gebhart/patrepeval huggingface dataset:
Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule (2023)

Of late, numerous microphysiological systems have been employed to model the renal proximal tubule. Yet there is lack of research on refining the functions of the proximal tubule epithelial layer-selective filtration and reabsorption. In this report, pseudo proximal tubule cells extracted from human-induced pluripotent stem cell-derived kidney organoids are combined and cultured with immortalized proximal tubule cells. It is shown that the cocultured tissue is an impervious epithelium that offers improved levels of certain transporters, extracellular matrix proteins collagen and laminin, and superior glucose transport and P-glycoprotein activity. mRNA expression levels higher than those obtained from each cell type were detected, suggesting an anomalous synergistic crosstalk between the two. Alongside, the improvements in morphological characteristics and performance of the immortalized proximal tubule tissue layer exposed, upon maturation, to human umbilical vein endothelial cells are thoroughly quantified and compared. Glucose and albumin reabsorption, as well as xenobiotic efflux rates through P-glycoprotein were all improved. The data presented abreast highlight the advantages of the cocultured epithelial layer and the non-iPSC-based bilayer. The in vitro models presented herein can be helpful in personalized nephrotoxicity studies.
Refractive Structure-from-Motion on Underwater Images (2013)

In underwater environments, cameras need to be confined in an underwater housing, viewing the scene through a piece of glass. In case of flat port underwater housings, light rays entering the camera housing are refracted twice, due to different medium densities of water, glass, and air. This causes the usually linear rays of light to bend and the commonly used pinhole camera model to be invalid. When using the pinhole camera model without explicitly modeling refraction in Structure-from-Motion (SfM) methods, a systematic model error occurs. Therefore, in this paper, we propose a system for computing camera path and 3D points with explicit incorporation of refraction using new methods for pose estimation. Additionally, a new error function is introduced for non-linear optimization, especially bundle adjustment. The proposed method allows to increase reconstruction accuracy and is evaluated in a set of experiments, where the proposed method's performance is compared to SfM with the perspective camera model.
Selective site controlled nucleophilic attacks in 5-membered ring phosphate esters: unusual C–O vs. common P–O bond cleavage (2005)

Clean endocyclic C-O bond cleavage has been achieved in the reactions of 5-membered phosphate triesters with various nucleophiles.
Second-derivative infrared spectroscopic studies of the secondary structures of bacteriorhodopsin and calcium-ATPase (1985)

The resolution of minor amide components in the infrared spectra of membrane proteins has, in the past, been limited by the small differences in frequency compared to the large half-widths of the bands that are assigned to different secondary conformations. Here, second-derivative calculations are used to resolve the relatively weak bands that are associated with the beta-sheet conformation and the vibrations of some amino acid side chains in the infrared spectra of bacteriorhodopsin and Ca2+-activated adenosine-5'-triphosphatase (Ca2+-ATPase). The spectra presented indicate that bacteriorhodopsin in the purple membrane contains an appreciable amount of beta structure in addition to the predominant alpha II-helical structure. Both sarcoplasmic reticulum and purified Ca2+-ATPase in native lipids contain alpha-helical and random coil conformations together with a small amount of beta structure. In 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) Ca2+-ATPase adopts a secondary conformation similar to that in the sarcoplasmic reticulum, and this structure is unaffected by the phospholipid phase transition. A shift to a predominantly random coil conformation is associated with solubilization of both bacteriorhodopsin and Ca2+-ATPase in 20% Triton X-100. Second-derivative analysis of the carbonyl stretching vibrations of DMPC bilayers indicates that below the phase-transition temperature (Tm) both bacteriorhodopsin and Ca2+-ATPase perturb the interface region such that the sn-2 carbonyls adopt a conformation similar to the sn-1 carbonyls. Above Tm, these integral proteins have no effect on the static order of the interface region, and the conformational inequivalence of the sn-1 and sn-2 carbonyls is similar to that found in a pure lipid bilayer.
Near-ultraviolet excitable orange-yellow Sr3(Al2O5)Cl2:Eu2+ phosphor for potential application in light-emitting diodes (2008)

