gebhart/patrepeval huggingface dataset:
Supplementary Figure 3 from A Novel Human Dynactin-Associated Protein, dynAP, Promotes Activation of Akt, and Ergosterol-Related Compounds Induce dynAP-Dependent Apoptosis of Human Cancer Cells (2023)

Supplementary Figure 3 from A Novel Human Dynactin-Associated Protein, dynAP, Promotes Activation of Akt, and Ergosterol-Related Compounds Induce dynAP-Dependent Apoptosis of Human Cancer Cells
Evolution of metabolic pathways (2000)

The role of natural products in evolution (B.B. Jarvis). The evolution of phenolics in plants (H.A. Stafford). The family of chalcone synthase-related proteins: functional diversity and evolution (J. Schroder). Macrolide and polyether polyketides: biosynthesis and molecular diversity (Y.S. Tsantrizos, X.-S. Yang). Biochemical, molecular genetic, and evolutionary aspects of defense-related terpenoid metabolism in conifers (J. Bohlmann et al.). Function and evolution of plant cytochrome P450 (R.A. Kahn, F. Durst). The biosynthesis, degradation, transport and possible function of cyanogenic glucosides (P.R. Jones et al.). Two-oxoacid-dependent dioxygenases: inefficient enzymes or evolutionary driving force? (A.G. Prescott). Evolution of acyltransferase genes: origin and diversification of the BAHD superfamily of acyltransferases involved in secondary metabolism (B. St-Pierre, V. De Luca). Glycosyltransferases involved in plant secondary metabolism (T. Vogt). The methyltransferase gene superfamily: a tree with multiple branches (R.K. Ibrahim, I. Muzac). Brassinosteroids - structures, analysis and synthesis (J. Schmidt et al.). Recent advances in molecular genetic studies of the functions of brassinolide, a steroid hormone in plants (Z.-Y. Wang, J. Chory). Plant soluble sulfotransferases: structural and functional similarity with mammalian enzymes (F. Marsolais et al.).
P2851Transvenous lead placement in the setting of venous obstruction: outcomes of tunneling technique versus venoplasty (2019)

Abstract Background Venous stenosis is a well-recognized complication of transvenous leads when patients require lead revisions or device upgrade. In these cases, balloon venoplasty or alternative venous access with subcutaneous tunneling can be performed. Purpose Compare the outcomes of venoplasty vs. tunneling in patients requiring additional transvenous lead implantation. Methods A single center retrospective cohort study of all patients undergoing lead implantation/revision requiring venoplasty or tunneling from 2005–2017. Chi-squared and Wilcoxon Rank-Sum tests were used to compare categorical and continuous variables. Results Ninety five patients met our inclusion criteria (60 venoplasty &amp; 35 tunneling). There was no difference in procedure success rates (p-value=0.98). Tunneling was associated with less fluoroscopy time but higher incidence of acute complications (0% vs. 23%, p-value=0.002) most requiring invasive intervention and/or blood product transfusion. Long term outcomes were comparable and related to lead failure or infection. Outcomes for tunneling vs. venoplasty Variable Tunneling (n=35) Venoplasty (n=60) p-value Age Y 67 (23–84) 70 (29–91) 0.25 Male (%) 24 (69%) 41 (68%) 0.98 Number of existing leads 2.1 (1–4) 2.4 (0–4) 0.1 Age of oldest lead 7.0 (0–21) 7.2 (0–33) 0.73 Number of new leads 1.2 (1–3) 1.4 (1–3) 0.26 Fluoroscopy time (SD) min 29.2 (21.3) 39.7 (21.5) 0.012 Procedure success (%) 35 (100%) 55 (92%) 0.9 Acute complications (%) 8 (23%)1 0 (0%) 0.0002 Pocket hematoma/bleed 4 (11%)2 Hemothorax 2 (6%)3 Pericarditis 1 (3%) Lead compromise 1 (3%) Follow up M 18 (0–76) 28 (0–98) N/A Long term lead issues (%) 6 (17%) 10 (17%) 0.95 Long term complication requiring intervention 4 (11%) 8 (13%) N/A Procedure success: functional lead placed as a result of venoplasty or tunneling. 1Two patients required blood products. 2Two patients required invasive intervention. 3One patient required chest tube and the other ICU admission. Conclusions Balloon venoplasty is associated with similar rates of success and a less incidence of acute complications when compared subcutaneous tunneling. Acknowledgement/Funding NIH T32 Training Grant HL07111-40
Hm1 muscarinic cholinergic receptor internalization requires a domain in the third cytoplasmic loop. (1992)

