gebhart/patrepeval huggingface dataset:
Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75 (2018)

Transglutaminase type 2 (TG2) is a multifunctional enzyme that plays a key role in mitochondria homeostasis under stressful cellular conditions. TG2 interactome analysis reveals an enzyme interaction with GRP75 (glucose-regulated protein 75). GRP75 localizes in mitochondria-associated membranes (MAMs) and acts as a bridging molecule between the two organelles by assembling the IP3R-GRP75-VDAC complex, which is involved in the transport of Ca<sup>2+</sup> from the endoplasmic reticulum (ER) to mitochondria. We demonstrate that the TG2 and GRP75 interaction occurs in MAMs. The absence of the TG2-GRP75 interaction leads to an increase of the interaction between IP3R-3 and GRP75; a decrease of the number of ER-mitochondria contact sites; an impairment of the ER-mitochondrial Ca<sup>2+</sup> flux; and an altered profile of the MAM proteome. These findings indicate TG2 is a key regulatory element of the MAMs.
A framework for natural animation of digitized models (2006)

We present a novel versatile, fast and simple framework to generate highquality animations of scanned human characters from input motion data. Our method is purely mesh-based and, in contrast to skeleton-based animation, requires only a minimum of manual interaction. The only manual step that is required to create moving virtual people is the placement of a sparse set of correspondences between triangles of an input mesh and triangles of the mesh to be animated. The proposed algorithm implicitly generates realistic body deformations, and can easily transfer motions between human erent shape and proportions. erent types of input data, e.g. other animated meshes and motion capture les, in just the same way. Finally, and most importantly, it creates animations at interactive frame rates. We feature two working prototype systems that demonstrate that our method can generate lifelike character animations from both marker-based and marker-less optical motion capture data.
RGS10 shapes the hemostatic response to injury through its differential effects on intracellular signaling by platelet agonists (2018)

Platelets express ≥2 members of the regulators of G protein signaling (RGS) family. Here, we have focused on the most abundant, RGS10, examining its impact on the hemostatic response in vivo and the mechanisms involved. We have previously shown that the hemostatic thrombi formed in response to penetrating injuries consist of a core of fully activated densely packed platelets overlaid by a shell of less-activated platelets responding to adenosine 5'-diphosphate (ADP) and thromboxane A<sub>2</sub> (TxA<sub>2</sub>). Hemostatic thrombi formed in RGS10<sup>-/-</sup> mice were larger than in controls, with the increase due to expansion of the shell but not the core. Clot retraction was slower, and average packing density was reduced. Deleting RGS10 had agonist-specific effects on signaling. There was a leftward shift in the dose/response curve for the thrombin receptor (PAR4) agonist peptide AYPGKF but no increase in the maximum response. This contrasted with ADP and TxA<sub>2</sub>, both of which evoked considerably greater maximum responses in RGS10<sup>-/-</sup> platelets with enhanced G<sub>q</sub>- and G<sub>i</sub>-mediated signaling. Shape change, which is G<sub>13</sub>-mediated, was unaffected. Finally, we found that free RGS10 levels in platelets are actively regulated. In resting platelets, RGS10 was bound to 2 scaffold proteins: spinophilin and 14-3-3γ. Platelet activation caused an increase in free RGS10, as did the endothelium-derived platelet antagonist prostacyclin. Collectively, these observations show that RGS10 serves as an actively regulated node on the platelet signaling network, helping to produce smaller and more densely packed hemostatic thrombi with a greater proportion of fully activated platelets.
Noble FeRAM technologies with MTP cell structure and BLT ferroelectric capacitors (2004)

A 16 Mb 1TIC FeRAM with a novel cell structure has been successfully developed with 0.25 /spl mu/m process technology using (Bi,La)/sub 4/Ti/sub 3/O/sub 12/ (BLT) capacitors for the first time. The developed FeRAM is highly scalable and reliable as a result of applying an MTP (merged top electrode and plate line) structure and BLT stacked capacitor, respectively.
Relationship Between Platelet Density and Number of Dense Bodies (1977)

