gebhart/patrepeval huggingface dataset:
Interaction of nuclear proteins with muscle-specific regulatory sequences of the human cardiac alpha-actin promoter. (1988)

The human cardiac alpha-actin promoter is involved in the muscle-specific transcriptional regulation of the gene. In this study, we utilized gel mobility shift, methylation interference, and DNase I protection assays to examine protein factor interaction with the promoter in vitro. All assays demonstrated specific interaction of nuclear factors with a region of the promoter encompassed by nucleotides -93 to -113 base pairs from the transcriptional start site. This region contains a CC(A + T-rich)6GG element, termed a CArG box, which has previously been implicated in the muscle-specific transcriptional regulation of the gene by functional assays. Although the gene is only expressed in muscle cells, identical binding activity was present in nuclear extracts of all cell types examined, including those of muscle (C2, L8, and L6 cells) and nonmuscle (HeLa, NIH 3T3, HuT12, and L cells) origin. Furthermore, methylation interference assays showed that identical nucleotides interacted with factors isolated from C2 and HeLa cells. Competition studies showed that the CArG-binding factor, designated as CBF, also interacts with the c-fos serum responsive element, which contains a CArG element, but not with the simian virus 40 enhancer and early promoter. Thus, a region of the human cardiac alpha-actin promoter known to be functionally involved in muscle-specific regulation of the gene appears to interact in vitro, and in an identical manner, with a factor(s) which is neither muscle nor gene specific, suggesting a more complex mode of regulation than previously envisioned.
Radionuclide Urodynamic Studies in Patients with Bladder Outlet Obstruction (1990)

Various urodynamic studies have been used in patients with bladder outlet obstruction in order to evaluate the degree of obstruction, the results of therapy and postprostatectomy conditions. Radionuclide urodynamic study was performed in 27 patients with bladder outlet obstruction and 30 normal controls. The parameters evaluated were voiding time, 50% voiding time, average flow rate, peak flow rate, corrected peak flow rate, ejection fraction of the bladder and residual urine. Voiding time, 50% voiding time and residual urine of patients were significantly larger than controls and average flow rate, Peak flow rate, peak corrected flow rate and ejection fraction were significantly lower in patients. This method was noninvasive procedure for determining of voiding parameters and it avoids the extraexamination needs to determine the residual urine.
CXCR7 Functions as a Scavenger for CXCL12 and CXCL11 (2010)

The finding that CXCR7 specifically scavenges CXCL12 suggests a critical function of the receptor in modulating the activity of the ubiquitously expressed CXCR4 in development and tumor formation. Scavenger activity of CXCR7 might also be important for the fine tuning of the mobility of hematopoietic cells in the bone marrow and lymphoid organs.
A Dual Role for the S-Locus Receptor Kinase in Self-Incompatibility and Pistil Development Revealed by an Arabidopsis rdr6 Mutation (2009)

The coordinate evolution of self-incompatibility (SI) and stigma-anther separation, two mechanisms that promote cross-pollination in plants, has been a long-standing puzzle in evolution and development. Using a transgenic self-incompatible Arabidopsis thaliana model, we performed screens for mutants exhibiting a modified SI response. A mutation in the RNA-dependent RNA polymerase RDR6, which functions in trans-acting short interfering RNA (ta-siRNA) production, was found that simultaneously enhances SI and causes stigma exsertion, without associated increases in SRK transcript levels. While rdr6 mutants had been previously shown to exhibit stochastic stigma exsertion, our results demonstrate that the S-locus receptor kinase (SRK) gene further enhances pistil elongation and stigma exsertion in this mutant background, a process that requires SRK catalytic activity and correlates with SRK transcript levels. These results suggest that positive regulators or effectors of SI and pistil development are regulated by ta-siRNA(s). By establishing complex connections between SI and stigma exsertion through the sharing of a ta-siRNA-mediated regulatory pathway and the dual role of SRK in SI and pistil development, our study provides a molecular explanation for the coordinate evolution of these processes.
Severe Hypomyelination of the Murine CNS in the Absence of Myelin-Associated Glycoprotein and Fyn Tyrosine Kinase (2000)

