gebhart/patrepeval huggingface dataset:
Synthesis and Nucleophilic Substitution of Highly Chlorinated Arene (.eta.5-Pentamethylcyclopentadienyl)ruthenium .pi.-Complexes (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Nucleophilic Substitution of Highly Chlorinated Arene (.eta.5-Pentamethylcyclopentadienyl)ruthenium .pi.-ComplexesAlexa A. Dembek and Paul J. FaganCite this: Organometallics 1995, 14, 8, 3741–3745Publication Date (Print):August 1, 1995Publication History Published online1 May 2002Published inissue 1 August 1995https://pubs.acs.org/doi/10.1021/om00008a022https://doi.org/10.1021/om00008a022research-articleACS PublicationsRequest reuse permissionsArticle Views243Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Experimental analysis and simulation of flow through multi‐layer fiber reinforcements in liquid composite molding (1998)

Abstract Liquid composite molding (LCM) is a process in which a reactive fluid is injected into a closed mold cavity with preplaced reinforcement. Combined layers of different permeabilities are often used in LCM, which creates through thickness and inplane porosity and permeability variations. These inhomogeneities may influence the flow front profile in the thickness direction. To investigate the effect of the through thickness inhomogeneities, mold filling experiments were performed using preforms containing layers of two different fiber architectures. Aqueous corn syrup solutions were injected into a tempered glass mold containing the reinforcement stack. The progress of the flow front at various locations within the reinforcement was measured by an electrical conductivity technique based on the insertion of small wires between the reinforcement layers. Experimental data reveal the details of the flow front shape as the fluid penetrates the preform. Using these data, a model is proposed to calculate the overall in‐plane permeability of the preform. Numerical simulations of the flow front progression performed with the computer software RTMFLOT developed in our laboratory are compared to the experimental flow front for various stacking arrangements. Results show good agreement between simulations and experiments and demonstrate the capability of the software to simulate multi‐layer flow process.
Role of cerebellum in adaptive modification of reflex blinks. (1997)

We investigated the involvement of the cerebellar cortex in the adaptive modification of corneal reflex blinks and the regulation of normal trigeminal reflex blinks in rats. The ansiform Crus I region contained blink-related Purkinje cells that exhibited a complex spike 20.4 msec after a corneal stimulus and a burst of simple spike activity correlated with the termination of orbicularis oculi activity. This occurrence of the complex spike correlated with trigeminal sensory information associated with the blink-evoking stimulus, and the burst of simple spike activity correlated with sensory feedback about the occurrence of a blink. Inactivation of the inferior olive with lidocaine prevented all complex and significantly reduced simple spike modulation of blink-related Purkinje cells, but did not alter orbicularis oculi activity evoked by corneal stimulation. In contrast, both acute and chronic lesions of the cerebellar cortex containing blink-related Purkinje cells blocked adaptive increases in orbicularis oculi activity of the lid ipsilateral but not contralateral to the lesion. These data are consistent with the hypothesis that the cerebellum is part of a trigeminal reflex blink circuit. Changes in trigeminal signals produce modifications of the cerebellar cortex, which in turn, reinforce or stabilize modifications of brainstem blink circuits. When the trigeminal system does not attempt to alter the magnitude of trigeminal reflex blinks, cerebellar input has little or no effect on reflex blinks.
Organization of point contacts in neuronal growth cones (1999)

Growth cones from rat dorsal root ganglia plated on laminin contain integrin clusters over the entire growth cone surface, and growth cones make transient adhesions at sites called point contacts. We examined, by immunocytochemistry and confocal microscopy, the composition and distribution of point contacts in neuronal growth cones. Vinculin was concentrated in the central domain of growth cones and at the tips of filopodia. Vinculin was specifically associated with integrin clusters at the membrane-substrate interface and thus marked point contacts. The cytoskeletal proteins paxillin and talin colocalized with beta1 integrin in a subpopulation of clusters restricted to the central domain of the growth cone and to the tips of filopodia. The neuron-specific kinase, FAK+ also distributed with the vinculin-positive clusters. The Rho family proteins RhoA, RhoB, and Cdc42 were present in growth cones, and a few Rho clusters were colocalized with vinculin. Examination of proteins resistant to detergent extraction in PC12 cells confirmed the retention of beta1 integrin, paxillin, talin, and vinculin with the cytoskeleton. Moreover, we detected FAK+ and RhoA in the detergent-resistant cytoskeleton, supporting their distribution to point contacts. Our observations indicate that two types of integrin clusters are present in growth cones: those associated with vinculin at the cell substratum interface, and those not associated with vinculin. Point contacts are mature adhesion sites defined by the presence of both beta1 integrin and vinculin, and they are associated with signaling proteins.
[Mebendazole and intestinal nematodiasis in Gabon. Tolerance and efficacy in single and multiple doses]. (1982)