Dense bodies are the storage site of 5-HT and their number might correlate with the different platelet populations. Mepacrine, a basic fluorescent dye, is a suitable tool for studying abnormalities in platelet 5-HT storage. The content of platelet DB can be readily estimated by examination under fluorescence microscope of platelets charged with mepacrine. In human platelets, a mean number of 6.3 DB/ platelet was found. This estimation has been performed in platelets of different densities separated on sucrose gradient : heavy platelets, in which 5-HT uptake is strongly inhibited by reserpine, had a mean number of 7 DB/platelet and none was empty of DB ; light platelets which are less active in regard to 5-HT and insensitive to reserpine, had less than 3 DB/platelet and most of them had a cytoplasmatic fluorescence. Thus the proportion of light and heavy platelets can be directly established by staining platelets with mepacrine. In view of this, platelets of two patients with an abnormal density distribution were studied for 5-HT and charged with mepacrine. The first presented an Hermansky-Pudlak syndrome (HPS), an increased proportion, of light platelets, a low 5-HT uptake insensitive to reserpine and a great reduction of mepacrine-stained DB. The other patient with a Bernard-Soulier syndrome had only heavy platelets ; 5-HT uptake in these large platelets was markedly increased and all platelets had more than 20 DB stained with mepacrine. These results confirm that there is a good relationship between platelet density and number of dense bodies.
Evaluation of relative GPS timing under jamming conditions (2012)

In this paper the performance of GPS timing under jamming conditions is evaluated in the context of a GPS jammer localization system that uses the Time-Difference-Of-Arrival (TDOA) technique. To investigate the performance of GPS timing receivers under jamming conditions, jammer signals of different types and powers were combined with real GPS signals and input into two timing receivers and the response of the PPS and frequency outputs measured. It was found that for at least 3 seconds after the jammer was turned on there was no noticeable drift. This is long enough to switch to a backup timing source or to perform a number of TDOA measurements. The implementation of post-processed time synchronization based on common-view satellite time transfer was also explored to examine the performance that can be expected when using the GPS signals embedded in the data captured for TDOA localization. The relative clock drift between two GPS receivers was then measured for 11 hours to see how Temperature Compensated Crystal Oscillators (TCXOs) behave over time. Using a linear fit to the relative clock drift values before simulated GPS outages, it was found that in the worst case a similar amount of holdover could be achieved as the timing receivers before the drift became significant. The performance of the timing receivers during a jammer localization field-trial was then analyzed. It was found that the TDOA localization could be improved significantly by correcting for timing offsets detected using the post-processing technique.
Abstract 5143: KDM3A promotes cell growth and cisplatin-resistance in ovarian cancer cells (2014)

Abstract Background: Ovarian cancer is the most lethal gynecological cancer affecting the women worldwide. One of the main challenging hurdles in ovarian cancer treatment is the high recurrence rate due to therapeutic resistance. Recent evidences showed that JmjC-domain (JMJD) containing histone demethylases plays a critical role in controlling gene expression during normal development and cancer progression. Aims: To determine whether histone demethylase plays a role in ovarian cancer progression and therapeutic resistance. Methods: We developed cisplatin-resistance ovarian cancer cell lines SKOV-3 and OVCAR-5 and isolated total RNA to screen the expression pattern of JMJD family genes by real-time RT-PCR. We performed stable knock-down of KDM3A by lentiviral mediated ShRNA transfection and studied its effects on cell proliferation and apoptosis by MTT assay and western blot analysis (PARP, caspase-3), respectively. We also determined the effect of KDM3A knock-down on cell cycle and apoptosis regulators by western blot. Results: Our observations indicated the differential expression of various JMJD family proteins between cisplatin-resistance and sensitive cell lines. To further evaluate our results, we also tested the expression of those differentially expressed JMJD proteins in a widely used cisplatin-resistance and sensitive A2780 cell lines. We observed that KDM3A/JMJD1A is consistently overexpressed in all the cisplatin-resistance cell lines studied. Next, we stably knock-down KDM3A in cisplatin-resistance OVCAR-5 and A2780 cell lines. More interestingly, we found that KDM3A depletion inhibited cell proliferation, induced apoptosis and enhanced the sensitivity to cisplatin treatment. Further, we confirmed that the important regulators of cell cycle and apoptosis including p21 and Bcl-2 were modulated by KDM3A knock-down. Conclusions: Our results indicate that KDM3A is a critical epigenetic factor required for the ovarian cancer growth by promoting cell proliferation and cisplatin-resistance. Our findings might have important clinical implications in targeting ovarian cancer cells by using specific histone demethylase inhibitors. Grant support: This work was supported by Carl and Roberta Deutsch, and Kelly Day Foundations. Citation Format: Sivakumar Ramadoss, Suvajit Sen, Gautam Chaudhuri, Robin Farias-Eisner. KDM3A promotes cell growth and cisplatin-resistance in ovarian cancer cells. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 5143. doi:10.1158/1538-7445.AM2014-5143
CONSTITUINTES QUÍMICOS E ATIVIDADE INSETICIDA DE Miconia ferruginata (2017)

