gebhart/patrepeval huggingface dataset:
Patient-derived zebrafish xenografts of uveal melanoma reveal ferroptosis as a drug target (2022)

Abstract Uveal melanoma (UM) has a high risk to progress to metastatic disease with a median survival of 3.9 months after metastases detection, as metastatic UM responds poorly to conventional and targeted chemotherapy and is largely refractory to immunotherapy. Here, we present a patient-derived zebrafish UM xenograft model mimicking metastatic UM. Cells isolated from Xmm66 spheroids derived from metastatic UM patient material were injected into 2 days-old zebrafish larvae resulting in micro-metastases in the liver and caudal hematopoietic tissue. Metastasis formation could be reduced by navitoclax and more efficient by the combinations navitoclax/everolimus and flavopiridol/quisinostat. We obtained spheroid cultures from 14 metastatic and 10 primary UM tissues, which were used for xenografts with a success rate of 100%. Importantly, the ferroptosis-related genes GPX4 and SLC7A11 are negatively correlated with the survival of UM patients (TCGA: n = 80; Leiden University Medical Centre cohort: n = 64), ferroptosis susceptibility is correlated with loss of BAP1, one of the key prognosticators for metastatic UM, and ferroptosis induction greatly reduced metastasis formation in the UM xenograft model. Collectively, we have established a patient-derived animal model for metastatic UM and identified ferroptosis induction as a possible therapeutic strategy for the treatment of UM patients.
to the Antithrombotic Effects of Heparin (2016)

The relative importance of antithrombin and anti-factor Xa activities of heparin fractions required to achieve optimal antithrombotic effects is unknown. To study this. we measured the effects of standard heparin. an octasaccharide heparin fraction (anti-factor Xa activity only). and dermatan sulfate (antithrombin activity only) on the prevention of thrombosis and related this to their anticoagulant effects in vivo in rabbits. Thrombosis was measured as the incorporation of ‘25l-fibrinogen into tissue thromboplastin-induced thrombi using a Wessler-type model. Ex vivo changes in thrombin clotting time (TCT) were used as an index of antithrombin activity. and a chromogenic antifactor Xa assay was used to measure anti-factor Xa activity. In addition. the ability of the three sulfated polysaccharides to simultaneously inhibit the generation of thrombin activity and to enhance the inactivation of the factor Xa added to initiate thrombin generation in plasma R ECENT OBSERVATIONS in experimental animals suggest that some low molecular weight fragments of heparin have antithrombotic effects in animals with significantly reduced risk of hemorrhagic complications.’3 Low molecular weight heparins generally have higher anti-factor Xa activity than antithrombin activity in plasma,’� and it has been suggested that the higher anti-factor Xa activity is responsible for the antithrombotic effects of low molecular weight heparins. However, we and others have shown that derivatives of heparin with very high ratios of anti-factor Xa to antithrombin activity are poor antithrombotic agents in experimental animals.’� The present study was performed in an attempt to determine the relative importance of the antithrombin and anti-factor Xa activities of heparin to the antithrombotic properties of heparin. The following sulfated polysaccharides were evaluated: an octasaccharide heparin derivative with anti-factor Xa activity only; dermatan sulfate with antithrombin activity only5’6; and standard heparin with equivalent antifactor Xa to antithrombin activity. We also investigated the ability of the three agents to inhibit the generation of thrombin activity in plasma and to
ENVIRONMENTAL STUDIES ON THE IMPACT OF THE DRAINS EFFLUENT UPON THE SOUTHERN SECTOR OF LAKE MANZALAH, EGYPT (2001)

W, ffect of the drains effluent on the variations of trace metals and inorganic anions and cations of water were studied in the southern sector of Lake Manzalah during four successive seasons (1999-2000). The study revealed that an obvious depletion of dissolved oxygen has occurred in addition to a high increase in COD and BOD in the area which received the urban and agricultural wastes from Bahr El-Bagar drain. The concentrations of the nutrient salts showed a wide fluctuation and abrupt changes due to irregular influx of different wastes. Also, high and abnormal concentrations of ammonia, nitrate and orthophosphate were recorded. The levels of trace metals in lake water were high in areas received domestic and agricultural effluents.
Direct reprogramming of somatic cells into neural stem cells or neurons for neurological disorders (2015)