Mebendazole was given following the classical regimen i. e. a three days course with a single intake per day for patients suffering from intestinal nematodosias in Gabon. Efficiency which is remarkable on this three days basis proved to be insufficient however for ankylostomiasis with a single intake per day. Tolerance is excellent on any therapeutical regimen or with any type of survey considered.
Dynamics of hospitalizations in hemodialysis patients: results from a large US provider (2013)

Hospitalization rates increase sharply before death. Months before the first hospitalization changes in clinical and laboratory parameters ensued. These results suggest that models aiming at predicting hospitalization should include indicators capturing the dynamics of relevant parameters.
PGE2: a mediator of corneal endothelial wound repair in vitro (1994)

Maintenance of the integrity of the corneal endothelial monolayer is essential for corneal clarity. Aging, trauma, inflammation, and diseases, such as diabetes, can compromise monolayer integrity, resulting in corneal edema and loss of visual acuity. In adult humans, repair of the monolayer occurs mainly by two forms of cell movement: "migration," in which individual cells move from the wound edge to repopulate the defect, and "spreading," in which cells enlarge and flatten, causing movement of the monolayer as a sheet to cover the defect. Previous studies from this laboratory have shown that these two forms of movement can be pharmacologically separated. In the current studies, an established tissue culture model was used to determine the effect of prostaglandin E2 (PGE2) and of mediators of the adenosine 3',5'-cyclic monophosphate (cAMP) pathway on individual cell migration. Indomethacin, an inhibitor of prostaglandin synthesis, significantly decreased individual cell migration below levels obtained when wounds were exposed to culture medium alone. PGE2, but not PGF2 alpha, restored this response in a dose-dependent manner. In the presence of indomethacin, forskolin, a direct adenylate cyclase activator, stimulated individual cell migration. 2',5'-Dideoxyadenosine, an adenylate cyclase inhibitor, reversed the stimulatory effects of both forskolin and PGE2. Dibutyryl cAMP (DBcAMP) also stimulated individual cell migration in the presence of indomethacin, whereas, H89, a protein kinase A inhibitor, reversed both the DBcAMP and PGE2-induced effects. These results provide evidence that stimulation of the cAMP pathway enhances individual cell migration and that PGE2, acting via this pathway, may be an endogenous stimulator of this response during corneal endothelial wound repair.
Quantitative two-photon flow cytometry—in vitro and in vivo (2008)

Flow cytometry is a powerful technique for quantitative characterization of fluorescence in cells. Quantitation is achieved by ensuring a high degree of uniformity in the optical excitation and detection, generally by using a highly controlled flow. Two-photon excitation has the advantages that it enables simultaneous excitation of multiple dyes and achieves a very high SNR through simplified filtering and fluorescence background reduction. We demonstrate that two-photon excitation in conjunction with a targeted multidye labeling strategy enables quantitative flow cytometry even under conditions of nonuniform flow, such as may be encountered in simple capillary flow or in vivo. By matching the excitation volume to the size of a cell, single-cell detection is ensured. Labeling cells with targeted nanoparticles containing multiple fluorophores enables normalization of the fluorescence signal and thus quantitative measurements under nonuniform excitation. Flow cytometry using two-photon excitation is demonstrated for detection and differentiation of particles and cells both in vitro in a glass capillary and in vivo in the blood stream of live mice. The technique also enables us to monitor the fluorescent dye labeling dynamics in vivo. In addition, we present a unique two-beam scanning method to conduct cell size measurement in nonuniform flow.
A high-quality genome of Eragrostis curvula grass provides insights into Poaceae evolution and supports new strategies to enhance forage quality (2019)

The Poaceae constitute a taxon of flowering plants (grasses) that cover almost all Earth's inhabitable range and comprises some of the genera most commonly used for human and animal nutrition. Many of these crops have been sequenced, like rice, Brachypodium, maize and, more recently, wheat. Some important members are still considered orphan crops, lacking a sequenced genome, but having important traits that make them attractive for sequencing. Among these traits is apomixis, clonal reproduction by seeds, present in some members of the Poaceae like Eragrostis curvula. A de novo, high-quality genome assembly and annotation for E. curvula have been obtained by sequencing 602 Mb of a diploid genotype using a strategy that combined long-read length sequencing with chromosome conformation capture. The scaffold N50 for this assembly was 43.41 Mb and the annotation yielded 56,469 genes. The availability of this genome assembly has allowed us to identify regions associated with forage quality and to develop strategies to sequence and assemble the complex tetraploid genotypes which harbor the apomixis control region(s). Understanding and subsequently manipulating the genetic drivers underlying apomixis could revolutionize agriculture.
Interferenceless Recording of Coherent Holograms using Coded Phase Apertures (2020)

