gebhart/patrepeval huggingface dataset:
Monoclonal antibodies reveal cell-type-specific antigens in the sexually dimorphic olfactory system of Manduca sexta. II. Expression of antigens during postembryonic development (1988)

Two classes of monoclonal antibodies specific to the olfactory system of Manduca sexta have been isolated: the olfactory-specific antibody (OSA), which specifically recognizes many or all olfactory receptor cells (ORCs) in both males and females, and the male olfactory-specific antibody (MOSA), which stains male-specific receptor cells (principally or exclusively sex-pheromone receptors present only in antennae of males; Hishinuma et al., 1988). In the investigation reported here, we examined the expression of the antigens during postembryonic development in order to correlate the presence of particular antigens with the status of differentiation of the ORCs or with their acquisition of particular functions. As assessed immunocytochemically, the OSA recognizes certain epithelial cells in the antennal imaginal disk of the fifth-instar larva. Later, during the first 70 hr of adult development, when differentiative cell divisions are occurring in the antennal epithelium to generate ORCs and the other cells that make up olfactory sensilla, no cells are stained. Immediately after this period of mitoses, the OSA immunoreactivity reappears exclusively in the ORCs, which begin to elaborate axons as an early event in their differentiation. On immunoblots, the OSA recognizes specific sets of molecules (distinguished on the basis of their apparent molecular weights): 53,000 and 59,000 Da antigens in the disk epithelial cells in the last-instar larva; 53,000, 59,000, and 66,000 Da antigens in the ORCs from 15 to 60% of metamorphic adult development; and 42,000, 59,000, and 66,000 Da antigens in the ORCs from 60 to 100% of adult development. The MOSA also recognizes a subset of the epithelial cells in the antennal disks in male and female larvae.(ABSTRACT TRUNCATED AT 250 WORDS)
Antigen analysis of several pathogenic strains of Trichomonas vaginalis (1983)

Analysis of several human strains of Trichomonas vaginalis and one bovine strain of Tritrichomonas foetus was accomplished with standard sodium dodecyl sulfate-gel electrophoresis and fluorography technology. Highly motile, live trichomonads were radiolabeled, and total trichloroacetic acid-precipitated proteins were electrophoresed. Complex protein profiles of the various human strains of T. vaginalis were obtained with proteins ranging in molecular weight from 20,000 to greater than 200,000. The parasite biosynthesis of the Coomassie brilliant blue-stained protein bands was demonstrated by efficient radiolabeling of trichomonads with [35S]methionine or a 3H-amino acid digest before electrophoresis and fluorography. Immunogenic trichomonal proteins were then identified by a radioimmunoprecipitation method. A detergent extract of [35S] methionine-labeled T. vaginalis proteins was mixed with serum from an immunized rabbit or pooled sera from subcutaneously infected mice and soluble antibody-antigen complexes isolated by adsorption to protein A-bearing Staphylococcus aureus. The radiolabeled protein antigens were then identified by gel electrophoresis and fluorography. Immunized rabbit serum and pooled sera from challenged mice contained high-titered antibody which reacted with numerous high- and low-molecular-weight proteins. Individual subcutaneously infected mice were found to possess identical antibody responses to these immunogenic trichomonal proteins. A high degree of serological cross-reactivity among the various trichomonads was demonstrated. No differences in the composition of immunogenic proteins were observed among cultures grown in vitro for various lengths of time under the experimental conditions employed. Finally, electrophoretic analysis of cloned colonies of T. vaginalis organisms revealed no differences in their protein composition. The biological relevance of these observations is discussed.
41st Annual David W. Smith workshop on malformations and morphogenesis: Abstracts of the 2020 annual meeting (2021)

