gebhart/patrepeval huggingface dataset:
Dynamic feedback regulation for efficient membrane protein production using a small RNA-based genetic circuit in Escherichia coli (2022)

A dynamically regulated feedback circuit was developed that can sense metabolic stress caused by, in casu, the overexpression of an MP and responds to it by balancing the metabolic state of the cell and more specifically by downregulating the expression of the MP of interest. This negative feedback mechanism was established by implementing and optimizing simple-to-use genetic control elements based on post-transcriptional regulation: small non-coding RNAs. In addition to membrane-related stress when the MP accumulated in the cytoplasm as aggregates, the sRNA-based feedback control system was still effective for improving cell growth but resulted in a decreased total protein production. This result suggests promiscuity of the MP sensor for more than solely membrane stress.
Lower Level of Low Density Lipoprotein Cholesterol is Associated with a Higher Increase in the Fractional Flow Reserve in Patients with Fixed-dose Rosuvastatin (2017)

LDL cholesterol level was associated with the change in the FFR value in patients following stent implantation. Lower LDL cholesterol tended to improve in the long-term FFR, underscoring the importance of lowering LDL cholesterol to prevent the progression of coronary atherosclerosis.
Interaction of the Pseudomonas aeruginosa secretory products pyocyanin and pyochelin generates hydroxyl radical and causes synergistic damage to endothelial cells. Implications for Pseudomonas-associated tissue injury. (1992)

Pyocyanin, a secretory product of Pseudomonas aeruginosa, has the capacity to undergo redox cycling under aerobic conditions with resulting generation of superoxide and hydrogen peroxide. By using spin trapping techniques in conjunction with electron paramagnetic resonance spectrometry (EPR), superoxide was detected during the aerobic reduction of pyocyanin by NADH or porcine endothelial cells. No evidence of hydroxyl radical formation was detected. Chromium oxalate eliminated the EPR spectrum of the superoxide-derived spin adduct resulting from endothelial cell exposure to pyocyanin, suggesting superoxide formation close to the endothelial cell plasma membrane. We have previously reported that iron bound to the P. aeruginosa siderophore pyochelin (ferripyochelin) catalyzes the formation of hydroxyl free radical from superoxide and hydrogen peroxide via the Haber-Weiss reaction. In the present study, spin trap evidence of hydroxyl radical formation was detected when NADH and pyocyanin were allowed to react in the presence of ferripyochelin. Similarly, endothelial cell exposure to pyocyanin and ferripyochelin also resulted in hydroxyl radical production which appeared to occur in close proximity to the cell surface. As assessed by 51Cr release, endothelial cells which were treated with pyocyanin or ferripyochelin alone demonstrated minimal injury. However, endothelial cell exposure to the combination of pyochelin and pyocyanin resulted in 55% specific 51Cr release. Injury was not observed with the substitution of iron-free pyochelin and was diminished by the presence of catalase or dimethyl thiourea. These data suggest the possibility that the P. aeruginosa secretory products pyocyanin and pyochelin may act synergistically via the generation of hydroxyl radical to damage local tissues at sites of pseudomonas infection.
Adsorption and diffusion of Pt and Au on the stoichiometric and reducedTiO2rutile (110) surfaces (2005)

A comparative ab initio pseudopotential study of the adsorption and migration profiles of single neutral Pt and Au atoms on the stoichiometric and reduced $\mathrm{Ti}{\mathrm{O}}_{2}$ rutile (110) surfaces is presented. Pt and Au behave similarly with respect to (i) most favorable adsorption sites, (ii) the large increase in their binding energy when the surface is reduced, and (iii) their low migration barrier on the stoichiometric surface. Pt, on the other hand, binds more strongly (by $\ensuremath{\sim}2\phantom{\rule{0.3em}{0ex}}\mathrm{eV}$) to both surfaces. On the stoichiometric surface, Pt migration pattern is expected to be one dimensional, which is primarily influenced by interactions with O atoms. Au migration is expected to be two dimensional, with $\mathrm{Au}\ensuremath{-}\mathrm{Ti}$ interactions playing a more important role. On the reduced surface, the migration barrier of Pt trapped at an O vacancy site is significantly larger compared to that of Au.
EUV spectroscopy of tin-doped laser plasma source (2008)

