gebhart/patrepeval huggingface dataset:
p16INK4A adenovirus-mediated gene therapy for human head and neck squamous cell cancer. (1998)

Inactivation of the tumor suppressor gene p16INK4A is the most common genetic alteration in human head and neck squamous cell cancer (HNSCC), making it an ideal target for gene replacement. We constructed a replication-defective, recombinant adenovirus capable of directing a high level of p16INK4A protein expression (Ad5-p16) to investigate its benefit in treating HNSCC. Initial in vitro experiments in four human HNSCC cell lines demonstrated that Ad5-p16 treatment significantly inhibits cell growth with up to 96% efficiency. Flow cytometric analysis showed that Ad5-p16 induced a maximum G1-S cell cycle arrest of 90%. Subsequent studies in a nude mouse model demonstrated that Ad5-p16 treatment significantly reduced (cell line 011) or stabilized (cell line 012) established tumors when compared with control treatments (P < 0.008). These results demonstrate for the first time a significant antitumor effect of Ad5-p16 against human HNSCC in vivo and support the potential application of Ad5-p16 to treat locally advanced, unresectable, or metastatic head and neck cancer, as well as microscopic residual disease after surgical resection.
Abstract 5330: Cell identity, count, and viability for critical quality attributes using the Cellaca PLX image cytometer (2023)

Abstract Cell and Gene therapy is one of the fastest growing fields for cancer therapeutics that heavily relies on robust and consistent instrumentations and technologies to verify and validate the critical quality attributes (CQA) that are fit-for-purpose. Some of the key parameters required for satisfying CMC (Chemistry Manufacturing and Controls) criteria standards for cellular therapeutic products are cell identity, count, viability, purity, potency, stability, and microbiological quality, which may be routinely performed using flow cytometry. Some of these critical attributes can be exceedingly trivial for flow cytometric analysis, which can be complex, requires a dedicated user, and may carry a high maintenance cost. In the past decade, affordable image cytometry has been developed for cell characterization and cell-based assays but has not demonstrated the sensitivity required for visualization and analysis of surface markers, cell health, and viability. In this work, we demonstrate the capability of the newly developed Cellaca PLX image cytometry system for population analysis of surface markers, viability of fluorescent protein expressing cells, and cell health in comparison to flow cytometry. For immunophenotyping assays, PBMCs were stained with anti-human CD3/CD4/CD8 antibodies with Hoechst. Additionally, viability detection of GFP and RFP-containing cell lines was performed by staining with RubyDead and Hoechst. Finally, apoptosis was induced in Jurkat cells with staurosporine and stained with Caspase 3-488, RubyDead, and Hoechst. All assays were imaged and analyzed using the Cellaca PLX image cytometer, and the data generated were compared directly to the CytoFlex flow cytometer. Results show similar percentages of surface marker populations: CD3 (76% and 81%, PLX and CytoFlex, respectively), CD4 (43% and 42%), and CD8 (15% and 17%). RFP and GFP-containing cells showed comparable viabilities on Cellaca PLX and CytoFlex, and the population percentages of Hoechst positive cells stained with Caspase 3 and RubyDead were within 5% of each other. Our experiments demonstrate that the Cellaca PLX can be of significant value to the Cell and Gene Therapy communities providing novel image cytometry methods which may satisfy several CMC criteria including high-throughput, high sensitivity, and low maintenance. Citation Format: Carolina Franco Nitta, Mackenzie Pierce, Aiyana Parker, Sopaul Hem, Timothy Smith, Surbhi Ratnani, Marek Dobrowolski, Srinivas Gundimeda, Bo Lin, Dmitry Kuksin, Leo L. Chan. Cell identity, count, and viability for critical quality attributes using the Cellaca PLX image cytometer. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5330.
The biological activity of new tuftsin derivatives--induction of phagocytosis. (2007)

Phagocytosis plays a crucial role in a host defense against invading microorganisms. This process can be induced by many phagocytosis stimulating factors. One of them is an endogenous tetrapeptide - tuftsin that occurs in the blood of mammals including human beings. Tuftsin is capable of potentiating granulocyte and macrophage functions such as: phagocytosis, motility, and chemotaxis as well as bactericidal and tumoricidal activity. The other particle able to induce phagocytosis is muramyl dipeptide (MDP), the smallest synthetic glycopeptide of bacterial origin that possesses an immunogenic activity. MDP is known to affect most functions of macrophages. Phagocytosis stimulating properties of a new group of tuftsin and MDP analogues (one tuftsin analogue and four conjugates of tuftsin/retro tuftsin and muramyl dipeptide or nor-muramyl dipeptide) were tested. The results of the study show that all of the examined conjugates are able to generate oxidative burst. The most promising analogues proved to be kd6 and kd7.
Food Waste Composting Institutional and Industrial Applications (2009)

