gebhart/patrepeval huggingface dataset:
Kinetic and Mechanistic Analysis of theE. colipanE-Encoded Ketopantoate Reductase (2000)

Ketopantoate reductase (EC 1.1.1.169) catalyzes the NADPH-dependent reduction of alpha-ketopantoate to form D-(-)-pantoate in the pantothenate/coenzyme A biosynthetic pathway. The enzyme encoded by the panE gene from E. coli K12 was overexpressed and purified to homogeneity. The native enzyme exists in solution as a monomer with a molecular mass of 34 000 Da. The steady-state initial velocity and product inhibition patterns are consistent with an ordered sequential kinetic mechanism in which NADPH binding is followed by ketopantoate binding, and pantoate release precedes NADP(+) release. The pH dependence of the kinetic parameters V and V/K for substrates in both the forward and reverse reactions suggests the involvement of a single general acid/base in the catalytic mechanism. An enzyme group exhibiting a pK value of 8.4 +/- 0.2 functions as a general acid in the direction of the ketopantoate reduction, while an enzyme group exhibiting a pK value of 7.8 +/- 0.2 serves as a general base in the direction of pantoate oxidation. The stereospecific transfer of the pro-S hydrogen atom of NADPH to the C-2 position of ketopantoate was demonstrated by (1)H NMR spectroscopy. Primary deuterium kinetic isotope effects of 1.3 and 1.5 on V(for) and V/K(NADPH), respectively, and 2.1 and 1.3 on V(rev) and V/K(HP), respectively, suggest that hydride transfer is not rate-limiting in catalysis. Solvent kinetic isotope effects of 1.3 on both V(for) and V/K(KP), and 1.4 and 1.5 on V(rev) and V/K(HP), respectively, support this conclusion. The apparent equilibrium constant, K(eq)', of 676 at pH 7.5 and the standard free energy change, DeltaG, of -14 kcal/mol suggest that ketopantoate reductase reaction is very favorable in the physiologically important direction of pantoate formation.
How do older persons living with dementia journey through the long-term care system? (2020)

Many older persons living with dementia and their families must rely on the long-term care (LTC) system. This research looks at how older persons with dementia journey through the LTC system, and what predicts their movement from home care (HC) into facility-based residential care (RC), as well as their unique experiences as they journey through the LTC system.
Risk of Influenza A (H5N1) Infection among Poultry Workers, Hong Kong, 1997–1998 (2002)

In 1997, outbreaks of highly pathogenic influenza A (H5N1) among poultry coincided with 18 documented human cases of H5N1 illness. Although exposure to live poultry was associated with human illness, no cases were documented among poultry workers (PWs). To evaluate the potential for avian-to-human transmission of H5N1, a cohort study was conducted among 293 Hong Kong government workers (GWs) who participated in a poultry culling operation and among 1525 PWs. Paired serum samples collected from GWs and single serum samples collected from PWs were considered to be anti-H5 antibody positive if they were positive by both microneutralization and Western blot testing. Among GWs, 3% were seropositive, and 1 seroconversion was documented. Among PWs, approximately 10% had anti-H5 antibody. More-intensive poultry exposure, such as butchering and exposure to ill poultry, was associated with having anti-H5 antibody. These findings suggest an increased risk for avian influenza infection from occupational exposure.
Study of burst-mode adaptive equalization for >25G PON applications [Invited] (2019)

In anticipation of emerging applications such as business services, 5G, and the Internet of Things, research and standardization are working together towards next-generation 25/50 Gbit/s passive optical network access networks. However, the increase in single-channel line rate is limited by the distortion introduced by the low-cost device’s bandwidth limit or the chromatic dispersion in the fiber. Burst-mode electronic distortion/dispersion compensation can be used to mitigate these effects, but requires a burst-mode equalizer (BMEQ) for upstream traffic and introduces many challenges. In this invited paper, the feasibility of single-channel, directly modulated upstream 25 Gbit/s non-return-to-zero transmission is investigated in more detail. By using a linear burst-mode transimpedance amplifier together with an offline BMEQ, specific BMEQ penalties and trade-offs such as limited training time and burst-to-burst phase variation are considered.
A Supplier's Optimal Quantity Discount Policy Under Asymmetric Information (2000)

