gebhart/patrepeval huggingface dataset:
Decoy receptor 3 promotes cell adhesion and enhances endometriosis development (2017)

Endometriosis is a multifactorial inflammatory disease with persistent activation of the nuclear factor-κB (NF-κB) signalling pathway. Aberrant adhesion of endometrium is the essential step in the progression of endometriosis, but the molecular mechanism of ectopic growth of endometrium is still unclear. Decoy receptor 3 (DcR3)/TNFRSF6B, a pleiotropic immunomodulator regulated by oestrogen, is able to activate focal adhesion kinase to promote cell adhesion. We found that DcR3 is upregulated in human ectopic endometrial cells via activation of the Akt-NF-κB signalling pathway, and its expression level correlates positively with that of the adhesion molecules intercellular adhesion molecule 1 (ICAM-1) and homing cell adhesion molecule (HCAM; CD44). In a multivariate regression model, DcR3 expression level was the most significant parameter associated with endometriosis severity. Knockdown of DcR3 not only downregulated the expression of ICAM-1 and HCAM, but also reduced cell adhesion and migration. In vivo investigation further showed that DcR3 promoted the growth and spread of endometrium, whereas knockdown of DcR3 by lentivirus-delivered short hairpin RNA inhibited ectopic adhesion of endometrium and abrogated endometriosis progression. These observations are in support of DcR3 playing a critical role in the pathogenesis of endometriosis, and the inhibition of DcR3 expression being a promising approach for the treatment of endometriosis. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Le travail du cuir à Troyes de l’époque romaine au XXe siècle. Etude technico-typologique du matériel mis au jour sur le site de l’Hôtel du Département : premiers résultats et orientation de la recherche (2014)

International audience
Smoking at diagnosis and survival in cancer patients (2012)

The effect of smoking on survival in cancer patients is limited by the lack of structured prospective assessments of smoking at diagnosis. To assess the effect of smoking at diagnosis on survival, structured smoking assessments were obtained in a cohort of 5,185 cancer patients within 30 days of a cancer diagnosis between 1982 and 1998. Hazard ratios (HRs) or odds ratios were generated to analyze the effects of smoking at diagnosis on overall mortality (OM) and disease-specific mortality (DSM) in a patient cohort from 13 disease sites containing at least 100 patients in each disease site. With a minimum of 12 years of follow-up, current smoking increased OM risk versus recent quit (HR 1.17), former (HR 1.29) and never smokers (HR 1.38) in the overall cohort. Current smoking increased DSM risk versus former (HR 1.23) and never smokers (HR 1.18). In disease sites with proportionately large (>20%) recent quit cohorts (lung and head/neck), current smoking increased OM and DSM risks as compared with recent quit. Current smoking increased mortality risks in lung, head/neck, prostate and leukemia in men and breast, ovary, uterus and melanoma in women. Current smoking was not associated with any survival benefit in any disease site. Data using prospective structured smoking assessments demonstrate that current smoking increased long-term OM and DSM. Standardized smoking assessment at diagnosis is an important variable for evaluating outcomes in cancer patients.
Albumin-bound fluorescence in serum of patients with chronic renal failure. (1980)

A band that is strongly fluorescent and migrates electrophoretically with serum albumin is commonly found in electrophoretograms of sera from patients with chronic renal failure. We sought to determine whether the fluorescence originates from binding of certain still-unidentified metabolites or drugs, from an abnormal albumin species, or from some other protein entity. Molecular-exclusion column chromatography, polyacrylamide gel isoelectric focusing, and cellulose acetate electrophoresis, along with results of charcoal treatment and alcohol extraction, provided evidence that the fluorescence comes from fluorescent ligands tightly bound to albumin. The fluorescent intensity of the albumin fraction, isolated by molecular-exclusion chromatography, coincides with the albumin-associated fluorescence determined electrophoretically and with the intensities of the fluorescence emission spectrum for serum. A fluorescent species with an emission maximum of 415 +/- 5 nm, separated by thin-layer chromatography, appears to account for the increased serum fluorescence.
Effect of storage conditions on handling and SO2 reactivity of CA(OH)2-based sorbents (1991)

