gebhart/patrepeval huggingface dataset:
Schwangerschaftsausgang bei 1252 Feten nach Nackentransparenzmessung im ersten Trimenon (2002)

The measurement of the nuchal translucency is an important marker to detect a vast number of fetal anomalies. Lately the nuchal translucency has been used increasingly as a screening method to find chromosomal anomalies especially for trisomy 21. Beside a high incidence of chromosomal defects one assumes a high risk of rare syndromes and other associated anomalies such as heart defects, skeletal anomalies, cerebral anomalies, diaphragmatic hernias, as well as of an intrauterine death. In view of abortion rate, detection rate of chromosomal anomalies, other fetal anomalies and rare syndromes we evaluated in this study the pregnancy outcome after nuchal translucency measurement, and discuss a concept for its management.
Table S1 from Phase II Trial of Ipilimumab with Stereotactic Radiation Therapy for Metastatic Disease: Outcomes, Toxicities, and Low-Dose Radiation–Related Abscopal Responses (2023)

&lt;p&gt;Patient characteristics&lt;/p&gt;
What Is the Difference between the Nature Eating Rhythm and the Actual Eating Behaviors in Malaysian Adults? A Cross-Sectional Study of Circadian Misalignment (2022)

Eating behavior different from the natural eating rhythm increases the risk of metabolic syndrome. With simply adjusted eating behaviors to line up with the natural rhythms, it prevents the further development of metabolic disorder. To fill the gap in nutritional therapy in metabolic syndrome, this study aimed to determine the contrast between natural eating rhythm and actual eating behaviors in Malaysians. The cross-sectional comprised of a total of 221 participants aged 20 to 63 years old, who completed online survey to collect their sociodemographic characteristics, eating rhythm, sleep patterns and eating behaviors. A validated chrononutrition questionnaire was used as indicator of eating rhythm and practices. Descriptive data showed the mean and standard deviation of the natural eating rhythm and actual eating behaviors in table, while the misalignment of chrononutrition was reported as frequencies on graph. The majority of participants were female (67.0%) and graduated from university (76.5%). They were non-alcohol drinkers (96.8%) or had no smoking history (71.5%). Although the mean for the natural eating rhythm was 08:02 ± 01:26, participants tend to start their first eating occasion during free days (09:11 ± 01:26) later than during work or school days (08:08 ± 00:05). The overall mean eating occasion before bedtime was 23:30 ± 01:54, there were no difference between eating occasion before bedtime during work or school days (19:50 ± 01:10) and free days (19:59 ± 01:20). This study found that 17.6% and 13.1% of the participants had extreme morning latency misalignment during work or school days, and free days respectively. Approximately half of the participants had extreme evening latency misalignment during work or school days and free days, with 35.8% and 48.8% separately. A total mean of 701.48 ± 104.69 minutes spent eating window during work or school days and 646.67 ± 115.71 minutes used during free days. Malaysian adults had differed between their natural eating rhythm and actual eating practices. The results suggested to review circadian misalignment, typically eating behaviors in metabolic regulation in future studies. The researchers appreciated the Ministry of Higher Education of Malaysia for providing a support under the Fundamental Research Grant Scheme (FRGS/1/2021/SKK06/TARUC/02/1).
Dynamically Compiled Artifact Sharing for Clouds (2017)

Platform as a Service (PaaS) clouds provide part of the hardware/software stack and related services to tenant applications. Increased load is handled elastically by scaling, which either modifies the number of instances an application has available on the cloud or increases their available resources. However, because all these instances run inside isolated containers, experience gained by the first instance of an application cannot be easily shared with subsequent scaled instances. This results in both increased startup time and response timeout errors for the scaled instances as well as increased performance interference for any co-located applications; reacquiring this experience is a time-consuming and resource-intensive process. We propose a scalable and secure technique to share dynamically compiled artifacts produced by the first execution instance of an application and otherwise created for intra-OS sharing only with subsequent scaled or restarted instances as a solution to these problems. Our solution abides by the usual PaaS limitations and uses a distributed and containerized cloud service, which we experimentally show to be scalable on a Docker Swarm running on top of a 6-VM cluster; also, we discuss the results of a usability survey for the service's GUI conducted with expert subjects. The effectiveness of the DCAS technique was experimentally tested on an isolated installation of the PaaS software Cloudy Foundry; we measured significant reductions in both the startup time and response errors of scaled out instances as well as performance interference to co-located tenants during scaling.
Photodynamic antisense regulation using psoralen-conjugated oligo(nucleoside phosphorothioate)s (I). Growth regulation of cervical carcinoma cells (1999)

