gebhart/patrepeval huggingface dataset:
A Juxtacrine Mechanism for Neutrophil Adhesion on Platelets Involves Platelet-Activating Factor and a Selectin-Dependent Activation Process (1998)

The aim of this study was to identify the molecular mechanisms involved in neutrophil adhesion to immobilized platelets with particular focus on the possible existence of a juxtacrine system for neutrophil-platelet interactions. Platelets were immobilized onto collagen (type I)-coated coverslips that were placed in a flow chamber and neutrophils were perfused across these confluent monolayers at a shear stress of 1 to 4 dynes/cm2. Neutrophils rolled, and a significant proportion (25% to 50%) adhered to platelet monolayers. P-selectin was expressed in very large quantities on the surface of platelets and mediated all of the rolling, whereas the beta2-integrin mediated firm adhesion. An activation mechanism for adhesion was necessary inasmuch as fixed neutrophils continued to roll on immobilized platelets, but did not adhere. Platelets adherent to collagen produced significant levels of platelet-activating factor (PAF). Accordingly, the firm adhesion of neutrophils to platelets was significantly inhibited by a PAF receptor antagonist (WEB 2086). Treatment of only the platelets with acetylhydrolase, which converts membrane-associated PAF to lyso-PAF, prevented 60% of the adhesion. These data suggest that PAF, on the surface of platelets, mediated a significant portion of the adhesive interaction. Addition of some selectin-binding carbohydrates (fucoidan or soluble SLEx analogs but not dextran sulfate) to the platelets caused rolling neutrophils to immediately adhere, an event that was not observed on histamine or thrombin-treated endothelium or P-selectin transfectants. These data support the view that a juxtacrine activation process exists on immobilized platelets for neutrophils. This process can be greatly enhanced on platelets and may involve a signaling mechanism through P-selectin.
Misleading meta-analysis (1995)

A meta-analysis of treatments in myocardial infarction published in 1992 retrospectively showed that streptokinase was associated with a highly significant fall in mortality by 1977, after inclusion of 15 trials.1 Thrombolysis was, however, not widely recommended until 10 years later—after the effect was confirmed in two mega trials.1 2 3 In the case of magnesium, a substantial fall in mortality was evident by 1990, after inclusion of seven trials. In 1993, based on an updated meta-analysis it was argued that magnesium treatment represented an “effective, safe, simple and inexpensive” intervention that should be introduced into clinical practice without further delay.4 The negative results of ISIS 4 (the fourth international study of infarct survival), published in last week's Lancet,5 have dealt a blow to enthusiasm for both magnesium and meta-analysis.6 As the findings of meta-analyses and systematic reviews are generally not tested in mega trials the situation regarding magnesium represents an opportunity to examine a false positive meta-analysis.

The table compares the meta-analyses of trials of magnesium and streptokinase after myocardial infarction. Trials were cumulatively included until the treatment effect was significant at P<0.001. For magnesium, seven small trials whose results were published in the 1980s were sufficient to establish the effect. Although trials were larger in the case of fibrinolytic treatment, twice as many studies and two decades were necessary to reach the same level of significance. Until recently it could …
resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission (2022)

Points for accumulation in nanoscale topography (PAINT) allows the acquisition of practically unlimited measurements in localisation microscopy. However, PAINT is inherently limited by unwanted background fluorescence at high probe concentrations, especially in large depth-of-field volumetric imaging techniques. Here we present reservoir-PAINT (resPAINT), in which we combine PAINT with active control of probe photophysics. In resPAINT, a ‘reservoir’ of non-fluorescent activatable probes accumulate on the target, which makes it possible to drastically improve the localisation rate (by up to 50-fold) compared to conventional PAINT, without any compromise in contrast. By combining resPAINT with large depth-of-field microscopy, we demonstrate volumetric super-resolution imaging of entire cell surfaces. We then generalise the approach by implementing multiple switching strategies, including photoactivation and spontaneous blinking. We also implement alternative volumetric imaging modalities including the double-helix pointspread function, the tetrapod point-spread function and singlemolecule light field microscopy. Finally, we show that resPAINT can be used with a Fab to image membrane proteins, effectively extending the operating regime of conventional PAINT to encompass a larger range of biological interactions.
Adeno‐associated virus 8‐mediated gene therapy for choroideremia: preclinical studies in in vitro and in vivo models (2014)