The article gives results of an investigation of the effect of relative humidity (RH), time, and aeration during calcium hydroxide--Ca(OH)2--storage for its effect on sorbent handling and reactivity with sulfur dioxide (SO2). Investigated was the effect of sorbent storage conditions of time (1-24 hr), RH (zero-90%), silo wall material, and aeration on handling properties of flowability and floodability and their subsequent effect on sorbent/SO2 reactivity. Increased RH in the storage chamber and prolonged storage increased floodability, as predicted by the angle of difference. No significant effect of RH on the flowability of Ca(OH)2, as predicted by the angle of repose, was detected. The importance of silo wall material on proper sorbent discharge pattern has been demonstrated through testing on four common surfaces. The effect of sorbent storage conditions on the reactivity of Ca(OH)2 with SO2 was evaluated in a short time differential reactor (STDR)operated under conditions typical of dry sorbent injection for SO2 control near the preheater. Increased RH and aeration with air during storage resulted in decreased reactivity of Ca(OH)2 with SO2. The effect of storage conditions on handling of novel Ca(OH)2-based sorbents for the removal of SO2 was also evaluated. ADVACATE sorbent appears to have significantly better handlingmore » properties than the other sorbents tested.« less
Chromatin mapping and single-cell immune profiling define the temporal dynamics of ibrutinib response in CLL (2020)

The Bruton tyrosine kinase (BTK) inhibitor ibrutinib provides effective treatment for patients with chronic lymphocytic leukemia (CLL), despite extensive heterogeneity in this disease. To define the underlining regulatory dynamics, we analyze high-resolution time courses of ibrutinib treatment in patients with CLL, combining immune-phenotyping, single-cell transcriptome profiling, and chromatin mapping. We identify a consistent regulatory program starting with a sharp decrease of NF-κB binding in CLL cells, which is followed by reduced activity of lineage-defining transcription factors, erosion of CLL cell identity, and acquisition of a quiescence-like gene signature. We observe patient-to-patient variation in the speed of execution of this program, which we exploit to predict patient-specific dynamics in the response to ibrutinib based on the pre-treatment patient samples. In aggregate, our study describes time-dependent cellular, molecular, and regulatory effects for therapeutic inhibition of B cell receptor signaling in CLL, and it establishes a broadly applicable method for epigenome/transcriptome-based treatment monitoring.
Separation of Organic Dyes from Wastewater by Using Colloidal Gas Aphrons (1992)

Abstract Auxiliary chemicals used in the preparation of water-based dyes have been reported to pose problems in conventional wastewater treatment processes. The dye manufacturing industries have to find alternate wastewater treatment processes to remove dyes from their waste effluents. This paper investigates a novel method of separation of dyes by flotation using colloidal gas aphrons (CGAs). The results indicate that this is an effective method for the separation of synthetic dyes from wastewater. The mechanism of removal has been shown to be ion coupling of the oppositely charged species of the surfactant forming the CGA and the dye and flotation of the ion-dye complex on the surface of CGA microbubbles.
Measurement Method for Organic Halogens and its Application to Flue Gas Monitoring (2008)

The persistent and hazardous nature of some legally regulated dioxins, including PCDD/Fs and dioxin-like PCBs, makes them of particular concern. However, it is currently not possible to estimate the potential risk of exhaust gases and to act on dioxin measurement data taken in real time at incineration plants, since obtaining results from samples takes time. This study investigates the potential for real-time measurement of organic halogens using atomic emission spectrometry employing radio-frequency helium plasma. Investigations were conducted on the adsorption characteristics of various graphite-based dioxin adsorbents, the basic dose-response relationships of this detection system, and actual gas sampling/measurement using a continuous gas sampling apparatus. The following results were obtained. i)The relationship between halogen amount ratio and peak area ratio using chlorobenzene and bromobenzene as standard compounds showed good linearity. The coefficients between the two parameters varied from 0.66 to 1.4, averaging 1.0. ii)The Carbotrap C tube collected compounds with a boiling point above 180°C; addition of a Carbopak X tube made it possible to collect a wide range of gaseous organic halogens. The gas sampling tube was incorporated into a semi-continuous sampling apparatus and used for flue gas sampling at actual incineration plants. The sum of the concentrations of organic chlorines and bromines during two five-day measurement periods varied from 0.61 to 9.4μg/m3. This rapid and easy measurement method of organic halogens thus has considerable potential for the management of hazardous substances at incineration plants.
Purification and properties of oestradiol 17β-dehydrogenase extracted from cytoplasmic vesicles of porcine endometrial cells (1992)

