gebhart/patrepeval huggingface dataset:
Biodegradable poly (lactic acid-co-glycolic acid) scaffolds as carriers for genetically-modified fibroblasts (2017)

Recent advances in gene delivery into cells allow improved therapeutic effects in gene therapy trials. To increase the bioavailability of applied cells, it is of great interest that transfected cells remain at the application site and systemic spread is minimized. In this study, we tested clinically used biodegradable poly(lactic acid-co-glycolic acid) (PLGA) scaffolds (Vicryl & Ethisorb) as transient carriers for genetically modified cells. To this aim, we used human fibroblasts and examined attachment and proliferation of untransfected cells on the scaffolds in vitro, as well as the mechanical properties of the scaffolds at four time points (1, 3, 6 and 9 days) of cultivation. Furthermore, the adherence of cells transfected with green fluorescent protein (GFP) and vascular endothelial growth factor (VEGF165) and also VEGF165 protein secretion were investigated. Our results show that human fibroblasts adhere on both types of PLGA scaffolds. However, proliferation and transgene expression capacity were higher on Ethisorb scaffolds most probably due to a different architecture of the scaffold. Additionally, cultivation of the cells on the scaffolds did not alter their biomechanical properties. The results of this investigation could be potentially exploited in therapeutic regiments with areal delivery of transiently transfected cells and may open the way for a variety of applications of cell-based gene therapy, tissue engineering and regenerative medicine.
GC-on-chip: integrated column and photoionization detector (2015)

This paper reports a unique GC-on-chip module comprising a monolithically integrated semi-packed micro separation column (μSC) and a highly sensitive micro helium discharge photoionization detector (μDPID). While semi-packed μSC with atomic layer deposited (ALD) alumina as a stationary phase provides high separation performance, the μDPID implemented for the first time in a silicon-glass architecture inherits the desirable features of being universal, non-destructive, low power consumption (1.4 mW), and responsive. The integrated chip is 1.5 cm × 3 cm in size and requires a two-mask fabrication process. Monolithic integration alleviates the need for transfer lines between the column and the detector which improves the performance of the individual components with overall reduced fabrication and implementation costs. The chip is capable of operating under the isothermal as well as temperature and flow programming conditions to achieve rapid chromatographic analysis. The chip performance was investigated with two samples: 1) a multi-analyte gas mixture consisting of eight compounds ranging from 98 °C to 174 °C in boiling point and 2) a mixture containing higher alkanes (C9-C12). Our experiments indicate that the chip is capable of providing rapid chromatographic separation and detection of these compounds (<1 min) through the optimization of flow and temperature programming conditions. The GC-on-chip demonstrated a minimum detection limit of ~10 pg which is on a par with the widely used destructive flame ionization detector (FID).
Design of a 3‐degrees of freedom platform for the stereolithography apparatus (2002)

This paper presents the design of a 3 degrees of freedom build platform for a Stereo‐lithography Apparatus machine (older model without Zephyr coating). The Pahl and Beitz methodology is used to systematically design such a component. The various steps are illustrated and the final design described. The impetus for the research is the need to reduce layering errors in layer‐based machines. The final solution allows the platform to rotate along two axes and be elevated along the Z‐axis with minimal disruption to the present set‐up.
P4–311: Intravenous immunoglobulin shifts microglial activation state toward anti‐inflammatory activity and reverses beta‐amyloid–mediated LTP inhibition (2013)

