gebhart/patrepeval huggingface dataset:
An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins (2013)

Determining the composition of messenger ribonucleoprotein (mRNP) particles is essential for a comprehensive understanding of the complex mechanisms underlying mRNA regulation, but is technically challenging. Here we present an RNA-based method to identify RNP components using a modified streptavidin (SA)-binding RNA aptamer termed S1m. By optimizing the RNA aptamer S1 in structure and repeat conformation, we improved its affinity for SA and found a 4-fold repeat of S1m (4×S1m) to be more efficient than the established MS2 and PP7 systems from bacteriophages. We then attached the AU-rich element (ARE) of tumor necrosis factor alpha (TNFα), a well-known RNA motif that induces mRNA degradation, via 4×S1m to a SA matrix, and used the resulting RNA affinity column to purify ARE-binding proteins (BPs) from cellular extracts. By quantitative mass spectrometry using differential dimethyl labeling, we identified the majority of established ARE-BPs and detected several RNA-BPs that had previously not been associated with AREs. For two of these proteins, Rbms1 and Roxan, we confirmed specific binding to the TNFα ARE. The optimized 4×S1m aptamer, therefore, provides a powerful tool for the discovery of mRNP components in a single affinity purification step.
Agile Evolution of Launch Vehicle Space Software Systems (2004)

This paper and presentation will examine how a successful space launch vehicle software system has evolved over the years and is successfully adopting leading edge improvement concepts. The Atlas launch vehicle has been a workhorse since the beginning of space exploration and in its current version, Atlas V, is an industry-leading provider of access to space. The paper will be set within the context of the Atlas system and software organization, and we view context as the key for successful evolution.
Phosphorylated Intermediate of the ATPase from the Plasma Membrane of Yeast (1981)

A purified preparation of the plasma-membrane ATPase from Saccharomyces cerevisiae was phosphorylated when incubated with [gamma-32P]ATP. The phosphoprotein formed has the characteristics of an enzyme intermediate because of its rapidity of phosphorylation and dephosphorylation. When the phosphorylated enzyme was analyzed by polyacrylamide gel electrophoresis in sodium dodecylsulfate only one band with a molecular weight of 100000 contained radioactivity. This band represented about 80% of the protein of the preparation and its enrichment in the course of the purification correlated with the increase in the specific ATPase activity. Both the ATPase reaction and the phosphorylation of the enzyme exhibited an apparent dissociation constant for the enzyme-ATP complex of 0.2 mM, further implicating the phosphoenzyme as an intermediate of the reaction. The sensitivity of the phosphoenzyme bond to alkaline pH and hydroxylamine indicate that it is an acylphosphate. From the maximum level of intermediate (0.7 nmol/mg) and the maximum ATPase activity at 30 degrees C (21 mumol x min-1 x mg-1) a turnover number of 30000 min-1 can be calculated. The level of phosphoenzyme was not affected by either the ATPase inhibitors vanadate and dicyclohexylcarbodiimide or by ADP. These results indicate that the yeast plasma-membrane ATPase has a subunit composition and reaction mechanism similar to the cation-pumping ATPases of animal plasma membranes.
Aging Face Recognition: A Hierarchical Learning Model Based on Local Patterns Selection (2016)

Aging face recognition refers to matching the same person's faces across different ages, e.g., matching a person's older face to his (or her) younger one, which has many important practical applications, such as finding missing children. The major challenge of this task is that facial appearance is subject to significant change during the aging process. In this paper, we propose to solve the problem with a hierarchical model based on two-level learning. At the first level, effective features are learned from low-level microstructures, based on our new feature descriptor called local pattern selection (LPS). The proposed LPS descriptor greedily selects low-level discriminant patterns in a way, such that intra-user dissimilarity is minimized. At the second level, higher level visual information is further refined based on the output from the first level. To evaluate the performance of our new method, we conduct extensive experiments on the MORPH data set (the largest face aging data set available in the public domain), which show a significant improvement in accuracy over the state-of-the-art methods.
Adaptive SINS/ANS/GNSS for Air-Launch Space Launcher: Algorithm Design and Performance Analysis (2007)

