gebhart/patrepeval huggingface dataset:
Effect of different agents onto multidrug resistant tumor cells revealed by fluorescence correlation spectroscopy (2008)

Fluorescence correlation spectroscopy (FCS) has been used to analyze the plasma membrane fluidity and heterogeneity of multidrug resistant cells. At the single cell level, the effects of different membrane agents present in the extra-cellular medium have been explored. Firstly, we reveal a modification of plasma membrane heterogeneities according to the addition of a fluidity modulator, benzyl alcohol. On the other hand, revertants such as verapamil and cyclosporin-A appear to act more specifically on the slow diffusion sites, such as lipids microdomains.
[Protein analysis of enzyme tablets]. (2001)

Enzyme tablets with butyrylcholine esterase (CHE) and peroxidase (POD) partly lose enzymatic activity during compaction at a pressure of 495 MPa. Compared to solutions of the original enzyme, no changes of ultraviolet absorbance and fluorescence intensity in the tablet solutions were found. Only small changes were observed in the far ultraviolet circular dichroism spectra. Neither missing nor additional bands were detected with polyacrylamide gel electrophoresis. Heated (150 degrees C) solid starting material with CHE and POD showed still part of its original enzymatic activity. The ultraviolet absorbance increased with continued heating until precipitation occurred. The circular dichroism spectra are changed clearly.
Mechanism of methylphosphonic acid photo-degradation based on phosphate oxygen isotopes and density functional theory (2019)

Methylphosphonic acid (MPn) is an intermediate in the synthesis of the phosphorus-containing nerve agents, such as sarin and VX, and a biosynthesis product of marine microbes with ramifications to global climate change and eutrophication. Here, we applied the multi-labeled water isotope probing (MLWIP) approach to investigate the C-P bond cleavage mechanism of MPn under UV irradiation and density functional theory (DFT) to simulate the photo-oxidation reaction process involving reactive oxygen species (ROS). The results contrasted with those of the addition of the ROS-quenching compounds, 2-propanol and NaN<sub>3</sub>. The degradation kinetics results indicated that the extent of MPn degradation was more under alkaline conditions and that the degradation process was more rapid at the initial stage of the reaction. The phosphate oxygen isotope data confirmed that one exogenous oxygen atom was incorporated into the product orthophosphate (PO<sub>4</sub>) following the C-P bond cleavage, and the oxygen isotopic composition of this free PO<sub>4</sub> was found to vary with pH. The combined results of the ROS-quenching experiments and DFT indicate that the C-P bond was cleaved by OH<sup>-</sup>/˙OH and not by other reactive oxygen species. Based on these results, we have established a mechanistic model for the photolysis of MPn, which provides new insights into the fate of MPn and other phosphonate/organophosphate compounds in the environment.
The management of fungating wounds (1999)

A critical overview of approaches to the management of fungating lesions.
Ingenious Genes: The Diverse Roles of Long Noncoding RNA in Regulatory Processes (2015)

Pioneers in the field of epigenetics provide thought-provoking discussions on classic aspects of epigenetics and on the newer, emerging areas. Up-to-date resource essential for those working in epigenetics and recommended reading for anyone new to epigenetics.
Miscibility in poly‐p‐phenylene terephthalamide/nylon 6 and nylon 66 molecular composites (1989)

Abstract Miscibility and crystallization of poly‐ p ‐phenylene terephthalamide (PPTA)/nylon 6 and nylon 66 composites prepared by coagulation of isotropic ternary sulfuric acid solutions were studied. The apparent crystallinity of nylon 6 and nylon 66 in molecular composites was investigated by differential scanning calorimetry and Fourier transform infrared spectroscopy. The solvation of nylon 6 crystals in PPTA matrix was observed when the PPTA content exceeds 70 wt%. Cross‐hydrogen bonding seems to be responsible for the virtual disappearance of nylon 6 crystals. Specific interaction between PPTA and nylon 6 macromolecules and phase separation during thermal treatment has been discussed.
Chemoresponsive assemblies of microparticles at liquid crystalline interfaces (2010)

