gebhart/patrepeval huggingface dataset:
Microfluidic Impedance‐Deformability Cytometry for Label‐Free Single Neutrophil Mechanophenotyping (2022)

The intrinsic biophysical states of neutrophils are associated with immune dysfunctions in diseases. While advanced image-based biophysical flow cytometers can probe cell deformability at high throughput, it is nontrivial to couple different sensing modalities (e.g., electrical) to measure other critical cell attributes including cell viability and membrane integrity. Herein, an "optics-free" impedance-deformability cytometer for multiparametric single cell mechanophenotyping is reported. The microfluidic platform integrates hydrodynamic cell pinching, and multifrequency impedance quantification of cell size, deformability, and membrane impedance (indicative of cell viability and activation). A newly-defined "electrical deformability index" is validated by numerical simulations, and shows strong correlations with the optical cell deformability index of HL-60 experimentally. Human neutrophils treated with various biochemical stimul are further profiled, and distinct differences in multimodal impedance signatures and UMAP analysis are observed. Overall, the integrated cytometer enables label-free cell profiling at throughput of >1000 cells min<sup>-1</sup> without any antibodies labeling to facilitate clinical diagnostics.
ChemInform Abstract: Photocurrent Spectroscopic Investigations of Passive Films on Chromium. (1990)

Abstract The photoelectrochemical characterization is carried out for passive films electrochemically grown on chromium at potentials at which Cr(III) is the only thermodynamically stable species (i.e.
Chemistry of and with Highly Reactive Metals (1993)

Abstract Today, the synthetic chemist has a large repertoire of metal activation methods at his disposal. After a first breakthrough was made at the beginning of the seventies with the introduction of the Rieke metals, a series of further, in part more efficient methods were describes, based on which not only classical metal‐induced reactions could be substantially improved but also completely new reactions could be discovered. In this article the individual activation methods are discussed and compared as far as is possible using the currently available data. Especially noteworthy are the metal–graphite combinations because of their unsurpassed reactivity and concomitant easy preparation and manipulation. As shown by numerous applications of these reagents on polyfunctional substrates, particularly natural products, high reactivity of the metal and excellent selectivity are by no means precluded. Besides the purely preparative aspects also insights gained so far into the general principles and limits of metal activation are outlined, and attempts at determining the morphology of highly dispersed systems are reported.
Induction of Protective Antitumor Immunity through Attenuation of ERAAP Function (2013)

The endoplasmic reticulum aminopeptidase associated with Ag processing, ERAAP, plays an important role in the trimming of antigenic peptides for presentation at the cell surface complexed with MHC class I molecules. Tumors express varying levels of ERAAP, highlighting a possible mechanism of immune-evasion through alteration of the peptide repertoire. Using the CT26 tumor model, we investigated the effects of ERAAP modulation on peptide presentation and the use of ERAAP inhibition as an antitumor therapy. We show that generation of the cross-protective tumor Ag GSW11 in the colorectal carcinoma CT26 is increased when ERAAP expression is reduced. BALB/c mice with reduced ERAAP expression challenged with CT26 induced protective immunity that was mediated by CD8(+) T cells. This antitumor immunity also protected mice when rechallenged with wild-type CT26 tumor; strong CD8(+) T cell responses to GSW11 were observed, despite its presentation being considerably lower. Furthermore, boosting the tumor immunogenicity through inhibition of ERAAP function with the small molecule inhibitor leucinethiol in vitro, or in established tumors in vivo, abrogated tumor growth and prolonged survival. Thus, our results highlight the promising possibility of using modulation of ERAAP to generate protective antitumor responses as a strategy for cancer immunotherapy.
Decreasing the complexity of deriving test suites against nondeterministic finite state machines (2017)

The paper presents a Finite State Machine (FSM) based approach for deriving tests with reduced complexity, under the White Box testing assumption where all the faulty implementations are explicitly enumerated. The specification and implementation FSMs are assumed to be initialized, i.e., each possibly partial and (non-observable) nondeterministic FSM has a reliable reset. The proposed technique is illustrated by the application to a communication protocol.
Awschalomet al. reply (1993)

Received 24 August 1992DOI:https://doi.org/10.1103/PhysRevLett.70.2199©1993 American Physical Society
Strategic Ambiguity in the Birth of a Loosely Coupled Organization (1993)

The processes surrounding the birth of organizations have been explained on the basis of structural contingency, resource dependency, population ecology, and institutional theories. In general, these theories view the role of communication and interpretation as largely unproblematic. We argue that strategically ambiguous messages play a key role in the birth of loosely coupled organizations. The successful birth is contingent on these messages being interpreted differently by key constituencies in the organization's environment. A case study is presented in support of this argument.
A vortex pump-based optically-transparent microfluidic platform for biotech and medical applications (2007)

