gebhart/patrepeval huggingface dataset:
Supramolecular Surface Coatings with Acetylated Polyrotaxane-Based Triblock Copolymers for Thermal Regulation of Cell Adhesion and Fabrication of Cell Sheets (2022)

Polyrotaxanes (PRXs) containing acetylated α-cyclodextrins exhibit a temperature-dependent phase transition in aqueous solutions across their lower critical solution temperature (LCST) of approximately 26.6 °C. To gain insights into the interactions of acetylated PRXs (Ac-PRXs) with biological components, thermoresponsive supramolecular surfaces were prepared by coating tissue culture polystyrene (TCPS) surfaces with Ac-PRX triblock copolymers, and their surface properties across the LCST were evaluated. The wettability and protein adsorption of Ac-PRX-coated surfaces changed significantly between 10 and 37 °C, whereas the uncoated TCPS and unmodified PRX-coated surfaces did not alter the wettability and protein adsorption at 10 and 37 °C. The adhesion, proliferation, morphology, and adhesion strength of NIH/3T3 cells on Ac-PRX-coated surfaces were found to be similar to those of the uncoated and unmodified PRX-coated surfaces. However, the adhesion strength of NIH/3T3 cells on Ac-PRX-coated surfaces decreased drastically at 10 °C. Consequently, the cells spontaneously detached from the Ac-PRX-coated surfaces without enzymatic treatment. Additionally, when incubating confluent cells at 10 °C, the cells detached from Ac-PRX-coated surfaces as cell sheets while retaining extracellular matrix proteins. The findings of this study provide new directions for the design of thermoresponsive supramolecular biointerfaces for applications in bioseparation and cell manipulation.
Modulation of human polymorphonuclear neutrophil function by macrolides: preliminary data concerning dirithromycin (1993)

Polymorphonuclear neutrophils (PMN) play a prominent role in the host response to infectious diseases. One major bactericidal mechanism used by these cells is the production of reactive oxygen species during what is referred to as the oxidative burst. However, excessive oxidant generation can also be involved in cell and tissue damage associated with severe inflammatory reactions. Macrolide antibiotics are able to penetrate and concentrate within phagocytes and have been successfully used to treat infections due to facultative intracellular pathogens. However, intracellular accumulation of macrolides with possible alkalinization of cellular compartments may interfere with normal cell function. In-vitro and ex-vivo data suggest that macrolides affect various phagocytic functions. This paper presents an overview of the published data concerning the modulation of neutrophil function by macrolides. Preliminary data concerning the in-vitro modulation of the neutrophil oxidative burst by dirithromycin and its metabolite, erythromycylamine, are also discussed.
RETRACTED: Endurance exercise rescues progeroid aging and induces systemic mitochondrial rejuvenation in mtDNA mutator mice (2011)

A causal role for mitochondrial DNA (mtDNA) mutagenesis in mammalian aging is supported by recent studies demonstrating that the mtDNA mutator mouse, harboring a defect in the proofreading-exonuclease activity of mitochondrial polymerase gamma, exhibits accelerated aging phenotypes characteristic of human aging, systemic mitochondrial dysfunction, multisystem pathology, and reduced lifespan. Epidemiologic studies in humans have demonstrated that endurance training reduces the risk of chronic diseases and extends life expectancy. Whether endurance exercise can attenuate the cumulative systemic decline observed in aging remains elusive. Here we show that 5 mo of endurance exercise induced systemic mitochondrial biogenesis, prevented mtDNA depletion and mutations, increased mitochondrial oxidative capacity and respiratory chain assembly, restored mitochondrial morphology, and blunted pathological levels of apoptosis in multiple tissues of mtDNA mutator mice. These adaptations conferred complete phenotypic protection, reduced multisystem pathology, and prevented premature mortality in these mice. The systemic mitochondrial rejuvenation through endurance exercise promises to be an effective therapeutic approach to mitigating mitochondrial dysfunction in aging and related comorbidities.
Spectres de vibration et structure de composés de coordination nitrés du platine IV (1965)

Étude du spectre d’absorption intrarouge, entre 300 et 1 800 cm-1 , de composés halogénonitrés, halogénodiamminonitrés, tétramminonitrés du PlatineIV. Distinction des isomères. Discussion sur la formule de [PtIV(NH3)4(NO2)2] (NO3)2. État du groupe NO2 dans les composés du Platine oxydé.
Thermal Energy Storage Case Studies (2010)

