gebhart/patrepeval huggingface dataset:
Targeted overexpression of mitochondrial catalase protects against cancer chemotherapy-induced skeletal muscle dysfunction (2016)

The loss of strength in combination with constant fatigue is a burden on cancer patients undergoing chemotherapy. Doxorubicin, a standard chemotherapy drug used in the clinic, causes skeletal muscle dysfunction and increases mitochondrial H2O2 We hypothesized that the combined effect of cancer and chemotherapy in an immunocompetent breast cancer mouse model (E0771) would compromise skeletal muscle mitochondrial respiratory function, leading to an increase in H2O2-emitting potential and impaired muscle function. Here, we demonstrate that cancer chemotherapy decreases mitochondrial respiratory capacity supported with complex I (pyruvate/glutamate/malate) and complex II (succinate) substrates. Mitochondrial H2O2-emitting potential was altered in skeletal muscle, and global protein oxidation was elevated with cancer chemotherapy. Muscle contractile function was impaired following exposure to cancer chemotherapy. Genetically engineering the overexpression of catalase in mitochondria of muscle attenuated mitochondrial H2O2 emission and protein oxidation, preserving mitochondrial and whole muscle function despite cancer chemotherapy. These findings suggest mitochondrial oxidants as a mediator of cancer chemotherapy-induced skeletal muscle dysfunction.
Tumor-Directed Radiation and the Immunotoxin SS1P in the Treatment of Mesothelin-Expressing Tumor Xenografts (2006)

Combination of SS1P with tumor-directed radiation results in enhanced antitumor activity against mesothelin-expressing tumor xenografts. This effect was seen when either low or high doses of radiation were used.
Utilisation of Flexible Nano-Structured PEDOT Electropolymerised From Ionic Liquids for One Step (4-electron) Oxygen Reduction Reaction (2013)

Abstract not Available.
Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, A gambogic acid derivative, in human leukemia HL-60 cells (2007)

Gambogic acid (GA) is the major active ingredient of gamboge secreted from a Chinese traditional medicine Garcinia hanburryi possessing potent anti-tumor activity. N-(2-ethoxyethyl)gambogamide (NG-18), a derivative of GA, also efficiently inhibits proliferation of cultured human tumor cells. The inhibition effect of NG-18 is associated with its ability to induce apoptosis. In the present study, NG-18 markedly induced leukemia HL-60 cells apoptosis, and the extrinsic and intrinsic apoptosis pathways were activated almost at the same time. NG-18-induced tumor cell apoptosis was associated with up-regulation of pro-apoptotic Bcl-2 family member Bax, and downregulation of anti-apoptotic protein Bcl-2. The NG-18-induced apoptosis was blocked completely by a pan-caspase inhibitor Z-VAD-FMK, indicating that caspases were functionally and actively involved in this process. The specific inhibition of caspase-8 activity using Z-IETD-FMK significantly blocked NG-18-induced apoptosis. In contrast, inhibition of other initiator caspases, caspase-2 or -9, using Z-VDVAD-FMK or Z-LEHD-FMK respectively had no effect on NG-18-induced apoptosis. Altogether, our data demonstrated that NG-18-induced apoptosis was dependent on caspases and caspase-8 acted as a key executor in the event.
Valsartan Upregulates Kir2.1 in Rats Suffering from Myocardial Infarction via Casein Kinase 2 (2015)

AT1 receptor antagonist valsartan reduces CK2 activation, increases Kir2.1 expression and thereby ameliorates IK1 remodeling after MI in the rat model.
Isolation and Characterisation of a Recombinant, Precursor form of Lysosomal Acid α‐Glucosidase (1995)

