gebhart/patrepeval huggingface dataset:
Identification of the active site serine in pancreatic cholesterol esterase by chemical modification and site-specific mutagenesis. (1990)

Chemical modification and site-specific mutagenesis approaches were used in this study to identify the active site serine residue of pancreatic cholesterol esterase. In the first approach, purified porcine pancreatic cholesterol esterase was covalently modified by incubation with [3H]diisopropylfluorophosphate (DFP). The radiolabeled cholesterol esterase was digested with CNBr, and the peptides were separated by high performance liquid chromatography. A single 3H-containing peptide was obtained for sequence determination. The results revealed the binding of DFP to a serine residue within the serine esterase homologous domain of the protein. Furthermore, the DFP-labeled serine was shown to correspond to serine residue 194 of rat cholesterol esterase (Kissel, J. A., Fontaine, R. N., Turck, C. W., Brockman, H. L., and Hui, D. Y. (1989) Biochim. Biophys. Acta 1006, 227-236). The codon for serine 194 in rat cholesterol esterase cDNA was then mutagenized to ACT or GCT to yield mutagenized cholesterol esterase with either threonine or alanine, instead of serine, at position 194. Expression of the mutagenized cDNA in COS-1 cells demonstrated that substitution of serine 194 with threonine or alanine abolished enzyme activity in hydrolyzing the water-soluble substrate, p-nitrophenyl butyrate, and the lipid substrates cholesteryl [14C]oleate and [14C] lysophosphatidylcholine. These studies definitively identified serine 194 in the catalytic site of pancreatic cholesterol esterase.
An(III) and Ln(III) complexation with TPAEN: selectivity quantification for an americium(III) separation process (2016)

International audience
ChemInform Abstract: Buchner Reaction (2011)

Abstract Review: 41 refs.
Pyrene-substituted ethenes: aggregation-enhanced excimer emission and highly efficient electroluminescence (2011)

Pyrene-substituted ethenes, 1,2,2-tripheny-1-pyrenylethene (TPPyE) and 1,2-diphenyl-1,2-dipyrenylethene (DPDPyE), are synthesized and characterized. Whereas they are weakly emissive in solution they become strong emitters when aggregated in the condensed phase. In contrast to the general observation that excimer formation quenches the light emission of fluorophores, TPPyE and DPDPyE exhibit efficient excimer emissions in the solid state with high fluorescence quantum yields up to 100%. The π–π intermolecular interactions between the pyrene rings, coupled with multiple C–H⋯π hydrogen bonds, efficiently restrict intramolecular rotations, which block the nonradiative energy decay channel, and hence make the dye molecules highly emissive in the solid state. Non-doped organic light-emitting diodes using TPPyE and DPDPyE as emitters are fabricated, which give green light at low turn-on voltages (down to 3.2 V) with maximum luminance and power, current, and external quantum efficiencies of 49830 cd m−2, 9.2 lm W−1, 10.2 cd A−1 and 3.3%, respectively.
New developments in photo-aligning and photo-patterning technologies: physics and applications (2016)

The effect of liquid crystal (LC) photoaligning is a direct consequence of the appearance of the photo-induced optical anisotropy and dichroism absorption in thin amorphous films, formed by molecular units with anisotropic absorption properties. As polyimide materials possess high temperature stability and are commonly used in the liquid crystal display (LCD) industry, which is highly desirable to modify them for the photo-alignment applications. The value of the pretilt angle of an LC cell on the substrate is one of the important characteristics for photo-alignment. In most of the electro-optical modes the pretilt angle should be sufficiently high to enable a quality performance of LCD. A new generation of optical security elements based on photo-patterning technology enables the fabrication of optical security devices. The photo-patterned security mark can be applied directly to documents via printing or coating methods as well as indirectly by hot-stamping processes.
Wilms tumor, pleuropulmonary blastoma, and DICER1: case report and literature review (2018)

This case highlights that in case of simultaneous discovery of a renal tumor and a pulmonary lesion in a child, the DICER 1 mutations should be looked for as these could help adapt management and schedule the surgical procedures.
A 2.2μm stacked back side illuminated voltage domain global shutter CMOS image sensor (2019)