Abstract The 41st Annual David W. Smith Workshop on Malformation and Morphogenesis was scheduled to take place in Skamania, Washington, on September 11–16, 2020. Due to the COVID‐19 pandemic and the associated recommendations to avoid travel and congregation in large groups, this meeting took place differently from its original plan. Rather than bringing trainees, clinicians and researchers with an interest in congenital malformations and their underlying morphogenesis together for several days in a workshop with submitted presentations and research lectures, this meeting took place virtually. A 1 day online meeting was organized in order to allow trainees to present their work. This Conference Report includes the highest scoring abstracts submitted by trainees and presented at the 2020 virtual David W. Smith Workshop.
Carrier Acquisition for Coherent Demodulation of Pulse-Amplitude Modulation (1970)

Phase recovery in vestigial sideband (VSB) data transmission is discussed. Examining a particular aspect of this broad area, attention is focused on the time required to recover carrier phase in order to achieve a satisfactory error rate. We derive the acquisition time for simple models. Our results are exhibited graphically, relating pertinent system parameters. These results indicate that while in double sideband transmission the acquisition time required to achieve a satisfactory error rate is of the order of a few symbol intervals, in VSB transmission this time grows to tens and hundreds of symbol intervals.
A Population Genomics Analysis of the Native Irish Galway Sheep Breed (2019)

The Galway sheep population is the only native Irish sheep breed and this livestock genetic resource is currently categorised as 'at-risk'. In the present study, comparative population genomics analyses of Galway sheep and other sheep populations of European origin were used to investigate the microevolution and recent genetic history of the breed. These analyses support the hypothesis that British Leicester sheep were used in the formation of the Galway. When compared to conventional and endangered breeds, the Galway breed was intermediate in effective population size, genomic inbreeding and runs of homozygosity. This indicates that, although the Galway breed is declining, it is still relatively genetically diverse and that conservation and management plans informed by genomic information may aid its recovery. The Galway breed also exhibited distinct genomic signatures of artificial or natural selection when compared to other breeds, which highlighted candidate genes that may be involved in production and health traits.
What are the future trends in high-performance inter.connects for parallel computers? [Panel 1] (2004)

Most interconnection networks provide some form of intelligence in the interface. Do you expect this to become a central feature in the future? Will it be possible to implement network operating systems in the interface? Do you expect that optical networks will become widespread? What are the trends in latency and bandwidth? Networks as Quadrics Elan4 and Cray XDl's already deliver 1.5 microseconds at MPI level. Is bandwidth technologically free? Will the I/O interface be the bottleneck? Will native support for collective communication be a central feature of a high performance network? BlueGenelL has shown that a thermally-aware supercomputer can be packaged in a small space, with a chip that integrates processors and interface. Will future interfaces be integrated with the processors in the same chip? How can a high-performance help to achieve fault-tolerance in a large-scale machine?
Development Trends of Direct Methanol Fuel Cells (2002)

DMFC is one of the fuel cells that generate electricity using aqueous methanol solution as a fuel and oxygen or air as an oxidizing agentBy operation of DMFCmethanol is oddized by oxygen to produce carbon dioxide Imd waterA single cell of DMFC is composed of an MEA that is held between separatorsMEA is manufactured by laminating anode an(1cathode on both sides of polymer electrolyte membrane which is made of sulfonized perfluorocarbon polymer or hydrocarbon polymerRecently limiting current has increased up to600mAcm20wing to the advance in MEA technologyDMFC can be downsized because they use liquid fuel directlywhich is more preferable for applications such as portable and mobile useHoweverthere are many technological problems that should be overcome to realize DMFC system to be used for those applicationsEspeciallya breakthrough in materials research should be necessary in order to decrease methanol crossover and to improve catalytic activity DMFCMethano1MEAPolymer ElectrolyteCrossoverCatalyst l lntrOdUCtiOn
Toward using confidence intervals to compare correlations. (2007)

