gebhart/patrepeval huggingface dataset:
26: Softwaretechnik 1, Vorlesung, SS 2017, 28.07.2017 (2017)

26 | 0:00:00 Starten 0:00:08 Qualitätsschätzung 0:00:49 Das ""Teufelsquadrat"" 0:01:37 Einflussfaktoren 0:01:41 Wie kommt man auf geschätzte LOC oder PM? 0:02:27 Analogiemethode: Beispiel 0:05:30 Die Schätzmethode COCOMO II 0:08:20 COCOMO II Skalierungsfaktoren 0:10:05 COCOMO II Multiplikative Kostenfaktoren 0:10:53 COCOMO II - Beispielrechnung 0:17:39 Prozessmodelle 0:18:48 Programmieren durch Probieren 0:21:02 Wasserfallmodell 0:25:08 V-Modell 97 0:26:18 V-Modell XT 0:29:55 Prototypmodell 0:32:19 Iteratives Modell 0:35:51 Synchronisiere und Stabilisiere 0:46:44 Agiles Manifest 0:48:02 Agile Prozesse 0:50:28 Praktiken des Extreme Programming (XP) 0:55:09 Paarprogramierung 1:00:52 Effizientes Testen 1:01:23 Testen bei XP 1:01:48 Testgetriebene Entwicklung 1:05:12 Test / Implementierung / Umstrukturierung 1:07:31 Inkrementelles Design 1:12:14 Behauptung Kent Becks 1:13:11 Kritik an XP 1:13:51 Probleme bei der Einführung von XP 1:16:03 Bewertung von XP 1:17:50 Zusammenfassung 1:19:57 Scrum 1:22:29 Restaufwands-Diagramm 1:24:29 Scrum - Rollen 1:25:03 Scrum-Treffen
Evaluating crime prevention: Lessons from large-scale community crime prevention programs (2013)

The Australian Institute of Criminology has spent a number of years working with crime prevention agencies across Australia reviewing large-scale programs that involve the delivery of varying activities directed at the prevention of crime. Taken as a whole, this experience has shown that, despite good intentions and aspirations to evidence-based practice, both the level and quality of evaluations have been limited by several practical challenges. In turn, this has hampered efforts to develop a body of good quality Australian evidence about what is effective in preventing crime and what is required in order to deliver effective interventions. Using previously unpublished data collected as part of the reviews of two national Australian crime prevention programs, the authors examine the practical factors that impact on evaluation and make a number of important recommendations for the evaluation of projects delivered as part of large-scale community crime prevention programs. The authors argue that rather than persisting with traditional approaches that encourage local organisations to undertake potentially expensive and time-consuming evaluations of their own work, program managers and central agencies must become more proactive and increasingly innovative in their approaches to evaluation.
Abstract 90: Mining for cancer causing mutations in whole genome sequence data (2010)

Abstract The Cancer Genome Project at the Wellcome Trust Sanger Institute, UK is using massively parallel sequencing technologies to systematically screen exome and genome sequence for diverse categories of mutations. The rapid developments in new sequencing technologies have allowed many new approaches for the detection and characterisation of a wide range of mutation types. We employ a variety of automated and semi-automated techniques to identify and classify copy number variation, chromosomal rearrangements, small insertion/deletion and substitution mutations in both cell lines and primary tumours. Large scale, genome wide changes are detected by mapping read-pairs to a reference genome. Copy number is assessed by taking correctly mapping read-pairs, applying a circular binary segmentation algorithm and correcting for regional mapping bias throughout the genome. Using the high quality read mappings where the paired reads are an unexpected distance apart, putative rearrangements can be identified and confirmed either by local de novo sequence assembly where high sequencing coverage is available or by capillary sequencing for low coverage data. Smaller scale mutations are detected using local read coverage to provide detailed information on the genotype and allelic content of samples. The detection techniques can be used on both whole genome and exome pull-down sequence data. Substitutions are detected using a novel algorithm based on expectation maximisation (Calculate Variants with Expectation Maximisation using New sequencing technology, or CaVEMaN) written in-house and confirmed using capillary sequencing. Putative mutations of all types are compared to external resources (eg Ensembl, dbSNP) to infer genomic context and possible deleterious consequences. In-house tools have been developed to help automate the curation and annotation of mutations and once confirmed they are submitted to COSMIC as gene associated mutations, small scale intragenic mutations, chromosomal rearrangements or fusion gene products. Reference URL's Wellcome Trust Sanger Institute - http://www.sanger.ac.uk COSMIC - http://www.sanger.ac.uk/genetics/CGP/cosmic CGP - http://www.sanger.ac.uk/genetics/CGP/ Ensembl - http://www.ensembl.org dbSNP - http://www.ncbi.nlm.nih.gov/projects/SNP Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 90.
Adaptive parallax control for multi-view stereo panoramas (2006)

