gebhart/patrepeval huggingface dataset:
Tyrosinase as a dual reporter gene for both photoacoustic and magnetic resonance imaging (2011)

Reporter genes are useful scientific tools for analyzing promoter activity, transfection efficiency, and cell migration. The current study has validated the use of tyrosinase (involved in melanin production) as a dual reporter gene for magnetic resonance and photoacoustic imaging. MCF-7 cells expressing tyrosinase appear brown due to melanin. Magnetic resonance imaging of tyrosinase-expressing MCF-7 cells in 300 μL plastic tubes displayed a 34 to 40% reduction in T1 compared to normal MCF-7 cells when cells were incubated with 250 μM ferric citrate. Photoacoustic imaging of tyrosinase-expressing MCF-7 cells in 700 μm plastic tubes displayed a 20 to 57-fold increase in photoacoustic signal compared to normal MCF-7 cells. The photoacoustic signal from tyrosinase-expressing MCF-7 cells was significantly greater than blood at 650 nm, suggesting that tyrosinase-expressing cells can be differentiated from the vasculature with in vivo photoacoustic imaging. The imaging results suggest that tyrosinase is a useful reporter gene for both magnetic resonance and photoacoustic imaging.
Three Mating Type-Like Loci in Candida glabrata (2003)

Candida glabrata, the second most prevalent Candida species colonizing humans, possesses three mating type-like (MTL) loci (MTL1, MTL2, and MTL3). These loci contain pairs of MTL genes with their respective coding regions on complementary Crick and Watson DNA strands. Each pair of genes is separated by a shared intergenic promoter region, the same configuration found at the mating type loci of Saccharomyces cerevisiae. Two of the MTL loci, MTL1 and MTL2, contain either the MTLa1/MTLa2 configuration or the MTLalpha1/MTLalpha2 configuration in different strains. All but one of the 38 tested C. glabrata strains were either aaalpha or aalphaalpha. One test strain was alphaalphaalpha. Based on the mating type genotype, the MTL genes at the MTL1 or MTL2 loci, and the size of the XbaI fragment harboring MTL1 or MTL2, four classes of C. glabrata strains (I, II, III, and IV) were distinguished. Northern analysis revealed that strains were either a-expressors or alpha-expressors and that expression always reflected the genotype of either the MTL1 or MTL2 locus, depending on the class. The expression pattern in each class, therefore, is similar to that observed in S. cerevisiae, which harbors two silent cassette loci, HMR and HML, and the expression locus MAT. High-frequency phenotypic switching between core phenotypes in an alpha-expressing, but not in an a-expressing, strain modulated the level of MTL expression, suggesting a possible relationship between core phenotypic switching and mating.
Microindentation of the Young Porcine Ocular Lens (2009)

Debate regarding the mechanisms of how the eye changes focus (accommodation) and why this ability is lost with age (presbyopia) has recently been rejoined due to the advent of surgical procedures for the correction of presbyopia. Due to inherent confounding factors in both in vivo and in vitro measurement techniques, mechanical modeling of the behavior of the ocular lens in accommodation has been attempted to settle the debate. However, a paucity of reliable mechanical property measurements has proven problematic in the development of a successful mechanical model of accommodation. Instrumented microindentation was utilized to directly measure the local elastic modulus and dynamic response at various locations in the lens. The young porcine lens exhibits a large modulus gradient with the highest modulus appearing at the center of the nucleus and exponentially decreasing with distance. The loss tangent was significantly higher in the decapsulated lens and the force waveform amplitude decreased significantly upon removal of the lens capsule. The findings indicate that localized measurements of the lens’ mechanical properties are necessary to achieve accurate quantitative parameters suitable for mechanical modeling efforts. The results also indicate that the lens behaves as a crosslinked gel rather than as a collection of individual arched fiber cells.
A perimetric learner's index (1997)

