gebhart/patrepeval huggingface dataset:
Src as a therapeutic target in anti-hormone/anti-growth factor-resistant breast cancer (2006)

Endocrine therapy is the treatment of choice in hormone receptor-positive breast cancer. However, the effectiveness of anti-hormone drugs, such as tamoxifen, is limited because of the development of resistance, ultimately leading to disease progression and patient mortality. Using in vitro cell models of anti-hormone resistance, we have previously demonstrated that altered growth factor signalling contributes to an endocrine insensitive phenotype. Significantly, our recent studies have revealed that the acquisition of endocrine resistance in breast cancer is accompanied by a greatly enhanced migratory and invasive phenotype. Furthermore, therapeutic intervention using anti-growth factor monotherapies, despite an initial growth suppressive phase, again results in the development of a resistant state and a further augmentation of their invasive phenotype. Using the dual specific Src/Abl kinase inhibitor, AZD0530, we have highlighted a central role for Src kinase in promoting the invasive phenotype that accompanies both anti-hormone and anti-growth factor resistance. Importantly, the use of Src inhibitors in combination with anti-growth factor therapies appears to be additive, producing a marked inhibitory effect on cell growth, migration and invasion and ultimately prevents the emergence of a resistant phenotype. These observations suggest that the inhibition of Src activity may present a novel therapeutic intervention strategy, particularly when used as an adjuvant in endocrine-resistant breast disease, with the potential to delay or prevent the acquisition of subsequent resistance to anti-growth factor therapies.
Supplementary Figures 1-7 from Capillary Isoelectric-Focusing Immunoassays to Study Dynamic Oncoprotein Phosphorylation and Drug Response to Targeted Therapies in Non–Small Cell Lung Cancer (2023)

&lt;p&gt;PDF - 611KB, Supplementary Figure S1. Phospho-ERK1/2 signal response to erlotinib treatment in mouse tissues; Supplementary Figure S2. Response in NSCLC cell to MEK inhibitor PD325901 treatment; Supplementary Figure S3. Quantitation of MEK signal response in NSCLC cells treated with MEK inhibitor PD325901; Supplementary Figure S4. MEK1/2 peak assignments determined using isoform and phospho-specific anti-MEK antibodies; Supplementary Figure S5. Cell viability assay of lung adenocarcinoma cells with erlotinib treatment; Supplementary Figure S6. MEK2 siRNA knockdown in HCC827 cells; Supplementary Figure S7. Signaling molecule responses in a lung carcinoma patient treated with erlotinib and AZD6244&lt;/p&gt;
General decapping activators target different subsets of inefficiently translated mRNAs (2018)

Abstract The Dcp1-Dcp2 decapping enzyme and the decapping activators Pat1, Dhh1, and Lsm1 regulate mRNA decapping, but their mechanistic integration is unknown. We analyzed the gene expression consequences of deleting PAT1, LSM1, or DHH1, or the DCP2 C-terminal domain, and found that: i) the Dcp2 C-terminal domain is an effector of both negative and positive regulation; ii) rather than being global activators of decapping, Pat1, Lsm1, and Dhh1 directly target specific subsets of yeast mRNAs and loss of the functions of each of these factors has substantial indirect consequences for genome-wide mRNA expression; and iii) transcripts targeted by Pat1, Lsm1, and Dhh1 exhibit only partial overlap, are generally translated inefficiently, and, as expected, are targeted to decapping-dependent decay. Our results define the roles of Pat1, Lsm1, and Dhh1 in decapping of general mRNAs and suggest that these factors may monitor mRNA translation and target unique features of individual mRNAs.
TIGRA — an architectural style for enterprise application integration (2001)

We report on experience that we made in the Trading room InteGRation Architecture project (TIGRA) at a large German bank. TIGRA developed a distributed system architecture for integrating different financial front-office trading systems with middle- and back-office applications. We generalize the experience by proposing an architectural style that can be re-used for similar enterprise application integration tasks. The TIGRA style is based on a separation of data representation using domain-specific XML languages from transport of those data with an appropriate middleware. We show how Markup languages, particularly the eXtensible Markup Language (XML) and eXtensible Stylesheet Language Transformations (XSLT), can be used to solve semantic data integration problems. We discuss that the strengths of middleware and markup languages are complementary and indicate the synergies yielded by deploying middleware and markup in the TIGRA style.
Small variable segments constitute a major type of diversity of bacterial genomes at the species level (2010)

