gebhart/patrepeval huggingface dataset:
Ragweed Sensitization—induced Increase of Myosin Light Chain Kinase Content in Canine Airway Smooth Muscle (1992)

Previous studies have identified changes of mechanical properties of airway smooth muscle (ASM) from a canine model of atopic airway hyperreactivity. These changes, including increased maximum shortening capacity (delta Lmax) and early shortening velocity (Vo), may be responsible for the airway hyperresponsiveness in asthma. We have suggested that these changes may be due to increased actomyosin ATPase activity, controlled via phosphorylation of the 20 kD myosin light chain (MLC20) by MLC kinase (MLCK). Therefore, ATPase activity, MLC20 phosphorylation, and MLCK content and activity were assessed in tracheal and bronchial smooth muscles (TSM and BSM) of ragweed pollen-sensitized dogs (S) and their littermate controls (C). Specific ATPase activities from STSM and SBSM were significantly higher than their control counterparts (CTSM, CBSM). Phosphorylation of MLC20 in STSM was greater both at rest and during electrical stimulation due to the increased amount of MLCK in STSM and SBSM by 30 and 25%, respectively. MLCK activity was also increased significantly in STSM and SBSM (from 46.99 +/- 8.33 and 42.85 +/- 5.92 to 91.9 +/- 6.43 and 64.12 +/- 7.88 32P mmol/mg fresh tissue weight/min respectively [mean +/- SEM]). When normalized to the amount of MLCK in the tissue, however, specific MLCK activity in STSM and SBSM was similar to that in controls. It is unlikely that myosin phosphatase plays any role in the changes of MLC20 phosphorylation in sensitized animals. Peptide mapping showed no visible change in primary structure of MLCK in STSM and SBSM compared with those of controls. We report that ASM actomyosin ATPase activity is increased in STSM and SBSM. The increased ATPase activity is the result of increased MLC20 phosphorylation, the latter likely resulting from the increased MLCK content, which may account for the hyperresponsiveness found in ASM from these animals.
Both antisense and sense expression of biotin carboxyl carrier protein isoform 2 inactivates the plastid acetyl‐coenzyme A carboxylase in Arabidopsis thaliana (2002)

To further characterize the role of biotin carboxyl carrier protein isoform 2 (BCCP2) in acetyl-coenzyme A carboxylase (ACCase) function and fatty acid biosynthesis, plants with reduced or increased expression of this protein were characterized. Analysis of 38 independent Arabidopsis lines expressing antisense BCCP2 transcript behind a constitutive promoter showed no significant phenotype, though antisense transcript was highly expressed. In developing seed, BCCP2 protein was reduced by an average of 38% resulting in a 9% average decrease in fatty acid content in mature seed. Over-expression of BCCP2 behind a seed-specific napin promoter increased the amount of holo-BCCP2 in developing seed by an average of twofold, as determined with anti-biotin antibodies. Surprisingly, the average fatty acid content of T2 seed from over-expression lines was 23% lower than wild-type seed. These plants also exhibited reduced seed setting in 18 of 20 T1 lines which was coincident with increased individual seed mass. Quantification of total BCCP2 in developing siliques using anti-BCCP2 antibodies indicated that as much as 60% of total plastidial BCCP2 was in the non-biotinylated form (apo-BCCP2). Although apo-BCCP2 was highly over-expressed in developing seed, accumulation of other ACCase subunits was unaffected. The specific activity of ACCase was up to 65% lower in developing seed of over-expression lines versus wild type. This was attributed to the assembly of biologically inactive (non-biotinylated) ACCase complexes. Consistent with ACCase exerting control over de novo fatty acid synthesis, reduced activity in developing seed resulted in lower oil content, altered fatty acid composition and reduced seed setting.
Synthesis, Spectroscopic, and Antimicrobial Study of Binary and Ternary Ruthenium(III) Complexes of Ofloxacin Drug and Amino Acids as Secondary Ligands (2020)

