gebhart/patrepeval huggingface dataset:
One-electron reactions of some mitochondrial components studied by pulse radiolysis (1970)

Research Article| February 01 1970 One-electron reactions of some mitochondrial components studied by pulse radiolysis E J Land; E J Land Search for other works by this author on: This Site PubMed Google Scholar A J Swallow A J Swallow Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1970) 116 (4): 16P. https://doi.org/10.1042/bj1160016Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation E J Land, A J Swallow; One-electron reactions of some mitochondrial components studied by pulse radiolysis. Biochem J 1 February 1970; 116 (4): 16P. doi: https://doi.org/10.1042/bj1160016Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1970 The Biochemical Society1970 Article PDF first page preview Close Modal You do not currently have access to this content.
MicroRNA-34c Enhances Murine Male Germ Cell Apoptosis through Targeting ATF1 (2012)

Our study shows for the first time that miR-34c functions, at least partially, by targeting the ATF1 gene in germ cell apoptosis, providing a novel mechanism with involvement of miRNA in the regulation of germ cell apoptosis.
Mapping Microvasculature with Acoustic Angiography Yields Quantifiable Differences between Healthy and Tumor-bearing Tissue Volumes in a Rodent Model (2020)

To determine if the morphologies of microvessels could be extracted from contrast material–enhanced acoustic angiographic ultrasonographic (US) images and used as a quantitative basis for distinguishing healthy from diseased tissue.
Bending Vibrator Consisting of a LiNbO3 Plate with a Ferroelectric Inversion Layer (1987)

An anti-polarity ferroelectric laminar domain (inversion layer) can be formed in a LiNbO 3 plate without applying any external fields by heat treatment at somewhat lower temperatures than the Curie point. A plate with such an inversion layer can be used as a bending vibrator with no bonding layer. This paper reports the characteristics of the bending vibrators. The 137° rotated Y -cut of LiNbO 3 is shown to be the optimum plate orientation for the bending vibrators because it exhibits a high transverse electromechanical coupling factor of about 0.51. It is also demonstrated that a bending vibrator fabricated using 128° rotated Y -cut LiNbO 3 has a capacitance ratio as low as 7.82, which is slightly higher than the theoretically predicted value.
On the reaction of bis(phosphothioyl)disul‐fanes with hydroxamic acids, part I: Ionic versus radical reaction pathways (2008)

Abstract Among sodium N ‐alkyl‐4‐chlorobenzo‐hydroxamates treated with bis(phosphothioyl)disul‐fanes &gt;P(S)SSP(S)&lt; (where &gt;P(S) is phosphorothioyl, phosphonothioyl, and phosphinothioyl), only the N ‐methyl one yields quantitatively the respective O ‐phosphothioyl derivatives exhibiting complete inversion of configuration at phosphorus in a reaction whose products are inert toward dithiophosphate arising in the reaction. For branched N ‐alkyl benzohydroxamates, products of a SET process predominated. The mechanism of the title reaction is discussed. © 2008 Wiley Periodicals, Inc. Heteroatom Chem 19:271–282, 2008; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/hc.20423
PEGylation of Membrane Proteins Like Bacteriorhodopsin as a Tool to Increase Their Stability toward Ethanol (2012)

Protection of biological compounds, for example, enzymes, viruses, or even whole cells, against degradation is very important for many applications. Embedding of such compounds into polymer matrices is a straightforward common method. However, in biotechnology and medicine there is a great interest to prepare micro- and nanosized shells around the biocomponents in order to protect them and having only a minor increase in size. The PEGylation of biological macromolecules has gained attention because degradation by proteolytic enzymes is significantly retarded and, in turn, their bioavailability is enhanced. We found that PEGylation is also a powerful tool to protect biomaterials from degradation by small organic solvent molecules, in particular, ethanol. Methoxy-polyethylene glycol (MPEG) modified BR survives exposure to significant concentrations of ethanol, up to 30%, and preserves its photochromism, whereas unmodified PM is instantaneously denatured at such concentrations. This is useful for potential technical applications of BR but is of relevance for many other applications where biomaterials and, in particular, biomembranes may be exposed to solvents.
Giant Excitonic Exchange Splittings at Zero Field in Single Colloidal CdSe Quantum Dots Doped with Individual Mn2+ Impurities (2016)

