gebhart/patrepeval huggingface dataset:
Single-Molecule Fluorescence Imaging in Living Cells (2013)

The transition of single-molecule fluorescence detection and imaging from in vitro to living cells has greatly enriched our knowledge on the behavior of single biomolecules in their native environments and their roles in cellular processes. Here we review recent advances of single-molecule biophysical approaches to live-cell studies based on fluorescence imaging. We start by discussing the practical considerations in designing single-molecule fluorescence imaging in cells, including the choice of fluorescent probes, labeling methods, instrumentation, and imaging techniques. We then describe representative examples in detail to illustrate the physicochemical parameters that can be obtained by imaging individually labeled biomolecules in cells and what can be learned from such characterizations.
Analysis of a data-based TV–holography system used to measure small vibration amplitudes (1992)

We present a system that measures the full-field amplitude and phase distributions of objects that vibrate with small amplitudes. The system is based on TV–holography combined with sinusoidal phase modulation, discrete vibration-phase shifts, and digital image processing. Different new algorithms, based on a linear approximation of the fringe function, are discussed. Averaging techniques, used to reduce the effects of noise sources and to increase the resolution of the system, are also introduced. For one of the algorithms, tested in combination with the averaging techniques, the amplitude threshold was approximately 1/3000 of the wavelength of the applied laser light. The amplitude resolution was of the same magnitude. The phase accuracy is amplitude dependent and was about 3° for amplitudes greater than 5 nm.
Echinacea (2008)

Echinacea is one of the most popular herbs in modern Europe and United States marketplaces. Unfortunately, most of the Echinacea consumed is misused, based on its misrepresentation as an effective cold and flu remedy. Throughout the later part of the nineteenth century and the first thirty years of the twentieth century, Echinacea was the preferred treatment for infections. The introduction of antibiotics resulted in the eclipse of Echinacea, along with the professional medical doctors, known as the “Eclectics,” who based their treatment on the use of herbs.
Revisiting the Westerlund 2 field with the HESS telescope array (2010)

Aims. Previous observations with the HESS telescope array revealed the existence of extended very-high-energy (VHE; E > 100 GeV) γ-ray emission, HESS J1023–575, coincident with the young stellar cluster Westerlund 2. At the time of discovery, the origin of the observed emission was not unambiguously identified, and follow-up observations have been performed to further investigate the nature of this γ-ray source.
Thérapie cellulaire pour le traitement de l’infarctus du myocarde (2009)

La therapie cellulaire est apparue ces dernieres annees comme etant une voie de recherche prometteuse pour le traitement de l’insuffisance cardiaque. Dans de nombreux modeles experimentaux d’infarctus du myocarde, l’injection intramyocardique de cellules d’origine tres diverse a permis d’ameliorer la fonction ventriculaire gauche. Cependant, cet effet serait independant de la formation de cellules contractiles et apparait plutot comme etant en rapport avec les proprietes paracrines pro-angiogeniques et anti-apoptotiques des cellules injectees. De nombreux essais cliniques ont ete realises chez l’homme. L’etude MAGIC, evaluant l’effet de l’injection intramyocardique de myoblastes au cours d’une chirurgie cardiaque, a conduit a des resultats decevants avec une absence d’efficacite sur la fonction ventriculaire gauche de ces patients. D’autres equipes ont etudie les potentialites therapeutiques de cellules mononucleees issues de la moelle osseuse administrees par voie endocoronaire. Les resultats de ces essais ont ete contradictoires mais plusieurs meta-analyses semblent conclure a un effet benefique statistiquement significatif bien que tres modeste chez des patients traites dans les suites d’un infarctus. Ces resultats montrent donc que de nombreux progres sont necessaires avant que la therapie cellulaire ne puisse devenir un traitement de routine en cardiologie. Cependant, la possibilite de recourir dans le futur a des cellules souches mesenchymateuses, des cellules embryonnaires, des cellules souches cardiaques ou a des cellules genetiquement modifiees ouvre des perspectives prometteuses.
Ultrasonic spectrum analysis for in vivo characterization of tumor microstructural changes in the evaluation of tumor response to chemotherapy using diagnostic ultrasound (2013)

