gebhart/patrepeval huggingface dataset:
Overexpression of miR-146a in basal-like breast cancer cells confers enhanced tumorigenic potential in association with altered p53 status (2014)

The tumor suppressor p53 is the most frequently mutated gene in human cancers, mutated in 25-30% of breast cancers. However, mutation rates differ according to breast cancer subtype, being more prevalent in aggressive estrogen receptor-negative tumors and basal-like and HER2-amplified subtypes. This heterogeneity suggests that p53 may function differently across breast cancer subtypes. We used RNAi-mediated p53 knockdown (KD) and antagomir-mediated KD of microRNAs to study how gene expression and cellular response to p53 loss differ in luminal versus basal-like breast cancer. As expected, p53 loss caused downregulation of established p53 targets (e.g. p21 and miR-34 family) and increased proliferation in both luminal and basal-like cell lines. However, some p53-dependent changes were subtype specific, including expression of miR-134, miR-146a and miR-181b. To study the cellular response to miR-146a upregulation in p53-impaired basal-like lines, antagomir KD of miR-146a was performed. KD of miR-146a caused decreased proliferation and increased apoptosis, effectively ablating the effects of p53 loss. Furthermore, we found that miR-146a upregulation decreased NF-κB expression and downregulated the NF-κB-dependent extrinsic apoptotic pathway (including tumor necrosis factor, FADD and TRADD) and antagomir-mediated miR-146a KD restored expression of these components, suggesting a plausible mechanism for miR-146a-dependent cellular responses. These findings are relevant to human basal-like tumor progression in vivo, since miR-146a is highly expressed in p53 mutant basal-like breast cancers. These findings suggest that targeting miR-146a expression may have value for altering the aggressiveness of p53 mutant basal-like tumors.
Adaptive Voltage Scaling via Effective On-Chip Timing Uncertainty Measurements (2012)

National audience
Establishment of an In Vivo Report System for the Evaluation of Amber Suppression Activity in Escherichia coli (2009)

Site-specific incorporation of unnatural amino acids into proteins in vivo can be achieved by co-expression of an orthogonal pair of suppressor tRNA and engineered aminoacyl-tRNA synthetase (ARS) that specifically ligates an unnatural amino acid to the suppressor tRNA. As a step to establish this technique, here we generated an Escherichia coli reporter strain DH10B(Tn:lacZam) by integrating amber mutated lacZ gene into the chromosome of E. coli DH10B strain. In vivo expression of E. coli amber suppressor produced blue colonies in culture plates containing X-Gal as well as dramatically increased -galactosidase activity. In addition, expression of an orthogonal pair of Saccharomyces cerevisiae suppressor and tyrosyl-tRNA synthetase also produced blue colonies as well as moderate increase of -galactosidase activity. These data demonstrate that our reporter strain will provide an efficient method to assess amber suppression in both qualitative and quantitative manners.
Development of Surface Reaction Mechanisms of CO / O2 on Pt and Rh for Three Way Catalyst based on Gas Phase and Surface Species Analyses (2020)

In this study, detailed surface reaction mechanisms for CO/O2 system on Pt/Al2O3 and Rh/Al2O3 have been developed based on the measurements of gaseous and surface species. The conventional conversion rate experiments for gaseous CO to CO2 were conducted with the monolith honeycomb catalyst, and furthermore, the adsorbed surface species were identified with the powdered catalyst by in-situ FTIR under the same conditions as conversion rate experiment. As a result, it was found that only linear CO adsorption was detected for Pt, while twin CO adsorption was detected for Rh. Based on the results, detailed surface reaction mechanisms have been developed for Pt/Al2O3 and Rh/Al2O3. As a result of numerical simulation, it was confirmed that the variations of CO to CO2 conversion rate with temperature were quantitatively reproduced for various O2 concentration conditions. Furthermore, using those reaction mechanisms, CO conversion rate on bimetal, Pt/Rh Al2O3 catalysts were examined for several Pt/Rh ratios. Numerical simulation results for bimetal catalysts were also quantitatively agreed with experimental results for various Pt/Rh ratios as well as CO/O2 ratios.
The argon laser in surgery for chronic ear disease and otosclerosis (1983)

The use of the argon laser in chronic ear disease and otosclerosis surgery is reviewed. Its advantages and limitations are discussed, and safe parameters for use established. The argon laser is an effective tool to remove, cut, and coagulate tissue. It was used in over 500 microsurgical otologic procedures without deleterious effects. One hundred primary laser stapedotomies were performed, and the audiological results were compared to those obtained using conventional small fenestra stapedectomy techniques. No significant statistical difference was present in the audiologic results after 1 year of observation. The clinical course of the laser stapedotomy patients suggested this technique was less traumatic than conventional techniques.
Optical frequency shifting of a mode-locked laser beam (1968)

An optical frequency shifter making use of the electro-optic effect in LiNbO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> was built to shift the Fourier spectrum of a mode-locked He-Ne laser; the shift was continuously adjustable from -45 GHz to +45 GHz about the central optical frequency of 473.61 THz. Shifted Fourier spectra recorded with a scanning Fabry-Perot interferometer were found to be in excellent agreement with theory.
Cholesteryl ester hydrolase activity in human symptomatic atherosclerosis (1980)

Acid cholesteryl ester hydrolase (CEH) activity was assayed in mononuclear cells of patients with symptomatic atherosclerosis (transient ischemic attacks, TIA) and in age-matched controls showing no evidence of atherosclerosis. The acid CEH level of TIA patients was significantly lower than that of controls (1074 +/- 128 vs 2113 +/- 255 pmol/mg P/hr, mean +/- SE). Neither mononuclear cell nor plasma cholesterol and cholesteryl ester concentrations differed significantly between atherosclerotic and control groups. TIA women had lower mononuclear cell concentrations of free cholesterol than men.
Terahertz plasmonic high pass filter (2003)

Metamaterials, which contain engineered subwavelength microstructures, can be designed to have positive or negative ε and μ at desired frequencies. In this letter, we demonstrate a metamaterial which has a “plasmonic” response to electromagnetic waves in the terahertz (THz) range. The sharp change of reflection and transmission at this plasma frequency makes the structure a high pass filter. The reflection response is characterized by Fourier transform infrared spectroscopy, and a plasma frequency at 0.7 THz is observed, which agrees with the theoretical calculation. The metamaterial is a two-dimensional cubic lattice consisting of thin metal wires, having wire diameter of 30 μm, lattice constant of 120 μm, and wire length of 1 mm. The microstereolithography technique is employed to fabricate the high-aspect-ratio lattice.
Isolation and Identification of Acaricidal Compounds in Inula japonica (Asteraceae) (2011)

We identified acaricidal compounds in Inula japonica Thunberg (Asteraceae) that were effective against carmine spider mite, Tetranychus cinnabarinus (Boisduval). Petroleum-ether extracts from I. japonica were toxic to T. cinnabarinus, with a 50% lethal concentration (LC50) value of 1.18 mg/ml. Silica gel column chromatography was used to separate the acaricidal components. Seventeen of 38 fraction groups had mite mortality rates >50%, nine fraction groups had rates >60%, and three fraction groups had rates >80% at 2 mg/ml. The major volatile compounds in the bioactive fraction groups were identified by gas chromatography-mass spectroscopy, and beta-sitosterol (1), stigmasterol (2), lupeol (3), and alpha-amyrin (4) were identified and determined to have acaricidal activity against T. cinnabarinus in vitro.
Influence of sintering parameters of aluminum titanate (TiAl2O5) on microstructure and on thermomechanical properties (2015)

International audience
Histopathological Breast Cancer Image Classification by Deep Neural Network Techniques Guided by Local Clustering (2018)

Breast Cancer is a serious threat and one of the largest causes of death of women throughout the world. The identification of cancer largely depends on digital biomedical photography analysis such as histopathological images by doctors and physicians. Analyzing histopathological images is a nontrivial task, and decisions from investigation of these kinds of images always require specialised knowledge. However, Computer Aided Diagnosis (CAD) techniques can help the doctor make more reliable decisions. The state-of-the-art Deep Neural Network (DNN) has been recently introduced for biomedical image analysis. Normally each image contains structural and statistical information. This paper classifies a set of biomedical breast cancer images (BreakHis dataset) using novel DNN techniques guided by structural and statistical information derived from the images. Specifically a Convolutional Neural Network (CNN), a Long-Short-Term-Memory (LSTM), and a combination of CNN and LSTM are proposed for breast cancer image classification. Softmax and Support Vector Machine (SVM) layers have been used for the decision-making stage after extracting features utilising the proposed novel DNN models. In this experiment the best Accuracy value of 91.00% is achieved on the 200x dataset, the best Precision value 96.00% is achieved on the 40x dataset, and the best <i>F</i>-Measure value is achieved on both the 40x and 100x datasets.
Synthesis of Pharmaceutically Relevant 2‐Aminotetralin and 3‐Aminochroman Derivatives via Enzymatic Reductive Amination (2021)

2-Aminotetralin and 3-aminochroman derivatives are key structural motifs present in a wide range of pharmaceutically important molecules. Herein, we report an effective biocatalytic approach towards these molecules through the enantioselective reductive coupling of 2-tetralones and 3-chromanones with a diverse range of primary amine partners. Metagenomic imine reductases (IREDs) were employed as the biocatalysts, obtaining high yields and enantiocomplementary selectivity for >15 examples at preparative scale, including the precursors to Ebalzotan, Robalzotan, Alnespirone and 5-OH-DPAT. We also present a convergent chemo-enzymatic total synthesis of the Parkinson's disease therapy Rotigotine in 63 % overall yield and 92 % ee.
Global gene expression profiling ofEhrlichia ruminantiumat different stages of development (2011)

Ehrlichia ruminantium (ER), the causative agent of heartwater on ruminants, is an obligate intracellular bacterium transmitted by ticks of the genus Amblyomma. Previous studies have shown that early stages of development may be critical for Ehrlichia pathogenicity. To gain insights into the biology of intracellular ER, we determined the genome-wide transcriptional profile of ER replicating inside bovine aortic endothelial cells using DNA microarrays. At intermediate and late stages of infection (reticulate and elementary bodies, respectively), a total of 54 genes were differentially expressed. Among them, we measured by q-RTPCR the overexpression of 11 of 14 genes. A number of genes involved in metabolism, nutrient exchange, and defense mechanisms, including those involved in resistance to oxidative stress, were significantly induced in ER reticulate bodies. This is consistent with the oxidative stress condition and nutrient starvation that seem to occur in Ehrlichia-containing vacuoles. During the lysis stage of development, when ER is infectious, we showed the overexpression of a transcription factor, dksA, which is also known to induce virulence in other pathogens such as Salmonella typhimurium. Our results suggest a possible role of these genes in promoting ER development and pathogenicity.
Using Efficient Anchoring Points for Generating Search Directions in Interior Multiobjective Linear Programming (1994)

Ami Arbel, Using Efficient Anchoring Points for Generating Search Directions in Interior Multiobjective Linear Programming, The Journal of the Operational Research Society, Vol. 45, No. 3 (Mar., 1994), pp. 330-344
Sub-1V capacitor-free low-power-consumption LDO with digital controlled loop (2008)

A CMOS sub-1V capacitor-free low-power-consumption low-dropout voltage regulator (LDO) with digital controlled loop is presented in this paper. This technique can make power consumption lower than other LDOs with traditional controlled Loop. Especially, the performance of power consumption of proposed LDO without off-chip capacitors is excellent. The LDO can also be stable even without the output capacitor. With 0.9V power supply voltage, the output voltage is designed as 0.6V. The maximum output current of the LDO is 120 mA at an output of 0.6V. The prototype of the LDO is fabricated with TSMC 0.35-μm CMOS processes. The chip area (including I/O pad) is only 927μm × 969μm.
Hyperspectral imaging with a TWINS birefringent interferometer (2019)

We introduce a high-performance hyperspectral camera based on the Fourier-transform approach, where the two delayed images are generated by the Translating-Wedge-Based Identical Pulses eNcoding System (TWINS) [Opt. Lett. 37, 3027 (2012)], a common-path birefringent interferometer that combines compactness, intrinsic interferometric delay precision, long-term stability and insensitivity to vibrations. In our imaging system, TWINS is employed as a time-scanning interferometer and generates high-contrast interferograms at the single-pixel level. The camera exhibits high throughput and provides hyperspectral images with spectral background level of -30dB and resolution of 3 THz in the visible spectral range. We show high-quality spectral measurements of absolute reflectance, fluorescence and transmission of artistic objects with various lateral sizes.
High-Flux Palladium Membranes Based on Microsystem Technology (2004)

