gebhart/patrepeval huggingface dataset:
Enhancer remodeling drives MLL oncogene-dependent transcriptional dysregulation in leukemia stem cells (2023)

Acute myeloid leukemia (AML) with mixed-lineage leukemia (MLL) gene rearrangement (MLLr) comprises a cellular hierarchy in which a subpopulation of cells serves as functional leukemia stem cells (LSCs). They are maintained by a unique gene expression program and chromatin states, which are thought to reflect the actions of enhancers. Here, we delineate the active enhancer landscape and observe pervasive enhancer malfunction in LSCs. Reconstruction of regulatory networks revealed a master set of hematopoietic transcription factors. We show that EP300 is an essential transcriptional coregulator for maintaining LSC oncogenic potential because it controls essential gene expression through modulation of H3K27 acetylation and assessments of transcription factor dependencies. Moreover, the EP300 inhibitor A-485 affects LSC growth by targeting enhancer activity via histone acetyltransferase domain inhibition. Together, these data implicate a perturbed MLLr-specific enhancer accessibility landscape, suggesting the possibility for disruption of the LSC enhancer regulatory axis as a promising therapeutic strategy in AML.
Glucosamine-Induced Endoplasmic Reticulum Dysfunction Is Associated With Accelerated Atherosclerosis in a Hyperglycemic Mouse Model (2006)

Diabetes is a major independent risk factor for cardiovascular disease and stroke; however, the molecular and cellular mechanisms by which diabetes contributes to the development of vascular disease are not fully understood. Our previous studies demonstrated that endoplasmic reticulum (ER) stress-inducing agents, including homocysteine, promote lipid accumulation and activate inflammatory pathways-the hallmark features of atherosclerosis. We hypothesize that the accumulation of intracellular glucosamine observed in diabetes may also promote atherogenesis via a mechanism that involves ER stress. In support of this theory, we demonstrate that glucosamine can induce ER stress in cell types relevant to the development of atherosclerosis, including human aortic smooth muscle cells, monocytes, and hepatocytes. Furthermore, we show that glucosamine-induced ER stress dysregulates lipid metabolism, leading to the accumulation of cholesterol in cultured cells. To examine the relevance of the ER stress pathway in vivo, we used a streptozotocin-induced hyperglycemic apolipoprotein E-deficient mouse model of atherosclerosis. Using molecular biological and histological techniques, we show that hyperglycemia is associated with tissue-specific ER stress, hepatic steatosis, and accelerated atherosclerosis. This novel mechanism may not only explain how diabetes and hyperglycemia promote atherosclerosis, but also provide a potential new target for therapeutic intervention.
Idiopathic Prepubertal Short Stature Is Associated with Low Body Mass Index (1993)

The hypothesis that insufficient body weight is partly responsible for idiopathic short stature was tested by evaluating 79 prepubertal children having idiopathic short stature, classified according to their body mass index (BMI) zs: group 1 BMI zs < or = 0 (m +/- SE, -0.9 +/- 0.1, n = 53) and group 2 BMI zs > 0 (0.6 +/- 0.1, n = 26). Their ages were similar (8.1 +/- 0.3 and 8.4 +/- 0.4 years). The following parameters were significantly lower in group 1 than in group 2: height (-2.5 +/- 0.1 vs. -2.2 +/- 0.1 SD, p < 0.05), height velocity (-1.5 +/- 0.2 vs. -0.9 +/- 0.4 SD, p < 0.05), bone age (bone age retardation 2.3 +/- 0.2 vs. 1.3 +/- 0.4 years, p < 0.005) and plasma insulin-like growth factor 1 (IGF-1, 0.8 +/- 0.1 vs. 1.2 +/- 0.1 U/ml, p < 0.05). The BMI was significantly lower in children with idiopathic short stature (-0.4 +/- 0.1) than in the normal population (zs, p < 0.0002). BMI zs was positively correlated with height (p < 0.005), height velocity (p < 0.05) and plasma IGF-1 (p < 0.01). We conclude that children with idiopathic short stature are leaner than the normal population. An inadequate or insufficient nutritional intake may be partly responsible for idiopathic short stature. The effects of improved intake on the height change are under evaluation.
Adaptive Morphology for Multi-Modal Locomotion (2015)