Ultrasonic spectral analysis can detect changes in tumor microstructure after chemotherapy, and this will be helpful in the early evaluation tumor response to chemotherapy.
The Role of Ultrathin Coatings on the Interfacial Reactions at LiMn2O4 Surfaces (2015)

The spinel lithium manganese oxide (LiMn 2 O 4 , or LMO) is an established cathode technology for lithium ion batteries that suffers from a well-known phenomenon whereby Mn dissolves from the surface through the disproportionation reaction of Mn(III), and the subsequent deposition of Mn(II) on the anode. LMO can serve as a model system for exploring various mitigation strategies that will enrich our understanding of electrochemical corrosion at charged interfaces in general and may ultimately lead to better batteries. Consequently, the Center for Electrical Energy Science: Tailored Interfaces (CEES, a DOE-funded Energy Frontier Research Center), is exploring the influence of ultrathin coatings and surface treatments on the interfacial reactivity during lithiation reactions in LMO by combining advanced synthesis, in situ probes, and theory. As part of this effort, we have applied single-layer graphene coatings to LMO films, and discovered that these coatings improve capacity retention following electrochemical cycling. X-ray photoelectron spectroscopy (XPS) depth profiling, reveals that the graphene coating inhibits manganese depletion in the LMO film. Additionally, cross-sectional TEM demonstrates that a stable solid-electrolyte interphase layer is formed on the graphene coating, which screens the LMO from direct contact with the electrolyte, thereby inhibiting dissolution. Density functional theory (DFT) calculations have provided plausible mechanisms for this behavior. In a different effort, we have examined inert metal layers to prevent Mn dissolution. We found that 3-nm-thick Au shells prepared by electroless deposition could reduce Mn dissolution by up to 88%. Similarly, LiAlO 2 thin films deposited on LiNi 0.5 Mn 1.5 O 4 surfaces by atomic layer deposition can improve the electrochemical stability. The LiAlO 2 films are 100x more ionically conducting compared to Al 2 O 3 , and, consequently, provide a higher discharge capacity compared to Al 2 O 3 and allow for higher rates. Finally, we have explored the role of coherent coatings of a few nm-thick surface LiMn 2-x Ti x O 4 , deposited on LMO. This approach protects the bulk LMO from acid corrosion but is also electrochemically active and, therefore, maintains the ion and charge transport channels on the surface. The surface-doped LMO has &gt;2-fold improvement in electrochemical performance with respect to uncoated LMO in terms of cyclability and capacity at elevated temperature. In this presentation I will survey these ongoing research efforts into understanding the role of ultrathin coatings on interfacial reactions at LMO surfaces. This work was supported as part of the Center for Electrical Energy Storage: Tailored Interfaces, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences.
ChemInform Abstract: Structure and Synthesis of a Quercetin Glucoxyloside from Kalanchoe prolifera (Raym.‐Hamet). (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Stereo‐dependent dimerization, boiling points, diffusion coefficients, and dielectric constants of E/Z‐HFO‐1234ze (2021)

Abstract Stereo‐dependent properties of E‐ and Z‐HFO‐1234ze were investigated by density functional theory, extrapolated complete basis set limit, explicitly correlation, and symmetry‐adapted perturbation theory together with molecular dynamics simulations. The enthalpy of isomerization of E‐HFO‐1234ze to the Z isomer is 9.7 kJ/mol, showing the reverse cis effect . The HFO‐1234ze molecules interact predominantly via π‐π stacking of the double bonds and hydrogen bonding of CH⋯F. Although the E‐dimers involve less steric exchange‐repulsion, their binding energies are generally smaller by 2–6 kJ/mol than the Z‐dimers, in which the Coulombic multipole–multipole‐type electrostatic interactions and the long‐range dispersions are more profound. Local ordering in liquid occurs primarily via π stacking of the CHF‐end. The significant difference in boiling points of E‐ and Z‐HFO‐1234ze is clarified in terms of the dimerization free energy. Self‐diffusion coefficients and dielectric constants of HFO‐1234ze depend strongly on the E‐ and Z‐conformations.
F-probe fed broadband triangular patch antennas mounted on a finite ground plane (2004)

