gebhart/patrepeval huggingface dataset:
OceanStore (2000)

OceanStore is a utility infrastructure designed to span the globe and provide continuous access to persistent information. Since this infrastructure is comprised of untrusted servers, data is protected through redundancy and cryptographic techniques. To improve performance, data is allowed to be cached anywhere, anytime. Additionally, monitoring of usage patterns allows adaptation to regional outages and denial of service attacks; monitoring also enhances performance through pro-active movement of data. A prototype implementation is currently under development.
Development of a Mathematical Model for Analyzing Oscillometoric Signals (2003)

In the blood pressure measurement, there are generally two methods. One is the auscultatory method which is widely used as a standard blood pressure measurement method. The other is the automatic sphygmomanometer. It is known that there is discrepancy in the measuring results of both methods. In the automatic sphygmomanometer, the measuring blood pressure is determined on the basis of the pressure of changes in the air pressure in a cuff. However, the relation between the blood pressure and the cuff air pressure has not been clarified. In this research, single degree of freedom model was applied to analyzing for development of the mathematical model which simulates the relation between the blood and the cuff pressure. As a result of the research, parameters, which show the relation between the blood pressure and the cuff pressure in the model, were able to be calculated.
Designing Novel Applications Inspired by Emerging Media Technologies (2011)

The field of Human-Computer Interaction provides a number of useful tools and methods for obtaining information on end-users and their usage context to inform the design of computer systems, yet relatively little is known on how to go about designing for a completely novel application where there is no user base, no existing practice of use available at the start. The success of the currently available HCI methodology that focuses on understanding users’ needs and establishing requirements is well-deserved in making computing applications usable in terms of fitting them to end-users’ usage contexts. However, too much emphasis on identifying user needs tends to stifle other more exploratory design activities where new types of applications are invented in order to discover or create new activities currently not practiced. In this paper, we argue that a great starting point of novel application design is not the problem space (trying to rigorously define the user requirements) but the solution space (trying to leverage emerging computational technologies and growing design knowledge for various interaction platforms), and we build a foundation for a pragmatic design methodology supported by the authors’ extensive experience in designing novel applications inspired by emerging media technologies.
Design and Performance of Frequency Selective Surface With Integrated Photodiodes for Photonic Calibration of Phased Array Antennas (2010)

The design, fabrication, and integration of a frequency selective surface (FSS) with integrated photodiodes to allow for photonic calibration of phased array antennas is presented. The design includes embedding electrically short dipole antennas in each unit cell of the FSS, with a zero-biased photodiode placed across the gap of the diode. Fibers from an optical distribution network are passed through the honeycomb core of the frequency selective surface and pigtailed to the photodiodes. The RF performance of the frequency selective surface with integrated optics is investigated via simulations and measurements, and the results show that the structure maintains RF-transparency.
A Phase I Clinical and Pharmacokinetic Study of Oral CI-1033 in Combination with Docetaxel in Patients with Advanced Solid Tumors (2006)

It is feasible to combine the irreversible pan-erbB tyrosine kinase inhibitor CI-1033 with docetaxel on an intermittent dosing schedule in advanced cancer patients. We established the maximum tolerated dose and recommended phase II dose for the combination. Further investigation of this combination should include a rigorous analysis of the effect of docetaxel on CI-1033 pharmacokinetics.
Dual‐energy CT quantitative imaging: a comparison study between twin‐beam and dual‐source CT scanners (2017)

Spatial resolution is similar for the three scanners. The twin-beam is able to derive ρ<sub>e</sub> and Z<sub>eff</sub> , but with inferior accuracy compared to both dual-source scanners.
Performance assessment of total RNA sequencing of human biofluids and extracellular vesicles (2019)

Abstract RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, RNA sequencing methods to quantify the total RNA content outside cells are rare. Here, we evaluate the performance of the SMARTer Stranded Total RNA-Seq method in human platelet-rich plasma, platelet-free plasma, urine, conditioned medium, and extracellular vesicles (EVs) from these biofluids. We found the method to be accurate, precise, compatible with low-input volumes and able to quantify a few thousand genes. We picked up distinct classes of RNA molecules, including mRNA, lncRNA, circRNA, miscRNA and pseudogenes. Notably, the read distribution and gene content drastically differ among biofluids. In conclusion, we are the first to show that the SMARTer method can be used for unbiased unraveling of the complete transcriptome of a wide range of biofluids and their extracellular vesicles.
Quest for the high-field phase of CdCr2O4 using an X-ray diffraction technique (2006)