There is a growing interest in using robots in dangerous environments, such as for exploration, search-and-rescue or monitoring applications, in order to reduce the risks for workers or rescuers and to improve their efficiency. Typically, flying robots offer the possibility to quickly explore large areas while ground robots can thoroughly search specific regions of interest. While existing robotic solutions are very promising, they are often limited to specific use cases or environments. This makes them impractical for most missions involving complex or unpredictable scenarios, such as search-and-rescue applications. This limitation comes from the fact that existing robots usually exploit only a single locomotion strategy, which limits their flexibility and adaptability to different environments. In this thesis, a multi-modal locomotion strategy is investigated as a way to increase the versatility of mobile robots. We explore integrated design approaches, where the same actuators and structure are used for different modes of locomotion, which allows a minimization of the weight and complexity of the robot. This strategy is challenging because a single locomotor system must accommodate the potentially conflicting dynamics of multiple modes of locomotion. Herein, we suggest taking inspiration from nature, in particular the common vampire bat \emph{Desmodus rotundus}. The goal being to make multiple modes of locomotion dynamically compatible (i.e. have compatible speeds and torques requirements), by optimizing the morphology of the locomotor system and even by adapting the morphology of the robot to a specific mode of locomotion. It is demonstrated in this thesis that the integrated design approach can be effectively implemented on a multi-modal aerial and terrestrial robot, and that two modes of locomotion can be made dynamically compatible by optimizing the morphology. Furthermore, an adaptive morphology is used to increase the efficiency of the different modes of locomotion. A locomotor system used both for walking on the ground and controlling flight, has been successfully implemented on a multi-modal robot, which further has deployable wings to increase its performances on the ground and in the air. By successfully exploiting the concepts of integrated design and adaptive morphology, this robot is capable of hovering, forward flight and ground locomotion. This robot demonstrates a very high versatility compared to state of the art of mobile robots, while having a low complexity.
Effects of amino acid substitutions at the active site in Escherichia coli beta-galactosidase. (1988)

Forty-nine amino acid substitutions were made at four positions in the Escherichia coli enzyme beta-galactosidase; three of the four targeted amino acids are thought to be part of the active site. Many of the substitutions were made by converting the appropriate codon in lacZ to an amber codon, and using one of 12 suppressor strains to introduce the replacement amino acid. Glu-461 and Tyr-503 were replaced, independently, with 13 amino acids. All 26 of the strains containing mutant enzymes are Lac-. Enzyme activity is reduced to less than 10% of wild type by substitutions at Glu-461 and to less than 1% of wild type by substitutions at Tyr-503. Many of the mutant enzymes have less than 0.1% wild-type activity. His-464 and Met-3 were replaced with 11 and 12 amino acids, respectively. Strains containing any one of these mutant proteins are Lac+. The results support previous evidence that Glu-461 and Tyr-503 are essential for catalysis, and suggest that His-464 is not part of the active site. Site-directed mutagenesis was facilitated by construction of an f1 bacteriophage containing the complete lacZ gene on a single EcoRI fragment.
The Role of Rehabilitation in Arterial Function Properties of Convalescent COVID-19 Patients (2023)

Coronavirus disease (COVID-19) is a respiratory disease, although arterial function involvement has been documented. We assess the impact of a post-acute COVID-19 rehabilitation program on endothelium-dependent vasodilation and arterial wall properties. We enrolled 60 convalescent patients from COVID-19 and one-month post-acute disease, who were randomized at a 1:1 ratio in a 3-month cardiopulmonary rehabilitation program (study group) or not (control group). Endothelium-dependent vasodilation was evaluated by flow-mediated dilation (FMD), and arterial wall properties were evaluated by carotid-femoral pulse wave velocity (cf-PWV) and augmentation index (AIx) at 1 month and at 4 months post-acute disease. FMD was significantly improved in both the study (6.2 ± 1.8% vs. 8.6 ± 2.4%, <i>p</i> < 0.001) and control groups (5.9 ± 2.2% vs. 6.6 ± 1.8%, <i>p</i> = 0.009), but the improvement was significantly higher in the study group (rehabilitation) (<i>p</i> < 0.001). PWV was improved in the study group (8.2 ± 1.3 m/s vs. 6.6 ± 1.0 m/s, <i>p</i> < 0.001) but not in the control group (8.9 ± 1.8 m/s vs. 8.8 ± 1.9 m/s, <i>p</i> = 0.74). Similarly, AIx was improved in the study group (25.9 ± 9.8% vs. 21.1 ± 9.3%, <i>p</i> < 0.001) but not in the control group (27.6 ± 9.2% vs. 26.2 ± 9.8 m/s, <i>p</i> = 0.15). Convalescent COVID-19 subjects of the study group (rehabilitation) with increased serum levels of circulating IL-6 had a greater reduction in FMD. Conclusively, a 3-month cardiopulmonary post-acute COVID-19 rehabilitation program improves recovery of endothelium-dependent vasodilation and arteriosclerosis.
Supplementary Figures 1-8 from <i>SETD2</i> Is Recurrently Mutated in Whole-Exome Sequenced Canine Osteosarcoma (2023)