Intravenous Immunoglobulin (IVIG) has been proposed as a potential therapeutic for Alzheimer's disease (AD) and its efficacy is currently being tested in mild-to-moderate AD. Earlier studies indicated the presence of anti-A β antibodies in IVIG. This observation and the well documented anti-inflammatory properties of IVIG led to several pre-clinical and clinical studies investigating the potential role of IVIG as a therapeutic agent in AD. In this study we wanted to further characterize the mechanism of action of IVIG in AD. Therefore, we analysed the effects of IVIG on microglial phagocytosis and microglial activation state. Furthermore, we investigated the potential of IVIG to reverse A β-mediated inhibition of Long-Term Potentiation (LTP). We analysed the effects of A β and IVIG on phagocytic activity and activation state of BV-2 microglia by immunocytochemistry, FACS and Luminex. To characterize the effect of IVIG on cognition, LTP assays were performed in mouse hippocampal slice cultures. In this study we show that IVIG increased the phagocytic uptake of A β by BV-2 microglia. The immune-complexed A β was primarily endocytosed via the Fc γ receptor (FcγR). This uptake was accompanied by an upregulation of CD11b, which is indicative of an anti-inflammatory activation of microglia, an increased phagocytic activity and a reduced production of reactive oxygen species (ROS). Furthermore, we found that IVIG completely reversed A β-mediated LTP inhibition. Our results suggest that IVIG has the potential to improve a variety of pathologies linked to AD, including reversal of the inflammatory state of microglial cells which might prevent brain damage caused by oxidative stress, as well as reversal of LTP inhibition, which indicates that IVIG might also have beneficial effects on disturbed cognitive functions observed in AD patients.
Dimeric Quaternary Structure of the Prototypical Dual Specificity Phosphatase VH1 (2009)

The Vaccinia virus H1 gene product, VH1, is a dual specificity phosphatase that down-regulates the cellular antiviral response by dephosphorylating STAT1. The crystal structure of VH1, determined at 1.32 A resolution, reveals a novel dimeric quaternary structure, which exposes two active sites spaced approximately 39 A away from each other. VH1 forms a stable dimer via an extensive domain swap of the N-terminal helix (residues 1-20). In vitro, VH1 can dephosphorylate activated STAT1, in a reaction that is competed by the nuclear transport adapter importin alpha5. Interestingly, VH1 is inactive with respect to STAT1 bound to DNA, suggesting that the viral phosphatase acts predominantly on the cytoplasmic pool of activated STAT1. We propose that the dimeric quaternary structure of VH1 is essential for specific recognition of activated STAT1, which prevents its nuclear translocation, thus blocking interferon-gamma signal transduction and antiviral response.
Generalized formal models for faceted user interfaces (2009)

Faceted metadata and navigation have become major topics in library science, information retrieval and Human-Computer Interaction (HCI). This work surveys a range of extant approaches in this design space, classifying systems along several relevant dimensions. We use that survey to analyze the organization of data and its querying within faceted browsing systems. We contribute formal entity-relationship (ER) and relational data models that explain that organization and relational query models that explain systems' browsing functionality. We use these types of models since they are widely used to conceptualize data and to model back-end data stores. Their structured nature also suggests ways in which both the models and faceted systems might be extended.
Synthesis and self-assembly of giant porphyrin discsElectronic supplementary information (ESI) available: experimental procedures and characterization data, NMR- and UV/Vis-titration data. See http://www.rsc.org/suppdata/cc/b4/b401324g/ (2004)

A giant porphyrin disc (M(w)= 15 kDa) has been synthesized and its self-assembly behaviour at an interface studied by liquid STM which reveals the presence of huge domains (>400 x 400 nm2) of very well ordered and molecularly resolved columnar stacks.
Handwritten sentence recognition: from signal to syntax (2002)

This paper describes a system dedicated to online handwritten sentence recognition. The prototype is made up of two basic processors. The first controls the data acquisition, pentip trace segmentation, letter identification. The second aims at identifying and correcting the words candidates by integrating syntactic and lexical information. Sentences are parsed to list the grammatical classes of each incorrect candidates then lexical query searches for words in a lexicon according to grammatical classes. A final decision is made using a string comparison algorithm. Tests of the complete system are reported at the end for a typical writer-dependent application.
As Chemical Reviews Enters the Millennium Year (2000)

