gebhart/patrepeval huggingface dataset:
Resistance to Pyrrolobenzodiazepine Dimers Is Associated with SLFN11 Downregulation and Can Be Reversed through Inhibition of ATR (2021)

Resistance to antibody-drug conjugates (ADCs) has been observed in both preclinical models and clinical studies. However, mechanisms of resistance to pyrrolobenzodiazepine (PBD)-conjugated ADCs have not been well characterized and thus, this study was designed to investigate development of resistance to PBD dimer warheads and PBD-conjugated ADCs. We established a PBD-resistant cell line, 361-PBDr, by treating human breast cancer MDA-MB-361 cells with gradually increasing concentrations of SG3199, the PBD dimer released from the PBD drug-linker tesirine. 361-PBDr cells were over 20-fold less sensitive to SG3199 compared with parental cells and were cross-resistant to other PBD warhead and ADCs conjugated with PBDs. Proteomic profiling revealed that downregulation of Schlafen family member 11 (SLFN11), a putative DNA/RNA helicase, sensitizing cancer cells to DNA-damaging agents, was associated with PBD resistance. Confirmatory studies demonstrated that siRNA knockdown of SLFN11 in multiple tumor cell lines conferred reduced sensitivity to SG3199 and PBD-conjugated ADCs. Treatment with EPZ011989, an EZH2 inhibitor, derepressed SLFN11 expression in 361-PBDr and other SLFN11-deficient tumor cells, and increased sensitivity to PBD and PBD-conjugated ADCs, indicating that the suppression of SLFN11 expression is associated with histone methylation as reported. Moreover, we demonstrated that combining an ataxia telangiectasia and Rad3-related protein (ATR) inhibitor, AZD6738, with SG3199 or PBD-based ADCs led to synergistic cytotoxicity in either resistant 361-PBDr cells or cells that SLFN11 was knocked down via siRNA. Collectively, these data provide insights into potential development of resistance to PBDs and PBD-conjugated ADCs, and more importantly, inform strategy development to overcome such resistance.
Adverse postresuscitation myocardial effects elicited by buffer-induced alkalemia ameliorated by NHE-1 inhibition in a rat model of ventricular fibrillation (2016)

Major myocardial abnormalities occur during cardiac arrest and resuscitation including intracellular acidosis-partly caused by CO<sub>2</sub> accumulation-and activation of the Na<sup>+</sup>-H<sup>+</sup> exchanger isoform-1 (NHE-1). We hypothesized that a favorable interaction may result from NHE-1 inhibition during cardiac resuscitation followed by administration of a CO<sub>2</sub>-consuming buffer upon return of spontaneous circulation (ROSC). Ventricular fibrillation was electrically induced in 24 male rats and left untreated for 8 min followed by defibrillation after 8 min of cardiopulmonary resuscitation (CPR). Rats were randomized 1:1:1 to the NHE-1 inhibitor zoniporide or vehicle during CPR and disodium carbonate/sodium bicarbonate buffer or normal saline (30 ml/kg) after ROSC. Survival at 240 min declined from 100% with Zoniporide/Saline to 50% with Zoniporide/Buffer and 25% with Vehicle/Buffer (P = 0.004), explained by worsening postresuscitation myocardial dysfunction. Marked alkalemia occurred after buffer administration along with lactatemia that was maximal after Vehicle/Buffer, attenuated by Zoniporide/Buffer, and minimal with Zoniporide/Saline [13.3 ± 4.8 (SD), 9.2 ± 4.6, and 2.7 ± 1.0 mmol/l; P ≤ 0.001]. We attributed the intense postresuscitation lactatemia to enhanced glycolysis consequent to severe buffer-induced alkalemia transmitted intracellularly by an active NHE-1. We attributed the worsened postresuscitation myocardial dysfunction also to severe alkalemia intensifying Na<sup>+</sup> entry via NHE-1 with consequent Ca<sup>2+</sup> overload injuring mitochondria, evidenced by increased plasma cytochrome c Both buffer-induced effects were ameliorated by zoniporide. Accordingly, buffer-induced alkalemia after ROSC worsened myocardial function and survival, likely through enhancing NHE-1 activity. Zoniporide attenuated these effects and uncovered a complex postresuscitation acid-base physiology whereby blood pH drives NHE-1 activity and compromises mitochondrial function and integrity along with myocardial function and survival.
Investigation of liquid-vapor interactions in a constant area condensing ejector. (1967)

