gebhart/patrepeval huggingface dataset:
Affinity Capture of Biotinylated Proteins at Acidic Conditions to Facilitate Hydrogen/Deuterium Exchange Mass Spectrometry Analysis of Multimeric Protein Complexes (2013)

Characterization of conformational and dynamic changes associated with protein interactions can be done by hydrogen/deuterium exchange mass spectrometry (HDX-MS) by comparing the deuterium uptake in the bound and unbound state of the proteins. Investigation of local hydrogen/deuterium exchange in heteromultimeric protein complexes poses a challenge for the method due to the increased complexity of the mixture of peptides originating from all interaction partners in the complex. Previously, interference of peptides from one interaction partner has been removed by immobilizing the intact protein on beads prior to the HDX-MS experiment. However, when studying protein complexes of more than two proteins, immobilization can possibly introduce steric limitations to the interactions. Here, we present a method based on the high affinity biotin-streptavidin interaction that allows selective capture of biotinylated proteins even under the extreme conditions for hydrogen/deuterium exchange quenching i.e. pH 2.5 and 0 °C. This biotin-streptavidin capture strategy allows hydrogen/deuterium exchange to occur in proteins in solution and enables characterization of specific proteins in heteromultimeric protein complexes without interference of peptides originating from other interaction partners in the complex. The biotin-streptavidin strategy has been successfully implemented in a model system with two recombinant monoclonal antibodies that target nonoverlapping epitopes on the human epidermal growth factor receptor (EGFR). We present a workflow for biotinylation and characterization of recombinant antibodies and demonstrate affinity capture of biotinylated antibodies under hydrogen/deuterium exchange quench conditions by the biotin-streptavidin strategy.
The nature of interferon-α resistance in hepatitis C virus infection (2003)

In spite of active research, the role and the mechanisms of IFN resistance in HCV persistence and IFN treatment failure remain partly unknown. A better understanding is needed in order to further improve IFN treatment strategies.
Cerenkov-Specific Contrast Agents for Detection of pH In Vivo (2015)

We have created contrast agents that selectively quench photons emitted during Cerenkov radiation within a given bandwidth. In the presence of a functional dye, such as a pH indicator, this selective quenching allows for a functional determination of pH in vitro and in vivo. This method can be used to obtain functional information from radiolabeled probes using multimodal imaging. This approach allows for the imaging of nonfluorescent chromophores and is generalizable to any functional dye that absorbs at suitable wavelengths.
Gay community links and safety. (1995)

Links to the gay community may strengthen the resolve of those with a strong orientation towards safe sex, but weaken it for those without this resolve. Four Australian studies found statistically significant correlations between unprotected anal intercourse and links to the gay community. The samples from these studies consisted of men who had engaged in widespread unprotected anal intercourse, but who were in no way isolated from the gay community. These results may differ from those obtained from other studies that have sampled gay men without restriction, presumably including many men who found it easier to stick to safe sex practices. These findings suggest that contact with the gay community probably reinforces safe sex behavior among gay men with a safe sex orientation, but may have the opposite effect on those who have difficulty maintaining safe sex. Regardless, contact with the community acts as a multiplier, heightening the effects of existing tendencies. Over-optimism about the effect of community links has been fueled by the inaccurate assumption that men who are HIV-infected will feel a strong sense of responsibility towards their partners. The strongly individualist culture of the 80's and 90's, misguided assumptions on the part of some men participating in unprotected sex, and the strong desire among some men for unprotected anal intercourse contradict the assumption. The author concludes that how gay men relate to one another may often be part of the problem, rather than part of the solution.
Electro-optic KTN deflector stabilized with 405-nm light irradiation for wavelength-swept light source (2017)

We have developed a highly stable electro-optic KTa1-xNbxO3 (KTN) deflector by enhancing electron transportation through KTN crystal. The amount of current is increased with 405-nm light irradiation to rapidly generate a stable refractive-index change, which induces deflection. The deflection angle is set at 160 mrad within tens of seconds and is kept at that angle for 3,000 hours. The developed deflector has been applied to a wavelength-swept light source to measure the thickness of Si wafers with a 3.6-mm optical length. The precision of 0.1-μm has been continuously achieved corresponding to the stability of the KTN deflector.
Supraciliary microstenting: advancing glaucoma treatment in the age of MIGS (2015)