This article aimed to synthesis, spectroscopic, physicochemical characterizations, and catalytic studies on some ofloxacin (OFL) complexes with ruthenium(III) [Ru(III)] metal ions. The Ru(III)-OFL complex, [Ru(OFL)2(Cl)2]Cl and two mixed-ligand complexes, [Ru(OFL)(AA)(H2O)2]Cl2 derived from OFL as the primary ligand and amino acids [AA; either glycine (Gly) or alanine (Ala)] as the secondary ligands, were synthesized and characterized using microanalytical, spectroscopic, and physical techniques including element composition, molar conductivity, infrared, electronic spectra, thermal, X-ray powder diffraction (XRD), Energy dispersive X-ray spectroscopy (EDX), Scanning electron microscopy (SEM), and Transmittance electron microscopy (TEM) analyses. The element analysis data describe the formation of 1:2 [Ru(III):OFL] and 1:1:1 [Ru(III):OFL:AA] complexes. The octahedral geometry of the complexes was confirmed by their magnetic moment and diffused reflectance. Fourier Transform Infrared spectroscopic (FTIR) measurements suggested that the ligands chelated with Ru(III) ions through the nitrogen atoms of the piperazine ring. In vitro antibacterial efficiency of the OFL compounds was evaluated by paper disc diffusion method. Significant antimicrobial activities were observed for some compounds of the series.
An Energy Efficient Autopilot Design (2020)

Abstract. In this paper, we propose a new control method for the next generation of sailboat autopilots. These new systems will need to manage more actuators to control the hydrofoils, which is going to significantly increase the energy requirements. So, this method is aware of the autopilot power consumption. It uses a model predictive controller to manage the actuators. This controller uses a dynamic model of the actuator, running in real time, to anticipate the future behavior of the system. Once the predictions are made, it determines the future control sequence to apply in order to follow the reference trajectory. To do so, it minimizes a cost function which takes into account two criteria: the precision of the system and the energy. With the proposed control method, skippers can focus on one or the other criterion depending on their goals and the boat's energy balance. We apply this method to one of the autopilot's subsystems, namely the rudder control. The electric actuator intervening in this control loop and the load representing the force opposed to its motion are modelled to design the control law. The first results of that method are compared with a standard autopilot. We increase by 40% the precision level and we are able to reduce the consumption by at least 20%. This work provides the first necessary components of a future autopilot that will control the whole appendages to a three-dimensional piloting. Moreover, this type of management is a first step towards possible fossil fuel-free sailboats.
RF MEMS-reconfigurable architectures for very large reflectarray antennas (2009)

The problem of very large reconfigurable reflectarray (RA) antennas based on RF MEMS technology is addressed in connection with a 77 GHz RA for imaging application being developed within the European FP7 project called ARASCOM. Since thousands of reconfigurable elementary cells are employed, reducing the number of tuneable components is of paramount importance for the practical realization of the reflectarray. A dramatic reduction of the number of tuneable devices is shown to be achievable without performance degradation by a proper architecture based on 1-bit digital phase shifting and on the separable illumination of the RA. The elementary cell design, the fabrication technology and the control network architecture are illustrated in some detail showing the feasibility of the approach proposed.
Choline kinase alpha impairment overcomes TRAIL resistance in ovarian cancer cells (2021)

Our findings suggest that ChoKα/CHKA impairment, by restoring drug-induced or receptor-mediated cell death, could be a suitable therapeutic strategy to be used in combination with chemotherapeutics or immunomodulators to improve OC patients' outcome.
New Insights into Sexual Dimorphism during Progression of Heart Failure and Rhythm Disorders (2010)