Fruits, vegetables, dairy products, grains, bread, unbleached paper napkins, coffee filters, eggshells, meats and newspaper can be composted. If it can be eaten or grown in a field or garden, it can be composted. Items that cannot be composted include plastics, grease, glass, and metals -including plastic utensils, condiment packages, plastic wrap, plastic bags, foil, silverware, drinking straws, bottles, polystyrene or chemicals. Items such as red meat, bones and small amounts of paper are acceptable, but they take longer to decompose. Add red meat and bones to only a well-controlled compost pile to avoid attracting vermin, pests and insects to partially decomposed meat scraps.
Estimated land cover properties for hydrologic modeling in Arequipa, Peru (2020)

&lt;p&gt;This dataset contains estimated land cover properties required for the Soil and Water Assessment Tool (SWAT) hydrologic modeling in the Arequipa department of Peru. In a two-step process, first, SWAT equivalent land covers to local land cover types were identified and their properties were used as the base for the database development. Then parameters that control the seasonal growth cycle were modified to better reflect the characteristics of local vegetation. The resulting database and raster land cover map are provided in a format suitable for SWAT model development.&lt;/p&gt;
Frozen Blood and Transfusion-Transmitted Hepatitis C Virus (1993)

The purpose of the present study was to evaluate the impact of frozen red cell transfusion on the transmission of hepatitis C virus (HCV) before the introduction of blood donor screening. Anti-HCV antibodies were detected in 59 patients with sickle-cell disease who required chronic transfusions and had exclusively received frozen red blood cells (RBC). The files were reviewed for clinical signs of chronic hepatitis C. Anti-HCV antibodies were detected in 2 adult patients; both also had clinical evidence of HCV infection. No other patient showed signs of acute or chronic HCV hepatitis. In a control group of 28 patients who had received nonfrozen RBC transfusions, the prevalence of anti-HCV antibodies was 25%. So, our study seems to indicate that the use of frozen RBC had reduced the risk of HCV contamination.
Maximising the value of irrigation through improved use of soil resources and sensor technology (2019)

This paper presents a case-study approach focussing on variability of soils, soil physical properties, and how the use of proximal sensor surveys and soil moisture monitoring can be used to improve irrigation management at fine spatial scales (&lt;10 m). Proximal sensor survey data have been used to map soil variability and statistically derive management zones, which are then correlated with S-map siblings using soil moisture release curves. At the first case study site, soil moisture monitoring of these management zones showed the poorly drained soil had wetter conditions than the other zones, which is likely to have been a factor contributing to reduced barley yield. Less irrigation could therefore have been applied to the poorly drained soil, with a saving in cost and yield penalty. In the second case study, we provide an overview of research focussing on practical applications of near real-time soil moisture monitoring and visualisation through smart phone apps, enabling new irrigation software and hardware to be matched to specific farm circumstances, so soils and crops can be managed to reduce water and nutrient losses.
Contrast Ultrasound Dispersion Imaging (CUDI) (2012)

In cats anesthetized with alpha-chloralose, different populations of motor units in soleus, plantaris, and medial gastrocnemius muscles were stimulated in rotation through surgically divided groups of ventral roots. As a result, smooth muscular contractions were obtained at low frequencies of rotary stimulation, and maximal tetanic tensions were induced at frequencies within the upper range of voluntary contractions. The frequencies necessary to induce maximal tetanic contractions were lower for slow-twitch muscles than for fast-twitch muscles. It is speculated that the primary benefit resulting from asynchronous stimulation of the muscles is in the engagement of the tendon, which permits motor units to exert their tension without having to overcome a major portion of the elastic damping.
An introduction to MPEG-4 audio lossless coding (2004)

