gebhart/patrepeval huggingface dataset:
Involvement of Focal Adhesion Kinase in Inhibition of Motility of Human Breast Cancer Cells by Sphingosine 1-Phosphate (1999)

Sphingosine 1-phosphate (SPP), a bioactive sphingolipid metabolite, inhibits chemoinvasiveness of the aggressive, estrogen-independent MDA-MB-231 human breast cancer cell line. As in many other cell types, SPP stimulated proliferation of MDA-MB-231 cells, albeit to a lesser extent. Treatment of MDA-MB-231 cells with SPP had no significant effect on their adhesiveness to Matrigel, and only high concentrations of SPP partially inhibited matrix metalloproteinase-2 activation induced by Con A. However, SPP at a concentration that strongly inhibited invasiveness also markedly reduced chemotactic motility. To investigate the molecular mechanisms by which SPP interferes with cell motility, we examined tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin, which are important for organization of focal adhesions and cell motility. SPP rapidly increased tyrosine phosphorylation of FAK and paxillin and of the paxillin-associated protein Crk. Overexpression of FAK and kinase-defective FAK in MDA-MB-231 cells resulted in a slight increase in motility without affecting the inhibitory effect of SPP, whereas expression of FAK with a mutation of the major autophosphorylation site (F397) abolished the inhibitory effect of SPP on cell motility. In contrast, the phosphoinositide 3'-kinase inhibitor, wortmannin, inhibited chemotactic motility in both vector and FAK-F397-transfected cells. Our results suggest that autophosphorylation of FAK on Y397 may play an important role in SPP signaling leading to decreased cell motility.
Discourse Planning with an N-gram Model of Relations (2015)

While it has been established that transitions between discourse relations are important for coherence, such information has not so far been used to aid in language generation. We introduce an approach to discourse planning for conceptto-text generation systems which simultaneously determines the order of messages and the discourse relations between them. This approach makes it straightforward to use statistical transition models, such as n-gram models of discourse relations learned from an annotated corpus. We show that using such a model significantly improves the quality of the generated text as judged by humans.
Structural Characterization of Molybdenum(V) Species in Aqueous HCl Solutions (2007)

Mo(V) aqua-chloro complexes in hydrochloric acid solutions have been studied by means of Mo K- and L2,3-edge X-ray absorption and Raman spectroscopic methods. The solid compounds (HPPh3)2[MoOCl5] (1), 6[MoOCl4(H2O)]-.10(pyH)+.4Cl- (2), and (pyH)2[Mo2O4Cl4(trans-OH2)2] (3) were used for structural comparisons. The compound 2 crystallizes in the orthorhombic space group Pmma (no. 51) with a=21.398(3), b=8.057(4), c=13.330(4) A, and Z=4. In 0.2 M solutions of MoCl5 in 7.4-9.4 M HCl the mononuclear [MoOCl4(OH2)]- complex dominates with the bond distances Mo=O 1.66(2) A, Mo-Cl 2.38(2) A, and Mo-OH2 2.30(2) A. Its Raman band at 994 cm-1 for the Mo=O symmetric stretching vibration is closer to that of 2 (988 cm-1) than of 1 (969 cm-1). The Mo K-edge EXAFS spectrum for 0.2 M MoCl5 in 1.7 M HCl solution reveals a dinuclear [Mo2O4Cl6-n(OH2)n]n-4 (n=2, 3) complex with a double oxygen bridge and the average distances Mo=O 1.67(2) A, Mo-(mu-O) 1.93(2) A, Mo-Cl 2.47(3) A, Mo-Mo 2.56(2) A, and a short Mo-OH2 distance of 2.15(2) A, which implies that at least one of the aqua ligands is in equatorial position relative to the two axial Mo=O bonds. This position differs from the Mo-OH2 configuration exclusively trans to the M=O groups of the isomeric (with n=2) dinuclear complex in 3. The difference in the ligand field is also reflected in their L2,3-edge XANES spectra. For 0.2 M MoCl5 solutions in intermediate HCl concentrations (3.7-6.3 M) the Raman bands at 802 cm-1 (Mo-O-Mo) and 738 cm-1 (Mo-(mu-O)2-Mo) verify three coexisting classes of Mo(V) complexes: mononuclear complexes together with dinuclear mono-oxo (e.g., [Mo2O3Cl6(H2O)2]2-) and dioxo bridged species, even though principal component analysis (PCA) of the corresponding series of EXAFS spectra only could distinguish two major components. By fitting linear combinations of the appropriate EXAFS oscillation components, dioxo-bridged dinuclear complexes were found to dominate at HCl concentrations<or=4.5 M, a conclusion supported by the Mo L2,3-edge XANES spectra.
Tetrathiafulvalene, Polymer-supported (2003)