Glycogenosis type II (GSD II, Pompe disease) is an autosomal recessive lysosomal storage disease that results from a deficiency of acid alpha-glucosidase (GAA). Patients with this disorder are unable to break down lysosomal glycogen, which consequently accumulates in the lysosome. To evaluate enzyme replacement therapy for GSD II patients, we have expressed human GAA cDNA in Chinese hamster ovary-K1 cells utilising a vector that places the cDNA under the transcriptional control of the human polypeptide chain elongation factor 1 alpha gene promoter. A clonal cell line that secreted precursor recombinant GAA at approximately 18 mg.l-1.day-1 was identified. The precursor recombinant GAA was purified to homogeneity, had a molecular mass of 110 kDa as measured by SDS/PAGE, and was shown to have pH optima and kinetic parameters similar to those of GAA purified from human tissues. The partial N-terminal amino acid sequence of recombinant GAA conformed to that derived from the nucleotide sequence of the cloned cDNA. The recombinant enzyme was taken up by cultured fibroblasts and skeletal muscle cells from GSD II patients, and was shown to correct the storage phenotype. Endocytosed GAA was localised to the lysosome and showed evidence of intracellular processing to a more mature form. Activity levels increased up to twice the normal value and uptake was prevented if cells were cultured in the presence of mannose 6-phosphate.
Collagen-hydroxyapatite composite substitute and bone marrow nuclear cells on posterolateral spine fusion in sheep (2019)

The effects of a large collagen-hydroxyapatite (COL-HA) substitute with or without isolated bone marrow nuclear cells (BMNC) on spine fusion were evaluated using a two-level posterolateral spinal fusion model in sheep. Six sheep each were included in both the COL-HA and autograft groups. In each animal, spine fusions were performed at two levels by randomization with or without BMNC. After an observation period of 4.5 months, the fusion blocks (length = 50 mm) were harvested and evaluated by micro-CT with respect to fusion rate and microarchitecture, and histology was performed to qualitatively assess new bone morphology. BMNC supplementation did not lead to significant differences in fusion rates or microarchitectural properties in the both groups ( p = 0.56). Porosity decreased significantly in the COL-HA group ( p = 0.002), suggesting a relatively fast degradation of COL-HA. Qualitative histology assessment revealed quick reabsorption of COL-HA replaced by newly formed bone, and this new bone morphology was similar to autograft. Interestingly, COL-HA generated significant amounts of new bone in vivo which were similar to autograft, and the presence of BMNC seemed to enhance this process. Apart from porosity, the comparable fusion rate and similar microarchitectural parameters suggested equal performance of COL-HA and autograft with respect to spine fusion and bone quality. In conclusion, the COL-HA with BMNC demonstrated comparable fusion rates as the autograft. COL-HA was capable of forming new bone and was revealed to be similar to the autograft with respect to microarchitectural properties apart from porosity of the fusion blocks.
Crystal Structure of Phosphate Binding Protein Labeled with a Coumarin Fluorophore, a Probe for Inorganic Phosphate, (1998)

Crystal structures are presented for the A197C mutant of Escherichia coli phosphate binding protein (PBP) and the same mutant labeled at Cys197 with N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic phosphate. The resulting high-fluorescence state of the coumarin and the ability of this coumarin to monitor the conformational changes associated with inorganic phosphate binding are interpreted in terms of the specific interactions of MDCC with the protein. The structure helps to explain why this particular label gives a high-fluorescence state on binding inorganic phosphate, while several other related labels do not, and hence aids our general understanding of environmentally sensitive fluorescence probes on proteins.
Dihedral angle of biphenyl in solution and the molecular force field (1973)

The dihedral angle between the rings of biphenyl is determined to be 32 ± 2° in the molten and solution states. Observed vibrational frequency shifts on going from the planar configuration held in the crystal to the non-planar solution state are compared with computed frequency shifts. To improve the accuracy in the computed frequencies the force constants were refined. The values of the force constants associated with the inter-ring bond are discussed.
The ion channel TRPM7 is required for B cell lymphopoiesis (2018)

The transient receptor potential (TRP) family is a large family of widely expressed ion channels that regulate the intracellular concentration of ions and metals and respond to various chemical and physical stimuli. TRP subfamily M member 7 (TRPM7) is unusual in that it contains both an ion channel and a kinase domain. TRPM7 is a divalent cation channel with preference for Ca<sup>2+</sup> and Mg<sup>2+</sup> It is required for the survival of DT40 cells, a B cell line; however, deletion of TRPM7 in T cells does not impair their development. We found that expression of TRPM7 was required for B cell development in mice. Mice that lacked TRPM7 in B cells failed to generate peripheral B cells because of a developmental block at the pro-B cell stage. The loss of TRPM7 kinase activity alone did not affect the proportion of peripheral mature B cells or the development of B cells in the bone marrow. However, supplementation with a high concentration of extracellular Mg<sup>2+</sup> partially rescued the development of TRPM7-deficient B cells in vitro. Thus, our findings identify a critical role for TRPM7 ion channel activity in B cell development.
Polyurethane heart valves: Fatigue failure, calcification, and polyurethane structure (1997)