Assemblies formed by solid particles at interfaces have been widely studied because they serve as models of molecular phenomena, including molecular self-assembly. Solid particles adsorbed at interfaces also provide a means of stabilizing liquid–liquid emulsions and synthesizing materials with tunable mechanical, optical, or electronic properties. Whereas many past studies have investigated colloids at interfaces of isotropic liquids, recently, new types of intercolloidal interactions have been unmasked at interfaces of liquid crystals (LCs): The long-range ordering of the LCs, as well as defects within the LCs, mediates intercolloidal interactions with symmetries that differ from those observed with isotropic liquids. Herein, we report the decoration of interfaces formed between aqueous phases and nematic LCs with prescribed densities of solid, micrometer-sized particles. The microparticles assemble into chains with controlled interparticle spacing, consistent with the dipolar symmetry of the defects observed to form about each microparticle. Addition of a molecular surfactant to the aqueous phase results in a continuous ordering transition in the LC, which triggers reorganization of the microparticles, first by increasing the spacing between microparticles within chains and ultimately by forming two-dimensional arrays with local hexagonal symmetry. The ordering transition of the microparticles is reversible and is driven by surfactant-induced changes in the symmetry of the topological defects induced by the microparticles. These results demonstrate that the orderings of solid microparticles and molecular adsorbates are strongly coupled at the interfaces of LCs and that LCs offer the basis of methods for reversible, chemosensitive control of the interfacial organization of solid microparticles.
Finite-State Non-Concatenative Morphotactics (2000)

Finite-state morphology in the general tradition of the Two-Level and Xerox implementations has proved very successful in the production of robust morphological analyzer-generators, including many large-scale commercial systems. However, it has long been recognized that these implementations have serious limitations in handling non-concatenative phenomena. We describe a new technique for constructing finite-state transducers that involves reapplying the regular-expression compiler to its own output. Implemented in an algorithm called compile-replace, this technique has proved useful for handling non-concatenative phenomena; and we demonstrate it on Malay full-stem reduplication and Arabic stem interdigitation.
Implementation of the SEMI 'Generic Equipment Model' using object-based cell technology (2002)

A method was sought for developing a general-purpose automation platform for a large-capacity 4 Mbit DRAM production facility. Various technological approaches were evaluated ranging from simple messaging systems to programmer toolkits to an 'object-based cell' approach that meets the stated goal of 'enabling' the process engineer. Because of the message passing and dynamic binding nature of object-based cell technology, principal elements of the SEMI (Semiconductor Equipment and Materials International) Generic Equipment Model (GEM) could be implemented in a straightforward manner, including event classes, finite state machine control, spooling, logging, material movement control, data reporting, and process program management. The SEMI GEM definition document was used as a generic template for specifying particular production applications as well as the general capabilities for equipment management.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Mechanisms for Noncontact Anterior Cruciate Ligament Injuries (2010)

Based on when the sudden changes in joint angular motion and the peak vertical ground-reaction force occurred, it is likely that the anterior cruciate ligament injury occurred approximately 40 milliseconds after IC. The kinematic patterns were surprisingly consistent among the 10 cases. All players had immediate valgus motion within 40 milliseconds after IC. Moreover, the tibia rotated internally during the first 40 milliseconds and then external rotation was observed, possibly after the anterior cruciate ligament had torn. These results suggest that valgus loading is a contributing factor in the anterior cruciate ligament injury mechanism and that internal tibial rotation is coupled with valgus motion. Prevention programs should focus on acquiring a good cutting and landing technique with knee flexion and without valgus loading of the knee.
A front-end circuit with active spike and LFP separation via a switched capacitor filter structure for neural recording applications (2012)

A fully differential front-end circuit for a neural recording system with spike and Local Field Potential (LFP) separation by means of switched capacitor (SC) filters is reported in this paper. In many neural recording chips the transmitter is the most power hungry element and defines the number of channels on a chip. With a separation of the neural signal, it is possible to reduce the data rate and so the power consumption of the transmitter without any reduction of resolution in the quantized signal. The system consists of a low-noise-amplifier (LNA), an anti-aliasing-filter with tunable gain and a corner frequency at 50kHz to enable SC circuits with a clock frequency of 100kHz. Two SC biquad filters with tunable gain and tunable corner frequency are implemented to split the spikes and the LFP from each other and to amplify the sub signals to the full swing. Both channels provide an input referred noise of 3.8μVrms in the passband and the whole front-end circuit has a power dissipation of 52μW. A prototype is fabricated in a standard 0.18μm CMOS process and tested successfully.
In cryptozoospermia or severe oligozoospermia is sperm freezing useful? (2014)

