gebhart/patrepeval huggingface dataset:
Efficient Selection of WT1-Specific Donor Lymphocytes by Streptamer Technology. (2009)

Abstract Abstract 3311 Poster Board III-199 BACKGROUND After allogeneic hematopoietic stem cell transplantation, donor lymphocyte infusions (DLIs) have the potential to generate a desirable graft-versus-leukemia (GVL) effect, but bear the risk of eliciting a noxious graft-versus-host disease (GVHD). To minimize the risk of a GVHD and to improve the GVL effect, a positive selection of leukemia (antigen)-specific T cells would be highly desirable. In this study we focused on the leukemia-antigen Wilms Tumor gene 1 (WT1). In patients with myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), a good correlation of WT1 expression and the number of leukemia blasts could be demonstrated. Several groups described immunogenic T cell epitopes derived from the WT1 protein. MATERIAL AND METHODS Here, we used the technology of streptamers available on a GMP level to detect the frequency of HLA-A2 restricted CD8+ T cells in the naïve peripheral blood (PB) from both healthy donors (HDs) and AML patients. Such WT1-specific CD8+ T cells were further characterized for the expression of CD27, CD28, CD45RA, CCR7 and CD107a. In the next step, WT1-specific cells were positive selected by magnetic cell separation (MACS™) columns after labeling with streptamers or tetramers and thereafter immunophenotyped. Moreover, mixed lymphocyte peptide cultures (MLPCs) were performed to enrich WT1-specific T cells derived from the PB of HDs. At last, WT1 specific T cells were evaluated in CFSE-based proliferation and cytotoxicity assays. Streptamer selected WT1-specific CD8+ T cells were compared with tetramer selected WT1-specific CD8+ T cells. RESULTS 21 of 40 HDs showed naïve WT1 specific T cell frequencies of 0.5 to 2.0% of all CD8+ T cells. In two of ten AML patients, also 0.4 to 3.6% of WT1-specific T cells could be detected. Through streptamer based MACS™, a purity of more than 90% of all CD8+ T cells could be achieved for WT1-specific CD8+ T cells. These cells revealed to be CD8+WT1 _Streptamer+CD28+ CD45RA+CD69-CD107a+CCR7-CD137- effector T cells in flow cytometry. Proliferation assays proved that the streptamer technology does not alter the proliferative potential of the WT1-specific CD8+ T cells. In cytotoxicity assays, WT1-specific CD8+ T cells after streptamer selection were able to lyse HLA-A2+WT1+ cells at an effector/target ratio of 20:1. No significant difference between streptamer and tetramer selection could be found for the phenotype, as well as for the proliferative and cytotoxic potential of the cells. After a maximum of three rounds of MLPC, only a frequency of 2-5% could be achieved, thus demonstrating the power of the streptamer technology. CONCLUSION In summary, the streptamer technology allows to select a highly purified fraction of WT1-specific effector T cells with proliferative and cytotoxic properties. In analogy to DLIs specific for viral antigens, production of leukemia specific DLIs is feasible on a GMP level. Further leukemia antigens are currently evaluated by our group. Disclosures No relevant conflicts of interest to declare.
Isolation and characterization of diesel oil degrading indigenous microrganisms in Kwazulu-Natal, South Africa (2008)

&nbsp; Uncontrolled releases of petroleum compounds that are carcinogenic, mutagenic and are potent immunotoxicants into soil and groundwater poses a serious threat to human and animal health. Biodegradation of hydrocarbon-contaminated soils has been established as an efficient, economic, versatile and environmentally sound treatment. Ten indigenous microorganisms were isolated from contaminated soils using the enrichment technique. Five isolates with the highest degradation potentials under standard degradation conditions were identified asAcinetobacter calcoaceticus (LT1&nbsp;and ETS2),&nbsp;Acinetobacter&nbsp;sp.&nbsp;(LT1A),Citrobacter freundii&nbsp;(MRC3) and&nbsp;Bacillus pumilus&nbsp;(JLB).&nbsp;B. pumilus&nbsp;achieved 86.94% of diesel degradation in 2 weeks. Additional degradation assay was carried out in liquid media using 3 local commercial fertilizers as nutrient supplements in comparison with the Bushnell-Haas (BH) media. The results show that the addition of fertilizer F1 stimulated diesel degradation by all isolates especially&nbsp;B. pumilus&nbsp;while the addition of fertilizer F3 seemed to strongly inhibit the bacterial ability of diesel degradation. The inoculation with the consortia did not show a higher degradation potential than the individual isolate. The results strongly indicate that environmental conditions of the contaminated sites play a crucial role in the degradation even though additional diesel-degrader has been introduced into the contaminated site. &nbsp; Key words:&nbsp;Diesel bioremediation,&nbsp;Bacillus&nbsp;sp,&nbsp;Acinetobacter calcoaceticussp.,&nbsp;Citrobacter freundii.
Single-cell spatial landscapes of the lung tumour immune microenvironment (2022)

