gebhart/patrepeval huggingface dataset:
Comprehensive profiling of accessible surface glycans of mammalian sperm using a lectin microarray (2014)

It is well known that cell surface glycans or glycocalyx play important roles in sperm motility, maturation and fertilization. A comprehensive profile of the sperm surface glycans will greatly facilitate both basic research (sperm glycobiology) and clinical studies, such as diagnostics of infertility. As a group of natural glycan binders, lectin is an ideal tool for cell surface glycan profiling. However, because of the lack of effective technology, only a few lectins have been tested for lectin-sperm binding profiles. To address this challenge, we have developed a procedure for high-throughput probing of mammalian sperm with 91 lectins on lectin microarrays. Normal sperm from human, boar, bull, goat and rabbit were collected and analyzed on the lectin microarrays. Positive bindings of a set of ~50 lectins were observed for all the sperm of 5 species, which indicated a wide range of glycans are on the surface of mammalian sperm. Species specific lectin bindings were also observed. Clustering analysis revealed that the distances of the five species according to the lectin binding profiles are consistent with that of the genome sequence based phylogenetic tree except for rabbit. The procedure that we established in this study could be generally applicable for sperm from other species or defect sperm from the same species. We believe the lectin binding profiles of the mammalian sperm that we established in this study are valuable for both basic research and clinical studies.
Racial variations in the prevalence of primary open-angle glaucoma. The Baltimore Eye Survey. (1991)

--Black Americans are at higher risk of primary open-angle glaucoma than their white neighbors. This may reflect an underlying genetic susceptibility to this disease and indicates that additional efforts are needed to identify and treat this sight-threatening disorder in high-risk communities.
Peer-to-Peer Issue Tracking System: Challenges and Solutions (2008)

Issue tracking1 tools such as Jira or Bugzilla have become important for software development, especially with distributed development teams becoming the norm in several organizations. It is interesting to see that some open source software come with their own issue tracking systems, such as Eclipse bugzilla
Sprayed Microgels onto 2D and 3D scaffolds as drug eluting coatings (2008)

Big advances are being achieved in the design of new implantable devices. Coated scaffolds capable of releasing bioactive agents for inhibiting totally or partially the inflammatory response of the surrounding tissues, are now being regarded as potential useful systems. Polymeric nanoparticles are known to be able to provide a pro grammable and sustained local drug delivery [1-3]. In addition, biodegradable and biocompatible scaffolds having a highly open porous structure and good mechanical strength are needed to provide an optimal microenvironment for cell proliferation, migration, and differentiation, and guidance for cellular in-growth from host tissue [4]. This study describes preliminary results on a novel coat ing method using spraying techniques for coating micro-nanometric crosslinked hydrogels on 3D biodegradable scaffolds.
The New Role of Trade Unionism in the 21st Century: Lessons from Singapore (1997)

Abstract This paper examines the different types of ideological positions (welfarist, realist, and socialist) and, by extension, the roles that trade unions may adopt in the future. It suggests that a distinction can be made between what trade unions should be, by definition, and what they can be as we move into the 21st Century. It argues that the optimum role that trade unions can assume is one in which they are autonomous, yet choose to work in close partnership with a state which is dedicated to improving the welfare of the citizenry. The rationale for this argument is that a strong state is in a position to garner support for beneficial development programs, while autonomous trade unions can ensure that the interest of members are factored into state policies, even as they seek to enhance their voice and ownership at workplace level. The Singapore case is used as a template to support the arguments generated in this paper.
Vanillin derivative inhibits quorum sensing and biofilm formation in Pseudomonas aeruginosa: a study in a Caenorhabditis elegans infection model (2021)

Vanillin and its derivative, (4-((<i>E</i>)-(4-hydroxy-2-<b>m</b>ethylphenylimino) <b>m</b>ethyl)-2-methoxy<b>p</b>henol (MMP) were showed clear inhibition of violacein and pyocyanin at sub-MICs indicating a possible quorum quenching effect of both the compounds. MMP was able to inhibit the biofilm formation in <i>Pseudomonas aeruginosa</i> PAO1 at 125 μg/mL (<i>p <</i> 0.05), while vanillin at 250 μg/mL (<i>p <</i> 0.05) indicating that they act against quorum sensing regulated biofilm formation. The inhibition of biofilm was confirmed by visualization through fluorescence microscopy followed by docking analysis of molecules against quorum sensing activator proteins. Caenorhabditis <i>elegans</i> survival assay revealed that vanillin and MMP were able to increase survival of <i>C. elegans</i> from <i>P. aeruginosa</i> PAO1 infection. The study showed that the potent features of the MMP and vanillin in inhibiting the quorum sensing regulated virulence and biofilm, which was proved in <i>C. elegans</i> infection model as well as molecular docking studies.
Domain Names and Company Name Retrieval (1998)

