gebhart/patrepeval huggingface dataset:
kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylation (2021)

Abstract Anterograde melanosome transport is essential for adaptive skin tanning response. However, the molecular components involved, their interplay and regulation by external cues in melanosome transport remain under-explored. Silencing of kinesin motors revealed that several members including the established KIF5B and a novel candidate KIF1B, mediate melanosome movement. The camouflage behaviour of zebrafish embryos induced by incident light or α -MSH requires kif1b, suggesting a conserved melanosome transport machinery across vertebrates. Interestingly, the peri-nuclear melanosome accumulation upon kinesin knockdown is recapitulated by the silencing of autophagy effector MAP1LC3B (LC3B). Pull-down assays identified KIF1B, but not KIF5B, to be the LC3B-associated kinesin. LC3B binds the adapter SKIP via its LIR docking region that is proximal to Thr12 residue, a site for phosphorylation by Protein Kinase A. We demonstrate that phosphorylation of LC3B at Thr12 is stimulated by α-MSH, which potentiates the anterograde melanosome transport. Thereby, our study, identifies a novel kinesin motor KIF1B for melanosome movement and establishes LC3B as the key molecular component that facilitates α-MSH responsive mobilization of melanosomes. Key Highlights Kinesin screen reveals non-redundant use of KIF5B, KIF1B motors for melanosome transport kif1b is required for camouflage response in zebrafish and melanosome movement in mammals N-terminal region of LC3B interacts with adapter SKIP and couples kinesin KIF1B α -MSH activates PKA-mediated phosphorylation of LC3B to potentiate anterograde movement Significance Melanosomes are lysosome related organelles containing melanin pigment, that are synthesized in melanocytes and transferred to the recipient keratinocytes of skin. This involves long range melanosome movement within melanocytes to reach cell periphery for the transfer to follow. Physiologically, UV protection involves local secretion of melanocyte stimulating hormone ( α -MSH) that acts on melanocytes to promote skin tanning response. Herein, we investigate the components involved in this process and establish that the melanosome movement is dynamically controlled by α -MSH through phosphorylation of LC3B. These findings establish the mechanism behind the rapid distribution of melanosomes during tanning response and provide opportunity to intervene for sun protection.
Analysis of Clinical Variations in Asthma Care Documented in Electronic Health Records Between Staff and Resident Physicians (2017)

Clinical documentation using free text to describe a patient's medical status is an essential component of electronic health records (EHRs), and the quality of information in documents plays a critical role in clinical practice and translational research. Physicians are the primary creators of EHRs, but their clinical practices vary substantially, resulting in variations in clinical documentation. These variations can represent a source for potential bias in clinical outcomes and downstream applications using EHRs. Asthma is one example, presenting an inconsistent ascertainment process and criteria. A recent study revealed that resident physicians' knowledge of asthma diagnosis and management is relatively limited. In this study, we examined clinical documentation variations in asthma care between staff and resident physicians using individual words, topics, and asthma-related concepts in EHR clinical narratives. Additionally, we discuss potential biases in building an informatics model and further compare asthma diagnosis and outcomes between two physician groups.
Differences in Abundances of Total Vibrio spp., V. vulnificus, and V. parahaemolyticus in Clams and Oysters in North Carolina (2016)

This study shows that clams are a potential source of infection in North Carolina, especially for V. parahaemolyticus These findings also highlight the need for clam-specific environmental research to develop accurate Vibrio abundance models and to broaden the ecological understanding of clam-Vibrio interactions. This is especially relevant as foodborne Vibrio infections from clams are being reported.
Duurzaam gewasproductiesysteem voor de toekomst (1998)

Korte presentatie van een concept dat aan de Duurzame Technologische Ontwikkeling (DTO) moet kunnen voldoen. Omdat het een ontwerp voor de toekomst betreft wordt het ook wel afgekort tot 'KAS 2020'
Simultaneous Analysis and Efficient Separation of Anabolic Androgenic Steroids in Dietary Supplement by a Validated HPTLC Method (2022)