In the supply-chain literature, an increasing body of work studies how suppliers can use incentive schemes such as quantity discounts to influence buyers' ordering behaviour, thus reducing the supplier's (and the total supply chain's) costs. Various functional forms for such incentive schemes have been proposed, but a critical assumption always made is that the supplier has full information about the buyer's cost structure. We derive the optimal quantity discount policy under asymmetric information and compare it to the situation where the supplier has full information.
Opportunities for customer segmentation strategies (1994)

Customer segmentation has recently become an invaluable tool for marketers in Japan. Evolving market and customer needs are transforming the country's retail and service paradigm.
Automatic 3D facial expression recognition based on polytypic Local Binary Pattern (2014)

Most of the existing methods adopted in 3D facial expression recognition require manual-intervention, which should be avoided in automatic systems. In this paper, we propose an automatic data normalization method based on the point-to-point mapping between the depth value and the texture information of the facial data. Based on this, we further construct a new kind of 3D facial expression feature, namely polytypic Local Binary Patterns (p-LBP), which involves both the irregular divisions to keep the integrity of local faces and the fusion of depth and texture information of 3D models to enhance the recognition accuracy. The proposed strategy is tested on BU-3DFE database, and three kinds of classifiers are employed. Their remarkable results outperform the state of the art, and show the effectiveness of proposed features for 3D facial expression recognition. Therefore, p-LBP is validated and its simplicity opens a promising direction for automatic 3D facial expression recognition.
Optimizing the diagnostic power with gastric emptying scintigraphy at multiple time points (2011)

With a mixed group of patients either referred with suspected gastroparesis or investigated for other reasons, the CART model is more robust than the LDA and DF approaches, capable of accommodating covariate effects and can be generalized for cross institutional applications, but could be unstable if sample size is limited.
Diffusion of sulfuric acid in protective organic coatings (2017)

Organic protective coatings and linings are widely used to prevent corrosion in industrial processes. However, the permeation of aggressive chemicals through coatings can induce failures. These are described in the literature, but rarely quantified. To measure the diffusion rates of aggressive chemicals through organic coatings, a diffusion cell was designed. The diffusion cell designed is simple, small and suitable for scaling up to a battery of cells. A concentration profile of H3O+ through epoxy coatings was achieved for sulfuric acid aqueous solutions, which can be used to estimate diffusion coefficients of H3O+. Typically, Crank’s time-lag method is used to estimate the diffusion coefficients but more complicated modelling is required for sulfuric acid solutions. Future modelling work is needed to estimate diffusion behavior of sulfuric acid.
PrimeArray: genome-scale primer design for DNA-microarray construction (2001)

PrimeArray is a Windows program that computes oligonuceotide primer pairs for genome-scale gene amplification by the Polymerase Chain Reaction (PCR). The program supports the automated extraction of coding sequences (CDS) from various input-file formats and allows highly automated primer pair-optimization.
Mechanism for acute reduction of 1,5-anhydroglucitol in rats treated with diabetogenic agents (1990)

The mechanism for acute reduction of plasma 1,5-anhydroglucitol (AG) in experimental diabetic rats was studied. Acute AG decrease was induced not only by diabetogenic agents, such as streptozotocin (STZ) and alloxan, but also by phloridzin, which caused glucosuria but not hyperglycemia. A similar reduction also occurred in hyperglycemia induced by glucose injection. The AG reduction induced by STZ was completely abolished by bilateral nephrectomy or by euglycemia with insulin treatment. The decrease of plasma AG was well correlated with the degree of urinary excretion of AG, which in turn reflected the degree of urinary glucose excretion, irrespective of the kind of agent causing the glucosuria. Under conditions of continuous glucose infusion, AG concentration decreased not only in plasma but also in various tissues and organs. The amount of AG lost was estimated to be almost equal to that excreted into urine during the period of infusion. These observations suggest that the degree of reduction of plasma AG depends simply on the urinary excretion of glucose, and it was assumed that the urinary excretion of 0.5 mg AG corresponds to the urinary excretion of 100 mg glucose during a short period after the onset of glucosuria.
Extensible security architectures for Java (1997)