Location of web information for particular companies based on their names has become an increasingly difficult problem as the Internet and the web grow.The use of a naming convention and the domain name system (DNS) for that purpose has caused complications for the latter while not solving the problem.While there have been several proposals to use contemporary, high-capability, directory service and search protocols to reduce the dependencies on DNS conventions, none of them have been significantly deployed.This document proposes a company name to URL mapping service based on the oldest and least complex of Internet directory protocols, whois, in order to explore whether an extremely simple and widely-deployed protocol can succeed where more complex and powerful options have failed or been excessively delayed.
Sub-millimeter resolution imaging through animal tissue ultrafast gated electronic holography (1991)

We have developed a new ultrafast optical technique for two-dimensional imaging of absorbing objects embedded in diffusing media that is well suited to imaging through human tissue. Preliminary experiments have demonstrated 0.1 mm resolution of submillimeter objects completely obscured by 6 mm of raw chicken muscle tissue. This, to our knowledge, is the highest spatial resolution ever reported in this type of application. We have also demonstrated the capability of imaging, with sub-millimeter resolution, objects buried in diffusing material several centimeters thick. The implications are that this technique is a good candidate for application to the problem of non-invasive imaging through human breast tissue for tumor screening.
CHO-DHFR cell line development platform: Application of Clonepix and Automated Mini Bioreactor (AMBR) technologies to meet accelerated timelines (2015)

Background The Holy Grail sought by all Bioprocess Cell Line Development (CLD) groups is achieving high yields from easilycultured, robustly-growing cells in timelines measured in weeks rather than months. As the first bottleneck in process development, CLD must first birth its product for upstream and downstream groups to initiate their own reproductive cycles. To facilitate shortened CLD timelines, scientists have turned to new technologies and automation platforms. Emerging high-throughput instrumentation such as Clonepix and Automated MicroBioreactors (AMBR) have been enthusiastically integrated into stable cell line generation platforms; however, application of these methodologies among users is divergent. In this study, we describe method development that is part of an ongoing effort to create a vigorous, adaptable, high-throughput cell line development platform, incorporating in-house VRC-DG44-CHO cells, expression vectors with high-activity regulatory elements, and efficient selection and evaluation methods that result in early generation of optimal-yielding, vigorous, stable cell lines. This study was aimed toward a) evaluating early incorporation of Clonepix technology to maximize transfectant pool heterogeneity, b) identifying Clonepix fluorescence attributes that increase the probability of high-productivity clone isolation, c) evaluating and optimizing AMBR operating conditions to achieve process attributes comparable to and predictive of medium-scale bioreactors, and implementation of these practices for maximal results with shortened timelines.
Image force effects on carrier collection in a-Si:H solar cells (1981)

A modified carrier collection model of a-Si:H Schottky-barrier solar cells is proposed to explain the falloff of short wavelength carrier collection efficiency. Schottky-barrier lowering due to image force effects alters the potential distribution near the metal-semiconductor interface. This altered potential decreases the carrier collection in a-Si:H solar cells by as much as 30%. Design considerations to improve carrier collection efficiency are also proposed.
The Dynamoor Experiment (1993)

Abstract : Under the sponsorship of the Office of Naval Research, the Ocean Systems and Moorings Laboratory (OS and M Lab) of the Woods Hole Oceanographic Institution developed (1990) and deployed (1991) a fully instrumented subsurface buoy in high current waters close to Woods Hole, MA. The purpose of this engineering experiment, named DYNAMOOR, was to obtain long term, high frequency measurements of the buoy 3D position and of the tension in its mooring line, as a function of prevailing currents and variable, adjustable buoyancy. This paper first describes the main components and the method of deployment of the DYNAMOOR complex experimental setup. It then reviews the mechanical and electrical designs of the entire system. Finally, a review of the data analysis and a summary of the results are presented.
ChemInform Abstract: Entilin D (I), a Heptanortriterpenoid from the Bark of Entandrophragma utile. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The Effect of Complete Blood Count Timing on Lumbar Puncture Rates in Asymptomatic Infants Born to Mothers with Chorioamnionitis (2018)

