gebhart/patrepeval huggingface dataset:
Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide from Ilex asprella for Sarcoma Immunotherapy (2018)

We report here the discovery of an acidic polysaccharide, namely IAPS-2, from the root of <i>Ilex asprella</i>, with anti-tumor activity via a repolarizing tumor associated macrophages (TAMs) phenotype. We obtained IAPS-2 polysaccharide from this herb based on acidity and found that IAPS-2 expressed the activity of promoting the secretion of anti-tumor cytokines in macrophages. Furthermore, we evaluated its anti-tumor effect on TAM cells, through the activation of nuclear factor-κB (NF-κB) and signal transducer and activator of transcription (STAT) signaling. In particular, in the tumor murine model, IAPS-2 demonstrated that it could significantly inhibit the growth of tumors via modulating the function of TAMs and increase the animal survival rate. In summary, IAPS-2, with a clearly illustrated chemical composition, potent anti-tumor activity, and a solid mechanism of action, may be developed into a valuable therapeutic tool for cancer immunotherapy.
Pressure losses in non-Newtonian flow through rigid wall tapered tubes (1987)

Pressure drop and pressure gradient were measured in steady Newtonian and non-Newtonian flow through tapered tubes having angles of taper, alpha, between 0.5 degree and 1.25 degrees. Aqueous solutions of polyacrylamide, characterized as power law fluids, were used for the non-Newtonian flow measurements. These solutions had power law parameters similar in magnitude to those of blood. The pressure drop-flow rate data compared well with the predictions of a semi-empirical flow model over a large range of flow rates (Re alpha up to 10 for Newtonian flow and 5.7 for non-Newtonian flow). The pressure gradient increased with distance, z, into the taper as the radius decreased. The linear relationship between pressure gradient and z, derived by Oka (Biorheology, 10, 207-212, 1973) was found to be valid only when alpha z was small. For the tapered tubes examined here, agreement was confined to the region near the inlet. If higher order terms in alpha z were taken into account the agreement was extended further into the taper. However, under higher flow conditions, when the inertial losses are not negligible, the semi-empirical model provides much better estimates of pressure gradient.
Impact of work-related psychosocial factors on mental health: A cross-sectional study in the French working population (2019)

International audience
Long‐term efficacy and safety of low‐dose ritonavir‐boosted atazanavir (ATV/r) 200/100 mg in HIV‐infected Thai patients (2010)

7‐11 November 2010, Tenth International Congress on Drug Therapy in HIV Infection, Glasgow, UK
Capturing the dynamic association between a tris-dipicolinate lanthanide complex and a decapeptide: a combined paramagnetic NMR and molecular dynamics exploration (2021)

In the realm of biomolecules, peptides can present a large diversity of structures. Our study sheds new light on the structural interplay between a tris-dipicolinate lanthanide probe and a decapeptide SASYKTLPRG. Although a rather trivial, electrostatically driven interaction was expected, the combination of paramagnetic NMR and molecular dynamics simulations reveals a highly dynamic association process and allows for providing extensive insights into the interaction sites and their occupancy. This study highlights the importance of a large conformational sampling to reconcile characteristic time in NMR with molecular dynamics simulations, where sampling in the microsecond range is needed. This study opens the door for a detailed mechanistic elucidation of the early steps of lanthanide complex-peptide or lanthanide complex-protein interaction or self-assembly processes.
Coherent Temporal Phase Transfer in Backward Wave Parametric Oscillator at 1.4 µm (2019)

The frequency modulation transfer property of a backward wave optical parametric oscillator is employed to generate compressed near-IR pulses at 1.4µm with 1.3ps duration. Limitations to the linear frequency modulation transfer in BWOPO are investigated.
Transcutaneous Optical Measurement of Hyperbilirubinemia in Neonates (1992)