We describe techniques for stereo panoramic image capture and rendering that are part of a personal panoramic virtual environment system. We examine the use of stereo shutter-glasses and several recently developed autostereoscopic (AS) displays to improve the sense of immersion. The stereo panorama pair is created by stitching strips that are sampled from images captured with swing camera panoramic imaging system. We apply Peleg's disparity adjustment algorithm to the generated stereo panorama to achieve large disparity (horizontal parallax) of far away scenes and smaller disparity of closer scenes for stereo perception. Unfortunately, vertical parallax effects in the stereo panorama still occur, causing display artifacts and problems in human stereo fusion. To overcome these problems, we first present a general image capture model, specify geometrical parameters, and describe the panorama generating process. We then describe an efficient stitching algorithm that corrects dynamic exposure variation and removes moving objects without manual selection of ground-truth images. We present expressions for the horizontal and vertical parallax, describe parallax measurement techniques, and develop an adaptive vertical and horizontal parallax control algorithm for rendering in different viewing directions. We present a simple subjective test of stereo panoramas rendered on AS and other stereo displays, and discuss the relative quality of each.
Generation of Quorum Quenching Antibodies (2010)

The exchange of information within and among bacterial populations using small diffusible molecules has been termed "quorum sensing" (QS). Due to the extracellular distribution of the QS autoinducer molecules and the evolutionary highly conserved nature of signaling components, microbial QS systems represent an excellent target for anti-infective immunotherapy. Recently, we have described the generation of quorum quenching monoclonal antibodies (mAbs) against acyl homoserine lactones (AHL) used by Pseudomonas aeruginosa as well as Staphylococcal autoinducing peptides (AIP). These mAbs suppressed QS signaling in bacteria and neutralized AHL-mediated cytotoxic effects in vitro, as well as protected animals in Staphylococcus aureus infection models.
Glaciochemical Dating Techniques (1982)

Ice cores from Greenland and Antarctica contain valuable paleoenvironmental information from the past 100,000 years or more. Chemical analyses of the impurity content of polar snow and ice strata have proven useful in establishing and refining ice core chronologies through the identification of seasonal variations in concentrations and concentration event horizons. Measured concentration levels in polar precipitation range from about <1 ng/g to 50 ng/g necessitating the use of special contamination control procedures and analytical techniques such as INAA, IDMS, AAS, and ion chromatography [10]1. The identification of an annual spring maximum in concentrations of crustallyderived Al, Ca, and Fe, of an annual winter maximum in concentrations of marine-derived Na, Mg, and Cl, and of a summer maximum for other species each allow ice core dating by counting annual layers. These, and other ice core chronologies can be improved by comparing ages with the known occurrence of event horizons. Sulfuric acid derived from the 1783 Laki, Iceland eruption resulted in an acid snow layer with a SO42- concentration of 2200 ng/g which is more than an order of magnitude higher than in modern Greenland snow. The 1883 Krakatoa eruption resulted in Greenland concentrations of Cd, Zn, Pb, and Cu as much as a factor of 20 greater than average. Increased atmospheric turbidity during the last ice age resulted in prehistoric event horizons containing an order of magnitude more continental dust than in more recent ice.
Characterization of an Alginate Lyase, FlAlyA, from Flavobacterium sp. Strain UMI-01 and Its Expression in Escherichia coli (2014)