Abstract Background Choroideremia (CHM) is a slowly progressive X‐linked retinal degeneration that results in a loss of photoreceptors, retinal pigment epithelium and choroid. CHM , the gene implicated in choroideremia, encodes Rab escort protein‐1 (REP‐1), which is involved in the post‐translational activation via prenylation of Rab proteins. Methods We evaluated AAV8.CBA.hCHM, a recombinant adeno‐associated virus serotype 8 (rAAV8) vector, which targets retinal cells efficiently, for both therapeutic effect and safety in vitro and in vivo in a murine model. In vitro studies included western blot analyses and prenylation assays. In vivo studies included ophthalmoscopy, pupillometry, histology and immunofluorescence analysis. Results Infection with AAV8.CBA.hCHM induced the expression of REP‐1 protein in a dose‐responsive fashion. Transduction with AAV8.CBA.hCHM reverses the biochemical and pathogenetic defects in CHM both in vitro and in vivo and showed no safety concerns in the in vivo investigations performed in the present study. Conclusions AAV8 is a promising vector for human clin i cal gene therapy trials for choroideremia. Copyright © 2014 John Wiley &amp; Sons, Ltd.
Design and fabrication of materials with desired deformation behavior (2010)

This paper introduces a data-driven process for designing and fabricating materials with desired deformation behavior. Our process starts with measuring deformation properties of base materials. For each base material we acquire a set of example deformations, and we represent the material as a non-linear stress-strain relationship in a finite-element model. We have validated our material measurement process by comparing simulations of arbitrary stacks of base materials with measured deformations of fabricated material stacks. After material measurement, our process continues with designing stacked layers of base materials. We introduce an optimization process that finds the best combination of stacked layers that meets a user's criteria specified by example deformations. Our algorithm employs a number of strategies to prune poor solutions from the combinatorial search space. We demonstrate the complete process by designing and fabricating objects with complex heterogeneous materials using modern multi-material 3D printers.
Complex spatio-temporal distribution and genogeographic affinity of mitochondrial DNA haplogroups in 24,216 Danes (2017)

Mitochondrial DNA (mtDNA) haplogroups (hgs) are evolutionarily conserved sets of mtDNA SNP-haplotypes with characteristic geographical distribution. Associations of hgs with disease and physiological characteristics have been reported, but have frequently not been reproducible. Using 418 mtDNA SNPs on the PsychChip (Illumina), we assessed the spatio-temporal distribution of mtDNA hgs in Denmark in DNA isolated from 24,642 geographically un-biased dried blood spots (DBS), collected from 1981 to 2005 through the Danish National Neonatal Screening program. Geno-geographic affinity (ancestry background) was established with ADMIXTURE using a reference of 100K+ autosomal SNPs in 2,248 individuals from nine populations. The hg distribution was typically Northern European, and hgs were highly variable based on median-joining analysis, suggesting multiple founder events. Considerable heterogeneity and variation in autosomal geno-geographic affinity was observed. Thus, individuals with hg H exhibited 95 %, and U hgs 38.2 % - 92.5 %, Danish ancestry. Significant clines between geographical regions and rural and metropolitan populations were found. Over 25 years, macro-hg L increased from 0.2 % to 1.2 % (p = 1.1*E-10), and M from 1 % to 2.4 % (p = 3.7*E-8). Hg U increased among the R macro-hg from 14.1 % to 16.5 % (p = 1.9*E-3). Geno-geographic affinity, geographical skewedness, and sub-hg distribution suggested that the L, M and U increases are due to immigration. The complex spatio-temporal dynamics and geno-geographic heterogeneity of mtDNA in the Danish population reflect repeated migratory events and, in later years, net immigration. Such complexity may explain the often contradictory and population-specific reports of mito-genomic association with disease.
Electron Transfer in Monolayer Assemblies with Incorporated Ruthenium (II) Complexes (1977)