Lossless coding is to become the latest extension of the MPEG-4 audio standard. In response to a call for proposals, many companies have submitted lossless audio codecs for evaluation. The codec of the Technical University of Berlin was chosen as reference model for MPEG-4 audio lossless coding (ALS), attaining working draft status in July 2003. The encoder is based on linear prediction, which enables high compression even with moderate complexity, while the corresponding decoder is straightforward. The paper describes the basic elements of the codec, points out envisaged applications, and gives an outline of the standardization process.
Electrical Resistance Type Humidity Sensor Using Ionic Copolymers (1986)

An electrical resistance type humidity sensor using ionic copolymers has been studied. A humidity sensitive layer of copolymer film was placed on a pair of combshaped electrodes on a ceramic plate. The ionic copolymers were synthesized from ionic and nonionic monomers in aqueous media.The electrical resistance of ionic copolymers depended significantly not only on relative humidity, but also on mole fraction of ionic monomer to nonionic monomer. The resistances decreased exponentially with an increase in relative humidity, and they increased with decreasing of mole fraction of ionic monomer in copolymers.In the sorption process, the humidity sensor responded quicker than in the desorption process. The difference of response in the desorption process remarkably depended on the kind of the copolymers. The response became quicker according to the order shown by hydrophobicity of ionic monomers used. Its cause is analyzed that the diffusion coefficient of water became larger. as loosing the bind between the water molecules adsorbed by copolymers and ionic groups.The humidity-resistance characteristics of the sensor changed when it was exposed to high temperature atmosphere. The life time of the sensors depended significantly on the kind of ionic copolymers and temperature. The life time decreased exponentially with temperature rise.
Natural Killer Cell Lymphoma in a Pediatric Patient With Inflammatory Bowel Disease (2010)

Tumor necrosis factor α (TNF-α) antibody agents are an effective therapy for the treatment of inflammatory bowel disease (IBD); however, because of the potential for immune suppression with these drugs, TNF-α antibody agents can increase the risk of malignancy. We report here the case of an 11-year-old boy who presented with bowel obstruction. He also had a history of periodic fever, aphthous stomatitis, and cervical adenitis (PFAPA). Intestinal inflammation continued and impaired his quality of life; he was diagnosed with IBD of an undetermined type (IBD-U). Symptoms improved with infliximab, but he developed elevated transaminase levels with hepatosplenomegaly 1 year after scheduled infusions. Skin biopsy revealed an atypical lymphoid infiltrate consistent with an Epstein-Barr virus (EBV)-positive natural killer (NK)/T-cell lymphoma with associated hemophagocytic lymphohistiocytosis. Bone marrow biopsy revealed a similar EBV-positive lymphoid infiltrate consistent with an NK/T-cell lymphoma. EBV-positive tissue was present in gastrointestinal biopsies. Flow-cytometric analysis revealed an atypical, clonal NK-cell population, and biopsy specimens from several tissue sites tested positive for CD3, CD56, and CD30. The patient died soon after the diagnosis was made. This patient developed an EBV-driven malignancy while receiving infliximab. All patients with IBD who receive infliximab should be monitored for malignancy, especially young patients. This case underscores the need for future studies to better understand the biology of lymphoproliferative disorders.
Field Evaluation of Lethal Ovitrap Against Dengue Vectors (2003)

Abstract : The goal of this study was to determine the efficacy of the lethal ovitrap (LO) an inexpensive, environmentally sound device designed to be integrated with existing control programs in the suppression of dengue mosquito vector populations in Barbados, East Timor and India.
Loop Transmission Aspects of ISDN Basic Access (1986)

The status of the formulation of standards for loop transmission to provide Basic Access in the North American loop plant is reviewed. The impact of loop plant bridged taps on performance is discussed, the reasons for preferring echo canceller technology over time compression multiplexing are reviewed, and issues involved in the specification of echo canceller requirements are discussed. This includes line code selection and receiver design, including linear and decision feedback equalizer design. Some of the possible methods of timing recovery are reviewed, and future directions for the evolution of Basic loop access are considered.
Restrictive usage of monoclonal immunoglobulin λ light chain germline in POEMS syndrome (2008)

