gebhart/patrepeval huggingface dataset:
Bacterial Lipopolysaccharide Regulates the Phosphorylation of the 68K Protein Kinase C Substrate in Macrophages (1989)

Bacterial lipopolysaccharide (LPS) potentiates protein kinase C (PKC)-dependent responses such as the activation of arachidonic acid metabolism in macrophages (Aderem, A. A., Cohen, D. S., Wright, S. D., and Cohn, Z. A. (1986) J. Exp. Med. 164, 165-179). Concomitantly, LPS promotes the myristoylation of a 68K PKC substrate, shown to be equivalent to the 80/87K PKC substrate found in brain and fibroblasts (Aderem, A. A., Albert, K. A., Keum, M. M., Wang, J. K., Greengard, P., and Cohn, Z. A. (1988) Nature 332, 362-364). We have now examined the effect of LPS on the phosphorylation of this 68K PKC substrate. We report here that LPS modifies the kinetics and extent of phosphorylation of the 68K protein. While treatment with LPS alone induces low level phosphorylation of the 68K protein, it markedly increases the rate of subsequent phorbol 12-myristate 13-acetate (PMA)-dependent phosphorylation of this protein. Phosphorylation in LPS-treated macrophages was maximal 1-2 min after administration of PMA, while maximal phosphorylation in macrophages not exposed to LPS was only achieved 6 min after addition of PMA. In addition to increasing the rate of PMA-dependent phosphorylation of the 68K protein in macrophages, LPS also promoted the phosphorylation of a novel peptide on the 68K protein. Thus while PMA stimulated the phosphorylation of two thermolytic phosphopeptides (phosphopeptides 1 and 2), the low level of phosphorylation observed with LPS alone was found to occur on phosphopeptides 1 and 2 as well as on a novel phosphopeptide (phosphopeptide 3). Furthermore, LPS treatment of macrophages potentiated phosphorylation of all three phosphopeptides when the cells were subsequently stimulated with PMA. While phosphorylation stimulated by LPS and PMA was slightly more than additive for phosphopeptides 1 and 2, it was markedly synergistic (increased 14.5-fold) for phosphopeptide 3. Phosphorylation of all three phosphopeptides occurred exclusively on serine. It is possible that LPS-induced myristoylation of the 68K protein directs it to the membrane where its phosphorylation is enhanced by its close association with PKC.
Functional polymers containing dimethylene spacers. Characterization by solid-phase carbon-13 NMR (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleFunctional polymers containing dimethylene spacers. Characterization by solid-phase carbon-13 NMRJian Ping Gao, Frederick G. Morin, and Graham D. DarlingCite this: Macromolecules 1993, 26, 5, 1196–1198Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/ma00057a050https://doi.org/10.1021/ma00057a050research-articleACS PublicationsRequest reuse permissionsArticle Views91Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots (2015)

A novel method for covalent conjugation of DNA to polymer coated quantum dots (QDs) is investigated in detail. This method is fast and efficient: up to 12 DNA strands can be covalently conjugated per QD in optimized reaction conditions. The QD-DNA conjugates can be purified using size exclusion chromatography and the QDs retain high quantum yield and excellent stability after DNA coupling. We explored single-stranded and double-stranded DNA coupling, as well as various lengths. We show that the DNA coupling is most efficient for short (15 mer) single-stranded DNA. The DNA coupling has been performed on QDs emitting at four different wavelengths, as well as on gold nanoparticles, suggesting that this technique can be generalized to a wide range of nanoparticles.
Structural and Optical Investigation of ZnO Nanopowders Synthesized from Zinc Chloride and Zinc Nitrate (2015)

