gebhart/patrepeval huggingface dataset:
Naturally Occurring Mutations in Glycoprotein Ibα That Result in Defective Ligand Binding and Synthesis of a Truncated Protein (1998)

The platelet GPIb-V-IX complex is the receptor for the initial binding of von Willebrand factor (vWF) mediating platelet adhesion. The complex is composed of four membrane-spanning glycoproteins (GP): GPIbalpha, GPIbbeta, GPIX, and GPV. Bernard-Soulier syndrome results from a qualitative or quantitative defect in one or more components of the platelet membrane GPIb-V-IX complex. We describe the molecular basis of a novel Bernard-Soulier syndrome variant in two siblings in whom GPIbalpha was not detected on the platelet surface but that was present in a soluble form in plasma. DNA sequence analysis showed that the affected individuals were compound heterozygotes for two mutations. One, inherited from a maternal allele, a T777 --> C point mutation in GPIbalpha converting Cys65 --> Arg within the second leucine rich repeat, the other, a single nucleotide substitution (G2078 --> A) for the tryptophan codon (TGG) causing a nonsense codon (TGA) at residue 498 within the transmembrane region of GPIbalpha, inherited from a mutant paternal allele. The Bernard-Soulier phenotype was observed in siblings who were compound heterozygotes for these two mutations. Although GPIbalpha was not detected on the surface of the patient's platelets, soluble GPIbalpha could be immunoprecipitated from plasma. When plasmids encoding GPIbalpha containing the Cys65 --> Arg mutation were transiently transfected into Chinese hamster ovary (CHO) cells stably expressing the GPbeta-IX complex (CHObetaIX), the expression of GPIbalpha was similar to the wild-type (WT) GPIbalpha, but did not bind vWF. When plasmids encoding GPIbalpha containing the Trp498 --> stop were transiently transfected into CHObetaIX, the surface expression of GPIbalpha was barely detectable compared with the WT GPIbalpha. Thus, this newly described compound heterozygous defect produces Bernard-Soulier syndrome by a combination of synthesis of a nonfunctional protein and of a truncated protein that fails to insert into the platelet membrane and is found circulating in plasma.
Technique of temporary subcutaneous “Figure‐of‐eight” sutures to achieve hemostasis after removal of large‐caliber femoral venous sheaths (2011)

Over the last decade, significant advances have been made in percutaneous treatment of structural heart diseases. Many of these interventions require insertion of large caliber sheaths in the femoral veins. Manual compression, compression devices, and various closure devices have been used for removal of large-sized venous sheaths. Here, we describe the use of a temporary subcutaneous "Figure-of-Eight" suture technique for venous access site closure after removal of large-caliber sheaths.
Dominant negative inhibition by mutant thyroid hormone receptors is thyroid hormone response element and receptor isoform specific. (1993)

The heterogeneity of tissue-specific manifestations of generalized resistance to thyroid hormone (GRTH) could result from differential interactions between the mutant thyroid hormone (T3) receptor-beta (TR beta) on T3 response elements (TREs) in different T3-responsive genes. To explore this hypothesis, the mutant TR beta associated with kindred A, P448H; a TR beta mutant, P448L; and a comparable TR alpha mutant (P398H) were tested for intrinsic function and for inhibition of wild-type TR alpha- and -beta-induced expression from four structurally distinct TREs, the rGH ABC*, the rGH palindrome (PAL), the rat malic enzyme (ME), and the chicken lysozyme silencer F2 (F2). The relative function of the mutants was similarly reduced on the four TREs studied and was T3 concentration dependent. The TR alpha mutant retained the intrinsically greater potency characteristic of this isoform, but remained impaired with respect to wild-type TR alpha even at 500 nM T3. In general, dominant negative inhibition of wild-type TR alpha and -beta function was dependent upon the T3 concentration, as expected from the decreased affinity for ligand conferred by this mutation. A T3 concentration sufficient to relieve the inhibition of wild-type TR function on the ABC*, PAL, and ME TREs (50 nM) had no effect on inhibition of the F2 TRE by the mutant TRs. Receptor isoform preferential inhibition was observed on the ABC*, PAL, and ME TREs by the mutant TRs. Thus, both TRE structure and the isoform of endogenously active receptor could determine the degree of inhibition of a specific gene in GRTH individuals. Further, the lack of dominant negative potentials does not explain the absence of TR alpha mutations in GRTH kindreds.
Liver Tissue Engineering Utilizing Hepatocytes Propagated in Mouse Livers in Vivo (2012)

