gebhart/patrepeval huggingface dataset:
A solid-phase fluorescent immunoassay for human prostatic acid phosphatase. (1978)

Abstract Prostatic acid phosphatase was extracted from human malignant prostatic tissues by Tween 80 and precipitated with 40 to 75% of ammonium sulfate. The crude extract of prostatic acid phosphatase was further purified by a series of chromatographies with concanavalin A-Sepharose, diethylaminoethyl cellulose, and Sephadex G-100. A homogeneous prostatic acid phosphatase preparation with an 85-fold purification and a recovery of 38% of initial enzyme activity was obtained. Antiserum was raised by immunizing female rabbits with the purified enzyme preparation. The anti-prostatic acid phosphatase serum did not cross-react with the acid phosphatase of other human tissues. The anti-prostatic acid phosphatase immunoglobulin G (IgG) antibody was isolated by diethylaminoethyl cellulose and conjugated to CNBr-activated Sepharose 4B, which was then used in a solid-phase fluorescent immunoassay for serum prostatic acid phosphatase. Prostatic acid phosphatase in the serum was separated from other acid phosphatases by mixing serum with antiprostatic acid phosphatase (IgG)-Sepharose 4B; the enzyme activity was subsequently measured by incubating prostatic acid phosphatase-IgG-Sepharose and α-naphthyl phosphate. The hydrolysis product, α-naphthol, is fluorogenic and can be quantitated with a spectrophotofluorometer at 340 nm (excitation) and 465 nm (emission). The sensitivity of this solid-phase immunofluorometric assay was 60 pg of prostatic acid phosphatase per ml, more sensitive than other immunoassays for prostatic acid phosphatase. Further, the antibody could be reused after the prostatic acid phosphatase was dissociated from IgG-Sepharose 4B. Normal male serum was found to contain an average of 5.619 ng of prostatic acid phosphatase per ml (from 30 normal males, range 1.399 to 9.839 ng per ml). Patients with nonprostatic cancer have normal serum prostatic acid phosphatase levels. Patients with early stages of prostate tumor had elevated levels of serum prostatic acid phosphatase.
Fuzzy Vault Fingerprint Smartcard Implementation Using an Orientation-Based Feature Vector (2008)

With the increasing employment of biometric authentication systems worldwide, there has been significant concern over the security of stored biometric templates. In this paper, a solution to this problem employing a fuzzy vault fingerprint smartcard implementation, unlocked via an orientation-based feature vector, is presented. The fuzzy vault contains an unknown key which can be used either for identification or for file encryption/decryption.
Phenolic and bis-iridoid glycosides from Strychnos cocculoides (2009)

A new bis-iridoid glucoside, cocculoside (1), has been isolated, together with five known compounds from Strychnos cocculoides (Loganiaceae), collected in Tanzania. The structure of cocculoside (1) has been determined as an ester dimer of loganin and secologanin dimethyl acetal between C-7 and C-11.
FVTD Modeling of a Localized Microwave Plasma Discharge in Microstrip Wave Guide (2011)

International audience
Fail-Safe Requirements for Holding Tube Pressure in an Ultra-High-Temperature Steam Injection System (1980)

A steam injection system for ultra-hightemperature pasteurization was examined for fail-safe requirements for pressure within the holding tube.
Micro-architecture independent branch behavior modeling (2015)

In this paper, we propose linear branch entropy, a new metric for characterizing branch behavior. The metric is independent of the configuration of a specific branch predictor, but it is highly correlated with the branch miss rate of any predictor. In particular, we show that there is a linear relationship between linear branch entropy and the branch miss rate. This means that the metric can be used to estimate branch miss rates without simulating a branch predictor by constructing a linear function between entropy and miss rate. The resulting model is more accurate than previously proposed branch classification models, such as taken rate and transition rate. Furthermore, linear branch entropy can be used to analyze the branch behavior of applications, independent of specific branch predictor implementations, and the linear branch miss rate function enables comparing branch predictors on how well they perform on easy-to-predict versus hard-to-predict branches. As a case study, we find that the winner of the latest branch predictor competition performs worse on hard-to-predict branches, compared to the third runner-up; however, since the benchmark suite mainly consisted of easy branches, a predictor that performs well on easy-to-predict branches has a lower average miss rate.
T4 ARE WE IN THE DARK ABOUT INJECTING? (2005)

