gebhart/patrepeval huggingface dataset:
A systematic search for DNA methyltransferase polymorphisms reveals a rare DNMT3L variant associated with subtelomeric hypomethylation (2009)

Causes underlying inter-individual variations in DNA methylation profiles among normal healthy populations are not thoroughly understood. To investigate the contribution of genetic variation in DNA methyltransferase (DNMT) genes to such epigenetic variation, we performed a systematic search for polymorphisms in all known human DNMT genes [DNMT1, DNMT3A, DNMT3B, DNMT3L and DNMT2 (TRDMT1)] in 192 healthy males and females. One hundred and eleven different polymorphisms were detected. Of these, 24 were located in coding regions and 10 resulted in an amino acid change that may affect the corresponding DNMT protein structure or function. Association analysis between all major polymorphisms (frequency > 1%) and quantitative DNA methylation profiles did not return significant results after correction for multiple testing. Polymorphisms leading to an amino acid change were further investigated for changes in global DNA methylation by differential methylation hybridization. This analysis revealed that a rare change at DNMT3L (R271Q) was associated with significant DNA hypomethylation. Biochemical characterization confirmed that DNMT3L(R271Q) is impaired in its ability to stimulate de novo DNA methylation by DNMT3A. Methylated DNA immunoprecipitation based analysis using CpG island microarrays revealed that the hypomethylation in this sample preferentially clustered to subtelomeric genomic regions with affected loci corresponding to a subset of repetitive CpG islands with low predicted promoter potential located outside of genes.
Managing the Costs of Healthcare for an Aging Population: The Fiscal Impact of New Brunswick’s Demographic Glacier (2015)

Social Policy, Health Policy, Healthcare, Seniors
Time-Dependent Quantitative Multicomponent Control of the G 1 -S Network by the Stress-Activated Protein Kinase Hog1 upon Osmostress (2011)

Control of cell cycle progression by stress-activated protein kinases (SAPKs) is essential for cell adaptation to extracellular stimuli. Exposure of yeast to hyperosmotic stress activates the SAPK Hog1, which delays cell cycle progression through G₁ by direct phosphorylation of the cyclin-dependent kinase (CDK) inhibitor Sic1 and by inhibition of the transcription of the genes encoding the G₁ cyclins Cln1 and 2. Additional targets of Hog1 may also play a role in this response. We used mathematical modeling and quantitative in vivo experiments to define the contributions of individual components of the G₁-S network downstream of Hog1 to this stress-induced delay in the cell cycle. The length of the arrest depended on the degree of stress and the temporal proximity of the onset of the stress to the commitment to cell division, called "Start." Hog1-induced inhibition of the transcription of the gene encoding cyclin Clb5, rather than that of the gene encoding Cln2, prevented entry into S phase upon osmostress. By controlling the accumulation of specific cyclins, Hog1 delayed bud morphogenesis (through Clns) and delayed DNA replication (through Clb5). Hog1-mediated phosphorylation and degradation of Sic1 at Start prevented residual activity of the cyclin/CDK complex Clb5/Cdc28 from initiating DNA replication before adaptation to the stress. Thus, our work defines distinct temporal roles for the actions of Hog1 on Sic1 and cyclins in mediating G₁ arrest upon hyperosmotic stress.
Poly(glycerol sebacate) nanoparticles for encapsulation of hydrophobic anti-cancer drugs (2017)

Physical encapsulation of hydrophobic compounds into nanocarriers that are stable in aqueous medium is of high interest as it can increase solubilization of the drug, lower its toxicity, control its pharmacokinetic profile and thus overall improve the therapeutic efficacy.
Continuous Frequency-Sweep Covering Normal Direction Using Spoof Plasmonic Waveguide (2019)