Abstract An arrangement for light induced vectorial charge separation is discussed in which the electron of the excited dye is transferred to an acceptor and the dye is recovered by electron tunneling through a high and narrow potential barrier. The possible relevance of the model in connection with photosynthesis is considered. Monolayers of Ru(II)‐bipyridine complexes with long chain hydrocarbon substituents were investigated which may be useful as component in such arrangements. The surface pressure area isotherms of the monolayers were measured for various ionic compositions of the subphase, and the results demonstrate a strong effect of these ions on the structure of the layers. The layers were deposited on different substrates. The luminescence and its change by contacting the sample with water and by subsequent drying were found to be strongly dependent on the architecture of the layer assembly. Attempts of a photochemical cleavage of water with these assemblies failed. The pH‐dependence of the absorption and the luminescence of a Ru(II) bipyridine‐dicarboxylic acid complex in solution is interpreted by assuming that the electron in the excited state is localized in one pyridine part of the substituted ligand, the conjugation with the second half of the bipyridine carboxylic acid being negligible. Monolayer assemblies for measuring the energy transfer from the ruthenium complex to an adequate energy acceptor and from an adequate energy donor to the ruthenium complex were investigated. The results demonstrate that the deactivation of the excited ruthenium complex occurs mainly by passing the luminescent state. Assemblies were investigated for measuring the electron transfer from the excited ruthenium complex to an appropriate electron acceptor positioned in the carboxylate portion at the same interface as the electron donor. With bipyridinium ions as acceptor the ruthenium complex luminescence is quenched at average distances between acceptor molecules of about 10 Å, while this distance is 30, 60 und 75 Å for different cyanine dyes used instead of the ruthenium complex. A correlation between this distance and the ionization energy in the excited state of the donor is observed.
Surface‐coil T1 images (1986)

A method for producing calculated T1 images with high signal-to-noise ratio and good spatial resolution using surface coils is presented. Separate transmit and receive rf coils with electronic decoupling are employed to insure uniform B1 excitation. The sensitivity profile of the receiver coil is normalized out of the image by the T1 calculation. T1 values determined by this method show close agreement with previously reported values.
Enhanced Photovoltaic Performance of Dye-Sensitized Solar Cells Using TiO2-Graphene Microplatelets Hybrid Photoanode (2015)

Hybrid photoanodes for dye-sensitized solar cells (DSSCs) were prepared by simple addition of graphene (GR) microplatelets to TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> nanoparticulate paste. Transmission electron microscopy was used to confirm the presence of GR in composite films after heating at 450°C for 30 min. TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /GR-based DSSCs were fabricated using an N749 photosensitizer. The UV-Visible absorption spectroscopy, photocurrent-voltage (I-V) characteristic, and electrochemical impedance spectroscopy measurements were carried out to characterize the cells. The results indicate that the GR/TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> photoanode improves the performance of the solar cell. This is because the GR/titania electrode accelerates electronic transportation and suppresses the charge recombination. Under optimal conditions, the solar cell based on GR/TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> shows power conversion efficiency (PCE) of 4.1%, which is about 30% greater than the cell based on the pristine TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> electrode (3.16%). The objective of this study is to develop a fast, cheap, and an effective means to increase the PCE of DSSCs. Density functional theory was used to calculate the bandgap of TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> and graphnene-TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> .
Comparison of Time Domain Hybrid PAM and DMT for Data Center Applications (2017)

In this publication, we investigate and compare two approaches for flexible transmission systems with intensity modulation and direct detection for inter data center communications, namely discrete multi-tone and time domain hybrid pulse amplitude modulation. Both transmission approaches were used to vary the data rate between 56 and 112 Gb/s with fine granularity. Vestigial side-band transmission was used in order to avoid the power fading effect. Inter-symbol interference due to chromatic dispersion was compensated using digital signal processing at the receiver side. In an experimental verification of the DMT setup, we successfully transmitted 56 Gb/s over 150 km SSMF without in-line amplification.
Vision loss in Australia (2005)

