gebhart/patrepeval huggingface dataset:
Cloning and Characterization of a Novel Human Phosphodiesterase That Hydrolyzes Both cAMP and cGMP (PDE10A) (1999)

cDNA encoding a novel phosphodiesterase (PDE) was isolated from a human fetal lung cDNA library and designated PDE10A. The deduced amino acid sequence contains 779 amino acids, including a putative cGMP binding sequence in the amino-terminal portion of the molecule and a catalytic domain that is 16-47% identical in amino acid sequence to those of other PDE families. Recombinant PDE10A transfected and expressed in COS-7 cells hydrolyzed cAMP and cGMP with Km values of 0.26 and 7.2 microM, respectively, and Vmax with cGMP was almost twice that with cAMP. Of the PDE inhibitors tested, dipyridamole was most effective, with IC50 values of 1.2 and 0.45 microM for inhibition of cAMP and cGMP hydrolysis, respectively. cGMP inhibited hydrolysis of cAMP, and cAMP inhibited cGMP hydrolysis with IC50 values of 14 and 0.39 microM, respectively. Thus, PDE10A exhibited properties of a cAMP PDE and a cAMP-inhibited cGMP PDE. PDE10A transcripts were particularly abundant in the putamen and caudate nucleus regions of brain and in thyroid and testis, and in much lower amounts in other tissues. The PDE10A gene was located on chromosome 6q26 by fluorescent in situ hybridization analysis. PDE10A represents a new member of the PDE superfamily, exhibiting unique kinetic properties and inhibitor sensitivity.
Identification of botanicals with potential therapeutic use against methicillin‐resistant Staphylococcus aureus (MRSA) infections (2018)

Staphylococcus aureus is an opportunistic pathogen. Over- and improper-use of pharmaceuticals against S. aureus has led to the development of antibiotic resistance, including methicillin-resistant S. aureus (MRSA). This study examined the efficacy of botanical extracts as an alternative form of treatment to S. aureus and MRSA, including penicillin/methicillin-resistant S. aureus (Pen<sup>R</sup> ), and multidrug resistant S. aureus (MDR). Initial screening of botanicals was done via a minimum inhibitory concentration procedure. In addition, a temporal growth curve was performed in order to quantify the growth of the bacteria in the presence of the extracts. Results demonstrated 13 botanicals that had varying activities against S. aureus, Pen<sup>R</sup> , and MDR. These botanicals were separated into mild, moderate, and highly efficacious based on the concentration needed to inhibit bacterial growth. These results propose a comparison of botanical-derived antimicrobial extracts that may be utilized against S. aureus and different antibiotic resistant strains of MRSA.
Electronic Structure and Chemical Reactivity of Carbon Nanotubes: A Chemist's View (2004)

A qualitative description of the electronic structure of single-wall carbon nanotubes from a chemical perspective is presented using real-space orbital representations and traditional concepts of aromaticity, orbital symmetry and frontier orbitals. This unusual view of carbon nanotubes allows us to merge the solid-state physics description of band structures with the molecular orbitals framework of reaction mechanisms used in organic chemistry and to predict intriguing chemical selectivity based on electronic structure.
Regulation of renal CYP4A expression and 20-HETE synthesis by nitric oxide in pregnant rats (2003)