The analysis of mice deficient in the myelin-associated glycoprotein (MAG) or Fyn, a nonreceptor-type tyrosine kinase proposed to act as a signaling molecule downstream of MAG, has revealed that both molecules are involved in the initiation of myelination. To obtain more insights into the role of the MAG-Fyn signaling pathway during initiation of myelination and formation of morphologically intact myelin sheaths, we have analyzed optic nerves of MAG-, Fyn- and MAG/Fyn-deficient mice. We observed a slight hypomyelination in optic nerves of MAG mutants that was significantly increased in Fyn mutants and massive in MAG/Fyn double mutants. The severe morphological phenotype of MAG/Fyn mutants, accompanied by behavioral deficits, substantiates the importance of both molecules for the initiation of myelination. The different severity of the phenotype of different genotypes indicates that the MAG-Fyn signaling pathway is complex and suggests the presence of compensatory mechanisms in the single mutants. However, data are also compatible with the possibility that MAG and Fyn act independently to initiate myelination. Hypomyelination of optic nerves was not related to a loss of oligodendrocytes, indicating that the phenotype results from impaired interactions between oligodendrocyte processes and axons and/or impaired morphological maturation of oligodendrocytes. Finally, we demonstrate that Fyn, unlike MAG, is not involved in the formation of ultrastructurally intact myelin sheaths.
Mechanism of the postreceptor defect in insulin action in human obesity. Decrease in glucose transport system activity. (1981)

We have studied insulin-stimulated 3-O-methyl glucose transport by isolated adipocytes prepared from 10 normal and 11 obese individuals. The results demonstrated that the insulin-glucose transport dose-response curves were shifted to the right in cells from the obese patients, and that the magnitude of this rightward shift was significantly correlated to the reduction in adipocyte insulin receptors in individual subjects (r = 0.48, P less than 0.01). In three obese patients a rightward shift in the dose-response curve could be demonstrated and there was no decrease in maximal insulin effect. This corresponded to in vivo glucose clamp results showing only a rightward shift in the insulin dose-response curve for overall glucose disposal in these three subjects (1980. J. Clin. Invest. 65: 1272-1284). In the remaining eight obese patients, the in vitro glucose transport studies showed not only a rightward shift in the dose-response curves but also a marked decrease in basal and maximally insulin-stimulated rates of transport, indicating a postreceptor defect in insulin action. Again, this was consistent with the in vivo glucose clamp studies demonstrating a marked postreceptor defect in these individuals. In conclusion, these results indicate that the mechanism of the postreceptor defect in insulin action, which exists in many obese patients, is related to a decrease in the activity of the glucose transport effector system.
A multitranscript blood neuroendocrine tumor molecular signature to identify treatment efficacy and disease progress. (2013)

