gebhart/patrepeval huggingface dataset:
Purification and characterization of catechol 1,2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 and cloning and sequencing of its catA gene (1998)

A method was developed for the purification of catechol 1, 2-dioxygenase from Rhodococcus rhodochrous NCIMB 13259 that had been grown in the presence of benzyl alcohol. The enzyme has very similar apparent Km (1-2 microM) and Vmax (13-19 units/mg of protein) values for the intradiol cleavage of catechol, 3-methylcatechol and 4-methylcatechol and it is optimally active at pH9. Cross-linking studies indicate that the enzyme is a homodimer. It contains 0.6 atoms of Fe per subunit. The enzyme was crystallized with 15% (w/v) poly(ethylene glycol) 4000/0.33 M CaCl2/25 mM Tris (pH7.5) by using a microseeding technique. Preliminary X-ray characterization showed that the crystals are in space group C2 with unit-cell dimensions a=111.9 A, b=78.1 A, c=134.6 A, beta=100 degrees. An oligonucleotide probe, made by hemi-nested PCR, was used to clone the gene encoding catechol 1,2-dioxygenase (catA). The deduced 282-residue sequence corresponds to a protein of molecular mass 31539 Da, close to the molecular mass of 31558 Da obtained by electrospray MS of the purified enzyme. catA was subcloned into the expression vector pTB361, allowing the production of catechol 1,2-dioxygenase to approx. 40% of the total cellular protein. The deduced amino acid sequence of the enzyme has 56% and 75% identity with the catechol 1, 2-dioxygenases of Arthrobacter mA3 and Rhodococcus erythropolis AN-13 respectively, but less than 35% identity with intradiol catechol and chlorocatechol dioxygenases of Gram-negative bacteria.
Coherent laser scanning diffraction microscopy (2009)

Coherent diffractive imaging (CDI) is a promising approach to high-resolution x-ray microscopy. While CDI typically has a rather limited field of view, this problem can be solved by ptychography, a technique for which an extended object is raster scanned by a compact coherent illumination probe. Significant overlap of illumination for adjacent scan points allows then a self-consistent reconstruction from the entirety of collected coherent diffraction patterns. However, current reconstruction schemes require accurate &lt;em&gt;a priori&lt;/em&gt; knowledge of the probe. Our recently developed new algorithm for ptychographic data sets allows us to simultaneously reconstruct both object and illuminating probe. We demonstrate the application of the new method in a test experiment with visible laser light showing that intricate illumination functions can be retrieved reliably.
Decoupled Thermo‐ and pH‐Responsive Hydrogel Microspheres Cross‐Linked by Rotaxane Networks (2017)

Rotaxane cross-linked (RC) microgels that exhibit a decoupled thermo- and pH-responsive volume transition were developed. The pH-induced changes of the aggregation/disaggregation states of cyclodextrin in the RC networks were used to control the swelling capacity of the entire microgels. Different from conventional thermo- and pH-responsive microgels, which are usually obtained from copolymerizations involving charged monomers, the RC microgels respond to temperature as intended, even in the presence of charged functional molecules such as dyes in the microgel dispersion. The results of this study should lead to new applications, including drug delivery systems that require a retention of their smart functions even in environments that may contain foreign ions, for example, in in vivo experiments.
Leakage of Dataset Properties in Multi-Party Machine Learning (2020)

Secure multi-party machine learning allows several parties to build a model on their pooled data to increase utility while not explicitly sharing data with each other. We show that such multi-party computation can cause leakage of global dataset properties between the parties even when parties obtain only black-box access to the final model. In particular, a ``curious'' party can infer the distribution of sensitive attributes in other parties' data with high accuracy. This raises concerns regarding the confidentiality of properties pertaining to the whole dataset as opposed to individual data records. We show that our attack can leak population-level properties in datasets of different types, including tabular, text, and graph data. To understand and measure the source of leakage, we consider several models of correlation between a sensitive attribute and the rest of the data. Using multiple machine learning models, we show that leakage occurs even if the sensitive attribute is not included in the training data and has a low correlation with other attributes or the target variable.
John Harley. Orlando Gibbons and the Gibbons Family of Musicians. Brookfield, Vt.: Ashgate. 1999. Pp. x, 341. $78.95. ISBN 1-84014-209-X. (2001)

An abstract is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
Pramipexole Reduces Phosphorylation of α-Synuclein at Serine-129 (2013)

