gebhart/patrepeval huggingface dataset:
Interrelationship between the Microbial Communities of the Root Canals and Periodontal Pockets in Combined Endodontic-Periodontal Diseases (2021)

Periodontal and Endodontic diseases are biofilm-related diseases. The presence of microorganisms in root canals (RCs) and the complex microbiota of periodontal pockets (PPs) contribute to the development of endodontic-periodontal diseases. This study performed a systemic analysis using state-of-the-art sequence data to assess the microbial composition of infected RCs and PPs to further assess the microbiota and verify the possibility of cross-infection between these sites. The microbiomes of these combined diseases were examined with a focus on the V3-V4 hypervariable region of the 16S rRNA gene. The number of species in PP was higher than in RC, and there was a predominance of obligate anaerobes and gram-negative bacteria. In the RCs, the genera <i>Enterococcus</i>, <i>Parvimonas</i>, <i>Stomatobaculum</i> predominated, in contrast, the PPs revealed a predominance of <i>Enterococcus</i>, <i>Parvimonas</i>, <i>Stomatobaculum</i>, <i>Peptostreptococcus</i> and <i>Mogibacterium.</i> The RC and PP microbiome was not similar with regards to the sharing of OTUs for phyla and genera (8 and 67, respectively). The evaluation of molecular markers revealed a large number of markers for resistance to antibiotics of the carbapenem and beta-lactam type (broad spectrum). Another relevant finding of this study was the markers related to systemic diseases related to cardiac muscle and rheumatology, among others. In conclusion, the RC microbiota was less complex and diverse than PP. Interactions between microbial communities were present. The shared genus can signal communication between the endodontic and periodontal microbiomes.
Quantitative Immunohistochemical Determination of Cathepsin D Levels in Prostatic Carcinoma Biopsies:Correlation with Tumor Grade, Stage, PSA Level, and DNA Ploidy Status (1995)

National screening programs resulting in an increased detection rate of prostatic adenocarcinoma have prompted the search for new methods of predicting disease outcome that can be applied to the initial narrow bore needle biopsy specimens. Cathepsin D, a lysosomal aspartyl protease and autocrine mitogen, has been studied in a wide variety of human neoplasms as an invasion and metastasis marker. Prostatic carcinoma needle biopsy tumor cell cathepsin D content was measured in 61 men using a semiquantitative image analysis assisted immunohistochemical procedure. Results were compared with preoperative serum prostatic specific antigen levels, tumor grade, DNA ploidy status, pathologic stage after radical prostatectomy and disease recurrence during a median 2.6 year follow-up. Biopsy cathepsin D levels significantly correlated with tumor grade (P = .022) and DNA ploidy status (P = .028) by logistic regression analysis. Post-prostatectomy pathologic stage and disease recurrence did not correlate with tumor cathepsin D levels. Final prostatectomy grade and DNA ploidy status independently predicted metastasis and post-operative disease recurrence (P < .001). Although this study did not find independent prognostic status for cathepsin D in prostate cancer, the correlation with tumor grade and DNA ploidy status is noteworthy and the inter-relationship of outcome variables may prove of interest and warrant further evaluation of this potential predictor or CO-predictor of disease outcome.
Asymmetric Addition of Arylboroxines to Aryl Ketones by Rh(I) Catalysis (2016)

Key words rhodium catalysis - asymmetric intermolecular addition - tertiary alcohols - arylboroxines
CuBr Crystal Growth in Ethylene Glycol Solvent by the Temperature-Difference Method (2004)

A novel temperature-difference method was applied to produce CuBr crystals by using ethylene glycol as solvent and KBr as mineralizer; the temperature of the growth zone and the dissolution zone are 150 °C and 110 °C, respectively. The process was performed at ambient pressure. Clear, triangular, millimeter-scaled CuBr single crystals were obtained. The crystals were characterized by powder X-ray diffraction and selected area electron diffraction.
A Case of Empty Nose Syndrome Developed After Septal Spur Removal (2022)