4137 Background: Gastroenteropancreatic (GEP) neuroendocrine neoplasms (NENs) or NETs (also called “carcinoids”) are problematic since they present with advanced disease and have limited treatment options. Imaging has proved to be insensitive in identifying treatment effect. Similarly, the currently used biomarker, a neurosectory peptide, Chromogranin A (CgA) is of limited value as a predictor of treatment efficacy. We have developed a multi-transcript (n=51 genes) molecular signature for PCR-based blood analysis which exhibits a high sensitivity (85-98%), specificity (93-97%), positive predictive value (95-96%) and negative predictive value (87-98%) for the identification of GEP-NENs. This test significantly outperforms (p&lt;0005) plasma CgA (measured by ELISA). Methods: The candidate gene signature was examined by a 2-step PCR method in a training set of 130 blood samples (GEP-NENs: n=63, treatment-naïve (n=28)) and validated in an independent set ([n=182, GEP-NENs: n=133, including complete remission (n=4), clinically stable disease (n=82) and non-responders/clinically progressive disease (n=47). Results: The PCR score was significantly elevated (p&lt;0.0001) in the treatment-naïve group compared to treated GEP-NENs, while levels were significantly reduced (p&lt;0.004) in 11 matched samples (pre- and post-treatment – surgical removal (n=10), RFA (n=1)). In the independent set, the scores for complete remission were not different to controls (0.5±0.25 vs. 0.4±0.16), while scores for stable disease (0.63±0.12) were significantly lower than for non-responders (5.85±0.34, p&lt;0.002). Stable disease could be differentiated from clinically progressive disease since the latter exhibited a high score (85% vs. 10%, p&lt;0.0001). The performance metrics for differentiation were: sensitivity (91%), specificity (91%), PPV (86%), NPV (95%). Conclusions: This study demonstrated that the multi-transcript molecular signature is both sensitive and specific for the detection of neuroendocrine tumor disease and is capable of differentiating clinically stable from progressive disease. It therefore has potential utility as a measure for monitoring treatment efficacy for GEP-NENs.
Pumping characteristics of the St707 nonevaporable getter (Zr 70 V 24.6-Fe 5.4 wt %) (1996)

The room temperature pumping speeds of the St707 nonevaporable getter (NEG) have been measured both for individual gases and for gas mixtures as a function of the quantities of gas pumped. The interesting feature of this NEG consists in its moderately low activation temperature. Therefore particular attention has been devoted to defining the optimum temperature and duration of the activation process to obtain the highest possible pumping speed in a given practical situation. It has been found that heating at 400 °C for about 1 h, or at 350 °C for one day, results in pumping speeds of about 1000 ℓ s−1 m−1 for H2, 2000 ℓ s−1 m−1 for CO, and 450 ℓ s−1 m−1 for N2, values very close to those obtained after activation at the higher temperature of 740 °C. The St707 NEG is therefore particularly suitable for passive activation during bakeout of stainless steel vacuum systems, avoiding the need for electrical insulation and feedthroughs which are mandatory when activation is carried out by resistive heating.
Chemiluminescence from concerted peroxide decomposition reactions (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemiluminescence from concerted peroxide decomposition reactionsMichael M. RauhutCite this: Acc. Chem. Res. 1969, 2, 3, 80–87Publication Date (Print):March 1, 1969Publication History Published online1 May 2002Published inissue 1 March 1969https://pubs.acs.org/doi/10.1021/ar50015a003https://doi.org/10.1021/ar50015a003research-articleACS PublicationsRequest reuse permissionsArticle Views1725Altmetric-Citations235LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Self-starting mode-locked femtosecond forsterite laser with a semiconductor saturable-absorber mirror (1997)

We report on a self-starting mode-locked femtosecond Cr:forsterite laser pumped by a diode-pumped Nd:YVO(4) laser. The mode locking is initiated by a semiconductor saturable-absorber mirror (SESAM). We also present the measured group delay of the forsterite crystal and the SESAM.
P-24: a human leukemia-associated and lymphohemopoietic progenitor cell surface structure identified with monoclonal antibody. (1981)

This study was directed at surface structures that are found on human lymphohemopoietic progenitor cells in normal and leukemic bone marrow. A monoclonal antibody was produced against an acute lymphoblastic leukemia (ALL) cell line of the pre-B phenotype; this antibody (BA-2) was used to demonstrate a cell surface polypeptide of approximately 24,000 mol wt that migrates similarly in both reduced and nonreduced form. This polypeptide, p24/BA-2, was shown by immune precipitation and gel electrophoresis and cell distribution studies to be different from HLA-DR and gp 100/cALLa. p24/BA-2 was present on the surface of 77% (54/70) of cases of non-T, non-B ALL; BA-2 staining was less bright or nondetectable in surface Ig+ (SIg+) chronic lymphocytic leukemia (CLL) and T ALL and nondetectable on peripheral T and B lymphocytes. Approximately 3% of bone marrow mononuclear cells were p24/BA-2+, and these cells were E rosette-, surface (SIg-), and nonphagocytic. Marrow TdT+ progenitor cells were frequently p 24/BA-2+. Results suggest that p24/BA-2 represents a surface structure present on lymphohemopoietic bone marrow progenitor cells and that most common types of ALL bear the p25/BA-2 structure.
Bacteriophage T4 Host Range is Expanded by Duplications of a Small Domain of the Tail Fiber Adhesin (1996)