Single-crystal x-ray diffraction experiments on the geometrically frustrated magnet CdCr2O4 have been carried out under pulsed magnetic fields up to 31 T. By applying magnetic fields, CdCr2O4 exhibits a magnetization plateau which is one-half of the saturated magnetization above 28 T, and it is expected that a structural transition from a tetragonal to a rhombohedral or cubic structure occurs simultaneously through a so-called "spin Jahn-Teller" mechanism. Our diffraction experiments confirmed that a sharp structural transition occurs at 28 T and that the 440 reflection is a single peak, suggesting that the crystal structure of the high-field plateau phase is cubic.
Digital Assistant Power Integrated Technologies for PMU in Scaling CMOS Process (2013)

Several novel digital assistant power integrated technologies (DAPITs) for power management unit (PMU) to reduce the designing complexity, as well as to keep good performance in scaling CMOS process, are proposed in this paper. The DAPITs include segment power driving with pulse skip modulation/pulse width modulation and loop regulating with digital regulation circuit for dc-dcs in PMU, calibrating digital-to-analog converter (DAC) with resistance compensation network for dynamic voltage scaling (DVS), and separating phase clock for multi-dc-dcs in PMU. With these DAPITs, the efficiencies and output accuracies of dc-dcs are increased, the DVS signal sent by DAC is linear, and current ripples in PMU are reduced. In this paper, a PMU embedded into system-on-a-chip (SoC) is designed based on a 0.13-μm CMOS process. The designed PMU includes four dc-dc buck converters and two LDOs. This paper introduces the top structure of the PMU and the main proposed DAPITs. Simulation and test results show that the output voltages of the dc-dc converters in the PMU can be changed by external resistors and regulated from 0.7 to 1.8 V stepped with 25 mV by SoC load through interface. And the maximum efficiency of the dc-dcs with DVS in the PMU is more than 90%, while, with an external resistor, it can reach to 95%.
Novel Mouse Model of a Human Mutation in NKCC1 Confirms its Mistargeting in Epithelia (2018)

A 15‐year old patient with complete gastrointestinal and bladder failure, endocrine insufficiencies, and orthostatic intolerance carries a de novo mutation in SLC12A2, the gene encoding the Na‐K‐2Cl cotransporter‐1 or NKCC1. The 11 bp deletion leads to truncation of the cytosolic COOH‐terminal tail of the cotransporter making it non‐functional . Expression of the mutant transporter increases the amount of dimerization, suggesting the possibility that the mutant transporter might exert dominant‐negative effects on the wild‐type transporter. In polarized MDCK cells grown on permeable filters or in 3‐D culture cysts, most of the mutant transporter signal is observed on the apical membrane, in contrast to the basolateral expression of wild‐type cotransporter. The mutant transporter affects the formation of MDCK cysts, as we observed the presence of multiple lumens, as opposed to single lumen formation with wild‐type cells. The mutant transporter also causes trafficking of the wild‐type cotransporter to the apical membrane. To assess the relevance of this observation in vivo , we created a mouse model that recapitulates the human mutation. Using CRISPR/cas9, a guide RNA that targets cas9 to exon 22 of the Slc12a2 gene, and a repair oligonucleotide that deletes 11 bp from the exon, we successfully generated a female founder animal that carried the designed mutant allele in addition to a non‐homologous end joining mutation in the second allele. After one backcross that segregated the two alleles, the line was further established by backcrossing for 2 additional generations in C57BL/6J. This mouse now carries the 11 bp deletion mutant allele and a wild‐type allele. We examined the expression of NKCC1 in the salivary gland and the intestine, two Cl − secreting epithelia where the wild‐type cotransporter is typically expressed on the basolateral membrane. In both tissues, intense apical staining was observed, confirming the data obtained with MDCK cells. Preliminary studies indicate that the mouse has no overt adverse phenotype, although the mouse lives in a pathogen‐free barrier facility and has not been challenged. In conclusion, our data demonstrate that expression of a truncated Na‐K‐2Cl cotransporter causes mistargeting of the wild‐type cotransporter to the apical membrane of Cl − secreting epithelia and affects proper lumen formation in MDCK cysts. These observations may explain the multisystem dysfunction that is observed in the patient. Support or Funding Information Supported by NIH grants R21GM118944 and 1RO1DK093501. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
Tunable acoustic absorbing system using a deep hole array (2002)

