gebhart/patrepeval huggingface dataset:
Circulating tumor-derived mutant mitochondrial DNA: a predictive biomarker of clinical prognosis in human squamous cell carcinoma (2012)

While circulating tumor-derived molecules have been identified in a variety of malignant tumors, it is sometimes difficult to detect the molecular targets due to the lower serum concentration. We report that evaluation of circulating tumor-derived mitochondrial DNA (mtDNA) seems to have novel efficiency for detecting tumoral micrometastasis. In murine xenografting human oral cancer cells, human mtDNAs could be quantitatively detected from multiple organs and blood samples whereas human nucleic DNAs could not. We also determined if this mtDNA blood test was relevant for patients with oral cancer with no histologic evidence of tumoral cells in their surgical margins. For this, mtDNA from normal and tumorous tissues and serum mtDNA obtained pre and postoperatively was examined at three different regions including the displacement loop, 12S-rRNA, and 16S-rRNA. All non-recurring patients had significantly higher amounts of mutant mtDNAs in the tumoral tissues compared with the non-recurring group. More importantly, on the blood test with the cut-off point by receiver operating characteristic (ROC) curve analysis, while the vast majority of serum mtDNA samples obtained postoperatively in the recurring cases maintained significantly higher amounts of mutant mtDNAs, the non-recurring cases did not, and they showed good prognosis. This is the first report of this approach for managing patients after resection of oral tumors, and may have substantial diagnostic potential for other tumoral types.
Molecular Characterization of Nontypeable Group B Streptococcus (2006)

Traditionally, the capsular polysaccharide (CPS) antigen has been used to distinguish between the nine known serotypes of group B streptococcus (GBS) by classical antibody-antigen reactions. In this study, we used PCR for all CPSs and selected protein antigens, multilocus sequencing typing (MLST), and pulsed-field gel electrophoresis (PFGE) to molecularly characterize 92 clinical isolates identified as nontypeable (NT) by CPS-specific antibody-antigen reactivity. The PCR and MLST were performed on blinded, randomly numbered isolates. All isolates contained the cfb gene coding for CAMP factor. While most (56.5%) contained a single CPS-specific gene, 40 isolates contained either two or three CPS-specific genes. Type V CPS-specific gene was present in 66% of the isolates, and all serotypes except types IV, VII, and VIII were represented. Most (44.5%) of the isolates contained a single protein antigen gene (bca, bac, rib, alp1, or alp3), and the remaining isolates had multiple protein antigen genes. Of the 61 isolates that had the V CPS-specific gene, 48 (78.6%) had the alp3 gene. PFGE analysis classified the isolates into 21 profile groups, while MLST analysis divided the isolates into 16 sequence types. Forty-two (69%) of 61 isolates with the V CPS-specific gene were in PFGE profile group 4; 41 of these 42 were sequence type 1 by MLST. These data shed new light on the antigenic complexity of NT GBS isolates, information that can be valuable in the formulation of an effective GBS vaccine.
Hydrolysis of thioester analogs by rat liver phospholipase A1. (1988)

The hydrolysis of thioester containing phospholipids by rat liver plasmalemma phospholipase A1 was measured in a continuous spectrophotometric assay. In this assay thioester substrates were employed which, upon hydrolysis, liberated a free thiol which was reacted with 4,4'-dithiopyridine to yield the product 4-thiopyridone that absorbs at 324 nm. Thioester substrates, prepared by chemical synthesis, were used in phospholipid and Triton X-100 micelles for kinetic analysis carried out according to the method of Hendrickson and Dennis (Hendrickson, H.S., and Dennis, E.A. (1984) J. Biol. Chem. 259, 5734-5739). Vmax, Ks, and Km values obtained for various isomers and racemic mixtures of the synthetic thioester analogs are compared with corresponding oxyester substrates. Unnatural sn-1 isomers competitively inhibited the hydrolysis of natural sn-3 isomers of phosphatidylethanolamine and phosphatidic acid. Furthermore, the sn-1 isomer of phosphatidic acid was hydrolyzed by phospholipase A1, but with lower catalytic efficiency than the sn-3 isomer. The presence of a thioester at the sn-1 position did not change the Vmax significantly, as compared to the oxyester phospholipids. When two thioesters were present on the phospholipid molecule, the Vmax was decreased significantly. A convenient synthesis of 1-monothioester analogs of phospholipids is reported. The results presented show the usefulness of the spectrophotometric assay for measuring phospholipase A1 activity as well as the influence of racemic mixtures and thioesters on the hydrolytic rate.
Arabidopsis SPA proteins regulate photoperiodic flowering and interact with the floral inducer CONSTANS to regulate its stability (2006)

