gebhart/patrepeval huggingface dataset:
Role of Vacuolar H+-ATPase in Interferon-Induced Inhibition of Viral Glycoprotein Transport (1999)

We have shown previously that interferon-beta (IFN-beta) induces the alkalinization of trans-Golgi network (TGN) and inhibits the transport of G protein of vesicular stomatitis virus (VSV) in L(B) cells and gD protein of herpes simplex virus (HSV-1) in LMtk- cells transfected with gD cDNA. The vacuolar H(+)-ATPase (V-ATPase) is responsible for maintaining pH in TGN, and V-ATPase-mediated acidification is required for normal transport of proteins. To examine whether alkalinization caused by IFN is mediated through V-ATPase, the activity of V-ATPase was determined in IFN-treated cells by coupling ATP hydrolysis to NADH oxidation. Bafilomycin (Baf) was used as positive control, as it specifically inhibits V-ATPase. The activity of V-ATPase was reduced in IFN-treated or Baf-treated cells compared with untreated cells. Doses of IFN-beta or Baf that neither alter pHi nor inhibit the transport of viral glycoproteins concomitantly inhibited the transport of G and gD proteins in TGN, as demonstrated by indirect immunofluorescence studies, and raised the pH of TGN as demonstrated by a decrease in the uptake of DAMP. Further, the effect of Baf on IFN-induced antiviral activity against VSV was examined to correlate the biologic significance of these findings. Data showed that Baf significantly enhances (5-50-fold) the IFN-induced antiviral activity as demonstrated by viral titers from supernatants. These findings suggest that the inhibition of transport of G and gD proteins by IFN-beta, may be related to the inhibition of V-ATPase-mediated acidification of TGN.
Bad-Data Sequence Detection for Power System State Estimation via ICA-GAN. (2020)

A deep learning approach to the detection of bad-data sequences in power systems is proposed. The bad-data model is nonparametric that includes arbitrary natural and adversarial data anomalies. No historical samples of data anomaly are assumed. The probability distribution of data in anomaly-free system operations is also non-parametric, unknown, but with historical training samples. A uniformity test is proposed based on a generative adversarial network (GAN) that extracts independent components of the measurement sequence via independent component analysis (ICA). Referred to as ICA-GAN, the developed approach to bad-data sequence detection can be applied at the individual sensor level or jointly at the system level. Numerical results demonstrate significant improvement over the state-of-the-art solutions for a variety of bad-data cases using PMU measurements from the EPFL smart grid testbed and that from the synthetic Northern Texas grid.
Simplex method in problems of light-beam phase control (1995)

The possibility of the application of the simplex method to problems of wave-front control for light beams propagating in a nonlinear medium is investigated. A numerical analysis of simplex-method effectiveness in comparison with the gradient procedure of hill climbing is carried out. The regimes of stationary and nonstationary wind refraction are considered. The simplest optimization of the simplex size and the control basis is done.
Study of high-speed distance relay using microcomputer (1977)

This paper deals with a study of a high-speed distance relay using a microcomputer. Operating task, performance and hardware system configuration of the microcomputer and operating characteristics of the high-speed distance relay are discussed. Achievement of rapid operating time and assurance of reliable operation of a relay system are inconsistent with each other, and therefore consideration has been made on the structure of the operating task of the microcomputer to compromise these two problems. The hardware system of the microcomputer has been formed so as to show the full capability of the high-speed processing. Through various characteristic tests, it has been demonstrated that a prototype relay manufactured under the above consideration has satisfactory characteristics as a high-speed distance relay.
117 Minimal Scarring after Grafting a Novel Autologous Self-assembly Skin Substitute on Burn Patients (2018)

