gebhart/patrepeval huggingface dataset:
Translation of glycoprotein IIIa in stored blood platelets (2007)

This article confirms that PLTs are capable of synthesizing biologically relevant proteins ex vivo throughout a 10-day storage period with particularly long-lived mRNA and provides a framework through which the biochemical mechanisms involved in the translational regulation of proteins thought to be involved in the initiation or exacerbation of the PLT storage lesion can be investigated.
Edge-based Adaptive Directional Intra Prediction (2010)

H.264/AVC employs intra prediction to reduce spatial redundancy between neighboring blocks. Different directional prediction modes are used to cater diversified video content. Although it achieves quite high coding efficiency, it is desirable to analyze its drawbacks in the existing video coding standard, since it allows us to design better ones. Basically, even after intra prediction, the residue still contains a lot of edge or texture information. Unfortunately, these high frequency components consume a large quantity of bits and the distortion is usually quite high. Based on this drawback, an Edge-based Adaptive Directional Intra Prediction is proposed (EADIP) to reduce the residue energy especially for the edge region. In particular, we establish an edge model in EADIP, which is quite flexible for natural images. Within the model, the edge splits the macroblock into two regions, each being predicted separately. In implementation, we consider the current trend of mode selection and complexity issues. A mode extension is made on INTRA 16 × 16 in H.264/AVC. Experimental results show that the proposed algorithm outperforms H.264/AVC. And the proposed mode is more likely to be chosen in low bitrate situations.
Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system (2009)

The enzymatic profile of the extracellular extract from F. oxysporum submerged cultures using BG and corn cob as the carbon source was proved efficient for a successful hydrolysis of BG. The fermentation study carried out using sugar mixtures simulating BG's carbohydrates content and consecutively alkali-pretreated and untreated BG, indicates that BG hydrolysis is the bottleneck of the bioconversion process. However, a considerable bioconversion yield was achieved (60% of the theoretical) making this bioprocess worthy of further investigation for a potential commercial application.
The Pore-Forming Protein Cry5B Elicits the Pathogenicity of Bacillus sp. against Caenorhabditis elegans (2011)

The soil bacterium Bacillus thuringiensis is a pathogen of insects and nematodes and is very closely related to, if not the same species as, Bacillus cereus and Bacillus anthracis. The defining characteristic of B. thuringiensis that sets it apart from B. cereus and B. anthracis is the production of crystal (Cry) proteins, which are pore-forming toxins or pore-forming proteins (PFPs). Although it is known that PFPs are important virulence factors since their elimination results in reduced virulence of many pathogenic bacteria, the functions by which PFPs promote virulence are incompletely understood. Here we study the effect of Cry proteins in B. thuringiensis pathogenesis of the nematode Caenorhabditis elegans. We find that whereas B. thuringiensis on its own is not able to infect C. elegans, the addition of the PFP Cry protein, Cry5B, results in a robust lethal infection that consumes the nematode host in 1-2 days, leading to a "Bob" or bag-of-bacteria phenotype. Unlike other infections of C. elegans characterized to date, the infection by B. thuringiensis shows dose-dependency based on bacterial inoculum size and based on PFP concentration. Although the infection process takes 1-2 days, the PFP-instigated infection process is irreversibly established within 15 minutes of initial exposure. Remarkably, treatment of C. elegans with Cry5B PFP is able to instigate many other Bacillus species, including B. anthracis and even "non-pathogenic" Bacillus subtilis, to become lethal and infectious agents to C. elegans. Co-culturing of Cry5B-expressing B. thuringiensis with B. anthracis can result in lethal infection of C. elegans by B. anthracis. Our data demonstrate that one potential property of PFPs is to sensitize the host to bacterial infection and further that C. elegans and probably other roundworms can be common hosts for B. cereus-group bacteria, findings with important ecological and research implications.
Vaccine-induced immune thrombotic thrombocytopenia: what we know and do not know (2021)

