gebhart/patrepeval huggingface dataset:
Design of a Ti-based microsensor for in vivo pressure monitoring (2019)

In this paper, the design of a novel bulk-titanium-based capacitive sensor for in vivo pressure monitoring was modeled analytically and by finite element method (FEM). In vivo pressure monitoring is one of the most relevant parameters to measure, because it can be correlated to patient health (blood, bladder, eyes, skull, abdomen). However, existing microsensors based on non-biocompatible materials such as silicon suffer from complex, bulky and costly biocompatible packaging, which also affects the sensors sensitivity. Progress on Ti MEMS technologies open promising ways to overcome these issues, as Ti material is already largely used in the industry of medical implants for its excellent mechanical properties, resistance to corrosion and biocompatibility. Recent results on dry etching showed the possibility to realize bulk-titanium-based microsystems. Here, we propose a capacitive pressure sensor combining two structures: a micropillar and a Ti membrane.
Estimation of changing gross tumor volume from longitudinal CTs during radiation therapy delivery based on a texture analysis with classifier algorithms: a proof-of-concept study (2019)

It is feasible to estimate the general region of the GTV of the day from the daily CT acquired during RT, based on CT textures, using a trained voxel classifier algorithm. The obtained GTV may be used as a starting point for an accurate GTV delineation in online adaptive replanning. Further study with larger patient datasets is required to improve the robustness of the algorithms.
Data from Overexpression of the Cellular DEK Protein Promotes Epithelial Transformation <i>In vitro</i> and <i>In vivo</i> (2023)

&lt;div&gt;Abstract&lt;p&gt;High levels of expression of the human &lt;i&gt;DEK&lt;/i&gt; gene have been correlated with numerous human malignancies. Intracellular DEK functions have been described &lt;i&gt;in vitro&lt;/i&gt; and include DNA supercoiling, DNA replication, RNA splicing, and transcription. We have shown that DEK also suppresses cellular senescence, apoptosis, and differentiation, thus promoting cell growth and survival in monolayer and organotypic epithelial raft models. Such functions are likely to contribute to cancer, but direct evidence to implicate DEK as an oncogene has remained elusive. Here, we show that in line with an early role in tumorigenesis, murine papilloma formation in a classical chemical carcinogenesis model was reduced in DEK knockout mice. Additionally, human papillomavirus E6/E7, hRas, and DEK cooperated in the transformation of keratinocytes in soft agar and xenograft establishment, thus also implicating DEK in tumor promotion at later stages. Finally, adenoviral DEK depletion via short hairpin RNA expression resulted in cell death in human tumor cells &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;, but did not significantly affect differentiated epithelial cells. Taken together, our data uncover oncogenic DEK activities as postulated from its frequent up-regulation in human malignancies, and suggest that the targeted suppression of DEK may become a strategic approach to the treatment of cancer. [Cancer Res 2009;69(5):1792–9]&lt;/p&gt;&lt;/div&gt;
New Polarization-detecting Optical Sensor for Positioning to Matrix Cross-connection Hole on a Printed Board. (1991)

To realize precision positioning systems, a new polarization-detecting optical sensor has been developed. The sensor can detect 3-dimensional translation error (x,y,z) and 2-dimensional rotation error (4),1lJ) between the sensor and the printed board with guide patterns. The sensor is constructed by using two polarized lights
High-Quality Genome-Scale Models From Error-Prone, Long-Read Assemblies (2020)