Spoof surface plasmon polaritons are mimicing surface plasmon polaritons (SPPs) modes at microwave or terahertz frequencies based on periodic metamaterial. The SPP mode has been widely used in novel transmission waveguides and functional devices, due to its unique properties of great confinement, shorter operating wavelength, low loss, and ability in flexible transmission. A frequency-sweep radiation based on complementary SSPP transmission line is proposed, in which the frequency-scanning on both sides of the transmission line is achieved. Additional array of circular patches is introduced to overcome the disadvantage of low-radiation-efficiency in the normal direction. Leaky-wave radiation within wide-range from backward direction to forward direction is achieved.
A Resist Material Study for LWR and Resolution Improvement in EUV Lithography (2008)

It had been reported that LWR and resolution were improved according to loading amount of quencher along with a decrease of sensitivity. Influences of quencher and PAG anion size on acid diffusion in acetal protected PHS-based polymer were examined and it was found that acid diffusion length is mainly controlled by quencher, resulting in the improvement of LWR and resolution as well as the decrease of sensitivity by way of reducing acid diffusion length. In order to compensate for the sensitivity loss, we investigated the effect of PAG structures and properties on acid generation and it was found that acid generation efficiency increased with the reduction potential of PAG. In this paper, we discussed how to solve the dilemma among sensitivity, resolution and LWR in EUV lithography, by applying high-efficiency PAG under high quencher concentration.
Method for Long-term Estimating Ice Algae Biomass Based on Spectral Attenuation Characteristics of the Sea Ice (2019)

Ice algae plays an important role in the polar marine ecosystem, so it is essential to estimate the biomass of polar ice algae. In this paper, we measured the internal irradiance of sea ice and calculated the spectral attenuation characteristics of sea ice media. We used Empirical Orthogonal Function(EOF) analysis to reduce the dimension of data, extract the characteristics of spectral attenuation coefficient, and established the relationship between chlorophyll a concentration and spectral attenuation coefficient through Generalized Linear Model. A method for estimating the biomass of polar ice algae represented by chlorophyll a concentration was proposed.
Altered Expression of MicroRNAs in Rheumatoid Arthritis (2017)

Rheumatoid arthritis is one of the most common types of inflammatory joint diseases. Women, smokers, and people with positive family history are more susceptible to this disease. Diagnostic criteria include at least one swollen joint that has not been caused by other diseases. MicroRNAs are non-coding RNAs that are evolutionarily conserved and have a length of 18-25 nucleotides. MicroRNAs control gene expression at the post-transcriptional level via promoting mRNA degradation or translational repression. Recognition of alterations in microRNA status and their respective targets, may offer an opportunity to better identify the pathways that are involved in the etiopathogenesis of autoimmune diseases. It has been suggested that microRNAs may serve as potential biomarkers for both diagnosis and prognosis of autoimmune diseases. Here, we review the available evidence on the deregulations of microRNA expression in rheumatoid arthritis. More precisely, this review focuses on the microRNA involved in T cell regulation and gives perspectives on the use of this microRNA as biomarkers of diagnosis, prognosis, or intervention efficacy. J. Cell. Biochem. 119: 478-487, 2018. © 2017 Wiley Periodicals, Inc.
Fixation of bioprosthetic tissues with monofunctional and multifunctional polyepoxy compounds (1994)

Collagen from a native tissue is fixed with a polyepoxy compound (PC) for use as a new biologic prosthetic material. Prior studies have shown that this biomaterial has comparable properties with collagen fixed with glutaraldehyde (GA), and thus has great promise for biomedical applications. A prior kinetic study indicated that the reaction between the functional groups of collagen and the multifunctional epoxy EX-313 is a 2.5th-order reaction. The purpose of this study was to understand the mechanism of the amino acid-PC reactions in a fixation process. Bovine arteries were fixed with a monofunctional PC (EX-131) and a multifunctional PC (EX-313) as a function of fixation time. A sequential fixation with a second fixative was used to identify the available remaining reactive sites from a prior fixation. The denaturation temperature (Td) was measured on each sample. Because the denaturation temperature is a direct indication of crosslinking of individual amino acids with the fixative, the increase in Td of a subsequent fixation may be indicative of the available remaining amino acids. The fixation index was measured on each sample to reflect the increase of fixation completion in a sequential fixation process. The fixation index and crosslink data also revealed that the reactive amino acids for EX-131 and EX-313 may not be exactly the same. The data in this study suggest that a monofunctional fixative can pre-react with the amino acids of collagen to effectively block further fixation of collagen with a second fixative. This amino acid masking may be associated with collagen branching. Collagen branching and its effect on denaturation temperature are described.
Rhodium and Iridium Fluorido and Bifluorido Complexes Derived from Peroxido Precursors (2016)