The four-member SPA protein family of Arabidopsis acts in concert with the E3 ubiquitin ligase COP1 to suppress photomorphogenesis in dark-grown seedlings. Here, we demonstrate that SPA proteins are, moreover, essential for photoperiodic flowering. Mutations in SPA1 cause phyA-independent early flowering under short day (SD) but not long day (LD) conditions, and this phenotype is enhanced by additional loss of SPA3 and SPA4 function. These spa1 spa3 spa4 triple mutants flower at the same time in LD and SD, indicating that the SPA gene family is essential for the inhibition of flowering under non-inductive SD. Among the four SPA genes, SPA1 is necessary and sufficient for normal photoperiodic flowering. Early flowering of SD-grown spa mutant correlates with strongly increased FT transcript levels, whereas CO transcript levels are not altered. Epistasis analysis demonstrates that both early flowering and FT induction in spa1 mutants is fully dependent on CO. Consistent with this finding, SPA proteins interact physically with CO in vitro and in vivo, suggesting that SPA proteins regulate CO protein function. Domain mapping shows that the SPA1-CO interaction requires the CCT-domain of CO, but is independent of the B-box type Zn fingers of CO. We further show that spa1 spa3 spa4 mutants exhibit strongly increased CO protein levels, which are not caused by a change in CO gene expression. Taken together, our results suggest, that SPA proteins regulate photoperiodic flowering by controlling the stability of the floral inducer CO.
International NDT Technical Collaboration Using the Internet (1996)

This paper reviews the capability of the Internet as a world-wide NDT communication tool for technical collaboration, dissemination of information and international standards. This paper was written by authors that never personally met (except for two) and despite thousands of miles distance between them the authors managed to work as a team to complete this paper in a very time efficient manner. This success is attributed to the power of the Internet and it represents a simple example of the.
Microbial Degradation of Perchlorate: Principles and Applications (2003)

Perchlorate (ClO4-) release into the environment has occurred primarily in association with its manufacture and use in solid rocket propellant. When released into groundwater, perchlorate can spread over large distances because it is highly soluble in water and adsorbs poorly to soil. Two proven techniques to remove perchlorate from drinking water are anaerobic biological reactors and ion exchange. In this review, we focus on the application of microbiological systems for degrading perchlorate. Some bacteria can use perchlorate as an electron acceptor while oxidizing a large range of substrates. Perchlorate-respiring bacteria (PRB) are widely distributed in the environment, and are enriched at perchlorate-contaminated sites. We review the pathways by which PRB degrade perchlorate, and the different biological treatment processes that have been developed to remove perchlorate from water sources. We also discuss the effects of alternate electron acceptors in the water, such as oxygen and nitrate, on perchlorate removal. Although many different biological treatment systems using PRB have so far only been proven at the bench scale, all pilot scale tests performed to date with a few of these systems have been successful. The success of these perchlorate bioreactor tests indicates that biological treatment is a suitable method for soil remediation and water treatment of perchlorate-contaminated water.
Identification of Antibiotics That Diminish Disease in a Murine Model of Enterohemorrhagic Escherichia coli Infection (2020)