A detailed investigation of the liquid-vapor interactions occurring within a constant area steam-water condensing ejector is described. Axial static and radial impact pressure profiles were obtained. These data which correspond to a limited range of inlet vapor conditions and a wide range of inlet liquid velocities reveal the presence of three flow regimes based on inlet liquid velocity. Complete condensation caused by a condensation shock is shown to occur only within the High Inlet Liquid Velocity Regime. Evidence is given of the occurrence and importance of liquid jet breakup. It is shown that the presence of supersonic vapor flow is a necessary but not sufficient condition for the existence of the condensation shock. Two one-dimensional mixing section analyses are described. Digital computer solutions of the analyses are presented which show the effects of interfacial heat transfer and friction on the flow variables. It is shown that the coefficient of heat transfer from the interface to the liquid jet is of the order of 100 BTU/(sq. ft.) (sec) (degree F). This compares favorably with the results of other studies on the heat transfer rates to turbulent water jets with condensation. (Author)
Intermediate Filaments: A Chemically Heterogeneous, Developmentally Regulated Class of Proteins (1982)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Psychological Impact of Traumatic Events: (1989)

This paper reviews the increasing study of the psychological affects of traumatic events. The types of traumatic events which can impact individuals covered by employee assistance programs includes civilian disasters, criminal incidents, workplace accidents, and family abuse. Historical trends which outline current diagnostic and treatment issues are covered. Important symptom patterns are described, with review of the recovery issues following the immediate traumatic impact. Key intervention strategies for immediate crisis response and long-term follow-up are discussed with recommendation for comprehensive psychological trauma response. Employee assistance programs that assist individuals affected by traumatic events provide needed psychological safety net.
A Study of the Mechanism of Action Based on a Cultured Colonic Epithelial Cell Line (1989)

When applied to the basolateral (serosal) side of the T84 coIonic epithelial monolayer, taurodeoxycholate caused net Clsecretion in a dose-dependent manner with a threshold effect observed at 0.2 mM. In contrast, when applied to the apical (luminal) surface, concentrations oftaurodeoxycholate below 1 mM had little or no effect. Only when the concentration of taurodeoxycholate present on the apical side was 2 1 mM did apical addition results in an electrolyte transport effect. This apical effect on electrolyte transport was associated with an abrupt increase in the permeability of the monolayer. Cyclic AMP and cyclic GMP in the T84 monolayers were not increased by the bile salt, but in the presence of extracellular Ca2, free cytosolic Ca2 increased with a graded dose effect and time course that corresponded approximately to the changes in short circuit current (ISC). The results suggest that luminal bile salts at a relatively high concentration (2 1 mM) increase tight junction permeability. Once tight junction permeability increases, luminal bile salts could reach the basolateral membrane of the epithelial cells where they act to increase free cytosolic Ca2 from extracellular sources. The resulting increases in free cytosolic Ca2, rather than in cyclic nucleotides, appear to be involved in transcellular Cl- secretion.
Single layer iron powder core inductor model and its effect on boost PFC EMI noise (2003)

In this paper, the single layer iron powder inductor's general math model is studied and the model equations are derived. The simulations and measurements are processed to verify the model. The relations of the boost inductor impedance and DM noise spectrum are addressed in detail. Some suggestions on boost inductors are given to reduce the DM noise.
X-ray photoelectron spectroscopic study of organometallic nitrosyl compounds of chromium, molybdenum, and tungsten (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTX-ray photoelectron spectroscopic study of organometallic nitrosyl compounds of chromium, molybdenum, and tungstenH. W. Chen, W. L. Jolly, S. F. Xiang, and P. LegzdinsCite this: Inorg. Chem. 1981, 20, 6, 1779–1782Publication Date (Print):June 1, 1981Publication History Published online1 May 2002Published inissue 1 June 1981https://doi.org/10.1021/ic50220a035RIGHTS & PERMISSIONSArticle Views97Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (522 KB) Get e-Alerts Get e-Alerts
Optimized 25 V, 0.34 mΩ·cm/sup 2/ very-thin-RESURF (VTR), drain extended IGFETs in a compressed BiCMOS process (2002)

