gebhart/patrepeval huggingface dataset:
Domain-specific CCN3 antibodies as unique tools for structural and functional studies (2007)

CCN3 is a member of the CCN family of cell growth and differentiation regulators that play key roles during embryonic development, and are associated with severe human pathologies. The level of CCN genes' expression is of prognostic value in several types of tumors. In the present manuscript, we report the isolation and characterization of a new set of antibodies targeted against each individual module of the human CCN3 protein. The need for module-specific antibodies stemmed from recent reports indicating that the expression of truncated CCN variant proteins was associated with development of cancers. Each of the four CCN3 modules were expressed as GST fusion proteins and used for rabbits immunization. Polyclonal IgGs purified by two rounds of affinity-chromatography specifically detected both the individual CCN3 domains and the full length CCN3 protein expressed in mammalian cell lines and tissues, as well as recombinant full length and truncated CCN3 proteins. The purified module-specific antibodies were successfully used for Western blotting, immunoprecipitation, immunofluorescence and immunocytochemistry. These antibodies permitted the detection of CCN3 proteins under native and denaturing conditions, and confirmed the sublocalisation of CCN3 proteins in the extracellular compartment, at the cell membrane, in the cytoplasm and in the nucleus of positive cells. Immunocytochemistry and Western blotting studies performed with the module-specific antibodies identified truncated CCN3 proteins in kidney tumor samples. The detection of these rearranged variants provides clues for their involvement in tumorigenesis. Therefore, these antibodies constitute unique tools for the identification of truncated CCN3 proteins in human tissues and may be of great interest in molecular medicine.
The Hippo transducers TAZ and YAP in breast cancer: oncogenic activities and clinical implications (2015)

The Hippo signalling is emerging as a tumour suppressor pathway whose function is regulated by an intricate network of intracellular and extracellular cues. Defects in the signal cascade lead to the activation of the Hippo transducers TAZ and YAP. Compelling preclinical evidence showed that TAZ/YAP are often aberrantly engaged in breast cancer (BC), where their hyperactivation culminates into a variety of tumour-promoting functions such as epithelial-to-mesenchymal transition, cancer stem cell generation and therapeutic resistance. Having acquired a more thorough understanding in the biology of TAZ/YAP, and the molecular outputs they elicit, has prompted a first wave of exploratory, clinically-focused analyses aimed at providing initial hints on the prognostic/predictive significance of their expression. In this review, we discuss oncogenic activities linked with TAZ/YAP in BC, and we propose clinical strategies for investigating their role as biomarkers in the clinical setting. Finally, we address the therapeutic potential of TAZ/YAP targeting and the modalities that, in our opinion, should be pursued in order to further study the biological and clinical consequences of their inhibition.
Exome sequencing in single cells from the cerebrospinal fluid in multiple sclerosis (2014)

More robust methods applied to larger numbers of cells will be needed to define the role of somatic mutations.
Flexibility of the zeolite RHO framework; relocation of cadmium accompanying transformation of the unit cell at high temperatures (1991)

Upon heating Cd2+-exchanged zeolite RHO, a relocation of the extra-framework cation accompanies the transformation from the acentric (A) to centric (C) structure type; this relocation from the eight ring to a six ring site, which are 5.7 Å apart, is the largest observed in a zeolite.
Conductivity measurements of explosively shocked aluminum and OFHC copper used for armature material in a magnetic flux compression generator (2004)

Modeling and characterization of a magnetic flux compression generator (MFCG) requires detailed knowledge of the changes in conductivity of the MFCG materials during the shock-loading phase. In the studies reported here, a thin metallic strip is shocked with an explosively generated shock wave produced from a charge of composition C-4. The shock wave is intended to simulate the shock wave and pressures produced in MFCG research currently being conducted at Texas Tech University. These pressures are estimated to be between 1 and 3 GPa. The experimental setup is arranged so that the shapes of the metallic strip and shock front are the same, as confirmed using optical fibers. This was to ensure that the test sample was shocked uniformly. The metallic test strip is pulsed with a 70 A current pulse during application of the shock wave. The current and voltage across the test sample are measured directly to determine the change in conductivity. Pressure measurements are conducted in separate tests under similar conditions using strain gauges. The results are then compared to results determined previously using a split Hopkinson pressure bar apparatus (SHPB).
Combined radar and illuminator for sea skimmers (CRISS) (2002)