One of the main complications for patients surviving major burn injuries are contractures and hypertrophic scars. Since the dermis is responsible for the mechanical properties of the skin, we have designed a skin substitute comprising a dermis in addition to an epidermis and evaluated this autologous self-assembly skin substitute (SASS), produced by a tissue engineering approach. The dermis comprised fibroblasts secreting an endogenous extracellular matrix, without any exogenous scaffold. Patients were recruited through Health Canada’s Special Access Program, which allowed extensive burn injuries to be acutely treated with SASSs. The SASSs were grafted on debrided full-thickness wounds according to the same protocol used for autografts. The graft take and the persistence of the SASS’s epithelium overtime were evaluated. Comparative assessment of scar quality for the SASSs, autographs (AGs) and uninjured skin using the Cutometer®, Mexameter® and DermaScan C® devices were performed on a subset of patients. SASS was used as autologous grafts of 14 severely burned patients for deep-partial thickness and full thickness burn wound coverage. The mean of the total surface area grafted was 2,321 cm2 [range 420–6 925]. The median follow-up is 2.3 years [range 1 month-8 years]. One week after grafting, on average 98% [range 85–100%] graft take was obtained. No significant contraction was observed in vivo. SASSs promoted a particularly good healing process and ensuing suppleness. Minimal hypertrophic scars were only observed between the SASSs. The integrity of the transplanted SASSs persisted over time with no defect in epidermal regeneration and no significant contractures. The SASSs were not as pigmented as normal skin, despite some concentrated pigmentation spots that can increase in number and area with time depending on the pateint’s Fitzpatrick skin phototype. Sites grafted with SASSs were comparable to adjacent split-thickness AGs and uninjured skin regarding erythema (SASS site: 292.6 ± 88.5U vs AG: 315.75 ± 61.22U vs. uninjured skin: 258.8 ± 21.5U; p>0.05; N=4 to 8), elasticity (SASS site: 0.87 ± 0.40mm vs AG: 0.90 ± 0.37mm vs. uninjured skin: 1.31 ± 0.72 mm; p>0.05), skin thickness (SASS site: 1.68 ± 0.64mm vs. AG: 1.85 ± 0.45mm vs. uninjured skin: 1.85 ± 0.45 mm; p>0.05) and microscopic features. After grafting, the SASS has very good functional characteristics: minimal contraction and hypertrophic scar as well as long-term durability and tissue regeneration. The SASS is a promising skin substitute for grafting full-thickness skin injuries.
Novel Hemocompatible Imine Compounds as Alternatives for Antimicrobial Therapy in Pharmaceutical Application (2020)

The aim of this study was to synthesize, characterize, and evaluate neoteric imine compounds for antimicrobial activity and hemocompatibility. Four compounds were synthesized using 3-thiophene carboxaldehyde, ethanol, amine, and acetic acid. The compounds were characterized using nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD). A solubility study was conducted with various solvents and surfactants at 40 °C. An in vitro antimicrobial assay was performed against bacterial and fungal strains to determine the zone of inhibition and minimum inhibitory concentrations. Finally, an in vitro hemolysis study was conducted using rat erythrocytes. The structure of the compounds was confirmed by NMR, FTIR corroborated their functional group attributes, DSC determined their enthalpies of fusion and fusion temperatures, and PXRD confirmed their crystalline nature. These compounds were water-insoluble but soluble in chloroform, with a maximum solubility of ~80 mg/mL. The antimicrobial assay suggested that two of the products exerted potent activities against C. albicans and several bacterial strains. Finally, hemolysis analysis excluded the possibility of hemolysis at the assessed concentrations. In conclusion, two of the novel imine compounds showed promise as antimicrobial agents to control local and systemic microbial infections in a suitable dosage form.
Performance limitation of fiber optic methane sensors due to interference effects (1996)

We model interference effects in fiber optic methane sensors and predict the influence on the sensitivity. System sensitivity in terms of minimum detectable methane concentration is expressed as a function of birefringence, spectral transmission characteristics and polarization dependent loss of the fiber system, and the polarization and coherence characteristics of the light source. The sensitivity is calculated for various system arrangements and compared with existing experimental data. The model predicts that the interference effects may be minimized by using a depolarized broadband source (say, 30-50 nm) with a Gaussian spectral lineshape, together with a birefringence balanced sensor configuration.
A Dual-EDM Reverse Damascene Process for RF Switches and Other Bulk Metal Devices (2005)