Selected regions of the Hm1 muscarinic cholinergic receptor were mutated to analyze the molecular mechanisms of agonist-induced receptor internalization (or sequestration). The wild-type and mutant Hm1 genes were expressed, using pSG5, in U293 human kidney cells. Whereas surface receptor density measured with the polar tracer N-[3H]methylscopolamine was rapidly reduced by carbachol exposure, total receptor content measured with [3H]quinuclidinyl benzilate did not decline for at least 24 h, indicating the absence of extensive receptor down-regulation in U293 cells. Carbachol stimulation of phosphatidylinositol turnover paralleled receptor internalization, both with EC50 values of 10-20 microM. Furthermore, a D71N point mutation that prevented receptor activation also abolished carbachol-induced receptor internalization, indicating that receptor activation (but not necessarily second messenger stimulation) was required for internalization. Truncation of the COOH-terminal tail (K447 trunc) and point mutations of several potential Ser and Thr phosphorylation sites to Ala failed to affect receptor activation and internalization. In contrast, partial deletions of the third intracellular loop (i3) (Tyr208-Thr366) resulted in receptor mutants deficient in agonist-induced receptor internalization/sequestration. Various deletions caused either complete loss of internalization (d 232-358) or impaired internalization, ranging from 10 to 30% over 2 h, whereas wild-type Hm1 internalized to approximately 50%. Whereas the reason for the observed differences among the deficient deletion mutants remains unclear, the initial rate of N-[3H]methylscopolamine binding loss from the cell surface was much slower than that of wild-type Hm1 in each case. The deletion of only one single domain, 284-292 (SMESLTSSE), in the middle of i3 was consistently associated with impaired internalization. Domain 284-292 is partially conserved among closely related muscarinic receptors, whereas most of the remainder of i3 is not (except for the i3 membrane junctions), and similar Ser- and Thr-rich regions are present in many other G protein-coupled receptors. We propose that a small receptor domain in the middle of the i3 loop of Hm1 is involved in agonist-induced receptor internalization.
Artificial diffuser for Fourier transform hologram recording (1980)

A novel artificial diffuser for Fourier transform hologram recording is proposed, and its imaging and spectral properties are analyzed. This new diffuser gives high SNR and resolution characteristics to the holographically reconstructed images and high reliability and redundancy characteristics to the system where holograms are used as information storing media. These characteristics are confirmed by experiments.
Characteristics of proteins in juice and wine produced from Semillon grapes (2003)

The separation and characterization of proteins from Semillon grape juice and wine produced from it were carried out. Juice and wine proteins were isolated by the ammonium sulfate salting-out method, and futher fractionated and purified by Superdex 75 HR column chromatography.
Stability Assessment of Four Chimeric Proteins for Human Chagas Disease Immunodiagnosis (2021)

