gebhart/patrepeval huggingface dataset:
Ligand Customization and DNA Functionalization of Gold Nanorods via Round-Trip Phase Transfer Ligand Exchange (2008)

Customizable ligand exchange of gold nanorods (NRs) is described. NRs are synthesized with the cationic surfactant cetyltrimethylammonium bromide (CTAB) which is exchanged with thiolated ligands that enable suspension in buffer. Exchange is achieved by a two phase extraction. First, CTAB is removed from the NR-CTAB by extracting the NRs into an organic phase via the ligand dodecanethiol (DDT). The NR-DDT are then extracted into an aqueous phase by mercaptocarboxylic acids (MCA), HS-(CH 2)n -COOH (n = 5, 10, and 15). Ligands can be further customized to thiolated poly(ethylene glycol), PEG MW (MW = 356, 5000, and 1000). Ligand-exchanged NRs (NR-MCA and NR-PEG(MW)) are stable in buffer, do not aggregate, and do not change size upon ligand exchange. They can be run in agarose gel electrophoresis with narrow bands, indicating uniform charge distribution and enabling quantitative analysis. DNA functionalization of NR-MCA is straightforward and quantifiable, with minimal nonspecific adsorption.
Minirailgun accelerator for plasma liner driven HEDP and magneto-inertial fusion experiments (2009)

Summary form only given. A spherical array of minirailgun plasma accelerators is a potential driver for forming imploding spherical plasma liners that can reach HEDP-relevant (~0.1 Mbar) pressures upon stagnation. The liners would be formed via merging of 30 or more dense, high Mach number plasma jets (n ~ 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">16-17</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> , M -10-35, v ~ 50-70 km/s, ¿ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">jet</sub> ~5 cm) in a spherically convergent geometry. The small (typically 1-2 cm square bore x 15-50 cm length) parallel-plate railguns with ceramic insulators would use pulsed injection of high-Z gas at the breech via fast opening valves to produce high density plasma jets with velocity in the 50-100 km/s range. Recent tests at HyperV using a single pulsed capillary discharge injecting into the minirailgun breech have achieved plasma densities in the bore approaching 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">18</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> , with densities in the jet plume exceeding 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">17</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> at velocities above 50 km/s. Total plasma jet mass in these 1 cm square bore tests has not yet been determined, but similar tests of an earlier 6 mm square bore 13 cm long device, with a roughly 3 ¿s, 100 kA current pulse using an aluminized mylar fuse starting from rest, yielded 90 ¿g of plasma at 50 km/s, and about 40 ¿g at 63 km/s. A modest scaleup of the railgun to a 2 cm square bore operating at longer pulse widths of 200-300 kA should be capable of accelerating a few thousand micrograms of high-Z gas (e.g. xenon) to above 50 km/s. This performance should be sufficient for reaching HEDP-relevant pressures.
Die Entlohnung des Managements beim (Leveraged) Management Buy-Out : Gestaltung aus ökonomischer und steuerlicher Sicht (2007)

Commercial photoplethysmography (PPG) sensors rely on the measurement of continuous-wave diffuse reflection signals (CW-DRS) to monitor heart rate. Using Monte Carlo modeling of light propagation in skin, we quantitatively evaluate the dependence of continuous-wave photoplethysmography (CW-PPG) in commercial wearables on source-detector distance (SDD). Specifically, when SDD increases from 0.5 mm to 3.3 mm, CW-PPG signal increases by roughly 846% for non-obese (NOB) skin and roughly 683% for morbidly obese (MOB) skin. Ultimately, we introduce the concept of time-of-flight PPG (TOF-PPG) which can significantly improve heart rate signals. Our model shows that the optimized TOF-PPG improves heart rate monitoring experiences by roughly 47.9% in NOB and 93.2% in MOB when SDD = 3.3 mm is at green light. Moving forward, these results will provide a valuable source for hypothesis generation in the scientific community to improve heart rate monitoring.
Pharmacology of retinotectal transmission in the goldfish: effects of nicotinic ligands, strychnine, and kynurenic acid (1987)