The adsorption specificity of T4 is determined by the tip of the gene 37 tail fibers which bind to receptors on the bacterial surface. T4 infects only Escherichia coli and closely related Shigella species, but rare host range mutants can be isolated that infect Yersinia pseudotuberculosis I, an evolutionally distant bacterium. Some of these mutations result in amino acid residue substitutions in the C-terminal portion of gene 37, but others involve unequal exchanges between a series of sequence motifs (His boxes) in the same region. The duplication or mutational alteration of this segment apparently suffices for phage adsorption to a Yersinia receptor. It is suggested that recombination between the His box sequences can generate diversity in phage host range by shuffling receptor recognition domains.
Structure of PatF fromProchloron didemni (2013)

Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.
Nanoformulations of Drugs Based on Biodegradable Lactide Copolymers with Various Molecular Structures and Architectures (2021)

Modern pharmaceutics are actively developing towards the design of targeted drugs. The development of selectively acting formulations requires the creation of smart delivery systems based on carriers that would first find the target cells and enter them and then release the active substance locally. Nanoparticles of biocompatible and biodegradable polymers can be effectively used as such carriers. Flexible regulation of the molecular structure and architecture of polymers, as well as the modification of nanoparticles with vector molecules, allows one to construct carrier particles for the development of nanoformulations for active agents of various nature. This review presents the main approaches to the design of nanoformulations for targeted delivery, describes the methods for the preparation and study of nanoparticles based on hydrophobic and amphiphilic biodegradable lactide polymers, and discusses the effect of the molecular structure and preparation conditions on the characteristics of nanoparticles in detail. Some results of research in this area of the Kurchatov complex of NBIСS nature-like technologies are also presented.
Comparison of Modal Equalizer Design Methods (2003)

Modal equalization of low-frequency room modes has recently been proposed as a method to improve sound reproduction in spaces where modal decay time is too long. Modal equalization is achieved by signal processing reducing the pole radii of problematic modes in the overall transfer function. In this paper, we compare the performance of two proposed methods for designing modal equalizers. Comparison includes a preliminary subjective listening test indicating a possible marginal improvement by modal equalization over conventional magnitude equalization.
Femoral Intercondylar Notch Stenosis and Correlation to Anterior Cruciate Ligament Injuries (1994)

To evaluate the possible relationship between femoral intercondylar notch stenosis and anterior cruciate liga ment injuries in pivoting and cutting sports, a 2-year prospective study was performed on intercollegiate ath letes at a Division I university. Daily practice times and athlete participation in practices and games were re corded for each sport during the 2-year period. Bilateral intercondylar notch view radiographs were taken of all athletes enrolled in the study. The notch width index, a ratio that measures the width of the anterior outlet of the intercondylar notch divided by the total condylar width at the level of the popliteal groove, was measured for each knee. A total of 213 athletes, representing 415 anterior cruciate ligament-intact knees, were enrolled in the study. There were 7 anterior cruciate ligament tears. Statistical analysis demonstrated a correlation between femoral intercondylar notch stenosis and anterior cru ciate ligament injuries. No statistical difference was found between the sex of the athlete and notch width indices or rate of anterior cruciate ligament tears. Ath letes with intercondylar notch stenosis appear to be at increased risk for noncontact anterior cruciate ligament injuries.
Interleukin-11 and its receptor. (1992)