α-Synuclein is a central component of the pathogenesis of Parkinson's disease (PD). Phosphorylation at serine-129 represents an important post-translational modification and constitutes the major form of the protein in Lewy bodies. Several kinases have been implicated in the phosphorylation of α-synuclein. The targeting of kinase pathways as a potential to influence the pathogenesis of PD is an important focus of attention, given that mutations of specific kinases (LRRK2 and PINK1) are causes of familial PD. Pramipexole (PPX) is a dopamine agonist developed for the symptomatic relief of PD. Several in vitro and in vivo laboratory studies have demonstrated that PPX exerts neuroprotective properties in model systems of relevance to PD. The present study demonstrates that PPX inhibits the phosphorylation of α-synuclein and that this is independent of dopamine receptor activation. PPX blocks the increase in phosphorylated α-synuclein induced by inhibition of the ubiquitin proteasomal system. The phosphorylation of α-synuclein occurs in part at least through casein kinase 2, and PPX in turn reduces the phosphorylation of this enzyme, thereby inhibiting its activity. Thus, PPX decreases the phosphorylation of α-synuclein, and this mechanism may contribute to its protective properties in PD models.
Single-mode fibre coupler with the 3-dB splitting ratio simultaneously at 0.83 and 1.3 μm (2004)

The problem of designing a two-wave single-mode-fibre 3-dB coupler is considered. The method is proposed for adjusting the parameters of the coupler for simultaneous use of radiation at 0.83 and 1.3 μm in one fibre interferometer. The dependence of the coupling efficiency on the wavelength is studied experimentally for different angle mismatches of fibres in the coupler. The results of experiments and numerical calculations are presented.
Transmission zero engineering in lateral double-barrier resonant tunneling devices (1996)

Transmission zero engineering in lateral double-barrier resonant tunneling devices is investigated. We show that, by inserting a resonant cavity in the quantum well region of a lateral double-barrier resonant tunneling structure and engineering the placement of transmission zero-pole pairs, the current peak-to-valley ratio of the device can be drastically improved at low temperature. An advantage of this structure also is that a lower peak voltage can be obtained compared to the corresponding lateral double-barrier resonant tunneling device.
The 5' non-coding region of the BCR/ABL oncogene augments its ability to stimulate the growth of immature lymphoid cells. (1991)

The Philadelphia chromosome (Ph1, t9:22;34:q11) is a reciprocal translocation between chromosome 22 and chromosome 9 which results in the formation of the chimeric BCR/ABL oncogene. Alternative forms of BCR/ABL are produced by splicing different sets of exons of the BCR gene to a common set of c-ABL sequences. This results in the formation of an 8.7 kilobase mRNA that encodes the P210 BCR/ABL gene product or a 7.0 kilobase mRNA that encodes the P185 BCR/ABL gene product. Both BCR/ABL transcripts derive their 5' non-coding sequences from the BCR gene locus. This 5' region is over 500 nucleotides in length, has a GC content greater than 75% and has a short open reading frame. To determine if this unusual 5' non-coding region plays a role in BCR/ABL transformation, we prepared retroviral vectors containing identical BCR/ABL coding regions but differing in the length of the BCR 5' non-coding region. Matched viral stocks were evaluated for their ability to transform bone marrow in vitro and for their ability to cause tumors when inoculated into 3- to 4-week-old mice. In this report we present the unexpected finding that the BCR/ABL 5' non-coding region augments the transforming activity of both P210 and P185 BCR/ABL in vitro. In vivo, BCR/ABL is a weak tumorigenic agent and its potency is enhanced by the presence of the 5' non-coding region.
ChemInform Abstract: A Novel Indole‐Diterpenoid, JBIR‐03, with anti‐MRSA Activity from Dichotomomyces cejpii var. cejpii NBRC 103559. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A 100mW 9.6Gb/s Transceiver in 90nm CMOS for Next-Generation Memory Interfaces (2006)

An architecture for next-generation memory interface is demonstrated using 90nm bulk silicon to provide a 2-tap emphasized TX with <19ps jitter at 9.6Gb/s. The circuit uses a programmable PLL to track jitter up to 200MHz. The transceiver consumes 100mW from a 1V supply
Multilayer coating design achieving a broadband 90° phase shift (1980)

For nonnormal incidence angles, it has been shown that the polarization state may be controlled through the use of specially designed multilayer coatings. An optimization technique has been used to determine layer thicknesses for a coating design that produces a 90 degrees phase shift between the p- and s-polarization components over a wavelength range of Deltalambda/lambda(0) = +/-5% while maintaining high reflectivities for both components. A tolerance analysis indicates that the coating layers must be deposited within +/-1% to achieve a +/-3 degrees phase shift error over the spectral range of the design.
A new template encapsulation strategy for larger cavity metal ion cages (1994)

Stereospecific sequential condensation of paraformaldehyde and propionaldehyde with a tripodal bis(triamine)cobalt(III) template rapidly encapsulates the metal, and a subsequent facile reduction of imine functions gives an expanded cavity hexaazabicyclic cage system with unusual structural and chromophore electron properties, complemented by an exceptional stability and relatively fast CoII/III electron exchange rates.
SU‐F‐T‐24: Impact of Source Position and Dose Distribution Due to Curvature of HDR Transfer Tubes (2016)