<strong>We updated our data set, please use the new version (md5:e33079d5299cdc17f37b9f5e4c08be12).</strong> All data supporting the findings of the publication <strong>"Single-cell spatial landscapes of the lung tumour immune microenvironment"</strong>, including masks of high-dimension tif images, single-cell segmentation, single-cell cell types, and patient data. The code used to produce the results of this study is available at https://github.com/walsh-quail-labs/IMC-Lung. Channel index names: Channel Name Channel Index CD117 1 CD11c 2 CD14 3 CD163 4 CD16 5 CD20 6 CD31 7 CD3 8 CD4 9 CD68 10 CD8a 11 CD94 12 DNA1 13 FoxP3 14 HLA-DR 15 Histone H3 16 MPO 17 Pancytokeratin 18 TTF1 19
Understanding multi-scale battery degradation with a macro-to-nano zoom through its hierarchy (2021)

Lithium-ion batteries (LIBs) feature structural and chemical complexities across a broad range of length scales.
Active arc suppression device based on voltage‐source convertor with consideration of line impedance in distribution networks (2021)

Abstract In the non‐effectively grounding distribution system, residual current under single‐line‐to‐ground (SLG) fault threatens the safety of human being and power supply equipment. Active arc suppression device has been proved to be effective for SLG fault arc suppression when the line impedance is ignored. However, in practice, line impedance varies with the fault location and the load current flowing through the impedance brings about additional voltage drop, which increases the fault current and is not dealt with by the conventional methods. To achieve accurate SLG fault arc suppression with the existence of line impedance, the neutral‐to‐ground voltage reference for full ground‐fault current compensation is firstly derived and a detection method is then proposed. The pre‐fault and post‐fault line currents are used to eliminate the influence of load current on the line impedance voltage drop. A dual‐loop voltage and current controller is then designed. The prototype of active arc suppression device was developed. The results of simulation and prototype experiment validate the effectiveness of the proposed method.
ChemInform Abstract: CYCLIC PEROXIDES. 97. SINGLET OXYGENATION OF CIS,CIS‐1,5‐CYCLOOCTADIENE: A CONVENIENT SYNTHETIC ENTRY INTO 5,8‐DIFUNCTIONALIZED OXYGEN DERIVATIVES OF 1,3‐CYCLOOCTADIENE (1980)

Abstract Die sensibilisierte Photooxidation von cis, cis‐l ,5‐Cyclooctadien (I) liefert über die Stufe des Hydroperoxids (II) ein Bis‐hydroperoxid (III), das zu (IV) reduziert und dann katalytisch zu (V) hydriert werden kann.
Coarsening in Nonlocal Interfacial Systems (2008)

We consider coarsening in interfacial systems driven by nonlocal energies. Of particular interest are the nonlocal Cahn–Hilliard equation and models of biological aggregation. The energies considered cause the system to separate into phases. The pattern of interfaces evolves under nonlocal surface-tension-type effects. The typical length scales grow and the pattern coarsens. We prove a rigorous upper bound on the coarsening rate. The proof uses the energy-based approach to estimates on rate of coarsening introduced by Kohn and Otto [Comm. Math. Phys., 229 (2002), pp. 375–395]. To show the required estimates on the flatness of the energy landscape we develop a geometric approach which is applicable to a wider class of problems, which includes ones based on local, gradient-type energies.
On the rheology of packed polymer powders (1980)

Abstract The rheology of polystyrene powder beds was examined by two kinds of experiments. From stress relaxation measurements during powder compaction, it was found that relaxation at the shorter times could be attributed to sliding between particles. This is followed by a relaxation attributed to the deformation of the polystyrene particles themselves. From dynamic mechanical measurements, an increase was found in the value of vibrational absorption coefficient ( Q −1 value) at a temperature below that of the main relaxation dispersion of bulk polystyrene, attributed to the slipping around tight particle junctions. These phenomena appear characteristic of powder‐like materials.
BPMN+I to support decision making in innovation management for automated production systems including technological, multi team and organizational aspects (2020)

