gebhart/patrepeval huggingface dataset:
Effects of Carboxyl-terminal Truncations on the Activity and Solubility of Human Monoamine Oxidase B (2001)

Monoamine oxidase is an outer mitochondrial membrane protein that catalyzes the deamination of a number of neurotransmitters and dietary amines. To determine the roles of the carboxyl-terminal amino acids on the activity and solubility of human monoamine oxidase (MAO B), 10 sequential mutants were made with stop codons at amino acid positions 511, 504, 498, 492, 486, 481, 476, 467, 417, and 397, respectively. All truncated mutants were expressed in Sf21 insect cells using baculovirus, and the enzyme kinetic parameters were determined. Truncations at amino acid positions 511, 504, and 498 slightly decreased MAO B catalytic activity and had no significant changes on deprenyl inhibition. Further deletions up to amino acid 417 decreased the specific activity 10--100-fold without significant changes of the K(m) for phenylethylamine or dopamine or the IC(50) for deprenyl and clorgyline. The truncation mutant C397, which lacks covalently attached FAD, was inactive. Progressive carboxyl-terminal truncations up to position 481 were correlated with increased solubility of MAO B mutants. 47% of the activity of the truncated C481 was found in the 105,000 x g supernatant in the absence of detergent. However, further truncated mutants, C476, C467, and C417, remained associated with the membrane fraction. In contrast to crude homogenate, the water-soluble C481 mutant was rapidly inactivated at 4 degrees C and 37 degrees C, which indicates that the membrane environment is required for the stability of MAO B. Expression of the green fluorescent protein-MAO B C481 fusion protein revealed that this mutant was located in the cytoplasm, whereas its counterpart in MAO A, truncated mutant C490, was located on the mitochondria. These results suggest that the carboxyl-terminal amino acid residues 417--520 of MAO B are not directly involved in the active site but are required for maintaining the appropriate conformation and interaction with the outer mitochondrial membrane. The different solubilities of the various carboxyl-terminal truncation mutants indicate that the interaction of MAO B with mitochondrial membrane is not simply anchoring through the carboxyl-terminal hydrophobic tail. Further, our results suggest that the carboxyl-terminal of MAO A and B plays different roles in mitochondrial attachment.
Impact of neuron network on the generation of chaotic attractors (2022)

In this paper, new models of chaotic attractors are presented. In doing so, an old chaotic attractor based on the mathematical model of a Variable Structure Model of Neurons (VSMN) is developed. It is then generated by neuron with oscillators. As a result, new styles of new chaotic attractors are found. Also, a detailed bifurcation analysis of those new chaotic systems with theory and simulations is discussed. Finally, the numerical examples will illustrate each model with its simulation using Python. We found out that the new systems are chaotic and could be used for different applications like image encryption. Bifurcation, Chaotic attractor, neuron, oscillators, Variable Structure Model of Neurons.
Disparity based compression technique for focused plenoptic images (2014)

In this paper we present a novel method for compression of focused plenoptic (light field) images. Plenoptic image is an array of lenslet images where the consecutive images act as stereo images. Adjacent lenslet images have overlapping regions leading to higher levels of redundancy than observed in a normal image. We use this overlap to appropriately select a set of reference images from which the remaining lenslet images can be approximately reconstructed. These reference images can be compressed using jpeg. The residue of the reconstructed image from the original image is compressed using either DWT-SPIHT or DCT based compression. Using the proposed method we are able to compress the image at high PSNR at a low bits per pixel (bpp).
Supplementary Table S1 from A Critical Role for Fas-Mediated Off-Target Tumor Killing in T-cell Immunotherapy (2023)

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Four-Dimensional Flow Magnetic Resonance Imaging Evaluation of Post–Ross David Procedure With Valve Repair (2023)