(reagent used in the radical-polar crossover reaction1) Alternate Name: PS-TTF. Analysis of Reagent Purity: combustion analysis. Preparative Methods: PS-tetrathiafulvalene 3 can be prepared by the reaction of alcohol 12 with sodium hydride in dimethylacetamide as solvent with two different polymer types–-a macroporous resin [derived from 25% chloromethylstyrene, 65% divinylbenzene, and 10% styrene with toluene as the porogen] and a gel-type resin [derived from 25% chloromethylstyrene, 2% divinylbenzene, and 73% styrene] prepared using suspension polymerization methodology3 (eq 1). (1) Purification: can be purified by filtration, washing, and drying.
Realizing the potential of blockchain technologies in genomics (2018)

Genomics data introduce a substantial computational burden as well as data privacy and ownership issues. Data sets generated by high-throughput sequencing platforms require immense amounts of computational resources to align to reference genomes and to call and annotate genomic variants. This problem is even more pronounced if reanalysis is needed for new versions of reference genomes, which may impose high loads to existing computational infrastructures. Additionally, after the compute-intensive analyses are completed, the results are either kept in centralized repositories with access control, or distributed among stakeholders using standard file transfer protocols. This imposes two main problems: (1) Centralized servers become gatekeepers of the data, essentially acting as an unnecessary mediator between the actual data owners and data users; and (2) servers may create single points of failure both in terms of service availability and data privacy. Therefore, there is a need for secure and decentralized platforms for data distribution with user-level data governance. A new technology, blockchain, may help ameliorate some of these problems. In broad terms, the blockchain technology enables decentralized, immutable, incorruptible public ledgers. In this Perspective, we aim to introduce current developments toward using blockchain to address several problems in omics, and to provide an outlook of possible future implications of the blockchain technology to life sciences.
Evidence for a relation between metabolic derangements and increased levels of inflammatory mediators in a subgroup of patients with chronic obstructive pulmonary disease. (1996)

A subset of patients with COPD with an increased REE and decreased FFM have increased levels of acute phase reactant proteins and inflammatory cytokines in their serum; these phenomena may be causally related.
Parámetros de normalidad con Microscopia Especular del endotelio corneal en pacientes de 12 a 70 años del Servicio de Oftalmología del Hospital Central de San Cristóbal (2015)

Objetivo: Determinar la densidad y morfologia de las celulas del endotelio corneal de pacientes de 12 a 70 anos de edad mediante el uso del Microscopio Especular de No Contacto CSO® durante el periodo de tiempo de Marzo a Julio del 2012. Metodo: Investigacion del tipo transversal, descriptiva y prospectiva. La poblacion estuvo compuesta por pacientes de edades comprendidas entre los 12 a 70 anos, que no presenten patologia corneal, ocular y/o sistemica asociada entre los meses de Marzo a Julio de 2012, cumpliendo con los criterios de distribucion normal. Resultados: Se organizaron los datos obtenidos en una tabla de Microsoft Office Excel 2007, para realizar un analisis posterior mediante el uso del SPSS version 18. La data fue analizada mediante estadistica descriptiva, estableciendo la media y la desviacion tipica de cada uno de los parametros, fueron analizados mediante pruebas estadisticas inferenciales del tipo ANOVA y prueba post hoc de Duncan. Conclusiones: Dos de los tres parametros analizados presentaron valores considerados como normales: densidad endotelial y hexagonalidad. A diferencia de otros estudios clinicos se puede evidenciar que los pacientes seleccionados para este estudio no cumplen con los patrones de normalidad para el coeficiente de variacion establecido en 0,25 en otras investigaciones; no existen diferencias significativas en relacion con la condicion socioeconomica para todas las variables consideradas. Existe una relacion inversa entre la edad y la densidad endotelial, a mayor edad menor densidad endotelial.
Morphology and Viscoelasticity of Actin Networks Formed with the Mutually Interacting Crosslinkers: Palladin and Alpha-actinin (2021)