20-Hydroxyeicosatetraenoic acid (20-HETE), which promotes renal vasoconstriction, is formed in the rat kidney primarily by cytochrome P-450 (CYP) 4A isoforms (4A1, 4A2, 4A3, 4A8). Nitric oxide (NO) has been shown to bind to the heme moiety of the CYP4A2 protein and to inhibit 20-HETE synthesis in renal arterioles of male rats. However, it is not known whether NO interacts with and affects the activity of CYP4A1 and CYP4A3, the major renal CYP4A isoforms in female rats. Incubation of recombinant CYP4A1 and 4A3 proteins with sodium nitroprusside (SNP) shifted the absorbance at 440 nm, indicating the formation of a ferric-nitrosyl-CYP4A complex. The absorbance for CYP4A3 was about twofold higher than that of CYP4A1. Incubation of SNP or peroxynitrite (PN; 0.01-1 mM) with CYP4A recombinant membranes caused a concentration-dependent inhibition of 20-HETE synthesis, with both chemicals having a greater inhibitory effect on CYP4A3-catalyzed activity. Moreover, incubation of CYP4A1 and 4A3 proteins with PN (1 mM) resulted in nitration of tyrosine residues in both proteins. In addition, PN and SNP inhibited 20-HETE synthesis in renal microvessels from female rats by 65 and 59%, respectively. We previously showed that microvessel CYP4A1/CYP4A3 expression and 20-HETE synthesis are decreased in late pregnancy. Therefore, we investigated whether such a decrease is dependent on NO, the synthesis of which has been shown to increase in late pregnancy. Administration of NG-nitro-l-arginine methyl ester (l-NAME) to pregnant rats for 6 days (days 15-20 of pregnancy) caused a significant increase in systolic blood pressure, which was prevented by concurrent treatment with the CYP4A inhibitor 1-aminobenzotriazole (ABT). Urinary NO2/NO3 excretion decreased by 40 and 52% in l-NAME- and l-NAME + ABT-treated groups, respectively. Interestingly, renal microvessel 20-HETE synthesis showed a marked increase following l-NAME treatment, and this increase was diminished with coadministration of ABT. These results demonstrate that NO interacts with CYP4A proteins in a distinct manner and it interferes with renal microvessel 20-HETE synthesis, which may play an important role in the regulation of blood pressure and renal function during pregnancy.
Prognostic significance of ventricular CSF lactic acidosis in severe head injury (1986)

✓ Brain-tissue acidosis inferred by cerebrospinal fluid (CSF) lactic acidosis is considered to play an important role in the clinical course of severe head injury. Ventricular CSF lactate concentration was studied in 19 patients during the first 5 days after severe head injury. All patients were intubated, paralyzed, and artificially ventilated so that PaCO 2 was kept at 33.2 ± 5.0 mm Hg and PaO 2 at 122 ± 18 mm Hg (mean ± standard deviation). The mean Glasgow Coma Scale score on admission was 5.73 ± 2.42. The first CSF sample was drawn within 18 hours after head injury. Over the first 4 days postinjury, patients with a poor outcome had significantly higher ventricular CSF lactate levels than did those with moderate disabilities or a good outcome. Patients showing favorable outcome had a significant decrease in ventricular CSF lactate levels 48 hours after injury. This decrease was not observed in patients with a poor outcome. Increased ventricular CSF lactate concentration was also reliably associated with increased intracranial pressure (ICP). Ventricular CSF lactate levels did not correlate with the magnitude of intraventricular bleeding. Arterial and jugular venous blood lactate levels, although high after head injury, were usually lower than the levels in the ventricular CSF and reached a normal range by the 3rd day following head trauma. At that time, the ventricular CSF lactate concentration was still above normal in patients with a poor outcome but had decreased to normal in patients with moderate disabilities or a good outcome. Ventricular CSF pH did not generally correlate with the ventricular CSF lactate concentration in patients under controlled ventilation; however, in a few patients close to death or with ventricular infection, a correlation was noted. Ventricular CSF lactate levels were not related to cerebral blood flow. In this study, profiles of ventricular CSF lactate concentration are defined in relation to the patients' clinical course and outcome. High ventricular CSF lactate concentration is present within 18 hours after severe head injury. Its decrease to normal in the following 48 hours is a reliable sign of clinical improvement; however, ventricular CSF lactate levels that are persistently high or that increase over time indicate the patient's deterioration. Serial assessment of ventricular CSF for acid-base status and metabolites in head-injured patients with a ventricular catheter already placed for ICP monitoring is useful in the evaluation of prognosis and clinical course.
Mutable elastic models for sculpting structured shapes (2013)

Abstract In this paper, we propose a new paradigm for free‐form shape deformation. Standard deformable models minimize an energy measuring the distance to a single target shape. We propose a new, “mutable” elastic model. It represents complex geometry by a collection of parts and measures the distance of each part measures to a larger set of alternative rest configurations. By detecting and reacting to local switches between best‐matching rest states, we build a 3D sculpting system: It takes a structured shape consisting of parts and replacement rules as input. The shape can subsequently be elongated, compressed, bent, cut, and merged within a constraints‐based free‐form editing interface, where alternative rest‐states model to such changes. In practical experiments, we show that the approach yields a surprisingly intuitive and easy to implement interface for interactively designing objects described by such discrete shape grammars, for which direct shape control mechanisms were typically lacking.
Atomic waste molten into glass (1975)