Detailed spectroscopic studies on extreme ultra violet (EUV) emission from tin-doped droplet laser plasmas were completed using a combination of spectroscopic instruments that allow for quantitative spectroscopy throughout the radiation region of 5 nm to 550 nm.
Orientation dependence of higher order mode reflections in femtosecond pulse written fiber Bragg gratings (2012)

Ultrashort pulse lasers allow for the inscription of fiber Bragg gratings largely independent of the fiber geometry. Here, we investigate how the orientation of a reflected higher order mode depends on the FBG's cross-section.
A Recommendation Method Based on Semantic Similarity and Complementarity Using Weighted Taxonomy: A Case on Construction Materials Dataset (2018)

Products and web pages are the main components of the e-commerce data knowledge and the relationship among them is an important issue to be highly considered in recommender systems. This study aims to focus on the similarity and complementarity relationships among the products that have wide applications in the recommender systems. In the previously proposed methods, products and their relationships were revealed using taxonomy and “IS-A” relationship. In addition, the similarity and complementarity calculations were conducted based on edge computation by assigning a similar degree to any edge. More specifically, the children of a concept in the taxonomy was supported by a similar father’s “IS-A” degree. In contrast, this study provides a new approach based on ontology, data mining, and automatic discovering algorithms for the relationships with different degrees for the edges among the concepts. Accordingly, these relationships are initialised according to the “IS-A” degree. With regard to this weighted taxonomy, the semantic similarity and complementarity are measured based on concept distance. In addition, the proposed recommender system is item-based, which uses semantic similarity and complementarity. The required data for the present study were collected from construction materials supplier. The results illustrated that our proposed method is effective for construction materials recommendation.
Studies on the reaction of certain tomato genotypes and their F1 to combined infection by Meloidogyne incognita and Pseudomonas solana cearum (1992)

In a study to find out the reaction of certain tomato genotypes and their F1 hybrids to root-knot disease and bacterial wilt, it was found that the hybrids whose parents had dominant sources of resistance to bacterial wilt and root-knot diseases remained resistant to these diseases in the field. When the parents had partially dominant/recessive source of resistance to either of the diseases, the hybrids became susceptible to wilt. Still,itis possible to get F1 hybrids with desirable economic characters and combined resistance to bacterial wilt and root-knot disease.
Enzyme activities after in vitro and in vivo application of dichlorvos. (1985)

The pesticide dichlorvos inhibits not only cholinesterase but also alkaline phosphatase, lactate dehydrogenase and glutamate dehydrogenase competitively. A mixed type inhibition of glutamic-oxaloacetic transaminase is in contrast to the increased activity of glutamic-pyruvic transaminase after dichlorvos application. The activity of leucine aminopeptidase is not affected by the substance. After administering rats an acutely toxic dose of dichlorvos (70 mg per kg b.w.) in vitro-inhibitions other than that of cholinesterase could not be found.
P02-184 - The European Federation of Psychiatric Trainees’ Psychiatric Resident - Industry Relationship Survey (EFPT-PRIRS) (2010)