Sr 3 ( Al 2 O 5 ) Cl 2 phosphor doped with Eu2+ was prepared by a soli-state reaction. This phosphor emits a broad orange-yellow luminescence with a peak wavelength of 620nm and a full width at half maximum of about 175nm under near-ultraviolet (NUV) excitation at ∼400nm. Yellow light-emitting diodes (LEDs) for general lighting were fabricated by combining Sr3(Al2O5)Cl2:Eu2+ phosphor with an NUV chip. The phosphor-converted LEDs had a color temperature of about 2300K and their color rendering index was 74.
Lack of a site-specific phosphorylation of Presenilin 1 disrupts microglial gene networks and progenitors during development (2020)

Microglial cells play a key role in brain homeostasis from development to adulthood. Here we show the involvement of a site-specific phosphorylation of Presenilin 1 (PS1) in microglial development. Profiles of microglia-specific transcripts in different temporal stages of development, combined with multiple systematic transcriptomic analysis and quantitative determination of microglia progenitors, indicate that the phosphorylation of PS1 at serine 367 is involved in the temporal dynamics of microglial development, specifically in the developing brain rudiment during embryonic microgliogenesis. We constructed a developing brain-specific microglial network to identify transcription factors linked to PS1 during development. Our data showed that PS1 functional connections appear through interaction hubs at Pu.1, Irf8 and Rela-p65 transcription factors. Finally, we showed that the total number of microglia progenitors was markedly reduced in the developing brain rudiment of embryos lacking PS1 phosphorylation compared to WT. Our work identifies a novel role for PS1 in microglial development.
Acoustic neuromas (Diagnostic value of testing the function of the trigeminal nerve, the cerebellum and optokinetic nystagmus (1983)

The involvement of the trigeminal nerve, cerebellum, and optokinetic nystagmus in patients with acoustic neuromas, as well as the methods of investigation, are described. The corneal and/or facial sensibility was found to be reduced in 29 per cent of the whole series and in 53 per cent of tumors larger than 40 mm. There was a significant correlation between reduced corneal and/or facial sensibility and the findings of pressure at the trigeminal root at operation. Only three patients had a persistent reduction of trigeminal function post-operatively. Cerebellar dysfunction was found in 32 per cent, but significantly more frequently (58 per cent) in patients with tumors larger than 40 mm. Post-operatively, six patients had cerebellar symptoms in the form of gait disturbances; five of these patients had a supplementary suboccipital removal performed, after the initial translabyrinthine approach. A defective optokinetic nystagmus was found pre-operatively in 10 patients, nine of whom had tumors larger than 40 mm in diameter. All patients with a defective optokinetic nystagmus had a large anatomic impression in the pons at operation. In patients suspected of having an acoustic neuroma, symptoms from the trigeminal nerve, the cerebellum and the optokinetic nystagmus predict the presence of a large tumor and subsequent difficulties at operation. The symptoms were completely reversible in the vast majority of cases and post-operative symptoms persisted only in patients in whom tumor removal was difficult and the tumor very large. Testing of the trigeminal nerve, the cerebellum and the optokinetic nystagmus still deserves its place in the diagnostic work-up of patients with unilateral acoustic or vestibular symptoms, especially in cases with severe hearing impairment, which necessitate the use of tests that are independent of acoustic function.
Comparison of parental and health care professional preferences for the acellular or whole cell pertussis vaccine (1998)

Mothers prefer a less reactogenic vaccine product even if it requires multiple injections. Health care professionals are more concerned about multiple injections and are poor predictors of mothers' vaccine preference. Multiple injections may be more a barrier to immunization for health care professionals than for mothers.
Letter to the Editor (2006)