article Extensible security architectures for Java Share on Authors: Dan S. Wallach Department of Computer Science, Princeton University Department of Computer Science, Princeton UniversityView Profile , Dirk Balfanz Department of Computer Science, Princeton University Department of Computer Science, Princeton UniversityView Profile , Drew Dean Department of Computer Science, Princeton University Department of Computer Science, Princeton UniversityView Profile , Edward W. Felten Department of Computer Science, Princeton University Department of Computer Science, Princeton UniversityView Profile Authors Info & Claims ACM SIGOPS Operating Systems ReviewVolume 31Issue 5Dec. 1997 pp 116–128https://doi.org/10.1145/269005.266668Online:01 October 1997Publication History 141citation1,660DownloadsMetricsTotal Citations141Total Downloads1,660Last 12 Months11Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Tri-state delta modulation system for Space Shuttle digital TV downlink (1981)

Future requirements for Shuttle Orbiter downlink communication may include transmission of digital video which, in addition to black and white, may also be either field-sequential or NTSC color format. The use of digitized video could provide for picture privacy at the expense of additional onboard hardware, together with an increased bandwidth due to the digitization process. A general objective for the Space Shuttle application is to develop a digitization technique that is compatible with data rates in the 20-30 Mbps range but still provides good quality pictures. This paper describes a tri-state delta modulation/demodulation (TSDM) technique which is a good compromise between implementation complexity and performance. The unique feature of TSDM is that it provides for efficient run-length encoding of constant-intensity segments of a TV picture. Axiomatix has developed a hardware implementation of a high-speed TSDM transmitter and receiver for black-and-white TV and field-sequential color. The hardware complexity of this TSDM implementation is summarized in the paper.
Molecular Phylogeny of the Animal Kingdom (1988)

A rapid sequencing method for ribosomal RNA was applied to the resolution of evolutionary relationships among Metazoa. Representatives of 22 classes in 10 animal phyla were used to infer phylogenetic relationships, based on evolutionary distances determined from pairwise comparisons of the 18S ribosomal RNA sequences. The classical Eumetazoa are divided into two groups. Cnidarians arose from a protist ancestry different from the second group, the Bilateria. Within the Bilateria, an early split gave rise to Platyhelminthes (flatworms) and the coelomate lineage. Coelomates are thus monophyletic, and they radiated rapidly into four groups: chordates, echinoderms, arthropods, and eucoelomate protostomes.
A 400-MHz input flash converter with error correction (1990)

An 8-b, 200-megasample/s flash converter with 400-MHz analog bandwidth and error correction circuitry is described. A cascoded input stage and a dense bipolar process make the wide bandwidth possible. Errors arising from high input slew rate and comparator metastability are reduced by means of the circuitry and the latching stages respectively. The final defense against errors is the second rank error suppression. Measured frequency and error rate performance are examined.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Control of Silver(I)–Dialkyl Chalcogenide Coordination by a Synthetic Cavity (2014)

Abstract The discrete cavity of a self‐assembled palladium–tris(4‐pyridyl)triazine cage dictates the ratio of metal, ligand, and a non‐coordinative molecule in the formation of silver(I)–dialkyl chalcogenide (Et, n Bu; S, Se) complexes and defines their coordination arrangement.
Sulforaphane Inhibits Lipopolysaccharide-Induced Inflammation, Cytotoxicity, Oxidative Stress, and miR-155 Expression and Switches to Mox Phenotype through Activating Extracellular Signal-Regulated Kinase 1/2–Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Response Element Pathway in Murine Microglial Cells (2018)