In the present study, the fast, cheap, and simple HPTLC method could be used for the multi-residue analysis of ten anabolic androgenic steroids in sports supplements.
Abstract and Discussion: Supply of and Demand for Technician and Professional Engineers (1967-82) (1981)

This paper describes the fourth national survey carried out at the University of Canterbury to investigate supply of and demand for both professional and technician engineers over a six year period from March 1976 to March 1982. Results from this survey which was carried out in 1979 have been combined with those from the earlier surveys of 1970, 1973, and 1976. Results are classified according to type of employer and branch of engineering and information is given on technician/ engineer ratios. The investigation shows that supply is catching up on demand and vacancies may be nominal only by March 1982 while there will be a surplus of some types of engineers and a shortage of others. The expansion in numbers of and demand for technician engineers has again been greater than for professional engineers, but the overall ratio of technician to professional engineers was no better in 1979 than it was in 1973.
Motion control of magnetized Tetrahymena pyriformis cells by a magnetic field with Model Predictive Control (2012)

This paper presents the Model Predictive Control (MPC) of magnetized Tetrahymena pyriformis (T. pyriformis) using a magnetic field. The magnetized T. pyriformis are generated by feeding spherical iron oxide particles into the cells. Using an external magnetic field, we change the movement direction of the cell, but the speed of the cell remains constant regardless of the strength of the external magnetic field. The contributions of this paper are threefold. First, the discrete-time plant model of the magnetized cell is generated using the least-squares method. Second, using the model of each cell, they are controlled to follow a reference track by an external magnetic field with MPC. Third, by using a predictor-like scheme to execute the plant input before the measurement of the cell position, we successfully solve the image-processing delay problem in the feedback system. In our results, we show three comparisons between different control schemes and an initial tracking to prove the effectiveness of the control approach.
A Theoretical Comparison of Texture Algorithms (1980)

An evaluation of the ability of four texture analysis algorithms to perform automatic texture discrimination will be described. The algorithms which will be examined are the spatial gray level dependence method (SGLDM), the gray level run length method (GLRLM), the gray level difference method (GLDM), and the power spectral method (PSM). The evaluation procedure employed does not depend on the set of features used with each algorithm or the pattern recognition scheme. Rather, what is examined is the amount of texturecontext information contained in the spatial gray level dependence matrices, the gray level run length matrices, the gray level difference density functions, and the power spectrum. The comparison will be performed in two steps. First, only Markov generated textures will be considered. The Markov textures employed are similar to the ones used by perceptual psychologist B. Julesz in his investigations of human texture perception. These Markov textures provide a convenient mechanism for generating certain example texture pairs which are important in the analysis process. In the second part of the analysis the results obtained by considering only Markov textures will be extended to all textures which can be represented by translation stationary random fields of order two. This generalization clearly includes a much broader class of textures than Markovian ones. The results obtained indicate that the SGLDM is the most powerful algorithm of the four considered, and that the GLDM is more powerful than the PSM.
Hypertension and the Interior Ion-milieu. Can we Overcome Hypertension with Regulated Breathing? (2022)

The author presents a new model for age-associated diseases through a hypothesized pathogenesis of hypertension. Preservation or restoration of the constancy of each original cell-specific intracellular ion-pattern is essential for upholding cellular identity and integrity. The ageing background is intracellular acidosis: low-grade respiratory acidosis elevates the intracellular HCO 3 -/Cl - gradient and induces metabolic syndrome, while low-grade metabolic acidosis causes exhausting buffer syndrome (EBS) with a decreased intracellular HCO 3 -/Cl -rate. The former elicits higher aldosterone levels to restore the original ion-pattern by NHE-1 (natrium-hydrogen exchange) mechanism located in the membrane of vascular smooth muscle cells (VSMCs) and by retaining NaCl.
In Vitro Passive Sensitization of Guinea Pig, Rhesus Monkey and Human Bladders As a Model of Noninfectious Cystitis (1994)