A combined radar and illuminator provides surface combatants with defensive capability against low observable (LO), low flying threats out to the horizon. The key element of this multifunction radar is a four face phased array antenna suite mounted on mechanically stabilized pedestals to correct for ship's pitch and roll and electronically scanned along the horizon dimension only, providing the required high data rates at relatively low cost. Each of the four faces has two apertures for independent transmission of the pulse radar and illumination modes. The radar is a medium PRF pulse Doppler system and the illuminator uses GaAs MMIC modules distributed behind the array aperture to provide CW or ICW illumination modes. Both apertures are combined for the radar receive function providing maximum gain and improved height accuracy. Operating as an autonomous automatic fire control radar, this system engages the sea skimmer threats and off loads the task of close-in quick-reaction defense from the ship's volume radars.
Gene Regulation Networks for Modeling Drosophila Development (1999)

This chapter will very briefly introduce and review some computational experiments in using trainable gene regulation network models to simulate and understand selected episodes in the development of the fruit fly, Drosophila Melanogaster.
Pain and nausea observations have improved our care (2008)

David Mathers says the assessment of pain should be adopted as the fifth vital sign to help nurses give a more compassionate service (letters July 23).
Hepatoprotective effects ofPortulaca oleraceaextract against CCl4-induced damage in rats (2014)

These results demonstrate that purslane exerts protective effects against CCl4-induced damage in rat liver and supports a potential therapeutic use of purslane as an alternative for patients with liver diseases.
Best practice and future challenges for vaccination against porcine circovirus type 2 (2014)

Porcine circovirus type 2 (PCV2)-systemic disease (SD) (initially named as postweaning multisystemic wasting syndrome) was discovered as an occasional disease affecting postweaning pigs in North-America by mid-1990s. Soon afterward, it was noticed as a devastating disease worldwide. Such scenario prompted to develop vaccine prototypes that worked fairly well under experimental conditions. In spite of the multifactorial nature of the PCV2-SD, the first commercialized vaccines containing inactivated or chimeric PCV2 viruses or PCV2 Cap protein represented by far the best system to control the disease under farm conditions. Moreover, vaccination of non-clinically affected pigs demonstrated a significant improvement of average daily weight gain and, in consequence, the economic importance of the PCV2-subclinical infection. In the present review, a comprehensive overview on PCV2 vaccines and best practices on PCV2 vaccination strategies are presented and discussed.
Identification of Campylobacter pylori in gastric biopsy smears. (1990)

The use of gastric biopsy imprint smears to diagnose Campylobacter pylori was compared with the use of tissue sections and cultures. Multiple gastric biopsies were taken from the mucosa of 42 patients during endoscopy. Imprint smears were prepared from the samples used to make tissue sections; other samples were used for microbiologic culture. There was a good concordance (93%) between the morphologic diagnosis of C pylori in the air-dried, Giemsa-stained smears and the tissue sections; the cytologic preparations were clearly positive in six cases (14%) whose sections contained low numbers of the organisms. There was a concordance of 83% between the combined morphologic techniques and the bacteriologic culture. Six positive cases were detected only by the morphologic techniques while one positive case was detected only by bacteriologic culture. C pylori was identified in one or more preparations of the antral biopsy specimens in 23 (55%) of the 42 cases, including 23 (74%) of the 31 cases with a final diagnosis of gastritis or ulcer. These results show the usefulness of the cytologic study of gastric biopsy smears in diagnosing C pylori infections.
Automatic track tracing in SAR CCD images using search cues (2012)

