gebhart/patrepeval huggingface dataset:
Preparative synthesis of GDP- -L-fucose by recombinant enzymes from enterobacterial sources (2000)

The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides). The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose. Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively. The activities of the Gmd and WcaG were analyzed. The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified. The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses. The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity. The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase. With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+. The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.
Optical Microspherical Resonators for Biomedical Sensing (2011)

Optical resonators play an ubiquitous role in modern optics. A particular class of optical resonators is constituted by spherical dielectric structures, where optical rays are total internal reflected. Due to minimal reflection losses and to potentially very low material absorption, these guided modes, known as whispering gallery modes, can confer the resonator an exceptionally high quality factor Q, leading to high energy density, narrow resonant-wavelength lines and a lengthy cavity ringdown. These attractive characteristics make these miniaturized optical resonators especially suited as laser cavities and resonant filters, but also as very sensitive sensors. First, a brief analysis is presented of the characteristics of microspherical resonators, of their fabrication methods, and of the light coupling techniques. Then, we attempt to overview some of the recent advances in the development of microspherical biosensors, underlining a number of important applications in the biomedical field.
Multiuser projection receivers (1996)

A new multiuser receiver for synchronous code-division multiple-access (CDMA) systems with error control coding is proposed. The receiver achieves interference cancellation by projecting the undesired users onto the space spanned by the desired users' signal vectors. The detector calculates the least squares (LS) estimate of the interfering users data, that is used to yield an adjusted metric for maximum likelihood sequence estimation (MLSE) for the desired users' sequences. Simulation results indicate that close to optimal performance can be achieved when all but one of the users are projected using only a single user decoder for the desired user. Further, an adaptive receiver structure based on the recursive LS update is presented that is well-suited for DSP implementation due to it's computational efficiency.
VDL Mode 4/STDMA-a CNS data link (2002)

At its tenth Air Navigation Conference (1991), the ICAO endorsed the so-called Communication, Navigation, Surveillance/Air Traffic Management concept, CNS/ATM. This concept envisages the use of data link communications, satellite navigation and automatic dependent surveillance (ADS). The Swedish Civil Aviation Administration (SCAA) has been involved in the technical and operational development of an integrated CNS/ATM since 1990. The system concept is based on the use of GNSS and a time synchronised VHF data link (VDL Mode 4/STDMA). The system is intended to support all elements of CNS. This paper discusses the requirements for an integrated CNS system. It also gives an overview of the VDL mode 4/STDMA system as well as description of on-going trials and standardisation effort within ICAO.
Selective Treatment of Pancreatic Cancer Cells by Plasma-Activated Saline Solutions (2017)

Atmospheric discharge between a metal pin cathode and saline solution (SS) anode with different air gap lengths was considered for investigating plasma self-organization patterns at the liquid anode surface. We report the effect of plasma-activated SSs on human pancreas adenocarcinoma cancer cells line (BxPC-3) and human pancreatic duct epithelial normal cells line (H6c7). The presence of reactive oxygen species and reactive nitrogen species resulted in the anti-cancer properties of plasma-activated SS.
ChemInform Abstract: Applications of Peptide Synthetases in the Synthesis of Peptide Analogues (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Better estimation of small sobol' sensitivity indices (2013)

A new method for estimating Sobol' indices is proposed. The new method makes use of 3 independent input vectors rather than the usual 2. It attains much greater accuracy on problems where the target Sobol' index is small, even outperforming some oracles that adjust using the true but unknown mean of the function. The new estimator attains a better rate of convergence than the old one in a small effects limit. When the target Sobol' index is quite large, the oracles do better than the new method.
Reversible depression in theTcof thin Nb films due to enhanced hydrogen adsorption (1998)

The effect of large hydrogen concentrations (\ensuremath{\approx}30% atomic), on the superconducting transition temperature ${T}_{c}$ of Nb films is discussed. The hydrogen is incorporated in solutionlike phases which occur at these concentrations because the (\ensuremath{\alpha} to \ensuremath{\beta}) hydride transition in these films is suppressed. X-ray-diffraction data show an asymmetric expansion of the [110] Nb interplanar spacing. This can be as high as a 6--8 % expansion perpendicular to the plane of the film for the addition of \ensuremath{\sim}70% hydrogen, with only a 1--2 % change in the plane of the film. ${T}_{c}$ is depressed to a value near 50% of that of the undoped film with about 30% atomic hydrogen, and returns to its initial value when the hydrogen is removed. A discussion is given of how both disorder and changes in the electronic structure can affect ${T}_{c}.$
Weighted electrode configuration for electromechanical coupling enhancement in a new class of micromachined Lithium Niobate laterally vibrating resonators (2012)

