gebhart/patrepeval huggingface dataset:
Morphological and Physiological Characterization of Layer VI Corticofugal Neurons of Mouse Primary Visual Cortex (2003)

Layer VI is the origin of the massive feedback connection from the cortex to the thalamus, yet its complement of cell types and their connections is poorly understood. The physiological and morphological properties of corticofugal neurons of layer VI of mouse primary visual cortex were investigated in slices loaded with the Ca(2+) indicator fura-2AM. To identify corticofugal neurons, electrical stimulation of the white matter (WM) was done in conjunction with calcium imaging to detect neurons that responded with changes in intracellular Ca(2+) concentrations in response to the stimulation. Subsequent whole cell recordings confirmed that they discharged antidromic action potentials after WM stimulation. Antidromically activated neurons were more excitable and had different spiking properties than neighboring nonantidromic neurons, although both groups had similar input resistances. Furthermore, antidromic neurons possessed narrower action potentials and smaller afterhyperpolarizations. Additionally, three-dimensional reconstructions indicated that antidromically activated neurons had a distinct morphology with longer apical dendrites and fewer nonprimary dendrites than nonantidromic cells. To identify the antidromic neurons, rhodamine microspheres were injected into the dorsal lateral geniculate nucleus of the thalamus and allowed to retrogradely transport back to the somata of the layer VI cortico-geniculate neurons. Physiological and anatomical analysis indicated that most antidromic neurons were likely to be cortico-geniculate neurons. Our results show that cortico-thalamic neurons represent a specific functional and morphological class of layer VI neurons.
Radially polarized nanosecond Yb-doped fiber MOPA system incorporating temporal shaping (2016)

580μJ nanosecond pulses at a repetition rate of 25kHz with user-defined temporal pulse shapes and a radially polarized doughnut-shaped spatial mode have been demonstrated in an Yb-doped fiber MOPA system seeded by a super-luminescent diode.
General transposed series-parallel switched capacitor converter topologies (2017)

This paper considers a unique type of multitude DC/DC conversion ratios switched capacitor converter. The multitude conversion ratios enable the converter to be able to be almost continuous and competitive to inductive based converters. The paper summarizes the basic rules of the topology as well as presenting two possible ways of implementing the topology. The paper also discusses the difference between the series type topology and the parallel one as well as the reasons to implement the latter type of converter. The topologies are presented theoretically as well as the actual prototype characteristics and experimental results.
Isoelectric Focusing Under Denaturing Conditions (1996)

The introduction of methods for the electrophoretic analysis of proteins under denaturing conditions (,) is something of a landmark in the development of methodologies for the analysis of proteins. Not only was analysis giving good correlation to molecular weight possible with small samples of complex mixtures of proteins, but in addition, analysis of normally insoluble proteins became possible in a relatively simple manner. These methods were based on solubilizing protein in sodium dodecyl sulfate (SDS) such that the intrinsic charge differences between proteins were overwhelmed by the relatively strong charge of the dodecyl sulfate ion, which resulted in very good correlation (for most, but not all, proteins) between electrophoretic mobility and molecular weight (see Chapter 11).
Multi-view clustering via canonical correlation analysis (2009)

Clustering data in high dimensions is believed to be a hard problem in general. A number of efficient clustering algorithms developed in recent years address this problem by projecting the data into a lower-dimensional subspace, e.g. via Principal Components Analysis (PCA) or random projections, before clustering. Here, we consider constructing such projections using multiple views of the data, via Canonical Correlation Analysis (CCA).Under the assumption that the views are un-correlated given the cluster label, we show that the separation conditions required for the algorithm to be successful are significantly weaker than prior results in the literature. We provide results for mixtures of Gaussians and mixtures of log concave distributions. We also provide empirical support from audio-visual speaker clustering (where we desire the clusters to correspond to speaker ID) and from hierarchical Wikipedia document clustering (where one view is the words in the document and the other is the link structure).
Fast approximate i-vector estimation using PCA (2015)

The i-vector representation has become increasingly popular in speaker and language recognition systems. The estimation of the projection matrix of the i-vector model is usually performed using the iterative expectation maximization (EM) algorithm. This work presents a novel approach to estimate the projection matrix of the i-vector representation and to estimate the i-vector representation for each utterance. In this approach, we formulate the estimation of the projection matrix as a principal component analysis (PCA) problem. Using the relation between PCA and a linear Gaussian model trained using the EM algorithm, we show that an approximate solution of the i-vector estimation can be obtained as the solution of a PCA problem. We evaluate the performance of our approximate i-vector estimation on the language recognition task of the robust automatic transcription of speech (RATS) project. The proposed approach reduces by 50% relative the computational time required to estimate the i-vector projection matrix and by 42% relative the computational time to estimate the i-vector representation compared to the standard EM-based approach to i-vector estimation. In addition, our experiments show improvements up to 29% relative in language recognition performance in terms of equal error rate compared to the standard EM-based i-vector estimation.
Roles of ERK and cPLA2 in the Angiotensin II-Mediated Biphasic Regulation of Na+-HCO3 − Transport (2007)