In this paper, we present an algorithm for automatic vehicle track tracing in synthetic aperture radar coherent change detection (SAR CCD) images using search cues. The framework consists of two main steps. The first step uses a rotating matched filter that is modeled to characterize the appearance of vehicle tracks in SAR CCD imagery. For every pixel, the algorithm searches the orientations of the filter that best match the local orientations of the track path using normalized cross correlation. The second step includes track tracing from the estimated orientation image obtained from the previous step. Given a search cue, the tracing algorithm aims to find a parallel track path that maximizes the global length of the curve, and minimizes the differences in the pixel positions and orientations.
Distinct Roles of Opioid and Dopamine Systems in Lateral Hypothalamic Intracranial Self-Stimulation (2016)

The absence of µ-opioid receptor or dopamine transporter did not lead to the absence of intracranial electrical stimulation responsiveness but rather differentially altered it. The present results in µ-opioid receptor knockout mice are consistent with the suppressive involvement of µ-opioid receptors in both positive incentive motivation associated with intracranial electrical stimulation and negative incentive motivation associated with depressive states. In contrast, the results in dopamine transporter knockout mice are consistent with the involvement of dopamine transporters in positive incentive motivation, especially its persistence. Differences in intracranial electrical stimulation in µ-opioid receptor and dopamine transporter knockout mice underscore the multidimensional nature of reward.
Altered red cell membrane fluidity during schizogonic development of malarial parasites (Plasmodium falciparum and P. lophurae) (1984)

The plasma membranes of human or duckling erythrocytes infected with malarial parasites (Plasmodium falciparum and P. lophurae respectively) were stained by the fluorescent dye merocyanine 540 in the presence of serum. Unparasitized erythrocytes from infected ducklings or from in vitro cultures remained unstained in the presence of serum. Because merocyanine 540 has a greater affinity for fluid phased or disordered lipid bilayers the results suggest that upon infection of the red blood cell the erythrocyte plasma membrane becomes disordered or is increased in its fluidity. Such alterations of the host erythrocyte are probably due to parasite-induced modifications in the underlying spectrin network (required for lipid leaflet asymmetry) as well as changes in erythrocyte membrane lipid composition.
Nodular Fasciitis of the Hand (2001)

Nodular fasciitis is a benign, reactive fibroblastic soft tissue tumor. Involvement of the hand is exceedingly rare. A case of nodular fasciitis involving the dominant hand of a 38-year-old woman is reviewed, and its clinicopathologic features are presented. Because of its rapid growth and aggressive histologic appearance, nodular fasciitis can be mistaken for a soft tissue sarcoma, despite its benign clinical behavior. For this reason, aggressive surgical resection should be avoided.
ARC: A camcopter based mine field detection system (2001)

The area affected with antipersonnel land mines in 56 infested countries is estimated to be 1 Mio km. This represents a serious threat to the human lives and a major obstacle for the development of the polluted region. The objective of ARC is to develop, demonstrate and promote a new system for performing the UN Level 2 surveys allowing a quick reduction of suspected mine-polluted areas and post- cleaning quality control. The ARC-system is based on a Camcopter UAV, optical, infrared and hyperspectral sensors and a Mine Information System supported by a Geographical Information System. The sensor data will be processed to extract mine field indicators. The sensor fusion module will combine the mine field indicators with the GIS information to achieve optimal minefield delineation. It is our intention to have the ARC system as soon as possible operational in the field which is supported by early field tests and an evolutionary development path. The project will lead to a demonstration of the system in a mine affected area in Croatia showing its usefulness to potential end users.
Hydroentangled Polymer-Glass Bi-Layer Fibrous Composites (2012)

