gebhart/patrepeval huggingface dataset:
A lipase–glucose oxidase system for the efficient oxidation of N-heteroaromatic compounds and tertiary amines (2016)

In this work, a lipase–glucose oxidase system has been designed and proven to be an efficient system for the oxidation of <italic>N</italic>-heteroaromatic compounds and tertiary amines.
Isolation and Structure-Function Studies of a Glucagon-Like Peptide 1 Receptor from Goldfish Carassius auratus: Identification of Three Charged Residues in Extracellular Domains Critical for Receptor Function (2002)

A better understanding of the molecular mechanism of ligand-receptor interaction of glucagon-like peptide 1 (GLP-1) receptors (GLP-1Rs) is useful for the design of potent GLP-1 analogs that could potentially be used as a treatment for diabetic patients. Changes in the ligand and receptor sequences during evolution provide invaluable clues to evaluate the functional motifs of the receptor that are responsible for ligand interaction. For these reasons, in the present study, we have isolated and functionally characterized a GLP-1R from goldfish. Its amino acid sequence shows 50.8% and 52.3% identity with the human glucagon (hGLU) and GLP-1Rs, respectively, and 84.1% with the zebrafish GLP-1R (the only other GLP-1R isolated from teleost fish). Peptides that are structurally different from goldfish (gf)GLP-1, such as gfGLU and hGLU and human GLP-1 (7-36)amide, are also capable of stimulating this receptor, albeit with lower potencies than gfGLP-1. gfGLP-1 stimulates the formation of cAMP through the recombinant gfGLP-1R with EC(50) = 0.18 nM, whereas EC(50) values for gfGLU, human GLP-1 (7-36)amide, and hGLU are 0.53 nM, 0.9 nM, and 1.2 nM, respectively. These results indicate that the gfGLP-1R is structurally more flexible than its mammalian counterpart and that its binding pocket can accommodate a wider spectrum of peptide ligands. Previous studies demonstrated that the charged residues in the extracellular domains of mammalian GLP-1R, particularly those found in the N-terminal domain and the first exoloop, are important for ligand binding. We investigated the roles of the conserved charged residues in the function of the gfGLP-1R. Eleven mutant receptors were constructed, and the effects of mutations were determined by functional assays. Our results demonstrated that three charged residues (D(113), R(197), and D(205)) present in the extracellular domains are critical for receptor function.
Feeding the second screen: semantic linking based on subtitles (2013)

Television is changing. Increasingly, broadcasts are consumed interactively. This allows broadcasters to provide consumers with additional background information that they may bookmark for later consumption. To support this type of functionality, we consider the task of linking a textual streams derived from live broadcasts to Wikipedia. While link generation has received considerable attention in recent years, our task has unique demands that require an approach that needs to (i) be high-precision oriented, (ii) perform in real-time, (iii) work in a streaming setting, and (iv) typically, with a very limited context. We propose a learning to rerank approach that significantly improves over a strong baseline in terms of effectiveness and whose processing time is very short. We extend this approach, leveraging the streaming nature of the textual sources that we link by modeling context as a graph. We show how our graph-based context model further improves effectiveness. For evaluation purposes we create a dataset of segments of television subtitles that we make available to the research community.
Impedance Spectroscopy of Narrow Band Gap III-V MOS Systems (2016)

