gebhart/patrepeval huggingface dataset:
Exploring the Function-Location Nexus: Using Multiple Lines of Evidence in Defining the Subcellular Location of Plant Proteins (2009)

Defining the function of all proteins in an organism is one of the major objectives for biology in the coming decades. Here, we assess approaches used to determine subcellular protein location and discuss the relationship between protein location and function. It is important to recognize that targeting, accumulation, and the site of function are not necessarily interchangeable terms with respect to defining the location of a protein. Some proteins have tightly defined locations, whereas others have low specificity targeting and complex accumulation patterns. Location may be essential for function in some cases, but it may be much less important for other proteins. There is no single approach that can be considered entirely adequate for defining the in vivo location of all proteins. By combining approaches that assess targeting and accumulation of proteins, more confidence can be gained about localization. The strengths and weaknesses of different localization technologies are summarized, and some guidelines for performing combined targeting and accumulation assays are outlined.
Normal serum and lipoprotein-deficient serum give different expressions of excitability, corresponding to different stages of differentiation, in chicken cardiac cells in culture. (1982)

Monolayers of cardiac cells from 11-day-old chicken hearts have different properties when maintained in fetal calf serum or in a lipoprotein-deficient serum (LPDS). Cells in fetal calf serum have a resting potential near -60 mV; the rate of rise of the action potential is low (less than 10 V/sec); the action potential and the contraction are essentially unaffected by tetrodotoxin (TTX); and the beating properties are unaffected by muscarinic agents. Cells in LPDS have a resting potential near -75 mV, and a fast rise of the action potential (approximately equal to 100 V/sec) that is drastically decreased by TTX with a parallel abolition of contraction, and the beat is blocked by very low concentrations of muscarinic agonists. Cells that are physiologically fully responsive to TTX and to muscarinic agents have receptors that remain stable 24 hr after protein synthesis is blocked, whereas cells that are physiologically unresponsive to TTX and muscarinic agents have receptors that are rapidly degraded with half-lives between 9 hr (TTX receptor) and 14 hr (muscarinic receptor). Differences in the physiological and biochemical properties are accompanied by changes in the cholesterol contents of the cell membranes. The properties of cardiac cells cultured in normal serum are similar to those found for cells of chicken hearts in the very early embryonic stage, whereas those of cardiac cells cultured in LPDS correspond to the late embryonic stage.
Preparation of Fe-Oxipnictides Superconductors: Solid-State Sintering, High Pressure Synthesis and Flux Method (2008)

We have fabricated the F-doped LaFeAsO superconductors via three fabrication processes; (1) solid-state sintering, (2) high pressure (HP) synthesis and (3) crystallization from flux solution. Samples prepared by these processes have unique features depending on the process such as a large volume and uniformity of the sintered pellet, variety of the compositions in the high pressured pellet and potential of growing large single crystal using fluxes. In particular, the HP synthetic process has a unique advantage that the compositional variations in the compounds were realized by a large temperature gradient within a short reaction time.
Investigating barriers and facilitators to facility‐level births in rural Guatemala (2019)

ClinicalTrials.gov NCT02348840
Process Intensification – An Industrial Point of View (2007)

This chapter contains sections titled: Introduction Remarks on the Term Process Intensification Management Aspects Microreaction Technology Principal Features Catalytic Wall Reactors Simulation Introduction Molecular Simulations Quantum-chemical Calculations COSMO-RS Calculations Molecular-dynamics Calculations Monte Carlo Simulations in Project Valuation under Risk
PARP Inhibitors and Proteins Interacting with SLX4 (2023)

PARP inhibitors are small molecules currently used with success in the treatment of certain cancer patients. Their action was first shown to be specific to cells with DNA repair deficiencies, such as BRCA-mutant cancers. However, recent work has suggested clinical interest of these drugs beyond this group of patients. Preclinical data on relationships between the activity of PARP inhibitors and other proteins involved in DNA repair exist, and this review will only highlight findings on the SLX4 protein and its interacting protein partners. As suggested from these available data and depending on further validations, new treatment strategies could be developed in order to broaden the use for PARP inhibitors in cancer patients.
URINARY SOMATIC ANGIOTENSIN CONVERTING ENZYME AND SOLUBLE N-DOMAIN ISOFORMS (90 AND 65KDA) EXPRESSION AND CORRELATION WITH DIFFERENT NUTRITIONAL STATUS/CARDIOVASCULAR RISK PROFILE (2021)