The performance of an immunoassay relies on antigen-antibody interaction; hence, antigen chemical stability and structural integrity are paramount for an efficient assay. We conducted a functional, thermostability and long-term stability analysis of different chimeric antigens (IBMP), in order to assess effects of adverse conditions on four antigens employed in ELISA to diagnose Chagas disease. ELISA-based immunoassays have served as a model for biosensors development, as both assess molecular interactions. To evaluate thermostability, samples were heated and cooled to verify heat-induced denaturation reversibility. In relation to storage stability, the antigens were analyzed at 25 °C at different moments. Long-term stability tests were performed using eight sets of microplates sensitized. Antigens were structurally analyzed through circular dichroism (CD), dynamic light scattering, SDS-PAGE, and functionally evaluated by ELISA. Data suggest that IBMP antigens are stable, over adverse conditions and for over a year. Daily analysis revealed minor changes in the molecular structure. Functionally, IBMP-8.2 and IBMP-8.3 antigens showed reactivity towards anti-<i>T. cruzi</i> antibodies, even after 72 h at 25 °C. Long-term stability tests showed that all antigens were comparable to the control group and all antigens demonstrated stability for one year. Data suggest that the antigens maintained their function and structural characteristics even in adverse conditions, making them a sturdy and reliable candidate to be employed in future in vitro diagnostic tests applicable to different models of POC devices, such as modern biosensors in development.
Securing inductively-coupled communication (2012)

Communication over inductively-coupled links is becoming prevalent in service delivery for medical, financial, and physical security applications and so there is a growing need to prevent eavesdropping. This paper presents circuit-theoretic and communication-theoretic models of inductively-coupled communication systems. Due to coupling, the presence of an eavesdropper detunes the transfer function between the legitimate users. It is shown this detuning can be detected to reveal the presence of the eavesdropper. Further, if capacity-approaching codes are employed, neither the eavesdropper nor the legitimate receiver are able to reconstruct the transmitted message with low error probability, effectively destroying the message. Building on this insight, a coding-based secure communication protocol for inductively-coupled communication, inspired by quantum key distribution, is developed. The notion of security is defined operationally in terms of probabilities rather than through traditional notions of equivocation.
A 1.8-V 67-mW 10-bit 100-MS/s pipelined ADC using time-shifted CDS technique (2004)

A time-shifted correlated double sampling (CDS) technique is proposed in the design of a 10-bit 100-MS/s pipelined ADC. This technique significantly reduces the finite opamp gain error without compromising the conversion speed, allowing the active opamp blocks to be replaced by simple cascoded CMOS inverters. Both high-speed and low-power operation is achieved without compromising the accuracy requirement. An efficient common-mode voltage control is introduced for pseudodifferential architecture which can further reduce power consumption. Fabricated in a 0.18-/spl mu/m CMOS process, the prototype 10-bit pipelined ADC occupies 2.5 mm/sup 2/ of active die area. With 1-MHz input signal, it achieves 65-dB SFDR and 54-dB SNDR at 100MS/s. For 99-MHz input signal, the SFDR and SNDR are 63 and 51 dB, respectively. The total power consumption is 67 mW at 1.8-V supply, of which analog portion consumes 45 mW without any opamp current scaling down the pipeline.
Eosinophil depletion protects mice from tongue squamous cell carcinoma induced by 4-nitroquinoline-1-oxide. (2014)

These results suggest that eosinophils might be responsible for the deleterious outcome of experimental tongue carcinogenesis, given that their ablation protects mice from OSCC.
4D measurement and analysis of plantar deformation during walking and running (2011)

Understanding of deformation of the plantar part of the foot during running is important for shoe insert design. In recent years, new technologies for 4D measurement (time-sequential 3D shapes) hav...
Training data selection and optimization for EUV lithography deep learning models (2023)

Numerous recent approaches have proven the efficacy of deep learning as a fast and efficient surrogate for various lithography simulation use cases. However, a drawback of such approaches is the requirement of large amounts of data, which is often difficult to obtain at advanced nodes. Different means to alleviate the data demands of deep learning models have been devised, such as transfer learning from different technology nodes and active data selection. Active data selection techniques tend to require large amounts of data to optimize and select. In our previous work, we devised a more efficient implementation of transfer learning and detailed numerous applications for EUV lithography. In this work, we expand on the data efficiency enhancements with domain knowledge-based data selection and the use of alternative data generated by different modeling approaches.
Detecting receiver attacks in VRTI-based device free localization (2012)

