gebhart/patrepeval huggingface dataset:
Characterization of the Effects of Endotoxin on Macrophage Tumor Cell Killing (1978)

Abstract These studies demonstrate the potent effect of LPS on the induction of macrophage-mediated tumor cell killing and show, by using pure populations of cloned macrophages as effector cells, that LPS can act in the complete absence of contaminating lymphocytes. Macrophage differentiation toward the tumoricidal state parallels the responsiveness of macrophages to LPS. Normal macrophages will not kill even in the presence of 10 µg/ml LPS; peptone-induced normal macrophages (stimulated macrophages) are made tumoricidal by ≥500 mg/ml LPS; and nontumoricidal BCG-activated macrophages are made to kill tumor cells by 0.5 to 1.0 ng/ml LPS. The LPS effect is reproduced by lipid A. The effect is abolished by alkaline treatment of LPS or by the presence of polymyxin B. Nontumoricidal BCG-activated macrophages from the lipid A-nonresponder mouse strain C3H/HeJ require 250 times more LPS to become tumoricidal than do nontumoricidal BCG-activated macrophages from the C3H/HeN strain. LPS acts synergistically with MAF-rich peritoneal cell supernatants in causing macrophages to kill tumor cells. Although LPS can elicit MAF production by lymphocytes, it can also act directly on cells of lymphocyte-free macrophage colonies to render them tumoricidal. Reagents are frequently contaminated with LPS, and unless this is noted, valid interpretation of experiments investigating macrophage tumor cell killing is difficult. Means of detecting, detoxifying, and eliminating the LPS of experimental agents are discussed.
Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility (2023)

Formation of the acrosome during spermiogenesis is an essential process for creating fertilization-competent sperm. Of the numerous aspects required for acrosome biogenesis, adherence of the acrosomal outer membrane to the nuclear surface is mediated by the subacrosomal perinuclear theca. However, the cellular dynamics and congruent functions pertaining to these acrosomal anchoring factors are not well understood despite many of them being implicated as potential causes for human male infertility. Actin-like 7A (ACTL7A) is one such factor for which deleterious polymorphisms have recently been shown to cause human male infertility. It is thought that acrosomal attachment is coordinated by cytoskeletal associations between the acrosome and nucleus via the acroplaxome. To further illuminate the mechanistic underpinnings of ACTL7A for essential acrosome associations, in this study, we investigated its dynamic localization in the developing germline, molecular associations with other cytoskeletal components, and the cellular consequences of ablation. Our intracellular localization data show ACTL7A to be dynamically present within the nucleus and subacrosomal space and later associated with postacrosomal regions of developing spermatids. Through the generation of an Actl7a knock-out mouse model, we consistently observed disruption of acrosomal biogenesis with abnormal migration of the acrosomal granule and peeling acrosomes during spermatid elongation. Significantly, we found a complete loss of subacrosomal filamentous actin (F-actin) structures in knock-out spermatids suggesting a regulatory role for subacrosomal F-actin. Considering our reported data together with existing literature, we propose a mechanistic model explaining the essential role of ACTL7A for acroplaxome-associated F-actin, acrosomal attachment integrity, and male fertility.
Microwave Imaging Via Adaptive Beamforming Methods for Breast Cancer Detection (2006)

Abstract Ultra-wideband (UWB) Microwave imaging (MWI) is a promising breast cancer detection technology which exploits the significant contrast in dielectric properties between normal breast tissue and tumor. Previously, data-independent methods, such as delay-and-sum (DAS) and space-time (ST) beamforming, have been used for microwave imaging. However, the low resolution and the poor interference suppression capability associated with the data-independent methods restrict their use in practice, especially when the noise is high and the backscattered signals are weak. In this paper, we develop two data-adaptive methods for microwave imaging, which are referred to as the robust weighted Capon beamforming (RWCB) method and the amplitude and phase estimation (APES) method. Due to their data-adaptive nature, these methods outperform their data-independent counterparts in terms of improved resolution and reduced sidelobe levels.
Patterning and folding of intestinal villi by active mesenchymal dewetting (2023)