Hydroentangling (bonding of fabrics by means of high velocity water jets), is utilised to entangle polyethylene terephthalate (PET) and glass fibre layers which are industrially applied as high performance carriers for bitumen roofing products. Conventionally, a resin post-treatment is frequently applied to strengthen pre-entangled PET/glass fabrics whereas in the current study promising mechanical properties were obtained without the need for resin treatment. Non-destructive characterisation using x-ray microtomography reveals the transverse structure of these mechanically bonded fabrics. Normal procedures for measuring delamination cannot be applied to the evaluation of hydroentangled fibrous composites.
Color calibration and color-managed medical displays: does the calibration method matter? (2010)

Our laboratory has investigated the efficacy of a suite of color calibration and monitor profiling packages which employ a variety of color measurement sensors. Each of the methods computes gamma correction tables for the red, green and blue color channels of a monitor that attempt to: a) match a desired luminance range and tone reproduction curve; and b) maintain a target neutral point across the range of grey values. All of the methods examined here produce International Color Consortium (ICC) profiles that describe the color rendering capabilities of the monitor after calibration. Color profiles incorporate a transfer matrix that establishes the relationship between RGB driving levels and the International Commission on Illumination (CIE) XYZ (tristimulus) values of the resulting on-screen color; the matrix is developed by displaying color patches of known RGB values on the monitor and measuring the tristimulus values with a sensor. The number and chromatic distribution of color patches varies across methods and is usually not under user control. In this work we examine the effect of employing differing calibration and profiling methods on rendition of color images. A series of color patches encoded in sRGB color space were presented on the monitor using color-management software that utilized the ICC profile produced by each method. The patches were displayed on the calibrated monitor and measured with a Minolta CS200 colorimeter. Differences in intended and achieved luminance and chromaticity were computed using the CIE DE2000 color-difference metric, in which a value of &utri;E = 1 is generally considered to be approximately one just noticeable difference (JND) in color. We observed between one and 17 JND's for individual colors, depending on calibration method and target.
Coping with qubit leakage in topological codes (2013)

Many physical systems considered promising qubit candidates are not, in fact, two-level systems. Such systems can leak out of the preferred computational states, leading to errors on any qubits that interact with leaked qubits. Without specific methods of dealing with leakage, long-lived leakage can lead to time-correlated errors. We study the impact of such time-correlated errors on topological quantum error correction codes, which are considered highly practical codes, using the repetition code as a representative case study. We show that, under physically reasonable assumptions, a threshold error rate still exists; however, performance is significantly degraded. We then describe simple additional quantum circuitry that, when included in the error detection cycle, restores performance to acceptable levels.
Binding reactivity of C-reactive protein for polycations. (1980)

Abstract C-reactive protein (CRP) is a homogeneous acute phase protein known to exhibit calcium-dependent binding with phosphate monoesters, reacting most avidly with phosphocholine moieties; as a consequence of such interactions CRP can lead to reactions of agglutination, precipitation, and activation of the complement (C) system. Previous studies have shown that protamine sulfate and various naturally occurring and synthetic polymers also lead to activation of the C system when interacted with CRP in the presence of fresh human serum. The present investigation tested directly the possibility that polycations thus represent an additional binding specificity for CRP, using nephelometry in dilute salt solutions as an assay system. CRP, as compared to multiple other serum proteins including immunoglobulins, α-1 acid glycoprotein, amyloid P-component and fibrinogen, was found to aggregate preferentially with protamine sulfate. In contrast to the other known interactions of CRP including that with pneumococcal C-polysaccharide (CPS), this precipitation was found to occur in the absence of calcium. Multiple additional polycations, including polymers of l-lysine, l-arginine, and l-ornithine, similarly aggregated with CRP. Low concentrations of phosphocholine, but not of tetra-l-lysine (TLL), induced marked inhibition of CPS-initiated aggregation of CRP, whereas TLL, but not phosphocholine, induced substantial inhibition of poly-l-arginine-initiated aggregation of CRP, suggesting reactivity at distinct binding sites. However, both small molecules inhibited PLL- and protamine-induced aggregation of CRP, suggesting that these binding sites are closely approximated. These studies provide direct evidence for a discrete calcium-independent polycation binding site on the CRP molecule, which probably is distinct from, but proximal to, that site utilized for the binding of CPS.
Surface Mounted IC Packages—Their Attachment and Reliability on PWBs (1984)

