gebhart/patrepeval huggingface dataset:
P2.2.10 Development of Guided SH-SAW based Wireless Sensing Platform for Monitoring Protein Binding (2012)

In this study, a wireless biosensing platform was developed for the detection of protein binding. The system consists of a layered ZnO/36° YX-LiTaO3 SH-SAW device and a microfluidic flow cell. The influence of the interactions between protein A and mouse IgG on the characteristics of the SH-SAW device was measured and analyzed for varying concentrations of mouse IgG. The experimental results demonstrated that the insertion loss of the device increased from -46.8 dB to -50.9 dB and the center frequency of the device decreased from 94.56 MHz to 94.49 MHz as the mouse IgG concentration was increased from 1 µM to 40 µM, respectively. This sensor platform enabled real time monitoring of protein binding within a microfluidic flow cell.
Oxygen Permeability (Dk) of Thirty-Seven Rigid Contact Lens Materials (2002)

Omission of a single outlying point from a linear resistance vs. thickness regression can help provide a more valid Dk value. The ANSI Z80.20-1998 tolerance of +/-20% on Dk and the measurement reproducibility of +/-10% were achieved for the overwhelming majority of rigid contact lens materials up to at least 160 Dk units. The corrected, calibrated Dk values for the 37 test materials, in ANSI units, ranged from 13.8 to 175.1, having an overall mean of 43.2, median of 31.9, and standard error of 6.57 (N = 37).
Improved capacitance measurement by means of resonance locking (1994)

We describe a new LC method of capacitance measurement. The method consists of a synthesis of LC resonance and LC oscillator methods. The LC tank circuit is driven by a voltage-controlled oscillator (vco) and is included in an analogue phase-locked loop; the loop is designed to lock with no phase difference between current flow and driving voltage in the tank circuit. The system effectively tracks the resonance peak as the capacitance changes, thus allowing automatic measurement of capacitance by monitoring the vco input voltage or output frequency. The measurement bandwidth is dependent only on phase detector response, and can extend to the range of several MHz. The accuracy of this 'resonance locked loop' is equivalent to that of conventional systems and the dynamic range is considerably improved; a system with measured accuracy of 11 aF and bandwidth of 100 kHz has been constructed, and has been used to measure high-frequency submicrometre movements of piezoelectric actuators.
Advanced turbine design for coal-fueled engines. Topical report, Task 1.6, Task 1.7 (1992)

The objective of this task is to perform a technical assessment of turbine blading for advanced second generation PFBC conditions, identify specific problems/issues, and recommend an approach for solving any problems identified. A literature search was conducted, problems associated with hot corrosion defined and limited experiments performed. Sulfidation corrosion occurs in industrial, marine and aircraft gas turbine engines and is due to the presence of condensed alkali (sodium) sulfates. The principle source of the alkali in industrial, marine and aircraft gas turbine engines is sea salt crystals. The principle source of the sulfur is not the liquid fuels, but the same ocean born crystals. Moreover deposition of the corrosive salt occurs primarily by a non-equilibrium process. Sodium will be present in the cleaned combusted gases that enter the PFBC turbine. Although equilibrium condensation is not favored, deposition via impaction is probable. Marine gas turbines operate in sodium chloride rich environments without experiencing the accelerated attack noted in coal fired boilers where condensed chlorides contact metallic surfaces. The sulfates of calcium and magnesium are the products of the reactions used to control sulfur. Based upon industrial gas turbine experience and laboratory tests, calcium and magnesium sulfates are, at temperatures up to 1500{degrees}F (815{degrees}C), relatively innocuous salts. In this study it is found that at 1650{degrees}F (900{degrees}C) and above, calcium sulfate becomes an aggressive corrodent.
Advanced generation anti‐prostate specific membrane antigen designer T Cells for prostate cancer immunotherapy (2013)

Our results demonstrate that 2nd gen anti-PSMA designer T cells exhibit superior antitumor functions versus 1st gen, providing a rationale for advancing this improved agent toward clinical application in prostate cancer immunotherapy.
Nucleotide sequence of the mitochondrial genes coding for tRNAGGRglyand tRNAGURval (1980)

