gebhart/patrepeval huggingface dataset:
Interference with Ca2+ release activated Ca2+ (CRAC) channel function delays T‐cell arrest in vivo (2013)

Entry of lymphocytes into secondary lymphoid organs (SLOs) involves intravascular arrest and intracellular calcium ion ([Ca(2+)]i) elevation. TCR activation triggers increased [Ca(2+)]i and can arrest T-cell motility in vitro. However, the requirement for [Ca(2+)]i elevation in arresting T cells in vivo has not been tested. Here, we have manipulated the Ca(2+) release-activated Ca(2+) (CRAC) channel pathway required for [Ca(2+)]i elevation in T cells through genetic deletion of stromal interaction molecule (STIM) 1 or by expression of a dominant-negative ORAI1 channel subunit (ORAI1-DN). Interestingly, the absence of CRAC did not interfere with homing of naïve CD4(+) T cells to SLOs and only moderately reduced crawling speeds in vivo. T cells expressing ORAI1-DN lacked TCR activation induced [Ca(2+)]i elevation, yet arrested motility similar to control T cells in vitro. In contrast, antigen-specific ORAI1-DN T cells had a twofold delayed onset of arrest following injection of OVA peptide in vivo. CRAC channel function is not required for homing to SLOs, but enhances spatiotemporal coordination of TCR signaling and motility arrest.
Phosphorylation of Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) by Akt Promotes Stability and Mitogenic Function of S-phase Kinase-associated Protein-2 (Skp2) (2014)

The regulation of the cell cycle by the ubiquitin-proteasome system is dependent on the activity of E3 ligases. Skp2 (S-phase kinase associated protein-2) is the substrate recognition subunit of the E3 ligase that ubiquitylates the cell cycle inhibitors p21(cip1) and p27(kip1) thus promoting cell cycle progression. Increased expression of Skp2 is frequently observed in diseases characterized by excessive cell proliferation, such as cancer and neointima hyperplasia. The stability and cellular localization of Skp2 are regulated by Akt, but the molecular mechanisms underlying these effects remain only partly understood. The scaffolding protein Ezrin-Binding Phosphoprotein of 50 kDa (EBP50) contains two PDZ domains and plays a critical role in the development of neointimal hyperplasia. Here we report that EBP50 directly binds Skp2 via its first PDZ domain. Moreover, EBP50 is phosphorylated by Akt on Thr-156 within the second PDZ domain, an event that allosterically promotes binding to Skp2. The interaction with EBP50 causes cytoplasmic localization of Skp2, increases Skp2 stability and promotes proliferation of primary vascular smooth muscle cells. Collectively, these studies define a novel regulatory mechanism contributing to aberrant cell growth and highlight the importance of scaffolding function of EBP50 in Akt-dependent cell proliferation.
Lack of functional caveolae in Cav3 mutated human dystrophic myotubes results in deficient mechanoprotection and IL6/STAT3 mechanosignaling (2018)

Abstract Caveolin-3 is the major structural protein of caveolae in muscle cells. Mutations in the CAV3 gene cause different type of muscle disorders mostly characterized by defects in membrane integrity and repair, deregulation in the expression of various muscle proteins and deregulation of several muscle associated signaling pathways. We show here that myotubes derived from patients bearing the CAV3 P28L and R26Q mutations present a lack of functional caveolae at the plasma membrane which results in an abnormal mechanoresponse. Mutant myotubes can no longer buffer the increase of membrane tension induced by mechanical stress and present an hyperactivation of the IL6/STAT3 signaling pathway at rest and under mechanical stress. The impaired mechanical regulation of the IL6/STAT3 signaling pathway by caveolae leads to chronic activation and a higher expression of muscle specific genes. These defects could be reversed by reassembling a pool of functional caveolae through expression of wild type Cav3. Our findings bring more mechanistic insight into human Cav3 associated muscle disorders and show a general defect in the mechanoresponse of CAV3 P28L and R26Q myotubes.
In Defense against Pathogens. Both Plant Sentinels and Foot Soldiers Need to Know the Enemy, (2003)

Plants are major targets of microbes seeking a source of nutrition. A complex array of interactions between plants and microbes has evolved that reflects both the nutrient acquisition strategies of microbes and defense strategies of plants. Part of plant defense strategy includes an active offense
Recurrent Nevus Phenomenon Developing within a Keloid (2023)

