gebhart/patrepeval huggingface dataset:
Restoration of wild-type motility to flagellin-knockout Escherichia coli (2019)

Abstract Flagellin is the major constituent of the flagellar filament and faithful restoration of wild-type motility to flagellin mutants may be beneficial for studies of flagellar biology and biotechnological exploitation of the flagellar system. Therefore, we explored the restoration of motility by flagellin expressed from a variety of combinations of promoter, plasmid copy number and induction strength. Motility was only partially restored using the tightly regulated rhamnose promoter, but wild-type motility was achieved with the T5 promoter, which, although leaky, allowed titration of induction strength. Motility was little affected by plasmid copy number when dependent on inducible promoters. However, plasmid copy number was important when expression was controlled by the native E. coli flagellin promoter. Motility was poorly correlated with flagellin transcription levels, but strongly correlated with the amount of flagellin associated with the flagellar filament, suggesting that excess monomers are either not exported or not assembled into filaments. This study provides a useful reference for further studies of flagellar function and a simple blueprint for similar studies with other proteins.
Diamine Oxidase from White Pea (Lathyrus sativus) Combined with Catalase Protects the Human Intestinal Caco-2 Cell Line from Histamine Damage (2017)

Diamine oxidase (DAO) administration has been proposed to treat certain gastrointestinal dysfunctions induced by histamine, an immunomodulator, signaling, and pro-inflammatory factor. However, H<sub>2</sub>O<sub>2</sub> resulting from the oxidative deamination of histamine by DAO may be toxic. The purpose of this study was to investigate to which extent DAO from white pea (Lathyrus sativus), alone or in combination with catalase, may modulate histamine toxicity in the human intestinal Caco-2 cell line. The results show that histamine at concentrations higher than 1 mM is toxic to the Caco-2 cells, independently of the cell differentiation status, with a LC<sub>50</sub> of ≅ 10 mM following a 24-h exposure. Depending on its concentration, DAO increased histamine toxicity to a greater extent in differentiated cells compared to undifferentiated cultures. In the presence of catalase, the DAO-induced increase in histamine toxicity was completely abolished in the undifferentiated cells and only partially decreased in differentiated cells, showing differences in the sensitivity of Caco-2 cells to the products resulting from histamine degradation by DAO (H<sub>2</sub>O<sub>2</sub>, NH<sub>3</sub>, or imidazole aldehyde). It appears that treatment of food histaminosis using a combination of vegetal DAO and catalase would protect against histamine toxicity and prevent H<sub>2</sub>O<sub>2</sub>-induced damage that may occur during histamine oxidative deamination.
New Algal Species Records for Great Smoky Mountains National Park, with an Annotated Checklist of all Reported Algal Taxa for The Park (2007)

Great Smoky Mountains National Park, which straddles the Tennessee–North Carolina state-line is a biologically diverse region which has recently become the site of an All Taxon Biodiversity Inventory, an effort to chronicle species diversity by documenting all species in all phyla present within park boundaries. As a result of studies within the last two years, we have added 415 new algal taxa records. With records reported in 2004, we now have documented a total of 1000 taxa in the park. This paper summarizes all algal species found to date.
Physiology and pathophysiology of gastrin: a review. (1988)

Recent advances in understanding the physiology and pathophysiology of the gastrointestinal hormone, gastrin, are reviewed. Details of gastrin biosynthesis, secretion, and cellular actions may have broad implications for other peptide hormones. Potentially useful antigastrin drugs are described. Areas of future development are suggested.
The Contribution of Cardiovascular Magnetic Resonance Imaging to the Interrogation of Patients Considered for, Undergoing or Having Completed the Surgical Ventricular Restoration (SVR) Procedure; The Utility, Practicality and ...... (2009)

