gebhart/patrepeval huggingface dataset:
Variable region heavy chain glycosylation determines the anticoagulant activity of a factor VIII antibody (2010)

The mechanism of action of antibodies inhibiting partially factor VIII (FVIII) activity (type II inhibitor) is still poorly understood. We produced an unusual type II monoclonal antibody, called LE2E9, derived from a patient with mild haemophilia A. The antibody displayed several unexpected structural and functional properties such as glycosylation in the variable region, binding to the FVIII C1 domain, inhibition of maximum 80-90% FVIII activity when in excess over FVIII, and prevention of FVIII binding to von Willebrand factor (VWF). Those unusual characteristics of the antibody prompted multidisciplinary studies to determine its mechanism of action and the role of the FVIII C1 domain. Enzymatic deglycosylation and site-directed mutagenesis indicated that the oligosaccharides do not determine the affinity of the antibody but enhanced its FVIII neutralizing activity. Modification of glycosylation in the variable region of antibodies therefore contributes to the diversity of FVIII type II inhibition and provides a novel strategy with which to modulate the functional activity of antibodies. Investigation of the FVIII C1 domain function led to identification of mutations located in that domain and impairing FVIII binding to VWF as a common cause of mild/moderate haemophilia A. Finally, the cloning of human monoclonal antibodies inhibiting partially FVIII activity opened the way to evaluate such antibodies as a novel type of anticoagulant drug. This concept was validated in animal models of thrombosis. Those studies are expected to have a significant impact on the optimization of treatments for patients with inhibitor as well as for patients with thrombophilia.
Strong room temperature magnetism in highly resistive strained thin films of BiFe0.5Mn0.5O3 (2011)

We report highly resistive strongly ferromagnetic strained thin (∼30 nm) films of BiFe0.5Mn0.5O3 (BFMO) grown on (001) SrTiO3 substrates using pulsed laser deposition. The films are tetragonal with high epitaxial quality and phase-purity. The magnetic moment and coercivity values at room temperature are 90 emu/cc (0.58μB/B-site ion) at H=3 kOe and 274 Oe, respectively. The magnetic transition temperature is strongly enhanced up to ∼600 K, which is ∼500 K higher than for pure bulk BiMnO3. Strained BFMO is a potential room temperature spin filter material for magnetic tunnel devices.
Psychiatric Symptoms Vary With the Severity of Dementia in Probable Alzheimer's Disease (2003)

This cross-sectional study examines relationships among the constellation of psychiatric syndromes in Alzheimer's disease (AD) as a function of dementia severity in 1155 patients with probable AD. The frequency of major depression decreased in severe stages, while agitation, aggression, and psychosis were more frequent in late stages. Major depression was associated with anhedonia, sleep disorders, depressed mood, low self-esteem, anxiety, and hopelessness in mild/moderate and severe stages. Agitation was associated with aggression and psychosis in mild/moderate stages, and psychosis was associated with aggression in moderate/severe stages. In addition, there was a constellation of psychiatric symptoms (e.g., anxiety, wandering, irritability, inappropriate behavior, uncooperativeness, emotional lability) associated with agitation, aggression, and psychosis, which varied according to the severity of the dementia, suggesting a progressive deterioration of frontal-temporal limbic structures. Education and race were independently associated with psychosis. However, while education was associated with psychosis in mild/moderate stages, race was associated with psychosis in moderate/severe stages.
Conductive Organic Plastic Crystals Based on Pyrazolium Imides (2003)

In the Communication by Abu-Lebdeh et al.1, the author list should appear in the following order: P.-J. Alarco, Y. Abu-Lebdeh, and M. Armand. On page 4499, column 2, line 6, the full sentence should read: “Most interestingly, when we doped this plastic crystalline phase with a lithium trifluoromethanesulfonimide salt to increase and possibly add a Li+ ion contribution to the total conductivity, no new phases appeared.” In the concluding paragraph on page 4500, the value 6.2×10-3 S cm−1 should be 6.2×10−4 S cm−1, as in the discussion.
Report from the NSF workshop on workflow and process automation in information systems (1996)