A joined interdisciplinary approach from systems engineering, organizational sociology and psychology is introduced using an enriched Business Process Model and Notation (BPMN+I) based modeling approach to support decision making on a management level for both mid-term decisions such as in-/outsourcing and short-term decisions such as fixing a weakness on site during start-up of a plant abroad or involving the design offices. This approach focusses on the actual collaboration between interdisciplinary teams within an organizational context by enriching BPMN with checklists applicable to all interfaces along the projects’ workflow. Our contribution aims at supporting innovation management for automated Production Systems which depends on successful interdisciplinary collaboration.
Preparation of immunochromatographic strips for rapid detection of early secreted protein ESAT-6 and culture filtrate protein CFP-10 from Mycobacterium tuberculosis (2017)

The early secreted protein early secretory antigenic target 6(ESAT-6) and the culture filtrate protein 10 (CFP-10) are 2 antigens that are specific to Mycobacterium tuberculosis. These 2 antigens are good targets for tuberculosis (TB) detection.To rapidly diagnose TB across a variety of samples, we developed colloidal gold immunochromatographic strips (ICSs) based on ESAT-6 and CFP-10.The strips were evaluated using 233 samples, including sputum, plasma, and pleural effusion samples.The positive detection rates for ICSs for ESAT-6 and CFP-10 in sputum (culture-positive for M tuberculosis) were 100% and 91.2%, respectively. The positive detection rates for ICSs for ESAT-6 and CFP-10 in plasma were 34.1% and 29.4%, respectively. The positive detection rates for ICSs for ESAT-6 and CFP-10 in pleural effusion were 64.7% and 55.9%, respectively. Experimental analysis of culture supernatant showing that the ICS developed for ESAT-6 had a sensitivity of 100% and a specificity of 91.2%. While the ICS developed for CFP-10 had a sensitivity of 91.2% and a specificity of 88.2%.The validity of the test is limited by source of sample. The technique is sensitive and specific for samples in sputum and culture media but not for plasma or pleural effusion samples. Detection of M tuberculosis using ICSs is rapid, simple, and relatively effective; thus, ICSs are a potential screening tool for TB.
Resonant frequency calculation for circular microstrip antennas using artificial neural networks (1998)

A new method based on artificial neural networks for calculating the resonant frequency of circular microstrip patch antennas is presented. This neural method is simple and is useful for the computer-aided design (CAD) of microstrip antennas. The theoretical resonant frequency results obtained by using the method are in very good agreement with the experimental results reported elsewhere. © 1998 John Wiley & Sons, Inc. Int J RF and Microwave CAE 8: 270–277, 1998.
Approaches to sample size estimation in the design of clinical trials—a review (1984)

Abstract Over the last decade, considerable interest has focused on sample size estimation in the design of clinical trials. The resulting literature is scattered over many textbooks and journals. This paper presents these methods in a single review and comments on their application in practice.
Palladium-Catalyzed Enantioselective α-Arylation and α-Vinylation of Oxindoles Facilitated by an Axially Chiral P-Stereogenic Ligand (2009)

The enantioselective alpha-arylation and alpha-vinylation of oxindoles catalyzed by Pd and a biarylmonophosphine ligand with both axial and phosphorus-based chirogenicity is reported. The resultant quaternary carbon stereocenters are formed in high enantiomeric excess, and the conditions tolerate a range of substitution on both the oxindole and the aryl/vinyl coupling partners.
SOMBRÍO PANORAMA DE LA BANCA ANTE LA NULA REACTIVACIÓN DEL CRÉDITO (2001)