Actin filaments and associated actin binding proteins play an essential role in governing the mechanical properties of eukaryotic cells. Even though cells have multiple actin binding proteins (ABPs) that exist simultaneously to maintain the structural and mechanical integrity of the cellular cytoskeleton, how these proteins work together to determine the properties of actin networks is not clearly understood. The ABP, palladin, is essential for the maintenance of cell morphology and the regulation of cell movement. Palladin coexists with -actinin in stress fibers and focal adhesions and binds to both actin and -actinin. To obtain insight into how mutually interacting actin crosslinking proteins modulate the properties of actin networks, we characterized the micro-structure and mechanics of actin networks crosslinked with palladin and -actinin. We first showed that palladin crosslinks actin filaments into bundled networks which are viscoelastic in nature. Our studies also showed that composite networks of -actinin/palladin/actin behave very similar to pure palladin or pure -actinin networks. However, we found evidence that palladin and -actinin synergistically modify network viscoelasticity. To our knowledge, this is the first quantitative characterization of the physical properties of actin networks crosslinked with two mutually interacting crosslinkers.
Immunohistochemical localization of ANG II AT1 receptor in adult rat kidney using a monoclonal antibody (1997)

Molecular and functional studies have suggested that AT1 receptors are present in most nephron segments, yet direct demonstration of AT1 at these sites is lacking. The present study was performed to determine the intrarenal localization of the AT1 receptor utilizing a monoclonal anti-peptide (amino acid residues 8-17) antibody (6313/G2) in adult male Sprague-Dawley rats. Western blot analysis of kidney protein extracts showed a predominant 41-kDa immunoreactive band corresponding to the molecular weight of the deduced cDNA sequence. To determine optimal fixation conditions, kidney tissues were immersion fixed in Bouin's solution, 10% buffered Formalin, or 4% paraformaldehyde. Specificity of immunostaining was documented by preadsorption of the antibody with the immunogenic peptide sequence. Prominent AT1 immunostaining was visualized in the proximal tubule brush-border and basolateral membranes. In addition, distal tubules, cortical and medullary collecting ducts, and the renal arterial vasculature exhibited specific immunoreactivity. Glomerular staining for AT1 was observed in mesangial cells and podocytes. Macula densa cells stained positively. Similar localization of the AT1 receptor was obtained using the three tissue fixation methods, although the intensity of vascular and glomerular staining was highest in Bouin-fixed tissues. The present study demonstrates that the AT1 receptor is more widely distributed along the nephron than previously described and includes renal vascular smooth muscle and proximal and distal epithelial sites.
Reviews (1966)

Reviews WINCHESTER ORDINATIONS 1660–1829 , Vol. II, compiled by Arthur J. Willis ; pp. 148 ; 50s. A HAMPSHIRE MISCELLANY III , compiled by Arthur J. Willis ; pp. 94 ; 21s. Both published by the author, Hambledon , Lyminge, Folkestone, Kent, 1965 . M. H. PORT M. H. PORT Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume 13, Issue 2, February 1966, Pages 73–74, https://doi.org/10.1093/nq/13-2-73 Published: 01 February 1966
Prediction of Distortion Produced on Welded Structures during Assembly Using Inherent Deformation and Interface Element (2009)

The objective of this research is to develop a practically useful finite element method for predicting distortions of structures under welding assembly. In this method, distortions produced by various thermal processes, such as the cutting, the forming, the welding and the straightening can be considered. These distortions are simulated using the inherent strain. On the other hand, the detail of how the gaps and the misalignments are controlled during the fitting process also has significant influence on the distortion. The gaps and the misalignments between parts are modeled using the interface element. The potential capability of the proposed method is demonstrated through simple examples.
15 K pulse tube design for ECHO mission (2014)

The Exoplanet Characterisation Observatory (EChO) is a proposed space telescope designed to characterize the atmospheres of nearby transiting exoplanets. Its detectors will operate in the 0.4 to 11 micromillimeter range. Two kinds of detectors are currently able to provide the desired sensitivity in this range. Depending on the technology used, cooling to either 6 K or about 30 K will be required. For the former solution, a JT cooler coupled to a pulse tube cooler could be used whereas for the latter, a pulse tube cooler would provide the cooling power. Pulse tube coolers are particularly well adapted for the cryogenics for such mission because of the low level of vibration required and of the temperature range. We developed multistage pulse tube coolers able to cool down to temperature as low as 6 K, with efficient operation from 10 K to 40 K. A design based on our tested prototypes is proposed to fulfill the need for the ECHO missions. This paper describes the experimental results measured with demonstrator models. In particular measured performances of efficient cooling power at 10 K are presented. Several possible configurations for the ECHO cooler will be discussed as well.
Matrix Metalloproteinases and Their Inhibitors in Human Pituitary Tumors (1999)

