gebhart/patrepeval huggingface dataset:
Bone histology in young adult osteoporosis. (1989)

Bone histology was quantitated in 10 osteoporotic patients aged between 17 and 51 years and in six healthy subjects aged between 23 and 43 years. The osteoporosis was of varying aetiology and was clinically stable. All patients were given tetracycline before biopsy and double tetracycline labelling was used in seven patients. Bone forming and resorbing surfaces were defined by the presence of osteoblasts and osteoclasts, respectively, which were identified by histochemical techniques. The associations between bone forming and resorbing surfaces were similar in patients and controls, though the range of values was wider in the patients than in the controls. Mineral apposition rate was normal in the osteoporotic patients, but there was a reduction in mineralising (tetracycline) surface, whether related to osteoid surface or to osteoblast surface. This did not indicate osteomalacia as the directly and indirectly measured mineralisation lag times were normal. The osteoid seams were thinner in osteoporotic patients than in controls. The data suggest that osteoclast and osteoblast numbers were normal in this group of osteoporotic patients but that the metabolic activity of osteoblasts was impaired.
Cardiovascular effects of morphine during hypothermia. (1989)

The cardiovascular effects of morphine were determined in anesthetized dogs at 37 and 30 degrees C. Intravenous (i.v., 1 mg/kg) but not intracisternal (i.c., 0.1 mg/kg) morphine produced a significant cardiovascular depression only at 30 degrees C. These effects were antagonized by i.v. naloxone (1 mg/kg) suggesting a peripherally mediated opiate mechanism. Moreover, hypothermia significantly increased plasma and cerebrospinal fluid morphine levels and enhanced the morphine-induced histamine release. The results suggest that opiates could have detrimental effects in patients with poor cardiovascular reserve in which high doses of opiates and hypothermia may be used concomitantly.
Effect of Annealing Temperature on the Structure and Magnetic Properties of Co_(0.8)Fe_(2.2)O_4/SiO_2 Nanocomposite Films (2015)

Co0.8Fe2.2O4/Si O2 nanocomposite films on Si(100) substrates were prepared by a sol-gel spin-coating technique. The effect of annealing temperature on the structure, morphology and magnetic properties of the nanocomposite films was investigated by X-ray diffraction, scanning electron microscopy and vibrating sample magnetometry, respectively. The results show that Co0.8Fe2.2O4 in nanocomposite films annealed at 750 ℃ exhibits a cubic spinel structure. The grain size and the lattice constant of Co0.8Fe2.2O4 of the nanocomposite films increase from 6 nm to 34 nm and from 0.836 9 nm to 0.839 5 nm as the annealing temperature increases from 750 ℃ to 1 100 ℃, respectively. The coercivity of Co0.8Fe2.2O4/Si O2 nanocomposite films firstly increases and then decreases with the increase of the annealing temperature, and the higher coercivity of the film annealed at 1 000 ℃ is 298.3 k A/m.
Essential anaplerotic role for the energy-converting hydrogenase Eha in hydrogenotrophic methanogenesis (2012)

Despite decades of study, electron flow and energy conservation in methanogenic Archaea are still not thoroughly understood. For methanogens without cytochromes, flavin-based electron bifurcation has been proposed as an essential energy-conserving mechanism that couples exergonic and endergonic reactions of methanogenesis. However, an alternative hypothesis posits that the energy-converting hydrogenase Eha provides a chemiosmosis-driven electron input to the endergonic reaction. In vivo evidence for both hypotheses is incomplete. By genetically eliminating all nonessential pathways of H(2) metabolism in the model methanogen Methanococcus maripaludis and using formate as an additional electron donor, we isolate electron flow for methanogenesis from flux through Eha. We find that Eha does not function stoichiometrically for methanogenesis, implying that electron bifurcation must operate in vivo. We show that Eha is nevertheless essential, and a substoichiometric requirement for H(2) suggests that its role is anaplerotic. Indeed, H(2) via Eha stimulates methanogenesis from formate when intermediates are not otherwise replenished. These results fit the model for electron bifurcation, which renders the methanogenic pathway cyclic, and as such requires the replenishment of intermediates. Defining a role for Eha and verifying electron bifurcation provide a complete model of methanogenesis where all necessary electron inputs are accounted for.
Oestrogen regulated mRNAs in the ZR-75-1 human breast cancer cell line (1988)