Six flexible-leaflet prosthetic heart valves, fabricated from a polyetherurethaneurea (PEUE), underwent long-term fatigue and calcification testing. Three valves exceeded 800 million cycles without failure. Three valves failed at 775, 460, and 544 million cycles, respectively. Calcification was observed with and without associated failure in regions of high strain. Comparison with similar valves fabricated from a polyetherurethane (PEU) suggests that the PEU is likely to fail sooner as a valve leaflet. Localized calcification developed in PEUE leaflets at the primary failure site of PEU leaflets, close to the coaptation region of the three leaflets. The failure mode in PEU valves had the appearance of abrasion wear associated with calcification. High strains in the same area may render the PEUE leaflets vulnerable to calcification. Intrinsic calcification of this type, however, is a long-term phenomenon unlikely to cause early valve failure. Both polymers performed similarly during static in vitro and in vivo calcification testing and demonstrated a much lesser degree of calcification than bioprosthetic types of valve materials. Polyurethane valves can achieve the durabilities required of an implantable prosthetic valve, equaling the fatigue life of currently available bioprosthetic valves. © 1997 John Wiley & Sons, Inc.
Primary Inhibition of Hypocotyl Growth and Phototropism Depend Differently on Phototropin-Mediated Increases in Cytoplasmic Calcium Induced by Blue Light (2003)

Abstract The phototropin photoreceptors transduce blue-light signals into several physiological and developmental responses in plants. A transient rise in cytoplasmic calcium (Ca2+) that begins within seconds of phototropin 1 (phot1) excitation is believed to be an important element in the transduction pathways leading to one or more of the phot1-dependent responses. The goal of the present work was to determine whether the Ca2+ response was necessary for (a) the inhibition of hypocotyl elongation that develops within minutes of the irradiation, and (b) hypocotyl phototropism (curved growth of the stem in response to asymmetric illumination). After determining that pulses of light delivering photon fluences of between 1 and 1,000 μmol m-2 induced growth inhibition mediated by phot1 without significant interference from other photosensory pathways, the effect of blocking the Ca2+ rise was assessed. Treatment of seedlings with a Ca2+ chelator prevented the rise in cytoplasmic Ca2+ and prevented phot1-mediated growth inhibition. However, the same chelator treatment did not impair phot1-mediated phototropism. Thus, it appears that the early, transient rise in cytoplasmic Ca2+ is an important intermediary process in at least one but not all phot1-signaling pathways.
Preparation of Chiral Fluorine Compounds from (2S,3S)-3-Phenylglycidol (1989)

Abstract Some potentially useful chiral fluorine compounds have been synthesized starting from (2S,3S)-3-phenylglycidol.
THE USE OF MARBLE CUTTING RESIDUES AS A SUBSTITUTE FLUXING AGENT FOR IRON ORE PELLET PRODUCTION (2014)

The State of Espirito Santo has one of the largest reserves of marble in Brazil and an industrial park with about 1,000 companies, wish have more than half of looms installed in the country. The biggest problem faced by these manufacturing industries is the generation of waste rock, in the form of abrasive slurry from the cutting and polishing of marble. These wastes produced during the step of cutting the marble are dumped in open tanks causing environmental pollution. A quantity of 35000 tons of solid waste with a grain size of less than 150 mm is generated per month. Such residues have high contents of limestone flux, and have potential for use as input in the production of iron ore pellets which are used as a ferrous cargo in the ironmaking and steelmaking processes. For the conventional production of iron ores pellets, limestone flux, whose composition is of CaCO3 and MgCO3 is used as an important source of MgO and CaO for the primary industrial ferrous plants. In these study pellets containing marble residue were produced in a pilot plant. The samples were analysed in industrial materials laboratories and subsequently subjected to tests of physical properties producing data of the chemical, physical and metallurgical properties according to the Norm ISO (International Organization for Standardization). At the end of this work, it was found that the residue is suitable for the pelletizing process. We conclude that the crude/green and the fired pellets made from marble waste have characteristics very similar to those of the commercial pellets.
NOTE ON PALUDESTRINA JENKINSI (1907)