Dear Dr. Deutsch, I am writing to congratulate you, the editorial board members, reviewers, and authors of the Journal of Neurological Physical Therapy for the journal's recent indexing on Medline and other innovations that the journal has implemented. Your recent editor's note speaks to the importance of Knowledge Translation (KT). The indexing of the journal on Medline greatly enhances KT through the wider audience the journal has gained. I was an author on an article that was published in the journal in the March 2005 issue, prior to it being indexed on Medline. Since January of this year, after the journal was indexed on Medline, I have had over 10 requests for reprints or e-mails asking about the paper, 3 of these were from Europe and one from the Middle East. It has been exciting corresponding with other researchers and clinicians who may have not been aware of this article if not for the journal's indexing on Medline. Congratulations, George Fulk, PT, PhD Physical Therapy Program, Clarkson University
РАЗВИТИЕ ПАРКА ГИБРИДНЫХ АВТОМОБИЛЕЙ (2015)

The comprehensive review of the development of hybrid cars production from the origins through the year 2012 shows significant differences between various countries and regions over the world as for the levels of manufacturing and sales.
Development of rehabilitation engineering over the years: as I see it. (2007)

The descriptive words “Rehabilitation Engineering” were not used widely, if at all, in America until the late 1960s and early 1970s, and then mostly by persons who had first been involved with prosthetics research and development immediately after World War II. Rehabilitation engineering in America owes its birth to the federal agencies that fostered its development after they had been so successful in supporting programs of research, education, and development in limb prosthetics and orthotics (initially for veterans) during the period from 1945 to 1970. But before its more or less formal birth in America around 1970, examples of engineering activities in the rehabilitation field can be cited through much of recorded history.
Immunity as a Factor in the Epidemiology of Medieval Plague (1984)

The rise, disappearance, and demography of medieval plague remain mysterious. This paper reviews those features of plague immunity in animals and humans that might help explain these aspects of medieval plague. The absence of a focus of sylvatic plague in medieval Europe is postulated, while it is suggested that the susceptible population was much more varied both in terms of immunity and number of species than has been assumed. The sex, age, and regional variations in incidence of plague among humans are examined on the basis of primary and secondary historical sources. Nutritional factors, especially iron status, and cross-immunities with other diseases help provide an explanation of the observations in the literature.
Mycotoxin production by Chaetomium spp. and related fungi (1981)

Screening for mycotoxin production by Chaetomium spp. and related fungi on rice culture was conducted by a combination of cytotoxicity tests using HeLa cells and thin-layer chromatography. Producers of sterigmatocystin, O-methylsterigmatocystin, chaetochromin, chaetocin, chetomin, cochliodinols, and mollicellin G were found and the taxonomic significance of these findings is discussed.
Blood Concentration by Superposition of Higher Harmonics of Ultrasound (1997)

A method for concentrating cells in blood using an acoustic radiation force generated by superposition of the higher harmonics on fundamental ultrasound has been investigated. From the theoretical estimation, the efficiency of concentration of blood cells in a chamber based on a rectangular acoustic radiation force slope calculated by integration of the higher harmonics of ultrasound was 17% better than that based on a sine wave, while the peak pressure was reduced to 82% of that in the sine wave case. Concentration of blood cells was also performed experimentally using superposition of higher harmonics on a 500 kHz sine wave. At a 1.2 m l /min fluid flow rate, the blood cells were concentrated on the pressure node within 9 s, with a cell distribution half-width of 348 µ m (i.e., 76% of blood cells concentrated within 23% of the chamber width), which is 5% narrower than that of the sine wave. Though the experimental result was insufficient for the realization of the theoretical estimation because of the incomplete synthesis of the desired waveform, these results suggest the potential usefulness of this superposition method for improving the concentration efficiency with maintenance of the width of the potential slope and reduction of peak pressure.
secHsp70 as a tool to approach amyloid-β42 and other extracellular amyloids (2017)

