gebhart/patrepeval huggingface dataset:
Human liver-derived CXCR6+ NK cells are predominantly educated through NKG2A and show reduced cytokine production (2019)

NK cells have been implicated to affect the outcome of numerous liver diseases. In particular, members of the killer-cell Ig-like receptor (KIR) family, predominantly expressed by NK cells, have been associated with the outcome of hepatitis C virus infection and clearance of hepatocellular carcinoma. Inhibitory KIRs tune NK cell function through interaction with HLA class I, a process termed education. Nevertheless, the impact of the hepatic environment on NK cell education is incompletely understood. Therefore, we investigated the composition and function of hepatic KIR-expressing NK cells. Matched PBMC and hepatic lymphocytes were isolated from 20 individuals undergoing liver surgery and subsequently phenotypically analyzed for expression of KIRs and markers for tissue residency using flow cytometry. NK cell function was determined by co-culturing NK cells with the target cell line 721.221 and subsequent assessment of CD107a, IFN-γ, and TNF-α expression. Liver-resident CXCR6<sup>+</sup> /CD56<sup>Bright</sup> NK cells lacked KIRs and were predominantly educated through NKG2A, while CXCR6<sup>-</sup> /CD16<sup>+</sup> NK cells expressed KIRs and resembled peripheral blood NK cells. Hepatic NK cells showed lower response rates compared to peripheral blood NK cells; in particular, CXCR6<sup>+</sup> NK cells were hyporesponsive to stimulation with target cells. The high proportion of educated NK cells in both subsets indicates the importance of self-inhibitory receptors for the balance between maintenance of self-tolerance and functional readiness. However, the reduced functionality of hepatic NK cells may reflect the impact of the tolerogenic hepatic environment on NK cells irrespective of NK cell education.
Security Management of Next Generation Telecommunications Networks and Services (2013)

This book will cover network management security issues and currently available security mechanisms by discussing how network architectures have evolved into the contemporary NGNs which support converged services (voice, video, TV, interactive information exchange, and classic data communications). It will also analyze existing security standards and their applicability to securing network management. This book will review 21st century security concepts of authentication, authorization, confidentiality, integrity, nonrepudiation, vulnerabilities, threats, risks, and effective approaches to enc
Replication data for: Magnetically responsive peptide coacervates for dual hyperthermia and chemotherapy treatments of liver cancer (2020)

Liver cancer is an aggressive malignancy associated with high levels of mortality and morbidity. Doxorubicin (Dox) is often used to slow down liver cancer progression; however its efficacy is limited, and its severe side effects prevent its routine use at therapeutic concentrations. We present a biomimetic peptide that coacervates into micro-droplets, within which both Dox and magnetic nanoparticles (MNPs) can be sequestered. These Dox-loaded Magnetic Coacervates (DMCs) can be used for thermo-chemotherapy, with the controlled release of Dox triggered by an external Alternating Magnetic Field (AMF). The DMCs are internalized by the cells via an energy-independent mechanism which is not based on endocytosis. Application of AMF generates a temperature of 45 °C within the DMCs, triggering their disassembly and the simultaneous release of Dox, thereby resulting in dual hyperthermia and chemotherapy for more efficient cancer therapy. In vitro studies conducted under AMF reveal that DMCs are cytocompatible and effective in inducing HepG2 liver cancer cell death. Thermo-chemotherapy treatment against HepG2 cells is also shown to be more effective compared to either hyperthermia or chemotherapy treatments alone. Thus, our novel peptide DMCs can open avenues in theranostic strategies against liver cancer through programmable, wireless, and remote control of Dox release.
ChemInform Abstract: Microwave Spectral Study of 3‐Chloropropionitrile and 3‐ Bromopropionitrile. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The plant‐derived alkaloid aloperine prevents ischemia/reperfusion injury‐induced sudden cardiac death (2023)