The use of Cardiovascular Magnetic Resonance (CMR) for the interrogation of the cardiovascular system is well established. As surgical techniques evolve seeking to address ischemic cardiomyopathic patients with increasing levels of cardiac functional perturbations, an imaging technique that is sufficiently comprehensive to complement these approaches is obligatory. The utility of CMR is shown for the evaluation of structure, function, morphology, viability, and intramyocardial strain by radio-frequency tissue tagging for patients with ischemic cardiomyopathy who are potential candidates for Surgical Ventricular Restoration (SVR). Using examples from the authors' experience over the last five years, representing perhaps the longest consecutive application of CMR to this complex patient group, the elements of a complete cardiac interrogation are demonstrated. Future applications for CMR aimed at the ischemic cardiomyopathic patient population are discussed, including applications to measuring diastolic function, stem cell research, and introduction of the concept of an energy model seeking to account for the mechanisms of the observed mechanical efficiency following SVR.
On the Access Complexity of PIR Schemes (2018)

Private information retrieval has been reformulated in an information-theoretic perspective in recent years. The two most important parameters considered for a PIR scheme in a distributed storage system are the storage overhead and PIR rate. The complexity of the computations done by the servers for the various tasks of the distributed storage system is an important parameter in such systems which didn't get enough attention in PIR schemes. As a consequence, we take into consideration a third parameter, the access complexity of a PIR scheme, which characterizes the total amount of data to be accessed by the servers for responding to the queries throughout a PIR scheme. We use a general covering codes approach as the main tool for improving the access complexity. With a given amount of storage overhead, the ultimate objective is to characterize the tradeoff between the rate and access complexity of a PIR scheme. This covering codes approach raises a new interesting coding problem of generalized coverings similarly to the well-known generalized Hamming weights.
Aspirin and bleeding peptic ulcers in the elderly. (1988)

A case-control study was performed to determine whether aspirin confers a similar risk of bleeding from gastric or duodenal ulcers in the elderly as non-aspirin, non-steroidal anti-inflammatory drugs. The intake of analgesics in 230 patients with bleeding ulcers aged 60 and over and in hospital and community controls matched for age and sex was examined. Those who had taken aspirin were between two and three times more likely to be admitted to hospital with bleeding ulcers. This increased risk was not accounted for by aspirin taken for indigestion or by concurrent use of non-aspirin, non-steroidal anti-inflammatory drugs. A similar effect was not seen for paracetamol. When aspirin and other non-steroidal anti-inflammatory drugs were considered together the overall risk attributed to the drugs suggested that these drugs may be responsible for over a third of admissions for bleeding peptic ulcers in the elderly.
Piezoelectric bimorph microphone built on micromachined Parylene Diaphragm (2003)

This paper describes a novel bimorph piezoelectric microphone built on a micromachined parylene diaphragm with two ZnO films of opposite c-axis orientations. Both the sensitivity and signal-to-noise ratio (SNR) of the bimorph parylene-diaphragm microphone have been demonstrated to be much higher than those of a conventional unimorph silicon-nitride-diaphragm microphone.
Factors involved in the inflammatory events of cervical ripening in humans. (2004)

There is a decrease in GR levels in human cervix at parturition. Concomitantly there is an increase of factors such as NFkappaB, PAF-R, COX-1 and COX-2, suggesting that they may participate in the sequence of events leading to the final cervical ripening.
A finite element model for insulin adsorption in ICU infusion sets (2019)