Background Maternal chorioamnionitis is a risk factor for sepsis but, often, these infants are asymptomatic at birth. Different markers for infections, such as the immature to total (I/T) white blood cell (WBC) ratio, are used to help determine which infants require lumbar punctures (LPs), in addition to blood cultures and antibiotics. The timing of when the complete blood count (CBC) is obtained may have some effect on the length of antibiotic treatment. Aims The purpose of this proof-of-concept study was to assess if obtaining a CBC at greater than four hours of life as compared to less than four hours of life has an impact on the incidence of LPs performed in asymptomatic, full-term infants undergoing evaluation for sepsis secondary to maternal chorioamnionitis. Methods We performed a retrospective study of full-term, asymptomatic infants admitted for sepsis evaluation secondary to maternal chorioamnionitis. Subjects were grouped based upon the timing of their initial CBC (early = < four hours of life or late = > four hours of life). The incidence of LPs, duration of antibiotic treatment, and length of hospitalization were compared between the groups. Results A total of 230 subjects were included in the study (early group = 124, late group = 106). Subjects in the late group underwent significantly fewer LPs than subjects in the early group, 5.7% vs. 22.6% (p<0.001). There was no difference in length of treatment or hospitalization. Conclusions Asymptomatic full-term infants undergoing evaluation for sepsis secondary to maternal chorioamnionitis are less likely to undergo an LP if their initial CBC is obtained at greater than four hours of life.
Photochemical generation of a reactive transition-metal fragment. Photochemically induced arene replacement reactions of the cyclopentadienyl(p-xylene)iron(II) ion (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemical generation of a reactive transition-metal fragment. Photochemically induced arene replacement reactions of the cyclopentadienyl(p-xylene)iron(II) ionThomas P. Gill and Kent R. MannCite this: Inorg. Chem. 1980, 19, 10, 3007–3010Publication Date (Print):October 1, 1980Publication History Published online1 May 2002Published inissue 1 October 1980https://pubs.acs.org/doi/10.1021/ic50212a033https://doi.org/10.1021/ic50212a033research-articleACS PublicationsRequest reuse permissionsArticle Views582Altmetric-Citations104LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cultivation of Anaerobic and Facultatively Anaerobic Bacteria from Spacecraft-Associated Clean Rooms (2009)

In the course of this biodiversity study, the cultivable microbial community of European spacecraft-associated clean rooms and the Herschel Space Observatory located therein were analyzed during routine assembly operations. Here, we focused on microorganisms capable of growing without oxygen. Anaerobes play a significant role in planetary protection considerations since extraterrestrial environments like Mars probably do not provide enough oxygen for fully aerobic microbial growth. A broad assortment of anaerobic media was used in our cultivation strategies, which focused on microorganisms with special metabolic skills. The majority of the isolated strains grew on anaerobic, complex, nutrient-rich media. Autotrophic microorganisms or microbes capable of fixing nitrogen were also cultivated. A broad range of facultatively anaerobic bacteria was detected during this study and also, for the first time, some strictly anaerobic bacteria (Clostridium and Propionibacterium) were isolated from spacecraft-associated clean rooms. The multiassay cultivation approach was the basis for the detection of several bacteria that had not been cultivated from these special environments before and also led to the discovery of two novel microbial species of Pseudomonas and Paenibacillus.
Comparison of turbulent drop breakup in an emulsification device and homogeneous isotropic turbulence: Insights from numerical experiments (2022)

Turbulent emulsification is of considerable industrial interest. Nevertheless, numerical experiments (direct numerical simulations, DNS, with highly resolved interface tracking) have been mainly used to study drop breakup in idealized flows. This study, therefore, compares drop breakup in two different settings (homogenous and isotropic flow, and a simplified high-pressure homogenizer) with the intention of better understanding how insight gained from the idealized systems can be applied to industrially relevant devices. The flow differs between the two cases, with highly anisotropic and inhomogeneous turbulence in the latter. Results show similarities between the two cases regarding morphology of breakup, suggesting that the underlying mechanism, as a function of Weber number, is similar. However, differences are also observed, e.g., in terms of breakup time and deformed morphology, which are associated with the locality of the turbulence in the homogenizer. Implications for an improved understanding of turbulent breakup in industrially relevant devices are discussed.
New coordination modes at molybdenum for 2-diphenylphosphinoaniline derived ligands (2007)

The complexes [MoCl3(1-N,2-Ph2P-C6H4)2] (1) and {MoCl(Nt-Bu)[1-micro(N),2-(Ph2P)C6H4]}2 (2) have been obtained from the reaction of 2-diphenylphosphinoaniline [1,2(NH2)(Ph2P)C6H4] with either sodium molybdate [Na2MoO4] (in the presence of Et3N and Me3SiCl) or [MoCl2(Nt-Bu)2(DME)]; the crystal structure of 1 reveals a novel MoNC2PN six-membered conjugated ring system derived from a P-N coupling reaction between two ligands, whilst that of 2 reveals bridging imido/terminal phosphine ligation.
[A questionnaire report concerning the actual situation of respiratory physicians in Japan]. (2006)