POEMS syndrome is a rare plasma cell disorder characterized by peripheral neuropathy, monoclonal gammopathy, and high levels of serum vascular endothelial growth factor, the pathogenesis of which remains unclear. A unique feature of this syndrome is that the proliferating monoclonal plasma cells are essentially lambda-restricted. Here we determined complete nucleotide sequences of monoclonal immunoglobulin lambda light chain (IGL) variable regions in 11 patients with POEMS syndrome. The V-region of the Ig lambda gene of all 11 patients was restricted to the V lambda 1 subfamily. Searching for homologies with IGL germlines revealed that 2 germlines, IGLV1-44*01 (9/11) and IGLV1-40*01 (2/10), were identified, with an average homology of 91.1%. The IGLJ3*02 gene was used in 11 of 11 re-arrangements with an average homology of 92.2%. These data suggest that the highly restricted use of IGL V lambda 1 germlines plays an important role in the pathogenesis of POEMS syndrome.
Working Memory Impairment in Children With Developmental Dyslexia: Is it Just a Phonological Deficity? (2011)

Although reduced verbal span is well documented in individuals with developmental dyslexia, the existing data on visual-spatial span are inconclusive. The aim of the present study was to ascertain whether the working memory deficit in developmental dyslexia is confined to verbal material or whether it also involves visual-object and visual-spatial information. Results document deficits on span tasks tapping verbal, visual-spatial, and visual-object working memory in dyslexic children and indicate that the working memory deficit in developmental dyslexia is not limited to dysfunction of phonological components but also involves visual-object and visual-spatial information.
«Le panneau de Cortessem», alias Scènes de la vie de la Vierge des Musées royaux des Beaux-Arts de Belgique, pré-eyckien ou lombard ? (2005)

Colman Pierre. «Le panneau de Cortessem», alias Scènes de la vie de la Vierge des Musées royaux des Beaux-Arts de Belgique, pré-eyckien ou lombard ?. In: Bulletin de la Classe des Beaux-Arts, tome 16, n°1-6, 2005. pp. 21-58.
Design and Testing of an Integrated Circuit for Multi-Electrode Neural Recording (2007)

The authors have developed a single-chip neural recording system with wireless power delivery and telemetry. The 0.5-mum CMOS IC is designed to be bonded to the back of a 100-channel Utah electrode array. A pad near each amplifier allows connection of the chip to the MEMS electrode array. The complete integrated neural interface receives power wirelessly through a 2.64-MHz inductive link. A clock, regulated supply, and commands are derived from the power signal .The neural amplifiers each have a gain of 60 dB. A 10-bit charge-redistribution ADC is used to digitize the signal from one amplifier selected with an analog MUX. Digitizing all channels simultaneously would generate prohibitively high data rates; therefore, the authors perform data reduction by incorporating one-bit "spike detectors" into each amplifier. Neural data is transmitted off chip using an integrated 433-MHz FSK transmitter. The chip measures 4.7 times 5.9 mm and consumes 13.5 mW of power
Ischemia-Reperfusion Lung Injury Is Prevented by Apocynin, a Novel Inhibitor of Leukocyte NADPH Oxidase (1999)

Ischemia and reperfusion (IR) of isolated sheep lungs caused polymorphonuclear leukocyte (PMN) sequestration, pulmonary hypertension secondary to thromboxane (TX), edema, and increased vascular permeability.1Pearse DB Sylvester JT Vascular injury in isolated sheep lungs: role of ischemia, extracorporeal perfusion, and oxygen.Am J Respir Crit Care Med. 1996; 153: 196-202Crossref PubMed Scopus (17) Google Scholar PMN depletion attenuated this injury, suggesting that PMN-derived O2 radicals were involved.2Pearse DB Brower RG Adkinson Jr, NF et al.Spontaneous injury in isolated sheep lungs: role of perfusate leukocytes and platelets.J Appl Physiol. 1989; 66: 1287-1296Crossref PubMed Scopus (19) Google Scholar Apocynin (Apo) is a methoxy-substituted catechol that requires activation by myeloperoxidase to inhibit the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and thus prevents O2− production from activated PMN leukocytes. To determine the effect of Apo in IR injury, we subjected 20 sheep lungs to 30 min of ischemia and 180 min of reperfusion with blood. We injected 3 mM, 0.3 mM, 0.03 mM Apo, or diluent (n = 5 each) into the pulmonary artery early in ischemia and the reservoir before reperfusion. Peak pulmonary artery pressure, peak perfusate TXB2 concentration, and the reflection coefficient for albumin were measured (Table 1) . The ability of Apo to inhibit in vitro O2− production from zymosan-activated sheep PMN leukocytes was also assessed (n = 3).Table 1MeasurementsGroupReflection Coefficient for AlbuminPeak Pulmonary Artery Pressure, mm HgTXB2, ng/mLO2 InhibitionControl0.47 ± 0.1143.2 ± 9.413.52 ± 7.440%0.03 mM Apo0.48 ± 0.0537.2 ± 1.12.97 ± 2.4340 ± 20%0.3 mM Apo0.69 ± 0.06*p < 0.05 vs control.24.0 ± 4.0*p < 0.05 vs control.0.00*p < 0.05 vs control.66 ± 8%*p < 0.05 vs control.3 mM Apo0.93 ± 0.03*p < 0.05 vs control.†p < 0.05 vs 0.3 mM.12.6 ± 1.3*p < 0.05 vs control.†p < 0.05 vs 0.3 mM.0.08 ± 0.07*p < 0.05 vs control.99 ± 1%*p < 0.05 vs control.†p < 0.05 vs 0.3 mM.* p < 0.05 vs control.† p < 0.05 vs 0.3 mM. Open table in a new tab In a dose-dependent manner, Apo prevented the increased vascular permeability, pulmonary hypertension, and TX release associated with IR injury. The correlation of these effects with inhibition of O2− production from PMN leukocytes in vitro suggests that Apo protected by inhibiting leukocyte NADPH oxidase. Apo may represent a new therapeutic approach to the clinical problem of reperfusion lung injury.
Efficacy of submucosal injection of different solutions inclusive blood components on mucosa elevation for endoscopic resection (2012)

