gebhart/patrepeval huggingface dataset:
The USTC System for Blizzard Challenge 2008 (2008)

This paper introduces the speech synthesis system developed by USTC for Blizzard Challenge 2008. Two synthetic voices from the released UK English database are built using the HMMbased unit selection synthesis method, which is a hybrid of statistical parametric synthesis and unit-selection techniques. In this method, the optimal sequence of phone-sized candidate units is selected from the database following the statistical criterions derived from a set of trained HMMs for different acoustic features. Then the waveforms of selected units are concatenated to generate the synthesized speech. The evaluation results of Blizzard Challenge 2008 show that our system has good performance on similarity, naturalness and intelligibility for both English voices. Index Terms: speech synthesis, Blizzard Challenge, unit selection, hidden Markov model
The Chd1 chromatin remodeler can sense both entry and exit sides of the nucleosome (2016)

Chromatin remodelers are essential for establishing and maintaining the placement of nucleosomes along genomic DNA. Yet how chromatin remodelers recognize and respond to distinct chromatin environments surrounding nucleosomes is poorly understood. Here, we use Lac repressor as a tool to probe how a DNA-bound factor influences action of the Chd1 remodeler. We show that Chd1 preferentially shifts nucleosomes away from Lac repressor, demonstrating that a DNA-bound factor defines a barrier for nucleosome positioning. Rather than an absolute block in sliding, the barrier effect was achieved by altered rates of nucleosome sliding that biased redistribution of nucleosomes away from the bound Lac repressor site. Remarkably, in addition to slower sliding toward the LacO site, the presence of Lac repressor also stimulated sliding in the opposite direction. These experiments therefore demonstrate that Chd1 responds to the presence of a bound protein on both entry and exit sides of the nucleosome. This sensitivity to both sides of the nucleosome allows for a faster and sharper response than would be possible by responding to only the entry side, and we speculate that dual entry/exit sensitivity is also important for regularly spaced nucleosome arrays generated by Chd1 and the related ISWI remodelers.
Preparation of an Epitaxy-Ready Surface of a ZnO(0001) Substrate (2011)

We show that there can be a contamination of silica particles on the surface of chemo-mechanically polished ZnO substrates. Consideration of the base of electrochemistry led us to a hypothesis that the alkaline polishing solution produces a layer of Zn(OH)2 capturing silica particles. In-situ observation of the etching process in an acidic solution by means of an atomic force microscope as well as zeta potential measurements supports this hypothesis and gives a clear guidance to solve the problem. We find that an epitaxy-ready surface can be reproducibly prepared by dipping ZnO substrates in an HCl solution (HCl:H2O= 7:200) for 30 s.
Fabrication of HTS Josephson junctions on substrates prepared by focused ion beam system (1999)

YBCO Josephson junctions were fabricated by using a Focused Ion Beam (FIB) of 20-nm diameter gallium ion beam. We first made trenches of 100-nm width and 300-nm depth on LaAlO/sub 3/ (LAO) substrates that were covered by 100-nm chromium (Cr) conducting layer. After removing the conducting layer by chemical etching, YBCO followed by a gold layer was pulsed laser deposited in-situ. The deposited YBCO was disconnected over the trenches and the gold layer filled the trenches and made connections between the separated YBCO so that it formed SNS junctions. We observed proximity coupling up to 86 K.
Mechanisms of Dysregulated Antibody Response in Lyme Disease (2020)

OPINION article Front. Cell. Infect. Microbiol., 07 October 2020Sec. Bacteria and Host Volume 10 - 2020 | https://doi.org/10.3389/fcimb.2020.567252
YAP Suppresses Lung Squamous Cell Carcinoma Progression via Deregulation of the DNp63–GPX2 Axis and ROS Accumulation (2017)