Sulforaphane (SFN) is a natural product with cytoprotective, anti-inflammatory, and antioxidant effects. In this study, we evaluated the mechanisms of its effects on lipopolysaccharide (LPS)-induced cell death, inflammation, oxidative stress, and polarization in murine microglia. We found that SFN protects N9 microglial cells upon LPS-induced cell death and suppresses LPS-induced levels of secreted pro-inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. SFN is also a potent inducer of redox sensitive transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), which is responsible for the transcription of antioxidant, cytoprotective, and anti-inflammatory genes. SFN induced translocation of Nrf2 to the nucleus <i>via</i> extracellular signal-regulated kinase 1/2 (ERK1/2) pathway activation. siRNA-mediated knockdown study showed that the effects of SFN on LPS-induced reactive oxygen species, reactive nitrogen species, and pro-inflammatory cytokine production and cell death are partly Nrf2 dependent. Mox phenotype is a novel microglial phenotype that has roles in oxidative stress responses. Our results suggested that SFN induced the Mox phenotype in murine microglia through Nrf2 pathway. SFN also alleviated LPS-induced expression of inflammatory microRNA, miR-155. Finally, SFN inhibits microglia-mediated neurotoxicity as demonstrated by conditioned medium and co-culture experiments. In conclusion, SFN exerts protective effects on microglia and modulates the microglial activation state.
TCAD Simulation Modeling of Mold Epoxy Resin Applied for Encapsulation of Power Devices (2023)

The impact of carrier injection into mold epoxy resin on the device leakage current of power modules is investigated by our proposed model of the resin and its application to a simplified power module. Charge redistribution or polarization of the resin alters the surface potential of the edge termination region, leading to electric failure during reliability tests such as High- Temperature Reverse Bias test. While attempts to simulate such behavior have been reported, the modeling of the epoxy resin and its interaction with the device termination and bonding wires are not fully understood. This paper shed light on this issue by presenting a physically reasonable assumption that can help resolve it.
5,13-Diethyl-10-methyl-8-heptadecanone: A component of post-1976 Kelex 100 (1991)

The title compound was prepared by mixed aldol condensation of 2-ethylhexanal and acetone, double bond hydrogenation, aldol autocondensation of the resulting saturated ketone and finel double bond hydrogenation. It is identical with the ketone C 22 H 44 O previously isolated from new Kelex 100 which was erroneously assigned a furoquinoline structure.
Demonstration of newly invented negative-mask scanning imaging scheme using THz-radiation sources (2003)

Summary form only given. There have been strong demands for a new imaging scheme with limited light source intensity for various imaging applications. Additionally, there is no detector array with sufficient sensitivity or spatial resolution for some radiation. Therefore, scanning imaging schemes with a single detector will be required. If the object is not in an easy position to scan, a mask position scanning scheme will be desirable to enable a compact imaging system to be constructed. In such a technique, the simplest mask is a pin-hole mask. This scheme is not practical, due to the significant reduction of signal intensity with a pin-hole mask required for high spatial resolution. In this presentation, we have reported a newly invented scanning imaging scheme to solve these problems and have demonstrated it with a THz-radiation light source.
Projections for Solid Oxide Electrolysers for Water Electrolysis (2021)

It is shown that lowering area specific resistance (ASR) or R t (total resistance) should lead to lower capital cost, CAPEX, and also to the lowest possible electrical power consumption per hydrogen produced, OPEX. Many researchers have focused for many years on thinning component layers and developing new higher-performance materials. Thinning of components to lower RT is widely researched. Based on simple correlations, the CAPEX of the solid oxide electrolysis cell (SOEC) stack cost could expect to be lowered by 25 percent by thinning components. In terms of size this low- R t SOEC stack has the potential to be significantly smaller. Building off the DOE’s progress with SOFC, even lower R t SOEC and SOFC’s are possible with techniques such as sputtering and Atomic Layer Deposition (ALD) which produce better conducting materials. This is truly remarkable in that the low per kilowatt SOEC stack cost and low power consumption may make the SOEC a truly realizable economically viable SO manifestation.
Robust online belief space planning in changing environments: Application to physical mobile robots (2014)

