gebhart/patrepeval huggingface dataset:
AsopBDeletion Mutation Enhances the Immunogenicity and Protective Efficacy of a Heterologous Antigen Delivered by Live AttenuatedSalmonella entericaVaccines (2008)

SopB is a virulence factor of Salmonella encoded by SPI-5. Salmonella sopB deletion mutants are impaired in their ability to cause local inflammatory responses and fluid secretion into the intestinal lumen and also can enhance the immunogenicity of a vectored antigen. In this study, we evaluated the effects on immunogenicity and the efficacy of a sopB deletion mutation on two Salmonella enterica serovar Typhimurium vaccine strains with different attenuating mutations expressing a highly antigenic alpha-helical region of the Streptococcus pneumoniae surface protein PspA from an Asd(+)-balanced lethal plasmid. After oral administration to mice, the two pairs of strains induced high levels of serum antibodies specific for PspA as well as to Salmonella antigens. The levels of antigen-specific serum immunoglobulin G (IgG) and mucosal IgA were higher in mice immunized with sopB mutants. Enzyme-linked immunospot assay results indicated that the spleen cells from mice immunized with a sopB mutant showed higher interleukin-4 and gamma interferon secretion levels than did the mice immunized with the isogenic sopB(+) strain. The sopB mutants also induced higher numbers of CD4(+) CD44(hi) CD62L(hi) and CD8(+) CD44(hi) CD62L(hi) central memory T cells. Eight weeks after primary oral immunization, mice were challenged with 100 50% lethal doses of virulent S. pneumoniae WU2. Immunization with either of the sopB mutant strains led to increased levels of protection compared to that with the isogenic sopB(+) parent. Together, these results demonstrate that the deletion of sopB leads to an overall enhancement of the immunogenicity and efficacy of recombinant attenuated Salmonella vaccine strains.
Implementation Scheme and Performance Analysis of RLC in TD-SCDMA (2006)

Radio link control (RLC) is one part of the layer 2, in TD-SCDMA. This paper proposes a new and efficient scheme for the implementation of RLC, and analyzes the relevant performance. One model about SDU delay time for RLC process, is proposed and used to analyze the relevant performance. Through our implementation scheme and this SDU delay model, some RLC protocol parameters, including MaxDAT in No_discard mode, window size, are analyzed, so as to provide some reference to configure RLC protocol parameters, for the particular type of service in TD-SCDMA system
Food (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFoodArthur K. Foltz, James A. Yeransian, and Katherine G. SlomanCite this: Anal. Chem. 1983, 55, 5, 164–196Publication Date (Print):April 1, 1983Publication History Published online1 May 2002Published inissue 1 April 1983https://pubs.acs.org/doi/10.1021/ac00256a012https://doi.org/10.1021/ac00256a012research-articleACS PublicationsRequest reuse permissionsArticle Views142Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Micrometer-Sized Mesoporous Silica Spheres Grown under Static Conditions (1998)

Mesoporous silica spheres ranging from 2 to 6 μm in size were synthesized under static acidic conditions by mixed cationic−nonionic surfactant templating. The mesoporous structure of the calcined silica microspheres was characterized by X-ray diffraction (XRD) and nitrogen sorption, whereas the morphology and size of the microspheres were revealed by scanning electron microscopy (SEM). It was shown that stirring is unfavorable for the formation of silica spheres in the present synthesis. The growth of the micrometer-sized mesoporous silica spheres was examined by transmission electron microscopy (TEM), and an undirectional aggregation process was proposed for the formation of these spheres.
Importance of Prolonged Compression during Cardiopulmonary Resuscitation in Man (1977)

Standards for external cardiac compression include compression of the chest for 50 per cent of cycle time and a compression rate of 60 per minute.1 Warnings are given against "quick jabs." Animal studies do, in fact, suggest that the duration of prolonged compression is important in effective resuscitation.2 To achieve a duration of 50 per cent during manual external cardiac compression, there must be a distinct pause at maximal compression. Application of this principle to man appears to have been slow, perhaps because of doubts about the applicability of animal data to man. In view of the obvious importance of . . .
Between regions and objects: surfaces and volumes (1977)

