gebhart/patrepeval huggingface dataset:
Tissue factor expression in human leukemic cells (1996)

Patients with acute leukemia are at increased risk for thrombotic and hemorrhagic complications, particularly those patients with acute promyelocytic leukemia (APL) undergoing induction chemotherapy. These serious complications have been attributed by some authors to the release of tissue factor (TF) procoagulant activity (PCA), particularly during cytotoxic chemotherapy. In previous studies of normal peripheral blood cells, only cells of the monocyte lineage have been found to express TF PCA. Therefore, several questions remain regarding the origin and characterization of the PCA in malignant leukemic cells, particularly those thought to be derived from granulocyte progenitor cells. We utilized a full-length cDNA probe, several monoclonal antibodies (MAbs) and a sensitive one-stage PCA assay to study the expression of TF in the human cell line, HL-60, in human peripheral blood monocytes/macrophages (Mo/Mø) and in highly purified populations of human polymorphonuclear leukocytes (PMN). In the HL-60 cells we detected low but significant levels of TF mRNA and TF antigen (TF:Ag). In unstimulated cells, coordinate increased levels of TF mRNA, TF:Ag and TF PCA expression were noted following phorbol-ester-induced macrophage differentiation of the cells, but a decreased level of TF mRNA with no change in the basal level of TF:Ag expression occurred following retinoic acid-induced granulocyte differentiation of this cell line. Long-term cultures of stimulated mature Mo/Mø demonstrated initial coordinate expression of TF mRNA, TF:Ag and TF PCA, but TF:Ag expression persisted even after 7 days (when TF PCA was undetectable). No TF PCA, TF:Ag or TF mRNA was demonstrated in highly purified populations of human PMN, regardless of culture conditions. Discordant expression of TF mRNA, TF:Ag and TF PCA in HL-60 cells suggests the possibility of novel, post-synthetic mechanisms for the regulation of TF PCA expression, which might be dependent on the phenotypic differentiation level of the cell. Such mechanisms (yet to be defined) might account for the ability of some leukemic cells, which frequently express characteristics of more than one cell line (e.g. monocytes and granulocytes), to express a TF gene product capable of activating blood coagulation.
Trajectory Tracking by Multi-Agent in Octagonal Formation using Conventional and Adaptive Control (2018)

Trajectory tracking by multi-agent in the octagonal formation using conventional and adaptive Control is proposed in this paper. To achieve synchronization of octagonal formation and tracing leaders trajectory, adaptive Proportional (P) controller and the adaptive controller is used. For rescue operation in military and agricultural field applications, it is necessary to analyze the behavior of agents information for various control inputs. The connectivity of agents information is maintained using neighborhood information, observer and navigational feedback control strategy. The use of P controller strategy results in lesser average position error and more settling time when compared to the adaptive controller. The adaptive tuning of P controller and adaptive controller plays an important role in analyzing the behavior of agents in the octagonal formation and to perform flocking along the leader's trajectory.
Anatomische Gesichtspunkte zur harten Hirnhaut (2010)

Das Still'sche Motto, dass Osteopathie Anatomie, Anatomie, Anatomie sein soll, hat Generationen von Osteopathen inspiriert, beantwortet jedoch nicht die alles entscheidende Frage: Welche Anatomie brauchen wir für unser Wahrnehmen und Handeln? Der Beitrag beschreibt die aus osteopathischer Sicht relevanten anatomischen Aspekte der Dura mater.
PPARβ Interprets a Chromatin Signature of Pluripotency to Promote Embryonic Differentiation at Gastrulation (2013)