Self-association of amyloidogenic proteins is the main pathological trigger in a wide variety of neurodegenerative disorders. These aggregates are deposited inside or outside the cell due to hereditary mutations, environmental exposures or even normal aging. Cumulative evidence indicates that the heat shock chaperone Hsp70 possesses robust neuroprotection against various intracellular amyloids in Drosophila and mouse models. However, its protective role against extracellular amyloids was largely unknown as its presence outside the cells is very limited. Our recent manuscript in PNAS revealed that an engineered form of secreted Hsp70 (secHsp70) is highly protective against toxicity induced by extracellular deposition of the amyloid-β42 (Aβ42) peptide. In this Extra View article, we extend our analysis to other members of the heat shock protein family. We created PhiC31-based transgenic lines for human Hsp27, Hsp40, Hsp60 and Hsp70 and compared their activities in parallel against extracellular Aβ42. Strikingly, only secreted Hsp70 exhibits robust protection against Aβ42-triggered toxicity in the extracellular milieu. These observations indicate that the ability of secHsp70 to suppress Aβ42 insults is quite unique and suggest that targeted secretion of Hsp70 may represent a new therapeutic approach against Aβ42 and other extracellular amyloids. The potential applications of this engineered chaperone are discussed.
Magnesium Alloys Implants Coated with 58S Sol-Gel Bioactive Glass to Retard First Stage Corrosion (2017)

Two compositions of 58S (60 mol% SiO2, 36 mol% CaO, 4 mol% P2O5) glass coatings were made by sol-gel and applied by dip coating onto two commercial magnesium alloys (AZ31 and AZ91) as a strategy to retard the hydrogen evolution and accelerate the deposition of a hydroxyapatite layer to enhance bioactivity. Surface studies with x-ray photoelectron spectroscopy, Raman spectroscopy, and scanning electronic microscopy techniques are presented after coating deposition and after immersion in Hanks’ balanced salt solution (HBSS) at 37°C. Electrochemical tests were also conducted to evaluate the evolution of the coating with immersion. It is observed that even though the coating presents defects, it is able to retard substrate degradation, and this effect is more pronounced for AZ91 as substrate. Coating performance is mainly governed by the substrate because of defective nature of the film.
Effect of age and pregnancy status on adaptive immune responses of Canadian Holstein replacement heifers (2011)

Selection for production traits with little or no emphasis on health traits has led to an increase in the incidence of disease in Canadian dairy herds. We describe here a patented protocol for estimating the breeding value for immune responsiveness in heifers that combines measures of both cell-mediated (CM) and antibody-mediated (AM) immune responses (IR). The ability of putative type 1 and type 2 antigens used to induce CMIR and AMIR, respectively, was assessed in replacement Holstein heifers, and the effects of age and pregnancy on type 1 and type 2 IR bias were estimated. Results demonstrated that the type 1 and type 2 antigens induced polarized type 1 and type 2 responses in heifers regardless of age and pregnancy status, and can therefore be used to identify animals with superior overall immune responsiveness. However, age and pregnancy status had significant effects on adaptive IR profiles, highlighting the need for appropriate statistical modeling of such effects when ranking animals on their ability to mount CM and AMIR. Responses became increasingly type 1 biased as heifers approached 12 mo of age, from which point, responses then became increasingly type 2 biased with age and length of gestation. Knowledge of how age and pregnancy influence the dynamics of type 1 and type 2 IR bias is expected to improve our ability to select animals with enhanced immune responsiveness and aid in the development of effective vaccines through strategic targeting of vaccine components to recipients.
Spontaneous radiation from relativistic electrons in a taper undulator. Technical report (1983)

Collecting duct carcinoma is associated with poor prognosis. For the majority of patients surgical treatment will not result in a cure. Previously recommended chemotherapy and/or immunotherapy appears to have a limited role in treatment of this disease, and early detection may be the best method for prolonging patient survival.
Possible Transfusion-Transmitted Babesia divergens–like/MO-1 Infection in an Arkansas Patient (2017)

A patient with asplenia and multiple red blood cell transfusions acquired babesiosis infection with Babesia divergens-like/MO-1 organisms and not Babesia microti, the common United States species. He had no known tick exposure. This is believed to be the first transfusion-transmitted case and the fifth documented case of B. divergens-like/MO-1 infection.
Internet como espacio de juego: del game a la gamificación (2015)