This paper reports a manufacturing process suitable for lithography-compatible fabrication of suspended micro-structures from any conductive material (including bulk metal) that is available in sheet form. It uses two aligned steps of batch mode micro-electro-discharge machining followed by a series of filling and lapping steps similar to a damascene process. The process is demonstrated by the fabrication of a bi-stable RF switch from #302 stainless steel foil. The fabricated devices have a footprint of 5 × 5 mm2, with 25 μm thickness, and a minimum feature size of 5 μm.
Structure–activity relationships of methyl-lysine reader antagonists (2011)

The structure–activity relationships for small molecule antagonists of the Malignant Brain Tumor (MBT) domain family of methyl-lysine readers are described and activity demonstrated in histone peptide pull-down assays.
Corrections to Cationic Amphiphile with Shikimic Acid Headgroup Shows More Systemic Promise Than Its Mannosyl Analogue as DNA Vaccine Carrier in Dendritic Cell Based Genetic Immunization (2010)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionCorrections to Cationic Amphiphile with Shikimic Acid Headgroup Shows More Systemic Promise Than Its Mannosyl Analogue as DNA Vaccine Carrier in Dendritic Cell Based Genetic ImmunizationRamishetti Srinivas, Priya P. Karmali, Dipankar Pramanik, Arup Garu, Yenugonda Venkata Mahidhar, Bharat K. Majeti, Sistla Ramakrishna, Gunda Srinivas, and Arabinda Chaudhuri*Cite this: J. Med. Chem. 2010, 53, 7, 3003Publication Date (Web):March 5, 2010Publication History Published online5 March 2010Published inissue 8 April 2010https://pubs.acs.org/doi/10.1021/jm100267ehttps://doi.org/10.1021/jm100267ecorrectionACS PublicationsCopyright © 2010 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views783Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (550 KB) Get e-AlertscloseSUBJECTS:Amphiphiles,Cells,Genetics,Vaccination Get e-Alerts
Characterization and reactions of osmium(IV) ammines (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization and reactions of osmium(IV) amminesJohn D. Buhr, Jay R. Winkler, and Henry TaubeCite this: Inorg. Chem. 1980, 19, 8, 2416–2425Publication Date (Print):August 1, 1980Publication History Published online1 May 2002Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/ic50210a048https://doi.org/10.1021/ic50210a048research-articleACS PublicationsRequest reuse permissionsArticle Views283Altmetric-Citations46LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Measurement of the rate coefficient of the reaction CH+O2 ? products in the temperature range 2200 to 2600 K (1997)

The rate coefficient of the reaction CH+O2 → products was determined by measuring CH-radical concentration profiles in shock-heated 100–150 ppm ethane/1000 ppm O2 mixtures in Ar using cw, narrow-linewidth laser absorption at 431.131 nm. Comparing the measured CH concentration profiles to ones calculated using a detailed kinetics model, yielded the following average value for the rate coefficient independent of temperature over the range 2200–2600 K: The experimental conditions were chosen such that the calculated profiles were sensitive mainly to the reactions CH+O2 → products and CH3+M → CH+H2+M. For the methyl decomposition reaction channel, the following rate-coefficient expression provided the best fit of the measured CH profiles: Additionally, the rate coefficient of the reaction CH2+H→CH+ H2 was determined indirectly in the same system: © 1997 John Wiley & Sons, Inc.
<title>Technology development at the Center for Optics Manufacturing</title> (1992)

Center for Optics Manufacturing (COM) programs have matured beyond the framework of organizational activities. This paper describes COM's current activities and the industry, academic and government support that has made the program successful. The Center, established in 1989 at the University of Rochester's Institute of Optics, is a collaborative effort supported by American Precision Optics Manufacturers Association (APOMA), several academic institutions (Arizona, Central Florida, and Rochester) and the U.S. Army Material Command. The objective is to introduce innovative techniques and advance processing technology that will increase the competitiveness of the optics manufacturing base. Industry, academic, and government cooperation and support has been exceptional.
Identification of a peptide inhibitor for the histone methyltransferase WHSC1 (2018)