Insulin adsorption has been observed in ICU delivery lines, and is especially problematic in the delivery of insulin within the neonatal ICU. This paper presents a two state model with adsorptive loss described as a predator-prey term with parameters K<sub>i</sub> and B<sub>eq</sub>. This model is discretized to N sub volumes along the length of an infusion set. The model was found to converge to a solution for N>~100-150. The model was fit to literature data, and it was found that the total adsorptive capacity of a material (B<sub>eq</sub>, U/m<sup>2</sup>) was hyperbolically related to flow rate. If the average rate constant K<sub>i</sub> was used with the hyperbolic relationship, the model was able to describe adsorption dynamics at all 3 examined flow rates for a polyethylene line.
1-Nitro-1-(phenylthio)propene as a new nitro olefin reagent for 3-methylfuran annulation and its application to the synthesis of some furanoterpenoids (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1-Nitro-1-(phenylthio)propene as a new nitro olefin reagent for 3-methylfuran annulation and its application to the synthesis of some furanoterpenoidsMasaaki Miyashita, Toshiaki Kumazawa, and Akira YoshikoshiCite this: J. Org. Chem. 1980, 45, 15, 2945–2950Publication Date (Print):July 1, 1980Publication History Published online1 May 2002Published inissue 1 July 1980https://pubs.acs.org/doi/10.1021/jo01303a005https://doi.org/10.1021/jo01303a005research-articleACS PublicationsRequest reuse permissionsArticle Views358Altmetric-Citations58LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Diffusion filtering without parameter tuning: Models and inference tools (2010)

Relations between deterministic (e.g. variational or PDE based methods) and Bayesian inference have been known for a long time. However, a classification of deterministic approaches into those methods which can be handled within a Bayesian framework and those with no such statistical counterpart is still missing in literature. After providing such taxonomy, we present a Bayesian framework for embedding the former ones into a statistical context allowing to equip them with advantages of probabilistic estimation theory. A stochastic point of view allows us (1) to learn influence functions and derivative filter, (2) adapt diffusion and regularization approaches to changes in the image characteristics (e.g. varying noise levels), and (3) to estimate error bounds on the solution. For the latter ones we present alternative learning schemes also allowing their parameters to be related to the image statistics such that hand tuning becomes dispensable. We demonstrate that a statistical point of view on diffusion and regularization schemes leads to image denoising performances comparable with state of the art Markov random field approaches while being computationally much more effective.
Expanding the Address space through REBEKAH-IP: An Architectural View (2002)

The advent of 2.5 and 3G networks mark the beginning of the mobile Internet. The investments in these technologies will be the largest in the history of telecommunication. However, their success will largely depend on the availability of successful services and initially these will largely constitute existing services. These services all reside in the current IPv4 address space which make them inaccessible to mobile Internet terminals that will reside in the IPv6 address space. There have been many proposals in literature for translating between the two address spaces, but as we will show, they all have shortcomings that make them unsuitable for large scale deployment in the mobile Internet. In this paper, we detail the algorithms and mechanisms of our proposal for overcoming the shortcomings of the previous proposals. We also present experimental results from early experiments that justify our implementation design decisions.
Cement Salvage of Instrumentation-Associated Vertebral Fractures (2014)

Vertebral cement augmentation represents a safe and effective treatment option in patients with recurrent or progressive back pain and instrumentation-associated vertebral compression fractures.
Adaptive Selection Of Colour Transformations For Reversible Image Compression (2012)

Publication in the conference proceedings of EUSIPCO, Bucharest, Romania, 2012
Biodegradable Fiducial Markers for Bimodal Near-Infrared Fluorescence- and X-ray-Based Imaging (2022)