An interdisciplinary research community needs to address challenging issues raised by applying workflow management technology in information systems. This conclusion results from the NSF workshop on Workflow and Process Automation in Information Systems which was held at the State Botanical Garden of Georgia during May 8-10, 1996. The workshop brought together active researchers and practitioners from several communities, with significant representation from database and distributed systems, software process and software engineering, and computer supported cooperative work. The presentations given at the workshop are available in the form of an electronic proceedings of this workshop at http://lsdis.cs.uga.edu/activities/). This report is the joint work of selected representatives from the workshop and it documents the results of significant group discussions and exchange of ideas.
Mouse-Specific Tandem IgY7-SuperMix Immunoaffinity Separations for Improved LC-MS/MS Coverage of the Plasma Proteome (2009)

We report on a mouse specific SuperMix immunoaffinity separation system for separating low-abundance proteins from high and moderate abundance proteins in mouse plasma. When applied in tandem with a mouse IgY7 column that removes the seven most abundant proteins in plasma, the SuperMix column captures more than 100 additional moderate abundance proteins, thus allowing significant enrichment of low-abundance proteins in the flow-through fraction. A side-by-side comparison of results obtained from 2D-LC-MS/MS analyses of flow-through samples from IgY7 and SuperMix columns revealed a nearly 2-fold improvement in the overall proteome coverage. Detection of low-abundance proteins was also enhanced, as evidenced by a more than 2-fold increase in the coverage of cytokines, growth factors, and other low-abundance proteins. Moreover, the tandem separations are automated, reproducible, and allow effective identification of protein abundance differences from LC-MS/MS analyses. Considering the overall reproducibility and increased sensitivity using the IgY7-SuperMix separation system, we anticipate broad applications of this strategy for biomarker discovery using mouse models.
Logic synthesis of low-power ICs with ultra-wide voltage and frequency scaling (2014)

For low-power digital ICs with ultra-wide voltage and frequency scaling (e.g., from the nominal supply voltage to the sub/near-threshold regime), achieving design closure can be a big challenge, especially when speed limits are pushed at very different voltages. This paper shares a practical logic synthesis recipe that helps to fulfill tight timing constraints. Our method includes: i) synthesizing circuits at a high voltage; ii) over-constraining maximal transition time; iii) pruning standard cell library based on cell delay degradation factor across voltages. This approach shows effectiveness on an industrial 90nm low-power micro-controller.
Toxicity Assay-On-Chip for Engineered Nanomaterials (2012)

Abstract not Available.
Lipid remodeling and an altered membrane-associated proteome may drive the differential effects of EPA and DHA treatment on skeletal muscle glucose uptake and protein accretion (2017)

In striated muscle, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have differential effects on the metabolism of glucose and differential effects on the metabolism of protein. We have shown that, despite similar incorporation, treatment of C<sub>2</sub>C<sub>12</sub> myotubes (CM) with EPA but not DHA improves glucose uptake and protein accretion. We hypothesized that these differential effects of EPA and DHA may be due to divergent shifts in lipidomic profiles leading to altered proteomic profiles. We therefore carried out an assessment of the impact of treating CM with EPA and DHA on lipidomic and proteomic profiles. Fatty acid methyl esters (FAME) analysis revealed that both EPA and DHA led to similar but substantials changes in fatty acid profiles with the exception of arachidonic acid, which was decreased only by DHA, and docosapentanoic acid (DPA), which was increased only by EPA treatment. Global lipidomic analysis showed that EPA and DHA induced large alterations in the cellular lipid profiles and in particular, the phospholipid classes. Subsequent targeted analysis confirmed that the most differentially regulated species were phosphatidylcholines and phosphatidylethanolamines containing long-chain fatty acids with five (EPA treatment) or six (DHA treatment) double bonds. As these are typically membrane-associated lipid species we hypothesized that these treatments differentially altered the membrane-associated proteome. Stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics of the membrane fraction revealed significant divergence in the effects of EPA and DHA on the membrane-associated proteome. We conclude that the EPA-specific increase in polyunsaturated long-chain fatty acids in the phospholipid fraction is associated with an altered membrane-associated proteome and these may be critical events in the metabolic remodeling induced by EPA treatment.
Use of a Pressure Reactive Piston to Control Diesel PCCI Operation - A Modeling Study (2006)

