gebhart/patrepeval huggingface dataset:
Synthesis, Characterization, and Luminescence Properties of the Ternary Europium Complex Covalently Bonded to Mesoporous SBA-15 (2005)

A novel mesoporous SBA-15 type of hybrid material (phen-SBA-15) covalently bonded with 1,10-phenanthroline (phen) ligand was synthesized by co-condensation of tetraethoxysilane (TEOS) and the chelate ligand 5-[N,N-bis-3-(triethoxysilyl)propyl]ureyl-1,10-phenanthroline (phen-Si) in the presence of Pluronic P123 surfactant as a template. The preservation of the chelate ligand structure during the hydrothermal synthesis and the surfactant extraction process was confirmed by Fourier transform infrared (FTIR) and (29)Si MAS NMR spectroscopies. SBA-15 consisting of the highly luminescent ternary complex Eu(TTA)(3)phen (TTA = 2-thenoyltrifluoroacetone) covalently bonded to a silica-based network, which was designated as Eu(TTA)(3)phen-SBA-15, was obtained by introducing the Eu(TTA)(3).2H(2)O complex into the hybrid materials via a ligand exchange reaction. XRD, TEM, and N(2) adsorption measurements were employed to characterize the mesostructure of Eu(TTA)(3)phen-SBA-15. For comparison, SBA-15 doped with Eu(TTA)(3).2H(2)O and Eu(TTA)(3)phen complexes and SBA-15 covalently bonded with a binary europium complex with phen ligand were also synthesized, and were named SBA-15/Eu(TTA)(3), SBA-15/Eu(TTA)(3)phen, and Eu-phen-SBA-15, respectively. The detailed luminescence studies on all the materials showed that, compared with the doping sample SBA-15/Eu(TTA)(3)phen and binary europium complex functionalized sample Eu-phen-SBA-15, the Eu(TTA)(3)phen-SBA-15 mesoporous hybrid material exhibited higher luminescence intensity and emission quantum efficiency. Thermogravimetric analysis on Eu(TTA)(3)phen-SBA-15 demonstrated that the thermal stability of the lanthanide complex was evidently improved as it was covalently bonded to the mesoporous SBA-15 matrix.
Molecular characterization of UV‐treated sugar beet somaclones using RFLP markers (1994)

Sugar beet plants regenerated from UV‐treated calluses were examined by restriction fragment length polymorphism (RFLP) analysis to determine the extent of somaclonal variation occurring at the DNA level. In total, 50 random sugar beet DNA sequences were used to screen 42 somaclones for genetic alterations. Three polymorphisms were detected among the 7 644 alleles analysed. From these data a mutation frequency of 0.03 ± 0.02% per allele was estimated. This frequency is in agreement with similar studies of somaclonal DNA variation using molecular markers and lies in the upper range of the spontaneous gene mutation frequencies found in plants. The two probegenotype combinations showing independent polymorphisms, were further analysed using the restriction enzymes Bam HI, Eco RI, Eco RV and Hind III. Both polymorphisms are likely to result from structural rearrangements rather than from point mutations. Differences in methylation among 10 of the investigated somaclones were tested for by comparing Hpa II and Msp I generated RFLP patterns. The somaclones showed extensive methylation, but no differences in their degree of methylation. Cytological analysis revealed 34 diploid, 8 tetraploid, but no aneuploid plants.
Hypertensive strains and normotensive 'control' strains. How closely are they related? (1992)

