gebhart/patrepeval huggingface dataset:
CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum (2016)

CRISPR/Cas9 based systems have emerged as versatile platforms for precision genome editing in a wide range of organisms. Here we have developed powerful CRISPR/Cas9 tools for marker-based and marker-free genome modifications in Penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. The developed CRISPR/Cas9 toolbox is highly flexible and allows editing of new targets with minimal cloning efforts. The Cas9 protein and the sgRNA can be either delivered during transformation, as preassembled CRISPR-Cas9 ribonucleoproteins (RNPs) or expressed from an AMA1 based plasmid within the cell. The direct delivery of the Cas9 protein with in vitro synthesized sgRNA to the cells allows for a transient method for genome engineering that may rapidly be applicable for other filamentous fungi. The expression of Cas9 from an AMA1 based vector was shown to be highly efficient for marker-free gene deletions.
Synthesis and Surface Modification of TiO2-Based Photocatalysts for the Conversion of CO2 (2020)

Among all greenhouse gases, CO2 is considered the most potent and the largest contributor to global warming. In this review, photocatalysis is presented as a promising technology to address the current global concern of industrial CO2 emissions. Photocatalysis utilizes a semiconductor material under renewable solar energy to reduce CO2 into an array of high-value fuels including methane, methanol, formaldehyde and formic acid. Herein, the kinetic and thermodynamic principles of CO2 photoreduction are thoroughly discussed and the CO2 reduction mechanism and pathways are described. Methods to enhance the adsorption of CO2 on the surface of semiconductors are also presented. Due to its efficient photoactivity, high stability, low cost, and safety, the semiconductor TiO2 is currently being widely investigated for its photocatalytic ability in reducing CO2 when suitably modified. The recent TiO2 synthesis and modification strategies that may be employed to enhance the efficiency of the CO2 photoreduction process are described. These modification techniques, including metal deposition, metal/non-metal doping, carbon-based material loading, semiconductor heterostructures, and dispersion on high surface area supports, aim to improve the light absorption, charge separation, and active surface of TiO2 in addition to increasing product yield and selectivity.
Preclinical in vivo Performance of Novel Biodegradable, Electrospun Poly(lactic acid) and Poly(lactic-co-glycolic acid) Nanocomposites: A Review (2015)

Bone substitute materials have witnessed tremendous development over the past decades and autogenous bone may still be considered the gold standard for many clinicians and clinical approaches in order to rebuild and restore bone defects. However, a plethora of novel xenogenic and synthetic bone substitute materials have been introduced in recent years in the field of bone regeneration. As the development of bone is actually a calcification process within a collagen fiber arrangement, the use of scaffolds in the formation of fibers may offer some advantages, along with additional handling characteristics. This review focuses on material characteristics and degradation behavior of electrospun biodegradable polyester scaffolds. Furthermore, we concentrated on the preclinical in vivo performance with regard to bone regeneration in preclinical studies. The major findings are as follows: Scaffold composition and architecture determine its biological behavior and degradation characteristics; The incorporation of inorganic substances and/or organic substances within composite scaffolds enhances new bone formation; L-poly(lactic acid) and poly(lactic-co-glycolic acid) composite scaffolds, especially when combined with basic substances like hydroxyapatite, tricalcium phosphate or demineralized bone powder, seem not to induce inflammatory tissue reactions in vivo.
Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics (2023)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTAdvances in Microfluidics: Technical Innovations and Applications in Diagnostics and TherapeuticsGuillaume AubryGuillaume AubrySchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United StatesMore by Guillaume AubryView Biographyhttps://orcid.org/0000-0001-7828-8508, Hyun Jee LeeHyun Jee LeeSchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United StatesMore by Hyun Jee LeeView Biographyhttps://orcid.org/0000-0001-9662-2063, and Hang Lu*Hang LuSchool of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, United StatesPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, United States*Email: [email protected]More by Hang LuView Biographyhttps://orcid.org/0000-0002-6881-660XCite this: Anal. Chem. 2023, 95, 1, 444–467Publication Date (Web):January 10, 2023Publication History Published online10 January 2023Published inissue 10 January 2023https://pubs.acs.org/doi/10.1021/acs.analchem.2c04562https://doi.org/10.1021/acs.analchem.2c04562review-articleACS PublicationsCopyright © 2023 American Chemical SocietyRequest reuse permissionsArticle Views3385Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:3D printing,Biotechnology,Fluid dynamics,Liquids,Sensors Get e-Alerts
A coding method for high bit-depth images based on optimized bit-depth transform (2010)

