gebhart/patrepeval huggingface dataset:
(Invited) Photostability of Core-Shell Structures at Low Temperature (2020)

The use of carbon nanotubes as single emitters is currently developing. Being made only of surface atoms carbon nanotubes are very sensitive to their environment. A fingerprint of this sensitivity is the variation with time of their emission intensity (blinking) and energy (spectral jitter) in low temperature experiments. To improve the stability of nanotubes, we have synthesized core-double shell structures where two shells of polymers are rolled around the nanotube [1]. Here, we will show the influence of these shells on the stability of both the emission intensity and energy [2]. [1] L. Orcin-Chaix et al, Journal of Material Chemistry C 6, 4786 (2018) [2] L. Orcin-Chaix et al, in preparation
Genetic variation in an inbred plant: variation in tissue cultures of soybean [Glycine max (L.) Merrill]. (1989)

Although soybean [Glycine max (L.) Merrill] grows as an inbreeding, generally homozygous, plant, the germplasm of the species contains large amounts of genetic variation. Analysis of soybean DNA has indicated that variation of RFLP (restriction fragment length polymorphism) markers within the species usually entails only two alleles at any one locus and that mixtures of such dimorphic loci account for virtually all of the restriction fragment variation seen in soybean (G. max), and in its ancestors, G. soja and G. gracilis. We report here that tissue cultures prepared from root tissue of individual soybean plants develop RFLP allelic differences at various loci. However, these newly generated alleles are almost always the same as ones previously found and characterized in other varieties of cultivated soybean (cultivars). This repeated generation of particular alleles suggests that much of the genetic variation seen in soybean could be the consequence of specific, relatively frequently employed, recombinational events. Such a mechanism would allow inbred cultivars to generate genetic variation (in the form of alternative alleles) in a controlled manner, perhaps in response to stress.
Studies on the Role of Uncharged tRNA in Pleiotypic. Response of Animal Cells (1976)

Experiments were carried out to assess the physiological significance of the charging level of tRNA. Histidinol, a competitve inhibitor of charging of tRNAHis, was used to induce uncharged tRNA in mammalian cells. It is demonstrated that both in the presence of histidinol and under histidine depletion about 40% of the tRNAHis is uncharged. Concomitant with this appearance of uncharged tRNA(a) the pools of GTP and ATP are decreased rapidly by 25--30%; (b) the synthesis of both protein and ribosomal RNA is inhibited, whereas that of nucleoplasmic RNA is not affected; (c) the uptake of 2-deoxyglucose, phosphate, Ca2+; uridine and adenosine is inhibited; and (d) the growth of 3T6 fibroblasts is arrested. It is suggested that the appearence of uncharged tRNA is one of the earliest events occurring under conditions of amino acid starvation, which in turn causes the various metabolic changes observed.
ChemInform Abstract: CYCLOADDITION REACTIONS OF 1,3‐DIACETYLIMIDAZOLIN‐2‐ONE (1981)

Abstract 1,3‐Diacetyl‐2‐imidazo‐ linon (III) reagiert mit den Dienen (II) bzw. Anthracen (IV) im Sinne einer Diels‐ A1der‐Reaktion zu den Addukten (I) bzw. (V).
Chemical composition and anti-arthritic activity of Anacyclus valentinus extract on adjuvant-induced arthritis in rats (2017)

Anacyclus valentinus L. is a common annual plant in Algeria, known for her various therapeutic effects. In addition, the plant is used as a food condiment. We reported our investigations on the chemical compositions and the antiarthritic activity of methanolic extract of A. valentinus (MEAV). The polyphenol extraction by maceration with methanol (80%) gave yields of 17.82%. The identification by LC-MS and colorimetric assays revealed the wealth of methanolic extracts on phenolic compounds including flavonoids (52.15 mg Equ/g) and lactones. Acute oral toxicity of extract was performed in line with OECD guidelines and the lethal dose 50 was assessed greater than 2500 mg/Kg. Regarding the anti-arthritic power, rheumatoid arthritis was induced by Freund's adjuvant in rats. The methanolic extract of A. valentinus presented a largest effect with weight gain, an arthritic score, thymus indices; spleen and serum parameters close to those of the control. The extract also inhibited edema and restored cartilage structure.
Protein Adsorption to Charged Gold Nanospheres as a Function of Protein Deformability (2017)

