gebhart/patrepeval huggingface dataset:
Hypoxia-induced Regulation of MAPK Phosphatase-1 as Identified by Subtractive Suppression Hybridization and cDNA Microarray Analysis (2001)

Subtractive suppression hybridization was used to generate a cDNA library enriched in cDNA sequences corresponding to mRNA species that are specifically up-regulated by hypoxia (6 h, 1% O(2)) in the oxygen-responsive pheochromocytoma cell line. The dual specificity protein-tyrosine phosphatase MAPK phosphatase-1 (MKP-1) was highly represented in this library. Clones were arrayed on glass slides to create a hypoxia-specific cDNA microarray chip. Microarray, northern blot, and western blot analyses confirmed that MKP-1 mRNA and protein levels were up-regulated by hypoxia by approximately 8-fold. The magnitude of the effect of hypoxia on MKP-1 was approximately equal to that induced by KCl depolarization and much larger than the effects of either epidermal growth factor or nerve growth factor on MKP-1 mRNA levels. In contrast to the calcium-dependent induction of MKP-1 by KCl depolarization, the effect of hypoxia on MKP-1 persisted under calcium-free conditions. Cobalt and deferoxamine also increased MKP-1 mRNA levels, suggesting that hypoxia-inducible factor proteins may play a role in the regulation of MKP-1 by hypoxia. Pretreatment of cells with SB203580, which inhibits p38 kinase activity, significantly reduced the hypoxia-induced increase in MKP-1 RNA levels. Thus, hypoxia robustly increases MKP-1 levels, at least in part through a p38 kinase-mediated mechanism.
Method and tool for network vulnerability analysis (2023)

A computer system analysis tool and method that will allow for qualitative and quantitative assessment of security attributes and vulnerabilities in systems including computer networks. The invention is based on generation of attack graphs wherein each node represents a possible attack state and each edge represents a change in state caused by a single action taken by an attacker or unwitting assistant. Edges are weighted using metrics such as attacker effort, likelihood of attack success, or time to succeed. Generation of an attack graph is accomplished by matching information about attack requirements (specified in "attack templates") to information about computer system configuration (contained in a configuration file that can be updated to reflect system changes occurring during the course of an attack) and assumed attacker capabilities (reflected in "attacker profiles"). High risk attack paths, which correspond to those considered suited to application of attack countermeasures given limited resources for applying countermeasures, are identified by finding "epsilon optimal paths."
NormaChain: A Blockchain-Based Normalized Autonomous Transaction Settlement System for IoT-Based E-Commerce (2018)

Internet of Things (IoT)-based E-commerce is a new business model that relies on autonomous transaction management on IoT-devices. The management system toward IoT-based E-commerce demands autonomy, lightweight, and legitimacy. As blockchain is an innovative technology that is competent in governing the decentralized network, we adopt it to design the autonomous transaction management system on IoT E-commerce. However, current blockchain solutions, most namely cryptocurrencies, have fatal drawbacks of nonsupervisability and huge computational overhead, and hence cannot be directly applied on IoT-based E-commerce. In this paper, we propose NormaChain, a blockchain-based normalized autonomous transaction settlement system for IoT-based E-commerce. By designing a special three-layer sharding blockchain network, we can significantly increase transaction efficiency and system scalability. Additionally, by designing an innovative decentralized public key searchable encryption scheme (decentralized public key encryption with keyword search (PEKS) scheme), we can uncover illegal and criminal transactions and achieve crime traceability. Our new decentralized PEKS scheme cryptographically eliminates the dependence of a trusted central authority in the original PEKS scheme and instead expands it to a fully decentralized governance, which distributes the supervision power equally among all parties. More importantly, by proving NormaChain is secure against chosen ciphertext attacks and against the stealing of the secret key, we show that NormaChain prevents a legitimate user’s privacy from being violated by banks, supervisors or malicious adversaries. Finally, we deliver the NormaChain system with design details and full implementations. Experiments show that the average transaction-per-second on IoT devices is around 113, and the supervision accuracy is 100% when proper target illegal keywords are provided.
Nurse practitioner‐led surgical spine consultation clinic (2010)