Bilirubin, a yellow pigment, is formed by the breakdown of hemoglobin. Neonates are susceptible to high bilirubin levels in their blood which places them at risk of neuronal damage, and monitoring of the bilirubin levels in these neonates is clinically required. Transcutaneous optical monitoring of the bilirubin will provide a non-invasive, inexpensive measurement of bilirubin in the skin.\nThe optical properties of skin are important for interpretation of the reflected light from the skin. In this report, the optical properties of neonatal skin were measured in the visible range on twenty in vitro skin samples. The scattering of the skin is dominated by the collagen fiber bundles in the dermis. Scattering in the dermis increases linearly with gestational maturity due to the accompanying increase in the size and number of the collagen fiber bundles. Scattering in the dermis was modelled by Mie and Rayleigh scattering.\nThe collection efficiency of an optical patch used for reflectance measurements at the skin surface varies with the skin's optical properties. The collection efficiency of the optical patch as a function of optical properties was determined by measurements in phantoms, and by Monte Carlo computer models. Dermal absorbers and epidermal melanin affect the reflected signals differently, and have to be analyzed separately.\nIn addition to bilirubin content of neonatal skin, other sources of variation include skin maturity, skin thickness, melanin content, blood depth, and blood content. Each of these factors affects the reflected spectrum. Each source of variation was analyzed individually and an algorithm was developed to determine the absorbances of bilirubin and blood in the dermis from optical patch reflectance measurements.\nThe algorithm was applied to analyze reflectance measurements performed on a heterogeneous clinical population consisting of 47 neonates. The algorithm was then adjusted to minimize a score designed to ensure that the determined in vivo cutaneous bilirubin concentrations were invariable with skin melanin and blood content. Consideration of optical transport in the skin has enabled the determination of cutaneous bilirubin concentration in heterogeneous neonatal populations.
The NASA-ISRO SAR mission - An international space partnership for science and societal benefit (2015)

The National Aeronautics and Space Administration (NASA) in the United States and the Indian Space Research Organisation (ISRO) have embarked on the formulation of a proposed Earth-orbiting science and applications mission that would exploit synthetic aperture radar to map Earth's surface every 12 days. The mission's primary objectives would be to study Earth land and ice deformation, and ecosystems, in areas of common interest to the US and Indian science communities. To meet demanding coverage, sampling, and accuracy requirements, the system would require a swath of over 240 km at fine resolution, using full polarimetry where needed. To address the broad range of disciplines and scientific study areas of the mission, a dual-frequency system was conceived, at L-band (24 cm wavelength) and S-band (10 cm wavelength). To achieve these observational characteristics, a reflector-feed system is considered, whereby the feed aperture elements are individually sampled to allow a scan-on-receive (“SweepSAR”) capability at both L-band and S-band. In the partnership, NASA would provide the instrument structure for both L- and S-band electronics, the L-band electronics, the reflector and associated boom, and an avionics payload to interface with the radar that would include a solid state recorder, high-rate Ka-band telecommunication link, and a GPS receiver. ISRO would provide the spacecraft and launch vehicle, and the S-band radar electronics.
8.4.2 Enabling Changes in Systems throughout the Entire Life‐Cycle – Key to Success ? (2000)

ABSTRACT In the past decades the world has been changing in almost every aspect. Systems development is facing rapidly changing and increasingly global environments in markets, competition, technology, regulatory and societal systems. Systems to be delivered must be designed not only to meet customer or market needs, but increasingly to meet requirements and constraints of systems sharing its operational context and throughout their entire lifecycle. The design of a system must provide for a continuous evolution of its architecture either by upgrading a system already in service or releasing a new version. Based on these key challenges imosed on development systems, this paper will evolve the idea of incorporating changeability into a systems architecture. Flexibility, agility, robustness, and adaptability as four key aspects of changeability will be defined and described. Design principles to enable flexibility, agility, robustness, and adaptability within integrated systems are proposed and described. A measurement of a degree of implementation of certain aspects of changeability as well as cost associated with it are addressed with metrics. A basic process outlining and guiding an application of the framework described concludes this paper. Examples from varying industries will illustrate the applicability and implementation of selected principles. Thus this paper spans a view from why, when and where, what , and how changeability has to be incorporated into a systems architecture.
Transcriptome RNA Sequencing Data Set of Gene Expression in Moraxella catarrhalis On- and Off-Phase Variants of the Type III DNA Methyltransferase ModM3 (2020)

