gebhart/patrepeval huggingface dataset:
Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis (2010)

A simple and rapid DNA extraction protocol capable of obtaining high-quality and quantity DNA from a large number of individuals is essential for assaying population and phylogenetic studies of plant pathogens. Most DNA extraction protocols used with oomycetes are relatively lengthy and cumbersome for high throughput analysis. Commercial kits are widely used, but low quantities of DNA are usually obtained, and with large scale analysis multiple isolations are required. A protocol for DNA isolation from Phytophthora and Pythium suitable for the evaluation of a large set of molecular markers was modified from one previously developed for soybean seed. There was a one to three fold increase in the amount of DNA that was extracted using the modified protocol compared to a commercial kit. The DNA obtained using the modified protocol was suitable for the amplification of microsatellite markers as well as the ITS region. This protocol is inexpensive, easy, quick, and efficient in terms of the volume of reagents and the number of steps involved in the procedure. The method may be applicable to other oomycetes and effectively implemented in other laboratories.
A visibility matching tone reproduction operator for high dynamic range scenes (1997)

We present a tone reproduction operator that preserves visibility in high dynamic range scenes. Our method introduces a new histogram adjustment technique, based on the population of local adaptation luminances in a scene. To match subjective viewing experience, the method incorporates models for human contrast sensitivity, glare, spatial acuity, and color sensitivity. We compare our results to previous work and present examples of our techniques applied to lighting simulation and electronic photography.
Supplementary Figure S7. from Functional Role of a Novel Long Noncoding RNA <i>TTN-AS1</i> in Esophageal Squamous Cell Carcinoma Progression and Metastasis (2023)

&lt;p&gt;LncRNA-TTN-AS1 promotes EMT and abrogates the effects of miR-133b.&lt;/p&gt;
Carbon-carbon bond formation via directed 2-azonia-[3,3]-sigmatropic rearrangements. A new pyrrolidine synthesis (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-carbon bond formation via directed 2-azonia-[3,3]-sigmatropic rearrangements. A new pyrrolidine synthesisLarry E. Overman and Masa-Aki KakimotoCite this: J. Am. Chem. Soc. 1979, 101, 5, 1310–1312Publication Date (Print):February 1, 1979Publication History Published online1 May 2002Published inissue 1 February 1979https://pubs.acs.org/doi/10.1021/ja00499a058https://doi.org/10.1021/ja00499a058research-articleACS PublicationsRequest reuse permissionsArticle Views1094Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Nanometer‐Sized Molybdenum–Iron Oxide Capsule‐Surface Modifications: External and Internal (2007)

The cluster {(Mo)Mo5}12Fe(III)30 1 a present in compound 1 (cluster diameter approximately 2.3 nm), which belongs to the family of nanoscale spherical porous {(Mo)Mo5}12{Linker}30 capsules that allow a new type of nanochemistry inside their cavities as well as unprecedented aggregation processes under gaseous, solution, and solid-state conditions, is the starting material for the present investigation. In solution it reacts with LnCl3 x nH2O (Ln = Ce, Pr) thereby replacing six Fe(III) ions with Ln(III) ions to form compounds 2 (Ce) and 3 (Pr). During metal-cation exchange, some of the pentagonal {(Mo)Mo5O21(H2O)6}6- units, which are connected to the Fe(III) centers in 1 a, decompose, thus leading to a temporary capsule opening and uptake of the formed smaller molybdate units into the capsule cavities. In 2 and 3, the pentagonal units are connected via 24 Fe(III) and six Ln(III)-type linkers/spacers representing together the capsule skeletons, which are structurally well-defined in contrast to the capsule contents. The new capsules self-associate into single-layer blackberry-type structures, thus extending the variety of these types of assemblies; the assembly process, that is, the size of the final species, can be controlled by the pH, which allows the generation of differently sized nanoparticles. Magnetic properties of the two new nanomaterials 2 and 3 are also determined.
Equine locomotion: 3. The reproducibility of gait in Standardbred trotters (1980)

The reproducibility of certain gait characteristics was investigated in Standardbred trotters at a speed of 12.0 m/sec. Twenty-two horses were recorded twice on the same day and 4 of them on a third occasion 3 years later. The short-term reproducibility was shown to be very good. In the long-term study it was found that the stride length and duration of swing and stride had increased. This was interpreted as an effect of training.
Comparison of Mycobacterium 23S rRNA Sequences by High-Temperature Reverse Transcription and PCR (1995)