Empty nose syndrome (ENS) is a rare complication of turbinate surgery, in which patients suffer from symptoms such as paradoxical nasal obstruction, nasal dryness, facial pain and feeling excessive nasal openness. Its occurrence is generally attributed to excessive turbinate reductive procedures. However, theoretically, any procedure which can cause increased nasal airflow and decreased nasal resistance may have a risk of causing ENS. We recently experienced a case of ENS developed after septal spur removal without turbinate reduction, which has not previously been reported in the literature. Here we present the case history of this unusual occurrence.
Model-Based Stator Fault Detection in Induction Motors (2008)

This paper addresses the subject of fault detection in the electrical parts of induction motors. In particular inter-turn short circuit faults are considered. The detection scheme is based on a motor-model, derived in the paper, which does not include the motor speed. This makes the detection scheme robust to any speed and torque changes on the motor shaft. The efficiency of the proposed detection scheme is demonstrated by simulations and by tests performed on a test setup with a customized designed induction motor.
Dissociation of Duplexes Formed by Hybridization of DNA with Gel-Immobilized Oligonucleotides (1994)

The method of DNA sequencing by hybridization with oligonucleotides matrix (SHOM) developed in this laboratory (1.2) uses the matrix of oligonucleotides immobilized within polyacrylamide gel. The particular feature of this matrix is that the apparent thermostability of the duplexes depends on the concentration of gel-immobilized oligonucleotides. This dependence is specific for oligonucleotides immobilized in the gel volume (3-D-immobilization) rather than on a flat surface of a filter or glass (2-D-immobilization). The theory has been developed that provides a quantitative description of temperature-dependent duplex dissociation within gel. The theory takes into account that the diffusion of dissociated DNA out of the gel is retarded by multiple acts of association-dissociation of DNA with immobilized oligonucleotides. It allows to calculate the apparent dissociation temperature of duplexes and describes quantitatively its growth upon increase in the enthalpy of duplex dissociation, concentration of immobilized oligonucleotides, gel thickness and decrease of dissociation entropy and washing time. Concentration of gel-immobilized oligonucleotides can be calculated for a normalized matrix in which GC-rich and AT-rich duplexes exhibit the same apparent thermostabilities and are washed off at the same temperature. This simplifies identification of perfect duplexes formed on the matrix which can be carried out for all duplexes at the same temperature. The gel-immobilized oligonucleotide matrix provides also a higher capacity for immobilization and therefore a higher sensitivity of measurements, resulting in a higher discrimination power for identification of perfect duplexes as compared with matrixes of oligonucleotides immobilized on a surface.
An integrated design flow for a via-configurable gate array (2005)

In This work we present a complete physical design flow for a via-configurable gate array (VCGA). The VCGA is an array of prefabricated logic blocks and fixed metal masks. The block consists of via-configurable functional cells and a via-decomposable flip-flop. An M1-M2 via mask is used to define the block's functionality. Interconnects are customized using via masks. We developed a physical design flow for VCGA, which integrates a set of effective techniques. Here, we highlight the packing, cell-binding, and detailed-routing problems. We use our design flow to compare the VCGA-based and standard-cell/FPGA-based designs. Experimental results show the efficiency of our flow.
Identification of separate mRNAs coding for the alpha and beta subunits of thyrotropin. (1979)

A 9S mRNA, purified from mouse thyrotropic pituitary tumors by sucrose density gradient centrifugation of poly(A)-enriched mRNA, directed the synthesis of only alpha and beta subunits of thyrotropin in the reticulocyte lysate translation system. Analysis of radioiodinated 9S mRNA, repurified by oligo(dT)-cellulose chromatography and sucrose gradient centrifugation, yielded two species of RNA on urea/polyacrylamide gel electrophoresis. The major RNA species contained 620 nucleotides, and the minor RNA species contained 560 nucleotides. Unlabeled 9S mRNA was further purified by urea/polyacrylamide gel electrophoresis; the mRNAs were separately eluted from slices of the gel containing material migrating with an apparent length of 620 and 560 nucleotides. Translation of these mRNAs in the reticulocyte lysate showed that the longer mRNA coded for the alpha subunit and the shorter mRNA coded for the beta subunit of mouse thyrotropin. Because more alpha than beta subunit of thyrotropin was consistently synthesized, unbalanced amounts of thyrotropin subunits appear to be synthesized by translation of unbalanced amounts of individual mRNAs. We have demonstrated that the synthesis of thyrotropin is directed by two separate mRNA molecules, each coding for a different subunit of the hormone.
Videokeratoscope for accurate and detailed measurement of the cornea surface (2005)