Confidence intervals are widely accepted as a preferred way to present study results. They encompass significance tests and provide an estimate of the magnitude of the effect. However, comparisons of correlations still rely heavily on significance testing. The persistence of this practice is caused primarily by the lack of simple yet accurate procedures that can maintain coverage at the nominal level in a nonlopsided manner. The purpose of this article is to present a general approach to constructing approximate confidence intervals for differences between (a) 2 independent correlations, (b) 2 overlapping correlations, (c) 2 nonoverlapping correlations, and (d) 2 independent R2s. The distinctive feature of this approach is its acknowledgment of the asymmetry of sampling distributions for single correlations. This approach requires only the availability of confidence limits for the separate correlations and, for correlated correlations, a method for taking into account the dependency between correlations. These closed-form procedures are shown by simulation studies to provide very satisfactory results in small to moderate sample sizes. The proposed approach is illustrated with worked examples.
Drumming with dopamine neurons: Resonance and synchronization in the Ventral Tegmental Area (2018)

The ventral tegmental area (VTA) is a dopaminergic nucleus in the midbrain with the propensity to exhibit spontaneous intrinsic rhythmic activity in the 1-5 Hz frequency range (ex vivo). Here, we combine in-vitro simultaneous action potential recording from a 60 channel multi-electro-array with experimental manipulations to investigate the local VTA network. First, we increased the neuronal excitability with two compounds (extracellular potassium and glutamate). The functional connectivity between dopamine neurons was measured with the Paired-Phase-Consistency. Manipulating excitability had distinct and specific consequences for functional connectivity in the VTA network separate from the e_ects on neuronal firing rates. Thus the functional connectivity within the VTA is an emergent phenomena. Secondly, we observed that the dopamine sensitivity (EC50) of the neurons (the relation between dopamine concentration and firing rate) was variable within the recorded population. Dopamine sensitivity was found to determine the role of a neuron in the local VTA network. Highly sensitive neurons became followers, whereas less sensitive dopamine neurons played a more leading role (quantified by Granger causality). Dopamine sensitivity thus tunes the network interactions between VTA dopamine neurons. Thirdly, the dopamine neuron population activity was manipulated with precise laser pulse stimulation (optogenetics). The filter characteristics of the VTA dopamine neurons, measured with this technique, allow the VTA to encode timing information in its input important for reward prediction. Our study reveals emergent network phenomena in the VTA relevant for pharmacology targeting dopamine and the function of the local VTA network in relation to reward prediction, memory and learning.
Text (2006)

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Method Development and Validation of RP-HPLC Method for Determination of New Antipsychotic Agent Asenapine Maleate in Bulk and Pharmaceutical Formulation (2014)

A novel isocratic reverse phase liquid chromatography method for determination of Asenapine Maleate was developed and validated after optimization of various chromatographic conditions. A Hiber  C18, 5 μm column having 250×4.6 mm i.d., with mobile phase containing 0.05 M potassium dihydrogen phosphate : acetonitrile (60:40, v/v, pH  2.7 adjusted with 1% o-phosphoric acid) was used. The flow rate was 1.0 mL min -1 and effluents were monitored at 270 nm. The retention time of asenapine was 4.2min. The linearity for Asenapine maleate was in the range of 0-150 μg mL -1 with coefficient of correlation 0.999 . The proposed method was validated with respect to linearity, accuracy, precision and robustness.
Active feedback stabilization of the resistive wall mode on the DIII-D device (2001)

A proof of principle magnetic feedback stabilization experiment has been carried out to suppress the resistive wall mode (RWM), a branch of the ideal magnetohydrodynamic (MHD) kink mode under the influence of a stabilizing resistive wall, on the DIII-D tokamak device [Plasma Phys. Controlled Fusion Research (International Atomic Energy Agency, Vienna, 1986), p. 159; Phys. Plasmas 1, 1415 (1994)]. The RWM was successfully suppressed and the high beta duration above the no-wall limit was extended to more than 50 times the resistive wall flux diffusion time. It was observed that the mode structure was well preserved during the time of the feedback application. Several lumped parameter formulations were used to study the feedback process. The observed feedback characteristics are in good qualitative agreement with the analysis. These results provide encouragement to future efforts towards optimizing the RWM feedback methodology in parallel to what has been successfully developed for the n=0 vertical positional control. Newly developed MHD codes have been extremely useful in guiding the experiments and in providing possible paths for the next step.
Impaired catalytic function of activated protein C: a new in vitro manifestation of lupus anticoagulant (1989)

