gebhart/patrepeval huggingface dataset:
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease (2007)

The endosomal sorting complexes required for transport (ESCRTs) are required to sort integral membrane proteins into intralumenal vesicles of the multivesicular body (MVB). Mutations in the ESCRT-III subunit CHMP2B were recently associated with frontotemporal dementia and amyotrophic lateral sclerosis (ALS), neurodegenerative diseases characterized by abnormal ubiquitin-positive protein deposits in affected neurons. We show here that autophagic degradation is inhibited in cells depleted of ESCRT subunits and in cells expressing CHMP2B mutants, leading to accumulation of protein aggregates containing ubiquitinated proteins, p62 and Alfy. Moreover, we find that functional MVBs are required for clearance of TDP-43 (identified as the major ubiquitinated protein in ALS and frontotemporal lobar degeneration with ubiquitin deposits), and of expanded polyglutamine aggregates associated with Huntington's disease. Together, our data indicate that efficient autophagic degradation requires functional MVBs and provide a possible explanation to the observed neurodegenerative phenotype seen in patients with CHMP2B mutations.
Pulse stretching utilizing two-photon-induced light absorption (1970)

The effect of inserting an element exhibiting induced absorption into a <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Q</tex> -switched laser cavity is investigated theoretically and experimentally. The rate equations are solved assuming two types of nonlinear loss, one being proportional to the square of the laser intensity and the other being proportional to the product of laser intensity and density of excited electrons in the nonlinear absorber. Experiments are performed with a rotating-prism ruby laser with a CdS crystal in its cavity. It is established that two-photon absorption takes place, and as predicted by the theory that the output intensity and output energy both decrease and pulse length increases as compared with the normal <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Q</tex> -switched case. In addition, the output pulse has an oscillatory behavior, and it is suggested that this is caused by loss due to the excited electrons, which are found to have a lifetime of approximately 24 ns.
An Extension of the Hirshfeld Method to Open Shell Systems Using Fractional Occupations (2011)

In this work, a new partitioning method is presented which allows one to calculate properties of radicals, in particular, atomic spin populations. The method can be seen as an extension of the Hirshfeld-I method [ Bultinck , P. et al. J. Chem. Phys. 2007 , 126 , 144111 ], in which the atomic weight functions, defining the atoms-in-molecules, are constructed by means of an iterative scheme in which the charges of the atoms-in-molecules are altered but the spin remains fixed. The Hirshfeld-I method is therefore not suitable for the calculation of atomic spin populations of open-shell systems. The new fractional occupation Hirshfeld-I (FOHI) uses an iterative scheme in which both the atomic charge and spin are optimized, resulting in a self-consistent method for the calculation of atomic spin populations. The results obtained with the FOHI method are compared with experimental results obtained using polarized neutron diffraction, thus serving as a validation of the FOHI method as well as the Hirshfeld definition of atoms-in-molecules in general.
The Complete Amino Acid Sequence of Alanine Apolipoprotein (apoC-III), an Apolipoprotein from Human Plasma Very Low Density Lipoproteins (1974)

Abstract The alanine apolipoprotein (apoLp-Ala) was isolated from patients with familial hyperlipoproteinemia (type V). ApoLp-Ala is a single chain protein of 79 amino acids with a single carbohydrate side chain containing 1 mole each of galactosamine and galactose, and 0 to 2 moles of sialic acid per mole of protein. The complete amino acid sequence of apoLp-Ala was determined by manual and automated Edman degradations of the intact protein, and tryptic and succinylated tryptic peptides. The NH2-terminal residue is serine, the COOH-terminal residue alanine. The carbohydrate moiety is attached to threonine at position 74 in the sequence. The amino acid sequence of apoLp-Ala contains no long sequences of hydrophobic residues which contrasts with the linear amphipathic regions present in the two integral membrane proteins cytochrome b5 and glycophorin.
Novel and Efficient Synthesis of 4‐Substituted‐1,2,4‐triazolidine‐3,5‐diones from Anilines (2007)

A simple and efficient three‐step synthetic procedure for the preparation of 4‐substituted phenyl derivatives of 1,2,4‐triazolidindiones (urazoles), starting from anilines, has been developed. In this method, aniline derivatives were reacted with 4‐nitrophenyl chloroformate to provide corresponding carbamate derivatives. In the second step, semicarbazide derivatives were prepared from these carbamates by reaction with ethyl carbazate. The cyclization reaction of corresponding semicarbazides furnished 1,2,4‐triazolidindiones in high yields.
Small Adjustments, Big Effects: Physiologic Considerations for the Clinical Implementation of Next Generation Resuscitative Balloon Occlusion of the Aorta (REBOA) (2016)