Lung squamous cell carcinoma (SCC), accounting for approximately 30% of non-small cell lung cancer, is often refractory to therapy. Screening a small-molecule library, we identified digitoxin as a high potency compound for suppressing human lung SCC growth <i>in vitro</i> and <i>in vivo</i> Mechanistic investigations revealed that digitoxin attenuated YAP phosphorylation and promoted YAP nuclear sequestration. YAP activation led to excessive accumulation of reactive oxygen species (ROS) by downregulating the antioxidant enzyme GPX2 in a manner related to p63 blockade. In patient-derived xenograft models, digitoxin treatment efficiently inhibited lung SCC progression in correlation with reduced expression of YAP. Collectively, our results highlight a novel tumor-suppressor function of YAP via downregulation of GPX2 and ROS accumulation, with potential implications to improve precision medicine of human lung SCC. <i>Cancer Res; 77(21); 5769-81. ©2017 AACR</i>.
Period 2: A Regulator of Multiple Tissue-Specific Circadian Functions (2021)

The zebrafish represents a powerful model for exploring how light regulates the circadian clock due to the direct light sensitivity of its peripheral clocks, a property that is retained even in organ cultures as well as zebrafish-derived cell lines. Light-inducible expression of the <i>per2</i> clock gene has been predicted to play a vital function in relaying light information to the core circadian clock mechanism in many organisms, including zebrafish. To directly test the contribution of <i>per2</i> to circadian clock function in zebrafish, we have generated a loss-of-function <i>per2</i> gene mutation. Our results reveal a tissue-specific role for the <i>per2</i> gene in maintaining rhythmic expression of circadian clock genes, as well as clock-controlled genes, and an impact on the rhythmic behavior of intact zebrafish larvae. Furthermore, we demonstrate that disruption of the <i>per2</i> gene impacts on the circadian regulation of the cell cycle <i>in vivo</i>. Based on these results, we hypothesize that in addition to serving as a central element of the light input pathway to the circadian clock, <i>per2</i> acts as circadian regulator of tissue-specific physiological functions in zebrafish.
Towards the invisible cryogenic system for Magnetic Resonance Imaging (2002)

With about 10,000 Magnetic Resonance Imaging (MRI) systems installed worldwide, helium cooled magnets have become familiar equipment in hospitals and imaging centers. Patients and operators are only aware of the hissing sound of the Gifford-MacMahon refrigerator. Service technicians, however, still work with cryogenic fluids and cold gases, e.g. for replenishing the helium reservoir, inserting retractable current leads for magnet ramps, or replacing burst disks after a magnet quench. We will describe the steps taken at Oxford Magnet Technology towards the ultimate goal of a superconducting magnet being as simple as a household fridge. Early steps included the development of resealing quench valves, as well as permanently installed transfer siphons that only open when fully cooled to 4K. On recently launched 1.5 Tesla solenoid magnets, 500 A current leads are permanently fixed into the service turret, with hardly any boil-off penalty (40–50 cc/hr total). Ramping of the magnet has been fully automated, including electronic supervision of the gas-cooled current leads. One step ahead, the 1 Tesla High Field Open magnet is refrigerated by a single 4K Gifford MacMahon coldhead, relieving the user from the necessity to refill with helium. Our conduction cooled 0.2 Tesla HTS magnet testbed does not require liquid cryogens at any time in its life, including initial cool-down.
Trial of Trazodone for Posttraumatic Stress Disorder Using a Multiple Baseline Group Design (1996)

Six patients with combat-related posttraumatic stress disorder (PTSD) entered a multiple-baseline trial of trazodone, beginning with 50 mg/day and increasing to 400 mg/day until response was maximal. Total Clinician-Administered PTSD Scale scores decreased from a mean of 92 at baseline to 79 at end point, and self-reported PTSD symptoms as measured by the Davidson Trauma Scale paralleled these results (mean of 102 at baseline to 88 at end point). Based on clinician global improvement scores, four patients were rated as much improved and two were rated to be minimally improved. Improvement in social and occupational functioning, and depression was minimal. Available follow-up scores for PTSD symptoms indicated that gains were maintained. Sleep was the first symptom to improve at 2 to 3 months. No dropouts during the treatment period occurred, and reported side effects were quite low. These preliminary data suggest that trazodone may be effective in reducing the three primary clusters of symptoms of PTSD. These findings should be confirmed by using a larger sample in a double-blind, placebo-controlled study.
Electrospun poly-ε-caprolactone scaffold modified with catalytic nitric oxide generation and heparin for small-diameter vascular graft (2017)