It is reported on highly radioactive wastes, on the solidification, the technology of the solidification of highly radioactive waste solutions as well as on an outlook. A table of the chemical composition of the highly radioactive waste solution (KEWA standard, 3,600 Ci/ltr.) is presented. (HK)
Bilateral Breast Reconstruction (1994)

Baldwin, Bonnie J. M.D.; Schusterman, Mark A. M.D.; Miller, Michael J. M.D.; Kroll, Stephen S. M.D.; Wang, Bao-guang M.D., M.P.H.; Hoffman, Saul M.D.Author Information
Transcription Vectors That Facilitate the Identification and Mapping of RNA Splice Sites in Genomic DNA (1990)

Two transcription vectors were constructed that can identify the splice sites at exon-intron boundaries of inserted DNA fragments possessing the complementary splice site. One vector contains the 5' splice donor site and flanking exon-intron sequences from the 3' end of the adenovirus first late leader. The other vector contains the 3' splice acceptor site and the branch acceptor site, plus the flanking exon-intron sequences from the 5' end of the adenovirus second late leader. Both vectors contain a multiple cloning site for insertion of DNA fragments. DNA fragments supplying the complementary splice site, including the adjacent exon and intron sequences, were inserted into the vectors. The vectors were used as templates for the synthesis of chimeric RNA transcripts that were spliced in in vitro splicing extracts. Chimeric transcripts from the vectors containing complementary splice site boundary regions from the human growth hormone gene were accurately spliced in vitro. A splice site from a human growth hormone intron that is not normally spliced in vitro was spliced when paired with an adenovirus splice site. These vectors can be used to identify splice sites and to determine the lengths of exons and their attached introns within a DNA fragment of unknown coding content.
Paraneoplastic Cerebellar Degeneration and Lambert-Eaton Myasthenic Syndrome Associated with Neuroendocrine Carcinoma of the Oropharynx (2017)

Paraneoplastic cerebellar degeneration and Lambert-Eaton myasthenic syndrome (PCD-LEMS) are usually associated with small-cell lung carcinoma (SCLC). PCD-LEMS with extrapulmonary non-SCLC tumors; however, has not been previously reported. A 78-year-old man presented with dysarthria, dysphagia, staggering gait, and lower extremity muscle fatigue. He was diagnosed with PCD-LEMS associated with neuroendocrine carcinoma of the oropharynx, based on the histological findings of the biopsy, the existence of antibodies against P/Q-type voltage-gated calcium channels, and an incremental response of the compound muscle action potentials during repetitive nerve stimulation tests. Thus, PCD-LEMS should be included in the differential diagnosis of neurological dysfunction, even in extrapulmonary non-SCLC patients.
Quantification of Parasite Load in Clinical Samples of Leishmaniasis Patients: IL-10 Level Correlates with Parasite Load in Visceral Leishmaniasis (2010)

A rapid and accurate method to detect and quantify Leishmania parasite is urgently needed to facilitate early diagnosis of leishmaniasis and monitoring of antileishmania therapy. In this study, real-time assay was applied to estimate parasite load in clinical samples of visceral leishmaniasis (VL) and post kala-azar dermal leishmaniasis (PKDL) patients. The mean parasite load in blood of VL patients (n = 31) was 8,372 parasites/ml, while the mean parasite load in bone marrow aspirate (BMA) was 194,962 parasites/million nucleated cells (n = 12). Parasite load was undetectable after treatment with amphotericin B (n = 9) in VL, while a residual parasite burden was detected in 2 of 6 patients following treatment with sodium antimony gluconate. Further, circulating levels of IFN-gamma, TNF-alpha, IL-10, IL-6, IL-4 and IL-2 were analysed in VL patients (n = 29) by Cytometric Bead Array to evaluate correlation with parasitic load. Interestingly, IL-10 levels correlated significantly with parasite load (r = 0.82, P<0.0001). The mean parasite load in dermal lesions of PKDL patients was 9,502 parasites/microg tissue DNA at pre-treatment stage (n = 25), with no detectable parasites after therapy (n = 5). Parasite burden was distinctly higher (P<0.0001) in nodular lesions (n = 12) (19,586 parasites/microg tissue DNA) compared to papular/macular lesions (n = 13, 193 parasites/microg tissue DNA). Further, chronic PKDL lesions showed significantly (P = 0.0166) higher parasite load in comparison with acute lesions. Results indicate that chronic, nodular cases constitute the major parasite reservoir for anthroponotic transmission. Our results establish that the high parasite load in VL is strongly correlated with a high level of IL-10, implicating IL-10 as a marker of disease severity. The assay is applicable for diagnosis as well as prognosis of both VL and PKDL, providing a simple molecular tool to monitor the efficacy of antileishmanial drugs or vaccines.
The p.Arg435His Variation of IgG3 With High Affinity to FcRn Is Associated With Susceptibility for Pemphigus Vulgaris—Analysis of Four Different Ethnic Cohorts (2018)