Motion planning in belief space (under motion and sensing uncertainty) is a challenging problem due to the computational intractability of its exact solution. The Feedback-based Information RoadMap (FIRM) framework made an important theoretical step toward enabling roadmap-based planning in belief space and provided a computationally tractable version of belief space planning. However, there are still challenges in applying belief space planners to physical systems, such as the discrepancy between computational models and real physical models. In this paper, we propose a dynamic replanning scheme in belief space to address such challenges. Moreover, we present techniques to cope with changes in the environment (e.g., changes in the obstacle map), as well as unforeseen large deviations in the robot's location (e.g., the kidnapped robot problem). We then utilize these techniques to implement the first online replanning scheme in belief space on a physical mobile robot that is robust to changes in the environment and large disturbances. This method demonstrates that belief space planning is a practical tool for robot motion planning.
Development of an automated SNP analysis method using a paramagnetic beads handling robot (2007)

Biological and medical importance of the single nucleotide polymorphism (SNP) has led to development of a wide variety of methods for SNP typing. Aiming for establishing highly reliable and fully automated SNP typing, we have developed the adapter ligation method in combination with the paramagnetic beads handling technology, Magtration(R). The method utilizes sequence specific ligation between the fluorescently labeled adapter and the sample DNAs at the cohesive end produced by a type IIS restriction enzyme. Evaluation of the method using human genomic DNA showed clear discrimination of the three genotypes without ambiguity using the same reaction condition for any SNPs examined. The operations following PCR amplification were automatically performed by the Magtration(R)-based robot that we have previously developed. Multiplex typing of two SNPs in a single reaction by using four fluorescent dyes was successfully preformed at the almost same sensitivity and reliability as the single typing. These results demonstrate that the automated paramagnetic beads handling technology, Magtration(R), is highly adaptable to the automated SNP analysis and that our method best fits to an automated in-house SNP typing for laboratory and medical uses.
Slope Instability from Ground-Water Seepage (1996)

Previous researchers have treated the magnitude and direction of the seepage vector as independent variables and found that seepage, parallel to the surface, does not result in the minimum stable seepage slope. In the present paper, the magnitude and direction of the seepage vector is shown to be uniquely related at the seepage face, and seepage parallel to the slope results is the minimum stable seepage slope. Slope failures under seepage are found to be progressive, and the stable slope angle depends on the direction of the seepage vector. The effect of seepage direction on static liquefaction is investigated, and the seepage directions that initiate static liquefaction depend on the slope angle and soil-unit weight. Analytical predictions of the minimum stable seepage slope compare favorably with experimental results.
Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity (2010)

A stable bacterial community expressing strong chitinolytic activity was constructed by mixing and cultivating chitinolytic bacteria collected from different natural sources. The DNA fragment pattern, after PCR-denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA genes using total DNA prepared from whole cells, indicated that the community was composed of four dominant bacterial species. All four species were isolated on agar medium, and one strain, SAY3, was deduced to be a novel species belonging to a new genus based on the 16S rRNA nucleotide sequence. The other strains showed high similarity in their 16S rRNA sequences to those of previously identified bacteria (Acinetobacter and Microbacterium). Strain SAY3 degraded and utilized larger particles of chitin faster than the community, indicating that it plays an important role in the chitin degradation conferred by the community.
First tungsten complexes with 2′-pyridyl alcoholate ligands: synthesis, structure, and application as novel epoxidation catalysts (1999)

W. A. Herrmann, J. Fridgen, G. M. Lobmaier and M. Spiegler, New J. Chem., 1999, 23, 5 DOI: 10.1039/A808343F