gebhart/patrepeval huggingface dataset:
Limited proteolysis of creatine kinase. Implications for three-dimensional structure and for conformational substates (1993)

Proteinase K, subtilisin, pronase E, elastase, bactotrypsin, and thermolysin are all shown here to cleave native mitochondrial creatine kinase from chicken heart (Mib-CK) very specifically at a single site, either before or after Ala-323. In analogy with hen egg ovalbumin, where the same proteases all cleaved the polypeptide chain very specifically around Ala-352, Ala-323 of Mib-CK may be located in an exposed surface loop that is sensitive to protease attack. Gel permeation chromatography demonstrated that the two proteolytic fragments of Mib-CK with M(r)'s of approximately 37,000 and approximately 6000 remain associated with each other. Proteinase K cleavage did not influence the octamer to dimer ratio of Mib-CK, indicating that selective cleavage after Ala-323 has no direct effect on dimer-dimer interfaces within the octamer. However, upon addition of MgADP plus creatine and nitrate to induce a transition-state analogue complex of the enzyme, native Mib-CK dissociated much more readily into dimers than proteinase K-digested Mib-CK. Furthermore, proteinase K cleavage of Mib-CK resulted in 2-11-fold decreases in the Vmax values, as well as in 6-23-fold increases in the Km values for phosphocreatine, creatine, and MgATP, whereas the Kd values for both MgATP and creatine were unaffected. Consequently, proteinase K cleavage of Mib-CK does not affect substrate binding per se, but interferes with substrate-induced conformational changes which are essential for catalysis and which mediate the synergism in substrate binding as it is observed with the unmodified enzyme.(ABSTRACT TRUNCATED AT 250 WORDS)
Transmission Electron Microscopy Confirmation of Orientia tsutsugamushi in Human Bile (2020)

Scrub typhus, the third most frequently reported infectious disease in South Korea, causes serious public health problems. In 2019, we collected a bile specimen from a patient with scrub typhus through percutaneous transhepatic gallbladder drainage and performed transmission electron microscopy to confirm the ultrastructure of Orientia tsutsugamushi.
Amino acid substitutions in VH CDR2 change the idiotype but not the antigen-binding of monoclonal antibodies to alpha(1----6)dextrans. (1989)

An idiotype defined by mAb and polyclonal antibodies to 10.16.1, an anti-alpha(1----6) dextran was previously reported to be expressed on most BALB/c anti-alpha(1----6)dextrans with groove-type sites and to involved CDR3 and probably CDR2. By comparing amino acid sequences of VH and VL derived from cDNA of idiotype+ and idiotype- anti-alpha(1----6)dextran hybridoma proteins, an idiotope was assigned to VH CDR2. Substitution of phenylalanine for leucine at residue 52 in CDR2 coupled with amino acid changes at either residue 58 or residues 57 and 60 abolished expression of this idiotype without affecting Ag binding.
DNA electrophoresis in polymer solutions: Ogston sieving, reptation and constraint release (1993)

The electrophoresis of long polyelectrolytes is considered theoretically, with special attention to duplex DNA. We first discuss quantitative approaches to determine unambiguously the entanglement properties of polymer solutions. Following an idea proposed by Grossman and Soane, we then assume that the "mesh" size of the solution plays the role of a dynamic "pore size" in order to apply theories for gel electrophoresis. In the framework of the Ogston model, we predict that duplex DNA up to 1 kb or more should be separable in dilute (i.e. nonentangled) solutions of high molecular weight polymers. In an entangled solution, and for DNA larger than the pore size, we use a recently developed fluctuation-reptation model to predict the range of sizes in which separation should be possible as a function of electric field E and pore size zeta b. For zeta b larger than the Kuhn length of DNA, we predict a separation up to a size N*scaling as E-1 zeta b-1. For zeta b smaller than the Kuhn length, two different regimes are expected. For small electric fields (typically of the order of 10 V/cm), N*should be proportional to E-1 zeta b-3/2, whereas for high electric fields such as encountered in capillary electrophoresis, we expect that N*is proportional to E-2/5 zeta b-12/5. These predictions are qualitatively different from earlier ones. Finally, we demonstrate that the finite lifetime of the "pores" in an entangled solution (as opposed to a gel) may lead to a new migration mechanism by constraint release, which is not size-dependent.(ABSTRACT TRUNCATED AT 250 WORDS)
Co-Channel Interference Cancellation Using Single Radio Frequency and Baseband Chain (2010)

