gebhart/patrepeval huggingface dataset:
The Activity of Microsomal Triglyceride Transfer Protein Is Essential for Accumulation of Triglyceride within Microsomes in McA-RH7777 Cells (1999)

Previously, based on distinct requirement of microsomal triglyceride transfer protein (MTP) and kinetics of triglyceride (TG) utilization, we concluded that assembly of very low density lipoproteins (VLDL) containing B48 or B100 was achieved through different paths (Wang, Y. , McLeod, R. S., and Yao, Z. (1997) J. Biol. Chem. 272, 12272-12278). To test if the apparent dual mechanisms were accounted for by apolipoprotein B (apoB) length, we studied VLDL assembly using transfected cells expressing various apoB forms (e.g. B64, B72, B80, and B100). For each apoB, enlargement of lipoprotein to form VLDL via bulk TG incorporation was induced by exogenous oleate, which could be blocked by MTP inhibitor BMS-197636 treatment. While particle enlargement was readily demonstrable by density ultracentrifugation for B64- and B72-VLDL, it was not obvious for B80- and B100-VLDL unless the VLDL was further resolved by cumulative rate flotation into VLDL(1) (S(f) > 100) and VLDL(2) (S(f) 20-100). BMS-197636 diminished B100 secretion in a dose-dependent manner (0.05-0.5 microM) and also blocked the particle enlargement from small to large B100-lipoproteins. These results yield a unified model that can accommodate VLDL assembly with all apoB forms, which invalidates our previous conclusion. To gain a better understanding of the MTP action, we examined the effect of BMS-197636 on lipid and apoB synthesis during VLDL assembly. While BMS-197636 (0.2 microM) entirely abolished B100-VLDL(1) assembly/secretion, it did not affect B100 translation or translocation across the microsomal membrane, nor did it affect TG synthesis and cell TG mass. However, BMS-197636 drastically decreased accumulation of [(3)H]glycerol-labeled TG and TG mass within microsomal lumen. The decreased TG accumulation was not a result of impaired B100-VLDL assembly, because in cells treated with brefeldin A (0.2 microgram/ml), the assembly of B100-VLDL was blocked yet lumenal TG accumulation was normal. Thus, MTP plays a role in facilitating accumulation of TG within microsomes, a prerequisite for the post-translational assembly of TG-enriched VLDL.
Delayed development of a pseudoaneurysm in the left circumflex artery following angioplasty and stent placement, treated with intravascular ultrasound-guided stenting (1997)

Three months following satisfactory angioplasty and stent placement, a patient developed restenosis and a pseudoaneurysm of a coronary artery segment proximal to the previously stented area. The restenosis and the pseudoaneurysm were successfully treated with intravascular ultrasound-guided primary stenting. The delay in the appearance of the pseudoaneurysm attests to the chronic nature of the lesion.
Preparation of High Jc Bi-Sr-Ca-Cu-O Bulk Sample by Floating Zone Method (1989)

Bi-Sr-Ca-Cu-O bulk samples with three different nominal compositions of Bi 2 Sr 2 CaCu 2 O y , Bi 2 Sr 2 Ca 2 Cu 3 O y and Bi 0.7 Pb 0.3 SrCaCu 1.8 O y were prepared by the floating zone method at growth rates ranging from 2 mm/h to 70 mm/h. The sample grown at 2 mm/h with the nominal composition of Bi 2 Sr 2 CaCu 2 O y possessed high density, highly aligned morphology, and a transport critical current density higher than 1490 A/cm 2 at 77 K under a zero magnetic field without any postannealing.
Tantalum Film Capacitors with Improved AC Properties (1978)

Tantalum thin-film capacitors have been fabricated from magnetron sputtered tantalum films with different nitrogen concentrations. Capacitors made from film containing between 13 and 22 atom percent nitrogen and heat treated at temperatures between 250°C and 350°C show lower ac losses and TCC, and improved capacitance stability, when compared to standard capacitors based on tantalum containing 3 atom percent nitrogen. In addition, the dc properties of the improved capacitors are equivalent or better than those of the standard product. It is proposed that in capacitors containing over 13 atom percent nitrogen, the nitrogen fills interstitial vacancies in the tantalum lattice. A barrier is therefore produced to substantial oxygen diffusion from the dielectric into the underlying tantalum film during exposure to temperature between 250° and 350°C. In addition, results at 200°C suggest that a second mechanism for improved characteristics is the removal of water or citrate molecules, incorporated during anodization, from the hulk of the dielectric.
Finite repetition threshold for large alphabets (2014)