Journal Article NOTE ON PALUDESTRINA JENKINSI Get access E. A SMITH E. A SMITH Search for other works by this author on: Oxford Academic Google Scholar Journal of Molluscan Studies, Volume 7, Issue 4, March 1907, Pages 203–204, https://doi.org/10.1093/oxfordjournals.mollus.a066168 Published: 01 March 1907 Article history Received: 09 November 1906 Published: 01 March 1907
Tubulin, Gq, and Phosphatidylinositol 4,5-Bisphosphate Interact to Regulate Phospholipase Cβ1 Signaling (1997)

The cytoskeletal protein, tubulin, has been shown to regulate adenylyl cyclase activity through its interaction with the specific G protein alpha subunits, Galphas or Galphai1. Tubulin activates these G proteins by transferring GTP and stabilizing the active nucleotide-bound Galpha conformation. To study the possibility of tubulin involvement in Galphaq-mediated phospholipase Cbeta1 (PLCbeta1) signaling, the m1 muscarinic receptor, Galphaq, and PLCbeta1 were expressed in Sf9 cells. A unique ability of tubulin to regulate PLCbeta1 was observed. Low concentrations of tubulin, with guanine nucleotide bound, activated PLCbeta1, whereas higher concentrations inhibited the enzyme. Interaction of tubulin with both Galphaq and PLCbeta1, accompanied by guanine nucleotide transfer from tubulin to Galphaq, is suggested as a mechanism for the enzyme activation. The PLCbeta1 substrate, phosphatidylinositol 4,5-bisphosphate, bound to tubulin and prevented microtubule assembly. This observation suggested a mechanism for the inhibition of PLCbeta1 by tubulin, since high tubulin concentrations might prevent the access of PLCbeta1 to its substrate. Activation of m1 muscarinic receptors by carbachol relaxed this inhibition, probably by increasing the affinity of Galphaq for tubulin. Involvement of tubulin in the articulation between PLCbeta1 signaling and microtubule assembly might prove important for the intracellular governing of a broad range of cellular events.
The IMGT/HLA sequence database. (2000)

The IMGT/HLA database (wwwebi.ac.uk/imgt/hla/) specialises in sequences of the polymorphic genes of the HLA system, the humanmajor histocompatibility complex (MHC). This complex is located within the 6p213 region on the short arm of human chromosome 6 and contains more than 220 genes of diverse function. Many of the genes encode proteins of the immune system and these include the 21 highly polymorphic HLA genes, which influence the outcome of clinical transplantation and confer susceptibility to a wide range of non-infectious diseases. The database contains sequences for all HLA alleles officially recognised by the WHO Nomenclature Committee for Factors of the HLA System and provides users with online tools and facilities for their retrieval and analysis. These include allele reports, alignment tools, and detailed descriptions of the source cells. The online submission tool allows both new and confirmatory sequences to be submitted directly to the WHO Nomenclature Committee. The latest version (release 1.10.0 April 2001) contains 1329 HLA alleles, 61 HLA related sequences, derived from around 3350 component sequences from the EMBL/ GenBank/DDBJ databases. The IMGT/HLA database provides a model that will be extended to provide specialist databases for polymorphic MHC genes of other species.
Towards the creation of quasi-phasematched devices using femtosecond laser micromachining (2006)

The feasibility of using femtosecond laser micro-machining to create Gallium Arsenide (GaAs) quasi-phasematched devices from single GaAs wafers is investigated. We describe machining of the structure and details of the machining quality achievable in GaAs.
Use of organic polymers for improvement of coking potential of poor coking coal (2019)