The development of vaccines to fight COVID-19 has been a remarkable medical achievement. However, this global immunization effort has been complicated by a rare vaccine-related outcome characterized by thrombocytopenia and thrombosis in association with platelet-activating anti-platelet factor 4 antibodies. In this Spotlight, we will discuss the recently described complication of vaccine-induced immune thrombotic thrombocytopenia (VITT) occurring in response to certain COVID-19 vaccines. Although information about this clinical condition is rapidly evolving, we will summarize our current understanding of VITT.
Cell-Mediated Cytotoxicity Against Virus-Infected Target Cells in Humans (1978)

Abstract In a 51Chromium release assay performed without immune sera, purified lymphocytes from randomly selected healthy donors killed significantly more allogeneic virus-infected than noninfected fibroblasts. An incubation period of 18 to 20 hr was optimal for determining cytotoxicity, but increased cytotoxic activity against infected cells was observed as early as 6 hr from the start of the test. No correlation could be found between donors' antibody titers to any virus tested and the cytotoxic efficiency of their effector cells: virus-infected cells were lysed by both seropositive and seronegative individuals. With several different cell fractionation techniques, the effector cell in this system could not be distinguished from the human natural killer cell, which is a lymphocyte with receptors for the Fc fragment of IgG but with no surface immunoglobulin. When lymphocytes were separated on the basis of ability to form rosettes with neuraminidase or AET (2-aminoethylisothiouronium bromide hydrobromide)-treated sheep erythrocytes, the majority of cytotoxic activity was consistently recovered in the nonrosetting fraction. A portion of it, however, was always present in the rosetting fraction and was, again, mediated by lymphocytes carrying receptors for the Fc fragment of IgG.
Data from Maintenance of Constitutive IκB Kinase Activity by Glycogen Synthase Kinase-3α/β in Pancreatic Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;Constitutive nuclear factor κB (NF-κB) activation is among the many deregulated signaling pathways that are proposed to drive pancreatic cancer cell growth and survival. Recent reports suggest that glycogen synthase kinase-3β (GSK-3β) plays a key role in maintaining basal NF-κB target gene expression and cell survival in pancreatic cancer cell lines. However, the mechanism by which GSK-3β facilitates constitutive NF-κB signaling in pancreatic cancer remains unclear. In this report, we analyze the contributions of both GSK-3 isoforms (GSK-3α and GSK-3β) in regulating NF-κB activation and cell proliferation in pancreatic cancer cell lines (Panc-1 and MiaPaCa-2). We show that GSK-3 isoforms are differentially required to maintain basal NF-κB DNA binding activity, transcriptional activity, and cell proliferation in Panc-1 and MiaPaCa-2 cells. Our data also indicate that IκB kinase (IKK) subunits are not equally required to regulate pancreatic cancer–associated NF-κB activity and cell growth. Importantly, we provide the first evidence that GSK-3 maintains constitutive NF-κB signaling in pancreatic cancer by regulating IKK activity. These data provide new insight into GSK-3–dependent NF-κB regulation and further establish GSK-3 and IKK as potential therapeutic targets for pancreatic cancer. [Cancer Res 2008;68(19):8156–63]&lt;/p&gt;&lt;/div&gt;
Epidural analgesia practices for labour: results of a 2005 national survey in Ireland (2009)

It appears that Irish obstetric anaesthetists have adopted the low-dose epidural analgesia trend for the maintenance of labour analgesia. This practice is not as widespread, however, for test dosing, the induction of analgesia dose or in the administration of intermittent epidural boluses to maintain analgesia when higher concentrations are used. Since its introduction in 2000, levobupivacaine has become the most popular local anaesthetic agent.
Supplementary Figures 1-7, Table 1 from MAGE-A, mMage-b, and MAGE-C Proteins Form Complexes with KAP1 and Suppress p53-Dependent Apoptosis in MAGE-Positive Cell Lines (2023)

Supplementary Figures 1-7, Table 1 from MAGE-A, mMage-b, and MAGE-C Proteins Form Complexes with KAP1 and Suppress p53-Dependent Apoptosis in MAGE-Positive Cell Lines
Human primary and memory cytotoxic T lymphocyte responses are efficiently induced by means of CD40-activated B cells as antigen-presenting cells: potential for clinical application (2002)