Recent advances in tissue engineering technologies have highlighted the ability to create functional liver systems using isolated hepatocytes in vivo. Considering the serious shortage of donor livers that can be used for hepatocyte isolation, it has remained imperative to establish a hepatocyte propagation protocol to provide highly efficient cell recovery allowing for subsequent tissue engineering procedures. Donor primary hepatocytes were isolated from human α-1 antitrypsin (hA1AT) transgenic mice and were transplanted into the recipient liver of urokinase-type plasminogen activator-severe combined immunodeficiency (uPA/SCID) mice. Transplanted donor hepatocytes actively proliferated within the recipient liver of the uPA/SCID mice. At week 8 or later, full repopulation of the uPA/SCID livers with the transplanted hA1AT hepatocytes were confirmed by blood examination and histological assessment. Proliferated hA1AT hepatocytes were recovered from the recipient uPA/SCID mice, and we generated hepatocyte sheets using these recovered hepatocytes for subsequent transplantation into the subcutaneous space of mice. Stable persistency of the subcutaneously engineered liver tissues was confirmed for up to 90 days, which was the length of our present study. These new data demonstrate the feasibility in propagating murine hepatocytes prior to the development of hepatic cells and bioengineered liver systems. The ability to regenerate and expand hepatocytes has potential clinical value whereby procurement of small amounts of tissue could be expanded to sufficient quantities prior to their use in hepatocyte transplantation or other hepatocyte-based therapies.
Hollywood: dalla specializzazione alla produzione globale (2005)

The paper discusses the application of the Italian model of industrial and cultural districts to the movie industry based in Hollywood. Such a complex production has passed through a long evolution in which we can identify three stages: a first period characterised by mass production of movies and fordist organisation of production; a second period, much closer to the Italian districtual model, defined by the «flexible specialisation», induced by the mass diffusion of television and the related need to emphasize the identity of each single movie; a third period in which globalisation and outsourcing appear the crucial aspects of the cinema industry. Within such a model, whose distance from the districtual model is progressively growing, communication investments are a strong component of the whole budget, and the progressive decrease of critical elements in the artistic side of production is the most evident limit.
Cantilever-free thermal actuation (2013)

The authors report the characterization and optimization of thermal actuators based upon thin elastomeric films on glass slides with integrated microheaters. By performing a systematic study of actuation performance with respect to heater size and elastomeric film thickness, the relationships between these parameters and actuation speed, efficiency, and crosstalk are elucidated. Combining these experimental studies with calculated temperature profiles provides an estimate of the maximum attainable actuation, which is predicted to be as large as 20% of the elastomeric film thickness. Based on these results, the authors provide a strategy for optimizing actuator geometry for a desired application in terms of selected actuation range and temperature tolerance. These results can be used to explore the feasibility of applying thermal actuation in a massively parallel format in low-cost microelectromechanical systems for applications such as high throughput, individually addressable cantilever-free scanning probe lithography.
Analysis of lecithin in cosmetics by reversed‐phase liquid chromatography/electrospray tandem mass spectrometry (1998)

A rapid, sensitive and selective method by reversed-phase liquid chromatography (LC) with electrospray ionization (ESI) mass spectrometry (MS) was employed for the determination of lecithin in a cosmetic raw material for quality control of the product formulation. The mixture of the phosphatidylcholine, phosphatidyl-ethanolamine, and phosphatidylinositol in lecithin was separated using aqueous methanol–acetonitrile solvent with a reversed-phase LC column, and detected as forms of pseudomolecular ions. Using this LC/ESI-MS technique, simultaneous separation and detection of phospholipid classes and molecular species within each class were achieved for the first time. Additionally informative fragmentation patterns were obtained by employing LC/ESI-MS/MS in both positive and negative ionization modes to identify these fatty acid chains and polar head groups in each molecular species of the phospholipids. © 1998 John Wiley & Sons, Ltd.
Editorial (2015)

