gebhart/patrepeval huggingface dataset:
Real-Time Sensing of Single-Ligand Delivery with Nanoaperture-Integrated Microfluidic Devices (2017)

The measurement of biological events on the surface of live cells at the single-molecule level is complicated by several factors including high protein densities that are incompatible with single-molecule imaging, cellular autofluorescence, and protein mobility on the cell surface. Here, we fabricated a device composed of an array of nanoscale apertures coupled with a microfluidic delivery system to quantify single-ligand interactions with proteins on the cell surface. We cultured live cells directly on the device and isolated individual epidermal growth factor receptors (EGFRs) in the apertures while delivering fluorescently labeled epidermal growth factor. We observed single ligands binding to EGFRs, allowing us to quantify the ligand turnover in real time. These results demonstrate that this nanoaperture-coupled microfluidic device allows for the spatial isolation of individual membrane proteins while maintaining them in their cellular environment, providing the capability to monitor single-ligand binding events while maintaining receptors in their physiological environment. These methods should be applicable to a wide range of membrane proteins.
Concise routes to pyrazolo[1,5-a]pyridin-3-yl pyridazin-3-ones (2007)

Cycloaddition of pyridine N-imine with 6-alkyl-4-oxohex-5-ynoates followed by condensation with hydrazine provides concise access to pharmacologically active 6-(pyrazolo[1,5-a]pyridin-3-yl)pyridazinones. For the first time alkynyl heterocycles are also shown to be effective dipolarophiles for pyridine N-imine, and analogous compounds can be accessed directly in modest yields through the reaction of 6-(alkyn-1-yl)pyridazin-3-one derivatives.
Central nervous system regulation of gonadal development in the avian male (2000)

Many avian species, particularly domesticated ones used for egg and meat production, are photoperiodic. Research conducted over the past several years has revealed the neural components, neurotransmitters, neuromodulators, and gonadotropins that play an important role in responding to changes in day length. An ultimate effect of the neuroendocrine response of birds to light is gonadal development and production of fertilized eggs and young for the next generation. The main purpose of this paper is to address the major neural systems that have been shown to affect reproductive function in males. Potential areas of research that would help elucidate the mechanism of neural activation of gonadal function are suggested.
A New Convivencia (2019)

This chapter looks at Spanish administration of northern Morocco after the Rif War. As the physical border between Spain and Morocco disintegrated, Spanish colonial administrators looked for ways to promote “Hispano-Moroccan brotherhood” while preserving religious, social, and sexual boundaries between Moroccan Muslims, Jews, and Spanish settlers. While much scholarship in this area has been dedicated to exposing the Spanish colonial rhetoric of brotherhood to be a ruse, this chapter takes seriously the notion that the Spanish colonial administration attempted to distinguish itself from its French counterpart—even to the point of weakening the positions of the sovereign Spanish cities of Ceuta and Melilla. It aimed to demonstrate greater respect for local customs and traditions and to elevate the zone’s Muslim “caliph” to the status of sovereign, although in other ways its practices resembled the French model.
Studies on Ion Exchange Resins. XV. Selectivity Coefficients of Methacrylic Acid Resins toward Alkali Metal Cations (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Ion Exchange Resins. XV. Selectivity Coefficients of Methacrylic Acid Resins toward Alkali Metal CationsHarry P. Gregor, Mary Jane Hamilton, Ramesh J. Oza, and Fabian BernsteinCite this: J. Phys. Chem. 1956, 60, 3, 263–267Publication Date (Print):March 1, 1956Publication History Published online1 May 2002Published inissue 1 March 1956https://pubs.acs.org/doi/10.1021/j150537a002https://doi.org/10.1021/j150537a002research-articleACS PublicationsRequest reuse permissionsArticle Views641Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
High tumoral PD-L1 expression and low PD-1 + or CD8 + tumor-infiltrating lymphocytes are predictive of a poor prognosis in primary diffuse large B-cell lymphoma of the central nervous system (2019)