Introduction There is growing concern about the influence of the pharmaceutical industry on psychiatric teaching and psychiatric professionalism as a whole. As a consequence, several national and international medical and psychiatric associations have issued guidelines to regulate the interactions between physicians and industry. Objectives The EFPT-PRIRS study aims to provide the lacking data on the extent and nature of these interactions among psychiatric trainees across Europe. Methods Study objectives were determined by the EFPT research group (EFPT-RG), after discussion with national and international experts. A survey was then devised compiling previously published questionnaires extending them by questions with specific relevance to psychiatric trainees. The resulting questionnaire was piloted amongst members of the EFPT-RG, modified accordingly and subsequently distributed to the national study coordinators. All 24 EFPT member countries were invited to participate in the study and data collection is currently ongoing. Preliminary results Preliminary analysis reveals the vast differences in industry - trainee relationships across European countries as well as major differences in personal attitudes towards these interactions. EFPT-PRIRS will potentially have an impact on the regulation of the interactions between the pharmaceutical industry and psychiatric trainees.
α-Fluoromethyl Tryptophans via Imino Ene Reaction (2001)

A preparative simple synthesis of α-fluoromethyl tryptophans is described. The new method is based on a non-catalytic imine ene reaction of highly electrophilic imines derived from methyl bromodifluoro- and trifluoropyruvate, and 1-sulfonyl-3-methylene indolines.
The Inventory Management System using RFID: Requirements Management (2010)

This research was conducted to examine requirements management criteria needed in producing the inventory management system and to understand and take advantage of the opportunities of RFID afford as mechanism towards better inventory management. The goal is to improve the management of the conventional inventory system, by developing the computerized system with the involvement of RFID deploy-ment. Interviews, magazines, articles, journals, books and outline documents are collected throughout the gathering of requirements and thus provide vital information to the research. The materials used to enable the capturing the present of items are RFID tags attached to items and RFID reader connected to the computer. The techniques are created in order to capture the items being tagged and store the necessary informa-tion of the items into database via the system. The overall approach was to keep track the availability of the items inside the inventory. Upon completion of the research, it is found that the aspects identified in requirements management provide the basis in producing the inventory man-agement system and the product of the research is system prototype which is being demonstrated. Future works for this system are focus on the RFID utilization and establish the stability of RFID connection with the system so it becomes more efficient, more effective and consistent. Be-sides, it is recommended to broaden the scope, enhancing the existing functionalities, adding new valuable elements to the system and improve the attractiveness of the system interfaces. Keywords: RFID, Inventory Management System, Inventory System Criteria, Stock Management System, Fast Food Restaurant
PDCT-03. CLINICALLY INTEGRATED SEQUENCING IN THE MANAGEMENT OF CHILDREN WITH HIGH-RISK BRAIN TUMORS (2016)

In contrast to adult tumors, recent studies of pediatric tumors have shown that most harbor relatively few mutational events, many of which may be targetable with clinically available agents. We set out to evaluate the use of integrative clinical sequencing and genetic counseling in the management of pediatric patients with high-risk brain tumors. We performed an observational consecutive case-series involving 31 pediatric patients with brain tumors designated by treating neuro-oncologist to be high risk (>25% chance for treatment failure) (mean age 7.1 years, range age 0-17). Participants underwent integrative clinical exome (tumor and germline DNA) and transcriptome (tumor RNA) sequencing and genetic counseling. Results were discussed in a CNS precision medicine tumor board teleconferenced with clinicians at multiple children’s hospitals, which made recommendations. Targeted agents were recommended based on a system involving seven criteria, including pre-clinical/clinical data, CNS penetration data, and availability of the agent either through clinical trial or off-label use. Of the 31 enrolled patients, 25 (80.6%) had adequate tumor specimen for DNA/RNA sequencing [including 7 GBM/DIPGs, 8 medulloblastomas, 4 (16%) relapsed and 8 (32%) metastatic tumors]. Sequencing revealed actionable germline or somatic alterations in 12 (48%) tumors, of which 9 (36%) resulted in an impact on treatment, and 3 (12%) resulted in genetic counseling for germline finding. Seven of 11 patients (63.6%) with glial tumors were found to have an actionable result. Most targeted therapeutic interventions included use of tyrosine kinase inhibitors, HDAC inhibitors, or mTOR inhibitors. Two of 25 patients (8%) with therapeutic intervention achieved partial remission (8 months and 3 months/ongoing) and 6 (24%) had ongoing stable disease (1 to 10 months). In summary, clinically integrated sequencing resulted in alteration of management in 48% of high-risk pediatric brain tumor patients and was of particular relevance to those with glial tumors.
X-ray photoelectron spectroscopy of silica surfaces treated with polyfunctional silanes (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTX-ray photoelectron spectroscopy of silica surfaces treated with polyfunctional silanesKrishna M. R. Kallury, Ulrich J. Krull, and Michael. ThompsonCite this: Anal. Chem. 1988, 60, 2, 169–172Publication Date (Print):January 15, 1988Publication History Published online1 May 2002Published inissue 15 January 1988https://pubs.acs.org/doi/10.1021/ac00153a014https://doi.org/10.1021/ac00153a014research-articleACS PublicationsRequest reuse permissionsArticle Views807Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Huntington disease without CAG expansion: phenocopies or errors in assignment? (1994)