Yeast mitochondrial DNA-pBR322 recombinant DNA molecules known to contain tRNA genes from a tRNA rich region of the yeast genome were used as a source of DNA for restriction mapping and tRNA gene sequence analysis. We report here restriction maps of two segments of yeast mitochondrial DNA and the sequence of mitochondrial genes coding for tRNAglyGGR and tRNAvalGUR. Both genes are flanked by A + T rich DNA and neither has an intervening sequence nor codes for a 3' CCA end. The tRNA structures deduced from the genes have the usual cloverleaf structures and invariant nucleotides. This combination of DNA sequencing and restriction mapping has enabled us to determine that the tRNAvalGUR and a previously sequenced tRNA, the tRNApheUUY are transcribed from the same strand of DNA.
LILRB4 expression in chronic myelomonocytic leukemia and myelodysplastic syndrome based on response to hypomethylating agents (2020)

LILRB4 is expressed in AML M4/M5 cells and negatively regulates immune cell activation <i>via</i> T-cell suppression. Its expression and role in chronic myelomonocytic leukemia (CMML) and myelodysplastic syndrome (MDS) are unknown. We investigated LILRB4 expression in 19 CMML and 27 MDS patients and correlated it with response to subsequent hypomethylating agent (HMA) therapy. LILRB4 RNA expression was increased in CMML patients when compared to MDS patients and healthy controls (<i>q</i> < 0.1) and slightly increased in patients who responded to HMAs (<i>q</i> > 0.1). Pathway analysis revealed upregulation of PD-1 signaling, CTLA-4 signaling, and inflammatory response, and gene correlates were positively associated with CTLA-4 expression. Given current modest results with immunotherapy in myeloid malignancies, further investigation of LILRB4 as an immune checkpoint inhibitor target is needed. With the positive correlation between LILRB4 and CTLA-4 expression, combining anti-LILRB4 and anti-CTLA-4 agents may be a novel therapeutic approach in myeloid malignancies that warrants larger studies.
Adrenal steroid precursors exert potent androgenic action in the hamster sebaceous glands of flank organs and ears (1996)

To assess the effect of the androgen precursors dehydroepiandrosterone (DHEA) and androstenedione (4-ene-dione) on androgen-sensitive parameters in the skin of the hamster, these two steroids were released from SILASTIC implants inserted sc into castrated male hamsters. The pigmented area of the flank organs, the size of the underlying sebaceous glands, [3H]thymidine incorporation into these sebaceous glands, and the size of the ear sebaceous glands were measured. The decrease in flank organ size caused by orchidectomy was partially reversed by DHEA and completely reversed by 4-ene-dione, testosterone (T), or dihydrotestosterone (DHT) implants. Similarly, the size of the sebaceous glands of the flank organs was reduced 88.1% by castration, whereas DHEA, 4-ene-dione, T, and DHT implants reversed the effect of orchidectomy. Orchidectomy also decreased the sizes of the sebaceous glands of the ears; DHEA, 4-ene-dione, T, and DHT implants increased their sizes to 50.6%, 81.9%, 91.6%, and 105.8% of the values found in intact hamsters, respectively. Parallel results were observed on the labeling of flank organ sebaceous glands with [3H]thymidine as well as on prostate weight. Serum concentrations of T and DHT became undetectable in castrated hamsters and were increased to intact or slightly elevated values in animals receiving implants of DHEA, 4-ene-dione, or T. The present results show that DHEA and 4-ene-dione are potent stimulators of androgen-sensitive parameters in the sebaceous glands of both the flank organs and ears in the hamster and illustrate the importance of extragonadal or peripheral intracrine formation of active steroids. It is suggested that the castrated hamster supplemented with adrenal precursor steroids is a good model that can closely mimic the human situation, where adrenal steroids play an important role in androgen formation and action in peripheral tissues.
Ultraviolet-sensitive Targets in the Enzyme-synthesizing Apparatus ofEscherichia coli (1967)

