gebhart/patrepeval huggingface dataset:
V2 hotspot optimized MVA vaccine expressing stabilized HIV-1 Clade C envelope Gp140 delays acquisition of heterologous Clade C Tier 2 challenges in Mamu-A*01 negative Rhesus Macaques (2022)

Stabilized HIV envelope (Env) trimeric protein immunogens have been shown to induce strong autologous neutralizing antibody response. However, there is limited data on the immunogenicity and efficacy of stabilized Env expressed by a viral vector-based immunogen. Here, we compared the immunogenicity and efficacy of two modified vaccinia Ankara (MVA) vaccines based on variable loop 2 hotspot (V2 HS) optimized C.1086 envelope (Env) sequences, one expressing the membrane anchored gp150 (MVA-150) and the other expressing soluble uncleaved pre-fusion optimized (UFO) gp140 trimer (MVA-UFO) in a DNA prime/MVA boost approach against heterologous tier 2 SHIV1157ipd3N4 intrarectal challenges in rhesus macaques (RMs). Both MVA vaccines also expressed SIVmac239 Gag and form virus-like particles. The DNA vaccine expressed SIVmac239 Gag, C.1086 gp160 Env and rhesus CD40L as a built-in adjuvant. Additionally, all immunizations were administered intradermally (ID) to reduce induction of vaccine-specific IFNγ+ CD4 T cell responses. Our results showed that both MVA-150 and MVA-UFO vaccines induce comparable Env specific IgG responses in serum and rectal secretions. The vaccine-induced serum antibody showed ADCC and ADCVI activities against the challenge virus. Comparison with a previous study that used similar immunogens <i>via</i> intramuscular route (IM) showed that ID immunizations induced markedly lower SHIV specific CD4 and CD8 T cell responses compared to IM immunizations. Following challenge, MVA-UFO vaccinated animals showed a significant delay in acquisition of SHIV1157ipd3N4 infection but only in Mamu-A*01 negative macaques with an estimated vaccine efficacy of 64% per exposure. The MVA-150 group also showed a trend (p=0.1) for delay in acquisition of SHIV infection with an estimated vaccine efficacy of 57%. The vaccine-induced IFNγ secreting CD8 T cell responses showed a direct association and CD4 T cells showed an inverse association with delay in acquisition of SHIV infection. These results demonstrated that both MVA-150 and MVA-UFO immunogens induce comparable humoral and cellular immunity and the latter provides marginally better protection against heterologous tier 2 SHIV infection. They also demonstrate that DNA/MVA vaccinations delivered by ID route induce better antibody and lower CD4 and CD8 T cell responses compared to IM.
A possible genetic-algorithm based method for optimizing a class of ANN transfer functions (2003)

This paper proposes a hybrid of two methods to determine the weight-constants in a class of artificial neuron networks, ANNs. The class of ANNs we are interested in are characterized by feed-forward processing elements. One of the methods is the genetic algorithm, GA; the other is "training through" back-propagation of the error, BPE. We expect our hybrid scheme to be faster than using BPE alone.
El derecho humano al medio ambiente sano para el desarrollo y bienestar (2014)

Documento en el que se exponen las cuestiones mas relevantes sobre el medio ambiente y su proteccion legal en Mexico.
Substance P in the serum of patients with rheumatoid arthritis. (1997)

Serum substance P was assayed in rheumatoid arthritis patients and healthy controls to evaluate whether neurogenic inflammation with substance P release is significant in rheumatoid arthritis. A very sensitive competitive immunoenzymetric assay was used. Mean serum substance P level was significantly higher in rheumatoid arthritis patients than in controls and was not correlated with disease duration, morning stiffness duration, Thompson's articular index, Larsen's radiographic score, or the following laboratory indices of inflammation: erythrocyte sedimentation rate, C-reactive protein, and alpha 1-acid glycoprotein. Neurogenic inflammation with substance P release may contribute significantly to the pathogenesis of rheumatoid arthritis. The absence of correlations between serum substance P and clinical or laboratory indices of inflammation may reflect complex interactions between neurogenic inflammation and other pathogenic mechanisms, which may influence clinical features and laboratory tests in rheumatoid arthritis patients.
Lessons from Kitty Hawk: From feasibility to routine clinical use for the field of proteomic pattern diagnostics (2004)