Tissue folding generates structural motifs critical to organ function. In the intestine, bending of a flat epithelium into a periodic pattern of folds gives rise to villi, the numerous finger-like protrusions that are essential for nutrient absorption. However, the molecular and mechanical mechanisms driving the initiation and morphogenesis of villi remain a matter of debate. Here, we identify an active mechanical mechanism that simultaneously patterns and folds intestinal villi. We find that PDGFRA+ subepithelial mesenchymal cells generate myosin II-dependent forces sufficient to produce patterned curvature in neighboring tissue interfaces. At the cell-level, this occurs through a process dependent upon matrix metalloproteinase-mediated tissue fluidization and altered cell-ECM adhesion. By combining computational models with <i>in vivo</i> experiments, we reveal these cellular features manifest at the tissue-level as differences in interfacial tensions that promote mesenchymal aggregation and interface bending through a process analogous to the active de-wetting of a thin liquid film.
The first fifty years of ACM SIGCOMM (2019)

Network researchers know that packets are not always evenly spaced, they sometimes arrive in bursts. This burstiness is also present in history. Important events sometimes occur almost simultaneously even if there is no direct relationship between them. Fifty years ago, several historical events took place within a period of a few months. In July, after years of efforts, NASA engineers successfully launched Apollo 13 and the Eagle landed on the Moon. The first footsteps of Neil Armstrong and Buzz Aldrin have opened the era of space exploration and maybe another human will land on another planet during this century. One month later, hundreds of thousands of music fans gathered at the Woodstock festival. In parallel, a few computer scientists and engineers started to deploy prototype nodes of the ARPANET network. The first packets that they exchanged have initiated the revolution that brought the Internet and today's connected society. At that time, research in computer networks had already started and several members of this emerging community gathered in an interest group that later became ACM SIGCOMM.
Recent research progress on cavity-resonator-integrated guided-mode resonance devices (2014)

A cavity-resonator-integrated guided-mode resonance filter (CRIGF) consists of a grating coupler (GC) integrated in a pair of distributed Bragg reflectors (DBRs) on a waveguide and can provide a narrow-band reflection spectrum for an incident wave of a small beam diameter from the free space. The DBRs form a cavity resonator. The GC is the reflection aperture for the incident beam. Effects of grating patterns on the reflection efficiency are discussed. One topic is an improvement of profile matching between the field of an incident beam and the coupling coefficient of GC. A GC with nonuniform fill factors was designed and integrated in order to obtain the profile matching with an incident Gaussian beam of about 10-μm diameter. An efficiency improvement was successfully demonstrated. Another is an investigation of a dependence of the reflection efficiency on a phase-adjusting gap between GC and DBR. The gap is determined to give the maximum reflection efficiency. CRIGFs with different gaps were fabricated and the dependence was experimentally measured to show no serious dependence.
Single cell transcriptional characterization of human megakaryocyte lineage commitment and maturation (2020)

Abstract In the current understanding of adult bone marrow hematopoiesis, megakaryocytes (MKs) originate from cells immuno-phenotypically indistinguishable from hematopoietic stem cells (HSCs), bypassing intermediate progenitors. Here, we use single cell RNA sequencing to characterize HSCs and MKs from human bone marrow, to investigate MK lineage commitment and maturation. We identify two MK primed HSC clusters exhibiting unique differentiation kinetics, at least one of which is used in steady state and stress thrombopoiesis. By analyzing transcriptional signatures we show that human bone marrow MKs originate from MK primed HSC subpopulations, supporting the notion that these display exclusive priming for MK differentiation. We show that transcriptional programs change with increasing MK ploidy, where genes upregulated in high ploidy states may have functional relevance in platelet production. Finally, we highlight the presence of a specific transcriptional signature in MKs from individuals with myocardial infarction, supporting the aberration of MK differentiation in this thrombotic state.
MACOP-B ± radiation therapy for diffuse large cell lymphoma. Analysis of the Stanford Results According to Prognostic Indices (1993)