Purpose: Brachytherapy is a highly targeted from of radiotherapy. While this may lead to ideal dose distributions on the treatment planning system, a small error in source location can lead to change in the dose distribution. The purpose of this study is to quantify the impact on source position error due to curvature of the transfer tubes and the impact this may have on the dose distribution. Methods: Since the source travels along the midline of the tube, an estimate of the positioning error for various angles of curvature was determined using geometric properties of the tube. Based on the range of values a specific shift was chosen to alter the treatment plans for a number of cervical cancer patients who had undergone HDR brachytherapy boost using tandem and ovoids. Impact of dose to target and organs at risk were determined and checked against guidelines outlined by radiation oncologist. Results: The estimate of the positioning error was 2mm short of the expected position (the curved tube can only cause the source to not reach as far as with a flat tube). Quantitative impact on the dose distribution is still in the process of being analyzed. Conclusion: The accepted positioning tolerance for the source position of a HDR brachytherapy unit is plus or minus 1mm. If there is an additional 2mm discrepancy due to tube curvature, this can result in a source being 1mm to 3mm short of the expected location. While we do always attempt to keep the tubes straight, in some cases such as with tandem and ovoids, the tandem connector does not extend as far out from the patient so the ovoid tubes always contain some degree of curvature. The dose impact of this may be significant.
Supplementary Table 3 from Integrative Analysis of Genomic Aberrations Associated with Prostate Cancer Progression (2023)

Supplementary Table 3 from Integrative Analysis of Genomic Aberrations Associated with Prostate Cancer Progression
Axial Mixing and Selectivity. Two Consecutive First-Order Reactions (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleAxial Mixing and Selectivity. Two Consecutive First-Order ReactionsC. B. RosasCite this: Ind. Eng. Chem. Fundamen. 1969, 8, 2, 361–364Publication Date (Print):May 1, 1969Publication History Published online1 May 2002Published inissue 1 May 1969https://pubs.acs.org/doi/10.1021/i160030a031https://doi.org/10.1021/i160030a031research-articleACS PublicationsRequest reuse permissionsArticle Views96Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Flexible Brillouin Bandwidth Broadening for an Amplification, Filtering or Millimeter Wave Generation Systems (2006)

In this paper a simple method for the tunable bandwidth enhancement of Stimulated Brillouin Scattering is presented. It is based on the simultaneous use of several modulated pump sources in the system.
Power efficiency analysis of a multi-oscillated current resonant type DC-DC converter (2008)

This paper deals with an analysis of the power efficiency of a multi-oscillated current resonant type DC-DC converter. The current resonant type converter employs generally the pulse frequency modulation. For this reason, the magnetizing current through the converter causes not only a power loss under a light load, but also a loss during stand-by. In order to solve these problems, a multi-oscillated current resonant type DC-DC converter has been proposed, and revealed the advantage of its control method which can reduce power loss under light load and keep low switching noise. An analytical relationship of among states, operating mode and efficiency characteristics of this converter are defined. As a result, it was confirmed that for this converter, the output power depends on the voltage of resonant capacitor, and consequently, it is important to determine constants of resonant capacitor and inductance of transformer. The maximum efficiency is 95.4% with the magnetizing inductance 1.8 mH.
Expression, purification and biochemical characterization of a recombinant phospholipase A2, with anticoagulant activity from Agkistrodon halys Pallas. (2001)

A cloned cDNA encoding a PLA2 from Agkistrodon halys Pallas was found to have conservative residues Glu53 and Trp70 but with Lys56 and Lys67 substituted by Thr56 and Asp67, respectively, when compared with sequences of other class II PLA2 with anticoagulant activity. It was inserted into a temperature-sensitive bacterial expression vector and effectively expressed in Escherichia coli RR1. The protein was produced as insoluble inclusion bodies and recovered by centrifugation after enzyme digestion. By washing to partial purification, the expression product was refolded and was purified by FPLC superose 12 to appear as a single band in SDS-PAGE. The recombinant protein proved to have obvious enzymatic, anticoagulant and hemolytic activities, which were removed after modification by p-BPB. These findings suggest that the pharmacological activities of this recombinant PLA2 may be related to its catalytic activity and warrant further research on the structure-function relationships of the pharmacological site of the PLA2 from Agkistrodon halys Pallas.
Performance analysis of UWB radar for vehicle in multi-user environments (2008)