In this article, Dr Ianchulev discusses MIGS supraciliary microstenting, a compelling new approach for outflow enhancement that results in sustained reduction of IOP and anti-hypertensive topical medication use in OAG patients.
Influence of nifedipine on left ventricular dysfunction at rest and during exercise* (1982)

Journal Article Influence of nifedipine on left ventricular dysfunction at rest and during exercise Get access P. HANRATH, P. HANRATH Department of Cardiology, University Hospital HamburgWest Germany Requests for reprints to: Peter Hanrath, M.D., Department of Cardiology, University Hospital Hamburg, Martinistraβe 52, 2000 Hamburg - 20, West Germany. Search for other works by this author on: Oxford Academic PubMed Google Scholar P. KREMER, P. KREMER Department of Cardiology, University Hospital HamburgWest Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar W. BLEIFELD W. BLEIFELD Department of Cardiology, University Hospital HamburgWest Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar European Heart Journal, Volume 3, Issue 4, August 1982, Pages 325–330, https://doi.org/10.1093/oxfordjournals.eurheartj.a061314 Published: 01 August 1982 Article history Received: 30 July 1981 Revision received: 18 November 1981 Published: 01 August 1982
Bronchiolitis in allogeneic bone marrow transplant recipients (1986)

Journal Article Bronchiolitis in allogeneic bone marrow transplant recipients Get access R.H. Hyland, R.H. Hyland Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Correspondence: Dr. R.H. Hyland, Wellesley Hospital, 160 Wellesley Street East, Toronto, Ontario, Canada M4Y U3 Search for other works by this author on: Oxford Academic Google Scholar C.K. Chan, C.K. Chan Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar M.A. Hutcheon, M.A. Hutcheon Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar H.A. Messner, H.A. Messner Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar M. Minden, M. Minden Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar J. Curtis, J. Curtis Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar S.J. Urbanski, S.J. Urbanski Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar J.P. Soulier, J.P. Soulier Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar M. Alexander, M. Alexander Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar M.S. Rodrigues, M.S. Rodrigues Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar ... Show more G. Fyles G. Fyles Departments of Medicine & Pathology, Princess Margaret & Wellesley Hospitals, University of Toronto, Ontario, Canada Search for other works by this author on: Oxford Academic Google Scholar The International Journal Of Cell Cloning, Volume 4, Issue S1, 1986, Pages 203–205, https://doi.org/10.1002/stem.5530040724 Published: 01 January 1996
Analysis of abnormal expression of G‐CSF gene in a novel tumor cell line (KHC 287) elaborating G‐CSF, IL‐1 and IL‐6 with co‐amplification of c‐myc and c‐ki‐ras (1991)

We established a human carcinoma cell line (KHC 287) from a patient with large-cell-typing lung carcinoma associated with marked leukocytosis. The culture supernatant of KHC 287 cells promoted granulocytic colony formation of human bone-marrow cells in semi-solid culture. Colony formation was almost completely suppressed by treatment of the supernatant with a monoclonal anti-granulocyte colony-stimulating factor (G-CSF) antibody. Not only G-CSF but also interleukin-1 alpha (IL-I alpha), IL-I beta and IL-6 were detected in the culture supernatant by an ELISA method. Northern blot analysis of KHC 287 cells revealed distinct expression of these cytokine genes. Southern blot hybridization of KHC 287 DNA showed 20- and 40-fold co-amplification of c-myc and c-ki-ras, respectively. The chloramphenicol acetyl transferase (CAT) activity was distinctly enhanced in the KHC 287 cells which were transfected with the 360 bp upstream region of G-CSF gene inserted into pSV00CAT, but not in non-G-CSF-producing tumor cell lines. These results suggest that overproduction of the transactivating factor(s) which binds to the 360 bp of the G-CSF upstream region is responsible for the abnormal expression of G-CSF gene in KHC 287 cell line.
Synthesis of Gibbilimbols A-D, Cytotoxic and Antibacterial Alkenylphenols Isolated from Piper gibbilimbum (2001)