The rhodium and iridium fluorido bifluorido complexes trans ‐[Rh(4‐C 5 F 4 N)(F)(FHF)(CN t Bu)(PEt 3 ) 2 ] ( 1 ) and trans ‐[Ir(4‐C 5 F 4 N)(F)(FHF)(CN t Bu)(P i Pr 3 ) 2 ] ( 2 ) have been synthesized by protonation of the peroxido complexes trans ‐[M(4‐C 5 F 4 N)(O 2 )(CN t Bu)(PR 3 ) 2 ] with an HF source (M = Rh, R = Et; M = Ir, R = i Pr). Abstraction of the coordinated HF resulted in the formation of the difluorido compounds trans ‐[M(4‐C 5 F 4 N)(F) 2 (CN t Bu)(PR 3 ) 2 ] ( 4 : M = Rh, R = Et; 5 : M = Ir, R = i Pr).
Accurate Live Interfacial Tension for Improved Reservoir Engineering Practices (2021)

Abstract Current reservoir simulators use interfacial tension (IFT) values derived from dead oil measurements at ambient conditions or predicted from literature correlations. IFT is highly dependent on temperature, pressure and fluid composition. Therefore, knowledge of the IFT value at reservoir conditions is essential for accurate reservoir fluid characterization. This study compares IFT values from dead and live oil measurements and the results of literature predicted values, thereby clearly showing the weakness of existing correlations when trying to predict crude oil IFT. A total of ten live oils was sampled for this study. Using the pendent drop technique, IFT was measured for each oil at different conditions: in the under-saturated region at reservoir pressure and temperature, in the saturated region at reservoir temperature, and for dead oil at ambient conditions. Basic PVT properties such as gas to oil ratio (GOR), gas and liquid composition, density, viscosity and molecular weight were also measured. The bubble point for each oil was identified to define the pressure step in the saturated region for extra IFT measurement. The equilibrium IFT values for the live oils were generally higher than for the corresponding dead oils. For oils where this general trend was not observed, contaminations were found in the crude samples. The use of current literature correlations does not allow to predict correct reservoir IFT. Therefore, this study provides accurate live IFT values for a variety of reservoir fluids and conditions in combination with live oil properties, highly beneficial to reservoir engineers, allowing better oil production planning.
Building a 3-D Line-Based Map Using Stereo SLAM (2015)

This paper presents a graph-based visual simultaneous localization and mapping (SLAM) system using straight lines as features. Compared with point features, lines provide far richer information about the structure of the environment and make it possible to infer spatial semantics from the map. Using a stereo rig as the sole sensor, our proposed system utilizes many advanced techniques, such as motion estimation, pose optimization, and bundle adjustment. We use two different representations to parameterize 3-D lines in this paper: Plücker line coordinates for efficient initialization of newly observed line features and projection of 3-D lines, and orthonormal representation for graph optimization. The proposed system is tested with indoor and outdoor sequences, and it exhibits better reconstruction performance against a point-based SLAM system in line-rich environments.
ChemInform Abstract: KINETIK DER NITRIERUNG VON 4‐(2,4‐DINITROPHENYL)‐1‐METHYL‐PYRAZOL (1971)

Abstract Die Kinetik der Nitrierung von (I) zu (II) und (III) wird untersucht.
PhdA Catalyzes the First Step of Phenazine-1-Carboxylic Acid Degradation in Mycobacterium fortuitum (2018)