Direct reprogramming of somatic cells into neurons or neural stem cells is one of the most important frontier fields in current neuroscience research. Without undergoing the pluripotency stage, induced neurons or induced neural stem cells are a safer and timelier manner resource in comparison to those derived from induced pluripotent stem cells. In this prospective, we review the recent advances in generation of induced neurons and induced neural stem cells in vitro and in vivo and their potential treatments of neurological disorders.
Nano-objects emitted during maintenance of common particle generators: direct chemical characterization with aerosol mass spectrometry and implications for risk assessments Patrik T. NilssonChristina IsaxonAxel C. ErikssonMaria E. Messing • Linus LudvigssonJenny RisslerMaria HedmerHakan Tinnerberg • Anders GudmundssonKnut DeppertMats BohgardJoakim H. Pagels (2013)

Nanotechnology gives us materials with enhanced or completely new properties. At the same time, inhalation of manufactured nano-objects has been related to an array of adverse biological effects. We characterized particle emissions, which occurred during maintenance of common metal nanoparticle generators and contrasted the properties of the emitted particles with those originally produced by the gen- erators. A new approach using online aerosol mass spectrometry (AMS), for time- and size-resolved measurements of the particle chemical composition, was applied in combination with more conventional techniques for particle sampling and analysis, includ- ing electron microscopy. Emissions during mainte- nance work, in terms of mass and surface area concentration in the size range of 0.02-10 lm, were dominated by large agglomerates (1-5 lm). With AMS, we show that the particle composition depends on both generator type and maintenance task being performed and that the instrument can be used for highly time-resolved selective studies of metal nano- particle emissions. The emitted agglomerates have a relatively high probability to be deposited in the lower respiratory tract, since the mean particle diameter coincided with a peak in the lung deposition curve. Each of these agglomerates consisted of a very high number (10 3 -10 5 /agglomerate) of nanometer-sized primary particles originating from the particle synthe- sis process. This made them possess large surface areas, one of the key properties in nanotoxicology. Similar agglomerates may be emitted in a wide range of processes when nanoparticles are manufactured or handled. The fate of such agglomerates, once
Jointly Channel Assignment and Power Allocation Algorithm Based on Utility Optimization for Wireless Mesh Networks (2011)

该文针对多信道无线mesh网络，采用基于效用最优的定价机制，提出了一种功率-干扰价格模型，并基于该模型提出了一种分布式联合信道分配和功率分配算法。每个节点根据自己所消耗功率状况合理地定功率价格，并根据自己所受干扰状况合理地定干扰价格。通过功率价格和干扰价格来调节链路的信道分配和功率分配，使网络效用最大化。仿真结果表明：所提出的算法能够快速、平稳地收敛到近似最优解。同时还仿真了网络可用信道数目、节点射频数目和功率对系统性能的影响，可以为网络配置提供参考。
Proliferation and migration of endothelial cells is promoted by endothelins via activation of ETB receptors (1995)

The growth and migration of endothelial cells are the prerequisites for vascular remodeling. The effects of endothelin (ET)-1 and -3 (ET-1, ET-3) on the proliferation and migration of endothelial cells isolated from bovine adrenal capillaries (BACE) and human umbilical veins (HUVEC) have been investigated. Cell proliferation (measured as DNA synthesis and as total cell number) and migration were significantly increased by ET-1 and ET-3. Dose-dependent proliferation was produced by ET-1 and ET-3 in both cell lines, with maximal effects at 0.1 nM concentration. ET-1 and ET-3 also stimulated BACE and HUVEC mobilization in a dose-dependent manner. The maximal responses were obtained at 10 nM concentration in both BACE and HUVEC. The full agonist for the ETB receptor, ET-(16--21), was able to reproduce the effects of ET on proliferation and migration of both cell lines. Modification of ET-(16--21) at Leu17 and Ile19 and amidation of the COOH-terminal were accompanied by loss of activity. The ETB-receptor antagonist IRL-1038 blocked the migration induced by ET-3 and ET-(16--21), whereas the ETA-receptor antagonist BQ-123 was not effective. We conclude that ETs, by favoring endothelial cell growth and mobilization, can contribute to neovascularization through an autocrine mechanism that requires ETB-receptor activation.
Clinical and angiographic predictors of operative mortality from the collaborative study in coronary artery surgery (CASS). (1981)