Nurse practitioners can play an effective and efficient role in providing care to patients requiring specific disease management in a specialty setting. The nurse practitioner-run clinic offers accurate and earlier assessment, thus facilitating a timelier diagnosis and management plan.
Efficient Solid‐Phase Synthesis of pppRNA by Using Product‐Specific Labeling (2014)

A novel solid-phase synthesis and purification strategy for 5'-triphosphate oligonucleotides by using lipophilic tagging of the triphosphate moiety is reported. This is based on triphosphate synthesis with 5'-O-cyclotriphosphate intermediates, whereby a lipophilic tag, such as decylamine, is introduced during the ring-opening reaction to give a linear gamma-phosphate-tagged species. This method enables the highly efficient synthesis of 5'-triphosphorylated RNA derivatives and their gamma-phosphate-substituted analogues and will especially facilitate the advancement of therapeutic approaches that make use of 5'-triphosphate oligonucleotides as potent activators of the cytosolic immune sensor RIG-I.
Analysis of part motion on a longitudinally vibrating plate (2002)

We analyze the dynamics of part motion for a novel type of planar parts feeder consisting of a longitudinally vibrating flat plate and a part placed on its surface. For each vibration cycle, the plate's velocity is held positive (forward motion) for a longer time than it is held negative (backward motion). This type of asymmetric vibration combined with the nonlinear nature of Coulomb friction causes the part to accelerate along a straight line to a terminal velocity called the "feed rate". The average force exerted by the plate on the part is shown to be proportional to the latter's deviation from the feed rate. In other words, the part behaves as if it were immersed in a forward moving viscous fluid. Expressions for the feed rate and viscosity constants are derived with respect to various physical and control parameters. Rigid-body dynamic simulation results are shown to be in good agreement with the analysis.
A novel combination of TaqMan RT-PCR and a suspension microarray assay for the detection and species identification of pestiviruses (2009)

The genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. All are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. It affects both domestic pigs and wild boars. Outbreaks of this disease in domestic pigs call for the most serious measures of disease control, including a stamping out policy in Europe. Since all the members of the pestivirus genus can infect swine, differential diagnosis using traditional methods poses some problems. Antibody tests may lack specificity due to cross-reactions, antigen capture ELISAs may have low sensitivity, and virus isolation may take several days or even longer time to complete. PCR-based tests overcome these problems for the most part, but in general lack the multiplexing capability to detect and differentiate all the pestiviruses simultaneously. The assay platform described here addresses all of these issues by combining the advantages of real-time PCR with the multiplexing capability of microarray technology. The platform includes a TaqMan real-time PCR designed for the universal detection of pestiviruses and a microarray assay that can use the amplicons produced in the real-time PCR to identify the specific pestivirus.
Modifiable factors associated with a consistently high acute pain trajectory after surgical treatment of traumatic fractures in Ethiopia: a multi-center prospective cohort study (2023)

Higher preoperative pain and longer surgical time were associated with a consistently high acute postoperative pain trajectory. Clinicians may use these potentially modifiable factors to identify patients at risk for consistently high pain during the early postoperative period.
Impact of Acute Malaria on Pre-Existing Antibodies to Viral and Vaccine Antigens in Mice and Humans (2015)

Vaccine-induced immunity depends on long-lived plasma cells (LLPCs) that maintain antibody levels. A recent mouse study showed that Plasmodium chaubaudi infection reduced pre-existing influenza-specific antibodies--raising concerns that malaria may compromise pre-existing vaccine responses. We extended these findings to P. yoelii infection, observing decreases in antibodies to model antigens in inbred mice and to influenza in outbred mice, associated with LLPC depletion and increased susceptibility to influenza rechallenge. We investigated the implications of these findings in Malian children by measuring vaccine-specific IgG (tetanus, measles, hepatitis B) before and after the malaria-free 6-month dry season, 10 days after the first malaria episode of the malaria season, and after the subsequent dry season. On average, vaccine-specific IgG did not decrease following acute malaria. However, in some children malaria was associated with an accelerated decline in vaccine-specific IgG, underscoring the need to further investigate the impact of malaria on pre-existing vaccine-specific antibodies.
Noise reduction in GaN-based radio frequencysurface acoustic wave filters (2004)

