gebhart/patrepeval huggingface dataset:
Development of a Quartz Sand Protocol for Exoproteome Exploration from Anammox Consortia (2020)

A critical prerequisite for unbiased anaerobic ammonium oxidation (anammox) exoproteome exploration is the optimal extraction and treatment of extracellular polymeric substances (EPS). Herein, by leveraging the advantages of physical shear and potential electrostatic repulsion mechanisms, a nondestructive quartz sand protocol for in situ extraction of extracellular proteins (PN) for anammox consortia was developed and applied. According to an L16(45) orthogonal design, the optimal procedure, which contributes the maximum yield of EPS with minimal cell rupture, was identified as follows: 1.0 mm particle size, 100 g/g-VSS quartz sand ratio, 400 rpm, and 2 h. While achieving approximately the same yield of EPS, hierarchical clustering and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the quartz sand extraction was highly similar to the conventional cation exchange resin (CER) treatment in protein secondary structure properties and protein band distribution patterns, compared to other methods. However, the number of identified proteins and hydrophobic protein proportions were significantly increased by 5.3-fold and 2.6-fold after quartz sand treatment (122, 11.5%), respectively, compared to that of the CER method (23, 4.4%), while low cell lysis (52.1% extracellular component) was obtained based on subcellular localization data. Strong correlations between hydrophobicity of method-specific proteins and β-sheet proportion (r2 = 0.97, p = 0.0024), as well as architecture tightness (r2 = 0.94, p = 0.0069) suggest that once the EPS protein structure becomes loose, exposed inner β-sheet residues are prone to express hydrophobic properties. Addition of surfactants exhibited an undesirable solubilization effect on the protein secondary structure, gel separation, and spatial proteome, irrespective of PN yields. Overall, this work demonstrates the feasibility and effectiveness of extracting anammox EPS using an in situ quartz method that can satisfy yield-lysis trade-offs, retain the natural EPS traits, and allow the collection of more extracellular proteomic information.
[Extract of the Echinacea purpurea herb: an allopathic phytoimmunostimulant]. (1999)

In Northamerican folk medicine Echinacea purpurea L. Moench (purple coneflower) was used as a medicinal plant. Nowadays various formulations containing stabilized or dried pressed juice from Echinacea purpurea as an active ingredient are often administered to treat common colds. These preparations are very well tolerated and safe. Allergic reactions, mainly reversible skin reactions, may occur especially in persons showing hypersensibility after contact with plants from the Compositae family. Pharmacological data let assume purple coneflower pressed juice preparations stimulate the innate immune system and increase the resistance to common colds. In this context the stimulation of the oxidative burst as well as the modulation on monokine secretion by the pressed juice of purple coneflower are reviewed. Also relevant clinical studies are presented concerning the treatment of infections respectively of common cold.
Community Health Services and Blood Lead Reduction in Children (2022)

Objectives: To assess the effect of multidisciplinary community health services on reducing blood lead in children. Material and methods: Retrospective observational study, before-after type. The information of an intervention program in the “Mi Peru” district was analyzed. The population consisted of 1,191 children of both genders who had results of blood lead level and who were evaluated during 2016 and 2017. The sample consisted of 187 children with blood lead levels ≥10 μg/dL. Community health services had three components: comprehensive multidisciplinary care, health education and house calls. Results: The blood lead level at the start of the intervention, 88.5% (n=139) belonged to category II and 11.5% (n=18) belonged to category III. At the end of the intervention, 66.9% (n=105) resulted in category I, the proportion of lead levels belonging to categories II and III was reduced to 29.9% (n=47) and 3.2% (n=5) respectively. In relation to the blood lead level at the beginning of the intervention and at the end of the intervention, the initial mean of 13.96 μg/dL was reduced to 8.96 μg/dL and the median from 12.40 μg/dL to 8.20 μg/dL (p<0.001). Conclusions: Community health services reduced blood lead levels in children with elevated blood lead levels. It is suggested to promote and strengthen the comprehensive house calls and health education about the risks of lead exposure.
PhenoPET: A dedicated PET scanner for plant research based on digital SiPMs (DPCs) (2014)

