gebhart/patrepeval huggingface dataset:
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells (2018)

The hybridization capture of chromatin-associated proteins for proteomics (HyCCAPP) technology was initially developed to uncover novel DNA-protein interactions in yeast. It allows analysis of a target region of interest without the need for prior knowledge about likely proteins bound to the target region. This, in theory, allows HyCCAPP to be used to analyze any genomic region of interest, and it provides sufficient flexibility to work in different cell systems. This method is not meant to study binding sites of known transcription factors, a task better suited for Chromatin Immunoprecipitation (ChIP) and ChIP-like methods. The strength of HyCCAPP lies in its ability to explore DNA regions for which there is limited or no knowledge about the proteins bound to it. It can also be a convenient method to avoid biases (present in ChIP-like methods) introduced by protein-based chromatin enrichment using antibodies. Potentially, HyCCAPP can be a powerful tool to uncover truly novel DNA-protein interactions. To date, the technology has been predominantly applied to yeast cells or to high copy repeat sequences in mammalian cells. In order to become the powerful tool we envision, HyCCAPP approaches need to be optimized to efficiently capture single-copy loci in mammalian cells. Here, we present our adaptation of the initial yeast HyCCAPP capture protocol to human cell lines, and show that single-copy chromatin regions can be efficiently isolated with this modified protocol.
Seasonal Variation of Essential Oil in Rosmarinus officinalis Leaves in Sardinia (2019)

Rosmarinus officinalis L. is an aromatic plant belonging to the Lamiaceae family widely distributed in the Mediterranean area. The interest on this species is related to the multiple uses of the plant as a food ingredient, in the pharmaceutical and cosmetic industries. The chemical composition of essential oil (EO) from 5 accessions of R. officinalis L., collected monthly through a full year in Sardinia, has been studied by gas chromatography (GC) and GC-mass spectrometry technique. The EO ranged from 0.29% to 0.89%. The qualitative determinations revealed the presence of 27 compounds belonging to 6 chemical groups (hydrocarbon monoterpene, alcohols, aldehydes, ketones, esters, hydrocarbon sesquiterpene). Overall the GC-flame ionization detector analysis showed the presence of 7 major compounds: α-pinene (26%-28%), camphene (5%-8%), 1,8-cineole (15%-25%), borneol (5%-11%), camphor (3%-12%), verbenone (6%-15%), and bornyl acetate (4%-7%). Chromatographic data were also subjected to a chemometric approach that evidenced discrimination of the samples according to the site of collection.
Properties of Sol-Gel Coating Film from Colloidal Silica-Silane Sol (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Development of a robotic FD‐CT‐guided navigation system for needle placement—preliminary accuracy tests (2011)

Robot absolute positioning accuracy was reduced to the navigation camera accuracy. The approach includes control strategies that may be very useful for interventional applications.
Prion Filament Networks in [Ure3] Cells of Saccharomyces cerevisiae (2001)

The [URE3] prion (infectious protein) of yeast is a self-propagating, altered form of Ure2p that cannot carry out its normal function in nitrogen regulation. Previous data have shown that Ure2p can form protease-resistant amyloid filaments in vitro, and that it is aggregated in cells carrying the [URE3] prion. Here we show by electron microscopy that [URE3] cells overexpressing Ure2p contain distinctive, filamentous networks in their cytoplasm, and demonstrate by immunolabeling that these networks contain Ure2p. In contrast, overexpressing wild-type cells show a variety of Ure2p distributions: usually, the protein is dispersed sparsely throughout the cytoplasm, although occasionally it is found in multiple small, focal aggregates. However, these distributions do not resemble the single, large networks seen in [URE3] cells, nor do the control cells exhibit cytoplasmic filaments. In [URE3] cell extracts, Ure2p is present in aggregates that are only partially solubilized by boiling in SDS and urea. In these aggregates, the NH(2)-terminal prion domain is inaccessible to antibodies, whereas the COOH-terminal nitrogen regulation domain is accessible. This finding is consistent with the proposal that the prion domains stack to form the filament backbone, which is surrounded by the COOH-terminal domains. These observations support and further specify the concept of the [URE3] prion as a self-propagating amyloid.
Bitmap indexes for relational XML twig query processing (2009)

Due to an increasing volume of XML data, it is considered prudent to store XML data on an industry-strength database system instead of relying on a domain specific application or a file system. For shredded XML data stored in the relational tables, however, it may not be straightforward to apply existing algorithms for twig query processing, because most of the algorithms require XML data to be accessed in a form of streams of elements grouped by their tags and sorted in a particular order. In order to support XML query processing within the common framework of relational database systems, we first propose several bitmap indexes for supporting holistic twig joins on XML data stored in the relational tables. Since bitmap indexes are well supported in most of the commercial and open-source database systems, the proposed bitmap indexes and twig query processing algorithms can be incorporated into the relational query processing framework with more ease. The proposed query processing algorithms are efficient in terms of both time and space, since the compressed bitmap indexes stay compressed during query processing. In addition, we propose a hybrid index which computes twig query solutions with only bit-vectors, without accessing labeled XML elements stored in the relational tables.
Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase (1994)

Enzymes with high specific activities at low temperatures have potential uses for chemical conversions when low temperatures are required, as in the food industry. Psychrotrophic microorganisms which grow at low temperatures may be a valuable source of cold-active enzymes that have higher activities at low temperatures than enzymes found for mesophilic microorganisms. To find cold-active beta-galactosidases, we isolated and characterized several psychrotrophic microorganisms. One isolate, B7, is an Arthrobacter strain which produces beta-galactosidase when grown in lactose minimal media. Extracts have a specific activity at 30 degrees C of 2 U/mg with o-nitrophenyl-beta-D-galactopyranoside as a substrate. Two isozymes were detected when extracts were subjected to electrophoresis in a nondenaturing polyacrylamide gel and stained for activity with 5-bromo-4-chloro-indolyl-beta-D-galactopyranoside (X-Gal). When chromosomal DNA was prepared and transformed into Escherichia coli, three different genes encoding beta-galactosidase activity were obtained. We have subcloned and sequenced one of these beta-galactosidase genes from the Arthrobacter isolate B7. On the basis of amino acid sequence alignment, the gene was found to have probable catalytic sites homologous to those from the E. coli lacZ gene. The gene encoded a protein of 1,016 amino acids with a predicted molecular mass of 111 kDa. The enzyme was purified and characterized. The beta-galactosidase from isolate B7 has kinetic properties similar to those of the E. coli lacZ beta-galactosidase but has a temperature optimum 20 degrees C lower than that of the E. coli enzyme.
Performance, performance system, and high performance system (2009)

This article proposes needed transitions in the field of human performance technology. The following three transitions are discussed: transitioning from training to performance, transitioning from performance to performance system, and transitioning from learning organization to high performance system. A proposed framework that comprises performance, performance system, and high performance system is suggested, along with recommendations for future research about the viability of this framework.
Optimization of Anti-kinking Designs for Vascular Grafts Based on Supramolecular Materials (2020)

Synthetic vascular grafts to be applied as access grafts for hemodialysis often require anti-kinking properties. Previously, electrospun microporous vascular implants based on synthetic supramolecular materials have been shown to perform adequately as resorbable grafts due to the microstructures, thereby enabling attraction of endogenous cells and consecutive matrix production in situ. Here, we use supramolecular materials based on hydrogen bonding interactions between bisurea (BU) or 2-ureido-4[1H]-pyrimidinones (UPy) to produce microporous anti-kinking tubular structures by combining solution electrospinning with 3D printing. A custom-made rational axis for 3D printing was developed to produce controlled tubular structures with freedom in design in order to print complex tubular architectures without supporting structures. Two different tubular grafts were developed, both composed of a three-layered design with a 3D printed spiral sandwiched in between luminal and adventitial electrospun layers. One tubular scaffold was composed of BU-polycarbonate electrospun layers with 3D printed polycaprolactone (PCL) strands in between for dimensional stability, and the other graft fully consisted of supramolecular polymers, using chain-extended UPy-PCL as electrospun layers and a bifunctional UPy-PCL for 3D printing. Both grafts, with a 3D printed spiral, demonstrated a reproducible dimensional stability and anti-kinking behavior under bending stresses.
Developments and applications in Electronic Sterilisation Technologies (2008)

ROLEST, The Robertson Trust Laboratory for Electronic Sterilisation Technologies, is a unique multidisciplinary research facility dedicated to the development and application of novel electrically based disinfection and sterilisation technologies. There is a growing need to control and prevent infection and this is a high priority in today's healthcare agenda. Against this background, ROLEST has been established to investigate and develop electro-technologies to combat public health problems and to provide solutions to difficult, and emerging, clinical challenges, as well as supporting the development of new medical technologies and procedures. Sterilisation technologies developed within ROLEST include Pulsed Electric Fields, Pulsed Ultraviolet Light, High-Intensity Narrow-Spectrum Light, and Advanced Oxidative Technologies, all of which demonstrate strong biocidal effects for use in a variety of applications. The successful development and application of these electrotechnologies by researchers at ROLEST aims to stimulate and enrich research into optimising innovative technologies to provide engineering-based solutions to complex scientific problems ranging from hospital-acquired infection to bio-terrorism.
Pleasant Stroke Touch on Human Back by a Human and a Robot (2021)

Abstract Pleasant touching is an important aspect of social interactions that is widely used as a caregiving technique. To address problems resulting from a lack of available human caregivers, previous research has attempted to develop robots that can perform this kind of pleasant touch. However, it remains unclear whether robots can provide such a pleasant touch in a manner similar to humans. To investigate this issue, we compared the effect of the speed of gentle strokes on the back between human and robot agents on the emotional responses of human participants (n = 28). A robot or a human stroked on the participants’ back at slow and medium speeds (i.e., 2.6 and 8.5 cm/s). Participants’ subjective (valence and arousal ratings) and physiological (facial electromyography (EMG) recorded from the corrugator supercilii and zygomatic major muscles, and skin conductance response) emotional reactions were measured. The subjective ratings demonstrated that the medium speed was more pleasant and arousing than the slow speed for both human and robot strokes. The corrugator supercilii EMG showed that the medium speed resulted in reduced activity in response to both human and robot strokes. These results demonstrate similar speed-dependent modulations of stroke on subjective and physiological positive emotional responses across human and robot agents and suggest that robots can provide a pleasant touch similar to that of humans.
Vector-field-smoothed Bayesian learning for incremental speaker adaptation (2002)

The paper presents a fast and incremental speaker adaptation method called MAP/VFS, which combines maximum a posteriori (MAP) estimation, or in other words Bayesian learning, with vector field smoothing (VFS). The point is that MAP is an intra-class training scheme while VFS is an inter-class smoothing technique. This is a basic technique for on-line adaptation which will be important in constructing a practical speech recognition system. Speaker adaptation speed of the incremental MAP is experimentally shown to be significantly accelerated by the use of VFS in word-by-word adaptation. The recognition performance of MAP is consistently improved and stabilized by VFS. The word error reduction rate achieved in incrementally adapting a few words of sample data is about 22%.
A network-centric terrain database regeneration architecture (2012)

A great deal has changed since 1997 when Schiavone first described the generic pipeline generation process for building an environmental database for military models and simulations. Environment representations have gone from cartoonist graphics to near real-life renderings possessing properties of physics and intelligence where appropriate. Within this article we discuss several contemporary and past terrain database generation processes, as well as challenges faced in generating terrain databases for military models and simulations today. These challenges include decentralized, distributed modification, frequent and incremental updates, and the need for a shared level of validity by users with heterogeneous needs. We propose a novel approach that addresses network-centric terrain database generation and provides distributed bi-directional, incremental updates that addresses many of these needs. We also developed, tested, and analyzed a prototype architecture and two types of feature modification applied to terrain within the network, the results of which are presented, along with conclusions and future research recommendations.
ANTIDIABETIC DRUGS IN AYURVEDA (2013)

Ayurveda the Indian traditional Medical science uses many drugs for diseases derived from medicinal plants, Minerals, herbo mineral. Diabetes (Madhumeha) is an important human ailment afflicting many from various walks of life in different countries. This review focuses on Ayurvedic drugs like plants, minerals in single or compound form in various research institutes and articles. A list of Ayurvedic drugs having antidiabetic and related beneficial in treatment of diabetes is compiled. These include, Trivanga Bhasma, Triphala Churna, Terminalia chebula, Nimbapatra, Ashvattha, Acacia arabica, Mangifera indica, Eugenia jambolana,
Synthesis of Spirotetramates via a Diels−Alder Approach (2011)

A study toward the unusual spirotetramate core of the pyrroindomycin antibiotics employing an intermolecular Diels-Alder reaction of an exo-methylene tetramic acid dienophile is described. The exo-methylene tetramate is readily synthesized from S-methylcysteine, and its reactivity as a dienophile is compared with that of related dehydroalanine derivatives. An alternative approach to spirotetramates using a nitroalkene dienophile is also reported.
Fabrication and characterization of electrospun polybutadiene fibers crosslinked by UV irradiation (2006)

Abstract Crosslinked electrospun polybutadiene (BR) fibers were made using electrospinning and UV curing methods. The crosslinked BR fibers were obtained by irradiating UV light on the electrospun BR fibers containing a photoinitiator and a crosslinker. Although uncrosslinked electrospun BR fibers did not retain the fiber morphology at room temperature due to a cold flow resulting from the very low glass transition temperature ( T g ) of BR (below −80°C), the crosslinked electrospun BR fibers retained the fiber morphology. The crosslink density increased with increase of the content of crosslinking agent. The crosslinked BR fibers had higher T g than the raw BR. Tensile strength, modulus, and elongation at break of the electrospun BR fiber mats increased with increase of the crosslinker content. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 101: 2233–2337, 2006
A Fuel Element for an Elevated-Temperature Critical Assembly (1959)

In its elevated-temperature critical-assembly experiments, the University of California Radiation Laboratory, Livermore, will utilize stainless-steel-clad fuel elements. The fuel element consists of 0.001- or 0.002-in. thick enriched-uranium foil packaged in a welded 0.002-in. thick type-347 stainless-steel envelope. The design requirements for the fuel elements are stated, as are the considerations which led to the design selected. Beta heat treatment of the uranium was found necessary to provide compatible thermal-expansion characteristics for the two materials in the fuel element.
Number Theory (2014)

We say an integer b evenly divides another integer c if c/b is a whole number. Actually, nobody in mathematics ever says that b “evenly divides” c – people just say b “divides” c. Another way to say the same thing is to say that b is a divisor of c. The divisors of c are the numbers that (evenly) divide c. Finally, one can also say that b is divisible by c.
Automated deep-phenotyping of the vertebrate brain (2017)

Here, we describe an automated platform suitable for large-scale deep-phenotyping of zebrafish mutant lines, which uses optical projection tomography to rapidly image brain-specific gene expression patterns in 3D at cellular resolution. Registration algorithms and correlation analysis are then used to compare 3D expression patterns, to automatically detect all statistically significant alterations in mutants, and to map them onto a brain atlas. Automated deep-phenotyping of a mutation in the master transcriptional regulator <i>fezf2</i> not only detects all known phenotypes but also uncovers important novel neural deficits that were overlooked in previous studies. In the telencephalon, we show for the first time that <i>fezf2</i> mutant zebrafish have significant patterning deficits, particularly in glutamatergic populations. Our findings reveal unexpected parallels between <i>fezf2</i> function in zebrafish and mice, where mutations cause deficits in glutamatergic neurons of the telencephalon-derived neocortex.
Associations of the metabolic syndrome and its components with cognitive impairment in older adults (2019)

The study was registered on 15th October 2014 at clinicaltrials.gov ( NCT02265263 ).
Soluble Polyimide Binder for Silicon Electrodes in Lithium Secondary Batteries (2015)

Abstract A solvent-soluble polyimide (PI) polymeric binder was synthesized by a two-step reaction for silicon (Si) anodes for lithium-ion batteries. Polyamic acid was first prepared through ring opening between two monomers, bicyclo[2,2,2]oct-7-ene-2,3,5,6-tetra-carboxylic dianhydride (BCDA) and 4,4-oxydianiline (ODA), followed by condensation reaction. Using the synthesized PI poly-meric binder (molecular weight = ~10,945), the coating slurry was then prepared and Si anode was fabricated. For the control system, Si anode based on polyvinylidene fluoride (PVDF, molecular weight = ~350,000) having the same constituent ratio was prepared. During precycling, PI polymeric binder revealed much improved discharge capacity (2,167 mAh g -1 ) compared to that of using PVDF polymeric binder (1,740 mAh g -1 ), while the Coulombic efficiency of two systems were similar. PI polymeric binder improved the cycle retention ability during cycles compared to that of using PVDF, which is attributed to an improved adhesion property inside Si anode diminishing the dimensional stress during Si volume changes. The adhesion property of each polymeric binder in Si anode was confirmed by surface and interfacial cutting analysis system (SAICAS) (Si anode based on PI polymeric binder = 0.217 kN m
Optimization of Multi-Cathode Membrane Electrolysers for the Indirect Electrochemical Reduction of Indigo (1998)

In order to color cellulose fibers with vat or sulfur dyes, large amounts of nonregenerable reducing agents are usually required. In this paper, the authors present the use of indirect electrochemical reduction, thus, offering economic and ecological advantages. Multi-cathode cells are used to overcome low cell current and a further increase in cell current is achieved by using three-dimensional flow-through electrodes.
Experimental Development of a Novel Stress Sensor for in situ Stress Measurement (2016)

This paper will describe ongoing work to adapt a previously demonstrated method for measuring stress in ceramics to develop a borehole deployed in situ stress sensor. The method involves the use of a cementitious material which exhibits a strong piezo-spectroscopic stress response as a downhole stress gage. A description of the conceptual approach will be provided along with preliminary analysis and proof-of-concept laboratory results.
Application of IL-2 and other cytokines in renal cancer (2004)

Renal cell carcinoma evokes an immune response, which investigators have attempted to augment by administering cytokines in doses above physiological levels. In 1992, high-dose (HD) bolus interleukin-2 (IL-2) received US Food and Drug Administration approval for metastatic renal cell carcinoma based on data that revealed durable responses in a small percentage of patients. However, this regimen is associated with significant toxicity and cost, which has limited its application to highly selected patients treated at specialised centres. Several investigators have evaluated regimens with lower doses of IL-2 in an attempt to decrease toxicity. Attempts were also made to improve treatment efficacy by adding interferon (IFN)-alpha followed by 5-fluorouracil to low-dose IL-2 regimens. These regimens were reported to produce response rates and survival comparable to HD IL-2 with much less toxicity, but possibly fewer durable responses. Based on positive preclinical data, other cytokines (e.g., IFN-gamma, IL-12) have also been given to patients with metastatic renal cell carcinoma with limited success. This review examines the clinical trials that have described the efficacy and toxicity of IL-2 and other cytokines in patients with renal cancer, with a particular focus on the Phase III trials that have helped to define the proper use of these agents.