gebhart/patrepeval huggingface dataset:
Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk (2017)

Blastocystis sp. is a common intestinal parasite infecting humans and a wide range of animals worldwide. It exhibits an extensive genetic diversity and 17 subtypes (STs) have thus far been identified in mammalian and avian hosts. Since several STs are common to humans and animals, it was proposed that a proportion of human infections may result from zoonotic transmission. However, the contribution of each animal source to human infection remains to be clarified. Therefore, the aim of this study was to expand our knowledge of the epidemiology and host specificity of this parasite by performing the largest epidemiological survey ever conducted in animal groups in terms of numbers of species screened. A total of 307 stool samples from 161 mammalian and non-mammalian species in two French zoos were screened by real-time PCR for the presence of Blastocystis sp. Overall, 32.2% of the animal samples and 37.9% of the species tested were shown to be infected with the parasite. A total of 111 animal Blastocystis sp. isolates were subtyped, and 11 of the 17 mammalian and avian STs as well as additional STs previously identified in reptiles and insects were found with a varying prevalence according to animal groups. These data were combined with those obtained from previous surveys to evaluate the potential risk of zoonotic transmission of Blastocystis sp. through the comparison of ST distribution between human and animal hosts. This suggests that non-human primates, artiodactyls and birds may serve as reservoirs for human infection, especially in animal handlers. In contrast, other mammals such as carnivores, and non-mammalian groups including reptiles and insects, do not seem to represent significant sources of Blastocystis sp. infection in humans. In further studies, more intensive sampling and screening of potential new animal hosts will reinforce these statements and expand our understanding of the circulation of Blastocystis sp. in animal and human populations.
Chaperonin 10 as an endothelial‐derived differentiation factor: Role of glycogen synthase kinase‐3 (2009)

The anti-inflammatory peptide early pregnancy factor/chaperonin 10 (cpn10) was identified by 2D-electrophoresis/mass spectrometry as one of the proteins increased in human umbilical cord endothelial cells (HUVEC) after treatment with erythropoietin (EPO). EPO increased the amount of cpn10 released into the medium of HUVEC cultures, despite the absence of a secretory signal peptide. Although immunosupressive agents would represent an indirect advantage for red cell formation under conditions of infection and inflammation, it is possible that cpn10 may have direct effects on erythroid cells. We show that the chaperonin decreased cell proliferation in cultures of the erythroleukemia cell line K562 and increased the amounts of the erythroid differentiation markers glycophorin A and hemoglobin in TF-1 cells. Nevertheless, cpn10 is not a specific erythroid cell differentiation factor, because monolayers of skin fibroblasts overexpressing cpn10 had significantly higher levels of the differentiation marker collagen I than normal fibroblasts. Nothing is known about the mechanism of action of cpn10 in its capacity as a general differentiation factor. An analysis of early changes taking place in K562 cells after incubation with cpn10 using antibody microarrays identified several phosphorylation events, including a decrease of GSK-3alpha phosphorylation. Further studies in TF-1 cells indicated that cpn10 decreased the phosphorylation of cofilin-1 while stimulating that of GSK-3beta. Furthermore, glycophorin A production decreased in the presence of a GSK-3 inhibitor in the same cells. These experiments support the idea that GSK-3-regulated signal transduction pathways are not only important for stem cell maintenance but may be involved in events controlling cell differentiation.
Apramides A−G, Novel Lipopeptides from the Marine CyanobacteriumLyngbya majuscula (2000)

Six new metabolites have been isolated from a lyngbyastatin 2-producing strain of the marine cyanobacterium Lyngbya majuscula collected at Apra Harbor, Guam, and their structures elucidated. These linear lipopeptides have been assigned the trivial names apramides A-F (1-6). From a more recent collection of this cyanobacterium, a structurally related compound, apramide G (7), has been found instead of apramides A-F (1-6). Structure elucidation of the lipopeptides 1-7 is based on spectroscopic techniques and chiral chromatography of hydrolysis products. The apramides appear as NMR-spectroscopically distinguishable conformers in solution, and this has been ascribed to the presence of a thiazole-containing modified amino acid unit.
Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for Detecting Foodborn <i>Salmonella</i> in Raw Milk (2013)

