gebhart/patrepeval huggingface dataset:
P04.15 Autophagy induction following TTFields application serves as a survival mechanism mediated by AMPK signaling (2018)

Tumor treating fields (TTFields) are an approved treatment modality for patients with glioblastoma, delivered via noninvasive application of low-intensity, intermediate-frequency, alternating electric fields. TTFields were shown to induce abnormal mitosis, aneuploidy, and increased cell granularity, which are often associated with enhancement of autophagy. In this work, we evaluated the possible effect of TTFields on regulation of autophagy in glioma cells Cells were treated with TTFields using the inovitroTM system. Cellular granularity was evaluated using flow cytometry. Autophagy was monitored by quantifying levels of lipidated Microtubule Associated Protein Light Chain 3 (LC3-II), in the presence and absence of the lysosomotropic agent and autophagy inhibitor chloroquine (CQ), using immunoblotting and immunofluorescence microscopy. Transmission Electron Microscopy (TEM) was used to visualize autophagosome-like structures. Time-lapse microscopy was utilized to follow autophagy induction in dividing cells. Endoplasmic reticulum (ER) stress markers were monitored by immunoblotting (for GRP78) and colorimetric assay (for intracellular ATP). Evaluating the roll of AMP activated protein kinase (AMPK) in autopagy induction was conducted by immunoblotting, as well as AMPK depletion by siRNA. Involvement of autophagy in cell fate after TTFields treatment was evaluated by producing Atg7 depleted glioma cell lines via shATG7 infection, as well as by combining TTFields with CQ, and monitoring cell growth and apoptosis by flow cytometry. Significant increase in cellular granularity was demonstrated in all tested cell lines. Up-regulation of autophagy in glioma cell lines treated with TTFields was demonstrated by immunoblot analysis. Fluorescence and transmission electron microscopy demonstrated the presence of LC3 puncta and typical autophagosome-like structures in treated cells. Time-lapse microscopy revealed significant increase in the formation of LC3 puncta, specific to cells which divided during treatment. Indication of proteotoxic stress in treated cells was evident based on elevated GRP78 levels and decreased cellular ATP. Pathway analysis showed that AMPK activation regulates autophagy induction by TTFields. AMPK depletion from U87-MG cells resulted in autophagy inhibition as reflected by LC3-II levels. Depletion of either AMPK or Atg7 sensitized cells to the treatment, suggesting that cancer cells utilize autophagy as a resistance mechanism to TTFields. Combining TTFields with CQ resulted in a significant dose-dependent reduction of cell growth compared with TTFields or CQ alone. These results suggest that dividing cells upregulate autophagy in response to aneuploidy and ER stress induced by TTFields application and that AMPK may serve as a key regulator of this process.
Mapping the Structural and Dynamical Features of Multiple p53 DNA Binding Domains: Insights into Loop 1 Intrinsic Dynamics (2013)

The transcription factor p53 regulates cellular integrity in response to stress. p53 is mutated in more than half of cancerous cells, with a majority of the mutations localized to the DNA binding domain (DBD). In order to map the structural and dynamical features of the DBD, we carried out multiple copy molecular dynamics simulations (totaling 0.8 μs). Simulations show the loop 1 to be the most dynamic element among the DNA-contacting loops (loops 1-3). Loop 1 occupies two major conformational states: extended and recessed; the former but not the latter displays correlations in atomic fluctuations with those of loop 2 (~24 Å apart). Since loop 1 binds to the major groove whereas loop 2 binds to the minor groove of DNA, our results begin to provide some insight into the possible mechanism underpinning the cooperative nature of DBD binding to DNA. We propose (1) a novel mechanism underlying the dynamics of loop 1 and the possible tread-milling of p53 on DNA and (2) possible mutations on loop 1 residues to restore the transcriptional activity of an oncogenic mutation at a distant site.
Under the Dome: “Chinese” smog as a viral media event (2016)

Under the Dome, a video about smog in China produced by author and former television anchor Chai Jing, garnered 200 million views within 48 hours before disappearing from major Chinese websites. This essay examines the video’s aesthetics, distribution and reception with a particular attention to its relation to time. I argue that a focus on temporality allows us to discern the ideological conditions that delimit how we talk about Under the Dome and indeed China’s pollution in the context of neoliberal globalization. The celebration of the video’s digitally enabled instantaneity not only privileges technological solutions and consumer agency but also obscures the unequal relation of power to which the Chinese state and the workers within the global electronics supply chain have long been subject. What these discourses eclipse is an opportunity to rethink the accidental event as opening an alternative dialogue about the geological deep time of media culture.
Identification and control of induction motor stator currents using fast on-line random training of a neural network (1997)