ZnO Nanopowders were synthesized from Zinc Chloride and Zinc Nitrate by Co-precipitation Method. The synthesized ZnO nanopowders were further characterized using X Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) Spectroscopy. The Structure, Morphology and Optical properties were investigated for each precursor. XRD patterns indicate hexagonal wurtzite structure with high crystallinity. SEM images revealed the morphology of the ZnO Particles. The optical characterizations of ZnO nanoparticles have been carried out using FTIR Spectroscopy. The spectrum shows the characteristic absorption peaks of Zn-O are nearer to 390 cm-1 and also authenticates presence of ZnO. From the analyses of results, it is very clear that precursors used in the present study have played a vital role in surface morphology, structural and optical properties of ZnO nanoparticles.
Biomass Properties and Gasification Behavior of 7-Year-Old Siberian Elm (1987)

Studies were conducted to establish baseline information for use in characterizing Siberian elm as energy or fiber feedstock.Biomass properties were determined.Calorific value (4,698 cal/g) and specific gravity (0.55) of Siberian elm are similar to those of soft elms, and its fiber length is relatively short (1 .OO mm).Ash content was 1.65O/o.Gasification produces a medium energy gas (3 x lo6 cal/m3) with yield varying from 0.17 to 0.96 m3/kg over a gasification temperature range of 600 to 700 C. Ovendry yields were 9.8 t/ha annually at 700 trees/ha.
Design and synthesis of novel spin-labeled camptothecin derivatives as potent cytotoxic agents (2020)

In our continuing search for natural product-based spin-labeled antitumor drugs, 20 novel spin-labeled camptothecin derivatives were synthesized via a Cu-catalyzed one pot reaction and evaluated for cytotoxicity against four human tumor cell lines (A-549, MDA-MB-231, KB, and KBvin). Eighteen of the target compounds (9a, 9b, 9d–9k, 9m–9t) exhibited significant in vitro antiproliferative activity against these four tested tumor cell lines. Compounds 9e and 9j (IC50 0.057 and 0.072 μM, respectively) displayed the greatest cytotoxicity against the multidrug-resistant (MDR) KBvin cell line and merit further development into preclinical and clinical drug candidates for treating cancer including MDR phenotype.
A novel therapy for an unusual problem: IL‐1 receptor antagonist for recurrent post‐transplant pericarditis (2019)

Heart transplant (HTx) recipients are at increased risk of pericardial disease. Idiopathic recurrent pericarditis has not been previously described following HTx. We describe a 35-year-old male who was admitted with pericarditis and moderate pericardial effusion 10 months after HTx. Two weeks before his admission, his prednisone had been tapered off. A thorough infectious workup and endomyocardial biopsy was unrevealing. He was started on colchicine with the addition of tapering prednisone regimen of 40 mg daily due to unresolved pain. Over the next several years, he had three recurrent episodes of pericarditis requiring re-initiation of prednisone with extensive investigations negative for rejection, autoimmune, and infectious causes. Cardiac MRI confirmed pericardial inflammation. Due to his recurrent course and inability to wean off prednisone, anakinra, an IL-1 receptor antagonist, was started at 100 mg sc daily. This allowed successful discontinuation of prednisone. He is now 34 months post-transplant without recurrence on anakinra and colchicine maintenance. Due to the overlap between idiopathic recurrent pericarditis and auto-inflammatory diseases, there is growing evidence for utilizing IL-1 receptor antagonists in this condition. While pericarditis is common in the HTx population, this is the first report of successful use of an IL-1 receptor blocker for pericarditis in this population.
A physics-inspired deep learning framework for an efficient FPM reconstruction under low overlap conditions (2023)