Gibbilimbols A [(E)-4-(4-decenyl)phenol, 1], B [(E)-4-(3-decenyl)phenol, 2], C [(E)-4-(4-octenyl)phenol, 3] and D [(E)-4-(3-octenyl)phenol, 4] were synthesized by coupling the phenolic parts with the alkyne parts and then reducing the triple bond of the resulting alkynylphenols. These alkenylphenols (1-4) are the cytotoxic and antibacterial constituents of the leaves of a medicinal plant (Piper gibbilimbum) that is used as a traditional medicine in Papua New Guinea.
Editorial: Structure, Function, and Evolution of E3 Ligases and Targets (2021)

EDITORIAL article Front. Plant Sci., 11 October 2021 | https://doi.org/10.3389/fpls.2021.767281
Response-aware Retrieving Candidate Pair for Dialogue Generation (2023)

검색 기반 대화 생성 모델은 대화 데이터베이스에서 가장 관련이 높은 후보 대화 쌍 검색을 목표로 한다. 이전 연구들은 사용자의 질의와 후보 대화 쌍에서의 후보 질의 혹은 후보 답변 중 하나와의 유사도 검사를 진행하였다. 하지만 이런 검색 방법은 사용자 질의 정보만을 사용하기에 검색 성능의 저하를 이끌 수 있다. 그러므로 본 논문에서는 사용자의 질의와 실제 답변을 사용하여 후보 대화 쌍을 검색하는 새로운 방법을 제안한다. 제안하는 검색 방법은 추론 단계에서 실제 답변을 사용할 수 없다는 문제점을 갖고 있기에 이 문제를 해결하기 위해 본 논문에서는 교사-학생 지식 증류 프레임워크를 적용하여 검색모델을 학습한다. 제안 방법은 4개의 대화 생성 데이터셋에 대해 실험적으로 평가되어 우수성을 증명한다.
A new multimodal interactive way of subjective scoring of 3D video quality of experience (2014)

People that watch today's 3D visual programs, such as 3D cinema, 3D TV and 3D games, experience wide and dynamically varying ranges of 3D visual immersion and 3D quality of experience (QoE). It is necessary to be able to deploy reliable methodologies that measure each viewers subjective experience. We propose a new methodology that we call Multimodal Interactive Continuous Scoring of Quality (MICSQ). MICSQ is composed of a device interaction process between the 3D display and a separate device (PC, tablet, etc.) used as an assessment tool, and a human interaction process between the subject(s) and the device. The scoring process is multimodal, using aural and tactile cues to help engage and focus the subject(s) on their tasks. Moreover, the wireless device interaction process makes it possible for multiple subjects to assess 3D QoE simultaneously in a large space such as a movie theater, and at di®erent visual angles and distances.
Enhancement of multiple hypothesis tracking algorithm with C4.5 algorithm (2012)

In this paper, the improvement provided by the utilization of C4.5 classification algorithm to the hypothesis generation process of the k-best Multi-Hypothesis Tracker (MHT) is investigated. It has been shown through simulations that the pre-filtering the measurements by the C4.5 algorithm, especially in high clutter scenarios, reduces the number of generated hypothesis by the MHT significantly.
Distinct T and NK cell populations may serve as immune correlates of protection against symptomatic pandemic influenza A(H1N1) virus infection during pregnancy (2017)

Maternal influenza infection during pregnancy is associated with increased risk of morbidity and mortality. However, the link between the anti-influenza immune responses and health-related risks during infection is not well understood. We have analyzed memory T and NK cell mediated immunity (CMI) responses in pandemic influenza A(H1N1)pdm09 (pdm09) virus infected non-vaccinated pregnant women participating in the Norwegian Influenza Pregnancy Cohort (NorFlu). The cohort includes information on immunization, self-reported health and disease status, and biological samples (plasma and PBMC). Infected cases (N = 75) were defined by having a serum hemagglutination inhibition (HI) titer > = 20 to influenza pdm09 virus at the time of delivery, while controls (N = 75) were randomly selected among non-infected pregnant women (HI titer <10). In ELISpot assays cases had higher frequencies of IFNγ+ CD8+ T cells responding to pdm09 virus or conserved CD8 T cell-restricted influenza A virus epitopes, compared to controls. Within this T cell population, frequencies of CD95+ late effector (CD45RA+CCR7-) and naive (CD45RA+CCR7+) CD8+ memory T cells correlated inversely with self-reported influenza illness (ILI) symptoms. ILI symptoms in infected women were also associated with lower numbers of poly-functional (IFNγ+TNFα+, IL2+IFNγ+, IL2+IFNγ+TNFα+) CD4+ T cells and increased frequencies of IFNγ+CD3-CD7+ NK cells compared to asymptomatic cases, or controls, after stimulation with the pdm09 virus. Taken together, virus specific and functionally distinct T and NK cell populations may serve as cellular immune correlates of clinical outcomes of pandemic influenza disease in pregnant women. Our results may provide information important for future universal influenza vaccine design.
El alumno de bellas artes ante un nuevo paradigma (2011)