Phytochemical investigation of the leaf extracts from Miconia ferruginata (Melastomataceae) led to the isolation and characterization of the flavonoids 5,6,7-trihydroxy-4'-methoxyflavone, 5-hydroxy-7,4'-dimethoxy-8-methylflavone and 5,7,4'-trihydroxy-6,8-dimetilflavone, triterpenes ursolic acid and oleanolic acid, in addition to the steroids β-sitosterol and stigmasterol. The structures of these compounds were proposed by spectroscopic analysis and comparison with literature data. This paper also describes the insecticidal effects of leaf extracts from M. ferruginata to one of the main pests of maize, the fall armyworm (Spodoptera frugiperda). The ingestion bioassays, the leaf extracts showed an elongation of the larval stage of 16.56 days as compared to the control.
Optimal phase kernels for time-frequency analysis (2002)

We consider the design of kernels for time-frequency distributions through the phase, rather than amplitude, response. While phase kernels do not attenuate troublesome cross-components, they can translate them in the time-frequency plane. In contrast to previous work on phase kernels that concentrated on placing the cross-components on top of the auto-components, we set up a "don't care" region and place the cross-components there. The close connections between optimal allpass kernels and optimal lowpass kernels provide valuable insight into signal-dependent time-frequency analysis.
Compiler-generated communication for pipelined FPGA applications (2003)

In this paper, we describe a set of compiler analyses and an implementation that automatically map a sequential and un-annotated C program into a pipelined implementation, targeted for an FPGA with multiple external memories. For this purpose, we extend array data-flow analysis techniques from parallelizing compilers to identify pipeline stages, required inter-pipeline stage communication, and opportunities to find a minimal program execution time by trading communication overhead with the amount of computation overlap in different stages. Using the results of this analysis, we automatically generate application-specific pipelined FPGA hardware designs. We use a sample image processing kernel to illustrate these concepts. Our algorithm finds a solution in which transmitting a row of an array between pipeline stages per communication instance leads to a speedup of 1.76 over an implementation that communicates the entire array at once.
The intermittent excitation of geodesic acoustic mode by resonant Instanton of electron drift wave envelope in L-mode discharge near tokamak edge (2021)

There are two distinct phases in the evolution of drift wave envelope in the presence of zonal flow. A long-lived standing wave phase, which we call the Caviton, and a short-lived traveling wave phase (in radial direction) we call the Instanton. Several abrupt phenomena observed in tokamaks, such as intermittent excitation of geodesic acoustic mode (GAM) shown in this paper, could be attributed to the sudden and fast radial motion of Instanton. The composite drift wave — zonal flow system evolves at the two well-separate scales: the micro-scale and the meso-scale. The eigenmode equation of the model defines the zero-order (micro-scale) variation; it is solved by making use of the two-dimensional (2D) weakly asymmetric ballooning theory (WABT), a theory suitable for modes localized to rational surface like drift waves, and then refined by shifted inverse power method, an iterative finite difference method. The next order is the equation of electron drift wave (EDW) envelope (containing group velocity of EDW) which is modulated by the zonal flow generated by Reynolds stress of EDW. This equation is coupled to the zonal flow equation, and numerically solved in spatiotemporal representation; the results are displayed in self-explanatory graphs. One observes a strong correlation between the Caviton-Instanton transition and the zero-crossing of radial group velocity of EDW. The calculation brings out the defining characteristics of the Instanton: it begins as a linear traveling wave right after the transition. Then, it evolves to a nonlinear stage with increasing frequency all the way to 20 kHz. The modulation to Reynolds stress in zonal flow equation brought in by the nonlinear Instanton will cause resonant excitation to GAM. The intermittency is shown due to the random phase mixing between multiple central rational surfaces in the reaction region.
Error-free transmission of 8 WDM channels at 10 Gbit/s using comb generation in a quantum dash based mode-locked laser (2008)

