gebhart/patrepeval huggingface dataset:
The intracellular fate of zonula occludens 2 is regulated by the phosphorylation of SR repeats and the phosphorylation/O-GlcNAcylation of S257 (2013)

Zona occludens 2 (ZO-2) has a dual localization. In confluent epithelia, ZO-2 is present at tight junctions (TJs), whereas in sparse proliferating cells it is also found at the nucleus. Previously we demonstrated that in sparse cultures, newly synthesized ZO-2 travels to the nucleus before reaching the plasma membrane. Now we find that in confluent cultures newly synthesized ZO-2 goes directly to the plasma membrane. Epidermal growth factor induces through AKT activation the phosphorylation of the kinase for SR repeats, serine arginine protein kinase 1, which in turn phosphorylates ZO-2, which contains 16 SR repeats. This phosphorylation induces ZO-2 entry into the nucleus and accumulation in speckles. ZO-2 departure from the nucleus requires intact S257, and stabilizing the β-O-linked N-acetylglucosylation (O-GlcNAc) of S257 with O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino-N-phenylcarbamate, an inhibitor of O-GlcNAcase, triggers nuclear exportation and proteosomal degradation of ZO-2. At the plasma membrane ZO-2 is not O-GlcNAc, and instead, as TJs mature, it becomes phosphorylated at S257 by protein kinase Cζ. This late phosphorylation of S257 is required for the correct cytoarchitecture to develop, as cells transfected with ZO-2 mutant S257A or S257E form aberrant cysts with multiple lumens. These results reveal novel posttranslational modifications of ZO-2 that regulate the intracellular fate of this protein.
Predicting Patient Volumes at Collegiate Football Games (2021)

Background: Determining appropriate staffing and resources for mass gathering events (MGEs) such as college football games is challenging. Objective: We sought to create a model predictive of patient volumes at collegiate football games to help aid emergency medical services (EMS) in appropriate health care services planning. Methods: A retrospective review was conducted of patient EMS medical records from 99 Division I collegiate football games played across fourteen football seasons (2005-2018) at one outdoor stadium. A linear regression model with cross validation to the patient illness records was created, using the total number of patients as the outcome measure and variables that can be ascertained prior to game day as the predictors. Results: A formula was derived (R2 = 0.70); predicted number of patients = 1.49 + [5.91 x parking lot hours] + [1.12 x low temperature in F] + [-12.42, if rain=yes] + [18.34, if snow=yes] + [-15.97, if opposing team rank (OTR) is 11-25; -30.48 if OTR is >25] + [-14.50, if home team rank (HTR) is 11-25; -11.52 if HTR is >25]. Conclusion: Weather data, open parking lot hours prior to kickoff, and team rankings are important variables to consider when planning for necessary medical care at collegiate football games.
ChemInform Abstract: Two New Retigerane‐Type Sesterterpenoids from the Lichen Leprocaulon microscopicum. (2016)

Abstract Two new compounds (I) and (II) are isolated, possessing a rare pentacyclic carbon skeleton typical for lichen metabolism, and quite unusual in the vegetal kingdom.
Sipuleucel-T – A Model for Immunotherapy Trial Development (2015)

Historically, immunotherapy has had limited impact in solid tumors with the exception of melanoma and renal cell carcinoma, which have been deemed as “immunologic cancers” given their potential for remissions either spontaneously or following removal of the primary lesion. Despite multiple immunologic approaches with peptide, protein, and DNA vaccines, no one therapy has induced complete remission or maintained durability of response. Sipuleucel-T represents one of several novel immunologic therapy approaches to treat prostate cancer in addition to other solid tumors. It is the first in its class of autologous cellular therapies to demonstrate safety and an overall survival benefit in patients with asymptomatic or minimally symptomatic metastatic castration-resistant prostate cancer and represents a unique treatment approach that may be further enhanced with other biologic agents, chemotherapy, or checkpoint inhibitors. How sipuleucel-T can be used as a foundation on which to build and enhance future clinical trials will be discussed.
The cystic fibrosis transmembrane regulator is present and functional in endosomes. Role as a determinant of endosomal pH. (1992)

Cystic fibrosis is caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR), which lead to defective Cl- conductance in epithelial cells. While the CFTR gene product has been detected in the plasma membrane, its presence and functional role in the membranes of intracellular compartments remain to be established. The purpose of the present experiments was to functionally localize CFTR in the endosomal membrane and to test the role of the associated Cl- conductance in the regulation of endosomal pH (pH(en)). When using conductive protonophores, the net H+ flux across the endosomal membrane of Chinese hamster ovary (CHO) cells is limited by the movement of counterions. Thus, ionic permeability could be estimated indirectly, from the changes in pH(en) determined fluorimetrically. Measurements in situ and in a cell-free microsomal preparation indicate the presence of a protein kinase A (PKA)-activated anion conductance in endosomes from CHO cells transfected with CFTR, but not in endosomes from wild-type or mock-transfected cells. In endosomes isolated from CFTR-expressing cells, the stimulatory effect of PKA was diminished by a specific peptide inhibitor of PKA, by alkaline phosphatase treatment or by a monoclonal antibody against the second nucleotide binding fold of CFTR. Increasing counterion permeability by phosphorylation of CFTR or by addition of valinomycin failed to alter the rate or extent of endosomal acidification in situ. Our observations indicate that functional CFTR, susceptible to activation by PKA, is present in endosomes of transfected CHO cells. More importantly, the data suggest that factors other than counterion permeability are the major determinants of pH(en).
Aryl Diazonium versus Iodonium Salts: Preparation, Applications and Mechanisms for the Suzuki–Miyaura Cross‐Coupling Reaction (2011)