Sudden cardiac death (SCD) remains a major cause of global mortality. In addition to modern interventions, botanical folk medicines have long been used to treat cardiovascular disease, although the efficacy and underlying mechanisms are often unresolved. Aloperine, a bioactive quinolizidine alkaloid isolated from Sophora alopecuroides plants, exhibits antioxidant, anti-inflammatory, antitumor, and vasorelaxant properties, but possible antiarrhythmic effects of aloperine in SCD are unclear. Here, we examined whether aloperine protects against ischemia and reperfusion injury-associated lethal ventricular arrhythmia and sudden cardiac death. Rats were divided into sham, control, and aloperine groups, and reperfusion-provoked ventricular arrhythmogenesis, cardiac damage markers, and signaling pathways quantified following left main coronary artery ischemia and reperfusion. In vitro studies of effects of aloperine on hERG and Kv4.3 cardiac voltage-gated potassium (Kv) channels were performed using two-electrode voltage clamp analysis of cloned channels expressed in Xenopus laevis oocytes. Aloperine pretreatment (10 mg/kg) did not affect baseline cardiac electrical stability; yet, it reduced ventricular arrhythmogenesis and susceptibility to SCD (mortality rate: control: 64.3%; aloperine: 0%) induced by reperfusion injury. Aloperine also reduced serum levels of LDH, CK-MB, α-HBDH, and cTnI post-I/R, and stimulated phosphorylation of ventricular ERK1/2 and STAT-3, which are key components of RISK and SAFE signaling pathways. Inhibition of either ERK1/2 (with U0126) or STAT-3 (with Ag490) abolished aloperine-induced anti-arrhythmic effects and ERK1/2 and STAT-3 phosphorylation. Interestingly, while aloperine (100 μM) had no effect on cloned Kv4.3 activity, aloperine (1 μM and up) negative-shifted the voltage dependence of hERG activation by ~10 mV and increased peak hERG current by 35%. Thus, aloperine exerts striking anti-arrhythmic effects against myocardial ischemia and reperfusion injury-induced severe lethal ventricular arrhythmia and sudden cardiac death via the ERK1/2/STAT-3 signaling pathway, with potential additional contribution from increased cardiac myocyte repolarization capacity via augmented hERG activity.
Liquid metal enabled microfluidics (2017)

Several gallium-based liquid metal alloys are liquid at room temperature. As 'liquid', such alloys have a low viscosity and a high surface tension while as 'metal', they have high thermal and electrical conductivities, similar to mercury. However, unlike mercury, these liquid metal alloys have low toxicity and a negligible vapor pressure, rendering them much safer. In comparison to mercury, the distinguishing feature of these alloys is the rapid formation of a self-limiting atomically thin layer of gallium oxide over their surface when exposed to oxygen. This oxide layer changes many physical and chemical properties of gallium alloys, including their interfacial and rheological properties, which can be employed and modulated for various applications in microfluidics. Injecting liquid metal into microfluidic structures has been extensively used to pattern and encapsulate highly deformable and reconfigurable electronic devices including electrodes, sensors, antennas, and interconnects. Likewise, the unique features of liquid metals have been employed for fabricating miniaturized microfluidic components including pumps, valves, heaters, and electrodes. In this review, we discuss liquid metal enabled microfluidic components, and highlight their desirable attributes including simple fabrication, facile integration, stretchability, reconfigurability, and low power consumption, with promising applications for highly integrated microfluidic systems.
Time resolved long-wave infrared laser-induced breakdown spectroscopy of inorganic energetic materials by a rapid mercury–cadmium–telluride linear array detection system (2016)