IgG3 is the IgG subclass with the strongest effector functions among all four IgG subclasses and the highest degree of allelic variability among all constant immunoglobulin genes. Due to its genetic position, IgG3 is often the first isotype an antibody switches to before IgG1 or IgG4. Compared with the other IgG subclasses, it has a reduced half-life which is probably connected to a decreased affinity to the neonatal Fc receptor (FcRn). However, a few allelic variants harbor an amino acid replacement of His435 to Arg that reverts the half-life of the resulting IgG3 to the same level as the other IgG subclasses. Because of its functional impact, we hypothesized that the p.Arg435His variation could be associated with susceptibility to autoantibody-mediated diseases like pemphigus vulgaris (PV) and bullous pemphigoid (BP). Using a set of samples from German, Turkish, Egyptian, and Iranian patients and controls, we were able to demonstrate a genetic association of the p.Arg435His variation with PV risk, but not with BP risk. Our results suggest a hitherto unknown role for the function of IgG3 in the pathogenesis of PV.
Het platteland telt (weer) mee (1998)

Item does not contain fulltext
ChemInform Abstract: Synthesis and Thermal Reactions of Perfluoroalkylsulfonyl‐Substituted Ylides. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Psychological analysis on human-robot interaction (2002)

For realizing a robot working in human society, interaction with humans is the key issue. We have developed a robot that interacts with humans based on visual recognition. This robot has two vision systems: an omnidirectional vision system for acquiring necessary visual information and a binocular stereo vision system. The binocular vision system indicates what the robot is looking at and is not used for locomotion. Gaze control plays an important role in human-robot interaction. This paper reports how the robot's gaze influences subjects' impressions of the robot. With a statistically significant number of robot observers, we employed psychological methods, the semantic differential method (SD), and factor analysis. This careful psychological analysis of robot impressions is the first trial in robotics. Through the experiment, we have found that the impressions mainly consist of four factors: familiarity, enjoyment, activity, and performance. The computer skills of subjects affect their impressions of the robot.
Identification of 26 new constitutionalRB1gene mutations in Spanish, Colombian, and Cuban retinoblastoma patients (2004)

Constitutional mutations in the RB1 gene predispose to retinoblastoma development. Hence genetic screening of retinoblastoma patients and relatives is important for genetic counseling purposes. In addition, RB1 gene mutation studies may help decipher the molecular mechanisms leading to tumors with different degrees of penetrance or expressivity. In the course of genetically screening of 107 hereditary and non-hereditary retinoblastoma patients (11 familiar bilateral, 4 familiar unilateral, 49 sporadic bilateral and 43 sporadic unilateral) and kindred from Spain, Colombia and Cuba, using direct PCR sequencing, we observed 45 distinct mutations and four RB1 deletions in 53 patients (9 familiar bilateral, 2 familiar unilateral, 31 sporadic bilateral and 11 sporadic unilateral). Most of these mutations (26/45, 57%) have not been reported before. In 32 patients, the predisposing mutations correspond to nonsense (mainly CpG transitions) and small insertions or deletions whose expected outcome is a truncated Rb protein that lacks the functional pockets and tail. Five single aminoacid replacements and seventeen mutations affecting splicing sites were also observed in retinoblastoma patients. Two of these sixteen mutations are of unclear pathogenic nature.
Single-Mode regime of large mode area double cladding photonic crystal fibers (2011)