In present study, we reported the performance of a Loop-mediated isothermal amplification (LAMP) assay detecting food-borne pathogen Salmonella. Three pairs of primers were specially designed for recognizing eight distinct sequences on the target invA gene. Time and temperature conditions for amplification of Salmonella were optimized to be 40 min at 61°C. The LAMP assay gave with artificially contaminated raw milk samples detection limit level of 142 CFU/ml which corresponds to 6-9 cells per reaction tube, while the detection level of conventional PCR was 103 CFU/ml. Data on naturally contaminated raw milk samples indicated that the LAMP method was highly specific and sensitive, giving 89.58% concordance with the ISO 6579 reference method for the samples without enrichment and 100% concordance for the samples after enrichment.
Protein damage and methylation-mediated repair in the erythrocyte (1995)

Research Article| March 01 1995 Protein damage and methylation-mediated repair in the erythrocyte P Galletti; P Galletti *Institute of Biochemistry of Macromolecules, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy Search for other works by this author on: This Site PubMed Google Scholar D Ingrosso; D Ingrosso *Institute of Biochemistry of Macromolecules, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy Search for other works by this author on: This Site PubMed Google Scholar C Manna; C Manna *Institute of Biochemistry of Macromolecules, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy Search for other works by this author on: This Site PubMed Google Scholar G Clemente; G Clemente †Institute of Food Science and Technology, C.N.R., Avellino, Italy Search for other works by this author on: This Site PubMed Google Scholar V Zappia V Zappia *Institute of Biochemistry of Macromolecules, Medical School, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1995) 306 (2): 313–325. https://doi.org/10.1042/bj3060313 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation P Galletti, D Ingrosso, C Manna, G Clemente, V Zappia; Protein damage and methylation-mediated repair in the erythrocyte. Biochem J 1 March 1995; 306 (2): 313–325. doi: https://doi.org/10.1042/bj3060313 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1995 The Biochemical Society, London1995 Article PDF first page preview Close Modal You do not currently have access to this content.
Ultra-fine Pulverization of Rice: Effects on Hydration Properties and Enzymatic Hydrolysis (2013)

We studied the hydration properties and enzymatic hydrolysis of micro-sized rice flour obtained by dry jet-pulverization to find new applications for the flour in the food industry. Ultra-fine rice flour (both white and brown) with a mean size of <5 μm was far more dispersible than coarser flours (15–120 μm). Dispersibility increased with finer mean particle size and higher starch damage. The ultra-fine flour also had the highest solubility, swelling power, water absorption index, and glucose release. Dry jet-pulverization to <5 μm mean size and consequent starch damage to (>30%) decreased enthalpy and gave very different hydration properties and enzymatic hydrolysis than in coarser flours.
The Development of Synthetic Routes to 1,1,n,n‐Tetramethyl[n](2,11)teropyrenophanes (2019)

A concise synthetic approach to 1,1, n , n ‐tetramethyl[ n ](2,11)teropyrenophanes has been developed. It involves the construction of triply‐bridged pyrenophanes, during which the three bridges are installed successively using Friedel‐Crafts alkylation, Wurtz coupling and McMurry reactions. At the same time, the innate regiochemical preferences of pyrene toward electrophilic aromatic substitution are relied upon to control the substitution pattern. A cyclodehydrogenation reaction is then employed to generate the teropyrene system directly in a nonplanar conformation. The crystal structure of 1,1,7,7‐tetramethyl[7](2,11)teropyrenophane was determined and the teropyrene system was found to have an end‐to‐end bend angle of 177.9°.
Pimaradiene Diterpenes from Acanthopanax koreanum (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPimaradiene Diterpenes from Acanthopanax koreanumYoung H. Kim, Bo S. Chung, and Ushio SankawaCite this: J. Nat. Prod. 1988, 51, 6, 1080–1083Publication Date (Print):November 1, 1988Publication History Published online1 July 2004Published inissue 1 November 1988https://pubs.acs.org/doi/10.1021/np50060a005https://doi.org/10.1021/np50060a005research-articleACS PublicationsRequest reuse permissionsArticle Views322Altmetric-Citations58LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Non-reciprocal gyrotropy in graphene: New phenomena and applications (2012)