The goal of this study was to evaluate different neurotransmitters and their receptors that might be involved in retinotectal transmission in the goldfish. Sections of tectum were isolated and maintained in vitro while pharmacological agents were administered via the tissue bath. Field potentials were elicited by electrical stimulation of the optic nerve and recorded at 50 micron depth intervals, and profiles of current source densities (CSDs) were computed from the second spatial derivatives of these potentials. The preparations were treated with low [Ca2+]/high [Mg2+] media, various cholinergic agonists and antagonists, eserine, strychnine, or kynurenic acid, via the tissue bath. Prior to treatment, depth profiles of these in vitro field potentials and CSDs closely resembled those previously reported in vivo, including 2 prominent sink-source pairs with their sinks in the superficial optic neuropil, followed by a smaller and more prolonged sink-source pair of opposite polarity. These were rapidly and reversibly eliminated by low [Ca2+]/high [Mg2+] bathing media, and substantially reduced by 0.5 or 1.0 mM kynurenic acid. By contrast, d-tubocurarine (d-TC; up to 0.16 mM) reduced peak response amplitudes by less than 40%, eliminated the third sink-source pair, and more than doubled the duration of decay of sink-source pairs 1 and 2 in a concentration-dependent manner. Strychnine had a similar action to d-TC but was slightly more potent. The time course and amplitudes of responses were not much affected by the following nicotinic agonists or antagonists (concentrations in microM): mecamylamine, 50; dihydro-beta-erythroidine, 50; nicotine, 200; tetramethylammonium, 500; ACh (protected by eserine, 20), 200; alpha-bungarotoxin, 2 microM for 2.5 hr, and 0.4 microM for up to 10.5 hr; and lophotoxin, 32 microM for up to 94 min. Eserine (20 microM) and carbachol (200 microM) increased peak response amplitudes by up to 80% within 5-10 min, and amplitudes remained elevated during 20-33 min of continued treatment. The onset of the effects of d-TC, strychnine, and kynurenic acid began in 5-10 min and was completed in 30 min or less, indicating that test substances could adequately penetrate into the interior of the isolated sections of tectum. The failure of these cholinergic ligands to prevent postsynaptic responses indicates that excitatory retinotectal transmission does not depend on an intact nicotinic (or other cholinergic) system, as previously proposed. The action by kynurenic acid suggests the involvement of an excitatory amino acid neurotransmitter in retinotectal transmission.(ABSTRACT TRUNCATED AT 400 WORDS)
Crystal structure of human mitoNEET reveals distinct groups of iron–sulfur proteins (2007)

MitoNEET is a protein of unknown function present in the mitochondrial membrane that was recently shown to bind specifically the antidiabetic drug pioglizatone. Here, we report the crystal structure of the soluble domain (residues 32–108) of human mitoNEET at 1.8-Å resolution. The structure reveals an intertwined homodimer, and each subunit was observed to bind a [2Fe-2S] cluster. The [2Fe-2S] ligation pattern of three cysteines and one histidine differs from the known pattern of four cysteines in most cases or two cysteines and two histidines as observed in Rieske proteins. The [2Fe-2S] cluster is packed in a modular structure formed by 17 consecutive residues. The cluster-binding motif is conserved in at least seven distinct groups of proteins from bacteria, archaea, and eukaryotes, which show a consensus sequence of (hb)-C-X 1 -C-X 2 -(S/T)-X 3 -P-(hb)-C-D-X 2 -H, where hb represents a hydrophobic residue; we term this a CCCH-type [2Fe-2S] binding motif. The nine conserved residues in the motif contribute to iron ligation and structure stabilization. UV-visible absorption spectra indicated that mitoNEET can exist in oxidized and reduced states. Our study suggests an electron transfer function for mitoNEET and for other proteins containing the CCCH motif.
Prognosis in chronic fatigue syndrome: a prospective study on the natural course. (1996)

The improvement rate in patients with a relatively long duration of complaints is small. Psychological factors are related to improvement, especially cognitive factors.
Chronic Psychoemotional Stress Impairs Cannabinoid-Receptor-Mediated Control of GABA Transmission in the Striatum (2008)