La revista francófona Sciences du jeu.org acaba de publicar su segundo número, dedicado a los conceptos de gamificación, ludificación y ludicización (“Questionner les mises en forme ludiques du web : gamification, ludification et ludicisation”). En la introducción, Maude Bonenfant (Universidad de Quebec, Montreal) y Sébastien Genvo (Universidad de Lorraine) explican que la propagación y la multiplicación del juego digital en diferentes sectores de actividad ha generado diferentes conceptos y marcos teóricos destinados a describir y analizar dicho fenómeno. El concepto de gamificación se ha expandido rápidamente en la investigación académica anglosajona, el marketing y el diseño de juegos
Virulence Effect of Enterococcus faecalis Protease Genes and the Quorum-Sensing Locus fsr in Caenorhabditis elegans and Mice (2002)

The expression of two Enterococcus faecalis extracellular virulence-related proteins, gelatinase (GelE) and serine protease (SprE), has been shown to be positively regulated by the fsr quorum-sensing system. We recently developed a novel system for studying E. faecalis pathogenicity that involves killing of the nematode worm Caenorhabditis elegans and showed that an E. faecalis fsrB mutant (strain TX5266) exhibited attenuated killing. We explore here the role of the fsr/gelE-sprE locus in pathogenicity by comparing results obtained in the nematode system with a mouse peritonitis model of E. faecalis infection. Insertion mutants of fsrA (TX5240) and fsrC (TX5242), like fsrB (TX5266), were attenuated in their ability to kill C. elegans. A deletion mutant of gelE (TX5264) and an insertion mutant of sprE (TX5243) were also attenuated in C. elegans killing, although to a lesser extent than the fsr mutants. Complementation of fsrB (TX5266) with a 6-kb fragment containing the entire fsr locus restored virulence in both the nematode and the mouse peritonitis models. The fsr mutants were not impaired in their ability to colonize the nematode intestine. These data show that extracellular proteases and the quorum-sensing fsr system are important for E. faecalis virulence in two highly divergent hosts: nematodes and mice.
Applications of DMD-based Inhomogeneous Illumination Photoluminescence Imaging for Silicon Wafers and Solar Cells (2017)

Under inhomogeneous illumination, a silicon wafer or solar cell can generate lateral current flow due to gradients in the quasi Fermi energy levels. This lateral current flow is impacted by a few of the key electrical parameters of the sample, such as emitter sheet resistance, series resistance and diffusion length. This lateral current flow can be directly correlated to the spatially resolved luminescence intensity from the photoluminescence image. Therefore, photoluminescence imaging with inhomogeneous illumination can provide additional information, which is usually difficult to extract from a conventional photoluminescence image at open circuit voltage.
In Vivo Activation of Dendritic Cells and T Cells duringSalmonella entericaSerovar Typhimurium Infection (2001)

The present study was initiated to gain insight into the interaction between splenic dendritic cells (DC) and Salmonella enterica serovar Typhimurium in vivo. Splenic phagocytic cell populations associated with green fluorescent protein (GFP)-expressing bacteria and the bacterium-specific T-cell response were evaluated in mice given S. enterica serovar Typhimurium expressing GFP and ovalbumin. Flow cytometry analysis revealed that GFP-positive splenic DC (CD11c+ major histocompatibility complex class II-positive [MHC-II+] cells) were present following bacterial administration, and confocal microscopy showed that GFP-expressing bacteria were contained within CD11c+ MHC-II+ splenocytes. Furthermore, splenic DC and T cells were activated following Salmonella infection. This was shown by increased surface expression of CD86 and CD40 on CD11c+ MHC-II+ cells and increased CD44 and CD69 expression on CD4+ and CD8+ T cells. Salmonella-specific gamma interferon (IFN-gamma)-producing cells in both of these T-cell subsets, as well as cytolytic effector cells, were also generated in mice given live bacteria. The frequency of Salmonella-specific CD4+ T cells producing IFN-gamma was greater than that of specific CD8+ T cells producing IFN-gamma in the same infected animal. This supports the argument that the predominant source of IFN-gamma production by cells of the specific immune response is CD4+ T cells. Finally, DC that phagocytosed live or heat-killed Salmonella in vitro primed bacterium-specific IFN-gamma-producing CD4+ and CD8+ T cells as well as cytolytic effector cells following administration into naïve mice. Together these data suggest that DC are involved in priming naïve T cells to Salmonella in vivo.