gebhart/patrepeval huggingface dataset:
Development of a Novel Anti-CD19 Chimeric Antigen Receptor: A Paradigm for an Affordable CAR T Cell Production at Academic Institutions (2018)

Genetically modifying autologous T cells to express an anti-CD19 chimeric antigen receptor (CAR) has shown impressive response rates for the treatment of CD19+ B cell malignancies in several clinical trials (CTs). Making this treatment available to our patients prompted us to develop a novel CART19 based on our own anti-CD19 antibody (A3B1), followed by CD8 hinge and transmembrane region, 4-1BB- and CD3z-signaling domains. We show that A3B1 CAR T cells are highly cytotoxic and specific against CD19+ cells <i>in vitro</i>, inducing secretion of pro-inflammatory cytokines and CAR T cell proliferation. <i>In vivo</i>, A3B1 CAR T cells are able to fully control disease progression in an NOD.Cg-<i>Prkdc</i> <sup><i>scid</i></sup> <i>Il2rd</i> <sup><i>tm1Wjl</i></sup> /SzJ (NSG) xenograph B-ALL mouse model. Based on the pre-clinical data, we conclude that our CART19 is clearly functional against CD19+ cells, to a level similar to other CAR19s currently being used in the clinic. Concurrently, we describe the implementation of our CAR T cell production system, using lentiviral vector and CliniMACS Prodigy, within a medium-sized academic institution. The results of the validation phase show our system is robust and reproducible, while maintaining a low cost that is affordable for academic institutions. Our model can serve as a paradigm for similar institutions, and it may help to make CAR T cell treatment available to all patients.
High‐order volterra series analysis using parallel computing (1997)

This paper addresses the determination of high-order Volterra transfer functions of non-linear multiport networks containing multidimensional non-linear elements. A new method is developed for utilizing parallel computing which is very efficient for approximately 10–20 processors. The utilization of each processor may be as high as 80%–95%. The developed program is very flexible as it is ANSI C++ compatible and may run on both single- and multiprocessor computers. Using about eight processors it is possible to analyse rather complicated non-linear circuits up to ninth order in a few hours. © 1997 by John Wiley & Sons, Ltd.
The MTHFR 677C→T polymorphism and behaviors in children with autism: exploratory genotype–phenotype correlations (2009)

New evidence suggests that autism may be associated with (a) varied behavioral responses to folate therapy and (b) metabolic anomalies, including those in folate metabolism, that contribute to hypomethylation of DNA. We hypothesized that children with autism who are homozygous for the MTHFR 677 T allele (TT) and, to a lesser extent those with the CT variant, would exhibit more behavioral problems and/or more severe problematic behaviors than homozygous wild-type (CC) individuals because of difficulties in effectively converting 5,10-MTHF to 5-MTHF. Data from the Autism Genetic Resource Exchange (AGRE) collection were analyzed for all children who met strict criteria for autism per the Autism Diagnostic Interview-Revised (ADI-R) and the Autism Diagnostic Observation Schedule (ADOS) and who had been genotyped for the 677 C to T MTHFR polymorphism (n=147). Chi-square tests, logistic regression, and one-way ANOVAs were used to determine whether differences existed among MTHFR genotypes for specific behaviors on the ADI-R and indices for level of functioning. Exploratory results indicated four behaviors from the ADI-R that were more common and problematic (95% CI) among those with at least one copy of the T allele as compared to homozygous wild-type individuals: direct gaze, current complex body movements, a history of self-injurious behavior, and current overactivity (ORs=2.72, 2.33, 2.12, 2.47, respectively). No differences existed among genotypes for level of functioning as measured with the Peabody Picture Vocabulary Test-Third Edition, Ravens Colored Progressive Matrices, or the Vineland Adaptive Behavior Scales. Findings call for further investigation of the relationship between folate metabolism and problem behaviors among children with autism.
Role of NADH oxidase in the oxidative inactivation of Streptococcus salivarius fructosyltransferase (1986)

A cell-associated fructosyltransferase produced by Streptococcus salivarius was irreversibly inactivated in a time-dependent manner when resting or permeabilized cell suspensions were incubated with low concentrations (less than 1.0 microM) of copper. In addition to copper, the inactivation was dependent on oxygen and on a fermentable carbon source (endogenous intracellular polysaccharide or glucose). In starved, permeabilized cell suspensions, the fermentable carbon source could be replaced by NADH but not by NADPH or ATP. Of several other S. salivarius enzymes tested, only fructosyltransferase was inactivated under these conditions. The available evidence indicated that NADH oxidase is the enzyme responsible for fructosyltransferase inactivation. Results from oxygen radical scavenger studies implicated one or more species of oxygen radicals and hydrogen peroxide in the inactivation reaction.
Integrated Fault-Tolerant Flight Control System with Actuator Amplitude Saturation (2019)