Studies of human bladder inflammation have been limited to examination of urine, bladder biopsy, or examination of autopsy material. We have developed an in vitro bladder passive sensitization technique which can measure type I responses of isolated human bladder tissue. We have compared these results using human tissue to those obtained with bladder tissue from guinea pigs and Rhesus monkeys. In our studies, bladder tissue was passively sensitized in vitro for 20 hours with immunoglobulin-containing serum. Subsequent antigen challenge of the passively sensitized tissue resulted in a time-dependent contraction that was accompanied by tissue histamine release. Contractions of guinea pig, monkey and human bladder tissue reached 79%, 100% and 78% of the maximal contraction induced by potassium chloride. In contrast, adjacent strips of unsensitized tissue had no detectable response to antigen challenge. The responses were reduced in the presence of histamine H1 receptor blockade with pyrilamine and abolished in the presence of a concomitant blockade of leukotriene synthesis with nordihydroguaiaretic acid (NDGA). Blockade of cyclooxygenase activity with indomethacin increased the contraction of the sensitized guinea pig bladder in response to antigen challenge. These findings demonstrate that in vitro passive sensitization of human bladder tissue can be used to investigate basic mechanisms of noninfectious bladder inflammation in humans.
Age-related changes in the distribution of Nav1.1 and Nav1.2 in rat cerebellum (2003)

Modification of sodium channel availability and behavior is obviously a good candidate for alteration of action potential observed during aging. In the present study, we revealed age-related alterations in the expression of voltage-gated Na+ (Na(v)) channel in rat cerebellum by immunohistochemistry. In the cerebellar cortex of aged rats, Na(v)1.1 immunoreactivity in Purkinje cell bodies was highly increased, whereas granule cells showed lower staining intensity. In the cerebellar nuclei of aged rats, Na(v)1.1 and Na(v)1.2 expression was specifically increased in the cerebellar output neurons, which was confirmed by image analysis. The first demonstration of age-related changes in Na(v) channel expression contributes to our understanding of the mechanisms responsible for alteration in synaptic transmission during aging.
Detailed spectral analysis of the 260 ksec XMM-Newton data of 1E1207.4-5209 and significance of a 2.1 keV absorption feature (2004)

We have done a reanalysis of the 260 ksec XMM-Newton observation of 1E1207.4-5209 described in Bignami et al. (2003) and De Luca et al. (2004). We find no evidence for a third or fourth feature in the spectrum, which had been interpreted as higher harmonics of a cyclotron line. Only the two broad absorption features previously reported are statistically significant (Sanwal et al. 2002, Hailey &amp; Mori 2002). We identify the origins of the overestimate of the significance of the third and fourth spectral lines. As first pointed out by Protassov et al. (2002) the F-statistic in the case of a line search cannot be described by the standard F-distribution even asymptotically, as assumed in the previous analyses. Therefore no valid conclusions can be drawn from these analyses. We calculate the finite statistics F-distribution and show that with this correct distribution the third and fourth lines are not significant. We confirm this with two additional statistical tests, one line model dependent and the other independent. Our continuum fits have significantly lower chi^2/nu (chi^2/nu ~ 1) than earlier work, and this is true for a much broader range of physically plausible models than were previously investigated. This further reduced the significance of the extra lines. We explicitly fit the insignificant residuals around 2.1 keV. For those models where the weak residuals are strongest, we find them at an instrument Au-M edge, and not at a statistically distinct energy, in contrast to earlier studies. This is true not only for the model favored in earlier investigations, but for all reasonable continuum models.
Quiet Color (2008)