To increase the antisense regulatory effect of oligo(nucleoside phosphorothioate)s (S-Oligo), a photo-crosslinking reagent, 4, 5', 8-trimethylpsoralen, was used in this study. Psoralen-conjugated oligo(nucleoside phosphorothioate) (Ps-S-Oligo) complementary to the human papillomavirus type 18 (HPV18) mRNA drastically inhibited the cellular proliferation of cervical cancer cells only upon UVA-irradiation. In contrast, Ps-S-Oligos with mismatched sequences and scrambled one showed lesser inhibitory effects than that with matched one. These results suggest that psoralen-conjugated antisense S-Oligo has significant potential to regulate gene expression upon UVA-irradiation.
Differential Presentation of Glutamic Acid Decarboxylase 65 (GAD65) T Cell Epitopes Among HLA-DRB1*0401-Positive Individuals (1999)

Glutamic acid decarboxylase 65 (GAD65) is one of the major autoantigens in type 1 diabetes. We investigated whether there is variation in the processing of GAD65 epitopes between individuals with similar HLA backgrounds and whether the processing characteristics of certain immunogenic epitopes are different in distinct APC subpopulations. Using DR401-restricted T cell hybridomas specific for two immunogenic GAD65 epitopes (115-127 and 274-286), we demonstrate an epitope-specific presentation pattern in human B-lymphoblastoid cell lines (B-LCL). When pulsed with the GAD protein, some DRB1*0401-positive B-LCL, which presented GAD65 274-286 epitope efficiently, were unable to present the GAD65 115-127 epitope. However, all B-LCL presented synthetic peptides corresponding to either GAD epitope. In addition, when pulsed with human serum albumin, all cell lines gave equal stimulation of a DR4-restricted human serum albumin-specific T hybridoma. GAD65-transfected cell lines displayed the same presentation phenotype, showing that lack of the presentation of the 115-127 epitope was not due to inefficient uptake of the protein. Blood mononuclear adherent cells, B cells, or dendritic cells derived from the same individual displayed the same presentation pattern as observed in B cell lines, suggesting that the defect most likely is genetically determined. Therefore, individual differences in Ag processing may result in the presentation of distinct set of peptides derived from an autoantigen such as GAD65. This may be an important mechanism for the deviation of the immune response either into a regulatory pathway or into an inflammatory autoimmune reactivity.
Locally isolated yeasts from Malaysia: identification, phylogenetic study and characterization. (2012)

Yeasts are a convenient platform for many applications. They have been widely used as the expression hosts. There is a need to have a new yeast expression system to contribute the molecular cloning demands. Eight yeast isolates were screened from various environment sources and identified through ribosomal DNA (rDNA) Internal Transcribed Spacer (ITS). Full sequence of the rDNA ITS region for each isolate was BLASTed and phylogenetic study was constructed by using MEGA4. Among the isolates, isolate WB from 'ragi' (used to ferment carbohydrates) could be identified as a new species in order Saccharomycetales according to rDNA ITS region, morphology and biochemical tests. Isolate SO (from spoiled orange), RT (rotten tomato) and RG (different type of 'ragi') were identified as Pichia sp. Isolates R1 and R2, S4 and S5 (from the surrounding of a guava tree) were identified as Issatchenkia sp. and Hanseniaspora sp., respectively. Geneticin, 50 µg/mL, was determined to be the antibiotic marker for all isolates excepted for isolates RT and SO which used 500 µg/mL and 100 µg/mL Zeocin, respectively. Intra-extracellular proteins were screened for lipolytic activity at 30°C and 70°C. Thermostable lipase activity was detected in isolates RT and R1 with 0.6 U/mg and 0.1 U/mg, respectively. In conclusion, a new yeast-vector system for isolate WB can be developed by using phleomycin or geneticin as the drugs resistance marker. Moreover, strains RT and R1 can be investigated as a novel source of a thermostable lipase.
Acylcarnitine profiles in serum and muscle of dairy cows receiving conjugated linoleic acids or a control fat supplement during early lactation (2018)