Patients with limited or low-risk DLCL have an excellent prognosis with MACOP-B +/- RT. These results do not support the use of consolidative high-dose therapy and bone marrow transplantation in patients with limited disease, even if bulky or accompanied by an elevated lactic dehydrogenase. Compared to historical CHOP data, MACOP-B +/- RT does not appear to improve outcome for those patients with poor prognostic features, most of whom will fail. The IPF index is a simple, accurate method of distinguishing high-risk patients who require new therapeutic initiatives.
Syntheses, structures and luminescence properties of five coordination polymers based on designed 2,7-bis(4-benzoic acid)-N-(4-benzoic acid) carbazole (2017)

Herein we report five porous luminescent coordination polymers (CPs), namely, [Zn3(L27)2(DMA)6(H2O)4]n (TCZ-001), [Zn3(L27)2(DMA)6(CH3CH2OH)2]n (TCZ-002), [Zn3(L27)2(DMA)6(CH3OH)]n (TCZ-003), [Cd3(L27)2(DMA)6(H2O)2]n (TCZ-004), and [Cd9(L27)6DMA13(4,4′-BPY)2(OH)2(H2O)13.5]n (TCZ-005) [H3L27 = 2,7-bis(4-benzoic acid)-N-(4-benzoic acid) carbazole, DMA = N,N-dimethylacetamide, 4,4′-BPY = 4,4′-bipyridine, TCZ = “T”-shape carbazole-based polymers, L27 = fully deprotonated H3L273− ligand]. All of the five CPs were assembled from a novel luminescent carbazole-based ligand. X-ray crystallography showed that TCZ-001 is a 3-connected one-dimensional (1D) chain structure with a {42·6} topology. TCZ-002 possesses a 3,6-connected three-dimensional (3D) framework with a point symbol of {42·6}2{44·62·87·102}. TCZ-003 displays a 3,6-connected two-dimensional 2D network with a Schläfli symbol of {411·64}·{43}2, which is a new topology. TCZ-004 features a 3,6-connected 2D net with a {43}2·{46·66·83} topology. In TCZ-005, the structure can be classified into two groups: one of the two groups is a 3-connected 1D chain structure with a {42·6} topology, similar to TCZ-001; the other one possesses a 3,3,3,4-connected network with a Schläfli symbol of {42·63·8}{42·6}3. The structure of TCZ-005 could be described as an SP 1-periodic net (4,4)(0,2) in the 1D_2D.ttd database. TCZ-001, TCZ-003 and TCZ-004 show a remarkable response to the Ni2+ concentration in DMA, H2O or EtOH. Some of Zn2+ ions were replaced by Ni2+ ions and the rate of the transmetalation depended on the concentration of Ni2+ ions. Also, changing part of the metal node would transform the colour of emitted light. Additionally, the colour of the luminescence displays a linear correlation with the Ni2+ concentration range from 0.005 to 0.35 M (mol L−1). Besides selective sensing of Ni2+, TCZ-004 can also be utilized to detect Eu3+ in DMA solution. Thus, several potential sensory probe materials for Ni2+ and Eu3+ detection in DMA solution have been obtained.
Suspended Graphene Membranes with Attached Silicon Proof Masses as Piezoresistive Nanoelectromechanical Systems Accelerometers (2019)

Graphene is an atomically thin material that features unique electrical and mechanical properties, which makes it an extremely promising material for future nanoelectromechanical systems (NEMS). Recently, basic NEMS accelerometer functionality has been demonstrated by utilizing piezoresistive graphene ribbons with suspended silicon proof masses. However, the proposed graphene ribbons have limitations regarding mechanical robustness, manufacturing yield, and the maximum measurement current that can be applied across the ribbons. Here, we report on suspended graphene membranes that are fully clamped at their circumference and have attached silicon proof masses. We demonstrate their utility as piezoresistive NEMS accelerometers, and they are found to be more robust, have longer life span and higher manufacturing yield, can withstand higher measurement currents, and are able to suspend larger silicon proof masses, as compared to the previous graphene ribbon devices. These findings are an important step toward bringing ultraminiaturized piezoresistive graphene NEMS closer toward deployment in emerging applications such as in wearable electronics, biomedical implants, and internet of things (IoT) devices.
Microarray analysis reveals distinctive signaling between the bed nucleus of the stria terminalis, nucleus accumbens, and dorsal striatum (2007)