Several modular processes are used to build relationships between a) two-dimensional regions and boundaries in a segmented image, and b) the hypothesized surfaces, volumes and objects of a three-dimensional world. A variety of bottom-up and top-down strategies coordinate the processes of SHAPE, PERSPECTIVE, SHADOW, and OCCLUSION to construct these relationships in the VISIONS system.
Spiral Galaxy Rotation Curves in the Brane World Theory in Five Dimensions (2008)

Equations of motion, in cylindrical coordinates, for the observed rotation of gases within the gravitational potential of spiral galaxies are derived from Carmeli's 5-Dimensional Cosmological General Relativity theory. A Tully-Fisher type relation results, and rotation curves are reproduced without the need for non-baryonic halo dark matter. Two acceleration regimes are found that are separated by a critical acceleration ≈ 4.75 X 10⁻¹⁰ m/s².For accelerations larger than the critical value the Newtonian force law applies, but for accelerations less than the critical value the Carmelian regime applies. In the Newtonian regime the accelerations fall off as r⁻², but in the Carmelian regime the accelerations fall off as r⁻¹. This is new physics, but it fits Milgrom's phenomenological MOND theory. The following is based on the work of Hartnett (Hartnett 2006).
A Comparison of High-Latitude Ionosphere Propagation Predictions from AMBCOM with Measured Data (1991)

This thesis examines the performance of SRI's Ambient Communications (AMBCOM) model for high latitude propagation prediction. It is one in a series of studies, conducted at the Naval Postgraduate School, to establish the relative merits of several computer-based propagation prediction models using a standard set of measured data. AMBCOM modeled the propagation path between a transmitter located in the polar cap region and several midlatitude receiver sites. Model predictions were matched to measured data obtained during two high- latitude communication experiments (campaigns). The absolute difference between model signal-to-noise ratio (SNR) and measured SNR was considered as error. Error statistics were accumulated to show the distribution of the error by campaign and frequency. The percentage, by frequency, of matched AMBCOM predictions in reference to total predictions for a given frequency was considered a measure of AMBCOM performance. AMBCOM exhibited small absolute values of average error, i.e., 7-11 dB, and high percentages of matched records. The average error was typically distributed between -20 and +20 dB. Unfortunately, these are only relative measures of model performance. The site antenna and environmental data used to model high latitude campaigns were estimated not measured, and some variation in AMBCOM results may be attributable to poor estimates. The measured data were not designed specifically for model validation, and further comparisons are needed with new measured data.
Isolation and characterization of actin from mackerel dark muscle. (1983)

G-Actin was extracted from the acetone powder of the dark muscle of mackerel Pneumatophorys japonicus japonicus, and purified by a method which essentially consisted of polymerization in 0.1 M KCl containing 0.1 mM MgCl2, treatment with 0.6 M KCl, and Sephadex G-200gel filtration. Sedimentation constant (s020, W) of the dark muscle actin was 2.9S. The molecular weight was determined to be 42, 000by SDS-gel electrophoresis, The intrinsic viscosity [η] was 5.3 dl/g in F-form. It featured higher glutamic acid, aspartic acid and alanine contents, and lower histidine and tryptophan contents. One mole of 3-methylhistidine per mole actin was detected. The above physicochemical properties were essentially the same as those of actins from various sources, including mackerel ordinary muscle.
Effects of stress on the exfoliation of polystyrene nanocomposites (2004)