The recurrent nevus phenomenon represents the persistence of a nevus within a scar from a prior biopsy site, with the acquisition of clinical and histologic features frequently overlapping with those of melanoma, posing relevant diagnostic challenges. Similar features are recognized in nevi that have undergone recent or chronic trauma and in sclerosing nevi. Any type of nevus may be subject to this phenomenon. Keloids are exuberant scars with an exaggerated accumulation of dense dermal collagen. Here we report a case of a 42-year-old woman with the incidental finding of an atypical melanocytic proliferation developing within a keloidal scar. The patient presented with a progressively enlarging auricular lesion three years after a piercing procedure. Upon histological examination, attentive scrutiny of the margin revealed an atypical compound melanocytic proliferation, predominantly single-celled at the junction but occasionally nested, with cytologic atypia and architectural disorder. This atypical proliferation was found emerging above a keloid. We interpreted the lesion as an atypical melanocytic lesion with features resembling the recurrent nevus phenomenon. This case raises awareness in recognizing these melanocytic lesions as benign, thereby avoiding overdiagnosis and unnecessary treatment.
A Theoretical Framework for Traffic Speed Estimation by Fusing Low-Resolution Probe Vehicle Data (2011)

Probe vehicles with Global Positioning Systems (GPS) can provide accurate positions that enable spatial-average speed estimation. However, some probe vehicles cannot provide accurate positions but can provide location-specific information on when and where they are located at the segment or cell level. These topological position (TP) data with segment- or cell-level accuracy cannot provide the distance component that is necessary for traffic speed estimation. However, considering the wide availability of TP data in the existing telecommunications network, there is still hope and benefits to make use of the data for traffic state estimation. In this paper, an algorithm is proposed using low-resolution positioning data. The proposed method is capable of fusing low-resolution positioning data with other data sources, leading to more accurate and reliable speed estimation of relatively low bias. In addition, this method shows strong robustness and error tolerance and can reveal the magnitude of the estimation error, which is helpful for travel time prediction and traffic control.
DISASTER MANAGEMENT IN UNIVERSITIES FOR A LARGE-SCALE EARTHQUAKE (2012)

平成23年3月11日に東北地方で発生した東日本大震災を契機に，横浜国立大学では災害対策再構築の学内プロジェクト事業を推進した．筆者らは大学内の災害対策体制の強化のためプロジェクトワーキンググループを編成し，学内災害対策マニュアルの実質的な改訂と災害対応訓練を企画し，実施に協力した．本報告では，災害対策本部図上訓練および全学的避難訓練の実施並びに訓練参加者に対するアンケート結果を踏まえ，今後の大学における大規模地震に対する組織対応のあり方について検討した結果を報告する．
Challenges in Reengineering Automotive Software (2009)

Automotive software is different from the kind of software that is usually addressed by current reengineering research. This paper gives an overview of the particular conditions and challenges that arise in the automotive domain.
Study of a Hybrid Excitation Synchronous Machine: Modeling and Experimental Validation (2019)

This paper deals with a parallel hybrid excitation synchronous machine (HESM). First, an expanded literature review of hybrid/double excitation machines is provided. Then, the structural topology and principles of operation of the hybrid excitation machine are examined. With the aim of validating the double excitation principle of the topology studied in this paper, the construction of a prototype is presented. In addition, both the 3D finite element method (FEM) and 3D magnetic equivalent circuit (MEC) model are used to model the machine. The flux control capability in the open-circuit condition and results of the developed models are validated by comparison with experimental measurements. The reluctance network model is created from a mesh of the studied domain. The meshing technique aims to combine advantages of finite element modeling, i.e., genericity and expert magnetic equivalent circuit models, i.e., reduced computation time. It also allows taking the non-linear characteristics of ferromagnetic materials into consideration. The machine prototype is tested to validate the predicted results. By confronting results from both modeling techniques and measurements, it is shown that the magnetic equivalent circuit model exhibits fairly accurate results when compared to the 3D finite element method with a gain in computation time.
KEYNOTE-119: A randomized phase III study of single-agent pembrolizumab (MK-3475) vs single-agent chemotherapy per physician's choice for metastatic triple-negative breast cancer (mTNBC). (2016)