2D observation of biological samples at hundreds of nanometers resolution or even below is of high-interest for many sensitive medical applications. Recent advances have been obtained with computational imaging and we consider here Fourier Ptychographic Microscopy (FPM). Although this technique has permitted to attain an impressive super-resolution factor up to 6 and to assess at the same time local optical thickness of samples, it suffers from low measurement cadence. This limits its applicability as a routine diagnostic tool. In this paper, we address the specific problem of FPM reconstruction when only few low resolution images are acquired. To this end, we introduce a physics informed optimization Deep Neural Network that is combined to statistical reconstruction learning. More precisely, the forward image microscope formation model is explicitly introduced in the model to optimize its weights starting from an initialization that is based on a statistical learning. This combination brings some robustness to the reconstruction process. The presented simulation results for an FPM configuration with very low overlap factor (Γ ∼ 10%) demonstrate the conceptual benefits of the approach in terms of image quality and resolution. The learning step is also shown to be mandatory.
An Unscented Kalman Filter Approach to the Estimation of Nonlinear Dynamical Systems Models (2007)

In the past several decades, methodologies used to estimate nonlinear relationships among latent variables have been developed almost exclusively to fit cross-sectional models. We present a relatively new estimation approach, the unscented Kalman filter (UKF), and illustrate its potential as a tool for fitting nonlinear dynamic models in two ways: (1) as a building block for approximating the log-likelihood of nonlinear state-space models and (2) to fit time-varying dynamic models wherein parameters are represented and estimated online as other latent variables. Furthermore, the substantive utility of the UKF is demonstrated using simulated examples of (1) the classical predator-prey model with time series and multiple-subject data, (2) the chaotic Lorenz system and (3) an empirical example of dyadic interaction.
RAPIDO: a web server for the alignment of protein structures in the presence of conformational changes (2008)

Rapid alignment of proteins in terms of domains (RAPIDO) is a web server for the 3D alignment of crystal structures of different protein molecules in the presence of conformational change. The structural alignment algorithm identifies groups of equivalent atoms whose interatomic distances are constant (within a defined tolerance) in the two structures being compared and considers these groups of atoms as rigid bodies. In addition to the functionalities provided by existing tools, RAPIDO can identify structurally equivalent regions also when these consist of fragments that are distant in terms of sequence and separated by other movable domains. Furthermore, RAPIDO takes the variation in the reliability of atomic coordinates into account in the comparison of distances between equivalent atoms by employing weighting-functions based on the refined B-values. The regions identified as equivalent by RAPIDO furnish reliable sets of residues for the superposition of the two structures for subsequent detailed analysis. The RAPIDO server, with related documentation, is available at http://webapps.embl-hamburg.de/rapido.
[Effect of betotastine besilate (TAU-284), a novel anti-allergic agent, on experimental allergic rhinitis]. (1997)

We investigated the effect of betotastine besilate (betotastine) on the experimental allergic rhinitis. The oral administration of betotastine (1, 3 and 10 mg/kg) inhibited the increase in dye leakage during and after the nasal perfusion of antigen in actively sensitized rats. It also prevented the increase in intranasal pressure induced by topically applied histamine in non-sensitized guinea pigs. Cetirizine and terfenadine dose-dependently inhibited the increase in a similar manner. Ketotifen (0.01-0.3 mg/kg, p.o.) inhibited the increase more than 50% at 0.01 mg/kg. The ID50s of ketotifen, cetirizine, betotastine and terfenadine for this model were more than 0.01 mg/kg, 0.01 mg/kg, 0.03 mg/kg and 0.5 mg/kg, respectively. Furthermore, in actively sensitized guinea pigs, nasal airway resistance showed a biphasic increase after the topical antigen challenge to the nasal cavity; the first peak at 0.5 hr and a second peak at 4 hr. Both the responses of first and second peaks were significantly inhibited by orally administered betotastine besilate, and its inhibitory effect on the second peak was the strongest among drugs tested. Since betotastine showed significantly inhibitory effects in experimental allergic rhinitis models, it was suggested to show a good efficacy for the treatment of allergic rhinitis clinically.
Histamine Deficiency Decreases Atherosclerosis and Inflammatory Response in Apolipoprotein E Knockout Mice Independently of Serum Cholesterol Level (2011)