The competitive PC peripheral application market drives the goal to develop a compressed, low-cost BiCMOS power technology with state-of-the-art specific-on-resistance (R/sub sp/) at the 20 V node. The 20 V rated lateral power device is difficult to optimize because modern VLSI processes tend to physically limit surface BV to about 13-19 V in planar devices. Here the structure performance is advanced by optimizing a Very-Thin-RESURF (VTR) region (VTR Xj=0.3 /spl mu/m). This work presents a planar VTR drain extended IGFET with best case BV=25 V and R/sub sp/=0.34 m/spl Omega//spl middot/cm/sup 2/@V/sub gs/=10 V using a compressed BiCMOS VLSI, 1 /spl mu/m technology. Structure variation and thermal performance are characterized.
Strain‐specific immunomodulatory effects of Lactobacillus plantarum strains on birch‐pollen‐allergic subjects out of season (2010)

Subjects with seasonal allergy can be used to determine immunomodulatory responses outside the pollen season within a 4-week study period. L. plantarum CBS125632 decreased several immune markers related to allergy, and may have the potential to alleviate the severity of seasonal allergy symptoms.
An Optimal Control Benchmark : Transient Optimization of a Diesel-Electric Powertrain (2014)

An optimal control benchmark is presented and discussed. The benchmark is optimal transient control of a nonlinear four state three control model of a diesel-electric powertrain and constructed in such a manner that it is available in several versions to be of interest for developers of optimal control tools at different levels of development. This includes with and without time as a parameter as well as with and without time varying constraints.
Histopathological evaluation and immunohistochemical study of estrogen receptor α, HER-2 and Ki-67 in canine neoplastic mammary lesions. (2011)

The frequency of different types of mammary lesions and their relationship with histologic grade was investigated. One hundred and forty-six mammary lesions were classifi ed according to the World Health Organization (WHO) criteria. Selected lesions (51 malignant and 24 benign) were investigated to determine the immunohistochemical expression of estrogen receptor alpha (ERα), HER-2 and Ki-67 and their relationship with tumor factors. The most common breeds affected were cross breeds, poodles, cocker spaniels and German shepherds and the median age of tumor diagnosis was 10 years (range 4 15 years of age). Classifi cation of all canine mammary gland lesions revealed 110 (75.3%) malignant and 36 (24.7%) benign tumors. ERα was expressed by 9/24 (37.5%) benign and 9/51 (17.6%) malignant tumors. HER-2 protein was detected in 5/51 (9.8%) malignant tumors but none in the 24 benign tumors. The Ki-67 index was higher in malignant (mean 29.5) than benign (mean 9.1) tumors (P<0.001) and signifi cantly higher in anaplastic carcinomas than tubulopapillary carcinomas (P<0.05).
A computed method for noninvasive MRI assessment of pulmonary arterial hypertension (2004)

The present method enables the noninvasive assessment of mean pulmonary arterial pressure from magnetic resonance phase mapping by computing both physical and biophysical parameters. The physical parameters include the mean blood flow velocity over the cross-sectional area of the main pulmonary artery (MPA) at the systolic peak and the maximal systolic MPA cross-sectional area value, whereas the biophysical parameters are related to each patient, such as height, weight, and heart rate. These parameters have been measured in a series of 31 patients undergoing right-side heart catheterization, and the computed mean pulmonary arterial pressure value (Ppa Comp ) has been compared with the mean pressure value obtained from catheterization (Ppa Cat ) in each patient. A significant correlation was found that did not differ from the identity line Ppa Comp = Ppa Cat ( r = 0.92). The mean and maximal absolute differences between Ppa Comp and Ppa Cat were 5.4 and 11.9 mmHg, respectively. The method was also applied to compute the MPA systolic and diastolic pressures in the same patient series. We conclude that this computed method, which combines physical (whoever the patient) and biophysical parameters (related to each patient), improves the accuracy of MRI to noninvasively estimate pulmonary arterial pressures.
Comparative transcriptomics of pathogenic and non‐pathogenic Listeria species (2012)