International audience
Multiobjective Optimization for Integrated Ground-Air-Space Networks: Current Research and Future Challenges (2021)

With space and aerial platforms deployed at different altitudes, integrated ground-air-space (IGAS) networks will have multiple vertical layers, hence forming a 3D structure. These 3D IGAS networks integrating both aerial and space platforms into terrestrial communications constitute a promising architecture for building fully connected global next-generation networks (NGNs).
ChemInform Abstract: TAUTOMERE PRAEFERENZ BEI GLYKOCYAMIDINEN (1971)

Abstract Zwölf Glykocyamidine werden dargestellt (7 davon erstmalig) und in bezug auf ihre pK a ‐Werte, die relativen Stabilitäten gegenüber alkalischer Hydrolyse, die relativen ′Geschwindigkeiten des basenkatalysierten D‐Austausches der Methylenprotonen im 5‐Ring und ihre UV‐ und NMR‐spektroskopischen Eigenschaften miteinander verglichen.
P2-S3.10 HSV-2 serologic testing and psychosocial harm: a systematic review (2011)

Background Serologic testing for herpes simplex virus type-2 (HSV-2) in persons without a history of genital herpes is currently not recommended partly due to the concern that HSV-2 diagnosis would lead to negative psychosocial sequelae, such as anxiety and depression. We conducted a systematic review to assess the evidence as to whether HSV-2 serologic testing among asymptomatic persons results in persistent negative psychosocial consequences. Methods Eight electronic databases and unpublished data sources were searched to identify studies measuring the psychosocial impact of HSV-2 serologic testing in persons without a history of genital herpes. To be included, studies had to test for HSV-2 using an HSV type-specific serologic test and to perform at least one psychosocial assessment of participants after they received HSV serologic results. We compared psychosocial responses in HSV-2 positive persons over time and vsHSV-2 negative persons (when available). Results Nine studies satisfied the inclusion criteria. Studies were published from the years 2000–2008 and were conducted in the USA (N=6), Australia (N=2), and the UK (N=1). In total, 1355 participants were included; 596 (44%) participants were HSV-2 positive, and of these 341 (57%) lacked prior history of genital herpes. Participants were recruited from a variety of settings (ie, STD clinics, HMO enrollees, college campuses). Follow-up ranged from immediately after diagnosis to 1 year afterwards. Seven studies reported that HSV-2 diagnosis by serologic test did not have a persistent negative impact on participants' mental health (anxiety, depression, self-esteem) or sexual attitude and satisfaction. Two studies reported a negative impact of testing; one found that 5 HSV-2 seropositive college students had increased distress 3 months post-testing as compared to HSV-2 negatives, and the other found self-reports of sexual undesirability up to one year after diagnosis. A genital herpes diagnosis was perceived as moderately severe for participants prior to testing; however, after HSV-2 testing, the perceived severity of a herpes diagnosis was lower among those testing HSV-2 positive. Conclusions Diagnosis of HSV-2 by type-specific serologic testing did not result in long-term psychosocial harm in most asymptomatic persons. Concerns about sustained emotional impact should not deter clinicians from testing individuals without a history of genital herpes for HSV-2.
Reference data generation of spray characteristics in relation to large 2-stroke marine diesel engines using a novel spray combustion chamber concept (2010)