Advances in nanopore-based sequencing techniques have enabled rapid characterization of genomes and transcriptomes. An emerging application of this sequencing technology is point-of-care characterization of pathogenic bacteria. However, genome assessments alone are unable to provide a complete understanding of the pathogenic phenotype. Genome-scale metabolic reconstruction and analysis is a bottom-up Systems Biology technique that has elucidated the phenotypic nuances of antimicrobial resistant (AMR) bacteria and other human pathogens. Combining these genome-scale models (GEMs) with point-of-care nanopore sequencing is a promising strategy for combating the emerging health challenge of AMR pathogens. However, the sequencing errors inherent to the nanopore technique may negatively affect the quality, and therefore the utility, of GEMs reconstructed from nanopore assemblies. Here we describe and validate a workflow for rapid construction of GEMs from nanopore (MinION) derived assemblies. Benchmarking the pipeline against a high-quality reference GEM of <i>Escherichia coli K</i>-12 resulted in nanopore-derived models that were >99% complete even at sequencing depths of less than 10× coverage. Applying the pipeline to clinical isolates of pathogenic bacteria resulted in strain-specific GEMs that identified canonical AMR genome content and enabled simulations of strain-specific microbial growth. Additionally, we show that treating the sequencing run as a mock metagenome did not degrade the quality of models derived from metagenome assemblies. Taken together, this study demonstrates that combining nanopore sequencing with GEM construction pipelines enables rapid, <i>in situ</i> characterization of microbial metabolism.
Speech acoustic-cue discrimination abilities of normally developing and language-impaired children (1981)

The ability of 35 language impaired (LI) and 38 normally developing children between the ages of 5 and 8 1/2 yr to discriminate between speech stimuli, using an operantly conditioned, nonverbal motor response, was investigated. Six syllable contrasts ([ba] versus [da], [da] versus [ta], [epsilon] versus [ae], [dab] versus [daeb], [sa] versus [sta] and [sa] versus [integral a]) were selected to investigate discrimination of various temporal and spectral cues. Results demonstrated that the LI group made most errors discriminating syllables which were differentiated by consonants and fewest errors on those differentiated by vowels. The normal group made most errors discriminating between syllables including fricatives and brief vowels and fewest errors on stimuli differentiated by stop consonants or vowels in context. The LI group was significantly poorer than the normal in discriminating all syllables that incorporated brief temporal cues followed rapidly in succession by other acoustic cues. They also were impaired in discriminating [sa] versus [integral a]. They were unimpaired discriminating stimuli differentiated by vowels. It is suggested that the particular constraints demonstrated by LI children in their nonverbal and speech processing abilities may be attributed to abnormalities in mechanisms involved in auditory masking.
Obstacle Prediction-based Dynamic Path Planning for a Mobile Robot (2012)

A path-planning method based on a combination of the static global and the dynamic local pathplanning methods is proposed for robot path planning under a complex environment. There are known static obstacles and unknown dynamic obstacles in any complex environment. The local-path planner dynamically generates a local path using obstacle-motion prediction and a rolling window for dynamic path planning to partially adjust the global path. Simulation results show that the mobile robot achieves both overall and local obstacle-avoidance during motion, in association with an optimum path, which verifies the feasibility and effectiveness of the method.
Multicenter evaluation of a homogeneous assay for HDL-cholesterol without sample pretreatment. (1997)

We evaluated a new homogeneous assay for the measurement of HDL-cholesterol (HDL-C) in six European laboratories. The assay includes two reagents and is applicable to most autoanalyzers, which allows full automation. The total CVs of the new method ranged between 1.3% and 6.7%. Thereby determined HDL-C values were in good agreement with those obtained by precipitation with phosphotungstic acid/MgCl2 or by a combination of ultracentrifugation and precipitation (0.956 < r < 0.994). The assay was linear up to at least 1500 mg/L HDL-C. Hemoglobin did not interfere, whereas icteric samples with bilirubin > 100 mg/L showed discrepancies between the homogeneous and the precipitation assay. Lipemia up to total triglyceride concentrations of 8000 mg/L did not interfere with the homogeneous HDL-C assay. The homogeneous HDL-C assay was easy to handle and produced similar results in all laboratories participating in this study. This method will significantly facilitate the screening of individuals at increased risk for cardiovascular disease.
Preventable fractions of colon and breast cancers by increasing physical activity in Brazil: perspectives from plausible counterfactual scenarios (2018)

High levels of physical activity are required to achieve a sizable impact on breast and colon cancer prevention in Brazil.
La convention ACP-CEE de Lomé (28 février 1975) (2017)

Le 28 fevrier 1975, quarante-six Etats d'Afrique, des Caraibes et du Pacifique (ACP) et les neuf Etats membres de la Communaute economique europeenne (CEE) signent a Lome (Togo) une convention de cooperation.
Designing seeds for similarity search in genomic DNA (2003)