Abstract This review gives an overview of the use of unusual diazonium and iodonium salts as electrophiles for the Suzuki–Miyaura reaction. The discussion mainly focuses, with a critical view, on catalytic systems developed with these electrophiles. The review also discusses their general properties and preparation as well as mechanistic aspects. A last and brief comparison of diazonium versus iodonium salts, by highlighting advantages and drawbacks of both, gives the reader an understandable resumé.
Reconfigurable biconcave lens antenna based on plasma technology (2023)

This article is focused on the application of plasma technology for the development of microwave lens antennas with electronically controllable radiation gain. With this aim, the analytical background and design procedure for designing a biconcave lens based on plasma dielectric material are presented. The procedure is used to design a plasma lens antenna with a pyramidal horn feed. The effect of switching the designed lens ON and OFF on the radiation gain of the lens antenna is investigated. It is also shown that the plasma frequency of the lens can be used to dynamically adjust the radiation gain. A one-dimensional version of the proposed plasma lens operating at 10 GHz has been developed to validate the concept. Experimentally measured characteristics of a fabricated prototype of the lens antenna based on commercially available fluorescent lamps confirm the presented design procedure and numerical results. The results also show that changing the plasma frequency of the lens can be used to adjust the radiation gain of the proposed lens antenna.
Coded asynchronous CDMA and its efficient detection (1998)

In this paper, receiver design and performance analysis for coded asynchronous code-division multiple access (CDMA) systems is considered. The receiver front-end consists of the near-far resistant multiuser detector known as the projection receiver (PR). The PR performs multiple-access interference resolution and is followed by error-control decoding. The output of the projection receiver yields the appropriate metric (i.e., soft information) for decoding of the coded sequences. An expression for the metric is derived that allows the use of a standard sequence decoder (e.g., Viterbi algorithm, M-algorithm) for the error-control code. It is then shown that the metric computer has an elegant adaptive implementation based on an extension of the familiar recursive least squares (RLS) algorithm. The adaptive PR operates on a single sample per chip and achieves a performance virtually identical to the algebraic PR, but with significantly less complexity. The receiver performance is studied for CDMA systems with fixed and random spreading sequences, and theoretical performance degradations with regard to the single-user bound are derived. The near-far resistance of the PR is also proven, and demonstrated by simulation.
The Impact of Shallow-Trench-Isolation Mechanical Stress on the Hysteresis Effect of Partially Depleted Silicon-on-Insulator n-Type Metal-Oxide-Semiconductor Field Effects (2014)

The impact of shallow trench isolation (STI) mechanical stress on the hysteresis effect in the output characteristics is measured in partially depleted (PD) silicon-on-insulator (SOI) metal-oxide-semiconductor field effect transistors (MOSFETs). We develop ID hysteresis, which is defined as the difference between ID versus VD forward sweep and reverse sweep. The fabricated devices show positive and negative peaks in ID hysteresis. The experimental results show that ID hysteresis declined as the STI mechanical stress increases. We also elaborate on the impact of STI mechanical stress on the ID hysteresis of PD SOI n-type MOSFETs.
SEDACS-a client/server approach to TPS development (2002)

The C-17 Support Equipment Data Acquisition and Control System (SEDACS) Test Program Set/Test Requirements Document (TPS/TRD) development system was upgraded from a host-based Honeywell/Multics mainframe system to a new client/server system with Internet connectivity. Reliability, flexibility, and supportability were the requirements for the new system. The combination of the client/server model and commercial software met these requirements by exploiting fast and inexpensive hardware and commercial off-the-shelf (COTS) software such as word processing and project and circuit analysis software. Greater efficiencies were realized by reducing the required time needed to train users, develop TPSs, and prepare supporting documentation. Quality was improved by incorporating configuration management tools and integrated spell checking into the applications suite and by designing around a centralized database. This paper briefly describes how we developed our new system and how we migrated from our existing mainframe (or legacy) system to a client/server system.
Automated Camera-Based Estimation of Rehabilitation Criteria Following ACL Reconstruction (2018)

Anterior cruciate ligament (ACL) reconstruction necessitates months of rehabilitation, during which a clinician evaluates whether a patient is ready to return to sports or occupation. Due to their time- and cost-intensive nature, these screenings to assess progress are unavailable to many. This paper introduces an automated, markerless, camera-based method for estimating rehabilitation criteria following ACL reconstruction. To evaluate the performance of this novel technique, data were collected weekly from 12 subjects as they used a leg press over the course of a 12-week rehabilitation period. The proposed camera-based method for estimating displacement and force was compared to encoder and force plate measurements. The leg press displacement and force values were estimated with 89.7% and 85.3% accuracy, respectively. These values were then used to calculate lower-limb symmetry and to track patient progress over time.