ADVERTISEMENT RETURN TO ISSUEPREVEditorialNEXTAs Chemical Reviews Enters the Millennium YearHarold HartView Author Information Michigan State UniversityCite this: Chem. Rev. 2000, 100, 1, 7–8Publication Date (Web):January 12, 2000Publication History Published online12 January 2000Published inissue 1 January 2000https://pubs.acs.org/doi/10.1021/cr990100rhttps://doi.org/10.1021/cr990100reditorialACS PublicationsCopyright © 2000 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views457Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (15 KB) Get e-AlertscloseSUBJECTS:Organic chemistry Get e-Alerts
Complications of laparoscopic inguinal hernia repair. (1997)

Laparoscopic herniorrhaphy has been gaining popularity along with other minimally invasive surgical techniques. With the sophistication of instruments, evaluation of techniques and longer follow-up of patients, the outcome of such repairs is changing. Many authors are reporting a variety of techniques, complications, and results compared to conventional surgery.
In‐field assessment of display resolution and noise: Performance evaluation of a commercial measurement system (2006)

Abstract— Two key metrics of image quality for high‐fidelity displays, including medical displays, are resolution and noise. Until now, these properties have been primarily measured in laboratory settings. For the first time, a system consisting of a CCD camera and analysis software has been made commercially available for measuring the resolution and noise of medical displays in a clinical setting. This study aimed at evaluating this new product in terms of accuracy and precision. In particular, the project involved the measurement of the modulation transfer function (MTF) and the signal‐to‐noise ratio (SNR) of two medical imaging displays, one cathode‐ray tube (CRT) display and one liquid‐crystal display (LCD) using this camera/software system. To assess the system's precision, measurements were made multiple times at the same setting. To check for accuracy, the results were compared with published values of the MTF and noise for the same displays. The performance of the system was also ascertained as a function of the focus setting of the camera. The results indicated that for the LCD, when the camera is focused within ±0.6 mm of the optimum focus setting, the MTF values lie within approximately 14% of the best focus MTF at the Nyquist frequency and 11% of the optimum overall sharpness (∫ MTF 2 df). Similar results were obtained in the horizontal and vertical directions. For the CRT, this focus produced vertical and horizontal MTF values at the Nyquist frequency within 15.2% and 61.2% of the optimum focus MTF, respectively. The figures in terms of overall sharpness were 3.0% and 0.7%. The results for the noise measurements showed a repeatability of 3% for the LCD and 13% for the CRT and a relative (but not absolute) magnitude of the noise between the two displays reflective of prior measurements. Overall, the measurement system yielded reasonably precise resolution and noise results for both display devices. The accuracy was traceable to published results only for the MTF and for relative level of display noise with differences in the absolute magnitude of noise between current and prior measurements attributed to variations in the non‐standard techniques applied for display noise measurements.
Holographic Pattern Synthesis With Modulated Substrate Integrated Waveguide Line-Source Leaky-Wave Antennas (2013)

We present the synthesis of one-dimensional (line-source) leaky-wave antennas (LWAs) in substrate integrated waveguide (SIW) technology with modulated geometry, demonstrating the capability to flexibly tailor the radiated fields pattern, both in nearand far-field regimes. The synthesis technique is inspired in holographic concepts, which are related to the existence of modulated leaky waves. A systematic design algorithm to obtain the requested modulation of the SIW width and distance between posts to synthesize the desired radiation pattern is described. Several design examples operating at 15 GHz are reported and experimentally validated, showing the power and versatility of the proposed holographic SIW technology.
Extracorporeal Membrane Oxygenation for Pediatric Cardiac Arrest (2015)