We present a tunable acoustic absorbing system. Most of the conventional acoustic absorbers can absorb an only sound of a fixed frequency. We propose an acoustic absorbing system with a tunable compliance in order to absorb variable sounds. The developed system forms an array of the Helmholtz resonator with a deep hole array by a X-ray lithography. Micro deep holes are effective at a low frequency. The resonator has an air cavity whose volume is controllable so as to tune its resonant frequency to a desired frequency. This paper reports characteristics of our developed acoustic absorbing system and our project status.
Structural basis of the affinity for oligomannosides and analogs displayed by BC2L-A, a Burkholderia cenocepacia soluble lectin (2009)

The opportunistic pathogen Burkholderia cenocepacia contains three soluble carbohydrate-binding proteins, related to the fucose-binding lectin PA-IIL from Pseudomonas aeruginosa. All contain a PA-IIL-like domain and two of them have an additional N-terminal domain that displays no sequence similarities with known proteins. Printed arrays screening performed on the shortest one, B. cenocepacia lectin A (BC2L-A), demonstrated the strict specificity for oligomannose-type N-glycan structures (Lameignere E, Malinovská L, Sláviková M, Duchaud E, Mitchell EP, Varrot A, Sedo O, Imberty A, Wimmerová M. 2008. Structural basis for mannose recognition by a lectin from opportunistic bacteria Burkholderia cenocepacia. Biochem J. 411:307-318.). The disaccharides alphaMan1-2Man, alphaMan1-3Man, and alphaMan1-6Man and the trisaccharide alphaMan1-3(alphaMan1-6)Man were tested by titration microcalorimetry in order to evaluate their affinity for BC2L-A in solution and to characterize the thermodynamics of the binding. Oligomannose analogs presenting two mannoside residues separated by either flexible or rigid spacer were also tested. Only the rigid one yields to high affinity binding with a fast kinetics of clustering, while the flexible analog and the trimannoside display moderate affinities and no clustering effect on short time scale. The crystal structures of BC2L-A have been obtained in complex with alphaMan1-3Man disaccharide and alphaMan1(alphaMan1-6)-3Man trisaccharide. The lengthy time required for the co-crystallization with the trisaccharide allowed for the formation of cluster since in the BC2L-A-trimannose complex solved at 1.1 A resolution, the sugar creates a bridge between two adjacent dimers, yielding to molecular strings. AFM experiments were performed in order to visualize the filaments formed in solution by this type of interaction.
Improved, Selective, Human Intestinal Carboxylesterase Inhibitors Designed to Modulate 7-Ethyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxycamptothecin (Irinotecan; CPT-11) Toxicity (2009)

CPT-11 is an antitumor prodrug that is hydrolyzed by carboxylesterases (CE) to yield SN-38, a potent topoisomerase I poison. However, the dose limiting toxicity delays diarrhea that is thought to arise, in part, from activation of the prodrug by a human intestinal CE (hiCE). Therefore, we have sought to identify selective inhibitors of hiCE that may have utility in modulating drug toxicity. We have evaluated one such class of molecules (benzene sulfonamides) and developed QSAR models for inhibition of this protein. Using these predictive models, we have synthesized a panel of fluorene analogues that are selective for hiCE, demonstrating no cross reactivity to the human liver CE, hCE1, or toward human cholinesterases, and have K(i) values as low as 14 nM. These compounds prevented hiCE-mediated hydrolysis of the drug and the potency of enzyme inhibition correlated with the clogP of the molecules. These studies will allow the development and application of hiCE-specific inhibitors designed to selectively modulate drug hydrolysis in vivo.
The Effect of Surfactant HLB on the Self-Emulsifying Efficiency of Non-Ionic Surfactant-Vegetable Oil Mixtures (1986)