&lt;div class="htmlview paragraph"&gt;The heavy-duty diesel engine industry is required to meet stringent emission standards. There is also the demand for more fuel efficient engines by the customer. In a previous study on an engine with variable intake valve closure timing, the authors found that an early single injection and accompanying premixed charge compression ignition (PCCI) combustion provides advantages in emissions and fuel economy; however, unacceptably high peak pressures and rates of pressure-rise impose a severe operating constraint. The use of a Pressure Reactive Piston assembly (PRP) as a means to limit peak pressures is explored in the present work. The concept is applied to a heavy-duty diesel engine and genetic algorithms (GA) are used in conjunction with the multi-dimensional engine simulation code KIVA-3V to provide an optimized set of operating variables. Different sets of PRP parameters (viz. preload, spring constant, damping coefficient and crown mass) were used in an optimization study and the effects of intake valve closure timing, start-of-injection timing, injection duration and exhaust gas recirculation were investigated. The results show that in cases when the combustion is premixed, the inclusion of PRP offers a significant reduction in rate-of-pressure-rise, peak pressure, brake specific fuel consumption and soot and NOx emissions. These benefits are in addition to the advantages offered by variable IVC timing. It is also seen that using PRP could be an effective means to control the timing of the main combustion event and hence the emissions.&lt;/div&gt;
Nuclear Spin Relaxation in Cs Metal (1966)

The characteristic nuclear-magnetic-resonance parameters, Knight shift, line shape and width, and relaxation times have been measured in cesium metal from the melting point to 1.6\ifmmode^\circ\else\textdegree\fi{}K. The nuclear spin relaxation time ${T}_{1}$ is inversely proportional to the temperature from 300 to 7\ifmmode^\circ\else\textdegree\fi{}K, with ${T}_{1}T=0.135\ifmmode\pm\else\textpm\fi{}0.01$ sec \ifmmode^\circ\else\textdegree\fi{}K. For temperatures below 7\ifmmode^\circ\else\textdegree\fi{}K, the relaxation shows another contribution of unknown origin. The Knight shift increases linearly with temperature down to 4\ifmmode^\circ\else\textdegree\fi{}K, following the line previously measured by Gutowsky and McGarvey at higher temperatures. Diffusion narrowing of the magnetic-resonance line is observed at 190\ifmmode^\circ\else\textdegree\fi{}K, but the strong electron-nucleus interactions preclude good diffusion-constant measurements.
Magnetic-Feedback-Based Predistortion Method for Low-Noise Amplifier Linearization (2006)

A predistortion method for CMOS low-noise amplifiers (LNAs) to be used in broadband wireless applications is presented. The method is based on the nulling of the third-order intermodulation distortion of the main amplifier by a highly nonlinear predistortion branch. Maximum nonlinearity product cancellation is ensured by a transformer feedback method. The technique improves linearity in a wide range of input power without significant gain and noise figure (NF) degradation. Simulation results on a 1-V LNA indicate a 10.3-dB improvement in the third-order input intercept point with a degradation of only 1 and 0.44 dB in amplifier gain and NF, respectively. The design is based on a 0.13-mum CMOS technology
Zur Stereochemie der Mutterkornalkaloide vom Agroclavin‐ und Elymoclavin‐Typus. 46. Mitteilung über Mutterkornalkaloide (1958)

Abstract Durch die auf verschiedenen, sterisch eindeutigen Wegen durchgeführte chemische Verknüpfung des Mutterkornalkaloids Agroclavin mit Elymoclavin und D ‐Dihydro‐lysergsäure(I) wurde bewiesen, dass die Ringe C und D im Agroclavin gleiche trans ‐Verknüpfung wie in der D ‐Dihydro‐lysergsäure(I) aufweisen.
Automatic floating-point to fixed-point conversion for DSP code generation (2002)

The development of methodologies for the automatic implementation of floating-point algorithms in fixed-point architectures is required for the minimization of cost, power consumption and time to market of digital signal processing applications. In this paper, a new methodology of implementation in Digital Signal Processors (DSP) under accuracy constraint is presented. In comparison with the existing methodologies, the DSP architecture is completely taken into account for optimizing the execution time under accuracy constraint. The justification and the different stages of our methodology are presented.
Supplementary Figures S1-S2 from The Mitogen-Activated Protein/Extracellular Signal-Regulated Kinase Kinase Inhibitor AZD6244 (ARRY-142886) Induces Growth Arrest in Melanoma Cells and Tumor Regression When Combined with Docetaxel (2023)