\n We investigate the finite repetition threshold for k-letter alphabets, k ≥ 4, that is the smallest number r for which there exists an infinite r+-free word containing a finite number of r-powers. We show that there exists an infinite Dejean word on a 4-letter alphabet (i.e. a word without factors of exponent more than 7/5\n <!--inline-formula>\n <alternatives>\n <tex-math id="tex_eq1"><![CDATA[\\hbox{$\\frac{7}{5}$}]]></tex-math>\n <inline-graphic mime-subtype="png" mimetype="image" xlink:href="ita140010-eq6.png"/>\n <mml:math id="mml_eq1" type="inline">\n <mml:mrow>\n <mml:mfrac>\n <mml:mn mathvariant="normal">7</mml:mn>\n <mml:mn mathvariant="normal">5</mml:mn>\n </mml:mfrac>\n </mml:mrow>\n </mml:math>\n </alternatives>\n </inline-formula-->) containing only two 7/5\n <!--inline-formula>\n <alternatives>\n <tex-math id="tex_eq2"><![CDATA[\\hbox{$\\frac{7}{5}$}]]></tex-math>\n <inline-graphic mime-subtype="png" mimetype="image" xlink:href="ita140010-eq6.png"/>\n <mml:math id="mml_eq2" type="inline">\n <mml:mrow>\n <mml:mfrac>\n <mml:mn mathvariant="normal">7</mml:mn>\n <mml:mn mathvariant="normal">5</mml:mn>\n </mml:mfrac>\n </mml:mrow>\n </mml:math>\n </alternatives>\n </inline-formula-->-powers. For a 5-letter alphabet, we show that there exists an infinite Dejean word containing only \n 60 5/4\n <!--inline-formula>\n <alternatives>\n <tex-math id="tex_eq3"><![CDATA[\\hbox{$\\frac{5}{4}$}]]></tex-math>\n <inline-graphic mime-subtype="png" mimetype="image" xlink:href="ita140010-eq9.png"/>\n <mml:math id="mml_eq3" type="inline">\n <mml:mrow>\n <mml:mfrac>\n <mml:mn mathvariant="normal">5</mml:mn>\n <mml:mn mathvariant="normal">4</mml:mn>\n </mml:mfrac>\n </mml:mrow>\n </mml:math>\n </alternatives>\n </inline-formula-->-powers, and we conjecture that this number can be lowered to 45. Finally we show that the finite repetition threshold for k letters is equal to the repetition threshold for k letters, for every k ≥ 6.\n
Stereocontrolled total synthesis of lipoxins A (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereocontrolled total synthesis of lipoxins AK. C. Nicolaou, C. A. Veale, S. E. Webber, and H. KaterinopoulosCite this: J. Am. Chem. Soc. 1985, 107, 25, 7515–7518Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/ja00311a048https://doi.org/10.1021/ja00311a048research-articleACS PublicationsRequest reuse permissionsArticle Views2002Altmetric-Citations140LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Observation of X-rays generated by relativistic electrons in waveguide target mounted inside a betatron (2006)

In this work we have observed x-ray emission from x-ray waveguide radiator excited by relativistic electrons. The experiment carried out at Tomsk betatron B-35. Such new type stratified target was mounted on goniometer head inside the betatron toroid. The target is consisted of the W-C-W layers placed on Si substrate. The photographs of the angular distributions of the radiation generated in the target by 20-33 MeV electrons have shown the waveguide effect of the three-layer structure on x-rays generated in the target. The effect proved in an angular distribution of radiation as an additional narrow peak of guided x-rays intensity inside a wide cone of usual Bremsstrahlung.
Connecting the Mobile Phone with the Internet of Things - Benefits of EPC and NFC Compatibility (2007)

This paper describes the Electronic Product Code (EPC) and Near Field Communication (NFC) technologies.It lists the involved industry standards and elaborates on their commonalities and compatibility issues.The paper discusses the combination of EPC and NFC as a means to unleash the Internet of Things into the hands of the consumer.The authors investigate the benefits of enabling individuals to read EPC tags with mobile phones and detail the steps necessary to achieve that goal.If both technologies can be brought to work together in the future, it could positively influence consumers' perception of Radio Frequency Identification (RFID).
Isolation, Enumeration, and Maintenance of Rumen Anaerobic Fungi in Roll Tubes (1981)

