gebhart/patrepeval huggingface dataset:
Copper Binding and Redox Activity of α-Synuclein in Membrane-Like Environment (2023)

α-Synuclein (αSyn) constitutes the main protein component of Lewy bodies, which are the pathologic hallmark in Parkinson's disease. αSyn is unstructured in solution but the interaction of αSyn with lipid membrane modulates its conformation by inducing an α-helical structure of the <i>N</i>-terminal region. In addition, the interaction with metal ions can trigger αSyn conformation upon binding and/or through the metal-promoted generation of reactive oxygen species which lead to a cascade of structural alterations. For these reasons, the ternary interaction between αSyn, copper, and membranes needs to be elucidated in detail. Here, we investigated the structural properties of copper-αSyn binding through NMR, EPR, and XAS analyses, with particular emphasis on copper(I) coordination since the reduced state is particularly relevant for oxygen activation chemistry. The analysis was performed in different membrane model systems, such as micellar sodium dodecyl sulfate (SDS) and unilamellar vesicles, comparing the binding of full-length αSyn and <i>N</i>-terminal peptide fragments. The presence of membrane-like environments induced the formation of a copper:αSyn = 1:2 complex where Cu<sup>+</sup> was bound to the Met1 and Met5 residues of two helical peptide chains. In this coordination, Cu<sup>+</sup> is stabilized and is unreactive in the presence of O<sub>2</sub> in catechol substrate oxidation.
Chelyabinsk Airburst, Damage Assessment, Meteorite Recovery, and Characterization (2013)

The asteroid impact near the Russian city of Chelyabinsk on 15 February 2013 was the largest airburst on Earth since the 1908 Tunguska event, causing a natural disaster in an area with a population exceeding one million. Because it occurred in an era with modern consumer electronics, field sensors, and laboratory techniques, unprecedented measurements were made of the impact event and the meteoroid that caused it. Here, we document the account of what happened, as understood now, using comprehensive data obtained from astronomy, planetary science, geophysics, meteorology, meteoritics, and cosmochemistry and from social science surveys. A good understanding of the Chelyabinsk incident provides an opportunity to calibrate the event, with implications for the study of near-Earth objects and developing hazard mitigation strategies for planetary protection.
Dynamics of molecular mechanisms underlying ovarian oxytocin secretion. (1995)

In the ruminant ovary, synthesis and secretion of oxytocin begin in the granulosa cells of the preovulatory follicle and are markedly stimulated by the surge of LH and FSH. Luteinization of the granulosa cells results in a further increase in oxytocin gene expression, but translation of mRNA appears to be retarded because the peak concentration of luteal oxytocin occurs later than the maximal accumulation of the message. Several hormones have been shown to stimulate oxytocin secretion from granulosa and luteal cells in vivo or in vitro. However, the role of prostaglandin F2 alpha (PGF2 alpha) in regulating luteal oxytocin secretion has perhaps received more study than other hormones. The mechanism of action of PGF2 alpha has been shown to encompass a phosphoinositide cascade and activation of protein kinase C, events that are associated with luteal secretion of oxytocin. Protein kinase C phosphorylation of the actin-binding protein myristolated alanine-rich C kinase substrate (MARCKS) may be required for exocytosis of oxytocin.
Regeneration of a Spinal Ligament after Total Lumbar Disk Arthroplasty in Primates (2009)

Total disk arthroplasty (TDA) is a new procedure that replaces the intervertebral disk space with an artificial motion segment and necessitates the resection of the anterior longitudinal ligament (ALL). We assessed whether a collagen-based graft made from porcine small-intestine submucosa (SIS) can be used as a regenerative scaffold to restore the function and structure of the ALL in the lumbar spine. A total of 10 mature male baboons underwent TDA at L5-L6 using one of two treatments: (1) TDA only (n = 5) or (2) TDA combined with SIS (n = 5). Six months postoperatively, mock revision surgery was performed to assess tissue adhesions followed by non-destructive multidirectional flexibility testing of the spinal segment. The vertebral segments were then processed for histology. The tissue adhesion score was 2.8 +/- 0.8 in the TDA only group and 1.8 +/- 1.4 in the TDA-SIS group (p = 0.2). Segmental range of motion and the length of the neutral zone were similar in both groups. Histology showed that the SIS scaffold led to an organized ligamentous structure with a significantly (p = 0.027) higher thickness (2.18 +/- 0.25 mm) compared to the connective tissue structure in the TDA-only group (1.66 +/- 0.33 mm). We concluded that using a SIS bioscaffold after TDA did not lead to increased great vessel adhesion while its use facilitated the formation of highly organized ligamentous tissues. However, the SIS- induced and newly formed ligamentous tissue anterior to the spinal segment did not lead to a measurable limitation of spinal extension.
The Effect of Explosive Detonation Characteristics on Shaped Charge Performance (1974)

