gebhart/patrepeval huggingface dataset:
DNA Polymerase β:  Pre-Steady-State Kinetic Analyses of dATPαS Stereoselectivity and Alteration of the Stereoselectivity by Various Metal Ions and by Site-Directed Mutagenesis (2001)

The first pre-steady-state kinetic analysis of the stereoselectivity of a DNA polymerase, Pol beta from rat brain, toward Rp and Sp isomers of dATPalphaS, and alteration of the stereoselectivity by various metal ions and by site-directed mutagenesis are reported. Diastereomers of dATPalphaS were synthesized by enzymatic methods to >98% purity. The rate of polymerization (k(pol)) and the apparent dissociation constant (K(d,app)) were measured with dATP, Rp-dATPalphaS, and Sp-dATPalphaS in the presence of Mg(2+), Mn(2+), or Cd(2+). The results indicate that wild type (WT) polymerase (Pol) beta can incorporate both Sp- and Rp-dATPalphaS in the presence of Mg(2+), but Sp is the preferred isomer. The stereoselectivity, defined as (k(pol)/K(d))(Sp)/(k(pol)/K(d))(Rp) (abbreviated Sp/Rp ratio), is 57.5 in the presence of Mg(2+). When Mg(2+) was substituted with Mn(2+) and Cd(2+), the Sp/Rp ratio decreased to 7.6 and 21, respectively. These results are discussed in relation to the crystal structures of various Pol beta complexes, as well as previous steady-state kinetic studies of other DNA polymerases. In addition, the D276R mutant was designed to introduce a potential extra hydrogen bonding interaction between the arginine side chain and the pro-Sp oxygen of the alpha-phosphate of dNTP. The kinetic data of the D276R mutant showed a pronounced relaxation of stereoselectivity of dATPalphaS (Sp/Rp ratio = 1.5, 3.7, and 1.5 for Mg(2+), Mn(2+), and Cd(2+), respectively). Furthermore, the D276R mutant showed a 5-fold enhanced reactivity toward Rp-dATPalphaS relative to WT Pol beta, suggesting that this mutant Pol beta can be used to incorporate Rp-dNTPalphaS into DNA oligomers.
Drug policy and services : an overview / Department of Human Services (2004)

Changing drug use and drug injecting patterns pose significant challenges for alcohol and drug treatment service systems and policy makers. Persistent trends in harmful licit and illicit drug use often require new and strategic policy directions. Clientspresenting with multiple, problematic drug and other health issues need a flexible, integrated and highly responsive service system, and a skilled and experienced workforce. The Victorian Government, in partnership with the community and other bodies, has employed a holistic approach to dealing with problematic drug use at an individual and community level. The Victorian Department of Human Services Drug Policy and Services program manages activities that aim to promote and protect the health and wellbeing of all Victorians by reducing death, disease and social harm caused by the use and misuse of licit and illicit drugs. The Drugs Policy and Services Branch is responsible for strategic leadership in policy and service development, funding and implementing program initiatives and regulating and monitoring safety standards. (Foreword, introduction, edited.)
Annular focusing lens based on transformation optics (2014)

We propose and design a kind of annular focusing lens based on transformation optics. Based on the method of eigen-mode expansions, closed-form expressions are derived to analyze the proposed annular lens rigorously. We show that the annular lens has excellent focusing property. Even when a barrier (such as a conducting cylinder) exists in the center of the lens, the analytical results demonstrate that the waves are still guided to propagate smoothly and focused on a spot.
Characteristics of Milk and Butter from Cows Fed Sunflower Seeds (1988)