In this work we have fabricated and characterized GaN based surface acoustic wave (SAW) delay lines grown by metal organic chemical vapor deposition (MOCVD) on sapphire substrate. The acoustic wave velocity of 0th Rayleigh and Sezawa modes, and the piezoelectric electromechanical coupling constant have been measured for different wave numbers in a 2μm-thick layer. The acoustic velocity resulted to be independent from the layer resistivity, which strongly affects the noise level. Through the introduction of a highly resistive GaN buffer layer, a noise level as low as −70dB has been measured. This result has been attributed to a reduced coupling between the input and output terminals.
Squirrels and nuts: metadata and knowledge management (2001)

There is a paradox in practical knowledge management in which the need to have an environment in which knowledge is organized in a more efficient manner needs to be balanced against the problem of “information overload”. Two approaches to resolving this paradox comprise: the “big software” approach, involving the use of a sophisticated search engine; and the “traditional information” approach, where content is organized beforehand using highly skilled people to spend time indexing, abstracting and isolating critical pieces of information within the bulk of any information source. It is proposed that a more practical approach would be to use techniques based on metadata, XML (Extensible Markup Language), tagging, data models and relational databases, arguing that, above a certain size of genuinely useful internal knowledge assets, the metadata approach is an essential management tool. While search engines performed reasonably well with the older, smaller databases, the increasing size of these databases means that size and density have become critical variables in knowledge management. Details of metadata and related techniques are described as a means of solving some of the problems involved in organizing knowledge in very large databases.
IL-2 regulation of soluble IL-2 receptor levels following thermal injury (1992)

In the immunosuppressed burn patient serum levels of both IL-2 and a soluble form of IL-2 receptor alpha (sIL-2R alpha) are significantly elevated. Strikingly, the production of these markers by the in vitro activated patients' cells is decreased. This study examines the role of IL-2 in the decreased production of the sIL-2R alpha in vitro in patients with major burns (n = 18, 30 to greater than 70% total body surface area). Peripheral blood mononuclear cell (PBMC) cultures from patients with highly elevated serum sIL-2R alpha, and from healthy controls (n = 12) were activated with concanavalin A (Con A) at initiation. In patients' cultures mitogen-induced increments of sIL-2R alpha levels were significantly lower. There was a significant negative correlation (r = 0.64, P less than 0.001) between a high serum sIL-2R alpha level and a decreased lectin-induced sIL-2R alpha release in vitro. Low levels of sIL-2R alpha in patients' samples were not normalized by increasing the number of T lymphocytes. Also exogenous rIL-1 was without effect, whereas rIL-3 increased sIL-2R alpha release in some cultures. However, sIL-2R alpha levels were significantly increased in patients' cultures by (i) addition of exogenous IL-2; (ii) removal of adherent cells; (iii) addition of cyclooxygenase inhibitor, indomethacin; (iv) bypassing cell surface activation by the combination of the calcium ionophore A23187 and the phorbol ester 12-o-tetradecanoyl acetate. The cyclic AMP-elevating drug, forskolin, abrogated the ability of exogenous IL-2 to increase sIL-2R alpha production. Thus, in the burn patient, the reduced in vitro sIL-2R alpha release appears to relate to abnormalities in IL-2 production and action mediated through its functional surface receptor. Elevated levels of sIL-2R alpha in vivo may, therefore, reflect systemic activation of T lymphocytes in response to biologically active IL-2.
EFFECT OF PROGESTINS ON GLUCOSE AND LIPID METABOLISM* (1977)

Gestamimetic amounts of progesterone enhance basal and glucose-stimulated insulin production. Contraceptive doses of synthetic progestins cause a moderate increase or no change in glucose-stimulated insulin production, depending on route of administration and species tested. Estrogens potentiate the insulinotropic effects of progesterone and the synthetic progestins. Basal serum triglyceride concentrations are generally unaffected by progesterone or 17 alpha-acetoxyprogesterone treatment but may decrease during 19-nortestosterone administration. Glucose tolerance does not change during treatment with gestamimetic doses of progesterone alone but may improve in rats and monkeys during concurrent estrogen administration. By contrast, deterioration of glucose tolerance is observed in women treated concurrently with synthetic estrogen plus 19-nortestosterone derivatives and, occasionally, with 19-nortestosterone derivatives alone. No consistent changes in glucose metabolism have been observed after treatment with 17 alpha-acetoxyprogesterone derivatives alone. The cause of the species-related differences in glucose metabolism during 19-nortestosterone treatment is obscure.
Risk of Solid Cancer After Treatment of Testicular Germ Cell Cancer in the Platinum Era (2018)