Replacing a single atom of a host semiconductor nanocrystal with a functional dopant can introduce completely new properties potentially valuable for "solotronic" information-processing applications. Here, we report successful doping of colloidal CdSe quantum dots with a very small number of manganese ions-down to the ultimate limit of one. Single-particle spectroscopy reveals spectral fingerprints of the spin-spin interactions between individual dopants and quantum-dot excitons. Spectrally well-resolved emission peaks are observed that can be related to the discrete spin projections of individual Mn<sup>2+</sup> ions. In agreement with theoretical predictions, the exchange splittings are enhanced by more than an order of magnitude in these quantum dots compared to their epitaxial counterparts, opening a path for solotronic applications at elevated temperatures.
Bottleneck Sharing Generative Adversarial Networks for Unified Multi-Contrast MR Image Synthesis (2022)

Magnetic Resonance Imaging (MRI) is the favored modality in multi-modal medical imaging due to its safety and ability to acquire various different contrasts of the anatomy. Availability of multiple contrasts accumulates diagnostic information and, therefore, can improve radiological observations. In some scenarios, acquiring all contrasts might be challenging due to reluctant patients and increased costs associated with additional scans. That said, synthetically obtaining missing MRI pulse sequences from the acquired sequences might prove to be useful for further analyses. Recently introduced Generative Adversarial Network (GAN) models offer state-of-the-art performance in learning MRI synthesis. However, the proposed generative approaches learn a distinct model for each conditional contrast to contrast mapping. Learning a distinct synthesis model for each individual task increases the time and memory demands due to the increased number of parameters and training time. To mitigate this issue, we propose a novel unified synthesis model, bottleneck sharing GAN (bsGAN), to consolidate learning of synthesis tasks in multi-contrast MRI. bsGAN comprises distinct convolutional encoders and decoders for each contrast to increase synthesis performance. A central information bottleneck is employed to distill hidden representations. The bottleneck, based on residual convolutional layers, is shared across contrasts to avoid introducing many learnable parameters. Qualitative and quantitative comparisons on a multi-contrast brain MRI dataset show the effectiveness of the proposed method against existing unified synthesis methods.
Kick Signatures Through Advanced Multi-Phase Data (2022)

Existing downhole geophysical sensors, like those used in LWD, offer an opportunity for early kick detection, but they are not currently used for detection or characterization. Downhole acoustic measurements also have the potential for providing reliable gas leak detection based on their high sensitivity to low concentrations of gas present in a gas-liquid mixture. This effort seeks to close a data knowledge gap through numerical simulation and use this data to aid in development of a low-cost, fast, and reliable early kick detection technology for preventing blowouts based on the acoustic signature.
Behaviour of compensation voltage in LCOS panels (2007)

Measurement data on the Vcom drift behaviour of LCOS panels with inorganic alignment layers is presented. Differences with polyimide coated cells are observed, as well as an influence of alignment layer thickness, the initial electrical field and the driving frequency on the evolution of Vcom with time.
The SMAC Mimetic AZD5582 is a Potent HIV Latency Reversing Agent (2018)

Abstract The leading strategy towards eradication of human immunodeficiency virus (HIV) infection is the depletion of viral reservoirs through reversal of viral latency, followed by clearance of persistently infected cells. To date, a latency reversing agent (LRA) that reactivates a majority of the quiescent provirus population, without significant off-target effects, has not been identified. We show here that molecules mimicking the active N-terminal tetrapeptide of the second mitochondrial-derived activator of caspases (SMACm) potently reverse HIV latency in vitro and ex vivo without the pleotropic cellular effects seen with other LRA. We verified that SMACm facilitate latency reversal through activation of the non-canonical NFkB pathway as exemplified by rapid degradation of cellular inhibitor of apoptosis protein 1 (cIAP1), followed by a slower conversion of the inactive p100 form of NFκB2 into the active p52 transcription factor. A potent representative of this class, AZD5582, increases cell-associated HIV RNA expression in resting CD4+ T cells from ART-suppressed, HIV-infected donors while altering the expression of a restricted number of human genes. These findings represent the first demonstration that SMACm have single agent latency reversal activity in patient-derived cells and support evaluation of this class of molecules in preclinical animal models.
Determination of Ductility for Bulk Deformation (1978)