This analysis uncovers the importance of this small-sized genome diversity, which we expect to be present in a wide range of bacteria, and possibly also in many eukaryotic genomes.
Energy from waste (1989)

As the municipal solid waste crisis worsens, waste-to-energy is becoming more attractive to electric utilities. Some have converted coal-burning power plants to burn refuse-derived fuel. Of a number of resource recovery technologies in various stages of development and use, two options are currently favored for converting waste to energy: mass burning, and processing MSW into refuse-derived fuel (RDF). The paper discusses TVA involvement in resource recovery facilities, and public concerns over the siting of such facilities.
Wheel wear and surface/subsurface qualities when precision grinding optical materials (2006)

An ultra precision large optics grinder, which will provide a rapid and economic solution for grinding large off-axis aspherical and free-form optical components, has been developed at Cranfield University. This paper presents representative grinding experiments performed on another machine - a 5 axes Edgetek - in order to verify the proposed BoX(r) grinding cycle. The optical materials assessed included; Zerodur(r), SIC and ULE(r), all three being materials are candidates for extreme large telescope (ELT) mirror segments. Investigated removal rates ranged from 2mm<sup>3</sup>/s to 200mm<sup>3</sup>/s. The higher removal rate ensures that a 1 metre size optic could be ground in less than 10 hours. These experiments point out the effect of diamond grit size on the surface quality and wheel wear. The power and forces for each material type at differing removal rates are presented, together with subsurface damage.
TFPIβ is the GPI-anchored TFPI isoform on human endothelial cells and placental microsomes (2011)

Tissue factor pathway inhibitor (TFPI) produces factor Xa-dependent feedback inhibition of factor VIIa/tissue factor-induced coagulation. Messages for 2 isoforms of TFPI have been identified. TFPIα mRNA encodes a protein with an acidic N-terminus, 3 Kunitz-type protease inhibitor domains and a basic C-terminus that has been purified from plasma and culture media. TFPIβ mRNA encodes a form in which the Kunitz-3 and C-terminal domains of TFPIα are replaced with an alternative C-terminus that directs the attachment of a glycosylphosphatidylinositol (GPI) anchor, but whether TFPIβ protein is actually expressed is not clear. Moreover, previous studies have suggested that the predominant form of TFPI released from cells by phosphatidylinositol-specific phospholipase C (PIPLC) treatment is TFPIα, implying it is bound at cell surfaces to a separate GPI-anchored coreceptor. Our studies show that the form of TFPI released by PIPLC treatment of cultured endothelial cells and placental microsomes is actually TFPIβ based on (1) migration on SDS-PAGE before and after deglycosylation, (2) the lack of a Kunitz-3 domain, and (3) it contains a GPI anchor. Immunoassays demonstrate that, although endothelial cells secrete TFPIα, greater than 95% of the TFPI released by PIPLC treatment from the surface of endothelial cells and from placental microsomes is TFPIβ.
Predicting Mechanical Properties of Material Extrusion Additive Manufacturing-Fabricated Structures with Limited Information: Harnessing Entanglement Theory and Modeling (2022)

Abstract Mechanical properties of additively manufactured structures fabricated using material extrusion additive manufacturing are predicted through combining thermal modeling with entanglement theory and molecular dynamics approaches. A one-dimensional model of heat transfer in a single road width wall is created and validated against both thermography and mechanical testing results. Various model modifications are investigated to determine which heat transfer considerations are important to predicting properties. This approach was able to predict tear energies on reasonable scales with minimal information about the polymer. Such an approach is likely to be applicable to a wide range of amorphous and low crystallinity thermoplastics.
Parallel Synthesis and Cytotoxicity Evaluation of a Polyamine−Quinone Conjugates Library (2008)