The availability of appropriate reference data for combustion in large 2-stroke marine diesel engines is a prerequisite for the further development of Computation Fluid Dynamics tools. In order to enable the acquisition of such data at relevant physical dimensions (bore) and operational parameters (pressure, temperature), including flow characteristics (swirl) and the low fuel qualities involved, a novel experimental test facility has been realized. The core element is a disk-shaped constant volume spray combustion chamber of diameter 500 mm with peripheral injection into a swirl flow. Thermo- and fluid dynamic conditions at start of injection similar to those in real engines are achieved by feeding the chamber via inclined intake channels with pressurized and heated process gas provided by a pressure vessel/heat regenerating system. The chamber design includes comprehensive possibilities for optical access as well as various injector arrangement options and the injection system is prepared for running on typical marine fuels. Reference data for the fuel spray propagation has been acquired by means of shadow-imaging measurements: This involved the variation of key parameters such as gas pressure and temperature (up to 9 MPa, 930 K), number of injector orifices (1/2/5) and the spray orientation (10 deg counter to 40 deg co-swirl) relative to the gas flow. The spray propagation data collected at conditions representative of the operation of large 2-stroke marine diesel engines contributes to a better understanding of the underlying phenomena and enable the validation of simulation tools at such conditions. The new spray combustion chamber thus fills a clear gap and must be considered a reference setup for those applications.
Communication of Energy Price Information (2011)

This specification defines media types for representing the future
price of energy in JSON. It also defines a way for a client device,
such as an electric car, to discover a web server that can provide the
future pricing for local electrical energy. This will allow the client
device to make intelligent decisions about when to use energy. This
draft is a very early skeleton of a draft to start discussion around
this idea.
Targeted delivery of proapoptotic peptides to tumor-associated macrophages improves survival (2013)

Most current cancer therapies focus on killing malignant cells, but these cells are often genetically unstable and can become resistant to chemotherapy. Tumor-associated macrophages (TAMs) facilitate disease progression by promoting angiogenesis and tumor cell growth, as well as by suppressing the adaptive immune response. TAMs are therefore potential targets for adjuvant anticancer therapies. However, resident macrophages are critical to host defense, and preferential ablation of TAMs remains challenging. Macrophage activation is broadly categorized as classically activated, or M1, and alternatively activated, or M2, and TAMs in the tumor microenvironment have been shown to adopt the anti-inflammatory, M2-like phenotype. To date, there are no methods for specific molecular targeting of TAMs. In this work, we report the discovery of a unique peptide sequence, M2pep, identified using a subtractive phage biopanning strategy against whole cells. The peptide preferentially binds to murine M2 cells, including TAMs, with low affinity for other leukocytes. Confocal imaging demonstrates the accumulation of M2pep in TAMs in vivo after tail vein injection. Finally, tail vein injection of an M2pep fusion peptide with a proapoptotic peptide delays mortality and selectively reduces the M2-like TAM population. This work therefore describes a molecularly targeted construct for murine TAMs and provides proof of concept of this approach as an anticancer treatment. In addition, M2pep is a useful tool for murine M2 macrophage identification and for modulating M2 macrophages in other murine models of disease involving M2 cells.
Comparison of different reconstruction algorithms for image reconstruction in metamaterial aperture based imaging system (2015)

We investigate the performance of various image reconstruction algorithms for the design of a metamaterial aperture-based imaging system. The metamaterial imaging system consists of a transmitting metamaterial aperture panel and a receiving low-gain probe antenna. Imaging of a 3 cm resolution target is performed using a number of reconstruction algorithms, including pseudo-inverse, matched filter, conjugate gradient squares (CGS), and two-step iterative shrinkage/thresholding (TwIST) methods. The differing quality of the resulting reconstructed images is shown as a function of these reconstruction techniques.
Design of a Sub-mW 960-MHz UWB CMOS LNA (2006)

This paper presents a sub-mW ultra-wideband (UWB) fully differential CMOS low-noise amplifier (LNA) operating below 960 MHz for sensor network applications. By utilizing both nMOS and pMOS transistors to boost the transconductance, coupling the input signals to the back-gates of the transistors, and combining the common-gate and shunt-feedback topologies, the LNA achieves 13 dB of power gain, a 3.6 dB minimum noise figure, and -10 dBm of IIP3 with only 0.72 mW of power consumption from a 1.2 V supply
Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation (2006)

iTOL is available at http://itol.embl.de
The interplay of dendritic cell subsets in systemic lupus erythematosus (2002)