Abstract Objective: Angiotensin converting enzyme (ACE) has been involved in physiopathology of several diseases including hypertension, diabetes, obesity and metabolic syndrome. The somatic ACE weights from 130–190 KDa and presents N- and C-domains. Two soluble N-domain isoforms have been described in human urine, weighting 65 and 90KDa. Studies have supported that N-domain ACE with 90KDa is a biomarker for hypertension, pre-eclampsia and inflammation. We evaluated the expression of somatic ACE and soluble N-domain isoforms (90 and 65KDa) in urine of children and adolescents with different nutritional status and cardiovascular risk profile. Design and method: The participants aged from 6 to 19 years were classified into four groups according to their BMI percentile; underweight (n = 51), normal weight (n = 53), overweight (n = 53) and obese (n = 49). Waist-height-ratio (WHtR) was used to assess cardiovascular risk profile dividing the participants into normal risk (n = 105) and high risk (n = 101). The urines were concentrated 10-fold and dialyzed with Tris-HCl pH 8 and pure water. Then, we performed western blot analysis using 50 mg of lyophilized urinary protein. ACE expression was proved against the polyclonal antibody Y1. Protein detection was performed by chemiluminescent and analysis was done in Image Labâ (BioRad) software utilizing total protein stain for normalization. Results: ACE expression is augmented in obese children when compared with normal weight children (0.09 vs 0.53 arbitrary units, p = 0,04). The higher cardiovascular risk group also presented increased expression of ACE (0.27 vs 0.09 arbitrary units, p = 0.046). The 90KDa N-domain isoform is frequently found in the high cardiovascular risk children (p = 0.02). Additionally, according to Spearman correlation test, the expression of 90KDa N-domain isoform correlates positively with waist circumference (cm), WHtR, BMI percentile and Z-score of BMI. Conclusions: Increased ACE expression in obese children contributes to higher cardiovascular risk once this enzyme biosynthesizes Angiotensin II which promotes blood pressure increase, sympathetic nervous system activation and release of glucocorticoids from adrenal gland. ACE expression is also augmented in children with high cardiovascular risk. Presence of 90 KDa N-domain ACE in urine of children and adolescents is a biomarker of poor prognostic for cardiovascular disease in childhood obesity.
Meta-regression analysis of the effect of lifestyle interventions on mediators of inflammation in postmenopausal breast cancer women with cardiovascular risk factors (2019)

Abstract Background: Chronic inflammation is associated with obesity, dyslipidemia, and different types of malignancies that may play a complex role in the risk of breast cancer (BC) recurrence and therapy related side effects. The purpose of this review was to determine the effects of lifestyle interventions on mediators of inflammation (C-reactive protein (CRP), interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α)) and other therapy related adverse events in postmenopausal BCS with cardiovascular related risk factors. Methods: A systematic search for relevant articles was performed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis criteria using available databases (PubMed, Scopus, The Cochrane Library, OpenGrey, Google Scholar and CENTRAL). Results: Twelve eligible randomized controlled trials (RCT) met the inclusion criteria. The mean ages of postmenopausal BC women ranged from 49 to 56 years. The meta-regression revealed that there was moderate evidence that MSE decreased the mean difference (MD) of inflammatory markers in BCS, with CVD risk factors (MD: 0.31; 95% CI: 0.05 to 0.57, P=0.02; heterogeneity: Tau² = 0. 12; Chi² = 106.30, df = 19, I² = 0. 50.4%). Conclusion: There is a moderate level of evidence on the effects of MSE on mediators of inflammation in postmenopausal BCS with cardiovascular risk factors. A number of shared risk factors between postmenopausal status and CVD should be considered, while treating postmenopausal BCS.
The Human Organism in a Changing Environment (1969)

The interaction between the forces of nature and the forces of human culture has continued with ever-increasing intensity up to the present day, and has now reached proportions that many people consider grave cause for alarm. Carried away by what he regarded as his 'conquest of nature', man has devoted a tremendous amount of intellectual effort towards devising more efficient ways of extracting greater benefits from the earth's resources; but he has given negligible thought in comparison to the ecological consequences of these activities. Several examples of the social, cultural and biological adaptations of man in response to the changes in the environment are provided suggesting that humans should be aware of the looming environmental crisis and its harmful effects on them in the future.
Imaging Seebeck drift of excitons and trions in MoSe 2 monolayers (2021)

Abstract Hyperspectral imaging at cryogenic temperatures is used to investigate exciton and trion propagation in MoSe 2 monolayers (ML) encapsulated with hexagonal boron nitride (hBN). Under a tightly focused, continuous-wave laser excitation, the spatial distribution of neutral excitons and charged trions strongly differ at high excitation densities. Remarkably, in this regime the trion distribution develops a halo shape, similar to that previously observed in WS 2 ML at room temperature and under pulsed excitation. In contrast, the exciton distribution only presents a moderate broadening attributed to a much less pronounced halo. Spatially and spectrally resolved luminescence spectra reveal the buildup of a significant temperature gradient at high excitation power, that is attributed to the energy relaxation of photoinduced hot carriers. We show, via a numerical resolution of the transport equations for excitons and trions, that the halo can be interpreted as thermal drift of trions due to a Seebeck term in the particle current. The model shows that the difference between trion and exciton profiles is simply understood in terms of the very different lifetimes of these two quasiparticles.
Weight and Size Reduction by Integrating Avionic Optical Components (2007)