This paper reports an automated polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor that demonstrates the detection of specific binding of biomolecules and that qualitatively monitors cell adhesion on the sensor surface. Micropumps, microchannels, and an SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied for its potential applications in bio-molecules detection and drugs discovery. Two experiments, (1) monitoring the reaction between the BSA-BSA antibody, and (2) monitoring the activities of living cells in the presence or absence of trypsin in a RPMI-1640 medium, were conducted to show the biomedical application capability. Because SPR based bio-detection requires optically transparent substrates, PMMA is a potential replacement for glass and silicon-glass in microfluidic systems, if bio-compatibility and low-cost are desired. Hence, our work has shown the feasibility of commercializing an SPR based bio-medical/chemical analysis system in the near future.
Forty years' experience with a special, non-tumorous application of radiotherapy for the eye. (1996)

This paper recapitulates experience made over 40 years with a special nontumorous radiotherapy for the eye. Following a review of the development of therapy, which began with the pendular convergence x-ray machine and later changed to the linear accelerator, an overview is presented of the most important indications for the safe use of x-rays in exudative inflammatory processes in the posterior segment of the eye. We show that in many cases radiotherapy can achieve a permanent improvement for years or at least the preservation of the status quo, while also protecting the important visual cells, especially if it is administered at the earliest possible stage alone. However, it is even more effective in combination with retrobulbar injection therapy that is adjusted individually. Statistics on the results in cases of disciform macular degeneration, doubtlessly the most frequent indication for non-tumorous radiotherapy in the eye, exemplify its efficacy.
Evaluation of the Circulation of Reconstructive Flaps Using Laser- Induced Fluorescence of Indocyanine Green (1999)

A new method for evaluating the circulation in surgical flaps using laser-induced fluorescence of indocyanine green (ICG) images is reported. In clinical trials the authors found that ICG imaging demonstrated good circulation accurately in 16 of 21 flaps with no clinical manifestations of compromised circulation. In 3 patients in whom partial discoloration and cyanosis of the flaps were visible, the dye study indicated poor circulation in the identical areas. In 2 other patients in whom flaps appeared clinically satisfactory, the flaps were shown by ICG imaging to have greatly compromised circulation. In a patient in whom the flap was left in place, slough of almost the entire flap resulted. Another flap with questionable circulation was returned to its original location, where it healed. Thus, while it is a still a new approach and under continual evaluation, the use of ICG fluorescence shows promise as a valuable adjunct to current methods of flap evaluation.
Synthesis of Non-uniformly Pr-doped SrTiO<sub>3</sub> Ceramics and Their Thermoelectric Properties (2015)

We demonstrate a novel synthesis strategy for the preparation of Pr-doped SrTiO3 ceramics via a combination of solid state reaction and spark plasma sintering techniques. Polycrystalline ceramics possessing a unique morphology can be achieved by optimizing the process parameters, particularly spark plasma sintering heating rate. The phase and morphology of the synthesized ceramics were investigated in detail using X-ray diffraction, scanning electron microcopy and energy-dispersive X-ray spectroscopy. It was observed that the grains of these bulk Pr-doped SrTiO3 ceramics were enhanced with Pr-rich grain boundaries. Electronic and thermal transport properties were also investigated as a function of temperature and doping concentration. Such a microstructure was found to give rise to improved thermoelectric properties. Specifically, it resulted in a significant improvement in carrier mobility and the thermoelectric power factor. Simultaneously, it also led to a marked reduction in the thermal conductivity. As a result, a significant improvement (> 30%) in the thermoelectric figure of merit was achieved for the whole temperature range over all previously reported maximum values for SrTiO3-based ceramics. This synthesis demonstrates the steps for the preparation of bulk polycrystalline ceramics of non-uniformly Pr-doped SrTiO3.
Bayesian Inference of Phylogeny and Its Impact on Evolutionary Biology (2001)

As a discipline, phylogenetics is becoming transformed by a flood of molecular data. These data allow broad questions to be asked about the history of life, but also present difficult statistical and computational problems. Bayesian inference of phylogeny brings a new perspective to a number of outstanding issues in evolutionary biology, including the analysis of large phylogenetic trees and complex evolutionary models and the detection of the footprint of natural selection in DNA sequences.
Metals in aquatic systems (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetals in aquatic systemsBruce D. Honeyman and Peter H. SantschiCite this: Environ. Sci. Technol. 1988, 22, 8, 862–871Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August 1988https://pubs.acs.org/doi/10.1021/es00173a002https://doi.org/10.1021/es00173a002research-articleACS PublicationsRequest reuse permissionsArticle Views789Altmetric-Citations277LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Equivalent Wall as a Dynamic Model of a Complex Thermal Structure (1997)