A mercury-cadmium-telluride linear array detection system that is capable of rapidly capturing (∼1-5 s) a broad spectrum of atomic and molecular laser-induced breakdown spectroscopy (LIBS) emissions in the long-wave infrared region (LWIR, ∼5.6-10 μm) was recently developed. Similar to the conventional ultraviolet-visible LIBS, a broadband emission spectrum of condensed phase samples covering a 5.6-10 μm spectral region could be acquired from just a single laser-induced micro-plasma. Intense and distinct atomic and molecular LWIR emission signatures of various solid inorganic energetic materials were readily observed and identified. Time resolved emissions of inorganic energetic materials were studied to assess the lifetimes of LWIR atomic and molecular emissions. The LWIR atomic emissions generally decayed fast on the scale of tens of microseconds, while the molecular signature emissions from target molecules excited by the laser-induced plasma appeared to be very long lived (∼millisecond). The time dependence of emission intensities and peak wavelengths of these signature emissions gave an insight into the origin and the environment of the emitting target species. Moreover, observed lifetimes of these LWIR emissions can be utilized for further optimization of the signal quality and detection limits of this technique.
Synthesis and Electrochemical Properties of a Novel Mono-meso-ferrocenyl Porphyrin (2006)

A synthetic route to mono-meso-ferrocenyl porphyrins using a MacDonald-type 2+2 condensation is described. In this method, the substituted diformyldipyrrylmethane 4 is treated with the dicarboxydipyrrylmethane 6. The 5-ferrocenyldipyrrylmethane was obtained by condensation of benzyl 3-methyl-4-ethylpyrrol-2carboxylate with ferrocenecarboxaldehyde in the presence of p-toluenesulfonic acid. The unequivocal assignment of all proton resonances in the new porphyrin was achieved by means of a detailed analysis of its H-H NOESY correlations. Interestingly, we noticed that the ferrocenyl group introduces an element of asymmetry in the molecule. The corresponding Co(II) and Ni(II) complexes were also prepared. In cyclic voltammograms of the free base and the Co or Ni complexes all voltammetric peaks disappear after four consecutive scans. This result suggests no film formation in any of them.
Fibroblast Growth Factors: Evolution (2006)

Abstract Fibroblast growth factors (FGFs) and their receptors constitute a signaling system conserved throughout animal evolution. Most members of the FGF family signal across adjacent tissue boundaries and are essential for a variety of developmental and physiologic processes. A subfamily of vertebrate FGFs that may have originated in primitive chordates appears to have evolved novel functions within neurons.
Claquage microonde dans un multiplexeur à deux canaux : mesures expérimentales et comparaison aux simulations numériques (2015)

National audience
Active stereo based surface reconstruction (2002)

The problem of surface reconstruction from stereo images for large scenes having large depth ranges is considered. The passive stereo paradigm is inadequate for this problem because of the need to aim cameras in different directions and to fixate on different objects. An active stereo approach, in which the scene is systematically scanned and image acquisition and surface reconstruction are integrated using a four-step process, is presented. First, a new fixation point is selected from among the nonfixated, low-resolution scene parts of current fixation. Then a reconfiguration of cameras is initiated for refixation. As reconfiguration progresses, the images of the new fixation point gradually deblur and the accuracy of the stereo estimate of the point improves. The improved stereo estimate is used to achieve accurate focus and vergence settings of the cameras for fixation. Finally, focus-based depth estimates are obtained at a grid near the fixation point whose density is determined by the local surface slope. These estimates are fused with those obtained from stereo using maximum likelihood, and are interpolated to the nongrid points.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Faseroptischer chemischer Multisensor zur medizinischen Patientenüberwachung (2013)

Zusammenfassung In diesem Beitrag beschreiben wir die ersten Resultate einer Studie, in der die Möglichkeit der Entwicklung eines miniaturisierten, hochflexiblen faseroptischen Sensors für mehrere chemische und physikalische Parameter geprüft wurde. Der Sensor sollte ausreichend schlank und flexibel sein, um selbst in empfindlichen Bereichen des menschlichen Körpers längere Zeit ohne Irritationen eingesetzt werden zu können. Eine mögliche Anwendung wäre die kontinuierliche, nichtinvasive Messung von Blutgaswerten zur Überwachung von Patienten in medizinischer Pflege. Das Design und die Mikrofabrikation des Sensors und des Auslesesystems werden beschrieben und Messungen von O
Palladium complexes with the tripodal phosphine tris-(2-diphenylphosphinoethyl)amine. Synthesis and structure of trigonal, tetrahedral, trigonal bipyramidal, and square planar complexes (1989)