WHSC1 is a histone methyltransferase that is responsible for mono- and dimethylation of lysine 36 on histone H3 and has been implicated as a driver in a variety of hematological and solid tumors. Currently, there is a complete lack of validated chemical matter for this important drug discovery target. Herein we report on the first fully validated WHSC1 inhibitor, PTD2, a norleucine-containing peptide derived from the histone H4 sequence. This peptide exhibits micromolar affinity towards WHSC1 in biochemical and biophysical assays. Furthermore, a crystal structure was solved with the peptide in complex with SAM and the SET domain of WHSC1L1. This inhibitor is an important first step in creating potent, selective WHSC1 tool compounds for the purposes of understanding the complex biology in relation to human disease.
Tumor Antigens as Inappropriately Expressed Normal Alloantigens (1979)

Abstract Preliminary tests of three BALB/c myeloma tumors for ability to elicit immune responses in BALB/c mice showed that the only highly immunogenic one was MOPC-167. BALB/c mice immunized with this tumor developed cytotoxic T lymphocytes (CTLs) that lysed MOPC-167 cells specifically. When challenged with 5 × 105 MOPC-167 cells (about 1000 times the LD50 dose) labeled with 131I-iododeoxyuridine, tumors failed to develop in immunized animals and whole-body counts showed that the labeled MOPC-167 cells were rapidly eliminated. The cross-reactions of these CTL were unusual: they caused essentially no lysis of eight other BALB/c tumors, including three myeloma tumors, but they lysed extensively six out of nine tumors of DBA/2 mice (a seventh was lysed weakly). In contrast, the CTL elicited by MOPC-167 in (BALB/c × DBA/2)F1 hybrids did not lyse P815, the most sensitive of the DBA/2 tumors, and had less lytic activity against MOPC-167. CTL elicited by MOPC-167 in other BALB/c hybrids (BALB/c × NZB and BALB/c × C57BL/6) had the same reactions and cross-reactions as those elicited in purebred BALB/c mice. All of these observations suggest that MOPC-167 expresses an alloantigen that normally is absent in BALB/c mice but present in DBA/2 mice and in many DBA/2 tumors. The possibility that CTL elicited by immunization with a syngeneic tumor might be directed to inappropriately expressed alloantigens on the tumor cells was supported by observations with L5178Y: this lymphoma was one of the DBA/2 tumors not lysed by BALB/c anti-MOPC-167 CTL, but it was lysed by the CTL elicited in DBA/2 mice with MOPC-167 or with normal BALB/c spleen cells. Hence L5178Y, although of DBA/2 origin, appears to express antigens that are the same or strongly cross-reactive with an alloantigen that is absent in DBA/2 mice and present on normal BALB/c spleen cells and on a BALB/c myeloma tumor (MOPC-167).
Glycosylated Hemoglobin Levels in a Benign Form of Sickle Cell Anemia in Saudi Arabia (1986)

Glycosylated hemoglobin was determined by the thiobarbituric acid method in sickle cell anemia patients from the Eastern Province of Saudi Arabia. The level of glycosylated hemoglobin in a Saudi SS sample (4.36%, SD 0.83) is 90% of that of the sample of normals (4.85%, SD 0.51). This is in contrast with the reported value of glycosylated hemoglobin in an American Black SS sample (3.9%, SD 0.6), which is only 60% of that of the sample of normals (6.6%, SD 0.7). The fetal hemoglobin level in Saudi sickle cell patients was 12.03% (SD 4.84), which is significantly different from that of Americans of African origin at p = 0.001. There was no significant correlation (r = 0.236) between the percentages of glycosylated Hb and Hb F at the 10% confidence level. The reported positive relationship between the percentages of glycosylated Hb and Hb F in American Blacks seems to be valid in the Saudi population only up to the level of 10-12% of fetal hemoglobin. Above this threshold of Hb F no further alleviating effect is seen. The 2,3-diphosphoglycerate value in Saudi Hb SS adults was 21.7 mumol/g (SD 7.4) and accordingly only twice as high as that of normal individuals. The benign clinical course exhibited by Saudi sickle cell patients is reflected by the survival of the RBC as indexed by its content of glycosylated Hb and 2,3-diphosphoglycerate. Moreover 10-12% of fetal hemoglobin in the RBC seems sufficient to ameliorate the severity of this disease in patients from the Eastern Province of Saudi Arabia.
Quadrupedal locomotion: GasNets, CTRNNs and Hybrid CTRNN/PNNs compared (2004)