Supplementary Figures S1-S2 from The Mitogen-Activated Protein/Extracellular Signal-Regulated Kinase Kinase Inhibitor AZD6244 (ARRY-142886) Induces Growth Arrest in Melanoma Cells and Tumor Regression When Combined with Docetaxel
Autocrine signaling is a key regulatory element during osteoclastogenesis (2014)

Osteoclasts are responsible for bone destruction in degenerative, inflammatory and metastatic bone disorders. Although osteoclastogenesis has been well-characterized in mouse models, many questions remain regarding the regulation of osteoclast formation in human diseases. We examined the regulation of human precursors induced to differentiate and fuse into multinucleated osteoclasts by receptor activator of nuclear factor kappa-B ligand (RANKL). High-content single cell microscopy enabled the time-resolved quantification of both the population of monocytic precursors and the emerging osteoclasts. We observed that prior to induction of osteoclast fusion, RANKL stimulated precursor proliferation, acting in part through an autocrine mediator. Cytokines secreted during osteoclastogenesis were resolved using multiplexed quantification combined with a Partial Least Squares Regression model to identify the relative importance of specific cytokines for the osteoclastogenesis outcome. Interleukin 8 (IL-8) was identified as one of RANKL-induced cytokines and validated for its role in osteoclast formation using inhibitors of the IL-8 cognate receptors CXCR1 and CXCR2 or an IL-8 blocking antibody. These insights demonstrate that autocrine signaling induced by RANKL represents a key regulatory component of human osteoclastogenesis.
Model integrated computing in robot control to synthesize real-time embedded code (2002)

Manufacturing robots present a class of embedded systems with hard real-time constraints. On the one hand controller software has to satisfy tight timing constraints and rigorous memory requirements. Especially nonlinear dynamics and kinematics models are vital to modern model-based controllers and trajectory planning algorithms. Often this is still realized by manually coding and optimizing the software, a labor intensive and error-prone repetitive process. On the other hand shorter design-cycles and a growing number of customer-specific robots demand more flexibility not just in modeling. This paper presents a model-integrated computing approach to automated code synthesis of dynamics models that satisfies the harsh demands by including domain and problem specific constraints prescribed by the robotics application. It is shown that the use of such tailored formalisms leads to very efficient embedded software, competitive with the hand optimized alternative. At the same time it combines flexibility in model specification and usage with the potential for dynamic adaptation and reconfiguration of the model.
Diastereoselective Michael–Claisen Cyclizations of γ-Oxa-α,β-unsaturated Ketones en Route to 5-Oxatetracyclines (2016)

5-Oxatetracyclines were synthesized from d-arabinose using sequential Michael-Claisen cyclization reactions via a 5-oxa-AB enone substrate. The 5-oxatetracyclines were found to have poor stability in aqueous buffer (pH 7.4, 37 °C) and showed little to no inhibition of bacterial growth (S. aureus, E. coli).
Impact of fly ash pretreatment on aerobic treatment of thermomechanical pulping spent liquor (2017)

This study investigated the impact of biomass-based fly ash (FA) pretreatment on the biodegradability of a thermomechanical pulping spent liquor (TMPL) in an aerobic system. In this study, FA was mixed with TMPL under the conditions of 6 wt.% based on TMPL, 25°C and 10 h, which removed a part of recalcitrant organic materials and resulted in 68.0, 40.0, 60.1, 81.2 and 48.3% reductions in chemical oxygen demand (COD), biochemical oxygen demand (BOD), total organic carbon (TOC), lignin and sugar, respectively. FA-pretreated TMPL pressate (FA-TMPL) was biologically treated in an aerobic system of sequencing batch reactor (SBR). The performance of the biological treatment with and without FA pretreatment was studied in two parallel SBRs over three months. The combination of FA and biological treatments removed 97.3% of COD, 98.3% of BOD, 96.3% of lignin, 99.5% of sugar, and 98.1% of TOC. Without FA pretreatment, the biological system removed 87.3% of COD, 89% of BOD, 81.6% of lignin, 98.6% of sugars, and 90.5% of TOC. The results also confirmed that the settling ability of sludge, which was indicated as a sludge volume index, was reduced from 109.3 mL/g to 53.5 mL/g. In addition, the advantages of using FA pretreatment in aerobic systems were discussed in detail. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:370-378, 2018.
Haplotype association and mutation analysis of the transglutaminase 1 gene for prenatal exclusion of lamellar ichthyosis (2000)