A major alginate lyase, FlAlyA, was purified from the periplasmic fraction of an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. FlAlyA showed a single band of ~30 kDa on SDS-PAGE and exhibited the optimal temperature and pH at 55 °C and pH 7.7, respectively. Analyses for substrate preference and reaction products indicated that FlAlyA was an endolytic poly(mannuronate) lyase (EC 4.2.2.3). A gene fragment encoding the amino-acid sequence of 288 residues for FlAlyA was amplified by inverse PCR. The N-terminal region of 21 residues except for the initiation Met in the deduced sequence was predicted as the signal peptide and the following region of six residues was regarded as propeptide, while the C-terminal region of 260 residues was regarded as the polysaccharide-lyase-family-7-type catalytic domain. The entire coding region for FlAlyA was subjected to the pCold I-Escherichia coli BL21(DE3) expression system and ~eight times higher yield of recombinant FlAlyA (recFlAlyA) than that of native FlAlyA was achieved. The recFlAlyA recovered in the periplasmic fraction of E. coli had lost the signal peptide region along with the N-terminal 3 residues of propeptide region. This suggested that the signal peptide of FlAlyA could function in part in E. coli.
Using IR Optical Marker Based Motion Capture for Exploring Musical Interaction (2010)

The paper presents a conceptual overview of how optical infrared marker based motion capture systems (IrMoCap) can be used in musical interaction. First we present a review of related work of using IrMoCap for musical control. This is followed by a discussion of possible features which can be exploited. Finally, the question of mapping movement features to sound features is presented and discussed.
ScRNA-seq expression of IFI27 and APOC2 identifies four alveolar macrophage superclusters in healthy BALF (2022)

Alveolar macrophages (AMs) reside on the luminal surface of the airways and alveoli, ensuring proper gas exchange by ingesting cellular debris and pathogens, and regulating inflammatory responses. Therefore, understanding the heterogeneity and diverse roles played by AMs, interstitial macrophages, and recruited monocytes is critical for treating airway diseases. We performed single-cell RNA sequencing on 113,213 bronchoalveolar lavage cells from four healthy and three uninflamed cystic fibrosis subjects and identified two MARCKS<sup>+</sup>LGMN<sup>+</sup>IMs, FOLR2<sup>+</sup>SELENOP<sup>+</sup> and SPP1<sup>+</sup>PLA2G7<sup>+</sup> IMs, monocyte subtypes, DC1, DC2, migDCs, plasmacytoid DCs, lymphocytes, epithelial cells, and four AM superclusters (families) based on the gene expression of <i>IFI27</i> and <i>APOC2</i> These four AM families have at least eight distinct functional members (subclusters) named after their differentially expressed gene(s): IGF1, CCL18, CXCL5, cholesterol, chemokine, metallothionein, interferon, and small-cluster AMs. Interestingly, the chemokine cluster further divides with each subcluster selectively expressing a unique combination of chemokines. One of the most striking observations, besides the heterogeneity, is the conservation of AM family members in relatively equal ratio across all AM superclusters and individuals. Transcriptional data and TotalSeq technology were used to investigate cell surface markers that distinguish resident AMs from recruited monocytes. Last, other AM datasets were projected onto our dataset. Similar AM superclusters and functional subclusters were observed, along with a significant increase in chemokine and IFN AM subclusters in individuals infected with COVID-19. Overall, functional specializations of the AM subclusters suggest that there are highly regulated AM niches with defined programming states, highlighting a clear division of labor.
MR 714, a new aryloxypropionic non-steroidal antiinflammatory agent. Chemical and pharmacokinetic properties and preliminary pharmacotoxicological data. (1983)

2-(4-(2',4'-Difluorophenyl)-phenoxy)propionic acid (MR 714) is endowed with an interesting analgesic and antiinflammatory activity, while any remarkable gastro-ulcerogenic action is virtually absent. From a toxicological point of view, MR 714 orally administered to mice showed a LD50 of 848 mg/kg; an observation of general behaviour proved that the drug is free of any remarkable effect on general behaviour, food and water intake, or diuresis after single or repeated administration. An autoptic examination after single oral doses (25, 50, 100 and 200 mg/kg) or repeated oral doses (100 mg/kg/day over 5 days) did not show any adverse modification and also the Ames test didn't allow any mutagenic action to be detected. From a pharmacokinetic point of view, MR 714 given p.o. to rats was absorbed excellently and plasma levels showed a peak after 4-6 h, followed by a plateau lasting 24-36 h at a dose of 50 or 100 mg/kg and considerably less at a dosage of 25 mg/kg. This lower dose allowed a dominant half-life (t1/2) of plasma levels of 13 h to be determined. Excretion occurred only via the bile in conjugated form (acylglucuronide) which reverted easily into the parent drug. The resultant enterohepatic circulation contributes to a substantial maintenance of plasma levels and hence long-lasting activity. Among the organs examined, the uterus showed the highest ratio of organ/plasma concentration; this evidence points to a possible clinical application of MR 714 in the treatment of dysmenorrhoea. From a chemical point of view, the aryloxypropionic structure may be regarded as an interesting novelty in the field of structure-activity relationship studies on non-steroidal antiinflammatory agents.
N-Glycosylation is requisite for the enzyme activity and Golgi retention of N-acetylglucosaminyltransferase III (1997)