This paper presents a 2.2μm pixel pitch back side illuminated (BSI) Voltage Domain Global Shutter (VDGS) image sensor with Stacked Pixel Level Connection (SPLC) and full backside Deep Trench Isolation (DTI). With these cutting edge technologies, Full Well Capacity (FWC) more than 12,000 electrons and parasitic light sensitivity (PLS) larger than 100 dB are reached. A 38% Quantum Efficiency (QE) and 60% of Modulation Transfer Function (MTF) at 940nm, half Nyquist frequency (Ny/2) is demonstrated.
Chromosome Mapping and Expression of HumanTip49Family Genes (2000)

TBP-interacting protein 49 (TIP49) was originally identified as a TBP-binding protein, and two related proteins are encoded by individual genes, tip49a and b. Although the function of this gene family has not been elucidated, they are supposed to play a critical role in nuclear events because they interact with various kinds of nuclear factors and have DNA helicase activities. At least, TIP49a has been suggested to act as an autoantigen in some patients with autoimmune diseases. In this study, we investigated the chromosome positions of this family of genes. Human tip49a and tip49b genes were mapped on 3q21 and 19q13.2, respectively. Consistent with the notion that tip49 family genes are essential for cell growth, Northern blot analysis demonstrated that both genes are expressed ubiquitously in human tissues. It is worthy of notice that the testes contained large amounts of the both transcripts. These results are consistent with our previous results from tissue distribution analysis for of TIP49 proteins.
Monofunctionalized Tetraazacrowns for Use in Bioconjugation and Catalysis (2005)

Alkylation of 1,4,7,11‐tetraazacyclododecane (cyclen) with 4‐nitrobenzyl bromide, p‐chloromethylbenzyl alcohol, 4‐iodobenzyl bromide, or 4‐vinylbenzyl bromide in acetonitrile gave N‐monoalkylated cyclen ligands 1–4 in 38–60% yield. Compounds 3 and 4 reacted with cobalt chloride hexahydrate in methanol, followed by air oxidation and addition of HCl, gave cobalt complexes dichloro{N‐(4‐iodobenzyl)‐1,4,7,10‐tetraazacyclotetradecane}cobalt(III) chloride (5) and dichloro{N‐(4‐vinylbenzyl)‐1,4,7,10‐tetraazacyclotetradecane}cobalt(III) chloride (6) in 34–55% yield. Compounds 1–6 were characterized by 1H NMR, ESI‐MS, UV‐visible, and IR spectroscopies.
Sidewalk Construction 116th Street and US 31 (2016)

&lt;p&gt;[[Video(http://www.youtube.com/watch?v=BIHsigkBih0)]]&lt;/p&gt; &lt;p&gt;The construction of the bridge shown in this video is part of a $350,000,000 project that upgrades and grade-separates 13 miles of US 31 between I-465 and Indiana State Road 38. The upgraded 13-mile stretch features 11 new interchanges and eliminates 32 traffic signals. The overall project was substantially completed in December 2015. This video shows time-lapse documentation of the construction of a concrete sidewalk at the intersection of US 31 and 116th Street in Carmel, Indiana.&lt;/p&gt; &lt;p&gt;The project is located at &lt;a href="https://www.google.com/maps/@39.9561826,-86.1573638,18.78z"&gt;39.9561826,-86.1573638&lt;/a&gt;.&lt;/p&gt;
Specific Genomic Alterations in High-Grade Pulmonary Neuroendocrine Tumours with Carcinoid Morphology (2020)

These data confirm the importance of chromatin remodelling genes in pulmonary carcinoids and highlight the potential role of TP53 and RB1 to drive the transformation in more aggressive high-grade tumours.
Isolation and characterization of Human Mesenchymal Stromal Cells Derived from Placental Decidua Basalis; Umbilical cord Wharton’s Jelly and Amniotic Membrane (1969)

The fetal derives stromal cells; especially the early passages of WJ-MSCs are available supplies for large scale production of MSC for using in clinical studies or research projects.
Application for White Spot Syndrome Virus (WSSV) Monitoring using Edge Machine Learning (2023)