No correlation could be established between the invasive potential of tumors and MMP-1, -2, and -3 expression levels. Some correlation was observed between MMP activity detected by zymography and tumor grade. A good inverse correlation was observed between TIMP-2 and TIMP-3 expression levels and tumor grade. These data suggest that monitoring the expression of TIMP-2 and TIMP-3 or gelatinolytic activity could be of prognostic value.
Histamine Induces Tyrosine Phosphorylation of Endothelial Cell-to-Cell Adherens Junctions (1999)

Endothelial adherens junctions (AJ) promote intercellular adhesion and may contribute to the control of vascular permeability. These structures are formed by a transmembrane and cell-specific adhesive protein, vascular endothelial (VE)-cadherin, which is linked by its cytoplasmic tail to intracellular proteins called catenins (alpha-catenin, beta-catenin, and plakoglobin) and to the actin cytoskeleton. Little is known about the functional regulation of AJ in endothelial cells. In this study, we analyzed the effect of histamine on AJ organization in cultured endothelial cells. We first observed that histamine induced detectable intercellular gaps only in loosely-confluent cells, whereas this effect was strongly reduced or absent in long-confluent cultures. Despite this difference, in vitro permeability was augmented by histamine in both conditions. In resting conditions, tyrosine phosphorylation of AJ components and permeability values were higher in recently-confluent cells as compared with long-confluent cells. Histamine did not affect the phosphorylation state of AJ in recently-confluent cells but strongly increased this parameter in long-confluent cultures. In addition, in long-confluent cells, histamine caused dissociation of VE-cadherin from the actin cytoskeleton measured by a decrease of the amount of the molecule in the detergent-insoluble fraction of the cell extracts. Dibutyryl cAMP was able to prevent the effect of histamine on both tyrosine phosphorylation of AJ components and on endothelial permeability. The effect of histamine was specific for VE-cadherin because the phosphorylation state of neural (N)-cadherin, the other major endothelial cadherin, was unchanged by this agent. Hence AJ components are a target of histamine activation cascade; we suggest that induction of tyrosine phosphorylation of VE-cadherin and catenins contributes to the histamine effect on permeability, even in absence of frank intercellular gaps and cell retraction.
Modulation of inflammation and oxidative stress in canine chondrocytes (2013)

Canine chondrocytes responded to the oxidative stress caused by exposure to hydrogen peroxide and cytokines. Exposure to oxidative stress inducers could result in perturbation of chondrocyte and cartilage homeostasis and could contribute to the pathophysiology of osteoarthritis. Use of antioxidants, on the other hand, may be helpful in the treatment of arthritic dogs.
Chemické složení loštických pohárů (2008)

The paper summarizes results of geochemical examination of the Lostice pottery. A clay nodule found during archaeological works was used for a part of tumbler replicas´ production as a plastic raw material. At the temperature over 1 200 °C, the replicas made of loess collapsed. The affect of garnets on formation of “blebs” was experimentally verified. The results showed that the ceramic tumblers of the (010) and (020) groups were made of clay soil with the addition of almandine garnet as non-plastics. The “blebs” form by thermal decomposition of almandine. The firing temperature must exceed 1 200 °C. Cordierite (sekaninaite, possibly ossumilite) crystallizes from the melt. Another part of replicas was made of local loess.
Cellular Fibronectin Expression in Human Trabecular Meshwork and Induction by Transforming Growth Factor-β2 (2013)

This study demonstrated that cFN isoforms are expressed and induced in HTM cells by TGF-β2. Also, increased EDA isoform protein levels were seen in GTM tissues. Our findings suggest that induction of cFN isoform expression in the TM ECM may be a novel pathologic mechanism involved in the TM changes associated with glaucoma.
Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10). (1982)