An integrated control scheme using fault diagnosis and reconfigurable control is investigated in this paper for a commercial aircraft with actuator faults and amplitude saturation. Actuator faults and amplitude saturation are both important factors adversely affecting the stability and performance of flight control systems. The fault diagnosis process is designed based upon a modified multiple-model based unknown input observer and the reconfigurable control is constructed using adaptive control. Both additive and multiplicative faults are considered in the integrated design to deal with the three types of actuator faults. Moreover, different techniques are implemented in the basic and fault-tolerant controller to anti-windup. Finally, a numerical example of the integrated fault-tolerant controller for a Boeing-747 is demonstrated. The stability and performance improvements can be accrued with the presented fault-tolerant control scheme.
Modern lens design : a resource manual (1992)

Lens design techniques automatic lens design aberration presentation and interpretation lens designs, prescriptions, performance, comments and advice singlets, telescope objectives, eyepieces, Cooke triplets, tessars, heliars, split triplets, meniscus types, double Gauss objectives, wide angle objectives, microscoe objectives, laser collimator/disk objectives, telephoto objectives, retrofocus objectives, scanner lenses, infrared systems, afocal systems/Beam expanders, zoom lenses, anamorphic attachments, relay, erector, periscope, endoscope systems.
A New Method for the Determination of Specific13C Enrichment in Phosphorylated [1–13C]glucose Metabolites (1996)

A 31P-NMR method for the determination of 13C enrichment in phosphorylated [1-(13)C]glucose metabolites was developed by taking advantage of the 13C satellites detectable for 31P-NMR signals of metabolites such as UDP-hexoses, UDP-N-acetylhexosamines and other phosphorylated compounds generated during glycolysis and subsequent anabolism. HT-29 cells were incubated in culture medium containing 4.5 g/l [1-(13)C]glucose for 24 h prior to cell extraction, and high-resolution 31P-NMR spectra were acquired from perchloric acid extracts. Since glucose and its phosphorylated products are key metabolites for many different metabolic processes, this method may be very helpful for studying specific metabolic pathways involving phosphorylated glucose metabolites.
Supplementary Figure 2 from Nitric Oxide Inactivates the Retinoblastoma Pathway in Chronic Inflammation (2023)

Supplementary Figure 2 from Nitric Oxide Inactivates the Retinoblastoma Pathway in Chronic Inflammation
Robin hood hashing (1985)

This paper deals with hash tables in which conflicts are resolved by open addressing. The initial contribution is a very simple insertion procedure which (in comparison to the standard approach) has the effect of dramatically reducing the variance of the number of probes required for a search. This leads to a new search procedure which requires only a constant number of probes, on average, even for full tables. Finally, an extension to these methods yields a new, simple way of performing deletions and subsequent insertions. Experimental results strongly indicate little degeneration in search time. In particular deletions and successful searches appear to require constant time (≪ 2.57 probes) and insertions and unsuccessful searches, O(logn).
Intralimb incoordination in patients with ataxia (2003)

Intralimb incoordination needs to be distinguished from interlimb incoordination, which is observed in Parkinson's disease (PD). With this aim, intra and inter-limb coordination impairment in the lower limbs were measured in 13 patients with cerebellar ataxia during pedaling of an ergometer with left and right pedals that can be rotated independently. Intralimb incoordination in patients with cerebellar ataxia was compared with interlimb incoordiation in patients with PD. We concluded that impairment of intralimb coordination in ataxia patients leads to inability to maintain pedaling amplitude and speed, but unlike in PD, right and left coordination was roughly preserved. Our method may be useful for assessing the severity of ataxia and detecting hidden parkinsonism in ataxic patients.
STAIRoute: Early Global Routing using Monotone Staircases for Congestion Reduction (2018)