Book Review| January 01 2008 Quiet Color JEFF WALL ART INSTITUTE OF CHICAGOCHICAGO, ILLINOIS JUNE 29–SEPTEMBER 23, 2007 Kristin Miller Kristin Miller KRISTIN MILLER is an artist, writer, educator, and Visual Studies Workshop graduate. She teaches art and art history at Palm Beach Community College and works at Red Dot Contemporary in West Palm Beach, Florida. Search for other works by this author on: This Site PubMed Google Scholar Afterimage (2008) 35 (4): 20–21. https://doi.org/10.1525/aft.2008.35.4.20 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Kristin Miller; Quiet Color. Afterimage 1 January 2008; 35 (4): 20–21. doi: https://doi.org/10.1525/aft.2008.35.4.20 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAfterimage Search This content is only available via PDF. © 2008 Afterimage/Visual Studies Workshop, unless otherwise noted. Reprints require written permission and acknowledgement of previous publication in Afterimage.2008 Article PDF first page preview Close Modal You do not currently have access to this content.
The MicroRNA and MessengerRNA Profile of the RNA-Induced Silencing Complex in Human Primary Astrocyte and Astrocytoma Cells (2010)

The observation that miR-34a and miR-195 levels were increased in the RISC of U-87 astrocytoma cells suggests an oncogenic role for these miRNAs. Differential regulation of mRNAs by specific miRNAs is evidenced by the observation that three miR34a-targeted mRNAs and two miR-195-targeted mRNAs were downregulated while one miR-195-targeted mRNA was upregulated. Biological pathway analysis of RISC mRNA components suggests that the RISC plays a pivotal role in malignancy and other conditions. This study points to the importance of the RISC and ultimately GW/P body composition and function in miRNA and mRNA deregulation in astrocytoma cells and possibly in other malignancies.
Tags to Track Illicit Uranium and Plutonium (2007)

With the expansion of nuclear power, it is essential to avoid nuclear materials from falling into the hands of rogue nations, terrorists, and other opportunists. This paper examines the idea of detection and attribution tags for nuclear materials. For a detection tag, it is proposed to add small amounts [about one part per billion (ppb)] of {sup 232}U to enriched uranium to brighten its radioactive signature. Enriched uranium would then be as detectable as plutonium and thus increase the likelihood of intercepting illicit enriched uranium. The use of rare earth oxide elements is proposed as a new type of 'attribution' tag for uranium and thorium from mills, uranium and plutonium fuels, and other nuclear materials. Rare earth oxides are chosen because they are chemically compatible with the fuel cycle, can survive high-temperature processing operations in fuel fabrication, and can be chosen to have minimal neutronic impact within the nuclear reactor core. The mixture of rare earths and/or rare earth isotopes provides a unique 'bar code' for each tag. If illicit nuclear materials are recovered, the attribution tag can identify the source and lot of nuclear material, and thus help police reduce the possible number of suspects in the diversion ofmore » nuclear materials based on who had access. (authors)« less
Optical Characterization of Marine Microorganisms and Other Biogenic Particles (1997)

Abstract : The long-term goal of this research is to develop the base of knowledge necessary to: (i) understand the magnitudes and variability of the optical properties in the upper ocean; (ii) predict spectral absorption and scattering by seawater as well as underwater light fields and water-leaving radiant fluxes, given the types and concentration of suspended particles. My specific objective during the past year was to expand a database of single-particle optical properties for various types of marine microorganisms.
Feature selection for appearance-based vehicle tracking in geospatial video (2013)