The corona that forms as protein adsorbs to gold nanospheres (AuNSs) is directly influenced by the surface chemistry of the AuNS. Tools to predict adsorption outcomes are needed for intelligent design of nanomaterials for biological applications. We hypothesized that the denaturation behavior of a protein might be a useful predictor of adsorption behavior to AuNSs, and used this idea to study protein adsorption to anionic citrate-capped AuNSs and to cationic poly(allylamine hydrochloride) (PAH) wrapped AuNSs. Three proteins (α-amylase (A-Amy), β-lactoglobulin (BLG), and bovine serum albumin (BSA)), representing three different classes of acid denaturation behavior, were selected with BLG being the least deformable and BSA being the most deformable. Protein adsorption to AuNSs was monitored via UV-vis spectrophotometry and dynamic light scattering. Changes to the protein structure upon AuNS interaction were monitored via circular dichroism spectroscopy. Binding constants were determined using the Langmuir adsorption isotherm, resulting in BSA > BLG ≫ A-Amy affinities for citrate-capped gold nanospheres. PAH-coated AuNSs displayed little affinity for these proteins at similar concentrations as citrate-coated AuNSs and became agglomerated at high protein concentrations. The enzymatic activity of A-Amy/citrate AuNS conjugates was measured via colorimetric assay, and found to be 11% of free A-Amy, suggesting that binding restricts access to the active site. Across both citrate AuNSs and PAH AuNSs, the changes in secondary structure were greatest for BSA > A-Amy > BLG, which does follow the trends predicted by acid denaturation characteristics.
Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis (2003)

Nucleobases and derivatives like cytokinins and caffeine are translocated in the plant vascular system. Transport studies in cultured Arabidopsis cells indicate that adenine and cytokinin are transported by a common H+-coupled high-affinity purine transport system. Transport properties are similar to that of Arabidopsis purine transporters AtPUP1 and 2. When expressed in yeast, AtPUP1 and 2 mediate energy-dependent high-affinity adenine uptake, whereas AtPUP3 activity was not detectable. Similar to the results from cell cultures, purine permeases (PUP) mediated uptake of adenine can be inhibited by cytokinins, indicating that cytokinins are transport substrates. Direct measurements demonstrate that AtPUP1 is capable of mediating uptake of radiolabeled trans-zeatin. Cytokinin uptake is strongly inhibited by adenine and isopentenyladenine but is poorly inhibited by 6-chloropurine. A number of physiological cytokinins including trans- and cis-zeatin are also efficient competitors for AtPUP2-mediated adenine uptake, suggesting that AtPUP2 is also able to mediate cytokinin transport. Furthermore, AtPUP1 mediates transport of caffeine and ribosylated purine derivatives in yeast. Promoter-reporter gene studies point towards AtPUP1 expression in the epithem of hydathodes and the stigma surface of siliques, suggesting a role in retrieval of cytokinins from xylem sap to prevent loss during guttation. The AtPUP2 promoter drives GUS reporter gene activity in the phloem of Arabidopsis leaves, indicating a role in long-distance transport of adenine and cytokinins. Promoter activity of AtPUP3 was only found in pollen. In summary, three closely related PUPs are differentially expressed in Arabidopsis and at least two PUPs have properties similar to the adenine and cytokinin transport system identified in Arabidopsis cell cultures.
Limited clinical value of exhaled volatile organic compound measurements in childhood asthma (2018)

<b>Exhaled volatile organic compound measurements do not aid the clinician diagnosing asthma in children</b> http://ow.ly/Z2d930lpZ60.
Radiation characteristics of cylindrical dielectric resonator antennas with new applications (1989)

