gebhart/patrepeval huggingface dataset:
Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members. (1994)

To examine the functions of ERM family members (ezrin, radixin, and moesin), mouse epithelial cells (MTD-1A cells) and thymoma cells (L5178Y), which coexpress all of them, were cultured in the presence of antisense phosphorothioate oligonucleotides (PONs) complementary to ERM sequences. Immunoblotting revealed that the antisense PONs selectively suppressed the expression of each member. Immunofluorescence microscopy of these ezrin, radixin, or moesin "single-suppressed" MTD-1A cells revealed that the ERM family members are colocalized at cell-cell adhesion sites, microvilli, and cleavage furrows, where actin filaments are densely associated with plasma membranes. The ezrin/radixin/moesin antisense PONs mixture induced the destruction of both cell-cell and cell-substrate adhesion, as well as the disappearance of microvilli. Ezrin or radixin antisense PONs individually affected the initial step of the formation of both cell-cell and cell-substrate adhesion, but did not affect the microvilli structures. In sharp contrast, moesin antisense PONs did not singly affect cell-cell and cell-substrate adhesion, whereas it partly affected the microvilli structures. These data indicate that ezrin and radixin can be functionally substituted, that moesin has some synergetic functional interaction with ezrin and radixin, and that these ERM family members are involved in cell-cell and cell-substrate adhesion, as well as microvilli formation.
CD40-Activated B Cell Cancer Vaccine Improves Second Clinical Remission and Survival in Privately Owned Dogs with Non-Hodgkin's Lymphoma (2011)

Cell-based active immunotherapy for cancer is a promising novel strategy, with the first dendritic cell (DC) vaccine achieving regulatory approval for clinical use last year. Manufacturing remains arduous, especially for DC vaccines, and the prospect of using cell-based immunotherapy in the adjuvant setting or in combination with chemotherapy remains largely untested. Here, we used a comparative oncology approach to test the safety and potential efficacy of tumor RNA-loaded, CD40activated B cells in privately owned dogs presenting with non-Hodgkin's lymphoma (NHL), a clinical scenario that represents not only a major problem in veterinary medicine but also a bona fide spontaneous animal model for the human condition. When administered to NHL dogs in remission after induction chemotherapy, CD40-B cells electroporated ex vivo with autologous tumor RNA safely stimulated immunity in vivo. Although chemotherapy plus CD40-B vaccination did not improve time-to-progression or lymphoma-specific survival compared to dogs treated with chemotherapy alone, vaccination potentiated the effects of salvage therapy and improved the rate of durable second remissions as well as subsequent lymphoma-specific survival following salvage therapy. Several of these relapsed dogs are now long-term survivors and free of disease for more than a year. Overall, these clinical and immunological results suggest that cell-based CD40 cancer vaccination is safe and synergizes with chemotherapy to improve clinical outcome in canine NHL. More broadly, our findings underscore the unique value of clinical investigations in tumor-bearing companion animals.
ELECTRODE POTENTIALS OF THORIUM TETRACHLORIDE IN ALKALI CHLORIDE MELTS (1964)

The electrode potentials of the system Th, ThCl 4 (KCl, NaCl) were measured at various concentrations of thorium chloride, and at temperatures between 670 °C and 850 °C, using a silver – silver chloride reference electrode. It was shown by a direct gravimetric method that the reaction,Th (metal) + ThCl 4(melt) = 2ThCl 2(in melt) ,postulated by previous investigators, did not take place.The activity coefficients, and the other partial molal properties of the solutions of ThCl 4 , in the equimolar mixture of potassium and sodium chlorides, were calculated as a function of temperature from the e.m.f. measurements.
Application of double voxel functional spectroscopy to event-related cognitive experiments (1999)