A monoclonal T-cell-derived suppressor factor specific for the terpolymer poly(LGlu60LAla30LTyr10) produced by the T-cell hybridoma 258 C4.4, was purified to homogeneity. This was accomplished by fractionation of the culture medium by using a combination of affinity chromatography and reverse-phase and ion-exchange high-performance liquid chromatography. The purified factor is composed of a single Mr 24,000 polypeptide chain, and the homogeneous protein maintains the ability to suppress antibody and T-cell proliferative responses to poly(LGlu60LAla30LTyr10) specifically. The specific activity of pure suppressor factor is calculated to be 8 X 10(7) units/micrograms.
Distribution of actuators in vibration control of adaptive structures (2005)

Considers the placement of distributed actuators in motion control of flexible structures. By representing the system dynamics in terms of natural modes of vibration, simple conditions are developed from which degrees of controllability of individual modes for a given actuator distribution are determined. Systematic procedures for optimal placement of distributed actuators are proposed, which are extensions of the methods developed earlier for point actuators. The proposed optimality criteria for actuator distribution seek to minimise the actuator input energy under a variety of operating conditions. Minimal levels of controllability and observability of each controlled mode are. Achieved with this approach. The method is illustrated for a simply supported beam and a rectangular plate controlled by distributed actuators of various shapes.
Transient T cell accumulation in lymph nodes and sustained lymphopenia in mice treated with FTY720 (2005)

FTY720 (2-amino-2-[2-(4-octylphenyl)ethyl]propane-1,3-diol hydrochloride) is an orally available immunomodulatory agent that induces severe peripheral blood lymphopenia. Most studies of these lymphopenic effects have been limited to short-term exposure to FTY720. FTY720 alters the ability of lymphocytes to respond to sphingosine-1-phosphate (S1P) through S1P receptors, particularly S1P1. FTY720 affects different leukocyte populations and their trafficking through major lymphoid organs. We show the dynamics of CD4 T, CD8 T, and B lymphocyte recirculation in all major lymphoid compartments during 21-day FTY720 treatment of normal C57BL/6 mice. Following a transient increase in peripheral lymph nodes and Peyer's patches, lymphocyte recirculation reaches a new steady state. Other lymphoid organs show transient changes in lymphocyte composition with various patterns. At 21 days of FTY720 treatment, total body lymphocyte content is reduced by 20% and blood lymphocytes by 80%. Modeling suggests that the new steady state is due to a combination of reduced naive lymphocyte release from the thymus and a transient reduction of lymphocyte egress from lymph nodes. Our data indicate that the commonly held belief that FTY720 blocks lymphocyte egress from lymph nodes cannot fully explain the lymphocyte dynamics observed with prolonged treatment.
Radar spectrum opportunities for cognitive communications transmission (2008)

In relation to opportunistic access to radar spectrum, the impact of the radar on a communication system is investigated in this paper. This paper illustrates that by exploring the spatial and temporal opportunities in the radar spectrum and therefore improving the tolerance level to radar interference, a substantial increase on the throughput of a communication system is possible. Results are presented regarding the impact of swept radars on a WiMAX system. The results show the impact of SIR (Received WiMAX signal to received radar signal ratio), radar antenna radiation patterns and rotation period estimation on the feasibility of radar spectrum access.
Comparison of rule-based and artificial neural network approaches for improving the automated detection of clustered microcalcifications in mammograms (1995)

Forty-six thousnad women die each year in the US from breast cancer. Mammography is the best method of detecting breast cancer and has been shown to reduce breast cancer mortality in randomized controlled studies. Clustered microcalcifications are often the first sign of breast cancer in a mammogram. The use of a second reader may improve the sensitivity of detecting clustered microcalcifications. Our laboratory has developed a computerized scheme for the detection of clustered microcalcifications that is undergoing clinical evalution. This paper concerns the feature analysis stage of the computerized scheme, which is designed to remove false-computer detections. We have examined three methods of feature analysis: rule-based (the method currently used in the clinical system), an artificial neural network (ANN), and a combined method. To compare the three methods, the false-positive (FP) rate at a sensitivity of 85% was measured on two separate databases. The average number of FPs per image were: 0.54 for rule-based, 0.44 for ANN, and 0.31 for the combined method. The combined method had the highest performance and will be incorporated into the clinical system.
DESIGN OF THE SILENT CEREBRAL INFARCT TRANSFUSION (SIT) TRIAL (2010)

The SIT Trial could lead to a change in standard care practices for children affected with SCA and SCI, with a consequent reduction in neurological morbidity.