We present a new architecture for multi-antenna receivers that cancels the co-channel interference (CCI) using a single radio frequency (RF) and baseband (BB) chain, while still achieving nearly the same bit error rate that can be provided by the conventional receiver architecture requiring multiple RF/BB chains. The proposed receiver architecture enables multiple transmitter-receiver pairs to simultaneously communicate in the same frequency band without additional bandwidth, thereby increasing the spectral efficiency or capacity, with significantly reduced receiver complexity and power consumption.
Fabrication and primary photoevents in self-assembled nanocomposites based on semiconductor quantum dots and tetrapyrrole chromophores (2005)

The directed surface passivation of semiconductor CdSe, 0r CdSe/ZnS quantum dots (QD) by meso-pyridyl substituted porphyrins (H<sub>2</sub>P) has been realized via a reversible non-covalent self-assembly interaction of H<sub>2</sub>P meso-pyridyl nitrogens with ions of the ZnS shell or Cd atoms of the CdSe core in various solvents at ambient temperature. The formation of "QD-porphyrin" nanoassemblies leads to a QD photoluminescence (PL) quenching (intensity decrease and PL decay shortening) accompanied by a H<sub>2</sub>P fluorescence enhancement. The analysis of experimental Foerster resonance energy transfer efficiencies EFRET (FRET) found via acceptor (H<sub>2</sub>P) sensibilization and donor (QD) PL quenching shows that EFRET values obtained from fluorescence enhancement are of the order of 6 - 8 % for most QD studied and are thus much smaller as compared to the PL quenching efficiency. With respect to QD PL quenching efficiencies, smaller values of EFRET might be due to different competing reasons: the presence of two independent quenching processes in the nanoassemblies, energy transfer QD -> H<sub>2</sub>P and photoinduced (electron/hole) charge transfer (CT) or time-dependent QD interface dynamics leading to a noticeable QD PL quenching. The analysis of spectroscopic and kinetic findings reveals that a limited number of "vacancies" accessible for porphyrin attachment is available on the QD surface. Simultaneous presence of porphyrin triads/pentads and QDs in a solution leads to the formation of higly organzed nanoassemblies.
Parameters Controlling High Temperature Deformability of Wrought Magnesium Alloys (2014)

Due to limited deformability at room temperature, high temperature forming of magnesium alloys appears as an interesting alternative. Superplastic properties can be obtained in the case of fine grained magnesium alloys and in this regime, due to significant damage sensitivity, fracture strain is mainly controlled by nucleation, growth and coalescence of cavities. Magnesium alloys with large grained alloys can also exhibit interesting deformabilities at high temperature since dislocation movements can be controlled by a solute drag effect promoting plastic stability. Examples of such situations are presented in the case of wrought magnesium alloys, the associated damage mechanisms being investigated thanks to 3D X-ray micro tomography performed in continuous mode, namely directly during high temperature deformation tests.
Pharmacological characterization of 5‐HT4 receptors mediating relaxation of canine isolated rectum circular smooth muscle (1999)

This study aimed to characterize for the first time in vitro 5-HT4 receptors in the canine gastrointestinal tract. For this purpose, we used circular muscle strips of the canine isolated rectum. In the presence of methysergide (60 microM), 5-HT induced relaxation of methacholine (1 microM)-precontracted muscle strips, yielding a monophasic sigmoidal concentration-relaxation curve (pEC50 7.2+/-0.07). Tetrodotoxin (0.3 microM) did not affect the curve to 5-HT, suggesting the inhibitory 5-HT receptor is located on the smooth muscle. Granisetron (0.3 microM) did also not affect the curve to 5-HT, which excludes the 5-HT3 receptor mediating the relaxation to 5-HT. The presence of methysergide rules out the involvement of 5-HT1, 5-HT2 or 5-HT7 receptors. 5-HT, the selective 5-HT4 receptor agonists R076186, prucalopride (R093877) and SDZ HTF-919 and the 5-HT4 receptor agonists cisapride and 5-MeOT relaxed the muscle strips with a rank order of potency R076186 = 5-HT > cisapride > prucalopride > or = SDZ HTF-919 > 5-MeOT. The selective 5-HT4 receptor antagonists GR 125487, RS 39604 and GR 113808 competitively antagonized the relaxations to 5-HT, yielding pK(B) estimates of 9.7, 7.9 and 9.1, respectively. The selective 5-HT4 receptor antagonist SB 204070 shifted the curve to 5-HT rightward and depressed the maximal response (apparent pA2 10.6). GR 113808 (10 nM) produced a parallel rightward shift of the curve to the selective 5-HT4 receptor agonists R076186 (pA2 8.8). It is concluded that 5-HT induces relaxation of the canine rectum circular muscle through stimulation of a single population of smooth muscle 5-HT4 receptors. For the first time, a nonhuman species was shown to exhibit relaxant 5-HT4 receptors in the large intestine.
Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts (2016)

High hydrostatic pressure (HHP) has been used to pre-condition embryos before essential, yet potentially detrimental procedures such as cryopreservation. However, the mechanisms for HHP are poorly understood. We treated bovine blastocysts with three different HHP (40, 60 and 80 MPa) in combination with three recovery periods (0, 1 h, 2 h post HHP). Re-expansion rates were significantly higher at 40 and 60 but lower at 80 MPa after vitrification-warming in the treated groups than controls. Microarray analysis revealed 399 differentially expressed transcripts, representing 254 unique genes, among different groups. Gene ontology analysis indicated that HHP at 40 and 60 MPa promoted embryo competence through down-regulation of genes in cell death and apoptosis, and up-regulation of genes in RNA processing, cellular growth and proliferation. In contrast, 80 MPa up-regulated genes in apoptosis, and down-regulated protein folding and cell cycle-related genes. Moreover, gene expression was also influenced by the length of the recovery time after HHP. The significantly over-represented categories were apoptosis and cell death in the 1 h group, and protein folding, response to unfolded protein and cell cycle in the 2 h group compared to 0 h. Taken together, HHP promotes competence of vitrified bovine blastocysts through modest transcriptional changes.
Nanoparticles as Smart Treatment-delivery Systems in Plants: Assessment of Different Techniques of Microscopy for their Visualization in Plant Tissues (2007)

The results open a wide range of possibilities for using magnetic nanoparticles in general plant research and agronomy. The nanoparticles can be charged with different substances, introduced within the plants and, if necessary, concentrated into localized areas by using magnets. Also simple or more complex microscopical techniques can be used in localization studies.
NCPV photovoltaics program review : proceedings of the 15th conference, Denver, Colorado September 1998 (1999)

This proceedings volume compiles 123 papers that were presented orally or as posters at the National Center for Photovoltaics (NCPV) Program Review Meeting, held in Denver, Colorado, on September 8-11, 1998. The purpose of this meeting was to highlight the advances made in various areas of photovoltaics by and through the NCPV during the period of December 1997 to September 1998. Topics covered ranged from research in crystalline silicon and thin-film technologies, to manufacturing of photovoltaic modules, to applications of and markets for photovoltaic products.
Network based Steganography (2013)

Steganography, introduced in 2003, is a techniques used for hidden communication between two covert parties. It is an art of hidden communication. It also relates to the areas like network protocols and security for practical data hiding in communication networks using Transmission Control Protocol/Internet Protocol (TCP/IP). The typical steganographic method utilizes digitized media files (images, video and audio files) as a cover medium for hiding data, network steganography uses communication protocols such as TCP/IP. Such methods make it harder to detect and eliminate. In a typical steganography using network the modification of a single network protocol occurs. Such modification can be applied to the Protocol Data Unit. Network steganography sheltersa broad spectrum of techniques.
Synthesis of substituted indazole-5,6-dicarbonitriles (2012)

Methods have been developed for the synthesis of previously unknown N-substituted 1H-indazole-5,6-dicarbonitriles from 4-methyl-5-nitrophthalonitrile.
Determination of superoxide anion radicals generated by human polymorphonuclear leukocytes using spin trapping technique. (1989)