Recent developments in the study of condensing several electro-optical properties such as single frequency light generation, modulation, light monitoring, multiplexing (Mux) and optical mode shaping into one monolithic, InP based photonic integrated circuit (PIC), together with advances in silicon planar light-guide circuits (PLC) allow the design of light-weight, compact, rugged, lower cost optical transmitters and receivers. Depending upon the avionics' transmission capacity application, one compact set of a coarse wave division multiplex (CWDM) transmitter and receiver, each with a single mode fiber (SMF) connection can provide full duplex transmission to 100 Gbps. These 2 components can replace 20 or more package bodies, 20 sets of lens, 20 submounts, 10 isolators, 20 fiber connections and 20 fibers with the corresponding reduction in weight and size. This paper summarizes the advantages of monolithic PIC and hybrid PLC optical components for transmission capacities up to 100 Gbps at individual wavelength channel speeds up to 10 Gbps using CWDM.
Autogenous Regulation of Histone mRNA Decay by Histone Proteins in a Cell-Free System (1987)

We tested the hypothesis that histone mRNA turnover is accelerated in the presence of free histone proteins. In an in vitro mRNA decay system, histone mRNA was degraded four- to sixfold faster in reaction mixtures containing core histones and a cytoplasmic S130 fraction than in reaction mixtures lacking these components. The decay rate did not change significantly when histones or S130 was added separately, suggesting either that the histones were modified and thereby activated by S130 or that additional factors besides histones were required. RecA, SSB (single-stranded binding), and histone proteins all formed complexes with histone mRNA, but only histones induced accelerated histone mRNA turnover. Therefore, the effect was not the result of random RNA-protein interactions. Moreover, histone proteins did not induce increased degradation of gamma globin mRNA, c-myc mRNA, or total poly(A)- or poly(A)+ polysomal mRNAs. This autoregulatory mechanism is consistent with the observed accumulation of cytoplasmic histone proteins in cells after DNA synthesis stops, and it can account, in part, for the rapid disappearance of histone mRNA at the end of S phase.
Novel azacrown ethers incorporating sugars: synthesis and complex formation (1989)

Azacrown ethers derived from amino-deoxy sugars in which the sugar moiety forms part of the macrocycle, or is pendant from it, have been synthesised and their complexing ability towards cations compared.
Space charge fields: a fringe technique for observing such fields during hologram writing in LiNbO_3 (1976)

An interference fringe method is reported that allows rapid monitoring of the large scale (nonsinusoidal) space charge field which develops during hologram writing with nonuniform light intensity in LiNbO(3). This large scale field is distinct from the sinusoidal space charge fields that constitute the hologram. The large scale field has been shown elsewhere to be important in that it affects the efficiency of hologram writing and leads to history effects. Using the fringe method, a dielectric breakdown process associated with the large scale field was observed which may have been responsible for some effects reported by Carlsen in his work on computer applications.
Introduction to the Special Section on the 2017 IEEE S3S Conference (2018)

The papers in this special section were presented at the 2017 IEEE S3S Conference, which was held Oct. 16 – 19, 2017 in Burlingame, CA.
Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations (2019)

Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.
Numerical simulation of flow past a circular base on PANS methods (2016)

A nonlinear partially averaged Navier-Stokes (PANS) method based on RNG k-e turbulence model is evaluated by a moderately high Reynolds number turbulence flow past a circular cylinder. The ratios of unresolved-to total kinetic energy (f k) and unresolved-to-total disspation (fe ) for PANS method is sensitive to the simulation result. Simulation results based on different fk are compared with the experimental data. The quantities including mean streamline velocity, mean normal velocity, streamlines and et al. are analyzed. The computational result is reasonable when fk is less than 0.6. The PANS method can be used in the simulation of high Reynolds number turbulence flow.
A Cystatin C Cleavage ELISA Assay as a Quality Control Tool for Determining Sub-Optimal Storage Conditions of Cerebrospinal Fluid Samples in Alzheimer’s Disease Research (2021)

Using this novel double indirect sandwich ELISA, absolute levels of the total and uncleaved cystatin C and the ratio of truncated cystatin C can be measured. This assay is an easily applicable tool which can be used to confirm that CSF biospecimen are fit-for-purpose for Alzheimer's disease research.
Introduction: Prediction of F-16XL Flight Flow Physics (2009)