International audience
Marketing the Goverment (2000)

With the turbulence experienced in management and information technology during the later part of the 2"âi millenium, and the dawn of the vet to be clear 3rd millennium, there is no more conviction needed nor, compromise to be reached between the stale and the citizens in terms of service delivery. This requires recognition of the citizens as customers, and the gateway left for the government being that of embracing the 'market failure' principle in order to come out of this service delivery trap and chaos.\nThis article is therefore out to invite and persuade the reader to investigate by him/herself the issues discussed here that necessitates invoking the marketing concept, and specifically so the adaptation of the relationship marketing paradigm in the delivery of services to the public. So. this article, besides defining the conceptâmarketing the government\nand exploring areas that necessitates adoption of the marketing concept into the public sector does not detail how to go about the tedious marketing process and procedure. Thus, the scope is limited to exploring the areas related to economics, organizations, and human behavior to assist in explaining how far market concepts can resolve the chaos and prevailing service delivery trap to the public. The reader is also persuaded to discover the point ofconvergence between marketing, the human and organizational needs, and behavior. Once this has been achieved, the reader will be expected to perceive the relational exchange process of marketing the government. Discussed in this article as well, is the interrelationship between marketing the government and investment, reinvention
XPS and EXAFS characterization of Ziegler–Natta catalyst systems (2008)

Abstract A series of Ziegler–Natta catalysts, with and without diisobutylphtalate as an internal donor, were modified with alkylaluminum (triethylaluminum or diethylaluminum chloride) and an external donor (diisopropyldimethoxysilane or methyltrimethoxysilane), in increasing complexity. The resulting catalysts were characterized by X‐ray photoelectron spectroscopy and extended X‐ray absorption fine structure analysis (EXAFS) and evaluated in propylene polymerization. According to EXAFS oscillation, systems bearing diisobutylphtalate as an internal donor displayed a well‐defined pattern, suggesting a more ordered structure around the Ti atom. EXAFS data treatment provided two TiCl distances: ∼ 1.96 and 2.5 Å. The former was shown to have a linear dependence on the Ti (2p 3/2 ) binding energy as the catalyst is modified with TEA or with external donors. Conversely, the longest distance is reduced as Ti (2p 3/2 ) binding energy increases with catalyst modification. Correlations involving the catalyst activity, Ti (2p 3/2 ) binding energy, and TiCl distances could be denoted. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
ChemInform Abstract: Molecularly‐Mediated Assemblies of Plasmonic Nanoparticles for Surface‐Enhanced Raman Spectroscopy Applications (2013)

Abstract Review: 217 refs.
An Improved PDR/Magnetometer/Floor Map Integration Algorithm for Ubiquitous Positioning Using the Adaptive Unscented Kalman Filter (2015)

In this paper, a scheme is presented for fusing a foot-mounted Inertial Measurement Unit (IMU) and a floor map to provide ubiquitous positioning in a number of settings, such as in a supermarket as a shopping guide, in a fire emergency service for navigation, or with a hospital patient to be tracked. First, several Zero-Velocity Detection (ZDET) algorithms are compared and discussed when used in the static detection of a pedestrian. By introducing information on the Zero Velocity of the pedestrian, fused with a magnetometer measurement, an improved Pedestrian Dead Reckoning (PDR) model is developed to constrain the accumulating errors associated with the PDR positioning. Second, a Correlation Matching Algorithm based on map projection (CMAP) is presented, and a zone division of a floor map is demonstrated for fusion of the PDR algorithm. Finally, in order to use the dynamic characteristics of a pedestrian’s trajectory, the Adaptive Unscented Kalman Filter (A-UKF) is applied to tightly integrate the IMU, magnetometers and floor map for ubiquitous positioning. The results of a field experiment performed on the fourth floor of the School of Environmental Science and Spatial Informatics (SESSI) building on the China University of Mining and Technology (CUMT) campus confirm that the proposed scheme can reliably achieve meter-level positioning.
Dissecting and Tuning Primer Editing by Proofreading Polymerases (2021)