Anaerobic phycomycetous fungi have been isolated from rumen fluid by using the Hungate roll tube technique. Cultures on solid substrates in roll tubes can be stored for long periods, and fungal zoospores can be enumerated in roll tubes.
A Millimeter-Wave Wide Band, Wide Scanning Phased Array-Fed Reflector Architecture (2021)

Phased Array Fed Reflectors (PAFRs) are desirable in RF antenna systems because of their high gain, however they require mechanical gimballing to compensate for the limited electronic scan volume that hinders swath. State of the art Electronically Scanned Array (ESA) solutions can address demanding link performance, capacity, and data rates using multiple agile analog beams, but they do so at a premium size, weight, power, and cost (SWaP-C). Especially for wide field of view (FOV) millimeter Wave (mmW) frequencies which require larger sized ESAs to close the links. This paper presents an innovative phased array architecture leveraging a ring-focused reflector that improves the scan performance of conventional PAFRs, denoted Wide Angle ESA Fed Reflector (WAEFR). This type of reflector architecture provides wide band performance with an electronic scan volume increase of up to 20x over traditional PAFRs and can reduce the size of an active ESA by over 40%.
Genomic Organization and Molecular Evolution of the Genes for Neuroglobin and Cytoglobin in the Hypoxiatolerant Israeli Mole Rat, Spalax Carmeli (2006)

The genes for the two respiratory proteins neuroglobin (Ngb) and cytoglobin (Cygb) in the subterranean Israeli mole rat Spalax carmeli have been sequenced and compared to other mammals including human. Coding regions of both Spalax genes are highly conserved on the nucleotide and amino acid level. The ratios of non-synonymous to synonymous nucleotide substitutions suggest strong purifying selection acting on Ngb and Cygb in all mammals. Thus, there appears to be no special sequence level adaptation in the two respiratory proteins within the hypoxia-tolerant mole rat. On the genomic level, Spalax Ngb and Cygb gene regions revealed the conserved 4-exon-3-intron structure and conserved CpG-rich islands in the 5' region. The Spalax Cygb gene promoter contains a conserved hypoxia-responsive transcription factor binding site, indicating a possible up-regulation of Cygb under oxygen deprivation. In Cygb intron 1, we observed a stretch of highly conserved putatively non-coding sequence of yet unknown (regulatory?) importance. In the Spalax Ngb gene, we note the presence of candidate hypoxia-responsive elements, which are not conserved in Ngb of hypoxia-sensitive mammals. Both globin gene regions harbor Spalax -specific simple sequence regions, which might be of adaptive value. We conclude that adaptations for hypoxia in mole rats are most likely to be found in regulatory functions rather than in protein structure.
Laser-Induced Optical Kerr Effect and the Dynamics of Orientational Order in the Isotropic Phase of a Nematogen (1973)

The transient order induced in the isotropic phase of the $N\ensuremath{-}(p\ensuremath{-}\mathrm{M}\mathrm{e}\mathrm{t}\mathrm{h}\mathrm{o}\mathrm{x}\mathrm{y}\mathrm{b}\mathrm{e}\mathrm{n}\mathrm{z}\mathrm{y}\mathrm{l}\mathrm{i}\mathrm{d}\mathrm{e}\mathrm{n}\mathrm{e})\ensuremath{-}p\ensuremath{-}\mathrm{b}\mathrm{u}\mathrm{t}\mathrm{y}\mathrm{l}\mathrm{a}\mathrm{m}\mathrm{i}\mathrm{n}\mathrm{e}$ by the plane-polarized electric field of a $Q$ -switched laser beam, is experimentally measured and analyzed. Good agreement is found with considerations deduced from the theory of de Gennes. It allows direct measurements of the optical Kerr constant and of the orientational relaxation time in these materials.
Lysozyme‐lysozyme self‐interactions as assessed by the osmotic second virial coefficient: Impact for physical protein stabilization (2009)