The possibility of predicting the onset of fracture in bulk deformation processes from ductility parameters determined in simple tests was investigated by performing uniaxial tension, plane-strain tension, partial-width indentation, and secondary tension tests on 6061 aluminum, 1020 and 1045 steels, and lead. Limiting strains were correlated with those measured in upsetting with sticking at the end face and in rolling. Uniaxial tension was inapplicable, but the other tests showed reasonable agreement with a ductility criterion giving a ½ slope in a major (tensile) versus minor (compressive) strain plot, at least for isotropic materials. Normal anisotropy made the relationship invalid, with a much larger compressive or rolling strain attainable when the plastic strain ratio (r value) was less than unity.
Feasibility and clinical utility of cancer whole genome and transcriptome sequencing for pediatric and young adult solid tumors. (2021)

3063 Background: Next generation sequencing (NGS) assays have accelerated the identification of mutations and potential matched targeted therapies for patients with cancer. However, a significant proportion of patients do not derive clinical benefit from targeted panel sequencing approaches. Cancer whole genome and transcriptome sequencing (cWGTS) offers the opportunity to fully characterize tumors, but are challenged by significant cost and computational resource requirements, concerns of assay sensitivity, and the need to deliver curated results within clinically relevant time frames. We performed a prospective study to evaluate the feasibility and utility of cWGTS in pediatric and young adults with solid tumors. Methods: We developed an automated analytical workflow (Isabl) for the QC and processing of cWGTS data to include ensembl variant calling for germline and somatic substitutions, indels, and structural variants; fusion genes; gene expression; and mutation signatures. Treatment biomarkers were annotated using OncoKB with generation of a clinical prototype report. We tested the feasibility of cWGTS implementation, evaluated its analytical validity compared to standard diagnostic assays, and characterized the clinical utility of incremental findings in a prospective study of children and young adults treated at Memorial Sloan Kettering Cancer Center. Results: A total of 114 patients were enrolled. Standard NGS assays (MSK-IMPACT, MSK-Fusion) identified clinically relevant biomarkers in 22% of cases. The cWGTS process was completed, from sample acquisition to summary report, in less than 12 days. Comparison against clinically reported NGS results demonstrated high precision and recall for reported mutations (98.8%) with high concordance across variant allele representations (r 2 &gt; 0.73). cWGTS identified additional oncogenic mutations not captured by targeted sequencing in 49% of patients. Furthermore, incremental findings, beyond those identified by NGS assays, of direct clinical relevance (diagnostic, prognostic, therapy guiding) were identified in 26% of patients. Importantly, &lt; 5% of the incremental findings would have been captured by whole exome or transcriptome sequencing alone. Of possible therapeutic relevance, cWGTS analyses revealed a significantly higher tumor mutation burden than previously reported (range: 0 - 11.23). Conclusions: We demonstrate feasibility, analytical validity and clinical utility of cWGTS approaches in pediatric and young adult cancer patients, with nearly half of all patients having incremental findings that were not captured by standard targeted NGS approaches.
Electronic-Level Design of Stress-Corrosion Resistant Alloys: Quantum Steels (2002)

Abstract : As part of an integrated computational materials design effort, quantum mechanical calculations have predicted the roles of impurity and alloying elements in grain boundary cohesion to support design of hydrogen-resistant ultrahigh-strength steels. Predicted thermodynamic quantities have been integrated in systems design of new steels employing W, Re and B for enhanced cohesion. A prototype steel has demonstrated an ultimate tensile strength of 330 ksi with good toughness and ductility, while maintaining desired grain boundary composition.
Tantalacyclobutane Complexes Containing the Potentially C,N,N‘-Coordinating Ligand [C6H4(CH2N(Me)CH2CH2NMe2)-2]- (CNN) and Their Reactivity with Carbon Monoxide and tert-Butyl Isocyanide. X-ray Molecular Structures of [Ta{CH2CH(Me)CH2-1,3}(CNN)(O-t-Bu)2], [Ta{CH2CH(Ph)CH2-1,3}(CNN)(O-t-Bu)2], [Ta{C(O)((CH2)3-1,3)}(CNN)(O-t-Bu)2], and [Ta{C(N-t-Bu)CH2CH(Ph)CH2-1,4}(CNN)(O-t-Bu)2] (1997)

