gebhart/patrepeval huggingface dataset:
Expression in E. coli and purification of a chimeric p22‐NS3 recombinant antigen of Hepatitis C Virus (HCV) (1993)

A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a starting point for the design of an HCV single all-encompassing antigen for a blood screening assay.
InGaAsP-InP nanoscale waveguide-coupled microring lasers with submilliampere threshold current using Cl/sub 2/--N/sub 2/-based high-density plasma etching (2005)

We demonstrate InGaAsP-InP nanoscale waveguide-coupled microring lasers fabricated with inductively coupled plasma (ICP) using Cl/sub 2/--N/sub 2/-based gas mixtures. To fabricate optical waveguides, the requirement of anisotropic profiles and sidewall smoothness is stringent. In particular, for strongly lateral confined nanoscale waveguides used for microring resonators, the degree of anisotropy and sidewall smoothness is critical in determining the device performance. For optoelectronic devices such as microcylinder and microring lasers, the requirement of low active-layer damage further adds to the stringency. We show that the excellent etching property of high-density plasma using Cl/sub 2/--N/sub 2/-based gas mixtures satisfies the requirements. The good etching results are demonstrated by using the etching method to realize room-temperature operations of the InGaAsP-InP waveguide-coupled microring lasers. For the waveguide-coupled microring lasers with a diameter of 10 /spl mu/m, we obtained submilliampere threshold current.
40  A reliable method for simplifying flexible uretero‐renoscopy in patients with urinary diversions (2006)

Introduction: Access to the upper urinary tract to exclude or treat recurrent transitional cell carcinoma (TCC) is not infrequently required in patients who have previously undergone urinary diversion in the management of invasive bladder carcinoma. This can be a technically difficult procedure. We describe a method which simplifies the diagnosis and treatment of upper tract TCC posturinary diversion. Patients and Methods: Four patients who had previously required urinary diversion (3 ileal conduit, 1 ileal neobladder) underwent flexible uretero‐renoscopy after antegrade skinny‐needle renal puncture and placement of a guidewire down the ureter into the ileal conduit. The guidewire is then externalised. A second guidewire is placed in a retrograde fashion using a double lumen catheter or an exchange guidewire set, and this second guidewire is then used as a guide for insertion of the flexible ureteroscope. This guidewire may be replaced repeatedly during the procedure to facilitate multiple biopsies. Results: All patients were treated successfully and without complication from placement of the percutaneous guidewire. Operating time was under one hour in all cases. Conclusions: Flexible uretero‐renoscopy of the upper urinary tract postileal urinary diversion can be made easier and less time consuming by preliminary placement of a percutaneous antegrade guidewire.
Determining Driver Phone Use by Exploiting Smartphone Integrated Sensors (2015)

This paper utilizes smartphone sensing of vehicle dynamics to determine driver phone use, which can facilitate many traffic safety applications. Our system uses embedded sensors in smartphones, i.e., accelerometers and gyroscopes, to capture differences in centripetal acceleration due to vehicle dynamics. These differences combined with angular speed can determine whether the phone is on the left or right side of the vehicle. Our low infrastructure approach is flexible with different turn sizes and driving speeds. Extensive experiments conducted with two vehicles in two different cities demonstrate that our system is robust to real driving environments. Despite noisy sensor readings from smartphones, our approach can achieve a classification accuracy of over 90 percent with a false positive rate of a few percent. We also find that by combining sensing results in a few turns, we can achieve better accuracy (e.g., 95 percent) with a lower false positive rate. In addition, we seek to exploit the electromagnetic field measurement inside a vehicle to complement vehicle dynamics for driver phone sensing under the scenarios when little vehicle dynamics is present, for example, driving straight on highways or standing at roadsides.
Organometallic electrochemistry (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic electrochemistryRaymond E. Dessy and Leo A. BaresCite this: Acc. Chem. Res. 1972, 5, 12, 415–421Publication Date (Print):December 1, 1972Publication History Published online1 May 2002Published inissue 1 December 1972https://pubs.acs.org/doi/10.1021/ar50060a004https://doi.org/10.1021/ar50060a004research-articleACS PublicationsRequest reuse permissionsArticle Views278Altmetric-Citations66LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Extended morphological processing: a practical method for automatic spot detection of biological markers from microscopic images (2010)

Our method achieved effective spot extraction under various image conditions, including aggregated target spots, poor signal-to-noise ratio, and large variations in the background intensity. Furthermore, it has no restrictions with respect to the shape of the extracted spots. The features of our method allow its broad application in biological and biomedical image information analysis.
Interparticle collision of natural sediment grains in water (2001)