A nation-wide questionnaire was carried out at the end of 2004 to elucidate the actual situation of respiratory physicians in Japan. The questionnaires were characterized by simultaneously looking into the actual situation in the departments of respiratory medicine, cardiology and gastroenterology. The number of the surveyed institution was 168, from which questionnaire replies were obtained from 79 general hospitals, 39 university hospitals and 10 hospitals mainly for tuberculosis. In general hospitals, compared to the departments of cardiology and gastroenterology, the full-time physician staff in the department of respiratory medicine per 10 inpatient beds was only 60.9% and 79.4%, while specialist staff were 54.9% and 68.4%, respectively. The numbers of deaths in the department of respiratory medicine per 10 inpatient beds were 186.9% and 132.6%, compared with in the departments of cardiology and gastroenterology, while those per full-time physicians were 271.9% and 148.5%, respectively. Thus, it was concluded that there was an obvious shortage in number of not only in respiratory specialists but also of all respiratory physicians. Furthermore, respiratory physicians were taking care of more severe patients than other colleagues.
Bioactive 11,20-Epoxy-3,5(16)-diene Briarane Diterpenoids from the South China Sea Gorgonian Dichotella gemmacea (2011)

Seven new briarane diterpenoids, gemmacolides G–M (1–7), were isolated together with two known analogues, juncin O and junceellolide C, from the South China Sea gorgonian Dichotella gemmacea. The structures of the new compounds were elucidated by detailed analysis of spectroscopic data and comparison with reported data. In an in vitro bioassay, these compounds exhibited different levels of growth inhibition activity against A549 and MG63 cells. In particular, compound 4 was more active than the positive control adriamycin against A549 cells. Compounds 4 and 7 also exhibited weak antimicrobial activity against the bacterium Bacillus megaterium and the fungus Septoria tritici, respectively.
Investigation of Chemical Generation of Atomic Iodine for COIL, and its Testing in the Supersonic COIL, Using a Diode Probe (2004)

The research on atomic iodine generation in the framework of this grant follows-up the previous contracts but the experimental work has moved up in the case of "chlorine" system from the small-scale devices to the supersonic COIL. The Iodine Scan Laser Diode Probe diagnostics was used for the parametric COIL testing. This diagnostics lent to our COIL Laboratory from the US AFRL at the Kirtland AFB was necessary for this investigation.
Nucleosome landscape in HepG2 cells in relation to NFY, HNF4a and FOXA2 binding sites (2010)

The key to the complexity of higher organisms lies not in the number of protein coding genes they carry, but rather in the intrinsic complexity of the gene regulatory networks. The major effectors of transcriptional regulation are proteins called transcription factors, and in this thesis four papers describing genome-wide studies of seven such factors are presented, together with studies on components of the chromatin and transcriptome. In Paper I, we optimized a large-scale in vivo method, ChIP-chip, to study protein – DNA interactions using microarrays. The metabolic-disease related transcription factors USF1, HNF4a and FOXA2 were studied in 1 % of the genome, and a surprising number of binding sites were found, mostly far from annotated genes. In Paper II, a novel sequencing based method, ChIP-seq, was applied to FOXA2, HNF4a and GABPa, allowing a true genome-wide view of binding sites. A large overlap between the datasets were seen, and molecular interactions were verified in vivo. Using a ChIP-seq specific motif discovery method, we identified both the expected motifs and several for co-localized transcription factors. In Paper III, we identified and studied a novel transcription factor, ZBED6, using the ChIP-seq method. Here, we went from one known binding site to several hundred sites throughout the mouse genome. Finally, in Paper IV, we studied the chromatin landscape by deep sequencing of nucleosomal DNA, and further used RNA-sequencing to quantify expression levels, and extended the knowledge about the binding profiles for the transcription factors NFY and TCF7L2.
Theory for the extension of a linear polyelectrolyte attached at one end in an electric field (1990)

Abstract A simple theory is developed for the extension of a uniformly charged linear polyion attached at one end in an electric field. For a polyion consisting of a very large number ( N ) of Kuhn lengths ( b ), the mean extension in the direction of the electric field ( E ) is given accurately by R z = ( b / A ) In (sinh ( NA )/ NA ), where A = EQb / k B T and Q is the effective charge associated with each Kuhn length. For any value of E , no matter how small, a polyion of sufficient length, such that NA ≫ 1.0, will be essentially fully extended. When A ≪ 1.0, as is the case for DNA in weak electric fields, the head end of the chain is only weakly oriented, even though the stem and tail may be completely aligned. In the linear regime, NA &lt; 1.0, R z is proportional to E, Q , and N 2 . The linear density of chain elements is calculated for a chain in the linear regime. This theory provides a basis to determine the product Qb from direct microscopic measurements of R z vs. E for fluorescent‐labeled circular DNAs that are topologically snared near voids or surfaces of gels. Experimental realization of this suggestion is described in the following paper.