The results of the current study suggest that whole blood is more effective in generating long-lasting mucosa elevation than any other commonly used solution. Because autologous blood is readily available at almost no cost, this seems to be an optimal agent for creating the mucosa elevation needed for endoscopic resection. Further in vivo studies in humans are needed to clarify the potential role of autologous blood for long-lasting endoscopic mucosa resection or endoscopic submucosal dissection.
Antimicrobial Stewardship Programs in Community Health Systems Perceived by Physicians and Pharmacists: A Qualitative Study with Gap Analysis (2019)

Antimicrobial stewardship program (ASP) is one of the most important strategies for managing infectious disease treatment and preventing antimicrobial resistance. The successful implementation of ASP in the community health system (CHS) has been challenging. We evaluated perceptions of current ASP, potential setbacks of ASP implementation, and future demands on ASP services among physicians and pharmacists in the CHS. The qualitative research was conducted through in-depth individual interviews and focus group discussions with 11 physicians and 11 pharmacists. In addition, a quantitative gap analysis was conducted to assess the different awareness and demands on services of ASP and preferred antimicrobial-related problems (ARP). In overall, perceptions of ASP varied by profession. The identified setbacks were unorganized institutional leadership, the undefined roles of healthcare professionals, a lack of reimbursement, the hierarchical structure of the health system, and the labor-intensive working environment of pharmacy services. Although demands for ASP improvement were similar among professionals, they had different preferences in prioritizing each service item of ASP/ARP development and the profession responsible for each service. Continuous administrative and financial investments, understanding ASP contents, ASP-specific information technology, and interdisciplinary collaboration with good communication among healthcare professions are needed to continue the progression of ASP.
The use of a lidar forward-looking turbulence sensor for mixed-compression inlet unstart avoidance and gross weight reduction on a high speed civil transport (1997)

Inlet unstart causes a disturbance akin to severe turbulence for a supersonic commercial airplane. Consequently, the current goal for the frequency of unstarts is a few times per fleet lifetime. For a mixed-compression inlet, there is a tradeoff between propulsion system efficiency and unstart margin. As the unstart margin decreases, propulsion system efficiency increases, but so does the unstart rate. This paper intends to first, quantify that tradeoff for the High Speed Civil Transport (HSCT) and second, to examine the benefits of using a sensor to detect turbulence ahead of the airplane. When the presence of turbulence is known with sufficient lead time to allow the propulsion system to adjust the unstart margin, then inlet un,starts can be minimized while overall efficiency is maximized. The NASA Airborne Coherent Lidar for Advanced In-Flight Measurements program is developing a lidar system to serve as a prototype of the forward-looking sensor. This paper reports on the progress of this development program and its application to the prevention of inlet unstart in a mixed-compression supersonic inlet. Quantified benefits include significantly reduced takeoff gross weight (TOGW), which could increase payload, reduce direct operating costs, or increase range for the HSCT.