We describe a modified rRNA sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus Mycobacterium. We combined the sensitivity of the reverse transcriptase PCR for amplifying nanogram amounts of template rRNA material with the elevated extension temperatures used for the thermostable DNA polymerase from Thermus thermophilus. A 70 degrees C reverse transcription extension step permitted improved read-through of highly structured rRNA templates from members of the genus Mycobacterium, which have G+C contents of 66 to 71 mol%. The nucleic acid sequences of the amplified material were then determined by performing thermal cycle sequencing with alpha-33P-labeled primers, again with extension at 70 degrees C. Nonspecifically terminated bands were chased by using terminal deoxynucleotidyl transferase. Our method had a template requirement of nanogram amounts or less of purified RNA or 2,000 CFU of intact cells and had sufficient sensitivity so that lyophils obtained from the American Type Culture Collection could be used as source material. Sequences from a 250-nucleotide stretch of the 23S rRNA were aligned, and phylogenetic trees were evaluated by using the De Soete distance treeing algorithm and Rhodococcus bronchialis as the outgroup. Our 23S rRNA trees were compared with previously published 16S rRNA trees, including the comprehensive trees developed by the University of Illinois Ribosomal Database Project, and included 15 species not evaluated previously. Most of the groups were in general agreement and were consistent with relationships determined on the basis of biochemical characteristics, but some new relationships were also observed.
Preparation and Tribological Properties of Carbon-Coated WS2 Nanosheets (2019)

WS<sub>2</sub>-C is produced from a hydrothermal reaction, in which WS<sub>2</sub> nano-sheets are coated with carbon, using glucose as the carbon source. In order to investigate the tribological properties of WS<sub>2</sub>-C as a lubricant additive, WS<sub>2</sub>-C was modified by surfactant Span80, and friction tests were carried out on an MRS-10A four-ball friction and wear tester. The results show that Span80 can promote the dispersibility of WS<sub>2</sub>-C effectively in base oil. Adding an appropriate concentration of WS<sub>2</sub>-C can improve the anti-wear and anti-friction performance of the base oil. The friction coefficient reached its lowest point upon adding 0.1 wt % WS<sub>2</sub>-C, reduced by 16.7% compared to the base oil. Meanwhile, the wear scar diameter reached its minimum with 0.15 wt % WS<sub>2</sub>, decreasing by 26.45%. Moreover, at this concentration, the depth and width of the groove and the surface roughness on the wear scar achieved their minimum. It is concluded that WS<sub>2</sub>-C dispersed in oil could enter friction pairs to avoid their direct contact, thereby effectively reducing friction and wear. At the same time, WS<sub>2</sub>-C reacts with the friction matrix material to form a protective film, composed of C, Fe<sub>2</sub>O<sub>3</sub>, FeSO<sub>4</sub>, WO<sub>3</sub>, and WS<sub>2</sub>, repairing the worn surface.
Three-phase modular single stage full-bridge converter for switched mode power supplies (2013)

In this paper, the modular development of a three-phase single stage ac-dc power converter based on single-phase ac-dc isolated full-bridge converter for telecom power supply applications is presented. Three single-phase converter modules are series connected to form a three-phase converter system to achieve balanced three-phase currents. This paper analyses the operation of a modular converter in continuous conduction mode (CCM) of power factor correction. The performance of such a modular full-bridge converter system has been evaluated with simulation based on a MATLAB model and the results are also presented. The main objective of the proposed system is to reduce the number of stages and improve the power factor of the input ac mains current. The proposed scheme offers simple control strategy, electrically isolated output, single-stage power conversion, elimination of power transformer at the front end and inherent power factor correction (PFC) for medium rating power supplies.
Positive Sociology and Appreciative Empathy: History and Prospects (2014)

This paper explores the contributions of sociology (and overlapping disciplines such as anthropology, social policy, and cultural studies) to happiness scholarship from the Enlightenment through to the present day. Pre-20th century thinkers whose work led to the formation of social science tended to take the theme of happiness seriously as a central challenge of social scholarship. Over the past century, sociologists have made important contributions to understanding happiness, although its absence from textbooks, encyclopedias, and conferences suggests that happiness has never been a major theme in mainstream sociology. The discipline's role in happiness scholarship could be greatly strengthened through more systematic and explicit approaches, especially in qualitative research. These will doubtless be developed soon, as sociology catches up with the other social sciences (most notably psychology and economics) that have already made great progress in convincing general publics and politicians that something so elusive as happiness can be analysed and assessed in robust and illuminating ways. A ‘happiness lens’ is recommended as a way of making sociology more transparent regarding its contributions to understanding and promoting good societies and good lives. This lens complements pathologism with positivity; insists on empathic effort to respect first-person subjectivity; and promotes holism and lifecourse perspectives.
The Human-Companion Animal Bond (2004)

