gt;Supplementary Figures S1-3 and Table S1. Supplementary Figure S1: Histopathology and 23814 Serum Concentration at end of toxicity study Supplementary Figure S2: 23814 Treatment Increases Vasculature Even in Tumors with Limited Response Supplementary Figure S3: 23814/Tivozanib Combination Treatment Increases Tumor Necrosis Supplementary Table S1: Clinical Pathology Analysis&lt;/p&gt;
High-performance resistive switching characteristics of programmable metallization cell with oxidized Cu-Ti electrodes (2013)

Programmable metallization cell (PMC) memory devices with oxidized Cu-Ti alloy films as the bottom electrodes have been shown to exhibit a superior on/off state current ratio (memory window) of as high as 103 and endurance of 3000 cycles as compared to conventional pure copper and unoxidized Cu-Ti alloy electrodes. It was conjectured that the Cu-Ti alloy electrodes could obtain the appropriate amount of copper atoms to format and rupture the conductive filaments in the resistive switching layer. Furthermore, the oxidized Cu-Ti alloys could control the Cu cations from the Cu and Cu2O to the appropriate amountto achieve the most favorable PMC characteristics.
A 56-Gb/s Transimpedance Amplifier in 0.13-µm SiGe BiCMOS for an Optical Receiver with -18.8-dBm Input Sensitivity (2016)

This paper describes a 56-Gb/s transimpedance amplifier with a level-shift circuit and double-feedback-loop (DFB) compensation architecture to achieve high input sensitivity. The level-shift circuit placed between a main transimpedance amplifier and a post amplifier mitigates the trade-off between the bandwidth and noise of the receiver, which reduces the input referred noise by 70%. The DFB compensates for process variation without increasing the noise. The transimpedance amplifier was fabricated in 0.13-um SiGe BiCMOS technology and packaged with an avalanche photodiode. The 3-dB bandwidth of 38.4 GHz and the input referred noise current density of 14.8 pA/rtHz are achieved. These are the best performance among published 50-Gb/s-class transimpedance amplifiers for optical communication.
5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO): a new phosphorylated nitrone for the efficient In Vitro and In Vivo spin trapping of oxygen-centred radicals (1994)

5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), a newly synthesized spin trap, is used for the in vitro spin trapping of hydroxyl and superoxide radicals, and though the spin trapping rates are close to those reported for 5,5-dimethyl-1-pyrroline N-oxide (DMPO), the DEPMPO–superoxide spin adduct is significantly more persistent than its DMPO analogue, a difference which allows the detection of superoxide during the reperfusion of ischaemic isolated rat hearts.
Cell Engineering with Synthetic Messenger RNA (2012)

mRNA has become an important alternative to DNA as a tool for cell reprogramming. To be expressed, exogenous DNA must be transmitted through the cell cytoplasm and placed into the nucleus. In contrast, exogenous mRNA simply has to be delivered into the cytoplasm. This can result in a highly uniform transfection of the whole population of cells, an advantage that has not been observed with DNA transfer. The use of mRNA, instead of DNA, in medical applications increases protocol safety by abolishing the risk of transgene insertion into host genomes. In this chapter, we review the aspects of mRNA structure and function that are important for its "transgenic" behavior, such as the composition of mRNA molecules and complexes with RNA binding proteins, localization of mRNA in cytoplasmic compartments, translation, and the duration of mRNA expression. In immunotherapy, mRNA is employed in reprogramming of antigen presenting cells (vaccination) and cytolytic lymphocytes. Other applications include generation of induced pluripotent stem (iPS) cells, and genome engineering with modularly assembled nucleases. The most investigated applications of mRNA technology are also reviewed here.
Accelerated tumor growth in mice deficient in DNAM-1 receptor (2008)

Since the identification of ligands for human and mouse DNAM-1, emerging evidence has suggested that DNAM-1 plays an important role in the T cell- and natural killer (NK) cell-mediated recognition and lysis of tumor cells. However, it remains undetermined whether DNAM-1 is involved in tumor immune surveillance in vivo. We addressed this question by using DNAM-1-deficient mice. DNAM-1-deficient cytotoxic T lymphocyte (CTL) and NK cells showed significantly less cytotoxic activity against DNAM-1 ligand-expressing tumors in vitro than wild-type (WT) cells. The methylcholanthrene (MCA)-induced fibrosarcoma cell line Meth A expressed the DNAM-1 ligand CD155, and DNAM-1-deficient mice showed increased tumor development and mortality after transplantation of Meth A cells. Moreover, the DNAM-1-deficient mice developed significantly more DNAM-1 ligand-expressing fibrosarcoma and papilloma cells in response to the chemical carcinogens MCA and 7,12-dimethylbenz[a]anthracene (DMBA), respectively, than did WT mice. These results indicate that DNAM-1 plays an important role in immune surveillance of tumor development.
TNFR25 Expression on CD4+CD25+ T Cells: Down Modulation of Regulatory Activity. (2006)

