gebhart/patrepeval huggingface dataset:
Mitochondrial damage revealed by immunoselection for ALS-linked misfolded SOD1 (2013)

Mutant superoxide dismutase 1 (SOD1) selectively associates with spinal cord mitochondria in rodent models of SOD1-mediated amyotrophic lateral sclerosis. A portion of mutant SOD1 exists in a non-native/misfolded conformation that is selectively recognized by conformational antibodies. Misfolded SOD1 is common to all mutant SOD1 models, is uniquely found in areas affected by the disease and is considered to mediate toxicity. We report that misfolded SOD1 recognized by the antibody B8H10 is present in greater abundance in mitochondrial fractions of SOD1(G93A) rat spinal cords compared with oxidized SOD1, as recognized by the C4F6 antibody. Using a novel flow cytometric assay, we detect an age-dependent deposition of B8H10-reactive SOD1 on spinal cord mitochondria from both SOD1(G93A) rats and SOD1(G37R) mice. Mitochondrial damage, including increased mitochondrial volume, excess superoxide production and increased exposure of the toxic BH3 domain of Bcl-2, tracks positively with the presence of misfolded SOD1. Lastly, B8H10 reactive misfolded SOD1 is present in the lysates and mitochondrial fractions of lymphoblasts derived from ALS patients carrying SOD1 mutations, but not in controls. Together, these results highlight misfolded SOD1 as common to two ALS rodent animal models and familial ALS patient lymphoblasts with four different SOD1 mutations. Studies in the animal models point to a role for misfolded SOD1 in mitochondrial dysfunction in ALS pathogenesis.
Treating phenolic pollutants with membranes (2001)

Pervaporation and extractive membrane sepn. were studied to remove phenolic pollutants from wastewater. [on SciFinder (R)]
Dlk1 regulates quiescence in calcitonin receptor-mutant muscle stem cells (2020)

Muscle stem cells, also called muscle satellite cells (MuSCs), are responsible for skeletal muscle regeneration and are sustained in an undifferentiated and quiescent state under steady conditions. The calcitonin receptor (CalcR)-protein kinase A (PKA)-Yes-associated protein 1 (Yap1) axis is one pathway that maintains quiescence in MuSCs. Although CalcR signaling in MuSCs has been identified, the critical CalcR signaling targets are incompletely understood. Here, we show the relevance between the ectopic expression of delta-like non-canonical Notch ligand 1 (Dlk1) and the impaired quiescent state in CalcR-conditional knockout (cKO) MuSCs. Dlk1 expression was rarely detected in both quiescent and proliferating MuSCs in control mice, whereas Dlk1 expression was remarkably increased in CalcR-cKO MuSCs at both the mRNA and protein levels. It is noteworthy that all Ki67<sup>+</sup> non-quiescent CalcR-cKO MuSCs express Dlk1, and non-quiescent CalcR-cKO MuSCs are enriched in the Dlk1<sup>+</sup> fraction by cell sorting. Using mutant mice, we demonstrated that PKA-activation or Yap1-depletion suppressed Dlk1 expression in CalcR-cKO MuSCs, which suggests that the CalcR-PKA-Yap1 axis inhibits the expression of Dlk1 in quiescent MuSCs. Moreover, the loss of Dlk1 rescued the quiescent state in CalcR-cKO MuSCs, which indicates that the ectopic expression of Dlk1 disturbs quiescence in CalcR-cKO. Collectively, our results suggest that ectopically expressed Dlk1 is responsible for the impaired quiescence in CalcR-cKO MuSCs.
Short-term associations between ambient air pollution and acute atrial fibrillation episodes (2020)

These results suggest that in an urban setting with relatively low levels of ambient air pollution, hourly changes in pollutant levels may increase the risk of acute episodes of both asymptomatic and symptomatic AF, especially among people with diabetes, hypertension or overweight.
P02-147 - Changing Feelings: How the Serotonin Transporter Genotype Modulates Emotion Regulation (2010)