This paper reports on an electromechanical coupling (k <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) enhancement technique for a new class of Lithium Niobate based laterally vibrating MEMS resonators. This technique uses a weighted electrode configuration to experimentally demonstrate a more than 2X enhancement of the device k <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The resonator orientated 30° to +Y in the X-cut plane achieved a k <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> of 21.7%, and a quality factor of 1300, effectively corresponding to a figure of merit of 280 - the highest demonstrated to date for laterally vibrating resonators.
Supplementary Figure from Genome-Protective Topoisomerase 2a-Dependent G<sub>2</sub> Arrest Requires p53 in hTERT-Positive Cancer Cells (2023)

Supplementary Figure from Genome-Protective Topoisomerase 2a-Dependent G&lt;sub&gt;2&lt;/sub&gt; Arrest Requires p53 in hTERT-Positive Cancer Cells
Genetic Diversity Among Banana streak virus Isolates from Australia (2000)

ABSTRACT Banana streak virus (BSV) is an important pathogen of bananas and plantains (Musa spp.) throughout the world. We have cloned and sequenced part of the genomes of four isolates of BSV from Australia, designated BSV-RD, BSV-Cav, BSV-Mys, and BSV-GF. These isolates originated from banana cvs. Red Dacca, Williams, Mysore, and Goldfinger, respectively. All clones contained a sequence covering part of open reading frame III and the intergenic region of the badnavirus genome. The sequences were compared with those of other badnaviruses, including BSV-Onne, a previously characterized isolate from Nigeria. The BSV-RD sequence was virtually identical to that of BSV-Onne, differing by only two nucleotides over 1,292 bp. However, BSV-Cav, -Mys, and -GF were divergent in nucleotide sequence. Phylogenetic analyses using conserved sequences in the ribonuclease H domain revealed that all BSV isolates were more closely related to each other than to any other badnavirus. BSV-Cav was most closely related to BSV-Onne, and there was 95.1% identity between the two amino acid sequences. Other relationships between the BSV isolates were less similar, with sequence identities ranging from 66.4 to 78.2%, which is a magnitude comparable to the distance between some of the recognized badnavirus species. Immunocapture-polymerase chain reaction assays have been developed, allowing specific detection and differentiation of the four isolates of BSV.
Naturalistic Decision-making Based Collaboration Scenarios (2017)

This chapter discusses the development and use of naturalistic decision-making-based collaboration scenarios for better understanding the macrocognitive processes of team collaboration. By better understanding the processes of team collaboration through empirical research more effective team collaboration tools can be developed. The cornerstone of Naturalistic Decision-Making research is the Cognitive Task Analysis (CTA). The CTA provides a procedural methodology for understanding the cognitive skills used by experts performing a given task. Event-based scenarios are used extensively throughout the fields of business, education and research. In collaboration research, four types of scenarios are commonly used: gaming or pictorial scenarios, one dimensional scenarios, multidimensional scenarios, and multidimensional or multidomain scenarios. The complexity of multidimensional or multidomain scenarios ensures a sense of realism and operational relevance as it applies across integrated domains. It requires situational constraints, for example time pressure, information uncertainty, dynamic information, cognitive overload, to ensure the realism critical to content validity.
Respiratory Inductance Plethysmography is suitable for voluntary hyperventilation test (2007)

The aim of this work was to evaluate the goodness of fit of a signal issued of the respiratory inductance plethysmography (RIP) derivative to the airflow signal during rest, voluntary hyperventilation, and recovery. RIP derivative signal was filtered with an adjusted filter based on each subject airflow signal (pneumotachography). For each subject and for each condition (rest, voluntary hyperventilation, and recovery) comparisons were performed between the airflow signal and the RIP derivative signal filtered with an adjusted filter obtained either on rest signal or on the studied part of the signals (voluntary hyperventilation or recovery). Results show that the goodness of fit was : (1) higher than 90% at almost all comparisons (122 on 132), (2) not improved by applying an adjusted filter obtained on the studied part of the signals. These results suggest that RIP could be used for studying breathing during voluntary hyperventilation and recovery using adjusted filters obtained from comparison to airflow signal at rest.
Participatory business process reengineering design: generating solutions (2002)