From the reactions of the unsubstituted tantalacyclobutane complex [Ta{(CH2)3-1,3}(CNN)(O-t-Bu)2] (CNN = [C6H4(CH2N(Me)CH2CH2NMe2)-2]-) (1) with propene and styrene have been isolated in good yields the substituted tantalacyclobutane complexes [Ta{CH2CH(Me)CH2-1,3}(CNN)(O-t-Bu)2] (2) and [Ta{CH2CH(Ph)CH2-1,3}(CNN)(O-t-Bu)2] (3), respectively. The X-ray molecular structures of 2 and 3 show them to be seven-coordinate pentagonal bipyramidal species in which the meridional ligation comprises C,N,N‘-bonding of the aryldiamine ligand CNN and σ-bonding of two carbons of the metallacycle; the two alkoxide groups occupy the apical positions. The organyl substituent of the metallacycle is positioned on the β-carbon. Complexes 2 and 3 can both be converted back into 1 by reacting them in Et2O with ethene. The reaction of 1 with carbon monoxide yields the oxytantalacyclopropane complex [Ta{C(O)((CH2)3-1,3)}(CNN)(O-t-Bu)2] (4), which has been isolated as an air-stable white solid in 59% yield. The X-ray molecular structure of 4 reveals that the carbon and oxygen atoms of the CO function are ligated in the meridional plane with further η3-C,N,N‘-ligation of CNN and the two alkoxides as found in 2 and 3. Reaction of the tantalacyclobutane complexes 2−4 with 1 equiv of t-BuNC affords the 1:1 insertion products [Ta{C(N-t-Bu)CH2CH(R)CH2-1,4}(CNN)(O-t-Bu)2] (R = H (5), Me (6), and Ph (7), respectively). Complexes 5 and 7 have been isolated as yellow solids (yields: 5, 62%; 7, 50%), while 6 has been prepared and characterized in situ. 1H and 13C NMR spectroscopy of 6 and 7 indicate for each complex the presence of two isomers in solution. The X-ray molecular structure of 7 shows η2-C,N-bonding of the CNN ligand through the aryl Cipso atom and the N(Me) donor atom and, most importantly, the presence of two tantalacyclic rings of different sizes that have a common Ta−C bond. The smaller three-membered ring comprises the metal center with the nitrogen and the α-carbon atom of the η2-bonded iminoacyl fragment, while the larger five-membered ring is a metallacylopentane system comprising the metal center and four carbon atoms.
Glycosylation alterations of cells in late phase apoptosis from colon carcinomas (1999)

Comparisons of carbohydrate profiles between control and apoptotic colon carcinoma cells were performed by flow cytometry using a set of lectins and anti-carbohydrate antibodies. The six cell lines analyzed presented distinct carbohydrate profiles before induction of apoptosis. PHA-L and MAA binding decreased after induction of apoptosis by UV-treatment. In contrast an increase of PNA binding was observed after induction of apoptosis, except on SW-48 cells for which a decrease occurred. A decrease of SNA binding was observed after induction of apoptosis from strongly positive control cell lines, whereas it increased on weakly positive ones. All the blood group related antigens A, H, Lewis a, Lewis x, Lewis b, and Lewis y, had their expression strongly diminished on apoptotic cells. These changes occurred irrespective of the mode of apoptosis induction since similar results were obtained after UV, TNFalpha, or anti-Fas treatment. Fucosyltransferases activities were also decreased after apoptosis induction, except for alpha1,3fucosyltransferase in anti-Fas treated HT-29 cells, where it was strongly augmented. This could be attributed to the IFNgamma preteatment required to induce Fas expression on these cells. Fucosidase activity decreased after induction of apoptosis suggesting that it was not responsible for the loss of fucosylated structures. In the rat PRO cell line, H blood group antigens are mainly carried by a high molecular weight variant of CD44. It could be shown that the loss of H antigen after induction of apoptosis correlated with a loss of the carrier glycoprotein.
G Protein-coupled Receptor Kinase-mediated Desensitization of Metabotropic Glutamate Receptor 1A Protects against Cell Death (2000)

