gebhart/patrepeval huggingface dataset:
Abstract 4445: Novel antibody-dendrimer conjugates efficiently complex plasmid DNA (2011)

Abstract Introduction: Cationic polymers, such as polylysine, polyethylenimine, or polyamidoamine (PAMAM)-based dendrimers, form complexes with nucleic acids. However, their potential applications in nanomedicne as non-viral vectors for delivering therapeutic genes or siRNAs remain a challenge. One approach to improve selectivity and potency of a dendrimeric nanoparticle may be achieved by conjugation with an antibody that internalizes upon binding to target cells. In this study, we describe the synthesis and initial characterization of a novel immunoconjugate, designated E1-G5/2, which was made by the modular Dock-and-Lock (DNL) method to comprise half of a generation 5 (G5) PAMAM dendrimer (G5/2) site-specifically linked to a stabilized dimer of Fab derived from hRS7, a humanized antibody that is rapidly internalized upon binding to the Trop-2 antigen expressed on various solid cancers. Methods: E1-G5/2 was prepared by combining two self-assembling modules, AD2-G5/2 and hRS7-Fab-DDD2, under mild redox conditions, followed by purification on a Protein L column. To make AD2-G5/2, we derivatized the AD2 peptide with a maleimide group to react with the single thiol generated from reducing a G5 PAMAM with a cystamine core and used reversed-phase HPLC to isolate AD2-G5/2. We produced hRS7-Fab-DDD2 as a fusion protein in myeloma cells. The molecular size, purity and composition of E1-G5/2 were analyzed by size-exclusion HPLC, SDS-PAGE, and Western blotting. The biological functions of E1-G5/2 were assessed by binding to an anti-idiotype antibody against hRS7, a gel retardation assay, and a DNase protection assay. Results: E1-G5/2 was shown by size-exclusion HPLC to consist of a major peak (&amp;gt;90%) flanked by several minor peaks. The three constituents of E1-G5/2 (Fd-DDD2, the light chain, and AD2-G5/2) were detected by reducing SDS-PAGE and confirmed by Western blotting. Anti-idiotype binding analysis revealed E1-G5/2 contains a population of antibody-dendrimer conjugates of different size, all of which are capable of recognizing the anti-idiotype antibody, thus suggesting structural imperfections in the commercial supply of the G5 dendrimer. Gel retardation assay showed E1-G5/2 was able to maximally condense plasmid DNA at a charge ratio of 6:1 (+/-), with the resulting dendriplexes completely protecting the complexed DNA from degradation by DNase I. Conclusion: The DNL method can be used to build dendrimer-based nanoparticles that are targetable with antibodies. Such agents may have improved properties as carriers of drugs, plasmids, or siRNAs for diverse applications in vitro and in vivo. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4445. doi:10.1158/1538-7445.AM2011-4445
Draft Genome Sequence of “ Candidatus Methanomethylophilus” sp. 1R26, Enriched from Bovine Rumen, a Methanogenic Archaeon Belonging to the Methanomassiliicoccales Order (2016)

Here, we present the draft genome of "Candidatus Methanomethylophilus" sp. 1R26, a member of the newly described Methanomassiliicoccales order of Euryarcheaota. The enrichment culture was established from bovine rumen contents and produced methane from trimethylamine and methanol. The draft genome contains genes for methanogenesis from methylated compounds.
Enhancing wireless location privacy using silent period (2005)

The advance of ISM-band radio-based tracking systems (for example, wireless LAN-based tracking system) extends the application of location-based services (LBS), but it also threatens to allow the movement of users to be tracked when they are transmitting frames. Several protection methods based on periodic address updates have already been proposed. However, new correlation attacks, which utilize the correlation between the old and new addresses of the same node, can defeat current protection methods. To combat such attacks, we propose the concept of a silent period. A silent period is defined as a transition period between the use of new and old pseudonyms, when a node is not allowed to disclose either the old or the new address. Through analysis, we find that a silent period should contain a constant period and a variable period. The effect of the constant period is to mix the spatial relation between the node's disappearing points and emerging points. The variable period mixes the temporal relation between the node's disappearing times and emerging times. We evaluate the performance of the silent period through simulation. The results show that the silent period proposal significantly reduces the duration of time a node can be tracked continuously. There are still many open research problems before random address can be implemented to protect wireless location privacy, but silent period protocol is the first step to realizing it.
ACTIVE AERODYNAMIC BLADE CONTROL DESIGN FOR LOAD REDUCTION ON LARGE WIND TURBINES (2009)

