gebhart/patrepeval huggingface dataset:
Expression of Antibody Fragments by Periplasmic Secretion in <I>Escherichia coli</I> (2005)

Antibody-based drugs are increasingly used in the clinic, and their importance is set to escalate in the coming years as more drugs in this class progress through clinical trials. Although many such drugs utilize whole antibodies, others exploit fragments, e.g., fragment antigen binding (Fab′) or single-chain fragment variable (scFv), which retain the antigen-binding specificity without the fragment crystallizable (Fc) element necessary to mediate effector functions. Antibody fragments can be advantageous for many therapeutic uses, owing to the fact that valency and half-life can be tailored through protein engineering approaches to suit the desired mechanism of action (). Furthermore, antibody fragments are more suited to expression in microbial systems, providing benefits in terms of increased scale and ease of manufacture ().
ДИНАМИКА ПОКАЗАТЕЛЕЙ ВАРИАБЕЛЬНОСТИ РИТМА СЕРДЦА У ПАЦИЕНТОВ С ПАРОКСИЗМАЛЬНОЙ ФОРМОЙ ФИБРИЛЛЯЦИИ ПРЕДСЕРДИЙ ПРИ ЛЕЧЕНИИ АТОРВАСТАТИНОМ (2008)

В исследование включено 60 больных ИБС, страдающих пароксизмальной формой ФП. Пациенты были разделены на 2 группы – груп -па сравнения (на базовой антиаритмической терапии) и группа активного лечения (базовая антиаритмическая терапия с дополнительным назначением атор -вастатина Липтонорм (ОАО Фармстандарт Лексредства, Россия) в дозе 10 мг/сут). Cравнивали показатели ВРС в двух группах исходно и через 3 мес лече -ния.
Inactivation of human and simian rotaviruses by ozone (1987)

The inactivation of simian rotavirus SA-11 and human rotavirus type 2 (Wa) by ozone was compared at 4 degrees C by using single-particle virus stocks. Although the human strain was clearly more sensitive, both virus types were rapidly inactivated by ozone concentrations of 0.25 mg/liter or greater at all pH levels tested. Comparison of the virucidal activity of ozone with that of chlorine in identical experiments indicated little significant difference in rotavirus-inactivating efficiencies when the disinfectants were used at concentrations of 0.25 mg/liter or greater.
Nonenzymatic hydrolysis of oligoribonucleotides (1992)

Selective cleavage of phosphodiester bonds in RNA is important in the processing of large RNA molecules. This paper reports specific cleavage at UA sequences in single stranded oligoribonucleotides as short as hexamers. The hydrolysis between U and A leaves a 2',3'-cyclic phosphate on the 5'-side and a 5'-hydroxyl group on the 3' side of the cleavage. The hydrolysis is promoted by a wide range of cofactors, including polymeric organic compounds such as polyvinylpyrrolydone (PVP) and by proteins. A variety of experiments suggests the cleavage is not due to contamination by ribonuclease. The rate of cleavage is a function of oligoribonucleotide, PVP and spermidine concentrations. Mg2+ is not required. The phenomenon described here can potentially provide a relatively simple way of coding chemical stability into single stranded RNA based on its sequence and structure. This process seems to be similar to that involved in post-transcriptional degradation of mRNA.
Sulfur-Containing Analogues of the Reactive [CuOH]2+ Core (2021)

With the aim of drawing comparisons to the highly reactive complex LCuOH (L = bis(2,6-diisopropylphenylcarboxamido)pyridine), the complexes [Bu<sub>4</sub>N][LCuSR] (R = H or Ph) were prepared, characterized by spectroscopy and X-ray crystallography, and oxidized at low temperature to generate the species assigned as LCuSR on the basis of spectroscopy and theory. Consistent with the smaller electronegativity of S versus O, redox potentials for the LCuSR<sup>-/0</sup> couples were ∼50 mV lower than for LCuOH<sup>-/0</sup>, and the rates of the proton-coupled electron transfer reactions of LCuSR with anhydrous 1-hydroxy-2,2,6,6-tetramethyl-piperidine at -80 °C were significantly slower (by more than 100 times) than the same reaction of LCuOH. Density functional theory (DFT) and time-dependent DFT calculations on LCuZ (Z = OH, SH, SPh) revealed subtle differences in structural and UV-visible parameters. Further comparison to complexes with Z = F, Cl, and Br using complete active space (CAS) self-consistent field and localized orbital CAS configuration interaction calculations along with a valence-bond-like interpretation of the wave functions showed differences with previously reported results ( <i>J. Am. Chem. Soc.</i> 2020, 142, 8514), and argue for a consistent electronic structure across the entire series of complexes, rather than a change in the nature of the ligand field arrangement for Z = F.
Investigating Pervaporation as a Process Method for Concentrating Formic Acid Produced from Carbon Dioxide (2020)