Huntington disease (HD) has been shown to be associated with an expanded CAG repeat within a novel gene on 4p16.3 (IT15). A total of 30 of 1,022 affected persons (2.9% of our cohort) did not have an expanded CAG in the disease range. The reasons for not observing expansion in affected individuals are important for determining the sensitivity of using repeat length both for diagnosis of affected patients and for predictive testing programs and may have biological relevance for the understanding of the molecular mechanism underlying HD. Here we show that the majority (18) of the individuals with normal sized alleles represent misdiagnosis, sample mix-up, or clerical error. The remaining 12 patients represent possible phenocopies for HD. In at least four cases, family studies of these phenocopies excluded 4p16.3 as the region responsible for the phenotype. Mutations in the HD gene that are other than CAG expansion have not been excluded for the remaining eight cases; however, in as many as seven of these persons, retrospective review of these patients' clinical features identified characteristics not typical for HD. This study shows that on rare occasions mutations in other, as-yet-undefined genes can present with a clinical phenotype very similar to that of HD.
Determination of Methemoglobin and Total Hemoglobin in Toxicological Studies by Derivative Spectrophotometry (2002)

A method for the determination of methemoglobin in the presence of other hemoglobin subforms (i.e., oxy-, deoxy-, and carboxyhemoglobin) by use of derivative spectrophotometry is proposed. The method, which uses the first-derivative of the spectrum at 645 nm, is straightforward and expeditious, so it is of special interest to forensic toxicology laboratories as it allows the simultaneous determination of the methemoglobin saturation percentage and the hemoglobin concentration. This facilitates interpretation of the results and provides a better understanding of the significance of methemoglobin saturation in specific cases. Based on an analysis of interferences, the presence of other hemoglobin subforms or of endogenous components of plasma does not detract in any way from the performance of the method.
Different sensitivity of neurons and neuroblastoma cells to quercetin treatment (2008)

Quercetin is a natural flavonoid with pro-apoptotic and antiproliferative properties. In this study, we determined the sensitivity of neurons and neuroblastoma cells on apoptosis and necrosis induction upon quercetin treatment. No expression of Hsp72 was observed in neurons, which were more sensitive to cell death upon quercetin treatment than neuroblastoma cells, where Hsp72 expression was observed. Reduction of Hsp72 gene expression in neuroblastoma cells by antisense oligonucleotides made them more sensitive to pro-apoptotic action of quercetin. Moreover, the flavonoid decreased Hsp27, procaspase-3, MRP and PKB expression in neuroblastoma cells and in neurons. Nuclear localization of mainly cytoplasmic Hsp27 was observed in neuroblastoma cells after treatment with high quercetin concentrations, while in neurons, the protein was present in nuclei both in control and quercetin treated cells. Our results suggest that quercetin induce apoptosis more effectively in cells with low level of Hsp 72 expression. Higher sensitivity of neurons for cell death after treatment with high quercetin concentrations in comparison to neuroblastoma cell line should also be taken into consideration in further studies on using studied flavonoid as therapeutic agent.
3624 Piston Effect (2006)