Conference Article| April 01 1988 Oestrogen regulated mRNAs in the ZR-75-1 human breast cancer cell line ROGER J. DALY; ROGER J. DALY 1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K. Search for other works by this author on: This Site PubMed Google Scholar DAVID L. MANNING; DAVID L. MANNING 1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K. Search for other works by this author on: This Site PubMed Google Scholar CHRIS D. GREEN CHRIS D. GREEN 1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1988) 16 (2): 169–170. https://doi.org/10.1042/bst0160169a Article history Received: September 23 1987 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation ROGER J. DALY, DAVID L. MANNING, CHRIS D. GREEN; Oestrogen regulated mRNAs in the ZR-75-1 human breast cancer cell line. Biochem Soc Trans 1 April 1988; 16 (2): 169–170. doi: https://doi.org/10.1042/bst0160169a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: cDNA, complementary DNA, DHFR, dihydrofolate reductase This content is only available as a PDF. © 1988 Biochemical Society1988 Article PDF first page preview Close Modal You do not currently have access to this content.
Topological control of 3,4-connected frameworks based on the Cu2-paddle-wheel node:tboorpto, and why? (2016)

Two trigonal tritopic ligands with different conformational degree of freedom: conformationally labile H3tcbpa (tris((4-carboxyl)phenylduryl)amine) and conformationally obstructed H3hmbqa (4,4′,4′′-(4,4,8,8,12,12-hexamethyl-8,12-dihydro-4H-benzo[9,1]quino-lizino[3,4,5,6,7-defg]acridine-2,6,10-triyl)tribenzoic acid) are assembled with square-planar paddle-wheel nodes with the aim of selective engineering of the frameworks with tbo and pto underlying net topologies. In the case of H3tcbpa, both topological types were obtained forming non-interpenetrated MOFs namely DUT-63 (tbo) and DUT-64 (pto). Whereas synthesis of DUT-63 proceeds under typical conditions, formation of DUT-64 requires an additional topology directing reagent (topological modifier). Solvothermal treatment of the conformationally hindered H3hmbqa ligand with the Cu-salt results exclusively in DUT-77 material, based on the single pto net. The possibility to insert the salen based metallated pillar ligand into networks with pto topology post-synthetically results in DUT-78 and DUT-79 materials (both ith-d) and opens new horizons for post-synthetic insertion of catalytically active metals within the above-mentioned topological type of frameworks.
The Evolution of Oblique Impact Flow Fields Using Maxwell's Z Model (2003)

Oblique impacts are the norm rather than the exception for impact craters on planetary surfaces. This work focuses on the excavation of experimental oblique impact craters using the NASA Ames Vertical Gun Range (AVGR). Three-dimensional particle image velocimetry (3D PIV) is used to obtain quantitative data on ejection positions, three-dimensional velocities and angles. These data are then used to test the applicability and limitations of Maxwell's Z Model in representing the subsurface evolution of the excavation-stage flow-field center during vertical and oblique impacts.
Improved uniformity in bonded SOI wafers with active layers from 1 to 30 μm at high throughputs (2002)