The tripod-like ligand N(CH2CH2PPh2)3(tdpea) forms the palladium(0) trigonal complex [Pd(tdpea)]·0.5Me2CO, (1), in which the apical nitrogen atom of the phosphine is unco-ordinated. This unsaturated species undergoes both addition (CO, SO2) and oxidative-addition reactions (alkyl halides) to form respectively tetrahedral and trigonal bipyramidal complexes. The latter contain quadridentate tdpea. Complex (1) reacts with CO2 or CS2 in the presence of O2 to form a square planar carbonate or dithiocarbonate derivative, with oxidation of a terminal PPh2 group to OPPh2. Crystal data for (1) are: a= 10.827(8)Å, α= 108.82(9)°, space group R3, and Z= 1. In the molecule the metal atom displays an unprecedented out-of-plane trigonal co-ordination. Crystal data for [Pd(tdpea)Me]I are: a= 10.570(7)Å, α= 106.10(7)°, space group R3, and Z= 1. The geometry of the [Pd(tdpea) Me]+ cation is trigonal bipyramidal, with the three-fold crystallographic axis passing through the nitrogen, the palladium, and the carbon atom.
A Viral Vaccine Encoding Prostate-Specific Antigen Induces Antigen Spreading to a Common Set of Self-Proteins in Prostate Cancer Patients (2010)

Vaccine + EBRT induced antigen spreading in a large proportion of patients. A subset of patients developed autoantibodies to a panel of four self-antigens and showed a trend toward inferior outcomes. Thus, cancer vaccines directed against tumor-specific antigens can trigger autoantibody responses to self-proteins, which may influence the efficacy of vaccination.
Suicide Gene-Engineered Stromal Cells Reveal a Dynamic Regulation of Cancer Metastasis (2016)

Cancer-associated fibroblasts (CAFs) are a major cancer-promoting component in the tumor microenvironment (TME). The dynamic role of human CAFs in cancer progression has been ill-defined because human CAFs lack a unique marker needed for a cell-specific, promoter-driven knockout model. Here, we developed an engineered human CAF cell line with an inducible suicide gene to enable selective in vivo elimination of human CAFs at different stages of xenograft tumor development, effectively circumventing the challenge of targeting a cell-specific marker. Suicide-engineered CAFs were highly sensitive to apoptosis induction in vitro and in vivo by the addition of a simple small molecule inducer. Selection of timepoints for targeted CAF apoptosis in vivo during the progression of a human breast cancer xenograft model was guided by a bi-phasic host cytokine response that peaked at early timepoints after tumor implantation. Remarkably, we observed that the selective apoptosis of CAFs at these early timepoints did not affect primary tumor growth, but instead increased the presence of tumor-associated macrophages and the metastatic spread of breast cancer cells to the lung and bone. The study revealed a dynamic relationship between CAFs and cancer metastasis that has counter-intuitive ramifications for CAF-targeted therapy.
Mutations Causing Neurodegeneration in Caenorhabditis elegans Drastically Alter the pH Sensitivity and Inactivation of the Mammalian H+-gated Na+ Channel MDEG1 (1998)