Este nuevo paradigma que nos enfrenta a un modelo cuantitativo y cualitativo de la ensenanza-aprendizaje, perturba y enmarana los modos sobre metodos anteriores a la reforma de la ensenanza superior. El papel del alumno, ahora, como actor responsable, en gran medida de su aprendizaje, se concreta y define en el credito ECTS, otorgandole este nuevo rol. En las Facultades de Bellas Artes, la docencia es eminentemente practica. La teoria, va implicita junto al alumno en su puesto de trabajo, mediante un dialogo razonado, he intentando tutelarle al mismo tiempo, para que pilote su propio aprendizaje. El hecho de que el tiempo se limite considerablemente, causa verdaderos estragos en estas ensenanzas
The relationship between CR3 deficiency and neutrophil actin assembly (1989)

Polymorphonuclear leukocytes (PMN) with a deficiency of the complement receptor type 3 (CR3) membrane glycoprotein family have impairments in the ability to adhere to surfaces as well as chemotactic and phagocytic defects, processes that require a functional contractile apparatus. PMN from the patient with neutrophil actin dysfunction (NAD) displayed similar functional characteristics to those with CR3 deficiency suggesting the two disorders may be the same disease. In order to evaluate the relationship between CR3 deficiency and actin assembly, actin filament assembly was measured in PMN from six previously reported homozygotes (two severe and four moderate CR3-deficient patients) as well as five heterozygotes for CR3 deficiency. PMN from all patients had normal unstimulated concentrations of F-actin and after exposure to the chemotactic peptide FMLP (5 x 10(-7) mol/L for 5 to 40 seconds at 25 degrees C) assembled actin normally. Pretreatment of normal PMN with concentrations of monoclonal anti-alpha CR3 antibody, capable of blocking PMN adherence, also failed to impair FMLP- induced actin filament assembly. CR3 glycoprotein expression was measured in PMNs from the mother, father, and older sister of the NAD patient (N Engl J Med 291:1093, 1974). Actin filament assembly was recently shown to be defective in PMNs from all three family members. The total concentrations of the alpha and beta CR3 subunits were below normal in PMN detergent extracts from the mother (25% of simultaneous controls) and older sister (56% of control). PMN surface expression of these two subunits was also found to be depressed (mother, 50%; older sister, 63% of control). These findings suggest these two NAD family members are heterozygote carriers for CR3 deficiency as well as NAD. Simultaneous studies of the father, however, demonstrated normal total concentrations of both the alpha and beta CR3 subunits (126% of controls) as well as normal surface expression of both subunits after phorbol myristate acetate stimulation and incubation at 37 degrees C (mean, 112% of controls) but slightly lower than normal levels after FMLP stimulation (mean, 83%). These findings indicate that CR3 deficiency generally is not associated with defective actin filament assembly and support the conclusion that NAD represents a unique kindred in which PMN actin function differs from previously reported genotypes of CR3 deficiency.
A PROGRAM OF RESEARCH IN DISTRACTION OSTEOGENESIS (2003)

Distraction osteogenesis (DO) is a technique for skeletal lengthening that exploits the body’s innate capacity for bone formation in response to tension forces on fracture callus. DO can also be described as a controlled series of microfractures
Scalable nanomanufacturing of chalcogenide inks: a case study on thermoelectric V–VI nanoplates (2021)

We investigate scalable factors for the synthesis of thermoelectric V–VI chalcogenide nanoplates and systematically study their colloidal behavior and chemical stability in various ink formulations for solution-based additive manufacturing.
Quantitative Annular Dark-Field Imaging at Atomic Resolution (2016)