Durch neue biomechanische Erkenntnisse, neue Materialien und Implantate sowie Fortschritte in der Plastischen Chirurgie besonders auf dem Gebiet der Mikrochirurgie haben sich die Rekonstruktionsmöglichkeiten bei Handverletzungen in den letzten 20 Jahren stark erweitert. Auch die präventiven Maßnahmen zur Vermeidung von Unfällen haben besonders bei den Arbeitsunfällen erfolgreiche Senkungen der Zahlen von schweren Handverletzungen bewirken können. Dabei soll nicht verschwiegen werden, dass es nach wie vor noch viele ungelöste Probleme besonders in der Gelenkchirurgie an der Hand gibt.
Modulation of IL‐4‐induced human IgE production in vitro by IFN‐γ and IL‐5: The role of soluble CD23 (s‐CD23) (1989)

IL-4 specifically induced IgE production by peripheral blood lymphocytes or by tonsil or spleen cells from healthy donors. IL-4-induced IgE synthesis was dependent on CD4+ T cells and monocytes and was blocked by IFN-gamma, IFN-alpha, and prostaglandin E-2 (PGE-2). These substances also inhibited IL-4-induced CD23 expression and subsequent release of soluble CD23 (s-CD23). In addition, IgE production was blocked by F(ab')2 fragments of an mAb against CD23. In contrast, IL-5 enhanced IL-4-induced IgE production, provided IL-4 was added at nonsaturating concentrations. This increase in IgE production correlated quantitatively with an enhanced release of s-CD23. Collectively, these results indicate that there is a correlation between s-CD23 release and IgE production. However, s-CD23 fractionated from supernatants of the lymphoblastoid cell line RPMI-8866 was ineffective in inducing IgE production in the absence of IL-4, but acted synergistically with suboptimal concentrations of IL-4. In addition, it is demonstrated that alloreactive T-cell clones produced varying concentrations of IL-4, IL-2, or IFN-gamma upon stimulation. Only supernatants of 2/4 of these T-cell clones induced a low degree of IgE synthesis, but in the presence of anti-IFN-gamma antibodies, all four supernatants induced a strong induction of IgE production. This IgE synthesis was blocked specifically by anti-IL-4 antibodies, indicating that IL-4 is the sole inducer of IgE synthesis. Our findings demonstrate that IL-4-induced IgE production involves complex interactions of T cells, B cells, and monocytes and is positively modulated by IL-5 and s-CD23 but down-regulated by IFN-gamma, IFN-alpha, and PGE-2, respectively.
Guidance with real-time CT fluoroscopy: early clinical experience. (1996)

A recently developed real-time computed tomography (CT) fluoroscopy system, which provides effective real-time reconstruction and display of CT images, was used to monitor nonvascular interventional procedures performed in 57 patients. Biopsy of thoracic lesions (n = 38), biopsy or drainage of pelvic lesions (n = 6), drainage or aspiration of intracranial hematomas (n = 9), and other procedures (n = 4) were performed. CT fluoroscopy successfully depicted the entire procedure in all patients. In thoracic lesions, a mean 1.3 passes was necessary to gain access to the lesion. Sufficient cytologic samples were obtained in 32 of 33 pulmonary lesions with a mean diameter of 26 mm.
Low-temperature deposition of Y-Ba-Cu-O films on a CaF2/GaAs substrate (1989)

Superconducting thin films of Y-Ba-Cu-O were fabricated on a CaF2/GaAs(100) substrate using the rf planar magnetron sputtering technique. Substrates were heated as low as 450 °C during deposition. The as-deposited films without high-temperature post-annealing have a transition temperature of 75 K and zero resistance temperature of 45 K. The perovskite structure usually observed in this system was not observed. The critical current (Jc) value of 2×103 A/cm2 was measured at 4.2 K.
The charging level of a ligament-droplet system atomized in a uniform electric field (2014)

