gebhart/patrepeval huggingface dataset:
Abstract 2191: Imaging the kinetics of targeted nanoparticles to pancreatic cancer (2016)

Abstract Purpose: The purpose of this work is to image the kinetics of targeted lipid nanoparticles to an SCL transporter of pancreatic cancer using a near infrared (NIR) fluorescent dye DyLight® 747 and a mouse orthotopic model of fluorescent pancreatic cancer. Methods: Initially and before the in vivo experiments, the uptake of nanoparticles by human pancreatic cancer cells was evaluated in vitro using fluorescent microscopy and flow cytometry. A quantity of 1.5 x105 PANC-1, MIA PaCa, or BxPC-3 cells/ml was placed in each well of a six-well plate and incubated for 5 hours with different formulations of fluorescent lipid targeted and non-targeted nanoparticles encapsulation daunorubicin which has natural fluorescence. After incubation, the growth media were removed and the cells were washed with PBS. The uptake of the fluorescent lipid nanoparticles by the cancer cells was evaluated an EVOS® microscope. All the in vitro experiments were done in triplicate. For the in vivo targeting studies, nude mice (Balb/c Ola Hsd-Fox1 nu) were implanted orthotopically (in the tail of the pancreas) with 3 x106 PANC-1 cells expressing GFP. The injection volume for the implantation was 50 microliters and contained 3 x106 PANC-1-GFP cells mixed with serum free media and HC Matrigel (1:1). One month after the orthotopic implantation, the implanted pancreatic tumor was visualized in live, anesthetized animals using UVP Scientia (UVP, Upland, CA). The animals were separated into three groups (three animals in each group) and injected via the tail vein as follows: Group A, was injected with only the fluorescent dye, Dylight 747; group B was injected with fluorescent non-targeted nanoparticles; and group C was injected with fluorescent targeted nanoparticles. Results: Both the flow cytometry and fluorescence microscopy data show a significantly higher uptake of the targeted lipid nanoparticles compared to the non-targeted ones. The imaging of the fluorescent lipid nanoparticles in live mice shows that both targeted and nontargeted nanoparticles reach the pancreatic tumor. The nontargeted nanoparticles reside less than 48 hours on the tumor, whereas the targeted nanoparticles can reside for 96 hours on the tumor. The non-encapsulated fluorescent dye did not reach the tumor and was excreted in the urine. Conclusion: The results show that Dylight® 747 can be used to image the kinetics of a drug delivery system in a live animal. Furthermore while both lipid nanoparticles reach the tumor, the targeted lipid nanoparticles reside significantly longer on the tumor and this may translate to a better therapeutic outcome. Citation Format: Jonathan Moreno, Qin qin Fei, Maria P. Lambros. Imaging the kinetics of targeted nanoparticles to pancreatic cancer. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 2191.
INSL3/Leydig Insulin-like Peptide Activates the LGR8 Receptor Important in Testis Descent (2002)

Several orphan G protein-coupled receptors homologous to gonadotropin and thyrotropin receptors have recently been identified and named as LGR4-8. INSL3, also known as Leydig insulin-like peptide or relaxin-like factor, is a relaxin family member expressed in testis Leydig cells and ovarian theca and luteal cells. Male mice mutant for INSL3 exhibit cryptorchidism or defects in testis descent due to abnormal gubernaculum development whereas overexpression of INSL3 induces ovary descent in transgenic females. Because transgenic mice missing the LGR8 gene are also cryptorchid, INSL3 was tested as the ligand for LGR8. Here, we show that treatment with INSL3 stimulated cAMP production in cells expressing recombinant LGR8 but not LGR7. In addition, interactions between INSL3 and LGR8 were demonstrated following ligand receptor cross-linking. Northern blot analysis indicated that the LGR8 transcripts are expressed in gubernaculum whereas treatment of cultured gubernacular cells with INSL3 stimulated cAMP production and thymidine incorporation. The present study identified the ligand for an orphan G protein-coupled receptor based on common phenotypes of ligand and receptor null mice. Demonstration of INSL3 as the ligand for LGR8 facilitates understanding of the mechanism of testis descent and allows studies on the role of INSL3 in gonadal and other physiological processes.
“¿¡Es suficiente una App para encontrarse?!” Investigación exploratoria sobre el uso de las App en la práctica clínica. (2021)