A videokeratoscope based on the imaging of a gridlike pattern is introduced. Unlike conventional videokeratoscopes that rely on Placido disks, the new measurement principle allows an exact reconstruction of the surface and the display of fine details. The experimental instrument was tested on precision glass spheres; the maximum error of the height data was less than 3 microm. The sensitivity and the potential to resolve fine details were demonstrated with irregular surfaces of specially prepared contact lenses. Structures with height deviations of 0.1 microm are well identifiable. The eyes of 18 individuals were investigated, and fine structures were found on these cornea surfaces. Conventional videokeratoscopes do not resolve such detail.
T0501-5-1 Skin moisture device based on micro heat transfer (2010)

This paper reports the skin moisture device based on micro heat transfer. Moisture density of a target was measured by heat transfer across the interface. Our device consists of resistor temperature detectors (RTD) and a pressure sensor. The RTDs were also used as a heat source. Our device detected difference in moisture density of a contact object by measuring step responses of RTDs in 3 seconds. Experimental data shown for the moisture density in the human skin was actually detectable with our device.
2050 nm Pulse Picker Based Femtosecond Chirped Pulse Fiber Amplifier System at 362 nJ and 800 kHz (2015)

The field of ultrashort pulsed laser sources in the 2 μm wavelength region is rapidly evolving, especially as frontend lasers for pumping optic parametric devices [1,2] or solid state Ho-based amplifiers [3]. Furthermore, pulsed laser sources are highly suitable for electron acceleration applications due to the wavelength scaling of the dephasing length for nonrelativistic and relativistic electrons [4]. High pulse energy chirped pulse amplifier (CPA) systems incorporating a single chirped bragg grating (CVBG) and a pulse picking device have been demonstrated, but were yet limited to the Erbium-wavelength range [5]. Common environmentally stable all-polarization maintaining (PM) femtosecond lasers at 2 μm are bound to quite high repetition rates in the MHz regime, yet limiting the contrast between pulse energy and average output power.
Correlations between different measures of clinic, home, and ambulatory blood pressure in hypertensive patients (2011)

Clinic BP measured with the automated monitor could be used as an alternative for the evaluation of BP in the office. Under rigorously standardized conditions, clinic and home BP could be used as an alternative to awake ABP.
Real-Time Statistical Process Control (1994)

This book demonstrates how to use real-time statistical process control (SPC) in the process industries in order to maintain and improve quality and increase productivity. It presents appropriate SPC tools and shows how to use them for actual operation of process plants. It also shows how real-time SPC charts use electronically collected and distributed real-time database for real-time monitoring, and explains how to take into account processes dynamics in forming subgroups.
Personality traits in mood disorders: Association with polymorphisms of the dopamine D 3 receptor gene? (1998)

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
[Intracardial shock for the conversion of chronic atrial fibrillation]. (1990)

Internal cardioversion was attempted in three patients with chronic atrial fibrillation, refractory to conventional external direct current shocks. Endocardiac shocks were delivered between the proximal electrode on a multipolar USCI pacing catheter and a back paddle. This is positioned with the tip proximal of the bundle of His, and the proximal ring electrode floating in the right atrium. Two patients were converted with 200 Joules, one needed 400 Joules. Two of our three patients were chronically maintained in sinus rhythm after the initial conversion, one had recurrence within 24 hours.
Tuning the Thickness of a Biomembrane by Stapling Diamidophospholipids with Bolalipids (2020)