PARA LA BANCA EN MEXICO EL FUTURO INMEDIATO ES SOMBRIO ANTE LA CARENCIA DE DINERO, NULA REACTIVACION DEL CREDITO Y LA FALTA DE DEMANDA QUE OBEDECE A LAS ALTAS TASAS DE INTERES, AL TIEMPO QUE SE INCREMENTAR  LA LUCHA ENTRE LAS INSTITUCIONES EN LA OFERTA DE SUS SERVICIOS, AFIRMO VICTOR MANUEL COLINA RUBIO, DE LA FACULTAD DE ECONOMIA, DURANTE LA CONFERENCIA COMPRA DE BANCOS. EL INVESTIGADOR UNIVERSITARIO SE REFIRIO A LA VENTA DE BANCRECER A BANORTE, EL CUAL, DIJO, COMPRO CHATARRA, YA QUE EL COSTO DE SANEAMIENTO DEL PRIMERO SER  M S ALTO QUE LOS MIL 650 MILLONES DE PESOS PAGADOS, AL TIEMPO QUE NO DESCARTO POSIBLES MANEJOS CORRUPTOS EN LA TRANSACCION. SUBRAYO QUE PARA BANORTE NO REPRESENTO UNA BUENA COMPRA, NO OBSTANTE LOS INTERESES QUE PODR N GENERARSE POR LOS PAGARES DEL FOBAPROA. ANTE TALES CIRCUNSTANCIAS, ENFATIZO, EN LA VENTA A BANORTE NADIE GANO PERO LA SOCIEDAD SI PERDIO, TODA VEZ QUE EL COSTO FISCAL, SI SE SUMAN LOS PAGARES, ASCIENDE A 534 MIL MILLONES DE PESOS. APUNTO QUE LA SOLUCION SER  ASUMIR LOS PASIVOS COMO DEUDA PUBLICA CON EL COSTO QUE ELLO REPRESENTA, O DEJAR LA INSTITUCION BANCARIA EN LAS CONDICIONES ACTUALES.
Deployment Strategies for Protected Long-Reach PON (2012)

The mass deployment of fiber access networks is probably the most important network upgrade strategy for operators over the coming decade. Next-generation networks, and in particular the long-reach passive optical network (LR-PON) solution, aim to increase the long-term economic viability and sustainability of fiber-to-the-premises (FTTP) deployment. The LR-PON solution achieves this by minimizing the number of nodes and the amount of electronic equipment required within the network. Since an LR-PON replaces the metro backhaul network, which is usually a protected part of the network, protecting the long-reach part of an LR-PON against failure becomes a critical issue that needs to be taken into account. In this paper, we introduce a novel protection mechanism which, by spreading the load generated by a node failure over the network, can significantly reduce the overall protection capacity required. We then present a practical FTTP deployment scenario based on our protected LR-PON architecture for a European country. The problem is modeled using integer linear programming, and the optimization results, obtained using a real dataset provided by a national operator, show that a small number of metro/core nodes can provide protected connection to FTTP users. By applying a detailed cost model to the outcome of the optimization, we are able to show that our LR-PON deployment strategy, which minimizes the overall protection capacity, rather than just minimizing fiber distances in the LR-PON, can significantly reduce costs.
Paradoxical prognostic impact of TERT promoter mutations in gliomas depends on different histological and genetic backgrounds (2017)

We describe the TERT promoter mutation spectrum according to the histological, genetic, and molecular subtypes of glioma, which may aid in glioma subtype classification and have clinical implications.
5-HT4Receptors Are Not Involved in the Effects of Fluoxetine in the Corticosterone Model of Depression (2021)

Clinical and preclinical studies report the implication of 5-hydroxytryptamine 4 receptors (5-HT<sub>4</sub>Rs) in depression and anxiety. Here, we tested whether the absence of 5-HT4Rs influences the response to the antidepressant fluoxetine in mice subjected to chronic corticosterone administration, an animal model of depression and anxiety. Therefore, the effects of chronic administration of fluoxetine in corticosterone-treated wild-type (WT) and 5-HT<sub>4</sub>R knockout (KO) mice were evaluated in the open-field and novelty suppressed feeding tests. As 5-HT<sub>1A</sub> receptor (5-HT<sub>1A</sub>R) and brain-derived neurotrophic factor (BDNF) are critically involved in depression and anxiety, we further evaluated 5-HT<sub>1A</sub> receptor functionality by [<sup>35</sup>S]GTPγS autoradiography and BDNF mRNA expression by <i>in situ</i> hybridization techniques. We found that 5-HT<sub>4</sub>R KO and WT mice displayed anxiety- and depressive-like behavior following chronic administration of corticosterone, as evidenced in the open-field and novelty suppressed feeding tests. In the open-field, a decreased central activity was observed in naı̈ve and corticosterone-treated mice of both genotypes following chronic fluoxetine administration. In the novelty suppressed feeding test, a predictive paradigm of antidepressant activity, chronic treatment with fluoxetine reverted the latency to eat in both genotypes. The antidepressant also potentiated the corticosterone-induced desensitization of the 5-HT<sub>1A</sub>R in the dorsal raphe nucleus. Further, chronic fluoxetine increased BDNF mRNA expression in the dentate gyrus of the hippocampus in corticosterone-treated mice of both genotypes. Therefore, our findings indicate that the behavioral effects of fluoxetine in the corticosterone model of depression and anxiety appear not to be dependent on 5-HT<sub>4</sub>Rs.
Patterns of alternative splicing of fibronectin pre-mRNA in human adult and fetal tissues (1989)