The learner's index highlights those first field tests from eyes with normal visual fields, that deviate significantly from a normal experienced result in the direction of a learner's result. Patients showing significant learner's index are candidates for repeated visual field testing.
Purification and characterization of a novel bacteriocin produced by Lactobacillus crustorum MN047 isolated from koumiss from Xinjiang, China (2016)

The growing emergence of antibiotic-resistant bacteria in the food industry needs to be controlled with effective antimicrobials. In this study, bacteriocin MN047 A (BMA) was found to have antibacterial activity against multidrug-resistant bacteria. It was produced by Lactobacillus crustorum MN047, which was first isolated from koumiss, a traditional fermented dairy product from Xinjiang Autonomous Region, China. It was purified by ammonium sulfate precipitation, ion-exchange chromatography, and reversed-phase chromatography. It had a low molecular mass of 1,770.89 Da according to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the sequence was identified as QLPWQILGIVAGMFQA by liquid chromatography-tandem mass spectrometry analysis and MASCOT searching. It was proteinaceous in nature: the bacteriocin was digested by protease but not by α-amylase or lipase. It showed broad pH toleration (pH 2-11), good thermostability, and good storage stability. It had a broad inhibitory spectrum, including both gram-positive and gram-negative bacteria. Growth curve and time-kill kinetics indicated that it was bactericidal to the indicator strains, and this finding was verified by scanning electron microscope and transmission electron microscope after treatment with BMA. As well, BMA halted the growth of Staphylococcus aureus and Escherichia coli in the G1 and G2/M phases according to cell-cycle analysis by flow cytometry, indicating that BMA had comprehensive inhibitory effects against foodborne pathogens.
Simple Analysis on History Pedagogics in Middle School (2006)

When learning history,students are always tired of studying and afraid of difficulty.In order to overcome this problem and enhance learning effects,this article puts forward the sum total of effective ways from the angle of“learning methods”,such as,taking for one's own needs,questioning ,integration,striving for change and distillation.
Sentiment Pen (2019)

In this paper, we discuss the assessment of the emotional state of the user from digitized handwriting for implicit human-computer interaction. The proposed concept exemplifies how a digital system could recognize the emotional context of the interaction. We discuss our approach to emotion recognition and the underlying neurophysiological mechanisms. To verify the viability of our approach, we have conducted a series of tests where participants were asked to perform simple writing tasks after being exposed to a series of emotionally-stimulating video clips from EMDB[6], one set of four clips per each quadrant on the circumplex model of emotion[28]. The user-independent Support Vector Classifier (SVC) built using the recorded data shows up to 66% accuracy for certain types of writing tasks for 1 in 4 classification (1. High Valence, High Arousal; 2. High Valence, Low Arousal; 3. Low Valence, High Arousal; 4. Low Valence, Low Arousal). In the same conditions, a user-dependent classifier reaches an average of 70% accuracy across all 12 study participants. While future work is required to improve the classification rate, this work should be seen as proof-of-concept for emotion assessment of users while handwriting aiming to motivate research on implicit interaction while writing to enable emotion-sensitivity in mobile and ubiquitous computing.
Generalized Gauged Thirring Model on Curved Space-Times (1993)

We analyse the interacting theory of charged fermions, scalars, pseuso-scalars and photons propagating in 2-dimensional curved spacetime in detail. For certain values of the coupling constants the theory reduces to the gauged Thirring model and for others the Schwinger model incurved spacetime. It is shown that the interaction of the fermions with the pseudo-scalars shields the electromagnetic interaction, and that the non-minimal coupling of the scalars to the gravitational field amplifies the Hawking radiation. We solve the finite temperature and density model by using functional techniques and in particular derive the exact equation of state. The explicit temperature and curvature dependence of the chiral condensate is found. When the electromagnetic field is switched off the model reduces to a conformal field theory. We determine the physically relevant expectation values and conformal weights of the fundamental fields in the theory.
Neonatal inflammation and primary afferent terminal plasticity in the rat dorsal horn (2003)