Fifteen institutions participating in the Collaborative Study in Coronary Artery Surgery (CASS) have performed isolated coronary artery bypass surgery upon 6630 patients (1061 women and 5569 men) for coronary artery disease. The overall operative mortality (OM) was 2.3% (range 0.3-6.4%). Mortality increased with age, from 0 in the group 20-29 years old to 7.9% in the group 70 years and older. OM was higher for women in each group, ranging from 2.8% for ages 30-39 years to 12.3% for age 70 years and older (0.8% and 5.8% for men). Clinical manifestations of congestive heart failure were associated with increased OM. Mortality was 1.4% in one-vessel, 2.1% in two-vessel and 2.8% in three-vessel disease (diameter narrowing greater than or equal to 70%). Among 1019 patients with left main coronary artery (LMCA) stenosis, OM ranged from 1.6% in patients with mild stenosis and a right-dominant system to 25% in patients with severe (greater than or equal to 90%) stenosis and left dominance. OM varied with ejection fraction (EF) (1.9% for EF greater than or equal to 50% to 6.7% for EF less than 19%) and left ventricular wall motion score (1.7% for least abnormal to 9.1% for most abnormal). For elective surgery, OM was 1.7%, for urgent surgery 3.5%, and for emergency surgery 10.8%. Mortality was 40.0% among 30 patients with severe LMCA stenosis who underwent emergency revascularization. Advanced age, female sex, symptoms of heart failure, LMCA stenosis, impaired left ventricular function and nonelective surgery are associated with a higher OM. These factors should be considered in the selection of patients for coronary artery surgery.
Governance of Clubs and Firms with Cultural Dimensions (1999)

The neoclassical way to cope with firms providing services, or with clubs procuring services, is restricted by the lack of institutional features. An institutional approach is introduced that requires a cooperative governance to realize the potential value-production by firms, or to realize the potential user-value by clubs. For each, a distinctive governance system is introduced. The firm requires an implementation governance to activate the value-production capacities of its service providers. It is empowered top-down by the unique top- position of the organization. The club, on the other hand, requires a representation governance to aggregate the user-values of its members for some common service and to order this service. It is empowered bottom-up by the service-users, i.e., the members of the club using that common service. Institutional characteristics are also re ected in the distribution functions that are used inrewarding positions in firms and clubs. Some cultural dimensions are expressed in these distribution functions. That allows us to relate characteristics of governance systems to society's cultural dimensions.
VEGF-C/VEGFR-3 signalling in macrophages ameliorates acute lung injury (2021)

VEGF-C/VEGFR-3 signalling in macrophages ameliorates experimental lung injury. This mechanism may also provide an explanation for ARDS resolution.
Recommendations on Filtering of IPv4 Packets Containing IPv4 Options (2014)

This document provides advice on the filtering of IPv4 packets based
on the IPv4 options they contain. Additionally, it discusses the
operational and interoperability implications of dropping packets
based on the IP options they contain.
Dynamically negotiated resource management for data intensive application suites (2000)

In contemporary computers and networks of computers, various application domains are making increasing demands on the system to move data from one place to another, particularly under some form of soft real-time constraint. A brute force technique for implementing applications in this type of domain demands excessive system resources, even though the actual requirements by different parts of the application vary according to the way it is being used at the moment. A more sophisticated approach is to provide applications with the ability to dynamically adjust resource requirements according to their precise needs, as well as the availability of system resources. This paper describes a set of principles for designing systems to provide support for soft real-time applications using dynamic negotiation. Next, the execution level abstraction is introduced as a specific mechanism for implementing the principles. The utility of the principles and the execution level abstraction is then shown in the design of three resource managers that facilitate dynamic application adaptation: Gryphon, EPA/RT-PCIP, and the DQM architectures.
Anthocyanin and Proanthocyanidin Content in Selected White and Red Wines. Oxygen Radical Absorbance Capacity Comparison with Nontraditional Wines Obtained from Highbush Blueberry (2003)