Acylcarnitines (ACC) are formed when fatty acid (FA)-coenzyme A enters the mitochondria for β-oxidation and the tricarboxylic acid cycle through the carnitine shuttle. Concentrations of ACC may vary depending on the metabolic conditions, but can accumulate when rates of β-oxidation exceed those of tricarboxylic acid. This study aimed to characterize muscle and blood serum acylcarnitine profiles, to determine the mRNA abundance of muscle carnitine acyltransferases, and to test whether dietary supplementation (from d 1 in milk) with conjugated linoleic acids (CLA; 100 g/d; each 12% of trans-10,cis-12 and cis-9,trans-11 CLA; n = 11) altered these compared with control fat-supplemented cows (CTR; n = 10). Blood samples and biopsies from the semitendinosus musclewere collected on d -21, 1, 21, and 70 relative to parturition. Serum and muscle ACC profiles were quantified using a targeted metabolomics approach. The CLA supplement did not affect the variables examined. The serum concentration of free carnitine decreased with the onset of lactation. The concentrations of acetylcarnitine, hydroxybutyrylcarnitine, and the sum of short-chain ACC in serum were greater from d -21 to 21 than thereafter. The serum concentrations of long-chain ACC tetradecenoylcarnitine (C14:1) and octadecenoylcarnitine (C18:1) concentrations were greater on d 1 and 21 compared with d -21. Muscle carnitine remained unchanged, whereas short- and medium-chain ACC, including propenoylcarnitine (C3:1), hydroxybutyrylcarnitine, hydroxyhexanoylcarnitine, hexenoylcarnitine (C6:1), and pimelylcarnitine were increased on d 21 compared with d -21 and decreased thereafter. In muscle, the concentrations of long-chain ACC (from C14 to C18) were elevated on d 1. The mRNA abundance of carnitine palmitoyltransferase 1, muscle isoform (CPT1B) increased 2.8-fold from d -21 to 1, followed by a decline to nearly prepartum values by d 70, whereas that of CPT2 did not change over time. The majority of serum and muscle short- and long-chain ACC were positively correlated with the FA concentrations in serum, whereas serum carnitine and C5 were negatively correlated with FA. Time-related changes in the serum and muscle ACC profiles were demonstrated that were not affected by the CLA supplement at the dosage used in the present study. The elevated concentrations of long-chain ACC species in muscle and of serum acetylcarnitine around parturition point to incomplete FA oxidation were likely due to insufficient metabolic adaptation in response to the load of FA around parturition.
Data from Structural Basis for the Inhibition of Human 5,10-Methenyltetrahydrofolate Synthetase by N10-Substituted Folate Analogues (2023)

&lt;div&gt;Abstract&lt;p&gt;5,10-Methenyltetrahydrofolate synthetase (MTHFS) regulates the flow of carbon through the one-carbon metabolic network, which supplies essential components for the growth and proliferation of cells. Inhibition of MTHFS in human MCF-7 breast cancer cells has been shown to arrest the growth of cells. Absence of the three-dimensional structure of human MTHFS (hMTHFS) has hampered the rational design and optimization of drug candidates. Here, we report the structures of native hMTHFS, a binary complex of hMTHFS with ADP, hMTHFS bound with the N5-iminium phosphate reaction intermediate, and an enzyme-product complex of hMTHFS. The N5-iminium phosphate captured for the first time in our crystal structure unravels a unique strategy used by hMTHFS for recognition of the substrate and provides structural basis for the regulation of enzyme activity. Binding of N10-substituted folate analogues places Y152 in the middle of the channel connecting ATP binding site with the substrate binding pocket, precluding the positioning of γ-phosphate for a nucleophilic attack. Using the structures of hMTHFS as a guide, we have probed the role of residues surrounding the active site in catalysis by mutagenesis. The ensemble of hMTHFS structures and the mutagenesis data yield a coherent picture of the MTHFS active site, determinants of substrate specificity, and new insights into the mechanism of inhibition of hMTHFS. [Cancer Res 2009;69(18):7294–301]&lt;/p&gt;&lt;/div&gt;
Time-delayed reservoir computing based on a dual-waveband quantum-dot spin polarized vertical cavity surface-emitting laser (2022)

In this work, we present numerical results concerning a time-delayed reservoir computing scheme, where its single nonlinear node, is a quantum-dot spin polarized vertical cavity surface-emitting laser (QD s-VCSEL). The proposed photonic neuromorphic scheme exploits the complex temporal dynamics of multiple energy states present in quantum dot materials and uses emission from two discrete wavebands and two polarization states, so as to enhance computational efficiency. The benchmark task used for this architecture, is the equalization of a distorted 25Gbaud PAM-4 signal after 50Km of transmission at 1550 nm. Results confirm that although typical ground-state emitting quantum-dot nodes offer limited performance, due to bandwidth limitations; by exploiting dual emission, we achieved a one-hundred-fold improvement in bit-error rate. This performance boost can pave the way for the infiltration of quantum-dot based devices in high-speed demanding neuromorphic driven applications.
Chromosome 8q as the most frequent target for amplification in early gastric carcinoma (2014)