Lupus anticoagulant (LA), an antibody against anionic phospholipid with anticoagulant laboratory manifestations, is paradoxically associated with a high incidence of thrombosis. In the present study we analyzed the phospholipid- and platelet-dependent degradation of factor Va following clotting in plasma from 15 consecutive patients with LA to provide evidence for a distinct procoagulant effect of the antibody. After clotting with 25 micrograms phospholipid/mL, all samples containing LA showed markedly decreased rates of factor Va degradation (k = 0.01 to 0.14 min-1 v 0.27 to 0.35 min-1 in controls). Also with higher phospholipid concentrations (up to 100 micrograms/mL), as well as in the presence of platelets (5 to 33 x 10(7)/mL), significantly less of the procoagulant activity disappeared per unit of time in samples with LA than in controls. Plasma with LA was to a variable extent capable of decreasing or abolishing factor Va inhibition in normal plasma. Most importantly, exogenous activated protein C failed to correct the ineffective factor Va destruction despite adequate protein S levels. These data suggest that LA prevents the formation of the complex essential for rapid proteolysis of factor Va both on phospholipid and on the platelet membrane, thereby compromising the catalytic function of activated protein C. Our findings offer a new opportunity for a more comprehensive evaluation of patients with antiphospholipid antibody in defining the pathogenesis of thrombosis in this clinical condition.
Fatigue properties of hot isostatically pressed Ti-6Al-4V powders (1983)

This work is part of an extensive effort to select the best titanium alloy powder and processing method for the production of high quality aerospace components. Fatigue behavior was identified as the most critical property and was the focus of this investigation. Two powder production methods, Rotating Electrode Process (REP) and the electron beam method in which the powder is produced under vacuum (PSV), were studied. In addition to straight Hot Isostatic Pressing (HIP) of the powders, processing sequences included as variables were strain-energizing of the powder by passing it through a rolling mill, and various particle size fractions. The fatigue behavior of the compacted materials is discussed in terms of the basic microstructures and the contaminants present. Consideration is given to the influence of powder size on the fatigue performance. It is shown that clean powder can produce both tensile and fatigue strength levels equivalent to those observed in the same alloy produced by ingot metallurgy (IM). It is anticipated that the reported improvement in mechanical properties coupled with the inherent net shape advantages of this technology will further promote the use of titanium alloy powder metallurgy (PM) aerospace components.
An Innovative Photonic Integrated Channelizer Design for Hyperspectral Microwave Sounding (2021)

A photonic integrated channelizer is designed for hyperspectral and ultra-high band coverage remote sensing applications. The tunable delay lines maximize the transmission performance and the device demonstrates 4.5 GHz of optical bandwidth and channel separation. OSCIS codes : (250.5300) Photonic Integrated circuits; (280.4991) Passive remote sensing
Antimony-assisted carbonization of Si(111) with solid source molecular beam epitaxy (2013)

The carbonization of an antimony-terminated Si (111) surface in a solid source molecular beam epitaxy system is presented. Reflection high-energy electron diffraction, atomic force microscopy, x-ray photoelectron spectroscopy, and cross-sectional transmission electron microscopy are used to characterize samples grown with and without antimony termination. It is shown that the antimony-terminated surface promotes the formation of thin, smooth and continuous SiC films at a relatively low temperature of 800 °C.
Growth hormone regulation of glomerular AT1angiotensin receptors in adult uninephrectomized male rats (2003)