Abstract TNFR25 (“DR3”) is a member of the TNF receptor family that is expressed by activated CD4+ and CD8+ T cells. To determine if activated CD4+CD25+ T cells also expressed this TNFR family molecule, B6 CD4+CD25+ T cells were stimulated with anti-CD3/CD28 coated beads (kind gift of Dr. B. Blazar, U. Minn.) and expanded for 3–4 days. TNFR25 expression was readily detected on CD4+CD25+ FoxP3+ T cells. Since other members of the TNF receptor family (GITR, OX40, 4–1BB) are known to influence T regulatory cell function, we investigated whether TNFR25 signaling can regulate CD4+CD25+ T cell activity. TNFR25 triggering in B6-wt T regulatory CD4+CD25+ cells with the recombinant TNFR25 ligand TL1A or agonistic anti-TNFR25 antibody (4C12) resulted in reduction of their ability to suppress anti-CD3 induced ex-vivo proliferation of CD4+CD25− cells. 4C12 mediated TNFR25 signaling also reduced B6-wt Treg mediated inhibition of peptide induced proliferation of OVA-specific B6 CD8+ (OT-I) cells. To further investigate a role for TNFR25 in Treg cell regulation, TNFR25 (full length) transgenic mice were generated and bred onto the BL/6 background. CD4+CD25+ cells from these TNFR25 tg mice were found to possess diminished T regulatory activity in vitro as determined by their diminished inability to regulate proliferation by B6-wt CD4+ and OT-I CD8+ T cells. To assess their in vivo regulatory activity, B6-wt and B6 TNFR25 tg Treg cells were examined for their ability to inhibit graft vs. host disease (GVHD) following allogeneic MHC class I/II mismatched BMT. In contrast to B6-wt Treg cells, TNFR25 tg Treg cells exhibited significantly diminished ability to regulate the onset of GVHD in vivo as assessed by weight loss and clinical symptoms. Using agonistic antibody, stimulation of TNFR25 on transgenic Treg cells was also found to effectively remove the ex-vivo regulatory activity expressed by this population. To exclude any possible direct co-stimulatory effects of 4C12 antibody on the responding proliferating cells, CD4+CD25−T cells from TNFR25 dominant negative transgenic mice were employed. 4C12 mab again abolished Treg cell inhibitory activity. The effect of TNFR25 agonists on T reg cell activity in vivo is being further investigated in both mouse models of GVHD and IBD diseases. Initial observations administering 4C12 post-allogeneic BMT together with B6-wt Treg cells indicate a reduction in their ability to regulate GVHD. In total, these studies identify TNFR25 as a new potential target for augmenting CD4+ and CD8+ responses by concomitant direct co-stimulation of effecter cells and inhibition of T regulatory cell function.
Local Editing of Procedural Models (2019)

Abstract Procedural modeling is used across many industries for rapid 3D content creation. However, professional procedural tools often lack artistic control, requiring manual edits on baked results, diminishing the advantages of a procedural modeling pipeline. Previous approaches to enable local artistic control require special annotations of the procedural system and manual exploration of potential edit locations. Therefore, we propose a novel approach to discover meaningful and non‐redundant good edit locations (GELs). We introduce a bottom‐up algorithm for finding GELs directly from the attributes in procedural models, without special annotations. To make attribute edits at GELs persistent, we analyze their local spatial context and construct a meta‐locator to uniquely specify their structure. Meta‐locators are calculated independently per attribute, making them robust against changes in the procedural system. Functions on meta‐locators enable intuitive and robust multi‐selections. Finally, we introduce an algorithm to transfer meta‐locators to a different procedural model. We show that our approach greatly simplifies the exploration of the local edit space, and we demonstrate its usefulness in a user study and multiple real‐world examples.
Superficially Porous Particle Based Hydroxypropyl‐β‐cyclodextrin Stationary Phase for High‐Efficiency Enantiomeric Separations (2015)