One of the main research challenges facing future developments in areas such as nano-electronics, energy storage, the Internet of Things and communications is the need to create devices which are more energy efficient. From the perspective of both energy efficiency, and the integration of RF functions on a silicon platform, there is a growing interest in the use of high electron mobility channels, such as In&amp;lt;sub&amp;gt;x&amp;lt;/sub&amp;gt;Ga&amp;lt;sub&amp;gt;1-x&amp;lt;/sub&amp;gt;As, in conjunction with high dielectric constant (high-&amp;lt;i&amp;gt;k&amp;lt;/i&amp;gt;) gate oxides for &amp;lt;i&amp;gt;n&amp;lt;/i&amp;gt;-channel Metal-Oxide-Semiconductor Field Effect Transistors (MOSFETs) in FinFET structures and in 3D integration schemes. The understanding and control of electrically active defect states at the high-&amp;lt;i&amp;gt;k&amp;lt;/i&amp;gt;/In&amp;lt;sub&amp;gt;x&amp;lt;/sub&amp;gt;Ga&amp;lt;sub&amp;gt;1-x&amp;lt;/sub&amp;gt;As interface will be essential for the successful implementation of high mobility channel materials in MOSFET or tunnel FET configurations. The objective of this presentation will be to provide an overview of the current understanding of the density and distribution of electrically active defects at the high-&amp;lt;i&amp;gt;k&amp;lt;/i&amp;gt;/In&amp;lt;sub&amp;gt;x&amp;lt;/sub&amp;gt;Ga&amp;lt;sub&amp;gt;1-x&amp;lt;/sub&amp;gt;As interface, along with initial investigations of MOS structures on GaSb and InGaSb surfaces. This presentation will also consider the issue of capacitance-voltage (CV) hysteresis in high-&amp;lt;i&amp;gt;k&amp;lt;/i&amp;gt;/InGaAs and high-&amp;lt;i&amp;gt;k&amp;lt;/i&amp;gt;/(In)GaSb MOS structures, along with methods to reduce the level of hysteresis charge trapping instabilities. Finally, the characteristic signatures of capacitance and conductance, which indicate genuine surface inversion of a narrow energy gap III-V MOS structure in inversion will be discussed.
Physiological Modulation of Eustachian Tube Function (1987)

The effect of changing body position on the Eustachian tube opening time (TOT) and nasal conductance (NC) was investigated in 5 subjects. Eustachian tube function was evaluated using a sonotubometric technique and NC was determined by anterior or posterior rhinomanometry. The results showed that both the TOT and NC were decreased by changing the body position from erect to recumbent. On a different day, Eustachian tube function and rhinomanometry tests were repeated every 30 min over a 5-7 h period. The results documented a nasal cycle in all subjects. Moreover, TOT tracked the ipsilateral NC in 3 subjects and the contralateral NC in 2 subjects. Since TOT measures mucosal venous congestion, these findings suggest that the tubal mucosa also experiences autonomically modulated cyclic fluctuations.
Metallic oxide nanoparticles stimulate blood coagulation independent of their surface charge (2013)

Positively charged metallic oxides prevent blood coagulation whereas negatively charged metallic oxides are thrombogenic. This study was performed to examine whether this effect extends to metallic oxide nanoparticles. Oscillation shear rheometry was used to study the effect of zinc oxide and silicon dioxide nanoparticles on thrombus formation in human whole blood. Our data show that oscillation shear rheometry is a sensitive and robust technique to analyze thrombogenicity induced by nanoparticles. Blood without previous contact with nanoparticles had a clotting time (CT) of 16.7 ± 1.0 min reaching a maximal clot strength (CS) of 16 ± 14 Pa (G') after 30 min. ZnO nanoparticles (diameter 70 nm, +37 mV zeta-potential) at a concentration of 1 mg/mL prolonged CT to 20.8 ± 3.6 min and provoked a weak clot (CS 1.5 ± 1.0 Pa). However, at a lower concentration of 100 µg/mL the ZnO particles dramatically reduced CT to 6.0 ± 0.5 min and increased CS to 171 ± 63 Pa. This procoagulant effect decreased at lower concentrations reaching the detection limit at 10 ng/mL. SiO2 nanoparticles (diameter 232 nm, -28 mV zeta-potential) at high concentrations (1 mg/mL) reduced CT (2.1 ± 0.2 min) and stimulated CS (249 ± 59 Pa). Similar to ZnO particles, this procoagulant effect reached a detection limit at 10 ng/mL. Nanoparticles in high concentrations reproduce the surface charge effects on blood coagulation previously observed with large particles or solid metal oxides. However, nanoparticles with different surface charges equally well stimulate coagulation at lower concentrations. This stimulation may be an effect which is not directly related to the surface charge.
Estimation of Multiclass and Multilane Counts from Aggregate Loop Detector Data (2012)