&lt;p&gt;Supplementary Figure 1 - Workflow for Somatic Point and Indel Mutation detection. Supplementary Figure 2 - Heatmap counts of samples with recurrent focal SCNAs per chromosome. Supplementary Figure 3 - oaCGH profiles penetrance plots and GISTIC analysis. Supplementary Figure 4 - Venn diagrams of shared and unique somatic point mutations and somatic indel mutations across the three breeds. Supplementary Figure 5 - Scatter plot shows age of diagnosis is positively correlated to the mutation counts across all breeds. Supplementary Figure 6 - Mutational signature distribution per breed shows enrichment for the novel signature in Golden Retriever. Supplementary Figure 7 - Overview and functional classes for the mutations in histone modifier genes across the three breeds. Supplementary Figure 8 - Overview of germ-line candidate mutation across three breeds and their functional impacts.&lt;/p&gt;
A Teradata content-based multimedia object manager for massively parallel architectures (1996)

The Teradata Multimedia Object Manager is a general-purpose content analysis multimedia server designed for symmetric multiprocessing and massively parallel processing environments. The Multimedia Object Manager defines and manipulates user-defined functions (UDFs), which are invoked in parallel to analyze or manipulate the contents of multimedia objects. Several computationally intensive applications of this technology, which use large persistent datasets, include fingerprint matching, signature verification, face recognition, and speech recognition/translation.
Hydrogenolysis of Lignin (1966)

The structural features of Alnus rubra lignin, isolated by mild hydrogenolysis over Raney nickel, have been examined primarily by nuclear magnetic resonance (NMR) spectroscopy techniques. The total lignin isolated using acidic catalysis is estimated to contain 34% syringyl units, 23% condensed guaiacyl units, and 43% guaiacyl units. A high molecular weight fraction (of this hydrol lignin) is estimated to contain 15% syringyl units, 51% condensed guaiacyl units, and 34% guaiacyl units. Identified monomeric products contain more than 99% C6C3 structures. The lignin isolated using basic catalysis apparently underwent some aromatic ring reduction and extensive side-chain degradation. NMR and gas chromatography techniques are discussed.
Optical Interconnects and Extreme Computing (2016)

order-creating-national-strategic-computing-initiative Some believe that interconnects inevitably will need to employ WDM and integrated photonics, because of the scale promised by these technologies.
Mechanistic Study of 17α-Ethinylestradiol Biodegradation by Pleurotus ostreatus: Tracking of Extracelullar and Intracelullar Degradation Mechanisms (2012)