Exposure to stressful events has a myriad of consequences in animals and in humans, and triggers synaptic adaptations in many brain areas. Stress might also alter cannabinoid-receptor-mediated transmission in the brain, but no physiological study has addressed this issue so far. In the present study, we found that social defeat stress, induced in mice by exposure to aggression, altered cannabinoid CB(1)-receptor-mediated control of synaptic transmission in the striatum. In fact, the presynaptic inhibition of GABAergic IPSCs induced by the cannabinoid CB(1) receptor agonist HU210 [(6aR)-trans-3-(1,1-dimethylheptyl)-6a,7,10,10a-tetrahydro-1-hydroxy-6,6-dimethyl-6H-dibenzo[b,d]pyran-9-methanol] was reduced after a single stressful episode and fully abolished after 3 and 7 d of stress exposure. Repeated psychoemotional stress also impaired the sensitivity of GABA synapses to endocannabinoids mobilized by group I metabotropic glutamate receptor stimulation, whereas the cannabinoid CB(1)-mediated control of glutamate transmission was unaffected by repeated exposure to an aggressor. Corticosteroids released in response to the activation of the hypothalamic-pituitary-adrenal axis played a major role in the synaptic defects observed in stressed animals, because these alterations were fully prevented by pharmacological blockade of glucocorticoid receptors and were mimicked by corticosterone injections. The recovery of stress-induced synaptic defects was favored when stressed mice were given access to a running wheel or to sucrose consumption, which function as potent natural rewards. A similar rescuing effect was obtained by a single injection of cocaine, a psychostimulant with strong rewarding properties. Targeting cannabinoid CB(1) receptors or endocannabinoid metabolism might be a valuable option to treat stress-associated neuropsychiatric conditions.
Promoter Analysis in the Human <I>SPRR</I> Gene Family (2004)

Protocols to study the regulation of a conserved multigene family (SPRR genes) during calcium-induced differentiation of cultured normal human keratinocytes (NHKs) are provided. Transfection of promoter-reporter (CAT or luciferase) constructs, combined with promoter truncation, can be used to study the expression of individual SPRR genes and to identify specific transcription factor binding sites. Interaction of regulatory factors with these control elements can be visualized and quantified by electro phoretic mobility shift analysis. Inclusion of specific antibodies in these experiments will identify the transcription factors involved in the observed mobility shift (supershift). A competitive electrophoretic mobility shift analysis, that is well suited to study the differential regulation of various SPRR members, is also described. These methods should be applicable to the study of other multigene families regulated during keratinocyte terminal differentiation.
SDX: a novel self-aligned technique and its application to high-speed bipolar LSIs (1988)

A novel polysilicon selective-deposition method named selective deposition by exdiffusion (SDX) and a self-aligned bipolar LSI process using the SDX process are presented. The SDX process makes it possible to selectively form polysilicon patterns on silicon oxide patterns but not on silicon nitride patterns, where these patterns are formed on a silicon substrate surface. Bipolar transistor self-alignment between the isolation pattern and the entire transistor active region can be achieved in a 0.85- mu m silicon island by the SDX process. A transistor with a 12-GHz cutoff frequency, an LCML gate with a propagation delay time of 59 ps/gate, and a 1-kb ECL (emitter-coupled logic) memory with an access time of 0.95 ns have been achieved.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Figure S6 from Stromal Reprogramming through Dual PDGFRα/β Blockade Boosts the Efficacy of Anti–PD-1 Immunotherapy in Fibrotic Tumors (2023)

&lt;p&gt;Supplementary Figure6&lt;/p&gt;
Soft pop-up mechanisms for micro surgical tools: Design and characterization of compliant millimeter-scale articulated structures (2016)