Error-free transmission over 50km single mode fiber of 8 WDM ITU channels with 100GHz channel spacing is demonstrated at 10Gbit/s by using comb generation in a quantum dash mode-locked laser.
Adaptations to chronic rapamycin in mice (2016)

Rapamycin inhibits mechanistic (or mammalian) target of rapamycin (mTOR) that promotes protein production in cells by facilitating ribosome biogenesis (RiBi) and eIF4E-mediated 5'cap mRNA translation. Chronic treatment with encapsulated rapamycin (eRapa) extended health and life span for wild-type and cancer-prone mice. Yet, the long-term consequences of chronic eRapa treatment are not known at the organ level. Here, we report our observations of chronic eRapa treatment on mTORC1 signaling and RiBi in mouse colon and visceral adipose. As expected, chronic eRapa treatment decreased detection of phosphorylated mTORC1/S6K substrate, ribosomal protein (rpS6) in colon and fat. However, in colon, contrary to expectations, there was an upregulation of 18S rRNA and some ribosomal protein genes (RPGs) suggesting increased RiBi. Among RPGs, eRapa increases rpl22l1 mRNA but not its paralog rpl22. Furthermore, there was an increase in the cap-binding protein, eIF4E relative to its repressor 4E-BP1 suggesting increased translation. By comparison, in fat, there was a decrease in the level of 18S rRNA (opposite to colon), while overall mRNAs encoding ribosomal protein genes appeared to increase, including rpl22, but not rpl22l1 (opposite to colon). In fat, there was a decrease in eIF4E relative to actin (opposite to colon) but also an increase in the eIF4E/4E-BP1 ratio likely due to reductions in 4E-BP1 at our lower eRapa dose (similar to colon). Thus, in contrast to predictions of decreased protein production seen in cell-based studies, we provide evidence that colon from chronically treated mice exhibited an adaptive 'pseudo-anabolic' state, which is only partially present in fat, which might relate to differing tissue levels of rapamycin, cell-type-specific responses, and/or strain differences.
Synthesis and Characterization of BrФnsted-Acidic Ionic Liquids (2010)

Five kinds of BrФnsted-acidic ionic liquids with different nitrogen-contained chemical groups and different anions were synthesized,and were respectively characterized by nuclear magnetic resonance(NMR),infrared spectrum(IR),electrospray ionization mass spectrum(ESI-MS)and thermogravimetry(TG).It was found that ten kinds of ionic liquids were synthesized,whose structures were consistent with the designed ones.And all of their purities were more than 95%.These ionic liquids possess high thermal stability and wide liquid range,and their decomposition temperatures are higher than 300℃ except that the tempetature of [MPy]NO3 is 199℃ by thermogravimetry.
Radiological and Clinical Outcomes of Balloon Kyphoplasty versus Radiofrequency Kyphoplasty in the Treatment of Vertebral Compression Fractures (2018)

Both BKP and RFK had comparable beneficial clinical and radiological effects in the treatment of vertebral compression fractures. However, neither the actual extent of height restoration nor its loss seems to affect the marked pain relief.
NASA's exploration agenda and capability engineering (2006)

NASA is using model-based languages and risk analysis methodologies to raise software development to the level of hardware development. Ultimately, it hopes to achieve a fusion of systems and software engineering by replacing conventional software development techniques with capability engineering, which focuses on a system's full set of functionalities.
STUDIES ON THE RENAL CONCENTRATING MECHANISM. III. EFFECT OF HEAVY EXERCISE (1959)