Major depression (MD) is one of the most common disease burdens throughout the world. Understanding the pathophysiology of MD is therefore a major challenge in psychology and psychiatry. One major risk factor for MD is stress. However, the risk of depression after stressful life events is much greater in individuals with possession of the short variant (S) compared to the long variant (L) of the serotonin transporter polymorphic region (5-HTTLPR). But why? One intriguing possibility is that 5-HTTLPR-S carriers show reduced cognitive downregulation of negative emotional information, through which negative emotional information is easily interpreted as stressful thereby increasing the risk of depression. In support of this hypothesis, a hyperresponsiveness (i.e. in 5-HTTLPR-S carriers compared to 5-HTTLPR-L carriers) of the amygdala in response to emotionally negative information has been found. Further, amygdala reactivity is negatively correlated with cognitive down regulation (cognitive reappraisal) of emotionally negative information. To explore the effect of cognitive reappraisal on neural and behavioral emotional responses, instructed emotion regulation (i.e. instructing participants to down-regulate or up-regulate the impact of negative emotional stimuli by reinterpreting the meaning of an emotional event) can be applied. Therefore, the main aim of the current study is to investigate whether the effects of cognitive reappraisal of negative emotional information (by instructed reappraisal) on neural and behavioral emotional responses is modulated by 5-HTTLPR genotype using functional magnetic resonance imaging (fMRI).
Serum Amyloid A Binding to CLA-1 (CD36 and LIMPII Analogous-1) Mediates Serum Amyloid A Protein-induced Activation of ERK1/2 and p38 Mitogen-activated Protein Kinases (2004)

Serum amyloid A protein (SAA) is an acute-phase reactant, known to mediate pro-inflammatory cellular responses. This study reports that CLA-1 (CD36 and LIMPII Analogous-1; human orthologue of the Scavenger Receptor Class B Type I (SR-BI)) mediates SAA uptake and downstream SAA signaling. Flow cytometry experiments revealed more than a 5-fold increase of Alexa-488 SAA uptake in HeLa cells stably transfected with CLA-1. Alexa 488-HDL uptake directly correlated with SAA uptake when determined in several CLA-1 stably transfected HeLa cell clones expressing various levels of CLA-1. SAA directly binds to CLA-1 as determined by cross-linking and colocalization of anti-CLA-1 antibody with SAA. SAA was co-internalized with transferrin to the endocytic recycling compartment pointing to a potential site of SAA metabolism. Alexa-488 SAA uptake in the CLA-1-overexpressing HeLa cells, as well as in THP-1 monocyte cell line, can be efficiently blocked by unlabeled SAA, high density lipoprotein, and other CLA-1 ligands. At the same time, markedly enhanced levels of phosphorylation of the mitogen-activated protein kinases (MAPKs), ERK1/2, and p38, were observed in cells stably transfected with CLA-1 cells following SAA stimulation when compared with mock transfected cells. The levels of the SAA-induced interleukin-8 (IL-8) secretion by CLA-1-overexpressing cells also significantly exceeded (5- to 10-fold) those detected for control cells. Synthetic amphipathic peptides possessing a structural alpha-helical motif inhibited SAA-induced activation of both MAPKs and IL-8 secretion in THP-1 cells. The results of this study demonstrate for the first time that CLA-1 functions as an endocytic SAA receptor and is involved in SAA-mediated cell signaling events associated with the immune-related and inflammatory effects of SAA.
Chronic Zinc Deficiency Alters Chick Gut Microbiota Composition and Function (2015)

Zinc (Zn) deficiency is a prevalent micronutrient insufficiency. Although the gut is a vital organ for Zn utilization, and Zn deficiency is associated with impaired intestinal permeability and a global decrease in gastrointestinal health, alterations in the gut microbial ecology of the host under conditions of Zn deficiency have yet to be studied. Using the broiler chicken (Gallus gallus) model, the aim of this study was to characterize distinct cecal microbiota shifts induced by chronic dietary Zn depletion. We demonstrate that Zn deficiency induces significant taxonomic alterations and decreases overall species richness and diversity, establishing a microbial profile resembling that of various other pathological states. Through metagenomic analysis, we show that predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways responsible for macro- and micronutrient uptake are significantly depleted under Zn deficiency; along with concomitant decreases in beneficial short chain fatty acids, such depletions may further preclude optimal host Zn availability. We also identify several candidate microbes that may play a significant role in modulating the bioavailability and utilization of dietary Zn during prolonged deficiency. Our results are the first to characterize a unique and dysbiotic cecal microbiota during Zn deficiency, and provide evidence for such microbial perturbations as potential effectors of the Zn deficient phenotype.
Comparative performance of five commercially available serologic assays to detect antibodies to SARS-CoV-2 and identify individuals with high neutralizing titers (2020)