This chapter contains sections titled: Introduction Ice CTES Case Studies Ice-Slurry CTES Case Studies Chilled Water CTES Case Studies PCM-Based CTES Case Studies PCM-Based Latent TES for Heating Case Studies Sensible TES Case Studies Other Case Studies Concluding Remarks References
The Differential Axonal Degradation of Ret Accounts for Cell-Type-Specific Function of Glial Cell Line-Derived Neurotrophic Factor as a Retrograde Survival Factor (2010)

Glial cell line-derived neurotrophic factor (GDNF) is a neuronal growth factor critical for the development and maintenance of central and peripheral neurons. GDNF is expressed in targets of innervation and provides support to several populations of large, projection neurons. To determine whether GDNF promotes retrograde survival over long axonal distances to cell bodies, we used a compartmentalized culture system. GDNF supported only modest and transient survival of postnatal sympathetic neurons when applied to their distal axons, in contrast to dorsal root ganglion (DRG) sensory neurons in which GDNF promoted survival equally well from either distal axons or cell bodies. Ret, the receptor tyrosine kinase for GDNF, underwent rapid proteasomal degradation in the axons of sympathetic neurons. Interestingly, the level of activated Ret in DRG neurons was sustained in the axons and also appeared in the cell bodies, suggesting that Ret was not degraded in sensory axons and was retrogradely transported. Pharmacologic inhibition of proteasomes only in the distal axons of sympathetic neurons caused an accumulation of activated Ret in both the axons and cell bodies during GDNF stimulation. Furthermore, exposure of the distal axons of sympathetic neurons to both GDNF and proteasome inhibitors, but neither one alone, promoted robust survival, identical to GDNF applied directly to the cell bodies. This differential responsiveness of sympathetic and sensory neurons to target-derived GDNF was attributable to the differential expression and degradation of the Ret9 and Ret51 isoforms. Therefore, the local degradation of Ret in axons dictates whether GDNF family ligands act as retrograde survival factors.
Cooling systems and methods for thermoelectric power generation (2023)

Systems and methods for cooling a power generation working fluid are disclosed that reduce the amount of cooling fluid used. These systems and methods save on water usage in the generation of power by thermoelectric power generation systems.
Graves' ophthalmopathy: a preventable disease? (2002)

Most patients with Graves' disease have some degree of ocular involvement, but only 3-5% of them develop severe ophthalmopathy (1). The reasons why only such a minority of patients with Graves' disease have severe expression of the ophthalmopathy remain to be elucidated. One possible explanation is that non-severe ophthalmopathy and severe ophthalmopathy are two different disorders with different genetic backgrounds; alternatively, they might be part of a spectrum of different conditions ranging from absent ocular involvement to most severe ophthalmopathy. In this case, external variables (i.e. environmental factors) must contribute to the nature of the expression of the disease. How important are they? How far can our intervention on environmental factors go towards reducing the risk of progression of the ophthalmopathy? In other words, to which extent, if any, is Graves' ophthalmopathy preventable? The aim of this mini-review is to address the above issues.
Improvement in Cell-Mediated Immune Function during Potent Anti-Human Immunodeficiency Virus Therapy with Ritonavir plus Saquinavir (1998)

Inhibiting human immunodeficiency virus (HIV) replication with potent antiretroviral therapy may result in improved immune function, and this may lead to favorable outcomes, independent of changes in CD4+ lymphocyte count. The effect of combination protease inhibitor therapy (ritonavir plus saquinavir) on functional measures of cell-mediated immunity in 41 HIV-infected patients from one center of a multicenter trial was investigated. After 24 weeks, median plasma virus load decreased from 4.74 log10 copies/mL to below the detection limit of the assay (2.30 log10), and mean CD4+ lymphocyte count increased from 284 cells/microL to 413 cells/microL. Proliferative responses to phytohemagglutinin developed in 21 of 34 patients in whom responses were absent at baseline. Increases were observed in interleukin-2, -12, and -10 production and in the expression of CD28 on CD8+ lymphocytes. Initiation of potent anti-HIV therapy results in a degree of immune restoration, suggesting that HIV-induced immune suppression is a dynamic and potentially reversible process.
A penta-co-ordinated aluminate dimer; X-ray crystal structure (1985)