Extracorporeal cardiopulmonary resuscitation (ECPR) remains a promising treatment for pediatric patients in cardiac arrest unresponsive to traditional cardiopulmonary resuscitation. With venoarterial extracorporeal support, blood is drained from the right atrium, oxygenated through the extracorporeal circuit, and transfused back to the body, bypassing the heart and lungs. The use of artificial oxygenation and perfusion thus provides the body a period of hemodynamic stability, while allowing resolution of underlying disease processes. Survival rates for ECPR patients are higher than those for traditional cardiopulmonary resuscitation (CPR), although neurological outcomes require further investigation. The impact of duration of CPR and length of treatment with extracorporeal membrane oxygenation vary in published reports. Furthermore, current guidelines for the initiation and use of ECPR are limited and may lead to confusion about appropriate use of this support. Many ethical concerns arise with this advanced form of life support. More often than not, the dilemma is not whether to withhold ECPR, but rather when to withdraw it. Although clinicians must decide if ECPR is appropriate and when further intervention is futile, the ultimate burden of choice is left to the patient's caregivers. Offering support and guidance to the patient's family as well as the patient is essential.
Mycoplasma bovis antibody dynamics in naturally exposed dairy calves according to two diagnostic tests (2018)

We conclude that the BioX ELISA cannot be recommended for use in calves below 3 months of age. The MilA ELISA was able to detect antibodies shortly after birth (i.e. from approximately 3 weeks of age and onwards) and is therefore a more sensitive test for M. bovis exposure in young calves. Neither ELISA seemed able to differentiate between calves with arthritis and/or otitis media, and respiratory disease.
Chemical constituents and antioxidant activity of geranium wallichianum. (2009)

The study of the chemical constituents of the whole plant of Geranium wallichianum (Geraniaceae) has resulted in the isolation and characterization of six compounds. These six compounds were identified as ursolic acid (1), β-sitosterol (2), stigmasterol (3), β-sitosterol galactoside (4), herniarin (5), and 2,4,6-trihydroxyethylbenzoate (6) which were isolated for the first time from Geranium wallichianum. The above compounds were individually identified by spectroscopic analyses and comparisons with reported data. The antioxidant potential of Geranium wallichianum extracts has been investigated by DPPH radical scavenging assay and EtOAc extract was found to be most potent with IC50 19.05 ug/mL.
Fungal infections of the genitourinary tract (2019)

This chapter focuses on the pathogenesis and the spectrum of clinical manifestations of fungal urinary tract infections, with a special emphasis placed on various therapeutic approaches. Fungal infections of the urinary tract encompass a broad variety of fungi, including the endemic mycoses, Cryptococcus species, and opportunistic pathogens such as Aspergillus species. However, the overwhelming majority of fungal infections of the urinary tract are caused by Candida spp. The urinary tract is also susceptible to infection by another group of fungi, namely the endemic fungi including Histoplasma, Blastomyces, and Coccidioides spp. However, these pathogens are rarely responsible for the common clinical syndromes of urethritis, cystitis, and pyelonephritis. Instead, they occasionally cause prostatitis, epididymitis, chronic bladder inflammation or ulceration, ureteric obstruction, and chronic renal disease.
Ischemic preconditioning triggers tyrosine kinase signaling: a potential role for MAPKAP kinase 2 (1998)

Myocardial adaptation to ischemia has been shown to activate protein tyrosine kinase, potentiating activation of phospholipase D, which leads to the stimulation of mitogen-activated protein (MAP) kinases and MAP kinase-activated protein (MAPKAP) kinase 2. The present study sought to further examine the signal transduction pathway for the MAPKAP kinase 2 activation during ischemic adaptation. Isolated perfused rat hearts were adapted to ischemic stress by repeated ischemia and reperfusion. Hearts were pretreated with genistein to block tyrosine kinase, whereas SB-203580 was used to inhibit p38 MAP kinases. Western blot analysis demonstrated that p38 MAP kinase is phosphorylated during ischemic stress adaptation. Phosphorylation of p38 MAP kinase was blocked by genistein, suggesting that activation of p38 MAP kinase during ischemic adaptation is mediated by a tyrosine kinase signaling pathway. MAPKAP kinase 2 was estimated by following in vitro phosphorylation with recombinant human heat shock protein 27 as specific substrate for MAPKAP kinase 2. Again, both genistein and SB-203580 blocked the activation of MAPKAP kinase 2 during myocardial adaptation to ischemia. Immunofluorescence microscopy with anti-p38-antibody revealed that p38 MAP kinase is primarily localized in perinuclear regions. p38 MAP kinase moves to the nucleus after ischemic stress adaptation. After ischemia and reperfusion, cytoplasmic striations in the myocytes become obvious, indicating translocation of p38 MAP kinase from nucleus to cytoplasm. Corroborating these results, myocardial adaptation to ischemia improved the left ventricular functions and reduced myocardial infarction that were reversed by blocking either tyrosine kinase or p38 MAP kinase. These results demonstrate that myocardial adaptation to ischemia triggers a tyrosine kinase-regulated signaling pathway, leading to the translocation and activation of p38 MAP kinase and implicating a role for MAPKAP kinase 2.
Function smoothing with applications to VLSI layout (1999)