The paper deals with the development of the multirate information fusion algorithm for the integrated navigation system. The system is designed for air-launch space launcher used within the conversion programs for non-military space exploration. The integrated navigation system includes a strapdown inertial navigation system (strapdown INS, SINS), CCDbased strapdown astronavigation system (ANS), multi-antenna combined GPS/GLONASS receiver i.e. SINS/ANS/GNSS is under consideration. Loosely coupled SINS/GNSS architecture is used. The innovation-based adaptive Kalman filter (KF) fuses navigation solutions feeded by the above systems to produce a more accurate and reliable navigation solution in the presence of non-stationary Gaussian noise. SINS/ANS/GNSS errors are the states of linear time-varying (LTV) state-space system. KF estimates the system state vector. First-order Gauss-Markov processes are used to model errors time-correlation. Correlation matrices of error shaping filters are adjusted according to body dynamics and environment. KF measurements are constructed as SINS, ANS, and GNSS navigation solution differences. Lagrange polynomials are used to interpolate data from each system. SINS/ANS/GNSS performance is compared to that of loosely coupled SINS/GNSS in numerical simulations. INS/ANS/GNSS implementations are reviewed.
Instrumental Sources of Noise in a Pulsed Dye Laser Double Beam Spectrometer (1978)

Reducing the noise in the ratio of the signals for the two beams in a double beam spectrometer that uses a pulsed laser as a light source depends upon the ability of each beam to respond equally to all parts of the cross section of the laser beam. Optical components that are free of bulk and surface scattering sites are important. An opal glass filter placed near each detection system, in a position to fill its aperture, helps to improve the shot-to-shot reproducibility of the ratio. The best results are obtained when the spatial mode structure, or the cross section intensity pattern, of the laser is relatively reproducible.
On mean estimation for heteroscedastic random variables (2023)

Nous étudions le problème de l’estimation de la moyenne commune μ de n variables aléatoires symétriques indépendantes avec des écarts types différents et inconnus σ1≤σ2≤⋯≤σn. Nous montrons que, sous faibles hypothèses de régularité sur la distribution, il existe un estimateur adaptatif μˆ invariant par rapport aux permutations des éléments de l’échantillon qui satisfait à facteurs logarithmiques près et avec une grande probabilité |μˆ−μ|≲min{σm∗,n ∑ i=nnσi−1}, où l’indice m∗≲n satisfait m∗≈ σm∗∑ i=m∗nσi−1.
Comparison of Three Transcytotic Pathways for Distribution to Brain Metastases of Breast Cancer (2022)

Abstract Advances in drug treatments for brain metastases of breast cancer have improved progression free survival but new, more efficacious strategies are needed. Most chemotherapeutic drugs infiltrate brain metastases by moving between brain capillary endothelial cells, paracellular distribution, resulting in heterogeneous distribution, lower than that to systemic metastases. Herein, we tested three well-known transcytotic pathways through brain capillary endothelial cells as potential avenues for drug access: Transferrin receptor (TfR) peptide, Low density lipoprotein receptor 1 (LRP1) peptide, Albumin. Each was far-red labeled, injected into two hematogenous models of brain metastases, circulated for two different times, and their uptake quantified in metastases and uninvolved (nonmetastatic) brain. Surprisingly, all three pathways demonstrated distinct distribution patterns in vivo. Two were suboptimal: TfR distributed to uninvolved brain but poorly in metastases, while LRP1 was poorly distributed. Albumin distributed to virtually all metastases in both model systems, significantly greater than in uninvolved brain (P &lt;0.0001). Further experiments revealed that albumin entered both macrometastases and micrometastases, the targets of treatment and prevention translational strategies. Albumin uptake into brain metastases was not correlated with the uptake of a paracellular probe (biocytin). We identified a novel mechanism of albumin endocytosis through the endothelia of brain metastases consistent with clathrin-independent endocytosis (CIE), involving the neonatal Fc receptor (FcRn), galectin-3 (Gal-3) and glycosphingolipids. Components of the CIE process were found on metastatic endothelial cells in human craniotomies. The data suggest a reconsideration of albumin as a translational mechanism for improved drug delivery to brain metastases and possibly other CNS cancers. Statement of Significance Drug therapy for brain metastasis needs improvements. We surveyed transcytotic pathways as potential delivery systems in brain-tropic models and found that albumin has optimal properties. Albumin used a novel mechanism for endocytosis.
An Approach to Sensitivity Analysis of Computer Models: Part I—Introduction, Input Variable Selection and Preliminary Variable Assessment (1981)