We investigated the clinicopathological role of the PD-1/PD-L1 pathway in primary diffuse large B-cell lymphoma of the central nervous system (PCNS-DLBCL) arising in the immune-privileged site. PD-L1 immunostaining of ≥30% of tumor cells was defined as tPD-L1+, and PD-L1 immunostaining of ≥30% of total cellularity, including tumor and non-tumoral cells, as tmPD-L1+ . PD-1<sup>+</sup> and CD8<sup>+</sup> tumor-infiltrating lymphocytes (TILs) were enumerated. Thirty-five cases (35.7%) were tPD-L1+ and 47 cases (48%) were tmPD-L1+ . The number of TILs was greater in tmPD-L1+ cases than in tmPD-L1- cases (CD8<sup>+</sup>, <i>P</i>= .050; PD-1<sup>+</sup>, <i>P</i>= .019). tPD-L1+ and tmPD-L1+ cases tended to have a poor performance status. In contrast, the numbers of CD8<sup>+</sup> and PD-1<sup>+</sup> TILs tended to be higher in patients with a good performance status and MYC/BCL2 negativity. Patients with tPD-L1+ had a worse overall survival (<i>P</i>= .026), and those with increased CD8<sup>+</sup> or PD-1<sup>+</sup> TILs tended to have a better overall survival (<i>P</i>= .081 and 0.044, respectively). Tumoral PD-L1 expression and the number of PD-1<sup>+</sup> TILs were independent prognostic factors. tPD-L1+ patients with a small number of CD8<sup>+</sup> or PD-1<sup>+</sup> TILs had the worst prognosis, and tPD-L1- patients with a large number of CD8<sup>+</sup> or PD-1<sup>+</sup> TILs had the best prognosis. In validation group, increased CD8<sup>+</sup> or PD-1<sup>+</sup> TILs were significantly associated with a prolonged survival, but PD-L1 had no prognostic significance. In conclusion, PD-L1 is frequently expressed in tumor cells and the immune microenvironment of PCNS-DLBCL and is correlated with increased TILs. PD-L1 and CD8<sup>+</sup> and PD-1<sup>+</sup> TILs have potential as prognostic biomarkers and therapeutic targets in PCNS-DLBCL.
Anorexic and Behavioural Effects of a New Imidazo-Isoindole Derivative (Mazindol) in Comparison with <i>d</i>-Amphetamine in the Rat (1977)

The effect of an imidazo-isoindole derivative (mazindol) upon motor and feeding activity and two different avoidance behaviours have been compared with those of d-amphetamine in rats. It was found that both drugs depress feeding activity in a dose-related manner and increase the motor activity of the animals. However, the ratio of the lowest motility-increasing dose and the lowest appetite suppressant dose is 0.5 for d-amphetamine and 2 for mazindol. Mazindol, like d-amphetamine, caused a stereotyped behaviour in rats when given in very high doses, but the range between the anorexic and the stereotyped behaviour dose is much higher for mazindol than for d-amphetamine. The shuttle-box avoidance behaviour suppressed by tetrabenazine is completely restored by d-amphetamine and partially by mazindol. Lever-pressing avoidance suppressed by tetrabenazine is restored by d-amphetamine while mazindol at the doses used is ineffective. It is concluded that mazindol could be an anorexic agent better suited than d-amphetamine for clinical use.
Current-Iteration Non-Causal Iterative Learning Control for Beam Loading Cancellation in Particle Accelerators (2019)

Iterative learning control (ILC) is an approach to improve the performance of a system that carries out the same operation repeatedly. In this work, ILC is used to reduce beam loading effect in cavity resonators. Since beam loading is a repeating disturbance, the error is repetitive as well, and the controller can use a non-causal learning function to preemptively counteract the beam loading effect. The learning filter parameters are chosen using simulations, then the controller is implemented on hardware to control the field of a normal conducting quarter wave cavity resonator.
Efficient one-pot synthesis of 2,3-dihydroquinazolin-4(1H)-ones from aromatic aldehydes and their one-pot oxidation to quinazolin-4(3H)-ones catalyzed by Bi(NO3)3·5H2O: Investigating the role of the catalyst (2011)