Davidson, Anders J. MD; Ferencz, Sarah-Ashley E. MD; Russo, Rachel M. MD; Gotlieb, Oren; Neff, Lucas P. MD; Williams, Timothy K. MD; Johnson, Austin M MD Author Information
An extremely simple, convenient, and selective method for acetylating primary alcohols in the presence of secondary alcohols (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn extremely simple, convenient, and selective method for acetylating primary alcohols in the presence of secondary alcoholsKazuaki Ishihara, Hideki Kurihara, and Hisashi YamamotoCite this: J. Org. Chem. 1993, 58, 15, 3791–3793Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/jo00067a005https://doi.org/10.1021/jo00067a005research-articleACS PublicationsRequest reuse permissionsArticle Views6079Altmetric-Citations176LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (2007)

The mammary glands develop initially as buds arising from the ventral embryonic epidermis. Recent work has shed light on signaling pathways leading to the patterning and formation of the mammary placodes and buds in mouse embryos. Relatively little is known of the signaling pathways that initiate branching morphogenesis and the formation of the ducts from the embryonic buds. Previous studies have shown that parathyroid hormone-related protein (PTHrP; also known as parathyroid hormone-like peptide, Pthlh) is produced by mammary epithelial cells and acts on surrounding mesenchymal cells to promote their differentiation into a mammary-specific dense mesenchyme. As a result of PTHrP signaling, the mammary mesenchyme supports mammary epithelial cell fate, initiates ductal development and patterns the overlying nipple sheath. In this report, we demonstrate that PTHrP acts, in part, by sensitizing mesenchymal cells to BMP signaling. PTHrP upregulates BMP receptor 1A expression in the mammary mesenchyme, enabling it to respond to BMP4, which is expressed within mesenchymal cells underlying the ventral epidermis during mammary bud formation. We demonstrate that BMP signaling is important for outgrowth of normal mammary buds and that BMP4 can rescue outgrowth of PTHrP(-/-) mammary buds. In addition, the combination of PTHrP and BMP signaling is responsible for upregulating Msx2 gene expression within the mammary mesenchyme, and disruption of the Msx2 gene rescues the induction of hair follicles on the ventral surface of mice overexpressing PTHrP in keratinocytes (K14-PTHrP). Our data suggest that PTHrP signaling sensitizes the mammary mesenchyme to the actions of BMP4, triggering outgrowth of the mammary buds and inducing MSX2 expression, which, in turn, leads to lateral inhibition of hair follicle formation within the developing nipple sheath.
Organically Capped Silicon Nanoparticles with Blue Photoluminescence Prepared by Hydrosilylation Followed by Oxidation (2006)

A facile method of preparing stable blue-emitting silicon nanoparticles that are dispersible in common organic solvents is presented. Oxidation of yellow-emitting silicon nanoparticles with an organic monolayer grafted to their surface, using either UV irradiation in solution or heating in air, converted them to blue-emitting particles. The evolution of the PL spectrum and infrared absorption spectrum of the particles was followed during the oxidation process. The PL spectrum showed a decrease in the PL emission peak near 600 nm and the appearance and increase in intensity of a PL emission peak near 460 nm rather than a smooth blue shift of the emission spectrum from yellow to blue. The organic monolayer grafted to the particle surface was not degraded by this oxidation process, as demonstrated by FTIR and NMR spectroscopy. Similar results were achieved for particles with styrene, 1-octene, 1-dodecene, and 1-octadecene grafted to their surface, demonstrating that it is the silicon nanocrystal, and not the organic component, that is essential to this process. The organic monolayer allows the nanoparticles to form stable, clear colloidal dispersions in organic solvents and provides for the possibility of further chemical functionalization of the particles. Combined with previous work on organically grafted silicon nanoparticles with green through near-infrared emission, this enables the efficient and scalable preparation of stable colloidal dispersions of organically grafted silicon nanoparticles with emission spanning the entire visible spectrum.
Synergistic effect of fruit–seed mixed juice on inhibition of angiotensin I-converting enzyme and activation of NO production in EA.hy926 cells (2018)

Commonly consumed fruit juices possess low inhibitory activity of angiotensin I-converting enzyme (ACE), which plays central role in elevation of blood pressure. The ACE inhibitory activity of fruit-seed mixed juice may be improved via synergistic interactions. In this study, the investigated synergistic, additive, and antagonistic effects of fruit-seed combination on ACE inhibition were investigated. Thirteen fruits and 15 seeds including legumes, nuts, and cereals were combined in pairs; pear-hemp seed-pumpkin seed juice (3-mixed juice) displayed the highest ACE inhibition resulting from synergistic interactions. Additionally, nitric oxide production in human endothelial cells was promoted by 3-mixed juice. Three-mixed juice showed antioxidant activities such as DNA protective, DPPH radical scavenging, and reducing effects. These results suggested that combinations of different food categories are beneficial for improving biological functions such as vascular health. Three-mixed juice, which shows high ACE inhibitory activity, may be useful as an anti-hypertensive agent and for treating hypertension.
Efficacy of Oxymorphone Extended Release in Postsurgical Pain: A Randomized Clinical Trial in Knee Arthroplasty (2004)