The idea of the "thermally equivalent wall," the plane mul tilayer structure of dynamic characteristics similar to those for complex structure, in which three-dimensional heat flow occurs, is presented. The notion of structure factors is introduced and the conditions they impose on response factors are derived. Examples of complex structures are analyzed. For the same examples, thermally equivalent walls were generated. Response factors for the nominal complex structures were compared with the response factors for equivalent walls. The good accuracy in reproducing the response factors indicates that the "thermally equivalent wall" may be used as a sub stitute of a wall with thermal bridges in building design energy simulations.
The case of the Clumsy kernel (2014)

All benchmarks are wrong until proven otherwise. Benchmarking is
an amazingly error-prone activity, with results commonly misinterpreted
and wrong conclusions drawn. However, every now and then,
a benchmark takes me by surprise and not only is correct but also identifies a
legitimate issue. This is a story of debugging a benchmark.
Sublethal Exposure to UV Radiation Affects Respiration Rates of the Freshwater Cladoceran Daphnia cafawba (2005)

We examined the effects of UV radiation (UVR) on metabolic rates of the freshwater cladoceran Daphnia catawba. We exposed D. catawba to UVB for 12 h in a lamp phototron at levels of 2.08 and 4.16 kJ m(-2) both with and without concomitant exposure to UVA and visible photorepair radiation (PRR). We also included a group that received PRR only and a dark control group. Respiration rates were measured for 6 h following exposure. Respiration rates increased by 31.8% relative to the dark control at the lowest level of UVB stress (2.08 kJ m(-2) UVB with PRR), whereas respiration was inhibited by 70.3% at the highest stress level (4.16 kJ m(-2) UVB without PRR). Survival rates in the group that received PRR only and the group exposed to 2.08 kJ m(-2) and PRR were not significantly different from that in the control group; however, the survival rate was reduced for all other UVR exposures. We hypothesize that enhanced respiration rates reflect energetic costs related to repair of cellular components damaged by sublethal levels of UVR. Increases in respiration rate of the magnitude we found in our experiment could significantly reduce energetic reserves available for growth and reproduction, especially in cases where these costs are incurred repeatedly during a series of days with high levels of UVR.
(1S,2R,3S,6S,7R)-3,7,11,11-Tetramethyl-6,7-epoxybicyclo[5.4.0]undecane-2-ol (2014)

The title compound, C15H26O2, was synthesized from β-himachalene (3,5,5,9-tetra-methyl-2,4a,5,6,7,8-hexa-hydro-1H-benzo-cyclo-heptene), which was isolated from the Atlas cedar (cedrus atlantica). The mol-ecule is built up from a seven-membered ring to which a six- and a three-membered ring are fused. The seven- and six-membered rings each have a twist-boat conformation. In the crystal, O-H⋯O hydrogen bonds link the mol-ecules into zigzag chains running along the b-axis direction.
Program repair that learns from mistakes (2018)

Automated program repair is a very active research field, with promising results so far. Several program repair techniques follow a Generate-and-Validate work-scheme: programs are iteratively sampled from within a predefined repair search space, and then checked for correctness to see if they constitute a repair.
Address space randomization for mobile devices (2011)

Address Space Layout Randomization (ASLR) is a defensive technique supported by many desktop and server operating systems. While smartphone vendors wish to make it available on their platforms, there are technical challenges in implementing ASLR on these devices. Pre-linking, limited processing power and restrictive update processes make it difficult to use existing ASLR implementation strategies even on the latest generation of smartphones. In this paper we introduce retouching, a mechanism for executable ASLR that requires no kernel modifications and is suitable for mobile devices. We have implemented ASLR for the Android operating system and evaluated its effectiveness and performance. In addition, we introduce crash stack analysis, a technique that uses crash reports locally on the device, or in aggregate in the cloud to reliably detect attempts to brute-force ASLR protection. We expect that retouching and crash stack analysis will become standard techniques in mobile ASLR implementations.
Design, manufacturing and packaging of high frequency micro ultrasonic transducers for medical applications (2011)

The challenges for the realization of a miniaturized high frequency ultrasonic transducer linear array lie in the interconnections on the fine pitch piezoceramic elements. Within the footprint the size of a needle, only peripheral interconnections can be allowed on the transducer array such that the acoustic operations on both faces of the vibrating piezoelectric elements are not obstructed. The very low maximum processing temperature allowed also poses difficulty for conventional bonding techniques. This article presents 3-dimensional packaging using spirally rolled flexible circuits, room-temperature anisotropic conductive bonding and stencil printing for the setup of a wafer-level production process flow.
Evaluating the Value of E-Business Enterprises in the View of Knowledge Asset (2010)