Accurate serological assays to detect antibodies to SARS-CoV-2 are needed to characterize the epidemiology of SARS-CoV-2 infection and identify potential candidates for COVID-19 convalescent plasma (CCP) donation. This study compared the performance of commercial enzyme immunoassays (EIAs) to detect IgG or total antibodies to SARS-CoV-2 and neutralizing antibodies (nAb). The diagnostic accuracy of five commercially available EIAs (Abbott, Euroimmun, EDI, ImmunoDiagnostics, and Roche) to detect IgG or total antibodies to SARS-CoV-2 was evaluated from cross-sectional samples of potential CCP donors that had prior molecular confirmation of SARS-CoV-2 infection for sensitivity (n=214) and pre-pandemic emergency department patients for specificity (n=1,102). Of the 214 potential CCP donors, all were sampled >14 days since symptom onset and only a minority had been hospitalized due to COVID-19 (n=16 [7.5%]); 140 potential CCP donors were tested by all five EIAs and a microneutralization assay. When performed according to the manufacturers' protocol to detect IgG or total antibodies to SARS-CoV-2, the sensitivity of each EIA ranged from 76.4% to 93.9%, and the specificity of each EIA ranged from 87.0% to 99.6%. Using a nAb titer cutoff of ≥160 as the reference positive test (n=140 CCP donors), the empirical area under receiver operating curve of each EIA ranged from 0.66 (Roche) to 0.90 (Euroimmun). Commercial EIAs with high diagnostic accuracy to detect SARS-CoV-2 antibodies did not necessarily have high diagnostic accuracy to detect high nAbs. Some but not all commercial EIAs may be useful in the identification of individuals with high nAbs in convalescent individuals.
Development of a bacterial bioassay for atrazine and cyanuric acid detection (2015)

The s-triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative method for physicochemical quantification using two bioluminescent bacterial biosensors: E. coli SM003 for cyanuric acid detection and E. coli SM004 for both atrazine and cyanuric acid detection. The concentration of cyanuric acid detection for E. coli SM003 ranges from 7.83 μM to 2.89 mM, and for E. coli SM004 ranges from 0.22 to 15 μM. Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM. According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water. Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.
Relationship between airway inflammation and the frequency of exacerbations in patients with smoking related COPD (2001)

There are several clinical features that directly influence bronchial inflammation in COPD. When these were carefully controlled for, patients with more frequent reported exacerbations had lower sputum concentrations of SLPI. This important antiproteinase is also known to possess antibacterial and antiviral activity. Further studies are required into the nature of recurrent exacerbations and, in particular, the regulation and role of SLPI in affected individuals.
Principal component filter banks for optimum wavelet analysis (2003)

Optimality issues are addressed when multiresolution methods are applied to random signals. An optimal P-band perfect reconstruction filter bank is derived, which decomposes the input signal into its lower-resolution principal components. Results are developed for stationary processes and a class of nonstationary processes, which includes cyclostationary signals. FIR designs are discussed and some examples presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Anti-CD3 monoclonal antibody-mediated cytotoxicity occurs through an interleukin-2-independent pathway in CD3+ large granular lymphocytes (1990)

The mechanism of induction of cytotoxicity produced by anti-CD3 monoclonal antibody (MoAb) was studied in four patients with CD3+ large granular lymphocyte (LGL) leukemia. Anti-CD3 MoAb treatment resulted in increased target cell binding and increased granule formation. After activation, leukemic LGL remained Tac-, with the exception of a patient with CD4+ LGL leukemia. Radiolabeled interleukin-2 (IL-2) binding studies demonstrated that treatment with anti-CD3 MoAb resulted in upregulation of the number of p75 intermediate affinity IL-2 receptor sites per cell. Northern blot hybridization analysis showed expression of gamma-interferon gene transcripts 24 to 48 hours after activation. There was no evidence for expression of IL-2 messenger RNA or secretion of IL-2 after activation. Anti-CD3 MoAb and IL-2 provide different signals for activation of CD3+ LGL. Induction of cytotoxicity produced by anti-CD3 MoAb in leukemic CD3+ LGL is not associated with IL-2 production.
The prevalence of primary dystonia in the general community (2002)