Lane utilization on the highway is affected subtly by dynamic traffic management systems such as speed controls and lane management. To optimize the operation of dynamic traffic management, a better understanding of lane utilization is required, in particular, of how the flows of different vehicle classes (e.g., passenger cars, lorries) vary across the carriageway. Most loop detector systems do not collect this multilane, multiclass count data. This study developed a procedure for estimating multilane, multiclass counts from a variety of standard aggregate loop data formats from around the world. The estimation procedure involved the inference of multilinear regression laws that relate multilane, multiclass data to standard aggregate formats. The regression laws were then trained with small samples of individual vehicle data on a site-by-site basis. Preliminary results showed that the estimation procedure worked rather well, even when the input data were minimal—the extreme case being that of (U.S.-style) single-loop data, for which only flow and occupancy were available on a by lane basis. An error analysis indicated that small amounts of individual vehicle data were sufficient to train the estimator, provided they contained a representative mix of the flow behaviors at the site in question. Further work is required for the practical development of the tool, but it appears to have a wide range of potential uses for both researchers and practitioners.
Uric Acid Elimination in the Urine (2004)

Uric acid, a weak organic acid, has very low pH-dependent solubility in aqueous solutions. About 70% of urate elimination occurs in urine, the kidney standing as a major determinant of plasma levels. The complex renal handling results in a fractional clearance of less than 10%. Recently identified urate-specific transporter/channels are involved in tubular handling and extracellular transport. Extracellular fluid, rather than urine output, is the main regulator of urate excretion. A number of interfering agents, including widely used drugs such as aspirin, losartan, diuretics, may decrease or increase urate elimination. Hyperuricemia induced by hypouricosuria often accompanies the metabolic syndrome, and insulin resistance has been hypothesized as the common underlying defect. Hyperuricosuria, associated with dehydration or exercise, results in acute uric acid nephropathy, and causes an obstructive acute renal failure (ARF). This reversible ARF can be prevented by forced hydration with bicarbonate or saline solutions. Renal hypouricemia, due to mutations of urate transporter, is a rare cause of exercise-induced ARF. The existence of chronic urate nephropathy, gouty nephropathy, is still under discussion. Uric acid nephrolithiasis results from supersaturation, strongly influenced by low urine pH, rather than altered urate turnover. Alkali and fluid intake prove successful in managing uric acid stones.
The LatMix Summer Campaign: Submesoscale Stirring in the Upper Ocean (2015)

United States. Office of Naval Research (National Science Foundation (U.S.). Physical Oceanography Program Grant OCE-0751653)
Molecular properties of CD133+ glioblastoma stem cells derived from treatment-refractory recurrent brain tumors (2009)

Glioblastoma multiforme (GBM) remains refractory to conventional therapy. CD133+ GBM cells have been recently isolated and characterized as chemo-/radio-resistant tumor-initiating cells and are hypothesized to be responsible for post-treatment recurrence. In order to explore the molecular properties of tumorigenic CD133+ GBM cells that resist treatment, we isolated CD133+ GBM cells from tumors that are recurrent and have previously received chemo-/radio-therapy. We found that the purified CD133+ GBM cells sorted from the CD133+ GBM spheres express SOX2 and CD44 and are capable of clonal self-renewal and dividing to produce fast-growing CD133- progeny, which form the major cell population within GBM spheres. Intracranial injection of purified CD133+, not CD133- GBM daughter cells, can lead to the development of YKL-40+ infiltrating tumors that display hypervascularity and pseudopalisading necrosis-like features in mouse brain. The molecular profile of purified CD133+ GBM cells revealed characteristics of neuroectoderm-like cells, expressing both radial glial and neural crest cell developmental genes, and portraying a slow-growing, non-differentiated, polarized/migratory, astrogliogenic, and chondrogenic phenotype. These data suggest that at least a subset of treated and recurrent GBM tumors may be seeded by CD133+ GBM cells with neural and mesenchymal properties. The data also imply that CD133+ GBM cells may be clinically indolent/quiescent prior to undergoing proliferative cell division (PCD) to produce CD133- GBM effector progeny. Identifying intrinsic and extrinsic cues, which promote CD133+ GBM cell self-renewal and PCD to support ongoing tumor regeneration may highlight novel therapeutic strategies to greatly diminish the recurrence rate of GBM.
Anabolic Effects of 1,25-Dihydroxyvitamin D3 on Osteoblasts Are Enhanced by Vascular Endothelial Growth Factor Produced by Osteoblasts and by Growth Factors Produced by Endothelial Cells* (1997)

Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on HOB cocultured with endothelial cells. When HOB with high alkaline phosphatase (Al-P) activity and human umbilical vein endothelial cells (HUVEC) with little activity were cultured together, Al-P activity increased, accompanied by an increase in cell number. When HOB and HUVEC were cultured separately, 1,25-(OH)2D3 did not directly stimulate [3H]thymidine incorporation into HUVEC, but stimulated it in the presence of HOB. VEGF did not directly stimulate the Al-P activity of HOB but stimulated it in the presence of HUVEC. The conditioned medium of HOB stimulated the proliferation of HUVEC, and this was partially blocked by anti-VEGF antibody. Conversely, the conditioned medium of HUVEC increased Al-P activity and [3H]thymidine incorporation into HOB, and this was partially blocked by antiinsulin-like growth factor I antibody and BQ-123, a specific antagonist of the endothelin-1 (ET-1) receptor. 1,25-(OH)2D3 stimulated the release of VEGF and ET-1 from HOB and HUVEC, respectively. Furthermore, the 1,25-(OH)2D3-induced release of VEGF was enhanced in HOB cocultured with HUVEC. A quantitative reverse transcription-PCR study revealed that genes for VEGF receptors (Flt-1 and KDR) were expressed in HUVEC, but not in HOB, and that 1,25-(OH)2D3 increased the levels of expression of VEGF receptor genes in endothelial cells only when cocultured with HOB. In summary, we demonstrated that 1,25-(OH)2D3 exerts an anabolic effect on osteoblasts by enhancing their production of VEGF, which stimulates its receptors on endothelial cells, followed by increased production of osteotropic growth factors, such as insulin-like growth factor I and ET-1. These in vitro findings suggest that the VEGF/VEGF receptor system may be involved in both bone formation and bone remodeling in vivo.
Nucleophilic and acid catalysis in phosphoramidite alcoholysis (2001)

Numerous triflates, mesylates, chlorides, trifluoroacetates and tetrazolides of trialkylammonium, pyridinium and azolium ions have been studied as activators for the reaction of N,N,O,O′-tetraisopropylphosphoramidite with propan-2-ol in acetonitrile. The progress of the reactions was followed by 31P NMR spectroscopy, and the pKa values of the activators were determined by a 13C NMR spectroscopic method based on competing simultaneous protonation of two bases. The salts promoted the reaction both as acids and nucleophiles, the acidity playing a more important role than the nucleophilicity. The Brønsted α value for the general acid catalysis was observed to range from 0.6 to 0.9 and the βnucl value to be 0.2 (pKa of the conjugate acid used as the measure of nucleophilicity). Mixtures of neutral azoles or pyridines and weakly acidic ammonium salts were also shown to be useful activators that allow the acidity and nucleophilicity to be tuned independently of each other.
Some Investigations into the Dynamic Mass Transfer at the Slag–Metal Interface Using Sulfur: Concept of Interfacial Velocity (2011)

In the current work, dynamic studies of mass transfer of sulfur from the gas phase to the metal phase of pure iron through CaO-SiO2-Al2O3-FeO quaternary slag were carried out. X-ray videos were taken that were later processed to identify the oscillation of the metal drop occurring during the mass transfer. It was observed that the metal drop had hybrid oscillations. Each of these oscillations could be identified as composed of a symmetric and an asymmetric element, which was attributed to the changes in the shape of the droplet. The latter (asymmetric part) could be identified by the deviation of the left and right contact angles from the stable configuration. The symmetric oscillations were traced to the surface movement of sulfur at the interface, which created an instantaneous area change at the slag–metal interface. This area change was due to the combined effect of Marangoni flow and interface dilatation. The velocity of sulfur at the interface was calculated from the area change and had a maximum order of magnitude as 10−4 m/s. It was also observed that the interfacial velocity increased with increase in temperature.
Effects of lithium treatment on granulocytes and granulocyte stimulating factor in patients with bipolar affective disorder (2001)