Interleukin (IL)-11 is a bone marrow fibroblast derived cytokine with a wide spectrum of activities in different biological systems. It has been shown that IL-11 supports the growth of certain types of plasmacytoma and hybridoma cells, enhances antigen-specific antibody responses, synergizes with IL-3 in supporting megakaryocyte colony formation, acts synergistically with IL-3 in shortening the G0 period of early progenitors, induces the synthesis of acute phase proteins, and inhibits lipoprotein lipase activity and adipocyte differentiation. The human IL-11 gene, which is localized at 19q13.3-13.4, consists of five exons and four introns. Initial biochemical characterization has identified a 151 kDa protein as the potential IL-11 binding subunit of the receptor complex. Because of the overlapping biological activities between IL-6 and IL-11, we compared the signal transduction pathways mediated by IL-6 or IL-11 in cell lines responsive to both cytokines. Results from protein tyrosine phosphorylation and immediate response gene expression suggest that there are convergent and divergent points along the signal transduction pathways utilized by IL-6 or IL-11. The IL-6 signal transducer, gp130, appears to be involved in the IL-11 mediated signaling. Other cytokines such as leukemia inhibitory factor, oncostatin M and ciliary neurotrophic factor have also been shown to utilize gp130 as a signal transducer. The significance of growth factor sharing common biological activities and signaling pathways will be discussed.
On treating the insane in sane places (1977)

Five major issues raised by Rosenhan (1973) about psychiatric hospitals are addressed. For each issue, a resolution is presented and the method of implementing the resolution in the Southwest Denver inpatient hospital alternative system is described. In this system, clinical staff offices have been eliminated; the salience of psychiatric diagnosis has been diminished; it is assumed that, sane or insane, no client needs inpatient admission until proven otherwise. When admission is indicated, a comprehensive range of alternative settings is available, including a network of private homes which contract with the Center to provide room, board, and informal care for clients admitted. The results of a longitudinal research study designed to assess the viability of the private home as an alternative to hospitalization are presented, particularly as they bear on the negative effects of hospitalization as described by Rosenhan.
A Rapid and Simple Cleanup Procedure for Metathesis Reactions (2007)

[structure: see text]. A new method for easy removal of ruthenium from metathesis reactions by using a polar isocyanide is reported. This protocol removed most ruthenium byproducts from a variety of synthetically useful metatheses. Moreover, the isocyanide-promoted carbene insertion results in rapid destruction of carbene reactivity, demonstrated in the commonly used first- and second-generation Grubbs' carbenes.
Refrescos Ácidos: Dissolução do Esmalte (2010)

Introduction: erosion is the superficial loss of enamel caused by chemical process do not involve bacterias but extrinsic factors as the high consumption of low pH drinks.  Aim: evaluate the enamel superficial roughness of the bovine teeth after the contact of acid solutions. Material and methods: lemonade (group 1), artificial juice in powder with lemon flavor (group 2), and a light soft drink made of cola and lemon (group 3), evaluate the enamel superficial roughness of the bovine after action of solutions. Results: the roughness average was: GI (0,0120), GII (0,3215), GIII (0,0275). Conclusion: the beverage’s effects and the time were statistically significant, GII showed the biggest superficial roughness when compared with GI and GIII.
On prediction of wind-borne plumes with simple models of turbulent transport (2006)

The dispersion of pollutants from the ground by turbulent winds is difficult to model in general.However, for flat homogeneous terrain and steady wind conditions, if the wind profile is modeled with a power-law dependence on height, the advection-dispersion equation has an exact solution.In this paper the analytical solution is compared to a numerical simulation of the coupled air-ground system for a leaking underground gas storage, with a power-law velocity profile that was fit to the logarithmic velocity profile used in the simulation.The two methods produced similar results far from the boundaries, but the boundary conditions had a strong effect; the simulation imposed boundary conditions at the edge of a finite domain while the analytic solution imposes them at infinity.The reverse seepage from air to ground was shown in the simulation to be very small, and the sharp contrast between time scales suggests that air and ground can be modeled separately, with gas emissions from the ground model used as inputs to the air model.