In a biological membrane, proteins require specific lipids of distinctive length and chain saturation surrounding them. The active tuning of the membrane thickness therefore opens new possibilities in the study and manipulation of membrane proteins. Here, we introduce the concept of stapling phospholipids to different degrees of interdigitation depth by mixing 1,3-diamidophospholipids with single-chain bolalipids. The mixed membranes were studied by calorimetric assays, electron microscopy, X-ray, and infrared measurements to provide a complete biophysical characterization of membrane stapling. The matching between the diamidophospholipids and the bolalipids can be so strong as to completely induce a new phase that is more stable than the gel phase of the individual components.
Synthesis of Diselenadiazafulvalenes and Influence of Steric Strain on Their Anodic Behavior. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
The Challenges of Analysing Highly Diverse Picobirnavirus Sequence Data (2018)

The reliable identification and classification of infectious diseases is critical for understanding their biology and controlling their impact. Recent advances in sequencing technology have allowed insight into the remarkable diversity of the virosphere, of which a large component remains undiscovered. For these emerging or undescribed viruses, the process of classifying unknown sequences is heavily reliant on existing nucleotide sequence information in public databases. However, due to the enormous diversity of viruses, and past focus on the most prevalent and impactful virus types, databases are often incomplete. Picobirnaviridae is a dsRNA virus family with broad host and geographic range, but with relatively little sequence information in public databases. The family contains one genus, <i>Picobirnavirus</i>, which may be associated with gastric illness in humans and animals. Little further information is available due in part to difficulties in identification. Here, we investigate diversity both within the genus <i>Picobirnavirus</i> and among other dsRNA virus types using a combined phylogenetic and functional (protein structure homology-modelling) approach. Our results show that diversity within picobirnavirus exceeds that seen between many other dsRNA genera. Furthermore, we find that commonly used practices employed to classify picobirnavirus, such as analysis of short fragments and trimming of sequences, can influence phylogenetic conclusions. The degree of phylogenetic and functional divergence among picobirnavirus sequences in our study suggests an enormous undiscovered diversity, which contributes to the undescribed "viral dark matter" component of metagenomic studies.
Bridging of Gold Nanoparticles by Functional Polyphenylene Dendrimers (2009)

We describe a protocol for the in-suspension bridging of gold particles with shape-persistent polyphenylene dendrimers, carrying multiple lipoic acid groups at their periphery. A solvent mixture is identified where both reactants are stable as individuals. Upon mixing, aggregates with stoichiometry-dictated size form rapidly. Initially, the spacing between individual gold colloids is large enough that their absorption spectrum is only slightly different from isolated particles. The aggregates rearrange within hours, leading to strong optical coupling between the gold particles and a corresponding spectral shift.
Critical communications and public safety networks, part 2: technical issues, security, and applications [Guest Editorial] (2016)

As we mentioned in the Guest Editorial of Part 1 of this Feature Topic, which was published in March 2016, due to a high number of high quality submissions, we divided the accepted papers into two parts. While the articles in Part 1 focused on general topics such as overview, spectrum policies, and economics, the articles of Part 2 in this issue of the magazine focus on more technical issues and solutions.
Probabilistic methods for renewable energy sources and associated electrical loads for Southern African distribution systems (2009)

Significant socio-political pressure is being exerted on utilities throughout the world to expand their production and delivery of electrical energy by including renewable energy sources. To conduct meaningful analyses so that decisions may be taken in the procedures of planning, design, economics and technology, appropriate models must be derived for both loads and energy sources. The most difficult and the most important aspect in this type of modeling is the inclusion of uncertainty.
Swelling, reductive stress, and cell death during chemical hypoxia in hepatocytes (1989)

