gebhart/patrepeval huggingface dataset:
Sensitization to bradykinin B1 and B2 receptor activation in UV-B irradiated human skin (2004)

Bradykinin B1 and B2 receptors contribute to nociceptor sensitization under inflammatory conditions. Here, we examined the vascular inflammatory responses and nociceptive effects resulting from activation of B1 and B2 receptors in healthy and UV-B irradiated skin in human volunteers. The B1 receptor agonist des-Arg(10)-Kallidin (10(-6)-10(-3)M) and the B2 receptor agonist bradykinin (10(-9)-10(-4)M) were administered by dermal microdialysis to the ventral thigh. UV-B irradiation was performed 24 h prior to the experiment with the threefold minimum erythemal dose. Pain sensation perceived during the stimulation with the bradykinin receptor agonists was estimated on a numeric scale. Local and axon reflex-induced vasodilations were recorded by laser Doppler imaging. For protein extravasation, total protein content in the dialysate was assessed as a measure of increased endothelial permeability. In normal skin, both B1 and B2 receptor activation dose-dependently evoked pain, vasodilatation and protein extravasation. In UV-B irradiated skin, pain sensation and axon reflex vasodilatation were enhanced by both B1 and B2 agonists, whereas local vasodilatation was increased only following B1 receptor activation. The UV-B irradiation did not enhance B1 and B2 receptor-induced protein extravasation indicating a differential sensitization of the neuronal, but not the vascular response.
C -Reactive Protein, Leukocyte Count and Neutrophils: A Diagnostic Aid in Acute Appendicitis (2023)

Background: Malnutrition is known to be a poor prognostic factor affecting the outcome of pediatric cancers. The objective of this study was to assess the pre-existing malnutrition in newly diagnosed pediatric cancer patients presenting at the Pediatric Oncology Department, Children Hospital, PIMS and their number of hospital admissions due to causes other than chemotherapy.&#x0D; Methodology: Data of 44 newly diagnosed children with cancer was analyzed to find out the association of nutritional status according to z-score for weight and height for age, body mass index (BMI) and mid-upper arm circumference (MUAC) with their number of hospital admissions for 6 months since their date of diagnosis.&#x0D; Results: The mean age of the study subjects was 4.25 ± 2.85 years, out of which 33(75%) were males and 11(25%) females. Most of the patients were diagnosed with leukemia or lymphoma. Nutritional status evaluation of thirty patients who got admitted was mild to moderate wasting in 24(80%) assessed by weight for age, mild to moderate stunting in 21 (70%) according to height for age and mild to severe malnutrition in 10 (33%) based on body mass index and mid upper arm circumference (MUAC). There was significant association between nutritional status of patients at the time of diagnosis with additional hospitalization with p value less than 0.05&#x0D; Conclusion: Malnutrition at the time of diagnosis is significantly associated with an increase in the number of hospital admissions in pediatric cancer patients.&#x0D; Key words: Hospitalization, Malnutrition, Oncology, Pediatric&#x0D;
Bayesian image reconstruction - The pixon and optimal image modeling (1993)

In this paper we describe the Optimal Image Model, Maximum Residual LIkelihood method (OptMRL) for image reconstruction. OptMRL, like maximum entropy mehtods (ME), is a Bayesian image reconstruction technique for removing point spread function blurring. Like ME, OptMRL uses both a goodness of fit criterion (GOF) and an "image prior," i.e., a function which quantifies the a priori probability of the image. However, unlike standard ME techniques which typically reconstruce the image on the data pixel grid, OptMRL varies the image model in order to find the optimal functional basis with which to represent the iage. In this regard, our method is similar to the multi-channel ME methods proposed by Weir. In this paper, we show how an optimal basis for image representation can be selected and in doing so, develop the concept of the "pixon" which is a generalized image cell from which this basis is constructed. By allowing both the image and the image representation to be variable, the OptMRL method greatly increases the volume of solution space over which the image is optimized. Hence the likelihood of the final reconstructed image is greatly increased. For the goodness of fit criterion, OptMRL uses the Maximum Residual Likelihood probability distribution introduced previously by Pina and Puetter. This GOF probability distribution, which is based on the spatial autocorrelation of the residuals, has the advantage that it ensures spatially uncorrelated image reconstruction residuals.
Effects of Multiple Ligand Binding on Kinetic Isotope Effects in PQQ-Dependent Methanol Dehydrogenase (2003)