Three-dimensional (3D) imaging of coherently illuminated scenes without two-beam interference is demonstrated. The coded aperture imaging system records digital holograms of 3D scenes without interference with a reference wave and by only two camera shots.
Alterations in renal endothelin-1 production in the spontaneously hypertensive rat. (1992)

Endothelin-1 inhibits sodium and water transport systems in the inner medullary collecting duct. Endothelin-1 levels are reduced in the medulla of spontaneously hypertensive rats (SHR), raising the possibility that decreased inner medullary collecting duct production of endothelin-1 could contribute to inappropriate sodium and water retention. In the current study, immunoreactive endothelin-1 was measured in the urine, blood, and eluates from cortex and outer and inner medulla of SHR before (age 3-4 weeks) and after (age 8-9 weeks) the development of hypertension and in age-matched Wistar-Kyoto (WKY) controls. There was no difference in endothelin-1 levels between prehypertensive SHR and WKY rats. In contrast, 8-9-week-old SHR had significantly reduced endothelin-1 in the urine and outer and inner medulla, but not in the cortex or serum compared with those of WKY controls. Furthermore, inner medullary collecting duct cells from 8-9-week-old SHR, either acutely isolated or cultured, released less endothelin-1 than did those from WKY rats. Finally, the level of endothelin-1 messenger RNA was only reduced in the inner medulla and in inner medullary collecting duct cells from 8-9-week-old SHR. In summary, renal medullary, and in particular terminal collecting duct, endothelin-1 production is reduced in SHR only after the development of hypertension. Such decreases in inner medullary collecting duct endothelin-1 production may contribute to the hypertensive state in SHR.
New Amebacides. V.1 The Preparation of Some N-Alkoxyalkyl-N-benzyldichloroacetamides (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Amebacides. V.1 The Preparation of Some N-Alkoxyalkyl-N-benzyldichloroacetamidesAlexander R. Surrey, Arthur J. Olivet, Stanley O. Winthrop, and George Y. LesherCite this: J. Am. Chem. Soc. 1956, 78, 15, 3834–3836Publication Date (Print):August 1, 1956Publication History Published online1 May 2002Published inissue 1 August 1956https://pubs.acs.org/doi/10.1021/ja01596a075https://doi.org/10.1021/ja01596a075research-articleACS PublicationsRequest reuse permissionsArticle Views53Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Process related yield risk mitigation with in-design pattern replacement for system ICs manufactured at advanced technology nodes (2020)

Process and reliability risks have become critically important during mass production at advanced technology nodes even with Extreme Ultraviolet Lithography (EUV) illumination. In this work, we propose a design-for-manufacturability solution using a set of new rules to detect high risk design layout patterns. The proposed methods improve design margins while avoiding area overhead and complex design restrictions. In addition, the proposed method introduces an in-design pattern replacement with automatically generated fixing hints to improve all matched locations with identified patterns.
Conformational transitions in DNA polymerase I revealed by single-molecule FRET (2009)

The remarkable fidelity of most DNA polymerases depends on a series of early steps in the reaction pathway which allow the selection of the correct nucleotide substrate, while excluding all incorrect ones, before the enzyme is committed to the chemical step of nucleotide incorporation. The conformational transitions that are involved in these early steps are detectable with a variety of fluorescence assays and include the fingers-closing transition that has been characterized in structural studies. Using DNA polymerase I (Klenow fragment) labeled with both donor and acceptor fluorophores, we have employed single-molecule fluorescence resonance energy transfer to study the polymerase conformational transitions that precede nucleotide addition. Our experiments clearly distinguish the open and closed conformations that predominate in Pol-DNA and Pol-DNA-dNTP complexes, respectively. By contrast, the unliganded polymerase shows a broad distribution of FRET values, indicating a high degree of conformational flexibility in the protein in the absence of its substrates; such flexibility was not anticipated on the basis of the available crystallographic structures. Real-time observation of conformational dynamics showed that most of the unliganded polymerase molecules sample the open and closed conformations in the millisecond timescale. Ternary complexes formed in the presence of mismatched dNTPs or complementary ribonucleotides show unique FRET species, which we suggest are relevant to kinetic checkpoints that discriminate against these incorrect substrates.
Biosynthesis of the Isoprenoid Moieties of Furanonaphthoquinone I and Endophenazine A in Streptomyces cinnamonensis DSM 1042 (2007)

