gebhart/patrepeval huggingface dataset:
Micromethodology for measuring ATPase activity in renal tubules: mineralocorticoid influence (1984)

The ATPase activity of rabbit isolated renal tubule segments was measured using a microtechnique in which the hydrolysis of ATP was enzymatically coupled to the appearance of an alkali-converted, highly fluorescent form of nicotinamide adenine dinucleotide. The methods are simple, reproducible, and have a high sensitivity in which picomole quantities of hydrolyzed ATP can readily be measured. Several methods for permeabilizing the cell membranes for measurement of Na+-K+-ATPase activity were evaluated, including osmotic (distilled water or 300 mM imidazole) and temperature (freezing) shock and addition of the nonionic detergent octylglucoside. An octylglucoside concentration of 0.5% was found to cause a maximum activation of the Na+-K+-ATPase and was comparable with that observed when tubules were permeabilized by exposure to distilled water and freezing. Incubation of tubules in 300 mM imidazole was less effective in permeabilizing the cell membranes. In all subsequent studies, the cells were permeabilized by exposure to distilled water and freezing as done by others. The methods were used to assay for the basal levels of Na+-K+-ATPase in the superficial proximal convoluted tubule, the superficial proximal straight tubule, and the cortical collecting tubule and were found to average 44.9 +/- 6.3, 26.4 +/- 2.4, and 11.8 +/- 2.2 pmol ADP X mm-1 X min-1, respectively. Furthermore, elevation of plasma mineralocorticoids by daily injections of deoxycorticosterone acetate (2 mg X kg-1 X day-1) for 4-15 days caused a doubling in the Na+-K+-ATPase activity of the cortical collecting duct, confirming the results of others. The methods presented can easily be adapted for microanalysis of other ATPases.
A media synchronization experiment on continuous media transmission in Bluetooth LAN access (2002)

This paper assesses the media synchronization quality in a Bluetooth LAN access system by experiment. We consider a situation where a server transfers stored video and audio streams to a terminal with point-to-point communication. Both FEC and ARQ are adopted as error control schemes. The slide control scheme is used for media synchronization control. We examine how the interference from DSSS systems affects the quality. For simplicity of experiment, we suppose that the Bluetooth terminal does not move while the media streams are transferred, i.e., fading does not occur. In the experiment, we evaluated several performance measures such as mean square error of intra-stream synchronization. We then found that delay jitters caused by retransmission of DM1 packets disturbed temporal relations of the streams. We also observed that the media synchronization control is effective in compensating for the disturbance.
Risk of Early Surgery for Crohn's Disease: Implications for Early Treatment Strategies (2003)

The rate of surgery is high in the first 3 yr after diagnosis of Crohn's disease, particularly in the first 6 months. These results suggest that improved risk stratification and potent therapies with rapid onset of action are needed to modify the natural history of Crohn's disease.
Cryo-EM structures of Doravirine and Rilpivirine with HIV-1 Reverse Transcriptase/DNA Aptamer – Nonnucleoside Inhibitor Resistance by E138K and M184I Mutations (2022)

Abstract Structures trapping a verity of functional and conformational states of HIV-1 reverse transcriptase (RT) have been determined by X-ray crystallography. These structures have played important roles in understanding the mechanisms of catalysis, inhibition and drug resistance, and in driving drug design. However, structures of several desired complexes of RT could not be obtained even after many crystallization or crystal soaking experiments. The ternary complexes of doravirine and rilpivirine with RT/DNA are such examples. Structural study of HIV-1 RT by single-particle cryo-EM has been challenging due to the enzyme’s relatively smaller size and higher flexibility. We optimized a protocol for rapid structure determination of RT complexes by cryo-EM and determined six structures of wild-type and E138K/M184I mutant RT/DNA in complexes with the nonnucleoside inhibitors rilpivirine, doravirine, and nevirapine. RT/DNA/rilpivirine and RT/DNA/doravirine complexes have structural differences between them and from the representative conformation of RT/DNA/nevirapine (or efavirenz); the primer grip in the RT/DNA/doravirine and the YMDD motif in the RT/DNA/rilpivirine complexes have large shifts. The DNA primer 3’-end in the doravirine-bound structure is positioned at the active site, but the complex is in a non-productive state. In the mutant RT/DNA/rilpivirine structure, I184 is stacked with the DNA such that their relative positioning can influence rilpivirine in the pocket. Simultaneously, E138K mutation widens the NNRTI-binding pocket entrance, potentially contributing to a faster rate of rilpivirine dissociation by E138K/M184I mutant RT, as reported by earlier kinetic studies. These structural differences have implications for drug design and for understanding molecular mechanisms of drug resistance.
MuSICa: THE MULTI-SLIT IMAGE SLICER FOR THE EST SPECTROGRAPH (2013)