Inhibition by ultraviolet light of beta-galactosidase and alkaline phosphatase synthesis was investigated in both ultraviolet (UV)-sensitive and UV-resistant (wild-type) Escherichia coli, with the objective of determining the sensitivity of various targets. Kinetics of enzyme formation by unmated bacteria and in mating systems, in which the donor provided the specific genetic material and the recipient the cytoplasm, permit the following conclusions regarding the sensitivity of various targets. Catabolite repression resulting from UV damage causes most of the inhibition of beta-galactosidase formation. When it is largely eliminated by a step-down in nutrition, the principal target in UV-sensitive bacteria appears to be the structural gene (lacZ(+)), but damage to the cytoplasm is also important. Transitory inhibition by inactivation of messenger ribonucleic acid is also observed. In wild-type bacteria, repair reduces the importance of lesions in deoxyribonucleic acid sufficiently that cytoplasmic damage appears to be at least as important. Repair occurs within 10 min, as shown by recovery of enzyme-synthesizing ability. Caffeine and proflavine prevent recovery. Newly mated bacteria respond to irradiation very differently than do unmated bacteria. The beta-galactosidase or alkaline phosphatase structural gene (lacZ(+) or phoP(+)) is much more inhibited after it is transferred than it is in unmated bacteria. This sensitivity seems to depend on a sensitive state of the injected material, rather than on a different physiological condition of the entire zygote. Irradiation of recipient uvr(+) bacteria much more strongly inhibited expression of injected genes than if the F(-) was uvr(s). Studies on mating systems are not very useful for learning about the function of unmated bacteria.
The Benefit of Cardiac Resynchronization Therapy and QRS Duration: A Meta‐Analysis (2011)

The benefit of CRT seems to be dependent on QRS duration. Available data suggest a significant benefit associated with CRT in patients with QRS ≥ 150 ms, but not in patients with QRS < 150 ms. Further studies are needed to identify patients with QRS < 150 ms who might benefit from CRT.
Full-color video and still imaging using two flat lenses (2018)

At least two lenses are required in imaging systems to adjust focus and magnification and to match the acceptance properties of the image sensor. Here, we demonstrate the tandem use of two millimeter-scale planar diffractive lenses for video and still imaging over the full visible spectrum. First we designed, fabricated, and characterized individual lenses with focal length of 25mm and NA = 0.05. Then, we assembled the lenses with a conventional color CMOS image sensor to demonstrate imaging. Illumination conditions as varied as ambient sunlight, halogen lamp and LED lamps are used. Due to the ease of fabrication of diffractive lenses compared to metalenses, this demonstration will enable future generations of low-cost, lightweight, broadband cameras.
Direct gene transfer in Brassica napus (1986)

Direct gene transfer in Brassica napus. International Symposium Plant Molecular Biology CNRS/INRA
Potential benefits of implementing load control (2003)

The design of wholesale electricity markets through deregulation has focused almost exclusively on the development of competitive supply (generation). The demand side of the market has been virtually ignored. Mostly, this is due to the assumption that electricity demand is almost completely inelastic. As a result, deregulated wholesale markets universally fail to pass price signals down to the end-users. This paper challenges the assumption of inelastic demand by exploring the potential benefits of implementing a simple load control scheme. This load control scheme allows consumers to shift demand from high priced hours to low priced hours during the day. The benefits to the individual consumer are explored through an example applied to residential air conditioning using price and demand data from California. This example shows that "smart" use of air conditioning can lead to great savings for residential consumers, without sacrificing comfort. The potential for multiple consumers implementing load control to reduce wholesale prices is also examined.
Building Information Modeling Based Integration and Visualization for Facilities Management (2012)

Facilities managers need to identify and understand deterioration/failure causes-effect patterns in order to prepare preventive maintenance plans. However, this task is difficult because of the complex interaction between different building components and their operational conditions. In spite of the availability of advanced Computerized Maintenance Management Systems (CMMSs), these systems focus on the data management aspects (i.e. asset inventory, work orders and resource management) and lack the functions necessary to analyze the collected data in order to identify deterioration/failure patterns. Standardization based on Building Information Modeling (BIM) provides new opportunities to improve the efficiency of Facilities Management (FM) by sharing and exchanging building information between different applications throughout the lifecycle of the facilities. This research proposes the integration of FM related data from different sources such as CMMS and BIM through adding the CMMS inspection and maintenance data to the BIM database. This integration, in addition to defined logical and spatial relationships between components and spaces, is used to find the cause-effect relationships through a BIM-based visual analytics. This method facilitates heuristic problem solving and helps to increase the quality of O&amp;M phase. It is also proposed to integrate CMMS, BIM, video, and Real Time Location System (RTLS) data using Augmented Reality (AR) techniques to visualize construction and renovation operations and to retrieve information about the time, space, and related activities to visualize construction and renovation operations. Several case studies are implemented and tested to show the feasibility of the proposed methods.
Let Us Dance Just a Little Bit More --- On the Information Capacity of the Human Motor System (2011)