Epigenetic post-transcriptional modifications of histone tails are thought to help in coordinating gene expression during development. An epigenetic signature is set in pluripotent cells and interpreted later at the onset of differentiation. In pluripotent cells, epigenetic marks normally associated with active genes (H3K4me3) and with silent genes (H3K27me3) atypically co-occupy chromatin regions surrounding the promoters of important developmental genes. However, it is unclear how these epigenetic marks are recognized when cell differentiation starts and what precise role they play. Here, we report the essential role of the nuclear receptor peroxisome proliferator-activated receptor β (PPARβ, NR1C2) in Xenopus laevis early development. By combining loss-of-function approaches, large throughput transcript expression analysis by the mean of RNA-seq and intensive chromatin immunoprecipitation experiments, we unveil an important cooperation between epigenetic marks and PPARβ. During Xenopus laevis gastrulation PPARβ recognizes H3K27me3 marks that have been deposited earlier at the pluripotent stage to activate early differentiation genes. Thus, PPARβis the first identified transcription factor that interprets an epigenetic signature of pluripotency, in vivo, during embryonic development. This work paves the way for a better mechanistic understanding of how the activation of hundreds of genes is coordinated during early development.
Can Mesenchymal Stem Cells Induce Tolerance to Cotransplanted Human Embryonic Stem Cells? (2008)

Mesenchymal stem cells (MSCs) are reported to be immune privileged. We assessed whether their transplantation (Tx) could create a suppressive microenvironment mitigating rejection of coinjected human embryonic stem cells (hESCs). Three weeks after ligation-induced myocardial infarction, 40 immunocompetent rats received 150 microl of cardiac-specified hESCs (5 x 10(6)), MSCs (5 x 10(6)), hESC + MSC (5 x 10(6) for each), or control medium. Two months after Tx, left ventricle (LV) function was assessed by echocardiography, and hearts were processed for the detection of human cells by immunostaining and quantitative RT-PCR, patterns of rejection, fibrosis, and angiogenesis. Two months after Tx, LV ejection fraction (LVEF) was significantly higher in the ESC and ESC + MSC groups compared with controls. There were few engrafted cells, which expressed markers of endothelial, smooth muscle, and ventricular cardiac cells, particularly in the MSC group. Hearts of all groups demonstrated a similar infiltration by CD4(+) and CD3(+) cells but MSC-Tx resulted in a greater infiltration of FoxP3 compared with the control and ESC-alone groups. No teratoma was observed. Thus, cotransplantation of ESCs and MSCs provided better functional preservation compared with single-cell treatment alone. However, there was only modest evidence for an immunosuppressive effect of coinjected MSCs and their beneficial effects seemed rather mediated by trophic effects on the host tissue.
Participation in Daily Life of Children with Asthma (2008)

Asthma can have a negative effect on psychological and social well-being in childhood. Sports participation, school attendance, and quality of life are important issues for children with asthma and their parents. However, a structural evaluation of these factors is not always incorporated in the routine medical approach of children with asthma. Moreover, goals in asthma treatment, such as minimal symptoms and normal activity levels, are achieved in a minority of children. This review describes determinants that are important for the well-being of children with asthma and their parents. Besides the control of symptoms, factors such as sports participation, socializing in peer groups, school attendance, and quality of life must be considered. These issues are relevant when evaluating the management of children and adolescents with asthma. A multidisciplinary evaluation by a pediatrician, school nurse, gym teacher, and psychologist might contribute to an important decrease in the impact of asthma on daily life.
Storage of digital camera images (2003)

We describe a simple technique to improve the quality of images that are compressed for storage on a digital camera. We assume that the camera is designed to store a fixed number of images, and that lossy rate controlled compression is used to ensure that each image fits in the space allocated to it. Whenever the user takes fewer than the maximum allowed number of images a portion of the disk is empty, which is wasteful. Ideally we would like to make full use of the storage space, and not leave any empty. We show that when an image is JPEG compressed it is possible to produce a secondary compressed version for marginal additional computation. At the decoder the secondary version allows decoding of the image with much greater fidelity than is possible using the primary alone. Thus if the user takes half or fewer of the maximum allowed number of images, each will be reconstructed with far greater fidelity, and some images will always have enhanced quality provided that the disk does not fill.
Tumor-expressed factor VII is associated with survival and regulates tumor progression in breast cancer (2023)