Listeria monocytogenes is a human, food-borne pathogen. Genomic comparisons between L. monocytogenes and Listeria innocua, a closely related non-pathogenic species, were pivotal in the identification of protein-coding genes essential for virulence. However, no comprehensive comparison has focused on the non-coding genome. We used strand-specific cDNA sequencing to produce genome-wide transcription start site maps for both organisms, and developed a publicly available integrative browser to visualize and analyze both transcriptomes in different growth conditions and genetic backgrounds. Our data revealed conservation across most transcripts, but significant divergence between the species in a subset of non-coding RNAs. In L. monocytogenes, we identified 113 small RNAs (33 novel) and 70 antisense RNAs (53 novel), significantly increasing the repertoire of ncRNAs in this species. Remarkably, we identified a class of long antisense transcripts (lasRNAs) that overlap one gene while also serving as the 5' UTR of the adjacent divergent gene. Experimental evidence suggests that lasRNAs transcription inhibits expression of one operon while activating the expression of another. Such a lasRNA/operon structure, that we named 'excludon', might represent a novel form of regulation in bacteria.
ChemInform Abstract: Metalloclayzymes: An Efficient Oxidative Coupling of Thiols Catalyzed by Fe(III) Montmorillonite in Buffer. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Renewable energy for a green future: Electricity produced from efficient luminescent solar concentrators (2022)

This article proposes an overarching review of the technologies engaged in world wide energy demand which uses from non-renewable to renewable energies. The electricity generated from renewable systems is largely reducing CO2 emissions, one of the greenhouse gasses realesed from non-renewable energy sources that contribute to the global warming. The photovoltaic (PV) system is the technology with stronger potential to produce affordable electricity using solar energy. Therefore, we are focusing on the PVs regarding i) how they are assembled, ii) the energy losses from spectral mismatches, and iii) the improvements of their efficiencies by several ways, which include the connection of PVs with new techologies, e.g. the luminescent solar concentrator (LSC). Recent studies have reported advantages of applying LSCs connected to PVs’ cells, as well as some strategies for minimizing or eliminating self-absorption losses. Moreover, some efficiency meters evaluate LSCs’ operation. Guided by mainly recent researches, this article approaches flexibility, low cost, easy fabrication, and great efficiency of LSCs, the main goal for the PVs’ productions upon power electricity demand.
Structural Characterization of HC-Pro, a Plant Virus Multifunctional Protein (2003)

The helper component proteinase (HC-Pro) is a key protein encoded by plant viruses of the genus Potyvirus. HC-Pro is involved in different steps of the viral cycle, aphid transmission, replication, and virus cell-to-cell and systemic movement and is a suppressor of post-transcriptional gene silencing. Structural knowledge of HC-Pro is required to better understand its multiple functions. To this aim, we purified His-tagged wild-type HC-Pro and a N-terminal deletion mutant (DeltaHC-Pro) from plants infected with recombinant potyviruses. Biochemical analysis of the recombinant proteins confirmed that HC-Pro is a dimer in solution, that the N terminus is not essential for self-interaction, and that a large C-terminal domain is highly resistant to proteolysis. Two-dimensional crystals of the recombinant proteins were successfully grown on Ni2+-chelating lipid monolayers. Comparison of projection maps of negatively stained crystals revealed that HC-Pro is composed of two domains separated by a flexible constriction. Cryo-electron crystallography of DeltaHC-Pro allowed us to calculate a projection map at 9-A resolution. Our data from electron microscopy, biochemical analysis, and secondary structure predictions lead us to suggest a model for structure/function relationships in the HC-Pro protein.
Supplementary Data. from <i>HER2</i> Status in Advanced or Metastatic Gastric, Esophageal, or Gastroesophageal Adenocarcinoma for Entry to the TRIO-013/LOGiC Trial of Lapatinib (2023)