Proteomic pattern diagnostics is a rapidly evolving field of science. Despite the increasingly large number of laboratories reporting exciting success with this concept, recent speculation concerning reproducibility and the nature and identities of the information content of the pattern constituents have served to defocus and polarize the community. These controversies will be rendered obsolete as the field accelerates into a new realm of clinical diagnostics. This new era will see the currently dry biomarker pipeline flooded with new candidate molecules, and the mass spectrometer will continue its maturation into a dominant clinical platform. We reflect on the important lessons gleaned from the Wright brothers' attempts at controlled heavier-than-air flight as a model for perseverance and a view to the very near future for proteomic pattern diagnostics.
SAMstrt: statistical test for differential expression in single-cell transcriptome with spike-in normalization (2013)

shintaro.katayama@ki.se
ChemInform Abstract: Pyridine‐Substituted Hydroxythiophenes. Part 1. Preparation of o‐(2‐, 3‐ and 4‐Pyridyl)‐3‐hydroxythiophenes. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
APPLICATION OF PIXE TO DETERMINATION OF ELEMENTS IN THE PLACENTA, MATERNAL BLOOD AND CORD BLOOD (1997)

PIXE technique was applied to the measurement of elements in the placenta, maternal blood and cord blood of human. The elements determined in these samples include Cl , K , Ca , Fe , Cu and Zn . The values obtained by PIXE were compared with those by ICP-AES to test the accuracy of the method. In placental samples, the mean values of K , Fe , Cu and Zn concentrations obtained by the two methods agree, while Ca concentration is lower in PIXE analysis. The values from PIXE, however, show larger variations resulting from inhomogeneity of the placental samples composed of various tissues different in histological functions. In the analysis of blood samples, the results of the two methods agree for Cl , K , Ca , Fe , Cu and Zn , although the standard deviations tend to be larger in PIXE. These results indicate that PIXE is a useful method for the determination of elements in placental and blood samples, although the preparation to homogenize sample is necessary to obtain accurate results.
Influence of Genotype and CO2 on Discoloration, Phenolic Content, Peroxidase, and Phenolase Activities in Snap Beans1 (1977)

Abstract The role of phenolic content and phenolase activity in the postharvest discoloration of broken snap bean pods ( Phaseolus vulgaris L.) was studied by measuring their levels in broken pods which discolored in 24 hours and in broken pods in which discoloration was inhibited by CO 2 . Elevated CO 2 atmospheres (30%) inhibited the increase in phenolics content but did not affect phenolase activity. In addition, phenolic content, peroxidase, and phenolase activities were determined in 2 genotypes which discolor slightly (‘Blue Crop’ and ‘NCX8005’) and two which discolor severely (‘Provider’ and ‘GP72-122’). Again, discoloration was associated with increased levels of phenolic substances after injury regardless of phenolase and peroxidase activities. Systems which inhibit production of phenolics in response to injury reduce the problem of discoloration.
Mechanically Tunable Thin Films of Photosensitive Artificial Proteins:  Preparation and Characterization by Nanoindentation (2008)

Thin films of controlled elastic modulus were made by photo-cross-linking artificial extracellular matrix (aECM) proteins containing the photosensitive amino acid p-azidophenylalanine (pN3Phe). The elastic moduli of the films were calculated from nanoindentation data collected by atomic force microscopy (AFM) using a thin-film Hertz model. The modulus was shown to be tunable in the range 0.3−1.0 MPa either by controlling the irradiation time or by varying the level of pN3Phe in the protein. Tensile measurements on bulk films of the same proteins and finite-element simulation of the indentation process agreed with the thin-film modulus measurements from AFM. Substrates characterized by spatial variation in elastic modulus were created by local control of the irradiation time.
The politics of authenticity in postmodern rock culture: The case of Negativland andthe letter ‘U’ and the numeral ‘2‘ (1998)