An efficient and novel synthesis of 2,3-disubstituted 2,3-dihydroquinazolin-4(1 H )-ones via one-pot, three-component reaction of isatoic anhydride, primary amines and aromatic aldehydes catalyzed by Bi(NO 3 ) 3 ·5H 2 O under solvent-free conditions is described. Oxidation of these 2,3-dihydroquinazolin-4(1 H )-ones to their quinazolin-4(3 H )-ones was also successfully performed in the presence of Bi(NO 3 ) 3 ·5H 2 O. This new method has the advantages of convenient manipulation, short reaction times, excellent yields, very easy work-up, and the use of commercially available, low cost and relatively non-toxic catalyst. The role of Bi(NO 3 ) 3 ·5H 2 O was also investigated in these transformations.
One-Shot Multigrid for the Optimal Neumann Boundary Control of Time-Periodic Partial Differential Equations (2009)

We present a one‐shot multigrid method for the optimal Neumann boundary control of time‐periodic, linear, parabolic partial differential equations. We focus on optimal control problems of the so‐called tracking type, i.e., we try to steer the PDE solution such that it matches a prescribed periodic trajectory, or segment thereof, as closely as possible. To that end we derive the optimality condition of such problems. We develop a one‐shot multigrid method for solving the resulting coupled system of forward and backward time‐periodic differential equations. Numerical examples are presented to illustrate the behavior of the multigrid algorithm.
Supplementary Figure Legends 1-6 from SLITs Suppress Tumor Growth <i>In vivo</i> by Silencing <i>Sdf1/Cxcr4</i> within Breast Epithelium (2023)

Supplementary Figure Legends 1-6 from SLITs Suppress Tumor Growth &lt;i&gt;In vivo&lt;/i&gt; by Silencing &lt;i&gt;Sdf1/Cxcr4&lt;/i&gt; within Breast Epithelium
Pulse method for measuring sound absorption in the kilohertz range (1976)

Solid-dielectric capacitance transducers, 25.4 cm in diameter, have been constructed and used to generate and detect pulses of sound waves in the frequency range from 4 to 200 kHz. The waves are propagated inside an aluminum tube, 25.4 cm i.d. and 4.5 m long. One of the transducers generates the sound pulses and can be moved axially along the tube; the second serves as a microphone, detecting the sound pulse and reflecting it at one end of the tube. The transducers are made by stretching metalized Mylar across a polished backing plate. Effects of diffraction, transducer nonalignment, and transverse modes within the tube are discussed. The sound absorption is obtained by the measurement of either the attenuation of the first pulse or the increase in the echo decay rate with distance. Pulse heights are measured with a multichannel pulse height analyzer and an on-line computer. The system has been used to measure sound absorption in humid air. [Work supported by NASA-Lewis Research Center.]
Destruction of hematopoietic microenvironment by cytotoxic T cells. (1997)

Coculture of cytotoxic T cells (STIL-3 C5) derived from L8313 leukemic mice with hematopoietic supportive stromal cells (MS-5) resulted in the detachment of MS-5 cells from the culture dish, whereas helper T cells (STIL-3 DF) did not induce this detachment. The response of bone marrow (BM) adherent cells to the same treatment was similar to that of MS-5 cells. The detached cells were unable to proliferate further, and genomic DNA of these cells showed fragmentation, suggesting that hematopoietic stromal cells died of apoptosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that STIL-3 C5 cells, but not STIL-3 DF cells expressed perforin, granzyme A & B, and Fas ligand. Fas was expressed in MS-5, BM adherent cells, MS-K and NIH/3T3 cells, which do not support hematopoiesis. These data suggest that the aforementioned factors mediate induction of apoptosis in MS-5 cells induced by direct cell-to-cell interaction with STIL-3 C5. This may explain the mechanism responsible for the destruction of the hematopoietic microenvironment by cytotoxic T cells in L8313 leukemia, from which STIL-3 cells are derived; it also suggests that destruction of hematopoietic tissue may be caused by leukemic cytotoxic T cells in some cases of leukemia.
Highly Increased Flow-Induced Power Generation on Plasmonically Carbonized Single-Walled Carbon Nanotube (2016)