AbstractThis is the first part of a two-part article presenting a statistical approach to the sensitivity analysis of computer models. Part I defines the objectives of sensitivity analysis and presents a computer model that is used for purposes of illustration in the paper. A discussion of the necessary considerations for selection of sample values of variables used as input to the model is given. Details are given for the identification of important variables when the computer output varies with time and when all input variables have been varied simultaneously. For this latter topic the importance of considering data transformation is explained.KeywordsComputer ModelsSensitivity Analysis Additional informationNotes on contributorsRonald L. ImanDr. Iman is a statistical consultant.Jon C. HeltonDr. Helton is an Associate Professor of Mathematics in the Department of Mathematics at Arizona State University and is currently on leave to Sandia.James E. CampbellDr. Campbell is a physicist and is now employed with Intera Environmental Consultants.
Spectroscopy and structure of jet-cooled .alpha.-methylstyrene (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectroscopy and structure of jet-cooled .alpha.-methylstyreneV. H. Grassian, Elliot R. Bernstein, Henry V. Secor, and Jeffrey I. SeemanCite this: J. Phys. Chem. 1990, 94, 17, 6691–6695Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August 1990https://pubs.acs.org/doi/10.1021/j100380a032https://doi.org/10.1021/j100380a032research-articleACS PublicationsRequest reuse permissionsArticle Views82Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Errata to “Codes and Designs Related to Lifted MRD Codes” [Feb 13 1004-1017] (2013)

In the above titled paper (ibid., vol. 59, no. 2, pp. 1004-1017, Feb. 2013), the matrix in Example 7 (p. 1014) is incorrect. The correct matrix is presented here.
Damascene Light‐Weight Metals (2010)

Abstract Besides widely investigated severely plastically deformed materials that are available in laboratory scale and size only, there is a high demand for semi‐finished products such as sheets and wires with similar mechanical properties. A damascene‐like technology applying swaging and bundling/swaging allows to deform TiNbAl composites up to a log. deformation strain of 8.4. Here, Al and Ti are used because of their low density, while Nb acts as diffusion barrier to prevent the formation of hardly deformable intermetallic phases. The obtained wires show an ufg microstructure with grain sizes of Ti and Al between 100 and 200 nm. In the cold‐worked condition the wires with a density of 4.0 g cm −3 reveal an ultimate tensile strength of 790 MPa.
Cell-Based Regenerative Endodontics for Treatment of Periapical Lesions: A Randomized, Controlled Phase I/II Clinical Trial (2020)

A randomized controlled phase I/II clinical trial was designed to evaluate the safety and efficacy of encapsulated human umbilical cord mesenchymal stem cells in a plasma-derived biomaterial for regenerative endodontic procedures (REPs) in mature permanent teeth with apical lesions. The trial included 36 patients with mature incisors, canines, or mandibular premolars showing pulp necrosis and apical periodontitis. Patients were randomly and equally allocated between experimental (REP) or conventional root canal treatment (ENDO) groups. On the first visit, cavity access and mechanical preparation of the root canal were performed. Calcium hydroxide medication was used, and the cavity was sealed. Three weeks later, patients were treated following their assigned protocol of ENDO or REP. Clinical follow-up examinations were performed at 6 and 12 mo. Categorical variables were evaluated by Fisher's exact test. Quantitative variables were compared using the Mann-Whitney test. The evolution over time of the percentage of perfusion units and the dimensions of lesion and cortical compromise were explored. After the 12-mo follow-up, no adverse events were reported, and the patients showed 100% clinical efficacy in both groups. Interestingly, in the REP group, the perfusion unit percentage measured by laser Doppler flowmetry revealed an increase from 60.6% to 78.1% between baseline and 12-mo follow-up. Sensitivity tests revealed an increase of the positive pulp response in the REP group at 12-mo follow-up (from 6% to 56% on the cold test, from 0% to 28% on the hot test, and from 17% to 50% on the electrical test). We present the first clinical safety and efficacy evidence of the endodontic use of allogenic umbilical cord mesenchymal stem cells encapsulated in a plasma-derived biomaterial. The innovative approach, based on biological principles that promote dentin-pulp regeneration, presents a promising alternative for the treatment of periapical pathology (ClinicalTrials.gov NCT03102879).
Molecular Structure of Nitro- and Nitrito-Copper Complexes as Reaction Intermediates in Electrochemical Reduction of Nitrite to Dinitrogen Oxide (1995)