The hermetically sealed ceramic chip carrier package and the plastic small outline (SO) IC package are both now widely used in hybrid microelectronics. These packages are currently being applied with other discrete surface mount devices to PWB technology in order to increase circuit density and reduce weight. This paper discusses the evaluation of two soldering techniques for the attachment of the chip carrier and SOIC packages to epoxy glass PWBs: the first by solder cream printing or pretinning of the circuit board and subsequent attachment by solder reflow, and the second by a novel jet soldering technique. The thermally induced expansion mismatch between epoxy glass and ceramic chip carriers and the strain induced fatigue this causes in the solder joints are now well documented, and results are presented for the effects of temperature cycling ceramic chip carriers soldered onto PWBs. Various PWB materials have been assessed including FR4, elastomer coated FR4, polyimide kevlar, and epoxy glass laminated copper clad invar. Reliability of these assemblies is discussed in terms of the appearance of micro‐cracks in the solder fillets and the occurrence of electrical discontinuity in the solder joints during temperature cycling.
Polypyrrole actuators as valves for controlled drug delivery (2004)

A controlled drug delivery system in which drug release is achieved by actuating an array of polymeric valves on a set of drug reservoirs is introduced. The valves are bilayer structures, with one layer a thin film of evaporated gold and the other electrochemically deposited polypyrrole. The valves are made in the shape of flaps fixed on one side to the valve seats. Drug reservoirs are covered by an array of such valves, and release of the drugs stored in the reservoirs is accomplished by bending the bilayer flaps back with a small applied bias. The fabrication procedures and proof-of-principle drug release experiments for this controlled drug delivery device are described. Energy consumption of this reversible valve design is compared with metal corrosion based valves developed earlier by other and our group.
Pedestrian navigation with foot-mounted inertial sensors in wearable body area networks (2014)

Recent advances in pedestrian navigation, a branch of wearable body area networks (WBAN), facilitate people's lives to help them get access to location-based services (LBS) at anywhere easily. This paper proposes a pedestrian dead-reckoning (PDR) algorithm to track the trajectory of the person using foot-mounted inertial measurement unit (IMU) and the estimation of the foot orientation with extended kalman filter (EKF). The proposed PDR algorithm involves several algorithms: gait phases detection algorithm, orientation estimation algorithm and EKF algorithm with zero velocity update technique (ZUPT). Numerical experiments demonstrate that the proposed algorithm is capable of estimating the trajectory accurately with low computational complexity and estimation error.
Real Time Production Monitoring System (2009)

A production monitoring system is a system that is used in real time to record
production line problems. It is constructed using programmable logic controller and
sensors to collect data from production lines. The real time production monitoring
system is designed to collect data automatically and display the data on display
boards for the workers to see. Devices in the real time production monitoring system
are capable of working as individual units or working together with multiple terminal
links such as automated machineries, robotic systems and manual process line. The
data will also be relayed to the management for prompt action.
Data on production time, non-production time, targets, rejection rates, planned
stops, and cycle times will be displayed as well as relayed to the management.
Unadulterated data collected from the real time production monitoring system should
be relayed to the management without human intervention. Such data would then be
interpreted accordingly in order to identifY the faults at production level and to spur
corrective measures from the management. It is also preferable to link the data
collected to a computer system. With the help of this real time production monitoring
system the management will be able to monitor both the workers and machine
performance. Display of data can also prompt instant action from both workers and
line supervisors.
The data collected should be in line with the requirements of overall equipment
effectiveness. Overall equipment effectiveness is the tool for improving efficiency.
Efficiency of production lines enables better yield and utilization of the available
resources. The real time production monitoring system together with overall
equipment effectiveness will help companies to generate higher revenue.