The mechanisms linking intrauterine growth retardation (IUGR) with adulthood obesity and diabetes are unclear. These studies investigated energy homeostasis in 8-and 20-wk-old male and female mice subjected to protein deficiency in utero. Pregnant C57BL/6J female mice were fed a protein-deficient diet (6% protein). Undernourished offspring (UO) and controls (CO) were cross-fostered to lactating dams fed a 20% control diet. The 24-h profiles of energy expenditure, feeding behavior, physical activity, and whole-body substrate preference was assessed using 8-wk UO and CO weaned onto control diet. Blood chemistries, glucose tolerance, and expression of genes involved in hepatic lipid and glucose metabolism were analyzed in 8-and 20-wk-old CO and UO fed control or a high-fat diet. UO exhibited IUGR with catch-up growth at 8 wk of age and increased severity of diet-induced obesity and insulin resistance by 20 wk of age. Therefore, fetal malnutrition in the C57BL/6J mouse increases sensitivity to diet-induced obesity. Abnormal daily rhythms in food intake and metabolism, increased lipogenesis, and inflammation preceded obesity in the UO group. Arrhythmic expression of circadian oscillator genes was evident in brain, liver, and muscle of UO at 8 and 20 wk of age. Expression of the clock-associated nuclear receptor and transcription repressor Rev-erb was reduced in liver and muscle of UO. Altered circadian physiology may be symptomatic of the metabolic dysregulation associated with IUGR, and altered feeding behavior and substrate metabolism may contribute to the obese phenotype.
Associations of age-dependent IGF-I SDS with cardiovascular diseases and risk conditions: cross-sectional study in 6773 primary care patients (2008)

The findings suggest the existence of multiple and complex interactions between IGF-I and several health conditions. The complex nature of disease- and subgroup-specific associations along with the methodological factors can be held responsible for divergent findings in previous studies.
By-products from integrated starch-ethanol production from barley in the diets of growing cattle (1989)

Two experiments were carried out to evaluate the nutritive value for growing cattle of by-products from a new process for integrated starch-ethanol production from barley. Experiment 1 was a 4 x 4 Latin Square, in which the effects of barley protein (375 g crude protein (CP)/kg dry matter (DM)) on digestibility and N retention were examined in four male cattle (initial live weight(LW) 147 kg). The control diet (C) consisted of hay and rolled barley (1 : 1). In isonitrogenous experimental diets, soybean meal (S), a mixture of soybean meal and barley protein (SB) or barley protein (B) was substituted for barley to increase the dietary CP content from 125 to 150 g/kg DM. Replacement of barley with protein supplements increased (P
Supplementary Methods and Figure Legend from Overexpression of Protease-Activated Receptor-1 Contributes to Melanoma Metastasis via Regulation of Connexin 43 (2023)

Supplementary Methods and Figure Legend from Overexpression of Protease-Activated Receptor-1 Contributes to Melanoma Metastasis via Regulation of Connexin 43
Application of Stool-PCR test for diagnosis of Helicobacter pylori infection in children (2009)

Association between higher score of H pylori in histology and a positive stool-PCR make it a very useful test for detection of H pylori active infection in children. We also suggest that a simple stool-PCR method can be a useful test for detection of H pylori virulence genes in stool.
Synthesis and reactions of anionic phosphide and arsenide complexes derived from disodium tetracarbonylferrate(-II) (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and reactions of anionic phosphide and arsenide complexes derived from disodium tetracarbonylferrate(-II)James P. Collman, R. G. Komoto, and W. O. SieglCite this: J. Am. Chem. Soc. 1973, 95, 7, 2389–2390Publication Date (Print):April 1, 1973Publication History Published online1 May 2002Published inissue 1 April 1973https://pubs.acs.org/doi/10.1021/ja00788a062https://doi.org/10.1021/ja00788a062research-articleACS PublicationsRequest reuse permissionsArticle Views105Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Genesis of Basic and Multi-Layer Echo State Network Recurrent Autoencoders for Efficient Data Representations (2018)