Variance-based Radio Tomographic Imaging (VRTI) is an emerging technology that locates moving objects in areas surrounded by simple and inexpensive wireless sensor nodes. VRTI uses human motion induced variation in RSS and spatial correlation between link variations to locate and track people. An artificially induced power variations in the deployed network by an adversary can introduce unprecedented errors in localization process of VRTI and, given the critical applications of VRTI, can potentially lead to serious consequences including loss of human lives. In this paper, we tackle the problem of detecting malicious receivers that report false RSS values to induce artificial power variations in a VRTI system. We use the term “Receiver Attack” to refer to such malicious power changes. We use a combination of statistical hypothesis testing and heuristics to develop real-time methods to detect receiver attack in a VRTI system. Our results show that we can detect receiver attacks of reasonable intensity and identify the source(s) of malicious activity with very high accuracy.
Fully Digitally Guided Implant Surgery Based on Magnetic Resonance Imaging (2019)

MRI in combination with the presented workflow may be used in edentulous cases for guided implant surgery. Further studies are needed to prove the promising accuracy of this alternative approach in clinical trials.
Continuous Cultivation of the Diatom Nitzschia laevis for Eicosapentaenoic Acid Production: Physiological Study and Process Optimization (2002)

The continuous cultures of the diatom Nitzschia laevis were performed at different dilution rates (D) and feed glucose concentrations (S(0)) to investigate cellular physiological responses and its production potential of eicosapentaenoic acid (EPA). Steady-state cell dry weight, residual glucose concentration, cell growth yield, specific glucose consumption rate, and fatty acid profiles were investigated within the range of D from 0.1 to 1.0 day(-1) (S(0) fixed at 20 g/L) and the range of S(0) from 5 to 35 g/L (D fixed at 0.3 day(-1)), respectively. The highest EPA productivity of 73 mg L(-1) day(-1) was obtained at D = 0.5 day(-1) and S(0) = 20 g/L. However, when the continuous culture achieved high productivities of EPA at certain dilution rates and feed glucose concentrations, glucose in the feed could not be consumed completely. Accordingly, the continuous culture was evaluated in terms of both EPA productivity (P) and glucose assimilation efficiency (E). The parameter eta, defined as the product of P and E, was used as an overall performance index. Since eta is a function of the two independent variables D and S(0), we employed a central composite design to optimize D and S(0) for the highest eta value. Based on the experimental results of the design, a second-order polynomial equation was established to represent the relationship between eta and D and S(0). The optimal values of D and S(0) were subsequently determined as 0.481 day(-1) and 15.56 g/L, respectively by the empirical model. The verification experiment confirmed the validity of the model. Under the optimal conditions, eta value reached 46.5 mg L(-1) day(-1), suggesting a considerably high efficiency of the continuous culture of N. laevis in terms of EPA production and glucose utilization.
Chemotherapy-induced allodinia: neuroprotective effect of acetyl-L-carnitine. (2005)

Considering the absence of any satisfactory treatment currently available for CIPN in a clinical setting, these are important observations, opening up the possibility of using ALC to treat a wide range of patients who have undergone chemotherapy and developed sensory peripheral neuropathy.
Human Bone Marrow Subpopulations Sustain Human Islet Function and Viability In vitro (2015)

These results suggest that BM and its derived M and E cells enable to support human islet β-cell function. However, only BM can sustain the capability of β-cell self regeneration through initiating β-cell transcriptional factors but not individual E and M cells suggesting pure E and M cells less supportive for islet long-term survival <i>in vitro</i>.
Hydrazine-mediated one-pot amination-oxidation reaction: facile synthesis of 4-amino-.beta.-carbolines and 4-aminoisoquinolines (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrazine-mediated one-pot amination-oxidation reaction: facile synthesis of 4-amino-.beta.-carbolines and 4-aminoisoquinolinesMark L. Trudell, N. Fukada, and J. M. CookCite this: J. Org. Chem. 1987, 52, 19, 4293–4296Publication Date (Print):September 1, 1987Publication History Published online1 May 2002Published inissue 1 September 1987https://pubs.acs.org/doi/10.1021/jo00228a025https://doi.org/10.1021/jo00228a025research-articleACS PublicationsRequest reuse permissionsArticle Views602Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Mutagen treatment as a means for selecting immunogenic variants from otherwise poorly immunogenic malignant murine tumors. (1983)

The selection of nontumorigenic (tum-), highly immunogenic variants from four different tumorigenic but poorly immunogenic murine tumors by mutagen treatment and cloning is described. Several factors were found to determine the successful nature of this selection procedure including: the nature of the tumor used; the nature of the mutagen; the number of mutagen treatments; and the time at which cloning is performed after treatment.