Nuclear Medicine Dept, Grampian University Hospitals, Aberdeen, UK Abstracts of the spring 2005 meeting of the British Nuclear Medicine Society Manchester International Convention Centre, UK; 14–16 March, 2005
Assessment of the Relative Contribution of Different Protease Inhibitors to the Inhibition of Plasmin In Vivo (1993)

It has been shown that the most important inhibitor of plasmin is alpha 2-antiplasmin, however, other protease inhibitors are able to inhibit this proteolytic enzyme as well. The contribution of the various protease inhibitors to the inhibition of plasmin in vivo has never been quantitatively assessed. To assess the relative contribution of the different protease inhibitors on the inhibition of plasmin we developed a series of sensitive immunoassays for the detection of complexes between plasmin and the protease inhibitors alpha 2-antiplasmin, alpha 2-macroglobulin, antithrombin III, alpha 1-antitrypsin and C1-inhibitor, utilizing monoclonal antibodies that are specifically directed against complexed protease inhibitors and a monoclonal antibody against plasmin. It was confirmed that alpha 2-antiplasmin is the most important inhibitor of plasmin in vivo, however, complexes of plasmin with alpha 2-macroglobulin, antithrombin III, alpha 1-antitrypsin- and C1-inhibitor were also detected. Particularly during activation of fibrinolysis complexes between plasmin and inhibitors other than alpha 2-antiplasmin were detected. It was observed that during different situations the inhibition profile of plasmin was not constant e.g. in patients with diffuse intravascular coagulation plasma levels of plasmin-alpha 1-antitrypsin and plasmin-C1-inhibitor were increased whereas in plasma from patients who were treated with thrombolytic agents complexes of plasmin with alpha 2-macroglobulin and with antithrombin III were significantly elevated. In conclusion, we confirmed the important role of alpha 2-antiplasmin in the inhibition of plasmin, however, in situations in which fibrinolysis is activated other protease inhibitors also account for the inhibition of plasmin in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)
Silicon Ingot Quality and Resulting Solar Cell Performance (2013)

This paper demonstrates that the electronic properties of a multicrystalline solar ingot can be used to predict the performance of solar cells manufactured from it. The lifetime and trap density are measured on as-cut bricks and are used to define a single metrological parameter termed "Q-Factor". The Q-Factor scales with the performance of over a dozen ingots cast from experimental Solar-Grade Silicon. It has the unit of a lifetime and relates to the minority carrier lifetime at cell level. It is argued that the measurement of electronic properties of Silicon at brick level is a sensitive probe for contaminations and can be considered as complementary to a chemical analysis. It can predict the performance potential of the brick with the added advantage of being sensitive to the crystallization quality.
Cyclical Electrical Stimulation of Hydrogel Microactuators Employing Parylene-N Coated Electrodes (2013)

This work presents the cyclical actuation of electric field sensitive microscale hydrogels employing dielectric coated coplanar electrodes. Microscale hydrogels are photopolymerized in-situ, and AC frequency-based actuation combined with pulse width modulation enabled controlled manipulation of hydrogel deformation. Stable actuation cycles are achieved with applied electric potentials from 20 V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">pk-pk</sub> to 40 <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Vpk-pk</sub> , with a maximum true strain of 29% and a minimum rise time of 4.7 s. The peak and trough osmotic pressure for each system' s cycle is also analytically determined, with a peak pressure at 40 V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">pk-pk</sub> of 201.1±38.3 kPa. A plateau in the peak-to-trough true strain is observed above 30 V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">pk-pk</sub> . For comparative purposes a system without dielectric coated electrodes and employing external syringe pumps is also examined, and stable cyclical actuation was achieved for applied electric potentials of 5 V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">pk-pk</sub> and 10 V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">pk-pk</sub> . For this system the maximum stable rise time, true strain, and osmotic pressure are 8.1 s, 57%, and 429.2±81.9 kPa, respectively. The difference between the two systems highlights how optimization of the dielectric layer's thickness and uniformity can further enhance actuation performance. The electronically responsive hydrogel-based cyclical actuator developed within this work could be further employed for microfluidic regulation in portable low-power systems.
Controllability of Pore Size and Porosity on Self-Organized Porous Silica Particles (2002)