We present here results of experiments in which we measured and processed bonded SOI wafers with active layers between 1 and 30 /spl mu/m at high throughput using the AcuThin/sup TM/ plasma-assisted chemical etch (PACE) process on our PWS-200 PACE system. The subject wafers were conventionally bonded, ground, and polished. We performed a single PACE process step on the wafers, removing approximately 2 /spl mu/m of silicon while improving the wafer uniformity. For one wafer, the global TTV (GBIR) of the active layer was improved from a starting value of 1.15 /spl mu/m to a final value of 0.14 /spl mu/m and the RMS uniformity of the surface improved from 0.238 to 0.023 /spl mu/m in the single process step. The experiment included off-line wafer measurement using two different metrology systems. For SOI layers less than 7 /spl mu/m, we used an IPEC Precision AcuMap II/sup TM/ to measure the wafers. For SOI layers between 7 and 30 /spl mu/m, we used an IPEC Precision IPM/sup TM/ thickness probe to measure the wafers. Assuming an average removal of 1.5 /spl mu/m on a 150 mm SOI wafer, the throughput of the PWS-200 would be 15 wafers per hour. The PWS-200 PACE system is a promising high-throughput method for producing SOI wafers for bipolar/BiCMOS applications.
Mitochondrial pathology in Parkinson's disease and implications for therapeutic intervention (1999)

Although the ultimate origin and role of mitochondrial pathology in Parkinson's disease (PD) remains controversial, accumulating data suggest that mitochondrial dysfunction is genetic and plays an important role in neurodegeneration. PD mitochondria display abnormal electron transport chain activity, increased reactive oxygen species generation, and abnormal calcium handling. These abnormalities arise, at least in part, from information encoded by mitochondrial DNA. These processes are implicated in current models of neuronal death and validate ongoing efforts to develop mitochondrial-level pharmacologic interventions for the treatment of this disease.<n>Drug Dev. Res. 46:44–50, 1999. © 1999 Wiley-Liss, Inc.
Efficient Computation of Slepian Functions on the Sphere. (2016)

In this work, we develop a new method for the fast and memory-efficient computation of Slepian functions on the sphere. Slepian functions, which arise as the solution of the Slepian concentration problem on the sphere, have desirable properties for applications where measurements are only available within a spatially limited region on the sphere and/or a function is required to be analyzed over the spatially limited region. Slepian functions are currently not easily computed for large band-limits (L > 100) for an arbitrary spatial region due to high computational and large memory storage requirements. For the special case of a polar cap, the symmetry of the region enables the decomposition of the Slepian concentration problem into smaller sub-problems and consequently the efficient computation of Slepian functions for large band-limits. By exploiting the efficient computation of Slepian functions for the polar cap region on the sphere, we develop a formulation, supported by a fast algorithm, for the computation of Slepian functions for an arbitrary spatial region to enable the analysis of modern data-sets that support large band-limits. For the proposed algorithm, we carry out accuracy analysis, computational complexity analysis and review of memory storage requirements. We illustrate, through numerical experiments, that the proposed method enables faster computation, and has smaller storage requirements, while allowing for sufficiently accurate computation of the Slepian functions.
Online facial expression recognition based on personalized galleries (2002)

An online facial expression recognition system based on personalized galleries is presented. This system is built on the framework of the PersonSpotter system, which is able to track and detect the face of a person in a live video sequence. By utilizing the recognition method of Elastic Graph Matching, the most similar person whose images are stored in the gallery can be found, then the personalized gallery of this person is used to recognize the expression on the probe face. A personalized gallery consists of images of the same person showing different facial expressions. Node weighting and weighted voting in addition to Elastic Graph Matching are applied to identify the expression. The performance achieved by this system shows its great potential.
Assembly of CuIn1-xGaxS2 Nanorods into Highly Ordered 2D and 3D Superstructures (2012)