Through numerical simulations that use trailing edge flaps as active aerodynamic load control devices on wind turbines that range from 0.6MW-5MW rated power, a 20-32 % reduction in blade root flap bending moments was achieved. This allows the turbine blade lengths to be increased, without exceeding origi-nal fatigue damage on the system, resulting in larger swept ro-tor area. This study developed and simulated several indepen-dent flap control designs (including tip deflection and tip rate de-flection feedback) that seamlessly integrated with existing pitch control strategies to reduce loads sufficiently to allow 10 % ro-tor extension and increased energy capture (see reference [1] for methodology). 1
Where on the Body is the Best Place for a Personal Imaging System? (2001)

This chapter considers the fundamental question of where on the body the imaging system should be situated. In terms of image acquisition and display, various systems have been tried. Among these is the author's wearable radar vision system for the visually challenged. This system is introduced, described, and compared with other systems.
The Spooner Collection of British Watercolours: At the Courtauld Institute Gallery (2006)

Catalogue d'une exposition présentée en 2005-2006 à la Courtauld Institute Gallery de Londres, au Wordsworth Trust de Grasmere et à la Huntington Library de San Marino (Calif.)
Multi-scaling models of TCP/IP and sub-frame VBR video traffic (2001)

Recent measurement and simulation studies have revealed that wide area network traffic displays complex statistical characteristics-possibly multifractal scaling-on fine timescales, in addition to the well-known property of self-similar scaling on coarser timescales. In this paper we investigate the performance and network engineering significance of these fine timescale features using measured TCP and MPEG2 video traces, queueing simulations and analytical arguments. We demonstrate that the fine timescale features can affect performance substantially at low and intermediate utilizations, while the longer timescale self-similarity is important at intermediate and high utilizations. We relate the fine timescale structure in the measured TCP traces to flow controls, and show that UDP traffic — which is not flow controlled-lacks such fine timescale structure. Likewise we relate the fine timescale structure in video MPEG2 traces to sub-frame encoding. We show that it is possibly to construct a relatively parsimonious multi-fractal cascade model of fine timescale features that matches the queueing performance of both the TCP and video traces. We outline an analytical method to estimate performance for traffic that is self-similar on coarse timescales and multi-fractal on fine timescales, and show that the engineering problem of setting safe operating points for planning or admission controls can be significantly influenced by fine timescale fluctuations in network traffic. The work reported here can be used to model the relevant characteristics of wide area traffic across a full range of engineering timescales, and can be the basis of more accurate network performance analysis and engineering.
Cloning and nucleotide sequence of the oriT region of the IncI1 plasmid R64 (1988)

The nucleotide sequence at the oriT region of the IncI1 plasmid R64 was determined. A recombinant plasmid carrying a 141-base-pair R64 sequence was mobilized with a normal frequency, while a plasmid carrying only 44 base pairs of this R64 sequence was mobilized with a frequency 1/10 that of the original plasmid. The oriT region of the R64 plasmid contains two inverted-repeat sequences.
Processing of ASTM Grade 12 Titanium Alloy and Its Mechanical and Corrosion-Resistant Properties (1986)

A 5 t ingot of ASTM G12 titanium alloy (Ti-0.8Ni-0.3Mo) was melted, and the plates, sheets, coils, bars and thin-all seam-welded tubes were manufactured. The processability of G12 alloy has proved almost similar to that of commercially pure titanium grades. It has been clarified that G12 alloy had several advantages in the mechanical property compared with titanium alloys G7 and G3; (1) higher strength at elevated temperatures which leads to higher maximum allowable stress in materials design, (2) larger Young's Modulus and (3) higher fatigue strength. The crevice corrosion tests for G12 alloy as well as G7 and G2 alloys have revealed that G12 alloy was quite superior to G2 and was close to G7 in the resistance to crevice corrosion in hot chloride solutions. It can be expected that the industrial application of G12 alloy will increase owing to its attractive characteristics.
A novel translocation, t(9;11)(q33;q23) involving the HRX gene in an acute monocytic leukemia. (1993)