With aggressively shrinking process nodes, physical design methods face severe challenges due to poor convergence and uncertainty in getting an optimal solution. An early detection of potential failures is thus mandated. This has encouraged to devise a feedback mechanism from a lower abstraction level of the design flow to the higher ones, such as placement driven synthesis, routability (timing) driven placement etc. Motivated by this, we propose an early global routing framework using pattern routing following the floorplanning stage. We assess feasibility of a floorplan topology of a given design by estimating routability, routed wirelength and vias count while addressing the global congestion scenario across the layout. Different capacity profiles for the routing regions, such as uniform or non-uniform different cases of metal pitch variation across the metals layers ensures adaptability to technology scaling. The proposed algorithm STAIRoute takes $O(n^2kt)$ time for a given design with $n$ blocks and $k$ nets having at most $t$ terminals. Experimental results on a set of floorplanning benchmark circuits show $100\%$ routing completion, with no over-congestion in the routing regions reported. The wirelength for the $t$-terminal ($t\geq$ 2) nets is comparable with the Steiner length computed by FLUTE. An estimation on the number of vias for different capacity profiles is also presented, along with congestion and runtime results.
Purification and characterization of a type II DNA topoisomerase from bovine calf thymus. (1985)

We report here the large scale purification of DNA topoisomerase II from calf thymus glands, using the unknotting of naturally knotted P4 phage DNA as an assay for enzymatic activity. Topoisomerase II was purified more than 1300-fold as compared to the whole cell homogenate, with 22% yield. Analysis of the purified enzyme by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed two bands of apparent molecular masses of 125 and 140 kDa. Tryptic maps of the two bands indicated that they derive from the same protein. Using these fragments, specific polyclonal antisera to topoisomerase II were raised in rabbits. Immunoblotting of whole cell lysates from various species indicated that topoisomerase II is well conserved among mammals and has a native subunit molecular mass of 180 kDa. Analytical sedimentation and gel filtration were used to determine a sedimentation coefficient of 9.8 S and a Stokes radius of 68 A. The calculated solution molecular mass of 277 kDa implies a dimer structure in solution. The purified topoisomerase II unknots P4 DNA in an ATP-dependent manner and is highly stimulated in its relaxation activity by ATP. A DNA-stimulated ATPase activity, as has been found with other type II topoisomerases, is associated with the purified enzyme. Approximate kinetic parameters for the ATPase reaction were determined to be: a Vmax of 0.06 nmol of ATP/(micrograms of protein) (min) and Km of 0.2 mM in the absence of DNA, and a Vmax of 0.2 nmol of ATP/(micrograms of protein) (min) and Km of 0.4 mM ATP in the presence of supercoiled plasmid DNA.
Correlación entre la medida del color del fruto y la concentración de sólidos solubles totales en frutilla o fresa (Fragaria ananassa Duch.) (2015)

La cuantificación de la calidad de la frutilla se realiza generalmente mediante la determinación de la cantidad de sólidos solubles totales (SST) utilizando técnicas refractométricas. El objetivo de este trabajo fue correlacionar estas medidas de calidad con los parámetros obtenidos durante la determinación del color de los frutos utilizando dos instrumentos colorimétricos distintos. El estudio se realizó en frutos de tres variedades de frutilla (?Camarosa?, ?Florida Festival? y ?Camino Real?) obtenidos durante la temporada 2013 en la Estación Experimental Agropecuaria Famaillá ? INTA. En cada fruto se midió el contenido de SST y las variables colorimétricas en dos espacios de color diferentes. Se ajustaron distintos modelos para obtener la mejor correlación entre ambas medidas. Los ajustes mostraron que la mejor correlación se obtiene utilizando el espacio de color CIE L*a*b*. Además, el ajuste más significativo se obtuvo apelando a la variable a*, que codifica la cantidad de rojo del fruto. Sin embargo, los errores del ajuste no permiten obtener una predicción precisa de la calidad a partir de la determinación del color. El error se encuentra en el orden de ± 3 °Brix.
Efficacy and safety of neoadjuvant trastuzumab combined with paclitaxel and epirubicin (2007)

Stage II and III HER2-positive breast cancer patients achieved a high rate of PCR with trastuzumab given concurrently with paclitaxel and FEC(75) chemotherapy. No severe cardiac events were observed with the regimen. The data concur with the results of a previously published trial.
Case 4-2021: A 70-Year-Old Woman with Dyspnea on Exertion and Abnormal Findings on Chest Imaging (2021)

A 70-year-old woman was evaluated because of increasing dyspnea on exertion and abnormal findings on chest imaging. Eleven years before this evaluation, mild mosaic attenuation at the lung bases was identified incidentally. Three years later, dyspnea on exertion developed and pulmonary function worsened. Diagnostic tests were performed.
Genome Sequence of Lipopeptide- and Antioxidant-Producing Strain Bacillus velezensis NWUMFkBS10.5 (2019)