Elastohydrodynamic theory and measurements of particle impacts on an inclined glass plane in water are used to investigate the mechanics of interparticle collisions in sediment‐transporting flows. A collision Stokes number is proposed as a measure of the momentum of an interparticle collision versus the viscous pressure force in the interstitial gap between colliding particles. The viscous pressure force opposes motion of the particles on approach and rebound. A Stokes number of between 39 and 105 is estimated as the critical range below which particle impacts are completely viscously damped and above which impacts are partially elastic. The critical Stokes number is shown to roughly coincide with the Bagnold number transition between macroviscous and grain inertial debris flows and the transition between damped and partially elastic bed load transport saltation impacts. The nonspherical nature of natural particles significantly alters the motion of the center of mass after a partially elastic collision. The normal to the point of contact between the particles does not necessarily go through the center of mass. Thus normal rebound of the center of mass may not occur. A model of particle motion after rebound for particles of arbitrary shape, conserving both linear and angular momentum, is proposed.
The Behavior of α-Ketoglutaric Acid and Its Ester in Alkaline Media. I-II II. a-Ketoglutaric Acid (1960)

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Initial Service Provider DevOps concept, capabilities and proposed tools (2015)

This report presents a first sketch of the Service Provider DevOps concept including four major management processes to support the roles of both service and VNF developers as well as the operator in a more agile manner. The sketch is based on lessons learned from a study of management and operational practices in the industry and recent related work with respect to management of SDN and cloud. Finally, the report identifies requirements for realizing SP-DevOps within an combined cloud and transport network environment as outlined by the UNIFY NFV architecture.
Hydrophobic Residues of the D2 Dopamine Receptor Are Important for Binding and Signal Transduction (1995)

Dopamine receptors belong to the seven transmembrane helix-containing, G protein-coupled receptor superfamily. Mutagenesis studies suggest that dopamine and its analogues interact with aspartate-114 in helix 3 and two helix 5 serines (194 and 197) of the D2 receptor. In addition to these amino acids, hydrophobic residues within the receptor core may be important not only for binding but also for receptor activation. Described is a site-directed mutagenesis investigation into the roles of these hydrophobic residues in the long isoform of the human D2 receptor. Replacement of helix 6 phenylalanines (389 or 390) with alanines resulted in disrupted binding to several agonists and antagonists and impaired inhibition of adenylyl cyclase activity. Replacement of the helix 5 phenylalanine-198 with an alanine selectively disrupted [3H]N-0437 binding, whereas the affinities for other agonists and antagonists remained unchanged. This mutant remained functionally intact when stimulated with dopamine or bromocriptine. Replacement of the helix 7 phenylalanine-411 or the helix 6 leucine-387 with alanines produced receptors that bound agonists well but were unable to inhibit adenylyl cyclase. Based on these data, two conserved helix 6 phenylalanines (389 and 390) appear to be crucial for ligand binding, and phenylalanine-411 in helix 7 and leucine-387 in helix 6 may be important for propagating conformational changes from the agonist binding site(s) to G protein coupling domain(s) of the D2 receptor.
Cost effectiveness of geophysical inversions in mineral exploration: Applications at San Nicolas (2001)

Effective mineral exploration programs maximize the benefit of appropriate technologies in order to increase cost effectiveness by optimizing the use of drilling, reducing risks, and increasing the speed of discovery. In other words, correct use of available tools can allow exploration programs to find more ore, faster, with less expense.
Reaction of pyrrolidine with P-1-aziridinyl-N,N,N',N'-tetramethylphosphonic diamides (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReaction of pyrrolidine with P-1-aziridinyl-N,N,N',N'-tetramethylphosphonic diamidesPhilip E. SonnetCite this: J. Org. Chem. 1967, 32, 1, 248–250Publication Date (Print):January 1, 1967Publication History Published online1 May 2002Published inissue 1 January 1967https://pubs.acs.org/doi/10.1021/jo01277a071https://doi.org/10.1021/jo01277a071research-articleACS PublicationsRequest reuse permissionsArticle Views30Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
An update on the pharmacological strategies in the treatment of HIV-1-associated adipose redistribution syndromes (2014)

The most commonly used strategies for HALS reversion have included modification of host-dependent factors, including those related to HIV-1 infection and those associated with ART. Preventive and medical strategies have been associated with moderate success. The only intervention that offers an immediate aesthetical improvement for patients with HALS so far has been plastic surgery.
Nanotube‐like processes facilitate material transfer between photoreceptors (2021)