Abnormal or excessive activity related to pain and injury in early life may alter normal synaptic development and lead to changes in somatosensory processing. The aim of the current study was to define the critical factors that determine long-term plasticity in spinal cord afferent terminals following neonatal inflammation. Hindpaw inflammation was produced in neonatal rat pups with 5 or 25 microl 2% carrageenan, and 5 or 25 microl complete Freund's adjuvant (CFA). All groups displayed a clear inflammatory response that recovered in 2 weeks in all but the 25 microl CFA group, who had persistent chronic inflammation confirmed by histological examination of the paw at 8 weeks. The 25 microl CFA group was also the only group that displayed a significant expansion of the sciatic and saphenous nerve terminal field in lamina II of the dorsal horn at 8 weeks, using wheat-germ agglutinin-horse radish peroxidase transganglionic labelling. This effect was not accompanied by changes in dorsal root ganglion (DRG) cell number, expression of activating transcription factor 3 (ATF3), or alterations in calcitonin gene related peptide (CGRP) or isolectin B4 binding; and was not mimicked by partial nerve damage. No long-term change in mechanical or thermal behavioural sensory thresholds was seen in any group. Lower dose CFA caused an acute, reversible expansion of terminal fields in lamina II in neonatal animals, while CFA did not produce this effect in adults. The duration and effect of neonatal inflammation is therefore dependent on the type and volume of inflammatory agent used. The expansion of afferent terminals in lamina II following neonatal CFA inflammation is maintained into adulthood if the inflammation is also maintained, as seen following 25 microl CFA. This effect is not seen in adult animals, emphasising the plasticity of the nervous system early in development.
Identification of the mokH Gene Encoding Transcription Factor for the Upregulation of Monacolin K Biosynthesis in Monascus pilosus (2009)

Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus. The monacolin K biosynthetic gene cluster, mokA-mokI, has been characterized in Monascus pilosus. The mokH gene encoding Zn(II)2Cys6 binuclear DNA binding protein is assumed to be an activator for monacolin K production. In this study, the mokH gene was cloned and driven by the glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter for overexpression in M. pilosus. The transformants containing an extra copy of the mokH gene were obtained and verified by PCR and Southern hybridization. The transcripts of mokH in the transformants were expressed significantly higher than those of the wild-type strain. The transformants were stably inherited through the next generation, as determined by observation of the enhanced green fluorescent protein (EGFP). The transformant T-mokH1 also showed a 1.7-fold higher production of monacolin K than the wild-type strain in a time course analysis. Analysis of the RT-PCR products demonstrated that the monacolin K biosynthetic genes in the transformant were expressed to a greater extent than those in the wild-type strain. These results indicated that mokH upregulated the transcription of monacolin K biosynthetic genes and increased monacolin K production.
Value of MR imaging in differentiating benign from malignant soft-tissue masses: study of 95 lesions. (1990)

MR imaging has largely replaced CT as the technique of choice for preoperative staging of patients with soft-tissue masses. Whether MR imaging can be used to differentiate benign from malignant masses is controversial. Our experience suggests that MR imaging often can characterize soft-tissue masses accurately. To evaluate this question further, we studied 95 consecutive lesions (50 benign and 45 malignant). Consecutive cases were selected to simulate our clinical practice. Surgical proof was available for all masses except hematomas, for which clinical follow-up confirmed the diagnosis. MR images were interpreted twice by three radiologists. The first review was accomplished without any clinical history and the second review with clinical history. Reviewers were asked to classify the lesion as benign or malignant on the basis of their clinical knowledge and analysis of MR image features (size, lesion margin, signal homogeneity, and neurovascular or bone involvement). Although interpretation varied somewhat because of the experience of the reviewers, the specificity and accuracy of diagnosis averaged 90% for both benign and malignant lesions. Negative predictive value for malignancy averaged 94% among the three reviewers. MR imaging is the technique of choice for identification and characterization of soft-tissue masses. The nature of the lesion (benign vs malignant) can be determined in the majority of cases.
Water demand of groundwater dependent ecosystems in the Rétköz and in the northern part of the Nyírség (2013)