&lt;p&gt;Supplementary Data: List of TRIO investigators, Supplementary Materials and Methods, Supplementary Results, Supplementary Discussion, Supplementary References, Supplementary Tables, and Supplementary Figures.&lt;/p&gt;
Ultrasensitive gas detection using diode lasers and resonant photoacoustic spectroscopy (2002)

A novel trace-gas sensor system has been developed based on resonant photoacoustics, wavelength modulation spectroscopy, near-infrared diode lasers and optical fiber amplifiers that can achieve parts-per-billion sensitivity with a ten centimeter long sample cell and standard commercially-available optical components. An optical fiber amplifier with 500 mW output power is used to increase the photoacoustic signal by a factor of 25, and wavelength modulation spectroscopy is used to minimize the interfering background signal from window absorption in the sample cell, thereby improving the overall detection limit. This sensor is demonstrated with a diode laser operating near 1532 nm for detection of ammonia that achieves an ultimate sensitivity of less than 6 parts-per-billion. The minimum detectable fractional optical density, <i>&#945;<sub>min</sub>l</i>, is 1.8x10<sup>-8</sup>, the minimum detectable absorption coefficient, <i>&#945;<sub>min</sub></i>, is 9.5x10<sup>-10</sup> cm<sup>-1</sup>, and the minimum detectable absorption coefficient normalized by power and bandwidth is 1.5x10<sup>-9</sup> Wcm<sup>-1</sup>/&sqrt; Hz. These measurements represent the first use of fiber amplifiers to enhance photoacoustic spectroscopy, and this technique is applicable to all other species that fall within the gain curves of optical fiber amplifiers.
Knowledge-based retrieval of information as a process of evidential reasoning (1990)

Knowledge-based retrieval of information has been proved to enhance the performance of conventional retrieval systems. Systems like RUBRIC (RUle-Based Retrieval of Information by Computer) and KADR (Knowledge-Assisted Document Retrieval) have successfully incorporated the techniques developed in the research of knowledge-based systems to solve the retrieval problem.
Current knowledge-based retrieval systems, however, share several common problems. They are designed either for special users or their reasoning mechanism is not general enough. When developed for specific users, they reflect user preference instead of expert knowledge; when the reasoning mechanism is not sufficiently general, an inadequate model could lead to counterexamples.
The focus of this research is to design a theoretically sound knowledge-based retrieval system for general users. In this system, expert knowledge serves as a basis for automatic query formulation and query evaluation; furthermore, the entire retrieval of information is considered to be a process of evidential reasoning. The foundation of the reasoning methodology is Dempster-Shafer theory and its extension. The new approach subsumes the conventional Boolean system and has advantages over the other retrieval systems.
REDER (REtrieval of Documents based on Evidential Reasoning) is a prototype of such a system and demonstrates a complete process of knowledge-based retrieval of information. The results of a retrieval experiment verifies the performance of this system.
The Effects of Various LED Light Wavelengths to the Physiological and Morphological Parameters of Stevia (<i>Stevia rebaudiana</i>) Bertoni (2016)

In this study, it was investigated the growth of stevia (Stevia rebaudiana Bertoni) under various wavelengths of LED lamp (Light Emitting Diodes), which can emit daylight (cool white; 400–700 nm), red (620–630 nm) and blue (465–485 nm) wavelengths of the light in the electromagnetic spectrum. In all applications, quantity of PAR (photosynthetically active radiation) was adjusted as 150 µmol.m-2 s-1. Study had maintained in plant breeding cabin 16 hours light and 8 hours dark environment. Results demonstrated that while the highest plant height was determined in the “30% blue light+ 70% red light” application, the highest stem length was found in the “50% blue light + 50% red light” application. In addition, the number of the stems reached the highest value in the “70% blue light + 30% red light” application. Consequently, a correlation was observed between negative “a” value and the amount of chlorophyll. Because of the hereby obtained results, comparing to other applications, the “50% blue light + 50% red light” was found as the best light application to obtain optimum yield values of stevia.