Abstract Copper-nitrito and -nitro isomers, [Cu(ONO)(tpa)]PF6 and [Cu(NO2)(tpa)]PF6 (tpa = tris[(2-pyridyl)methyl]amine) were isolated and the molecular structures were determined by X-ray analysis. [Cu(ONO)(tpa)]PF6 (C18H18N5O2PF6Cu) crystallizes in the monoclinic space group P21/a with a = 13.374(2), b = 14.033(2), c = 13.455(2) Å, β = 119.10(1)°, V = 2206.5(6) Å3, and Z = 4. [Cu(NO2)(tpa)]PF6 (C18H18N5O2PF6Cu) crystallizes in the orthorhombic space group I/ba2 with a = 16.718(3), b = 17.554(3), c = 14.785(3) Å, V = 4338(1) Å3, and Z = 8. Those nitrito- and nitro-complexes exist as an equilibrium mixture in solutions. Electrochemical reduction of NO2− in the presence of [Cu(H2O)(tpa)](ClO4)2 at −0.4 V in H2O (pH 7.0) catalytically produced N2O with concomitant evolution of a small amount of NO via the nitro and nitrito adducts.
Reducing corneal toxicity of 5‐fluorouracil in the early postoperative period following glaucoma filtering surgery (1991)

To gain a better understanding of the relationship between postoperative 5-fluorouracil administration and corneal complications associated with its use, we analysed 43 consecutive filtering procedures in which it had been employed. Nine of the 43 eyes had corneal complications during that period. At the end of the three-month period, these corneal complications had all resolved. There was no correlation between postoperative intraocular pressure lowering and incidence of corneal complications. A low dose of 5-fluorouracil, and early recognition and treatment of corneal toxicity associated with its use may reduce corneal complications while maintaining filtration in eyes with poor surgical prognosis.
Studies of Tritylated Pentoses and 6-Deoxyhexoses. II. Trityl Ethers of Methyl a- and β-l-Rhamnopyranoside and Their Derivatives (1979)

Abstract Mono-O-trityl derivatives of methyl α- and β-l-rhamnopyranoside have been prepared and the structures established by PMR analysis.
Photochemical Control of DNA Decoy Function Enables Precise Regulation of Nuclear Factor κB Activity (2011)

DNA decoys have been developed for the inhibition of transcriptional regulation of gene expression. However, the present methodology lacks the spatial and temporal control of gene expression that is commonly found in nature. Here, we report the application of photoremovable protecting groups on nucleobases of nuclear factor κB (NF-κB) DNA decoys to regulate NF-κB-driven transcription of secreted alkaline phosphatase using light as an external control element. The NF-κB family of proteins is comprised of important eukaryotic transcription factors that regulate a wide range of cellular processes and are involved in immune response, development, cellular growth, and cell death. Several diseases, including cancer, arthritis, chronic inflammation, asthma, neurodegenerative diseases, and heart disease, have been linked to constitutively active NF-κB. Through the direct incorporation of caging groups into an NF-κB decoy, we were able to disrupt DNA:DNA hybridization and inhibit the binding of the transcription factor to the DNA decoy until UV irradiation removed the caging groups and restored the activity of the oligonucleotide. Excellent light-switching behavior of transcriptional regulation was observed. This is the first example of a caged DNA decoy for the photochemical regulation of gene expression in mammalian cells and represents an important addition to the toolbox of light-controlled gene regulatory agents.
A spider derived peptide, PnPP-19, induces central antinociception mediated by opioid and cannabinoid systems (2016)