Lamellar ichthyosis (LI) is an autosomal recessive keratinization disorder of the skin. Genetic heterogeneity has been shown for the disease and there is evidence for the involvement of the transglutaminase 1 (TGM1) gene on chromosome 14q11. We have previously identified chromosome 14q11 haplotypes associated with ichthyosis in the Norwegian population. In this paper we describe antenatal exclusion of ichthyosis in two Norwegian families by chromosome 14q11 haplotype association and direct mutation analysis. In one pregnancy, the 11-week old fetus at risk for LI was found to share only one disease-associated haplotype. A subsequent mutation analysis of the TGM1 gene in fetal DNA revealed that the fetus carried a novel 3795A-->T transversion. The affected proband was compound heterozygous for the mutations 3795A-->T and 3239G-->C resulting in an Asp430Val and a Val379Leu, respectively. In another LI family, the 11-week old fetus was found to be heterozygous for the 14q11 haplotype associated with the disease. Subsequent mutation analysis revealed that the fetus was heterozygous for the 2526A-->G transition in the splice site of intron 5 whereas the proband was homozygous for the same mutation. Our results show that haplotyping can be a useful tool for prenatal diagnosis in diseases with genetic heterogeneity.
Design, simulation and testing of a hybrid liquid desiccant for independent control of temperature and humidity (2015)

In this paper the design, simulation and testing of a hybrid liquid desiccant system for a case study in Taiwan is presented, in the scope of the European project nanoCOOL. The designed system has been constructed and is being tested at laboratory scale. After the set-up of the system, it will be sent to Taipei for demonstration in real scale conditions, in the Taiwan Building Technology Center. The demonstration site comprises two locker rooms in a swimming pool of the university, with high internal humidity generation, low sensible heat ratio, and high external humidity levels due to sub-tropical humid climate present in Taiwan.
Structures of human thymidylate synthase R163K with dUMP, FdUMP and glutathione show asymmetric ligand binding (2010)

Thymidylate synthase (TS) is a well validated target in cancer chemotherapy. Here, a new crystal form of the R163K variant of human TS (hTS) with five subunits per asymmetric part of the unit cell, all with loop 181-197 in the active conformation, is reported. This form allows binding studies by soaking crystals in artificial mother liquors containing ligands that bind in the active site. Using this approach, crystal structures of hTS complexes with FdUMP and dUMP were obtained, indicating that this form should facilitate high-throughput analysis of hTS complexes with drug candidates. Crystal soaking experiments using oxidized glutathione revealed that hTS binds this ligand. Interestingly, the two types of binding observed are both asymmetric. In one subunit of the physiological dimer covalent modification of the catalytic nucleophile Cys195 takes place, while in another dimer a noncovalent adduct with reduced glutathione is formed in one of the active sites.
Comparison of Electron Beam Computed Tomography and Technetium Stress Testing in Differentiating Cause of Dilated Versus Ischemic Cardiomyopathy (2005)

Given the high sensitivity of nuclear testing and EBT, these tests may prove to be an effective screen before angiography in patients with congestive heart failure of unclear cause.
Rotor Temperature Estimation for Saliency-based Control of Induction Motors using Symmetrical Transient Reluctance (2021)

Performance of flux-oriented control of induction motors is highly impacted by accurate knowledge of rotor temperature. Rotor temperature mismatches lead to deviations of flux and torque calculated by motor models, also modifying the actual magnetic saturation level. Saturation level, at the same time, strongly influences the parameters of machine saliencies used for speed sensorless control. This paper proposes a novel rotor temperature estimation method that is integrated in a sensorless control scheme, using a voltage step excitation method and extracting rotor slotting information as control signal. The same excitation method is also used to calculate the symmetrical value of the transient reluctance using a new parallel signal processing scheme. The amplitude of the symmetrical transient reluctance presents a temperature-dependent behaviour, which is exploited by a controller leading to accurate rotor temperature estimation. Proposed temperature estimation requires almost no additional resources. Experimental results are presented to verify excellent sensorless FOC performance combined with accurate rotor temperature estimation.