The aquaculture industry, strongly reliant on shrimp exports, faces challenges due to viral infections like the White Spot Syndrome Virus (WSSV) that severely impact output yields. In this context, computer vision can play a significant role in identifying features not immediately evident to skilled or untrained eyes, potentially reducing the time required to report WSSV infections. In this study, the challenge of limited data for WSSV recognition was addressed. A mobile application dedicated to data collection and monitoring was developed to facilitate the creation of an image dataset to train a WSSV recognition model and improve country-wide disease surveillance. The study also includes a thorough analysis of WSSV recognition to address the challenge of imbalanced learning and on-device inference. The models explored, MobileNetV3-Small and EfficientNetV2-B0, gained an F1-Score of 0.72 and 0.99 respectively. The saliency heatmaps of both models were also observed to uncover the "black-box" nature of these models and to gain insight as to what features in the images are most important in making a prediction. These results highlight the effectiveness and limitations of using models designed for resource-constrained devices and balancing their performance in accurately recognizing WSSV, providing valuable information and direction in the use of computer vision in this domain.
Organizational commitment, job satisfaction and intention to leave: A comparative analysis between part-time and full-time faculties (2008)

The importance of part-time work has been growing in recent years, due to its significant increase in today's societies, and higher education institutions have not been alien to this trend. The present research tries to study the relationship between organizational commitment and job satisfaction with the intention to leave the institution, comparing part-time and full-time faculty. An empirical research, grounded in the model proposed by Currivan (1999), has been undertaken, with a sample of faculty of ETSEIAT, a college of the Technical University of Catalonia. Results show the existence of the relationships with organizational commitment, job satisfaction and intention to leave predicted in the literature, and significant differences in job satisfaction and organizational commitment between part-time and full-time faculty. The paper ends with some proposals of further research.
A study on the recognition and requirement of dental hygienists for enforcing specialty (2010)

Objectives : This study was to seek the ways for strengthening the higher curricula for the bachelor's degree of the dental hygiene. For this two things are investigated with some students of the dental hygiene departments of colleges and workers on the part; one is what the recognition about the curricula is, and the other what are the demanding degrees through the curricula. Methods : The study has been performed with 298 people who work for dental hygiene of dental clinics and students of the departments in B region by using structured and self-administered questionnaire from March to May, 2008, and were analyzed by using SPSS 14.0 program. Results : The rate to recognize the higher curricula for the bachelor degree is 88.9%. And 70.4% among them says the curricula is 'absolutely necessary' or 'necessary'. The ways to recognize the curricula are through 'professor or friends'(53.4%), through 'newspapers of broad-casting reports'(20.5%), and through 'internet'(14.4%). They want various teaching ways including the project-centered education(40.4%), lecture-like education(25.0%), field-experience within the workplace(14.4%), lectures in the workplace(10.6%), and discussion(9.6%). Those who works in the dental clinics want night classes(49.5%, 96 respondents), internet classes(16.5%, 32 respondents), and weekend classes(14.4%, 28 respondents). They like to learn majors more deeply(30.9%), important techniques and theories about their job(26.8%), and the new skills on their work(19.8%). Conclusions : persons who are workers for dental hygiene of dental clinics and students of the departments have a requirement of dental hygienists for enforcing specialty. Therefor improve to develop education program and system about development of advance course for hygienists.
Monolithic optofluidic mode coupler for broadband thermo- and piezo-optical characterization of liquids (2017)

We present a monolithic fiber device that enables investigation of the thermo- and piezo-optical properties of liquids using straightforward broadband transmission measurements. The device is a directional mode coupler consisting of a multi-mode liquid core and a single-mode glass core with pronounced coupling resonances whose wavelength strongly depend on the operation temperature. We demonstrated the functionality and flexibility of our device for carbon disulfide, extending the current knowledge of the thermo-optic coefficient by 200 nm at 20 °C and uniquely for high temperatures. Moreover, our device allows measuring the piezo-optic coefficient of carbon disulfide, confirming results first obtained by Röntgen in 1891. Finally, we applied our approach to obtain the dispersion of the thermo-optic coefficients of benzene and tetrachloroethylene between 450 and 800 nm, whereas no data was available for the latter so far.
Characterisation of adipocyte‐derived extracellular vesicles released pre‐ and post‐adipogenesis (2015)