Vision loss in Australia is a much bigger problem than is usually recognised; 76% of low vision is caused by uncorrected refractive error or cataract, both readily treatable. However, the prevention and treatment of macular degeneration poses a major challenge.
Cyclic Temperature and Thermal Stress Fluctuations in Blanket Engineering Fusion Reactors (1982)

Cyclic thermal loads and stresses in two critical components of fusion reactors, including fusion-fission hybrids, are modeled and calculated. The two critical components are the solid wall adjacent to the fusion plasma (“first wall”) and the fissile fuel elements in the high-power density region of the blanket. These two components exemplify two limiting cases of thermal loading: The first-wall loads are generated by predominantly shallow energy deposition that may be approximated with a flux across the surface and the fuel elements loads are generated by volumetric heating.Two approaches are used to solve the heat conduction equation and to calculate the resulting stresses in terms of system parameters. The first is expansion into Fourier series and determination of periodic solutions; the second is analysis and superposition of single-pulse responses weighted with appropriate time delay and decay factors. Approximate closed-form expression for temperature excursions and thermal stresses are derived; these expressions may be evaluated conveniently and rapidly for comparison of different systems. The results provide a quantitative basis for trade-off studies and comparative assessments of different fusion reactor systems.
Exploration of the Ice Giant Systems: A White Paper for NASA's Planetary Science and Astrobiology Decadal Survey 2023-2032 (2020)

Ice giants are the only unexplored class of planet in our Solar System. Much that we currently know about these systems challenges our understanding of how planets, rings, satellites, and magnetospheres form and evolve. We assert that an ice giant Flagship mission with an atmospheric probe should be a priority for the decade 2023-2032.
Current drug and molecular therapies for the treatment of atrophic age-related macular degeneration: phase I to phase III clinical development (2017)

Age-related macular degeneration (AMD) is the leading cause of vision loss among the elderly. Atrophic AMD, including early, intermediate and geographic atrophy (GA), accounts for ~90% of all cases. It is a multifactorial degeneration characterized by chronic inflammation, oxidative stress and aging components. Although no FDA-approved treatment yet exists for the late stage of atrophic AMD, multiple pathological mechanisms are partially known and several promising therapies are in various stages of development. Areas covered: Underlying mechanisms that define atrophic AMD will help provide novel therapeutic targets that will address this largely unmet clinical need. The purpose of this paper is to review current promising drugs that are being evaluated in clinical trials. Because no pharmacological treatments are currently available for late stage of atrophic AMD, any new therapy would have extensive market potential. Expert opinion: The number of AMD patients is predicted to increase to ~30 million worldwide by 2020. In response to this enormous unmet clinical need, new promising therapies are being developed and evaluated in clinical trials. We propose that the assessment of novel interventions will also need to consider the genotypes of participants, as the benefit may be determined by polymorphisms in an individual's genetic background.
MODELING A NATIVE AMERICAN RUIN: DETERMINATION OF AN UPPER-BOUND EARTHQUAKE AT LOS ALAMOS NATIONAL LABORATORY (2000)

No abstract prepared.
Manipulation of exciton distribution for high-performance fluorescent/phosphorescent hybrid white organic light-emitting diodes (2017)

This review reports on the recent development of fluorescent/phosphorescent hybrid white organic light-emitting diodes.
Refining of Particulates at Stimuli‐Responsive Interfaces: Label‐Free Sorting and Isolation (2021)