Neuronal communication is typically mediated via synapses and gap junctions. New forms of intercellular communication, including nanotubes (NTs) and extracellular vesicles (EVs), have been described for non-neuronal cells, but their role in neuronal communication is not known. Recently, transfer of cytoplasmic material between donor and host neurons ("material transfer") was shown to occur after photoreceptor transplantation. The cellular mechanism(s) underlying this surprising finding are unknown. Here, using transplantation, primary neuronal cultures and the generation of chimeric retinae, we show for the first time that mammalian photoreceptor neurons can form open-end NT-like processes. These processes permit the transfer of cytoplasmic and membrane-bound molecules in culture and after transplantation and can mediate gain-of-function in the acceptor cells. Rarely, organelles were also observed to transfer. Strikingly, use of chimeric retinae revealed that material transfer can occur between photoreceptors in the intact adult retina. Conversely, while photoreceptors are capable of releasing EVs, at least in culture, these are taken up by glia and not by retinal neurons. Our findings provide the first evidence of functional NT-like processes forming between sensory neurons in culture and in vivo.
Macrophages inhibit human osteosarcoma cell growth after activation with the bacterial cell wall derivative liposomal muramyl tripeptide in combination with interferon-γ (2014)

This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-γ may be of relevance for the optimization of L-MTP-PE therapy in osteosarcoma patients.
Sorghum genetic, genomic, and breeding resources (2021)

Sorghum research has entered an exciting and fruitful era due to the genetic, genomic, and breeding resources that are now available to researchers and plant breeders. As the world faces the challenges of a rising population and a changing global climate, new agricultural solutions will need to be developed to address the food and fiber needs of the future. To that end, sorghum will be an invaluable crop species as it is a stress-resistant C<sub>4</sub> plant that is well adapted for semi-arid and arid regions. Sorghum has already remained as a staple food crop in many parts of Africa and Asia and is critically important for animal feed and niche culinary applications in other regions, such as the United States. In addition, sorghum has begun to be developed into a promising feedstock for forage and bioenergy production. Due to this increasing demand for sorghum and its potential to address these needs, the continuous development of powerful community resources is required. These resources include vast collections of sorghum germplasm, high-quality reference genome sequences, sorghum association panels for genome-wide association studies of traits involved in food and bioenergy production, mutant populations for rapid discovery of causative genes for phenotypes relevant to sorghum improvement, gene expression atlas, and online databases that integrate all resources and provide the sorghum community with tools that can be used in breeding and genomic studies. Used in tandem, these valuable resources will ensure that the rate, quality, and collaborative potential of ongoing sorghum improvement efforts is able to rival that of other major crops.
Calprotectin as a smoldering activity detection tool and renal prognosis biomarker in ANCA associated vasculitis (2018)

Worsening of renal function, hematuria, rising proteinuria and non-decreasing ANCA correlated with higher levels of serum calprotectin at recruitment. Low urine calprotectin was found in patients with sclerotic histology. Calprotectin during remission in ANCA vasculitis may be useful to identify subclinical inflammation and worse renal prognosis patients.
Design and Synthesis of Novel HIV-1 Protease Inhibitors Comprising a Tertiary Alcohol in the Transition-State Mimic (2006)

HIV-1 protease inhibitors are important in the most frequently used regimen for the treatment of HIV/AIDS, the highly active antiretroviral therapy (HAART). For patients with access to this treatment, an HIV infection is no longer lethal, but rather a manageable, chronic infection. However, the HIV-1 protease inhibitors are generally associated with serious shortcomings such as adverse events, development of drug resistance and poor pharmacokinetic properties. Most of the approved inhibitors suffer from high protein binding, rapid metabolism and/or low membrane permeability. In this project, novel HIV-1 protease inhibitors comprising a rarely used tertiary alcohol in the transition-state mimic were designed, synthesized and evaluated. The rationale behind the design was to achieve ‘masking’ of the tertiary alcohol by for example, intramolecular hydrogen bonding, which was believed could enhance transcellular transport. A reliable synthetic protocol was developed and a series of highly potent inhibitors was obtained exhibiting excellent membrane permeation properties in a Caco-2 cell assay. However, the cellular antiviral potencies of these compounds were low. In an attempt to improve the anti-HIV activity, microwave-accelerated, palladium-catalyzed cross-coupling reactions and aminocarbonylation of aryl bromide precursors were employed to produce P1'-extended test compounds. Inhibitors demonstrating up to six times higher antiviral effect were obtained, the best derivatives having para 3- or 4-pyridyl elongations in P1'. Fast metabolic degradation was observed in liver microsome homogenate, which is believed, at least partly, to be attributable to benzylic oxidation of the indanol P2 group of the inhibitors. To enable facile variation of the P2 side chain a new synthetic route was developed using an enantiomerically pure, benzyl-substituted epoxy carboxylic acid as the key intermediate. Cyclic and amino-acid-residue-derived P2 groups were evaluated, and inhibitors equipotent to the series containing an indanol moiety were produced.
Cascaded InGaSb quantum dot mid-infrared LEDs (2022)