Artificial neural networks (ANNs), which have no off-line pretraining, can be trained continually on-line to identify an inverter-fed induction motor and control its stator currents. Due to the small time constants of the motor circuits, the time to complete one training cycle has to be extremely small. This paper proposes and evaluates a new form of the random weight change (RWC) algorithm, which is based on the method of random search for the error surface gradient. Simulation results show that the new form of the RWC, termed continually online trained RWC (COT-RWC), yields performance very much the same as conventional backpropagation with on-line training. Unlike backpropagation, however, the COT-RWC method can be implemented in mixed digital/analog hardware and still have a sufficiently small training cycle time. The paper also proposes a VLSI implementation which completes one training cycle in as little as 8 /spl mu/s. Such a fast ANN can identify and control the motor currents within a few milliseconds and, thus, provide self-tuning of the drive while the ANN has no prior information whatsoever of the connected inverter and motor.
Alzheimer's neurofibrillary tangles contain unique epitopes and epitopes in common with the heat-stable microtubule associated proteins tau and MAP2. (1987)

Ten monoclonal antibodies raised against Alzheimer's neurofibrillary tangles (ANTs) were characterized for reactivity with heat-stable microtubule fractions from bovine and human brain. Five of the antibodies showed very little reaction, but the other five reacted strongly with heat-stable microtubule associated proteins (MAPs). The proteins recognized by these antibodies have estimated molecular weights similar to those of known heat-stable MAPs, tau (52-68 kd) and MAP2 (200-250 kd). That the proteins are indeed tau and MAP2 is demonstrated by reaction of electroblotted proteins with antibodies raised in mouse and guinea pig against bovine brain tau and MAP2. One anti-ANT antibody reacts only with tau, two bind strongly to tau and weakly to MAP2, one recognizes both tau and MAP2 equally well, and one primarily stains MAP2. Extraction of ANT with 2% SDS does not remove tau or MAP2 epitopes from ANT, indicating that epitopes shared with heat-stable MAPs are integral components of ANT. The existence of tau epitopes in ANT is also demonstrated by immunoblotting of ANT-enriched fractions with anti-tau antibodies. Most of the material recognized by anti-tau antibodies in ANT-enriched fractions is present in large molecules excluded by 3% polyacrylamide gel upon electrophoresis. Anti-tau antibodies immunostain ANT in immunofluorescence and immunoperoxidase studies. The immunostaining can be blocked by absorption of anti-tau antibodies with purified tau proteins from bovine brain. Not all ANTs in any given tissue section or isolated Alzheimer perikarial preparations, however, are stained by anti-tau antibodies. These results are consistent with previous studies that have demonstrated heterogeneity of ANTs. Whether this heterogeneity is due to biochemical modification of MAPs or absence of MAPs in some ANTs is unknown. The significance of what appear to be shared epitopes recognized by monoclonal antibodies in tau and MAP2, and the implications this may have on the pathogenesis of ANT formation, requires further investigation.
Abstract 16 (2015)

AMERICAN SOCIETY OF PLASTIC SURGEONS PLASTIC & RECONSTRUCTIVE SURGERY PRS GLOBAL OPEN ASPS EDUCATION NETWORK AMERICAN SOCIETY OF PLASTIC SURGEONS PLASTIC & RECONSTRUCTIVE SURGERY PRS GLOBAL OPEN ASPS EDUCATION NETWORK
Notes - A New Method for the Preparation of Formamidine (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNotes - A New Method for the Preparation of FormamidineKeijiro OdoKeijiro OdoMore by Keijiro Odo, Eiichi IchikawaEiichi IchikawaMore by Eiichi Ichikawa, Kozo ShiraiKozo ShiraiMore by Kozo Shirai, and Kiichiro SuginoKiichiro SuginoMore by Kiichiro SuginoCite this: J. Org. Chem. 1957, 22, 12, 1715Publication Date (Print):December 1, 1957Publication History Published online9 April 2003Published inissue 1 December 1957https://pubs.acs.org/doi/10.1021/jo01363a620https://doi.org/10.1021/jo01363a620research-articleACS PublicationsRequest reuse permissionsArticle Views330Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Steroids and Sterols in Umbilical Cord Blood1 (1965)

An investigation of 2 pools of umbilical cord blood by means of paper, column, thin-layer and gas-liquid chromatography has led to the detection of 23 steroids and of 5 sterols, the criteria for the identification of each of which are specified. The majority of these compounds appeared to exist in blood as sulfo-conjugates, recoverable by a transesterification procedure. The majority of the steroids and all 5 sterols were found to be digitonin-precipitable, Δ5-3β-hydroxy compounds. These findings, and the results of experiments designed to measure total plasma Δ5-3β-hydroxysteroids and ketosteroids in young infants, are interpreted to indicate that the origin of the Δ5-3β-hydroxysteroids detected in umbilical cord blood is the fetal zone of the adrenal cortex. The fetal zone appears to be markedly deficient in 3β-hydroxysteroid dehydrogenase activity. The relationship of this enzymatic deficiency to cortisol synthesis and of cortisol metabolism to the intrauterine development of the fetal zone of the adrenal are discussed
DC Modeling of Composite MOS Transistors (1995)