In the framework of the German Plant Phenotyping Network (DPPN) we developed a novel PET scanner for imaging plants and crops. The observation of the carbon transport within the plant becomes possible by using 11CO2 as PET tracer. The use of the rather short living isotope C-11 asks for a scanner with high dynamic range. That means fast timing and high data rates are important features which let us choose the Philips Digital Photon Counter (DPC) as photo detector. Due to the fast photo detectors and the special crystal matrix arrangement the system will allow measurements with rather high activities. We could measure a coincidence resolution time of ~ 250 ps FWHM between two detector elements. This opens the opportunity to employ time-of-flight information for the first time on a PET scanner of this size. This paper presents very first results from a prototype single-ring system with a FOV of 18 cm diameter and 6.5 cm axial height.
Correlation of Tumor Cytosol Cathepsin D with Differentiation and Invasiveness of Endometrial Adenocarcinoma (1992)

The lysosomal acidic protease cathepsin D, a recognized independent predictor of prognosis in human breast cancer, has not been studied widely in patients with endometrial adenocarcinoma. Cathepsin D levels (52-kD precursor plus 48-kD intermediate and 34/14-kD mature form) were measured in tumor cytosols from 26 hysterectomy specimens by immunoradiometric assay. Significant correlation between cathepsin D levels and tumor differentiation was noted with linear increase in cathepsin D from 8 pmol/mg (standard error of the mean [SEM], 1.73 pmol/mg) for Grade I tumors to 28 pmol/mg (SEM, 3.91 pmol/mg) for Grade III tumors. A group of four papillary serous carcinomas showed relatively high cathepsin D levels reaching 39 pmol/mg. A significant stepwise increase in cathepsin D levels was associated with increased depth of myometrial invasion. Noninvasive tumors averaged 7 pmol/mg (SEM, 4.0 pmol/mg); intramural tumors averaged 15 pmol/mg (SEM, 2.45 pmol/mg); and transmural invasive tumors averaged 30 pmol/mg (SEM, 3.72 pmol/mg). There was no significant correlation of cathepsin D levels with age, estrogen/progesterone receptor hormone status, clinical stage, and lymph node metastasis. Cathepsin D levels correlate significantly with tumor differentiation and myometrial invasiveness and may show promise as a clinically useful adjunct to prognosis assessment and the planning of therapy for patients with endometrial adenocarcinoma.
Electrochemical Investigations of Aromatic Hydrocarbons in Adiponitrile (2015)