TPS1102 Background: Pembrolizumab is a humanized monoclonal antibody against PD-1 that blocks the PD-1–ligand interaction, allowing activation of antitumor immune response. In the phase 1b KEYNOTE-012 study, pembrolizumab showed promising antitumor activity and manageable safety in heavily pretreated patients with mTNBC, supporting further evaluation of pembrolizumab in these patients. The randomized, global phase III KEYNOTE-119 study (NCT02555657) was designed to compare the efficacy and safety of pembrolizumab monotherapy with those of investigator’s choice chemotherapy in patients receiving second-line or third-line treatment for mTNBC. Methods: Eligible patients must have centrally confirmed TNBC, have received 1 or 2 prior systemic treatments for metastatic breast cancer, have documented progression on most recent therapy, and have been previously treated with an anthracycline and/or a taxane. Patients are randomly assigned 1:1 to receive pembrolizumab 200 mg Q3W or single-agent chemotherapy per investigator’s choice: capecitabine, eribulin, gemcitabine, or vinorelbine. Randomization is stratified by PD-L1 tumor status and history of prior neo/adjuvant treatment versus de novo metastatic disease at initial diagnosis. Treatment will continue for 35 cycles or until progressive disease (PD), unacceptable toxicity, or patient/investigator decision. Response will be assessed every 9 wk up to 1 yr after randomization, and every 12 wk thereafter, based on RECIST v1.1 per central review and RECIST adapted for immunotherapy response patterns. Clinically stable patients may continue treatment until PD confirmation at least 4 wk after initial evidence of progression. AEs will be assessed throughout treatment and for 30 d thereafter (90 d for serious AEs) and graded per NCI CTCAE v4.0. After progression, patients will be followed for survival every 3 mo. Primary end points are OS and PFS based on RECIST v1.1 by central review; secondary end points include safety, ORR, duration of response, and disease control rate. Enrollment in KEYNOTE-119 will continue until ~600 patients have enrolled. Clinical trial information: NCT02555657.
Laboratory study of carbon particles and clusters (1993)

To investigate the role of pure carbon in interstellar absorption, optical spectroscopy on laboratory-produced carbon grains, C60 Buckminsterfullerene and small carbon clusters has been carried out. The results obtained so far are: (a) The UV absorption of small (diameter ca. 10 nm) carbon grains depends strongly on the atomic structure of the grain material. A fit of the laboratory data to the interstellar 217 nm feature by graphitic grains seems to be possible provided some serious experimental problems can be overcome. (b) There is so far no clear-cut coincidence between the measured spectra of neutral and ionised C60 and the known interstellar bands in the UV and IR. (c) The spectra of carbon clusters (C5–ca. C11) trapped in cryogenic matrices show great similarities to the pattern of diffuse interstellar bands in the visible range.
Studio dei semiochimici intraspecifici ed interspecifici per il controllo di Cacopsylla pyri (L.). (2014)

Cacopsylla pyri (L.) (Hemiptera, Psyllidae) è uno degli insetti più dannosi al pero; il suo mancato controllo provoca significative riduzioni quali-quantitative della produzione. Cacopsylla pyri produce melata che causa sviluppo di fumaggini, necrosi di tessuti fogliari e frutti, ed è responsabile della trasmissione del fitoplasma “Candidatus Phytoplasma pyri’’. Il contenimento del fitomizo è spesso insoddisfacente, soprattutto in assenza del suo predatore naturale, Anthocoris nemoralis. Al fine di promuovere nuove strategie di controllo biotecnico, efficaci e di minor impatto ambientale, sono stati intrapresi studi elettrofisiologici e comportamentali per valutare l’attività biologica di sostanze presenti negli estratti cuticolari di adulti di C. pyri e di composti volatili di origine vegetale emessi da diverse cultivar di pero..Le risposte EAG hanno evidenziato che i composti volatili presenti negli estratti di femmine e maschi di C. pyri, somministrati a 3 dosi crescenti, stimolano le antenne di entrambi i sessi, anche se con diversa intensità.L’analisi gascromatografica abbinata a spettrometria di massa (GC-MS) su estratti cuticolari di femmine e maschi ha permesso l’identificazione, tra gli altri, di quattro composti alifatici a lunga catena.I quattro composti identificati, presenti in quantità maggiori nella cuticola delle femmine rispetto a quella dei maschi, sono i più probabili candidati quali componenti della miscela feromonica di C. pyri.Tra i tanti composti volatili emessi dalle diverse cv di pero, tutti quelli saggiati sulle antenne di femmine e maschi di C. pyri hanno evocato risposte EAG evidenti quantificabili; tali risposte, generalmente maggiori nelle femmine, sono risultate significativamente più elevate solo per alcune aldeidi . Tali composti potrebbero essere utilizzati, ad esempio, nel monitoraggio delle femmine di C. pyri, per modificarne il comportamento nella scelta della pianta ospite, del sito di ovideposizione, o per potenziare l’attrattività del feromone sessuale.
Viscoelastic Modeling of Template-Directed DNA Synthesis (2005)