It is a widely accepted fact that data representations intervene noticeably in machine learning tools. The more they are well defined the better the performance results are. Feature extraction-based methods such as autoencoders are conceived for finding more accurate data representations from the original ones. They efficiently perform on a specific task in terms of 1) high accuracy, 2) large short term memory and 3) low execution time. Echo State Network (ESN) is a recent specific kind of Recurrent Neural Network which presents very rich dynamics thanks to its reservoir-based hidden layer. It is widely used in dealing with complex non-linear problems and it has outperformed classical approaches in a number of tasks including regression, classification, etc. In this paper, the noticeable dynamism and the large memory provided by ESN and the strength of Autoencoders in feature extraction are gathered within an ESN Recurrent Autoencoder (ESN-RAE). In order to bring up sturdier alternative to conventional reservoir-based networks, not only single layer basic ESN is used as an autoencoder, but also Multi-Layer ESN (ML-ESN-RAE). The new features, once extracted from ESN's hidden layer, are applied to classification tasks. The classification rates rise considerably compared to those obtained when applying the original data features. An accuracy-based comparison is performed between the proposed recurrent AEs and two variants of an ELM feed-forward AEs (Basic and ML) in both of noise free and noisy environments. The empirical study reveals the main contribution of recurrent connections in improving the classification performance results.
Adipocyte Xbp1s overexpression drives uridine production and reduces obesity (2018)

These observations reveal that Xbp1s is a potent stimulator of uridine production in adipocytes, enhancing lipolysis and invoking a potential anti-obesity strategy through the induction of a futile biosynthetic cycle.
Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog (2015)

In LeuT, a prokaryotic homolog of neurotransmitter transporters, Na(+) stabilizes outward-open conformational states. We examined how each of the two LeuT Na(+) binding sites contributes to Na(+)-dependent closure of the cytoplasmic pathway using biochemical and biophysical assays of conformation. Mutating either of two residues that contribute to the Na2 site completely prevented cytoplasmic closure in response to Na(+), suggesting that Na2 is essential for this conformational change, whereas Na1 mutants retained Na(+) responsiveness. However, mutation of Na1 residues also influenced the Na(+)-dependent conformational change in ways that varied depending on the position mutated. Computational analyses suggest those mutants influence the ability of Na1 binding to hydrate the substrate pathway and perturb an interaction network leading to the extracellular gate. Overall, the results demonstrate that occupation of Na2 stabilizes outward-facing conformations presumably through a direct interaction between Na(+) and transmembrane helices 1 and 8, whereas Na(+) binding at Na1 influences conformational change through a network of intermediary interactions. The results also provide evidence that N-terminal release and helix motions represent distinct steps in cytoplasmic pathway opening.
ChemInform Abstract: Microwave Heating in Solid‐Phase Peptide Synthesis (2012)

Abstract Review: 254 refs.
Software support for building end-user programming environments in the automation domain (2008)

Projects in the automation domain often require that end users, who are the machine operators, have means to change control software to make adaptations and optimizations for the machining task at hand. Although they usually do not have any software development expertise, they intervene in safety-critical software systems. This results in high demands on end-user programming environments with respect to supporting, guiding, and supervising end users. In this paper we present a software framework which is intended to serve as a basis for developing end-user programming environments. The main parts of this framework are a domain-specific language for programming automation solutions at a high level of abstraction, different visual editors for supporting end users, an approach for checking program changes against formal specifications, a variability modeling approach for representing high-level user decisions, and an approach for setting up customized end-user environments from models.
Contact Aided Compliant Mechanisms: Concept and Preliminaries (2002)