Sex differences exist in the mechanisms initiating early compensatory renal growth after unilateral nephrectomy (UNX); remnant kidney growth is growth hormone (GH) independent in adult female rats and GH dependent in adult male rats. The present study determined whether sex differences also exist in angiotensin type 1 receptor (AT1R) regulation during early remnant kidney (REM) growth after UNX, and if so, whether GH modulates AT1R expression after UNX in the male rat. Scatchard analysis of radioligand binding in glomeruli demonstrated that 48 h post-UNX, AT1R density (Bmax) was significantly decreased by 20% in female REM compared with control kidneys. In contrast, male REM glomerular Bmax was significantly increased by 28% compared with control kidneys. Furthermore, GH-suppressed male rats displayed attenuated REM growth, which was associated with a 35% decrease in AT1R Bmax. Losartan treatment also decreased REM AT1R Bmax by 55%. The activity of mRNA binding proteins that bind to the 5' leader sequence of the AT1R was regulated by UNX and GH treatment in an inverse manner to AT1R expression. These findings suggest that in rats 1) there are sex differences in the regulation of glomerular AT1R expression after UNX; 2) the increase in AT1R binding sites in the male REM is regulated by GH and mediates early remnant kidney growth; and 3) AT1R 5' leader sequence mRNA binding proteins play a role in UNX and GH regulation of glomerular AT1Rs in both males and females.
Collaborative approach for dynamic adjustment of selection areas in polygonal modelling (2012)

Mutual awareness between users working in collaborative virtual environments is an important factor for efficient collaboration. This article presents a collaborative metaphor dedicated to the dynamic adjustment of selection area in polygonal modelling.
Synthesis and in vivo evaluation of 18F-desbromo-DuP-697 as a PET tracer for cyclooxygenase-2 expression. (2003)

Because of its ability to cross the blood-brain barrier, (18)F-desbromo-DuP-697 appears to be suitable for COX-2 imaging in the brain. Its high nonspecific uptake in the intestines may limit its use for imaging in the abdominal region.
Process and Mechanism of Phase Separation in Polymer Mixtures with a Thermotropic Liquid Crystalline Copolyester as One Component (1996)

The phase separation and the coarsening process of polymer mixtures with a thermotropic liquid crystalline polymer (LCP) as one component are investigated. The LCP used is a main-chain type copolyester X-7G, comprised of p-hydroxybenzoic acid (60 mol %) and ethylene terephthalate (40 mol %) units. The isotropic, transparent, and homogeneous test specimens of the 50/50 mixtures of X-7G and poly(ethylene terephthalate) (PET) were prepared by a rapid solution-casting with an organic solvent. The specimens were heated rapidly to the test temperature (T jump), and an isothermal phase separation process was investigated at real time and in situ under a polarized light microscope. A rapid phase separation was observed, when the temperature was higher than the melting points of PET and X-7G, allowing us to study the late stage spinodal decomposition into anisotropic and isotropic liquid phases. The following sequences of the decomposition mechanisms were found as time elapses in this stage: (i) the self-similar growth of a percolating network of the anisotropic liquid phase rich in X-7G in the isotropic matrix phase rich in PET, (ii) disruption of the percolating network and shrinkage of the disrupted fragments into the anisotropic droplets, and (iii) diffusion and coalescence of the anisotropic droplets. The two important factors, transesterification and the liquid crystal effect which can affect the phase separation, are also discussed in the text.
Prevalence, Factors Associated With, and Prognostic Effects of Preoperative Anemia on Short- and Long-Term Mortality in Patients Undergoing Transcatheter Aortic Valve Implantation (2013)

Preoperative anemia is prevalent in >50% of patients undergoing TAVI. Various baseline factors were related to anemia, which in turn was associated with 1-year mortality. Patients with anemia received more transfusions but mostly for indications unrelated to overt bleeding, whereas transfusion was independently associated with both early and 1-year mortality. These findings indicate that optimization of baseline factors related to preoperative anemia, in addition to more strict criteria of the use of blood products, may improve outcome after TAVI.