A library of 24 derivatives designed by combining two natural products-derived fragments was prepared and tested to determine their anticancer potential in HT29 colon cancer cells. All library members inhibit cell proliferation as measured by MTT mitochondrial functional assay, with IC50 values in the 1-100 microM range. Entry 1b caused apoptotic EGFR-mediated intracellular signaling. Thus, polyamino-quinones emerged as readily accessible and easily diversified scaffolds for anticancer lead discovery.
Modélisation du parallélisme dans les contours actifs. Application à la segmentation d'images OCT de la rétine et d'images de fond d'œil (2011)

National audience
Silicide-induced multi-wall carbon nanotube growth on silicon nanowires (2011)

A novel multi-walled carbon nanotube (MWNT) growth process is reported based on carbon incorporation in a nickel catalyst layer deposited via plasma-enhanced atomic layer deposition (PEALD) on silicon nanowires and silicon wafer substrates. As-deposited PEALD Ni films containing relatively high amounts of carbon (>18 at.%) were observed to promote the growth of MWNTs upon post-deposition rapid thermal annealing. For these films the carbon originated from the ALD precursor ligand and MWNT growth occurred in the absence of a vapor-phase carbon feedstock. MWNT growth relied on the formation of nickel silicide at the PEALD Ni/Si interface which increased the local carbon concentration in the Ni film sufficiently to promote carbon saturation/precipitation at Ni catalyst grains and nucleate MWNT growth. Similar MWNT growth from annealed PEALD Ni films was not observed on SiO(2)-coated Si wafer substrates, consistent with the role of silicidation in the observed Ni-catalyzed MWNT growth on Si. This MWNT growth mode requires neither the catalytic decomposition of a gaseous hydrocarbon source nor the high-temperature pyrolysis of metallocene materials and purposely avoids a catalyst diffusion barrier at the Si substrate, commonly used in MWNT growth processes on Si.
Upward Shift and Steepening of the Blood Pressure Response to Exercise in Hypertensive Subjects at High Altitude (2018)

URL: http://www.clinicaltrials.gov. Unique identifier: NCT01830530.
Biomimetic studies using artificial system. IV. Biomimetic peptide synthesis by using multi-functionalized crown ethers as a novel enzyme model. A new concepts in mimicking of enzyme-catalyzed bond-forming reactions. (1989)

A novel approach to the mimicking of enzyme-catalyzed bond-forming reactions has been examined using multifunctionalized chiral crown ethers. In addition to the 18-crown-6 moiety as a binding site, the host have one thiol and one thio ester with an N-protected alpha-amino acid or a peptide, and have successfully achieved peptide synthesis in an enzyme-mimetic reaction mode. This new method involves the following three key reactions. (1) Intra-complex thiolysis: the host carries out the rapid intra-complex thiolysis of alpha-amino acid ester salts to form the dithioester, corresponding to the assembly of two guests by the host. (2) Amide formation: intramolecular aminolysis occurs between the bound guests to form the amide bond. (3) Peptide chain elongation: as the thiol reactive group is regenerated, the above two reactions are repeated to elongate the peptide chain. Formal turnover of the enzyme model has been demonstrated by the synthesis of a tetrapeptide derivative by the repetition of the above processes.
Structural Studies and Anticancer Activity of a Novel Class of β‐Peptides (2014)

Functionalized oligomeric organic compounds with well-defined β-proline scaffold have been synthesized by a cycloadditive oligomerization approach in racemic and enantiopure forms. The structure of the novel β-peptides was investigated by NMR spectroscopic and X-ray methods determining the conformational shapes of the β-proline oligomers in solution and solid states. The main structural elements subject to conformational switches are β-peptide bonds between 5-arylpyrrolidine-2-carboxylic acid units existing in Z/E configurations. The whole library of short β-peptides and intermediate acrylamides has been tested on antiproliferative activity towards the hormone-refractory prostate cancer cell line PC-3 revealing several oligomeric compounds with low micromolar and submicromolar activities. Bromine-substituted dimeric and trimeric acrylamides induced caspase-dependent apoptosis of PC-3 cells through cell-cycle arrest and mitochondrial damage.
Role ofSalmonella entericaexposure in Chilean Crohn's disease patients (2013)