Extracellular vesicles (EVs) are submicron vesicles released from many cell types, including adipocytes. EVs are implicated in the pathogenesis of obesity-driven cardiovascular disease, although the characteristics of adipocyte-derived EVs are not well described. We sought to define the characteristics of adipocyte-derived EVs before and after adipogenesis, hypothesising that adipogenesis would affect EV structure, molecular composition and function. Using 3T3-L1 cells, EVs were harvested at day 0 and day 15 of differentiation. EV and cell preparations were visualised by electron microscopy and EVs quantified by nanoparticle tracking analysis (NTA). EVs were then assessed for annexin V positivity using flow cytometry; lipid and phospholipid composition using 2D thin layer chromatography and gas chromatography; and vesicular protein content by an immuno-phenotyping assay. Pre-adipogenic cells are connected via a network of protrusions and EVs at both time points display classic EV morphology. EV concentration is elevated prior to adipogenesis, particularly in exosomes and small microvesicles. Parent cells contain higher proportions of phosphatidylserine (PS) and show higher annexin V binding. Both cells and EVs contain an increased proportion of arachidonic acid at day 0. PREF-1 was increased at day 0 whilst adiponectin was higher at day 15 indicating EV protein content reflects the stage of adipogenesis of the cell. Our data suggest that EV production is higher in cells before adipogenesis, particularly in vesicles <300 nm. Cells at this time point possess a greater proportion of PS (required for EV generation) whilst corresponding EVs are enriched in signalling fatty acids, such as arachidonic acid, and markers of adipogenesis, such as PREF-1 and PPARγ.
Analysis of Repeated Measurements with Ante‐Dependence Covariance Models (1997)

Abstract This paper considers the use of ante‐dependence models in problems with repeated measures through time. These are conditional regression models which reflect the dependence of a measure on some of the previous observations from the same subject. We present maximum likelihood estimators of the covariance matrix and procedures for selecting the order of ante‐degendence based on penalized like‐lihoods. Extensions to missing data situations are discussed. We propose Wald‐type test statistics and apply them in two situations common in experiments with repeated measures: one with pre‐study observations and another one with small sample size relative to the number of time periods. In these examples, tests assuming ante‐dependence find effects which are not detected using competing procedures.
Quasi-modular Pseudo-Complemented Semi-lattices (2016)

In this paper we dene and introduce a new class of pseudocomplemented semilattices ; the class of quasi-modular pseudocomplemented semilattices; which generalizes modular pseudocomplemented semilattices.We give a characterization and a construction of such class, using a triple containing a Boolean algebra, a semilattice with a greatest element and a structure map. We describe homomorphisms, subalgebras and congruence relations of the quasi-modular pseudo-complemented semi-lattices by means of triples.
Prucalopride exerts neuroprotection in human enteric neurons (2016)

Serotonin (5-hydroxytryptamine, 5-HT) and its transporters and receptors are involved in a wide array of digestive functions. In particular, 5-HT4 receptors are known to mediate intestinal peristalsis and recent data in experimental animals have shown their role in neuronal maintenance and neurogenesis. This study has been designed to test whether prucalopride, a well-known full 5-HT4 agonist, exerts protective effects on neurons, including enteric neurons, exposed to oxidative stress challenge. Sulforhodamine B assay was used to determine the survival of SH-SY5Y cells, human enteric neurospheres, and ex vivo submucosal neurons following H2O2 exposure in the presence or absence of prucalopride (1 nM). Specificity of 5-HT4-mediated neuroprotection was established by experiments performed in the presence of GR113808, a 5-HT4 antagonist. Prucalopride exhibited a significant neuroprotective effect. SH-SY5Y cells pretreated with prucalopride were protected from the injury elicited by H2O2 as shown by increased survival (73.5 ± 0.1% of neuronal survival vs. 33.3 ± 0.1%, respectively; P < 0.0001) and a significant reduction of proapoptotic caspase-3 and caspase-9 activation in all neurons tested. The protective effect of prucalopride was reversed by the specific 5-HT4 antagonist GR113808. Prucalopride promotes a significant neuroprotection against oxidative-mediated proapoptotic mechanisms. Our data pave the way for novel therapeutic implications of full 5-HT4 agonists in gut dysmotility characterized by neuronal degeneration, which go beyond the well-known enterokinetic effect.
Multiphoton-multiplasmon Excitation Of C/sub60/ (1997)