This paper analyzes the performance of UWB radar for vehicle in multi-user environments. To evaluate the probability of detection in UWB vehicular radar under a multi-user environment, we use the transmitter and receiver architecture of UWB radar system model. The system model consists of three major parts: a transmitter, multi-path channel and a receiver. Finally, the characteristics of vehicle-mounted UWB radar are evaluated by simulation. Simulation results show that the detection probability is reached with 1 in AWGN channel when relative distance is 120 m and the probability of detection becomes 0.55 when relative distance is 47 m in the IEEE 802.15.4a CM1.
Downsizing Proto-oncogene cFos to Short Helix-Constrained Peptides That Bind Jun (2017)

The oncogenic transcription factor activator protein-1 (AP-1) is a DNA-binding protein that assembles through dimerization of Fos and Jun protein subunits, their leucine-rich helical sequences entwining into a coiled-coil structure. This study reports on downsizing the proto-oncogene cFos protein (380 residues) to shorter peptides (37-25 residues) modified with helix-inducing constraints to enhance binding to Jun. A crystal structure is reported for a 37-residue Fos-derived peptide (FosW) bound to Jun. This guided iterative downsizing of FosW to shorter peptide sequences that were constrained into stable water-soluble α-helices by connecting amino acid side chains to form cyclic pentapeptide components. Structural integrity in the presence and absence of Jun was assessed by circular dichroism spectroscopy, while the thermodynamics of binding to cFos was measured by isothermal titration calorimetry. A 25-residue constrained peptide, one-third shorter yet 25% more helical than the structurally characterized 37-residue Fos-derived peptide, retained 80% of the binding free energy as a result of preorganization in a Jun-binding helix conformation, with the entropy gain (TΔS = +3.2 kcal/mol) compensating for the enthalpy loss. Attaching a cell-penetrating peptide (TAT<sub>48-57</sub>) and a nuclear localization signal (SV40) promoted cell uptake, localization to the nucleus, and inhibition of the proliferation of two breast cancer cell lines.
Pulsed Laser Deposition of La0,67Ca0,33MnO3 thin films on LiNbO3 (2005)

Surface Acoustic Waves on piezoelectric substrates can be used to investigate the dynamic conductivity of thin films in a non-contact and very sensitive way, especially at low conductivities. Here, we report on such surface acoustic wave studies to characterize thin manganite film like La0.67Ca0.33MnO3, exhibiting a Jan Teller effect with a strong electron phonon interaction and a metal insulator transition at high temperatures. We report on the deposition of La0.67Ca0.33MnO3 on piezoelectric substrates (LiNbO3 in different crystal cuts) employing a pulsed laser deposition technique. The structural qualities of the thin films are examined by X-Ray Diffraction, Scanning Electron Microscope and Energy Dispersive X-ray spectroscopy. For the electrical characterization, we employ the surface acoustic wave technique, accompanied by conventional DC-resistance measurements for comparison.
Coal, gas and coal seam gas (2012)

This edition of Chain Reaction features several articles on the campaigns against coal seam gas (CSG) in eastern Australia. The first one, by Friends of the Earth campaigner Drew Hutton, describes the campaign that has seen farmers and environmentalists join together to forge a strong alliance aimed at stopping the spread of CSG. A fundamental element of the Lock the Gate (LTG) alliance is the call for land owners to 'lock the gate' and refuse access to mining companies.
A focal plane normal-flow measurement chip (2004)

In this paper, we present the algorithm and operation of an aVLSI chip that can extract normal optical flow by using the gradient approach without interfering with the imaging process. This approach is feasible for scaling to larger arrays without affecting the processing or the processing area. Our system has a 92 x 52 photosensitive array of APS pixels at the core with processing circuits on the periphery. We discuss the approach and the different blocks in the design and then demonstrate the working of the individual blocks and of the system as a whole. The chip outputs the image, the spatial and temporal gradients and the normal flow at the read-out frame-rate with no penalty to the imaging process. The chip occupies an area of 4.5 mm<sup>2</sup> and consumes 2.6 mW (at V<sub>dd</sub> = 5V). Once normal flow is obtained, the chip can be used to compute focus of expansion, time to contact and many other motion properties of images that can be used to control robots. Tracking systems can use the velocity and segmentation of moving objects can be realized using motion discontinuity.
Outsourced private set intersection using homomorphic encryption (2012)

Private set intersection enables two parties -- a client and a server -- to compute the intersection of their respective sets without disclosing anything else. It is a fundamental operation -- equivalent to a secure, distributed database join -- and has many applications particularly in privacy-preserving law enforcement. In this paper we present a novel protocol that has linear complexity, is secure in the malicious model without random oracles, is client set size-independent and efficient. Furthermore, the computation of the intersection can be outsourced to an oblivious service provider, as in secure cloud computing. We leverage a completely novel construction for computing the intersection using Bloom filter and homomorphic encryption. For outsourcing we require and introduce a new homomorphic encryption scheme which may be of independent interest.