New methods in lowering energy consumption costs for evaporation and concentration are needed in many commercial chemical processes. Pervaporation is an underutilized, low-energy processing method that has a potential capability in achieving lower energy processing costs. A recently developed new electrochemical process that can generate a 5–25 wt% pure formic acid (FA) from the electrochemical reduction of CO2 requires a low-energy process for producing a more concentrated FA product for use in both on-site and commercial plant applications. In order to accomplish this, a 25 cm2 membrane area pervaporation test cell was constructed to evaluate the FA-H2O system separation performance of three distinct types of membrane candidates at various FA feed concentrations and temperatures. The selection included one cation ion exchange, two anion ion exchange, and two microporous hydrophobic membranes. The permeation flux rates of FA and H2O were measured for FA feed concentrations of 10, 20, 40, and 60 wt% at corresponding temperatures of 22, 40, and 60 °C. The separation performance results for these particular membranes appeared to follow the vapor liquid equilibrium (VLE) characteristics of the vapor phase in the FA-H2O system as a function of temperature. A Targray microporous hydrophobic high-density polyethylene (HDPE) membrane and a Chemours Nafion® N324 membrane showed the best permeation selectivities and mass flux rates FA feed concentrations, ranging from 10 to 40 wt%. The cation and anion ion exchange membranes evaluated were found not to show any significant enhancements in blocking or promoting the transport of the formate ion or FA through the membranes. An extended permeation cell run concentrated a 10.12% FA solution to 25.38% FA at 40 °C. Azeotropic distillation simulations for the FA-H2O system using ChemCad 6.0 were used to determine the energy requirement using steam costs in processing FA feed concentrations ranging from 5 to 30 wt%. These experimental results indicate that pervaporation is a potentially useful unit process step with the new electrochemical process in producing higher concentration FA product solutions economically and at lower capital costs. One major application identified is in on-site production of FA for bioreactors employing new types of microbes that can assimilate FA in producing various chemicals and bio-products.
Selective tungsten filling of sub-0.25 μm trenches for the fabrication of scaled contacts and x-ray masks (1990)

Trenches in a layer of SiO2 on Si, with feature sizes down to 0.125 μm have been filled with tungsten (W) using selective chemical vapor deposition (CVD). The trenches were formed in a fashion similar to contact vias by reactive ion etching the SiO2 to the underlying silicon. The tungsten was then deposited in a cold wall CVD reactor, by silane reduction of WF6, to a thickness of 0.5 μm. The process results in good planarity and uniformity making it suitable for the fabrication of sub-0.5 μm contacts and interconnects, and for the formation of high aspect ratio absorbers in x-ray masks.
Reconstructing Individual Hand Models from Motion Capture Data (2010)

In this paper, we propose a new method of reconstructing the hand models for individuals, which include the link structure models, the homologous skin surface models and the homologous tetrahedral mesh models in a reference posture. As for the link structure model, the local coordinate system related to each link consists of the joint rotation center and the axes of joint rotation, which can be estimated based on the trajectories of optimal markers on the relative skin surface region of the subject obtained from the motion capture system. The skin surface model is defined as a three-dimensional triangular mesh, obtained by deforming a template mesh so as to fit the landmark vertices to the relative marker positions obtained motion capture system. In this process, anatomical dimensions for the subject, manually measured by a caliper, are also used as the deformation constraints.
Derivation of Force Field Parameters for SnO2−H2O Surface Systems from Plane-Wave Density Functional Theory Calculations (2006)