Dendritic cells (DC) control immunity and tolerance. Hence, we surmised that systemic lupus erythematosus (SLE), a systemic autoimmune disease with autoreactive T and B cells, might be due to DC alterations. Based on our findings, we are proposing a model of SLE where autoimmune responses are driven by unabated activation of myeloid DC through IFN-alpha produced by plasmacytoid DC. Thus, interplay between DC subsets might represent a key component of SLE pathogenesis.
Experimental Evaluation of Extended OpenFlow Deployment for High-Performance Optical Networks (2012)

Hybrid GMPLS-OpenFlow and novel Extended OpenFlow approaches are presented and experimentally evaluated on a converged packet and circuit switching setup. Extended OpenFlow agents, controllers and network application are developed &amp; their performance evaluated on commercial ROADMs for the first time.
Holocene Changes in Sea Level: Evidence in Micronesia (1967)

Investigation of 33 islands, scattered widely across the Caroline and Marshall Island groups in the Central Pacific revealed no emerged reefs in which corals had unquestionably formed in situ, or other direct evidence of postglacial high stands of sea level. Low unconsolidated rock terraces and ridges of reefflat islands, mostly lying between tide levels, were composed of rubble conglomerates; carbon-14 dating of 11 samples from the conglomerates so far may suggest a former slightly higher sea level (nine samples range between 1890 and 3450 and one approaches 4500 years ago). However, recent hurricanes have produced ridges of comparable height and material, and in the same areas relics from World War II have been found cemented in place. Thus these datings do not in themselves necessarily indicate formerly higher sea levels. Rubble tracts are produced by storms under present conditions without any change in datum, and there seems to be no compelling evidence that they were not so developed during various periods in the past.
Effect of cholate on H+-ATPase and other proteins of dog renal brush-border membrane (1993)

A short treatment of dog renal brush-border membrane vesicles (BBMV) with sodium cholate, followed by dialysis of the detergent, reorients the polarity of H(+)-ATPase in the membrane and exposes its ATP binding sites to the extravesicular space, as previously shown with pig BBMV. In cholate-pretreated vesicles, the H(+)-ATPase remains fully active, but is inserted under the reversed polarity in sealed vesicles. A large spontaneous N-ethylmaleimide-sensitive ATPase activity is thus observed, as well as a steep intravesicular acidification upon external ATP addition, two findings absent in native vesicles. The ability of nitrate plus ATP to dissociate the hydrolytic subunits ot the proton pump in cholate-pretreated vesicles, but not in native vesicles, demonstrates that most of the ATP binding subunits are accessible to ATP following cholate treatment. The sensitivity of the cytoplasmic domain of the H(+)-ATP activity to trypsin also confirms the reorientation of the enzyme in cholate-pretreated vesicles. The H(+)-ATPase and alkaline phosphatase remain largely associated with the membranes after the treatment with cholate, but gamma-glutamyltranspeptidase, aminopeptidase N, and neutral endopeptidase are largely solubilized. Upon dialysis of cholate, all these enzymes are in part reinserted in the membrane according to their original polarity. The reorientation process is however specific for the H(+)-ATPase. Cholate treatment does not increase the formation of inside-out vesicles. Thus the treatment with cholate really reorients the polarity of the H(+)-ATPase in vesicles and allows for study of the proton pumping capacity of vacuolar H(+)-ATPase of proximal tubules.
Hexaammineruthenium(II,III) and pentaamminedinitrogenruthenium(II). A Hartree-Fock-Slater study (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHexaammineruthenium(II,III) and pentaamminedinitrogenruthenium(II). A Hartree-Fock-Slater studyMary J. Ondrechen, Mary Jo Ondrechen, and D. E. EllisCite this: J. Am. Chem. Soc. 1981, 103, 7, 1656–1659Publication Date (Print):April 1, 1981Publication History Published online1 May 2002Published inissue 1 April 1981https://pubs.acs.org/doi/10.1021/ja00397a010https://doi.org/10.1021/ja00397a010research-articleACS PublicationsRequest reuse permissionsArticle Views103Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts