gebhart/patrepeval huggingface dataset:
Recombinant human lecithin‐cholesterol acyltransferase Fc fusion: Analysis of N‐ and O‐linked glycans and identification and elimination of a xylose‐based O‐linked tetrasaccharide core in the linker region (2013)

Recombinant human lecithin-cholesterol acyltransferase Fc fusion (huLCAT-Fc) is a chimeric protein produced by fusing human Fc to the C-terminus of the human enzyme via a linker sequence. The huLCAT-Fc homodimer contains five N-linked glycosylation sites per monomer. The heterogeneity and site-specific distribution of the various glycans were examined using enzymatic digestion and LC-MS/MS, followed by automatic processing. Almost all of the N-linked glycans in human LCAT are fucosylated and sialylated. The predominant LCAT N-linked glycoforms are biantennary glycans, followed by triantennary sugars, whereas the level of tetraantennary glycans is much lower. Glycans at the Fc N-linked site exclusively contain typical asialobiantennary structures. HuLCAT-Fc was also confirmed to have mucin-type glycans attached at T407 and S409 . When LCAT-Fc fusions were constructed using a G-S-G-G-G-G linker, an unexpected +632 Da xylose-based glycosaminoglycan (GAG) tetrasaccharide core of Xyl-Gal-Gal-GlcA was attached to S418 . Several minor intermediate species including Xyl, Xyl-Gal, Xyl-Gal-Gal, and a phosphorylated GAG core were also present. The mucin-type O-linked glycans can be effectively released by sialidase and O-glycanase; however, the GAG could only be removed and localized using chemical alkaline β-elimination and targeted LC-MS/MS. E416 (the C-terminus of LCAT) combined with the linker sequence is likely serving as a substrate for peptide O-xylosyltransferase. HuLCAT-Fc shares some homology with the proposed consensus site near the linker sequence, in particular, the residues underlined PPPE416 GS418 GGGGDK. GAG incorporation can be eliminated through engineering by shifting the linker Ser residue downstream in the linker sequence.
The SRN1 mechanism in heteroaromatic nucleophilic substitution. Reactions involving certain dihalogenated .pi.-deficient nitrogen heterocycles (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe SRN1 mechanism in heteroaromatic nucleophilic substitution. Reactions involving certain dihalogenated .pi.-deficient nitrogen heterocyclesDavid R. Carver, Thomas D. Greenwood, James S. Hubbard, Andrew P. Komin, Yesh P. Sachdeva, and James F. WolfeCite this: J. Org. Chem. 1983, 48, 8, 1180–1185Publication Date (Print):April 1, 1983Publication History Published online1 May 2002Published inissue 1 April 1983https://pubs.acs.org/doi/10.1021/jo00156a007https://doi.org/10.1021/jo00156a007research-articleACS PublicationsRequest reuse permissionsArticle Views322Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Uncertainty evaluation for the measurement of gauge blocks by optical interferometry (1997)

NRC publication: Yes
Indoor Tracking of Dementia Patients without GPS (2022)

Dementia is one of the most common conditions that can be seen in elderly people. Often patients suffering from dementia lose their way home or wander in the streets. The caretakers find it difficult to keep track of the patient&#x0027;s movements. The Real-time locating systems (RTLS) is a technology that is used to track and locate objects or people. RTLS can be used in the hospital to track the movements of the patients. This research work proposes an RTLS based method to track the movements of the patients suffering from dementia to stop them from getting out of the house or care centers. So that they would not get lost in the streets. The proposed system uses a NodeMCU based wearable device that helps in tracking patient. The necessary counter actions are deployed if the patient tries to leave the care center. This proposed system enables the caretaker to monitor and track the movements of the patient. The unique aspect of the proposed system is that it does not use GPS (global pointing system) and the NodeMCUs can be networked locally so that external factors like internet outrage will not affect the system. The Internet can be used if needed.
Structure and expression of STK, a src-related gene in the simple metazoan Hydra attenuata. (1989)

Both cDNA clones and a genomic DNA clone encoding a 509-amino-acid protein that is 64% similar to chicken pp60c-src were isolated from the simple metazoan Hydra attenuata. We have designated this gene STK, for src-type kinase. Features of the amino acid sequence of the protein encoded by the STK gene suggest that it is likely to be myristoylated and regulated by phosphorylation in a manner similar to that found for pp60c-src. The genomic sequence encoding the protein was found to be interrupted by at least two introns, one of which was located in a position identical to that of one of the introns in the chicken src gene. The STK gene was expressed during early development of H. attenuata and at high levels in the epithelial cells of adult polyps. Probing of Hydra proteins with an antibody to phosphotyrosine indicated that the major phosphotyrosine-containing protein in H. attenuata may be the STK protein itself. H. attenuata is the simplest organism from which a protein-tyrosine kinase gene has been isolated. The presence of such a gene in the evolutionarily ancient phylum Cnidaria suggests that protein-tyrosine kinase genes arose concomitantly with or shortly after the appearance of multicellular organisms.
Salicylic acid: an old hormone up to new tricks (2013)

Salicylic acid (SA) acts as a signalling molecule in plant defence against biotrophic and hemibiotrophic phytopathogens. The biosynthesis of SA on pathogen detection is essential for local and systemic acquired resistance, as well as the accumulation of pathogenesis-related (PR) proteins. SA biosynthesis can occur via several different substrates, but is predominantly accomplished by isochorismate synthase (ICS1) following pathogen recognition. The roles of BTB domain-containing proteins, NPR1, NPR3 and NPR4, in SA binding and signal transduction have been re-examined recently and are elaborated upon in this review. The pathogen-mediated manipulation of SA-dependent defences, as well as the crosstalk between the SA signalling pathway, other plant hormones and defence signals, is also discussed in consideration of recent research. Furthermore, the recent links established between SA, pathogen-triggered endoplasmic reticulum stress and the unfolded protein response are highlighted.
Determination of spatially differentiated water balance components including groundwater recharge on the Federal State level – A case study using the mGROWA model in North Rhine-Westphalia (Germany) (2015)

Study region: The Federal State of North Rhine-Westphalia (NRW), Germany. Study focus: On behalf of the Federal State Agency for Nature, Environment and Consumer Protection of North Rhine-Westphalia, Germany (LANUV) the mGROWA model is applied, in order to simulate the water balance components actual evapotranspiration, total runoff, direct runoff and groundwater recharge at Federal State level. mGROWA-simulations were performed in daily time steps for the hydrological reference period 1971–2000 and in a spatial resolution of 100 by 100 m. mGROWA results for groundwater recharge and State-wide recorded groundwater withdrawal rates have been used to assess the extent of groundwater exploitation in NRW. New hydrological insights: Simulated groundwater recharge levels are presented as long-term annual averages and as long-term monthly values in order to indicate the seasonal fluctuation of groundwater recharge rates. Quantitative statistics indicate that mGROWA enables the simulation of total runoff and groundwater recharge without significant tendency of over- or underestimation. Against this background mGROWA simulation results are used by LANUV to support regional water resources management, e.g. for determining the status of groundwater exploitation in NRW. The majority of groundwater bodies in NRW are at present not at risk of unsustainable groundwater usage. A small number of groundwater bodies along the river Rhine, however, have been assessed as being close to unsustainable groundwater exploitation.
Dynamics of the protein structure of T169S pyranose 2-oxidase in solution: Molecular dynamics simulation (2017)

Pyranose 2-oxidase (P2O) from Trametes multicolor contains FAD as cofactor, and forms a tetramer. The protein structure of a mutated P2O, T169S (Thr169 is replaced by Ser), in solution was studied by means of molecular dynamics simulation and analyses of photoinduced electron transfer (ET) from Trp168 to excited isoalloxazine (Iso*), and was compared with wild type (WT) P2O. Hydrogen bonding between Iso and nearby amino acids was very similar as between T169S and WT protein. Distances between Iso and Tyr456 were extremely heterogeneous among the subunits, 1.7 (1.5 in WT) in subunit A (Sub A), 0.97 (2.2 in WT) in Sub B, 1.3 (2.1 in WT) in Sub C, 1.3 nm (2.0 in WT) in Sub D. Mean values of root of mean square fluctuation over all residues were greater by four times than those in WT. This suggests that the protein structure of T169S is much more flexible than that of WT. Electrostatic (ES) energies between Iso anion in one subunit and ionic groups in the entire protein were evaluated. It was found that more than 50% of the total ES energy in each subunit is contributed from other subunits. Reported fluorescence decays were analyzed by a method as WT, previously reported. Electron affinities of Iso* in T169S were appreciably higher than those in WT. Static dielectric constants near Iso and Trp168 were also quite higher in T169S than those in WT.
Design and construction of a bus bar for spike reduction in an industrial inverter (2010)

This paper presents a practical method to improve inverter dc bus bar configuration in order to minimize voltage spike. Many parameters are affected by voltage spike such as dc capacitor bank size, EMI noise reduction, elimination of snubber and finally voltage stability and IGBT safety. This approach proposes planar bus bars with minimum space and maximum overlapping between them in order to reduce bus bars stray inductance. Simulation of bus bar structures gives basic idea about which structure provides less stray inductance. To confirm advantages of these method experimental results from an 80-kVA UPS are presented which shows 63% voltage spike reduction.
Adaptive image watermarking using human perception based fuzzy inference system (2018)

Development of digital content has increased the necessity of copyright protection using watermarking. Imperceptibility and robustness are two important features of watermarking algorithms. The goal of watermarking methods is to satisfy the tradeoff between these two contradicting characteristics. Recently, watermarking methods in transform domains have displayed favorable results. In this paper, we present an adaptive blind watermarking method, which has high imperceptibility in areas that are important to the human visual system. We propose a fuzzy system to control the embedding strength factor adaptively. Image saliency, intensity, and edge-concentration are shown to be important to a human observer and are hence used as fuzzy attributes. Embedding is performed in the discrete cosine transform of the wavelet domain to achieve high imperceptibility and acceptable robustness. Experimental results show the superiority of the proposed algorithm over comparable methods.
FOREIGN BIRTH AND FOREIGN TRAVEL AS RISK FACTORS FOR CHILDHOOD LEAD POISONING: FINDINGS FROM A CASE-CONTROL STUDY OF NEW YORK CITY CHILDREN (2004)

ISEE-359 Introduction: Children who were born or have traveled outside of the U.S. are over-represented among lead poisoned children in New York City (NYC). This study examined the association between lead poisoning and foreign birth and travel among NYC children. Methods: A matched case-control design involving 203 cases and 203 controls was employed. NYC public health surveillance data were used to identify cases and controls. Cases had a blood lead level (BLL) greater than or equal to 20 mcg/dL or two tests with BLLs of 15-19 mcg/dL, taken at least 3 months apart. Controls had a BLL less than or equal to 5 mcg/dL. All case and control children were less than 18 years of age (mean age=3±2.5 years), and had received their blood test between May and October 2001. Children were individually matched by age, date of test and residential neighborhood. Parents/guardians provided demographic and behavioral information for their children during telephone interviews. Data on building characteristics were obtained from databases maintained by the City of New York. Results: After controlling for generally recognized risk factors for lead poisoning (e.g., building age, peeling or chipping paint, pica), cases were significantly more likely than controls to have been born or have traveled (within 3 months prior to their blood test) outside of the U.S. There were no significant differences between cases and controls in foreign travel after accounting for foreign birth and other risk factors. However, compared to US-born children, the odds of lead poisoning were significantly greater for foreign-born children in the final multivariate model. Discussion: These findings indicate that foreign-born children are at increased risk for lead poisoning. In addition, traditional risk factors for lead poisoning such as building, behavioral and sociodemographic characteristics do not fully explain the relationship between foreign birth and lead poisoning. Further research is needed to understand the sources of exposure to lead among foreign-born children.
Erythropoietin: A Paradigm for the Development of Practice Guidelines (2001)

Erythropoietin (EPO) is an endogenous hormone produced in the kidney that regulates red blood cell production within the body. Since the cloning and first clinical introduction of recombinant erythropoietin (epoetin) in the late 1980s indications and usage of epoetin have expanded significantly. It is estimated that as many as one third of patients with substantial anemia (hemoglobin less than 10.0 g/dL) resulting from chemotherapy for cancer are treated with epoetin. Though use of epoetin may avoid the inconvenience and infectious risk of blood transfusions, it is expensive and its benefit in some clinical scenarios has been modest. Like many new technologies, strong evidence suggesting situations where the benefit is high has lagged behind its adoption by patients and practitioners. As well, epoetin is expensive and third party payers do not always reimburse it. Research suggests there is considerable variation in epoetin usage in practice. To provide guidance to hematology/oncology specialists regarding use of epoetin, the American Society of Hematology (ASH) and the American Society of Clinical Oncology (ASCO) proposed that the Agency for Healthcare Research and Quality (AHRQ) fund an evidence review by one of the Evidence-based Practice Centers (EPC) that would be used to develop evidence-based guidelines for members of the society. This review highlights principles of evidence-based medicine, distills and appraises the evidence in the published literature that supports the use of epoetin, and presents evidence-based recommendations for use of epoetin in situations where benefit is substantiated by high-quality studies. As well, this review addresses some of the difficulties of performing clinical research in this area, provocative research findings that will require further study, and suggestions regarding epoetin in those areas where further strong evidence has yet to be developed.
Molecular effects of soy phytoalexin glyceollins in human prostate cancer cells LNCaP (2009)

Glyceollins are soy-derived phytoalexins that have been proposed to be candidate cancer preventive compounds. The effect of the glyceollins on prostate cancer is unknown. The present study examined the molecular effects of soy phytoalexin, glyceollins, on human prostate cancer cell LNCaP to further elucidate its potential effects on prostate cancer prevention. We found that the glyceollins inhibited LNCaP cell growth similar to that of the soy isoflavone genistein. The growth inhibitory effects of the glyceollins appeared to be due to an inhibition of G1/S progression and correlated with an up-regulation of cyclin-dependent kinase inhibitor 1 A and B mRNA and protein levels. By contrast, genistein only up-regulates cyclin-dependent kinase inhibitor 1A. In addition, glyceollin treatments led to down-regulated mRNA levels for androgen responsive genes. In contrast to genistein, this effect of glyceollins on androgen responsive genes appeared to be mediated through modulation of an estrogen- but not androgen-mediated pathway. Hence, the glyceollins exerted multiple effects on LNCaP cells that may be considered cancer preventive and the mechanisms of action appeared to be different from other soy-derived phytochemicals.
Potential effectiveness of minidose aspirin combined with dilazep in anti-platelet therapy. (1988)

The anti-platelet aggregating action of minidose aspirin and its combined effect with dilazep were examined in healthy male volunteers. A quantitative and dose-dependent decrease in platelet aggregability was observed on a single oral administration of 30 mg, 100 mg and 300 mg of aspirin and also on the repeated oral administration of 10 mg and 30 mg three times a day for 5 days. Administration of 100 mg of dilazep in combination with 30 mg or 100 mg of aspirin produced an anti-platelet effect equivalent to a 3-fold dose of aspirin alone. In the repeated administration test, the t. i. d. admin-istration of aspirin 10 mg+ dilazep 100 mg was confirmed to be close to the ideal in the balance between anti-platelet action and the reduced production of PGI2
SELECTIVE REDUCTION OF ELEVATED FSH LEVELS IN INFERTILE MEN BY PULSATILE LHRH TREATMENT (1986)