Plane-wave density functional theory (DFT-PW) calculations were performed on bulk SnO2 (cassiterite) and the (100), (110), (001), and (101) surfaces with and without H2O present. A classical interatomic force field has been developed to describe bulk SnO2 and SnO2-H2O surface interactions. Periodic density functional theory calculations using the program VASP (Kresse et al., 1996) and molecular cluster calculations using Gaussian 03 (Frisch et al., 2003) were used to derive the parametrization of the force field. The program GULP (Gale, 1997) was used to optimize parameters to reproduce experimental and ab initio results. The experimental crystal structure and elastic constants of SnO2 are reproduced reasonably well with the force field. Furthermore, surface atom relaxations and structures of adsorbed H2O molecules agree well between the ab initio and force field predictions. H2O addition above that required to form a monolayer results in consistent structures between the DFT-PW and classical force field results as well.
Plant‐Derived Natural Products Research: Recent Progress in Drug Discovery (2008)

Abstract Numerous important bioactive compounds have been, and continue to be, isolated worldwide from natural sources. These compounds include both primary and secondary metabolites isolated mainly from plants, as well as from the animal and mineral kingdoms. The recent development of new bioassay methods has facilitated progress in the BDFI (bioactivity‐directed fractionation and isolation) of many useful bioactive compounds from natural sources (1). These active principles could be developed or additionally modified to enhance the biologic profiles as clinical trials candidates. Many natural pure compounds have become medicines, dietary supplements, and other useful commercial products. This article summarizes research on many different useful compounds isolated or developed from plants with an emphasis on those discovered recently by the laboratories of the authors as antitumor and anti‐HIV clinical trial candidates.
NMR Analysis of Interactions of a Phosphatidylinositol 3‘-Kinase SH2 Domain with Phosphotyrosine Peptides Reveals Interdependence of Major Binding Sites (1996)

The interactions of the N-terminal src homology (SH2) domain (N-SH2) of the 85 kDa subunit of phosphatidylinositol 3'-kinase (PI-3K) with phosphotyrosine (ptyr) and a series of ptyr-containing peptides have been examined by NMR spectroscopy. HSQC (heteronuclear single-quantum coherence) NMR spectra of 15N-labeled SH2 were used to evaluate its interactions with ptyr-containing ligands. The ability of ligands to cause chemical shift changes was compared to their potency as competitors in in vitro binding experiments using polyoma virus middle T antigen (MT). The results suggest the interdependence of SH2 binding elements. Chemical shifts of residues involved in the ptyr binding were altered by variations of the sequence of the bound peptide, suggesting that the ptyr fit can be adjusted by the peptide sequence. Perturbations of chemical shifts of residues coordinating the methionine three residues C-terminal to the ptyr (the +3 residue) were affected by substitution in the binding peptide at +1 and vice versa. Such results show synergistic interplay between regions of the SH2 binding residues C-terminal to the ptyr.
Characterization of the Duox/DuoxA interplay (2010)

info:eu-repo/semantics/nonPublished
Abstract 1437: The Chromosome 9p21 Risk Locus is Associated with a Greater Severity of Angiographically Defined Coronary Artery Disease (2008)

Background: Large-scale genome wide association studies have demonstrated a risk locus at position 9p21 conferring a 1.3–1.5 fold risk of MI and incident CAD in Caucasian populations with an allele...
Położenie prawne więźniów w Polsce lat dziewięćdziesiątych. Uwagi obserwatora (1993)

Prawa więźnia są niekończącym się eksperymentem. Są eksperymentem w dziedzinie ludzkiej i instytucjonalnej zdolności do samoograniczania się władzy publicznej w sytuacji moralnie usprawiedliwionego ścieśnienia praw przestępcy.
An MDL-based approach to extracting subword units for grapheme-to-phoneme conversion (2010)

We address a key problem in grapheme-to-phoneme conversion: the ambiguity in mapping grapheme units to phonemes. Rather than using single letters and phonemes as units, we propose learning chunks, or subwords, to reduce ambiguity. This can be interpreted as learning a lexicon of subwords that has minimum description length. We implement an algorithm to build such a lexicon, as well as a simple decoder that uses these subwords.
Role of prostaglandin in the formation of osteoclasts induced by capsular‐like polysaccharide antigen of Actinobacillus actinomycetemcomitans strain Y4 (1995)