Heavy exercise decreases urine flow, sodium excretion, glomerular filtration rate and effec- tive renal blood flow in man (1-8). In addition, albumin, casts and red blood cells are often found in the urine sediment after exercise (9-12). Since the renal concentrating ability is a sensi- tive measure of renal impairment, the present study was undertaken to determine the effect of exercise on this function. Heavy exercise was found to impair concentrating ability in hydropenic normal men. This impairment was ob- served when the subjects exercised without prior solute loading and also during a small osmotic diuresis, but was not found when the subjects exercised during a large osmotic diuresis.
Determining optimal surface roughness of TiO2 blasted titanium implant material for attachment, proliferation and differentiation of cells derived from human mandibular alveolar bone (2001)

In the complex process of bone formation at the implant-tissue interface, implant surface roughness is an important factor modulating osteoblastic function. In this study, primary cultures of osteoblast-like cells, derived from human mandibular bone, were used. The aim was to examine the effect of varying surface roughness of titanium implant material on cellular attachment, proliferation and differentiation. A recognized method of increasing surface roughness and enlarging the surface area of titanium implants is by blasting with titanium dioxide particles: the four specimen types in the study comprised surfaces which were machine-turned only, or blasted after turning, with 63-90 microm, 106-180 microm, or 180-300 microm TiO(2) particles, respectively. The specimens were analyzed by scanning electron microscopy and confocal laser scanning. The turned samples had the smoothest surfaces: average height deviation (S(a)) of 0.20 microm. The roughest were those blasted with 180-300 microm particles, S(a) value 1.38 microm. Blasting with intermediate particle sizes yielded S(a) values of 0.72 microm and 1.30 microm, respectively. Cell profile areas were measured using a semiautomatic interactive image analyzer. Figures were expressed as percentage of attachment. DNA synthesis was estimated by measuring the amount of [(3)H]-thymidine incorporation into trichloroacetic acid (TCA) insoluble cell precipitates. The specific activity of alkaline phosphatase was assayed using p-nitrophenylphosphate as a substrate. The ability of the cells to synthesize osteocalcin was investigated in serum-free culture medium using the ELSA-OST-NAT immunoradiometric kit. After 3 h of culture, the percentage of cellular attachment did not differ significantly between specimens blasted with 180-300 micromparticles and the turned specimens. All blasted surfaces showed significantly higher [(3)H]-thymidine incorporation than the turned surfaces (P<0.05), with the highest on the surfaces blasted with 180-300 microm particles. Osteocalcin synthesis by the cells in response to stimulation by 1,25(OH)2D3, was also significantly greater (P<0.05) on the surfaces blasted with TiO(2) particles. However, analysis of alkaline phosphatase activity disclosed no significant differences among the four surface modifications. It is concluded that in this cellular model, the proliferation and differentiation of cells derived from human mandibular bone is enhanced by surface roughness of the titanium implant. However, increasing the size of the blasting particles to 300 microm does not further increase the initial attachment of the cells compared to turned surfaces and those blasted with 63-90 microm particles.
Spectrum Monitoring Using Energy Ratio Algorithm For OFDM-Based\n Cognitive Radio Networks (2015)

This paper presents a spectrum monitoring algorithm for Orthogonal Frequency\nDivision Multiplexing (OFDM) based cognitive radios by which the primary user\nreappearance can be detected during the secondary user transmission. The\nproposed technique reduces the frequency with which spectrum sensing must be\nperformed and greatly decreases the elapsed time between the start of a primary\ntransmission and its detection by the secondary network. This is done by\nsensing the change in signal strength over a number of reserved OFDM\nsub-carriers so that the reappearance of the primary user is quickly detected.\nMoreover, the OFDM impairments such as power leakage, Narrow Band Interference\n(NBI), and Inter-Carrier Interference (ICI) are investigated and their impact\non the proposed technique is studied. Both analysis and simulation show that\nthe \\emph{energy ratio} algorithm can effectively and accurately detect the\nappearance of the primary user. Furthermore, our method achieves high immunity\nto frequency-selective fading channels for both single and multiple receive\nantenna systems, with a complexity that is approximately twice that of a\nconventional energy detector.\n