Polystyrene/organoclay nanocomposites have been prepared by melt blending in a vertical co-rotating twin-screw mixer. Monodisperse polymers having molecular weights of 18k and 49k were investigated. Low molecular weight polystyrenes were chosen to take advantage of the high viscosities near Tg, allowing temperature variation to provide for several orders of magnitude of viscosity and to correspondingly change the shear stress. Melt rheology was the primary tool used to determine the extent of exfoliation in the nanocomposite samples. The highest amount of exfoliation at low clay loading was present in samples with an 18k matrix favoring low temperature. A bimodal polystyrene matrix facilitated dispersion, but the low molecular weight chains compromised the final moduli.
A convenient procedure for the monosilylation of symmetric 1,n-diols (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA convenient procedure for the monosilylation of symmetric 1,n-diolsPatrick G. McDougal, Joseph G. Rico, Young Im Oh, and Brian D. CondonCite this: J. Org. Chem. 1986, 51, 17, 3388–3390Publication Date (Print):August 1, 1986Publication History Published online1 May 2002Published inissue 1 August 1986https://pubs.acs.org/doi/10.1021/jo00367a033https://doi.org/10.1021/jo00367a033research-articleACS PublicationsRequest reuse permissionsArticle Views8213Altmetric-Citations204LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
An integrated design environment of fault tolerant processors with flexible HW/SW solutions for versatile performance/cost/coverage tradeoffs (2017)

This paper presents an integrated design environment (IDE) for embedded fault-tolerant processor system. It takes in a processor core IP and the embedded software which is to be executed on the given processor, and turns them into a fault-tolerant system with various hardware and software mechanisms, subject to the designer's selection. The hardware options include dual redundancy for processor core, and single-error-detection/correction protections for memory. The software option is control flow error detection. A GUI is provided for the designer to select the options and the IDE automatically generates the hardware Verilog code and the modified embedded software. The IDE reports the cost, speed and power consumption of the generated hardware and the static and dynamic instruction counts of the generated software. In addition, a fault-injection tool is also provided to evaluate the fault coverage of the generated hardware and software. With this IDE, the designer could explore the tradeoffs of cost/performance/ power/fault-coverage. We have successfully demonstrated this IDE with an industrial processor core Andes N8.
Revised Sequence and Expression of Cyclin B cDNA from the Starfish Asterina pectinifera (2001)

Cyclin B cDNA was cloned from the ovary of the starfish Asterina pectinifera and analyzed by RT-PCR and 3'- and 5'-RACE techniques. The cDNA consists of a 0.13-kb upstream untranslated region, a 1.22-kb coding region, and a 0.86-kb downstream untranslated region. The open reading frame encoded a polypeptide of 404 amino acid residues with a calculated molecular weight of 45,692. All the characteristic sequences, such as destruction and cyclin boxes, cyclin B motif, and cytoplasmic retention and nuclear export signals, were found in the newly cloned cyclin B cDNA. The deduced amino acid sequence of the cyclin B cDNA was highly homologous in the middle and carboxy terminal regions to that from mature eggs of the same organism, but quite different in the amino terminal region. Evidence was obtained which suggested that this cyclin B is expressed in immature and maturing oocytes and is the same as that cloned from mature eggs.
Regulation of financial systems and economic growth in OECD countries (2007)

The operation of the financial system can have a key impact on economic growth and the stability of the economy. It affects long-term economic growth through its effect on the efficiency of intermediation between the savers and final borrowers of funds; through the extent to which it allows for monitoring of the users of external funds, affecting thereby the productivity of capital employed; and through its implications for the volume of saving, which influences the future income-generating capacity of the economy. It affects the stability of the economy because of the high degree of leverage of its activities and its pivotal role in the settlement of all transactions in the economy, so that any failure in one segment risks undermining the stability of the whole system.
Glutaraldehyde Cross-Linked Glutamate Oxidase Coated Microelectrode Arrays: Selectivity and Resting Levels of Glutamate in the CNS (2013)