Based on recent concerns with the notion of authenticity and effective politics in contemporary popular culture and scholarship on culture, this essay uses a case study of the legal and popular controversy surrounding the Negativland recording, “The Letter ‘U’ and the Number ‘2’.” The analysis points to the organic development of alternative logics in the changing landscape of popular culture. Moreover, we point to the relationship between the “pastiche” style of the Negativland recording as a metaphor for authenticity and justice in postmodern rock culture.
Achieving Cultural Safety in Australian Indigenous Maternity Care (2015)

Greater attention needs to be paid to BPPV patients. Vulnerable female patients may have high levels of psychiatric symptomatology that may benefit from assessment and intervention.
MACROMOLECULAR CHARGE AND CELLULAR SURFACE CHARGE IN ADHESION, INGESTION, AND BLOOD VESSEL LEAKAGE (1983)

To get reliable information about the role of charged groups of tissue components on the integrity of blood circulation, the staining and perfusion experiments of rat organs with cationic (cacodylate iron colloid) and anionic iron colloid (chondroitin iron colloid) particles were carried out. These cationic and anionic iron colloid particles are stable in a wide range of pH's. Dissociation curves of several tissue components at varied pH levels were observed for the analysis of the results obtained by histochemical observations and perfusion experiments. The results indicated that all the intra- and extracellular spaces of living tissues are surrounded by the ionized anionic groups. No ionized cationic groups were found except on the macrophage surface. The cationic iron colloid particles perfused into organs through vessels adhered to the endothelial cell surface inducing the swelling of the cells. The cationic particles invaded into surrounding tissues passing through the damaged endothelial layer, but plasma protein leakage did not occur being prevented by the newly formed fibrin-fibrinogen lining. The anionic iron colloid particles introduced into vessels did not adhere to blood vessel walls but were taken by reticuloendothelial cells or macrophages. The importance of the ionized anionic groups of proteins on the endothelial cell surface and surrounding tissues for the integrity of blood vessel function was stressed.
Adsorption Studies on Metals. I. Physical and Chemical Adsorption of Gases on Molybdenum (1953)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAdsorption Studies on Metals. I. Physical and Chemical Adsorption of Gases on MolybdenumF. H. Healey, J. J. Chessick, and A. C. ZettlemoyerCite this: J. Phys. Chem. 1953, 57, 2, 178–182Publication Date (Print):February 1, 1953Publication History Published online1 May 2002Published inissue 1 February 1953https://pubs.acs.org/doi/10.1021/j150503a010https://doi.org/10.1021/j150503a010research-articleACS PublicationsRequest reuse permissionsArticle Views83Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Antimicrobial susceptibility testing of Haemophilus influenzae Improvement of accuracy of the disc diffusion test (1990)

A national quality control study was performed in 1986 to investigate the standard of performance of the disc diffusion antimicrobial susceptibility testing of Haemophilus influenzae in Sweden. The accuracy of susceptibility interpretations was unacceptably low. A new standardized method for susceptibility testing of H. influenzae was then worked out, and a new method of setting interpretive zone breakpoints was introduced. The susceptibility category of the main population of clinical isolates was determined according to the MIC50 of the strains. The zone histograms of clinical isolates from five reference laboratories were used for the calculation of interpretive breakpoints. For instance, for susceptible strains the mean of the combined zone values from these laboratories +/- 2 S.D. covered the zone range of the susceptible group, and one more S.D. below covered the intermediate/indeterminate group. The new zone breakpoints would place the main population of clinical isolates in the correct susceptibility group, and even make it possible to detect strains with different degrees of reduced susceptibility in the routine test. In a follow-up quality control study in 1988 the interpretive errors for clinical isolates were eliminated for all antibiotics except doxycycline in some laboratories. Laboratory-related zone breakpoints for doxycycline calculated by the single strain regression analysis method led to correct susceptibility interpretations also in these cases.
Participation of MCP-induced protein 1 in lipopolysaccharide preconditioning-induced ischemic stroke tolerance by regulating the expression of proinflammatory cytokines (2011)