Cancer enhances the risk of venous thromboembolism, but a hypercoagulant microenvironment also promotes cancer progression. Although anticoagulants have been suggested as a potential anticancer treatment, clinical studies on the effect of such modalities on cancer progression have not yet been successful for unknown reasons. In normal physiology, complex formation between the subendothelial-expressed tissue factor (TF) and the blood-borne liver-derived factor VII (FVII) results in induction of the extrinsic coagulation cascade and intracellular signaling via protease-activated receptors (PARs). In cancer, TF is overexpressed and linked to poor prognosis. Here, we report that increased levels of FVII are also observed in breast cancer specimens and are associated with tumor progression and metastasis to the liver. In breast cancer cell lines, tumor-expressed FVII drives changes reminiscent of epithelial-to-mesenchymal transition (EMT), tumor cell invasion, and expression of the prometastatic genes, SNAI2 and SOX9. In vivo, tumor-expressed FVII enhanced tumor growth and liver metastasis. Surprisingly, liver-derived FVII appeared to inhibit metastasis. Finally, tumor-expressed FVII-induced prometastatic gene expression independent of TF but required a functional endothelial protein C receptor, whereas recombinant activated FVII acting via the canonical TF:PAR2 pathway inhibited prometastatic gene expression. Here, we propose that tumor-expressed FVII and liver-derived FVII have opposing effects on EMT and metastasis.
Phytosterols, Cholesterol Absorption and Healthy Diets (2007)

The purpose of this review is to outline the emerging role of dietary phytosterols in human health. Dietary saturated fat, cholesterol and fiber are currently emphasized in the reduction of low-density lipoprotein cholesterol levels. However, other dietary components such as phytosterols may have equivalent or even larger effects on circulating cholesterol and need further study with respect to the potential for coronary heart disease risk reduction. Phytosterol effects were not considered in classic fat-exchange clinical trials and may account for some of the differences attributed to the food fats studied. Phytosterols reduce cholesterol absorption while being poorly absorbed themselves and the effects can be studied in human subjects in single-meal tests using stable isotopic tracers. Because phytosterols are insoluble and biologically inactive when purified, careful attention needs to be given to ensuring that commercial supplement products are rendered bioavailable by dissolution in fat or by emulsification. Recent work shows that phytosterols in natural food matrices are also bioactive. The retention of phytosterols during food manufacturing and the use of foods with high phytosterol content may constitute an alternative to the use of supplements.
Minimum requirements for laser-induced symmetry breaking in quantum and classical mechanics (2008)

Necessary conditions for generating phase controllable asymmetry in spatially symmetric systems using lasers are identified and are shown to be identical in quantum and classical mechanics. First, by studying the exact dynamics of harmonic systems in the presence of an arbitrary radiation field, it is demonstrated that anharmonicities in the system's potential are a necessary requirement for phase controllability. Then, by analyzing the space-time symmetries of the laser-driven Liouville dynamics for classical and quantum systems, a common set of temporal symmetries for the driving field that need to be violated to induce transport are identified. The conditions apply to continuous wave lasers and to symmetry breaking effects that do not rely on the control of the absolute phase of the field. Known examples of laser fields that can induce transport in symmetric systems are seen to be particular cases of these symmetry constraints.
Contribution of Gene Expression to Metabolic Fluxes in Hypermetabolic Livers Induced Through Burn Injury and Polymicrobial Sepsis in Rats (2006)

Severe injury activates many stress-related and inflammatory pathways leading to a systemic inflammatory and hypermetabolic state. Prior studies on livers isolated from hypermetabolic animal models have identified intrinsic metabolic flux changes that were not dependent upon the continual presence of elevated stress hormones and substrate loads. We investigated whether such changes may be due to more persistent alterations in gene expression and enzyme protein levels. We induced a systemic hypermetabolic response in rats by applying, under general anesthesia, a full-thickness burn injury covering ∼20% of the total surface body area followed two days later by cecum ligation and puncture (CLP) to induce polymicrobial sepsis. Controls consisted of animals receiving a sham-burn followed by CLP, or a sham-burn followed by sham-CLP. Two days after CLP, livers were removed and analyzed for gene expression profiling using DNA microarrays and for metabolism by ex vivo perfusion coupled with metabolic flux analysis (MFA). Burn...
Laser stroboscopic photography. Technique for cell orientation studies in flow. (1976)