The piston effect means the uniform airflows are caused by the motion of trains in a tunnel. There are two processes the moving train give the momentum to the air in the tunnel, one is friction and the other is the collision. A variable to describe the collision is presented in terms of fluctuations of density and velocity of airflows in the annular region composed of the train and the tunnel wall. This is utilized to discuss the self-consistent equations describe the behavior of uniform airflows in the tunnel. The result of calculation has a good agreement with the data of experiment.
Compression Testing of Micro-Scale Unidirectional Polymer Matrix Composites (2015)

This project builds on work done by Lu et al. An experimental study is carried out to characterize the failure behavior of a fiber reinforced polymer matrix composite at the micro-scale using the same test methodology. In order to address the issue of catastrophic failure observed in the previous effort, a physical stop for the indenter that limits maximum displacement to a predetermined value is integrated into the specimen design. Micron-sized specimens of IM7/BMI unidirectional composite with an integrated indenter displacement control were fabricated using Focused Ion Beam (FIB) milling. The specimens were compression tested using a custom built, SEM-based in-situ micro-testing device. During compression, SEM images are acquired continuously between displacement intervals so the deformation phenomena can be observed. Initial results showed that the integrated indenter displacement control prevents complete destruction of the specimen after the onset of failure. Damage observed includes interface failure, broken fibers, and general crushing. Parallel efforts on larger-scale compressive testing are conducted on millimeter-sized specimens using an in situ mechanical test frame located in an X-ray micro computed tomography (μCT) system. Failure response includes longitudinal splitting or brooming and kinking. A quantitative comparison of the compressive strength and modulus obtained from the two size scales specimen shows that there is no indication of a size effect. The experimental results will be used to validate the numerical models of micro-compression behavior.
DCATT Dispersed Fringe Sensor (DFS) -- Modeling and Experiment with Transmissive Phase Plates (1999)

The alignment and phasing control of NGST's segment primary mirrors use images from the science camera instead of dedicated instruments.
Geometrical considerations for terrain mapping in SBR applications (2005)

Knowledge aided signal processing calls for the use of existing information such as digitized image maps to further suppress clutter in a typical radar detection operation. In the context of space based radar (SBR) applications, given the SBR parameters and the look angles the first problem is to translate these parameters into a data point on a map. This is realized by converting these parameters into conventional latitude and longitude and then using the corresponding image intensity to estimate the clutter covariance matrix. Using this approach, it is possible to pre-compute the desired weight vector for a location of interest on earth that the SBR is assigned to visit. As a result, using the measured data together with the weight vector, it is possible to test for target detection hypothesis accurately at the time of visit by the SBR.
Vibrational analysis of high-energy compounds: 1,3,3-trinitroazetidine and 1-acetyl-3, 3-dinitroazetidine (1999)

Abstract Infrared spectra were obtained and normal coordinate calculations were made for 1,3,3-trinitroazetidine (TNAZ) and N-acetyl-3,3-dinitroazetidine (ADNAZ) in an effort to learn more about the conformational behavior of these compounds and to make assignments of the infrared bands to the appropriate normal modes of vibration. Semi-empirical molecular orbital calculations were also made in order to obtain additional information about the molecular structures. Normal coordinate calculations were made first for ADNAZ. Appropriate force constants obtained for ADNAZ were then used as starting values for TNAZ. It was shown that the observed frequency shift of the C=O stretch band of ADNAZ and the NO2 antisymmetric stretch and NO2 out-of-plane wag bands of the N-NO2 group of TNAZ can be explained by a change from the conformation that is initially present to another conformation during recrystallization after mixtures are melted. However, these shifts may also be explained by intermolecular interactions that cause changes in the force constants of the bonds and angles that affect the frequencies of the three bands just mentioned.