In the present study, we have used the QCM-D technology to study the replication of surface attached oligonucleotide template strands using Escherichia coli DNA polymerase I (Klenow fragment, KF). Changes in resonance frequency (F) and energy dissipation (D) for DNA hybridization and polymerization were recorded at multiple harmonics. Formation of the polymerase/DNA complex led to a significant decrease in energy dissipation, which is consistent with a conformational change induced upon enzyme binding. This interpretation was further strengthened by a data analysis using a Voigt-based viscoelastic model. The analysis revealed a significant increase in shear viscosity and shear modulus during KF binding, whereas the viscoelastic properties of single- and double-stranded templates were almost identical. During the actual DNA synthesis, an initial increase in rigidity (shear viscosity) was followed by a gradual decrease that has two components corresponding to the release of enzyme and to the presence of the catalytically active enzyme/substrate complex. The corresponding decrease in surface concentration was found to underestimate the rate of enzyme release due to viscously coupled water that compensates for the loss in enzyme mass. Furthermore, the modeling elucidates that significant changes in both F and D originate from variations in the viscoelastic properties, which means that changes in F alone should be used with care for estimations of coupled mass and kinetics. Therefore, the modeled temporal variation in effective thickness, being proportional to coupled mass and, thus, independent of structural changes, was used to estimate the catalytic constants of the polymerization reaction. The reported work is the first example providing this type of structural information for the catalytic action of an enzyme, thereby demonstrating the potential of the technique for advanced analysis of complex biological reactions, including proper analysis of enzyme kinetics.
Sup Fig 3 from New Mechanisms of Resistance to MEK Inhibitors in Melanoma Revealed by Intravital Imaging (2023)

&lt;p&gt;Structural reorganization and amelanotic intratumoral nests are evident in persistent melanoma on MEKi.&lt;/p&gt;
Vision-based fuzzy controllers for navigation tasks (2002)

This paper deals with vision-based fuzzy closed-loop control schemes for collision avoidance as well as maintenance of clearance in a-priori unknown textured environments. These control schemes employ a visual motion cue, we call the visual threat cue (VTC) that provides some measure for a relative change in range as well as clearance between 3D surface and a fixated observer in motion. It is a collective measure obtained directly from the raw data of gray level images and is independent of the type of 3D surface texture. This motion cue is scale-independent, rotation independent, needs no 3D reconstruction and is measured in [time/sup -1/] units. Fuzzy control is closer in spirit to human thinking and can implement linguistically-expressed heuristic control policies directly without any knowledge about the dynamics of the complex process. The fuzzy controllers were implemented in real-time using a 486-based personal computer and a camera capable of undergoing 6-DOF motion. Results are highly encouraging.
Dual-Source Parallel RF Transmission for Clinical MR Imaging of the Spine at 3.0 T: Intraindividual Comparison with Conventional Single-Source Transmission (2010)

While shortening examination times by approximately one-third, the dual-source parallel RF transmission mode in MR imaging of the spine yielded diagnostic image quality comparable to that with the conventional single-source RF transmission mode.
Rate-Adaptive Atrial Pacing for Heart Failure With Preserved Ejection Fraction (2023)

ClinicalTrials.gov Identifier: NCT02145351.
Flott floppen die Pflöcke (1985)

Bild 1,1 zeigt das englische Pflock-Brett, auf dem das Pflock-Solitaire meist gespielt wird. Beim Auffüllen des Bretts tut man sich mit Murmelkugeln leichter, aber wenn es darum geht, das Spiel zu analysieren, bewähren sich die Pflöcke, weil sie besser haften.
Highly Modular Construction of Differently Substituted Dihydrodibenzo[a,c]cycloheptenes: Fast and Efficient Access to Derivatives of 2,2′‐Cyclo‐7,8′‐neolignans (2004)

Tying the knot: Two aryl units are linked in a one-pot reaction. In a subsequent MoCl5-mediated oxidative cyclization dihydrodibenzo[a,c]cycloheptenes are provided in excellent yields and an unusually broad range of functional groups are tolerated (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z53597_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Planar inverted F antenna loaded with high permittivitymaterial (1995)

Enhanced gain of an inverted F antenna loaded with a very high permittivity substrate has been demonstrated. Measurements show that the size of the antenna can be reduced without sacrificing the antenna radiation efficiency. The gain-enhancement mechanism, bandwidth, and antenna radiation gain and patterns are presented and discussed.
Intrinsic Fabry-Perot Interferometeric Sensor Based on Microfiber Created by Chemical Etching (2014)

An intrinsic Fabry-Perot interferometeric sensor based on a microfiber has been demonstrated. The micro-size suspended core is created by chemical etching a photonics crystal fiber, of which the cladding has a micrometer-spaced, hexagonal array of air holes. The sensing head is fabricated by chemical etching a short section of photonics crystal fiber spliced with a single mode fiber. The temperature sensing characteristic of the interferometer has also been demonstrated and a sensitivity 14.3 pm/°C is obtained.
Simulation of Aeroacoustic Sources in Aircraft Climate Control System (2006)

In the present study, the acoustic source generated by the flow inside internal ducts is studied using the Large Eddy Simulation technique. The cases under study correspond to two typical components used in the aircraft climate control system. The configurations are the case of a double diaphragm and a flow splitting device with a bifurcation. The two configurations differ in the acoustics noise genera tion mechanisms and in the regime of the flow. Both simulations are performed with FLUENT V6.2 general-purpose unstructured control-volume code. For both cases the unsteady si mulations give insight into the noise production mechanisms. For the first configuration, turbulent eddies detached from the first diaphragm impact the second diaphragm where strong wall pressure fluctuations are generated. For the second case, the unsteady calcul ation reveals that eddies formed at separation are convected along the opposite wall where they induce significant wall pressure fluctuations. The detailed comparison between the e xperimental pressure spectra and the results obtained with the Large Eddy Simulation shows a reasonable agreement in both cases. Both the tonal frequencies and broadband noi se are reproduced by the simulation for the double diaphragm case. In the bifurcation case where no tonal peaks are measured, the simulation predicts reasonably well the broadband noise for the low frequency range. It is shown that contrary to the double diaphragm case, results are very sensitive to the inlet turbulent boundary conditions treatment. The work described in this paper is part of an evaluation into the use of CAA methods for aircraft climate control systems at Airbus.
ChemInform Abstract: SINGLE‐FLASK SYNTHESES OF TETRASUBSTITUTED CYCLOPROPENES (1974)

Abstract Die Umsetzung von disubstituierten Acetylenen (I) mit Benzalchlorid (II) und überschüssigem Methyllithium (III), das sowohl als Metallierungsreagens als auch als Methylierungsreagens wirkt, in Äther bei 0°C führt zu einem Gemisch der tetrasubstituierten Cyclopropene (IV) und (V).
Acetogenine Isochinolin‐Alkaloide, 5. Aufbau und Cyclisierung terminal geschützter β‐Pentaketone: Erste Synthese des Aloenin‐Aglycons (1985)

Abstract Das Monoacetal 6b und das Diacetal 6c , Derivate des freien β‐Pentaketons 6a , werden aus einfachen Vorstufen durch Esterkondensation dargestellt. Die spezifische Cyclisierung von 6b zu dem Resorcin‐Derivat 15a wird zu einem ersten Syntheseweg zum Aglycon 2b des biologisch aktiven Bitterglucosids Aloenin ( 2a ) ausgebaut. Noch enger an der Biosynthese orientiert ist der dadurch besonders stufenarme zweite Zugang zu 2b über die nur minimal geschützte β‐Polycarbonylsäure 20 .
Uganda: Report of the Commission on Marriage, Divorce and the Status of Women (1966)

The programme of the dipldme d''etudes speciales in customary law will include (a) two compulsory subjects: special questions of customary law; introduction to Islamic law; (b) two optional subjects.