This study aimed to evaluate, for the first time, implantable, biodegradable fiducial markers (FMs), which were designed for bimodal, near-infrared fluorescence-based (NIRF) and X-ray-based imaging. The developed FMs had poly(l-lactide-<i>co</i>-caprolactone)-based core-shell structures made of radiopaque (core) and fluorescent (shell) composites with a poly(l-lactide-<i>co</i>-caprolactone) matrix. The approved for human use contrast agents were utilized as fillers. Indocyanine green was applied to the shell material, whereas in the core materials, iohexol and barium sulfate were compared. Moreover, the possibility of tailoring the stability of the properties of the core materials by the addition of hydroxyapatite (HAp) was examined. The performed <i>in situ</i> (porcine tissue) and <i>in vivo</i> experiment (rat model) confirmed that the developed FMs possessed pronounced contrasting properties in NIRF and X-ray imaging. The presence of HAp improved the radiopacity of FMs at the initial state. It was also proved that, in iohexol-containing FMs, the presence of HAp slightly decreased the stability of contrasting properties, while in BaSO<sub>4</sub>-containing ones, changes were less pronounced. A comprehensive material analysis explaining the differences in the stability of the contrasting properties was also presented. The tissue response around the FMs with composite cores was comparable to that of the FMs with a pristine polymeric core. The developed composite FMs did not cause serious adverse effects on the surrounding tissues even when irradiated <i>in vivo</i>. The developed FMs ensured good visibility for NIRF image-supported tumor surgery and the following X-ray image-guided radiotherapy. Moreover, this study replenishes a scanty report regarding similar biodegradable composite materials with a high potential for application.
Insulin Receptor Antibody−α-N-Acetylglucosaminidase Fusion Protein Penetrates the Primate Blood–Brain Barrier and Reduces Glycosoaminoglycans in Sanfilippo Type B Fibroblasts (2016)

Mucopolysaccharidosis Type IIIB (MPSIIIB) is caused by mutations in the gene encoding the lysosomal enzyme, α-N-acetylglucosaminidase (NAGLU). MPSIIIB presents with severe disease of the central nervous system, but intravenous NAGLU enzyme replacement therapy has not been developed because the NAGLU enzyme does not cross the blood-brain barrier (BBB). A BBB-penetrating form of the enzyme was produced by re-engineering NAGLU as an IgG-enzyme fusion protein, where the IgG domain is a monoclonal antibody (mAb) against the human insulin receptor (HIR). The HIRMAb traverses the BBB via transport on the endogenous insulin receptor and acts as a molecular Trojan horse to ferry the fused NAGLU across the BBB from blood. The NAGLU was fused to the carboxyl terminus of each heavy chain of the HIRMAb via an extended 31-amino acid linker, and the fusion protein is designated HIRMAb-LL-NAGLU. The fusion protein retains high affinity binding to the HIR, and on a molar basis has an enzyme activity equal to that of recombinant human NAGLU. Treatment of MPSIIIB fibroblasts with the fusion protein normalizes intracellular NAGLU enzyme activity and reduces sulfate incorporation into intracellular glycosoaminoglycan. The fusion protein is targeted to the lysosomal compartment of the cells as shown by confocal microscopy. The fusion protein was radiolabeled with the [(125)I]-Bolton-Hunter reagent and injected intravenously in the adult Rhesus monkey. The fusion protein was rapidly cleared from plasma by all major peripheral organs. The high brain uptake of the fusion protein, 1% injected dose/brain, enables normalization of brain NAGLU enzyme activity with a therapeutic dose of 1 mg/kg. The HIRMAb-LL-NAGLU fusion protein is a new treatment of the brain in MPSIIIB, which can be administered by noninvasive intravenous infusion.
DNA Sequence Variants in Epithelium-Specific ETS-2 and ETS-3 Are Not Associated with Asthma (2002)

Epithelium-specific ETS-2 and ETS-3 are transcription factors that have been proposed as asthma candidate genes. To investigate the association of sequence variants in these genes with asthma, we conducted a case-control association analysis in a sample of 311 white subjects with asthma and 177 white subjects without asthma. Common polymorphisms in these genes were detected by sequencing DNA from 32 cell lines obtained from Coriel (Camden, NJ). Seven noncoding or synonymous single-nucleotide polymorphisms were detected: three in epithelium-specific ETS-2 and four in epithelium-specific ETS-3. Subjects were genotyped at all loci by mass spectroscopy. To ensure the suitability of our control subjects, we also genotyped subjects at 49 unlinked polymorphisms evenly distributed throughout the autosomes and found no evidence of population stratification. Logistic regression adjusted for age and sex suggested a weak association of one epithelium-specific ETS-2 polymorphism with asthma diagnosis (odds ratio = 1.89, 95% confidence interval = 1.13-3.18, p = 0.02). Total serum immunoglobulin E and FEV1 predicted levels were not associated with any of the polymorphisms. Extended haplotyping indicated linkage disequilibrium in these genes; however, no association or epistatic interaction was found. This study suggests that epithelium-specific ETS-2 and ETS-3 genes are unlikely to contain polymorphic loci that have a major impact on asthma susceptibility in our population.
Evaluation of User Perceived Performance in Sparce Infrastructure Wireless Systems (2004)