Glutaraldehyde is widely used as a cross-linking agent for enzyme immobilization onto microelectrodes. Recent studies and prior reports indicate changes in enzyme activity and selectivity with certain glutaraldehyde cross-linking procedures that may jeopardize the performance of microelectrode recordings and lead to falsely elevated responses in biological systems. In this study, the sensitivity of glutaraldehyde cross-linked glutamate oxidase-based microelectrode arrays to 22 amino acids was tested and compared to glutamate. As expected, responses to electroactive amino acids (Cys, Tyr, Trp) were detected at both nonenzyme-coated and enzyme-coated microelectrodes sites, while the remaining amino acids yielded no detectable responses. Electroactive amino acids were effectively blocked with a m-phenylene diamine (mPD) layer and, subsequently, no responses were detected. Preliminary results on the use of poly(ethylene glycol) diglycidyl ether (PEGDE) as a potentially more reliable cross-linking agent for the immobilization of glutamate oxidase onto ceramic-based microelectrode arrays are reported and show no significant advantages over glutaraldehyde as we observe comparable selectivities and responses. These results support that glutaraldehyde-cross-linked glutamate oxidase retains sufficient enzyme specificity for accurate in vivo brain measures of tonic and phasic glutamate levels when immobilized using specific "wet" coating procedures.
SU‐E‐J‐107: Supervised Learning Model of Aligned Collagen for Human Breast Carcinoma Prognosis (2014)

Purpose: Our goal is to develop and apply a set of optical and computational tools to enable large‐scale investigations of the interaction between collagen and tumor cells. Methods: We have built a novel imaging system for automating the capture of whole‐slide second harmonic generation (SHG) images of collagen in registry with bright field (BF) images of hematoxylin and eosin stained tissue. To analyze our images, we have integrated a suite of supervised learning tools that semi‐automatically model and score collagen interactions with tumor cells via a variety of metrics, a method we call Electronic Tumor Associated Collagen Signatures (eTACS). This group of tools first segments regions of epithelial cells and collagen fibers from BF and SHG images respectively. We then associate fibers with groups of epithelial cells and finally compute features based on the angle of interaction and density of the collagen surrounding the epithelial cell clusters. These features are then processed with a support vector machine to separate cancer patients into high and low risk groups. Results: We validated our model by showing that eTACS produces classifications that have statistically significant correlation with manual classifications. In addition, our system generated classification scores that accurately predicted breast cancer patient survival in a cohort of 196 patients. Feature rank analysis revealed that TACS positive fibers are more well aligned with each other, generally lower density, and terminate within or near groups of epithelial cells. Conclusion: We are working to apply our model to predict survival in larger cohorts of breast cancer patients with a diversity of breast cancer types, predict response to treatments such as COX2 inhibitors, and to study collagen architecture changes in other cancer types. In the future, our system may be used to provide metastatic potential information to cancer patients to augment existing clinical assays.
Effects of mimic-miR-720 on stem cell phenotype of DPCs. (2015)

&lt;p&gt;A) Quantitative assessment of miR-720 levels. *** P&lt;0.001, One way ANOVA/Tukey. Results are representative of at least 3 independent experiments. B) mimic-miR-720 reduced mRNA levels of &lt;i&gt;NANOG&lt;/i&gt;, but increased the levels of &lt;i&gt;DNMT3B&lt;/i&gt; and &lt;i&gt;DNMT1&lt;/i&gt; mRNA. No significant changes were observed in &lt;i&gt;DNMT3A&lt;/i&gt; mRNA upon miR-720 transfection. * P&lt;0.05, ** P&lt;0.01, NS = non-significant, unpaired &lt;i&gt;t&lt;/i&gt;-test, compared to si-control group. Results are representative of at least 3 independent experiments. C) Representative images of immunocytochemistry for NANOG protein levels. D) Quantitative analysis of NANOG or DNMTs positive cells. Mimic-miR-720 significantly reduced the number of NANOG positive cells, while increasing those for DNMT3B. There was no significant change in the percentages of DNMT1 or DNMT3A positive cells. Results in C–D are representative of at least 3 independent experiments. Quantitative analysis was performed on 500 cells/well, with at least triplicate samples. * P&lt;0.05, ** P&lt;0.01, NS = non-significant, unpaired &lt;i&gt;t&lt;/i&gt;-test, compared to si-control group. G) Transfection of mimic-miR-720 decreased the percentage of SSEA-4&lt;sup&gt;+&lt;/sup&gt; cells. Results are representative of 3 independent experiments.&lt;/p&gt;
Biomedical engineering and bioscience students address hypoxia‐related research problems in an interdisciplinary physiology laboratory course (2008)