SUMMARY The author provides brief annotated references of basic resources on the "human-animal bond," the relationship between people and pets ("companion animals") throughout history. Types of human-animal relationships are very diverse, and the author offers examples of many, including animal-assisted therapies, pet-keeping, and training styles. KEYWORDS: Human-animal bondanimal-assisted therapyanimal abusepetstraining of animals
Studies on the Relation between Structures and Properties of Esteric Plasticizer. (1959)

ポリ塩化ビニル用可塑剤として広く用いられている,分子中に二つのエステル結合を有するエステル型可塑剤の構造と性能の関係について,アルキル鎖長,分枝,エステル結合の位置等の影響の検討を行った。結果は次のとおりであった。両端のアルキル鎖長は炭素数8より12に増加すると耐寒性は向上するが,可塑化効率,弾性的性質,機械的性質は低下し,炭素数16以上は混和性を失うにいたる。しかしながら,二つのエステル結合の間のアルキル鎖の長さは影響するところが小さい。エステル結合の方向による差は見られないが,二つのエステル結合が近づきすぎた場合,また一方に片寄ることは,長いアルキル鎖を生じ可塑剤の性能を低下させる。短い分枝は影響は少ないが,エステル結合につづく炭素に分枝をもつと,可塑剤の性能は低下の傾向を示す。
Configuration specification using a system structure language (1992)

A discussion is given on a system modelling language and supporting toolkit which was originally developed as part of the Eclipse software engineering environment. The authors address the modelling of large-scale systems made up of many different types of components. The language, called SySL (system structure language), enables system structure to be described at various levels of abstraction. A toolkit enables changes to the system descriptions to be made consistently, and automates the process of building an executable version of the described system. Using such a language and toolkit, a systems administrator can control the process of dynamically re-configuring a system in a much more effective way. SySL was developed in the first place to support the management of software configurations. The authors have extended the system and are using it in a project investigating the general problems of distributed systems management (G. Dean et al., 1992). The language is also the basis for the configuration language in a European collaborative project which is concerned with providing practical support for dynamic systems evolution. The authors describe some of the features of SySL and illustrate how the language can be used for system configuration specification using an example of a network of workstations. They then describe the toolkit which has been developed to support software configuration management using SySL and initial work on the use of the language in conjunction with a dynamic configuration manager.<
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Editorial: Biomaterials for Skin Wound Repair: Tissue Engineering, Guided Regeneration, and Wound Scarring Prevention (2021)

Although laypersons may not realize it, the skin or integument is the largest and heaviest organ of our body. It is of the utmost importance that as a boundary organ it keeps its integrity and functions. True, the skin's esthetic role crucially mediates social and emotional interactions. But there is much more than this: wounds due to any factor or agent impair the integument's barrier function allowing the entry of infectious agents and the concurrent loss of blood and interstitial fluid components. Wounds' severity is proportional to their extent and depth. Still other etiologic factors drive acute and chronic diseases also altering the skin's barrier. Most importantly, bidirectional pathophysiological links exist between the skin and internal organs. A diseased organ can alter skin's structure and function. For example, immune reactions to foreign or endogenous antigens or malignancy may drive a diffuse urticaria (hives). As an example of the reverse, extensive deep burn wounds both favor infections and release toxins inducing multiple organs failure and jeopardizing patients' survival.
Progress in Prevention and Treatment of Acute Kidney Injury (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Cancelling ECG Artifacts in EEG Using a Modified Independent Component Analysis Approach (2008)

We introduce a new automatic method to eliminate electrocardiogram (ECG) noise in an electroencephalogram (EEG) or electrooculogram (EOG). It is based on a modification of the independent component analysis (ICA) algorithm which gives promising results while using only a single-channel electroencephalogram (or electrooculogram) and the ECG. To check the effectiveness of our approach, we compared it with other methods, that is, ensemble average subtraction (EAS) and adaptive filtering (AF). Tests were carried out on simulated data obtained by addition of a filtered ECG on a visually clean original EEG and on real data made up of 10 excerpts of polysomnographic (PSG) sleep recordings containing ECG artifacts and other typical artifacts (e.g., movement, sweat, respiration, etc.). We found that our modified ICA algorithm had the most promising performance on simulated data since it presented the minimal root mean-squared error. Furthermore, using real data, we noted that this algorithm was the most robust to various waveforms of cardiac interference and to the presence of other artifacts, with a correction rate of 91.0%, against 83.5% for EAS and 83.1% for AF.
Multi-effective properties of homogentisic acid revealed in reactions with human hemoglobin and human erythrocytic hemoglobin. (2000)