The reaction of PQQ-dependent methanol dehydrogenase (MDH) from Methylophilus methylotrophus has been studied by steady-state and stopped-flow kinetic methods, with particular reference to multiple ligand binding and the kinetic isotope effect (KIE) for PQQ reduction. Phenazine ethosulfate (PES; an artificial electron acceptor) and cyanide (a suppressant of endogenous activity), but not ammonium (an activator of MDH), compete for binding at the catalytic methanol-binding site. Cyanide does not activate turnover in M. methylotrophus MDH, as reported previously for the Paracoccus denitrificans enzyme. Activity is dependent on activation by ammonium but is inhibited at high ammonium concentrations. PES and methanol also influence the stimulatory and inhibitory effects of ammonium through competitive binding. Reaction profiles as a function of ammonium and PES concentration differ between methanol and deuterated methanol, owing to force constant effects on the binding of methanol to the stimulatory and inhibitory ammonium binding sites. Differential binding gives rise to unusual KIEs for PQQ reduction as a function of ammonium and PES concentration. The observed KIEs at different ligand concentrations are independent of temperature, consistent with their origin in differential binding affinities of protiated and deuterated substrate at the ammonium binding sites. Stopped-flow studies indicate that enzyme oxidation is not rate-limiting at low ammonium concentrations (<4 mM) during steady-state turnover. At higher ammonium concentrations (>20 mM), the low effective concentration of PES in the active site owing to the competitive binding of ammonium lowers the second-order rate constant for enzyme oxidation, and the oxidative half-reaction becomes more rate limiting. A sequential stopped-flow method is reported that has enabled, for the first time, a detailed study of the reductive half-reaction of MDH and comparison with steady-state data. The limiting rate of PQQ reduction (0.48 s(-1)) is less than the steady-state turnover number, and the observed KIE in stopped-flow studies is unity. Although catalytically active, we propose reduction of the oxidized enzyme generated in stopped-flow analyses is gated by conformational change or ligand exchange. Slow recovery from this trapped state on mixing with methanol accounts for the slow reduction of PQQ and a KIE of 1. This study emphasizes the need for caution in using inflated KIEs, and the temperature dependence of KIEs, as a probe for hydrogen tunneling.
Software Environment for Robust H 2 and H ∞ Controller Tuning and Real Time Control (2006)

This paper presents a novel and flexible software environment for data acquisition and system identification in real time, oriented to process control. Our application implements PID, H2 and H∞ tuning methods for systems whose process reaction curve may be considered that belongs to some of the considered response patrons. A novel procedure for H2 and H∞ controller tuning is employed, valid for SISO and MIMO systems, which is easy to carry out for a plant operator. Evaluation tests are applied, and numerical indicators are used in order to validate the identified mathematical model and the designed controller. A friendly user interface facilities monitoring, analysis and validation tasks.
Scan statistic tail probability assessment based on process covariance and window size (2013)

A scan statistic is examined for the purpose of testing the existence of a global peak in a random process with dependent variables of any distribution. The scan statistic tail probability is obtained based on the covariance of the moving sums process, thereby accounting for the spatial nature of the data as well as the size of the searching window. Exact formulas linking this covariance to the window size and the correlation coefficient are developed under general, common and auto covariance structures of the variables in the original process. The implementation and applicability of the formulas are demonstrated on families of multiple processes of t-statistics. A sensitivity analysis provides further insight into the variant interaction of the tail probability with the influence parameters. An R code for the tail probability computation is offered within the supplementary material.
Dielectric spectroscopy of polymeric materials : fundamentals and applications (1997)