Integral field spectroscopy (IFS) is a technique that allows one to obtain the spectra of all the points of a bidimensional field of view simultaneously. It is being applied to the new generation of the largest night-time telescopes but it is also an innovative technique for solar physics. This paper presents the design of a new image slicer, MuSICa (Multi-Slit Image slicer based on collimator-Camera), for the integral field spectrograph of the 4-m aperture European Solar Telescope (EST). MuSICa is a multi-slit image slicer that decomposes an 80 arcsec 2 field of view into slices of 50 μm and reorganizes it into eight slits of 0.05 arcsec width × 200 arcsec length. It is a telecentric system with an optical quality at diffraction limit compatible with the two modes of operation of the spectrograph: spectroscopic and spectro-polarimetric. This paper shows the requirements, technical characteristics and layout of MuSICa, as well as other studied design options.
Applying pipelining techniques to microprocessors (1984)

Pipelining techniques are used in the Intel iAPX 286. High performance with full protection using normal speed memories require pipelining techniques. Pipelining gets the most done in each processor clock cycle. The internal pipelining is then exported to the memory bus to allow usage of slow memory devices.
Digital micromirror device for optical scanning applications (2007)

Recent mechanical and nonmechanical optical scanning devices do not meet the fast scanning requirements for contemporary and emerging applications and can only steer optical beams over relatively narrow angles. A variety of important applications require fast optical scanning devices that can steer laser beams rapidly to an arbitrary location and with no moving parts. We introduce a new optical scanning technique that can be used to collimate and steer optical beams for precision alignment in either 2-D free-space optical (FSO) communications links or image scanners. This nonmechanical technique is capable of rapidly redirecting the optical beams to arbitrary locations without greatly sacrificing other parameters such as aperture size, efficiency, and scanning range. A digital micromirror device (DMD) beam-steering system was successfully demonstrated and exhibited better performance results when compared with other available systems.
Hyperbranched Polymer-Based Electrolyte for Lithium Polymer Batteries (2005)

1Introduction Solid polymer electrolytes have attracted much attention as electrolyte materials for all solid-state recharge able lithium batteries, and poly ( ethylene oxide) ( PEO)-based polymer electrolytes are among the most intensively studied systems[1-3]. Hyperbranched polymers have unique properties such as completely amorphous, highly soluble in common organic solvent and processible because of the highly branched nature[4,5].
Using Intake Biomarkers to Evaluate the Extent of Dietary Misreporting in a Large Sample of Adults: The OPEN Study (2003)

This paper describes the Observing Protein and Energy Nutrition (OPEN) Study, conducted from September 1999 to March 2000. The purpose of the study was to assess dietary measurement error using two self-reported dietary instruments-the food frequency questionnaire (FFQ) and the 24-hour dietary recall (24HR)-and unbiased biomarkers of energy and protein intakes: doubly labeled water and urinary nitrogen. Participants were 484 men and women aged 40-69 years from Montgomery County, Maryland. Nine percent of men and 7% of women were defined as underreporters of both energy and protein intake on 24HRs; for FFQs, the comparable values were 35% for men and 23% for women. On average, men underreported energy intake compared with total energy expenditure by 12-14% on 24HRs and 31-36% on FFQs and underreported protein intake compared with a protein biomarker by 11-12% on 24HRs and 30-34% on FFQs. Women underreported energy intake on 24HRs by 16-20% and on FFQs by 34-38% and underreported protein intake by 11-15% on 24HRs and 27-32% on FFQs. There was little underreporting of the percentage of energy from protein for men or women. These findings have important implications for nutritional epidemiology and dietary surveillance.
Synthesis of 5,6,17,18-Bisdehydrotetrathia[24]annulene[2.2.2.2]. A Strained Planar Annulene Devoid of Peripheral Conjugation (1995)

Abstract 5,6,17,18-Bisdehydrotetrathia[24]annulene[2.2.2.2], synthesized through a sequence of the McMurry coupling of 1,1-dichloro-2,2-di(5-formyl-2-thienyl)ethene and a novel double Fritsch-Buttenberg-Wiechell rearrangement, has a planar X-ray structure with bent triple bonds and short S-S contacts but shows little sign of peripheral conjugation.
Iridium-complex modified CdSe/ZnS quantum dots; a conceptual design for bifunctionality toward imaging and photosensitization (2006)