Our study found no association between exposure to SE and CD. We observed a positive correlation between SE exposure and cigarette smoking in Chilean patients with CD, but not with disease severity.
Genomic surveillance for multidrug-resistant or hypervirulent Klebsiella pneumoniae among United States bloodstream isolates (2022)

Examination of consecutive isolates from a single center demonstrated that multidrug-resistant high-risk clones are indeed common, but a small number of hypervirulent K. pneumoniae isolates were also observed in patients with no recent travel history to Asia, suggesting that these isolates are undergoing community spread in the United States. A larger collection of publicly available bloodstream isolate genomes also suggested that hypervirulent K. pneumoniae strains are present but rare in the USA; however, this collection appears to be heavily biased towards highly antibiotic-resistant isolates (and correspondingly away from hypervirulent isolates).
Effects of Cytotoxic Necrotizing Factor 1 and Lethal Toxin on Actin Cytoskeleton and VE-Cadherin Localization in Human Endothelial Cell Monolayers (1999)

Integrity of the vascular endothelium is largely dependent on endothelial cell shape and establishment of intercellular junctions. Certain pathogenic bacterial toxins alter the cytoskeletal architecture of intoxicated cells by modulating the GTPase activity of p21 Rho family proteins. In the present study we have analyzed the effect of Rho-directed toxins on the actin cytoskeleton and monolayer integrity of endothelial cells. We report here that Escherichia coli cytotoxic necrotizing factor 1 (CNF1) activates Rho in human umbilical vein endothelial cells (HUVEC). In confluent monolayers, CNF1 treatment induces prominent stress fiber formation without significantly modifying peripheral localization of VE-cadherin, a specific marker of vascular endothelial cell adherens junctions. Further, Rho activation with CNF1 blocks thrombin-induced redistribution of VE-cadherin staining and gap formation in HUVEC monolayers. Inhibition of Rho by prolonged treatment of cells with C3 exoenzyme (Clostridium botulinum) eliminates actin stress fibers without disrupting the continuity of VE-cadherin staining, indicating that Rho-dependent stress fibers are not required for maintaining this adhesion receptor at sites of intercellular contact. Lethal toxin (Clostridium sordellii), an inhibitor of Rac as well as Ras and Rap, potently disrupts the actin microfilament system and monolayer integrity in HUVEC cultures.
Signal Processing for Digital Beamforming FMCW SAR (2014)

According to the limitations of single channel Frequency Modulation Continuous Wave (FMCW) Synthetic Aperture Radar (SAR), Digital Beamforming (DBF) technology is introduced to improve system performance. Combined with multiple receive apertures, DBF FMCW SAR can obtain high resolution in low pulse repetition frequency, which can increase the processing gain and decrease the sampling frequency. The received signal model of DBF FMCW SAR is derived. The continuous antenna motion which is the main characteristic of FMCW SAR received signal is taken into account in the whole signal processing. The detailed imaging diagram of DBF FMCW SAR is given. A reference system is also demonstrated in the paper by comparing with a single channel FMCW SAR. The validity of the presented diagram is demonstrated with a point target simulation results.
3D ERT for the Study of an Ancient Wall Covered by Precious Mosaics (2009)

The DC electrical tomography (ERT) is still a method scarcely diffused for the study of masonry of recent and ancient buildings, in spite of the very high resolution power of this methodology. The major limitation on ERT applicability on precious surfaces (like masonries or floors with fresco or mosaic covering) depends on the method of current injection: usually it is necessary to drive electrodes (small nails) into small perforations on the surfaces, to obtain sufficiently high current values (to produce good signal to noise ratio). An interesting application of the MYG array (that reduces significantly, compared to other classical arrays, the number of electrodes utilized for current injection) has been carried out on a wall of the fountain room of the Zisa Palace (Palermo, XII century A.D.), covered by a mosaic wall suffering important detachment of tesserae due to internal moisture. The results seem to suggest that ERT investigation with the MYG array has important applicative potentiality in study of precious walls or floors in artistic buildings, because of the low-invasivity and high resolution power as well as velocity of acquisition.
Studieren, Lehren, Forschen — in Freiheit (2003)