Abstract : The jet formation caused by the collapse of a shaped charge liner is dependent upon the pressure delivered to the liner wall by the detonating explosive. As a shaped charge liner is collapsed axisymmetrically to the charge axis by a detonation wave to form a jet and a slug, the liner material experiences plastic deformation at exceedingly high strain rates. If the jet is presumed to form simultaneously on the axis, and then stretch uniformly, a one dimensional approximation shows that strain rates characteristic of this process are 10,000/second. It is assumed that the jet is a ductile material which remains continuous during stretching. The purpose of this study was to determine the influence of explosive filler on shaped charge warhead performance.
Stepwise multiphoton activation fluorescence reveals a new method of melanin detection (2013)

The stepwise multiphoton activated fluorescence (SMPAF) of melanin, activated by a continuous-wave mode near infrared (NIR) laser, reveals a broad spectrum extending from the visible spectra to the NIR and has potential application for a low-cost, reliable method of detecting melanin. SMPAF images of melanin in mouse hair and skin are compared with conventional multiphoton fluorescence microscopy and confocal reflectance microscopy (CRM). By combining CRM with SMPAF, we can locate melanin reliably. However, we have the added benefit of eliminating background interference from other components inside mouse hair and skin. The melanin SMPAF signal from the mouse hair is a mixture of a two-photon process and a third-order process. The melanin SMPAF emission spectrum is activated by a 1505.9-nm laser light, and the resulting spectrum has a peak at 960 nm. The discovery of the emission peak may lead to a more energy-efficient method of background-free melanin detection with less photo-bleaching.
Metabolization of the Artificial Secondary Metabolite 4-Hydroxybenzoate in ubiC-Transformed Tobacco (1997)

Transgenic Nicotiana tabacum cell cultures formed a new, artificial secondary metabolite, i.e. 4-hydroxybenzoate (4HB), by expression of the bacterial gene ubiC, which encodes chorismate pyruvate-lyase. 4HB was converted in the cells to two different glucosides, i.e. 4-O-(l-fi-D-gIucosyl)benzoic acid (4HBOG) and 4HB l-/7-D-glucosyl ester (4HBCOOG). The same metabolization was also observed with exogenously fed 4HB. This glucosylation is catalyzed by constitutively expressed glucosyltransferases which could be detected in cell-free extracts of transgenic and untransformed cells in the same activity. The enzymes forming the two respective glucosides differ in their pH optima, their affinity for 4HB and their regulation. Both in vivo and in cell-free extracts, 4HBOG is the main product at low 4HB concentration, and 4HBCOOG at high 4HB concentration. Whereas 4HBOG is accumulated as an apparently stable secondary metabolite, 4HBCOOG presents a metabolically active form of 4HB. After feeding of [U-"C]4HB, a transient accumulation of 4HBCOOG was followed by incorporation of radioactive 4HB into the cell wall, suggesting that 4HBCOOG, but not 4HBOG serves as precursor of cell wall bound 4HB.
WebCQ -detecting and delivering information changes on the web (2000)