M. C. Cruickshank and L. S. D. Glasser, J. Chem. Soc., Chem. Commun., 1985, 84 DOI: 10.1039/C39850000084
Preservation of Mycoplasma Strains by Freezing in Liquid Nitrogen and by Lyophilization with Sucrose (1970)

Procedures for maintaining Mycoplasma strains are described. Liquid nitrogen storage provided an adequate means for keeping stock cultures stable. Over 74 strains of approximately 26 species have been preserved in this way, some for as long as 9 years. Mycoplasma strains can also be recovered satisfactorily from the freeze-dried state when the procedure described includes the use of 12% (v/v) sucrose as an additive. Fifteen strains representing 12 or more species were subjected to a freeze-drying program with and without sucrose added to concentrated cell suspensions in growth medium. Cell counts indicate improved survival with sucrose.
A Hardware Digital Controller for Undergraduate DDC Experiments (1975)

This paper describes a digital control laboratory facility which teaches the central concepts of digital control without requiring a background in digital systems and/or machine language programming. The heart of the laboratory is a flexible hardware digital controller replacing the traditional analog control components in a D. C. servo system. The digital controller can be adjusted to provide versions of the standard PID control algorithm with adjustable word length and computation delay. Through a series of graded experiments this hardware can provide: (1) an introduction to digital control concepts and components, (2) an introduction to digital signal processing, (3) experience with basic DDC algorithms, (4) experience with problems encountered in the choice of sampling rate and digital word length, and (5) experience in working with and trouble-shooting an operating digital control system. Since one controller can be provided for each laboratory station the student has considerable freedom to learn by doing at his own rate.
Gene Expression Microarrays: Improvements and Applications Towards Agricultural Gene Discovery (2001)

In agriculture, the associations between genes and resulting traits revealed by high throughput approaches such as transcription profiling could be used to select more environmentally friendly chemicals for plant protection and to develop plants with increased grain yields and better nutrition value, with more resistance to diseases and tolerance to abiotic stress. However, one of the major challenges to apply such approaches is the limited genomic information for most of the very diversified crop species. We developed multiple strategies and platform technologies to address this issue. Here we report our improvements of these technologies towards large-scale transcription profiling and their applications in agricultural gene discovery.
Odorant binding proteins from Hermetia illucens: potential sensing elements for detecting volatile aldehydes involved in early stages of organic decomposition (2022)

Organic decomposition processes, involving the breakdown of complex molecules such as carbohydrates, proteins and fats, release small chemicals known as volatile organic compounds (VOCs), smelly even at very low concentrations, but not all readily detectable by vertebrates. Many of these compounds are instead detected by insects, mostly by saprophytic species, for which long-range orientation towards organic decomposition matter is crucial. In the present work the detection of aldehydes, as an important measure of lipid oxidation, has been possible exploiting the molecular machinery underlying odour recognition in<i>Hermetia illucens</i>(Diptera: Stratiomyidae). This voracious scavenger insect is of interest due to its outstanding capacity in bioconversion of organic waste, colonizing very diverse environments due to the ability of sensing a wide range of chemical compounds that influence the choice of substrates for ovideposition. A variety of soluble odorant binding proteins (OBPs) that may function as carriers of hydrophobic molecules from the air-water interface in the antenna of the insect to the receptors were identified, characterised and expressed. An OBP-based nanobiosensor prototype was realized using selected OBPs as sensing layers for the development of an array of quartz crystal microbalances (QCMs) for vapour phase detection of selected compounds at room temperature. QCMs coated with four recombinant<i>H. illucens</i>OBPs (HillOBPs) were exposed to a wide range of VOCs indicative of organic decomposition, showing a high sensitivity for the detection of three chemical compounds belonging to the class of aldehydes and one short-chain fatty acid. The possibility of using biomolecules capable of binding small ligands as reversible gas sensors has been confirmed, greatly expanding the state-of the-art in gas sensing technology.
Étude expérimentale de l'autoxidation n-dodecane et 124-trimethylbenzene (2019)