The prevalence of primary dystonia was assessed in a random population sample of individuals aged 50 and over in Bruneck, South Tyrol. The diagnosis of primary dystonia was confirmed by videotaped review. Primary dystonia was present in 6 of 707 cases resulting in a prevalence rate of 732 per 100,000 (95% CI 319-1,564) in the general population aged 50 and over. Only two cases (33%) had been previously diagnosed. These results indicate that the true prevalence of primary dystonia is significantly higher than published rates.
A phase II trial of selumetinib in children with recurrent optic pathway and hypothalamic low-grade glioma without NF1: a Pediatric Brain Tumor Consortium study (2021)

Selumetinib was tolerable and led to responses and prolonged disease stability in children with recurrent/progressive OPHGs based upon radiographic response, PFS, and visual outcomes.
TripPlanner: Personalized Trip Planning Leveraging Heterogeneous Crowdsourced Digital Footprints (2014)

Planning an itinerary before traveling to a city is one of the most important travel preparation activities. In this paper, we propose a novel framework called TripPlanner, leveraging a combination of location-based social network (i.e., LBSN) and taxi GPS digital footprints to achieve personalized, interactive, and traffic-aware trip planning. First, we construct a dynamic point-of-interest network model by extracting relevant information from crowdsourced LBSN and taxi GPS traces. Then, we propose a two-phase approach for personalized trip planning. In the route search phase, TripPlanner works interactively with users to generate candidate routes with specified venues. In the route augmentation phase, TripPlanner applies heuristic algorithms to add user's preferred venues iteratively to the candidate routes, with the objective of maximizing the route score while satisfying both the venue visiting time and total travel time constraints. To validate the efficiency and effectiveness of the proposed approach, extensive empirical studies were performed on two real-world data sets from the city of San Francisco, which contain more than 391 900 passenger delivery trips generated by 536 taxis in a month and 110 214 check-ins left by 15 680 Foursquare users in six months.
Maximizing the Potency of siRNA Lipid Nanoparticles for Hepatic Gene Silencing In Vivo** (2012)

Der Einfluss des pKS-Werts von ionisierbaren Lipiden auf den siRNA-Transport durch Lipidnanopartikel in vivo wurde anhand zahlreicher Modifikationen der Lipid-Kopfgruppen studiert. Dabei wurde ein Zusammenhang zwischen pKS-Wert und Stummschaltung des Maus-FVII-Gens (FVII ED50) gefunden: pKS-Werte von 6.2–6.5 erwiesen sich als optimal (siehe Diagramm). Für das wirksamste kationische Lipid betrug ED50 etwa 0.005 mg kg−1 in Mäusen und <0.03 mg kg−1 in Primaten (außer Menschen).
Time-decentralized DOA estimation for electronic surveillance (2017)

Due to high data rates necessary in multi-channel Electronic Surveillance systems, the full multi-channel data collected on each radar pulse is data compressed to a pulse descriptor word (PDW), which conventionally contains a DOA estimate for the pulse. It is only in down stream processing that the PDWs are clustered into groups identified as originating from single radar. A combined DOA estimate for the radar is conventionally achieved by some form of averaging over the individual pulse DOAs. If one could retain all of the multi-channel IQ data for all of the pulses in a cluster, one could optimally estimate a DOA for the radar using, for example, a maximum likelihood (ML) estimate. In this paper we propose a modification to the initial data compression and subsequent processing which, while adding only a modest amount of data to each PDW, allows estimation of the radar's DOA with performance approaching that which could be achieved by ML estimation using the full multi-channel data records for all the pulses in the cluster. The method is computationally efficient and also allows the optimal beamforming of each pulse without the need to explicitly estimate its DOA.
The fitness analysis of batch sedimentation test data (2010)