FUNDAMENTALS OF DIELECTRIC MATERIALS 1. Theory of Dielectric Properties 2. Broadband Dielectric Measurement Techniques 3. Dielectric Properties of Amorphous Polymers 4. Dielectric Spectroscopy of Semicrystalline Polymers 5. Calculation of Dipole Moments and Correlation Parameters 6. Unbiased Modeling of Dielectric Dispersions 7. Application High-Frequency Dielectric Measurements of Polymers 8. Thermally Stimulated Currents of Polymers APPLICATION OF DIELECTRIC SPECTROSCOPY TO POLYMER SYSTEMS 9. Dielectric Relaxation in Polymer Solutions 10. Dielectric Studies of Polymer Blends 11. Dielectric Monitoring of Polymerization and Cure 12. Dielectric Properties of Polymeric Liquid Crystals 13. Dielectric Properties of Fluoropolymers 14. Dielectric Studies of Polymeric Nonlinear Optical Materials 15. Broadband Dielectric Spectroscopy on Collective and Molecular Dynamics in Ferroelectric Liquid Crystals
Myosin 1e promotes breast cancer malignancy by enhancing tumor cell proliferation and stimulating tumor cell de-differentiation (2016)

Despite advancing therapies, thousands of women die every year of breast cancer. Myosins, actin-dependent molecular motors, are likely to contribute to tumor formation and metastasis via their effects on cell adhesion and migration and may provide promising new targets for cancer therapies. Using the MMTV-PyMT murine model of breast cancer, we identified Myosin 1e (MYO1E) as a novel tumor promoter. Tumor latency in mice lacking MYO1E was significantly increased, and tumors formed in the absence of MYO1E displayed unusual papillary morphology, with well-differentiated layers of epithelial cells covering fibrovascular cores, rather than solid sheets of tumor cells typically observed in this cancer model. These tumors were reminiscent of papillary breast cancer in humans that is typically non-invasive and often cured by tumor excision. MYO1E-null tumors exhibited decreased expression of the markers of cell proliferation, which was recapitulated in primary tumor cells derived from MYO1E-null mice. In agreement with our findings, meta-analysis of patient survival data indicated that MYO1E expression level was associated with reduced recurrence-free survival in basal-like breast cancer. Overall, our data suggests that MYO1E contributes to breast tumor malignancy and regulates the differentiation and proliferation state of breast tumor cells.
Synthesis of Novel N1 and N2 Indazole Derivatives (2011)

The alkylation of 5‐cyano indazoles 2 in the presence of readily available imidizolium salts (ionic liquids) (Bmim)BF 4 as phase transfer catalyst were performed to afford the regio isomers N 1 ‐and N 2 ‐(substituted alkyl)‐5‐cyano indazoles. The N 1 ‐alkylated cyano indazoles were converted to the corresponding N 1 ‐(substituted alkyl)‐5‐aminomethyl and 5‐carbaldehydes 5(a-f) and 6(a-f) .
A tunable holistic resiliency approach for high-performance computing systems (2009)

In order to address anticipated high failure rates, resiliency characteristics have become an urgent priority for next-generation extreme-scale high-performance computing (HPC) systems. This poster describes our past and ongoing efforts in novel fault resilience technologies for HPC. Presented work includes proactive fault resilience techniques, system and application reliability models and analyses, failure prediction, transparent process- and virtual-machine-level migration, and trade-off models for combining preemptive migration with checkpoint/restart. This poster summarizes our work and puts all individual technologies into context with a proposed holistic fault resilience framework.
Repair of Single-Stranded DNA Nicks, Gaps, and Loops in Mammalian Cells (1987)

We studied the ability of mammalian cells to repair single-stranded nicks, gaps, and loops in DNA duplexes. Heteroduplexes prepared from derivatives of the shuttle vector pSV2neo were introduced into monkey COS cells. After replication, the plasmids were recovered and used to transform Escherichia coli. Plasmid DNA from the recovered colonies was tested for repair at each of six different sites. We observed that mammalian cells are capable of repairing single-stranded gaps and free single-stranded ends most efficiently. Regions containing twin loops were recognized, and one of the loops was excised. Portions of the molecules containing small single loops were also repaired. Markers which were 58 nucleotides apart were corepaired with nearly 100% efficiency, while markers which were 1,000 nucleotides or more apart were never corepaired. The mechanisms involved in heteroduplex repair in mammalian cells seem to be similar to those involved in repairing DNA lesions caused by physical and chemical agents.
Effects of topical ocular administration of high doses of human recombinant interferon alpha-2b and feline recombinant interferon omega on naturally occurring viral keratoconjunctivitis in cats (2013)