CD40 engagement is the major signal that induces B cells to efficiently present antigen to T cells. We previously demonstrated that human peripheral blood-derived CD40-activated B cells (CD40-B cells) function as antigen-presenting cells (APCs). Here, we have established a culture system to generate these APCs under clinically applicable conditions using guanylic acid-grade soluble trimeric CD40 ligand. To monitor APC function and antigen loading for these cells, simple and efficient quality control assays have been developed. Using this approach, we demonstrate that CD40-B cells from healthy donors and cancer patients are fully functional and equally expanded in long-term cultures. These B cells boost robust memory T-cell responses, but more importantly, they also prime naive T-cell responses against neoantigens ex vivo. CD40-B cells overcome current obstacles, such as the difficulty of isolation, generation, and long-term expansion observed with other APCs. Therefore, they are an excellent source of professional APCs for immune assessment, antigen discovery, and antigen-specific immunotherapy.
A study of the crystal chemistry, electron density distributions, and hydrogen incorporation in the Al₂SiO₅ polymorphs (2006)

The Al₂SiO₅ polymorphs have been examined to provide new insights into their chemical bonding, their crystal chemistry, their equations of state, and the incorporation of water in the form of hydroxyl in their structures. The Al₂SiO₅ polymorphs provide a unique structural assemblage for a crystal chemical examination due to the variation in Al coordination in the structures where Al is in 4-fold, 5-fold, and 6-fold in sillimanite, andalusite, and kyanite, respectively. Consequently, the Al₂SiO₅ polymorphs have been examined with a combination of experimental (high pressure X-ray diffraction and Polarized FTIR spectroscopy) and theoretical (VASP and Crystal 98) methods. An experimental high pressure X-ray diffraction study on andalusite and sillimanite has constrained their equation of state and the pressure derivatives of their bulk modulus with pressure. Additionally, the effect of pressure on the crystal structures has been examined, where the main structural response is compression of the AlO₆ octahedra. Comparatively, compression of the AlO₆ octahedra in andalusite is more anisotropic, while the major direction of axial compressibility in both structures is dependent on the orientation of the AlO6 octahedra. In order to better understand the crystal chemistry of the Al-O and Si-O bonds in the polymorphs, ELF isosurfaces were examined. ELF isosurfaces represent a graphical representation of the localized electron probability density. Six distinct types of ELF isosurfaces were observed in the Al₂SiO₅ polymorphs resulting from differences in the geometry, coordination, and coordinated cation atomic number surrounding the oxygens within the crystal structures. The ELF was also shown to be isostructurally related to electron density difference maps. In a combined experimental and theoretical investigation of the Al₂SiO₅ polymorphs, potential protonation sites within the crystal structures were determined at an atomic level with polarized FTIR spectroscopy and analysis of (3,-3) critical points of the negative Laplacian. The polarized FTIR spectra indicate the orientation of the OH dipole in the three polymorphs and the (3,-3) critical points indicate regions of locally concentrated electron density. Potential protonation sites were determined based on the value of the negative Laplacian, the underbonded nature of the oxygens, and the number of surrounding cations.
Size Spectra and Atmospheric Growth of V-Containing Aerosol in Washington, DC (1996)

ABSTRACT Size-segregated submicrometer aerosol particles were collected with micro-orifice impactors (MOI) at three sites in the heavily urban, but nonindustrial-ized Washington, DC metropolitan area during a 40-day period in August and September of 1990, when atmospheric V (an important marker of oil combustion emissions) was principally derived from commercial and utility oil combustion. Results for 34 MOI samples, analyzed for V by instrumental neutron activation analysis, were fit with a least-squares technique which used impactor calibration data to determine log-normal distribution parameters, i.e., mass median aerodynamic diameter (mmad) and geometric standard deviation σg; for fine particles bearing V. Geometric size distribution parameters were also determined. The median mmad for 19 College Park (CP) samples was 0.361 ± 0.006 μm. At this site, mmads for samples collected in the absence of rain and with V concentrations > 0.61 ng/m3 increased continuously with increasing relative humidity (RH) from 56% to 79% according to the equation dp 3= −0.0222 ± 0.0033/ln(aw) − 0.013 ± 0.009. Mmads for samples collected at Andrews AFB were characteristically smaller than those determined at CP at comparable RH, probably due to the influence of a nearby oil-fired boiler.
A novel approach for making energy efficient PON (2011)