Large-scale comparison of genomic DNA is of fundamental importance in annotating functional elements of genomes. To perform large comparisons e.ciently, BLAST [3, 2] and other widely used tools use seeded alignment, which compares only sequences that can be shown to share a common pattern or "seed" of matching bases. The literature suggests that the choice of seed substantially affects the sensitivity of seeded alignment, but designing and evaluating seeds is computationally challenging. This work addresses problems arising in seed design. We give the fastest known algorithm for evaluating the sensitivity of a seed in a Markov model of ungapped alignments, as well as theoretical results on which seeds are good choices. We also describe Mandala, a software tool for seed design, and show that it can be used to improve the sensitivity of alignment in practice.
Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation. (2016)

The probability of detecting influenza A virus (IAV) in oral fluid (OF) specimens was calculated for each of 13 assays based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) and 7 assays based on virus isolation (VI). The OF specimens were inoculated with H1N1 or H3N2 IAV and serially diluted 10-fold (10(-1) to 10(-8)). Eight participating laboratories received 180 randomized OF samples (10 replicates × 8 dilutions × 2 IAV subtypes plus 20 IAV-negative samples) and performed the rRT-PCR and VI procedure(s) of their choice. Analysis of the results with a mixed-effect logistic-regression model identified dilution and assay as variables significant (P < 0.0001) for IAV detection in OF by rRT-PCR or VI. Virus subtype was not significant for IAV detection by either rRT-PCR (P = 0.457) or VI (P = 0.101). For rRT-PCR the cycle threshold (Ct) values increased consistently with dilution but varied widely. Therefore, it was not possible to predict VI success on the basis of Ct values. The success of VI was inversely related to the dilution of the sample; the assay was generally unsuccessful at lower virus concentrations. Successful swine health monitoring and disease surveillance require assays with consistent performance, but significant differences in reproducibility were observed among the assays evaluated.
Thermodynamic properties and phase equilibria of the potassium-indium system by electromotive force measurements (1991)

The potassium-indium system has been studied using galvanic cells with potassium beta alumina as the electrolyte. The thermodynamic properties of the liquid solution have been determined between 0 and 52 at.% potassium. There is some evidence for a very weak liquid compound near 35 at.% K, but not at 50 at.% K. Changes are proposed for the potassium-indium phase diagram, including the liquidus temperature and the existence of new compounds.
A Novel Cas Family Member, HEPL, Regulates FAK and Cell Spreading (2008)

For over a decade, p130Cas/BCAR1, HEF1/NEDD9/Cas-L, and Efs/Sin have defined the Cas (Crk-associated substrate) scaffolding protein family. Cas proteins mediate integrin-dependent signals at focal adhesions, regulating cell invasion and survival; at least one family member, HEF1, regulates mitosis. We here report a previously undescribed novel branch of the Cas protein family, designated HEPL (for HEF1-Efs-p130Cas-like). The HEPL branch is evolutionarily conserved through jawed vertebrates, and HEPL is found in some species lacking other members of the Cas family. The human HEPL mRNA and protein are selectively expressed in specific primary tissues and cancer cell lines, and HEPL maintains Cas family function in localization to focal adhesions, as well as regulation of FAK activity, focal adhesion integrity, and cell spreading. It has recently been demonstrated that upregulation of HEF1 expression marks and induces metastasis, whereas high endogenous levels of p130Cas are associated with poor prognosis in breast cancer, emphasizing the clinical relevance of Cas proteins. Better understanding of the complete protein family should help inform prediction of cancer incidence and prognosis.
ChemInform Abstract: Aglycon Mimicking: Glycoside Bond Cleavage Transition State Mimics Based on Hydroxypyrrolidine Inhibitors. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Parasitic effects and polarization diversity of archimedean spiral antenna (1983)