The hemodynamic response to functional activation can be regarded as the convolution of the neuronal response with an unknown kernel. As such, it introduces an intrinsic blurring that limits the attainable temporal resolution of functional magnetic resonance (fMR) techniques. This study demonstrates that by measurement of displacements in activation onsets between different types of trial, it is nevertheless possible to obtain a subsecond temporal accuracy in fMR. A single trial stimulation paradigm was adopted: a simple search task embedded in a longer period of visual flicker stimulation that produced reliable activations in the primary visual cortex and supplementary motor area. Data were acquired from both of these regions using double voxel functional spectroscopy.
Correction to: Type 1 diabetes susceptibility alleles are associated with distinct alterations in the gut microbiota (2018)

Following publication of the original article [1] it came to the attention of the Production Editor that Figs. 1 and 2 had not been replaced with the newly revised figures supplied by the authors (the originals being unusable due to poor quality image and text).
Stand alone or join forces? Stem cell therapy for stroke (2018)

Bone marrow-derived MSCs appear to be the optimal stem cell source for stroke therapy. Optimizing dosing, timing, and delivery route should guide the clinical application of bone marrow-derived MSCs.
F6: Circuit, systems and data processing for next-generation wearable and implantable medical devices (2016)

There are exciting new developments in health care wearables, fitness trackers and therapeutic implantable devices. This advancement is fueled, in part, by the miniaturization of sensors and associated electronics. One of the greatest opportunities in wearables and implanted devices is the wealth of data that can be extracted from these devices and the high-quality information that can emerge by combining data from multiple sources for effective utilization by physicians. Longitudinal data, generated by continuous monitoring of physiological and vital parameters, can increase therapy efficacy and lead to the invention of new biomarkers. However, continuous and unobtrusive monitoring requires extreme miniaturization and minimal dependence on energy constraints. This creates challenges on integrated circuits and requires new techniques directed towards lowering energy consumption and reducing area and volume. This forum will provide an overview of the challenges of the various systems, circuits and heterogeneous technologies that are essential for building next generation on- and in-body medical devices.
Discovery of the nitric oxide signaling pathway and targets for drug development (2009)

Nitric oxide is a multifunctional signaling molecule, intricately involved with maintaining a host of physiological processes including but not limited to host defense, neuronal communication and the regulation of vascular tone. Many of the physiological functions first ascribed to NO are mediated through its primary receptor, soluble guanylyl cyclase. Endogenous production of NO is a highly complex and regulated process involving the 5-electron oxidation of L-arginine requiring numerous substrates and cofactors. The production of a highly reactive and diffusible free radical gas further complicates our established concept and model of specific and targeted receptor-ligand interaction to elicit cell signaling events. Hence there are many steps in the endogenous pathway for altered production of NO and subsequent activation of sGC that may be targets for drug development as well as other molecular targets for NO. The following review will highlight the current state of the art of NO-sGC research and illustrate disease processes which may benefit from novel drug development exploiting the NO-sGC pathway as well as NOS and cGMP-independent pathways.
Fire Pit-Related Burn Injuries in Children and Adolescents (2019)

Thermal burns are a leading cause of preventable injury in children and adolescents. Fire pits have become increasingly popular outdoor fixtures in U.S. homes. We aimed to identify trends in pediatric burn injuries related to fire pits that presented to a representative sample of United States Emergency Departments (EDs). A retrospective analysis of annual ED visits from the National Electronic Injury Surveillance System (NEISS) from January 1, 2006 through December 31, 2017 using product codes specific to fire pits. U.S. Census population estimates were used to compute rates per 100,000 population. SAS and Joinpoint weighted regression analyses were used to analyze annual estimates and rate trends across the study period. There were 10,951 (95% CI = 8535-13,367) ED visits for burn injuries secondary to outdoor fire pits in patients 19 years of age and younger across the study period. The majority of injuries occurred in children under the age of 5, and were related to falls into or on a hot fire pit. The annual modeled rate change showed an overall significant increase in burn injuries of 7.16 per 100,000 annually from 2006 to 2017 (P = .02). Outdoor fire pits represent an increasing hazard to young children who are particularly susceptible to burn injuries from falls in or around lit recreational fires. Product modifications and public awareness campaigns are necessary to prevent future life-altering injuries in pediatric patients.
ASBMR 29th Annual Meeting (2007)