Como terapeutas relacionales sistemicos no podemos ignorar los fenomenos sociales y su evolucion con la innovacion digital. Nuestro trabajo surge de la experiencia como terapeutas que, aficionados a las historias de sus pacientes, se encuentran con historias que implican una nueva forma de relacionarse: la App. Observando como la App puede desempenar un papel importante dentro del contexto terapeutico, a veces llegando a ser un poderoso medio de acceso al mundo del paciente, hemos creado un cuestionario ad hoc, aplicado en linea, para evaluar sus empleos. La nuestra es una investigacion exploratoria que tiene como objetivo observar cuanto espacio toman las App en la practica clinica, proporcionando una lectura cualitativa de esta nueva variable que acaba jugando un papel en la relacion con los pacientes, ajustandose en la danza terapeutica y entrelazandose con la formacion, curiosidad y creatividad del terapeuta.
Molecular recognition directed self‐assembly of supramolecular liquid crystalline polymers from complementary chiral components (1990)

The self‐assembly of intelligent supermolecular materials , for example, liquid crystalline polymers, is the subject of this communication. Employing units based on tartaric acid in the molecular recognition process (see figure) allows the effects of chirality to be investigated as the recognition which occurs at a molecular level is reflected in the properties of the macroscopic mesophases formed. equation image
New directions in FT-Raman spectroscopy (1994)

Although FT-Raman spectroscopy has proven to be applicable to a wide variety of applications, there remains a small number of samples which are not easily analyzed with the technique. Several sampling accessories which have been developed to further expand the applications of FT-Raman spectroscopy are demonstrated.
Niobium-containing catalysts for oxydehydrogenation of hydrocarbons and alcohols (2011)

The first methods are developed for introducing niobium(V) into Mg-Al hydrotalcites used as precursors of oxide catalysts for oxydehydrogenation (OD) of alkanes and alcohols. Samples of niobium(V)-containing oxide catalysts are synthesized. Their catalytic properties are studied in oxydehydrogenation of ethane and ethylbenzene to styrene, oxidation dehydrocyclization of octane into ethylbenzene and styrene, and oxydehydrogenation of sec-butanol to ketone (octane-(2)-one). It is ascertained that ethane transformation into ethylene is highly a selective highly process (92–97%) at low temperatures (450–500°C) in the presence of a niobium-containing catalyst; the catalyst is appreciably efficient in ethylbenzene transformation to styrene and dehydrocyclization of n-octane to ethylbenzene and styrene, and in oxydehydrogenation of secbutanol to octane-(2)-one. All the catalysts studied operate stably in OD reactions; no decrease in their activity or selectivity was detected after 50 h operation.
The Host Protein Aquaporin-9 is Required for Efficient Plasmodium falciparum Sporozoite Entry into Human Hepatocytes (2021)

Hepatocyte invasion by <i>Plasmodium</i> sporozoites represents a promising target for innovative antimalarial therapy, but the molecular events mediating this process are still largely uncharacterized. We previously showed that <i>Plasmodium falciparum</i> sporozoite entry into hepatocytes strictly requires CD81. However, CD81-overexpressing human hepatoma cells remain refractory to <i>P. falciparum</i> infection, suggesting the existence of additional host factors necessary for sporozoite entry. Here, through differential transcriptomic analysis of human hepatocytes and hepatoma HepG2-CD81 cells, the transmembrane protein Aquaporin-9 (<i>AQP9</i>) was found to be among the most downregulated genes in hepatoma cells. RNA silencing showed that sporozoite invasion of hepatocytes requires AQP9 expression. AQP9 overexpression in hepatocytes increased their permissiveness to <i>P. falciparum</i>. Moreover, chemical disruption with the AQP9 inhibitor phloretin markedly inhibited hepatocyte infection. Our findings identify AQP9 as a novel host factor required for <i>P. falciparum</i> sporozoite hepatocyte-entry and indicate that AQP9 could be a potential therapeutic target.
Versatile Markovian models for networks with asymmetric TCP sources (2004)

In this paper we use Stochastic Petri Nets (SPNs) to study the interaction of multiple TCP sources that share one or two buffers, thereby considerably extending earlier work. We first consider two sources sharing a buffer and investigate the consequences of two popular assumptions for the loss process in terms of fairness and link utilization. The results obtained by our model are in agreement with existing analytic models or are closer to results obtained by ns-2 simulations. We then study a network consisting of three sources and two buffers and provide evidence that link sharing is approximately minimum-potential-delay-fair in case of equal round-trip times.
Structure of the O-specific polysaccharide ofProteus vulgarisO37 and close serological relatedness of the lipopolysaccharides ofP. vulgarisO37 andP. vulgarisO46 (2001)