Alternative splicing of fibronectin pre-mRNA at two distinct regions, termed ED-A and IIICS, was investigated with human adult and fetal tissues by the nuclease S1 protection assay. A clear tissue specificity was observed in the splicing pattern at the ED-A region. More ED-A+ than ED-A- mRNAs were identified in lung, whereas ED-A- mRNAs were predominantly expressed in liver. Endometrium contained nearly equal amounts of ED-A+ and ED-A- mRNAs. The splicing pattern at the ED-A region was also different between adult and fetal liver but not between adult and fetal lung. Tissue type specific splicing was also observed at the IIICS region. Although the mRNA species containing the complete IIICS sequence comprised 40-65% of the total fibronectin mRNAs irrespective of tissue types, expression of the mRNA species lacking a part or all of the IIICS sequence was more pronounced in adult liver than in other tissues including fetal liver. These results strongly suggest that the alternative splicing of fibronectin pre-mRNA in vivo is regulated in a tissue type specific manner at both the ED-A and IIICS regions and that it is developmentally regulated in liver but not in lung. On the basis of these and other observations reported previously, a possibility that a part of the fibronectins synthesized and secreted by hepatocytes is deposited in the tissue matrix is discussed.
Scattering Lens Resolves Sub-100 nm Structures with Visible Light (2011)

The smallest structures that conventional lenses are able to optically resolve are of the order of 200 nm. We introduce a new type of lens that exploits multiple scattering of light to generate a scanning nanosized optical focus. With an experimental realization of this lens in gallium phosphide we imaged gold nanoparticles at 97 nm optical resolution. Our work is the first lens that provides a resolution better than 100 nm at visible wavelengths.
Population pharmacokinetics (PK) of riociguat in patients (pts) with renal and hepatic impairment (2014)

Background: Riociguat, an oral soluble guanylate cyclase stimulator, is approved for the treatment of pulmonary arterial hypertension and persistent/recurrent chronic thromboembolic pulmonary hypertension (CTEPH) after surgical treatment or inoperable CTEPH. Aims: To characterize the population PK (PopPK) of riociguat in pts with renal and hepatic impairment. Methods: Plasma and urine samples were collected from pts in four Phase I studies; two renal impairment (n=64) and two hepatic impairment (n=64) studies, including smokers (n=56) and non-smokers (n=72). A single oral dose of riociguat 1.0 or 0.5 mg was administered. Nonlinear mixed-effects modeling was used to develop a PopPK model for riociguat and its metabolite M1. A combined, two-compartment model for riociguat and M1 was developed. Riociguat and M1 clearance (CL) was split into a renal part (filtration and excretion) and a non-renal part; the non-renal part for riociguat was split into its metabolism to M1 and a remaining non-renal part. Results: The PK of riociguat was described well by a two-compartment model. Total riociguat CL (1.912 L/h) was dominated by its metabolism to M1 (1.2 L/h). Renal CL (0.242 L/h) was mainly determined by filtration (0.174 L/h). Renal impairment reduced riociguat and M1 renal CL. Smoking increased riociguat metabolism to M1 by 3.5-fold. No effect of hepatic function biomarkers and Child–Pugh classification was found on riociguat or M1 CL. Conclusions: Riociguat and M1 renal CL was reduced by renal impairment. Similar values for the non-renal (non-metabolism) CL of riociguat were estimated in all groups; non-renal CL was increased in smokers.
Data for: Dating a Martian Meteorite with 20 Ma Precision Using a Prototype In-Situ Dating Instrument (2020)

These two excel data tables contain the reduced CODEX LARIMS data required to reproduce our Zagami isochron diagrams for February and September 2019 data acquisitions. Each excel file has a header explaining each column. Each spot measurement on Zagami is recorded as a row, with a filename to allow us to identify the original raw data if needed, the date acquired, the measurement position on the sample, the integrated peak areas, the 1-sigma standard error uncertainties, and correlation coefficients.