This free journal suppl. entitled: Supplement: 30th ASBMR Annual Meeting Advancing The Future
Characterization of a Mitochondrial Metallopeptidase Reveals Neurolysin as a Homologue of Thimet Oligopeptidase (1995)

We have isolated a metallopeptidase from rat liver. The peptidase is primarily located in the mitochondrial intermembrane space, where it interacts non-covalently with the inner membrane. The enzyme hydrolyzes oligopeptides, the largest substrate molecule found being dynorphin A1-17; it has no action on proteins, and does not interact with alpha 2-macroglobulin, and can therefore be classified as an oligopeptidase. We term the enzyme oligopeptidase M. Oligopeptidase M acts similarly to thimet oligopeptidase (EC 3.4.24.15) on bradykinin and several other peptides, but hydrolyzes neurotensin exclusively at the -Pro+Tyr- bond (the symbol + is used to indicate a scissile peptide bond) rather than the -Arg+Arg- bond. The enzyme is inhibited by chelating agents and some thiol-blocking compounds, but differs from thimet oligopeptidase in not being activated by thiol compounds. The peptidase is inhibited by Pro-Ile, unlike thimet oligopeptidase, and the two enzymes are separable in chromatography on hydroxyapatite. The N-terminal amino acid sequence of rat mitochondrial oligopeptidase M contains 19 out of 20 residues identical with a segment of rabbit microsomal endopeptidase and 17 matching the corresponding segment of pig-soluble angiotensin II-binding protein. Moreover, the rat protein is recognized by a monoclonal antibody against rabbit soluble angiotensin II-binding protein, all of which is consistent with these proteins being species variants of a single protein that is a homologue of thimet oligopeptidase. The biochemical properties of the mitochondrial oligopeptidase leave us in no doubt that it is neurolysin (EC 3.4.24.16), for which no sequence has previously been reported, and which has not been thought to be mitochondrial.
Get a grip: inward dactyl motions improve efficiency of sideways-walking gait for an amphibious crab-like robot (2022)

Crabs are adept at traversing natural terrains that are challenging for mobile robots. Curved dactyls are a characteristic feature that engage terrain in order to resist wave forces in surf zones. Inward gripping motions at the onset of the stance could increase stability. Here, we add inward gripping motions to the foot trajectories of walking gaits to determine the energetic costs and speed for our 12 degree of freedom (DOF) crab-like robot, Sebastian. Specifically, we compared two gaits in which the step size (stance length) was the same, but the swing trajectories were either triangular (to minimize trajectory length) or quadrilateral (in which the leg deliberately oversteps in order to perform a distributed inward grip). The resulting gripping quadrilateral gait significantly outperformed the nongripping triangular gait on diverse terrains (hard linoleum, soft mats, and underwater sand), providing between 15% and 34% energy savings. Using this gait eliminates the advantage of spherical end effectors for slip reduction on hard linoleum, which may lead to a better understanding of how to use crab-like morphology for more efficient locomotion. Finally, we subjected the walking robot to lab-generated waves with a wave height approximately 166% of the dactyl length. Both gaits enabled the robot to walk undisturbed by the waves. Taken together, these results suggest that impact trajectory will be key for future amphibious robots. Future work can provide a deeper understanding of the relationships between dactyls, gaits, and substrates in biology and robots.
DIGESTION DES PROTÉINES DE CÉRÉALES CHEZ L'AGNEAU EN CROISSANCE (1973)

In the presence of urea or soyabean protein, bacterial protein synthesis was further increa- sed.
Antibodies to the trypsin cleavage peptide VP8 neutralize rotavirus by inhibiting binding of virions to target cells in culture (1991)