Nowadays Passive Optical Network (PON) requires that Optical Network Units (ONUs) wake up periodically to check if the Optical Line Terminal (OLT) has any message directed to them. This implies that ONUs change from sleeping mode in which they just consume 1 W to active mode in which the consumption goes up to 10 W. In many cases, the OLT does not have any packets for the ONU and it goes to sleep again, what supposes a waste of energy. In this paper, we propose a novel Hybrid ONU that relies on a low-cost and low-energy technology, IEEE 802.15.4, to wake up those ONUs that are going to receive a packet. Our first estimations demonstrates that our solution would save around 25000$ per year and OLT.
Highly resistive GaN layers formed by ion implantation of Zn along the c axis (2003)

Highly resistive layers are formed by the implantation of Zn ion along the c axis of GaN and AlGaN/GaN epitaxial layers. Heavy ions such as Zn have been desirable for the formation of highly resistive layers, because ions effectively transferred their energy to the crystal atoms rather than the electrons in GaN. A sheet resistance Rs as high as 3.8×1011 Ω/sq was obtained on GaN layers after the ion implantation. Rs increased up to 2.2×1013 Ω/sq after the annealing at 500 °C for 300 s in an N2 atmosphere. The thermal activation energy Er for this sample was 0.67 eV. It was found that the experimental data in current–voltage characteristics were fitted to the equation included the Poole–Frenkel current and resistive (ohmic) current. The difference of Rs between the as-implanted and 500 °C annealed samples was due to the Poole–Frenkel current. The Poole–Frenkel current overcame the resistive one, and dominated the current mechanism in the case of the samples annealed at 200 °C or less. On the other hand, for the samples annealed at 500 °C, the current was only resistive. Both Rs and Er decreased as the annealing temperature increased above 500 °C. Furthermore this implantation method was applied to the device isolation of AlGaN/GaN high electron mobility transistors (HEMTs). The high Rs of 5.9×1011 Ω/sq was observed for AlGaN/GaN structures as well as GaN layers which were not annealed. The HEMTs with a gate length of 1 μm had a high drain current of over 1 A/mm at the gate voltage of 1 V and a pinch off voltage of −7 V without a harmful leakage current.
A new approach to scalable video coding (2002)

This paper introduces a new framework for video coding that facilitates operation over a wide range of transmission rates. The new method is a subband coding approach that employs motion compensation, and uses prediction-frame and intra-frame coding within the framework. It is unique in that it allows lossy coding of the motion vectors through its use of multistage residual vector quantization (RVQ). Furthermore, it selects the motion vector with the best rate-distortion tradeoff among a number of possible motion vector candidates, and provides a rate-distortion-based mechanism for alternating between intra-frame and inter-frame coding. The framework provides an easy way to control the system complexity and performance, and inherently supports multiresolution transmission.
Withaferin A protects against hyperuricemia induced kidney injury and its possible mechanisms (2021)