Valve-sparing aortic root replacements have acceptable reintervention rates in patients with failed pulmonary autografts after a Ross procedure. In our 50-year-old patient with post-Ross valve-sparing aortic root replacement, we report preoperative and postoperative 4-dimensional flow magnetic resonance imaging capturing changes in peak systolic velocity, 3-dimensional systolic flow profiles, and aortic wall shear stress that may predict a decreased risk of aortic dilation, a common complication in repeated Ross procedures.
Coordinated interactions between endothelial cells and macrophages in the islet microenvironment promote β cell regeneration (2021)

Endogenous β cell regeneration could alleviate diabetes, but proliferative stimuli within the islet microenvironment are incompletely understood. We previously found that β cell recovery following hypervascularization-induced β cell loss involves interactions with endothelial cells (ECs) and macrophages (MΦs). Here we show that proliferative ECs modulate MΦ infiltration and phenotype during β cell loss, and recruited MΦs are essential for β cell recovery. Furthermore, VEGFR2 inactivation in quiescent ECs accelerates islet vascular regression during β cell recovery and leads to increased β cell proliferation without changes in MΦ phenotype or number. Transcriptome analysis of β cells, ECs, and MΦs reveals that β cell proliferation coincides with elevated expression of extracellular matrix remodeling molecules and growth factors likely driving activation of proliferative signaling pathways in β cells. Collectively, these findings suggest a new β cell regeneration paradigm whereby coordinated interactions between intra-islet MΦs, ECs, and extracellular matrix mediate β cell self-renewal.
Atomic force microscope nanolithography of polymethylmethacrylate polymer (2007)

We describe a nanolithography process for a polymethylmethacrylate (PMMA) surface using scanning contact atomic force microscopy. Parallel furrows were scribed with a pyramidal silicon tip using the same scan mechanism as used to image samples. The PMMA was first electron beam irradiated using a scanning electron microscope and developed. The topography formed is reproducible and predictable. Material from the region where the tip scribes is moved to nearby regions, and aligned, elongated PMMA fragments are seen to decorate the valleys between furrows.
Effect of Polyols on Phosphorus-Containing Calcium Fluoride Deposition on Hydroxyapatite Surfaces (2018)

The present study investigated how polyols (sugar alcohols) affect the formation of calcium fluoride (CaF2) in the presence of fluoride on synthetic hydroxyapatite (HA) as a model of tooth enamel. HA plates were immersed in sugar alcohol solutions containing 10-60 (w/w)% xylitol, D-sorbitol, or glycerol and 1000 ppm fluoride at room temperature to analyze CaF2 formation on the surfaces. Only glycerol enhanced fluoride incorporation significantly and induced a nanoscale spherical deposition on the HA surface composed of calcium, fluoride, and a small amount of phosphorous according to energy-dispersive X-ray spectroscopic analysis. Overall results suggest that glycerol is capable of dissolving HA surfaces and enhancing phosphorous-containing CaF2 deposition, which can potentially prevent dental caries through the enhanced remineralization of enamel surfaces. Thus, fluoride may be involved in controlling the entire process of CaF2 deposition, dissolution, and remineralization of enamel.
Automatic Radar Target Classification A New Method Having Special Regard to Drones (2019)

The present algorithm stands for a novel method of automatic radar target classification. It specifically uses the recognition of target-typical characteristics and features of the spectrum and of the cepstrum, derived from the time-signal. In particular, the new technique allows reliable identification of the presence of drones.
New name for nursing federation (1982)

The Journal of Wound, Ostomy and Continence Nursing (JWOCN) is the premier publication for wound, ostomy and continence practice and research. The Journal’s mission is to publish current best evidence and original research to guide the delivery of expert health care. The WOCN Society is a professional nursing society which supports its members by promoting educational, clinical and research opportunities to advance the practice and guide the delivery of expert health care to individuals with wounds, ostomies and continence care needs.
Sulfur (2000)