Porcine endometrial oestradiol-17 beta dehydrogenase was solubilized from the particulate fraction of homogenates sedimenting between 1200 g and 10,000 g by treatment with 0.4% Brij 35 in neutral buffers. The extracts were processed by successive passage through DEAE-Sepharose, Amberlyte XAD-2 and Blue-Sepharose, and the enzyme was collected from the washed affinity matrix at 0.8 M of a 0-2 M-KCl gradient. A genuine oestrone reductase was eluted at 1.9 M-KCl. The dehydrogenase pool was resolved by butyl-Sepharose chromatography into a major (80%) peak (EDHM) eluted at 0.8 M-(NH4)2SO4 and a very hydrophobic fraction (VHF) recovered at 0.1 M. EDHM was further purified by filtration through Sephadex G-200 and cation-exchange chromatography on Mono S. Sephacryl 300 was used for VHF followed by Mono S. Enrichments from the homogenate amounted to 1074-fold for EDHM and 632-fold for VHF. A single silver-stained band at 32 kDa is seen on SDS/PAGE of EDHM, and VHF contains additional bands at 45 and 80 kDa. Polyclonal antibodies (G436) raised against EDHM and the monoclonal antibody F1 raised against VHF recognize the single 32 kDa band in EDHM and both the 32 kDa and 80 kDa bands in composite VHF. The 45 kDa band of VHF reacts with neither. Monoclonal antibody W1 raised against EDHM only recognizes the 32 kDa peptide of EDHM and VHF. The specific activity for oestradiol oxidation amounts to 4081 mu-units/mg for EDHM and to 2402 mu-units/mg for VHF. Both possess a minimal (1/260) endogenous reductase activity and are devoid of 3 beta, 3 alpha- and 20 alpha-dehydrogenases. We consider EDHM to be authentic oestradiol-17 beta dehydrogenase of porcine endometrium. The composite VHF could reflect the situation of the enzyme in vivo or result from aggregations occurring during processing.
Effects of protein tyrosine kinase and protein tyrosine phosphatase on apical K+channels in the TAL (2001)

We have previously demonstrated that the protein level of c-Src, a nonreceptor type of protein tyrosine kinase (PTK), was higher in the renal medulla from rats on a K-deficient (KD) diet than that in rats on a high-K (HK) diet (Wang WH, Lerea KM, Chan M, and Giebisch G. Am J Physiol Renal Physiol 278: F165-F171, 2000). We have now used the patch-clamp technique to investigate the role of PTK in regulating the apical K channels in the medullary thick ascending limb (mTAL) of the rat kidney. Inhibition of PTK with herbimycin A increased NP(o), a product of channel number (N) and open probability (P(o)), of the 70-pS K channel from 0.12 to 0.42 in the mTAL only from rats on a KD diet but had no significant effect in tubules from animals on a HK diet. In contrast, herbimycin A did not affect the activity of the 30-pS K channel in the mTAL from rats on a KD diet. Moreover, addition of N-methylsulfonyl-12,12-dibromododec-11-enamide, an agent that inhibits the cytochrome P-450-dependent production of 20-hydroxyeicosatetraenoic acid, further increased NP(o) of the 70-pS K channel in the presence of herbimycin A. Furthermore, Western blot detected the presence of PTP-1D, a membrane-associated protein tyrosine phosphatase (PTP), in the renal outer medulla. Inhibition of PTP with phenylarsine oxide (PAO) decreased NP(o) of the 70-pS K channel in the mTAL from rats on a HK diet. However, PAO did not inhibit the activity of the 30-pS K channel in the mTAL. The effect of PAO on the 70-pS K channel was due to indirectly stimulating PTK because pretreatment of the mTAL with herbimycin A abolished the inhibitory effect of PAO. Finally, addition of exogenous c-Src reversibly blocked the activity of the 70-pS K channel in inside-out patches. We conclude that PTK and PTP have no effect on the low-conductance K channels in the mTAL and that PTK-induced tyrosine phosphorylation inhibits, whereas PTP-induced tyrosine dephosphorylation stimulates, the apical 70-pS K channel in the mTAL.
Voltage Rollercoaster Filtering of Low-Mass Contaminants During Native Protein Analysis (2020)