We generate networks and carbonization between individualized single-walled carbon nanotubes (SWCNTs) by an optimized plasmonic heating process using a halogen lamp to improve electrical properties for flow-induced energy harvesting. These properties were characterized by Raman spectra, a field-emission-scanning probe, transmission electron microscopy, atomic force microscopy and thermographic camera. The electrical sheet resistance of carbonized SWCNTs was decreased to 2.71 kΩ/□, 2.5 times smaller than normal-SWCNTs. We demonstrated flow-induced voltage generation on SWCNTs at various ion concentrations of NaCl. The generated voltage and current for the carbonized-SWCNTs were 9.5 and 23.5 times larger than for the normal-SWCNTs, respectively, based on the electron dragging mechanism.
Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells (2009)

Human embryonic stem cells (hESCs) are pluripotent stem cells derived from the inner cell mass of human blastocysts. hESCs have become a great asset to studying human diseases and genetic functions of healthy organisms. The rate at which hESCs are being used in laboratories is exponentially increasing, and with that, the need for xeno-free hESCs is also increasing. Xeno-free grade hESCs, cells that have not come into contact with any animal-derived components except those of human origin, are critical for eventual drug therapy, cell therapy, and disease treatment in humans. However, advances toward a xeno-free hESC environment are still being developed. Replacement of murine feeder layers with extracellular matrix proteins has advanced the research, and some advances toward a serum-free and feeder-free environment for hESCs are described in this chapter.
Effect of doppler spread on OFDM based UWB systems (2005)

We investigate the inter-carrier interference (ICI) caused by relative motion between transmitter and receiver in orthogonal frequency division multiplexing (OFDM) based ultra wide-band (UWB) communications. Since the Doppler shift is proportional to the subcarrier frequency, the time-selective fading is subcarrier dependent. An exact expression as well as bounds are derived for the degradation in performance, as measured by signal-to-interference ratio (SIR). A design example is given to illustrate the concepts developed In the work.
Hematopoietic stem cells in co-culture with mesenchymal stromal cells - modeling the niche compartments in vitro (2010)

Our data suggest that the mesenchymal stromal cell surface is the predominant site of proliferation of hematopoietic stem cells, whereas the compartment beneath the mesenchymal stromal cell layer seems to mimic the stem cell niche for more immature cells. The SDF-1/CXCR4 interaction and integrin-mediated cell adhesion play important roles in the distribution of hematopoietic stem cells in the co-culture system.
Self-Sustained Autonomous Wireless Sensor Network with Integrated Solar Photovoltaic System for Internet of Smart Home-Building (IoSHB) Applications (2021)

Conventional wireless sensor networks (WSNs) in smart home-building (SHB) are typically driven by batteries, limiting their lifespan and the maximum number of deployable units. To satisfy the energy demand for the next generation of SHB which can interconnect WSNs to make the internet of smart home-building (IoSHB), this study introduces the design and implementation of a 250 mW to 2.3 W energy harvesting device. The proposed device is dynamically autonomous owing to the integration of embedded solar photovoltaic (PV) modules and power storage through a supercapacitor (SC; 5 V, 0.47 F) capable of powering WSNs for 95 s (up to 4.11 V). The deployed device can harvest indoor and outdoor ambient light at a minimum illumination of 50 lux and a maximum illumination of 200 lux. Moreover, the proposed system supports wireless fidelity (Wi-Fi) and Bluetooth Low Energy (BLE) to do data transfer to a webserver as a complete internet of things (IoT) device. A customized android dashboard is further developed for data monitoring on a smartphone. All in all, this self-powered WSN node can interface with the users of the SHBs for displaying ambient data, which demonstrates its promising applicability and stability.
Predicting Reactivity of Feedstocks to Resid Hydroprocessing Using Asphaltene Characteristics (2013)