Infections with enterohemorrhagic <i>Escherichia coli</i> (EHEC) cause disease ranging from mild diarrhea to hemolytic-uremic syndrome (HUS) and are the most common cause of renal failure in children in high-income countries. The severity of the disease derives from the release of Shiga toxins (Stx). The use of antibiotics to treat EHEC infections is generally avoided, as it can result in increased <i>stx</i> expression. Here, we systematically tested different classes of antibiotics and found that their influence on <i>stx</i> expression and release varies significantly. We assessed a selection of these antibiotics <i>in vivo</i> using the <i>Citrobacter rodentium</i> ϕ<i>stx</i><sub>2dact</sub> mouse model and show that <i>stx</i><sub>2d</sub>-inducing antibiotics resulted in weight loss and kidney damage despite clearance of the infection. However, several non-Stx-inducing antibiotics cleared bacterial infection without causing Stx-mediated pathology. Our results suggest that these antibiotics might be useful in the treatment of EHEC-infected human patients and decrease the risk of HUS development.
Nonlinear multi-h phase codes for CPFSK signaling (1995)

Two novel trellis-coding techniques, called nonlinear multi-h signaling and nonlinear asymmetrical multi-h signaling, which achieve higher constraint length than ordinary multi-h signals, are introduced with CPFSK signals. In contrast to ordinary multi-h signaling where the modulation index is changed at the end of every symbol interval, nonlinear multi-h signals are constructed by changing the modulation index based on the previous symbol, and the current symbol interval. A class of nonlinear asymmetrical multi-h signals, which change the modulation index based on the current symbol too, is then constructed by extending nonlinear multi-h signals and combining them with asymmetrical multi-h signals. Binary full-response nonlinear multi-h and nonlinear asymmetrical multi-h CPFSK signals are constructed and analyzed. Minimum Euclidean distances and spectral properties of these signals are calculated at different phase states and various selected modulation index patterns. Numerical results indicate that nonlinear multi-h and nonlinear asymmetrical multi-h signals achieve significantly higher distance than other existing multi-h formats reported in the literature.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Phase Detection with Hidden Markov Models for DVFS on Many-Core Processors (2015)

The energy concerns of many-core processors are increasing with the number of cores. We provide a new method that reduces energy consumption of an application on many-core processors by identifying unique segments to apply dynamic voltage and frequency scaling (DVFS). Our method, phase-based voltage and frequency scaling (PVFS), hinges on the identification of phases, i.e., Segments of code with unique performance and power attributes, using hidden Markov Models. In particular, we demonstrate the use of this method to target hardware components on many-core processors such as Network-on-Chip (NoC). PVFS uses these phases to construct a static power schedule that uses DVFS to reduce energy with minimal performance penalty. This general scheme can be used with a variety of performance and power metrics to match the needs of the system and application. More importantly, the flexibility in the general scheme allows for targeting of the unique hardware components of future many-core processors. We provide an in-depth analysis of PVFS applied to five threaded benchmark applications, and demonstrate the advantage of using PVFS for 4 to 32 cores in a single socket. Empirical results of PVFS show a reduction of up to 10.1% of total energy while only impacting total time by at most 2.7% across all core counts. Furthermore, PVFS outperforms standard coarse-grain time-driven DVFS, while scaling better in terms of energy savings with increasing core counts.
Wireless Dosimeter: System-on-Chip Versus System-in-Package for Biomedical and Space Applications (2008)

A new floating-gate (FG) MOSFET based wireless dosimeter system-in-package (SiP) is presented. This miniature and completely integrated wireless dosimeter SiP comprises a CMOS FG radiation sensor and transmitter (TX) in a low-temperature co-fired ceramic (LTCC) package. The design is very well suited to wireless transmission of radiation sensor data in radiotherapy and to Extra Vehicular Activity Radiation Monitoring (EVARM) in space. Two different solutions, namely system-on-chip (SoC) and SiP, are demonstrated. In the SoC, which is size and power efficient, the TX includes an on-chip loop antenna which also acts as the inductor for the VCO resonant tank circuit. The SiP solution has an LTCC antenna with optimized impedance to conjugate match the TX chip. The radiation sensor demonstrates a measured sensitivity of 5 mV/rad. The SoC module size is only 2 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , consumes 5.3 mW of power and delivers -0.9 dBm of radiated power, sufficient to communicate with a low noise receiver connected to an off-chip patch antenna placed 1.38 m away. The SiP design provides a larger communication range of 75 m at the cost of additional power consumption and size.
Internationalization of MBA Program in Asia-Pacific (1997)