<i>Moraxella catarrhalis</i> is a leading bacterial cause of otitis media and exacerbations of chronic obstructive pulmonary disease. Here, we announce a transcriptome RNA sequencing data set detailing global gene expression in two <i>M. catarrhalis</i> CCRI-195ME variants with expression of the DNA methyltransferase ModM3 phase varied either on or off.
Effect of gamma sterilization on the properties of microneedle array transdermal patch system (2020)

Soluble microneedles (MNs) of four different hydrophilic polymers namely sodium carboxymethyl cellulose <b>(</b>CMC), polyvinylpyrrolidone (PVP) K30, PVP K90 and sodium hyaluronate (HU) were fabricated by mold casting technique. When exposed to gamma radiation, a dose of 25 kilogray (kGy) was found to render the microneedle (MN) sterile. However, CMC was found to form MNs with poor mechanical properties, whereas PVP K30 MNs were drastically deformed upon exposure to applied dose as observed in bright field microscopy. Scanning electron microscopy (SEM) revealed that morphology of PVP K90 and HU MNs were not significantly affected at the applied dose. The appearances of characteristic peaks of irradiated MNs of PVP K90 and HU in Fourier-transform infrared spectra suggested structural integrity of the polymers on irradiation. Differential scanning calorimetry (DSC) indicated gamma irradiation failed to alter the glass transition temperature and thus mechanical properties of PVP K90 MNs. However, DSC and Powder X-ray Diffraction (PXRD) conclusively indicated that the degree in crystallinity of HU was substantially reduced on irradiation. <i>In vitro</i> dissolution profiles of sterile PVP K90 and HU MNs were similar to un-irradiated MNs with a similarity factor (<i>f<sub>2</sub></i>) of 64 and 54, respectively. <i>In vivo</i> dissolution studies in human subjects indicated that sterile MNs of PVP K90 and HU exhibited dissolution of 78.45 ± 1.09 and 78.57 ± 0.70%, respectively, after 20 min. The studies suggested that PVP K90 and HU could be suitable polymers to fabricate soluble MNs as the structural, morphological, microstructural and dissolution properties remained unaltered post γ sterilization.
Biologic and Protective Properties of the 69-kDa Outer Membrane Protein of Bordetella pertussis: A Novel Formulation for an Acellular Pertussis Vaccine (1991)

A combination of the 69-kDa outer membrane protein and filamentous hemagglutinin (FHA), both isolated from lymphocytosis promoting factor (LPF; pertussis toxin) minus mutants of Bordetella pertussis, is protective in the mouse intracerebral challenge potency (Kendrick) test. A combination of the same 69-kDa protein and LPF is approximately 15 times less effective. These data suggest that, surprisingly, the 69-kDa protein in tandem with FHA is the most relevant combination for mouse protection; consequently such a combination may be a more suitable acellular pertussis vaccine candidate than the LPF/FHA combinations, which have never been satisfactorily protective in the mouse test. Preparation of samples of the 69-kDa protein of acceptable protective quality remains difficult. Attempts were made to screen the most suitable batches of the preparations by exploiting some recently discovered properties of the 69-kDa protein: the characteristic chromatofocusing pattern of the protein, the affinity for lymphocytes, and the ability to bind to nicotinamide adenine dinucleotide. None of these tests was able to replace the mouse intracerebral challenge potency test for final quality assessment.
Tubercular Prosthetic Knee Joint Infection (2013)

This article reports a rare case of late-onset tubercular infection following total knee arthroplasty in a 60-year-old woman who had been treated with a full course of antitubercular therapy for latent tuberculosis almost 25 years ago. Because no clear guidelines exist for optimal management of disease reactivation in the prosthetic knee joint, the authors performed a literature review and attempted to provide a treatment algorithm based on the time of presentation. Two modes of presentation were identified for unsuspected tubercular prosthetic joint infections based on the onset of symptoms and time to diagnosis: an early-onset presentation that occurs within the first 6 to 8 weeks postoperatively and a late-onset presentation that usually manifests after 8 weeks. Early-onset presentations often can be treated with standard antitubercular chemotherapy alone, whereas late-onset presentation may require 2-stage reimplantation under cover of antitubercular medications. This report also highlights the fact that despite adequate treatment of latent tuberculosis, patients may remain at risk of prosthetic joint infection following total knee arthroplasty.
Gamma‐irradiation does not impair ATRA‐induced maturation of myeloid leukaemic cells: implication for combined radiation and differentiation therapy (1998)