Vascular grafts are significantly needed in peripheral vascular surgery; however, small diameter grafts are not always available, and synthetic grafts perform poorly because of acute thrombosis and neointimal proliferation after implantation.
Hemorrhagic Progression of a Contusion after Traumatic Brain Injury: A Review (2011)

The magnitude of damage to cerebral tissues following head trauma is determined by the primary injury, caused by the kinetic energy delivered at the time of impact, plus numerous secondary injury responses that almost inevitably worsen the primary injury. When head trauma results in a cerebral contusion, the hemorrhagic lesion often progresses during the first several hours after impact, either expanding or developing new, non-contiguous hemorrhagic lesions, a phenomenon termed hemorrhagic progression of a contusion (HPC). Because a hemorrhagic contusion marks tissues with essentially total unrecoverable loss of function, and because blood is one of the most toxic substances to which the brain can be exposed, HPC is one of the most severe types of secondary injury encountered following traumatic brain injury (TBI). Historically, HPC has been attributed to continued bleeding of microvessels fractured at the time of primary injury. This concept has given rise to the notion that continued bleeding might be due to overt or latent coagulopathy, prompting attempts to normalize coagulation with agents such as recombinant factor VIIa. Recently, a novel mechanism was postulated to account for HPC that involves delayed, progressive microvascular failure initiated by the impact. Here we review the topic of HPC, we examine data relevant to the concept of a coagulopathy, and we detail emerging data elucidating the mechanism of progressive microvascular failure that predisposes to HPC after head trauma.
Intestinal fatty acid binding protein: folding of fluorescein-modified proteins (1995)

The rat intestinal fatty acid binding protein is an almost all beta-sheet protein that encloses a large interior cavity into which the fatty acid ligand binds. The protein contains neither cysteine nor proline. In a previous report, six site-directed mutants were obtained, each having a single cysteine residue [Jiang, N., & Frieden, C., (1993) Biochemistry 32, 11015-11021] either in a turn or pointed into the cavity. In this report, each mutant has been unfolded in denaturant and modified with 5-iodoacetamido-fluorescein to introduce a large, bulky, and fluorescent group into the protein at a known position. In all cases, fluorescence changes indicated that the modified protein refolded, and circular dichroism measurements suggested that the refolded protein appeared to be mostly beta-sheet. Denaturation curves suggest that for two mutants intermediate structures exist at denaturant concentrations well below the midpoint of the unfolding curve. For each modified, folded protein, one- and two-dimensional 1H NMR spectra were accumulated and compared to the unmodified and wild-type proteins. While the spectra for the modified proteins showed a number of changes in chemical shifts, they were also consistent with folded proteins on the basis of the degree of chemical shift dispersion. Of the six modified mutant proteins, two appear to have the fluorescein group located in the cavity, but only one of these did not bind fatty acid. The remaining modified proteins are capable of ligand binding.(ABSTRACT TRUNCATED AT 250 WORDS)
Abstract IA17: Molecular mechanisms of T-cell exhaustion and implications for immunotherapy (2020)

Abstract T-cell exhaustion is common during chronic infections and cancer and prevents optimal immunity. Exhausted CD8 T cells (TEX) are defined by the loss of ability to perform effector functions efficiently, low proliferative capacity, and poor survival following antigen stimulation. TEX also co-express multiple inhibitory receptors that negatively regulate their function. Indeed, receptors such as PD-1 have become major targets of clinical immunotherapies in cancer and infectious disease aimed at reinvigorating TEX. Despite the clinical success of PD-1-based therapies in human melanoma and other cancers, the majority of patients do not have durable clinical benefit from anti-PD-1 monotherapy. A major challenge remains identifying which patients will respond to anti-PD-1 therapy and defining the underlying mechanisms for successful, durable response versus treatment failure. Detailed immune profiling of peripheral blood from stage IV melanoma patients before and after pembrolizumab (pembro) identified reinvigoration in circulating TEX. These data indicated that nearly 80% of patients experienced a strong, on-target immunologic effect of PD-1 blockade. However, the objective clinical response rate in this cohort was less than 40% and reinvigoration of circulating TEX alone did not predict clinical outcomes. We are currently now using neoadjuvant clinical trial designs and preclinical models to dissect the molecular and mechanistic features of effective and ineffective responses to checkpoint blockade and to understand the underlying molecular program of exhausted CD8 T cells. Our emerging data not only provide a framework for dissecting distinct types of treatment failures in melanoma and have implications for stratifying patients into additional immunotherapeutic treatment approaches, but also point to key underlying molecular pathways involved in programming and controlling the developmental biology of exhausted T cells. The implications of these findings for clinical therapies will be discussed. Citation Format: E. John Wherry. Molecular mechanisms of T-cell exhaustion and implications for immunotherapy [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2018 Nov 27-30; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(4 Suppl):Abstract nr IA17.
Kelompok Industri Kreatif “ Kerupuk Lele Organik ” Di Kecamatan Kademangan, Kanigaran Kota Probolinggo (2019)