WebCQ is a prototype system for large-scale Web information monitoring and delivery. It makes heavy use of the structure present i n h ypertext and the concept of continual queries. In this paper we discuss both mechanisms that We-bCQ uses to discover and detect changes to the World Wide Web (the Web) pages e ciently, and the methods to notify users of interesting changes with a personalized customization. The WebCQ system consists of four main components: a c hange detection robot that discovers and detects changes, a proxy cache service that reduces communication tra cs to the original information servers, a personalized presentation tool that highlights changes detected by W ebCQ sentinels, and a change noti cation service that delivers fresh information to the right users at the right time. A salient feature of our change detection robot is its ability to support various types of web page sentinels for detecting, presenting, and delivering interesting changes to web pages. This paper describes the WebCQ system with an emphasis on general issues in designing and engineering a large-scale information change monitoring system on the Web.
A palladium–dithiooxamide complex (1994)

Single crystals of bis[dithiooxamido(1-)-S,S']palladium 0.5-methanol solvate 0.5-hydrate, [Pd(C 2 H 3 N 2 S 2 ) 2 ].0.5CH 3 OH.0.5H 2 O, are obtained from a methanolic solution of K 2 PdCl 6 and dithiooxamide. This complex crystallizes in the monoclinic system (space group I2/a). The structure can be described as an array of neutral centrosymmetric Pd(LH 3 ) 2 molecules (where LH 3 =C 2 H 3 N 2 S 2 ). Each Pd atom has a near perfect square-planar environment of S atoms. The unsubstituted dithiooxamide molecules are planar and show a cis conformation which is observed for the first time in a crystal structure. The methanol molecule is disordered
Involvement of the Checkpoint Protein Mec1p in Silencing of Gene Expression at Telomeres in Saccharomyces cerevisiae (2000)

Yeast strains with a mutation in the MEC1 gene are deficient in the cellular checkpoint response to DNA-damaging agents and have short telomeres (K. B. Ritchie, J. C. Mallory, and T. D. Petes, Mol. Cell. Biol. 19:6065-6075, 1999; T. A. Weinert, G. L. Kiser, and L. H. Hartwell, Genes Dev. 8:652-665, 1994). In wild-type yeast cells, genes inserted near the telomeres are transcriptionally silenced (D. E. Gottschling, O. M. Aparichio, B. L. Billington, and V. A. Zakian, Cell 63:751-762, 1990). We show that mec1 strains have reduced ability to silence gene expression near the telomere. This deficiency was alleviated by the sml1 mutation. Overexpression of Mec1p also resulted in a silencing defect, although this overexpression did not affect the checkpoint function of Mec1p. Telomeric silencing was not affected by mutations in several other genes in the Mec1p checkpoint pathway (null mutations in RAD9 and CHK1 or in several hypomorphic rad53 alleles) but was reduced by a null mutation of DUN1. In addition, the loss of telomeric silencing in mec1 strains was not a consequence of the slightly shortened telomeres observed in these strains.
Capture of circulatory endothelial progenitor cells and accelerated re-endothelialization of a bio-engineered stent in human ex vivo shunt and rabbit denudation model (2011)

In this proof-of-concept study, we have demonstrated that the bio-engineered EPC-capture stent, Genous™ R™ stent, is effective in EPC capture, resulting in accelerated re-endothelialization and reduced thrombogenicity.
Crystallographic properties and mechanical behaviour of titanium hydride layers grown on titanium implants (2004)

International audience
Social Media Alert and Response to Threats to Citizens (2012)

Social media, such as blogs, Twitter, and information portals, have emerged as the dominant communication mechanism of society. Exploiting such input to gain awareness of an incident is a critical direction for research in effective emergency management. In this paper we present an overview of the S
Hepatitis C Virus Viremia in SCID -> BNX Mouse Chimera (1995)

Chimpanzees are currently the only nonhuman animal model for reproducible propagation of hepatitis C virus (HCV). A chimeric mouse model was used for the induction of hepatitis C viremia, using BNX (beige/nude/X-linked immunodeficient) mice preconditioned by total body irradiation and reconstituted with SCID mouse bone marrow cells. HCV-infected liver fragments from patients with HCV RNA-positive sera were transplanted under the kidney capsule of the chimeric mice. HCV-specific RNA sequences were detected by reverse transcriptase nested polymerase chain reaction (RT-PCR) in serum of approximately 50% of grafted animals. In addition, normal liver specimens were incubated with HCV serum and transplanted into chimeric mice, leading to viremia in approximately 25% of animals. Sequential histologic evaluation of the liver implants, from day 2 to week 14 after transplantation, revealed loss of lobular architecture within the implants. However, viremia persisted for 10-50 days after transplantation. These results offer a new HCV model.
<title>Comparison of fluorescence properties of wild type and the W15F mutant of horse liver alcohol dehydrogenase</title> (1994)