Introduction Recent work from this laboratory has shown that adiponitrile (NCCH 2 CH 2 CH 2 CH 2 CN) / tetraethylammonium tetrafluoroborate (TEA BF 4 ) is a versatile solvent system for organic electrochemistry (1,2). The extraordinary 7-Volt potential range noted in earlier work (3) has been confirmed and applied to measurement of water (1) and electrodeposition of metals (4). The +5 V vs Ag/AgCl potential limit allows another 2 volts for study, compared to that of most other solvents. The present paper addresses the usefulness of the positive potential region for oxidation of aromatic hydrocarbons. Experimental Adiponitrile was purchased from Sigma-Aldrich, and tetraethylammonium tetrafluoroborate was purchased from Southwestern Analytical Chemicals. Cyclic voltammograms were obtained on a PAR 283 potentiostat using PowerSuite TM software. Potentials are referenced against a Ag/AgCl reference electrode (1). Results and Discussion A cyclic voltammogram of 10 mM naphthalene in adiponitrile / 0.2 M TEA BF 4 is shown in Figure 1. The reversible reduction of naphthalene is observed at -2.5 V, followed by an irreversible oxidation process at +1.74 V vs Ag/AgCl. Scan reversal at +2.00 V shows no reduction current associated with this oxidation process at 100 mV/s. Continuation of the potential scan to +5.0 V, as shown, results in considerable oxidation current up to the positive potential limit. This charge passage, however, significantly lessens the peak currents for the redox process at -2.5 V on the following sweep, implying some electrode filming in the highly positive potential range. Naphthalene oxidation has also been studied in acetonitrile (5), and the results were similar to those in the present study although the positive scan limit in acetonitrile was only +1.75 V vs SCE. In the acetonitrile study (5), the irreversible nature of naphthalene oxidation was attributed to rapid coupling of naphthalene radical cations. The results of the present study show that the entire positive potential range in adiponitrile is available for study of the representative aromatic hydrocarbon naphthalene. It is also intended to present results for other aromatic systems. References 1. G. T. Cheek, ECS Trans. , 58 (46), 45-51 (2014). 2. G. T. Cheek, ECS Trans. , 61 (30), 19-24 (2014). 3. H. Duncan, N. Salem, and Y. Abu-Lebdeh, J. Electrochem. Soc. , 160 (6), A838-A848. 4. G. T. Cheek, ECS Trans. , 61 (24), 9-14 (2014). 5. M. N. Cortona, N. Vettorazzi, J. J. Silber, and L. Sereno, J. Electroanal. Chem. , 394 , 245-251 (1995). Figure 1. Cyclic voltammogram of 10 mM naphthalene at glassy carbon in adiponitrile / 0.2 M tetraethylammonium tetrafluoroborate. Initial negative-going sweep from 0.00 V vs Ag/AgCl; 100 mV/s Figure 1
The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein. (1988)

We have shown that the minimal enhancer fragment present in the 3'-flanking region of the human beta-globin gene contains four regions that bind nuclear proteins in vitro. By using gel mobility shift and DNase I footprinting assays, we were able to show that each of these regions binds an erythroid-cell-specific nuclear factor which we name NF-E1. This factor is present in erythroid cells at different developmental stages of globin gene expression. The recognition sequence of this protein (A/C Py T/A ATC A/T Py) is also present in the intragenic enhancer and the promoter of the beta-globin gene as well as in the promoter of other erythroid-cell specific genes. In addition to NF-E1, each of the four binding regions interacts with at least one other protein factor.
Characterization of serum matrix metalloproteinase 2/9 levels in patients with ascending aortic aneurysms (2016)

The distinct correlation patterns in patients with and without hyperlipidaemia have to be considered when defining the potential of MMP2 as a biomarker in future studies. The relation between MMP9 and hyperlipidaemia has to be further investigated.
Retinoids and Hox genes (1996)

The vertebrate embryonic body plan is constructed through the interaction of many developmentally regulated genes that supply cells with the essential positional and functional information they require to migrate to their appropriate destination and generate the proper structures. Some molecular cues involved in patterning the central nervous system, particularly in the hindbrain, are interpreted by the Hox homeobox genes. Retinoids can affect the expression of Hox genes in cells lines and embryonic tissues; the hindbrain and branchial region of the head are particularly sensitive to the teratogenic effects of retinoic acid. The presence of endogenous retinoic acid, together with the distribution of retinoid binding proteins and nuclear receptors in the developing embryo, strongly suggest that retinoic acid is a natural morphogen in vertebrate development. The molecular basis for the interaction between retinoic acid and the Hox genes has been aided in part by approaches using deletion analysis in transgenic mice carrying lacZ reporter constructs. Such studies have identified functional retinoic acid response elements within flanking sequences of some of the most 3' Hox genes, suggesting a direct interaction between the genes and retinoic acid. Furthermore, as demonstrated using transgenic mice carrying Hoxb-1/lacZ constructs, multiple retinoic acid response elements may cooperate with positive and negative regulatory enhancers to specify pattern formation in the vertebrate embryo. These types of studies strongly support the normal roles of retinoids in patterning vertebrate embryogenesis through the Hox genes.
The facile fabrication of tunable plasmonic gold nanostructure arrays using microwave plasma (2009)