ABSTRACT Proofreading polymerases have 3’ to 5’ exonuclease activity that allows the excision and correction of mis-incorporated bases during DNA replication. In a previous study, we demonstrated that in addition to correcting substitution errors and lowering the error rate of DNA amplification, proofreading polymerases can also edit PCR primers to match template sequences. Primer editing is a feature that can be advantageous in certain experimental contexts, such as amplicon-based microbiome profiling. Here we develop a set of synthetic DNA standards to report on primer editing activity and use these standards to dissect this phenomenon. The primer editing standards allow next-generation sequencing-based enzymological measurements, reveal the extent of editing, and allow the comparison of different polymerases and cycling conditions. We demonstrate that proofreading polymerases edit PCR primers in a concentration-dependent manner, and we examine whether primer editing exhibits any sequence specificity. In addition, we use these standards to show that primer editing is tunable through the incorporation of phosphorothioate linkages. Finally, we demonstrate the ability of primer editing to robustly rescue the drop-out of taxa with 16S rRNA gene-targeting primer mismatches using mock communities and human skin microbiome samples.
Quantitation of bacteria in cerebrospinal fluid and blood of children with meningitis and its diagnostic significance (1984)

Cerebrospinal fluid (CSF) specimens from pediatric patients with meningitis were examined for their concentration of microbes and the relationship of this count to the bacteremia levels, microscopy results, and polymorphonuclear leukocyte concentration. A total of 2,031 consecutive CSF specimens were analyzed, of which 63 (3.1%) were positive by culture from the same number of patients. We observed that 85% of the total CSF specimens positive for Haemophilus influenzae type b, Streptococcus agalactiae, Streptococcus pneumoniae, and Neisseria meningitidis had counts in excess of 10(3) CFU/ml, with 56% of the specimens exceeding 10(5) CFU/ml. A correlation existed between the number of organisms present in the CSF and blood. For example, from a total of 22 patients who had counts of H. influenzae greater than 10(3) CFU/ml in the CSF, 16 or 73% had levels of bacteremia greater than 10(3) CFU/ml. It was also noted that the bacterial concentration had a profound effect on the sensitivity of microscopy. The percentage of positive results increased from 25% with less than or equal to 10(3) CFU/ml to 60% in the range of greater than 10(3) to 10(5) CFU/ml and to 97% at concentrations of greater than 10(5) CFU/ml. Furthermore, a significant correlation (P less than 0.01) was noted between the concentration of bacteria in the CSF and the number of polymorphonuclear leukocytes observed on microscopy.
Sputtered Schottky barrier solar cells on p-type GaAs (1983)

Schottky barrier solar cells have been produced on single crystal p-type GaAs by sputtering gold to form the barrier. The rectifying action for the gold sputtered p-type device is unique to the method since normal thermal evaporated gold onto p-type produced Ohmic contacts. The opposite behavior was observed for n-type GaAs. Barrier heights have been measured for both p-type (sputtered) and n-type (thermal) diodes using current-voltage and capacitance-voltage methods and are 0.90 and 0.95 eV, respectively. The power conversion efficiencies without AR coatings have values of 6.56% (p) and 5.58% (n). Deep level transient spectroscopy has been used to identify the trap center concentrations and energy levels for both diodes to account for the relatively large dark currents in the p-type (sputtered) diodes.
Relative Permeability Hysteresis: Laboratory Measurements and a Conceptual Model (1995)