Time-of-flight mass spectrometry is used to study the interaction of free C60 molecules with ultrashort high power Ti:sapphire laser pulses. Ionization and fragmentation of C60 by ultrashort high-intensity laser pulses occur predominantly via plasmon excitation. Single and double excitation of collective charge oscillation is observed by remarkably large number of photons. The difference between apparent threshold energies for each ionization or fragmentation and the much lower ionization potentials is a natural consequence of primary excitation process.
Multi-centre randomised controlled trial comparing arthroscopic hip surgery to physiotherapist-led care for femoroacetabular impingement (FAI) syndrome on hip cartilage metabolism: the Australian FASHIoN trial (2021)

Australia New Zealand Clinical Trials Registry reference: ACTRN12615001177549 . Trial registered 2/11/2015.
Lipid specificity and location of the sterol carrier protein‐2 fatty acid‐binding site: A fluorescence displacement and energy transfer study (1997)

Although it was recently recognized that sterol carrier protein-2 (SCP-2) interacts with fatty acids, little is known regarding the specificity of SCP-2 for long-chain fatty acids or branched-chain fatty-acid-like molecules. Likewise the location of the fatty-acid binding site within SCP-2 is unresolved. A fluorescent cis-parinaric acid displacement assay was used to show that SCP-2 optimally interacted with 14-22 carbon chain lipidic molecules: polyunsaturated fatty acids > monounsaturated, saturated > branched-chain isoprenoids > branched-chain phytol-derived fatty acids. In contrast, the other major fatty-acid binding protein in liver, fatty-acid binding protein (L-FABP), displayed a much narrower carbon chain preference in general: polyunsaturated fatty acids > branched-chain phytol-derived fatty acids > 14- and 16-carbon saturated > branched-chain isoprenoids. However, both SCP-2 and L-FABP displayed a very similar unsaturated fatty-acid specificity profile. The presence and location of the SCP-2 lipid binding site were investigated by fluorescence energy transfer. The distance between the SCP-2 Trp50 and bound cis-parinaric acid was determined to be 40 A. Thus, the SCP-2 fatty-acid binding site appeared to be located on the opposite side of the SCP-2 Trp50. These findings not only contribute to our understanding of the SCP-2 ligand binding site but also provide evidence suggesting a potential role for SCP-2 and/or L-FABP in metabolism of branched-chain fatty acids and isoprenoids.
A simple and efficient approach to capturing bonding effect in naturally microstructured sands by discrete element method (2006)

Abstract A discrete element modelling of naturally microstructured sands is very important to geomechanics. This paper presents a simple discrete element model for naturally microstructured sands with the aim to efficiently capture the effect of cementation between particles (bonds). First, a simple bond contact model was proposed by introducing a rigid‐plastic bond element into the conventional contact model for dry granular material. Second, efficient numerical techniques were investigated to implement this contact model into the distinct element method (DEM). Then, a two‐dimensional DEM code was developed to simulate a series of isotropic compression tests on the materials of different densities and bonding strengths. Finally, the DEM results were examined in comparison with the experimental data on artificially bonded sands obtained by Rotta et al . ( Géotechnique 2003; 53 (5):493–502). In addition, we discussed the yielding mechanism, the Coop and Willson criteria on weak/strong bonding ( J . Geotech . Eng . (ASCE) 2003; 129 (11):1010–1019) and the strong bonding phenomenon observed by Rotta et al. based on the DEM data. The study shows that the DEM model is able to capture the main features of naturally microstructured sands, such as variations of yielding and bulk modulus against bonding strength or material density. In addition, it is shown that the gross yielding (the yielding defined in terms of strains) is largely related to bond breakage; Coop and Willson criteria are generally reasonable; and the strong bonding in the experimental data obtained by Rotta et al . comes from that their bonded materials start at different points on the same compression line. Copyright © 2006 John Wiley &amp; Sons, Ltd.