Candidate biocontrol agent <i>Bacillus velezensis</i> NWUMFkBS10.5 possesses unique genomic characteristics revealed by antiSMASH analysis and <i>in vitro</i> metabolomic elucidation. Besides its capability to produce antimicrobial lipopeptides, further <i>in silico</i> genome profiling predicted the presence of metabolic pathways for synthesizing antioxidants like lampranthin-2, miraxanthin V, and 2-decarboxybetanidin.
The nervous system (1969)

Introduction. Functions and structure of the nervous system of vertebrates examining the world light receptors - looking sound receptors - listening olfactory receptors - smelling gustatory receptors - tasting cutaneous receptors - touching and feeling receptors for the inside world nerves and nerve fibres communication within the nervous system standing and moving awake and exploring - relaxed and sleeping needs, desires and emotions pain the centre of the brain - the hypothalamus broadcasting information - hormones general plan of the human brain exploring man's living brain sensation acquires a meaning speech and other symbols learning remembering personality and the brain. Glossary. Index.
Dechloromonas hortensis sp. nov. and strain ASK-1, two novel (per)chlorate-reducing bacteria, and taxonomic description of strain GR-1 (2005)

Recent studies on the occurrence of (per)chlorate-reducing bacteria have resulted in the characterization of strains capable of dissimilatory (per)chlorate reduction. Phylogenetic analysis has shown that these bacteria are members of the Proteobacteria. Strains have been isolated from polluted and pristine sites, but only strains from polluted sites have been characterized in detail and deposited in culture collections. Herein we describe the isolation and characterization of perchlorate-reducing bacterium strain MA-1(T) and chlorate-reducing bacterium strain ASK-1, respectively isolated from a pristine and a chlorate-polluted site. Both isolates are members of the Proteobacteria. The 16S rRNA gene sequence similarity of MA-1(T) to Dechloromonas agitata DSM 13637(T) is 97.6%, but the relatedness in DNA-DNA reassociation is only 37%. Therefore, we propose to classify strain MA-1(T) (=DSM 15637(T)=ATCC BAA-776(T)) as the type strain of a novel species, Dechloromonas hortensis sp. nov. Strain ASK-1 and a previously described strain GR-1 show 100 and 99% 16S rRNA gene sequence similarity to Pseudomonas chloritidismutans DSM 13592(T) and Dechlorosoma suillum DSM 13638(T), respectively. DNA-DNA hybridization studies indicated that strains ASK-1 and GR-1 are related at the species level to P. chloritidismutans DSM 13592(T) (79%) and Dechlorosoma suillum DSM 13638(T) (85%), respectively. As suggested previously, Dechlorosoma suillum appears to be a later heterotypic synonym of Azospira oryzae. Although strain ASK-1 is identified as P. chloritidismutans, its morphology and growth requirements are different from those of the type strain.
Effect of post treatments on the structure and thermal stability of titanate nanotubes (2006)

TiO2 sol was prepared hydrothermally in an autoclave from aqueous TiOCl2 solutions as a starting precursor. Titanate nanotubes were obtained when the sol–gel-derived TiO2 sol was treated chemically with a 10 M NaOH solution and subsequently heated in the autoclave at 150 °C for 48 h. The samples were characterized using XRD, TEM, SEM, EDX, Raman spectroscopy, and a BET surface area analyser. The effect of post treatments, such as washing with and without hydrochloric acid and calcination, on the phase structure, shape and morphology, pore structures, and BET surface area of the titanate nanotubes was investigated. When a sample containing 7.08 wt% Na (after washing only with water) was calcined at different temperatures from 300 to 900 °C, it showed the formation of a mixture of sodium trititanates and sodium hexatitanates and was found to preserve the tubular morphology at higher temperatures. However, a sample containing 0.06 wt% Na obtained after prolonged washing with hydrochloric acid followed by heat treatment showed the formation of TiO2 anatase involving TiO2 (B) as an intermediate at lower temperatures and anatase was further transformed to the rutile phase when the temperature was raised. On the basis of different observations, a general formula NaxH2−xTi3O7·nH2O has been proposed for the trititanate nanotubes.
ChemInform Abstract: Synthesis of Cyclopentenyl Sulfones via the (3 + 2) Cyclization‐Elimination Reaction of (Phenylsulfonyl)allene. (1988)