Antioxidant capacity, as measured by oxygen radical absorbance capacity (ORAC(PE)), total phenolic, total and individual anthocyanins, and proanthocyanidin fraction contents were evaluated in red and white wines from grapes. A comparison in terms of antioxidant capacity is made with nontraditional wines made from highbush blueberry. Blueberries are among fruits that are best recognized for their potential health benefits. In red wines, total oligomeric proanthocyanidin content, including catechins, was substantially higher (177.18 +/- 96.06 mg/L) than that in white wines (8.75 +/- 4.53 mg/L). A relative high correlation in red wines was found between ORAC(PE) values and malvidin compounds (r = 0.75, P < 0.10), and proanthocyanidins (r = 0.87, P < 0.05). In white wines, a significant correlation was found between the trimeric proanthocyanidin fraction and peroxyl radical scavenging values (r = 0.86, P < 0.10). A moderate drink (1 drink per day, about 140 mL) of red wine, or white wine, or wine made from highbush blueberry corresponds to an intake of 2.04 +/- 0.81 mmol of TE, 0.47 +/- 0.15 mmol of TE, and 2.42 +/- 0.88 mmol of TE of ORAC(PE)/day, respectively.
Abnormal Function of Endocrine Pancreas and Anterior Pituitary in Friedreich's Ataxia (1978)

A family had three siblings affected with classic Friedreich's ataxia. One sibling died at age 20 with fulminant diabetic ketoacidosis. The other two affected siblings are identical twin sisters without clinical diabetes but with an abnormality in the metabolism of exogenously administered glucose. These twins also have abnormal hypothalamic-pituitary control of prolactin and possibly of growth-hormone secretion. This study extends the previous reports of endocrine deficienceis associated with Friedreich's ataxia. The mechanisms underlying this association are undetermined but could represent pleiotropic effects of the Friedreich's ataxia gene or secondary manifestations of the primary central nervous system degeneration, or both.
Identification of the mRNA and polypeptide subunit for prostaglandin endoperoxide synthase from mouse mastocytoma P-815 cells (1983)

Cloned mouse mastocytoma P-815.2-E-6 cells are barely able to synthesize prostaglandins because of a lack of prostaglandin endoperoxide synthase activity. However, the addition of sodium n-butyrate at 1 mM induces synthesis de novo of prostaglandins in this cell line. Employing this system, we could isolate an mRNA for prostaglandin endoperoxide synthase by a combination of cell-free translation and immunoprecipitation. The antibody, prepared in rabbit by injecting purified prostaglandin endoperoxide synthase from bovine vesicular gland, was shown to cross-react with the corresponding enzyme from 2-E-6 cells. The poly(A)-containing mRNA has a sedimentation coefficient of 17S and codes for a single polypeptide chain of Mr 62 000 as estimated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The Mr of the mouse polypeptide chain appears very similar to that of the purified carbohydrate-free prostaglandin endoperoxide synthase from sheep vesicular gland. These findings are a contribution to the isolation of the gene for prostaglandin endoperoxide synthase.
Leader-following scaled consensus of second-order multi-agent systems under directed topologies (2019)

This paper investigates the leader-following scaled consensus problem of second-order multi-agent systems under directed topologies. Three novel leader-following scaled consensus protocols are designed. First, a novel scaled consensus protocol is proposed. It can guarantee the velocity of each agent in one sub-group exactly follow that of a leader, and the follower agents achieve scaled consensus. Second, another proposed protocol enables the agents' positions and velocities of one sub-group accurately track those of a leader, and the follower agents achieve scaled consensus. Third, consider the case where the leader's states available to one or multiple followers and the leader travels with a varying velocity, a novel scaled consensus tracking protocol is proposed. Sufficient and necessary conditions are obtained to guarantee scaled consensus tracking for the three cases,respectively. Finally, simulation examples are made to verify the effectiveness of the theoretical results.
Shock Severity Modifies Associations Between RBC Transfusion in the First 48 Hours of Sepsis Onset and the Duration of Organ Dysfunction in Critically Ill Septic Children* (2020)