Horse liver alcohol dehydrogenase is a homodimeric protein; each subunit has two tryptophan residues that are in distinctly different microenvironments. Trp-15 is located on the surface and Trp-314 is buried at the intersubunit interface. Steady-state and time-resolved fluorescence and phosphorescence studies have enabled the assignment of parameters, e.g., quantum yield, emission maximum, decay times, to the individual tryptophan residues of the protein. We have prepared, by site-directed mutagenesis, the mutated W15F protein and have characterized its fluorescence properties. We show that the Trp-314 of the mutant experiences an apolar microenvironment, but that the fluorescence decay and exposure to solute quenchers of the mutant are somewhat different than was expected from the assignments for the wild type.
Homology modeling and monoclonal antibody binding of the Der f 7 dust mite allergen (2010)

The group 7 allergens are important allergenic specificities for mite-sensitive patients and may need to be incorporated into new diagnostic and therapeutic strategies. However, little is known about their biological and structural features. Position-specific iterative BLAST showed that they had strong ancestral homology to two related families of lipid-binding proteins, namely, the bactericidal permeability-increasing (BPI) proteins and the odorant-binding protein. A three-dimensional model of Der f 7 made with the Phyre and SWISS-MODEL homology-modeling servers showed a close match with the human BPI coordinates used for its construction. The binding of the monoclonal antibody HD12 known to block IgE binding could be blocked by the linear sequence (46GILDF50) with a critical role for L48 or F50. These hydrophobic residues were located on a surface loop of the model. The properties of Der f 7 that can be deduced from the model provide avenues for further characterizing these allergens, their IgE binding structures and biological properties that can enhance allergenicity.
Multiple jammer localization and characterization based on time-frequency analysis (2018)

Jammers pose a threat to the reliability of satellite navigation systems. The vast majority of these devices generate broadband interference by rapidly sweeping wide bandwidths with continuous waves. Their waveforms fall into the set of nonstationary signals that can be conveniently analysed through timefrequency transforms. Based on a novel use of the S-transform, the present paper describes a framework for estimating the modulation characteristics of multiple jammers and therewith the respective locations. The performance are evaluated by means of numerical simulations for two jammers. When the jamming waveforms are well characterized, the locations of their sources can be estimated by trilateration with accuracies below ten meters within a square area of side of one kilometre.
A fast two-channel projection algorithm for stereophonic acoustic echo cancellation (2002)

We propose a new fast projection algorithm for stereophonic acoustic echo cancellation. This algorithm can be viewed as a generalization of the extended two-channel LMS algorithm which takes into account the correlation between the input signals. Moreover, this algorithm fits naturally with the framework of affine projection techniques extended to the two-channel case. Its computational complexity is less than half the complexity of the fastest two-channel RLS versions. Simulation results show the obtained performance.
Pulmonary Arterial Hypertension: A Rare Complication of Primary Sjögren Syndrome (2007)