A superficially porous particle (SPP)-based hydroxypropyl-β-cyclodextrin (HPBCD) chiral stationary phase (CSP) was produced and its chromatographic performance was compared to both 5 µm and 3 µm fully porous particle (FPP)-based CSPs. The relative surface coverage of the HPBCD chiral selector on each particle was approximately equal, which resulted in equivalent enantiomeric selectivity (α) values on each phase when constant mobile phase conditions were used. Under such conditions, the SPP column resulted in greatly reduced analysis times and three times greater efficiencies compared to the FPP columns. When higher flow rates were used, efficiency gains per analysis times were five times greater for the SPP column compared to the FPP-based columns. When the mobile phases were altered to give similar analysis times on each column, resolution values were doubled for the SPP column. Finally, the novel SPP based HPBCD column proved to be stable for 500 injections under high flow rate (4.5 mL/min) and high pressure (400 bar) conditions used for an ultrafast (~45 sec) enantiomeric separation.
Isolating and Culturing of Precursor Cells from the Adult Human Brain (2013)

Adult neural precursor cells are an essential part of the brain, and a focus of two decades of intense research (Ming and Song, Neuron 70:687-702, 2011). Even though adult human stem/progenitor cells have been identified early on (Kirschenbaum et al., Cereb Cortex 4:576-589, 1994; Eriksson et al., Nat Med 4:1313-1317, 1998), progress in the field of adult human neurogenesis has been slow. The reasons for this may be more advanced neighboring fields of pluripotent stem cell research, and lacking study material as well as well-established and standardized protocols. Furthermore, adult precursor cells in humans seem to have greater potential than in rodents (Walton et al., Development 133:3671-3681, 2006). This may be attributed to species differences in astrocyte development and diversity (Oberheim et al., Neurosci 29:3276-3287, 2009). In this chapter, we provide a guideline for adult human brain tissue dissociation, be it from biopsy or autopsy specimens. This is by no means the only way of culturing adult neural precursor cells, but it may help in streamlining research on this fascinating topic, as well as help introducing others into this field. We describe our methodology for establishing and maintaining long-term cultures from white and grey matter, as well as a simple protocol for differentiating these cells.
Diminished Band-Gap Transitions of Single-Walled Carbon Nanotubes in Complexation with Aromatic Molecules (2004)

The near-IR absorption spectrum of semiconducting single-walled carbon nanotubes is characterized by the transitions associated with the first (S11) and second (S22) pairs of van Hove singularities in the electronic density of states. We report that a significant effect on the transitions can be caused by non-covalent complexation of the nanotube with planar aromatic molecules such as pyrenes in solution, resulting in the absence of S11 and S22 bands in the near-IR absorption spectrum. Since the complexation is reversible, the characteristic absorption bands can be turned on and off with the complexation in a reversible fashion.
Most free‐radical injury is iron‐related: It is promoted by iron, hemin, holoferritin and vitamin c, and inhibited by desferoxamine and apoferritin (1994)

Iron is a double-edged sword. In moderate quantities and leashed to protein, it is an essential element in all cell metabolism and growth, but it is toxic when unleashed. Because of its ability to switch back and forth between ferrous and ferric oxidation states, iron is both a strong biological oxidant and reductant. The human diet contains a multitude of natural chemicals which are carcinogens and anticarcinogens, many of which act by generating oxygen radicals, which initiate degenerative processes related to cancer, heart disease and aging (the "oxygen radical hypothesis of aging"). Among these many dietary chemicals are many redox agents, including vitamin C and beta carotene. Free radical damage is produced primarily by the hydroxyl radical (.OH). Most of the .OH generated in vivo comes from iron-dependent reduction of H2O2. Supporting too much iron as a free radical-generating culprit in the risk of cancer, NHANES I data indicated that high body iron stores, manifested by increased transferrin saturation, are associated with an increased cancer risk. Other data shows an increased heart attack risk.
New Strategy for the Construction of Single-Stranded Plasmids with Single Mutagenic Lesions (1997)