Streptomyces cinnamonensis DSM 1042 produces the polyketide-isoprenoid compound furanonaphthoquinone I (FNQ I) and isoprenylated phenazines, predominantly endophenazine A. However, the recently identified biosynthetic gene cluster for these compounds only contains a single gene for a mevalonate pathway enzyme, that is, a putative mevalonate kinase gene. This is in strong contrast to all Streptomyces strains examined so far, where all six genes encoding the mevalonate pathway enzymes are clustered in a single operon of 6.8 kb and, thus, raised the question about the biosynthetic origin of the isoprenoid moieties of FNQ I and endophenazine A. In this study, we investigated the incorporation of [13C2]acetate and [2-13C]glycerol into FNQ I and endophenazine A. The results unequivocally prove that the isoprenoid building blocks of both compounds are predominantly formed via the mevalonate pathway (approximately 80%) but that the MEP pathway (approximately 20%) contributes to the biosynthesis of these molecules, too. In actinomycetes, this is the first experimentally proven example of the utilization of both biosynthetic routes for the formation of one single secondary metabolite. The incorporation pattern of [2-13C]glycerol was consistent with a "reverse" prenyl transfer, that is, with the formation of a C-C bond from C-3 of GPP to the polyketide nucleus of FNQ I.
A compact optical design for Bragg reflections near backscattering (2001)

A very compact in-line X-ray optical design is presented which is optimized for use with Bragg reflections close to backscattering (Bragg angles near 90°). The essential idea is to use a low-order Bragg reflection to couple the X-ray beam into a small channel-cut backscattering crystal. The design is demonstrated in an almost theoretically performing high-resolution monochromator providing 2 × 108 photons s−1 in a 0.52 meV bandwidth at 25.65 keV. The monochromator is used to measure inelastic nuclear scattering from phonons in 161Dy-containing samples.
Controllable optical quality factor in a two-ring-two-bus structure (2021)

Dynamically tuning Q factor in optical resonators is now more feasible in information processing applications, such as light storage and wavelength conversion. This paper proposed and demonstrated a new dynamically tuning Q approach that is realized in coupled two rings structure. One resonator uses an add-drop configuration, coupled to another single micro-ring. The heating causes an increase in the refractive index, which in turn causes resonant wavelength redshift. This shift is a switch to control the coupling between the two rings, to tune the linewidth of light confined in the ring, equivalent to tune the quality factor. And we adjust the detuning and coupling state between the two rings to control the light coupled into the resonator. The Q factor decreases from 56,525 to 16,450 in the transmission spectrum with the different heater power. In this way, we successfully realized a modulation of the Q from high to low states in the structure. Besides, the original coupling spacing between the two rings also has an influence on the Q factor. The larger the coupling spacing, the higher the Q factor. In addition, we also study the changes of phase and delay time in the tunable process. The results show that the change of fast light (high Q) and slow light (low Q) can be realized at the same time, which would enable applications for on-chip adjustable time delay, fast/slow light and light storage.
Photocatalytic antibacterial performance of TiO2 and Ag-doped TiO2 against S. aureus. P. aeruginosa and E. coli (2013)

This paper reports the structural and optical properties and comparative photocatalytic activity of TiO2 and Ag-doped TiO2 nanoparticles against different bacterial strains under visible-light irradiation. The TiO2 and Ag-doped TiO2 photocatalysts were synthesized by acid catalyzed sol-gel technique and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), UV-vis spectroscopy and photoluminescence (PL). The XRD pattern revealed that the annealed sample of TiO2 has both anatase and rutile phases while only an anatase phase was found in Ag-doped TiO2 nanoparticles. The decreased band-gap energy of Ag-doped TiO2 nanoparticles in comparison to TiO2 nanoparticles was investigated by UV-vis spectroscopy. The rate of recombination and transfer behaviour of the photoexcited electron-hole pairs in the semiconductors was recorded by photoluminescence. The antimicrobial activity of TiO2 and Ag-doped TiO2 nanoparticles (3% and 7%) was investigated against both gram positive (Staphylococcus aureus) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacteria. As a result, the viability of all three microorganisms was reduced to zero at 60 mg/30 mL culture in the case of both (3% and 7% doping) concentrations of Ag-doped TiO2 nanoparticles. Annealed TiO2 showed zero viability at 80 mg/30 mL whereas doped Ag-TiO2 7% showed zero viability at 40 mg/30 mL culture in the case of P. aeruginosa only.