We report the design and synthesis of Ir-complex functionalized CdSe/ZnS quantum dots (QDs), in which the QD plays a key role in imaging, while the Ir-complex acts as a sensitizer to produce singlet oxygen; this conceptual design presents a novel scheme in both bio-imaging and photodynamic therapy.
New Quinovic Acid Glycosides from Uncaria tomentosa (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Quinovic Acid Glycosides from Uncaria tomentosaRiccardo Cerri, Rita Aquino, Francesco de Simone, and Cosimo PizzaCite this: J. Nat. Prod. 1988, 51, 2, 257–261Publication Date (Print):March 1, 1988Publication History Published online1 July 2004Published inissue 1 March 1988https://pubs.acs.org/doi/10.1021/np50056a010https://doi.org/10.1021/np50056a010research-articleACS PublicationsRequest reuse permissionsArticle Views364Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Immunogenicity of Therapeutic Proteins (2010)

Abstract The introduction of recombinant DNA techniques has made possible to produce proteins on a large scale. These protein therapeutics are potentially immunogenic, inducing antibodies in patients after a certain period of treatment. This article gives an overview of the current knowledge about the immunogenicity of therapeutic proteins.
Understanding and modelling the mixed lubrication regime: summary of achievements and perspectives of industrial applications (2001)

The works described previously, and summarized here, have already been applied under various forms, to be described hereafter: explanation of industrial observations, knowledge models, new ideas for lubricant formulation, which now have to be tested. Of course, not all the needs of industry have been fulfilled, so that perspectives for further research are highlighted.
BENEFÍCIOS DA SUSPENSÃO DE CÉLULAS VEGETAIS PARA OS FUNGOS MICORRÍZICOS VESÍCULO-ARBUSCULARES IN VITRO. (1990)

The effects of different culture medium of plant cells (B5, LS, MS, 5H and White) were evaluated iii the presence or absence of Pueraria Puera ria phaseoloides (Roxb.) Benth cells upon germiriation and growth of mycelium of Seuteliospora heterogama. Nondiiuted MS medium extended the viabiity period of cells and at ten times (0,1X MS) dilution and addition of cells from Pueraria hypocotyls promoted the greatest benefit to germination and ramitication of the mycelium of Gigaspora gigantea, Gigaspora margarita and Scutellospora heterogama. Despite the significant myceium growth, colonization or sporulation by the VAM fungi in the culture medium containing plant ceus were nol observed.
Emergent Management of APC-2 Pelvic Ring Injuries With an Anteriorly Placed C-Clamp (2009)

The pelvic C-clamp can be placed anteriorly as a part of the early management of APC-2 pelvic fractures with a short application time in a variety of patient care areas.
The ethical use of fetal tissue for transplantation and research (1993)

The exercise of organ and tissue transplantation is a common occurrence in our society. Expectations of future advances in this arena are raising many ethical, moral and public-policy questions. The scientific data base supporting the usefulness of fetal tissue to repair or reverse disease is modest, and uses are speculative. Should fetal tissue transplantation research proceed and, if so, under what conditions? This paper examines the issues related to the use and procurement of fetal tissue for research and transplantation.
Does Plate Fixation Prevent Disc Degeneration After a Lateral Anulus Tear? (1994)

Disc degeneration was not prevented by this method of plate fixation, despite similar but limited healing of the outer anulus tear in both plated and nonplated levels.
Low-Energy Ion Transport Between Two Insulating Parallel Plates (2021)

Abstract Experimental data are presented for low-energy singly charged ion transport between two insulating parallel plates. Using a beam intensity of approximately 20 pA, measurements of the incoming and transmitted beams provide quantitative temporal information about the charge deposited on the plates and the guiding probability. Using a smaller beam intensity (~1 pA) plate charging and discharging properties were studied as a function of time. These data imply that both the charge deposition and decay along the surface and through the bulk need to be modeled as acting independently rather than as a combined weighted average. A further reduction of beam intensity to ~25 fA allowed temporal imaging studies of the positions and intensities of the guided beam plus two bypass beams to be performed. Because of the parallel plate geometry, SIMION software was used to simulate trajectories of the guided and bypass beams. This provides information about the amount and location of deposited charge and, as a function of charge patch voltage, the probability of beam guiding and how much the bypass beams are deflected. Information about the electric fields which provides insights into the relative charge decay via the surface and bulk is also obtained. An equivalent electric circuit model of the parallel plates is used to associate the deposited charge with the patch voltage. To achieve internal consistency between the various sets of experimental data and the SIMION information, the deposited charge is implied to be distributed primarily on the inner surface of the plates, transverse to the beam direction, rather than being distributed throughout the entire plate.
EBSD Characterization of Specific Microstructures in RE-BCO Superconductors (2018)