Electron spin resonance (ESR) is regarded as the least ambiguous method for the detection of free radicals. Using spin trapping technique with 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO), we measured superoxide anion radicals generated by human polymorphonuclear leukocytes (PMN) stimulated with various agents. PMN were isolated from heparinized venous blood of healthy volunteers by dextran sedimentation followed by Ficoll-Paque separation and hypotonic lysis of contaminating erythrocytes, and suspended in Hanks' balanced salt solution at pH 7.4. Phorbol myristate acetate (PMA), opsonized zymosan (OZ), digitonin, and Ca ionophore (A23187) were used as stimulants of PMN. ESR spectra were obtained by JEOL-JES-FR80 ESR spectrometer, and the intensity of the signal of DMPO-OOH and DMPO-OH adduct was measured as the ratio to the intensity of Mn2+ signal.The ESR spectra produced by stimulated PMN in the presence of DMPO was composed of DMPO-OOH adduct and DMPO-OH adduct, however, the composite ratio of signal intensity of DMPO-OH adduct to that of DMPO-OOH addutt differed with stimulants of PMN. The relative intensity of DMPO-OOH signal increased in proportion to the cell concentration, indicating that semi-quantitative determination of superoxide generated by stimulated PMN is possible.
Intelligent Sliding Mode Control of an Overhead Container Crane (2022)

In this contribution, an intelligent controller is proposed for an underactuated overhead container crane subject to both parameter uncertainties and unmodeled dynamics. The adopted approach is based on the sliding mode method to confer robustness against modeling inaccuracies and external disturbances. Additionally, an adaptive fuzzy inference system is embedded within the control law to improve set-point regulation and trajectory tracking. In order to evaluate the performance of the proposed intelligent scheme, the control law was implemented and tested in a 1:6 scale experimental container crane, available at the Institute of Mechanics and Ocean Engineering at Hamburg University of Technology. The obtained experimental results demonstrate not only the feasibility of the proposed scheme, but also its improved efficacy for both stabilization and trajectory tracking problems.
Prostaglandin F2α Facilitates Platelet Activation by Acting on Prostaglandin E2 Receptor Subtype EP3 and Thromboxane A2 Receptor TP in Mice (2019)

Platelets play an important role in both physiological hemostasis and pathological thrombosis. Thromboxane (TX) A<sub>2</sub> and prostaglandin (PG) I<sub>2</sub> are well known as a potent stimulator and an inhibitor of platelet function, respectively. Recently, PGE<sub>2</sub> has also been reported to regulate platelet function via PGE<sub>2</sub> receptor subtypes. However, the effect of PGF<sub>2</sub>α on platelet function remains to be determined. The aim of the present study was to clarify the effect of PGF<sub>2</sub>α on murine platelet function both in vitro and in vivo. Platelets prepared from wild-type mice (WT platelets) expressed several types of prostanoid receptors, including the PGE<sub>2</sub> receptor subtype EP<sub>3</sub> and the TXA<sub>2</sub> receptor TP, while expression of the PGF<sub>2</sub>α receptor FP was not detected. In WT platelets, PGF<sub>2</sub>α potentiated adenosine diphosphate-induced aggregation in a concentration-dependent manner, while PGF<sub>2</sub>α alone did not induce aggregation. In platelets prepared from mice lacking FP, however, PGF<sub>2</sub>α-induced potentiation was not significantly different from that in WT platelets. Interestingly, the potentiation was significantly blunted in platelets lacking EP<sub>3</sub> or TP and disappeared completely in platelets lacking both EP<sub>3</sub> and TP. Accordingly, PGF<sub>2</sub>α decreased the cyclic adenosine monophosphate level via EP<sub>3</sub> and increased the inositol triphosphate level via TP in WT platelets. Intravenously administered PGF<sub>2</sub>α significantly shortened the bleeding time and aggravated arachidonic acid-induced acute thromboembolism in WT mice, suggesting that PGF<sub>2</sub>α works as a platelet stimulator also in vivo. In conclusion, PGF<sub>2</sub>α potentiates platelet aggregation in vitro via EP<sub>3</sub> and TP but not FP. Accordingly, PGF<sub>2</sub>α facilitates hemostasis and thromboembolism in vivo.
CHAPTER XXIV (2011)