Intact protein mass spectrometry (MS) via electrospray-based methods is often degraded by low-mass contaminants, which can suppress the spectral quality of the analyte of interest via space-charge effects. Consequently, selective removal of contaminants by their mobilities would benefit native MS if achieved without additional hardware and before the mass analyzer regions used for selection, analyte readout, or tandem MS. Here, we use the high-pressure multipole within the source of an Orbitrap Tribrid as the foundation for a coarse ion filter. Using this method, we show <i>complete</i> filtration of 2 mM polyethylene glycol (PEG-1000) during native MS of SILu mAb antibody present at a 200× lower concentration. We also show the generality of the process by rescuing 10 μM tetrameric pyruvate kinase from 2 mM PEG-1000, asserting this voltage rollercoaster filtering (VRF) method for use in native MS as an efficient alternative to conventional purification methods.
ChemInform Abstract: Site‐Specific 15N‐Labeling of Adenine in DNA for NMR Studies. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Fine-Mapping and Identification of a Candidate Gene Underlying thed2Dwarfing Phenotype in Pearl Millet,Cenchrus americanus(L.) Morrone (2013)

Pearl millet is one of the most important subsistence crops grown in India and sub-Saharan Africa. In many cereal crops, reduced height is a key trait for enhancing yield, and dwarf mutants have been extensively used in breeding to reduce yield loss due to lodging under intense management. In pearl millet, the recessive d2 dwarfing gene has been deployed widely in commercial germplasm grown in India, the United States, and Australia. Despite its importance, very little research has gone into determining the identity of the d2 gene. We used comparative information, genetic mapping in two F2 populations representing a total of some 1500 progeny, and haplotype analysis of three tall and three dwarf inbred lines to delineate the d2 region by two genetic markers that, in sorghum, define a region of 410 kb with 40 annotated genes. One of the sorghum genes annotated within this region is ABCB1, which encodes a P-glycoprotein involved in auxin transport. This gene had previously been shown to underlie the economically important dw3 dwarf mutation in sorghum. The cosegregation of ABCB1 with the d2 phenotype, its differential expression in the tall inbred ICMP 451 and the dwarf inbred Tift 23DB, and the similar phenotype of stacked lower internodes in the sorghum dw3 and pearl millet d2 mutants suggest that ABCB1 is a likely candidate for d2.
Shape Memory Alloy Cables: Exploratory Experiments (2009)

Conventional structural cables (or wire ropes) are composed of steel wires helically wound into strands, which, in turn, are wound around a core. Cables made from NiTi shape memory alloy (SMA) wires are a new structural element with promising adaptive and enhanced structural properties for a broad range of applications. Potential advantages of this form include increased bending flexibility for spooling/packaging, better fatigue performance, energy absorption and damping, reduced thermal lag, redundancy, and significant design flexibility. Exploratory thermomechanical experiments were performed on a conventional cable construction: the right regular lay 7 × 7, consisting of 7 strands with 7 wires per strand. Uniaxial tension experiments characterize the cables’ sensitivity to strain rate, temperature, and lubrication. Experiments were also performed on individual strands and wires from the cable to study the hierarchical nature of the cable construction. Special attention was paid to the propagation of phase transformation fronts, similar to that seen previously in uniaxial tension of NiTi wire.
Hydroxyl‐terminated poly(urethane acrylate) as a soft liner in dental applications: Synthesis and characterization (2010)

Abstract Hydroxyl‐terminated poly(urethane acrylate)s were synthesized for use in biomedical applications. Acrylate end capping via an interesterification reaction was successfully achieved with methacryloyl chloride addition to the hydroxyl ends of the polyurethane at low temperatures. 2,4‐Toluene diisocyanate, 1,6‐hexane diisocyanate, and methylene diphenyl diisocyanate were used as diisocyanates for urethane synthesis, and they were end‐capped with methyl methacrylate and hydroxyethyl methacrylate. The nature of the monomers that we used had an effect on the thermal and morphological properties that were interpreted in terms of the level of hydrogen bonding and the degree of phase separation. The synthesized polymers were characterized by NMR, Fourier transform infrared/attenuated total reflectance spectroscopy, differential scanning calorimetry, thermogravimetric analysis, and gel permeation chromatography. The number‐average molecular weights of the poly(urethane acrylate)s were 2500–6000 g/mol. To use the polymer as a soft‐liner material in denture applications, the residual isocyanate should not exist. In this study, we showed that a prepolymer without residual isocyanate could be synthesized. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Drilled dielectric membranes for highly resonant filtering in the infrared (2007)