In this paper, a numerical algorithm has been formulated for calculating the charge magnitude on a spherical droplet created by break-up of a cylindrical ligament in an external electric field. The algorithm is based on the Finite Element Method and it determines the droplet charge as a function of the droplet radius and ligament length. It has been found that the droplet charge is a function of particle radius to some exponent, which is equal to 2 when the droplet is in direct contact with the atomizer and decreases dramatically to approach 1.1, as the ligament length increases. The effect of the ligament radius on the charging level has been found to be significant and increases with the ligament length. It was determined that the charging level is affected by the presence of adjacent ligaments and previously ejected droplets. To investigate the dynamics of the droplet atomization, the Navier-Stokes equations, describing the fluid motion, as well as the level-set equation for tracking the interfaces between the air and liquid have been solved. A comparison between the charging level of the dynamically formed droplets and an assumed spherical one has been presented. It has been found that the predicted charging level of the spherical droplet is lower than the one of the actual shape in a range of 15-26%.
Scrambled digital signals (2001)

While digital television broadcasting has been one of the hottest stories in Australian media over the past few years, that other kind of broadcasting---radio---has been decidedly cool about any transition to digital. [Introduction]
Intrinsic Dimension Estimation by Maximum Likelihood in Probabilistic PCA (2013)

International audience
Equinatoxin II Potentiates Temozolomide- and Etoposide-Induced Glioblastoma Cell Death (2012)

Glioblastoma (GBM) is considered incurable due to its resistance to current cancer treatments. So far, all clinically available alternatives for treating GBM are limited, evoking the development of novel treatment strategies that can more effectively manage these tumors. Extensive effort is being dedicated to characterize the molecular basis of GBM resistance to chemotherapy and to explore novel therapeutic procedures that may improve overall survival. Cytolysins are toxins that form pores in target cell membranes, modifying ion homeostasis and leading to cell death. These pore-forming toxins might be used, therefore, to enhance the efficiency of conventional chemotherapeutic drugs, facilitating their entrance into the cell. In this study, we show that a non-cytotoxic concentration of equinatoxin II (EqTx-II), a pore-forming toxin from the sea anemone Actinia equina, potentiates the cytotoxicity induced by temozolomide (TMZ), a first-line GBM treatment, and by etoposide (VP-16), a second- or third-line GBM treatment. We also suggest that this effect is selective to GBM cells and occurs via PI3K/Akt pathway inhibition. Finally, Magnetic resonance imaging (MRI) revealed that a non-cytotoxic concentration of EqTx-II potentiates the VP-16-induced inhibition of GBM growth in vivo. These combined therapies constitute a new and potentially valuable tool for GBM treatment, leading to the requirement of lower concentrations of chemotherapeutic drugs and possibly reducing, therefore, the adverse effects of chemotherapy.
Dissolution Behaviors of Copper Metal in Alkaline H2O2-EDTA Solutions. (1993)

Abstract The dissolution behavior of Cu metal in hydrogen peroxide (H2O2)-ethylenediaminetetraacetic acid (EDTA) solution was studied through both electrochemical and chemical tests. In the electrochemical experiments, the open circuit potential (OCP) of Cu electrode at low H2O2concentration below 0.5wt% was determined by Cu anodic reaction and H2O2 cathodic reaction. On the other hand, the OCP in higher concentration above 2 w/t% of H2O2 approached to 70 mV vs. SCE, since the redox reaction of H2O2 was so dominant reaction that the Cu anodic reaction was masked. The anodic polarization curve of Cu around the OCP of 70 mV in the range between -200 and 200 mV in the solution without H2O2 showed the maximum Cu dissolution reaction front, while those of both carbon steel and Inconel 600 in the same potential range were situated in the passivation region. The anodic polarization behaviors of Cu in the H2O2 concentrations above 1 wt% were examined by a potentiostatic method along with a weight loss measurement. The anodic Cu dissolution current estimated from the weight loss measurement in the solution containing more than 1 wt% of H2O2 was higher than that predicted by the polarization curve of Cu in the H2O2-free solution. The dissolution reaction rate of Cu in the H2O2 solution linearly increased with H2O2 concentration. This result indicated that the dissolution reaction rate of Cu in the presence of H2O2 was enhanced by a reaction between Cu and H2O2, resulting in the shift of the polarization curves depending on H2O2 concentration. The experimental correlation equation based on the above results described well the overall dissolution reaction rate of Cu in H2O2 concentration ranges below 1 wt% and above 2 wt% in the EDTA solution.
A practical approach to wideband antenna array characterization (2002)