We found no reports that capsular-like polysaccharide antigen purified from Actinobacillus actinomycetemcomitans either induces osteoclastic bone resorption in mouse organ cultures or promotes osteoclast formation in mouse marrow cultures. In contrast, capsular-like polysaccharide antigen purified from A. actinomycetemcomitans strain Y4 induced bone resorption in mouse organ culture. To examine the mechanism of bone resorption induced by A. actinomycetemcomitans, mouse bone marrow cells were cultured with A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen. A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen stimulated osteoclast-like cell formation in mouse bone marrow cultures. However, the polysaccharide of A. actinomycetemcomitans lipopolysaccharide did not induce the formation of osteoclast-like cells. Indomethacin inhibited osteoclast-like cell formation mediated by A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen in a dose-dependent manner. There was a good correlation between the number of osteoclast-like cells formed in the marrow culture and the amount of prostaglandin E2 released into the culture media. When mouse bone marrow cells were cultured with prostaglandin E2 during the culture periods, many osteoclast-like cells were formed. These results indicate that prostaglandin E2 is involved in the mechanism of the formation of osteoclast-like cells mediated by A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen. A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen may play an important role in inflammatory bone resorption by promoting osteoclast formation in periodontal disease.
On Petri net models of infinite state supervisors (1992)

A class of supervisory control problems that require infinite state supervisors is considered, and Petri nets with inhibitor arcs (PNs) are introduced to model the supervisors. This PN-based approach to supervisory control is compared to automata-based approaches. The primary advantage of a PN-based supervisory controller is that it provides a finite representation of an infinite state supervisor. For verification, implementation, and testing reasons, a finite PN-based representation of an infinite state supervisor is preferred over an automata-based supervisor. It is shown that this modeling advantage is accompanied by a decision disadvantage, in that in general the controllability of a language that can be generated by the closed-loop system is undecidable.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
In Vitro Propagation from Axillary Buds of Glycyrrhiza glabra L. (1995)

Stem segments with axillary buds of Glycyrrhiza glabra L. were cultured on modified MS medium supplemented with α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). Formation of multiple shoots was obtained on the medium provided with 1.00mg/l BAP after 40 days of culture.Each shoot was cut and transplanted onto the medium supplemented with NAA and BAP. After 80 days of culture, the rooting of shoots was well established in the media containing 0.01-0.50mg/l NAA and these shoots subsequently developed into whole plants.
Power delivery for remotely located microsystems (2004)

Supplying power to bio-implanted devices or to devices located in a hostile environment is an important problem. In the former case, power can be supplied by means of an implanted or a local battery, or by wires, which for a bio-implanted application results in wires penetrating through skin. This work examines a wireless power delivery system for a remotely located microsystem. The external transmitter coil and the receiver coil located with the microsystem form a loosely coupled system. A conventional rectifier circuit is used for conversion of induced receiver coil ac voltage to a de voltage. For an input supply voltage of 0.35 V/sub rms/, the transmitted power to a 34 /spl Omega/ load resistor connected to the receiver coil were 4 and 13.2 mW, when the receiver coil was placed at the center and at the rim of the transmitter coil, respectively. These values are encouraging and means for further improvement are discussed.
Left Atrial Appendage Closure Devices (2014)

Atrial fibrillation (AF) increases the risk for thromboembolic stroke five-fold. The left atrial appendage (LAA) has been shown to be the main source of thrombus formation in the majority of strokes associated with AF. Oral anticoagulation with warfarin and novel anticoagulants remains the standard of care; however, it has several limitations, including bleeding and poor compliance. Occlusion of the LAA has been shown to be an alternative therapeutic approach to drug therapy. The purpose of this article is to review the different techniques and devices that have emerged for the purpose of occluding this structure, with a particular emphasis on the efficacy and safety studies published to date in the medical literature.
Hybrid broadcast broadband TV, the new way to a comprehensive TV experience (2011)

Hybrid broadcast broadband TV (HbbTV) is a European initiative for providing both broadcast and broadband/web content on a flat-panel screen in the living room. The HbbTV specification introduces only a few new technical components; it is mainly based on existing standards. In this respect, it represents a specific profile of available technologies, rather than a completely new technical development. This paper gives an overview over the architecture of HbbTV and the current status of the market introduction.
Efficacy of erbium YAG laser‐assisted autologous epidermal grafting in vitiligo (2002)