Phenazines are a class of bacterially produced redox-active metabolites that are found in natural, industrial, and clinical environments. In <i>Pseudomonas</i> spp., phenazine-1-carboxylic acid (PCA)-the precursor of all phenazine metabolites-facilitates nutrient acquisition, biofilm formation, and competition with other organisms. While the removal of phenazines negatively impacts these activities, little is known about the genes or enzymes responsible for phenazine degradation by other organisms. Here, we report that the first step of PCA degradation by <i>Mycobacterium fortuitum</i> is catalyzed by a <u>ph</u>enazine-<u>d</u>egrading decarboxylase (PhdA). PhdA is related to members of the UbiD protein family that rely on a prenylated flavin mononucleotide cofactor for activity. The gene for PhdB, the enzyme responsible for cofactor synthesis, is present in a putative operon with the gene encoding PhdA in a region of the <i>M. fortuitum</i> genome that is essential for PCA degradation. PhdA and PhdB are present in all known PCA-degrading organisms from the <i>Actinobacteria</i><i>M. fortuitum</i> can also catabolize other <i>Pseudomonas</i>-derived phenazines such as phenazine-1-carboxamide, 1-hydroxyphenazine, and pyocyanin. On the basis of our previous work and the current characterization of PhdA, we propose that degradation converges on a common intermediate: dihydroxyphenazine. An understanding of the genes responsible for degradation will enable targeted studies of phenazine degraders in diverse environments.<b>IMPORTANCE</b> Bacteria from phylogenetically diverse groups secrete redox-active metabolites that provide a fitness advantage for their producers. For example, phenazines from <i>Pseudomonas</i> spp. benefit the producers by facilitating anoxic survival and biofilm formation and additionally inhibit competitors by serving as antimicrobials. Phenazine-producing pseudomonads act as biocontrol agents by leveraging these antibiotic properties to inhibit plant pests. Despite this importance, the fate of phenazines in the environment is poorly understood. Here, we characterize an enzyme from <i>Mycobacterium fortuitum</i> that catalyzes the first step of phenazine-1-carboxylic acid degradation. Knowledge of the genetic basis of phenazine degradation will facilitate the identification of environments where this activity influences the microbial community structure.
[Extra-membranous glomerulonephritis: report of a family and new physiopathological concepts]. (2003)

Membranous glomerulonephritis (MGN) is a major cause of the nephrotic syndrome and chronic renal insufficiency. Studies on the reactivity of nephritogenic antibodies implicated in a case of antenatal MGN led to identify for the first time a target of the immune conflict in glomeruli, neutral endopeptidase (CD10). This enzyme that is restricted to certain tissues, being strongly expressed in the placenta and on glomerular podocytes, is involved in the catabolism of a number of regulatory peptides, particularly those involved in vasomotricity. We showed that the nephritogenic antibodies blocked enzymatic activity and that the mother became immunized at the time of a previous pregnancy because she was neutral endopeptidase-deficient. Two additional families have been identified since then. The pathophysiologic mechanisms that we have unraveled are reminiscent of Rhesus immunization and open up new avenues for therapeutic intervention.
Combined Lane Mapping Using a Mobile Mapping System (2019)

High-definition mapping of 3D lane lines has been widely needed for the highway documentation and intelligent navigation of autonomous systems. A mobile mapping system (MMS) captures both accurate 3D LiDAR point clouds and high-resolution images of lane markings at highway driving speeds, providing an abundant data source for combined lane mapping. This paper aims to map lanes with an MMS. The main contributions of this paper include the following: (1) an intensity correction method was introduced to eliminate the reflectivity inconsistency of road-surface LiDAR points; (2) a self-adaptive thresholding method was developed to extract lane markings from their complicated surroundings; and (3) a LiDAR-guided textural saliency analysis of MMS images was proposed to improve the robustness of lane mapping. The proposed method was tested with a dataset acquired in Wuhan, Hubei, China, which contained straight roads, curved roads, and a roundabout with various pavement markings and a complex roadside environment. The experimental results achieved a recall of 96.4%, a precision of 97.6%, and an F-score of 97.0%, demonstrating that the proposed method has strong mapping ability for various urban roads.
Spatial organization of protein export in malaria parasite blood stages (2018)