The purpose of the presented study is to understand the physicochemical properties of proteins in aqueous solutions in order to identify solution conditions with reduced attractive protein-protein interactions, to avoid the formation of protein aggregates and to increase protein solubility. This is assessed by measuring the osmotic second virial coefficient (B(22)), a parameter of solution non-ideality, which is obtained using self-interaction chromatography. The model protein is lysozyme. The influence of various solution conditions on B(22) was investigated: protonation degree, ionic strength, pharmaceutical relevant excipients and combinations thereof. Under acidic solution conditions B(22) is positive, favoring protein repulsion. A similar trend is observed for the variation of the NaCl concentration, showing that with increasing the ionic strength protein attraction is more likely. B(22) decreases and becomes negative. Thus, solution conditions are obtained favoring attractive protein-protein interactions. The B(22) parameter also reflects, in general, the influence of the salts of the Hofmeister series with regard to their salting-in/salting-out effect. It is also shown that B(22) correlates with protein solubility as well as physical protein stability.
Concurrent execution of phase compensation and automatic focusing procedures for telecentric off-axis digital holographic microscopy (2023)

This contribution presents a joint phase compensation and autofocusing method for telecentric off-axis Digital Holographic Microscopy (DHM). Current challenges of off-axis DHM systems applied to in-vivo imaging are the automatic reconstruction of phase images without phase distortions while the specimens under research move within the volume, generating out-of-focus holograms. Although different proposals to tackle these challenges individually have been reported for static samples, in this proposal, both issues are solved concurrently with no additional user intervention. As a result, in-focus compensated phase images of the out-of-focus studied samples are obtained. The proposal has been validated using simulated data.
A Straightforward Synthesis of glyco‐2,7‐ and 2,8‐Dienes. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Batch Production of High-Quality Graphene Grids for Cryo-EM: Cryo-EM Structure of Methylococcus capsulatus Soluble Methane Monooxygenase Hydroxylase (2023)

Cryogenic electron microscopy (cryo-EM) has become a widely used tool for determining the protein structure. Despite recent technical advances, sample preparation remains a major bottleneck for several reasons, including protein denaturation at the air-water interface, the presence of preferred orientations, nonuniform ice layers, etc. Graphene, a two-dimensional allotrope of carbon consisting of a single atomic layer, has recently gained attention as a near-ideal support film for cryo-EM that can overcome these challenges because of its superior properties, including mechanical strength and electrical conductivity. Here, we introduce a reliable, easily implemented, and reproducible method to produce 36 graphene-coated grids within 1.5 days. To demonstrate their practical application, we determined the cryo-EM structure of <i>Methylococcus capsulatus</i> soluble methane monooxygenase hydroxylase (sMMOH) at resolutions of 2.9 and 2.5 Å using Quantifoil and graphene-coated grids, respectively. We found that the graphene-coated grid has several advantages, including a smaller amount of protein required and avoiding protein denaturation at the air-water interface. By comparing the cryo-EM structure of sMMOH with its crystal structure, we identified subtle yet significant geometrical changes at the nonheme diiron center, which may better indicate the active site configuration of sMMOH in the resting/oxidized state.
Biological control of Alternaria radicina in seed production of carrots with Ulocladium atrum (2004)

Black rot of carrots is caused by seed-borne Alternaria radicina. Biological control of seed infestation by treatments applied to plants in flower during seed production with the fungal antagonist Ulocladium atrum was investigated in laboratory and field experiments resulting in a reduction of seed infestation by A. radicina. No negative side effects of the antagonist on seed quality were found. The application of U. atrum as a strong competitor during substrate colonisation of senescing tissues may also be promising in seed production of various other crops, e.g. of onion, cabbage or sugar beet, where necrotrophic seed pathogens enter necrotic flower parts before establishment on seeds.
Determination of Iron and Copper in 18th and 19th Century Books by Flameless Atomic Absorption Spectroscopy (1978)

AbstractSeveral badly foxed books and a paper handmade in our laboratory were studied for the presence and location of iron and copper compounds. The iron content of foxing spots and clear paper areas in the books showed a significant difference. However, the copper content of these areas was not significantly different. On the other hand, in our handmade paper it was the copper content that varied significantly in these areas. Traditionally, these elements in paper have been analyzed by gravimetric and titrimetric analytical techniques, colorimetry, or flame atomic absorption. CRA (Carbon Rod Atomizer), used with atomic absorption equipment, offers a simple, rapid and extremely sensitive method of determining metals in cellulosic materials directly on the solid sample.
Transgenic Targeting of Recombinant Rabies Virus Reveals Monosynaptic Connectivity of Specific Neurons (2010)