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ChemInform Abstract: Pd‐Catalyzed α‐Arylation of Nitriles and Esters and γ‐Arylation of Unsaturated Nitriles with TMPZnCl·LiCl. (2011)

Abstract The reactions of α,β‐ and β,γ‐unsaturated nitriles proceeds regioselectively.
An Auction Approach to Resource Allocation in Uplink OFDMA Systems (2009)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> We propose resource allocation algorithms based on the auction method for uplink OFDMA cellular networks. We consider cellular systems that employ the traditional static frequency reuse as well as the next-generation systems that aim to achieve a universal frequency reuse via base-station coordination. Our algorithms are designed for finite input alphabets and also account for nonideal practical outer codes, and they can be implemented in a distributed manner, when applied for multicell resource allocations. We also address power and bandwidth constraints that are motivated by practical concerns. The proposed algorithms exhibit very low complexity and are well suited for parallel implementations. Simulation results demonstrate that they offer near-optimal performance. </para>
RESULTS OF AN ACTIVE ROBOTIC TOTAL KNEE ARTHROPLASTY US TRIAL (2020)

Introduction Active robotics for total knee Arthroplasty (TKA) uses a CAD-CAM approach to plan the correct size and placement of implants and to surgically achieve planned limb alignment. The TSolu...
Characterization of heterojunction laser diodes by near field optical scanning microscopy: layer composition and mode structure analysis (2002)

We report mode structure and semiconductor layer composition analysis of strained (In,Ga)As quantum well lasers using the super-resolution capabilities of near field scanning optical microscopy (NSOM). Submicron collection mode imaging of the emission mode structure easily identifies high order transverse modes as well as mode leakage into the substrate. Using the tip as a localized, tunable source of photons, near field optical beam induced current (NOBIC) measurements reveals the compositional profile of the layer structure. The active region is very sensitive to the excitation wavelength due to the effect of a changing optical absorption in near field coupling to a thin layer.
The Solubility of Mercury in Hydrocarbons (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Solubility of Mercury in HydrocarbonsRobert R. Kuntz and Gilbert J. MainsCite this: J. Phys. Chem. 1964, 68, 2, 408–410Publication Date (Print):February 1, 1964Publication History Published online1 May 2002Published inissue 1 February 1964https://doi.org/10.1021/j100784a501RIGHTS & PERMISSIONSArticle Views237Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (342 KB) Get e-Alerts
Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity (2008)

The main antiatherogenic function of HDL is to promote the efflux of cholesterol from peripheral cells and transport it to the liver for excretion in a process termed reverse cholesterol transport. The aim of this study was to evaluate the cholesterol efflux capacity in low- and high-HDL subjects by utilizing monocytes and serum from 18 low-HDL and 15 high-HDL subjects. Low and high HDL levels were defined, respectively, as HDL < or =10(th) and HDL > or =90(th) Finnish age/sex-specific percentile. Cholesterol efflux from [(3)H]cholesterol-oleate-acetyl-LDL-loaded monocyte-derived macrophages to standard apolipoprotein A-I (apoA-I), HDL(2), and serum was measured. In addition, cholesterol efflux from acetyl-LDL-loaded human THP-1 macrophages to individual sera (0.5%) derived from the study subjects was evaluated. Cholesterol efflux to apoA-I, HDL(2), and serum from macrophage foam cells derived from low- and high-HDL subjects was similar. The relative ABCA1 and ABCG1 mRNA expression levels in unloaded macrophages, as well as their protein levels in loaded macrophage foam cells, were similar in the two study groups. Cholesterol efflux from THP-1 foam cells to serum recovered from high-HDL subjects was slightly higher than that to serum from low-HDL subjects (P = 0.046). Cholesterol efflux from THP-1 macrophages to serum from study subjects correlated with serum apoB (P = 0.033), apoA-I (P = 0.004), apoA-II (P < 0.0001), and the percentage of apoA-I present in the form of prebeta-HDL (P = 0.0001). Our data reveal that macrophages isolated from either low- or high-HDL subjects display similar cholesterol efflux capacity to exogenous acceptors. However, sera from low-HDL subjects have poorer cholesterol acceptor ability as compared with sera from high-HDL subjects.