International audience
Remarkable reversal of melting point alternation by co-crystallization (2010)

The pronounced melting point alternation of α,ω-alkanedicarboxylic acids (HOOC–(CH2)n−2–COOH, n = 4–10) is maintained in co-crystals of the same acids with 1,2-bis(4-pyridyl)ethane (BPA), which contains an even number of carbon atoms in the chain, and is reversed in co-crystals with 1,2-bis(4-pyridyl)propane (BPP), which contains an odd number of carbon atoms in the aliphatic chain.
Application of Fuzzy Logic PSS to Enhance Transient Stability in Large Power Systems (2006)

The paper presents an application of fuzzy logic power system stabilizers (FLPSS) to enhance transient stability in large electric power systems. A two-stage technology of FLPSS adaptation is considered taking into account the real conditions of a power system. Self-organizing artificial neural network (ANN) is used for clusterization of the test disturbances. A genetic algorithm (GA) is applied to tuning parameters of FLPSS. ANN is used on-line to adapt FLPSS to changes in operating conditions. The studies have been conducted using the models of a 14-bus multi-machine power system and regional Siberian power system as the study cases. The results obtained are presented.
Compact LTCC filter with vertically end-coupled resonators (2017)

A compact narrow-band low temperature co-fíred ceramic (LTCC) filter with a fractional bandwidth of 3% is proposed. The proposed filter consists of five cascaded half-wavelength resonators vertically arrayed on each LTCC layer. End-coupling between adjacent resonators is precisely controlled by rectangle-shaped slot on intermediate ground layer. The overall size of the filter is only 8 × 6 × 0.89 mm and a size reduction of 80% is achieved compared with a planar one. Measured S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">11</sub> and S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">21</sub> are better than -14 and -2.5 dB in the passband, respectively.
US of the snapping iliopsoas tendon. (1996)

US is a useful dynamic noninvasive technique for the diagnosis of snapping iliopsoas tendon.
Sustainable and Continuous Synthesis of Enantiopure l‐Amino Acids by Using a Versatile Immobilised Multienzyme System (2017)

The enzymatic synthesis of α-amino acids is a sustainable and efficient alternative to chemical processes, through which achieving enantiopure products is difficult. To more address this synthesis efficiently, a hierarchical architecture that irreversibly co-immobilises an amino acid dehydrogenase with polyethyleneimine on porous agarose beads has been designed and fabricated. The cationic polymer acts as an irreversible anchoring layer for the formate dehydrogenase. In this architecture, the two enzymes and polymer colocalise across the whole microstructure of the porous carrier. This multifunctional heterogeneous biocatalyst was kinetically characterised and applied to the enantioselective synthesis of a variety of canonical and noncanonical α-amino acids in both discontinuous (batch) and continuous modes. The co-immobilised bienzymatic system conserves more than 50 % of its initial effectiveness after five batch cycles and 8 days of continuous operation. Additionally, the environmental impact of this process has been semiquantitatively calculated and compared with the state of the art.
Colorization by Patch-Based Local Low-Rank Matrix Completion (2015)

Colorization aims at recovering the original color of a monochrome image from only a few color pixels. A state-of-the-art approach is based on matrix completion, which assumes that the target color image is low-rank. However, this low-rank assumption is often invalid on natural images. In this paper, we propose a patch-based approach that divides the image into patches and then imposes a low-rank structure only on groups of similar patches. Each local matrix completion problem is solved by an accelerated version of alternating direction method of multipliers (ADMM), and each AD-MM subproblem is solved efficiently by divide-and-conquer. Experiments on a number of benchmark images demonstrate that the proposed method outperforms existing approaches.
Study on Error-Detecting Approach for Fault Tolerance Recomputing Oriented Parallel Digital Terrain Analysis (2014)

In recent years, the research of parallel digital terrain analysis has become a hot spot. Using the parallel computing technology to solve data intensive problems, and it has become a development trend in digital terrain analysis. On the other hand, with the development of hardware technology and new applications, how to ensure the reliability of the computing results is a one of the key problems. We can improve the system's ability to provide the right service by adopting fault tolerance technology properly. The paper proposes a parallel error-detecting approach which is based on a mixed mode of shared memory and distributed memory. By adopting the parallel comparison, it can improve the efficiency of fault tolerance recomputing. According to the error-detecting analysis of slope algorithm, it proves the effectiveness of error-detecting approach based on fault-tolerant recomputing and achieves minor overhead.
Congenital CNS Hypomyelination in theFig4Null Mouse Is Rescued by Neuronal Expression of the PI(3,5)P2PhosphataseFig4 (2011)