Last fall (1987), I was invited to give lectures at the Institute of Electromagnetic Radiation, Shanghai University of Technology (SUT), in China. It was a series of unforgettable, deeply impressive experiences for me (photo 1). Vice Director of the Institute, Professor Shuozhong Wang, published the Institute's progress in the Newsletter of Shanghai, Gongye Daxue, dated December 20, 1987. Of course, it is written in Chinese; Professor Wang translated into English as follows.
Image artifacts in digital breast tomosynthesis: Investigation of the effects of system geometry and reconstruction parameters using a linear system approach (2008)

Digital breast tomosynthesis (DBT) is a three-dimensional (3D) x-ray imaging modality that reconstructs image slices parallel to the detector plane. Image acquisition is performed using a limited angular range (less than 50 degrees) and a limited number of projection views (less than 50 views). Due to incomplete data sampling, image artifacts are unavoidable in DBT. In this preliminary study, the image artifacts in DBT were investigated systematically using a linear system approximation. A cascaded linear system model of DBT was developed to calculate the 3D presampling modulation transfer function (MTF) with different image acquisition geometries and reconstruction filters using a filtered backprojection (FBP) algorithm. A thin, slanted tungsten (W) wire was used to measure the presampling MTF of the DBT system in the cross-sectional plane defined by the thickness (z-) and tube travel (x-) directions. The measurement was in excellent agreement with the calculation using the model. A small steel bead was used to calculate the artifact spread function (ASF) of the DBT system. The ASF was correlated with the convolution of the two-dimensional (2D) point spread function (PSF) of the system and the object function of the bead. The results showed that the cascaded linear system model can be used to predict the magnitude of image artifacts of small, high-contrast objects with different image acquisition geometry and reconstruction filters.
Electronic Excited States (2001)

Chapter 25 examines an array of techniques for describing electronic excited states. The relative merits of these techniques are discussed.
Design of Potentially Photorefractive Liquid Crystalline Materials:  Derivatives of 3,6-Disubstituted Carbazole (2005)

The incorporation of photorefractive molecular units, such as carbazole, into anisotropic materials (liquid crystals) may offer many advantages over conventional electrical poling of photorefractive polymers. Thus, a series of symmetric 3,6-disubstituted carbazole derivatives with a bent molecular shape have been synthesized and characterized. The influence of the nature of the symmetric substituents on the carbazole is investigated through comparison of crystal packing and electrostatic potentials.
The rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP (2013)

The rotational order-disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order-disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.
Iatrogenic Type A Dissection: Case Series and Surgical Management From a High-Volume Aortic Center (2023)

Iatrogenic type A dissections represent a rare but serious complication of cardiac procedures, with high in-hospital mortality for those undergoing surgical repair. A repair strategy involving an open distal anastomosis and proximal root reconstruction ensures durable freedom from need for redo surgery.
Selective steam injection in open-hole, gravel-packed, liner compositions (1976)

During steam soak or steam drive operations to improve offtake from heavy oil reservoirs, injection profiles are often poor resulting in reduced recoveries. This work describes a field proven method for the selective injection of steam in slotted liner completions gravel-packed in the under-reamed production interval. The liner pack-off is attained by incorporating polished nipples in the liner make-up and using matching mandrels equipped with a friction ring hold-down on the injection string. The gravel-pack is plugged by squeezing a mixture of Am-9, bentonite and cement through a port collar set in a liner blank section.
Cell motility, a principal requirement for metastasis (1991)