A high power HF phased antenna array capable of very wideband frequency and scan coverage is being constructed as part of the High Frequency Active Auroral Research Program (HAARP), in Galkona, AK. This paper presents measurement and analytic array characterization approach for the purpose of antenna evaluation and antenna matching unit (AMU) design. It is based on the use of scattering parameters measurement of the array and has resulted in a 30% improvement in the low frequency range of the HAARP system. The effectiveness of the design is further evaluated by calculating the average efficiency of the array system, which is within 1.5 dB of its theoretical maximum.
Lipid droplet accumulation occurs early following Salmonella infection and contributes to intracellular bacterial survival and replication (2021)

Salmonellosis is a public health problem caused by Salmonella sp., a highly adapted facultative intracellular pathogen. After internalization, Salmonella sp. Manipulates several host processes, mainly through the activation of the type III secretion system (T3SS), including modification of host lipid metabolism and lipid droplet (LD) accumulation. LDs are dynamic and complex lipid-rich organelles involved in several cellular processes. The present study investigated the mechanism involved in LD biogenesis in Salmonella-infected macrophages and its role in bacterial pathogenicity. Here, we reported that S. Typhimurium induced a rapid time-dependent increase of LD formation in macrophages. The LD biogenesis was demonstrated to depend on Salmonella's viability and SPI1-related T3SS activity, with the participation of Toll-Like Receptor (TLR) signaling. We also observed that LD accumulation occurs through TLR2-dependent signaling and is counter-regulated by TLR4. Last, the pharmacologic modulation of LD formation by inhibiting diacylglycerol O-acyltransferase 1 (DGAT1) and cytosolic phospholipase A2 (cPLA2) significantly reduced the intracellular bacterial proliferation and impaired the prostaglandin E2 (PGE<sub>2</sub> ) synthesis. Collectively, our data suggest the role of LDs on S. typhimurium intracellular survival and replication in macrophages. This data set provides new perspectives for future investigations about LDs in host-pathogen interaction.
MIC-1 Antlerogenic Stem Cells Homogenate from Cervus elaphus Accelerate Corneal Burn Reepithelization in Rabbits (2022)

Deer antler is the only mammalian organ that can fully grow back once lost from its pedicle. Antler regeneration is a stem cell-based process. Therefore, antlers probably offer the most pertinent model for studying organ regeneration in mammals. Evaluation of the effect of deer antler stem cells on the healing of superficial and deep rabbit corneal wounds was performed. Thirty-six New Zealeand White rabbits were used in this study in superficial and deep denaturation models, and corneal erosion was performed with n-heptanol placed on the cornea for 30 and NaOH for 90 s. Antler stem cells in drop formulation with hyaluronate was used. As a control, sodium hyaluronate in the superficial model and protein-free calf blood dialysate (Solcoseryl) in the deep model were administered. In superficial corneal damage, a reduction in the area of the damaged cornea was observed from day 3 of the experiment to an adequate level: 45% in the test group and 52% in the control group relative to the baseline damage (100%). Between days 3 and 7, on average, a smaller lesion area was observed in the group receiving antler stem cells. The use of antler stem cells has resulted in a marked improvement in cornea clarity. According to the 5-point scale of corneal opacity evaluation, where 1 is completely clear and 5 is completely opaque, the first statistically significant changes were observed after 4 weeks of treatment: 3.0 in the study group, 4.1 in the control with Solcoseryl, and 4.4 in the control group. After 9 weeks, these values were, 2.5, 3.8, and 4.1, respectively. The present preliminary study shows the promising results of antlerogenic stem cells of Cervus elaphus topically applied for the treatment of corneal injury. A deeper understanding of the developmental mechanisms involved in antler renewal can be useful for controlling regeneration cornea processes.
The manipulation by statistical characteristics of broadband signal in telecommunications systems (2004)

Recently there has been stable interest in digital communications systems on the basis of noise-like carriers for increasing the secrecy, noise-immunity and protection from unauthorized access. A broadband communication channel model on the basis of information signal modulation of statistical parameters of noise-like signals was considered.