This work tries to evaluate the user perceived performance in a wireless system based on sparse infrastructure (islands of coverage).We investigate a mobile user in a Manhattan environment and the considered service is web browsing. Data caching in the use terminal is employed in order to hide the discontinuities of coverage. We show that for this particular application, the user's behavior of revisiting pages previously accessed makes caching a way to increase the user perceived coverage. Our results show that at low levels of effective area coverage caching brings a large improvement, while the gain is not significant at high levels. If the sparse coverage network is overlaid over a cellular system the obtained results show potential for significant cost savings.
Role of the hypothalamus-pituitary-adrenal axis in the regulation of TNF production in mice. Effect of stress and inhibition of endogenous glucocorticoids (1995)

Glucocorticoids (GC) are known inhibitors of TNF production and are increased by endotoxin (LPS) through a stimulation of the hypothalamus-pituitary-adrenal axis (HPAA), suggesting a feedback mechanism. We tried different approaches to study the role of the HPAA and endogenous GC in the regulation of TNF production. Cyanoketone, a GC synthesis inhibitor, inhibited corticosterone (CS) induction by LPS and increased LPS-induced serum TNF levels. Similar results were obtained by pretreating mice with anticorticotropin-releasing hormone Abs. Administration of adrenocorticotropic hormone increased blood CS and inhibited LPS-induced serum TNF. TNF production by mouse blood stimulated in vitro with LPS was inhibited by addition of CS. Blood from stressed or adrenocorticotropic-releasing hormone-treated mice (in which CS levels are elevated) stimulated ex vivo with LPS produced significantly less TNF than blood from control mice. Normal TNF production was restored by the addition of the GC receptor antagonist RU 38486, indicating a role for the elevated endogenous CS levels in the inhibition of TNF production. These data indicate that the HPAA is a major regulator of TNF production.
Comparison of Lab Versus Engine Tests In the Development of a Highly Efficient Ammonia Formation Catalyst for a Passive SCR System (2015)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;Commercial three way catalysts have limited capacity towards reducing NO&lt;sub&gt;x&lt;/sub&gt; in the presence of excessive oxygen. This prevents lean-burn combustion concepts from meeting legislative emission standards. A solution towards decreasing NO&lt;sub&gt;x&lt;/sub&gt; emissions in the presence of excess air is the use of a passive-SCR system. Under rich conditions ammonia is formed over an ammonia formation catalyst, the ammonia is stored in the SCR and in its turn reacts with the NO&lt;sub&gt;x&lt;/sub&gt; under lean engine conditions.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;Here up-scaled Pt/Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt; and Pd/Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt; catalysts as well as a commercially Pd-Rh based three-way catalyst (TWC) are evaluated using both engine and further lab-scale tests.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;The purpose of these tests is to compare the ammonia production for the various catalysts under various lambda values and temperatures by means of engine and lab scale tests. The Pd/Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt; showed little sensitivity to temperature both under engine and lab scale experiments. The Pt/Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt; was affected to a large extend by temperature for both test methods. The TWC showed stable production during the engine measurements while under lab tests an increased temperature resulted in a lower ammonia yield.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;Differences between the engine and lab scale tests are mainly due to catalyst temperatures, space velocity, CO poisoning and uncertainties in the composition of the engine's exhaust gas. Both Pt/Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt; and Pd/Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt; form ammonia although the former generate higher amounts at high temperature but are believed to suffer from CO poisoning at low temperatures.&lt;/div&gt;&lt;/div&gt;