In some cases, e.g. , the TA3 adenocarcinoma or the BALB/c SS1 spontaneous mammary adenoacanthoma, a single treatment with ethyl methanesulfonate or N -methyl- N ′-nitro- N -nitrosoguanidine led to a very high frequency of tum- clones, whereas in others, e.g. , the MDAY-D2 tumor line, no stable tum- clones were obtained. The immunogenic clones selected were always immunologically cross-reactive with the parent tumor from which they were derived and were found to protect the murine host against challenge with the parent tumor in vivo . Thus, the cloned immunogenic variants share an antigen with the parent tumor. Additional evidence, however, suggested that each clone also expresses a new private or unique antigen. The frequency of immunogenic variant selection ranged from a low of 6% to a high of 95%. In some cases in which the frequencies were very high, cloning was not required to reveal the tum- phenotype. Finally, we also noted that selection for drug resistance, e.g. , resistance to 6-thioguanine, after mutagenesis could have an enhancing effect on the generation of highly immunogenic tum- clones. The results show that the immunogenicity of poorly immunogenic tumors, including those of spontaneous origin, can be dramatically enhanced by appropriate mutagen treatments but that there is considerable variation in the ease with which highly immunogenic variants can be obtained.
Dynamic Electrode–Electrolyte Intermixing in Solid-State Sodium Nano-Batteries (2023)

Nanostructured solid-state batteries (SSBs) are poised to meet the demands of next-generation energy storage technologies by realizing performance competitive to their liquid-based counterparts while simultaneously offering improved safety and expanded form factors. Atomic layer deposition (ALD) is among the tools essential to fabricate nanostructured devices with challenging aspect ratios. Here, we report the fabrication and electrochemical testing of the first nanoscale sodium all-solid-state battery (SSB) using ALD to deposit both the V<sub>2</sub>O<sub>5</sub> cathode and NaPON solid electrolyte followed by evaporation of a thin-film Na metal anode. NaPON exhibits remarkable stability against evaporated Na metal, showing no electrolyte breakdown or significant interphase formation in the voltage range of 0.05-6.0 V vs Na/Na<sup>+</sup>. Electrochemical analysis of the SSB suggests intermixing of the NaPON/V<sub>2</sub>O<sub>5</sub> layers during fabrication, which we investigate in three ways: <i>in situ</i> spectroscopic ellipsometry, time-resolved X-ray photoelectron spectroscopy (XPS) depth profiling, and cross-sectional cryo-scanning transmission electron microscopy (cryo-STEM) coupled with electron energy loss spectroscopy (EELS). We characterize the interfacial reaction during the ALD NaPON deposition on V<sub>2</sub>O<sub>5</sub> to be twofold: (1) reduction of V<sub>2</sub>O<sub>5</sub> to VO<sub>2</sub> and (2) Na<sup>+</sup> insertion into VO<sub>2</sub> to form Na<i><sub>x</sub></i>VO<sub>2</sub>. Despite the intermixing of NaPON-V<sub>2</sub>O<sub>5</sub>, we demonstrate that NaPON-coated V<sub>2</sub>O<sub>5</sub> electrodes display enhanced electrochemical cycling stability in liquid-electrolyte coin cells through the formation of a stable electrolyte interphase. In all-SSBs, the Na metal evaporation process is found to intensify the intermixing reaction, resulting in the irreversible formation of mixed interphases between discrete battery layers. Despite this graded composition, the SSB can operate for over 100 charge-discharge cycles at room temperature and represents the first demonstration of a functional thin-film solid-state sodium-ion battery.