Innovative transformational research is being promoted by the NSF Strategic Plan 2006–2011, with multidisciplinary projects emphasized. To prepare undergraduates for careers that cross disciplinary boundaries, bioscience students need to practice interdisciplinary communication in academic programs that connect students in diverse disciplines. This report describes a physiology laboratory course being piloted to create and study this educational frontier. Measurement and Design Issues for Experimental Physiology is a course where biomedical engineering and biology students apply knowledge from both fields while cooperating in multidisciplinary teams under specified technical constraints. Students use information, tools, techniques, and theories from their disciplines to solve problems that are beyond the scope of any single discipline. Instructional guidelines are provided to promote effective communication, systems thinking, laboratory skills, self‐management, and professional ethics needed for hypoxia‐related research. This project recognized different problem‐solving approaches used by students of engineering and bioscience, broke down stereotypes between the disciplines, and developed an educational environment where interdisciplinary teamwork was used to bridge differences.
Identification of a Yeast Peroxisomal Member of the Family of AMP‐Binding Proteins (1996)

We established a reverse-genetic approach to identify peroxisomal proteins involved in peroxisomal fatty acid metabolism of Saccharomyces cerevisiae. Putative peroxisomal peripheral membrane proteins were isolated by successive extraction of purified peroxisomes and purified by HPLC and SDS/PAGE. Six proteins were identified by peptide sequence analysis, including acyl-CoA oxidase and a trifunctional enzyme of the peroxisomal beta-oxidation system as well as peroxisomal malate dehydrogenase 3 and carnitine acetyltransferase. In addition two previously unknown putative peroxisomal proteins were identified, an unknown 40-kDa protein and a protein which we named Pcs60p, both major constituent of the isolated protein fraction. Pcs60p is encoded by ORF Z36091 of chromosome II from S. cerevisiae and consists of 543 amino acids with a molecular mass of 60.5 kDa. Biochemical, immunofluorescence microscopy and immunocytochemical data confirmed that Pcs60p is a peroxisomal peripheral membrane protein but the protein is also localized in the peroxisomal matrix. Consistent with the intraperoxisomal localization, the consensus sequence for a peroxisomal-targeting signal 1 (PTS1) is present at the extreme C-terminus of Pcs60p. Deletion studies revealed that the peroxisomal localization of the protein depends on the presence of this signal sequence. Expression of Pcs60p is highly inducible by oleic acid, however, the protein is dispensable for growth on oleic acid as single carbon source. Pcs60p belongs to the family of proteins which act via an ATP-dependent covalent binding of AMP to their substrates and shows the highest degree of similarity to the Escherichia coli long chain acyl-CoA synthetase.
A compact planar gradiometer system for measuring tangential components of biomagnetic fields (1997)

Presents a compact and reliable planar gradiometer for a multichannel system measuring tangential components of biomagnetic fields. The readout electronics was simplified by using a double relaxation oscillation SQUID (DROS) which provides very large flux-to-voltage transfer of typically 3 mV//spl Phi//sub 0/, enabling direct readout by a DC preamplifier with modest voltage noise. The DROS was made from hysteretic Nb/AlO/sub x//Nb junctions. To realize a compact and reliable gradiometer, the planar pickup coil is integrated on the same wafer with the SQUID. The planar pickup coil consists of two 10 mm/spl times/10 mm coils connected in series to form a first-order gradiometer and has a baseline of 30 mm. The field gradient noise is 9 fT/cm/spl radic/Hz at 1 Hz inside a magnetically shielded room. The gradiometers were arranged to measure simultaneously x and y components of biomagnetic fields.