In einer kompetitiven Welt autonomer Universitäten wird die Zufriedenheit der Studierenden, der Lehrer, Forscher und übrigen Mitarbeiter zum Erfolgsfaktor. Welche Erwartungen sind zu befriedigen? Wo müssen die Universitäten dazu eigene Initiativen über das Gesetz hinaus setzen? Auf einige besonders erfolgskritische Themen wird eingegangen: Konkurrenz als Motivationsfaktor; Strategie zur Schadensvermeidung; die Praxis indikatorgesteuerter Allokation innerhalb der Universität; Berufungspraxis; Mitbestimmung und Mitsprache; Personalentwicklung und Laufbahnbetreuung für Wissenschaftler.
A Comparison of Unicortical and Bicortical End Screw Attachment of Fracture Fixation Plates (1992)

Plate fixation is considered by many clinicians to be the treatment of choice for displaced diaphyseal fractures of the forearm. One possible complication associated with plate fixation is refracture with the plate in situ or after plate removal. With the plate in situ, refracture typically occurs through the last screw hole near the end of the plate. Some clinicians have advocated the use of unicortical end screws to minimize the risk of such refractures. In this study, we performed a series of in vitro tests to compare the breaking strength of plated bone analogues that used either unicortical or bicortical end screws. The plated constructs that used unicortical end screws were significantly weaker in the two most important physiologic loading modes. Based on these results, we conclude that the use of unicortical end screws may result in a greater risk of refracture with the plate in situ.
Downlink scheduling using non-orthogonal uplink beams (2014)

Opportunistic schedulers rely on the feedback of the channel state information of users in order to perform user selection and downlink scheduling. This feedback increases with the number of users, and can lead to inefficient use of network resources and scheduling delays. We tackle the problem of feedback design, and propose a novel class of nonorthogonal codes to feed back channel state information. Users with favorable channel conditions simultaneously transmit their channel state information via non-orthogonal beams to the base station. The proposed formulation allows the base station to identify the strong users via a simple correlation process. After deriving the minimum required code length and closed-form expressions for the feedback load and downlink capacity, we show that: the proposed algorithm reduces the feedback load while matching the achievable rate of full feedback algorithms operating over a noiseless feedback channel; and the proposed codes are superior to the Gaussian codes.
Characterization of Shigella flexneri in Northern Vietnam in 2012 –2016 (2022)

ntroduction: Shigellosis remains a considerable public health concern in developing countries. Shigella flexneri and Shigella sonnei are prevalent worldwide and S. sonnei has been replacing S. flexneri. Gap Statement: S. flexneri still causes outbreaks in Northern Vietnam and limited information is available on its genetic characteristics. Aim: This study aimed to characterize the genetic characteristics of S. flexneri strains from Northern Vietnam. Methodology: This study used 17 isolates from eight incidents, collected in Northern Vietnam between 2012 and 2016. The samples were subjected to whole genome sequencing (WGS), molecular serotyping, cluster analysis, and identification of antimicrobial resistance genes. Additionally, phylogenetic analysis was performed including isolates from previous studies. Results: Clusters were identified according to spatiotemporal backgrounds. The results suggested that two incidents in Yen Bai province in 2015 and 2016 were derived from a very recent common ancestor. All isolates belonged to phylogroup (PG) 3, which was divided into two sub-lineages. Thirteen of 17 isolates, including those from the Yen Bai incidents, belonged to sub-lineage Sub-1 and were serotyped as 1a. The remaining four isolates belonged to sub-lineage Sub-2 and were the globally predominant serotype 2a. The Sub-1 S. flexneri isolates possessed the gtrI gene, which encodes the glycosyl transferase that determines serotype 1a, with bacteriophage elements in the vicinity. Conclusion: This study revealed two PG3 sub-lineages of S. flexneri in Northern Vietnam, of which Sub-1 might be specific to the region.
Atomic Interactions in Stainless Austenitic CrMn Steels Alloyed with C, N or (C+N) (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.