Program Kemitraan Masyarakat ini ditujukan untuk masyarakat ekonomi produktif (Usaha Kecil Menengah). Usaha Kecil Menengah yang dimaksud adalah milik Bapak Zainul Abidin (Mitra 1) dan Bapak Hasyim Asyari Fatin (Mitra 2). Kedua Mitra memproduksi Kerupuk Lele Organik. Mitra 1 bertempat di Kecamatan Kademangan Kota Probolinggo. Mitra 2 bertempat di Kecamatan Kanigaran Kota Probolinggo. Kedua mitra memiliki permasalahan yaitu permasalahan produksi dan manajemen keuangan dan organisasi. Permasalahan produksi mitra 1 adalah lamanya proses pemotongan Kerupuk Lele Organik yakni berbentuk gelondong. Permasalahan produksi mitra 2 adalah lamanya mengeringkan Kerupuk Lele Organik dimana tergantung panas matahari. Permasalahan manajemen kedua mitra adalah masih belum mencapai optimal pada sistem manajemen keuangan serta organisasi. Luaran dari program ini berupa mesin untuk pemotong Kerupuk Lele Organik, mesin untuk oven Kerupuk Lele Organik, pelatihan pelatihan manajemen keuangan dan organisasi, peningkatan dalam hal kuantitas dan kualitas produk, dan draf jurnal ilmiah dari hasil progam ini. Hasil dan luaran yang dicapai dalam laporan kemajuan ini adalah persiapan, diskusi, dan proses pengumpulan referensi, mendesain dan membuat mesin pemotongan Kerupuk Lele Organik gelondongan dan mesin untuk oven Kerupuk Lele Organik. Rencana tahapan berikutnya adalah menguji coba dan ditambah mengevaluasi mesin, dan pelatihan manajemen.
Utility of sperm DNA fragmentation testing in different clinical scenarios of male reproductive abnormalities and its influence in natural and assisted reproduction (2017)

One of the measurements of sperm quality that can be assessed with validated protocols is DNA fragmentation, the subject was profoundly dissected in the article by Agarwal et al. and that constitutes the backbone of this commentary (1).
TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins (2016)

TDP-43 protein plays an important role in regulating transcriptional repression, RNA metabolism, and splicing. Typically it shuttles between the nucleus and the cytoplasm to perform its functions, while abnormal cytoplasmic aggregation of TDP-43 has been associated with neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). For the purpose of this study we selected a set of proteins that were misregulated following silencing of TDP-43 and analysed their expression in a TDP-43-aggregation model cell line HEK293 Flp-in Flag-TDP-43-12x-Q/N F4L. Following TDP-43 sequestration in insoluble aggregates, we observed higher nuclear levels of EIF4A3, and POLDIP3β, whereas nuclear levels of DNMT3A, HNRNPA3, PABPC1 and POLDIP3α dropped, and cytoplasmic levels of RANBP1 dropped. In addition, immunofluorescence signal intensity quantifications showed increased nuclear expression of HNRNPL and YARS, and downregulation of cytoplasmic DPCD. Furthermore, cytoplasmic levels of predominantly nuclear protein ALYREF increased. In conclusion, by identifying a common set of proteins that are differentially expressed in a similar manner in these two different conditions, we show that TDP-43 aggregation has a comparable effect to TDP-43 knockdown.
NF-κB DNA binding activity of sesquiterpene lactones fromAnthemis arvensisandAnthemis cotula (2010)