Mixed-signal circuit design on sea-of-gates arrays requires the use of composite MOSTs, combinations of in-series and in-parallel connected unit MOSTs. To avoid an increase in circuit simulation complexity these are in general replaced by artificial single MOSTs. The analysis in this paper shows that a straightforward replacement will lead to incorrect results. Series MOSTs (in-series connected unit MOSTs) are essentially different from single MOSTs due to the presence of diffusion areas interrupting the channel at regular distances. The influence of lateral diffusion, charge sharing, and series resistance needs to be reconsidered. The theoretical results are confirmed by measurements on an experimental IC. Parameter decks of existing MOST models for circuit level simulation can be modified easily to reflect the length dependences of composite MOST parameters.
Partial characterization of apoptotic factor in Alzheimer plasma (1999)

We have previously demonstrated that a plasma natriuretic factor is present in Alzheimer's disease (AD), but not in multi-infarct dementia (MID) or normal controls (C). We postulated that the natriuretic factor might induce the increased cytosolic calcium reported in AD by inhibiting the sodium-calcium antiporter, thereby activating the apoptotic pathway. To test for a factor in AD plasma that induces apoptosis, we exposed nonconfluent cultured LLC-PK1 cells to plasma from AD, MID, and C for 2 h and performed a terminal transferase-dUTP-nick-end labeling (TUNEL) assay. The plasma from AD increased apoptosis nearly fourfold compared with MID and C. The effect was dose dependent and the peak effect was attained after a 2-h exposure. Additionally, apoptotic morphology was detected by electron microscopy, and internucleosomal DNA cleavage was found. We inhibited apoptosis by removing calcium from the medium, inhibiting protein synthesis with cycloheximide, alternately boiling or freezing and thawing the plasma, and digesting a partially purified fraction with trypsin. Heating AD plasma to 56 degrees C did not deactivate the apoptotic factor. These results demonstrate the presence of an apoptotic factor in the plasma of patients with AD.
Electrical and electrochemical properties of TiO2 films grown by organometallic chemical vapour deposition (1982)

The electrical and photoelectrochemical properties of TiO2 films, which were obtained by the vapour decomposition of ethyl titanate, are described. The electrical properties depend mainly on the deposition temperature. Films deposited at high temperatures have much lower electrical conductivity but remarkably higher photosensitivity than those deposited at low temperatures. The deposition temperatures are found to affect the electrochemical properties, such as anodic photocurrent–potential characteristics and spectra dependence of anodic photocurrents. Some doping effects of Al, Cr and Fe on the both properties are also examined.
Supplementary Table 1 from The Pervasive Presence of Fluctuating Oxygenation in Tumors (2023)

Supplementary Table 1 from The Pervasive Presence of Fluctuating Oxygenation in Tumors
Preliminary Experience Using a Computer-mediated Flexible Circular Stapler in Laparoscopic Esophagogastrostomy (2008)

Intracorporeal esophagogastrostomy after laparoscopic proximal gastrectomy is technically challenging. We employed a computer-mediated flexible circular stapler (SurgASSIST) for esophagogastrostomy in 2 cases with gastrointestinal stromal tumor located near the esophagogastric junction. Esophagogastrostomy was successfully constructed intracorporeally using the double-stapling technique. Operation times for the 2 cases were 225 and 170 minutes. No anastomotic leakage was encountered. However, anastomotic stricture requiring balloon dilatation occurred in 1 patient. The SurgASSIST system was feasible for esophageal anastomosis in laparoscopic proximal gastrectomy. However, the digital loading unit (DLU) is too large to introduce transorally, and attempting introduction of the DLU through the narrow lumen may create lesions or perforate the organ. Further improvements in the DLU will facilitate wider use of this system for various procedures in laparoscopic surgery.
Nonlinear Self-Injection Locking: Theory and Experiment (2020)

The impact of microresonator nonlinearity on the self-injection locking is studied. The theoretical model is developed and confirmed experimentally using a DFB diode locked to a chip-based microresonator. Nontrivial dynamics is uncovered.
Dynamic Policy Attribute Based Encryption and its Application in Generic Construction of Multi-Keyword Search (2019)