In cats with naturally occurring viral keratoconjunctivitis, bilateral ocular administration of high doses of FelFN or HulFN twice daily for 14 days did not improve clinical disease or virus shedding, compared with treatment with saline solution.
Six degree of freedom simulation of fluid payload projectiles using numerically computed fluid moments (1985)

A flight trajectory simulation method has been developed for calculating the six degree of freedom motion of fluid filled projectiles. Numerically calculated internal fluid moments and experimentally known aerodynamic forces and moments are coupled to the projectile motion. Comparisons of predicted results with flight test data of an M483 155mm artillery projectile with a highly viscous payload confirm the accuracy of the simulation. This simulation clearly shows that the flight instability is due to the growth of the nutation component of angular motion caused by the viscous effects of the fluid payload. This simulation procedure, when used in conjunction with the previously developed method for calculating internal fluid moments, allows the designer to examine the effects of various liquid payloads and container geometries on the dynamic behavior of flight vehicles.
The liquid‐crystalline derivatives of 3,6‐disubstituted cyclohex‐2‐enones (2003)

Abstract— A new synthetic approach for the preparation of various mesomorphic chiral and non‐chiral derivatives of cyclohexene, cyclohexane, phenylcyclohexane, or biphenyl with different combinations of cyclic, bridge, terminal fragments and lateral substituents is proposed and realized. Both nematic and smectic liquid‐crystalline compounds were prepared by the transformations of the corresponding 3,6‐disubstituted cyclohex‐2‐enones.
Synthesis of Fused Pyrimido[1,6-a]indolones via Rhodium(III)-Catalyzed Cascade Annulations (2020)

Abstract A novel method for the synthesis of fused pyrimido[1,6-a]-indolone derivatives by annulation of 2-alkynylaryl aldehydes/2-alkynyl­arylidene ketones with N-(pivaloyloxy)-1H-indole-1-carboxamide catalyzed by rhodium has been accomplished. The reaction proceeds through C–H activation based annulation with alkyne moiety followed by addition of nitrogen on to aldehyde/activated alkene to give the products in moderate to good yields. Highly fluorescent dipyrrinone analogues could be synthesized from the derived products.
Identification and Characterization of All-trans-retinol Dehydrogenase from Photoreceptor Outer Segments, the Visual Cycle Enzyme That Reduces All-trans-retinal to All-trans-retinol (2000)

Retinol dehydrogenase (RDH), the enzyme that catalyzes the reduction of all-trans-retinal to all-trans-retinol within the photoreceptor outer segment, was the first visual cycle enzymatic activity to be identified. Previous work has shown that this enzyme utilizes NADPH, shows a marked preference for all-trans-retinal over 11-cis-retinal, and is tightly associated with the outer segment membrane. This paper reports the identification of a novel member of the short chain dehydrogenase/reductase family, photoreceptor RDH (prRDH), using subtraction and normalization of retina cDNA, high throughput sequencing, and data base homology searches to detect retina-specific genes. Bovine and human prRDH are highly homologous and are most closely related to 17-beta-hydroxysteroid dehydrogenase 1. The enzymatic properties of recombinant bovine prRDH closely match those previously reported for RDH activity in crude bovine rod outer segment preparations. In situ hybridization and RNA blotting show that the PRRDH gene is expressed specifically in photoreceptor cells, and protein blotting and immunocytochemistry show that prRDH localizes exclusively to both rod and cone outer segments and that prRDH is tightly associated with outer segment membranes. Taken together, these data indicate that prRDH is the enzyme responsible for the reduction of all-trans-retinal to all-trans-retinol within the photoreceptor outer segment.
State of the Art in the Studies on Crotamine, a Cell Penetrating Peptide from South American Rattlesnake (2014)