Primary Sjögren syndrome (pSS) is a fairly common autoimmune disease with glandular and extraglandular manifestations. Pulmonary involvement mainly corresponds to small airways and interstitial lung disease. Pulmonary arterial hypertension (PAH) is rare: to our knowledge, only 32 cases have been reported in pSS patients to date. PAH is a disease of the small pulmonary arteries characterized by vascular proliferation and remodeling, resulting in a progressive increase in pulmonary vascular resistance, and, ultimately, right ventricular failure and death. We report 9 new cases of pSS-associated PAH with a complete assessment including clinical characteristics (of both PAH and pSS), hemodynamic parameters, medical management, and outcome. We also review the 19 fully documented PAH patients with pSS reported in the English-language literature, therefore analyzing a total of 28 cases (27 women; mean age at PAH diagnosis, 50 +/- 11 yr; range, 23-68 yr). Functional impairment at diagnosis was severe, with a New York Heart Association (NYHA) functional class of III or IV in most cases. Seven of 15 (47%) patients for whom data were available had history or evidence of right heart failure at PAH diagnosis. Hemodynamic parameters were moderate to severe with a mean pulmonary artery pressure of 44 +/- 11 mm Hg (range, 24-60 mm Hg) and a cardiac index of 2.91 +/- 0.72 Lmin(-1)m(-2) (range, 1.36-3.88 Lmin(-1)m(-2)). Standard PAH therapy (endothelin receptor antagonists, phosphodiesterase type 5 inhibitors, or prostanoids) was initially effective in some patients but had short-term and long-term failures. Some patients were treated with first-line immunosuppressants alone leading to improvement in some, but second-line standard PAH therapy was added in all cases thereafter. The best treatment strategy remains to be defined. Estimated survival rates were low (73% and 66% at 1 and 3 years, respectively). Compared with pSS patients without PAH, patients with pSS-associated PAH had Raynaud phenomenon, cutaneous vasculitis, and interstitial lung disease significantly more frequently. They also more frequently had antinuclear, anti-Ro/SSA, and anti-RNP autoantibodies, as well as positive rheumatoid factor and hypergammaglobulinemia. These data suggest that systemic vasculopathy, B-cell activation, and autoimmunity could play a role in the pathophysiology of pSS-associated PAH. In conclusion, this report underlines the rarity and severity of PAH in pSS patients. The best therapeutic regimen remains to be defined but should include standard PAH therapy and/or immunosuppressants.
Mutational and phenotypic spectrum of OTOF-related auditory neuropathy in Koreans: eliciting reciprocal interaction between bench and clinics (2018)

This study included the largest Korean DFNB9 cohort to date and proposed a set of the most frequent four OTOF variants, allowing the potential prioritization of exons during Sanger sequencing. Further, a significant association of p.Arg1939Gln homozygotes with poor residual hearing was observed. We may have to suspect p.Arg1939Gln homozygosity in cases of poor auditory thresholds in ANSD children with putative negative OTOF variants solely screened by NGS. Reciprocal feedback between bench and clinics regarding DFNB9 would complement each other.
Experimental Study on the Fabrication of Fiber Reinforced Aluminium by Squeeze Casting (1982)

Squeeze casting increases the productivity of the fabrication of composite materials, whereas the theoretically expected strength is not easily attainable by it due to many factors. The present investigation has been carried out systematically to find the optimum conditions for squeeze casting applied to fabrication of SiC fiber reinforced aluminium. From the experiment, it is found that the selected conditions satisfy the requirements, i.e., soundness and high strength. The results obtained are summarized as follows : l) Squeeze pressure must exceed 500 kg/cm2 to produce composites free from cavities. 2) Infiltration speed does not affect the microstructures and tensile strength. 3) There exists a suitable temperature range to fabricate reliably sound composite materials with a given volume fraction of fiber. The range can be presented by considering a parameter depending on both temperatures of fiber and molten aluminium immediately before infiltration.
Lower oxidation states of selenium. 2. Potentiometric study involving tetravalent selenium, tetraselenium(2+), octaselenium(2+), and three other low oxidation states of selenium in a chloroaluminate melt (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLower oxidation states of selenium. 2. Potentiometric study involving tetravalent selenium, tetraselenium(2+), octaselenium(2+), and three other low oxidation states of selenium in a chloroaluminate meltRasmus. Fehrmann and Niels J. BjerrumCite this: Inorg. Chem. 1977, 16, 8, 2089–2094Publication Date (Print):August 1, 1977Publication History Published online1 May 2002Published inissue 1 August 1977https://doi.org/10.1021/ic50174a054RIGHTS & PERMISSIONSArticle Views42Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (733 KB) Get e-Alerts Get e-Alerts
A 1V 2.3/spl mu/W Biomedical Signal Acquisition IC (2006)

A 1V 2.3uW biomedical signal acquisition IC is fabricated in a 0.35μ CMOS process. It consists of an LIMA and an 11b ADC. The OTA achieves an optimum trade-off between noise and power consumption with rail-to-rail output. A pseudo-S/H circuit is incorporated in the OTA. The modified successive-approximation ADC allows rail-to-rail inputs