Our data indicate that absence of MCPIP1 exacerbates ischemic brain damage by upregulation of proinflammatory cytokines and that MCPIP1 participates in LPS-induced ischemic stroke tolerance.
Analysis of c-erbB-2 expression in breast carcinomas with clinical follow-up. (1989)

Various monoclonal antibodies reactive with protooncogene products or tumor-associated antigens have been utilized to investigate breast carcinoma biology or antigen expression with potential prognostic relevance. Murine monoclonal antibody TA1, generated by immunization of BALB/c mice with whole c-erbB-2 (neu) transformed NIH/3T3 cells, recognizes the extracellular domain of the c-erbB-2 protein and binds a Mr 185,000 protein by immunoprecipitation. Using avidin-biotin-peroxidase techniques and monoclonal antibody TA1, 313 archival primary adenocarcinomas of the breast were evaluated for c-erbB-2 overexpression; 290 of these were used for multiparametric statistical analysis. Historical, clinical (age, laterality), histological (nuclear grade, tumor size, lymph node status, lymphatic or blood invasion), and hormone receptor data as well as clinical outcome (minimal follow-up, 6 years; median follow-up, 8.5 years) were compared to TA1 staining. For these 290 patients Cox regression multivariate analysis showed the strongest correlation between lymph node status or estrogen receptor status and overall survival (P = 0.0001 and 0.049, respectively). TA1 staining did not significantly correlate with survival (P = 0.395). However, univariate analysis of certain patient subpopulations showed a significant correlation if the examined tumors were subdivided into negative or focally reactive and those with greater than or equal to 40% cellular reactivity. For T3, T4 patients, strong TA1 immunoreactivity correlated with a shortened disease-free survival (log rank P = 0.0018; Wilcoxon p = 0.0078) and overall survival (log rank P = 0.0002; Wilcoxon P = 0.0013). For these patients the overall survival at 6 years was markedly different between the strongly reactive tumors (0%) and the negative to weakly reactive tumors (55%). In lymph node-positive patients a trend between high TA1 reactivity and a worse overall survival was also noted (log rank P = 0.128; Wilcoxon P = 0.054), with a 6-year survival of 42% in the strongly reactive tumors (n = 16) and 65% in the negative to weakly reactive carcinomas (n = 105). No correlation between TA1 immunoreactivity and other historical, clinical, and histological features were noted. c-erbB-2 overexpression as measured by immunohistochemical techniques, therefore, may have clinical significance in certain patient subpopulations.
Location of Cholic Acid Sequestered by Core−Shell Nanoparticles Using REDOR NMR (2001)

Solid-state 13C{19F} rotational-echo double-resonance (REDOR) NMR was used to locate [24-13C]cholic acid that had been absorbed by nanoparticles with a core−shell morphology. The nanoparticles were composed of poly(4-fluorostyrene)-block-poly(acrylic acid) copolymers in which 15% of the poly(acrylic acid) shell functionality had been cross-linked with 2,2‘-(ethylenedioxy)bis(ethylamine). The REDOR data were consistent with no cholic acid in the core, approximately 28% located at or near the core−shell interface, and the remaining 72% of the cholic acid farther than 25 Å from the interface.
Rayleigh segmentation of the endocardium in ultrasound images (2008)

In this paper we present the Coupled Active Contours (CAC) model, which is applied to segmentation of the endocardium in ultrasonic images assuming Rayleigh distributed intensities. Comparative experiments, both real and synthetic, with a standard prior model are presented. In the CAC model the prior acts, by affine transformation, on the same image information as the active contour, in addition to the traditional interaction between prior and active contour. By this higher convergence rate and robustness, w.r.t artifacts and poor initialization, is achieved.
Antiserum to Tumor Necrosis Factor and Failure to Prevent Murine Colitis (1995)