This paper,from the theory of intellectual assets,enterprise value and the operational mechanism of enterprises,drawing on the Lev and Sougiannis(1996),Feltham and Ohlson(1995)and Li Xinyuan(2002),comes up with the evaluation model of intellectual assets and the contribution level of assets to the total value of enterprise.Firms whose names are listed in theChina's top 500 E-Business firmsedited by China Electronic Commerce Association in 2004 are selected.Firms whose share price and book data change unusually are excluded.So finally we obtained 53 companies and the database comes from China Center for Economic Research(CCER)database and companies' own website and the period is between 2005 and 2007.According to empirical results,the contribution of real assets is the greatest and the increase of the investment on marketing assets cannot enhance enterprise value and profit.
Cytokine-Driven Immortalization of in vitro Activated Human T Lymphocytes (1998)

Like other normal human somatic cells, T lymphocytes are believed to have a finite in vitro life span. However, continuous T lymphocyte cell lines can often be established from chronic inflammatory skin diseases when the culture medium is supplemented with IL-2 and IL-4 but without antigen and accessory cells added. Based on the assumption that these continuous T lymphocyte cell lines were activated by antigen during the chronic inflammation taking place in vivo, I investigated whether peripheral blood T lymphocytes could be induced to cytokine-dependent continuous growth following antigen activation. Upon allostimulation, peripheral blood CD4+ T lymphocytes reproducibly escape from cellular senescence. These IL-2- and IL-4-dependent continuous T cell lines show high telomerase activity. Withdrawal of either IL-2 or IL-4 results in cell growth arrest concomitant with down-regulation of telomerase activity. When cultured continuously, these CD4+ human T lymphocytes gradually lose expression of CD28.
Precipitation in a Beta-Brass-Fe Alloy (1959)

Single crystal samples of beta-brass containing 0.1% iron were annealed at 300°C and 400°C in order to develop the iron precipitate particles. At intervals during the anneals, magnetic data were obtained and analyzed in terms of the size, shape, orientation, and general magnetic behavior of the precipitate particles. During the earliest annealing stages, superparamagnetic behavior was observed, and particle diameters were calculated from the Langevin relation. On continued annealing the magnetic properties indicated the presence of mixtures of superparamagnetic and single domain particles, and subsequently mixtures of single domain and multidomain particles. Torque curves showed that the particles were single crystals, with iron-like magnetocrystalline anisotropy, oriented with the same crystal habit as the matrix. Single domain behavior was first observed when the particle diameters were of the order of 200 A. Elongation of the particles in the 〈111〉 directions and rotational hysteresis were noted when single domain behavior appeared. The magnetocrystalline anisotropy coefficients calculated from the torque data were reasonably close to those of iron. Maximum remanence was reached before maximum coercive force due to the much larger initial susceptibility of the superparamagnetic as compared to the multidomain particles. This was confirmed by the shapes of the hysteresis loops for the various cases.
Magnetic field alignment of emulsions to produce porous articles (2023)

The use of magnetic fields in the production of porous articles is generally described. Certain embodiments are related to methods of producing porous articles in which magnetic fields are applied to an emulsion to align emulsion droplets. In some embodiments, after the emulsion droplets have been aligned, the emulsion droplets and/or the medium surrounding the emulsion droplets can be removed to leave behind a porous article. According to certain embodiments, polyvinyl alcohol can be used, for example, to stabilize the emulsion droplets and/or bind together components of the porous article. In some embodiments, water-soluble liquid alcohol can be used, for example, to stabilize the suspension of electronically conductive material within a phase of the emulsion.
Stratospheric constituent trends from ER‐2 profile data (1990)

Trend analysis of the AASE ER‐2 profile data reveals an average decrease in N 2 O on potential temperature isentropes which can be attributed to diabatic cooling of inner vortex air. This conclusion is independently supported by radiative transfer computations. Trends in ozone and water vapor over the same period are not consistent with the magnitude of the diabatic descent. After accounting for the diabatic motion (estimated from N 2 O) an additional 0.44±.3%/day average anomalous O 3 decrease above 440 K (∼20 km) is needed to balance the continuity equation. This ozone decrease suggests additional photochemical destruction of ozone in the presence of the high amounts of ClO observed during the mission. A 0.4±0.3%/day average anomalous increase in H 2 O is also observed near 420 K (∼18 km) which may be due to the evaporation of ice crystals falling from higher, colder stratospheric layers.
Vascular Endothelial Growth Factor-B–Deficient Mice Display an Atrial Conduction Defect (2001)

VEGF-B seems to be required for normal heart function in adult animals but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults.
Compensation of the temperature fluctuations in the silicon photomultiplier measurement system (2012)

This paper presents the method for the compensation of the temperature fluctuations in the measurement system based on the Silicon Photomultipliers. The system was created by the authors of the paper. Temperature has very strong influence on the gain of the detectors. Because single photons are detected and the system is very sensitive, its parameters has to be steady during the measurements. Temperature is being stabilized by changeable bias voltage of the detector. This paper describes how the algorithm of compensation has been determined during series of measurements and calculations.