The mammalian degenerin MDEG1 belongs to the nematode degenerin/epithelial Na+ channel superfamily. It is constitutively activated by the same mutations that cause gain-of-function of the Caenorhabditis elegans degenerins and neurodegeneration. ASIC and DRASIC, which were recently cloned, are structural homologues of MDEG1 and behave as H+-gated cation channels. MDEG1 is also a H+-activated Na+ channel, but it differs from ASIC in its lower pH sensitivity and slower kinetics. In addition to the generation of a constitutive current, mutations in MDEG1 also alter the properties of the H+-gated current. Replacement of Gly-430 in MDEG1 by bulkier amino acids, such as Val, Phe, or Thr, drastically increases the H+ sensitivity of the channel (half-maximal pH (pHm) approximately 4.4 for MDEG1, pHm approximately 6.7 for the different mutants). Furthermore, these replacements completely suppress the inactivation observed with the wild-type channel and increase the sensitivity of the H+-gated channel to blockade by amiloride by a factor of 10 without modification of its conductance and ionic selectivity. These results as well as those obtained with other mutants clearly indicate that the region surrounding Gly-430, situated just before the second transmembrane segment, is essential for pH sensitivity and gating.
Long-term neuroprotection of retinal ganglion cells by inhibiting caspase-2 (2016)

Caspases are a family of cysteine-rich proteases that activate apoptotic pathways through their ‘initiator’ or ‘executioner’ pathways. Initiator caspases activate executioner caspase, such as caspase-2, -3, -6 and -7, which hydrolyze or cleave structural proteins in the cell leading to apoptosis. Caspase-2 is the most highly conserved caspase and uniquely fits into both initiator and executioner categories and as such partakes in a variety of apoptosis inducing signals including DNA damage, heat shock, endoplasmic reticulum and oxidative stress. Caspase-2-deficient neurons are resistant to apoptosis induced by beta-amyloid although it is involved in apoptosis of hippocampal neurons after ischemia and is expressed in ischemic retinae.1
Figure S1 from Autophagy Sustains Mitochondrial Glutamine Metabolism and Growth of <i>Braf</i><sup>V600E</sup>–Driven Lung Tumors (2023)

&lt;p&gt;Figure S1 - PDF file 976K, Persistent deletion of ATG7 in late stage tumorigenesis&lt;/p&gt;
Surface display vectors for selective detection and isolation of high level antibody producing cells (2016)

Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS) based enrichment and cloning of antibody producing CHO cells, we developed a special vector setup by inserting a leaky translation termination signal between the heavy chain of an IgG antibody and an IgG transmembrane domain. Partial read-through during translation of the antibody heavy chain leads to display of a subset of the produced antibody on the surface of the expressing cell. We could show that the level of surface expression correlates well with the productivity. By applying FACS, high producing cells can be selectively enriched and cloned. Two sequential FACS enrichment cycles were performed which led to more than eightfold increased productivities of transfected and selected cell populations without cloning. The combination of selective FACS enrichment and FACS cloning with the new vector setup led to a sevenfold higher average productivity of the resulting clones as compared to a reference vector. Productivity and production stability assessment of clones generated with the new vector showed no negative impact of the co-expression of transmembrane antibody. Clone productivities of 4 g/L in a generic shake flask fed-batch model were achieved. Thus, this new vector setup facilitates fast and selective isolation of high producing production cell lines and allows significant reduction of clone screening efforts during cell line development for production cell lines. Additionally, the high productivity of FACS-enriched but non-clonal cell populations supports rapid, high yield, and cost efficient material production in early project phases. Biotechnol. Bioeng. 2016;113: 2386-2393. © 2016 Wiley Periodicals, Inc.
A Water Flow-Like Algorithm for the Travelling Salesman Problem (2014)

The water flow-like algorithm (WFA) is a relatively new metaheuristic that performs well on the object grouping problem encountered in combinatorial optimization. This paper presents a WFA for solving the travelling salesman problem (TSP) as a graph-based problem. The performance of the WFA on the TSP is evaluated using 23 TSP benchmark datasets and by comparing it with previous algorithms. The experimental results show that the proposed WFA found better solutions in terms of the average solution and the percentage deviation of the average solution from the best-known solution.