Quantitative annular dark-filed (ADF) imaging in scanning transmission electron microscopy (STEM) enables us to identify even the type and number of atoms of local crystal structures. A quantification procedure of ADF images was proposed by LeBeau and Stemmer, in which the signal at each pixel is placed on an absolute scale by normalizing the current reaching an ADF detector by the incident probe current The procedure made possible a direct comparison between experimental and simulated ADF images without any arbitrary scaling parameters. We evaluated a nonlinear response of an ADF signal detection system, which was not previously analyzed, and established a quantification procedure applicable to wider experimental conditions Using our procedure, we acquired quantitative ADF images of a few nanomaterials and compared with simulated images to investigate how accurately the scattering intensities match between experiments and simulations.
IGF-IR: Potential Role in Antitumor Agents (2006)

As insulin-like growth factor (IGF) signaling has been recognized to play an important role in human cancer, the IGF-I receptor (IGF-IR) is currently the focus of intensive research aimed at developing novel antitumor agents. The IGF system is frequently deregulated in cancer cells by the establishment of autocrine loops involving IGF-I or -II and/or IGF-IR over-expression. Moreover, epidemiological studies have suggested a link between elevated IGF levels and the development of major human malignancies, such as breast, colon, lung and prostate cancer. Experimental therapies aimed at inhibiting IGF signaling in human tumors involve various approaches, including neutralizing antibodies and pharmacological inhibitors of IGF-IR kinase activity. Although there are numerous reports describing the antitumor activity of such agents against human cancer cell lines propagated in vitro or in experimental animals, it remains unclear how soon the existing drugs will have a demonstrable effect in patients. In this review, we will discuss the evidence implicating the IGF signaling system in the pathology of human cancer and the various strategies that have so far been developed to target the IGF-IR.
Total synthesis of (+)-macbecin I (1990)

The first total enantiospecific synthesis of (+)-macbecin I has been performed in a convergent manner by coupling the epoxide (3) with a higher order cyanocuprate derived from the vinyl iodide (46). The required absolute stereochemistries at C(20)–C(21) and C(12)–C(13) were accessible by enantioselective aldol condensations while that at C(16)–C(17) was achieved by Sharpless epoxidation of a secondary (E)-allylic alcohol (39), efficiently prepared by reaction of the aldehyde (37) with CrCl2-CH3CHI2. The remaining stereocentre at C-18 was introduced by an asymmetric hydroxylation of an enolate. Macrocyclization of the amino acid (59) to give the lactam (60) was successfully achieved by its reaction with either 2-mesitylenesulphonyl chloride or bis(2-oxo-3-oxazolidinyl)phosphinic chloride. Incorporation of the carbamate functionality was achieved by reaction of the parent hydroxy derivative with sodium cyanate and trifluoroacetic acid. The final oxidation to the quinone was accomplished with cerium(IV) ammonium nitrate.
Reversal of Adriamycin-impaired wound healing by transforming growth factor-beta. (1989)

Impaired wound healing remains an important clinical problem. Treatment with systemic Adriamycin (doxorubicin) is known to impair wound healing in patients, and it has been used to produce animal models of impaired healing. The results of previous studies have shown that local treatment of incisions in normal rats with transforming growth factor beta (TGF-beta) or epidermal growth factor (EGF) stimulated early increases in tensile strength of surgical incisions in normal rats. We investigated the effects of locally applied, biosynthetic TGF-beta or EGF on the tensile strength of standardized incisions in rats treated with Adriamycin. Systemic Adriamycin treatment (8 milligrams per kilogram) produced significant decreases in wound tear strength (WTS) and wound tear energy (WTE) when compared with that of normal rats at seven and ten days (p less than 0.01). A single dose of TGF-beta (2 micrograms) in a collagen vehicle stimulated a reversal of this wound healing impairment at ten days (p less than 0.05), returning the WTS and WTE to near normal levels. A single dose of EGF (50 micrograms) in hyaluronic acid failed to increase tensile strength, probably because of formulation of EGF in a vehicle that does not prolong its release in incisions. These results suggest that exogenous growth factors may be clinically useful in stimulating healing in incisions in healing impaired conditions.