An Improved Protein Bioreactor (2002)

In vitro protein biosynthesis became a powerful technology for biochemical research. Beside the determination of structure and function in vitro selection of proteins is also of great interest. In most cases the use of a synthesized protein for further applications depends on its purity. For this purpose the in vitro production and purification of proteins with short affinity tails was established. A cell-free protein synthesis system was employed to produce bovine heart fatty acid-binding protein and bacterial chloramphenicol acetyltransferase with and without fusion of the Strep-tag affinity peptide. The quantitative removal of fusion protein during cell-free synthesis from a batch reaction and a semicontinuous flow cell-free reactor were achieved. No significant influence of the Strep-tag and the conditions during the affinity chromatography on maturation or of the proteins were The removal from the flow cell-free reactor is the use of The to affinity In vitro protein biosynthesis became a powerful technology for biochemical research. Beside the determination of structure and function in vitro selection of proteins is also of great interest. In most cases the use of a synthesized protein for further applications depends on its purity. For this purpose the in vitro production and purification of proteins with short affinity tails was established. A cell-free protein synthesis system was employed to produce bovine heart fatty acid-binding protein and bacterial chloramphenicol acetyltransferase with and without fusion of the Strep-tag affinity peptide. The quantitative removal of fusion protein during cell-free synthesis from a batch reaction and a semicontinuous flow cell-free reactor were achieved. No significant influence of the Strep-tag and the conditions during the affinity chromatography on maturation or of the proteins were The removal from the flow cell-free reactor is the use of The to affinity The of the in vitro protein biosynthesis system the production of proteins also the synthesis of or proteins in The of the in vitro protein biosynthesis the the of and the of in in and proteins cell-free The for the of the or for and proteins or of cell-free protein synthesis from or For the production in a the flow fatty acid-binding protein from bovine chloramphenicol semicontinuous flow flow fatty acid-binding protein from bovine chloramphenicol semicontinuous flow The of the in vitro protein biosynthesis A cell-free system of in and A semicontinuous system a to of a batch The removal from the reactor is The of the the proteins or with the In during the reaction the use of a synthesized protein for further applications or depends to a great its purity. 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