As the reserve of prime coking coal is low, there is a good amount of research going on worldwide to improve the coking potential of non-coking coal. Researchers have revealed the importance of hydrogen generation/transfer to improve the coking potential of coal. Based on this hypothesis, in this paper, the authors have tried to improve the coking potential of non-coking coal by adding different polymers. The polymers are derived from formaldehyde and phenol derivatives. Coking potential has been tested through conventional tests like crucible swelling number. Thermogravimetric mass spectroscopy (TG-MS) has been used to get an idea of plasticity and hydrogen generation in the plastic zone. High-temperature microscopy has also been used to evaluate the swelling of coal. Results indicate that the addition of polymers in a small percentage has an effect on hydrogen generation in the plastic zone, and thus, it can improve the coking potential of non-coking coal.
Vision System With Ranging For Maneuvering In Space (1986)

Automated rendezvous, stationkeeping, and docking, as well as robot vison for space construction, require a compact, rugged system to provide two-and three-dimensional (range) images. The system proposed in this paper consists of a module having a transmitter array of laser diodes and a single detector/receiver that covers the full field of view (FOV) of the module. Each diode in the array is imaged on a pixel in the FOV. Sinusoidal intensity modulation is used such that the range at each pixel can be determined by phase comparison of the transmitted and received signals. The system FOV can be expanded by using multiple modules. The laser diode modulation frequency is initially low to determine coarse range without ambiguities. It is then increased in sequential steps to reach the desired accuracy for a given range. Because the scanning is accomplished by individually illuminating elements in the diode array, the scanning is achieved with no moving parts, and the pixels in the FOV can be randomly accessed. Preliminary results demonstrated a 15 cm range accuracy with an uncooperative target at a range of 13 m in bright sunlight.
Rings with quasi-continuous right ideals (1999)

Rings in which each right ideal is quasi-continuous (right π-rings) are shown to be a direct sum of semisimple artinian square full ring and a right square free ring. Among other results it is also shown that (i) a nonlocal right continuous indecomposable right π-ring is either simple artinian or a ring of matrices of a certain type, and (ii) an indecomposable non-local right continuous ring is both a right and a left π-ring if and only if it is a right q-ring. In particular, a non local indecomposable right q-ring is a left q-ring.
Identification of a Transcriptomic Network Underlying the Wrinkly and Smooth Phenotypes of Vibrio fischeri (2020)

<i>Vibrio fischeri</i> is a cosmopolitan marine bacterium that oftentimes displays different colony morphologies, switching from a smooth to a wrinkly phenotype in order to adapt to changes in the environment. This wrinkly phenotype has also been associated with increased biofilm formation, an essential characteristic for <i>V. fischeri</i> to adhere to substrates, to suspended debris, and within the light organs of sepiolid squids. Elevated levels of biofilm formation are correlated with increased microbial survival of exposure to environmental stressors and the ability to expand niche breadth. Since <i>V. fischeri</i> has a biphasic life history strategy between its free-living and symbiotic states, we were interested in whether the wrinkly morphotype demonstrated differences in its expression profile in comparison to the naturally occurring and more common smooth variant. We show that genes involved in major biochemical cascades, including those involved in protein sorting, oxidative stress, and membrane transport, play a role in the wrinkly phenotype. Interestingly, only a few unique genes are specifically involved in macromolecule biosynthesis in the wrinkly phenotype, which underlies the importance of other pathways utilized for adaptation under the conditions in which <i>Vibrio</i> bacteria are producing this change in phenotype. These results provide the first comprehensive analysis of the complex form of genetic activation that underlies the diversity in morphologies of <i>V. fischeri</i> when switching between two different colony morphotypes, each representing a unique biofilm ecotype.<b>IMPORTANCE</b> The wrinkly bacterial colony phenotype has been associated with increased squid host colonization in <i>V. fischeri</i> The significance of our research is in identifying the genetic mechanisms that are responsible for heightened biofilm formation in <i>V. fischeri</i> This report also advances our understanding of gene regulation in <i>V. fischeri</i> and brings to the forefront a number of previously overlooked genetic networks. Several loci that were identified in this study were not previously known to be associated with biofilm formation in <i>V. fischeri</i>.
Analysis of a Depth Model - Common Offset Depth Migration as a Velocity Analysis Tool (1990)

Time domain processing has been extensively developed during the eighties to the stage that, with DMO, good quality images can be formed for most situations. However, in structurally complex areas, converting the time image to a correct depth image has been a serious problem. Recently, prestack depth migration has attracted more and more attention because of its capability of placing the image at the correct location, laterally and vertically.