Keeping all experimental conditions constant, feed reactivity is found to depend upon feed origin, composition, previous history, level of non-indigenous contaminants, etc. In the first part of this work, the preparative separation and characterization of the asphaltenes from seven resids of different origins and types were carried out to correlate asphaltene characteristics with feed reactivity to hydroprocessing in the presence of a Ni/Mo-dispersed catalyst (418 °C, 2.2 wt % molybdenum catalyst and 0.2 wt % nickel catalyst, and pressurized to 18.3 MPa of hydrogen for 2 h). Feed reactivity was measured in terms of the hydrodenitrogenation pseudo-first-order rate constant (HDN rate in h–1) and the percentage of reduction of microcarbon residue (% MCR). The results indicated that the hydroprocess reactivity of a given resid can be predicted reasonably well (R2 = 0.90–0.93 range) using the concentration of asphaltenes, their aromaticity, and degree of aromatic ring condensation. In the second part, the work was focused on directly determining the asphaltene solubility characteristics of the resids and correlated them to resid reactivity to hydroprocessing. Two recently developed methods were employed: asphaltene solubility fraction and asphaltene solubility profile. The results showed that the hydroprocess reactivity can be effectively predicted with correlation factors in the 0.93–0.94 range. These results indicate that feed reactivity and asphaltene solubility are linked. Therefore, these asphaltene characterization methods can be used to estimate resid reactivity without the need of time-consuming asphaltene separation and characterization.
Surface bound bisphosphonate for implant fixation in bone (2008)

During the surgical preparation of bone, prior to insertion of an implant, bone will be traumatized which leads to local resorption. Consequently, early implant fixation might be reduced. Impaired early fixation, as evidenced by radiostereometry, has been associated with increased risk of late loosening. Bisphosphonates are known to inhibit bone resorption by osteoclasts and have shown to increase implant fixation when administered systemically or locally directly at the bone prior to implant insertion. A method to bind bisphosphonates directly to the implant was developed. Stainless steel screws were coated with crosslinked fibrinogen, serving as an anchor for bisphosphonate attachment. The screws were inserted in the tibial metaphysis in rats and implant fixation was analyzed with pullout measurements. Bisphosphonate coated screws turned out to have 28 % higher pullout force at 2 weeks compared to control screws with the fibrinogen coating only. The next experiment was designed to measure at what stage in the healing process the strongest bisphosphonate effect was gained. Bisphosphonate coated screws were expected to reduce the resorption of the traumatized bone. However, no decreased fixation was found in the control group. Instead, the fixation increased with time, and so did the effect of the bisphosphonates. At 8 weeks, the pullout force was twice as high for screws with bisphosphonate compared to control screws. By histology at 8 weeks, a bone envelope was found around bisphosphonate coated screws but absent around control screws. Thus, the anti catabolic action of the bisphosphonate resulted in an increased amount of bone surrounding the bisphosphonate screws. Titanium is generally considered to be better fixated in bone compared to stainless steel. The coating technique was found to be applicable on titanium as well, again with improved fixation. A majority of fractures occur in osteoporotic bone. Despite the relatively low amount of bisphosphonates at the screws, the bisphosphonate coating improved implant fixation at 2 weeks also in rats made osteoporotic by ovariectomy. In conclusion, bisphosphonates bound to titanium or stainless steel screws coated with fibrinogen increased fixation in bone, in rats. These results suggest that the bisphosphonate and fibrinogen coating might improve the fixation of screw shaped implants and possibly also arthroplasties, in humans.
The Problem of Red Cell Polyagglutinability (1972)

Familiarity with the phenomenon of red cell polyagglutinability, while of prime importance to laboratory personnel, can be useful to the clinician as well. The presence of T-polyagglutinability, for instance, may be a clue to infection. Thrombocytopenia, leukopenia, or even hemolytic anemia can be associated with Tn-polyagglutinability. The Cad type of polyagglutinability is inherited.
Brewing with Rice Malt - A Gluten-free Alternative (2010)

The preparation of beer-like beverages with rice malt as the only raw material is reported. Several tests were performed on a laboratory scale and in a 25 L-capacity pilot plant. Both the decoction and the infusion procedure were tested; malt and water were mixed in a ratio 1:3.5 for both methods and the mash was brewed without adding exogenous enzymes. The obtained worts were fermented using bottom fermenting yeasts, while “beers” were re-fermented utilizing top fermenting yeasts and adding either sterile wort or sugar. A maximum ethanol of 4.5% vol. was obtained after the primary fermentation from an initial wort with an original gravity of 11.8°Plato. All parameters of the beer were found to be acceptable using a standard beer analysis. Owing to a suitable hop addition, an aroma very similar to that of a normal beer was obtained.