The white rot fungus Pleurotus ostreatus is able to completely remove the synthetic hormone 17α-ethinylestradiol (EE2, 200 μg in 20 mL) from a liquid complex or mineral medium in 3 or 14 days, respectively. Its efficiency has also been documented in the removal of estrogenic activity that correlated with the EE2 degradation. A set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that EE2 was degraded by isolated laccase (about 90% within 24 h). The degradation was also tested with concentrated extracellular liquid where degradation reached 50% mainly due to the laccase activity; however, after a supplementation with H₂O₂ and Mn²⁺, residual manganese-dependent peroxidase activities (40 times lower than Lac) raised the degradation to 100%. Moreover, the intracellular fraction and also laccase-like activity associated with fungal mycelium were found to be efficient in the degradation too. Isolated microsomal proteins appeared to also be involved in the process. The degradation was completely suppressed in the presence of cytochrome P-450 inhibitors, piperonylbutoxide and carbon monoxide, indicating a role of this monooxygenase in the degradation process. Attention was also paid to monitoring of changes in the estrogenic activity during these particular in vitro experiments when mainly degradations related to ligninolytic enzymes were found to decrease the estrogenic activity with EE2 removal proportionally. Several novel metabolites of EE2 were detected using different chromatographic method with mass spectrometric techniques (LC-MS, GC-MS) including also [¹³C]-labeled substrates. The results document the involvement of various different simultaneous mechanisms in the EE2 degradation by P. ostreatus by both the ligninolytic system and the eukaryotic machinery of cytochromes P-450.
Frequency domain analysis of triggered lightning return stroke luminosity velocity (2017)

Abstract Fourier analysis is applied to time domain return stroke luminosity signals to calculate the phase and group velocities and the amplitude of the luminosity signals as a function of frequency measured between 4 m and 115 m during 12 triggered lightning strokes. We show that pairs of time domain luminosity signals measured at different heights can be interpreted as the input and the output of a system whose frequency domain transfer function can be determined from the measured time domain signals. From the frequency domain transfer function phase we find the phase and group velocities, and luminosity amplitude as a function of triggered lightning channel height and signal frequency ranging from 50 kHz to 300 kHz. We show that higher‐frequency luminosity components propagate faster than the lower frequency components and that higher‐frequency luminosity components attenuate more rapidly than lower frequency components. Finally, we calculate time domain return stroke velocities as a function of channel height using two time delay techniques: (1) measurement at the 20% amplitude level and (2) cross correlation.
Cataracts and Allergic Encephalomyelitis (1981)

Juvenile guinea pigs developed cataracts during severe acute allergic (autoimmune) encephalomyelitis produced actively by injection of central nervous system antigens in complete Freund’s adjuvants. Cataracts were induced in immature guinea pigs by passive transfer of living lymph node cells from sensitized strain 13 donors to histocompatible recipients. Cataracts were not induced in adult guinea pigs either passively or actively.
ChemInform Abstract: Efficient and Practical Asymmetric Synthesis of the Taxol C‐13 Side Chain, N‐Benzoyl‐(2R,3S)‐3‐phenylisoserine, and Its Analogues via Chiral 3‐Hydroxy‐4‐aryl‐β‐lactams Through Chiral Ester Enolate. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Carotid Ultrasound in Assessing Fluid Responsiveness in Patients with Hypotension and Suspected Sepsis (2021)

In patients with suspected sepsis, a fluid challenge resulted in a significant change in CFTc, but not CBF. Neither absolute measurement nor delta measurements with fluid challenge predicted clinical outcomes.
ChemInform Abstract: Marine Pyridoacridine Alkaloids: Biosynthesis and Biological Activities (2016)

Abstract Review: 58 refs.
Postpartum home blood pressure monitoring and lifestyle intervention in overweight and obese individuals the first year after gestational hypertension or pre‐eclampsia: A pilot feasibility trial (2023)

In this pilot, randomised trial, we demonstrate feasibility of HBPM paired with a lifestyle intervention in the first year postpartum. We detected high rates of ongoing hypertension, emphasising the need for the development of effective interventions in this population.
Spatially strain‐induced and selective preparation of Mo x N ( x  = 1, 2) as a highly effective nanoarchitectonic catalyst for hydrogen evolution reaction in a wide pH range (2023)