The spontaneously hypertensive rat and the Dahl salt-sensitive rat are the most widely studied genetic models of hypertension. Many investigators have attempted to study the pathogenesis of hypertension by comparing these strains with their respective normotensive "controls," the Wistar-Kyoto rat and the Dahl salt-resistant rat. However, the genetic relation between each of these hypertensive strains and its corresponding normotensive control has never been clearly defined. Based on an analysis of DNA "fingerprint" patterns generated with six multilocus probes, we found that the spontaneously hypertensive rat (Charles River Laboratories, Inc.) is genetically quite different from its normotensive Wistar-Kyoto control: these strains only share approximately 50% of their DNA fingerprint bands in common. The inbred Dahl salt-sensitive rat (SS/Jr strain) (Harlan Sprague Dawley, Inc.) and the Dahl salt-resistant rat (SR/Jr strain) share approximately 80% of their DNA fingerprint bands in common. To the extent that the genes identified by DNA fingerprint analysis are representative of loci dispersed throughout the rodent genome, the current findings provide evidence of extensive genetic polymorphism between these commonly used hypertensive strains and their corresponding normotensive controls, particularly in the spontaneously hypertensive rat model. These findings, together with the fact that an enormous number of biochemical and physiological differences have been reported between these hypertensive and normotensive strains, suggest that continued comparison of spontaneously hypertensive rats with Wistar-Kyoto rats or Dahl salt-sensitive with salt-resistant rats will have limited value for investigating the pathogenesis of hypertension.
Poly(ADP-ribose) polymerase in HeLa cells--a high resolution two-dimensional gel analysis. (1994)

PADPRP is an eukaryotic enzyme responsible for poly(ADP-ribosyl)ation of several chromatin-associated proteins. Consequently, the modified proteins acquire various lengths of negatively charged, covalently bound oligo or poly(ADP-ribosyl) homopolymers. The present study was undertaken to get an insight into the charge and size heterogeneity of the various auto-modified species of PADPRP and other protein acceptors of (ADP-ribose) polymers. Toward this end, we analyzed HeLa cells using high resolution two-dimensional gel electrophoresis (2D-PAGE). Resolution of HeLa total cellular lysates in the basic pH range combined with immunoblots and activity-blots revealed extensive modification and processing of the enzyme. In addition to the native enzyme, a protein of approximately 116 kDa, we observed several proteins that exhibit immunoreactivity to an antibody prepared against a peptide encompassing the N-terminal 20 amino acids of the polymerase. Several protein species showed auto-modifying potential in an in situ activity blot assay. We have also localized the ADP-ribosylated proteins in permeabilized HeLa cells to demonstrate protein acceptors of poly(ADP-ribose).
Three-Dimensional Cathodoluminescence by Focused Ion Beam - Scanning Electron Microscopy (2013)

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.
Controlled hydrolysis reactions of solvated [TiCln](4–n)+species. Crystal and molecular structures of [Mg(MeCN)6][{TiCl4(MeCN)}2(µ-O)]·4MeCN and [{TiCl2(MeCN)2(µ-O)}4]·2MeCN (1994)

Partial hydrolysis reactions of the solvated [TiCl5(MeCN)]– anion and of the adduct TiCl4·2MeCN gave the µ-oxo-dititanium anion [{TiCl4(MeCN)}2(µ-O)]2– and the neutral tetranuclear µ-oxo compound [{TiCl2(MeCN)2(µ-O)}4], respectively, as established by X-ray crystallographic studies. Crystals of [Mg(MeCN)6][{TiCl4(MeCN)}2(µ-O)]·4MeCN 1 are monoclinic, space group P21/n, a= 14.777(6), b= 14.603(6), c= 21.214(10)Å, β= 105.3(1)°. The structure contains discrete octahedral [Mg(MeCN)6]2+cations, [{TiCl4(MeCN)}2(µ-O)]2– anions and solvent (MeCN) molecules. Crystals of [{TiCl2(MeCN)2(µ-O)}4]·2MeCN 2 are orthorhombic, space group P212121a= 12.165(6), b= 12.367(6), c= 27.927(11)Å. The structure contains discrete [{TiCl2(MeCN)2(µ-O)}4] units and solvent (MeCN) molecules. In 1 both titanium atoms have an octahedral environment. The Ti–O–Ti bridge is approximately linear [174.7(3)°] with equivalent Ti–O distances of 1.783(4) and 1.764(4)Å. By contrast in 2 there are four Ti–O–Ti bridges but in each case the two Ti–O distances are significantly different with mean values of 1.668,1.954 Å.
Ionic liquid‐derived blood‐compatible composite membranes for kidney dialysis (2006)