The non-reciprocal gyrotropic properties of magnetically biased graphene are presented and subsequent potential applications are proposed. Graphene exhibits strong Faraday rotation at microwave frequencies. The amount of rotation can be controlled by both an applied static magnetic field, which controls the cyclotron resonance frequency, and an applied static electric field, which tunes the chemical potential of the material. As an application of Faraday rotation, we present, beyond recently reported non-reciprocal components, a waveguide setup for the extraction of the conductivity tensor of graphene. Moreover, it is shown that a graphene strip supports surface magneto-plasmon modes, each of which propagates along one of its edges, at THz frequencies. Exploiting this effect, a non-reciprocal phase shifter can be realized by placing a conducting plate along one side of the strip.
Mother-to-infant transmission of human immunodeficiency virus type 1 involving five envelope sequence subtypes (1997)

Genetic analysis of human immunodeficiency virus type 1 (HIV-1) from cases of mother-to-infant transmission were analyzed in an effort to provide insights into the viral selection that may occur during transmission, as well as the timing and source of transmitted viruses. HIV-1 env genes obtained from seven mothers and their perinatally infected infants in Sweden were studied. Five envelope sequence clades (A to E) were found to be represented. We used a heteroduplex tracking assay (HTA) to assess the genetic relatedness between early viral isolates from the infants and serial maternal virus populations taken during pregnancy and at delivery. HTA findings were used to select for DNA sequence analysis maternal virus populations that were either closely or more distantly related to the infant virus. In each case, nucleotide sequence analysis confirmed the genetic relationships inferred by the HTA. Only maternal peripheral blood was sampled, and large sets of maternal specimens throughout pregnancy were generally not available. However, no consistent correlation was found to support the hypothesis that infant viruses should match blood-derived maternal virus genotypes found early in pregnancy if infants were found to be infected at birth or, conversely, that infant viruses should match blood-derived maternal virus genotypes found at delivery if infants were found to be infected only some time later.
Patch-Based Occlusion Culling for Hardware Tessellation (2012)

We present an occlusion culling algorithm that leverages the unique characteristics of patch prim- itives within the hardware tessellation pipeline. That is, unseen patches are costly to compute, but easy to nd screen space bounds for. Our algorithm uses the well known hierarchical Z-buer approach, but we present a novel variant that uses temporal coherence to maintain lists of visible and occluded patches. Patches may be an- imated and have applied displacement maps. We also allow traditional triangle mesh geometry to serve as oc- cluders. This makes our scheme ideally suited for patch based articulated character models, moving within a polygonal environment.
Solid-phase synthesis of peptides with C-terminal asparagine or glutamine. An effective, mild procedure based on N alpha-fluorenylmethyloxycarbonyl (Fmoc) protection and side-chain anchoring to a tris(alkoxy)benzylamide (PAL) handle. (1990)

Attempts to anchor Fmoc-asparagine or glutamine as p-alkoxybenzyl esters for solid-phase peptide synthesis are fraught with difficulties. A convenient and effective method to prepare peptides with C-terminal asparagine or glutamine involves quantitative attachment of N alpha-Fmoc-C alpha-tert.-butyl aspartate or glutamate via the free omega-carboxyl groups to a tris(alkoxy)benzylamino (PAL) support. Chain elongation proceeds normally by standard Fmoc chemistry, and treatment with acid, e.g., CF3COOH--CH2Cl2, 90 min at 25 degrees, releases the desired peptides in greater than 95% yields without side reactions at the C-terminus. Feasibility of the approach has been demonstrated by the syntheses of the C-terminal octapeptide from human proinsulin, H-Leu-Ala-Leu-Glu-Gly-Ser-Leu-Gln-OH, and the serum thymic factor pGlu-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn-OH.
Modeling and Evaluation of Pore Clogging of Membrane in Membrane Filtration (2009)