UDP-N-acetylglucosamine: beta-D-mannoside beta-1,4N-acetylglucosaminyltransferase III (GnT-III, EC 2.4.1.144) is a glycoprotein involved in the biosynthesis of N-linked oligosaccharides. Rat GnT-III contains three potential N-glycosylation sites, which have been predicted to be Asn243, Asn261, and Asn399. To study the roles of N-glycosylation in the GnT-III function, rat GnT-III was expressed in COS-1 cells under tunicamycin or castanospermine treatment. The tunicamycin-treated GnT-III, which was not N-glycosylated, had almost no activity. The castanospermine-treated GnT-III was not localized in the Golgi, but glucosylation did not affect its activity. To clarify the role of individual N-glycosylations, we obtained a series of mutant cDNAs in which some or all of the potential glycosylation sites were eliminated by site-directed mutagenesis, and expressed them in COS-1 cells. All the mutants exhibited lower enzyme activity than the wild-type, but deglycosylation at individual sites had different effects on the enzyme activity. The deglycosylation at Asn243 or Asn261 was more effective on the activity than that at Asn399. The enzyme activity decreased as the number of glycosylation sites decreased. The null glycosylation mutant had no activity, corresponding to the case of tunicamycin-treated wild-type GnT-III. Kinetic analysis revealed that the deglycosylation at Asn243 or Asn261 resulted in slightly lower affinity for the donor substrate, but the other mutation did not significantly change the K(m) value for either the donor or acceptor. None of the mutant GnT-IIIs showed perinuclear localization or Golgi retention, that was observed for the wild-type protein. This is the first demonstration that the glycosyltransferase localized in the Golgi apparatus requires N-glycosylation for its activity and retention.
Superoxide dismutase and catalase in Frankia (1986)

Superoxide dismutase (SOD) and catalase (CAT) are important enzymes in the protection of cell components from oxidation via superoxide free radicals [Formula: see text] and hydrogen peroxide (H 2 O 2 ). SOD and CAT activities in Frankia, under non-nitrogen fixing conditions, are among the highest reported in prokaryotes. A sixfold increase in SOD activity (2150 units/mg of protein) and a twofold increase in CAT activity (413 units/mg of protein) was seen in cultures derepressed for nitrogen fixation compared with ammonium-grown cultures. Frankia possesses a manganese-containing SOD (MnSOD) when grown with ammonium as the nitrogen source. An additional SOD isozyme containing iron (FeSOD) was detected in cell extracts when grown with dinitrogen. Methyl viologen induced higher activity of the MnSOD in ammonium-grown cultures but failed to induce the FeSOD. During derepression of nitrogenase activity, the simultaneous induction of SOD and CAT was detected. The presence of a new iron-containing SOD under nitrogen-fixing conditions could provide an additional mechanism for protection from O 2 toxicity.
Brain Endo‐Oligopeptidase A, a Putative Enkephalin Converting Enzyme (1987)

Endo-oligopeptidase A, highly purified from the cytosol fraction of bovine brain by immunoaffinity chromatography, has been characterized as a thiol endopeptidase. This enzyme, known to hydrolyze the Phe5-Ser6 bond of bradykinin and the Arg8-Arg9 bond of neurotensin, has been shown to produce, by a single cleavage, Leu5-enkephalin or Met5-enkephalin from small enkephalin-containing peptides. Enkephalin formation could be inhibited in a concentration-dependent manner by the alternative substrate bradykinin. The optimal substrate size was found to be eight to 13 amino acids, with enkephalin the only product released from precursors in which this sequence is immediately followed by a pair of basic residues. However, the specificity constants (kcat/Km) obtained for endo-oligopeptidase A hydrolysis of bradykinin, neurotensin, and dynorphin B are of the same order, a result indicating that the substrate amino acid sequence is not the only factor determining the cleavage site of this enzyme.
Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use (2003)