This paper introduces a manufacturing technique which enables the integration of soft materials and soft fluidic micro-actuators in the Pop-up book MEMS paradigm. Such a technique represents a promising approach to the design and fabrication of low cost and scalable articulated mechanisms provided with sensing capabilities and on-board actuation with potential applications in the field of minimally invasive surgery. Design and integration of soft components in the rigid-flex laminates is described along with the resulting soft pop-up mechanisms realized at different scales. Prototype characterization is presented, demonstrating forces and dexterity in a range suitable for surgical applications, as well as the possibility to integrate sensing capabilities. Based on these results, a multi-articulated robotic arm is fabricated and mounted on top of an endoscope model to provide a proof of concept of simple robotic mechanisms that could be useful in a surgical scenario.
Chronic neuropathic pain–like behavior and brain‐borne IL‐1β (2012)

Neuropathic pain in animals results in increased IL-1β expression in the damaged nerve, the dorsal root ganglia, and the spinal cord. Here, we discuss our results showing that this cytokine is also overexpressed at supraspinal brain regions, in particular in the contralateral side of the hippocampus and prefrontal cortex and in the brainstem, in rats with neuropathic pain-like behavior. We show that neuropathic pain degree and development depend on the specific nerve injury model and rat strain studied, and that there is a correlation between hippocampal IL-1β expression and tactile sensitivity. Furthermore, the correlations between hippocampal IL-1β and IL-1ra or IL-6 observed in control animals, are disrupted in rats with increased pain sensitivity. The lateralization of increased cytokine expression indicates that this alteration may reflect nociception. The potential functional consequences of increased IL-1β expression in the brain during neuropathic pain are discussed.
Extremes of Markov-additive Processes with One-sided Jumps, with Queueing Applications (2009)

Through Laplace transforms, we study the extremes of a continuous-time Markov-additive process with one-sided jumps and a finite-state background Markovian state-space, jointly with the epoch at which the extreme is ‘attained’. For this, we investigate discrete-time Markov-additive processes and use an embedding to relate these to the continuous-time setting. The resulting Laplace transforms are given in terms of two matrices, which can be determined either through solving a nonlinear matrix equation or through a spectral method. Our results on extremes are first applied to determine the steady-state buffer-content distribution of several single-station queueing systems. We show that our framework comprises many models dealt with earlier, but, importantly, it also enables us to derive various new results. At the same time, our setup offers interesting insights into the connections between the approaches developed so far, including matrix-analytic techniques, martingale methods, the rate-conservation approach, and the occupation-measure method. We also study networks of fluid queues, and show how the results on single queues can be used to find the Laplace transform of the steady-state buffer-content vector; it has a matrix quasi-product form. Fluid-driven priority systems also have this property.
Exacerbation of Thromboinflammation by Hyperglycemia Precipitates Cerebral Infarct Growth and Hemorrhagic Transformation (2017)

We show that hyperglycemia primes the thromboinflammatory cascade, thus, amplifying middle cerebral artery occlusion-induced DMT. DMT exacerbation in hyperglycemic rats impaired reperfusion and precipitated neurovascular damage, blood-brain barrier disruption, and hemorrhagic transformation. Our results designate DMT as a possible target for reduction of the deleterious impact of hyperglycemia in acute ischemic stroke.
Limited profit in predictable stock markets (2004)

It has been assumed that arbitrage profits are not possible in efficient markets, because future prices are not predictable. Here we show that predictability alone is not a sufficient measure of market efficiency. We instead propose to measure inefficiencies of markets in terms of the maximal profit an ideal trader can take out from a market. In a stock market model with an evolutionary selection of agents this method reveals that the mean relative amount of realizable profits $P$ is very limited and we find that it decays with rising number of agents in the markets. Our results show that markets may self-organize their collective dynamics such that it becomes very sensitive to profit attacks which demonstrates that a high degree of market efficiency can coexist with predictability.
On the relation of reachability to minimum cost optimal control (2003)

Questions of reachability for continuous and hybrid systems can be formulated as optimal control or game theory problems, whose solution can be characterised using variants of the Hamilton-Jacobi-Bellman or Isaacs partial differential equations. This paper establishes a link between reachability and invariance problems and viscosity solutions of a Hamilton-Jacobi partial differential equation, developed to address optimal control problems where the cost function is the minimum of a function of the state over a given horizon. The form of the resulting partial differential equation (continuity of the Hamiltonian and simple boundary conditions) makes this approach especially attractive from the point of view of numerical computation.
Fluid and hemodynamic management in hemodialysis patients: challenges and opportunities (2019)