Summary Relative permeability hysteresis has been measured for a water-wet outcrop rock sample and a mixed-wet reservoir core. For the oil phase, imbibition and drainage relative permeability curves differed significantly. The difference was much less pronounced for the water phase. Scanning curves, which characterize transitions between imbibition and drainage curves, were also measured. A notable characteristic of the oil relative permeability scanning curves is their reversibility; along most of the length of a scanning curve, oil relative permeability exhibits no hysteresis. A proposed mechanism for the reversible behavior is pinning of water/oil interfaces on surfaces of rock grains. Pinned interfaces remain anchored at fixed positions on grains despite changes in interface curvature and contact angle. In water-wet samples, pinning can occur as a result of contact-angle hysteresis. In mixed-wet rock, pinning can occur at the boundaries between water- and oil-wet grain surfaces. As long as interfaces remain pinned, pore-level fluid geometry is a function of saturation only and does not depend on the direction of saturation change.
A new online secondary path modelling method for feedforward active noise control systems (2008)

An online secondary path modelling method using a white noise as a training signal is required in many applications of active noise control (ANC) to ensure convergence of the system. Not continually injection of white noise during system operation makes the system more desirable. The purposes of the proposed method are two folds: controlling white noise by preventing continually injection, and benefiting white noise with a larger variance. The modelling accuracy and the convergence rate increase when a white noise with larger variance is used, however larger the variance increases the residual noise, which decreases performance of the system. This paper proposes a new approach for online secondary path modelling in feedfoward ANC systems. The proposed algorithm uses the advantages of the white noise with larger variance to model the secondary path, but the injection is stopped at the optimum point to increase performance of the system. Comparative simulation results shown in this paper indicate effectiveness of the proposed approach in controlling active noise.
Factors influencing glove use in student nurses. (2015)

All student nurses must be able to identify clinical situations when gloves are not indicated, using appropriate risk assessment.
Endoplasmic reticulum associated degradation preserves hematopoietic stem cell quiescence and self-renewal by restricting mTOR activity (2019)

Summary Many tissue-specific stem cells require quiescence to sustain stem cell pool and maintain lifelong tissue integrity. It remains unclear whether protein quality control is required for stem cells in quiescence when RNA content, protein synthesis and metabolic activities are significantly reduced. Here, we report that endoplasmic reticulum associated degradation (ERAD) is required to preserve the function of quiescent hematopoietic stem cells (HSC). The Sel1L/Hrd1 ERAD genes are enriched in the quiescent and inactive HSCs, and conditional knockout of Sel1L in hematopoietic tissues drives HSCs to hyper-proliferation which leads to reduced self-renewal and HSC depletion. ERAD deficiency induces a non-apoptotic ER stress and activates unfolded protein response (UPR). Furthermore, Sel1L knockout leads to mTOR activation, and mTOR inhibition rescues the HSC defects in Sel1L knockout mice. Protein quality control is, therefore, tightly regulated and actively engaged in quiescent HSCs. Sel1L/Hrd1 ERAD maintains HSC quiescence and self-renewal via restricting mTOR activity.
Phosphatidylinositol promotes cholesterol transport and excretion (2003)

Administration of phosphatidylinositol (PI) to New Zealand White rabbits increases HDL negative charge and stimulates reverse cholesterol transport. Intravenously administered PI (10 mg/kg) associated almost exclusively with the HDL fraction in rabbits. PI promoted an increase in the hepatic uptake of plasma free cholesterol (FC) and a 21-fold increase in the biliary secretion of plasma-derived cholesterol. PI also increased cholesterol excretion into the feces by 2.5-fold. PI directly affects cellular cholesterol metabolism. In cholesterol-loaded macrophages, PI stimulated cholesterol mass efflux to lipid-poor reconstituted HDL. PI was about half as effective as cAMP at stimulating efflux, and the effects of cAMP and PI were additive. In cultured HepG2 cells, PI-enriched HDL also enhanced FC uptake from HDL by 3-fold and decreased cellular cholesterol synthesis and esterification. PI enrichment had no effect on the selective uptake of cholesterol esters or on the internalization of HDL particles. PI-dependent metabolic events were efficiently blocked by inhibitors of protein kinase C and the inositol signaling cascade. The data suggest that HDL-PI acts via cell surface ATP binding cassette transporters and signaling pathways to regulate both cellular and intravascular cholesterol homeostasis.
WIST: toolkit for rapid, customized LIMS development (2011)

WIST is implemented in Perl and may be obtained from http://vimss.sf.net under the BSD license.