Plasmodium falciparum, which causes malaria, extensively remodels its human host cells, particularly erythrocytes. Remodelling is essential for parasite survival by helping to avoid host immunity and assisting in the uptake of plasma nutrients to fuel rapid growth. Host cell renovation is carried out by hundreds of parasite effector proteins that are exported into the erythrocyte across an enveloping parasitophorous vacuole membrane (PVM). The Plasmodium translocon for exported (PTEX) proteins is thought to span the PVM and provide a channel that unfolds and extrudes proteins across the PVM into the erythrocyte. We show that exported reporter proteins containing mouse dihydrofolate reductase domains that inducibly resist unfolding become trapped at the parasite surface partly colocalizing with PTEX. When cargo is trapped, loop-like extensions appear at the PVM containing both trapped cargo and PTEX protein EXP2, but not additional components HSP101 and PTEX150. Following removal of the block-inducing compound, export of reporter proteins only partly recovers possibly because much of the trapped cargo is spatially segregated in the loop regions away from PTEX. This suggests that parasites have the means to isolate unfoldable cargo proteins from PTEX-containing export zones to avert disruption of protein export that would reduce parasite growth.
AC loss Characteristics under Critical Current Degradation of HTS Tapes (2005)

Critical current degradation of High Superconducting(HTS) tapes and AC loss under mechanical load is one of the hottest issues in HTS development and application. Mechanical load reduces the critical current of superconducting wire, and the degradation affects the AC loss of the wire. We measured the degradation and AC loss under tension and bending of Bi-2223 tapes made by 'Powder-in-Tube' technique at 77K with self-field. Also, we have studied the frequency characteristics on self-field AC loss in multi-filamentary Bi-2223/Ag tape at 77K. The measurement results and discussions on the relationship between degradation and AC loss are presented.
Fourier Transforms in the Complex Domain (1934)

Introduction Quasi-analytic functions Szasz's theorem Certain integral expansions A class of singular integral equations Entire functions of the exponential type The closure of sets of complex exponential functions Non-harmonic Fourier series and a gap theorem Generalized harmonic analysis in the complex domain The harmonic analysis of random functions Bibliography Index.
Outcomes of Arthroscopic Rotator Cuff Repair in Patients Aged 70 Years or Older (2010)

Level IV, therapeutic case series.
THE VAPOR PRESSURE OF ANHYDROUS OXALIC ACID (1926)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE VAPOR PRESSURE OF ANHYDROUS OXALIC ACIDW. Albert Noyes Jr. and Delbert E. WobbeCite this: J. Am. Chem. Soc. 1926, 48, 7, 1882–1887Publication Date (Print):July 1, 1926Publication History Published online1 May 2002Published inissue 1 July 1926https://pubs.acs.org/doi/10.1021/ja01418a012https://doi.org/10.1021/ja01418a012research-articleACS PublicationsRequest reuse permissionsArticle Views378Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Molecular basis for the interaction between human choline kinase alpha and the SH3 domain of the c-Src tyrosine kinase (2019)

Choline kinase alpha is a 457-residue protein that catalyzes the reaction between ATP and choline to yield ADP and phosphocholine. This metabolic action has been well studied because of choline kinase's link to cancer malignancy and poor patient prognosis. As the myriad of x-ray crystal structures available for this enzyme show, chemotherapeutic drug design has centered on stopping the catalytic activity of choline kinase and reducing the downstream metabolites it produces. Furthermore, these crystal structures only reveal the catalytic domain of the protein, residues 80-457. However, recent studies provide evidence for a non-catalytic protein-binding role for choline kinase alpha. Here, we show that choline kinase alpha interacts with the SH3 domain of c-Src. Co-precipitation assays, surface plasmon resonance, and crystallographic analysis of a 1.5 Å structure demonstrate that this interaction is specific and is mediated by the poly-proline region found N-terminal to the catalytic domain of choline kinase. Taken together, these data offer strong evidence that choline kinase alpha has a heretofore underappreciated role in protein-protein interactions, which offers an exciting new way to approach drug development against this cancer-enhancing protein.