Subwavelength dielectric and metallic gratings embedded in vacuum can act as highly-resonant spectral filters. We review the theoretical principles for the design of symmetric dielectric and metal gratings to conceive efficient optical filters in the mid and far infrared range, and we show how both resonance width and resonance wavelength can be tuned. We describe an original process for the fabrication of free-standing SiC gratings, and we present the first samples obtained with 10 &mgr;m period. Experimental angularly resolved transmission spectra show evidences of their filtering properties.
Selective Detection of the Carbohydrate-Bound State of Concanvalin A at the Single Molecule Level (1997)

The labeling of molecules with charge-transfer dyes, such as 5-(dimethylamino)-1-napthalenesulfonyl (dansyl) chloride, is a powerful tool for examining the solvent shell of attached substances. This investigation describes the synthesis and application of a new charge transfer label, based on trans-1-[p-(N,N-dimethylamino)phenyl]-trans-4-(p-nitrophenyl)-1,3-butadiene (NND). Unlike many commonly used fluorophores, the quantum yield of NND decreases over 4 orders of magnitude upon changing from nonpolar to polar environments. In addition, several derivatives of NND undergo little photodecomposition and can be detected at the picomolar level in a confocal fluorescence correlation spectrometer. In conjunction with recent detection of single molecules in solution, this paper describes a method to discriminate between single free and carbohydrate-bound aggregates of the Jack Bean lectin, concanavalin A (Con A). To this end, two derivatives of NND were constructed possessing an additional functional handle. One derivative, alkenyne 4, was efficiently attached to the β-anomeric position of glucopyranosides. Transients from single aggregates of this fluorophore were detected in solutions which contained both Con A and maltoside 1, and not the corresponding glucoside 2. This result is in agreement with the known affinity of Con A for α-glucopyranosides and not β-glucopyranosides. A full description of the synthesis of these dyes, their solvochromatic properties, and the method used for single aggregate detection is provided herein.
The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb. (1995)

Rak is a nuclear tyrosine kinase containing Src homology 2 and 3 domains at its NH2 terminus. We report here that the retinoblastoma tumor susceptibility gene product pRb associates with Rak in vivo and in vitro. Rak binds in the A/B pocket region of pRb, a region that is frequently mutated in human cancer, during the G1 and S phases of the cell cycle. Furthermore, Rak expression is elevated in G1, and transfection of Rak into NIH 3T3 cells results in a significant decrease in the number of emerging colonies. Thus, Rak is a tyrosine kinase with growth suppressing activity that may function, in part, through its interaction with pRb.
The Mechanism of Enzymatic Cellulose Degradation (1976)

1. A low-molecular-weight and a high-molecular-weight 1,4beta-glucan glucanohydrolase (Cx enzyme) have been isolated from a commercial cellulase preparation derived from culture filtrates of the fungus Trichoderma viride. 2. The purification method for the isolation of the low-molecular-weight enzyme is a three-step procedure including chromatography on Bio-Gel P-10, chromatography on a dipolar adsorbent (arginine-Sepharose 6 B) and isoelectric focusing. 3. The starting material for the isolation of the high-molecular-weight enzyme was pre-fractionated by chromatography on Bio-Gel P-10, by DEAE-Sephadex chromatography and by SE-Sephadex chromatography as described previously by us. Further fractionation of this material was achieved by affinity chromatography and repeated isoelectric focusing. 4. Free zone electrophoresis of the low-molecular-weight enzyme indicated a homogeneous protein. The high-molecular-weight enzyme was homogenous in sedimentation equilibrium analysis. 5. The molecular weights of the enzymes were 12 500 and 50 000 +/- 2000 respectively. The former value was determined by chromatography on a calibrated column of Bio-Gel P-100 and the latter value by sedimentation equilibrium analysis. 6. The low-molecular-weight enzyme was isoelectric at pH 4.60 (10 degrees C) and contained 21% carbohydrate. The corresponding values for the high-molecular-weight enzyme were pH 3.39 and 12%. 7. Both enzymes were active in releasing free fibers from filter-paper. The low-molecular-weight enzyme was estimated to be about twice as effective as the high-molecular-weight enzyme in this regard.