Journal Article The Effect of Surfactant HLB on the Self-Emulsifying Efficiency of Non-Ionic Surfactant-Vegetable Oil Mixtures Get access M G Wakerly, M G Wakerly School of Pharmacy and Pharmacology, University of Bath, Avon Search for other works by this author on: Oxford Academic Google Scholar C W Pouton, C W Pouton School of Pharmacy and Pharmacology, University of Bath, Avon Search for other works by this author on: Oxford Academic Google Scholar B J Meakin, B J Meakin School of Pharmacy and Pharmacology, University of Bath, Avon Search for other works by this author on: Oxford Academic Google Scholar F S Morton F S Morton R. P. Scherer Ltd, Swindon, Wilts Search for other works by this author on: Oxford Academic Google Scholar Journal of Pharmacy and Pharmacology, Volume 38, Issue Supplement_12, December 1986, Page 2P, https://doi.org/10.1111/j.2042-7158.1986.tb14231.x Published: 12 April 2011
Managing liver transplantation during the COVID-19 pandemic: A survey among transplant centers in the Southeast United States (2021)

The COVID-19 pandemic greatly affected liver transplantation in the southeastern United States. It was evident that a concerted effort was made by LT centers to protect their patients and employees from COVID-19 but also to continue the life-saving procedure of LT in this sick patient population. Further studies are needed to assess how LT centers around the world managed the pandemic in order to learn strategies to continue life-saving procedures in this patient population.
Sugar analog synthesis by in vitro biocatalytic cascade: A comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis (2017)

Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formation with the generation of two new stereo-centres, with access to all four possible stereoisomers of a compound. Dihydroxyacetone phosphate (DHAP) is unstable so the provision of DHAP for DHAP-dependent aldolases in biocatalytic processes remains complicated. Our research has investigated the efficiency of several different enzymatic cascades for the conversion of glycerol to DHAP, including characterising new candidate enzymes for some of the reaction steps. The most efficient cascade for DHAP production, comprising a one-pot four-enzyme reaction with glycerol kinase, acetate kinase, glycerophosphate oxidase and catalase, was coupled with a DHAP-dependent fructose-1,6-biphosphate aldolase enzyme to demonstrate the production of several rare chiral sugars. The limitation of batch biocatalysis for these reactions and the potential for improvement using kinetic modelling and flow biocatalysis systems is discussed.
Molecular analysis of the apoptotic effects of BPA in acute myeloid leukemia cells (2009)

BPA is able to induce apoptosis in leukemia cells via caspase activation and involvement of both intrinsic and extrinsic pathways of apoptosis.
Prediction of neural excitation during magnetic stimulation using passive cable models (2000)

A method for predicting neural excitation during magnetic stimulation using passive cable models has been developed. This method uses the information of the threshold capacitor voltage for magnetic stimulation coils to determine the equivalent excitation thresholds for the passive transient (PT) and passive steady-state (PSS) cable models as well as for the activating function. The threshold values for the PT, PSS models, and the activating function vary only with the pulsewidth of the stimulus for a variety of coils at different locations and orientations. Furthermore, the excitation threshold for the PSS model is also independent of axon diameter and best fitted to a simple mathematical function. By comparing the transmembrane potential of the PSS model with the corresponding threshold, the prediction of excitation during magnetic stimulation can be made. Similarly, it is also possible to predict excitation using the PT model and the activating function with the corresponding thresholds provided. By taking advantage of the weighted pulsewidth, this method can even predict the excitation for stimuli with various waveforms, greatly simplifying the determination of neural excitation for magnetic stimulation.
Tanks 18 and 19-F equipment grout fill material evaluation and recommendations (2011)