Previous investigations of Anthemis arvensis L. and Anthemis cotula L., species growing wild in Serbia, revealed linear sesquiterpene lactones (SLs) with unusual types of skeleton, named antheindurolides and anthecotuloides. The NF-κB DNA binding activity of four of those SLs as well as from compounds representing structural parts of the SLs is reported in this article. The relationship between their structure and NF-κB inhibition potency is briefly discussed.
Enantioselective Radical Addition/Trapping Reactions with α,β-Disubstituted Unsaturated Imides. Synthesis ofanti-Propionate Aldols (2005)

This manuscript describes a highly diastereo- and enantioselective intermolecular radical addition/hydrogen atom transfer to alpha,beta-disubstituted enoates. Additionally, we show that anti-propionate aldol-like products can be easily prepared from alpha-methyl-beta-acyloxyenoates in good yields and high diastereo- and enantioselectivities.
Method for Assessing Teat and Udder Hygiene (2008)

A new method was developed to quantify bacteria on bovine teats prior to milking. Previous methods using swabs to recover bacteria from teat skin have shown a high degree of variability in the amount of bacteria recovered depending on the amount of pressure applied to the teat/swab interface, the variability in the surface area of the teat swabbed, and the choice of the area to swab as the entire teat surface cannot be practically swabbed. This new method uses a single towel moistened with water to recover soil and bacteria from all four teats of each individual cow. Bacteria are then recovered from the towel and suspended in a sterile water solution. This solution is then cultured and tested using direct microscopic methods. Data are presented from several case studies which were designed to detect differences between different bedding management strategies on the bacteria population on the teats of cows as they entered the milking parlor as well as to detect the efficacy of pre milking teat sanitation in both conventional and automatic (robotic) milking facilities.
Wave form fidelity in pulsed-field capillary electrophoresis (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTWave form fidelity in pulsed-field capillary electrophoresisD. N. Heiger, S. M. Carson, A. S. Cohen, and B. L. KargerCite this: Anal. Chem. 1992, 64, 2, 192–199Publication Date (Print):January 15, 1992Publication History Published online1 May 2002Published inissue 15 January 1992https://pubs.acs.org/doi/10.1021/ac00026a019https://doi.org/10.1021/ac00026a019research-articleACS PublicationsRequest reuse permissionsArticle Views72Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Calcium regulation and bone metabolism : basic and clinical aspects : proceedings of the 10th International Conference on Calcium Regulating Hormones and Bone Metabolism, Montreal, September 9-14, 1989 (1990)

The Sandoz Lecture . Forty six years of involvement with bone and the calcium regulating hormones - a personal odyssey (D.H. Copp). Calciotropic hormones and related peptides and proteins (17 papers). Regulatory actions of vitamin D (9 papers). Bone composition and structure (12 papers). Bone differentation and growth: Factors and cytokines (13 papers). Osteoblast and osteoclast development and metabolism (18 papers). Calcium and bone disorders in man (9 papers). Index of authors. Subject index.
Algal Biomass and Primary Production within a Temperate Zone Sandstone (1987)

The use of dimethyl sulfoxide (DMSO) to extract chlorophyll a and l4C-labelled photosynthate from endolithic algae of sparsely vegetated, cold temperate grasslands on the Colorado Plateau in Arizona has yielded the first estimates of biomass and photosynthesis for this unusual community. These subsurface microorganisms are found widespread in exposed Coconino Sandstone, a predominant formation in this cold temperate region. The endolithic community in Coconino Sandstone, composed primarily of coccoid blue-green and coccoid/sarcinoid green algae, yielded a biomass value (as chlorophyll a content) of 87 mg m-2 rock surface area and a photosynthetic rate of 0.37 mg CO2 dm 2 hr-1 or 0.48 mg CO2 mg-1 chl a hr-1. The endolithic algal community contributes moderate biomass (5–10%) and substantial photosynthesis (20–80%) to the sparse grassland ecosystem.