Fabrication of isolated noble metal nanoparticles embedded in transparent substrates is the fasting growing demand for innovative plasmonic technologies. Here we report a simple and effective methodology for the preparation of highly stable plasmonic nanoparticles embedded in a glass surface. Size-controllable (10-70 nm) Au nanoparticles were rapidly prepared when subjected to the home-microwave plasma. Accordingly, the optical extinction maximum of the localized surface plasmon resonance (LSPR) can be systematically tuned in the range 532-586 nm. We find that the plasmonic structures are exceedingly stable toward immersion in ethanol solvents and pass successfully the adhesive tape test, which makes our system highly promising for efficient transmission-LSPR nanosensors. Besides, the attractive features of substrate-bound plasmonic nanostructures include its low cost, versatility, robustness, reusability and a promising ability to make a multi-arrayed LSPR biochip.
Supplementary Table 2 from Molecular Pathways: The Complexity of the Epigenome in Cancer and Recent Clinical Advances (2023)

&lt;p&gt;PDF file, 66K, Main deregulation of acetylation in cancer. Renown modifications of acetylation in cancer are listed.&lt;/p&gt;
Dynamic Resistance Measurement of a Four-Tape YBCO Stack in a Perpendicular Magnetic Field (2021)

Dynamic resistance occurs when HTS (high-temperature superconductor) coated conductors carry dc current under ac magnetic field. This dissipative effect can play a critical role in many HTS applications. Here, we report on dynamic resistance measurements of a four-tape YBCO stack comprising 4-mm-wide coated conductors, which experience an applied ac perpendicular magnetic field with an amplitude of up to 100 mT. Each tape within the stack carries the same dc current. The magnetic field amplitude, the frequency of the magnetic field, and the dc current magnitude are varied to investigate the influence of these parameters on the dynamic resistance. We find that the threshold field of the stack is significantly larger than that of a single tape when dc current is small, which we attribute to coherent shielding effects from circulating currents present in each wire in the stack. © 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Binding of bovine coagulation factor Xa to platelets (1978)

The binding of highly purified bovine coagulation factor Xa to washed bovine platelets was studied. 125I-labeled factor Xa underwent binding to a platelet receptor that became accessible only after induction of the platelet release reaction by thrombin or by the calcium ionophore A 23187. The zymogen factor X did not bind to platelets. The factor Xa binding was saturable, reversible, and correlated with the rate of thrombin formation. The number of factor Xa binding sites per platelet was 290--420 and the apparent association constant was estimated to be 2.8 x 109 to 1.0 x 1010 M-1. Diisoprophyl fluorophosphate-factor Xa bound to the same platelet receptor as factor Xa indicating that limited proteolysis of a receptor protein was not required for binding. The rate of factor Xa binding was rapid (2.1 x 10(6) to 2.9 x 10(6) M-1 s-1) and similar to that preveiously found for the rate of binding of polypeptide hormones to their receptors. Displacement of factor Xa from the platelet receptor by diisopropyl fluorophosphate-factor Xa effectively blocked thrombin formation. Low concentrations of factor Xa catalyze prothrombin activation more effectively in the presence of platelets than in the presence of phospholipid and factor V.
Thermogravimetric behavior of starches (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThermogravimetric behavior of starchesBenjamin. Carroll and John W. LiskowitzCite this: J. Agric. Food Chem. 1967, 15, 4, 701–703Publication Date (Print):July 1, 1967Publication History Published online1 May 2002Published inissue 1 July 1967https://doi.org/10.1021/jf60152a025RIGHTS & PERMISSIONSArticle Views131Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (277 KB) Get e-Alerts Get e-Alerts
PD-1-Dependent Mechanisms Maintain Peripheral Tolerance of Donor-Reactive CD8+ T Cells to Transplanted Tissue (2008)