Compliant mechanisms (CMs) are the focus of active research because of the scalability, robustness and ease of manufacture endowed by their unitized construction. However, despite significant advances in development of systematic design techniques for these mechanisms, currently CMs are not capable of performing certain kinematic tasks that rigid body mechanisms can readily perform. This paper explores the use of contact interactions to enhance the functionality of CMs and thus widen their scope of application. Unlike CM designs without contact interactions, contact aided compliant mechanisms (CCMs) can be designed to generate non-smooth output port trajectories with a single continuous input. CCMs can also be designed to generate non-smooth force-deflection characteristics within the small deformation bounds imposed by linear elasticity. A wide variety of contact interactions, from the simple case involving a single point contact of a CM with a rigid external body to the more complex case of multiple contacts between different parts of the CM itself, can be used creatively to bridge the gap between the capabilities of CMs and conventional rigid body mechanisms. Several novel CCM designs that demonstrate useful non-smooth output trajectories are fabricated and tested. This paper also explores the use of optimization as a tool for systematic synthesis of CCMs. A simple one dimensional example is used to illustrate the use of topology optimization for systematic synthesis of a CCM for non-smooth force-deflection characteristics.
Crossed-beam reaction of carbon atoms with hydrocarbon molecules. I. Chemical dynamics of the propargyl radical formation, C3H3 (X2B2), from reaction of C(3P j) with ethylene, C2H4(X1A g) (1996)

The reaction between ground-state carbon atoms, C(3Pj), and ethylene, C2H4(X1Ag), was studied at average collision energies of 17.1 and 38.3 kJmol−1 using the crossed molecular beams technique. Product angular distributions and time-of-flight spectra of m/e=39 were recorded. Forward-convolution fitting of the results yields a maximum energy release as well as angular distributions consistent with the formation of the propargyl radical in its X2B2 state. Reaction dynamics inferred from the experimental data indicate two microchannels, both initiated by attack of the carbon atom to the π-orbital of the ethylene molecule via a loose, reactant like transition state located at the centrifugal barrier. Following Cs symmetry on the ground state 3A″ surface, the initially formed triplet cyclopropylidene complex rotates in a plane roughly perpendicular to the total angular momentum vector around its C-axis, undergoes ring opening to triplet allene, and decomposes via hydrogen emission through a tight transition state to the propargyl radical. The initial and final orbital angular momenta L and L′ are weakly coupled and result in an isotropic center-of-mass angular distribution. A second microchannel arises from A-like rotations of the cyclopropylidene complex, followed by ring opening and H-atom elimination. In this case, a strong L-L′ correlation leads to a forward-scattered center-of-mass angular distribution. The explicit identification of C3H3 under single collision conditions represents a single, one-step mechanism to build up hydrocarbon radicals. Our findings strongly demand incorporation of distinct product isomers of carbon atom-neutral reactions in reaction networks simulating chemistry in combustion processes, the interstellar medium, as well as in outflows of carbon stars, and open the search for the hitherto unobserved interstellar propargyl radical.
Deep trapping effects at the GaAs-GaAs : Cr interface in GaAs FET structures (1978)

Transient capacitance measurements were made in order to study the interface properties of FET-type devices with and without LPE buffer layers. No detectable traps were found in the buffered devices, whereas two deep hole traps, located 0.58 and 0.81 eV above the valence band, were detected in the unbuffered FET devices when the gate-depletion region approached the substrate interface. The trap concentrations were 1×1016 and 2×1015 cm−3, respectively. An interface model was developed and used to show that the minority-carrier trapping effects which appeared in the active layer are actually due to the image effect of majority-carrier traps in the Cr-doped substrate acting through the interface space-charge region.
Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p (2001)

Nuclear export of ribosomes requires a subset of nucleoporins and the Ran system, but specific transport factors have not been identified. Using a large subunit reporter (Rpl25p-eGFP), we have isolated several temperature-sensitive ribosomal export (rix) mutants. One of these corresponds to the ribosomal protein Rpl10p, which interacts directly with Nmd3p, a conserved and essential protein associated with 60S subunits. We find that thermosensitive nmd3 mutants are impaired in large subunit export. Strikingly, Nmd3p shuttles between the nucleus and cytoplasm and is exported by the nuclear export receptor Xpo1p. Moreover, we show that export of 60S subunits is Xpo1p dependent. We conclude that nuclear export of 60S subunits requires the nuclear export sequence-containing nonribosomal protein Nmd3p, which directly binds to the large subunit protein Rpl10p.
Biomechanical regulation of tumor multicellular spheroid growth and cell invasion (2014)

International audience