Abstract The mechanism of separation methods, for example, liquid chromatography, is realized through rapid multiple adsorption‐desorption steps leading to the dynamic equilibrium state in a mixture of molecules with different partition coefficients. Sorting of colloidal particles, including protein complexes, cells, and viruses, is limited due to a high energy barrier, up to millions kT, required to detach particles from the interface, which is in dramatic contrast to a few kT for small molecules. Such a strong interaction renders particle adsorption quasi‐irreversible. The dynamic adsorption‐desorption equilibrium is approached very slowly, if ever attainable. This limitation is alleviated with a local oscillating repulsive mechanical force generated at the microstructured stimuli‐responsive polymer interface to switch between adsorption and mechanical‐force‐facilitated desorption of the particles. Such a dynamic regime enables the separation of colloidal mixtures based on the particle‐polymer interface affinity, and it could find use in research, diagnostics, and industrial‐scale label‐free sorting of highly asymmetric mixtures of colloids and cells.
Improving Oil-Rate Estimate in Capacitance/Resistance Modeling Using the Y-Function Method for Reservoirs Under Waterflood (2019)

Summary Capacitance/resistance modeling (CRM) is an empirical waterflood modeling technique based on the signal correlations between injection rates and gross production rates. CRM can satisfactorily estimate the gross (liquid) production rate. The oil-production-rate forecast is based on fitting the empirical oil fractional-flow model, the Leverett (1941) oil fractional-flow model, or the Koval (1963) model to the historical production data. We observed that the oil-production-rate forecast in this approach is less satisfactory. We propose a robust approach that combines CRM gross production prediction with a Buckley-Leverett displacement-theory-based waterflood analytical method—the Y-function method—to calculate the oil fraction flow and to improve the oil prediction capability. The analytical method is based on the results of the historical production performance of either an individual producer or a group of producers in a given area. By using this method, a better understanding can be developed about the production performance, such as the breakthrough time of injected water and possible operational issues, such as water channeling. The analytical model compares oil fractional flow and the cumulative gross production on the producers, yet the value of saturation is not required. As a result, the forecast of the oil-production rate becomes more convenient and straightforward. Sayarpour et al. (2009a) outlined field examples to compare the estimated oil production obtained using the current empirical oil fractional flow-model approach and the analytical Y-function method. The new method provided another effective way to calculate the oil rate in CRM. The results indicated that the new approach improved the accuracy of the oil-rate calculation and proved convenient in field applications. The objective of this study was not to regenerate the gross-rate forecast of CRM, but rather to improve the oil fractional-flow description and oil-production-rate forecast from the gross rate using the Y-function method.
Panel on accelerators and detectors in the 1950s (1989)

Jones: The discussions are organized as follows: first some remarks on the subject of particle accelerators in the 1950s, then some remarks on particle detectors. Although people like Luis Alvarez bridge both fields, generally the two fields are more or less discrete. Robert R. Wilson deals with aspects of particle accelerators from the Cornell perspective; in a separate chapter in this volume, Donald Kerst summarizes the MURA developments in the 1950s;* Robert Hofstadter discusses detectors, in particular the development of solid inorganic scintillators. Ugo Amaldi and Alvarez then take up other aspects of particle detectors. The panel concludes with a general discussion, including responses to questions from the floor.
Discitis following urinary tract infection manifesting as recurrent autonomic dysreflexia related to truncal movements in a person with tetraplegia (2020)

A 44-year-old male person with tetraplegia (C-5 AIS-A (American Spinal Cord Injury Association Standard Neurological Classification of Spinal Cord Injury Impairment Scale)) developed urinary tract infection and received appropriate antibiotic. Subsequently, he started sweating and shivering when he was sitting up; these symptoms resolved while lying on his back. Autonomic dysreflexia triggered by truncal movements continued to occur for 3 months. CT of the spine showed L5-S1 discitis. MRI of the spine showed diffuse marrow oedema in L5 and S1 vertebrae and a large abscess at L5/S1 level. Blood culture yielded <i>Serratia marcescens</i> sensitive to meropenem. Meropenem followed by ertapenem was given for 12 weeks. After 11 months, MRI showed resolution of discitis and epidural collection. The patient was able to sit up for 9 hours without developing autonomic dysreflexia. If a person with cervical spinal cord injury develops posture-related autonomic dysreflexia (eg, in sitting position, lying on sides or while hoisted), disco-vertebral pathology should be suspected.