Hydrogen-selective Pd membranes can be used to improve the conversion of dehydrogenations, steam reforming, and the water gas shift reaction. The application of these membranes on a large scale is prohibited by the costs of the existing membranes, requiring membranes with a much higher permeability. In this paper we describe the manufacturing of Pd and Pd/Ag membranes using microsystem technology. These membranes exhibit fluxes that can be a factor of 10 higher than the fluxes obtained using porous supports. Despite the thin Pd and Pd/Ag layers applied, these membranes are pinhole-free.
Supplemental Figures and Table from mTOR and HDAC Inhibitors Converge on the TXNIP/Thioredoxin Pathway to Cause Catastrophic Oxidative Stress and Regression of RAS-Driven Tumors (2023)

&lt;p&gt;Supplementary Figure S1. MPNSTs are sensitive to combined HDAC and mTOR inhibtion; Supplementary Figure S2. Change in animal weight while on combiniation treatment; Supplementary Figure S3. A) 90-8TL cells were treated with Nexturastat A at concentration indicated, with (black) or without (white) 100nM sapanisertib; Supplementary Figure S4. Combined HDAC and mTOR inhibition increases reactive oxygen species in MPNSTs;Supplementary Figure S5. TXNIP is required for combination induced MPNST cell death;Supplementary Table S1. Differentially expressed gene sets in MPNSTs treated with HDAC and mTOR inhibitors&lt;/p&gt;
Thermoresponsive PS-b-PNIPAM-b-PS Micelles: Aggregation Behavior, Segmental Dynamics, and Thermal Response (2010)

We have studied I lie thermal behavior of amphiphilic, symmetric triblock copolymers having short, deuterated polystyrene (PS) end blocks and a large poly(N-isopropylacrylarnicle) (PNIPAM) middle block exhibiting a lower critical solution temperature (LCST) in aqueous solution. A wide range of concentrations (0.1-300 mg/mL) is investigated using it number of analytical methods such as fluorescence correlation spectroscopy (FCS), turbidimetry, dynamic light scattering (DLS), small-angle neutron scattering (SANS), and neutron spin-echo spectroscopy (NSE). The critical micelle concentration is determined using FCS to be 1 mu M or less. The collapse of the micelles at the LCST is investigated using turbidimetry and DLS and shows a weak dependence on the degree of polymerization of the PNIPAM block. SANS with contrast matching allows its to reveal the core-shell Structure of the micelles as well as their correlation as a function of temperature. The segmental dynamics of the PNIPAM shell are studied as a function of temperature and arc found to be faster in the collapsed state than in the swollen state. The mode detected has a linear dispersion in q(2) and is found to be faster in the collapsed state as compared to the swollen state. We attribute this result to the averaging over mobile and immobilized segments.
Fracture Approaches to Debonding in FRP Systems for Double Sided Flexural Upgrade (2012)

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Reorientation and isomerization of trans-stilbene in alkane solutions (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReorientation and isomerization of trans-stilbene in alkane solutionsSeong K. Kim and Graham R. FlemingCite this: J. Phys. Chem. 1988, 92, 8, 2168–2172Publication Date (Print):April 1, 1988Publication History Published online1 May 2002Published inissue 1 April 1988https://pubs.acs.org/doi/10.1021/j100319a018https://doi.org/10.1021/j100319a018research-articleACS PublicationsRequest reuse permissionsArticle Views309Altmetric-Citations115LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Zinc blocks gene expression of mitochondrial aconitase in human prostatic carcinoma cells (2005)

Mitochondrial aconitase (mACON) contains a [4Fe-4S] cluster as the key enzyme for citrate oxidation in the human prostatic epithelial cell. Although there is accumulating evidence indicating that accumulation of high levels of zinc in prostate epithelial cells causes reduced efficiency of citrate oxidation, zinc regulation on the mACON is still not well understood. From in vitro studies, zinc chloride treatment has been developed using humic acid as the carrier (Zn-HA) in human prostatic carcinoma cells, PC-3. Zn-HA treatment (0.1-10 microM) restricts mACON enzymatic activity, which attenuates citrate utility and decreases intracellular ATP levels in PC-3 cells, whereas the effect is blocked by adding the zinc chelator, diethylenetriaminepentaacetic acid (DTPA). Immunoblot, ribonuclease-protection and transient gene-expression assays indicate that Zn-HA treatments inhibit mACON gene expression. Mutation of the putative metal response element (MRE) from CTCGCCTTCA to TGATCCTTCA abolishes Zn-HA inhibition of mACON promoter activity. Our results have demonstrated that zinc possesses a specific regulatory mechanism on the mACON gene, and a biologic function of the putative metal regulatory system in mACON gene transcription has been identified.
Experiments on the influence of low frequency sound on the acoustic resonances in a corrugated flow pipe (2010)

It is well known that an air flow in a corrugated pipe might excite the longitudinal acoustic modes of the pipe. In this letter is reported experiments where a low frequency, oscillating flow with velocity magnitudes of the same order as the air flow has been added. Depending on the oscillation strength, it might silence the pipe or move the resonances to higher harmonics. It is also shown that a low frequency oscillation by itself might excite a higher frequency acoustic resonance of the pipe.
Design of Cooperative System Consisting of Multiple Position Controlled Robots. (2000)

A simple strategy for carrying a large object with the cooperation of multiple position-controlled robots is proposed. In cooperative systems handling single objects, compliance is necessary for each robot to avoid excessive inner forces caused by mutual positioning errors. However, since the majority of commercial industrial robots have PID position controllers, the necessary compliance cannot be achieved by servo systems. Thus, mechanisms consisting of passive joints are installed in a cooperative System as mechanical compliance. First, the mechanical compliance required for the cooperation of position-controlled robots is derived. From the theoretical results, it is determined that the maximum number of robots that can directly support a single object is six. Second, the statics and error characteristics of cooperative systems including compliant mechanisms are analyzed. An example of the cooperation of three mobile robots is designed using passive joint mechanisms. Finally, the strategy is extended to cooperation among an arbitrary number of mobile robots by building hierarchical structures with passive joints.
Vom historischen zum erinnerten Jesus. Der erinnerte Jesus als neues Paradigma der Jesusforschung (2007)

Was gibt es Neues vom historischen Jesus? Seit Beginn der 80er Jahre schien eine neue Epoche angebrochen zu sein, die als „dritte Fragerunde“ (= “third quest“) der historischen Jesusforschung bezeichnet wird. Im Zentrum stand dabei die verstarkte Wahrnehmung der judischen Lebenswelt Jesu. Methodologisch gewannen sozialwissenschaftliche Zugange an Bedeutung, und neben den neutestamentlichen Zeugnissen erfuhren auserkanonische Texte, wie etwa das Thomasevangelium, verstarkte Aufmerksamkeit. Mehr als zwei Jahrzehnte spater lassen sich wiederum neue Forschungstrends beobachten. Besonders charakteristisch treten ein gesteigertes Interesse an der Archaologie Palastinas, eine differenzierte Wurdigung der Logienquelle Q und ein neu entfachtes Interesse an der Erforschung mundlicher Uberliefungsmechanismen der Jesustradition (v.a. Bailey) hervor. Konkret werden einige Monographien (Dunn, Bauckham, Theisen, Hurtado) vorgestellt und kritisch gewurdigt. Insgesamt verdichtet sich dabei der Eindruck einer Bewegung hin zu einer bereits vorosterlich christologischen und damit theologischen Wahrnehmung des erinnerten Jesus.
Robust Parameter Design Using Generalized Linear Mixed Models (2006)

In robust parameter design, it is often the case that the quality characteristic is nonnormal. An example in semiconductor manufacturing is resistivity, which typically follows a gamma distribution. It is also common to have models that contain, in addition to fixed polynomial effects, a random effect representing an extraneous source of variation. In this article, we demonstrate the use of generalized linear mixed models (GLMM) for situations in which the response is nonnormal and in which the noise variable is a random effect. We discuss the analysis of the random effects as well as the fixed effects in a fitted model using GLMM techniques. A numerical example from semiconductor manufacturing is provided for illustration.