Animal venoms comprise a naturally selected cocktail of bioactive peptides/proteins and other molecules, each of which playing a defined role thanks to the highly specific interactions with diverse molecular targets found in the prey. Research focused on isolation, structural, and functional characterizations of novel natural biologics (bioactive peptides/proteins from natural sources) has a long way to go through from the basic science to clinical applications. Herein, we overview the structural and functional characteristics of the myoneurotoxin crotamine, firstly isolated from the South American rattlesnake venom. Crotamine is the first venom peptide classified as a natural cell penetrating and antimicrobial peptide (CPP and AMP) with a more pronounced antifungal activity. In contrast to other known natural CPPs and AMPs, crotamine demonstrates a wide spectrum of biological activities with potential biotechnological and therapeutic values. More recent studies have demonstrated the selective in vitro anticancer activity of crotamine. In vivo, using a murine melanoma model, it was shown that crotamine delays tumor implantation, inhibits tumor cells proliferation, and also increases the survival of mice engrafted with subcutaneous melanoma. The structural and functional properties and also the possible biotechnological applications of minimized molecules derived from crotamine are also discussed.
Performance Evaluation of the ROSENET Network Emulation System (2009)

Network emulation has been widely used to aid in the development and evaluation of real-time applications. Traditional emulation tools based on a single node machine or a cluster of machines cannot satisfy today's requirements for testing real-time applications distributed over wide area networks. ROSENET (RemOte SErver-based Network EmulaTion) is a network emulation approach that exploits a remote high-fidelity simulation to provide scale and accuracy for a low-fidelity emulator serving a locally executing real-time application. This paper evaluates the performance of an implementation of ROSENET using the NIST Net emulator and GTNetS simulator. Experimental results examining end-to-end delay and loss show that ROSENET provides a promising approach to network emulation supporting accuracy and scale while meeting real-time constraints.
ENZYMATIC DEBRIDEMENT OF BURNS* (1951)

Altemeier, William A. M.D.; Coith, Robert M.D.; Culbertson, William M.D.; Tytell, Alfred Ph.D Author Information
Cytotoxic potential despite impaired activation pathways in T lymphocytes infiltrating nasopharyngeal carcinoma (1991)

Nasopharyngeal carcinoma (NPC) is an epithelial tumor consistently associated with EBV. The histological picture is characterized by a strikingly abundant lymphocytic infiltrate. Furthermore, the epithelial tumor cells present several immunological characteristics which suggest an important role for tumor-infiltrating lymphocytes (TIL) in the biology of this tumor. The present study reports the phenotypic and functional characterization of TIL from NPC obtained after enzymatic digestion of 15 NPC biopsies. Flow cytometric analysis of TIL suspensions indicated that most TIL were mature CD3+ T lymphocytes (mean = 60%) with a variable CD4/CD8 ratio. Most TIL were TCR alpha/beta-positive (mean = 55%) and only a few TCR gamma-delta-positive cells could be identified. A small percentage (mean = 9%) displayed an activated phenotype (CD25+, HLA class II+). Using limiting dilution analysis, we found that the average frequency of proliferative T-lymphocyte precursors (PTL-P) is lower among TIL (1/40) than in autologous (1/7) or normal PBL (1/1.4). Moreover, sorting experiments have shown that this defect is significantly more pronounced in the CD8+ than in the CD4+ TIL subset. Accordingly, the TCR and the CD2-mediated antigen-independent pathways of activation were impaired. Different types of cytotoxic precursor could be detected. These included lectin-dependent cell cytotoxicity (LDCC) and NK-like or lymphokine-activated killer (LAK) activity. Interestingly, some TIL from NPC were able to lyse an NPC tumor (C15) maintained in nude mice. Thus, despite impaired activation pathways, the cytolytic potential of proliferating TIL in NPC is preserved.
Suspended matter on the meridional section in the Laptev Sea in August-September 1991 (1999)

Suspended matter concentration along a meridian section from the Lena River delta to 78°N (~500 km) at ten stations from the surface to the bottom was studied with weight and optical (light attenuation index) techniques. At seven stations average residence time of suspended matter in surface waters was determined by the disequilibrium 234Th method. Average residence time of suspended matter in other depth intervals was calculated by regression dependence between the 234Th/238U ratio and suspended matter concentration. Differential and integral fluxes of suspended matter in the water column were also calculated. Nepheloid matter dominates in suspended matter composition in surface waters. Calculations indicate that, before being buried on the bottom, solid river run-off is resuspended 2.3 times (aver.). Redistribution of nepheloid suspended matter in the near-bottom layer results in formation of a strongly pronounced depocentre - an area of maximal discharge of solid river run-off within the Laptev Sea.