A t(9;11)(q33;q23) has been detected by chromosome painting with chromosome 11- and chromosome 9-specific probes in blast cells of a child with acute monocytic leukemia. Using a YAC clone spanning the usual breakpoint region of translocations of acute leukemias, it was shown that the breakpoint was effectively within the same region of the band 11q23. This was confirmed by Southern blot studies that showed the localization of the translocation breakpoint between the 6th and 8th exons of the HRX gene. The implication of the HRX gene in t(9;11)(q33;q23) is a novel example of the diversity of translocations involving this gene in hemopoietic disorders. Sequencing DNA in the vicinity of the breakpoints should help to understand the reason of the localization of the recombination hot spot at band 11q23.
<title>Limiting role of the mass transfer of gases in laser thermochemical reactions on the surface of solids</title> (1990)

Growth kinetics of porous layers produced by gas-transfer controlled thermochemical reactions is described. The analysis of heat and mass-transfer problem allows to find the optimal regimes of laser treatment of solids. 1. GROWTH KINETICS OF SURFACE LAYERS The limiting role of gas transfer is characteristic of various processes in volved in the growth of solid layers of a new phase on the exposed surface'' in the course of laser oxidation and reduction of metals and laser synthesis and decomposition of nitrides and other compounds high-temperature superconductors included. When analyzing laser thermochemical reactions taking place in solids and involving the uptake or release of gases the absorbed energy flux q will be considered constant the thermooptical effects2 being disregarded. Let laser radiation incident on the surface of the original substance A give rise to a layer of a new phase B with a thickness of s through which a gas C migrates. We write the pertinent chemical decomposition and synthesis reactions in the form A 4 B + C and A + C ) B respectively. The direction of the reaction is determined by the sign of the Gibbs thermodynamic potential tG /H + TM where tH and tS are the changes of the enthalpy and entropy of the system respectively. According to the chemical equilibrium theory the pressure at the chemical reaction front is related to temperature by the Arrenius law:
Artificial Aging of Mobile Devices Using a Simulated GSM/GPRS Network (2013)

The analysis of mobile devices is a fast moving area in digital forensics. Investigators frequently are challenged by devices which are not supported by existing mobile forensic tools. Low level techniques like de-soldering the flash memory chip and extracting its data provide an investigator with the exhibits internal memory, however, the interpretation of the data can be difficult as mobile device and flash chip manufacturers use their own proprietary techniques to encode and store data. The approach presented in this paper helps investigators to analyze this proprietary encoding by feeding a reference device identical to the exhibit with real data in a controlled way. This "artificial ageing" of the reference device is achieved using an isolated GSM/GPRS network plus additional software in a lab environment. After the ageing process is completed, the internal memory of the reference device can be acquired and used to reverse engineer the high level file system and the encoding of the data previously fed to the phone, like received SMS messages or calls. When sufficient knowledge about the interpretation of the memory image has been built up, it can be applied to the original evidence in order to analyze data and files relevant for the case. The successful operation of the solution is demonstrated in a proof of concept for SMS messages.
Superposition Principle and Reciprocity for Pressure Transient Analysis of Data From Interfering Wells (2009)

Summary Based on a linearized model for the isothermal flow of a single, compressible phase through a reservoir of arbitrary shape with impermeable or constant-pressure boundaries and spatially varying, anisotropic rock properties, we develop a multiwell extension of the superposition principle and re-examine the question of reciprocity between wells that may be modeled as point sinks or as extended sinks. In the latter case, we find that the answer depends on the well-bore boundary conditions: Reciprocity holds for infinite conductivity wells but fails to hold for spatially uniform sink strength. We also derive a multiwell generalization of the fractional transformation in the Laplace domain, which adds skin and wellbore storage to a reservoir model, and find that its impact on reciprocity is neutral: It preserves reciprocity if it holds for the reservoir model.
Synthetic Nucleosides and Nucleotides. XXXV. Synthesis and Biological Evaluations of 5-Fluoropyrimidine Nucleosides and Nucleotides of 3-Deoxy-.BETA.-D-ribofuranose and Related Compounds. (1995)