PAWS (Participatory Approach to Workgroup Systems) is an alternative approach to radical BPR (business process reengineering). Its main feature is to include participatory design by employees in the reengineering activity. It consists of a set of sequential stages. The third of these stages is intended to obtain the largest possible number of alternative solutions to the previously identified problems of the organization during the second stage of the PAWS method. Since the idea is to make employees participate, the consultant must be able to motivate workers to generate ideas. It will probably take several rounds of participatory work and individual sessions to achieve this generation. Mechanisms to motivate participation may be designed; many of them can be supported by a computer system. Three aspects of the work related to PAWS Stage 3 are presented. The first is a participatory model, making explicit the elements which motivate people to participate in a collaborative work environment. The second is the computer mechanisms designed to motivate participation. Finally, the third is a software prototype based on the proposed GPS (Generation oPtions Support) participatory model.
Conductivity control of Sn-doped α-Ga2O3 thin films grown on sapphire substrates (2016)

Abstract We achieved the successful fabrication of Sn-doped α-Ga 2 O 3 thin films with higher electron mobility and wider conductivity controls by improving the crystal quality. α-Ga 2 O 3 films showed n-type conductivity with a maximum electron mobility of 24 cm 2 V −1 s −1 . The carrier concentration was successfully controlled in the range of 10 17 –10 19 cm −3 . Crystal defects such as dislocations severely compensate the free carriers in α-Ga 2 O 3 films and restrict the mobility at low carrier concentrations. Therefore, to achieve further conductivity control and higher mobility, improving the crystallinity of α-Ga 2 O 3 films is necessary.
SYSTEMIC CELLULAR HYPERSENSITIVITY INDUCED BY AN INTESTINALLY ABSORBED ANTIGEN (1974)

Neonatal mice given intact living Schistosoma mansoni eggs or soluble schistosome egg antigens intragastrically developed systemic cellular hypersensitivity as shown by markedly accelerated, augmented granulomatous inflammation around S. mansoni eggs subsequently injected intravenously into the pulmonary microvasculature. To achieve partial sensitization in adult mice schistosome eggs had to be administered intragastrically with bicarbonate; full sensitization occurred when the eggs were injected intraduodenally. These data indicate that under appropriate conditions intestinal administration of antigen can result in systemic cellular immune sensitization.
Effect of the Oxygen Sub-Stoichiometry and of Hydrogen Insertion on the Formation of Intermediate Bands within the Gap of Disordered Molybdenum Oxide Films (2013)

The electronic structure of disordered-amorphous molybdenum oxide films was investigated near the band gap by optical absorption and photoluminescence (PL) measurements. It was found that, in nearly stoichiometric films, the 3.2 eV wide gap is free of states and a PL band at 3.7 eV was attributed to electronic transitions between the Mo 4d bonding–antibonding orbitals. In sub-stoichiometric films, electronic states appear within the gap giving rise to additional PL emission within the range 3–3.25 eV. The band gap remains at 3.2 eV, and intermediate bands (IB) are formed within the gap for oxygen-deficient hydrogenated samples. A substantial increase of the PL intensity within the range 2.75–3.75 eV was observed, attributed to the IBs and the increase of the density of Mo 4d antibonding states within the conduction band (CB). The band gap decreases to 2.7 eV in sub-stoichiometric and hydrogenated samples, while the rest of the elctronic structure remains unchanged as for hydrogenated samples also giving enhanced PL intensity within the same range.
Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Hydroxytriazines (1997)

Hydroxytriazines are environmental decomposition products of s-triazine herbicides. Enzyme-linked immunosorbent assays (ELISAs) in various formats for the detection of hydroxypropazine, hydroxyatrazine, and hydroxysimazine were developed. 6-{4-Methoxy-6-[(1-methylethyl)amino]-1,3,5-triazin-2-yl}aminohexanoic acid was used as the immunizing hapten. One of the resulting ELISAs has IC50 values of 0.002, 0.004, and 0.017 μM (0.4, 0.8, and 3.1 ppb) for hydroxypropazine, hydroxyatrazine, and hydroxysimazine, respectively. This ELISA utilizes a coated antigen format with a simazine−mercaptopropanoic acid hapten conjugated to conalbumin as the coating antigen. The assay parameters are unchanged at pH values between 5.0 and 8.0 and at ionic strengths from 0.13 to 1.0 M phosphate-buffered saline. Methanol does not affect the assay up to 13%, whereas acetonitrile and acetone cause a decrease of sensitivity at 3.1%. Groundwater fortified with hydroxyatrazine was analyzed according to this method. Good correlations with spike levels were observed, making this assay potentially valuable for the screening of groundwater samples containing parts per trillion levels of hydroxytriazine contamination. Keywords: s-Triazine; hydroxytriazine; hydroxyatrazine; hydroxysimazine; hydroxypropazine; ELISA; enzyme immunoassay; herbicide metabolites
Tensile Properties and Microstructure of Inconel 718 Fabricated with Electron Beam Freeform Fabrication (EBF(sup 3)) (2009)