This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human progenitor cells requires specific combinations of cytokines to promote their proliferation and maturation in vitro. Thrombopoietin (TPO) is the primary regulator of megakaryocytopoiesis (-). Other cytokines, including interleukin-3 (IL-3), IL-6, IL-11, stem cell factor (SCF; also known as kit- ligand), and granulocyte-macrophage colony stimulating factor (GM-CSF) are also capable of promoting megakaryocytopoiesis and will synergize with TPO to increase megakaryocyte proliferation (reviewed in ref. ).
Theoretical study on SAW characteristics of layered structures including a diamond layer (1995)

Diamond has the highest surface acoustic wave (SAW) velocity among all materials and thus can provide much advantage for fabrication of high frequency SAW devices when it is combined with a piezoelectric thin film. Basic SAW properties of layered structures consisting of a piezoelectric material layer, a diamond layer and a substrate were examined by theoretical calculation. Rayleigh mode SAW's with large SAW velocities up to 12,000 m/s and large electro-mechanical coupling coefficients from 1 to 11% were found to propagate in ZnO/diamond/Si, LiNbO/sub 3//diamond/Si and LiTaO/sub 3//diamond/Si structures. It was also found that a SiO/sub 2//ZnO/diamond/Si structure can realize a zero temperature coefficient of frequency with a high phase velocity of 8,000-9,000 m/s and a large electro-mechanical coupling coefficient of up to 4%.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Arabinogalactan for Hepatic Drug Delivery (1994)

Arabinogalactan, a polysaccharide from the tree Larix occidentalis, has been purified and its biological and physical properties described. Intravenous injection of radiolabeled arabinogalactan (4 mg/kg) in rats resulted in 52.5% of the dose being present in the liver, while prior injection of asialofetuin (100 mg/kg) reduced hepatic radioactivity to 3.54%. Gel chromatography indicates arabinogalactan is a single species of 19 kDa, while light scattering gave a molecular weight of 40 kDa. Glycosyl linkage analysis of arabinogalactan is consistent with a highly branched structure comprising a backbone of 1,3-linked galactopyranose connected by 1,3-glycosidic linkages, comprised of 3,4,6-,3,6-, and 3,4- as well as 3-linked residues. In the carbon-13 NMR spectra, the major resonances of arabinogalactan are assigned to beta-galactopyranose, beta-arabinofuranose, and beta-arabinopyranose. Arabinogalactan produced no adverse reactions in single intravenous dose (mouse, 5000 mg/kg) and repeat dose toxicity studies (rats, 500 mg/kg/day, 90 days). When tritiated arabinogalactan was injected, radioactivity cleared from the liver with a half-life of 3.42 days. Arabinogalactan has properties that make it suitable as a carrier for delivering diagnostic or therapeutic agents to hepatocytes via the asialoglycoprotein receptor.
Effect of TiO 2 Nanotubes on Polymer-Fullerene Bulk Heterojunction Solar Cells (2007)

We investigate the photovoltaic properties of hybrid organic solar cell based on the blend of poly[2-methoxy-5-(2_-ethylhexoxy-1,4-phenylenevinylene) (MEH-PPV), C60 and titanium dioxide (TiO2) nanotubes. In comparison of the composite devices with different TiO2:[MEH-PPV +C60] weight ratios of 1wt.% (D1−1), 2wt.% (D1−2), 3wt.% (D1−3), 5wt.% (D1−4), 10wt.% (D1−5) and 20wt.% (D1−6), it is found that the device D1−3 exhibits the best performance. The conversion efficiency is improved by a factor of 3 compared with the MEH-PPV:C60 device.
Fisetin is a senotherapeutic that extends health and lifespan (2018)

The natural product fisetin has senotherapeutic activity in mice and in human tissues. Late life intervention was sufficient to yield a potent health benefit. These characteristics suggest the feasibility to translation to human clinical studies. FUND: NIH grants P01 AG043376 (PDR, LJN), U19 AG056278 (PDR, LJN, WLL), R24 AG047115 (WLL), R37 AG013925 (JLK), R21 AG047984 (JLK), P30 DK050456 (Adipocyte Subcore, JLK), a Glenn Foundation/American Federation for Aging Research (AFAR) BIG Award (JLK), Glenn/AFAR (LJN, CEB), the Ted Nash Long Life and Noaber Foundations (JLK), the Connor Group (JLK), Robert J. and Theresa W. Ryan (JLK), and a Minnesota Partnership Grant (AMAY-UMN#99)-P004610401-1 (JLK, EAA).