Early gastric carcinoma (GC) is considered to be a curable cancer, as it progresses to the advanced stage following varying durations. Understanding the early stage of GC may provide an insight into its pathogenesis and contribute to reducing the mortality rate of this disease. To investigate the genomic aberrations associated with 22 cases of early GC, high-density microarray comparative genomic hybridization was performed in the present study. The most notable finding was copy number gains (log<sub>2</sub> ratio >0.25) on the long arm of chromosome 8, which occurred in 77.3% (17/22) of GC cases, and the delineated minimal common region was 8q22.1-q24.3. More specifically, two amplified (log<sub>2</sub> ratio >1) loci in the 8q22.1-q24.3 region were detected in 18.2% (4/22) of GC cases. The first loci covered a region of 102.4-107.9 kb, mapping on 8q22.3-q23.1, and comprised the transcription factor CP2-like 3 gene. The second loci, spanning 128.7-145.7 kb on 8q24.21-q24.3, comprised the representative oncogene of myelocytomatosis. Furthermore, the following possible target genes that were not previously considered to play a pathogenic role in GC were identified: Plasmacytoma variant translocation 1, cysteine/histidine rich 1, kinesin family member C2, forkhead box H1, protein phosphatase 1 regulatory subunit 16A, glutamic-pyruvate transaminase, <i>LOC113655</i> and RecQ protein-like 4. In the present study, previous findings showing that 8q mutations accumulate early during the multistage pathogenesis of GC were confirmed and expanded upon. The confirmation of previously reported 8q gains and the identification of novel target genes at 8q22.1-q24.3 amplified chromosomal sites should aid in improving our understanding of the molecular mechanisms underlying the tumorigenesis of early GC.
<title>A laser beam homogenizer with a variable output cross section</title> (2003)

New studies concerning the performance of Pd films embedded with hydrogen are presented. The films were realized on Si wafers by thermal evaporation and soaked in hdyrogen for different days. Every experiment processed two or more samples, one of which was irradiated by an excimer laser the day before its analysis. The laser fluence was lower than 25 mJ/cm<sup>2</sup> in order to avoid the film ablation and the maximum gas pressure was 5 bar. The samples were analyzed by scanning electron microscopy and an electron microanalysis. Just after four processing days the films showed a morphological modification while with more than eight processing days new elements were observed, Na, S, Ca nd Rn. The found elements were absent in the no-treated samples.
Ultrasonic Excitation Induced Wenzel to Cassie Transition (2011)

Wetting on textured solids has gained much attention in the past decade due to increasing interest in artificial superhydrophobic surfaces. (Bahadur &amp; Garimella, 2007; Boreyko &amp; Chen, 2009; Forsberg, Nikolajeff, &amp; Karlsson, 2011; Heikenfeld &amp; Dhindsa, 2008) On textured surfaces, the wetting liquid can be in either the Cassie–Baxter state, which the liquid does not fill the surface texture; or the Wenzel state, which the liquid completely wets the surface and fills the recesses. For a hydrophobic micro-scale rough surface, the Cassie state is usually a more favorable state since it requires less energy. However, due to contact angle hysteresis, the Wenzel state can also be meta-stable. By controlling the roughness of the texture and initial droplet position, both Cassie and Wenzel states can be stable simultaneously. (Koishi, Yasuoka, Fujikawa, Ebisuzaki, &amp; Xiao, 2009) However, with the proper energy input, the droplets can be induced to transition between states. While multiple methods have been developed to switch from Cassie to Wenzel states (Bormashenko, Pogreb, Whyman, &amp; Erlich, 2007; Krupenkin et al., 2007; Kumari &amp; Garimella, 2011; Ran, Ding, Liu, Deng, &amp; Hou, 2008), it is much more difficult to switch from the Wenzel state to the Cassie state. Wenzel-Cassie transitions have been achieved by changing the surface structure to destabilize the Wenzel state (Krupenkin et al., 2007)(Ran et al., 2008) or by changing the ambient fluid. (Dhindsa et al., 2006)
Biocatalysis and Enzyme-Analogous Processes (2002)