A novel heparin- and cellulose-based biocomposite is fabricated by exploiting the enhanced dissolution of polysaccharides in room temperature ionic liquids (RTILs). This represents the first reported example of using a new class of solvents, RTILs, to fabricate blood-compatible biomaterials. Using this approach, it is possible to fabricate the biomaterials in any form, such as films or membranes, fibers (nanometer- or micron-sized), spheres (nanometer- or micron-sized), or any shape using templates. In this work, we have evaluated a membrane film of this composite. Surface morphological studies on this biocomposite film showed the uniformly distributed presence of heparin throughout the cellulose matrix. Activated partial thromboplastin time and thromboelastography demonstrate that this composite is superior to other existing heparinized biomaterials in preventing clot formation in human blood plasma and in human whole blood. Membranes made of these composites allow the passage of urea while retaining albumin, representing a promising blood-compatible biomaterial for renal dialysis, with a possibility of eliminating the systemic administration of heparin to the patients undergoing renal dialysis.
Thermal Modeling of Deposit Solidification in Uniform Droplet Spray Forming (1997)

In spray forming, the deposit thermal state is a key parameter which influences the microstructural evolution upon and after droplet impact onto the deposit. The uniform droplet spray (UDS) forming process has been developed to enable precise control of the droplet and deposit thermal state and the resultant material microstructure. By having a uniform droplet size throughout the spray, all the droplets deposited onto the substrate will have the same thermal state upon impact, allowing for precise control of the solidification process. This paper describes a one-dimensional, finite difference model that predicts the temperature and liquid fraction of the deposit during the UDS process. The model employs an explicit temperature-enthalpy method to incorporate a variety of solidification models. Experiments were conducted using Sn-15 wt percent Pb binary alloy. Temperatures were measured in the deposit and acceptable agreement with the simulation was obtained. Modeling has shown that the deposit thermal state is highly dependent on variations in spray conditions, which are predicted using droplet trajectory and droplet thermal models. Using the droplet and deposit models, the relationship between UDS process parameters and material microstructure can be understood.
Handheld Quantum Key Distribution (2018)

Using integrated optics enabled us to miniaturize QKD optics and to demonstrate handheld QKD. The robust device could be integrated with classical free space communication systems for links in metropolitan areas and to flying platforms.
Journal Bearings With Arbitrary Position of Source—II (1961)

A complete oil film solution of Reynolds equation is found that takes into account the presence of a point source. The solution gives integrated journal bearing characteristics in finite form. With the load in a vertical plane and the source at the top or bottom of the bearing, two simple universal relationships have emerged between the angle indicating the line of centers (α) and the eccentricity (b). With the source at the base of the bearing and −cos α = b, the plot of coefficient of friction versus the Sommerfeld variable goes through the origin and is essentially independent of bearing length to diameter ratio. Also, lubricant end flow becomes vanishingly small as the Sommerfeld variable approaches zero. With the source at the top of the bearing a friction axis intercept can be obtained depending upon a source parameter, q. With cos α ≈ b/2, a rapid rise in the co-efficient of friction can also be simulated by properly varying the source parameter. Also, lubricant end flow increases monotonically as the Sommerfeld variable approaches zero.
Performance of small-scale frequency references (2003)

We consider theoretically the expected performance of ultra-small vapor-cell frequency references with cell volumes below 1 mm/sup 3/. The short-term stability is found to degrade as the size is reduced. The amount of degradation depends on whether a buffer gas or wall coating is used for the atomic confinement. Preliminary experimental results with all-optical excitation indicate that the atomic Q-factor behaves as expected.
Understanding the harm from U.S. motor vehicle crashes (1995)

The personal and societal losses caused by motor vehicle crashes are significant. This paper provides tools which describe these losses for 30 different crash types. Persons involved with the development and implementation of crash countermeasures can use these tools to prioritize their countermeasure approach. Multiple vehicle crashes currently account for much larger direct costs but only slightly more years lost than single vehicle crashes. Direct expenditures on multiple vehicle crashes exceed $41 billion per year; they claim 974,000 years of life and functioning. Direct expenditures on single vehicle crashes exceed $18 billion per year; they claim 937,000 years of life and functioning.
Jasplakinolide Induces Apoptosis in Various Transformed Cell Lines by a Caspase-3-Like Protease-Dependent Pathway (2000)