Electron beam freeform fabrication (EBF3) direct metal deposition processing was used to fabricate two Inconel 718 single-bead-width wall builds and one multiple-bead-width block build. Specimens were machined to evaluate microstructure and room temperature tensile properties. The tensile strength and yield strength of the as-deposited material from the wall and block builds were greater than those for conventional Inconel 718 castings but were less than those for conventional cold-rolled sheet. Ductility levels for the EBF3 material were similar to those for conventionally-processed sheet and castings. An unexpected result was that the modulus of the EBF3-deposited Inconel 718 was significantly lower than that of the conventional material. This low modulus may be associated with a preferred crystallographic orientation resultant from the deposition and rapid solidification process. A heat treatment with a high solution treatment temperature resulted in a recrystallized microstructure and an increased modulus. However, the modulus was not increased to the level that is expected for Inconel 718.
The Status, Trends and Role of International CSP Research and Development (2015)

DLR Research Areas Energy, Renewables and Solar Power Concentrating Solar Power (CSP) The Costs of Electricity from RE The Markets of CSP The Role of CSP for China
Integration of DNA Methylation, Copy Number Variation, and Gene Expression for Gene Regulatory Network Inference and Application to Psychiatric Disorders (2014)

Biological network inference is a crucial problem to solve in Bioinformatics as most of biological process are based on bio molecular interactions. Many researchers have worked on especially the inference of gene regulatory networks where a node and edge represent a gene and regulation relationship respectively assuming that a gene can regulate another gene indirectly. However, a gene expression level can be influenced by not only genes and proteins but also other biological factors. Therefore, the inference could be more effective if those factors are considered in gene regulatory network inferences. In this paper, we propose an integrative approach to infer gene regulatory networks where a gene can be regulated by not only gene and but also DNA Methylation and copy number variation. It is assumed that a gene can be directly regulated by a single DNA Methylation and copy number variation at most. The simulation results show that our method outperforms popular and state-of-the-art methods of biological network inference. In addition, we applied the proposed method to psychiatric disorder data. The inferred networks provide the relationships within a set of genes that are more likely to be regulated by DNA Methylation and copy number variation of the genes.
Evaluation of honeybeesʼ products from lake Prespa region-Greece (2022)

Due to consumers' growing interest for natural and healthy foods, in connection with our continuous studies on bee-keeping products [1], [2], we present in this study the pollen profile and chemical analyses of 11 honey samples, 9 propolis and 3 royal jellies (RJ) from the lake Prespa region, further evaluated for their bioactivities.
Results After Partial Left Ventriculectomy in a European Heart Failure Population (1999)

Patients with end-stage idiopathic or ischemic cardiomyopathies can be improved considerably with partial left ventriculectomy. Any cardiac comorbidity should be repaired simultaneously.
Defective Chemotaxis and Calcium Response in Localized Juvenile Periodontitis Neutrophils (1993)

Localized juvenile periodontitis (ljp) is an early onset form of periodontal disease characterized by unique localization to first molars and incisors and a high prevalence of neutrophil abnormalities, particularly chemotaxis. The intracellular transduction mechanisms that follow receptor-ligand coupling on the neutrophil surface and lead to chemotaxis are not clearly established. Chemotaxis and phagocytosis are modulated by a variety of receptors and involve several activation pathways; the role of intracellular calcium as a presumptive second messenger and mediator of these events is well established. The putative effector mechanisms for the chemotactic receptor of neutrophils also include the possible activation of a phospholipase, protein kinase C, methyltransferase, or adenylate cyclase. In normal neutrophils, a phosphoinositide pathway initiated by phospholipase C, which results in the activation of protein kinase C via diacylglycerol and the generation of IP3, has been implicated. In order to better understand the stages of neutrophil transduction, fluorescent probes were used to monitor neutrophil calcium changes. Chlorotetracycline (CTC) was used as an indirect probe of intracellular membrane-bound pool of calcium stores, and Quin-2 was used to monitor cytosolic free calcium levels of FMLP stimulated normal and LJP neutrophils. The results indicate that the early phase of the calcium response affiliated with the release of intracellularly sequestered calcium appears intact in LJP neutrophils, as the CTC fluorescence changes were similar to control values. The second phase of the calcium response, associated with membrane channel activation and an influx of extracellular calcium, appeared compromised in the neutrophils of the LJP population.(ABSTRACT TRUNCATED AT 250 WORDS)