Peripheral mechanisms of self-tolerance often depend on the quiescent state of the immune system. To what degree such mechanisms can be engaged in the enhancement of allograft survival is unclear. To examine the role of the PD-1 pathway in the maintenance of graft survival following blockade of costimulatory pathways, we used a single-Ag mismatch model of graft rejection where we could track the donor-specific cells as they developed endogenously and emerged from the thymus. We found that graft-specific T cells arising under physiologic developmental conditions at low frequency were actively deleted at the time of transplantation under combined CD28/CD40L blockade. However, this deletion was incomplete, and donor-specific cells that failed to undergo deletion up-regulated expression of PD-1. Furthermore, blockade of PD-1 signaling on these cells via in vivo treatment with anti-PD-1 mAb resulted in rapid expansion of donor-specific T cells and graft loss. These results suggest that the PD-1 pathway was engaged in the continued regulation of the low-frequency graft-specific immune response and thus in maintenance of graft survival.
A Qualitative Study to Understand Barriers to Implementation of National Guidelines for Prehospital Termination of Unsuccessful Resuscitation Efforts (2010)

We have identified operational barriers that may impede implementation of TOR guidelines. Three influential stakeholder groups will need to work with national organizations to overcome these local barriers.
A Study of Maximum Voluntary Strength Evaluation for Korean Workers (1992)

In order to evaluate task loads required by a majority of manual materials handling(MMH) jobs, we have performed the assessment of arm strength, leg strength and torso strength for Korean workers in a scientific and systematic manner. Two hundred and eighty five employees from two local manufacturing plants in Pohang participated in this project. Maximum voluntary strengths were assessed for the aforementioned three postures, and these data were classified by gender. These strength data were also used to generate a strength predicition model, which can estimate the maximum voluntary strength for a population with specific age, height and weight. These strength data will be used for the prevention of occupational injuries which are frequently incurred by overexertion in manual materials handling jobs.
Development of new material systems for direct laser freeform fabrication (2006)

Laser freeform fabrication comprises selective laser sintering and laser melting technologies and allows the production of complex 3D-workpieces by solidifying powder materials like polymers, metals, ceramics and composites. The development of new material systems for the laser generating process requires time and cost intensive experiments. Final properties of laser generated layers and parts depend on process parameters as well as on characteristics of powder materials. With this contribution, a method is presented to reduce experimental studies by a theoretical analysis of the process. With the presented simulation, the influence of processing parameters can be derived and identified for a big variety of materials. The developed model has been validated on selected metals and metal ceramic composites. The combination of theoretical calculation of material behaviour and experiments, with respect to the type of laser, reduces the effort for process parameter optimization significantly. Based on this method there has been developed a new metal ceramic composite in order to satisfy tribological applications for components in fluid machines.
Angiography with metrizamide. Animal experiments and preliminary clinical experiences. (1977)

The non-ionic contrast medium metrizamide (Amipaque) was compared in animal experiments with diatrizoate, iothalamate and metrizoate. Vasodilatory effects, acute intravenous toxicity and effects on red blood cells, pulmonary arterial pressure, endothelium and heart were all less after metrizamide than after the ionic media. This motivated clinical trial of metrizamide for angiography, which demonstrated that metrizamide produced less pain than the ionic monomeric media in femoral as well as in other types of angiography.
Genetics of hypertrophic cardiomyopathy: one, two, or more diseases? (2007)

Genomic medicine has entered clinical practice and the diagnostic utility of genetic testing for hypertrophic cardiomyopathy is clearly evident, but with the growing number of hypertrophic cardiomyopathy-associated genes strategic choices have to be made. With recent discoveries in genotype-phenotype relationships, especially pertaining to the echocardiographic septal shape and the underlying pathogenetic mutation, time has come to subdivide the one disease we call hypertrophic cardiomyopathy.