These results indicate that histamine is involved in the development of atherosclerosis in apoE-KO mice by regulating gene expression of inflammatory modulators, an action that appears to be independent of serum cholesterol levels. In addition to acute inflammatory response, histamine participates in chronic inflammation, such as hyperlipidemia-induced atherosclerosis, and might be a novel therapeutic target for the treatment of atherosclerosis.
Real-time estimation and tracking of optical flow vectors for obstacle detection (2002)

Optical flow contains information about the motion of a camera relative to its environment and about the three-dimensional structure of the imaged scene. In this contribution we use that information to detect obstacles in front of a moving vehicle. Since the detection is based on motion no a-priori knowledge about obstacle shape is required. Optical flow vectors are estimated from spatio-temporal derivatives of the gray value function which are computed at video frame rate by the custom-designed hardware MiniVISTA. To eliminate outliers and to speed up obstacle detection by data reduction the estimated vectors are clustered before they are passed to the obstacle test. The purpose of the obstacle test is to separate moving objects from the stationary environment and to separate elevated objects from the ground plane. In continuation of our previous work, obstacle detection is regarded as a state estimation problem. This enables us to enlarge the motion stereo basis by applying a Kalman filter to track optical flow vectors over subsequent image frames. Experimental results obtained from image sequences recorded with our experimental vehicle are presented.
Fundamentals of Boundary-Layer Heat Transfer With Streamwise Temperature Variations (1962)

International audience
Epitaxially grown III-arsenide-antimonide nanowires for optoelectronic applications (2019)

Epitaxially grown ternary III-arsenide-antimonide (III-As-Sb) nanowires (NWs) are increasingly attracting attention due to their feasibility as a platform for the integration of largely lattice-mismatched antimonide-based heterostructures while preserving the high crystal quality. This and the inherent bandgap tuning flexibility of III-As-Sb in the near- and mid-infrared wavelength regions are important and auspicious premises for a variety of optoelectronic applications. In this review, we summarize the current understanding of the nucleation, morphology-change and crystal phase evolution of GaAsSb and InAsSb NWs and their characterization, especially in relation to Sb incorporation during growth. By linking these findings to the optical properties in such ternary NWs and their heterostructures, a brief account of the ongoing development of III-As-Sb NW-based photodetectors and light emitters is also given.
Sound propagation in normal and superfluid $sup 3$He (1975)

Liquid /sup 3/He, despite the rather simple nature of its interatomic force, displays striking properties at ultralow temperatures. Below about 2 x 10/sup -3/ K it behaves as a superfluid whose properties are related to, but are much more varied and complex than, those of superconducting electrons in metals. Ultrasonic waves prove to be a powerful probe of this system; here the acoustic properties of both the normal and superfluid phases of this liquid are briefly reviewed.
On the Stability of Liposomes and Catansomes in Aqueous Alcohol Solutions (2008)

In this work, a systematic study of effects of three cosolvents (methanol, ethanol, and 1-propanol) on the stability of liposomes formed from soybean phosphatidylcholine (PL-90) by a semispontaneous process was carried out. The experimental results revealed that significant enhancement of PL-90 liposome stability could be achieved by cosolvent addition with suitable amounts. A similar phenomenon was also observed for catansomes formed from ion-pair amphiphiles (IPAs) as demonstrated by decyltrimethylammonium dodecyl sulfate (DeTMA-DS) with a comparatively high concentration of 5 mM. In general, with continued increase in the cosolvent concentration, the stability of liposomes and catansomes first increased, reached a maximum, and then decreased. Furthermore, it was realized that the cosolvent effects on the liposome stability were very similar to those on the catansome stability, which might be also explained by the mechanism proposed on the basis of the viewpoint of a mixed solvent dielectric constant.
Metabolite proton T2 mapping in the healthy rhesus macaque brain at 3 T (2009)