In recent years, high bit-depth (HBD) images with 10 [bits/channel] or more are being used more often for their improved image quality. However, the size of HBD images become large and so more effective encoding techniques are needed. This paper considers the bit-depth transform process from the view point of minimizing bit-depth transform error. The minimization method is designed according to an optimum quantization algorithm. As a result, our method can improve the coding efficiency by an average of 11 [%] compared to the conventional method with AVC/H.264.
Supplementary Figure from Phase I Clinical Trial of DNA Methyltransferase Inhibitor Decitabine and PARP Inhibitor Talazoparib Combination Therapy in Relapsed/Refractory Acute Myeloid Leukemia (2023)

Supplementary Figure from Phase I Clinical Trial of DNA Methyltransferase Inhibitor Decitabine and PARP Inhibitor Talazoparib Combination Therapy in Relapsed/Refractory Acute Myeloid Leukemia
Harvesting Energy from Bridge Vibration by Piezoelectric Structure with Magnets Tailoring Potential Energy (2021)

Bridges play an increasingly more important role in modern transportation, which is why many sensors are mounted on it in order to provide safety. However, supplying reliable power to these sensors has always been a great challenge. Scavenging energy from bridge vibration to power the wireless sensors has attracted more attention in recent years. Moreover, it has been proved that the linear energy harvester cannot always work efficiently since the vibration energy of the bridge distributes over a broad frequency band. In this paper, a nonlinear energy harvester is proposed to enhance the performance of harvesting bridge vibration energy. Analyses on potential energy, restoring force, and stiffness were carried out. By adjusting the separation distance between magnets, the harvester could own a low and flat potential energy, which could help the harvester oscillate on a high-energy orbit and generate high output. For validation, corresponding experiments were carried out. The results show that the output of the optimal configuration outperforms that of the linear one. Moreover, with the increase in vehicle speed, a component of extremely low frequency is gradually enhanced, which corresponds to the motion on the high-energy orbit. This study may give an effective method of harvesting energy from bridge vibration excited by moving vehicles with different moving speeds.
52. Neurological Analysis on Surgical Indication of Hypertensive Intracerebral Hemorrhage and its Results (1971)

hemiplegia with our angiographic classification of hematoma.1) The recovery of hemiplegia was almost complete in case with hematoma localized in the putamen (external capsule) by both surgical evacuation and medical treatment.
Protein and energy requirements for maintenance of indigenous Granadina goats (1990)

Sixteen adult castrated male goats of the Granadina breed, with initial live weights ranging from 26.0 to 33.3 kg were used in two experiments to determine their protein and energy requirements for maintenance. Digestibility, nitrogen and energy balance measurements were made during the experiments. Two diets, which were based on pelleted lucerne (Medicago sativa) hay alone or on this forage and barley, were individually given at about maintenance level once daily. Gas exchange was measured using open-circuit respiration chambers. Fasting heat production was also determined. By regression analysis endogenous urinary N and maintenance requirements for N were estimated to be 119 mg/kg body-weight (W)0.75 per d and 409 mg total N/kg W0.75 per d respectively. Fasting heat production was 324 kJ/kg W0.75. The energy requirement for maintenance was calculated by regression of energy balance on metabolizable energy (ME) intake and a value of 443 kJ/kg W0.75 per d was found. The overall efficiency of utilization of ME for maintenance was 0.73.
Automatic recruitment maneuvers in porcine acute lung injury based on online PaO(2 )measurements (2006)

The individualization of lung protective ventilation strategies (e.g. recruitment maneuvers [RM] and PEEP titration to keep the lungs open) requires careful bedside observations. Many parameters must be monitored, which calls for computer aid. A fuzzy-logic ventilation expert system has been tested regarding its ability to automatically conduct RMs based on the open lung concept (OLC).
Data management in location-dependent information services (2002)

Location-dependent information services have great promise for mobile and pervasive computing environments. They can provide local and nonlocal news, weather, and traffic reports as well as directory services. Before they can be implemented on a large scale, however, several research issues must be addressed.
Multi-layer transmission line of spoof surface plasmon polaritons (2022)

In modern microwave technology, multi-layer structure is widely adopted in compact circuit design. In a multi-layer microwave system, the transmission line (TL) plays an important role. The multi-layer TLs need to have high cross-layer transmission efficiency, which is a big challenge in highly integrated circuits. Spoof surface plasmon polaritons (SSPP) possess good performance on field confinement and low transmission loss at microwave and terahertz frequencies, and can achieve the compact design in planar microwave circuits. In this article, a new type of multi-layer SSPP TL is proposed and tested. Taking advantage of the properties of SSPPs, the proposed TLs achieve high transmission efficiency for both in-layer and cross-layer situations. The proposed SSPP TLs have great prospect in the future multi-layer circuit design.
Learning Locomotion Skills Using DeepRL: Does the Choice of Action Space Matter? (2016)