Homogentisic acid (HGA) causes oxidation of human oxyhemoglobin and reduction of methemoglobin. The rate of oxidation of oxyhemoglobin by HGA is greatly accelerated in the presence of myo-inositol hexakis-phosphate (P6-inositol) or superoxide dismutase (SOD), but is inhibited in the presence of catalase. The reduction rate of methemoglobin by HGA is accelerated in the presence of P6-inositol but is greatly inhibited in the presence of SOD. It is suggested that the semiquinone and quinone form of HGA and oxygen radicals may be involved in the mechanism of oxido-reductive reactions of human hemoglobin with HGA. In addition, a new anodic hemoglobin found by isoelectric focusing electrophoresis was produced during the reaction of oxyhemoglobin with HGA. When human erythrocytes were exposed to HGA for several hours at 37 degrees C (pH 7.4), the anodic oxyhemoglobin (HGA-modified hemoglobin) and its half met-form hemoglobin [(alpha3+beta2+)2 of HGA-modified hemoglobin] were produced in significant amounts. HGA-modified hemoglobin was stably purified and showed increased oxygen affinity, absence of titratable sulfhydryl groups, and the absorption spectrum of normal oxyhemoglobin. Our results demonstrate that HGA shows multiple effects on human hemoglobin and erythrocytic hemoglobin, which is consistent with the evidence that HGA is involved in various pathological conditions such as arthritis and carcinogenesis in humans.
Taking silicon to the limit: challenges and opportunities (2004)

Silicon-based CMOS transistors can be scaled well into the sub-10nm regime. However, new materials and processes, in conjunction with advanced transistor structures, are needed for nanometer-scale MOSFETs to meet ITRS performance specifications. This paper discusses challenges for achieving target performance metrics at the end of the roadmap, and approaches to overcoming them.
Targeting microglia for the treatment of Alzheimer's Disease (2016)

While histological changes in microglia have long been recognized as a pathological feature of Alzheimer's disease (AD), recent genetic association studies have also strongly implicated microglia in the etiology of the disease. Coding and noncoding polymorphisms in several genes expressed in microglia-including APOE, TREM2, CD33, GRN, and IL1RAP-alter AD risk, and therefore could be considered as entry points for therapeutic intervention. Furthermore, microglia may have a substantial effect on current amyloid β (Aβ) and tau immunotherapy approaches, since they are the primary cell type in the brain to mediate Fc receptor-facilitated antibody effector function. In this review, we discuss the considerations in selecting microglial therapeutic targets from the perspective of drug discovery feasibility, and consider the role of microglia in ongoing immunotherapy clinical strategies. GLIA 2016;64:1710-1732.
<title>Characterization of the one-micron beam profile at the interface between the one-micron laser and the UV generation and transport subsystem</title> (1997)

The near field irradiance parameters at the interface between the one micron laser, the UV generation, and transport subsystem will be discussed. The test results obtained from the Beamlet and Nova lasers used to validate the mathematical models will be presented.
Design and Implementation of Long High-Rate QC-LDPC Codes and Its Applications to Optical Transmission Systems (2013)

In this paper, we present a novel class of long quasi-cyclic low-density parity-check (QC-LDPC) codes. Each of the codes in this class has a structure formed by concatenating single-parity-check codes and QC-LDPC codes of shorter lengths, which allows for efficient, high throughput encoder/decoder implementations. Using a code in this class, we design a forward error correction (FEC) scheme for optical transmission systems and present its high throughput encoder/decoder architecture. In order to demonstrate its feasibility, we implement the architecture on a field programmable gate array (FPGA) platform. We show by both FPGA-based simulations and measurements of an optical transmission system that the FEC scheme can achieve excellent error performance and that there is no significant performance degradation due to the constraint on its structure while getting an efficient, high throughput implementation is feasible.
Effect of Chemical Chaperones in Improving the Solubility of Recombinant Proteins in Escherichia coli (2011)

The recovery of active proteins from inclusion bodies usually involves chaotrope-induced denaturation, followed by refolding of the unfolded protein. The efficiency of renaturation is low, leading to reduced yield of the final product. In this work, we report that recombinant proteins can be overexpressed in the soluble form in the host expression system by incorporating compatible solutes during protein expression. Green fluorescent protein (GFP), which was otherwise expressed as inclusion bodies, could be made to partition off into the soluble fraction when sorbitol and arginine, but not ethylene glycol, were present in the growth medium. Arginine and sorbitol increased the production of soluble protein, while ethylene glycol did not. Production of ATP increased in the presence of sorbitol and arginine, but not ethylene glycol. A control experiment with fructose addition indicated that protein solubilization was not due to a simple ATP increase. We have successfully reproduced these results with the N-terminal domain of HypF (HypF-N), a bacterial protein which forms inclusion bodies in Escherichia coli. Instead of forming inclusion bodies, HypF-N could be expressed as a soluble protein in the presence of sorbitol, arginine, and trehalose in the expression medium.