The study was designed to explore the effects of Withaferin A (WFA) on hyperuricemia-induced kidney injury and its action mechanism. Potassium oxonate (PO) was employed to establish the hyperuricemic mouse model. The pathological changes of renal tissue were evaluated by hematoxylin-eosin and masson trichrome staining. The levels of creatinine, blood urea nitrogen (BUN), uric acid (UA) and xanthine oxidase (XOD) were detected using corresponding commercial kits. Expressions of collagen-related and apoptosis-associated proteins in renal tissues were, respectively, evaluated by immunofluorescence and western blotting. Cell apoptosis was detected by TUNEL assay, and transporter expressions using western blotting. Followed by WFA, NRK-52E cells were treated with UA before evaluation of apoptosis and fibrosis. Results indicated that WFA ameliorated renal damage, improved kidney function, and decreased levels of creatinine, BUN, UA, and XOD in PO-induced hyperuricemic mice. Furthermore, WFA significantly prevented renal fibrosis and increased the expression of collagen-related proteins. Similarly, WFA markedly inhibited renal apoptosis, accompanied by changes of apoptosis-related proteins. Importantly, expression of transporters responsible for the secretion of organic anion transporter 1 (OAT1), OAT3, ATP-binding cassette subfamily G member 2 (ABCG2) was remarkably enhanced whereas that of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) was reduced in renal tissues of mice with hyperuricemia. In vitro study revealed that WFA notably ameliorated UA-induced cell fibrosis and apoptosis. Taken together, WFA improves kidney function by decreasing UA via regulation of XOD and transporter genes in renal tubular cells.
Asymmetric Synthesis of Isoindoline and Isoquinoline Derivatives Using Nickel(0)-Catalyzed [2 + 2 + 2] Cocyclization (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric Synthesis of Isoindoline and Isoquinoline Derivatives Using Nickel(0)-Catalyzed [2 + 2 + 2] CocyclizationYoshihiro Sato, Toyoki Nishimata, and Miwako MoriCite this: J. Org. Chem. 1994, 59, 21, 6133–6135Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/jo00100a003https://doi.org/10.1021/jo00100a003research-articleACS PublicationsRequest reuse permissionsArticle Views1571Altmetric-Citations159LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Polymerization photosensitized by carbonyl compounds (1979)

Abstract The polymerization of methyl methacrylate and styrene photosensitized by acetone, aldehydes, ethyl pyruvate, 2,3‐butanedione, and 2,3‐pentanedione has been investigated and the effect of several additives (carbon tetrachloride, cumene, diethyl amine, triethyl amine, 2‐pentanol, and tetrahydrofuran) on initiation efficiency has been evaluated. The initiation efficiency of a given system depends on several factors, the most important of which are the relative rates of quenching by the monomers and the additives and type of product obtained.
Dual Function of Mitochondrial Nm23-H4 Protein in Phosphotransfer and Intermembrane Lipid Transfer (2012)

The nucleoside diphosphate kinase Nm23-H4/NDPK-D forms symmetrical hexameric complexes in the mitochondrial intermembrane space with phosphotransfer activity using mitochondrial ATP to regenerate nucleoside triphosphates. We demonstrate the complex formation between Nm23-H4 and mitochondrial GTPase OPA1 in rat liver, suggesting its involvement in local and direct GTP delivery. Similar to OPA1, Nm23-H4 is further known to strongly bind in vitro to anionic phospholipids, mainly cardiolipin, and in vivo to the inner mitochondrial membrane. We show here that such protein-lipid complexes inhibit nucleoside diphosphate kinase activity but are necessary for another function of Nm23-H4, selective intermembrane lipid transfer. Mitochondrial lipid distribution was analyzed by liquid chromatography-mass spectrometry using HeLa cells expressing either wild-type Nm23-H4 or a membrane binding-deficient mutant at a site predicted based on molecular modeling to be crucial for cardiolipin binding and transfer mechanism. We found that wild type, but not the mutant enzyme, selectively increased the content of cardiolipin in the outer mitochondrial membrane, but the distribution of other more abundant phospholipids (e.g. phosphatidylcholine) remained unchanged. HeLa cells expressing the wild-type enzyme showed increased accumulation of Bax in mitochondria and were sensitized to rotenone-induced apoptosis as revealed by stimulated release of cytochrome c into the cytosol, elevated caspase 3/7 activity, and increased annexin V binding. Based on these data and molecular modeling, we propose that Nm23-H4 acts as a lipid-dependent mitochondrial switch with dual function in phosphotransfer serving local GTP supply and cardiolipin transfer for apoptotic signaling and putative other functions.