In studying the role of motility in the metastasis of tumor cells, we have described an autocrine motility factor. This agent, which stimulates random motility, probably contributes to the initial dissociation of the cells from the primary tumor mass. Extracellular matrix components, via several different mechanisms, may facilitate the crossing of biological barriers by the cells prior to the entry into the circulation. In locating at new sites, the tumor cells may be induced to exit from the circulation in response to attractants such as IGFs that could emanate from the target organ. These same growth factors could then stimulate cellular proliferation for another metastatic cycle. It is quite probable that detection of AMF may provide a new tool in cancer diagnosis. The complete characterization of AMF may also yield valuable therapeutic approaches: design of low molecular size antagonists of the attractants and antibodies that might be effective therapeutically as well as diagnostically. It seems clear, in any event, that immobilizing the tumor cell may be a crucial step in inhibiting metastasis.
Reduction factors for creep strength and fatigue life of modified 9 Cr-1 Mo steel weldments (1991)

The provisions of ASME B PV Code Case N-47 currently include reduction factors for creep strength and fatigue life of weldments. To provide experimental confirmation of such factors for modified 9 Cr-1 Mo steel, tests of tubular specimens were conducted at 538{degree}C (1000{degree}F). Three creep-rupture specimens with longitudinal welds were tested in tension; and, of three with circumferential welds, two were tested in tension and one in torsion. In each specimen with a circumferential weld, a nonuniform axial distribution of strain was easily visible. The test results were compared to an existing empirical model of creep-rupture life. For the torsion test, the comparison was based on a definition of equivalent normal stress recently adopted in Code Case N-47. Some 27 fatigue specimens, with longitudinal, circumferential, or no welds, were tested under axial or torsional strain control. In specimens with welds, fatigue cracking initiated at fusion lines. In axial tests cracks grew in the circumferential direction, and in torsional tests cracks grew along fusion lines. The test results were compared to empirical models of fatigue life based on two definition of equivalent normal strain range. The results have provided some needed confirmation of the reduction factors for creep strength and fatigue life of modified 9 Cr-1 Mo steel weldments currently under consideration by ASME Code committees. 8 refs., 5 figs.
A major revolution in the chemistry laboratory (2018)

&lt;span&gt;A historical perspective on the origins of Microscale Chemistry in the USA and of the motivation behind it is presented here by one of the fathers of Microscale Chemistry and his colleagues. The success achieved can be understood in the light of the establishment of many programs and centers around the world.&lt;/span&gt;
Diabetic kidney disease in thedb/dbmouse (2003)

Diabetic nephropathy is increasing in incidence and is now the number one cause of end-stage renal disease in the industrialized world. To gain insight into the genetic susceptibility and pathophysiology of diabetic nephropathy, an appropriate mouse model of diabetic nephropathy would be critical. A large number of mouse models of diabetes have been identified and their kidney disease characterized to various degrees. Perhaps the best characterized and most intensively investigated model is the db/db mouse. Because this model appears to exhibit the most consistent and robust increase in albuminuria and mesangial matrix expansion, it has been used as a model of progressive diabetic renal disease. In this review, we present the findings from various studies on the renal pathology of the db/db mouse model of diabetes in the context of human diabetic nephropathy. Furthermore, we discuss shortfalls of assessing functional renal disease in mouse models of diabetic kidney disease.
Dynamic camera calibration of roadside traffic management cameras for vehicle speed estimation (2003)

In this paper, we present a new three-stage algorithm to calibrate roadside traffic management cameras and track vehicles to create a traffic speed sensor. The algorithm first estimates the camera position relative to the roadway using the motion and edges of the vehicles. Given the camera position, the algorithm then calibrates the camera by estimating the lane boundaries and the vanishing point of the lines along the roadway. The algorithm transforms the image coordinates from the vehicle tracker into real-world coordinates using our simplified camera model. We present results that demonstrate the ability of our algorithm to produce good estimates of the mean vehicle speed in a lane of traffic.
Reed Solomon Codes for Molecular Communication With a Full Absorption Receiver (2017)