Composition of milk and butter was evaluated from cows fed either control or experimental diets containing added fat of partially unsaturated fatty acid composition. The control diet concentrate mix consisted mainly of corn and soybean meal. The two experimental diets substituted either 20% high oleic sunflower seeds (>79% of fatty acids as oleic acid) or 20% regular sunflower seeds (>67% of fatty acids as linoleic acid) for part of the corn and soybean meal in the concentrate mix. Feeding lactating dairy cattle sunflower seeds resulted in lower concentrations of short and medium chain and higher concentrations of long-chain fatty acids in milk fat and butter. Milk unsaturated fatty acid concentrations were (28.9, 38.8, 45.6%), and butter unsaturated fatty acid concentrations were (29.6, 38.1, 44.3%) for control, high oleic sunflower seed, and regular sunflower seed treatment milk, respectively. Organoleptic evaluation indicated the high oleic sunflower seed and regular sunflower seed treatment butters were equal or superior in flavor to the control butter. The high oleic sunflower seed and regular sunflower seed treatment butters were softer, more unsaturated, and exhibited acceptable flavor, manufacturing, and storage characteristics.
Swelling Behavior of Carboxymethylcellulose Hydrogels in Relation to Cross-Linking, pH, and Charge Density (2000)

Hydrogels of carboxymethylcellulose (CMC) with 2.5%, 25%, 50%, and 100% cross-linking were prepared. Cross-linking and stoichiometry was controlled by appropriate addition of CMPJ and determined by potentiometric titration. The cross-linked polymer was then sulfated using a sulfur trioxide−pyridine complex (SO3−Py) in a heterogeneous mixture. Water uptake of the gels was studied in relation to cross-linking, pH, and sulfation. FT-IR spectra of the gels were recorded at different pHs to evidence the hydrogen bonds. Hydrogen bonds formation between the chains may explain the correlation found between water uptake properties and the chemical composition of the gels.
Selective activation of PPARα maintains thermogenic capacity of beige adipocytes (2023)

Beige adipocytes are inducible thermogenic adipocytes used for anti-obesity treatment. Beige adipocytes rapidly lose their thermogenic capacity once external cues are removed. However, long-term administration of stimulants, such as PPARγ and β-adrenergic receptor agonists, is unsuitable due to various side effects. Here, we reported that PPARα pharmacological activation was the preferred target for maintaining induced beige adipocytes. Pemafibrate used in clinical practice for dyslipidemia was developed as a selective PPARα modulator (SPPARMα). Pemafibrate administration regulated the thermogenic capacity of induced beige adipocytes, repressed body weight gain, and ameliorated impaired glucose tolerance in diet-induced obese mouse models. The transcriptome analysis revealed that the E-twenty-six transcription factor ELK1 acted as a cofactor of PPARα. ELK1 was mobilized to the <i>Ucp1</i> transcription regulatory region with PPARα and modulated its expression by pemafibrate. These results suggest that selective activation of PPARα by pemafibrate is advantageous to maintain the function of beige adipocytes.
The double mechanism of incompatibility between λ plasmids and Escherichia coli dnaA(ts) host cells (2001)

For plasmids derived from bacteriophage lambda, the initiation of bidirectional DNA replication from orilambda depends on the stimulation of transcription from the p(R) promoter by the host replication initiator protein DnaA. Certain Escherichia coli dnaA(ts) mutants cannot be transformed by wild-type lambda plasmids even at the temperature permissive to cell growth. This plasmid-host incompatibility appeared to be due to inefficient stimulation of transcription from the p(R) promoter by the mutant DnaA protein. This paper shows that there is a second mechanism for the incompatibility between lambda plasmids and dnaA(ts) hosts, exemplified in this study by the dnaA46 mutant. This is based on the competition between the lambda P protein and the host DnaA and DnaC proteins for DnaB helicase. Both mechanisms must be operative for the incompatibility.
Room Decontamination with UV Radiation (2010)

This UV-C device was effective in eliminating vegetative bacteria on contaminated surfaces both in the line of sight and behind objects within approximately 15 minutes and in eliminating C. difficile spores within 50 minutes.
Three Software Tools for Viewing Sectional Planes, Volume Models, and Surface Models of a Cadaver Hand (2018)

These new tools involving the realistic images of a cadaver and the diverse functions are expected to improve comprehensive knowledge of the hand shape.
Symptomatic lymphoceles after kidney transplantation – multivariate analysis of risk factors and outcome after laparoscopic fenestration (2009)