In order to investigate whether isolated elevated FSH levels in men with idiopathic oligospermia can be lowered by pulsatile LHRH therapy, six patients were treated for 6 weeks with 5 micrograms LHRH pulses every 2 h. The pulses were delivered from a portable minipump (Zyklomat) through a subcutaneously inserted needle. At the end of treatment prepulse serum LH levels were no different from the levels before treatment while serum FSH was significantly reduced in all patients (16.9 +/- 2.5 U/l vs 11.3 +/- 1.9 U/l, mean +/- SEM; P less than 0.01). The normal FSH range was reached in one of the six patients. The areas under the LH curves following the first and the last (i.e. 504th) pulse were no different, while the areas under the FSH curves were significantly smaller (2870 +/- 434 vs 1776 +/- 237 U/l X min; P less than 0.01). Serum testosterone and oestradiol were significantly higher at the end of treatment (11.0 +/- 1.2 vs 15.2 +/- 1.9 nmol/l 146 +/- 18 vs 214 +/- 25 pmol, respectively). Thus increased FSH levels in men with idiopathic oligospermia can be selectively reduced by pulsatile LHRH treatment. If the increased FSH levels are not the result but rather a factor contributing to the pathogenesis of certain types of oligospermia these findings may have implications for the treatment of this condition.
Redundant parallel operation of current regulated inverters (2003)

A novel control for redundant parallel inverters with instantaneous current sharing is proposed. Current sharing based upon current regulation is realized through the share bus connecting all the paralleled modules without extra control modules. The share bus consists of only three automatically averaged signals (no matter how many modules in parallel): voltage reference, current reference and feedback voltage. The parallel system operating without extra current deviation control loop is modeled into an equivalent transfer function for the original inverter. As a result, both fast dynamic response and equal load current sharing capability are attained under linear or nonlinear load. System configuration and operation principle are presented. Simulation and experiment results are given in detail.
Software-defined decision-feedback multiuser detection in frequency domain for single-carrier and multi-carrier CDMA systems-a sequential quadratic programming approach (2004)

This paper shows how a unified model can he used to describe the signal received in frequency domain over a multiple-antenna, frequency-selective, multipath fading channel for multicarrier CDMA and single carrier CDMA systems with cyclic prefix. This realization validates the abstraction of these CDMA problems into a mathematical model which can be used for practical problems such as channel estimation and data detection. Moreover, the optimal multiuser detection problem based on the maximal-likelihood criteria for this unified model is addressed as a combinatorial optimization problem. A sequential quadratic programming approach is proposed to approximate this problem, which corresponds to a near-optimal software-defined decision-feedback multiuser receiver for both single-carrier and multicarrier CDMA systems. An efficient gradient projection algorithm is applied for data detection in the proposed receiver. The simulation results show that this scheme can reach near single-user performance even for fully-loaded systems and outperform conventional soft-interference cancellation methods.
THE PENICILLIN RECEPTOR IN STREPTOMYCES* (1974)

peer reviewed
Catalase-driven protein microtube motors with different exterior surfaces as ultrasmall biotools (2021)

We report the synthesis of catalase-driven protein microtube motors with different exterior surfaces. Their abilities of bacteria capture, reaction enhancement by self-stirring, and velocity control with light irradiation were highlighted.
Effect of Small Variation in the Composition of Plates and Weld Filler Wires on the General Corrosion Rate of Ni-Cr-Mo Alloys (2005)

The ASTM standard B 575 provides the requirements for the chemical composition of Nickel-Chromium-Molybdenum (Ni-Cr-Mo) alloys such as Alloy 22 (N06022) and Alloy 686 (N06686). The compositions of each element are given in a range. For example, the content of Mo is specified from 12.5 to 14.5 weight percent for Alloy 22 and from 15.0 to 17.0 weight percent for Alloy 686. It was important to determine how the corrosion rate of welded plates of Alloy 22 using Alloy 686 weld filler metal would change if heats of these alloys were prepared using several variations in the composition of the elements even though still in the range specified in B 575. All the material used in this report were especially prepared at Allegheny Ludlum Co. Seven heats of plate were welded with seven heats of wire. Immersion corrosion tests were conducted in a boiling solution of sulfuric acid plus ferric sulfate (ASTM G 28 A) using both as-welded (ASW) coupons and solution heat-treated (SHT) coupons. Results show that the corrosion rate was not affected by the chemistry of the materials within the range of the standards.