In the present study we investigated the effects of various doses of gamma-irradiation, followed by induction of granulocytic differentiation with all-trans-retinoic acid (ATRA), on proliferative rate, differentiation capability and oxidative metabolism of leukaemic cells from two different myeloid leukaemia cell lines, HL-60 and PLB-985. Regarding the effects of such combined treatment on the proliferative capabilities of HL-60 and PLB-985 cell lines, we showed that their growth kinetics were similar after 2 Gy gamma-irradiation combined with ATRA. However, with doses >2 Gy, the behaviour of the cell lines differed largely. Indeed, HL-60 appeared to be more radiosensitive than PLB-985 regarding cell viability and proliferation. Besides, whatever dose of irradiation (2, 5 or 10 Gy) was applied, ATRA was still able to induce differentiation of HL-60 and PLB-985 into granulocytes that retained the capacity to produce superoxide anion. The results of these in vitro studies suggest that leukaemia cell lines retain their ability to respond to ATRA, a granulocytic-differentiating inducer following high doses of irradiation. This may have implications for the use of radiation therapy in combination with ATRA for the treatment of extramedullary infiltrations of myeloid leukaemias in humans.
Refractive index change dominates the transient absorption response of metal halide perovskite thin films in the near infrared (2019)

Perovskites have lately attracted a lot of attention as promising materials for the next-generation of efficient, low-cost, and solution processable optoelectronics. Their complex transient photophysics, in time scales ranging from femtoseconds to seconds, have been widely investigated. However, in most of the reported works the spectral window of ultrafast transient absorption (TA) spectroscopy of perovskite films is limited to the visible region, hence missing crucial information coming from the near-infrared (NIR). Furthermore, the measured TA responses are affected by light interference in a thin perovskite layer making data interpretation a challenge even in the visible part of the spectrum. Here, we demonstrate a method that allows us to separately obtain the changes in absorption and refractive index from conventional transmission and reflection pump-probe measurements. We show that the contribution of the absorption change to the response of metal halide perovskite thin films in the NIR is much smaller than that of the refractive index change. Furthermore, the spectral shape of TA responses in the NIR range is predominantly determined by perovskite layer thickness and its refractive index. However, the time profile of the responses bears important information on the carrier dynamics and makes the NIR a useful range to study perovskite photophysics.
Characterization of rhoGAP. A GTPase-activating protein for rho-related small GTPases. (1994)

GTPase-activating proteins or GAPs play an important role in signal transduction pathways regulated by GTP-binding proteins. In addition to acting as down-regulators of GTPases, there is growing evidence that they also act as effector molecules required for downstream signaling. PLC-beta 1, the target protein regulated by the heterotrimeric GTPase Gq, has been shown to be a GAP, whereas rasGAP, a down-regulator of the small GTPase ras, may be required for the ras-mediated signals. We have purified a GAP specific for the rho subfamily of small GTPases. Partial sequence analysis of rhoGAP has led to the identification of a family of related proteins which now includes bcr, chimaerin, p190, p85, and 3BP-1. We report here the isolation of a cDNA clone encoding human rhoGAP and the expression of recombinant protein. The full-length protein is 50 kDa and is ubiquitously expressed in mammalian cells. At least three members of the rho family are substrates for rhoGAP, rho, rac, and G25K/CDC42, and they each bind equally well to the protein. In vitro GTPase assays, however, reveal that G25K/CDC42 is the preferred substrate. RhoGAP contains a proline-rich sequence, suggesting that it is an SH3-binding protein.
Newly Generated CD4+ T Cells Acquire Metabolic Quiescence after Thymic Egress (2017)