Regulation of renal proximal transport by angiotensin II (Ang II) is biphasic: low concentrations (picomolar to nanomolar) stimulate reabsorption, but higher concentrations (nanomolar to micromolar) inhibit reabsorption. Traditionally, the stimulatory effect has been attributed to activation of protein kinase C and/or a decrease in intracellular cAMP, whereas the inhibitory action has been attributed to the activation of phospholipase A2 (PLA2) and the subsequent release of arachidonic acid. The Ang II receptor subtype responsible for these effects and the intracellular signaling pathways involved are not completely understood. We isolated proximal tubules from wild-type, Ang II type 1A receptor (AT1A)-deficient, and group IVA cytosolic phospholipase A2 (cPLA2alpha)-deficient mice, and compared their responses to Ang II. In wild-type mice, we found that the stimulatory and inhibitory effects of Ang II on Na+-HCO3(-) cotransporter activity are both AT1-mediated but that ERK activation only plays a role in the former. The stimulatory effect of Ang II was also observed in AT1A-deficient mice, suggesting that this occurs through AT1B. In contrast, the inhibitory effects of Ang II appeared to be mediated by cPLA2alpha activation because high-concentration Ang II stimulated Na+-HCO3(-) cotransporter activity when cPLA2alpha activity was abrogated by pharmacological means or genetic knockout. Consistent with this observation, we found that activation of the cPLA2alpha/P450 pathway suppressed ERK activation. We conclude that Ang II activates ERK and cPLA2alpha in a concentration-dependent manner via AT1, and that the balance between ERK and cPLA2alpha activities determines the ultimate response to Ang II in intact proximal tubules.
Expression of Cytokines in Bacterial and Viral Infections and Their Biochemical Aspects (2000)

Cytokines are very important in the host defense system, and play a critical role in protection against bacterial and viral infections. Cytokines are also involved in the pathogenesis and development of symptoms in infections. In this article, Helicobacter pylori (H. pylori) infection as bacterial infection, and influenza virus infection, encephalomyocarditis virus (EMCV) infection, and herpes simplex virus (HSV) infection as viral infection are mentioned. In H. pylori infection, various chemokines, especially interleukin (IL)-8, induce inflammatory responses in the gastroduodenal mucosa. Furthermore, IL-6, IL-7, tumor necrosis factor (TNF)-alpha, and interferon (IFN)-gamma are involved in both protection and pathogenesis. In influenza virus infection, IFN-alpha/beta, IFN-gamma, and IL-6 play protective roles. In EMCV infection, IL-6 and TNF-alpha play important roles as a protective and exacerbative factor in acute myocarditis, respectively. Furthermore, in HSV infection, the production of inflammatory cytokines is closely correlated with the pathogenesis of herpetic keratitis, and IFN-gamma plays an important role in enhancing viral clearance from the cornea and trigeminal ganglions.
Effect ofN-linked glycosylation on the activity and other properties of recombinant endoglucanase IIa (Cel5A) fromPenicillium verruculosum (2016)

Endoglucanase IIa from Penicillium verruculosum (PvCel5A) has three potential N-glycosylation sites: Asn19, Asn42 and Asn194. In order to study the role of N-glycosylation, the wild type (wt) PvCel5A and its mutant forms, carrying Asn to Ala substitutions, were cloned into Penicillium canescens. All forms of the rPvCel5A were successfully expressed and purified for characterization. The MALDI-TOF mass spectrometry peptide fingerprinting showed that N-glycans linked to Asn42 and Asn194 represent variable oligosaccharides, according to the formula (Man)1-9(GlcNAc)2. No evidence for Asn19 glycosylation was found. Mutations had no notable effect on the enzyme thermostability; however, the N-linked glycans stabilized the enzyme against proteolytic attack. For N42A and N194A mutants, a slight shift of pH-optimum to pH 5.0 was observed (from pH-optimum of 4.5 for the native enzyme, rPvCel5A-wt and N19A mutant). The N19A mutation led to a notable decrease in the specific activity against carboxymethylcellulose and barley β-glucan (by 26% and 12% relative to the rPvCel5A-wt), while the N42A and N194A mutants displayed 12-13% and 32-35% increase in the activities. Similar effects of the mutations were observed in prolonged hydrolysis of β-glucan and milled aspen wood by rPvCel5A forms in the presence of purified β-glucosidase.
Real-Time Optical Associative Retrieval Technique (1986)