Current video tracking systems often employ a rich set of intensity, edge, texture, shape and object level features combined with descriptors for appearance modeling. This approach increases tracker robustness but is compu- tationally expensive for realtime applications and localization accuracy can be adversely affected by including distracting features in the feature fusion or object classification processes. This paper explores offline feature subset selection using a filter-based evaluation approach for video tracking to reduce the dimensionality of the feature space and to discover relevant representative lower dimensional subspaces for online tracking. We com- pare the performance of the exhaustive FOCUS algorithm to the sequential heuristic SFFS, SFS and RELIEF feature selection methods. Experiments show that using offline feature selection reduces computational complex- ity, improves feature fusion and is expected to translate into better online tracking performance. Overall SFFS and SFS perform very well, close to the optimum determined by FOCUS, but RELIEF does not work as well for feature selection in the context of appearance-based object tracking.
Isolation and structures of trichilins, antifeedants against the Southern army worm (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation and structures of trichilins, antifeedants against the Southern army wormMunehiro Nakatani, John C. James, and Koji NakanishiCite this: J. Am. Chem. Soc. 1981, 103, 5, 1228–1230Publication Date (Print):March 1, 1981Publication History Published online1 May 2002Published inissue 1 March 1981https://pubs.acs.org/doi/10.1021/ja00395a046https://doi.org/10.1021/ja00395a046research-articleACS PublicationsRequest reuse permissionsArticle Views278Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Modes of Action of Acarbose Hydrolysis and Transglycosylation Catalyzed by a Thermostable Maltogenic Amylase, the Gene for Which Was Cloned from a Thermus Strain (1999)

A maltogenic amylase gene was cloned in Escherichia coli from a gram-negative thermophilic bacterium, Thermus strain IM6501. The gene encoded an enzyme (ThMA) with a molecular mass of 68 kDa which was expressed by the expression vector p6xHis119. The optimal temperature of ThMA was 60 degrees C, which was higher than those of other maltogenic amylases reported so far. Thermal inactivation kinetic analysis of ThMA indicated that it was stabilized in the presence of 10 mM EDTA. ThMA harbored both hydrolysis and transglycosylation activities. It hydrolyzed beta-cyclodextrin and starch mainly to maltose and pullulan to panose. ThMA not only hydrolyzed acarbose, an amylase inhibitor, to glucose and pseudotrisaccharide (PTS) but also transferred PTS to 17 sugar acceptors, including glucose, fructose, maltose, cellobiose, etc. Structural analysis of acarbose transfer products by using methylation, thin-layer chromatography, high-performance ion chromatography, and nuclear magnetic resonance indicated that PTS was transferred primarily to the C-6 of the acceptors and at lower degrees to the C-3 and/or C-4. The transglycosylation of sugar to methyl-alpha-D-glucopyranoside by forming an alpha-(1,3)-glycosidic linkage was demonstrated for the first time by using acarbose and ThMA. Kinetic analysis of the acarbose transfer products showed that the C-4 transfer product formed most rapidly but readily hydrolyzed, while the C-6 transfer product was stable and accumulated in the reaction mixture as the main product.
A Switchable Folded-Cascode OTA without Transmission Gates in the Signal Path (2008)

This paper presents a folded-cascode operational transconductance amplifier (OTA), which allows direct, inverted or no signal transconductance without the use of transmission gates in the signal path. Therefore, the OTA is not only an active filter element, but can also replace the switching network otherwise necessary in field programmable analog arrays. It achieves a maximum gain-bandwidth of 186 MHz in a 0.13 ¿m 1.2 V CMOS technology consuming 1.1 mW. This paper presents design methodology and measurements of linearity, process variation, and signal blocking.
Effect of ormeloxifene, a nonsteroidal once-a-week oral contraceptive, on systemic hemodynamics in adult female rats (2011)

The findings demonstrate comparable pharmacological profile of ormeloxifene and raloxifene on short-term administration to rats. Based on changes observed in the ECG (R wave), long-term studies may lead to justifiable comparison of beneficial and harmful effects of ormeloxifene and raloxifene in relation to cardiovascular effects.
Optical harmonic generation in liquid crystals (1970)

The non-linear optical properties of several cholesteric and nematic liquid crystals are studied in laser experiments on generation of secondand third-harmonic radiation. No secondharmonic is observed in any of the liquid crystal mesomorphic phases, indicating that the local molecular ordering in these phases is centrosymmetric. The generality of this result to datediminishes the potential of liquid crystals as nonlinear materials. Large intensity changes ofthird harmonic are observed at several phase transitions, and their possible origins are discussed. Tentative results are presented on experiments to utilize the high optical rotatory power of the cholesteric phase to obtain phase matching in third-harmonic generation.