Here, we report self- and directed assembly of CuIn(1-x)Ga(x)S(2) (CIGS) nanorods into highly ordered 2D and 3D superstructures. The assembly protocol is dictated by the ligand environment and is hence chemically tunable. Thiol capped nanorods spontaneously assemble into 3D aligned nanorod clusters over a period of hours with end to end and side to side order. These clusters can be disassembled by ligand exchange with an amine and subsequently reassembled either at a substrate interface or as free floating 2D sheets by directed assembly protocols. This dimensional control of CIGS nanorod assembly, extending over device scale areas with high degrees of order, is highly attractive for applications utilizing these important quaternary photoabsorbers.
Urine is a novel source of autologous mesenchymal stem cells for patients with epidermolysis bullosa (2015)

Thus, USCs provide a novel and non-invasive source of stem cells, which might be applied for gene-therapeutic approaches to improve medical conditions of patients with EB.
What's the Connection? (2008)

A 26-year-old man presented with a 1-month history of persistent cough productive of white sputum, which was occasionally tinged with blood. He reported mild pleuritic chest pain but had no dyspnea, fever, chills, night sweats, or weight loss. The patient had had no epistaxis or episodes of sinusitis. One week before presentation, he received an empirical course of azithromycin, with no resolution of his symptoms.
802.11 denial-of-service attacks: real vulnerabilities and practical solutions (2003)

The convenience of 802.11-based wireless access networks has led to widespread deployment in the consumer, industrial and military sectors. However, this use is predicated on an implicit assumption of confidentiality and availability. While the security flaws in 802.11’s basic confidentially mechanisms have been widely publicized, the threats to network availability are far less widely appreciated. In fact, it has been suggested that 802.11 is highly susceptible to malicious denial-of-service (DoS) attacks targeting its management and media access protocols. This paper provides an experimental analysis of such 802.11-specific attacks – their practicality, their efficacy and potential low-overhead implementation changes to mitigate the underlying vulnerabilities. 1
Faculty Opinions recommendation of Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. (2020)

Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/ refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
3D reconstruction of coronary artery bifurcations from intravascular ultrasound and angiography (2023)

Coronary bifurcation lesions represent a challenging anatomical subset, and the understanding of their 3D anatomy and plaque composition appears to play a key role in devising the optimal stenting strategy. This study proposes a new approach for the 3D reconstruction of coronary bifurcations and plaque materials by combining intravascular ultrasound (IVUS) and angiography. Three patient-specific silicone bifurcation models were 3D reconstructed and compared to micro-computed tomography (µCT) as the gold standard to test the accuracy and reproducibility of the proposed methodology. The clinical feasibility of the method was investigated in three diseased patient-specific bifurcations of varying anatomical complexity. The IVUS-based 3D reconstructed bifurcation models showed high agreement with the µCT reference models, with r<sup>2</sup> values ranging from 0.88 to 0.99. The methodology successfully 3D reconstructed all the patient bifurcations, including plaque materials, in less than 60 min. Our proposed method is a simple, time-efficient, and user-friendly tool for accurate 3D reconstruction of coronary artery bifurcations. It can provide valuable information about bifurcation anatomy and plaque burden in the clinical setting, assisting in bifurcation stent planning and education.
Chromosomal and Extrachromosomal Synthesis of Exfoliative Toxin from Staphylococcus hyicus (2000)

Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless strain P-1 (SHETA) and from plasmid-carrying strains P-10 and P-23 (SHETB) were almost equal. Both of the serotypes of SHET exhibited exfoliation in 1-day-old chickens. The plasmid-cured (P(-)) substrains (P-23C1 and P-23C2) of S. hyicus P-23 did not cause exfoliation in 1-day-old chickens, whereas P(-) substrains (P-10C1 and P-10C2) of strain P-10 caused exfoliation, but they decreased their exfoliative activity. These findings suggest that SHETB was synthesized along with SHETA by strain P-10, whereas the P-23 strain synthesized SHETB alone. The plasmid-carrying strain (P-23) as well as the plasmidless strain (P-1) exhibited the typical clinical signs of exudative epidermitis in pigs. However, plasmid-cured (P(-)) substrains of P-23 (P23C1 and P23C2) did not exhibit the typical clinical signs of exudative epidermitis. These findings suggest that SHETA is synthesized under chromosomal control and SHETB is synthesized under plasmid control and that SHET-producing strains can be divided into three groups: SHETA-producing strains, SHETB-producing strains, and strains producing both toxins.
A new approach for rapid evaluation of the potential field in three dimensions (1999)