Fluid volume and hemodynamic management in hemodialysis patients is an essential component of dialysis adequacy. Restoring salt and water homeostasis in hemodialysis patients has been a permanent quest by nephrologists summarized by the 'dry weight' probing approach. Although this clinical approach has been associated with benefits on cardiovascular outcome, it is now challenged by recent studies showing that intensity or aggressiveness to remove fluid during intermittent dialysis is associated with cardiovascular stress and potential organ damage. A more precise approach is required to improve cardiovascular outcome in this high-risk population. Fluid status assessment and monitoring rely on four components: clinical assessment, non-invasive instrumental tools (e.g., US, bioimpedance, blood volume monitoring), cardiac biomarkers (e.g. natriuretic peptides), and algorithm and sodium modeling to estimate mass transfer. Optimal management of fluid and sodium imbalance in dialysis patients consist in adjusting salt and fluid removal by dialysis (ultrafiltration, dialysate sodium) and by restricting salt intake and fluid gain between dialysis sessions. Modern technology using biosensors and feedback control tools embarked on dialysis machine, with sophisticated analytics will provide direct handling of sodium and water in a more precise and personalized way. It is envisaged in the near future that these tools will support physician decision making with high potential of improving cardiovascular outcome.
Performance Evaluation and Control Strategy Comparison of Supercapacitors for a Hybrid Electric Vehicle (2019)

Electrification of powertrain system is a great technical progress of traditional vehicle, leading to a significant reduction of fuel consumption and emission pollution. Energy storage system (ESS) normally consisting of batteries is a key component of an electric vehicle or hybrid electric vehicle. An ESS can recover braking energy during the regenerative braking process. Currently, lithium-ion batteries are the main energy storage device due to their high energy density. However, sometimes, a sudden large increase of operation current is required during acceleration or regenerative braking processes, which will jeopardize the operation life of batteries. A supercapacitor takes advantage of high power density and can tolerate large current in a short time. Application of supercapacitor in an ESS can reduce the peak current of batteries effectively, and the life time of batteries can be extended. Meanwhile, the braking energy can also be recovered sufficiently. Supercapacitors can be used solely in some hybrid electric vehicles. In this chapter, the application of supercapacitors in electric vehicles or hybrid electric vehicles is reviewed briefly. Then, the performance of a series hybrid transit bus, which uses a compressed natural gas engine and supercapacitors as power sources, is analyzed.
QMP-MVIA: a message passing system for Linux clusters with gigabit Ethernet mesh connections (2005)

Recent progress in performance coupled with a decline in price for copper-based gigabit Ethernet (GigE) interconnects makes them an attractive alternative to expensive high speed network interconnects (NIC) when constructing Linux clusters. However traditional message passing systems based on TCP for GigE interconnects cannot fully utilize the raw performance of today's GigE interconnects due to the overhead of kernel involvement and multiple memory copies during sending and receiving messages. The overhead is more evident in the case of mesh connected Linux clusters using multiple GigE interconnects in a single host. We present a general message passing system called QMP-MVIA (QCD Message Passing over M-VIA) for Linux clusters with mesh connections using GigE interconnects. In particular, we evaluate and compare the performance characteristics of TCP and M-VIA (an implementation of the VIA specification) software for a mesh communication architecture to demonstrate the feasibility of using M-VIA as a point-to-point communication software, on which QMP-MVIA is based. Furthermore, we illustrate the design and implementation of QMP-MVIA for mesh connected Linux clusters with emphasis on both point-to-point and collective communications, and demonstrate that QMP-MVIA message passing system using GigE interconnects achieves bandwidth and latency that are not only better than systems based on TCP but also compare favorably to systems using some of the specialized high speed interconnects in a switched architecture at much lower cost.