Attribute based encryption (ABE) is an encryption technique which provides a good solution to the security issues in the cloud environment. Through ABE, a data owner can achieve the fine-grained sharing of data encrypted under attributes or an access policy which they possess. The relation among these attributes is represented by the access policy which is expressed as an access tree. In this article, the authors first present an ABE scheme which supports frequent changes in the access tree and hence, it is named a dynamic policy ABE. Also, the proposed scheme generates secret keys of constant size which can save bandwidth. The proposed scheme is based on key-policy design and supports monotonic access structure that consists of AND, OR and Threshold gates. Inspired by the proposed dynamic policy ABE scheme the authors then present a multi-keyword search scheme which inherits all the features of the proposed ABE scheme. Therefore, it provides a constant size trapdoor and support for fast search. The construction of a multi-keyword search scheme is generic in nature and any ABE scheme can be converted to the multi-keyword search scheme using the transformation method given in the paper. Finally, the proposed schemes are proven to be secure under Decisional Bilinear Diffie-Hellman (DBDH) assumption.
Supplementary Table S4 from SLFN11 is Widely Expressed in Pediatric Sarcoma and Induces Variable Sensitization to Replicative Stress Caused By DNA-Damaging Agents (2023)

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Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells (2016)

Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality.
10-MHz photon counting detector system for time-resolved x-ray diffraction (1995)

We present the first results from two highly parallel detector systems designed for fast time resolved x-ray diffraction experiments. The readout systems have been designed to give throughputs well in excess of 10<SUP>7</SUP> events per second. The detector systems have been designed to allow high flux diffraction patterns to be collected with very much reduced rate effects when compared with previous designs. This has been achieved using wire microgap proportional counters coupled to multi-channel data acquisition systems. The efficiency and low noise of the detector coupled to the speed of the readout has produced a detector system capable of more fully exploiting the time resolved diffraction stations on the UK Synchrotron Radiation Source at the Daresbury Laboratory. Modifications to the design will be presented which will allow the system to cope with even higher count rates in the future.
Studies on the Morphine Alkaloids. X (1964)

Reduction of the enamine (I) of the 6-ketone compounds of morphine alkaloid with sodium borohydride and formic acid and also its catalytic reduction were examined. Catalytic reductive amination of the 6-ketone compounds (IV, V, VI) was also carried out.
Innovative Composite Slab System with Integrated Installation Floor (2009)

The paper presents a novel type of composite floor system using cellular beams. A major benefit of the system is the integrated installation floor, providing additional value to the floor. The system is based on half cellular beams made of existing hot-rolled sections. The openings in the cellular beams allow placing installations in all directions, thus providing excellent flexibility to the user when maintaining or changing installations. The paper first presents the general design and details of the construction of the novel floor system. The results of tests on the dynamic behaviour and the load-carrying capacity are then presented and compared with common calculation models for composite slabs. Finally, the paper presents an example of a first building where this type of floor system will be used.
A tRNAGlu that uncouples protein and tetrapyrrole biosynthesis (2005)

Glu-tRNA is either bound to elongation factor Tu to enter protein synthesis or is reduced by glutamyl-tRNA reductase (GluTR) in the first step of tetrapyrrole biosynthesis in most bacteria, archaea and in chloroplasts. Acidithiobacillus ferrooxidans, a bacterium that synthesizes a vast amount of heme, contains three genes encoding tRNA(Glu). All tRNA(Glu) species are substrates in vitro of GluRS1 from A. ferrooxidans.Glu-tRNA(3)(Glu), that fulfills the requirements for protein synthesis, is not substrate of GluTR. Therefore, aminoacylation of tRNA(3)(Glu) might contribute to ensure protein synthesis upon high heme demand by an uncoupling of protein and heme biosynthesis.
The impact of genetic markers on selection. (1998)

Genetic marker technologies, such as marker-assisted selection, parentage identification, and gene introgression can be applied to livestock selection programs. Highly saturated genetic maps are now available for cattle, swine, and sheep to provide the genetic framework for developing MAS programs. These programs rely on three phases for commercialization of the technology: the detection phase, in which quantitative trait loci are located and their effects on the phenotype measured; the evaluation phase, in which the markers are evaluated in commercial populations; and the implementation phase, in which markers are combined with phenotypic and pedigree information in genetic evaluation for predicting the genetic merit of individuals within the population. Predicting the economic impact of genetic technologies is a complex process that requires quantitative prediction and economic analysis. Evaluating the impact of these benefits across an industry can be achieved through a process in which gains from implementation of a genetic technology are assessed at the individual, enterprise, and industry levels. A pattern of annual benefits and costs can be predicted using gene flows that can be evaluated by conventional economic analysis.
Dual Rampart Antenna for Radar Systems with Enlarged Field of View (2022)

This paper presents a novel antenna based on a dual rampart radiating structure whose main radiation lobe can be steered across a broad angular range by changing the frequency of the input signal. The antenna characteristics are controlled electronically using suitable single-pull double-throw switches. The design has been completed using a full-wave electromagnetic simulation tool. A field of view of 135 degrees over the frequency band from 7 GHz to 8.5 GHz has been achieved.