Two distinct patterns of neutralization were identified by comparing the neutralization curves of monoclonal antibodies (MAbs) directed at the two surface proteins, VP4 and VP7, of rhesus rotavirus. VP7-specific MAbs were able to neutralize virus efficiently, and slight increases in antibody concentration resulted in a sharp decline in infectivity. On the other hand, MAbs to VP4 proved much less efficient at neutralizing rhesus rotavirus, and the fraction of infectious virus decreased gradually throughout a wide range of antibody concentrations. MAbs directed at VP8*, the smaller trypsin cleavage fragment of VP4, were shown to efficiently prevent binding of radiolabeled virions to MA104 cell monolayers, to an extent and at concentrations comparable to those required for neutralization of infectivity. Conversely, MAbs recognizing VP7 or the larger VP4 trypsin cleavage product, VP5*, showed little or no inhibitory effect on virus binding to cells. All MAbs studied were able to neutralize rotavirus that was already bound to the surface of cells. The MAbs directed at VP8*, but not those recognizing VP5* or VP7, were shown to mediate release of radiolabeled virus from the surface of the cells. With MAbs directed at VP7, papain digestion of virus-bound antibody molecules led to an almost complete recovery of infectivity. Neutralization could be fully restored by incubation of virus-Fab complexes with anti-mouse immunoglobulin G antiserum. Neutralization with MAbs directed at VP8* proved insensitive to digestion with papain as well as to the addition of anti-immunoglobulin antibodies.
Characterization of metal-clad TE/TM mode splitters using the finite element method (1997)

A metal-clad directional coupler-based polarizer with two-dimensional (2-D) modal confinement has been designed and its performance has been rigorously computed by using the accurate vector H-field finite element method after taking into consideration the polarization-dependent coupling lengths, phase mismatchings and losses due to metal-cladding of the system involved.
Exploring red blood cells as a novel tolerogenic approach for Factor VIII inhibitors employing immuno-dominant FVIII derived peptides presented on MHC class II (2023)

Introduction The main complication of hemophilia A treatment is the development of neutralizing antibodies (inhibitors) against factor VIII (FVIII). The eradication of anti-FVIII antibodies relies on immune tolerance induction (ITI). Since ITI is efficient in only 60-80% of cases, within the EDUC8-consortium we are developing innovative methods to reduce the immunogenicity of biotherapeutics. Here we employed bioinformatics and proteomic-based peptide presentation assays to identify promiscuously presented FVIII peptides to use in pioneering immuno-tolerogenic approaches. To this end, we are exploring red blood cells (RBCs) as innovative antigen delivery system to induce tolerance.
The influence of automated plateletpheresis on systemic levels of hematopoietic growth factors (1999)

The data show a coordinated response of the hematopoietic system to platelet loss. It is suggested that the decrease in serum stem cell factor levels during apheresis reflects the consumption of stem cell factor by early hematopoietic progenitors that expand to initiate early megakaryocytopoiesis. The temporary increase in thrombopoietin is the result of platelet loss and serves as a stimulus for subsequent thrombopoiesis. The pronounced elevation of erythropoietin after apheresis suggests a role for this primarily erythropoietic cytokine in thrombopoiesis, too.
Development of a Jones vector based model for the measurement of a plasma current in a thermonuclear fusion reactor with a POTDR setup (2012)

Fibre optical current sensor (FOCS) is a promising alternative to inductive sensors for the measurement of the plasma current in future thermonuclear fusion reactors. Standard FOCS relies on the measurement of the state of polarisation (SOP) of light at the output of an optical bre surrounding a current. Because of the Faraday eect, magnetic eld induced by electrical current rotates the SOP of light travelling into the bre. According to the Ampere's theorem this rotation is proportional to the surrounded current. In future tokamaks like ITER and DEMO, the plasma current will be suciently high to generate a rotation of the SOP higher than 2 radians. These conditions may lead to uncertainties on the determination of the plasma current if no post processing is performed. In this paper we propose a solution with a Polarisation Optical Time Domain Re ectometer (POTDR) setup allowing both unambiguous plasma current measurement and also local magnetic eld measurements. This measurement is based on the assessment of the SOP rotation of the Rayleigh backscattered POTDR signal. Thanks to the presence of an input polarizer, SOP variations are converted into power uctuations that contain information about the distribution of the magnetic eld and therefore about the plasma current. Using the Jones formalism we have developed a model accounting for the modication of the SOP of light travelling into the optical bre and the evolution of the POTDR signal. In parallel experimental PODTR measurements have been performed on the Tore Supra tokamak situated at CEA Cadarache in France. A comparison between the models and the experimental results conrms the capability of the system to measure the plasma current and the local magnetic eld even if further data post processing are still required.
Generation of extended pulse trains of minimum duration by passive negative feedback applied to solid-state Q-switched lasers (1992)

Intracavity self-focusing in a thin two-photon absorber acts, in combination with pinholes, as an efficient power and energy limiter in flashlamp (pulsed) and arc lamp (CW) pumped actively passively mode-locked crystalline Nd-doped lasers. Power limiting by the intracavity two-photon absorber is exploited to create the condition for optimum pulse compression in the saturable absorber. Additional pulse shortening is due to the elimination of the pulse tail by self-defocusing in the nonlinear crystal. Extracavity and intracavity measurements, as well as three-dimensional computer simulations, give a complete understanding of the pulse evolution in the cavity, including the two mechanisms of pulse shaping and compression.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Antimicrobial Susceptibility Testing Methods for Bacterial Pathogens (2009)

Gone are the days when the antimicrobial susceptibility pattern of a bacterial isolate could be predicted simply on the basis of its species identifi cation. Although Streptococcus pyogenes isolates remain susceptible to penicillin, one has to continually ask – for how long? With the discovery of strains of Staphylococcus aureus that are highly resistant to vancomycin (1) and strains of Acinetobacter species that are pan resistant (2, 3), the role of antimicrobial susceptibility testing in guiding therapy for infectious diseases is becoming more and more important (4). Yet, ironically, many of these novel resistance phenotypes are not easily detected using the automated susceptibility testing methods so prevalent in today’s clinical laboratories (5–7). The ability of the clinical laboratory to detect emerging resistance profi les is often directly related to the extra efforts expanded to catch novel resistance mechanisms. Although resistant bacteria were common previously only in intensive care units of hospitals, multidrug resistance has become an issue among strains of community-acquired pathogens such as Salmonella, Shigella, and even Neisseria gonorrhoeae (8, 9). To complicate matters even further, resistant organisms that arise in the community are now also spreading into healthcare settings (10, 11). Therefore, it is imperative that changes in resistance patterns of a wide range of bacterial pathogens be monitored continually to ensure optimal treatment both of the individual patients and for maintaining the effi cacy of empiric therapy regimens. This chapter will explore the methods used for antimicrobial susceptibility testing of bacterial pathogens.
Effects of Tube Diameter, Length and Tube Numbers on Condensation of Steam in Vertical Tube Condenser (2009)

A heat and mass analogy study was performed for the steam condensation in a vertical tube where steam is completely condensed. In the analysis a single tube, tube bundle with four tubes and two different diameter tubes were considered. The two condensing tubes of same height (0.9m) but different inside diameters, 26.6mm and 52.5mm, were used whereas the tube bundle was made of four tubes of height 1.8 m and 52.5 mm diameter. The results showed that the operating pressure is uniquely determined by inlet steam flow rate for the complete condensation. The condensation heat transfer rate increases and the condensation heat transfer coefficient decreases with the system pressure. The condensation heat transfer coefficients (HTC) were obtained as function various parameters such as different primary pressure (150–450 kPa) and inlet steam flow rate, single tube and tube bundle and tube diameters. Comparison with experimental condensation rates for single tube of 26.6 mm and 52.5 mm and four tube bundle of tube diameter 52.5 mm were made and the agreement was good. The effects of these parameters to condensation performance were evaluated.
An endoscopically implantable device stimulates the lower esophageal sphincter on demand by remote control: a study using a canine model (2007)

The LES pressure can be increased using an on-demand microstimulator. The implantation procedure is minimally invasive, represents a novel therapeutic approach to gastroesophageal reflux disease, and may have therapeutic potential for other gastrointestinal motility disorders.