Metabotropic glutamate receptors (mGluRs) constitute a unique subclass of G protein-coupled receptors (GPCRs) that bear little sequence homology to other members of the GPCR superfamily. The mGluR subtypes that are coupled to the hydrolysis of phosphoinositide contribute to both synaptic plasticity and glutamate-mediated excitotoxicity in neurons. In the present study, the expression of mGluR1a in HEK 293 cells led to agonist-independent cell death. Since G protein-coupled receptor kinases (GRKs) desensitize a diverse variety of GPCRs, we explored whether GRKs contributed to the regulation of both constitutive and agonist-stimulated mGluR1a activity and thereby may prevent mGluR1a-mediated excitotoxicity associated with mGluR1a overactivation. We find that the co-expression of mGluR1a with GRK2 and GRK5, but not GRK4 and GRK6, reduced both constitutive and agonist-stimulated mGluR1a activity. Agonist-stimulated mGluR1a phosphorylation was enhanced by the co-expression of GRK2 and was blocked by two different GRK2 dominant-negative mutants. Furthermore, GRK2-dependent mGluR1a desensitization protected against mGluR1a-mediated cell death, at least in part by blocking mGluR1a-stimulated apoptosis. Our data indicate that as with other members of the GPCR superfamily, a member of the structurally distinct mGluR family (mGluR1a) serves as a substrate for GRK-mediated phosphorylation and that GRK-dependent "feedback" modulation of mGluR1a responsiveness protects against pathophysiological mGluR1a signaling.
Analysis of major proteins and fat fractions associated with mare's milk fat globules (2008)

Although several studies have aimed to identify mare's milk proteins, only the major whey proteins and some caseins have yet been characterized. Incomplete sequencing of the equine genome and the difficulty of recovering highly hydrophobic proteins mean that little is known to date about the proteins associated with milk fat globules, which have been shown to play an important role in newborns' defense mechanisms. The fat fraction, in particular the distribution of unsaturated fatty acids, has been more extensively studied, but complex lipids are only partially elucidated. This study reports a 2-DE approach combined with a powerful method for de novo protein sequencing, and quali-quantitative data on complex lipid composition determined by high performance TLC (HPTLC) and GC. The presence in mare's milk of long-chain highly unsaturated fatty acids, and the evidence of close similarity between equine and human milk fat globule membrane proteins, support the use of mare's milk for human nutrition.
Figure S1 from Efficacy of AKT Inhibitor ARQ 092 Compared with Sorafenib in a Cirrhotic Rat Model with Hepatocellular Carcinoma (2023)

&lt;p&gt;Apoptosis&lt;/p&gt;
Urban land-cover classification based on swarm intelligence from high resolution remote sensing imagery (2011)

Urban land-cover classification is one of the most challenging problems in pattern analysis and machine intelligence systems in remote sensing. Dense urban environment sensed by very high-resolution (VHR) optical sensors is even more challenging. Occlusions and shadows due to buildings and trees hide some objects in the scene. Despite its simplicity and usefulness, conventional classification methods have failed to have a high classification accuracy in dense urban areas. The objective of this study is to improve the quality of the land-cover classification. We propose using a Particle Swarm Optimization (PSO) based algorithm for the classification of VHR (0.1 to 1 m) remote sensing data over urban areas. This method is to discover classification rules through simulating the social behavior of animals such as the behaviours of bird flocking. The results for aerial images classification show the significance of this method. PSO-based classifier has been applied to the classification of remote sensing data in dense urban district of Kitchener-Waterloo and has achieved high predictive accuracy of 90%.