The O-specific polysaccharide (O-antigen) of the lipopolysaccharide (LPS) of Proteus vulgaris O37 was studied by (1)H and (13)C nuclear magnetic resonance spectroscopy before and after O-deacetylation and found to be structurally similar to that of P. vulgaris O46 studied earlier. The two polysaccharides have the same carbohydrate backbone and differ in the position and number of the O-acetyl groups only. Studies with O-antisera against the two strains using passive hemolysis test, enzyme immunosorbent assay, and Western blot revealed close serological relatedness of the LPSs of P. vulgaris O37 and O46. The O-acetyl groups were found to be of little importance for manifesting the O-specificity but to interfere with binding of anti-P. vulgaris O37 serum to P. vulgaris O46 antigen. Based on the data obtained, it was proposed to combine the strains studied in one Proteus serogroup O37 as subgroups O37a,37b and O37a,37c. A cross-reactivity of O-antisera against P. vulgaris O37 and O46 was observed with LPSs of three more Proteus strains, which could be substantiated by the presence of a common disaccharide fragment in the O-antigens.
HydroCrawler – An innovative autonomous surface vehicle for high precision underwater measurements (2019)

Unmanned or autonomous surface vehicles (USV's/ ASV's) are an important class of vehicles for hydrological surveys in shallow waters. In contrast to a diving autonomous underwater vehicle (AUV), the surface vehicle always provides an accurate and absolute position via global navigation satellite system (GNSS). Additionally, the operator has a permanent and broadband radio connection to the surface vehicle and its sensors. These advantages make surface vehicles very popular for hydrological or mapping missions in shallow waters. With this publication, we introduce the HydroCrawler USV - an autonomous measurement platform based on a quadropod-like buoyancy structure, optimized for high floating stability and accuracy but nevertheless flexible positioning. The platform is equipped with a high frequency multibeam echosounder, a video camera, a sediment profiler as well as a multi-parameter hydrological probe. For special solutions, more than 100 kg of additional mission specific payload - like a sampler, winch or manipulator - can be attached to the HydroCrawler. The system was developed for high-resolution bottom mapping of lakes and volumetric reconstructions of underwater archeological sites (e.g. pile dwelling settlements). Its control system allows for repetitive automated grid and track missions and - with the RTK-GNSS system - a reconstruction in absolute coordinates to visualize the sedimentation changes on the seafloor and the degradation of archeological remains over time.
3-Alkyl-3-phenylpiperidine Derivatives as Analgesics. II (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT3-Alkyl-3-phenylpiperidine Derivatives as Analgesics. IIHiroshi Kugita, Toyonari Oine, Hirozumi Inoue, and Goro HayashiCite this: J. Med. Chem. 1965, 8, 3, 313–316Publication Date (Print):May 1, 1965Publication History Published online1 May 2002Published inissue 1 May 1965https://doi.org/10.1021/jm00327a008RIGHTS & PERMISSIONSArticle Views73Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (482 KB) Get e-Alerts Get e-Alerts
The 99m technetium labeling effect on bacterial surface properties. (1987)

The present study was undertaken to determine whether labeling Pseudomonas aeruginosa with 99m Technetium would modify some of the bacterial physicochemical surface properties which play an important role in the interaction between bacteria and eukaryotic cells. No significant difference in electrophoretic mobility or distribution of cationized ferritin on the cell surface was observed between labeled and unlabeled bacteria. Also, the 99m Tc labeling process did not modify bacterial hydrophobicity or adhesiveness to human buccal epithelial cells. It is concluded that bacterial labeling with 99m Tc can be accepted as a useful method for biological research.
5α-Reductase activity in stroma and epithelium of rat prostate and epididymis. A contribution to elucidation of the mechanism for development of hyperplastic growth of prostatic tissue (1983)

The 5 alpha-reductase activity was assayed in homogenates of stroma and epithelium in the rat ventral prostate and epididymis. Samples consisting of a 0.3 mg/ml tissue protein in TES buffer, pH 7.0 were incubated at 37 degrees C for 30 min in the presence of 50 nM [1,2-3H]testosterone and a NADPH-generating system started with 5 x 10(-4) M NADP. The yield of 5 alpha-reduced metabolites, as established by using thin-layer chromatography, gave an estimate of enzyme activity. Whereas the specific activity of 5 alpha-reductase was highest in prostatic stroma and epididymal epithelium, most of the total enzyme activity was associated with the epithelium in both the prostate and epididymis. The effect of dihydrotestosterone on specific activity of 5 alpha-reductase was studied by administering the hormone to 7-day castrated rats. In prostate, the specific activity of both stromal and epithelium forms of the enzyme reached a maximum after 4 days of treatment. In epididymis only the epithelial form of 5 alpha-reductase underwent a major change in specific activity, the latter peaking after 8-12 days of treatment. Furthermore, while the total activity of 5 alpha-reductase in the prostatic tissue fractions could be induced by as much as 4-fold the normal control values, the epididymal enzyme could not be induced above the normal level either in the stroma or the epithelium. This may explain the relative resistance of epididymis to abnormal growth stimulation under the influence of hormones.
Performance comparison of singly-polarised and polarisation-multiplexed coherent transmission at 10Gbauds under linear impairments (2007)