Abstract For the purpose of controlling the polarization of an Archimedean spiral antenna (SA), a parasitic spiral element is added on the same plane of a two‐wire SA. the radiation characteristics have been studied numerically and experimentally. First, the current distributions on both the active and parasitic elements are calculated from Hallen‐type integral equations in the frequency range of 3 to 6 GHz. Comparing the current distributions with those of a two‐wire SA and a four‐wire four‐terminal fed SA, we study the mechanism of polarization control. It is found that the present antenna operates as a linearly polarized element at lower frequencies and a circular polarization element at higher frequencies. It is found also that the input impedance does not change appreciably in frequency and hence the broadband nature of the conventional two‐wire SA is retained. Finally, it is confirmed numerically that circular polarizations in the opposite sense can be obtained over a wide range of angle due to change in excitation phase when four arms are excited.
Donor‐Reactivity‐Controlled Sialylation Reactions (2021)

Abstract Although tremendous efforts have been made for the efficient preparation of sialosides, controlling the stereochemical outcome of sialylation reaction still remains one of the most challenging tasks due to the unique chemical structure of sialic acid. We developed a new strategy to statistically analyze the stereoselectivity of sialylation reactions on six types of p ‐tolyl thiosialosides in NIS/TfOH system using Relative Reactivity Value (RRV) as the indicator. Analysis of the reaction mechanism showed the formation of the relatively stable glycosyl bromide and glycosyl chloride intermediates from halide‐ and triflate‐containing promotors in the absence of an acceptor. We found that the α/β‐stereoselectivity, yields, and intermediate changes were associated with their donor reactivity. These findings enable to tailor the most suitable building blocks for stereo‐controlled sialylation reactions.
Decoding the evolution of a breast cancer genome (2009)

Shah et al have recently reported the first successful sequencing of the entire genome of a solid tumour (Shah et al, 2009). Philippe Bedard and Christos Sotiriou analyse their findings as well as the challenges of applying the study of cancer genomes to clinical cancer care.
Risk Factors for Being Seronegative following SARS-CoV-2 Infection in a Large Cohort of Health Care Workers in Denmark (2021)

Most individuals seroconvert after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but being seronegative is observed in 1 to 9%. We aimed to investigate the risk factors associated with being seronegative following PCR-confirmed SARS-CoV-2 infection. In a prospective cohort study, we screened health care workers (HCW) in the Capital Region of Denmark for SARS-CoV-2 antibodies. We performed three rounds of screening from April to October 2020 using an enzyme-linked immunosorbent assay (ELISA) method targeting SARS-CoV-2 total antibodies. Data on all participants' PCR for SARS-CoV-2 RNA were captured from national registries. The Kaplan-Meier method and Cox proportional hazards models were applied to investigate the probability of being seronegative and the related risk factors, respectively. Of 36,583 HCW, 866 (2.4%) had a positive PCR before or during the study period. The median (interquartile range [IQR]) age of 866 HCW was 42 (31 to 53) years, and 666 (77%) were female. After a median of 132 (range, 35 to 180) days, 21 (2.4%) of 866 were seronegative. In a multivariable model, independent risk factors for being seronegative were self-reported asymptomatic or mild infection hazard ratio (HR) of 6.6 (95% confidence interval [CI], 2.6 to 17; <i>P</i> < 0.001) and body mass index (BMI) of ≥30, HR 3.1 (95% CI, 1.1 to 8.8; <i>P</i> = 0.039). Only a few (2.4%) HCW were not seropositive. Asymptomatic or mild infection as well as a BMI above 30 were associated with being seronegative. Since the presence of antibodies against SARS-CoV-2 reduces the risk of reinfection, efforts to protect HCW with risk factors for being seronegative may be needed in future COVID-19 surges. <b>IMPORTANCE</b> Most individuals seroconvert after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but negative serology is observed in 1 to 9%. We found that asymptomatic or mild infection as well as a BMI above 30 were associated with being seronegative. Since the presence of antibodies against SARS-CoV-2 reduces the risk of reinfection, efforts to protect HCW with risk factors for being seronegative may be needed in future COVID-19 surges.
:1009-9727(2006)02-333-01 Analysis of resistance of 175 methicillin-resistant Staphylococcus aureus to ten antibiotics (2006)