Efficient evaluation of the potential field is an essential requirement for simulation of large ensembles of particles in many applications, including astrophysics, plasma physics, molecular dynamics, very large–scale integration systems and micro–electro–mechanical systems. Current methods use multipole expansion of spherical harmonics for the potential field, which is computationally expensive in terms of running time and memory requirements when a high degree of accuracy is desired. In this paper, a new approach is presented for efficient and rapid evaluation of the potential field in three dimensions. The mathematical background for the proposed approach stems from an exponential integral representation of Green's function, 1/r, and an approximation to the integral by using Gauss quadratures, which distinctively differs from the theory of spherical harmonics. The translations are simple in structure, error–free and independent of the approximation, which enables the overall accuracy and computational performance to be controlled externally via the approximation. In addition, the gradient of the potential can be readily retrieved as a by–product of the computational process. More importantly, the memory requirement is independent of the desired degree of accuracy. The technique presented here opens new possibilities for efficient distributed computing and parallel processing of large–scale simulation of particle systems.
A schema for histologic grading of small intestine allograft acute rejection (2003)

This study provides a reliable predictive schema for assessment of the severity of human small bowel acute rejection.
Cloning and periplasmic expression of peptidoglycan-associated lipoprotein (PAL) protein of Legionella pneumophila in Escherichia coli (2010)

Introduction and objective: Legionella pneumophila, the etiological agent of Legionnaires’ disease, is an important cause of both community-acquired and nosocomial pneumonia; therefore, rapid diagnosis and early antibiotic treatment of pneumonia are required. Urinary antigen testing to detect Legionella antigen has proven to be the most powerful diagnostic method. Peptidoglycan-associated lipoprotein (PAL) protein of L. pneumophila, as a component of Legionella antigens, will be detected efficiently by the PAL antigen capture assay and is considered as useful diagnostic antigen to diagnose Legionella infection. Because of the transfer of protein to the periplasmic region of Escherichia coli has numerous advantages including separation from cytoplasmic proteins and the concentration of recombinant proteins in periplasm, the aim of this study was to produce periplasmic PAL protein of L. pneumophila in E. coli. Materials and methods: The pal gene of L. pneumophila serogroup 1 was amplified with specific primers, cloned and expressed under pelB signal sequence and T7 lac promoter in pET26b+ plasmid. Results: The cloning was confirmed with digestion and sequencing of recombinant pET26b-pal plasmid. The expression of r-PAL protein in cytoplasm and periplasmic space of E. coli was approved by SDS-PAGE and western blotting. Conclusion: The results of this study demonstrated that the r-PAL protein successfully expressed in E. coli.
Dual high-stroke and high–work capacity artificial muscles inspired by DNA supercoiling (2021)

Powering miniature robots using actuating materials that mimic skeletal muscle is attractive because conventional mechanical drive systems cannot be readily downsized. However, muscle is not the only mechanically active system in nature, and the thousandfold contraction of eukaryotic DNA into the cell nucleus suggests an alternative mechanism for high-stroke artificial muscles. Our analysis reveals that the compaction of DNA generates a mass-normalized mechanical work output exceeding that of skeletal muscle, and this result inspired the development of composite double-helix fibers that reversibly convert twist to DNA-like plectonemic or solenoidal supercoils by simple swelling and deswelling. Our modeling-optimized twisted fibers give contraction strokes as high as 90% with a maximum gravimetric work 36 times higher than skeletal muscle. We found that our supercoiling coiled fibers simultaneously provide high stroke and high work capacity, which is rare in other artificial muscles.