Squamous cells in fluid flow are photographed using a laser stroboscopic technique. The technique provides either dark field or phase contrast multiple images of a cell on a single frame of film at resolutions permitted by the thickness of the cell stream. Cell orientation, velocity and acceleration are obtained from these photographs for evaluation of flow system performance.
Mitomycin-treated undifferentiated embryonic stem cells as a safe and effective therapeutic strategy in a mouse model of Parkinsonâ€™s disease (2015)

Parkinson's disease (PD) is an incurable progressive neurodegenerative disorder. Clinical presentation of PD stems largely from the loss of dopaminergic neurons in the nigrostriatal dopaminergic pathway, motivating experimental strategies of replacement based on cell therapy. Transplantation of dopaminergic neurons derived from embryonic stem cells significantly improves motor functions in rodent and non-human primate models of PD. However, protocols to generate dopaminergic neurons from embryonic stem cells generally meet with low efficacy and high risk of teratoma formation upon transplantation. To address these issues, we have pre-treated undifferentiated mouse embryonic stem cells (mESCs) with the DNA alkylating agent mitomycin C (MMC) before transplantation. MMC treatment of cultures prevented tumorigenesis in a 12 week follow-up after mESCs were injected in nude mice. In 6-OH-dopamine-lesioned mice, intrastriatal injection of MMC-treated mESCs markedly improved motor function without tumor formation for as long as 15 months. Furthermore, we show that halting mitotic activity of undifferentiated mESCs induces a four-fold increase in dopamine release following in vitro differentiation. Our findings indicate that treating mESCs with MMC prior to intrastriatal transplant is an effective to strategy that could be further investigated as a novel alternative for treatment of PD.
Unsupervised Parse Selection for HPSG (2010)

Parser disambiguation with precision grammars generally takes place via statistical ranking of the parse yield of the grammar using a supervised parse selection model. In the standard process, the parse selection model is trained over a hand-disambiguated treebank, meaning that without a significant investment of effort to produce the treebank, parse selection is not possible. Furthermore, as treebanking is generally streamlined with parse selection models, creating the initial treebank without a model requires more resources than subsequent treebanks. In this work, we show that, by taking advantage of the constrained nature of these HPSG grammars, we can learn a discriminative parse selection model from raw text in a purely unsupervised fashion. This allows us to bootstrap the treebanking process and provide better parsers faster, and with less resources. 1
Dimensionnement multimodèle pour la conception optimale des machines électriques. Méthodologie de conception optimale (2008)

'. L'evolution des moyens de calcul permet le deploiement de nouvelles demarches de conception, dont un exemple est presente dans cet article. Un modele multiphysique simple est construit pour le dimensionnement d'un moteur synchrone a aimants. La demarche consiste a associer a chaque phenomene physique le modele le plus approprie. Un modele elements finis est utilise pour representer le comportement magnetique de la machine et un modele analytique est utilise pour decrire les phenomenes thermiques. Ces modeles sont couples au sein d'un unique processus d'optimisation non lineaire sous contraintes et constituent ainsi un multimodele de dimensionnement. Cet exemple est representatif de la demarche de conception qui fait intervenir des activites complexes gerees par l'acteur humain et des activites compliquees a la charge d'un systeme formel theorique et pratique, et demontre qu'il est possible d'elaborer une vision globale et structuree de la demarche conception.
Impact of Valency of a Glycoprotein B-Specific Monoclonal Antibody on Neutralization of Herpes Simplex Virus (2010)