Purpose Testicular cancer (TC) treatment increases risk of subsequent malignant neoplasms (SMNs). It is unknown whether changes in TC treatment over time have affected SMN risk. Methods Solid SMN risk was evaluated in a multicenter cohort comprising 5,848 1-year survivors treated for TC before age 50 years between 1976 and 2007. SMN incidence was compared with cancer incidence in the general population. Treatment-specific risks were assessed using multivariable regression in a case-cohort design. Results After a median follow-up of 14.1 years, 350 solid SMNs were observed, translating into a 1.8-fold (95% CI, 1.6-2.0) increased risk compared with general population rates. Solid SMN risk was increased in patients with seminoma and those with nonseminoma (standardized incidence ratio, 1.52 and 2.21, respectively). Patients with nonseminoma experienced increased risk of SMNs of the thyroid, lung, stomach, pancreas, colon, and bladder and of melanoma and soft tissue sarcoma, whereas those with seminoma experienced increased risk of SMNs of the small intestine, pancreas, and urinary bladder. The 25-year cumulative incidence of solid SMNs was 10.3% (95% CI, 9.0% to 11.6%). In multivariable analysis, platinum-based chemotherapy was associated with increased risk of a solid SMN (hazard ratio [HR], 2.40; 95% CI, 1.58 to 3.62), colorectal SMN (HR, 3.85; 95% CI, 1.67 to 8.92), and noncolorectal GI SMN (HR, 5.00; 95% CI, 2.28 to 10.95). Receipt of platinum 400 to 499 and ≥ 500 mg/m 2 increased solid SMN risk compared with surgery only (HR, 2.43; 95% CI, 1.40 to 4.23 and HR, 2.42; 95% CI, 1.50 to 3.90, respectively), whereas risk was not significantly increased with lower doses (HR, 1.75; 95% CI, 0.90 to 3.43). The HR of a GI SMN increased by 53% (95% CI, 26% to 80%) per 100 mg/m 2 of platinum-containing chemotherapy. The HR of an infradiaphragmatic SMN increased by 8% per Gray of radiation dose administered (95% CI, 6% to 9%; P &lt; .001). Conclusion Radiotherapy and platinum-containing chemotherapy are associated with increased solid SMN risk, specifically with GI SMNs.
Use of the 'ex vivo' test to study long-term bacterial survival on human skin and their sensitivity to antisepsis (2004)

The apparent low bactericidal activity of biocides attributed in part to bacterial protection from skin layers is particularly important to assess in order to ensure antisepsis efficacy.
Enhanced uptake of potassium or glycine betaine or export of cyclic-di-AMP restores osmoresistance in a high cyclic-di-AMP Lactococcus lactis mutant (2018)

The broadly conserved bacterial signalling molecule cyclic-di-adenosine monophosphate (c-di-AMP) controls osmoresistance via its regulation of potassium (K+) and compatible solute uptake. High levels of c-di-AMP resulting from inactivation of c-di-AMP phosphodiesterase activity leads to poor growth of bacteria under high osmotic conditions. To better understand how bacteria can adjust in response to excessive c-di-AMP levels and to identify signals that feed into the c-di-AMP network, we characterised genes identified in a screen for osmoresistant suppressor mutants of the high c-di-AMP Lactococcus ΔgdpP strain. Mutations were identified which increased the uptake of osmoprotectants, including gain-of-function mutations in a Kup family K+ importer (KupB) and inactivation of the glycine betaine transporter transcriptional repressor BusR. The KupB mutations increased the intracellular K+ level while BusR inactivation increased the glycine betaine level. In addition, BusR was found to directly bind c-di-AMP and repress expression of the glycine betaine transporter in response to elevated c-di-AMP. Interestingly, overactive KupB activity or loss of BusR triggered c-di-AMP accumulation, suggesting turgor pressure changes act as a signal for this second messenger. In another group of suppressors, overexpression of an operon encoding an EmrB family multidrug resistance protein allowed cells to lower their intracellular level of c-di-AMP through active export. Lastly evidence is provided that c-di-AMP levels in several bacteria are rapidly responsive to environmental osmolarity changes. Taken together, this work provides evidence for a model in which high c-di-AMP containing cells are dehydrated due to lower K+ and compatible solute levels and that this osmoregulation system is able to sense and respond to cellular water stress.
Nano-scale silicon MOSFET: towards non-traditional and quantum devices (2002)