Patients with moderate or severe pain following knee arthroplasty and washout from standard patient-controlled analgesia (PCA) were randomized to receive 20 mg of an extended-release (ER) oxymorphone formulation (n = 65) or placebo (n = 61) q12h for 1 day. Oxymorphone PCA was used as rescue analgesic. Oxymorphone ER provided significant improvements over placebo for most standard single-dose analgesic parameters, including mean total pain relief (TOTPAR) over 0 to 12 hours (19.30 vs. 13.72; p = 0.0056), as well as for all multiple-dose (24-h) efficacy assessments. Oxymorphone-treated patients used significantly less rescue PCA than those who received placebo (p < 0.02). Adverse events such as nausea and constipation were typical of opioids, and laboratory and physical findings were similar between groups. Oxymorphone ER was effective and generally well tolerated. A single dose was active from 2 hours until > or = 12 hours after administration. Comparisons with other oral opioids are warranted, especially in the setting of outpatient and day surgery.
Gene expression profile of mouse masseter muscle after repetitive electrical stimulation (2009)

Dramatic gene expression changes were induced by the repetitive electrical muscle stimulation in mouse masseter.
Supplementary Tables S1-S3 from <i>LZTR1</i> Mutation Mediates Oncogenesis through Stabilization of EGFR and AXL (2023)

&lt;p&gt;Table S1. List of ubiquitylated peptides from DiGly proteomics assay. Table S2. List of proteins differentially abundant in LZTR1-/- compared toLZTR1+/+ cells quantified by TMT.Table S3. List of top scoring LZTR1 interacting proteins from immunoprecipitation/mass spectrometry.&lt;/p&gt;
The future is here: ventricular assist devices for the failing heart (2009)

Mechanical circulatory support is an important adjunct to the management of patients with advanced heart failure. Technological advances in this area have improved overall survival and decreased the incidence of complications. In addition, they have expanded the population suitable for this therapy. The challenge for clinicians is to translate the clinical evidence into the selection of the most appropriate device that will benefit an individual patient. This paper will review ventricular assist devices currently available and their clinical indications.
IL-10 transcription is negatively regulated by BAF180, a component of the SWI/SNF chromatin remodeling enzyme (2012)

These results demonstrate that BAF180 is a repressor of IL-10 transcription in Th2 cells and suggest that the differential recruitment of different SWI/SNF subtypes can have direct consequences on chromatin structure and gene transcription.
Effects of some factors on magnetic properties of non-oriented silicon steel sheet (1998)

The property of core loss of the non-oriented silicon steel sheets as a function of thickness, silicon contents and grain size were measured by SST(Single Sheet Tester). The specimens were excited by sinusoidal wave, AC(50Hz) with a single high order harmonic component and PWM waveforms. Si contents influence scarcely the core losses of the specimen with 0.35mm thickness independent of excitation waveforms when the grain size was large. But, in case of the core losses of the specimen with 0.50mm thickness, Si contents influence on under PWM waveforms excitation and under ACSH waveforms excitation. It is supposed that the effects of high order harmonic component originated in non-sinusoidal excitation largely appear in the core loss on condition that the specimen is thicker, Si contents is lower and grain size is larger and that is mainly caused by the eddy current loss.
Readout process analysis of super-RENS disk (2004)

We describe the unique characteristics of optical readout signals, and discuss the super-resolution mechanism in Platinum oxide super-RENS disks.
Visualization of Plasmonic Couplings Using Ultrafast Electron Microscopy (2021)

Hybrids of graphene and metal plasmonic nanostructures are promising building blocks for applications in optoelectronics, surface-enhanced scattering, biosensing, and quantum information. An understanding of the coupling mechanism in these hybrid systems is of vital importance to its applications. Previous efforts in this field mainly focused on spectroscopic studies of strong coupling within the hybrids with no spatial resolution. Here we report direct imaging of the local plasmonic coupling between single Au nanocapsules and graphene step edges at the nanometer scale by photon-induced near-field electron microscopy in an ultrafast electron microscope for the first time. The proximity of a step in the graphene to the nanocapsule causes asymmetric surface charge density at the ends of the nanocapsules. Computational electromagnetic simulations confirm the experimental observations. The results reported here indicate that this hybrid system could be used to manipulate the localized electromagnetic field on the nanoscale, enabling promising future plasmonic devices.
Supplemental Figure S5 from Reduction of MDSCs with All-trans Retinoic Acid Improves CAR Therapy Efficacy for Sarcomas (2023)

