1407 BIOFABRICATION OF INNERVATED MUSCLE TISSUE FOR ACCELERATED RESTORATION OF MUSCLE FUNCTION (2013)

You have accessJournal of UrologyTechnology & Instruments: Robotics: Benign & Malignant Disease (II)1 Apr 20131407 BIOFABRICATION OF INNERVATED MUSCLE TISSUE FOR ACCELERATED RESTORATION OF MUSCLE FUNCTION Hyun-Wook Kang, In Kap Ko, Cheil Kim, Sang Jin Lee, Anthony Atala, and James Yoo Hyun-Wook KangHyun-Wook Kang Winston Salem, NC More articles by this author , In Kap KoIn Kap Ko Winston Salem, NC More articles by this author , Cheil KimCheil Kim Winston Salem, NC More articles by this author , Sang Jin LeeSang Jin Lee Winston Salem, NC More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , and James YooJames Yoo Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.2761AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Building a clinically applicable muscle tissue with necessary tissue organization and function would be a major advance in reconstructive surgery. To achieve functional recovery in vivo, bioengineered muscle tissues must be integrated with the host nervous system following implantation. Failure of innervation results in muscle tissue atrophy. Therefore, timely integration of host nerve into bioengineered muscle tissue is critical to successful recovery of function. To overcome these limitations, we aimed to develop organized muscle tissue constructs with neuromuscular junction in vitro and to test innervation efficiency of the muscle construct in vivo. METHODS An integrated organ printing system was developed to print implantable tissue structures using a 3-D CAD model. Muscle tissue constructs were generated by printing muscle cells (C2C12 cells; 2ü∼106 cells/ml) suspended in a mixture of gelatin (40 mg/ml), hyaluronic acid (3 mg/ml), glycerol (10% v/v) and fibrinogen (30 mg/ml), followed by addition of cold thrombin (20∼30 U/ml) for 30 minutes for fibrin gel formation. The bioprinted muscle constructs were cultured to induce differentiation of muscle cells into muscle fibers, and then treated with agrin to pre-pattern acetylcholine receptors on the fibers. The muscle constructs were integrated with mobilized host sciatic nerve and implanted subcutaneously (ectopically) in nude rats. The constructs were retrieved and examined with histological (H&E) and immunohistological methods (MHC and alpha-BTX) at different time points. RESULTS The bioprinted muscle tissue constructs maintained their structural organization, consisting of viable muscle fiber bundles in culture. More importantly, the fiber bundles expressed muscle-specific characteristics after induction of cellular differentiation as well as acetylcholine receptor (AChR) expression by agrin treatment. The retrieved muscle constructs showed well-organized muscle fiber structures expressing AChRs and evidence of neural integration with host sciatic nerve. CONCLUSIONS Our findings show that tissue constructs generated with an integrated organ printer are able to maintain structural and functional characteristics of muscle in vitro and in vivo. The results of this study suggest that creation of innervated volumetric bioengineered muscle using an organ printing system is possible and that muscle function can be recovered in an accelerated fashion. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e576 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Hyun-Wook Kang Winston Salem, NC More articles by this author In Kap Ko Winston Salem, NC More articles by this author Cheil Kim Winston Salem, NC More articles by this author Sang Jin Lee Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author James Yoo Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...