Nano-scale silicon MOSFETs with narrow wire channels on SOI substrates are presented. The triangular wire MOSFET can suppress the short channel effect more than double-gate and single-gate SOI MOSFETs. The wire channel MOSFETs narrower than 10 nm exhibit quantum confinement effects at room temperature. New device engineering using quantum effects is also discussed.
Premidnight plasmaspheric “plumes” (1997)

To explain observations of brief intervals of cold, dense plasma by geosynchronous satellites in the midnight sector prior to or during substorm onset, it has recently been proposed that dense plasmaspheric plasma is drawn out to geosynchronous orbit in the premidnight region by inductive electric fields during the growth phase of a geomagnetic substorm. We present here the results of a statistical study of such intervals observed with the Los Alamos magnetospheric plasma analyzer (MPA) on geosynchronous satellite 1989‐046 between March and December 1993. We find that these premidnight cold plasma intervals occur only after extended periods of low magnetospheric activity identified by K p and the midnight boundary index (MBI). We also find that the satellite typically enters the cold plasma region from the trough region and exits it into the plasma sheet. Finally, while measurements of the flow velocity of the cold plasma are rendered uncertain by the asymmetric spacecraft charge or photoelectron sheath, such measurements show no evidence of the outward flow that would be expected from the extrusion hypothesis. Rather, there are some indications that these cold plasma regions flow sunward in the (corotation) satellite frame. These results suggest an alternative explanation for the premidnight cold plasma: corotation‐dominated transport of day side plasmaspheric structures into the premidnight sector. The implications of our observations for the extrusion hypothesis and for the alternative explanation are discussed.
Effect of Nanoplatelets on the Rheological Behavior of Epoxy Monomers (2008)

Abstract The effect of nanoplatelets on the rheological behavior of epoxy monomers with variations in nanoplatelet exfoliation level and aspect ratio was investigated. The results show that the presence of exfoliated nanoplatelets in epoxy can significantly influence viscosity and lead to shear‐thinning phenomena, especially when the aspect ratio of the nanoplatelets is high. By employing the Krieger‐Dougherty model, the effectiveness of nanoplatelets in altering epoxy rheological behavior was quantitatively described and compared with experimental findings. Possible physical reasons for the observed rheological behaviors are discussed. The implication of the present study for polymer nanocomposites processing is also addressed. magnified image
STUDIES ON VITAL STAINING (1930)

When large amounts of brilliant vital red are injected into the blood stream of dogs, the dye is gradually removed from circulation, and most of it is deposited in numerous phagocytic cells which are scattered throughout various organs and tissues. The dye occurs largely in the form of tiny red granules crowded together in the cytoplasm of these cells. If Niagara sky blue, a closely related dyestuff, is injected, it too is taken up and stored in these same cells. It is shown that the presence of red dye in the tissues does not inhibit the cells from taking up the blue one. The normal ability of the phagocytes to take up Niagara sky blue is observed also when this dye is injected simultaneously with brilliant vital red. This normal response toward the blue dye is seen even though the phagocytes are busied at the same time in the process of engulfing and storing the red dye. These experiments show that it is difficult if not impossible to "block" the cells with one dye so that their ability to take up another is even slightly impaired. The two dyes employed in these studies are shown to be particularly suitable for experiments of the sort here reported.
Coronary calcification detected by electron-beam computed tomography and myocardial infarction. The Rotterdam Coronary Calcification Study (2002)

A strong and graded association was found between coronary calcification and myocardial infarction. The association remained at high ages.