A real-time optical associative retrieval technique is presented. The associative retrieval model enables a large amount of data to be stored and recalled by partial information optically in real time. The real-time capability is achieved by using an electronically addressed spatial light modulator based on the pocket-size liquid crystal display television. The potential application of the technique to the perceptive vision requirements in telerobotics for achieving NASA's goals of automation in space is described.
The roles of specific xanthophylls in photoprotection (1997)

Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the green alga Chlamydomonas reinhardtii revealed functions for specific xanthophylls in the nonradiative dissipation of excess absorbed light energy, measured as nonphotochemical quenching of chlorophyll fluorescence. Mutants with a defect in either the alpha- or beta-branch of carotenoid biosynthesis exhibited less nonphotochemical quenching but were still able to tolerate high light. In contrast, a double mutant that was defective in the synthesis of lutein, loroxanthin (alpha-carotene branch), zeaxanthin, and antheraxanthin (beta-carotene branch) had almost no nonphotochemical quenching and was extremely sensitive to high light. These results strongly suggest that in addition to the xanthophyll cycle pigments (zeaxanthin and antheraxanthin), alpha-carotene-derived xanthophylls such as lutein, which are structural components of the subunits of the light-harvesting complexes, contribute to the dissipation of excess absorbed light energy and the protection of plants from photo-oxidative damage.
Radioreceptor Assay of Insulin: Comparison of Plasma and Pancreatic Insulins and Proinsulins (1975)

Porcine proinsulin, related intermediates and plasma immunoreactive insulin components have been studied by radioreceptor assay. Using the purified rat liver membrane or cultured human lymphocyte radioreceptor assay, porcine proinsulin is %5, split proinsulin 6% (54-55 split in connecting peptide) desdipeptide proinsulin 20% (deletion of amino acids 62 and 63 of connecting peptide) and desnonapeptide proinsulin 27% (deletion of amino acids 55-63 of connecting peptide) as active as porcine insulin in both assay systems; these values closely parallel the in vitro bioactivity of these preparations. In the lymphocyte radioreceptor assay the human plasma immunoreactive insulin-like component has the same potency as porcine insulin per immunoreactive unit, whereas the plasma immunoreactive proinsulin-like component is only 15% as active. Since both plasma immunoreactive components are somewhat less reactive than would be expected from puriified human insulin and proinsulin, the data suggest that both plasma components contain immunoreactive molecules that do not react in the radioreceptor assay.
Computational lithography and its role in nanoimprint lithography for semiconductor device manufacturing (2020)

For nanoimprint lithography, computational technologies are still being developed. In this paper, we introduce a new NIL process simulator which simulates the whole imprinting process, and evaluates the quality of the resulting resist film. To overcome the scale difference of each component of the system, which makes it difficult to calculate the process with conventional fluid structure interaction simulators, our simulator utilizes analytically integrated expressions which reduce the dimensions of the calculation region. Additionally, we report on the critical dimension uniformity of sub-20nm contact holes as a demonstration of pattern robustness and discuss advancements made in defectivity, throughput and overlay.
Development of Abutment Design Standards for Local Bridge Designs. Volume 2 of 3: Design Manual (2004)

Several superstructure design methodologies have been developed for low volume road bridges by the Iowa State University Bridge Engineering Center. However, to date no standard abutment designs have been developed. Thus, there was a need to establish an easy to use design methodology in addition to generating generic abutment standards and other design aids for the more common substructure systems used in Iowa. The final report for this project consists of three volumes. The second volume (this volume) introduces and outlines the use of the various design aids developed for this project. Charts for determining dead and live gravity loads based on the roadway width, span length, and superstructure type are provided. A foundation design template was developed in which the engineer can check a substructure design by inputting basic bridge site information. Tables published by the Iowa Department of Transportation that provide values for estimating pile friction and end bearing for different combinations of soils and pile types are also included. Generic standard abutment plans were developed for which the engineer can provide necessary bridge site information in the spaces provided. These tools enable engineers to design and detail county bridge substructures more efficiently.
Demonstrating the Resilience of Geographical Routing to Localization Errors (2007)

This demonstration concerns geographic forwarding (GF) as an effective solution for data dissemination (from sensors to a sink) in wireless sensor networks (WSNs). In particular, we focus on demonstrating the different degrees of resilience of a recent solution, ALBA-R, to localization errors, which are highly likely to occur in WSNs. GF routing protocols are based on the nodes knowing their own location information as well as that of the sink, which is the intended destination of a packet.
Effect of Polyunsaturated Fatty Acids on Fetal Mouse Brain Cells in Culture in a Chemically Defined Medium (1983)