To clarify the mechanisms underlying the antiproliferative effects of jasplakinolide, a cyclic depsipeptide from marine sponges, we examined whether jasplakinolide induces apoptosis in a variety of transformed and nontransformed cells. Jasplakinolide inhibited proliferation of human Jurkat T cells, resulting in cell death. This was accompanied by chromatin condensation and DNA cleavage at the linker regions between the nucleosomes. When caspase-3-like activity in the cytosolic extracts of Jurkat T cells was examined with a fluorescent substrate, DEVD-MAC (N-acetyl-Asp-Glu-Val-Asp-4-methyl-coumaryl-7-amide), the activity in the cells treated with jasplakinolide was remarkably increased in a time-dependent manner. Pretreatment of Jurkat T cells with the caspase inhibitor zVAD [benzyloxycarbonyl(Cbz)-Val-Ala-beta-Asp(OMe)-fluoromethylketone] or DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-1-aldehyde) prevented the induction of apoptosis by jasplakinolide. Moreover, exposure of various murine transformed cell lines to jasplakinolide resulted in cell death, which was inhibited by zVAD. Although it has been well established that murine immature thymocytes are sensitive to apoptosis when exposed to various apoptotic stimuli, these cells as well as mature T lymphocytes were resistant to jasplakinolide-induced apoptosis. The results suggest that jasplakinolide induces apoptotic cell death through a caspase-3-like protease-dependent pathway. Another important outcome is that transformed cell lines were more susceptible to jasplakinolide-induced apoptosis than normal nontransformed cells.
69-05: ARVC and myocarditis can coexist?Comparative study between CMR and EBM (2016)

Purpose of the Study: Recent studies noted and supposed the possible coexistence of arrhythmogenic right ventricular cardiomyopathy and myocarditis (ARVC) and myocarditis, considering the frequent finding of fibro-fatty infiltration and inflammatory component at histology. Cardiac magnetic resonance (CMR) is the only imaging modality able to depict fibro-fatty infiltration and fibrosis as late gadolinium enhancement (LGE) deposition. The endomiocardial biopsy (EBM) plays an important role in the definitive diagnosis of ARVC. The aim was to investigate by CMR and EBM the possible coexistence of two pathologies. Method Used: From 2010 to October 2015, 62 patients were enrolled and underwent a CMR. For each patient a right ventricular (RV) mapping was performed identifying the scar areas in the bipolar-unipolar maps. We obtained the right ventricular samples trough the right femoral vein via a disposable biotome . All the samples obtained were guided by the electroanatomical voltage mapping. Summary of Results: EBM was performed in 62 patients. In 7, 4 % (= 12) cases the biopsy was non diagnostic due to a sampling error. 3% (=6) aspecific. In the remaining 44 patients the biopsy showed the presence of adipose infiltration with fibrosis with a diagnosis of ARVC in 18 (7,9%) patient, in 10 (4,4%) an histological inflammatory infiltrate as a myocarditis. In 16 patients (7%) the biopsy shows both a fatty infiltration and an inflammatory component. In all cases of ARVC in the intraventricular septum and RV anterolateral wall LGE was noted. In none of patients, with a no istopatological diagnosis of ARVC was noted fibrofatty-infiltration at CMR. In all patients with a istopatological myocarditis the LGE pattern was midwall patchy. In remaining patients with a coexistence of ARVC and an inflammatory component the CMR presented only diagnostic criteria for ARVC, and no suspicion of myocarditis LGE pattern. Conclusions: In our experience in the most cases there is a concordance between the CMR and EBM in terms of scar areas-LGE and fibrosis in the istopathological finding. Despite the CMR was not to able with accuracy to discriminate the inflammatory component in cases of coexistence of two pathologies, many cases of ARVC could be present also a inflammatory component mimicking a myocarditis.
Circulating Tumor Cell Separation of Blood Cells and Sorting in novel Microfluidic approaches: a review (2020)