The United States Department of Energy (US DOE) intends to remove Tanks 18-F and 19-F at the Savannah River Site (SRS) from service. The high-level waste (HLW) tanks have been isolated from the F-area Tank Farm (FTF) facilities and will be filled with cementitious grout for the purpose of: (1) physically stabilizing the empty volumes in the tanks, (2) limiting/eliminating vertical pathways from the surface to residual waste on the bottom of the tanks, (3) providing an intruder barrier, and (4) providing an alkaline, chemical reducing environment within the closure boundary to limit solubility of residual radionuclides. Bulk waste and heel waste removal equipment will remain in Tanks 18-F and 19-F when the tanks are closed. This equipment includes: mixer pumps, transfer pumps, transfer jets, equipment support masts, sampling masts and dip tube assemblies. The current Tank 18-F and 19-F closure strategy is to grout the internal void spaces in this equipment to eliminate fast vertical pathways and slow water infiltration to the residual material on the tank floor. This report documents the results of laboratory testing performed to identify a grout formulation for filling the abandoned equipment in Tanks 18-F and 19-F. The objective of this work was to formulate a flowable grout for filling internal voids of equipment that will remain in Tanks 18-F and 19-F during the final closures. This work was requested by V. A. Chander, Tank Farm Closure Engineering, in HLW-TTR-2011-008. The scope for this task is provided in the Task Technical and Quality Assurance Plan (TTQAP), SRNL-RP-2011-00587. The specific objectives of this task were to: (1) Prepare and evaluate the SRR cooling coil grout identified in WSRC-STI-2008-00298 per the TTR for this work. The cooling coil grout is a mixture of BASF MasterFlow{reg_sign} 816 cable grout (67.67 wt. %), Grade 100 ground granulated blast furnace slag (7.52 wt. %) and water (24.81 wt. %); (2) Identify equipment grout placement and performance properties; (3) Design up to 2 additional grout systems for filling the Tank 18-F and Tank 19-F equipment; (4) Prepare samples of candidate grouts and measure fresh properties, thermal properties and cured properties; (5) Recommend a grout for the Tier 1A equipment fill mock up - ADMP 4 foot high mock up, 1 inch and 2 inch pipes; (6) Support procurement of materials for the Tier 1A equipment fill mock up test; (7) Prepare samples of the recommended grout for hydraulic property measurements which can be used for comparison to values used in the F- Tank Farm Performance Assessment (PA); and (8) Document equipment fill grout data and recommendations in a report.
Measurement of Aldosterone in Human Plasma by Semiautomated HPLC–Tandem Mass Spectrometry (2009)

This first reported aldosterone method using online HPLC-MS/MS is precise across the clinically relevant range, not influenced by collection tube type, and offers semiautomated sample preparation and high throughput.
[Effect of dehydration on lipid metabolism]. (1978)

Control of 25 sportsmen-wrestlers determined that accelerated loss in weight leads to a significant increase of ketone bodies evacuation with urine. The loss in weight for a 10-11 days period before a competition results in a less pronounced hyperketonuria. In experiment on rats it is shown that 48 and 98 hour hyperthermia and dehydration lead to the inhibition of the glucose-6-phosphate dehydrogenase activity in the liver. The mentioned permits supposing that under indicated conditions the inhibition of fat acid synthesis caused by NADPH2 and an increase of ketone bodies and cholesterol synthesis are observed.
OnpA, an Unusual Flavin-Dependent Monooxygenase Containing a Cytochrome b 5 Domain (2012)

ortho-Nitrophenol 2-monooxygenase (EC 1.14.13.31) from Alcaligenes sp. strain NyZ215 catalyzes monooxygenation of ortho-nitrophenol to form catechol via ortho-benzoquinone. Sequence analysis of this onpA-encoded enzyme revealed that it contained a flavin-binding monooxygenase domain and a heme-binding cytochrome b(5) domain. OnpA was purified to homogeneity as a His-tagged protein and was considered a monomer, as determined by gel filtration. FAD and heme were identified by high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry (HPLC-MS) as cofactors in this enzyme, and quantitative analysis indicated that 1 mol of the purified recombinant OnpA contained 0.66 mol of FAD and 0.20 mol of heme. However, the enzyme activity of OnpA was increased by 60% and 450% after addition of FAD and hemin, respectively, suggesting that the optimal stoichiometry was 1:1:1. In addition, site-directed mutagenesis experiments confirmed that two highly conserved histidines located in the cytochrome b(5) domain were associated with binding of the heme, and the cytochrome b(5) domain was involved in the OnpA activity. These results indicate that OnpA is an unusual FAD-dependent monooxygenase containing a fused cytochrome b(5) domain that is essential for its activity. Therefore, we here demonstrate a link between cytochrome b(5) and flavin-dependent monooxygenases.