The use of deep reinforcement learning allows for high-dimensional state descriptors, but little is known about how the choice of action representation impacts learning and the resulting performance. We compare the impact of four different action parameterizations (torques, muscle-activations, target joint angles, and target joint-angle velocities) in terms of learning time, policy robustness, motion quality, and policy query rates. Our results are evaluated on a gait-cycle imitation task for multiple planar articulated figures and multiple gaits. We demonstrate that the local feedback provided by higher-level action parameterizations can significantly impact the learning, robustness, and motion quality of the resulting policies.
Substantial improvements in performance indicators achieved in a PBMC cryopreservation quality assurance program using single donor samples Running title: Viability, yield and function QAP for cryopreserved PBMC (2006)

1 Storage of high quality cryopreserved peripheral blood mononuclear cells (PBMC) is 2 often a requirement for multicentre clinical trials and requires a reproducibly high 3 standard of practise. A quality assurance program (QAP) was established to assess an 4 Australia-wide network of laboratories in the provision of high quality PBMC (determined 5 by yield, viability and function) using blood taken from single donors (HIV-positive and 6 HIV-negative) and shipped to each site for preparation and cryopreservation of PBMC. 7 The aim of the QAP was to provide laboratory accreditation for participation in clinical 8 trials and cohort studies which require preparation and cryopreservation of PBMC, and 9 to assist all laboratories to prepare PBMC with a viability of >80% and yield of >50% 10 following thawing. Many laboratories failed to reach this standard on the initial QAP 11 round. Interventions to improve performance included teleinterviews with the staff at 12 each laboratory, two annual wet workshops, and direct access to a senior scientist to 13 discuss performance following each QAP round. Performance improved substantially in 14 the majority of sites that initially failed the QAP (p = 0.002 and p = 0.001; viability and 15 yield, respectively). In a minority of laboratories there was no improvement (n=2) while a 16 high standard was retained at the laboratories that commenced with adequate 17 performance (n=3). These findings demonstrate that simple interventions and 18 monitoring of PBMC preparation and cryopreservation from multiple laboratories can 19 significantly improve performance and contribute to maintenance of a network of 20 laboratories accredited for quality PBMC fractionation and cryopreservation. 21 AC CE TE D Dyer, et al. Viability, yield and function QAP for cryopreserved PBMC 3
Magnetic resonance imaging of periventricular leukomalacia and its clinical correlation in children (1997)

Abstract The prevalence of periventricular leukomalacia and its association with clinical neurological signs in school‐age preterm children are unknown. We matched 42 eight‐year‐old children who were born before term with birth weights lower than 1,750 gm (mean, 1,410 gm; gestational age, 31 weeks) with 42 children who were born at term and of normal birth weight, to compare clinical neurological status and magnetic resonance imaging findings. Of the children born prematurely, 9.5% had cerebral palsy and 31% had minor neurological dysfunction whereas 9% of the children born at term had minor neurological dysfunction and none had cerebral palsy. Deviations in tongue movements, heel walking, Fogs test results, and finger opposition, as well as behavioral disturbances, differentiated the preterm from the full‐term group. The prevalence of periventricular leukomalacia among all children born prematurely was 32%. It was observed in all children with cerebral palsy, in 25% with minor neurological dysfunction, and in 25% of the clinically healthy preterm children. None of the children born at term had evidence of periventricular leukomalacia. Children with periventricular leukomalacia especially demonstrated poor performance on heel walking and Fogs test. Though commonly found in preterm children, periventricular leukomalacia is not uniformly associated with abnormal neurological findings. A through neurological examination is a better predictor of later developmental problems than is magnetic resonance imaging.
Triacylglycerol Fatty Acid Composition in Diet-Induced Weight Loss in Subjects with Abnormal Glucose Metabolism – the GENOBIN Study (2008)

ClinicalTrials.gov NCT00621205.
Cloning ofPseudomonas fluorescensCarboxylesterase Gene and Characterization of Its Product Expressed inEscherichia coli (1994)

A gene (estC) coding for an esterase (esterase III) of Pseudomonas fluorescens was cloned into Escherichia coli JM83. DNA sequencing showed a single open reading frame with GTG as a translation initiation codon for esterase III. This was confirmed by N-terminal amino acid sequence analysis of the purified esterase III protein from an E. coli clone. The promoter sequence and a potential Shine-Dalgarno sequence were followed by the coding sequence of the estC gene. The amino acid sequence deduced from the nucleotide sequence contains the consensus active site sequence, G-X-S-X-G, of serine esterase. The esterase III expressed in an E. coli clone was purified by ion-exchange chromatography and gel filtration. The native form of the enzyme was a monomer with a molecular weight of 41,000. The results of substrate specificity and the inhibitor studies suggest that this enzyme is a carboxylesterase (EC 3.1.1.1) and a serine residue is present at the active site of the enzyme.
Outer membrane vesicles derived from Klebsiella pneumoniae are a driving force for horizontal gene transfer (2021)