To identify distinct transcriptional patterns between the major subcortical dopamine targets commonly studied in addiction we studied differences in gene expression between the bed nucleus of the stria terminalis (BNST), nucleus accumbens (NAc), and dorsal striatum (dStr) using microarray analysis. We first tested for differences in expression of genes encoding transcripts for common neurotransmitter systems as well as calcium binding proteins routinely used in neuroanatomical delineation of brain regions. This a priori method revealed differential expression of corticotropin releasing hormone (Crh), the GABA transporter (Slc6a1), and prodynorphin (Pdyn) mRNAs as well as several others. Using a gene ontology tool, functional scoring analysis, and Ingenuity Pathway Analysis, we further identified several physiological pathways that were distinct among these brain regions. These two different analyses both identified calcium signaling, G-coupled protein receptor signaling, and adenylate cyclase-related signaling as significantly different among the BNST, NAc, and dStr. These types of signaling pathways play important roles in, amongst other things, synaptic plasticity. Investigation of differential gene expression revealed several instances that may provide insight into reported differences in synaptic plasticity between these brain regions. The results support other studies suggesting that crucial pathways involved in neurotransmission are distinct among the BNST, NAc, and dStr and provide insight into the potential use of pharmacological agents that may target region-specific signaling pathways. Furthermore, these studies provide a framework for future mouse-mouse comparisons of transcriptional profiles after behavioral/pharmacological manipulation.
Two-Phase Cooling of Power Electronics (2013)

Presentation details two-phase cooling of power electronics.
Triple Crossed Flexure Pivot Based on a Zero Parasitic Center Shift Kinematic Design (2022)

Abstract Thanks to their absence of play, absence of contact friction and possible monolithic fabrication, flexure pivots offer advantages over traditional bearings in small-scale, high accuracy applications and environments where lubrication and wear debris are proscribed. However, they typically present a parasitic center shift that deteriorates their rotational guidance accuracy. Existing solutions addressing this issue have the drawbacks of reducing angular stroke, prohibiting planar design, or introducing overconstraints or underconstraints. This article presents a new triple crossed flexure pivot we have named TRIVOT whose kinematics theoretically nullify its parasitic center shift without overconstraints nor internal mobility. In the physical implementation, the center shift is non-zero but we show using the finite element method (FEM) that it is reduced by one order of magnitude in comparison to the widely used crossed flexure pivot (CFP). This allows to choose a crossing ratio of the flexures that either maximizes the angular stroke limit for given flexures or results in a compact planar design with the possibility of a remote center of compliance (RCC). Based on a pseudo-rigid-body model (PRBM), formulas for the rotational stiffness and angular stroke limit of the TRIVOT are derived, which are then validated by FEM. Finally, we show that a high support stiffness can be achieved based on a preliminary study for a mechanical watch time base application. We expect this new pivot to become a competitive alternative to the standard CFP for applications where high accuracy and compactness are required.
Switched current sigma-delta A/D converter for a CMOS subscriber line analog front end (2002)

This paper describes the design and testing of a 1-bit A/D converter based on the sigma-delta modulation principle. Unlike the conventional realisations using the switched capacitor approach, the current switching technique uses the current level as a variable. In order to check the feasibility and advantages of this technique, a first order 1-bit A/D converter and a second order 1-bit A/D converter were designed. In the first order 1-bit A/D converter a time-continuous current-integrator is used as filter. The circuit has been designed realised and tested. In the second order 1-bit A/D converter the filter is based on the analog sampling and processing of a current signal. The second order filter is implemented with class AB switched current memory cells. The design has been simulated and processed in ES2 1.5 /spl mu/m CMOS technology.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
<title>Brief survey of the relationships between finite random sets and morphology</title> (1994)

In order to be able to optimally design morphological shape extraction algorithms operating on binary digital images, there needs to be a probability theory for finite random sets and probability relations that show how the probability changes as a finite random set is propagated through a morphological operation. In this paper, we develop such a theory for finite random sets. We then demonstrate how to apply this theory for calculating the probability that a set S perturbed by min or max noise N and dilated or eroded by a structuring element K is a subset, superset, or hits a given set R. In some cases we obtain exact results and in some cases we obtain bounds for the desired probability.
Label-Free Bioaerosol Sensing Using Mobile Microscopy and Deep Learning (2018)