The tolerance to narrow filtering and polarisation mode dispersion is measured with and without polarisation multiplexing at 10Gbauds based on QPSK format. Only a limited penalty is induced by polarisation multiplexing, despite doubling of the channel rate
Integration challenges of new materials and device architectures for IC applications (2004)

In this paper, we will detail the issues with new materials being introduced into CMOS devices and present some potential solutions to enable high performance and low power CMOS for the 65nm node and beyond.
A common multi-agent testbed for diverse seamless personal information networking applications (1998)

This article describes the design and implementation of a unique cooperative agents testbed aimed at addressing diverse applications for the difficult problem of seamless personal information networking (SPIN). The real-world SPIN testbed is aimed at two difficult applications. Namely seamless messaging and intelligent network management. Both applications are agent-driven and share agent behavior, and the messaging agents rely on the network management device diagnostic agents for input. The article introduces both problem areas in a common testbed. The first-generation seamless messaging application is described in detail. User-centric seamless messaging assumes heterogeneous communication environments intended to support today's nomadic users. The prototype is introduced for the management of messages across distributed information networks. Its aim is to intercept, filter, interpret, and deliver multimodal messages, be they voice, fax, video, and/or e-mail messages. A user's personal communication agent is charged with delivering messages to the recipient regardless of their target messaging device-a telephone, pager, desktop, wireless laptop, or wireless phone. Personal communication agents classify and act on incoming messages based on their content. A secretary agent routes and tailors urgent messages appropriately to the device manager agent, which delivers the message to a device on which the user may be roaming or active. What makes the seamless messaging application unique is its approach to treating a message in a universal manner, and its ability to mediate between different messaging environments and devices, and to try to track and find the user.
Sistema fiscal em Portugal: importância do subsetor da contabilidade (2015)

A investigação tem como objetivo conhecer a importância que o subsetor económico da «Contabilidade, auditoria e consultoria» tem assumido recentemente em Portugal, face ao elevado dinamismo da área da fiscalidade, tendo por base o conhecimento do sistema fiscal vigente. Como metodologia de investigação propõe-se, para a análise teórica, uma revisão de literatura, através de pesquisa bibliográfica, para contextualizar o sistema fiscal nacional, dando ênfase aos impostos que mais contribuem para as receitas fiscais. Para a análise empírica, propõe-se uma pesquisa documental apoiada em dados estatísticos publicados pelo Instituto Nacional de Estatística (INE) no período 2008-2012. Os resultados evidenciam que a complexidade do sistema fiscal em Portugal justifica o peso significativo que a área da «Contabilidade, auditoria e consultoria», apoiada em estratégias que assentam em critérios de eficiência, eficácia e economia, tem nos serviços prestados às empresas.
Receiver Initiated Network Allocation Vector Clearing Method in WLANs (2005)

802.11DCF defines a virtual carrier sense mechanism, via RTS/CTS handshaking, to reserve wireless channel. All the nodes overhearing either RTS or CTS set their network allocation vector (NAV) respectively, and defer the channel access for the expected time to finish the packet sequence transmission. The problems arise when RTS or CTS is not successfully received by the addressed node, which causes the channel waste resulted from unnecessary NAV setting. We specify them as RTS-induced and CTS-induced unnecessary NAV setting problems depending on the type of the packet where NAV is set from. Some literatures resolve the problem by channel sensing, though, they are focusing on RTS-induced problems, and infeasible in CTS-induced case due to the unavailability of transmission status at those nodes surrounding the destination. Besides, taking into consideration the path loss, multipath fading, movement, etc., the packet error rate of CTS becomes severe in wireless propagation environment. Therefore, it is necessary to resolve the CTS-induced problem as well. To this end we propose the Receiver Initiated NAV Clearing (RINC) method to enhance the system performance. The method aims to notify surrounding nodes the transmission status via a Clear packet initiated from the receiver. It eliminates the wasted inhibition period in case of broken sequence without interfering with ongoing transmissions. This paper first describes the unnecessary NAV setting problems and further illustrates the MAC operation of the proposed idea. After that, the performance in terms of throughput, average delay, etc. is evaluated by simulation.
Enantioselective Synthesis of Diverse Sulfinamides and Sulfinylferrocenes from Phenylglycine-Derived Chiral Sulfinyl Transfer Agent (2011)

A new chiral sulfinyl transfer auxiliary derived from readily available phenylglycine was developed. This auxiliary is utilized to synthesize a diverse array of alkyl- and arylsulfinamides and sulfinylferrocenes in high yields and excellent ee's. The desired products are produced in a one-pot sequence from the oxathiazolidine 2-oxide by two sequential nucleophilic additions that proceed in a stereospecific manner.