PD4-09 VASOACTIVE INTESTINAL PEPTIDE AND PITUITARY ADENYLATE CYCLASE ACTIVATING PEPTIDE RECEPTOR 1 (VPAC1) TARGETED IMAGING OF PROSTATE CANCER: A PILOT STUDY (2015)

You have accessJournal of UrologyImaging/Radiology: Uroradiology I1 Apr 2015PD4-09 VASOACTIVE INTESTINAL PEPTIDE AND PITUITARY ADENYLATE CYCLASE ACTIVATING PEPTIDE RECEPTOR 1 (VPAC1) TARGETED IMAGING OF PROSTATE CANCER: A PILOT STUDY Edouard Trabulsi, Sushil Tripathi, Peter McCue, Ruth Birbe, Eric Wickstrom, Leonard Gomella, Sung Kim, Charles Intenzo, and Madhukar Thakur Edouard TrabulsiEdouard Trabulsi More articles by this author , Sushil TripathiSushil Tripathi More articles by this author , Peter McCuePeter McCue More articles by this author , Ruth BirbeRuth Birbe More articles by this author , Eric WickstromEric Wickstrom More articles by this author , Leonard GomellaLeonard Gomella More articles by this author , Sung KimSung Kim More articles by this author , Charles IntenzoCharles Intenzo More articles by this author , and Madhukar ThakurMadhukar Thakur More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.321AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Prostate cancer (CaP) overexpresses VPAC1, representing a highly suitable target for imaging and treatment. VPAC1 is over-expressed at the onset of the malignancy, which may be prior to elevation of PSA, and before cell morphology is altered. We have successfully used VPAC1 receptor-specific peptide constructs to image breast cancer in experimental animal models, and in humans. We hypothesized that VPAC1 expressed in high density on PC can be targeted for detection of intraprostatic tumor foci on whole mount radical prostatectomy specimens, using TP3805, a VPAC1 specific biomolecule labeled with Cu-64 a PET imaging radionuclide. METHODS As part of a PET imaging protocol targeting VPAC1, men (N=25) with prostate cancer undergoing radical prostatectomy were imaged preoperatively, and compared to whole mount radical prostatectomy pathologic analysis. De-paraffinized slides from whole mount pathology from two patients who participated the protocol, as well as one malignant lymph node and one benign lymph node specimens and 3 BPH negative controls underwent digital autoradiography with Cu-64, and then were H&E stained. Autoradiography images were compared with prostate H&E staining in which tumor foci were delineated. RESULTS Prostate cancer foci (n=30/31) were identified by autoradiography imaging (Figure 1). Autoradiography missed one malignant lesion due to technical artifact. Additionally, 6 small cancerous lesions were identified by autoradiography that were not previously noted by histologic examinations. A total of 7 additional lesions seen by autoradiography corresponded with HGPIN. The positive lymph node and the benign lymph node were correctly identified by auto radiography (Fig.2). For the 3 BPH patients, no cancer foci were noted by autoradiography. CONCLUSIONS VPAC1 peptide analog constructs accurately identified foci of prostate cancer on whole mount radical prostatectomy specimens. Several additional lesions were also identified. Detection of HGPIN is consistent with the early expression of VPAC1 prior to the modulations in cell morphology. The PPV (97%) and NPV(100%) were excellent, validating VPAC1 as a potential theragnostic target for prostate cancer imaging and treatment. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e90 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.Metrics Author Information Edouard Trabulsi More articles by this author Sushil Tripathi More articles by this author Peter McCue More articles by this author Ruth Birbe More articles by this author Eric Wickstrom More articles by this author Leonard Gomella More articles by this author Sung Kim More articles by this author Charles Intenzo More articles by this author Madhukar Thakur More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...