The biochemical and morphological effects of polyunsaturated fatty acids on fetal brain cells grown in a chemically defined medium were studied. Fetal brain cells were dissociated from mouse cerebral hemispheres taken on the 16th day of gestation. After cells had grown in chemically defined medium for 8 days, the proportion of polyunsaturated fatty acids of cultured cells was only one-half of that observed at day 0 and about 1.5 times less than that of cells grown in serum-supplemented medium. Fatty acid 20:3(n-9) was present in cultured cells grown in either chemically defined or serum-supplemented medium, demonstrating the deficiency of essential fatty acids. The reduced amount of polyunsaturated fatty acids in cells grown in the chemically defined medium was balanced by an increase in monounsaturated fatty acids. The saturated fatty acids were not affected. When added at the seeding time, linoleic, linolenic, arachidonic, or docosahexaenoic acid stimulated the proliferation of small dense cells. Besides, we demonstrate that each of the four fatty acids studied was incorporated into phospholipids. Adding fatty acids of the n-6 series increased the content of n-6 fatty acids in the cells, but also provoked an increase in the n-3 fatty acids.(ABSTRACT TRUNCATED AT 250 WORDS)
Design Patterns for Reconfigurable Computing (2004)

It is valuable to identify and catalog design patterns for reconfigurable computing. These design patterns are canonical solutions to common and recurring design challenges which arise in reconfigurable systems and applications. The catalog can form the basis for creating designs, for educating new designers, for understanding the needs of tools and languages, and for discussing reconfigurable design. Tying application and implementation lessons to the expansion and refinement of this catalog make those lessons more relevant to the design community. In this paper, we articulate this role for design patterns in reconfigurable computing, provide a few example patterns, offer a starting point for the contents of the catalog, and discuss the potential benefits of this effort.
ApoA-1 in Diabetes: Damaged Goods (2010)

Diabetes is a major risk factor for the development of atherosclerosis. In addition to increased risk of stroke, myocardial infarction, and peripheral vascular disease, diabetics suffer from a particularly aggressive form of atherosclerosis with greater in-hospital mortality following myocardial infarction and a higher incidence of heart failure, if they survive (1–3). While diabetics often have other accompanying risk factors for atherosclerosis (e.g., hypertension hypercholesterolemia, obesity), the additional risk conferred by diabetes and the particularly aggressive vascular and myocardial disease that affects diabetics suggest that diabetes-associated atherosclerosis involves unique pathogenic mechanisms.

The systemic metabolic disturbances of diabetes, including hyperglycemia and hyperlipidemia, likely play a central role in the pathogenesis of diabetes-associated atherosclerosis through the generation of oxidative stress. Hyperglycemia causes increased flux through the polyol pathway, formation of advanced glycation end products, activation of protein kinase C isoforms, and increased hexosamine pathway flux, all of which may contribute to increased oxidative stress (4–6). Excessive free fatty acids delivered to nonadipose tissues can lead to reactive oxygen species (ROS) formation through cycles of oxidative phosphorylation, activation of NADPH oxidase, and alterations in mitochondrial structure that precipitate ROS production (7–9). In addition to evidence for activation of these pathways in cultured endothelial cells, human studies support the notion of increased systemic oxidative stress in diabetic subjects in …
Recent Developments in SOBEK Passive Sonar Technology (2012)

Surveillance of waterside locations for protection against threats from small fast surface vessels and underwater intruders is a very relevant but challenging problem. For this reason, the Netherlands Organisation for Applied Scientific Research (TNO) is developing SOBEK â a family of passive sonar technologies for waterside surveillance. The advantages of passive sonar are several-fold: it is low-cost, it allows covert operation, it is not harmful to underwater life, and the reverberation, which can pose significant challenges for active sonar in confined water, is absent. Furthermore, passive acoustic signatures provide a wealth of information for target classification. This paper presents recent developments in our SOBEK passive sonar research, including new results from a 2011 sea trial in the Dutch Navy harbour of Den Helder, and an overview of future system concepts.
Velocity measurement by means of mechanical movement of a detector (1995)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation A. V. Feschenko, P. N. Ostroumov; Velocity measurement by means of mechanical movement of a detector. AIP Conf. Proc. 5 May 1995; 333 (1): 478–484. https://doi.org/10.1063/1.48027 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Growth‐promoting activity of tissue inhibitor of metalloproteinases‐1 (TIMP‐1) for a wide range of cells A possible new growth factor in serum (1992)

Human tissue inhibitor of metalloproteinases-1 (TIMP-1), but not TIMP-2, has potent growth-promoting activity for a wide range of human and bovine cells. TIMP-1 seems to be a new cell-growth factor in serum and to stimulate the cells independently of its inhibitory activity.
Chlorosulfonic Acid Catalyzed One‐Pot Synthesis of Coumarin. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.