Abstract Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. Recent studies recognized these vesicles as vectors to horizontal gene transfer, however the parameters that mediate OMVs transfer within bacterial communities remain unclear. The present study highlights for the first time the transfer of plasmids containing resistance genes via OMVs derived from Klebsiella pneumoniae ( K. pneumoniae ). This mechanism confers DNA protection and it is plasmid copy number dependent with a ratio of 3.6 time among high copy-number plasmid (pGR) versus low copy number plasmid (PRM) and the transformation efficiency was 3.6 times greater. Therefore, the DNA amount in the vesicular lumen and the efficacy of horizontal gene transfer was strictly dependent on the identity of the plasmid. Moreover, the role of K. pneumoniae -OMVs in interspecies transfer was described. The transfer ability was not related to the phylogenetic characteristics between the donor and the recipient species. K. pneumoniae -OMVs transferred plasmid to Escherichia coli , Salmonella enterica , Pseudomonas aeruginosa and Burkholderia cepacia . These findings address the pivotal role of K. pneumoniae -OMVs as vectors for antimicrobial resistance genes spread, contributing to the development of antibiotic resistance in the microbial communities. Author summary K. pneumoniae is an important opportunistic pathogen that affects several host districts, in particular respiratory and urinary tracts. Hospital-acquired K. pneumoniae infections lead to a 50% mortality rate correlated with rapid acquisition of antibiotic resistance. Currently, the increasing rate of antibiotic resistance among K. pneumoniae isolates is a major concern worldwide. The spread of multidrug-resistant K. pneumoniae strains renders current therapeutic options ineffective. Like all Gram-negative bacteria, K. pneumoniae secretes OMVs. OMVs are spherical structures, with a diameter between 50-250 nm, originating from the outer membrane. OMVs biogenesis allows bacteria to interact with the external environment, increasing bacterial survival under stressful conditions and regulating microbial interactions within bacterial communities. Few evidence recognized OMVs as vectors for horizontal gene transfer, contributing to the spread of resistance. In this scenario, the present study examines the potential role of K. pneumoniae -OMVs in inter- and intra-species diffusion of ß-lactam resistance.
Stochastic Hybrid Approximation for Uncertainty Management in Gas-Electric Systems (2021)

Gas-fired generators, with their ability to quickly ramp up and down their electricity production, play an important role in managing renewable energy variability. However, these changes in electricity production translate into variability in the consumption of natural gas, and propagation of uncertainty from the electric grid to the natural gas system. To ensure that both systems are operating safely, there is an increasing need for coordination and uncertainty management among the electricity and gas networks. A challenging aspect of this coordination is the consideration of natural gas dynamics, which play an important role in intra-day operation, but give rise to a set of non-linear and non-convex equations that are hard to optimize even in the deterministic case. Ideally, the problem is formulated as a stochastic problem but many conventional methods for stochastic optimization are numerically intractable because they either incorporate a large number of scenarios directly or require the underlying problem to be convex. To address these challenges, we propose using a Stochastic Hybrid Approximation algorithm to more efficiently solve these problems and investigate the efficacy of several different variants of this algorithm. Our case study demonstrates that the proposed technique is able to quickly obtain high quality solutions and outperforms existing benchmarks such as Generalized Benders Decomposition. We demonstrate that coordinated uncertainty management that accounts for the gas system can significantly reduce both electric and gas system load shed in stressed conditions.
Analysis of Receptor-Ligand Interactions (1995)

Extensive description and analysis of receptor-ligand interactions, including how the number of potential binding sites on a receptor and the affinity of each binding site for its ligand may be determined.
Development of an E-Health App for Lower Limb Postoperative Rehabilitation Based on Plantar Pressure Analysis (2018)

The traditional postoperative rehabilitation training mode of lower limbs is mostly confined to hospitals or nursing sites. With the increase of postoperative patients, the current shortage of medical resources is obviously not satisfactory, and the medical costs are high, thus it is difficult to apply widely. A new mobile phone application (app) based on plantar pressure analysis is developed to fulfill the requirements of remote postoperative rehabilitation. It is designed, implemented, tested, and used for pilot experiment in conjunction with the system design methodology of the waterfall model. Preliminary testing and a pilot experiment showed that the app has realized basic functions and can achieve patient rehabilitation out of hospitals. The development of the app can shorten the hospitalization time of patients, reduce medical costs, and make up for the current shortage of medical resources. In the future, more experiments will be done to verify the effectiveness of the app.