The structure and metabolism of the rhesus macaque brain, an advanced model for neurologic diseases and their treatment response, is often studied noninvasively with MRI and (1)H-MR spectroscopy. Due to the shorter transverse relaxation time (T(2)) at the higher magnetic fields these studies favor, the echo times used in (1)H-MR spectroscopy subject the metabolites to unknown T(2) weighting, decreasing the accuracy of quantification which is key for inter- and intra-animal comparisons. To establish the "baseline" (healthy animal) T(2) values, we mapped them for the three main metabolites' T(2)s at 3 T in four healthy rhesus macaques and tested the hypotheses that their mean values are similar (i) among animals; and (ii) to analogs regions in the human brain. This was done with three-dimensional multivoxel (1)H-MR spectroscopy at (0.6 x 0.6 x 0.5 cm)(3) = 180 microL spatial resolution over a 4.2 x 3.0 x 2.0 = 25 cm(3) ( approximately 30%) of the macaque brain in a two-point protocol that optimizes T(2) precision per unit time. The estimated T(2)s in several gray and white matter regions are all within 10% of those reported in the human brain (mean +/- standard error of the mean): N-acetylaspartate = 316 +/- 7, creatine = 177 +/- 3, and choline = 264 +/- 9 ms, with no statistically significant gray versus white matter differences.
Spatial encryption using a retrodirective antenna array (2009)

In this paper we show how a 2.4GHz retrodirective array operating in a multipath rich environment can be utilised in order to spatially encrypt digitally encoded data. For the first time we show experimentally that digital data which has no mathematical encryption applied to it can be successfully recovered only when it is detected within a highly constrained spatial region co-located close to the position of the CW reference signal. This means that the signal cannot be intercepted and its modulated data recovered at locations other than the constrained spatial region around the position from which the retrodirective communication was initiated. Thus we say that it is spatially encrypted.
Pelvic Floor Dysfunction in Aging Women (2007)

The occurrence of pelvic floor dysfunction may increase steadily during the aging process in women. Pelvic floor dysfunction may be associated with dysfunctions of micturition, defecation, prolapse, and sex. The natural history and mechanism of pelvic floor dysfunction in aged women are not well understood or explored. In this article, we review the effect of age on the prevalence of pelvic floor dysfunction and on the structural and functional changes of the lower urinary tract, anorectum and pelvic floor. Altogether, the aging process has a negative impact on either the function or structure of the lower urinary tract, anorectum and pelvic floor in women.
Homocystein beim Alkoholismus: Pathobiochemische und pathophysiologische Aspekte (2004)

&lt;B&gt;Hintergrund:&lt;/B&gt; Eine Alkoholismus-&#x0D; assoziierte Hyperhomocysteinämie kommt nicht nur im Alkoholentzug vor, sondern ist mit kontinuierlichem und chronischem Alkoholkonsum verknüpft. Bei einer unter Alkoholentzug verstärkten glutamatergen Exzitotoxizität und Stress-induzierten Permeabilisierung der Blut-Hirn-Schranke erlauben erhöhte Homocystein-Plasmakonzentrationen die Prädiktion von Alkoholentzugsanfällen, was dann die frühzeitige Einleitung einer medikamentösen antiepileptischen Prophylaxe ermöglicht. Die Alkoholismus-induzierte Hyperhomocysteinämie korreliert mit der hippokampalen Volumenreduktion bei Patienten mit Alkoholismus und könnte zur Pathogenese auch der Alkohol-bedingten Hirnatrophie beitragen. Eine Hyperhomocysteinämie-induzierte Dysregulation der epigenetischen Kontrolle wird aktuell untersucht.&#x0D; &lt;/P&gt;&lt;P&gt;&#x0D; &lt;B&gt;Schlussfolgerungen:&lt;/B&gt; Prospektive Interventionsstudien werden klären, ob durch eine frühzeitige Senkung erhöhter Homocysteinspiegel die Inzidenz von Komplikationen des Alkoholentzuges oder Alkoholismus-induzierter chronischer Schäden reduziert werden kann.