1-O-Acetyl-2,5-di-O-p-chlorobenzoyl-3-deoxy-D-ribofuranose (1), derived from the antibiotic cordycepin was coupled with trimethylsilylated derivatives (2a-c) of N4-propionylcytosine, N4-p-toluoyl-5-fluorocytosine and 5-fluorouracil in the presence of trimethylsilyl trifluoromethanesulfonate (TMS-triflate) to give fully acylated nucleosides (3a-b and 3d, respectively). Selective removal of the N4-propionyl group of 3a by treatment with hydrazine hydrate gave 2',5'-di-O-p-chlorobenzoyl-3'-deoxycytidine (4). Deamination of 4 with sodium nitrite in trifluoroacetic acid afforded 2',5'-di-O-p-chlorobenzoyluridine (3c) in good yield. Compounds 3a-d were saponified to give free 3'-deoxycytidine (5a), 5-fluoro-3'-deoxycytidine (5b), 3'-deoxyuridine (5c), and 5-fluoro-3'-deoxyuridine (5d), respectively. These 3'-deoxyribonucleosides (5a-d) were then converted to corresponding 5'-monophosphate and further phosphorylated to the 5'-triphosphates by the phosphoroimidazolidate method. The nucleosides (5a-d) were examined for growth-inhibitory effects on mouse leukemic L5178Y cells, and their IC50 values (microgram/ml) were 1.8, 33, 6.5, and 18, respectively. On the other hand, the antiviral activities of these compounds on a rhabdovirus, infectious hematopoietic necrosis virus (IHNV), were moderate (IC50 = 100-500 micrograms/ml in CHSE-214 cells). The 5'-triphosphates showed remarkable inhibitory effects on DNA polymerase beta and DNA polymerase alpha-primase purified from testes of the cherry salmon, Oncorhynchus masou, but not on common DNA polymerase alpha from same source.
Age, Weight and the Genetics of Sexual Maturation in the Platyfish, Xiphophorus maculatus (1983)

William D. McKenzie, Jr., David Crews, Klaus D. Kallman, David Policansky, Joel J. Sohn, Age, Weight and the Genetics of Sexual Maturation in the Platyfish, Xiphophorus maculatus, Copeia, Vol. 1983, No. 3 (Aug. 16, 1983), pp. 770-774
Synthesis of Real-Time Observers from Past-Time Linear Temporal Logic and Timed Specification (2019)

Fault-tolerant architectures are mandatory to ensure the robustness of autonomous robots performing missions in complex and uncertain environments. The first step of a fault-tolerant mechanism is the detection of a faulty behavior of the system. It is then important to provide tools to help robot developers specify relevant observers. It is moreover crucial to guarantee a correct implementation of the observers, i.e. that the observers do not miss data and do not trigger unsuitable recovery actions in case of false detection. In this paper, we propose a specification language for observers that uses Past-Time LTL to express complex formulas on data produced by software components, and timed constraints on the evaluations of these formulas. We moreover provide an implementation of this specification that guarantees a real-time evaluation of the observers. We briefly describe the observers we have specified for a patrolling mission, and we evaluate the performance of our approach compared to state of the art on a benchmark in which we detect errors on a laser range sensor.
Recent advances in digital latching ferrite devices (2005)

This paper is concerned with the design and development of several new types of non-reciprocal latching ferrite devices. These devices have been designed to exhibit improved electrical performance at reduced per unit cost. Some of the described devices include (1)a single toroiddigital waveguide phase shifter, (2) a second generation temperature compensated, high average power phase shifter, (3) a digital strip transmission line phase shifter, (4) a miniaturized slow wave phase shifter, and (5) a latching ferrite waveguide cutoff switch. In addition to describing the design and operating characteristics of these devices, the results of recent experiments with digital reciprocal phase shifters are presented.
The Deficit Is Not a Well-Defined Measure of Fiscal Policy (1988)