Abstract With the global village becoming a reality, efforts at the internationalization of business education have ceased to be merely fashionable. The internationalization of business education has become an increasing relevant and important goal for business schools. Most of the efforts have been focused at the internationalization of MBA programs as this is the level of business education targeted at the training of middle management personnel, for whom the creation of a global mindset and the exposure to the international realm of business would have the most immediate effect. With the Asia-Pacific becoming a major arena of international trade and business in what has been called the Pacific century, the internationalization of MBA programs in Asia is of great interest as most of the efforts at internationalization of MBA programs have taken place in developed countries, with much of the literature being about the North America and European experience. Little is known about the MBA programs offered in Asia and their internationalization. This paper provides some insights on the internationalization efforts at the Nanyang Business School (NBS), Singapore. The experience at the NBS is unique because its efforts at internationalization have coincided with government policy initiatives in encouraging Singapore businesses to extend their operations into countries of the region. The paper provides some lessons from the experiences at regionalizing the MBA programs in China, India and the transitional economies of Vietnam, Myanmar and Cambodia. Apart from the admission of international participants to its programs and the changes to its Business Study Missions, new initiatives have been introduced in the form of the MBA in International Business. The paper also explores other future initiatives for the internationalization of MBA programs in Asia-Pacific.
The clinical outcome of 50% reduction of low density lipoprotein-cholesterol in patients with acute myocardial infarction: a propensity score matched analysis (2013)

Background: There is a limited data that clinical outcome of 50% reduction of LDL-C from the baseline (at admission) in patient with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI). We investigated whether 50% reduction of LDL-C levels achievement in patients with AMI showed better clinical outcome or not. Methods: This study enrolled 13,473 AMI patients in a large-scale, prospective, multicenter Korean Acute Myocardial Infarction Registry (KorMI) between May 2008 and Sep 2012. A total of 12,720 patients survived and 6,746 patients completed 1-year clinical follow up. Among them 3,315 patients received LDL-C follow up. Propensity score matching was applied to adjust for differences in baseline clinical and angiographic characteristics, producing a total of 1,090 patients (545 half (50%) reduction of LDL-C achievers, vs. 545 nonachievers). The primary end point was the composite of 1-year major adverse cardiac events (MACEs) including cardiac death, recurrent myocardial infarction (MI), target lesion revascularization (TLR) and coronary artery bypass grafting (CABG). Results: After propensity score matching, baseline clinical and angiographic characteristics were similar between the two groups. Clinical outcomes of the propensity score matched patients showed that no significant differences in cardiac death (0.4% vs. 0.6%, P=NS), recurrent MI (0.7% vs. 1.7%, P=NS), TLR (3.7% vs. 4.6%, P=NS), MACEs (5.0% vs. 7.0%, P=NS), and stent thrombosis (2.7% vs. 1.5%, P=NS). Clinical Outcomes upto 1year Conclusions: In this propensity-matched comparison, regardless of absolute LDL-C value, 50% reduction of LDL-C from the baseline in AMI patients did not show the better clinical outcomes.
Buffet loads on Shuttle Thermal Protection System tiles (1981)

This paper presents results of wind tunnel and acoustic tests to investigate buffet loads on Shuttle Thermal Protection System (TPS) tiles. It also describes the application of these results to the prediction of tile buffet loads for the first Shuttle flight into orbit (STS-1). The tests were conducted in wind tunnels at transonic and supersonic Mach numbers and in a progressive wave tube. The wind tunnel tests included single tiles of various thicknesses and arrays of tiles mounted on both rigid and elastic substructures. The corresponding acoustic tests were of the single tiles only. In most cases the “test” tiles were of Orbiter qualified material and they were bonded to the panels and proof-loaded in accordance with flight-vehicle specifications. The fixtures for the panel installations in the wind tunnels were designed to simulate flow regions on the Orbiter where shock waves and related high pressure gradients and high dynamic loads would occur. The results of the wind tunnel tests indicate that significant tile buffet loads occurred due to separated flows and oscillating shocks. Separated flow excitation caused higher buffet loads than oscillating shocks for a given level of excitation, however, the highest combined steady and dynamic loads occurred when shock waves with ΔP &amp;gt; 1.5 psi were on tiles.
A content based video traffic model using camera operations (2002)