The batch settling test is an important method for the sizing of the industry continuous thickeners and the data analysis of the testing is based on the Wilhelm and Naide's theory, by which the Unit Area can be decided with an approximate expression of settling velocity and solids concentration. The velocities corresponding to the different solid concentrations can be determined through the graphical analysis method or numerical method which can give more precision solution. To handle the test data with numerical method, the expression of the settling curve (the interface height versus the settling time) and expression of the settling velocity curve (settling velocity versus solids concentration) should be selected. In this paper, both of single exponential expressions and double exponential expressions are investigated for the settling curve and single exponential expression, power expression, double exponential expression and rational expression are investigated for the settling velocity curve. The studies showed that the double exponential expression obtained more precision solution in the most situations.
Circulating EM66 is a highly sensitive marker for the diagnosis and follow‐up of pheochromocytoma (2005)

We have previously demonstrated that measurement of tissue concentration of the novel secretogranin II-derived peptide EM66 may help to discriminate between benign and malignant pheochromocytomas. The aim of the present study was to characterize EM66 in plasma and urine of healthy volunteers and pheochromocytoma patients, in order to further evaluate the usefulness of this peptide as a circulating marker for the management of the tumors. HPLC analysis of plasma and urine samples demonstrated that the EM66-immunoreactive material coeluted with the recombinant peptide. In healthy volunteers, plasma and urinary EM66 levels were, respectively, 2.6 (1.9-3.7) ng/ml and 2.9 (1.9-4.6) ng/ml. In patients with pheochromocytoma, plasma EM66 levels were 10-fold higher than those of healthy volunteers (26.9 (7.3-44) ng/ml), and returned to normal values after removal of the tumor. In contrast, urinary EM66 levels were not significantly different from those of healthy volunteers (3.2 (2.2-3.9) ng/ml). Measurement of total or free plasma metanephrines and 24 hr urinary metanephrines in our series of patients revealed that these tests, taken separately, are less sensitive than the EM66 determination. Pheochromocytes in primary culture secreted high levels of EM66, suggesting that the chromaffin tumor was actually responsible for the increased plasma peptide concentrations in the patients. These data indicate that EM66 is secreted in the general circulation and that elevated plasma EM66 levels are correlated with the occurrence of pheochromocytoma. Thus, EM66 is a sensitive plasma marker that should be considered as a complementary tool in the management of pheochromocytoma.
Protein-Free Hapten-Carbon Nanotube Constructs Induce the Secondary Immune Response (2017)

Carbon nanotubes are novel technological tools with multiple applications. The interaction between such nanoparticles and living organisms is nowadays a matter of keen research by academic and private institutions. In this study, carbon nanotube constructs were investigated as delivery vehicles for immunostimulation and induction of the secondary immune response to a small organic molecule, namely, a hapten. Two types of nanoconstructs were prepared: on one hand, carbon nanotubes carrying a protein bioconjugate of a hapten covalently linked to the carbon surface, and on the other hand, covalent carbon nanotube constructs of the same model chemical compound without the carrier protein. Nanotube vehicles carrying a hapten-protein bioconjugate were demonstrated to stimulate the immune system and to induce a strong primary immune response against the hapten with as low as 0.1 μg of the model chemical. The influence of the different elements of those nanoconstructs over the immune response was investigated to better understand the molecular mechanisms that are involved. As expected, the presence of the carrier protein was shown to be necessary in order to trigger the immune response. Interestingly, we found that a remarkable secondary immune response to the model organic compound occurred in the absence of a carrier protein. Additionally, a satisfactory adjuvant effect of carbon nanotubes was observed and a potent immune response was elicited without employing an oil-based adjuvant.
Ontological Descriptions for Integrating Design Information of Product-Service Systems (2020)

Product-service systems (PSS), which create value by integrating physical products and services, have received much attention as a promising option to increase manufacturers’ revenue and reduce environmental impact. The process of designing a PSS requires collaboration among various experts who use domain-specific knowledge. Therefore, several researches have been investigated for developing design tools tailored to their expertise. However, while the specialization of design tools can be useful for experts, it hinders companies from ensuring the integrity of design information in different design elements. This results in the failure in achieving expected benefits. To address these issues, this study applies the concept of interoperability to PSS design to integrate design information from different domains. In particular, ontological descriptions is adopted to achieve semantic interoperability in different design elements. The application of the proposed ontology to a lecture on PSS design highlights that the proposed method is effective for integrating information on PSS design elements and those between value creation and capture.