&lt;p&gt;ATRA treatment of 143b osteosarcoma cells does not change GD2 expression.&lt;/p&gt;
Inconsistencies over time in 5% of NetAffx probe-to-gene annotations (2005)

We recommend to report probe set identifiers when publishing microarray results, and to submit those analyses to microarray public databases to ensure that the interpretation of the data is updated with the latest set of annotations.
User model elicitation and enrichment for context-sensitive personalization in a multiplatform tv environment (2009)

With the rising integration of more and more online data sets with various hardware platforms, such as set-top boxes, mobile phones, it becomes increasingly crucial to have intelligent systems guiding the user through the maze of data. A necessity in such systems is a comprehensive user model which allows the system to know how and what the user might expect. In iFanzy, a context-sensitive personalized TV guide, we amass such a user model by monitoring the user's actions and behavior and extensively making use of Semantic Web techniques to interpret these activities and offer in the right moment the right content to the user. We focus on solving the 'cold start' problem by importing user data from external to iFanzy online applications. This is realized by exploiting background ontological knowledge to map the imported concepts, so that iFanzy can minimize the effort in accumulating a complete user model.
A ratiometric fluorescent probe for specific detection of cysteine over homocysteine and glutathione based on the drastic distinction in the kinetic profiles (2011)

The first ratiometric fluorescent probe for specific detection of cysteine over homocysteine and glutathione was developed based on the drastic distinction in the kinetic profiles.
Characterization of Lateral Junctions and Micro-SQUIDs Involving Magnetic Multilayers (2023)

We have fabricated and characterized at low temperatures Nb/Ni/(Al/Ni) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">14</sub> /Nb lateral junctions and Nb micro-SQUIDs involving (Al/Py) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">10</sub> multilayers (here Py is permalloy: 80% Ni − 20% Fe). We have studied <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I</i> - <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">V</i> curves and Josephson critical current as a function of an external magnetic field ( <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> ( <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">H</i> )) at various temperatures and orientations of the applied magnetic field. These studies revealed that, for some orientations of the externally applied magnetic field, the <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> ( <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">H</i> ) dependence of the lateral junctions has a component highly sensitive to magnetic field. In addition, we have observed a SQUID-like <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> ( <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">H</i> ) dependence for devices, in which an orthogonally oriented (with respect to the substrate) Nb loop that includes two nanoscopic Josephson junctions is filled with a (Al/Py) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">10</sub> multilayer. We believe the devices presented here are promising as magnetic field sensors on nanoscale for various applications where high spatial resolution is required.
Inhaled budesonide inhibits OVA-induced airway narrowing, inflammation, and cys-LT synthesis in BN rats (2000)

The objective of the present investigation was to examine the effects of an inhaled glucocorticoid, budesonide, on antigen-induced production of cysteinyl leukotrienes (cys-LTs) and pulmonary inflammatory cell infiltration in the Brown Norway rat, an animal model of asthma. Two weeks after sensitization to ovalbumin, rats were treated with budesonide (2.5 mg/kg) 18 and 1 h before challenge with antigen. Budesonide abolished the late response to ovalbumin (P<0.02) and strongly inhibited the in vivo synthesis of N-acetyl-leukotriene E(4), an indicator of cys-LT synthesis, during this period (P<0.005). Both total bronchoalveolar lavage (BAL) cells (P<0.01) and BAL macrophages (P<0.005) were markedly reduced to approximately 25% of their control levels after treatment with budesonide. It can be concluded that inhibition of the antigen-induced late response in Brown Norway rats by budesonide is associated with reductions in both BAL macrophages and cys-LT synthesis. It is possible that the effect of budesonide on cys-LT synthesis is related to its effects on pulmonary macrophages.
Design Issues in the Use of Robots as Cognitive Enhancement Aids (1988)

Abstract Robotic manipulators are being used in an investigation of their potential as effective classroom learning aids when used by severely orthopaedically disabled children to manipulate educational materials and to play games. During the course of the design and development of a prototype robotic system for educational use, several issues have surfaced that constitute serious considerations in the process. Of particular importance are issues involving accessibility, software design, curriculum integration and specific research questions involving spatial visualisation. Performance data from previous investigations has shown that learning to use robots can be easily accomplished, and that the consequent learning is remarkably resistant to decay over time.