Lymphocele formation is a common complication after kidney transplantation, and laparoscopic surgery has become a widely accepted treatment option. The aim of this retrospective study was to analyze the risk factors of lymphocele development and to assess the treatment outcome after laparoscopic fenestration. We analyzed 426 renal allograft recipients operated between 2002 and 2006 receiving triple immunosuppression with calcineurin inhibitors. The incidence of lymphocele was 9.9%, while 24 (5.6%) patients with symptomatic lymphoceles required laparoscopic surgery. Serum creatinine at diagnosis was significantly higher in patients with lymphoceles treated surgically (3.2 +/- 0.7 vs. 1.7 +/- 0.6 mg/dL; p < 0.001). After successful laparoscopic intervention, creatinine concentrations recovered until discharge and were comparable to other patients (1.6 +/- 0.5 vs. 1.5 +/- 0.5 mg/dL; p = NS). While we observed a significant association of lymphocele formation with diabetes, tacrolimus therapy, and acute rejection in univariate testing, only diabetes remained a significant factor after multivariate analysis. Laparoscopic fenestration proved to be a safe and efficient method without any associated mortality and a low recurrence rate of 8.3% (n = 2). We conclude that diabetes is an independent risk factor for lymphocele development, and laparoscopic fenestration should be the treatment of choice for larger and symptomatic lymphoceles, as it is safe and offers a low recurrence rate.
Clinical Trial of the Protein Farnesylation Inhibitors Lonafarnib, Pravastatin, and Zoledronic Acid in Children With Hutchinson-Gilford Progeria Syndrome (2016)

URL: http://www.clinicaltrials.gov. Unique identifiers: NCT00879034 and NCT00916747.
Two-component, small molecule gelling agents (1993)

A 1 : 1 mixture of 5-hexadecyl-2,4,6-triaminopyrimidine and 5,5-didodecylbarbituric acid can cause physical gelation in organic liquids; the gel phenomenon is investigated by FTIR spectroscopy, TEM (transmission electron microscopy) and X-ray diffraction.
Enzymatic RNA synthesis in self‐reproducing vesicles: An approach to the construction of a minimal synthetic cell (1994)

Abstract Our approach towards “core‐and‐shell self‐reproduction” is reviewed here. With this term, we indicate a process by which the shell‐reproduction of a spherically bounded system (micelles or vesicles) proceeds simultaneously with the replication of nucleic acid which is hosted inside the micelles or vesicles. The realization of such a process is seen as a step towards the construction of a synthetic cell model. Two chemical systems are examined here, one based on the polynucleotide phosphorylase‐catalyzed synthesis of poly(A) starting from ADP; and the other based on the enzyme Q β replicase, which is able to catalyze the synthesis of a RNA template. In both cases, production of RNA macromolecules proceeds simultaneously with self‐reproduction of the oleic acid/oleate vesicles. One still open question is the determination of the redistribution of the guest macromolecular components during the reproduction of the shell. Within these limits, it is argued that this work offers a system which goes beyond the two approaches to self‐replication presented until now in the literature, namely the template self‐reproduction of linear sequences of oligonucleotides and the autopoietic shell reproduction of micelles and vesicles.
The Role of the 11‐cis‐Retinal Ring Methyl Substituents in Visual Pigment Formation (2006)

Artificial visual pigment formation from ring-demethylated retinals was studied in an effort to understand the effect that methyl groups on the chromophore cyclohexenyl ring have on the visual cycle. The stereoselective synthesis of the 11-cis-ring-demethylated analogues involves thallium-accelerated Suzuki cross-coupling reactions and highly stereocontrolled Wittig reactions to form key bonds. Only 11-cis-1,1,5-trisdemethylretinal (2) failed to form an artificial pigment, whilst variable pigment-formation yields were determined for the remaining analogues, increasing with the number (and location) of the chromophore hydrophobic ring methyl groups. Our results with the monodemethylated analogues 11-cis-5-demethylretinal (4) and 11-cis-1-demethylretinal (5) show that the C1-2-CH(3) groups are more important for pigment formation than the C5-CH(3) substituent. This is reflected in the absorption maxima of the artificial pigments, with values closer to that of native rhodopsin for 4. Docking studies based on a rhodopsin crystal structure, however, predict a lower pigment stability for 4 than for 5. Gas-phase DFT (B3LYP/6-31G*) computations of the free-ligand geometries, conformational searches about the C6--C7 bond, and docking studies revealed that, although the conformation of bound 5 is close to that of the native chromophore, the ligand needs to overcome the energy cost of shifting the unbound favored 6-s-trans conformation to the bound 6-s-cis form. In addition, the presence of an extra methyl group at C18 (11-cis-18-methylretinal, 7) is tolerated well and adds further stability to the complex, most probably due to increased hydrophobic interactions.
Performing optimized collective operations in a irregular subcommunicator of compute nodes in a parallel computer (2023)