Herpes simplex virus (HSV) glycoprotein B (gB) is an integral part of the multicomponent fusion system required for virus entry and cell-cell fusion. Here we investigated the mechanism of viral neutralization by the monoclonal antibody (MAb) 2c, which specifically recognizes the gB of HSV type 1 (HSV-1) and HSV-2. Binding of MAb 2c to a type-common discontinuous epitope of gB resulted in highly efficient neutralization of HSV at the postbinding/prefusion stage and completely abrogated the viral cell-to-cell spread in vitro. Mapping of the antigenic site recognized by MAb 2c to the recently solved crystal structure of the HSV-1 gB ectodomain revealed that its discontinuous epitope is only partially accessible within the observed multidomain trimer conformation of gB, likely representing its postfusion conformation. To investigate how MAb 2c may interact with gB during membrane fusion, we characterized the properties of monovalent (Fab and scFv) and bivalent [IgG and F(ab')(2)] derivatives of MAb 2c. Our data show that the neutralization capacity of MAb 2c is dependent on cross-linkage of gB trimers. As a result, only bivalent derivatives of MAb 2c exhibited high neutralizing activity in vitro. Notably, bivalent MAb 2c not only was capable of preventing mucocutaneous disease in severely immunodeficient NOD/SCID mice upon vaginal HSV-1 challenge but also protected animals even with neuronal HSV infection. We also report for the first time that an anti-gB specific monoclonal antibody prevents HSV-1-induced encephalitis entirely independently from complement activation, antibody-dependent cellular cytotoxicity, and cellular immunity. This indicates the potential for further development of MAb 2c as an anti-HSV drug.
Pomegranate extract inhibits the interleukin-1β-induced activation of MKK-3, p38α-MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes (2010)

Our results provide an important insight into the molecular basis of the reported cartilage protective and arthritis inhibitory effects of pomegranate extract. These novel pharmacological actions of PE on IL-1β stimulated human OA chondrocytes impart a new suggestion that PE or PE-derived compounds may be developed as MKK and p38-MAPK inhibitors for the treatment of OA and other degenerative/inflammatory diseases.
Computational search for UV radiation resistance strategies in Deinococcus swuensis isolated from Paramo ecosystems (2019)

Ultraviolet radiation (UVR) is widely known as deleterious for many organisms since it can cause damage to biomolecules either directly or indirectly via the formation of reactive oxygen species. The goal of this study was to analyze the capacity of high-mountain Espeletia hartwegiana plant phyllosphere microorganisms to survive UVR and to identify genes related to resistance strategies. A strain of Deinococcus swuensis showed a high survival rate of up to 60% after UVR treatment at 800J/m2 and was used for differential expression analysis using RNA-seq after exposing cells to 400J/m2 of UVR (with >95% survival rate). Differentially expressed genes were identified using the R-Bioconductor package NOISeq and compared with other reported resistance strategies reported for this genus. Genes identified as being overexpressed included transcriptional regulators and genes involved in protection against damage by UVR. Non-coding (nc)RNAs were also differentially expressed, some of which have not been previously implicated. This study characterized the immediate radiation response of D. swuensis and indicates the involvement of ncRNAs in the adaptation to extreme environmental conditions.
Selective Recovery of β-Galactosidase With Charged Fusion Tails Using Ion-Exchange Membranes (1994)

We explored the feasibility of attaching charged tails to a target protein, in this case β-galactosidase, for selective recovery. In this process, an ion-exchange membrane was used for selective binding and release of β-galactosidase with the attached purification fusions. Strength of binding and purity of eluate increased with increasing tail length. In addition, activity yield was improved with the implementation of an intermediate partial elution recycle procedure.
Ecological momentary assessment of adolescent smoking cessation: A feasibility study (2008)

Attempts to quit smoking by adolescents typically fail, even when aided by psychosocial and pharmacological treatments. Gaining a better understanding of the process of smoking cessation and relapse in this population could lead to improved treatments and increases in cessation rates. Ecological momentary assessment (EMA) has been used to describe the relapse process among adults, but not among adolescents. This study examined the feasibility of using EMA to examine relapse among adolescent smokers. Participants (N = 13) used a hand-held computer for 3 weeks to report on their smoking behavior, affect state, and exposure to smoking cues during a quit attempt (7 days prequit, 14 days postquit). All of the participants recorded a quit attempt and at least one lapse during the monitoring interval. Compliance with the protocol was generally high but decreased slightly over time. As with adults, evidence indicated that lapses were associated with craving, negative affect, and smoking cues. These data support the feasibility and potential value of using EMA with adolescent smokers.