In agreement with previous studies, early RBC transfusion was independently associated with longer duration of organ dysfunction. Ours is among the first studies to document different transfusion-associated risks based on clinically available measures of shock severity, demonstrating greater transfusion-associated risks in children with less severe shock. Larger multicenter studies to verify these interaction effects are essential to plan much-needed RBC transfusion trials for critically ill septic children.
Increased Brain Glucose Uptake After 12 Weeks of Aerobic High-Intensity Interval Training in Young and Older Adults (2017)

We demonstrate that brain glucose metabolism increased after 12 weeks of HIIT in adults in regions where it is reduced in Alzheimer's disease.
Sensing OFDM Signal: A Deep Learning Approach (2019)

Spectrum sensing plays a critical role in dynamic spectrum sharing, a promising technology to address the radio spectrum shortage. In particular, sensing of orthogonal frequency division multiplexing (OFDM) signals, a widely accepted multi-carrier transmission paradigm, has received paramount interest. Despite various efforts, noise uncertainty, timing delay and carrier frequency offset (CFO) still remain as challenging problems, significantly degrading the sensing performance. In this work, we develop two novel OFDM sensing frameworks utilizing the properties of deep learning networks. Specifically, we first propose a stacked autoencoder based spectrum sensing method (SAE-SS), in which a stacked autoencoder network is designed to extract the hidden features of OFDM signals for classifying the user’s activities. Compared to the conventional OFDM sensing methods, SAE-SS is significantly superior in the robustness to noise uncertainty, timing delay, and CFO. Moreover, SAE-SS requires neither any prior information of signals (e.g., signal structure, pilot tones, cyclic prefix) nor explicit feature extraction algorithms which however are essential for the conventional OFDM sensing methods. To further improve the sensing accuracy of SAE-SS, especially under low SNR conditions, we propose a stacked autoencoder based spectrum sensing method using time-frequency domain signals (SAE-TF). SAE-TF achieves higher sensing accuracy than SAE-SS using the features extracted from both time and frequency domains, at the cost of higher computational complexity. Through extensive simulation results, both SAE-SS and SAE-TF are shown to achieve notably higher sensing accuracy than that of state of the art approaches.
A modified PAP (peroxidase-anti-peroxidase) staining technique using sera from patients with dengue hemorrhagic fever (DHF): 4 step PAP staining technique. (1979)

BHK-21 cells infected with dengue virus type 1 were stained by a newly developed 4 step PAP (peroxidase-anti-peroxidase) technique using sera from patients with dengue hemorrhagic fever as anti-virus antibody. The intensity of staining of the sera was proportional to the hemagglutination inhibition and neutralization titers. With this new technique using sera from patients it should be possible to use the PAP technique of virus infections.
Rhein inhibits glucose uptake in Ehrlich ascites tumor cells by alteration of membrane-associated functions (1993)

Rhein (RH), 4,5 dihydroxyanthraquinone-2-carboxylic acid, is known to inhibit the glycolysis of neoplastic cells by impairing glucose uptake. In order to establish whether this might be due to a selective interaction of the carrier with the drug or to functional modifications of the cell membrane, the effect of RH on glucose uptake in Ehrlich ascites tumor cells has been investigated. RH strongly inhibits the uptake of both 2-deoxyglucose and 3-O-methylglucose, so the reduced influx therefore cannot be ascribed to an effect on glucose phosphorylation. The inhibition of glucose transport does not depend on a reduction of the number of the carriers as indicated by the inability of the drug to interfere with the synthesis of the transporter. Moreover, the extent of total binding of cytochalasin B, as well as the fact that glucose specificity is not altered, indicate that the intrinsic activity of the glucose carrier is not affected. We therefore conclude that the inhibition of glucose uptake must be ascribed to an interaction of the drug with cell membranes that results in an alteration of membrane-associated functions.
The response of BWR Mark II containments to station blackout severe accident sequences (1991)

This report describes the results of a series of calculations conducted to investigate the response of BWR Mark 2 containments to short-term and long-term station blackout severe accident sequences. The BWR-LTAS, BWRSAR, and MELCOR codes were employed to conduct quantitative accident sequence progression and containment response analyses for several station blackout scenarios. The accident mitigation effectiveness of automatic depressurization system actuation, drywell flooding via containment spray operation, and debris quenching in Mark 2 suppression pools is assessed. 27 refs., 16 figs., 21 tabs.