Objective To analyze resistance of 175 methicillinresistant Staphylococcus aureus(MRSA) to rifampicin and other nine antibiotics,and provide guidance for clinical use of the drug in reasonable way. Methods K-B scrip was used for testing the sensitivity of ten antibiotics including rifampicin. Results The sensitivity of methicillin-resistant Staphylococcus aureus to rifampicin was just lower than that of vancomycin,but higher than that of other eight antibiotics.Moreover,the difference is significant(P0.01). Conclusion Rifampicin can,based on the severity of illness,be chosen for treatment of methicillin-resistant Staphylococcus aureus.
Transforming growth factor-β-activated kinase 1 enhances H2O2-induced apoptosis independently of reactive oxygen species in cardiomyocytes (2013)

Our data suggest that HSP70 protects cardiomyocytes from apoptosis under oxidative stress through downregulation of intracellular ROS generation and inhibition of p38 phosphorylation. Although TAK1 itself has no effect on intracellular ROS accumulation, it may affect the inhibitory effects of HSP70 on ROS generation, p38 activity and cardiomyocyte injury.
An Ultraviolet Micro-Raman Spectrometer: Resonance Raman Spectroscopy within Single Living Cells (1990)

Ultraviolet-excited resonance Raman spectra have been obtained for the first time for microsamples of dAMP deoxymononucleotide and DNA and for the nuclear DNA of a single living T47D cultured cell. The spectral variations observed in changing the excitation wavelength reflect the dramatic variations of the Raman cross sections of the various DNA compounds in the ultraviolet region. Many of the spectral features of the single-cell nuclear DNA excited by 257-nm radiation essentially arise from cellular purines, although a contribution from the protein amide I band and from the tryptophan residues is probably observed in the 1600–1700 cm −1 spectral region.
Monitored Execution of Robot Plans Produced by Strips (1971)

We describe PLANEX1, a plan executor for the Stanford Research Institute robot system. The problem-solving program STRIPS creates a plan consisting of a sequence of actions, and PLANEX1 program carries out the plan by executing the actions. PLANEX1 is designed so that it executes only that portion of the plan necessary for completing the task, reexecutes any portion of the plan that has failed to achieve the desired results, and initiates replanning in situations where the plan can no longer be effective in completing the task. The scenario for an example plan execution is given.
Letter to the Editor (1983)

Smeby, Robert R. Ph.D.; Sen, Subha Ph.D., D.S.c; Husain, Ahsan Ph.D.; Brosnihan, K Bridget Ph. D. Author Information
Staurosporine, a non‐specific PKC inhibitor, induces keratinocyte differentiation and raises intracellular calcium, but Ro31–8220, a specific inhibitor, does not (1994)

The responsiveness of normal human keratinocytes to different modulators of protein kinase C (PKC) was investigated. The PKC agonist TPA, staurosporine (a non-specific inhibitor), and Ro31-8220 (a specific inhibitor) were studied for effect on cell morphology, growth rate, involucrin expression, and intracellular calcium levels. Surprisingly the response to nanomolar concentrations of staurosporine was similar to TPA and induced a fusiform morphology, inhibited growth, increased involucrin levels, and raised intracellular calcium. Staurosporine also increased the number of cornified envelopes, and its action therefore appeared identical to TPA. In contrast, Ro31-8220 had little effect on morphology or growth and blocked both the TPA-induced growth inhibition and calcium rise. Ro31-8220 had no effect on staurosporine-induced growth inhibition but partially reduced its associated calcium rise. These results suggest PKC activation is required for keratinocyte differentiation and that staurosporine acts like a PKC agonist to give a similar effect as TPA. Specific inhibition of PKC by Ro31-8220 inhibits TPA-induced differentiation.
Supporting personal photo storytelling for social albums (2010)

Information overload is one of today's major concerns. As high-resolution digital cameras become increasingly pervasive, unprecedented amounts of social media are being uploaded to online social networks on a daily basis. In order to support users on selecting the best photos to create an online photo album, attention has been devoted to the development of automatic approaches for photo storytelling. In this paper, we present a novel photo collection summarization system that learns some of the users' social context by analyzing their online photo albums, and includes storytelling principles and face and image aesthetic ranking in order to assist users in creating new photo albums to be shared online. In an in-depth user study conducted with 12 subjects, the proposed system was validated as a first step in the photo album creation process, helping users reduce workload to accomplish such a task. Our findings suggest that a human audio/video professional with cinematographic skills does not perform better than our proposed system.