Estimation of ecological water demand of groundwater dependent ecosystems is essential in quantitative status assessment of groundwater bodies. In the Hungarian River Basin Management Plan a top-down approach was applied that — due to its resolution and data distribution — proved to be inaccurate in case of critical areas. A more sophisticated method was developed in cooperation with the Institute of Ecology and Botany of the Hungarian Academy of Sciences, which — as a bottom-up approach — is appropriate to calculate groundwater demand of habitats more precisely at regional or even local scale. Comparison of the methods is illustrated in the Nyírség-Lónyay and Rétköz groundwater body group.
Locus-Conserved Circular RNA cZNF292 Controls Endothelial Cell Flow Responses (2021)

Together, our data reveal a hitherto unknown role of cZNF292/cZfp292 in endothelial flow responses, which influences endothelial shape.
Antibody Recognition of SIVmac Envelope Peptides in Plasma from Macaques Experimentally Infected with SIV/Mne (1989)

Four stretches of amino acid sequences encoded in conserved HIV-1 env domains and four parallel regions of the SIVmac env (two from gp120 and two from gp41/p32E) were fused to the NH2 terminus of beta-galactosidase by recombinant DNA techniques and used to analyze sera from three macaque species experimentally infected with SIV/Mne. All SIVmac env sequences were recognized by sera from the SIV/Mne-inoculated macaques. Western blot analysis performed with whole SIV/Mne, SIVmac, SIVagm, and HIV-1 antigens and sera from SIV/Mne-infected macaques also demonstrates that SIV/Mne is immunologically more closely related to SIVmac than to SIVagm or to HIV-1. Antibody levels to the gp120 NH2-terminal SIV-88 epitope appear to decrease in the infected Macaca nemestrina with progression of disease, as was also reported for the parallel HIV-1 epitope in HIV-1-infected individuals. Sera from all infected macaques reacted with the p32E-SIV-582 epitope (EKYLEDQAQLNAWGCAFRQVC). High titers to this immunodominant epitope could be detected at least 9 weeks postinfection and at a time when primarily the p28 and p32E antibodies were detectable in Western blots performed with whole disrupted SIV/Mne virus. In the majority of animals, antibody titers of 1:100,000 to SIV-582 develop during the infection and persist until death. Antibody responses to the SIV env epitopes in SIV/Mne-infected macaques thus resemble in many aspects (prevalence and immunogenicity) those observed previously for the corresponding HIV-1 env epitopes in HIV-1-infected humans.
Alpha2-adrenergic receptor-mediated release of lysophosphatidic acid by adipocytes. A paracrine signal for preadipocyte growth. (1998)

In the search for the existence of adrenergic regulation of the autocrine/paracrine function of the white adipose tissue, it was observed that conditioned media from isolated adipocytes or dialysates obtained by in situ microdialysis of human subcutaneous adipose tissue increased spreading and proliferation of 3T3F442A preadipocytes. These effects were amplified when an alpha2-adrenergic agonist was present during the obtention of conditioned media and microdialysates. This alpha2-adrenergic-dependent trophic activity was completely abolished by pretreatment of the conditioned media or microdialysates with the lysophospholipase, phospholipase B. Among the different lysophospholipids tested only lysophosphatidic acid (LPA) was able to induce spreading and proliferation of 3T3F442A preadipocytes. Moreover, previous chronic treatment of 3T3F442A preadipocytes with LPA which led to a specific desensitization of LPA responsiveness, abolished the alpha2-adrenergic-dependent trophic activities of the conditioned media and microdialysates. Finally, alpha2-adrenergic stimulation led to a rapid, sustained, and pertussis toxin-dependent release of [32P]LPA from [32P]-labeled adipocytes. Based upon these results it was proposed that in vitro and in situ stimulation of adipocyte alpha2-adrenergic receptors provokes the extracellular release of LPA leading, in turn, to regulation of preadipocyte growth.
Measurement of laser-ion guiding channel strength (1986)