Evolutionary Robotics seeks to use evolutionary techniques to create both physical and physically simulated robots capable of exhibiting characteristics commonly associated with living organisms. Typically, biologically inspired artificial neural networks are evolved to act as sensorimotor control systems. These networks include; GasNets, Continuous Time Recurrent Neural Networks (CTRNNs) and Plastic Neural Networks (PNNs). This paper seeks to compare the performance of such networks in solving the problem of locomotion in a physically simulated quadruped. The results in this paper, taken together with those of other studies (summarized in this paper) help us to assess the relative strengths and weaknesses of the these three different approaches.
Selective Association of Peroxiredoxin 1 with Genomic DNA and COX-2 Upstream Promoter Elements in Estrogen Receptor Negative Breast Cancer Cells (2010)

In a search for proteins differentially cross-linked to DNA by cisplatin or formaldehyde in normal breast epithelial and breast cancer cell lines, we identified peroxiredoxin 1 (PRDX1) as a protein preferentially cross-linked to DNA in estrogen receptor negative (ER-) MDA-MB-231 but not in estrogen receptor positive (ER+) MCF7 breast cancer cells. Indirect immunofluorescence microscopic analyses showed that PRDX1 was located in the cytoplasm and nucleus of normal and breast cancer cells, with nuclear PRDX1 associated with promyelocytic leukemia protein bodies. We demonstrated that PRDX1 association with the transcription factor nuclear factor-kappaB (NF-kappaB) in MDA-MB-231 but not in MCF7 cells contributed to PRDX1-selective recruitment to MDA-MB-231 genomic DNA. Furthermore, PRDX1 was associated with the cyclooxygenase (COX)-2 upstream promoter region at sites occupied by NF-kappaB in ER- but not in ER+ breast cancer cells. PRDX1 knockdown attenuated COX-2 expression by reducing NF-kappaB occupancy at its upstream promoter element in MDA-MB-231 but not in MCF7 cells. A phosphorylated form of PRDX1 was only present in ER- breast cancer cells. Because PRDX1 phosphorylation is known to inhibit its peroxidase activity and to promote PRDX1 oligomerization, we propose that PRDX1 acts as a chaperone to enhance the transactivation potential of NF-kappaB in ER- breast cancer cells.
The Hepatoprotective and Therapeutic Effects of Propolis Ethanol Extract on Chronic Alcohol-induced Liver Injuries (1997)

Propolis designates a mixture of gums, resins and balms, of viscous consistency, which are gathered on certain parts (buds and bark, mainly) of vegetables (especially coniferous trees) by honeybees. They bring this back to the hive, where it is modified and mixed with other substances (essentially their own wax and salivary secretions). In this study, the hepatoprotective and therapeutic effects of propolis ethanol extract on chronic alcohol-induced liver injuries were investigated in rats. 3.125 ml of 99.5% alcohol was added to animal's daily diet for four weeks to induce chronic alcohol liver injuries. After sacrifice, serum transaminases (GOT, GPT), triacylglyceride and hepatic triacylglyceride (HTG) concentration were assayed to observe liver injuries induced by chronic alcohol abuse. In addition, the phenomenon of alcohol induced fatty liver were also observed by histopathological changes. Different doses of propolis ethanol extract were p.o. administered three times per day for three days, after four weeks' alcohol administration. It was found that 10 mg/kg of propolis ethanol extract significantly decreased the elevations of serum GOT, GPT, TG and HTG. In histopathological examination, 30 mg/kg of propolis ethanol extract also remarkably decreased the hepatocellular fatty degeneration, apparent as vacuolization, induced by chronic alcohol abuse.