Fitts' law is a fundamental tool in measuring the capacity of the human motor system. However, it is, by definition, limited to aimed movements toward spatially expanded targets. We revisit its information-theoretic basis with the goal of generalizing it into unconstrained trained movement such as dance and sports. The proposed new measure is based on a subject's ability to accurately reproduce a complex movement pattern. We demonstrate our framework using motion-capture data from professional dance performances.
B-Cell Maturation Antigen (BCMA) Activation Exerts Specific Proinflammatory Effects in Normal Human Keratinocytes and Is Preferentially Expressed in Inflammatory Skin Pathologies (2011)

TNFα is known to be expressed in human skin, regulating immune-related responses. Here we report that human normal skin keratinocytes express the members of the TNF superfamily members A proliferation-inducing ligand (APRIL; TNFSF13), B cell-activating factor (BAFF; TNFSF13B), and their receptors, B cell maturation antigen (BCMA; TNFRSF17) and transmembrane activator, calcium-modulator, and cyclophilin ligand interactor (TACI; TNFRSF13B), in a distinct spatial pattern. Our data show a differential expression of these molecules within epidermal layers and skin appendages, whereas the BAFF-specific receptor BAFFR (TNFRSF13C) is absent. Importantly, APRIL and BCMA but not BAFF or TACI are up-regulated in inflammatory skin lesions of psoriasis and squamous cell carcinomas. To explore the functional significance of this system in the skin, we assayed these receptors and ligands in cultured primary keratinocytes and HaCaT cells. We show that both cell types express BAFF, APRIL, BCMA, and TACI. Furthermore, APRIL and/or BAFF trigger nuclear factor-κB activation and IL-6 and granulocyte macrophage colony-stimulating factor (GM-CSF) expression through functional BCMA receptors, an activation inhibited by anti-BCMA short hairpin RNA. However, BAFF and/or APRIL do not induce IL-8 or TNFα production. Our data advance BCMA as an inflammation-related TNFSFR member in keratinocytes, of potential importance in the management of inflammatory skin conditions.
ChemInform Abstract: A Facile One‐Carbon Homologation of Aryl Aldehydes to Amides. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Natural Products Active in Aberrant c‐Kit Signaling (2007)

Development of modulators of constitutively active, kinase domain mutants of c-Kit has proved to be very difficult. Therefore, a high-throughput differential cytotoxicity assay was developed to screen for compounds that preferentially kill cells expressing constitutively active c-Kit. The cells used in the assay, murine IC2 mast cells, express either the D814Y activating mutation (functionally equivalent to human D816Y) or wild type protein. This assay is robust and highly reproducible. When applied to libraries of natural product extracts (followed by assay-guided fractionation), two differentially active compounds were identified. To assess possible mechanisms of action, the active compounds were tested for inhibitory activity against a panel of signaling enzymes (including wild type and mutant c-Kit). Neither of the compounds significantly affected any of the 73 enzymes tested. The effects of commercially available modulators of known signaling components were also assessed using the screening assay. None of these inhibitors reproduced the differential activity seen with the natural products. Finally, both compounds were found to affect mitochondrial potential in cells expressing c-Kit(D814Y). These results suggest that the newly identified natural products may provide new avenues for intervention in aberrant c-Kit signaling pathways.
A case of recurrent renal vein thrombosis associated with idiopathic membranous nephropathy in an 11-year-old boy (2009)

症例は11歳，男子。基礎疾患はなく，腎静脈血栓症の合併を契機に，膜性腎症と診断された。血栓溶解療法のみでは腎静脈血栓症の再燃を繰り返したため，ネフローゼ症候群の治療としてステロイド剤，免疫抑制剤を併用し，症状が寛解した。 シクロスポリン投与中はネフローゼ症候群も寛解維持でき，血栓再燃もみられなかったが，シクロスポリン投与2年後の腎病理像では基底膜の肥厚の進行，足細胞下のElectron Dense Depositの増加があり膜性腎症StageIIからIIIへの進行がみられた。肝炎ウィルスをはじめ感染症スクリーニングは陰性，抗核抗体ならびに抗DNA抗体も陰性であり，現在のところ特発性膜性腎症と診断している。しかし，全身性エリテマトーデスなど自己免疫疾患については腎症を発症してしばらくしてから腎外症状が揃い確定診断に至る例の報告もあり，今後も念頭において慎重にフォローアップしていく必要があると考える。
A Mild and Facile Reduction of Azides to Amines by N,N-Dimethylhydrazine and Catalytic Ferric Chloride (1998)