Silica powder containing organized pores was prepared by a spray drying method. Silica and polystyrene latex (PSL) nanoparticles colloids were mixed and atomized to form micrometer-sized droplets. Nitrogen carrier gas was used to carry the resulting droplets into a vertical reactor that contained two heating zones: 200 °C and 450 °C, which were used, respectively, to evaporate the dispersing medium (water) and to decompose the PSL particles to give a porous silica powder. The pores on the surface of the powders were found to be arranged into a hexagonal packing, indicating that a self-organization process occurred spontaneously during evaporation of the solvent. The pore size was controlled by changing the size of the PSL particles. By adding an additional zone (third zone) maintained at high temperatures, the produced powders could be in-situ annealed. A comparison of the average volume of the powder before and after annealing (at 1500 °C) indicated that the porosity of the powder was about 70%. Together with the TEM image, this result indicated that pores were present both on the surface of the powders, as well as, inside the powder particles.
Characterization of a Legionella pneumophila extracellular protease exhibiting hemolytic and cytotoxic activities (1989)

A preliminary screening of selected Legionella species for proteolytic and hemolytic phenotypes suggested a correlation between these activities. To investigate the relationship of these phenotypes, a mutant strain of Legionella pneumophila deficient in the expression of a 38-kilodalton (kDa) exoprotease was isolated and characterized. This strain, designated PRT8, was also found to be nonhemolytic when screened on blood agar composed of 5% canine or guinea pig erythrocytes. Strain PRT8 was serologically and biochemically identical to the parental strain with the exception of the expression of the exoprotease. Immunoblot analysis of concentrated culture filtrates from PRT8 probed with polyclonal anti-38-kDa exoprotease serum revealed no cross-reactive peptides. To resolve the role of the exoprotease in the hemolytic phenotype, the exoprotease was purified from the culture supernatant of the parental strain by a combination of ion-exchange and hydrophobic interaction chromatography steps. The hemolytic activity was found to copurify with the proteolytic activity, and analyses by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot revealed a single protein species exhibiting an apparent molecular mass of 38 kDa. Finally, the purified exoprotease and concentrated culture supernatant from the parental strain, but not from PRT8, exhibited cytotoxicity (minimum cytotoxic activity of 0.17 U of protease activity) in a Chinese hamster ovary cell assay. These data suggest that the exoprotease is responsible for the hemolytic and cytotoxic phenotypes described for this species and therefore may be one of several determinants associated with virulence.
Solar-driven, highly sustained splitting of seawater into hydrogen and oxygen fuels (2019)

Electrolysis of water to generate hydrogen fuel is an attractive renewable energy storage technology. However, grid-scale freshwater electrolysis would put a heavy strain on vital water resources. Developing cheap electrocatalysts and electrodes that can sustain seawater splitting without chloride corrosion could address the water scarcity issue. Here we present a multilayer anode consisting of a nickel-iron hydroxide (NiFe) electrocatalyst layer uniformly coated on a nickel sulfide (NiSx) layer formed on porous Ni foam (NiFe/NiSx-Ni), affording superior catalytic activity and corrosion resistance in solar-driven alkaline seawater electrolysis operating at industrially required current densities (0.4 to 1 A/cm<sup>2</sup>) over 1,000 h. A continuous, highly oxygen evolution reaction-active NiFe electrocatalyst layer drawing anodic currents toward water oxidation and an in situ-generated polyatomic sulfate and carbonate-rich passivating layers formed in the anode are responsible for chloride repelling and superior corrosion resistance of the salty-water-splitting anode.
Multi-feature and DAG-Based Multi-tree Matching Algorithm for Automatic Web Data Mining (2014)

Web data extraction has received considerable attention and study in recent decades. To improve efficiency, many automatic Web data record mining approaches have been proposed. Among these approaches, each complete approach involves data record identification as well as data item alignment. In this paper, we propose a new multi-feature and DAG (Directed Acyclic Graph) based multi-tree matching algorithm for automatic data item alignment. Our algorithm improves alignment accuracy in two aspects. First, it combines multiple features to cope with the limitations of existing algorithms, second, it employs a DAG-based method to deduce the global alignment of data items with high accuracy. Experimental results show that our algorithm outperforms state-of-the-art data item alignment algorithms.
ChemInform Abstract: THIN FILM CADMIUM SELENIDE ELECTRODEPOSITED FROM SELENOSULFATE SOLUTION (1985)