In rat hepatocytes, we examined the relationship between cell volume, bleb formation, and loss of cell viability during chemical hypoxia with KCN plus iodoacetic acid. In hypotonic media (150-200 mosmol/kgH2O), cells swelled to a greater extent during chemical hypoxia than in isotonic media, but rates of cell killing were identical. Sucrose (300 mM) added to isotonic media prevented early cell swelling but actually accelerated cell killing. In contrast, mannitol (300 mM) improved cell survival but did not prevent cell swelling. Bleb formation occurred regardless of buffer tonicity. The antioxidants desferrioxamine and cyanidanol but not superoxide dismutase +/- catalase delayed lethal cell injury. Cell killing was greater during aerobic compared with anaerobic chemical hypoxia. Hydroperoxide formation was measured using a dichlorofluorescin assay and was accelerated during aerobic but not anaerobic chemical hypoxia. The results indicate that cell swelling is not the driving force for bleb formation or lethal cell injury. We conclude that "reductive stress" caused by respiratory inhibition favors formation of toxic oxygen species and may contribute to lethal cell injury during intermittent or incomplete oxygen deprivation.
Onboard Product Generation on Earth Observing One: A Pathfinder for the Proposed Hyspiri Mission Intelligent Payload Module (2013)

The proposed HyspIRI mission is evaluating a X-band Direct Broadcast capability that would enable data to be delivered to ground stations virtually as it is acquired. However the HyspIRI VSWIR and TIR instruments are expected to produce over 800 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">6</sup> bits per second of data while the Direct Broadcast capability is approximately 10 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">6</sup> bits per second for a ~ 80x oversubscription. In order to address this data throughput mismatch a Direct Broadcast concept called the Intelligent Payload Module (IPM) has been developed to determine which data to downlink based on both the type of surface the spacecraft is overlying and onboard processing of the data to detect events. For example, when the spacecraft is overlying polar regions it might downlink a snow/ice product. Additionally the onboard software would search for thermal signatures indicative of a volcanic event or wild fire and downlink summary information (extent, spectra) when detected. Earth Observing One (EO-1) has served as a test bed and pathfinder for this type of onboard product generation. As part of the Autonomous Sciencecraft (ASE), EO-1 implemented in ίight software the ability to analyze and develop products for a limited swath of the Hyperion hyperspectral instrument onboard the spacecraft. In a series of technology demonstrations that became part of the operational EO-1 system over 5000 science products have been generated onboard EO-1 and down linked via engineering S-band contacts, a routine automated process that continues to this day. We describe the onboard products demonstrated in EO-1 operations and show how they have paved the way for the HyspIRI Intelligent Payload Module concept.
Large-Area Niobium Titanium Nitride Superconducting Microstrip Single-Photon Detector Fabricated Using a Photolithography Process (2023)

In this paper, we present a large-area niobium titanium nitride (NbTiN) superconducting microstrip single-photon detector (SMSPD) fabricated using a photolithography process. We fabricated a 1-μm-wide NbTiN-SMSPD covering 420 × 420 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> active area with a filling factor of 20% using an i-line stepper. The detector is cooled down to approximately 2.2 K, and its performance is evaluated at the wavelengths from visible to near-infrared. The detector operates stably without a shunt resistor and shows single-photon sensitivity for photons with a wavelength of 1550 nm. The bias current dependence of the detection count rate is measured at the wavelengths from 406 to 1550 nm. The saturation of the detection count rate with increasing the bias current was successfully observed at wavelengths below 850 nm, suggesting an internal detection efficiency approaching 100%. Furthermore, we evaluated the timing jitter performance using the femtosecond pulse laser with a wavelength of 1550 nm. Consequently, we achieved a system timing jitter of 85 ps for a light spot diameter of 100 μm.
General M13 Phage Display: M13 Phage Display in Identification and Characterization of Protein–Protein Interactions (2009)

In M13 phage display, proteins and peptides are exposed on one of the surface proteins of filamentous phage particles and become accessible to affinity enrichment against a bait of interest. We describe the construction of fragmented whole genome and gene fragment phage display libraries and interaction selection by panning. This strategy allows the identification and characterization of interacting proteins on a genomic scale by screening the fragmented "proteome" against protein baits. Gene fragment libraries allow a more in depth characterization of the protein-protein interaction site by identification of the protein region involved in the interaction.