Abstract Reaction of a variety of azido compounds with N,N-dimethylhydrazine in the presence of a catalytic amount of ferric chloride hexahydrate in methanol results in excellent yields of the corresponding amino compounds. This reductive system is compatible with a wide assortment of functional groups and has also been extended towards the synthesis of pyrrolo[2,1-c][1,4]benzodiazepine antibiotics.
LCP1 Regulates Cell Motility in Chondrosarcoma and Correlates with Metastatic Potential and Poor Patient Outcomes (2023)

Abstract Chondrosarcoma (CSA) is the second most common primary malignancy of bone, whose aggressive potential and chemo-resistant nature result in extremely poor outcomes in patients with advanced disease. Grading and prognostication of these tumors remain a significant challenge for pathologists, and medical oncologists have no effective therapies to prevent or treat metastatic disease. In the present study, we sought to explore the pathogenesis and aggressive progression of chondrosarcoma by comparing gene expression differences from metastasizing and non-metastasizing CSA tumors. We hypothesized that metastasizing tumors have inherent differences that may be attributed to the dysregulation of specific oncogenic cell signaling pathways. RNA-Seq analysis from patient-derived low-passage cell lines of metastasizing and non-metastasizing tumors pinpointed the gene LCP1 (lymphocyte cytosolic protein 1) as upregulated in CSA cells from metastasizing tumors. Analysis of a large clinical cohort of CSA demonstrated that increased expression of LCP1 correlated with poor patient survival. In vitro analyses confirmed the ability of LCP1 to promote migration and invasion of CSA cells. These data support the key role of LCP1 on metastatic potential and poor prognosis in CSA. In conclusion, we confirm the ability of LCP1 to drive metastatic behavior and correlate with poor outcomes in patients.
X -Ray Absorption Fine Structure (EXAFS and XANES) and Microscopic Investigations of Supramolecular Systems. (2001)

Self-assembled monolayers of n-alkanethiols, CH3-(CH 2)x-SH, on Au, Ag, and Cu have been studied with Grazing incidence EXAFS (GIXAFS) at the sulfur K-edge. Characterization of the bonding of self-assembled monolayers is of great interest because of their ability to tailor physical and chemical properties of surfaces. For both pentanethiol and decanethiol monolayers on Ag and Cu, the three-fold hollow site is found to be the most probable sulfur binding site. However, observations for octadecanethiol indicate that the three-fold-hollow site is not the exclusive binding site. In addition, the possible existence of disulfide bonds on the metal surface (adsorbed dialkyldisulfides) is not supported by the data. In addition, careful examination of the systems by XANES clearly demonstrate the peculiar behavior of octadecanethiol on surfaces. Preliminary results from monolayers on Au are also reported and constitute the first ever reported EXAFS data for alkanethiol monolayers on gold. These findings represent a great advance for the investigation of thin films on surfaces, and also present the possibility to study mixed monolayers as a complement to scanning probe microscopy. Recently, dendritic systems, and especially dendrimer-metal complexes, have been shown to have outstanding properties which make them suitable for applications in catalysis and biology. Our long-term plans include investigations of adsorbed dendrimer-metal complexes on metal surfaces through the use of grazing incidence EXAFS. Preliminary X-ray absorption studies of poly(propylene imine) dendrimer-copper(II) complexes of generation 1--5 in powder form and methanolic solution are presented here. XANES allows determination of the oxidation state of the copper species and the geometry of the copper complex whereas EXAFS gives us information about bond distances, coordination number and nature of the neighbor atoms. When reacted with NaBH4, these dendrimer-copper complexes undergo reduction which leads to the formation of Cu(0) nanoclusters. We investigated these clusters by EXAFS, XANES, ultra violet-visible spectroscopy and high resolution transmission electron microscopy (HR-TEM) and discuss their geometry, composition and size depending on the dendrimer generation. The synthesis of metallic nanoclusters by the dendrimer route has been successful to produce nanoclusters of reduced size and increased monodispersity.