In a parallel computer, performing optimized collective operations in an irregular subcommunicator of compute nodes may be carried out by: identifying, within the irregular subcommunicator, regular neighborhoods of compute nodes; selecting, for each neighborhood from the compute nodes of the neighborhood, a local root node; assigning each local root node to a node of a neighborhood-wide tree topology; mapping, for each neighborhood, the compute nodes of the neighborhood to a local tree topology having, at its root, the local root node of the neighborhood; and performing a one way, rooted collective operation within the subcommunicator including: performing, in one phase, the collective operation within each neighborhood; and performing, in another phase, the collective operation amongst the local root nodes.
Breeding and Characteristics of Flue-cured Tobacco cv. Yunyan105 (2013)

In order to meet the need of tobacco production for quality varieties, Yunyan105 was bred with Yunyan87 and CF965 as female and male parents respectively. Yunyan105 grew vigorously in field with a tower-shaped plant and long oval leaves. The average height of topped plants was 117.5 cm with 22 harvestable leaves and the growth duration in field was 128 days. The plant was moderately resistant to black shank and brown spot disease, and its comprehensive resistance was comparable to K326. The major indexes of economic characters of Yunyan105 were better than K326. The main chemical composition of Yunyan105 was harmonious, its appearance quality of cured leaves was slightly better than or similar to K326, and sensory quality was moderate or above, which was comparable to K326. Yunyan105 is suitable for planting in tobacco growing areas in south China.
New technology, new complications: pancreatitis complicating double-balloon enteroscopy. (2007)

Double-balloon enteroscopy (DBE) is a novel endoscopic technique that allows for visualization of the entire small bowel and has the utility to perform biopsies and treat suitable lesions. Complications common to any endoscopic procedure such as bleeding or perforation can occur with this technique and should be discussed with the patient. We report a case of acute pancreatitis following DBE and review the literature regarding this rare complication.
Bioelectric Control of a 757 Class High Fidelity Aircraft Simulation (2000)

This paper presents results of a recent experiment in fine grain Electromyographic (EMG) signal recognition. We demonstrate bioelectric flight control of 757 class simulation aircraft landing at San Francisco International Airport. The physical instrumentality of a pilot control stick is not used. A pilot closes a fist in empty air and performs control movements which are captured by a dry electrode array on the arm, analyzed and routed through a flight director permitting full pilot outer loop control of the simulation. A Vision Dome immersive display is used to create a VR world for the aircraft body mechanics and flight changes to pilot movements. Inner loop surfaces and differential aircraft thrust is controlled using a hybrid neural network architecture that combines a damage adaptive controller (Jorgensen 1998, Totah 1998) with a propulsion only based control system (Bull &amp;amp; Kaneshige 1997). Thus the 757 aircraft is not only being flown bioelectrically at the pilot level but also demonstrates damage adaptive neural network control permitting adaptation to severe changes in the physical flight characteristics of the aircraft at the inner loop level. To compensate for accident scenarios, the aircraft uses remaining control surface authority and differential thrust from the engines. To the best of our knowledge this is the first time real time bioelectric fine-grained control, differential thrust based control, and neural network damage adaptive control have been integrated into a single flight demonstration. The paper describes the EMG pattern recognition system and the bioelectric pattern recognition methodology.
SEROTONERGIC MECHANISMS OF ECT: NEUROENDOCRINE EVIDENCE (1992)

*Depression Treatment Unit, Herzog Hospital; **Dept. of Psychiatry, Hadassah-Hebrew University Medical Center, Jerusalem, Israel
The Impact of a One-Dose versus Two-Dose Oral Cholera Vaccine Regimen in Outbreak Settings: A Modeling Study (2015)