In this work, carried out in the framework of the EU FP7 ALPINE project, the single-mode regime of 19-cell core DC-PCFs has been numerically analyzed by comparing different approaches. Besides the FSM, the effective index of the fundamental mode of the double cladding fiber without defect and of the first cladding mode [3] of the Yb-doped hexagonal core PCF, both computed with a full-vector modal solver based on the finite element method[4], whose magnetic field modulus distributions are shown in Fig. 1(a) and Fig 1(b) respectively, have been considered to calculate the cut-off condition of the first High-Order Mode (HOM).
Robotic Direct Inguinal Hernia Repair: To Plicate or Not to Plicate? (2021)

Postoperative seroma incidence, pain scores, and recurrence rates were similar between the 2 study groups. Multicenter studies with larger populations and higher complication counts are needed to establish the role of defect plication in RDIHR.
Site-Directed Immobilization of Proteins on Surfaces: Genetic Approaches (2002)

Improving the way by which proteins are immobilized on surfaces such that they maintain their functionality is of critical importance in medical diagnostics, bioreactors, and biosensors. Depending on the intended applications, proteins can be immobilized on surfaces by different approaches [1,2]. Immobilization through physical adsorption, though simple, usually has the problem of leakage of the immobilized proteins from the support and leads to partial denaturation of the protein. This results in a short lifetime of devices based on immobilized proteins and may even cause sample contamination, which is a great concern if these devices are to be used in vivo. Chemical immobilization methods eliminate the leakage problem by covalently tethering protein molecules to supports. Different types of functional groups, such as amino, sulfhydryl, and carboxyl groups, are frequently used in chemical immobilization of proteins to the support. Since each protein molecule usually has many of these functional groups distributed on its surface, attachment through these groups inevitably leads to a different orientation of the immobilized proteins on the support (hereafter referred to as random immobilization). In random immobilization, the active/binding site of the protein molecule may be partially or totally blocked by neighboring protein molecules or the immobilization surface due to mixed orientation of the immobilized protein (Fig. 1A). This is one of the reasons that chemicalimmobilization often leads to a significant decrease in activity/binding capacity of the immobilized protein [3,4].
REACT DX registry: Real world REACTion to atrial high rate episodes detected in implantable cardioverter-defibrillator recipients with a DX lead (2022)

The DX ICD system provides rapid and ongoing atrial rhythm monitoring such that physicians are rapidly aware of AHRE without the need for a dedicated atrial lead, but local protocols are needed to improve the response time of anti-coagulation prescription.
Dermoscopy of cutaneous leishmaniasis (2008)

Characteristic dermoscopic structures have been identified in CL. Important vascular patterns seen in melanocytic and nonmelanocytic tumours are frequently observed in this infection.
Technical Note: Efficient and accurate MRI‐only based treatment planning of the prostate using bulk density assignment through atlas‐based segmentation (2020)

Using bulk density assignment on MR images with suitable densities for bone and soft tissue results in clinically acceptable dose differences compared to dose calculated on the CT, for both atlas-based and manual bone contours. This demonstrates that an integrated MRI-only pathway utilizing a bulk density assignment for two tissue types is a feasible and robust approach for patients with prostate cancer treated with VMAT.
Generation of Multiple Mid-Infrared Wavelengths by Soliton Fission in a Photonic Crystal Fiber (2014)

Higher-order solitons are formed by coupling femtosecond pulses into the fundamental mode of a highly nonlinear silica photonic crystal fiber (HNL-SPCF) fabricated in our laboratory. It is experimentally observed that the higher-order solitons break up into a series of stable and broadband discrete fundamental solitons at the mid-infrared wavelength longer than 2000 nm through the process of soliton self-frequency shift due to the perturbations induced by the higher-order dispersive and nonlinear effects. The maximum soliton red-shift and the tunable wavelength range can be up to 1600 nm and over 400 nm, respectively. The nonlinear dynamic processes of femtosecond pulse breakup are consistent with the solution of the generalized nonlinear Schrödinger equation. This multiple stable and broadband mid-infrared wavelengths generated in such an HNL-SPCF can be used as all-fiber multiwavelength ultrashort pulse sources for multiphoton microscopy and ultrafast photonics.