Understanding how neural circuits work requires a detailed knowledge of cellular-level connectivity. Our current understanding of neural circuitry is limited by the constraints of existing tools for transsynaptic tracing. Some of the most intractable problems are a lack of cellular specificity of uptake, transport across multiple synaptic steps conflating direct and indirect inputs, and poor labeling of minor inputs. We used a novel combination of transgenic mouse technology and a recently developed tracing system based on rabies virus (Wickersham et al., 2007a,b) to overcome all three constraints. Because the virus requires transgene expression for both initial infection and subsequent retrograde transsynaptic infection, we created several lines of mice that express these genes in defined cell types using the tetracycline-dependent transactivator system (Mansuy and Bujard, 2000). Fluorescent labeling from viral replication is thereby restricted to defined neuronal cell types and their direct monosynaptic inputs. Because viral replication does not depend on transgene expression, it provides robust amplification of signal in presynaptic neurons regardless of input strength. We injected virus into transgenic crosses expressing the viral transgenes in specific cell types of the hippocampus formation to demonstrate cell-specific infection and monosynaptic retrograde transport of virus, which strongly labels even minor inputs. Such neuron-specific transgenic complementation of recombinant rabies virus holds great promise for obtaining cellular-resolution wiring diagrams of the mammalian CNS.
Platycodon grandiflorum A. De Candolle Ethanolic Extract Inhibits Adipogenic Regulators in 3T3-L1 Cells and Induces Mitochondrial Biogenesis in Primary Brown Preadipocytes (2015)

This study was designed to evaluate the effects of Platycodon grandiflorum A. DC. ethanolic extract (PG) on obesity in brown/white preadipocytes. The effect of PG on the differentiation and mitochondrial biogenesis of brown adipocytes is still not examined. An in vivo study showed that PG induced weight loss in mice with high-fat-diet-induced obesity. PG successfully suppressed the differentiation of 3T3-L1 cells by down-regulating cellular induction of the peroxisome proliferators activated receptor γ (PPARγ), CCAAT enhancer binding protein α (C/EBPα), lipin-1, and adiponectin but increasing expression of silent mating type information regulation 2 homologue 1 (SIRT1) and the phosphorylation of AMP-activated protein kinase α (AMPKα). The effect of PG on the adipogenic factors was compared with that of its bioactive compound platycodin D. In addition, PG increased expressions of mitochondria-related genes, including uncoupling protein 1 (UCP1), peroxisome proliferator activated receptor-coactivator 1 α (PGC1α), PR domain containing 16 (PRDM16), SIRT3, nuclear respiratory factor (NRF), and cytochrome C (CytC) in primary brown adipocytes. These results indicate that PG stimulates the differentiation of brown adipocytes through modulation of mitochondria-related genes and could offer clinical benefits as a supplement to treat obesity.
Numerical investigation of the nonlinear dynamics of interacting microbubbles (2016)

The successful application of MBs requires detailed understanding of the nonlinear behavior of microbubbles (MBs), especially in polydisperse clouds, where the dynamic of every individual MB affects the other MBs. However, there is not enough information on how the nonlinear oscillations of one MB influences the other and vice versa. In this work the dynamics of 2 and 3 interacting MBs of initial radii of 1μm&amp;lt;R0&amp;lt;4 μm are studied by investigating the pressure dependent resonance curves and the bifurcation diagrams of the MBs (1 MHz&amp;lt;f&amp;lt;15 MHz, 1 kPa&amp;lt;p&amp;lt;1 MPa) for cases of no interaction and interaction with varying MB-MB distances. Results show that, for small enough distances, the pressure dependent resonance frequencies (fr) and the pressure threshold for harmonic and SH fr decreases. The larger MB may force new peaks in the resonance curves of the smaller MB at fr, harmonic fr and SH fr of the larger MB. The larger MB can control the dynamics of the smaller MB and force the smaller MB to exhibit the same nonlinear behavior (e.g., ½, 1/3, 1/4 SH) as of the larger MB. The dynamics of a cloud of interacting MBs maybe controlled by controlling the dynamics of the larger MB in the population.