Abstract CdSe‐Filme auf Ti wurden durch Reduktion einer wäßrigen Lösung mit SeSOä′ ‐Anionen [aus der Auflösung von Se‐Pulver in 0.6 M Na‐sulfit‐Lösung bei pH 9] und einem Cd‐Komplex [aus der Auflösung von CdClg ‐ 2.5 H 2 O in wäßriger 0.103 M Na‐nitrilotriacetat‐Lösung bei pH 8] erhalten (l h Elektrolyse mit Ti‐Kathode).
Far away from the lamppost (2018)

This Formal Comment responds to a recent Meta-Research Article by identifying initiatives that are already in place for funding risky exploratory research that illuminate mysteries of the dark genome.
Oblique Transcorporeal Drilling to Treat Anterior Compression of the Spinal Cord at the Cervical Level (1994)

Oblique transcorporeal drilling is a new surgical technique in which the transverse foramina with the vertebral artery and lateral aspects of the bodies of the cervical vertebrae are exposed; it allows to drill out obliquely from the antero-lateral to the opposite postero-lateral corner, half of one or several cervical vertebral bodies. The technique is shortly described and its indications are discussed. It can mainly be applied to release osteophytic compression and to remove anteriorly developed tumors. The advantages are to work in a wide field with all important structures kept medially and protected and to keep a sufficient portion of the vertebral bodies to preserve the spine stability. This technique has been used in 39 cases with spondylosis in 27 cases, tumor in 11 cases and kyphosis in 2 cases including one with neurofibroma. Results in term of decompression were excellent in all cases. Mortality was none and morbidity was limited to a transient Horner's syndrome in almost all cases and to a CSF leak in three cases. This technique is therefore alternative to the anterior transcorporeal approach. In our experience, the oblique transcorporeal drilling provides a wider and safer access to lesions located anterior to the spinal cord.
Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318‐knockout mice (2016)

Zfp318, a mouse gene with a Cys2/His2 zinc finger motif, is mainly expressed in germ cells in the testis. It encodes two alternative transcripts, which regulate androgen receptor-mediated transcriptional activation or repression by overexpression of them. However, the role of Zfp318 is still obscure in vivo, especially in spermatogenesis. To elucidate the role of Zfp318 during gamete production, we established a knockout mouse line. Zfp318-null male mice exhibited infertility, whereas Zfp318-null female mice displayed normal fertility. ZFP318 was expressed during multiple stages of spermatogenesis, from spermatocytes to round spermatids. The nuclei of secondary spermatocytes showed high levels of expression. Histological analysis and quantitative analysis of DNA content showed decreased numbers of both spermatids in the seminiferous tubules and mature spermatozoa in the epididymides of Zfp318-null mice. These results suggest that Zfp318 is expressed as a functional protein in testicular germ cells and plays an important role in meiosis during spermatogenesis.
LB1.3 Anticipating resistance ofmycoplasma genitaliumto quinolones and macrolides: nanjing, china (2017)