Single-stranded DNA vectors containing single adducts offer a unique opportunity to study the biochemistry and genetics of trans lesion synthesis, a process during which a DNA polymerase synthesizes across a lesion. We describe a new and general strategy to produce high-quality single-stranded plasmids containing a single adduct within a predetermined sequence context starting with a short oligonucleotide containing the lesion of interest. These vectors are isolated from the corresponding double-stranded constructs by selective enzymatic degradation in vitro of the nonadducted uracil-containing strand. Efficient and complete removal of this strand was achieved using uracil DNA glycosilase to generate AP sites followed by the action of the AP endonuclease associated with exonuclease III and the robust 3'-->5' exonuclease activity associated with T7 DNA polymerase. We show the utility of these constructs for the study of trans lesion synthesis in vitro and in vivo in the case of the highly carcinogenic N-2-acetylaminofluorene adducts located within frameshift mutation hot spots. The possibility to construct both single-stranded and double-stranded plasmids, with the same origin of replication (i.e., ColE1), will allow a direct comparison between single-stranded and double-stranded DNA replication in site-specific mutagenesis studies.
SCATTERING PARAMETER TRANSIENT ANALYSIS OF INTERCONNECT NETWORKS WITH NONLINEAR TERMINATIONS USING RECURSIVE CONVOLUTION (1993)

A novel method for analyzing interconnect networks with nonlinear terminations is presented. The circuit is partitioned into linear and nonlinear networks. A scattering parameter based macromodel is introduced to model the linear network. An efficient network reduction algorithm is developed to reduce the linear network into a network containing one multiport component (macromodel) together with sources and loads of interest. Exponentially Decayed Polynomial Functions(EDPF) are used to approximate the scattering parameters of the macromodel, which is always stable for stable circuits. In order to incorporate the macromodel into a SPICE like circuit simulator, a simplified recursive convolution formula is developed and Norton equivalent circuits are derived based on recursive convolution. Experiment results indicate that our method can approach the accuracy of SPICE3e2 with order of magnitudes less computing time. Keywords: scattering parameter, macromodel, multiport component, multipor...
Excited State Dynamics of the Third Order Nonlinear Optical Susceptibility in Conjugated Linear Chains (1993)

We have earlier presented theoretical and experimental results demonstrating that the nonresonant third order optical susceptibility χ (3) (−ω;ω,ω,−ω) can be enhanced for nonlinear optical processes originating from real population of electronic excited states in conjugated linear chains[1,2]. Compared with the ground state, χ (3) (−ω;ω,ω,−ω) of π-conjugated linear chains can be enhanced by orders of magnitude when the first S 1 or second S 2 π-electron excited state is optically pumped and then populated for times long enough to perform nonresonant measurements of χ (3) (−ω;ω,ω,−ω) at frequencies different from the resonant pump frequency. In this paper, a dynamical model is developed for the time evolution of the nonresonant microscoopic degenerate four wave mixing (DFWM) susceptibility γ(-ω;ω,ω,-ω) for the excited state enhancement mechanism. The model is applied to the case of the six site chain, diphenylhexatriene (DPH).
Coke Formation in the Combustion of Isolated Heavy Oil Droplets (1984)

Abstract The combustion and coking of isolated, free and suspended droplets of a residual oil have been studied. The combustion history of the free and suspended droplets was found to be characterized by qualitatively similar phenomena. The coke particle was seen to form within the final 9 percent of the droplet burning period and the mass of the coke particle was about 3 percent of the mass of the initial residual oil droplet. Changing the temperature history of a droplet by varying the initial droplet size between 260 and 570 microns or by diluting the residual oil with a more volatile No. 2 fuel oil did not affect the mass fraction of the residual oil in the initial droplet which was converted to coke. The contribution to the coke particle from both the asphaltene and maltenc (residual oil from which the asphaltenes have been removed) components of the oil was found to be significant, and therefore fuel asphaltene content alone will not provide a satisfactory basis for determining the relative coking tendency of different oils. The results indicate that in the combustion of heavy fuel oils coke particles will be formed and that particulate emissions may be best reduced through enahanced particle burnout (through techniques such as improved atomization and oil-water emulsions), and not through an attempted reduction in the formation of coke.
Dibenzoannulated 3,5,3″,5″-Tetra(t-butyl)-p-terphenoquinone. A Reversible, Photochemical-Thermal Switching System Involving Restricted Conformational Change (1999)

Abstract The title compound, a new terphenoquinone derivative, shows a dynamic redox behavior upon cyclic voltammetry and a reversible, photochemical-thermal isomerization between the quinone and diradical forms, both phenomena involving restricted conformational change.