Particulate matter (PM) is harmful to human health, especially for people with asthma. The goal of this study was to enhance the knowledge about the short-term effects of daily air concentrations of PM on health outcomes among asthma patients. The novelty of this study was the inclusion of a homogeneous group of patients (N = 300) with diagnosed and partly controlled asthma. Patients recorded their symptoms, asthma quick-relief inhaler use, and peak expiratory flow (PEF) measurements in a diary for two weeks. Data on particulate air pollution were obtained from stationary monitoring stations. We have shown that particulate pollutants (PM<sub>10</sub> and PM<sub>2.5</sub>) are associated with significant deterioration of PEF and an increase in the frequency of early asthma symptoms, as well as asthma quick-relief inhaler use. These effects are observed not only on the day of exposure, but also on the following day. For public health practice, these results support the rationale for using peak-flow meters as necessary devices for proper asthma self-management and control, especially in locations where the air is polluted with particles. This may decrease the number of asthma patients seeking medical help.
Online Multitask Learning with Long-Term Memory (2020)

We introduce a novel online multitask setting. In this setting each task is partitioned into a sequence of segments that is unknown to the learner. Associated with each segment is a hypothesis from some hypothesis class. We give algorithms that are designed to exploit the scenario where there are many such segments but significantly fewer associated hypotheses. We prove regret bounds that hold for any segmentation of the tasks and any association of hypotheses to the segments. In the single-task setting this is equivalent to switching with long-term memory in the sense of [Bousquet and Warmuth; 2003]. We provide an algorithm that predicts on each trial in time linear in the number of hypotheses when the hypothesis class is finite. We also consider infinite hypothesis classes from reproducing kernel Hilbert spaces for which we give an algorithm whose per trial time complexity is cubic in the number of cumulative trials. In the single-task special case this is the first example of an efficient regret-bounded switching algorithm with long-term memory for a non-parametric hypothesis class.
Relative information content of gestural features of non-verbal communication related to object-transfer interactions (2013)

In order to implement reliable, safe and smooth human-robot&#13;\nobject handover it will be necessary for service robots to&#13;\nidentify non-verbal communication gestures in real-time. This&#13;\nstudy presents an analysis of the relative information content&#13;\nin the gestural features that together constitute a&#13;\ncommunication gesture. Based on this information theoretic&#13;\nanalysis we propose that the computational complexity of&#13;\ngesture classification, for object handover, can be greatly&#13;\nreduced by applying attention filters focused on static hand&#13;\nshape and orientation.
Development of a 2 D.O.F Food Handling Gripper Achieving Circular Cone Shape Presentation (2016)

In recent years, industrial robots have been applied to food production due to its huge potential market size. Although cooking large amount of foods are done by specially designed cooking machines, dishing up the cooked foods remains as a labor-intensive task. To solve this problem, this paper proposes a new food handling gripper that is suitable for lunch box setout considering appetizing shape of presentation. A two degree-of-freedom multi-fingered gripper with a sliding push part was developed, and evaluated by grasping experiments with noodles and simmered foods. The gripper could put the food with a circular cone shape and control the amount of grasped food.
Exponential Integrators for Large Systems of Differential Equations (1998)

We study the numerical integration of large stiff systems of differential equations by methods that use matrix--vector products with the exponential or a related function of the Jacobian. For large problems, these can be approximated by Krylov subspace methods, which typically converge faster than those for the solution of the linear systems arising in standard stiff integrators. The exponential methods also offer favorable properties in the integration of differential equations whose Jacobian has large imaginary eigenvalues. We derive methods up to order 4 which are exact for linear constant-coefficient equations. The implementation of the methods is discussed. Numerical experiments with reaction-diffusion problems and a time-dependent Schrödinger equation are included.
Pseudarthrosis in Adult Spinal Deformity Following Multisegmental Instrumentation and Arthrodesis (2006)

The prevalence of pseudarthrosis following long arthrodesis with use of modern segmental spinal instrumentation for the treatment of spinal deformity in adults was 17%, and the clinical outcome in these patients can be negatively affected by the pseudarthrosis.