<h3>Introduction</h3> For treatment of <i>Mycoplasma genitalium</i> (Mg) infection, azithromycin is first line initial treatment but persistent NGU caused by Mg may not respond to repeat azithromycin treatment; quinolones, in particular moxifloxcin, have been shown to be effective. The molecular epidemiology of mutations in macrolide- and quinolone-resistance determining regions (RDRs) of 23S rRNA, parC and gyrA genes remains unclear in China. <h3>Methods</h3> From April 2011 to August 2015, male subjects with clinical and microscopic evidence of urethritis provided a first-voided urine specimen and the mgpA gene was partially amplified and sequenced to diagnose Mg infection. RDRs of the 23s rRNA, parC and gyrA genes were sequenced. <h3>Results</h3> Among 1816 male-patients, the overall prevalence of<i> M. genitalium</i> was 19.7% (358/1816). <i>N. gonorrhoeae</i> prevalence was 46.6% (847/1816). Among 969 NGU-cases, prevalence of: <i>C. trachomatis</i> was 42.3% (409/968), <i>M. genitalium</i> 27.9% (271/969), <i>U. urealyticum</i> 21.3% (182/856), <i>T. vaginalis</i>1.8% (17/969) and <i>M. hominis</i> 0.7% (6/855). Based on data available from partial (5.4%) sequencing of the MgPa gene (mgpA) an evolutionary tree was constructed that divided the 358 <i>Mg</i> mgpA sequences into five major clusters. Among 358 <i>Mg</i> positive samples, successful sequencing was accomplished for: the 23s rRNA gene (341 specimens), the parC gene (344 specimens) and the gyrA gene (339 specimens). 88.9% (303/341) had mutations in 23s rRNA, 89.5% (308/344) had sense mutations in parC, and 12.4% (42/339) had sense mutations in gyrA.The most common single base pair mutation in the 23s rRNA gene was A2059G (211/303; 69.6%) followed by A2058G (60/303; 19.8%) and A2059T (30/303; 9.9%). The most common mutation in the parC gene was G248T (289/344; 84%) and in the gyrA gene G285A (22/339, 6.5%). <h3>Conclusion</h3> The high mutation rate in 23s rRNA and parC in Mg strains in Nanjing are a harbinger of resistance to macrolides and quinolones that may follow. Molecular surveillance of indigenous strains of Mg is warranted to anticipate the development of antimicrobial resistance.
ATPS-68COMBINATION OF TOPOISOMERASE II POISON BERUBICIN WITH STAT3 INHIBITOR WP1066BLOCKS POTENTLY TUMOR GROWTH IN GMB MODELS (2015)

BACKGROUND: Berubicin (WP744) is a DNA binding agent and potent topoisomerase II poison designed and synthesized in our laboratory. Preclinical studies demonstrated that WP744 is a potent anticancer agent with nanomolar potency in glioma cells. WP744 was shown to effectively cross blood-brain barrier (BBB) and displayed antitumor activity in orthotopic glioma models. WP744 gained IND approval and subsequent Phase I clinical trials shows clinical benefits (including complete response) in selected brain tumor patients. Signal Transducer and Activator of Transcription 3 (STAT3) is an oncogenic transcription factor that is activated in numerous tumors including GBM. WP1066, an analog of caffeic acid benzyl ester (CABE, a component of propolis), is a potent inhibitor of activated STAT3 (STAT3-Y705) designed in our laboratory. WP1066 is currently undergoing preclinical development and has demonstrated anticancer activity in multiple cancer models including GBM. WP1066 similarly to WP744 is able to cross BBB. The goal of this study is to assess therapeutic effects of WP744 in combination with WP1066 in the in vitro and in vivo GBM models. RESULTS: WP744 and WP1066 were tested in a panel of GBM cell lines including GSC cell lines. Both, WP744 and WP1066, effectively induce apoptosis and inhibit cells growth at low nanomolar and micromolar concentrations, respectively. Dosing schedule for a combination in vivo therapy was tested in CD-1 mice. The proof-of-principle efficacy study was performed in subcutaneous U87 GBM tumor model and demonstrated superior tumor growth inhibition in a group receiving combination of WP1066 and WP744. CONCLUSION: Proposed novel therapeutic strategy involving two compounds (WP744 and WP1066) with significant CNS uptake that are mechanistically complementary is highly promising and our results warrant further evaluation in different brain tumor models. Consistent activity of WP744 combination with WP1066 in a broader range of tumors would open possibility for its future clinical evaluation.
MOCVD of SnO2 thin films from a new organometallic precursor (1999)

The present work deals with the use and advantages of diethylaminodimethylstannane(IV) (I) as precursor for the CVD of SnO 2 thin films. The films are deposited on α-Al 2 O 3 and SiO 2 /Si(100) in O 2 +H 2 O atmospheres, at temperatures langing from 400 to 500°C. High-purity, nanocrystallme layers with (101) preferential orientation are always obtained, A sovel procedure for doping SnO 2 films with RuO 2 , which consists in a chemical bath deposition on tin dioxide layers, is also described. Particular emphasys is given to the possibility of tailoring material properties by a proper choice of the synthesis conditions. The thermal decomposition pattern of the precursor (I) is studied by an 1 H-NMR analysis of its pyrolysis byproducts. The microstructure of the samples is investigated by XRD, while their surface and in-depth chemical compositior is studied by XPS. The morphology of the films and its dependance on the nature of the growth surface is exammed by AFM Electrieal measurements indicate that RuO 2 introduction in the SnO 2 host matrix induces an appreciable enhancement of the ayer conductivity.