3.2.1.1 Introduction 872; 3.2.1.2 Examples of Enzymatic Conversions 873; 3.2.1.3 Enzyme-Analogous Catalysts 886; 3.2.1.4 Commercial Applications 887; 3.2.1.5 Outlook 906
Lösungsmöglichkeiten bei fehlgeschlagenen Osteosynthesen – Humerusschaft (2020)

Zusammenfassung Bei der operativen Therapie von Humerusschaftfrakturen finden sowohl die intramedulläre Nagel- als auch die Plattenosteosynthese regelhaft Anwendung. Für beide Verfahren kann zwischen Früh- und Spätkomplikationen unterschieden werden. Bei der Nagelosteosynthese werden im frühen postoperativen Verlauf häufiger periimplantäre Frakturen etwa am Tuberculum majus oder im Bereich der Fossa olecrani beobachtet. Bei der Plattenosteosynthese hingegen kommt es häufiger zum Repositionsverlust mit sekundärer Dislokation sowie zu Affektionen des Nervus radialis. Pseudarthrosen als klassische Spätfolge zeigen sich häufiger nach der Nagelosteosynthese. Früh- und Spätinfekte im Bereich des Humerusschafts sind insgesamt selten. In der vorliegenden Arbeit werden Lösungsmöglichkeiten bei fehlgeschlagenen Osteosynthesen des Humerusschafts anhand edukativer Fallbeispiele und der aktuellen Literatur dargestellt.
Effect of growth hormone and insulin-like growth factor-I on DNA synthesis and matrix production in rat epiphyseal chondrocytes in monolayer culture (1992)

The influence of various culture conditions was studied on the effect of GH and insulin-like growth factor-I (IGF-I) on DNA and matrix synthesis in epiphyseal rat chondrocytes in monolayer culture. Chondrocytes from enzymatically digested rat tibia epiphyseal growth plates were seeded in 48-well culture plates and precultured for 10 days in Ham's F-12 medium supplemented with 1% (v/v) newborn calf serum and 1% (v/v) of a serum substitute. After preculture, the medium was changed to Ham's F-12 medium supplemented with 1% serum from hypophysectomized rats, and the effect of GH and IGF-I on DNA synthesis ([3H]thymidine incorporation) and matrix production ([35S]sulphate uptake) was studied during an additional 96-h culture period. Isotopes were present during the last 24 h of culture. Both hGH and IGF-I stimulated DNA synthesis in a dose-dependent manner. A maximal effect of GH was seen at a concentration of 25 micrograms/l (60 +/- 11% stimulation over control) and for IGF-I at 10 micrograms/l (162 +/- 12%). The stimulatory effects of the same concentrations of human GH (hGH) and IGF-I on [35S]sulphate uptake were 135 +/- 25 and 320 +/- 42% respectively. In-vitro pulse labelling revealed that GH did not produce a response during the first 3 days of culture (after addition of GH) but was effective during days 4 and 5 of culture. In contrast, IGF-I was effective throughout the culture period. Pretreatment of cells with GH or IGF-I for 2.5 days showed that GH but not IGF-I produced a sustained effect on [3H]thymidine uptake. In order to study the influence of cell density on the effect of GH and IGF-I on DNA synthesis, the effect of added peptides was evaluated after different preculture periods (5-15 days). A maximal stimulatory effect of hGH was seen at a cell density of 150,000-300,000 cells/cm2. GH had no significant effect at a low (less than 100,000 cells/cm2) or a high (greater than 400,000 cells/cm2) cell density. The magnitude of the stimulatory effect of IGF-I was the same at densities between 10,000 and 250,000 cells/cm2, but was reduced at higher cell densities (over 250,000 cells/cm2). Chondrogenic properties of cells that had been cultured for 15 days were verified in vitro by positive alcian blue staining and identification of type II collagen, and in vivo by development of cartilage nodules in nude mice.(ABSTRACT TRUNCATED AT 400 WORDS)
Synthesis and structure of novel 4‐arylhexahydro‐1H,3H‐pyrido[1,2‐c]pyrimidine derivatives (1999)

Abstract A series of new 4‐aryl‐hexahydro‐1 H ,3 H ‐pyrido[1,2‐ c ]pyrirnidine‐1,3‐dione derivatives 4a‐k were prepared by catalytic hydrogenation of 4‐aryl‐1 H ,2 H ‐pyrido[1,2‐ c ]pyrirnidine‐1,3‐diones 3a‐k. The structures of compounds were determined by 1 H and 13 C nmr spectroscopy in solution. Steric hindrance caused twisting of the phenyl ring with respect to the pyridopyrimidine system, the effect was confirmed by X‐ray diffraction.