Some peptides purified from the venom of the spider Phoneutria nigriventer have been identified as potential sources of drugs for pain treatment. In this study, we characterized the antinociceptive effect of the peptide PnPP-19 on the central nervous system and investigated the possible involvement of opioid and cannabinoid systems in its action mechanism. Nociceptive threshold to thermal stimulation was measured according to the tail-flick test in Swiss mice. All drugs were administered by the intracerebroventricular route. PnPP-19 induced central antinociception in mice in the doses of 0.5 and 1 μg. The non-selective opioid receptor antagonist naloxone (2.5 and 5 μg), μ-opioid receptor antagonist clocinnamox (2 and 4 μg), δ-opioid receptor antagonist naltrindole (6 and 12 μg) and CB1 receptor antagonist AM251 (2 and 4 μg) partially inhibited the antinociceptive effect of PnPP-19 (1 μg). Additionally, the anandamide amidase inhibitor MAFP (0.2 μg), the anandamide uptake inhibitor VDM11 (4 μg) and the aminopeptidase inhibitor bestatin (20 μg) significantly enhanced the antinociception induced by a low dose of PnPP-19 (0.5 μg). In contrast, the κ-opioid receptor antagonist nor-binaltorphimine (10 μg and 20 μg) and the CB2 receptor antagonist AM630 (2 and 4 μg) do not appear to be involved in this effect. PnPP-19-induced central antinociception involves the activation of CB1 cannabinoid, μ- and δ-opioid receptors. Mobilization of endogenous opioids and cannabinoids might be required for the activation of those receptors, since inhibitors of endogenous substances potentiate the effect of PnPP-19. Our results contribute to elucidating the action of the peptide PnPP-19 in the antinociceptive pathway.
Life sciences and materials: A successful marriage is possible (2000)

Although some companies are separating their life sciences and materials businesses, others are keeping these entities under the same corporate umbrella to take advantage of possible synergies at the biology/materials science interface. The following remarks, which provide some perspectives about DuPont's approach to this strategy, were delivered as a keynote address at a conference titled “Life Sciences and Materials … Successful Marriage or Divorce?” organized in Pisa, Italy, in October 1999, by the European Polymer Federation and the University of Pisa. © 2000 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 38: 667–678, 2000
Correlation energy extrapolation by intrinsic scaling. III. Compact wave functions (2004)

The information gained in the context of extrapolating the correlation energy by intrinsic scaling is used to shorten the full configurational expansions of electronic wave function without compromising their chemical accuracy. The truncations are accomplished by judiciously limiting the participation of the ranges of predetermined approximate sets of natural orbitals in the various excitation categories.
25. Incidence of thyroid uptake of 180 consecutive patients undergoing 18F-FDG PET/CT imaging: significance for unsuspected thyroid pathology (2003)

s of the 31st Annual Meeting of the British Nuclear Medicine Society, Manchester, UK, 29 April to 1 May 2003 - ONCOLOGY I
Broadband satellite system technologies for effective use of the 12-30 GHz radio spectrum (2002)

Broadband satellite systems are an integral part of communications technology, aiming to provide a wide range of services to a society that increasingly expects ubiquitous access to broadband telecommunication services. Key drivers behind the development of these systems include rapid growth in the use of the Internet, PC- and TV-based e-commerce and interactive broadcasting services. In the UK, broadband satellite service trials are currently being conducted by BT, amongst others, with a primary objective of providing services to areas where asymmetric digital subscriber line (ADSL) technology has not been deployed. Two key factors for the success of broadband satellite technology lie in the area of overcoming constraints associated with frequency spectrum and orbital resources. This paper provides a review of technologies employed in geostationary (GSO) and nongeostationary (NGSO) satellite networks designed for the provision of broadband services to fixed and portable ground terminals within the 12 to 30 GHz frequency range. After reviewing the system design parameters related to the use of the radio frequency spectrum, consideration is given to the issues involved in sharing spectrum between GSO and NGSO satellite networks.