Separation and interpretation of rebellious Circulating Tumor Cells (CTCs) originating from the primary tumor or cancer tissue plays a significant role in diagnostics, cancer progression analyses, suitable medicine exploration, and treatment proficiency examination. Cancer metastasis occurs when CTCs spread throughout the body and invade healthy tissues, leading to new tumors in that area. Although a dramatic rate of deaths begins from spreading CTCs around the body, valuable measures have been made to control their development. However, the first step is separating these harmful cells from the bloodstream and investigating their features. Having examined the characteristics of CTCs as cancer’s main strength, researchers can introduce complementary treatments that can affect cancerous cells without damaging the healthy cells. Therefore, according to their unique characteristics, numerous techniques have been established for continuous and fast separation and sorting of CTCs. Nevertheless, few separators enjoy the efficient performance and appropriate accuracy and can be produced in mass numbers due to the available fabrication equipment. Microfabrication advancements enable separators to combine the advantages of active and passive methods in a small-scale platform for probing individual cells and separation purposes. Reduction in reagents, sample volume, analysis time, and less harmfulness to patients are some of the motivations that encourage researchers to employ microfluidic instruments for CTCs separation from other blood cells over the last two decades. However, microfabrication limitations mean effective separators, and the diagnostic option they provide, are not readily available. Addressing these limitations requires optimizing the design and fabrication of separators such that they are reduced in their size and fabrication cost, while also maintaining high-throughput separating capability. The emergence of the Lab-On-a-Chip (LOC) and then Lab-On-a-CD (LOCD) technologies, having more inherent benefits than conventional microfluidic devices, has created new opportunities and become increasingly widespread in recent years. Evidence suggests that employing single methodologies or integrating approaches without sufficient understanding of potential outcomes is unlikely to result in successful diagnostic results. This paper contributes an extensive review of several separation systems, including fundamental theories and experimental details, and describes detailed operating principles and device performance.
Pooled CRISPR screening with single-cell transcriptome read-out (2016)

Abstract CRISPR-based genetic screens have revolutionized the search for new gene functions and biological mechanisms. However, widely used pooled screens are limited to simple read-outs of cell proliferation or the production of a selectable marker protein. Arrayed screens allow for more complex molecular read-outs such as transcriptome profiling, but they provide much lower throughput. Here we demonstrate CRISPR genome editing together with single-cell RNA sequencing as a new screening paradigm that combines key advantages of pooled and arrayed screens. This approach allowed us to link guide-RNA expression to the associated transcriptome responses in thousands of single cells using a straightforward and broadly applicable screening workflow.
Decentralized output-feedback stabilization for interconnected discrete systems with unknown delays (2009)

The decentralized feedback stabilization problem of a class of nonlinear interconnected discrete-time systems is considered. This class of systems has unknown-but-bounded state-delay and uncertain nonlinear perturbations satisfying quadratic constraints that are functions of the overall state and delayed state vectors. A decentralized output feedback scheme is proposed and analyzed such that the overall closed-loop system guarantees global delay-dependent stability condition, derived in terms of local subsystem variables. Incorporating feedback gain perturbations, new resilient decentralized feedback scheme is subsequently developed. The proposed approach is formulated within the framework of convex optimization over linear matrix inequalities. Simulation results illustrate the effectiveness of the proposed decentralized output-feedback controllers. Copyright © 2009 John Wiley & Sons, Ltd.
High Pressure Micronization of Tristearate (2009)

Abstract The effect of the pre‐expansion temperature, pre‐expansion pressure and storage time was studied in order to investigate the crystal form, degree of crystallinity, particle size, particle size distribution and morphology of the tristearate. The experiments were performed at pressures between 115 and 215 bar and at temperatures of 54, 60 and 70 °C with the substance being already melted. Samples were characterized immediately after the experiments and later after 3 months of storage at 5 and 20 °C. Results showed an increase in the amorphous phase. During the storage, the micronized samples re‐crystallized. With time, the agglomeration was more emphasized for particles produced at higher temperatures and pressures and stored at a higher temperature. The results of X‐ray analysis did not show a change in crystal form of tristearate (β′ form) before and after micronization, however, for samples stored at 20 °C slow transformation from the β′ (less stable) form to the β (more stable) form occurred. In addition, the morphology of micronized samples showed an irregular and porous shape. The re‐crystallization was more evident with time for samples stored at 20 °C where needle‐like crystals appeared on the surface.
Scientific and Engineering Computing Using ATI Stream Technology (2009)

This continuing exploration of GPU technology examines ATI Stream technology and its use in scientific and engineering applications.
Interpolation formulas for the local field factors (2005)

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