Using the electron backscatter diffraction (EBSD) technique, we analyze in this contribution the microstructure of YBa <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> Cu <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> (YBCO) thick films with embedded a-axis oriented grains. The YBCO films were prepared by liquid phase epitaxy in air using NdGaO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> substrates. Using this technique, a/c-grain boundaries (GBs) with a well-defined facet of the YBCO film characterized by a single crystalline nature are obtained. This type of GB is very important for the basic physics of GBs in high-T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> superconductor materials, and will contribute to a better understanding of GBs also in bulk materials. The application of EBSD to the analysis of a/c-GBs in YBCO requires a high Kikuchi pattern quality (i.e., image quality) to enable a proper distinction of the orientation, as the a-axis corresponds to about one-third of the c-axis, which can cause a misinterpretation of the resulting data. Having solved this problem, the EBSD-based determination of the GB misorientation angles directly proof the specific grain orientation achieved in the film growth.
Network delivery of live events in a Digital Cinema scenario (2008)

The goal of this paper is to present a system able to support the network delivery of live events in an expected, future Digital Cinema scenario. This service can consume a large amount of network bandwidth, due to the large volume of transmitted data and to the number of receivers, thus multicast transmission proves to be very useful. Consequently, a key issue of the system is the request routing algorithm, the goal of which is to optimise the QoS-guaranteed delivery of live streams in the backbone, each one towards a set of theatres. We consider the Multi Protocol Label Switching, which has emerged as an elegant solution to meet traffic engineering and resource reservation requirements, and focus on the overall request routing procedure, the mathematical modelling of the problem, and relevant solving algorithms. We present the comparative performance evaluation of these algorithms by means of an extensive simulation campaign performed with the OMNET++ simulation platform.
Three-dimensionally gradient harmonic structure design: an integrated approach for high performance structural materials (2016)

This paper presents an overview on the peculiar microstructural design, called ‘harmonic structure’ (HS), for improved mechanical performance of structural materials. A well designed powder metallurgy processing approach has been developed to create a unique three-dimensionally gradient HS with controlled bimodal grain size distribution in metals and alloys. The bulk materials with HS exhibited considerably higher strength and improved toughness as compared to coarse-grained structures. The unique HS design promotes uniformity of deformation by avoiding strain localization during plastic deformation. A possible mechanism of deformation behavior of HS has also been proposed based on the available experimental results.
An Improved Perturbation Theory for Optical Solitons Near the Zero-Dispersion Point (1996)

In communication lines operated near the zero-dispersion point, the third-order dispersion (TOD) can strongly affect the pulse dynamics. Hence, it is necessary to develop proper theoretical tools for this case. Besides the numerical methods, a perturbation theory that can take into account the bandwidth-limited amplification (BLA) and nonlinear gain (NLG) or losses is required. The latter perturbations can considerably reduce soliton interactions. With regard to a combined effect of TOD and BLA, it was shown [1] that, e.g., a decay of a two-soliton bound state caused by TOD can be prevented by BLA. A new idea put forward in [2, 3] was that BLA which compensates for the linear and nonlinear losses can, simultaneously, absorb the resonance radiation emitted under the action of TOD, thus lending the soliton a much better stability. This effect has been experimentally observed in [4]. For large values of TOD, however, the soliton was obtained numerically [3] in a state with a conspicuous velocity, which differed from that predicted by the usual perturbation approach [5] by a factor of 2.
Metrics-only Training Neural Network for Switching among an Array of Feedback Controllers for Bicycle Model Navigation (2022)

The paper proposes a novel training approach for a neural network to perform switching among an array of computationally generated stochastic optimal feedback controllers. The training is based on the outputs of off-line computed lookup-table metric (LTM) values that store information about individual controller performances. Our study is based on a problem of bicycle kinematic model navigation through a sequence of gates and a more traditional approach to the training is based on kinematic variables (KVs) describing the bicycle-gate relative position. We compare the LTM and KV based training approaches to the navigation problem and find that the LTM training has a faster convergence with less variations than the KV based training. Our results include numerical simulations illustrating the work of the LTM trained neural network switching policy.
DCM detection for on-line adaption of control parameters for totem-pole PFC rectifier (2015)

This paper presents a current control method for totem-pole PFC-rectifiers which avoids the need for expensive sensing circuits. The design makes use of only one switch current as current feedback signal. The control method is based on a detection algorithm for conduction mode detection in mixed conduction mode (MCM) operation. Using a DSP the mode detection is calculated on-line and based on the detection state the control algorithm adapts the control parameters in a few DSP cycles. This simple current assessment enables calculation of the a control-to-inductor model for discontinuous operation mode (DCM) to modify the current loop characteristic. The controller design was performed offline and is based on the nonlinear average current mode control. Control characteristics and performance of the new method are verified by measurement results on an interleaved totem-pole PFC-rectifier for on-board charger applications with 3600 W output power. Conducted efficiency and power factor measurements illustrate the excellent performance of the converter.