Abstract Reaction of dimethyl malonate (I) with the allene (II) in the presence of a trace of NaH gives the Michael‐type adduct (III) which rearranges to the thermodynamically more stable isomer (IV).
Recognition of Neurons Impulses with the Use of Nonlinear Dynamic Equations (2001)

We consider the problem of neurons impulses extracellular monitoring as a recognition problem of some specific segments of a signal  the impulses of one of neurons which appear at random time moments. For individual recognition the impulses of different neurons we use the impulses models as non-linear differential equations with a disturbance. These models enable to describe a signal disturbance not only by an amplitude but also by a phase. We have shown that the use of such models enables to reduce the problem to separation of a mixture of distributions close to normal in the transformed space of signs. We suggest and realize the algorithm of recognition of neurons impulses. The results of numerical experiment are cited.
Antenatally diagnosed thanatophoric dysplasia. (1982)

A case of antenatally diagnosed thanatophoric dysplasia is described. Other syndromes accompanied by chondrodysplastic tetramicromelia were excluded and diagnosis was based on the narrow thorax, secondary pulmonary hypoplasia and macrocephaly detected by ultrasound and on radiological findings of disturbed bone formation. At the mother's request labour was induced and radiological, anatomic and histological examination of the newborn confirmed the prenatal diagnosis. On basis of the literature, the possible aetiology of the disease is discussed and autosomal recessive heredity is suggested. Attention is focussed on the significant hydramnios which led to the suspicion of fetal malformation. The importance in pregnancy of routine ultrasound screening is emphasized, since such malformations can be detected as early as in midtrimester pregnancy.
JAK/STAT inhibition improves macrophage phagocytosis of bacteria (2011)

In COPD, the lower airways are colonised with bacteria that contribute to exacerbations. This may be due to defective macrophage phagocytosis. IFNγ is elevated in COPD and can suppress macrophage phagocytosis. IFNγ activates the JAK/STAT pathway, thus we hypothesise that inhibiting this pathway will ablate the IFNγ effect on macrophage phagocytosis. Human lung macrophages were treated with two structurally distinct JAK/STAT inhibitors (PF95 &amp; PF13, 10 -4 -10 -10 M), stimulated with 10ng/ml IFNγ for 18h. Phagocytosis was measured after 4h exposure to beads, H. influenzae (HI) or S. pneumoniae (SP) . CXCL10 and IL-6 release after IFNγ or IFNγ+TNFα (10ng/ml) stimulation for 24h was measured by ELISA n=5. Cell viability was assessed by MTT. IFNγ increased bead phagocytosis by 30±6% which was not altered by PF95 or PF13. However IFNγ significantly inhibited uptake of HI by 24±9% and SP by 29±9%. Both PF95 and PF13 reversed these effects in a concentration-dependent manner [Table 1]. PF13, but not PF95, was cytotoxic at concentrations ≥10μM. Both PF95 and PF13 decreased CXCL10 release after IFNγ stimulation (EC 50 0.31±0.17μM &amp; 15±9.5nM respectively) and IFNγ+TNFα (EC 50 0.57±0.2μM &amp; 19±8.2nM respectively). Only PF13 decreased IL-6 after IFNγ or IFNγ+TNFα stimulation (EC 50 0.45±0.72μM and 2.3±2.1μM respectively). Table 1. Effect of JAK/STAT inhibitors on IFNγ suppression of bacterial phagocytosis Drug HI SP PF95 EC 50 μM 0.27±0.26 0.21±0.19 % increase 39±16 62±15 PF13 EC 50 nM 2.5±2.4 12±15 % increase 36±17 37±12 Date are mean±SEM, n=5-6 Inhibiting the JAK/STAT pathway diminishes the decrease in phagocytosis of HI and SP caused by IFNγ and decreases the release of inflammatory cytokines and has potential as a novel target in COPD.
J044103 Sensorless Model-Free Vibration Control without Model of Controlled Object (2012)

This paper proposes a method of sensorless model-free vibration control based on DVFB (Direct Velocity Feedback). In general, model-based control theory is applied to design control system. Hence, an accurate vibration model of the system is required to achieve an adequate vibration control performance. However, making an accurate model and designing a control system increase the workload of jpnineers, and installing sensors to detect structural vibration obstructs a cost reduction of control system. In this paper, the present vibration control method is examined and the effectiveness is validated by simulation with target SDOF and MDOF structures.