Notwithstanding its widespread use, the government's deficit is not a well-defined measure of fiscal policy from the perspective of neoclassical economics; the equations of neoclassical models do not define the deficit. Rather than being a fundamental economic concept, the deficit is an arbitrary cash flow accounting construct with no necessary relation to the true stance of fiscal policy. Although the deficit is supposed to indicate how the burden of paying for the government's consumption is spread across different generations, actual changes in the measured deficit in the United States have had little if any relation to changes in the burden imposed by the government on different generations. The deficit's lack of definition is illustrated with a simple model, and the potential for misreading fiscal policy is discussed with U.S. fiscal policy in the 1980s as an example. In this article, creation of present value generational accounts are called for that would properly measure the intergenerational stance of fiscal policy.
Spatiotemporal Analysis of Gel Eating Behavior Based on Multipoint Tongue Pressure Sensing (2016)

This paper proposes a spatiotempolal analysis method of the tongue behavior in eating the nursing-care gel. First, we measure the tongue pressure in the process of eating the gel, by attaching the multipoint pressure sensor at the palate. Then, we introduce Lissajous figure drawn with two pressure waveforms measured at two different points. From the figure, we define three feature values: the pressure level, the pressure distribution angle, and the pressure phase difference. Finally, we confirm the correlations between the feature values and the values of sensory evaluation about the texture. The proposed method has a potential to be a useful tool for investigating the relationship between the tongue behavior and the texture of gel.
The ionization of organic molecules by slow positrons (1995)

The ionization of organic molecules by positrons having energies above and below their positronium formation thresholds is reviewed. The sensitivity of sub-positronium ionization yields to chemical and structural properties of the molecules is discussed, and possible mechanisms for ionization and fragmentation are suggested. Plans are presented for future experiments to further elucidate mechanisms and to search for evidence of positronium compound formation.
P-177 Long-term Safety of Adalimumab in Pediatric Patients with Crohnʼs Disease (2013)

rare and include worsening of demyelinating diseases, and neuropathies, often commencing months following treatment.to date. METHODS: We report the case of a 7 year old boy who was diagnosed with ulcerative colitis and primary sclerosing cholangitis at 6 and half years of age, who continued to have mild disease, manifested primarily by ongoing hematochezia and anemia despite Balsalazide and 6-Mercaptopurine therapy. His course to this point was unremarkable and his disease severity was mild with a Pediatric Ulcerative Colitis Score of 30. He was admitted for blood transfusion and underwent endoscopy and colonoscopy. He then received his first infusion of Infliximab. Other than mild sinus arrhythmia, he tolerated this well. There were no other notable events surrounding the infusion, he was interacting appropriately with normal vital signs and was discharged home. RESULTS: Within 6 hours of his infusion, he awoke from sleep from a severe headache and developed acute nausea and emesis. Half an hour later, he was slumped to one side and parents brought to a local emergency room. Upon arrival, he was completely unresponsive and emergently intubated. Given slight hypotension, he was suspected to have an anaphylactic reaction and received intravenous fluids, packed red blood cells and Epinephrine. Once transferred to our pediatric center, CT image of his brain revealed multifocal bilateral hypodense lesions with surrounding edema involving multiple lobes, the pons, and brainstem. Left sided lesions were largest and consistent with parenchymal hemorrhage. He was started on hypertonic saline and mannitol and external ventricular drains were promptly inserted, but his initial intracranial pressures were low. MRI imaging confirmed bilateral multi-territory infarcts that appeared to be arterial in nature without evidence of arterial clots, or venous infarcts. A workup for infectious encephalitis was negative, as was investigation for underlying anatomic anomalies of his vasculature. His presentation was not in keeping with an underlying vasculitis. An echocardiogram showed a dilated left ventricle, but did not show evidence of an arterial-septal defect, intracardiac thrombi or other abnormality. CONCLUSIONS: Our patient met criteria for brain death within 5 days following his single dose of Infliximab. Family refused an autopsy. We report a case of acute hemorrhagic stroke and ultimately fatal outcome following an initial infusion of Infliximab. Although the etiology of this event remains unclear, the close temporal relation to the Infliximab infusion cannot be ignored. To our knowledge, such a life threatening reaction within hours of Infliximab infusion has not been reported.