105 BLADDER DYSFUNCTION IN MICE WITH EXPERIMENTAL AUTOIMMUNE ENCEPHALITIS IS A MODEL FOR NEUROGENIC OAB IN MULTIPLE SCLEROSIS (2013)

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research (II)1 Apr 2013105 BLADDER DYSFUNCTION IN MICE WITH EXPERIMENTAL AUTOIMMUNE ENCEPHALITIS IS A MODEL FOR NEUROGENIC OAB IN MULTIPLE SCLEROSIS Jan Franken, Mathieu Boudes, Pieter Uvin, Anne-Cathérine Boeve, Thomas Voets, Jerome Hendriks, Niels Hellings, and Dirk De Ridder Jan FrankenJan Franken Leuven, Belgium , Mathieu BoudesMathieu Boudes Leuven, Belgium , Pieter UvinPieter Uvin Leuven, Belgium , Anne-Cathérine BoeveAnne-Cathérine Boeve Leuven, Belgium , Thomas VoetsThomas Voets Leuven, Belgium , Jerome HendriksJerome Hendriks Hasselt, Belgium , Niels HellingsNiels Hellings Hasselt, Belgium , and Dirk De RidderDirk De Ridder Leuven, Belgium View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1484AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Experimental autoimmune encephalitis (EAE) in mice has widely been used as a model for multiple sclerosis (MS). Most MS patients suffer from bladder dysfunction, often refractory to antimuscarinics. It was shown that EAE mice void more frequently and that their bladder walls undergo morphological changes. We investigated whether bladder dysfunction in EAE mice mimics the clinical phenotype in MS patients. METHODS To induce EAE, we injected 12-weeks-old C57/Bl-6 mice with Myelin Oligodendrocyte Glycoprotein (MOG35-55). Controls were injected with Complete Freund's Adjuvant (CFA). We monitored all mice daily for clinical signs of EA and divided them in groups according to severity, with at least 5 animals per group. Scoring went as follows: Score 1, flaccid tail; Score 2, hindlimb weakness; Score 3, hindlimb paralysis; Score 4, paralysis up to diaphragm. First, we assessed muscarinic receptor function and contractile strength in vitro. Therefore, we exposed detrusor strips to increasing concentrations of carbachol (0.1-10μM). Afterwards, maximal contractile strength was measured with a high-potassium solution. Second, in in vivo experiments, bladder capacity (BC) and post-void residue (PVR) were measured. Physiological bladder contractions were assessed with urodynamic investigations in anesthetized mice, by placing a catheter in the dome of the bladder while continuously perfusing it with saline. RESULTS In vitro, maximal contractile strength decreased 30% in response to high potassium, but relative contribution of muscarinic receptors to contractile function was not altered. In vivo, BC increased in EAE mice with score 2 or higher, up to a 2.5 fold increase in score 4. Unlike controls, all animals with EAE score 3 and 4 had a PVR larger than 20μl. In mice with EAE score 4 voiding frequency increased 3.6 fold. In score 3 we observed a 62% and in score 2 a 36% increase, but the latter was not significant. The amount of NVC was significantly higher in score 1, 2 and 3. In score 4 we did not observe NVC, because there was dribbling or voiding in every contraction. Basal pressure increased 2.6 fold in all animals with score 2 or more, whereas maximal pressure was comparable with controls. CONCLUSIONS Bladder dysfunction in EAE mice closely mimics its clinical counterpart in MS. Severity of symptoms on urodynamic investigation always correlated with disease score. Interestingly, in vitro contractile properties were also altered, suggesting that at least part of the urinary symptoms arise from local changes in the bladder. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e42 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jan Franken Leuven, Belgium More articles by this author Mathieu Boudes Leuven, Belgium More articles by this author Pieter Uvin Leuven, Belgium More articles by this author Anne-Cathérine Boeve Leuven, Belgium More articles by this author Thomas Voets Leuven, Belgium More articles by this author Jerome Hendriks Hasselt, Belgium More articles by this author Niels Hellings Hasselt, Belgium More articles by this author Dirk De Ridder Leuven, Belgium More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...