EFFECTS OF LITHIUM TREATMENT ON GRANULOCYTES AND GRANULOCYTE COLONY-STIMULATING FACTOR IN PATIENTS WITH BIPOLAR AFFECTIVE DISORDER Objective: Although there are conflicting results, lithium carbonate has been demonstrated to induce the production of haematopoietic cells, particularly white blood cell series. In this study, we examined the effects of lithium on white blood cells in association with granulocyte colony-stimulating factor (G-CSF), which is a polypeptide growth factor that regulates the production of neutrophilic granulocytes. Methods: Eighteen lithiumnaive (8 females, 10 males; mean±SD age: 36±7.9 years) and 20 long-term lithium treated (9 females, 11 males; mean±SD age: 37.4±9.5 years) bipolar patients were included in the study. In the lithium-naive patients, lithium treatment was started to provide prophylactic serum lithium concentrations after blood samples were taken to determine the baseline haematological values (white blood cell, granulocyte and lymphocyte counts, haematocrit and G-CSF concentration). Blood samples were reobtained in the first and fourth weeks in this group. The same measurements were fulfilled once in the patients on the longterm lithium treatment. Results: The values of granulocyte count were significantly increased in the fourth week of lithium administration compared to the baseline values in the patients who were in the short-term lithium treatment, and this increase was associated with some elevation of G-CSF values that did not reach significance. The values of granulocyte count in the longterm lithium group were not significantly different from those of the baseline values of lithium-naive patients. Conclusion: Granulocytosis induced by lithium treatment in bipolar patients cannot be explained solely by the stimulation of G-CSF activity. Increased granulocyte count seems to approach the baseline values during the long-term lithium treatment.
Finite Element modeling of hexagonal surface acoustic wave biosensor based on LiTaO<inf>3</inf> (2008)

The main advantage of numerical methods such as Finite Elements (FE) in modeling surface acoustic wave (SAW) devices lies in their ability to model devices involving complicated transducer geometries. We present a 3-D finite element model of a novel hexagonal SAW biosensor based on LiTaO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> substrate. This SAW biosensor involves the use of one delay path for biological species detection whereas the other delay paths are used to simultaneously remove the non-specifically bound proteins using the acoustic streaming phenomenon, thus eliminating biofouling issues associated with other biosensors. Prior to this biosensor fabrication on any piezoelectric substrate, it is important to establish the type of waves that are generated along the various delay paths. The choice of a delay path for sensing and simultaneous cleaning application depends on the propagation characteristics of the wave generated along the crystal cut and orientation corresponding to that delay path. The frequency response as well as wave propagation characteristics along the delay path corresponding to crystal orientation with on-axis propagation along 36deg YX LiTaO3 substrate are analyzed using a coupled field FE model. Similar analysis is extended to the off-axis propagation directions corresponding to Euler rotations by 60deg and -60deg along the x-z plane. Our findings indicate that the on-axis direction with a significant surface shear horizontal (SH) component should be employed for biological species detection whereas the off-axis directions having mixed modes with a dominant Rayleigh wave component are most suitable for simultaneous cleaning or removal application.
Adjunctive Intraoperative Arterial Dilation (1981)

• A new balloon dilation catheter based on the concept of linear extrusion of the dilating element was developed and used clinically. The operative results were satisfactory with a substantial increase in lumen size that was found in more than 90% of the cases studied. Operative complications related to this technique were noted in two of 66 patients. A longer-term follow-up and increased application are required to determine the spectrum of use and durability of the procedure. (<i>Arch Surg</i>1981;116:1391-1398)
FMS-like tyrosine kinase 3 inhibitors: a patent review (2011)

Our understanding of Flt3 inhibitors has been increased by findings from recent preclinical and clinical trials. Some Flt3 inhibitors show good efficacy in AML patients, but relapse and resistance to these inhibitors are still unavoidable. To address these problems, structurally diverse inhibitors, which exhibit inhibitory activities against both wild type and mutated Flt3, should be explored.
Coverage List: a provider-patient database supporting advanced hospital information services. (1994)

We have developed a provider-patient database system, known as Coverage List, which maintains the associations between house staff and inpatients in a teaching hospital. Coverage List automatically links each patient to the proper resident when the patient is admitted, and updates the linkage whenever the resident coverage changes due to night or weekend coverage, physician illness, changes in clinical rotations, and other factors. Using this association, decision-support applications that detect significant clinical events can transmit them directly to the responsible resident. Sign-out and patient-review systems, which collect information on all of a physician's patients, always know the patients for whom that physician is responsible. Nurses who need to contact a physician about a patient issue always know which physician is covering that patient. Coverage List also manages schedule entry and display for physicians, or for any other staff members. A physician can enter individual schedule changes, sign out her service and her pager for the day, and page consultants automatically without going through an operator. These functions support clinical practice directly and enhance the value of other clinical programs.