This special section is the result of fruitful endeavors by an international group of researchers in industry, government laboratories and university-led efforts to improve the technology readiness level of their CFD solvers through comparisons with flight data collected on the F-16XL-1 aircraft at a variety of test conditions. These 1996 flight data were documented and detailed the flight-flow physics of this aircraft through surface tufts and pressures, boundary-layer rakes and skin-friction measurements. The flight project was called the Cranked Wing Aerodynamics Project (CAWAP), due to its leading-edge sweep crank (70 degrees inboard, 50 degrees outboard), and served as a basis for the International comparisons to be made, called CAWAPI. This highly focused effort was one of two vortical flow studies facilitated by the NATO Research and Technology Organization through its Applied Vehicle Panel with a title of Understanding and Modeling Vortical Flows to Improve the Technology Readiness Level for Military Aircraft. It was given a task group number of AVT-113 and had an official start date of Spring 2003. The companion part of this task group dealt with fundamentals of vortical flow from both an experimental and numerical perspective on an analytically describable 65 degree delta-wing model for which much surface pressure data had already been measured at NASA Langley Research Center at a variety of Mach and Reynolds numbers and is called the Vortex Flow Experiment - 2 (VFE-2). These two parts or facets helped one another in understanding the predictions and data that had been or were being collected.
Environmental effects on the energetics and photo-induced dynamics of molecular states (1994)

Environmental effects on electronic states of small mols. in solid rare gases and in liqs. have severe consequences on the photochem.; examples for valence, Rydberg and ionic states as well as the connection to photochem. dry etching are treated. The repulsive interaction of Rydberg states and the solvation of valence and ionic states are related to spectroscopic properties like gas-to-matrix shifts, Stokes shifts and linewidths. Differential shifts of Rydberg and valence states are illustrated for NO in rare gas matrixes and Fano-type line shapes are attributed to a coherent interaction of broad Rydberg multiphonon continua with valence zero phonon lines. For F2 in Ne a deperturbation of ion-pair and Rydberg states is demonstrated again to originate from a differential shift. For Cl2 the formation of addnl. states due to intermol. charge transfer from the matrix to the guest mols. is obsd. H2O serves as an example to show that repulsive surfaces are strongly modified if the character of the state changes from Rydberg type to valence type with internuclear distance. Information on the dynamics in the femtosecond time scale is derived from intensities in overtone progressions of resonance Raman spectra and it is demonstrated that the solvent induces predissocn. of the B3P0 +u of I2 state within 300 fs in CCl4, and within 40 fs in liq. Xe. 62 Refs. [on SciFinder (R)]
Fetal insulin and placental 3-O-methyl glucose clearance in near-term sheep. (1985)

It is difficult, if not impossible, to measure the placental transfer of glucose directly because of placental glucose consumption and the low A-V glucose difference across the sheep placenta. We have approached the problem of quantifying placental hexose transfer by using a nonmetabolized glucose analogue (3-O-methyl glucose) which shares the glucose transport system. We have measured the clearance by using a multisample technique permitting least squares linear computing to avoid the errors implicit in the Fick principle. The placental clearance of 3-O-methyl glucose was measured in the control condition and after the administration of insulin to the fetal circulation. A glucose clamp technique was used to maintain constant transplacental glucose concentrations throughout the duration of the experiment. A control series was performed in which the only intervention was the infusion of normal saline. In these experiments the maternal and fetal glucose concentrations remained constant as did the volume of distribution of 3-O-methyl glucose in the fetus. The maternal insulin concentration remained constant and fetal insulin concentration changed from 11 +/- 2 microU/ml to 355 +/- 51 microU/ml (P less than 0.01). In the face of this large increase in fetal plasma insulin, there was no change in the placental clearance of 3-O-methyl glucose. In the control condition the clearance was 14.1 +/- 1.0 ml/min per kg and this was 13.8 +/- 1.0 ml/min per kg in the high insulin condition. Fetal insulin may change placental glucose flux by decreasing fetal plasma glucose concentrations but does not do so by changing the activity of the glucose transport system.
Global 4-H Network: Laying the Groundwork for Global Extension Opportunities (2012)

A descriptive study examining 4-H programs in Africa, Asia, and Europe was conducted to provide understanding and direction in the establishment of a Global 4-H Network. Information regarding structure, organizational support, funding, and programming areas was gathered. Programs varied greatly by country, and many partnered with other 4-H organizations around the world. Few content areas offered by the surveyed countries aligned with their major agricultural commodities, even though content areas were available in the United States 4-H Program. The Global 4-H Network has the potential to fill these holes and provide additional opportunities to global programs.