用水や廃水処理に膜濾過を適用する際の最大の問題点は膜細孔の閉塞やケーク形成による膜ファウリングである．本稿では，精密濾過や限外濾過などのコロイド溶液を対象とした膜濾過における膜細孔の閉塞に特に焦点を絞り，膜ファウリングを支配する基本的なメカニズムを概説する．Kozeny–Carman式に立脚し，深層濾過の進行とともに膜の空隙率と比表面積が変化すると考えることにより，膜細孔の閉塞の解析に最も広く用いられている閉塞濾過法則の一般化特性式が理論的に導かれた．最近では，膜細孔の閉塞と濾過ケークの成長が連続して，または同時に生じる複雑な濾過挙動を記述するいくつかの新しい複合モデルが報告されている．特に，水処理における効率的な膜濾過技術を確立するために，単なる物理逆洗や他の周期ダイナミック操作では充分に回復しない膜細孔の非可逆的な閉塞挙動の適切なモデル化が，将来重要となることが予測される．
Thermoresponsive, stretchable, biodegradable and biocompatible poly(glycerol sebacate)-based polyurethane hydrogels (2015)

This work reports the synthesis and characterization of thermoresponsive, stretchable, biodegradable and biocompatible poly(glycerol sebacate)-based polyurethane hydrogels.
Probiotics and Postprandial-Eating Associated Symptoms (2009)

Purpose: Recently, there has been growing interest in the use of probiotics to ameliorate various digestive symptoms. The majority of studies in this field have focused on the effect of probiotics on lower gastrointestinal (GI) symptoms. Upper GI and postprandial symptoms are common functional GI complaints, and possess a significant impact on patient well-being and quality of life. However, there is no data on the effect of probiotics on upper GI, and specifically on postprandial GI symptoms. Aim: To investigate the effect of probiotics on common postprandial symptoms in subjects with functional GI disorders using two probiotic products previously shown to be beneficial in alleviating lower functional GI symptoms. Methods: Subjects who participated in two separate probiotic clinical trials were evaluated for the effect of the intervention on their postprandial and eating associated symptoms. The intervention included 5x109 cfu total Bifidobacterium animalis lactis Bb12 (BB12) and 1.0g inulin per day for six weeks (Study 1) or Lactobacillus acidophilus (L-NCFM) and Bifidobacterium lactis Bi-07 1x1011 cfu total per day for eight weeks (Study 2). Postprandial symptoms were assessed using a specific questionnaire designed to capture information about the frequency, characteristics, and severity of postprandial symptoms. The severity of each symptom was assessed on a 5-point likart scale. Pre- to mid- and pre- to post-intervention changes in frequency and severity of postprandial symptoms for each probiotic intervention vs. placebo were assessed using a two-sided t test with a significance level of p<0.05. Results: A total of 117 (probiotic n=30, placebo n=30 in Study 1 and probiotic n=30, placebo n=27 in Study 2) patients were enrolled. Study population consisted of 72% females, 84% whites, and a mean age of 37 years. Baseline demographics were similar between the two groups in both studies. No significant pre- to -post changes in the frequency or severity of symptoms were noted in either probiotic intervention compared to placebo, despite significant clinical benefits on lower GI symptoms (overall well being in study 1 and bloating in study 2); and an observed beneficial effect on GI physiology (reduced colonic transit time) in Study 1. Conclusion: Although negative, these results are important in directing clinicians and patients in regard to expected beneficial effects of these probiotic interventions. Our study results further emphasize that the effect of probiotics is product and symptom specific. Disclosure: Dr Ringel- Grant/Research Support from Danisco and General Mills, Inc. This research was supported by an industry grant from Danisco; General Mills Inc.
Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection (2018)