The plt (pale tremor) mouse carries a null mutation in the Fig4(Sac3) gene that results in tremor, hypopigmentation, spongiform degeneration of the brain, and juvenile lethality. FIG4 is a ubiquitously expressed phosphatidylinositol 3,5-bisphosphate phosphatase that regulates intracellular vesicle trafficking along the endosomal-lysosomal pathway. In humans, the missense mutation FIG4(I41T) combined with a FIG4 null allele causes Charcot-Marie-Tooth 4J disease, a severe form of peripheral neuropathy. Here we show that Fig4 null mice exhibit a dramatic reduction of myelin in the brain and spinal cord. In the optic nerve, smaller-caliber axons lack myelin sheaths entirely, whereas many large- and intermediate-caliber axons are myelinated but show structural defects at nodes of Ranvier, leading to delayed propagation of action potentials. In the Fig4 null brain and optic nerve, oligodendrocyte (OL) progenitor cells are present at normal abundance and distribution, but the number of myelinating OLs is greatly compromised. The total number of axons in the Fig4 null optic nerve is not reduced. Developmental studies reveal incomplete myelination rather than elevated cell death in the OL linage. Strikingly, there is rescue of CNS myelination and tremor in transgenic mice with neuron-specific expression of Fig4, demonstrating a non-cell-autonomous function of Fig4 in OL maturation and myelin development. In transgenic mice with global overexpression of the human pathogenic FIG4 variant I41T, there is rescue of the myelination defect, suggesting that the CNS of CMT4J patients may be protected from myelin deficiency by expression of the FIG4(I41T) mutant protein.
Synthesis and Biological Evaluation of Rhein-Based MRI Contrast Agents for in Vivo Visualization of Necrosis (2018)

Early and accurate assessment of therapeutic response to anticancer therapy plays an important role in determining treatment planning and patient management in clinic. Magnetic rseonance imaging (MRI) of necrosis that occurs after cancer therapies provides chances for that. Here, we reported three novel MRI contrast agents, GdL<sub>1</sub>, GdL<sub>2</sub>, and GdL<sub>3</sub>, by conjugating rhein with gadolinium 2-[4,7,10-tris(carboxymethyl)-1,4,7,10-tetraazacyclododec-1-yl]acetic acid (Gd-DOTA) through different linkers. The T1 relaxivities of three probes (7.28, 7.35, and 8.03 mM<sup>-1</sup> s<sup>-1</sup>) were found to be higher than that of Gd-DOTA (4.28 mM<sup>-1</sup> s<sup>-1</sup>). Necrosis avidity of GdL<sub>1</sub> was evaluated on the rat models of reperfused liver infarction (RLI) by MRI, which showed an increase of T1-weighted contrast between necrotic and normal liver during 0.5-12 h. Besides, L<sub>1</sub> was also labeled with <sup>64</sup>Cu to assess its necrosis avidity on rat models of RLI and muscle necrosis (MN) by a γ-counter. The uptakes of <sup>64</sup>CuL<sub>1</sub> in necrotic liver and muscle were higher than those in normal liver and muscle ( P < 0.05). Then, the ability of GdL<sub>1</sub> to assess therapeutic response was tested on rats bearing Walker 256 breast carcinoma injected with a vascular disrupting agent CA4P by MR imaging. The signal intensity of tumoral necrosis was strongly enhanced, and the contrast ratio between necrotic and viable tumor was 1.63 ± 0.11 at 3 h after administration of GdL<sub>1</sub>. Besides, exposed DNA in necrosis cells may be an important mechanism of three probes targeting to necrosis cells. In summary, GdL<sub>1</sub> may serve as a promising MRI contrast agent for accurate assessment of treatment response.
Intracranial Arteries in Individuals with the Elastin Gene Hemideletion of Williams Syndrome (2013)

Despite the elastin haploinsufficiency, the proximal intracranial arteries in Williams syndrome preserve normal patency.