Reactive vaccination campaigns using a single dose of OCV may avert more cases and deaths than a standard two-dose campaign when vaccine supplies are limited, while at the same time reducing logistical complexity. These findings should motivate consideration of the trade-offs between one- and two-dose campaigns in resource-constrained settings, though further field efficacy data are needed and should be a priority in any one-dose campaign.
The Effect of Different Text Presentation Formats on Eye Movement Metrics in Reading (2012)

Eye movement data were collected and analyzed from 16 participants while they read text from a computer screen. Several text presentation formats were compared, including sentences as part of a full paragraph, sentences presented one by one, sentences presented in chunks of at most 30 characters at a predefined rate, and line-by-line presentation fitting the width of the computer screen. The goal of the experiment was to study how these different text presentation modes affect eye movement metrics (fixation duration, fixations per minute, regressions, etc.). One-way repeated measures ANOVA revealed that differences in presentation format have a significant effect on fixation duration, number of fixations per minute, and number of regressions.
Fate of Grease in Sludge Digestion (1927)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFate of Grease in Sludge DigestionS. L. Neave and A. M. BuswellCite this: Ind. Eng. Chem. 1927, 19, 9, 1012–1014Publication Date (Print):September 1, 1927Publication History Published online1 May 2002Published inissue 1 September 1927https://pubs.acs.org/doi/10.1021/ie50213a019https://doi.org/10.1021/ie50213a019research-articleACS PublicationsRequest reuse permissionsArticle Views43Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Sound Source Localization Based on von-Mises-Bernoulli Deep Neural Network (2020)

This paper addresses the learning of periodic information such as the phase for deep neural networks (DNN). To solve this problem, we propose a novel activation function based on the von Mises distribution used in directional statistics, and we construct von-Mises-Bernoulli DNN by replacing the activation function in the first hidden layer of the conventional Bernoulli-Bernoulli DNN with the proposed activation function. We theoretically validate that this simple change can handle periodic information by using restricted Boltzmann machines. In addition, we practically show that the constructed von-Mises-Bernoulli DNN can learn periodic phase information through numerical simulation for sound source localization. Experimental results showed that sound source localization accuracy was almost 100% for 5° resolution, and 60% for 1° resolution under noise condition of 20 dB signal-to-noise ratio.
Biological and Biochemical Characteristics of Cytoadhesion of Plasmodium falciparum -Infected Erythrocytes to Chondroitin-4-Sulfate (1998)

The cytoadhesion of Plasmodium falciparum laboratory strains and clones to Saimiri brain microvascular endothelial cells (SBEC 17), with chondroitin-4-sulfate (CSA) as the only adhesion receptor, was tested. Only one strain had significant cytoadhesion. However, CSA-specific infected erythrocytes (IRBCs) were detected in all strains after selection of a CSA-specific subpopulation by culturing the few adherent IRBCs. This demonstrates the lack of sensitivity of cytoadhesion microassays for detecting small quantities of CSA-specific IRBCs in cultures or field isolates. Cytoadhesion to CSA is maximal at 24 h of the cycle and decreases with the onset of schizogony, reaching a minimum just before reinvasion. This fluctuation must be taken into account in comparisons of the cytoadhesion of different strains or isolates. The minimum size of CSA for active inhibition was 4 kDa, and a mass of 9 kDa was required for inhibition similar to that obtained with the 50-kDa CSA. In contrast to cytoadhesion to CSA, which is pH independent or maximal at physiological pH (depending on the target endothelial cells), adhesion to CD36 and intercellular adhesion molecule 1 was pH dependent, requiring acidic conditions to be maximal in all cases. Cytoadhesion to CSA may trigger the occlusion of microvessels and cause the acidosis necessary for the other receptors to be fully efficient. If this key role in the mechanisms of sequestration were to be confirmed in vivo, prevalence studies of the CSA cytoadhesion phenotype would have to be reevaluated, because simple cytoadhesion assays do not detect CSA-specific parasites present in very low numbers, and these parasites might then be undetected in the peripheral blood but present in organs in which sequestration occurs, such as the placenta (M. Fried and P. E. Duffy, Science 272:1502-1504, 1996).