Molecular communication (MC) has recently emerged as a novel paradigm for nano-scale communication utilizing molecules as information carriers. In diffusion-based molecular communication, the system performance is constrained by the inter-symbol-interference caused by the crossover of information carrying molecules in consecutive bits. To cope with this, we propose the Reed-Solomon (RS) codes as an error recovery tool, to improve the transmission reliability in diffusion-based MC systems. To quantify the performance improvement due to RS codes, we derive the analytical expression for the approximate bit error probability (BEP) of the diffusion-based MC system with the full absorption receiver. We further develop the particle-based simulation framework to simulate the proposed system with RS code to verify the accuracy of our derived analytical results. Our results show that, as the number of molecules per bit increases, the BEP of the system with RS codes exhibits a substantial improvement than that of non-coded systems. Furthermore, the BEP of the proposed system with RS codes can be greatly improved by increasing the minimum distance of the codeword.
Rapid epidemiologic analysis of cytomegalovirus by using polymerase chain reaction amplification of the L-S junction region (1992)

A technique based on polymerase chain reaction (PCR) amplification was developed to facilitate the study of the epidemiology of cytomegalovirus (CMV). Consensus oligonucleotide primers from repetitive DNA sequences were designed to amplify interspersed repetitive sequences in an area of heterogeneity within the L-S junction region of the CMV genome, and PCR products were detected by gel electrophoresis. Purified CMV DNAs from 25 CMV isolates, 13 from members of five families in which person-to-person transmission was documented, 9 random clinical isolates of CMV, and 3 laboratory reference strains of CMV (Towne, Davis, and AD169), were analyzed. The gel electrophoretic patterns of DNA bands, or PCR profiles, produced by amplification with the L-S primers were unique for epidemiologically unrelated strains and laboratory reference strains, yet similar patterns were observed for epidemiologically related strains isolated from members of the same family. This method of rapid fingerprinting of CMV DNA within the hypervariable L-S junction region by PCR to produce strain-specific, variably sized PCR products should simplify the molecular epidemiologic analysis of CMV.
Nonocomposites from Coated Nanoparticles (1999)

Microwave plasma synthesis can be used to coat individual ceramic nanoparticles with both ceramic and polymer coatings, as reported here. This enables the production of technical parts with properties characteristic of the isolated particles, such as superparamagnetism. The Figure shows the very narrow size distribution of poly(methacrylic acid)-coated maghemite particles produced with this method.
Recommendations regarding technical standards for follow-on biologics: comparability, similarity, interchangeability (2009)

In the authors' view, analytical methodology in its current state cannot alone provide full assurance that the FOB is sufficiently similar to the innovator product. Moreover, the FOB manufacturer will not have access to the extensive knowledge accumulated by the innovator manufacturer from early development through marketing. Thus, extensive clinical evaluation will likely be necessary to provide assurance that the FOB is safe and efficacious. If such testing demonstrates the FOB is safe and efficacious per existing regulatory standards, the product should receive marketing approval as a 'similar' product. Since 'similarity' is a fundamentally different determination than establishing interchangeability between the two products, an interchangeability determination must be based on additional testing and market experience to ensure patient safety. Post-marketing surveillance of the FOB should be conducted to ensure that the approved molecule has similar clinical safety and efficacy as the innovator product, prior to any consideration of interchangeability.
Tracing functionally identified neurones in a multisynaptic pathway in the hamster and rat using herpes simplex virus expressing green fluorescent protein (2001)

Using a genetically modified herpes simplex virus encoding green fluorescent protein we sought to establish if this viral modification could be used in transneuronal tracing studies of the sympathetic nervous system. The herpes simplex virus encoding green fluorescent protein was injected into the adrenal medulla of three hamsters and six rats. After a suitable survival period, neurones in the sympathetic intermediolateral cell column of the thoracolumbar spinal cord, rostral ventral medulla and paraventricular nucleus of the hypothalamus were clearly identified by the presence of a green fluorescence in the cytoplasm of the neurones of both species. Thus, herpes simplex virus encoding green fluorescent protein labelled chains of sympathetic neurones in the hamster and rat and therefore has the potential to be used in transneuronal tracing studies of autonomic pathways in these species.