Developing highly efficient catalysts for the hydrogen evolution reaction (HER) is crucial to commercial water splitting in the global efforts to mitigate fossil fuel combustion and combat global climate change. Molybdenum nitrides (Mo x N) such as γ‐Mo 2 N and δ‐MoN are promising HER catalysts. Although δ‐MoN has better HER characteristics, controllable preparation of the materials is still challenging. Herein, selective preparation of γ‐Mo 2 N and δ‐MoN is demonstrated by controlling the spatial stress. The hybrid δ‐MoN and N‐doped carbon composite (MoN/NC) consists of MoN layers and 1‐nm‐thick carbon layers. The carbon layers polarized by the high valence state of Mo in MoN provide the adsorption sites for H + , and the NC layers also facilitate electron transport during the catalytic process. As a result, MoN/NC exhibits remarkable HER activity such as low overpotentials of 93, 211 and 141 mV to attain a current density of 10 mA·cm −2 as well as small Tafel slopes of 44.5, 83.2 and 65.4 mV·dec −1 in acidic, neutral and basic electrolytes of 0.5 mol·L −1 H 2 SO 4 , 1 mol·L −1 PBS, and 1 mol·L −1 KOH, respectively. The spatial stress effects enable selective preparation of specific phases in catalysts, and the pertinent mechanism provides important guidance to the preparation and optimization of advanced catalysts.
TMIC-66. FIBROBLAST ACTIVATION PROTEIN: TISSUE EXPRESSION, POSITRON-EMISSION TOMOGRAPHY UPTAKE AND ITS PROGNOSTIC SIGNIFICANCE IN GLIOSARCOMAS AND GLIOBLASTOMAS (2022)

Abstract BACKGROUND Although histologically distinct, gliosarcomas and glioblastomas are treated according to the same standards. Fibroblast-activation-protein (FAP) is a component of a tumor-specific subpopulation of fibroblasts that play a critical role in tumor growth and invasion. Few case studies suggest an elevated expression of FAP in glioblastoma and a particularly strong expression in gliosarcoma, possibly for its predominant mesenchymal differentiation. However, the prognostic impact of FAP in glioblastoma and gliosarcoma is unclear. Question:What is the clinical relevance of FAP-expression in gliosarcoma and glioblastoma and how does it correlate with FAPI46-PET-uptake? METHODS In a retrospective analysis, patients diagnosed with glioblastoma and gliosarcoma were enrolled. Histological examination was performed using immunohistochemical FAP-staining and quantitative analysis with standardized FAP-Score (0 to 3,with higher values indicating stronger expression). In a subset of patients, FAPI46-PETs have been performed. The SUV values are planned be correlated with FAP-expression in the tumor tissue. Data obtained from immunohistochemical analysis and SUV values are planned to be brought into perspective with clinically relevant prognostic factors, including survival estimates. RESULTS A total of 45 patients have been enrolled. On immunohistochemical staining, gliosarcomas expressed significantly more FAP (average FAP-Score,2.3) than glioblastomas (average FAP-Score,0.7,p&amp;lt; 0.0001). While in glioblastoma FAP-expression was confined to the perivascular space, in gliosarcoma the neoplastic cells themselves -in addition to the perivascular extracellular matrix- expressed FAP. Initial analyses indicate that tissue FAP-expression is correlated with tracer uptake on FAPI46-PET. Further studies on clinical relevance using correlation to clinical data are currently underway. CONCLUSIONS This study indicates that FAP-expression is much more abundant in gliosarcomas as opposed to glioblastomas. This could open not only a diagnostic but also a therapeutic gap. The prognostic role and the association of FAP-tissue expression with FAPI46-PET tracer uptake will be reported at the time of abstract presentation.
An advanced 3D electronic packaging design for the CONTOUR remote imaging spectrograph digital processor unit (2002)

The data processing unit (DPU) of the CONTOUR remote imaging spectrograph (CRISP) is being developed by the Johns Hopkins University Applied Physics Laboratory for the Comet Nucleus Tour (CONTOUR), a NASA Discovery mission to explore the composition of the comets Encke and Schwassmann-Wachmann 3. The DPU design employs an advanced electronic packaging approach with 3-D interconnect that provides flexibility to accommodate future expansion and modification by simply adding or replacing one of its modules. Miniaturization of space electronics involves considerations beyond the usual requirements for small size and low mass. A system approach must be developed and started with the top down deliberations on the design of an electronic architecture suitable for miniaturizing all physical aspects of the electronics design, and continued with considerations at the board, component and chips levels. One important task that has often been ignored, in an electronic package design, is the selection of the connectors and the interconnect architecture between the individual boards. This paper will describe the DPU packaging design, the selection process of the interconnect architecture, and the reliability evaluation of the press-fit connectors used in this design.
Dr. Joseph C. Bequaert (1965)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.