We present approximations to non-smooth continuous functions by differentiable functions which are parameterized by a scalar /spl beta/>0 and have convenient limit behavior as /spl beta//spl rarr/0. For standard numerical methods, this translates into a tradeoff between solution quality and speed. We show the utility of our approximations for wirelength and delay estimations used by analytical placers for VLSI layout. Our approximations lead to more "solvable" problems.
Some new results on the inverse problem for electrocardiography (1993)

The use of several mathematical methods for estimating epicardial electrocardiography (ECG) potentials from arrays of body surface has been reported in the literature; most of these methods are based on the least-squares reconstruction principle and operate in the time-space domain. The authors present some new results of the singular value decomposition (SVD)-based inverse methods using real data and a realistic high-resolution canine anatomy model. A computer-based system was designed to reconstruct an accurate canine geometric model of anatomy from 31 CT-scan cross-sections that include the internal organs and skeletal muscle inhomogeneities.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Heading-time genes of the rice varieties grown in south-west-warm region in Japan. (1991)

既知の3晩生遺伝子E1,E2およびE3に関して遺伝子型を異にする7系統(7EG系統)を検定系統に用い,我が国の栽培品種を主対象として出穂期に関与する遺伝子の解明に渚手した.本報はその第1報として,我が国西南暖地の代表的品種を対象とし,早生2品種(マンリョウ,日本晴),中生5品種(農林6号,農林8号,農林22号,金南風,中生新千本),および晩生4品種(瑞豊,アケボノ,シラヌイ,ホウヨク)の計11品種を7EG系統と交雑し,F2およびF3を用いて出穂日の分離分析を行ったものである.分析の結果,両早生品種はE1を,また中生5品種のうち3品種(農林6号,農林8号,農林22号)はE1とE2を,さらに他の中生2品種と晩生4品種はいずれもE1とE3を持つことが判明した.ただし,金南風および中生新千本は,E1,E3の外に,E1の作用を変更する1個の早生遺伝子を持つと推定された.このように,供試品種がすべてE1を有していたことから,E1は西南暖地品種にとって適応上不可欠の遺伝子であると考えられた.また,どの交雑組合せにおいても,E1,E2,E3および上記早生遺伝子以外の主働遺伝子による分離が認められなかったことから,西南暖地品種間にみられる早晩性の変異はE2,E3および1早生遺伝子によってほぼ説明できると結論された.
Strategic Railway Construction in Manchuria (1935)

Other| November 06 1935 Strategic Railway Construction in Manchuria J. R. S. J. R. S. Search for other works by this author on: This Site PubMed Google Scholar Far Eastern Survey (1935) 4 (22): 178–179. https://doi.org/10.2307/3022856 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation J. R. S.; Strategic Railway Construction in Manchuria. Far Eastern Survey 6 November 1935; 4 (22): 178–179. doi: https://doi.org/10.2307/3022856 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAsian Survey Search This content is only available via PDF. Article PDF first page preview Close Modal You do not currently have access to this content.