Cytokines regulate many aspects of disease and have been implicated as mediators of the inflammatory reactions in patients with both ulcerative (UC) and Crohn's colitis. We examined the local and systemic appearance of tumor necrosis factor (TNF) and interleukin 6 (IL-6) in an experimental animal model of inflammatory bowel disease. Colitis was induced in CBA/J mice by adding dextran sulfate sodium (DSS), 5% (wt/vol), to their water. DSS-induced colitis is a reproducible animal model for evaluating the role of cytokines in the pathology of colitis. Animals were weighed daily, and stools were checked for the presence of blood. Groups of mice were killed daily, blood samples were taken for measurement of plasma cytokine levels, and colonic samples were taken for histology and measurement of TNF and IL-6 bioactivity. Mice fed DSS developed colitis with bloody diarrhea, weight loss, and colonic inflammation by days 5-9. Histologic examination of the colons showed focal crypt destruction and ulceration. In mice with DSS-induced colitis no TNF was detectable in colonic tissue extracts or in plasma. In contrast, plasma IL-6 was detectable from days 4 to 9 and was detectable in colonic tissue in only a few (two of four) terminally ill animals on day 9. Animals were injected with a neutralizing, polyclonal anti-TNF antiserum that maintained high in vivo neutralizing titers for > or = 48 h. This anti-TNF antiserum failed to block or modify the severity of colitis induced by DSS. Failure to detect local or systemic TNF and failure to prevent colonic inflammation with anti-TNF antiserum showed that TNF is not an inflammatory mediator in DSS-induced murine colitis.
Apolipoprotein A-I Modulates Processes Associated with Diet-Induced Nonalcoholic Fatty Liver Disease in Mice (2012)

Apolipoprotein A-I (apoA-I) is the main protein of high-density lipoprotein (HDL). We investigated the involvement of apoA-I in diet-induced accumulation of triglycerides in hepatocytes and its potential role in the treatment of nonalcoholic fatty liver disease (NAFLD). ApoA-I-deficient (apoA-I(-/-)) mice showed increased diet-induced hepatic triglyceride deposition and disturbed hepatic histology while they exhibited reduced glucose tolerance and insulin sensitivity. Quantification of FASN (fatty acid synthase) [corrected], DGAT-1 (diacylglycerol O-acyltransferase 1), and PPARγ (peroxisome proliferator-activated receptor γ) mRNA expression suggested that the increased hepatic triglyceride content of the apoA-I(-/-) mice was not due to de novo synthesis of triglycerides. Similarly, metabolic profiling did not reveal differences in the energy expenditure between the two mouse groups. However, apoA-I(-/-) mice exhibited enhanced intestinal absorption of dietary triglycerides (3.6 ± 0.5 mg/dL/min for apoA-I(-/-) versus 2.0 ± 0.7 mg/dL/min for C57BL/6 mice, P < 0.05), accelerated clearance of postprandial triglycerides and a reduced rate of hepatic very low density lipoprotein (VLDL) triglyceride secretion (9.8 ± 1.1 mg/dL/min for apoA-I(-/-) versus 12.5 ± 1.3 mg/dL/min for C57BL/6 mice, P < 0.05). In agreement with these findings, adenovirus-mediated gene transfer of apoA-I(Milano) in apoA-I(-/-) mice fed a Western-type diet for 12 wks resulted in a significant reduction in hepatic triglyceride content and an improvement of hepatic histology and architecture. Our data extend the current knowledge on the functions of apoA-I, indicating that in addition to its well-established properties in atheroprotection, it is also an important modulator of processes associated with diet-induced hepatic lipid deposition and NAFLD development in mice. Our findings raise the interesting possibility that expression of therapeutic forms of apoA-I by gene therapy approaches may have a beneficial effect on NAFLD.