We present our work on content based video (CBV) traffic modeling of variable bit rate (VBR) sources. The CBV approach differs from previous works in that it is not based only on matching of various statistics of the original source, but rather on modeling and mapping its visual content into the corresponding bit rate. We show that the CBV model is fully compatible with current and future compression algorithms including those of very low bit rate video coding. We introduce the separation principle between the visual content and encoder dependent bit rate mapping. We construct and verify two experimental CBV models for basic camera operations. The results obtained show that the CBV model can closely match the various statistics of the MPEG-2 VBR stream.
The tomato xylem sap protein XSP10 is required for full susceptibility to Fusarium wilt disease (2010)

XSP10 is an abundant 10 kDa protein found in the xylem sap of tomato. The protein displays structural similarity to plant lipid transfer proteins (LTPs). LTPs are involved in various physiological processes, including disease resistance, and some are able to bind and transfer diverse lipid molecules. XSP10 abundance in xylem sap declines upon infection with Fusarium oxysporum f. sp. lycopersici (Fol), implying involvement of XSP10 in the plant-pathogen interaction. Here, the biochemical characterization of XSP10 with respect to fatty acid-binding properties is reported; a weak but significant binding to saturated fatty acids was found. Furthermore, XSP10-silenced tomato plants were engineered and it was found that these plants exhibited reduced disease symptom development upon infection with a virulent strain of Fol. Interestingly, the reduced symptoms observed did not correlate with an altered expression profile for known reporter genes of plant defence (PR-1 and WIPI). This work demonstrates that XSP10 has lipid-binding properties and is required for full susceptibility of tomato to Fusarium wilt.
Microstructural stability and paint bake response of pre-aged AA7075 (2023)

High strength AA7xxx are attractive for use in the automotive industry, offering significant strength/weight benefits. These alloys are often used in tempers that require long ageing times of several hours. Pre-ageing has been proposed to be effective in suppressing natural ageing, and reducing the total ageing time by integrating the final ageing step into the paint bake cycle. However, the precipitate evolution during the pre-ageing and subsequent paint bake processes remains to be fully understood. In the present work, the pre-ageing process was studied for AA7075 over a wide range of temperatures and times. Small angle X-ray scattering (SAXS) was used as the main technique to investigate the precipitate evolution during the pre-ageing, natural ageing and paint bake, with the support from transmission electron microscopy (TEM) and isothermal calorimetry. For the first time, the results show that 8 h pre-ageing at 80 °C can produce a microstructure consisting of GP zones with an average radius of approximately ∼0.9 nm, which remains stable up to at least 7 months. After a short paint bake process, 94% of the T6 hardness can be obtained by uniformly distributed precipitates with an average radius of approximately ∼2.6 nm. The final size and strengthening effect of the precipitates after 20 min baking is found to be insensitive to the heating rate, which has not been reported previously. The present results further suggest this process is robust for industrial applications.
Wireless sensor networks: creating ‘smart infrastructure’ (2009)

The deterioration of civil infrastructure is a significant issue throughout the world. To manage infrastructure in a way that ensures safe and efficient operation, managers and engineers require data about its short- and long-term performance. This paper reports on the trial installations of wireless sensor networks in a suspension bridge, slab bridge, rail tunnel and water supply pipeline. Each installation is introduced in terms of hardware, measured parameters, sensors, sampling regimes and installation and operational challenges. Preliminary results from each system are discussed to illustrate the variety of information that can be made available to managers and engineers, and how this information can be utilised and presented.