Conventional bioaerosol sensing requires the sampled aerosols in the field to be transferred to a laboratory for manual inspection, which can be rather costly and slow, also requiring a professional for labeling and microscopic examination of the samples. Here we demonstrate label-free bioaerosol sensing using a field-portable and cost-effective device based on holographic microscopy and deep-learning, which screens bioaerosols at a throughput of 13 L/min. Two different deep neural networks are designed to rapidly reconstruct the amplitude and phase images of the captured bioaerosols, and to classify the type of each bioaerosol that is imaged. As a proof-of-concept, we studied label-free sensing of common bioaerosol types, for example, Bermuda grass pollen, oak tree pollen, ragweed pollen, Aspergillus spore, and Alternaria spore and achieved >94% classification accuracy. The presented label-free bioaerosol measurement device, with its mobility and cost-effectiveness, will find several applications in indoor and outdoor air quality monitoring.
Changing the game of time resolved X-ray diffraction on the mechanochemistry playground by downsizing (2021)

Time resolved in situ (TRIS) monitoring has revolutionised the study of mechanochemical transformations but has been limited by available data quality. Here we report how a combination of miniaturised grinding jars together with innovations in X-ray powder diffraction data collection and state-of-the-art analysis strategies transform the power of TRIS synchrotron mechanochemical experiments. Accurate phase compositions, comparable to those obtained by ex situ measurements, can be obtained with small sample loadings. Moreover, microstructural parameters (crystal size and microstrain) can be also determined with high confidence. This strategy applies to all chemistries, is readily implemented, and yields high-quality diffraction data even using a low energy synchrotron source. This offers a direct avenue towards the mechanochemical investigation of reactions comprising scarce, expensive, or toxic compounds. Our strategy is applied to model systems, including inorganic, metal-organic, and organic mechanosyntheses, resolves previously misinterpreted mechanisms in mechanochemical syntheses, and promises broad, new directions for mechanochemical research.
Geophysical exploration for stratigraphic oil traps (1943)

Abstract Stratigraphic traps are defined and classified and the applicability of various geophysical methods for the discovery of each of the different types of stratigraphic traps is discussed. Some undeveloped techniques are suggested in this connection as subjects for research.
Verapamil in Unstable Angina Pectoris (1988)

Therapeutic Drug Monitoring is a peer-reviewed, multidisciplinary journal directed to an audience of pharmacologists, clinical chemists, laboratorians, pharmacists, drug researchers and toxicologists. It fosters the exchange of knowledge among the various disciplines–clinical pharmacology, pathology, toxicology, analytical chemistry–that share a common interest in Therapeutic Drug Monitoring. The journal presents studies detailing the various factors that affect the rate and extent drugs are absorbed, metabolized, and excreted. Therapeutic Drug Monitoring is interested in clinical studies that assess and establish pharmacokinetics/pharmacodynamics or toxicokinetics/toxicodynamics relationships. Regular features include review articles on specific classes of drugs, original articles, case reports, technical notes, and continuing education articles. Official Journal of the International Association of Therapeutic Drug Monitoring and Clinical Toxicology
Influence of Natural Asymmetric Embryonic Visual Afferentation on the Neuronal Activations in the Caudomedial Mesopallium during the Freezing Response in Altricial Nestlings (2021)

The study was designed to investigate the role of asymmetric prenatal visual stimulation on the activation of caudomedial mesopallium (CMM) neurons in nine-day-old pied flycatcher nestlings during auditory-guided freezing. Four groups of nestlings were studied: groups 1 and 2 included nestlings with normal vision and visually deprived, respectively, that were incubated and hatched in normal light environment; groups 3 and 4, nestlings with normal vision and visually deprived, respectively, that were incubated and hatched in the dark. The eyes of visually deprived nestlings were covered with non-transparent cups 2 h before the experiment. C-Fos expression was studied. It was shown that densities of neurons activated during freezing response differed in right vs. left CMM only in the group of visually deprived nestlings incubated under light. This suggests that the presence or absence of the asymmetric embryonic visual afferentation may result in the development of different strategies of the visual system integration into defense behavior.