Mature naive T cells circulate through the secondary lymphoid organs in an actively enforced quiescent state. Impaired cell survival and cell functions could be found when T cells have defects in quiescence. One of the key features of T cell quiescence is low basal metabolic activity. It remains unclear at which developmental stage T cells acquire this metabolic quiescence. We compared mitochondria among CD4 single-positive (SP) T cells in the thymus, CD4<sup>+</sup> recent thymic emigrants (RTEs), and mature naive T cells in the periphery. The results demonstrate that RTEs and naive T cells had reduced mitochondrial content and mitochondrial reactive oxygen species when compared with SP thymocytes. This downregulation of mitochondria requires T cell egress from the thymus and occurs early after young T cells enter the circulation. Autophagic clearance of mitochondria, but not mitochondria biogenesis or fission/fusion, contributes to mitochondrial downregulation in RTEs. The enhanced apoptosis signal-regulating kinase 1/MAPKs and reduced mechanistic target of rapamycin activities in RTEs relative to SP thymocytes may be involved in this mitochondrial reduction. These results indicate that the gain of metabolic quiescence is one of the important maturation processes during SP-RTE transition. Together with functional maturation, it promotes the survival and full responsiveness to activating stimuli in young T cells.
Semiquantitative Analysis of Clinical Heat Stress in Clostridium difficile Strain 630 Using a GeLC/MS Workflow with emPAI Quantitation (2014)

Clostridium difficile is considered to be the most frequent cause of infectious bacterial diarrhoea in hospitals worldwide yet its adaptive ability remains relatively uncharacterised. Here, we used GeLC/MS and the exponentially modified protein abundance index (emPAI) calculation to determine proteomic changes in response to a clinically relevant heat stress. Reproducibility between both biological and technical replicates was good, and a 37°C proteome of 224 proteins was complemented by a 41°C proteome of 202 proteins at a 1% false discovery rate. Overall, 236 C. difficile proteins were identified and functionally categorised, of which 178 were available for comparative purposes. A total of 65 proteins (37%) were modulated by 1.5-fold or more at 41°C compared to 37°C and we noted changes in the majority of proteins associated with amino acid metabolism, including upregulation of the reductive branch of the leucine fermentation pathway. Motility was reduced at 41°C as evidenced by a 2.7 fold decrease in the flagellar filament protein, FliC, and a global increase in proteins associated with detoxification and adaptation to atypical conditions was observed, concomitant with decreases in proteins mediating transcriptional elongation and the initiation of protein synthesis. Trigger factor was down regulated by almost 5-fold. We propose that under heat stress, titration of the GroESL and dnaJK/grpE chaperones by misfolded proteins will, in the absence of trigger factor, prevent nascent chains from emerging efficiently from the ribosome causing translational stalling and also an increase in secretion. The current work has thus allowed development of a heat stress model for the key cellular processes of protein folding and export.
BIOPROCESS OPTIMIZATION AND CHARACTERIZATION OF RECOMBINANT URATE OXIDASE EXPRESSED IN Escherichia coli (2013)

Tumor Lysis Syndrome and Gout are the conditions in which uric acid levels in the serum will be increased up to 526 mg/dl but in a healthy human being the uric acid concentration is less than 15mg/dl. In such conditions urate oxidase can be administered parentrally which can solublise the uric acid in to allontoin which has 3 to 5 fold more solubility than uric acid and it can be excreted through urine and maintain the uric acid concentration at normal level. In this article urate oxidase gene was isolated Aspergillus flavus and it was cloned in to E.coli (BL-21). Urate oxidase gene was expressed in the cytoplasm as soluble and biologically active form. Bioprocess was optimized for 20lts fermenter scale with modified LB medium and purified up to&amp;gt;96% purity using fractionated ammonium sulphate precipitation, diafiltration, anion exchange chromatography, cation exchange chromatography and gel filtration chromatography. The final yield of purified recombinant urate oxidase from the 20lts fermenter was approximately 5 to 6gm of 96 % pure and biologically active enzyme.
A model predictive control framework for industrial turbodiesel engine control (2008)

This article describes the development and implementation of a practical explicit model predictive control (MPC) approach that allows subcontrollers to receive and accommodate time-varying setpoints and constraints from higher levels in standard industrial automotive controller hierarchies. The MPC approach requires a small computational footprint that is suitable for implementation within a modern electronic control unit (ECU). This article presents the approach, method of implementation, and preliminary results on a 2.2 litre turbodiesel engine.