The results obtained with laser-assisted grafting are more satisfactory than those achieved with conventional grafting techniques. We found that the repigmentation zones are larger (up to 9 mm in the former vs. 3 mm in the latter) and cobblestoning does not occur with laser-assisted grafting. Also, the procedure is precise, relatively atraumatic and can be performed rapidly even when covering vast areas.
Transforming growth factor-beta and response to anticancer therapies in human liver and gastric tumors in vitro and in vivo. (2000)

Liver cancer and gastric cancer are the most common solid tumors worldwide. Transforming growth factor-beta (TGF-beta) production and lack of response to TGF-beta growth inhibitory effects have been associated with tumor progression and therapeutic resistance. HepG2, Hep3B, and SK-HEP-1 human liver cancer lines produce 3, 5.7, and 2.5 ng TGF-beta1; 1.4, 2, and 4 ng TGF-beta2 and 0.15, 0.2 and 0.22 ng TGF-beta3 per 107 cells (24 h). Expression of the TGF-beta type I receptor is 20x, 1x, and 0.6x the level in mink lung MvLu1 cells in the HepG2, Hep3B, and SK-HEP-1 cells, respectively. HepG2 and Hep3B cells do not express the TGF-beta type II receptor while SK-HEP-1 cells express 7x the level found in mink lung MvLu1 cells. Hs 746T, KATO III, RF-1, and RF-48 human gastric cancer cell lines produce 12. 5, 0.35, 0.4, and 0.4 ng TGF-beta1; 2.6, 0.95, 0.5, and 0.52 ng TGF-beta2 and 0.42, 0.17, 0.12, and 0.14 ng TGF-beta3 per 107 cells (24 h). Expression of TGF-beta type I receptor is 0.7x, 0.7x, 0.8x, 0.6x the level in mink lung MvLu1 cells in the Hs 746T, KATO III, RF-1 and RF-48 cells, respectively. KATO III cells are lacking in the TGF-beta type II receptor while Hs 746T, RF-1 and RF-48 cells express 10x, 0.8x, and 1x the levels in mink lung MvLu1 cells. The IC50 for TGF-beta1 is >>10 ng/ml in all of these lines except RF-48 where TGF-beta1 is mitogenic. The response of the cell lines to radiation, doxorubicin, mitomycin C, cisplatin, 5-fluorouracil, methotrexate, and gemcitabine showed that SK-HEP-1 was the most drug resistant liver cancer cell line and KATO III was the most drug resistant gastric cancer cell line. Overall, there was no correlation between TGF-beta secretion in cell culture and sensitivity of the cells to anticancer agents. Increased TGF-beta1 levels were detectable in the plasma of nude mice bearing Hep3B and Hs 746T xenografts. Those tumors which secreted greater amounts of TGF-beta were more therapeutically resistant in vivo.
Intermolecular Enol Ether−Alkyne Metathesis (2003)

[reaction: see text] A new intermolecular metathesis between a variety of alkynes and alkyl enol ethers is described. The best results were obtained with the second generation Grubbs' precatalysts. The reaction occurred with a high degree of regioselectivity and provided electron-rich dienes, which underwent a variety of [4 + 2] cycloadditions.
A Light-Responsive Release Platform by Controlling the Wetting Behavior of Hydrophobic Surface (2014)

Controlled release system based on mesoporous silica (MS) nanomaterials has drawn great attention over the past decades due to its potential biomedical applications. Herein, a light-responsive release system based on MS nanoparticles was achieved by adjusting the wetting of the MS surface. At the starting stage, the surface of MS modified with optimal ratio of spiropyran to fluorinated silane (MS-FSP) was protected from being wetted by water, successfully inhibiting the release of model cargo molecules, fluorescein disodium (FD). Upon irradiation with 365 nm UV light, the conformational conversion of spiropyran from a "closed" state to an "open" state caused the surface to be wetted, leading to the release of FD from the pores. The further in vitro studies demonstrated the system loaded with anticancer drug camptothecin (CPT) could be effectively controlled to release the drug by UV light stimuli to enhance cytotoxicity for EA.hy926 cells and HeLa cells. This wettability-determined smart release platform could be triggered by remote stimuli, which might hold promise in the applications of drug delivery and cancer therapy.