Abstract Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and for isolation of specific cell populations for study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However, human PSCs are known to have a low frequency of gene knock-in (KI) by HDR, making reporter line generation an arduous process. Here, we report a methodology for scarless KI of large fluorescent reporter genes into PSCs by transient selection with puromycin or zeocin. With this method, we can perform targeted KI of a single reporter gene with up to 65% efficiency, as well as simultaneous KI of two reporter genes into different loci with up to 11% efficiency. Additionally, we demonstrate that this method also works in mouse PSCs.
Depolarization effect in rare‐earth doped Y2O3 films in blue and UV spectral range (2015)

Abstract The 200 to 300 nm thick, Er and Er,Yb doped Y 2 O 3 films deposited onto silicon substrate by spin coating have been studied by spectroscopic ellipsometry over the 192‐1680 nm spectral range at room temperature. All samples have been found to be strongly depolarizing in the blue and UV part of the spectrum. Complimentary examination of the sample surfaces, using confocal photoluminescence microscopy has disclosed the non‐uniform distribution of the rare‐earth dopants. The depolarization effects have then been modeled and found to be best reproduced by taking the thickness non‐uniformity as the main source of depolarization. The optical constants of the studied films have been determined after four‐step modeling with sequential decrease of the mean square error. (© 2015 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Corrosion of Carbon Steel and Cr-containing Alloy Steels in CCS Systems (2019)

Abstract For transporting large amounts of CO2 (compressing to supercritical or liquid state) over long distance during Carbon Capture and Storage process, the most logical and economical option is pipelines network made from carbon steels such as X65 and X70 steels, which, however, is susceptible to corrosion in the supercritical CO2 systems containing free water. This work aims to evaluate the performance of the newly developed Cr-containing steels, which fills the gap between carbon steels and anticorrosion alloys in view of the cost and corrosion resistance, in the static and dynamic water-saturated supercritical CO2 environments. Results showed that X70 and 3Cr steels mainly suffered from localized corrosion, and both the general and localized corrosion rates under static condition were higher than that under dynamic condition. 3 wt.% Cr content was unable to effectively enhance the corrosion resistance of the steel. However, 6.5Cr steel presented superior localized corrosion resistance in supercritical CO2 environments, and could be selected as a replacement for carbon steel under these conditions.
Self-Diffusion of Ions in Molten Electrolyte under the Influence of an Electric Field (1955)

By taking into account both self-diffusion and electric migration of the radioactive ions formed by the electrolytic dissolution of a radioactive metal anode, an equation has been derived for the distribution of the radioactivity as a function of the distance from the anode surface along the diffusion path. Based on this equation, the error involved in the direct determination of ionic mobility by measuring the distance of spreading of the radioactivity and neglecting the role of self-diffusion is analyzed. It is shown that for a system with a given diffusivity, the error decreases with the increase of the time of electrolysis, t, and the applied potential, E. To obtain the true ionic mobility, it is suggested that measurements should be made for a number of ``t'' or ``E'' values. The apparent ionic mobility obtained is then plotted against 1/t or 1/E and extrapolated to 1/t=0 or 1/E=0 to give the true value.
An active strain sensing system based on single‐longitudinal‐mode distributed Bragg reflector fiber laser and its applications in the measurement of erbium‐doped fiber birefringence (2011)

Abstract An active strain sensing system based on single‐longitudinal‐mode distributed Bragg reflector fiber laser is proposed and experimentally demonstrated. Owing to the short laser cavity length and birefringence‐induced mode splitting, the proposed laser in single‐longitudinal‐mode operation provides a beat signal from 1.598 to 2.53 GHz as the strain applied onto the fiber laser changes, and through beat frequency interrogation the proposed laser sensor turns out a strain sensitivity of ∼0.21 MHz/με. Moreover, by simultaneously monitoring the laser wavelength and beat frequency, strain dependence of erbium‐doped fiber birefringence has also been measured, and experimental results show that erbium‐doped fiber birefringence has a strain sensitivity of ∼1.59 × 10 −9 /με. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 53:2508‐2512, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26302