We demonstrate cascaded, mid-infrared light-emitting diodes with quantum dot based active regions. Cascading is achieved through highly reverse-biased AlInAsSb tunnel junctions that serve to connect the successive InGaSb quantum dot active regions. Temperature-dependent characterization of the output irradiance as a function of the current and voltage indicates that the cascade architecture has minimal leakage currents in contrast to earlier single-stage devices and provides carrier recycling with a concomitant increase in irradiance. The results show that cascaded architectures can be applied to quantum dot platforms and that the quantum efficiency of the active region limits the overall device efficiency.
Dimebon alters hippocampal amyloid pathology in 3xTg-AD mice. (2012)

A double blind, placebo-controlled phase II study revealed that the antihistamine, Dimebon® (dimebolin, latrepirdine) improved cognition in Alzheimer disease (AD) patients compared to placebo controls. However, the Phase III CONNECTION trial failed to demonstrate significant differences between dimebon and placebo treatments. Despite the controversial therapeutic outcomes in the treatment of AD, dimebon's mechanism(s) of action within the brain remain unclear. In the present study, we evaluated the effects of dimebon upon β-amyloid (Aβ), tau and astrocytes in the hippocampus of triple transgenic (3xTg-AD) mice, which develop AD-like pathology in an age-dependent manner. At age 6.5 months, prior to the development of Aβ plaques in the hippocampus, male and female 3xTg-AD mice, received a daily intraperitoneal injection of 0.1 % dimebon or saline for 1.5 months. At 8 months, quantitative immunohistochemistry revealed a significant reduction in hippocampal/subicular APP/Aβ in dimebon-treated mice, whereas protein bioassay found no change in full length APP, soluble Aβ(1-40) and Aβ(1-42), Aβ oligomers, BACE1 and GFAP levels between groups. Interestingly, the number of the hippocampal APP/Aβ plaques in female and male dimebon-treated mice was higher compared to gender-matched control mice. Dimebon did not alter hippocampal tau levels. Furthermore, dimebon protects SH-SY5Y neurons against Aβ toxicity and promotes GFAP expression in primary mouse astrocyte cultures. Our findings demonstrate that dimebon in vivo modifies hippocampal APP/Aβ pathology and in vitro protects against Aβ toxicity promoting cell survival and activates astrocytes.
Cost-based Deployment Planning for Components in Smart Item Environments (2006)

Middleware for smart items can facilitate the coordinated execution of distributed components for early data processing. These components can be deployed to different nodes in the middleware or even on the smart item itself. While such flexibility is desirable to fulfil various information requirements from backend applications, it poses the problem of finding good distributions for given components and component compositions. In this paper, we present an approach for deployment planning of components based on their expected resource consumption under a certain load. Using an example scenario we analyse the influencing factors on placement decisions. These are integrated into an evaluation schema. The concept is proven by an implementation, which is used to find suitable distributions for the example scenario with varying loads. We present the results and discuss the effect of different load settings.
Integrated Stress Response Mediates Epithelial Injury in Mechanical Ventilation (2017)

Ventilator-induced lung injury (VILI) is a severe complication of mechanical ventilation that can lead to acute respiratory distress syndrome. VILI is characterized by damage to the epithelial barrier with subsequent pulmonary edema and profound hypoxia. Available lung-protective ventilator strategies offer only a modest benefit in preventing VILI because they cannot impede alveolar overdistension and concomitant epithelial barrier dysfunction in the inflamed lung regions. There are currently no effective biochemical therapies to mitigate injury to the alveolar epithelium. We hypothesize that alveolar stretch activates the integrated stress response (ISR) pathway and that the chemical inhibition of this pathway mitigates alveolar barrier disruption during stretch and mechanical ventilation. Using our established rat primary type I-like alveolar epithelial cell monolayer stretch model and in vivo rat mechanical ventilation that mimics the alveolar overdistension seen in acute respiratory distress syndrome, we studied epithelial responses to mechanical stress. Our studies revealed that the ISR signaling pathway is a key modulator of epithelial permeability. We show that prolonged epithelial stretch and injurious mechanical ventilation activate the ISR, leading to increased alveolar permeability, cell death, and proinflammatory signaling. Chemical inhibition of protein kinase RNA-like endoplasmic reticulum kinase, an upstream regulator of the pathway, resulted in decreased injury signaling and improved barrier function after prolonged cyclic stretch and injurious mechanical ventilation. Our results provide new evidence that therapeutic targeting of the ISR can mitigate VILI.