The article contains sections titled: 1. Introduction 2. Historical Background 3. Properties 3.1. Physical State 3.2. Viscosity 3.3. Other Physical Properties 3.4. Chemical Properties 4. Raw Materials for Sulfur Production 4.1. Elemental Sulfur Resources 4.2. Sulfide Ores 4.3. Fossil Fuels 4.4. Sulfates 5. Production from Native Deposits 5.1. Flotation 5.2. Melting Out 5.3. Distillation 5.4. Flotation with Melting Out 5.5. The Frasch Process 5.5.1. Structure of “Fraschable” Sulfur Deposits 5.5.2. Outline of the Process 5.5.3. Sulfur Purity 5.5.4. Energy Consumption 5.5.5. The U.S. Frasch Industry 5.5.6. The Mexican Frasch Industry 6. The Claus Process 6.1. Occurrence of Hydrogen Sulfide 6.2. Hydrogen Sulfide Removal from Gases 6.3. History of the Claus Process 6.4. Chemistry of the Claus Process 6.5. Claus Plant Configurations 6.6. The Claus - Oxygen Process 6.7. Troubleshooting 7. Increased Sulfur Recovery 7.1. Selective Oxidation 7.1.1. The Superclaus Process 7.1.2. The Recycle Selectox Process 7.1.3. The Once-Through Selectox Process 7.2. Tail Gas Treatment 7.2.1. Sub-Dewpoint Processes 7.2.1.1. The Sulfreen, Hydrosulfreen, and Doxosulfreen Processes 7.2.1.2. The CBA Process 7.2.1.3. The MCRC Process 7.2.2. Processes Working on Reduced Tail Gases 7.2.2.1. The SCOT and Super-SCOT Processes 7.2.2.2. The Sulften Process 7.2.2.3. Solvents for Regenerative H2S Removal 7.2.2.4. The BSR/Selectox Process 7.2.2.5. The ELSE Process 7.2.3. Processes Working on Oxidized Tail Gas 7.2.3.1. The Wellman - Lord Process 7.2.3.2. The Clintox Process 7.3. Purification 7.3.1. Arsenic and Selenium Removal 7.3.2. Melting of Stretford Sulfur 7.3.3. Degassing of Claus Sulfur 8. Gas-Phase Reduction of Sulfur Dioxide 8.1. The Allied Chemicals Process 8.2. Other Processes with Gas as Reducing Agent 8.3. Processes with Coke or Coal as Reducing Agent 9. Commercial Grades and Forms of Sulfur 9.1. Quality 9.2. Commercial Forms for Bulk Transport 9.2.1. Dry Bulk Sulfur 9.2.2. Liquid Sulfur 9.2.3. Formed Sulfur 9.2.3.1. Slating 9.2.3.2. Wet Pelletizing (Water Prilling) 9.2.3.3. Air Prilling 9.2.3.4. Other Prilling Processes 9.2.3.5. Drum Granulation: The Procor GX Process 9.2.3.6. Spouted Bed Granulation 9.3. Special Forms and Grades 10. Uses 10.1. Agriculture 10.2. Construction Materials 10.3. Sulfur-Containing Batteries 10.4. Other Potential Uses
Investigation of the Excited State Structure of DCM via Ultrafast Electronic Pump/Vibrational Probe (2006)

Time resolved visible pump, infrared probe transient absorption measurements of the solutes 4-dicyanomethylene-2-methyl-6-(p-(dimethylamino)styryl)-4H-pyran (DCM) and its isotopomer DCM-d6 are employed to probe the dynamics of charge transfer state formation in dimethyl sulfoxide (DMSO) and acetonitrile (MeCN). We observe a two stage charge transfer (CT): the first step is an instrument-response-limited charge separation to the dicyanomethylene group, and the second involves a structural evolution of the dimethylamino group. Theoretical calculations and isotopic substitution indicate that the observed vibration is due to the dimethylamino group twisting out of plane, stabilizing the charge separation.
Decarboxylation of 6-Nitrobenzisoxazole-3-carboxylate in Polyampholyte Latexes (2000)

Polyampholyte latexes that contain 29/18 and 25/23 mol % of (styrylmethyl)trimethylammonium/methacrylate units and styrene cross-linked with divinylbenzene are colloidally stable in 4 M NaCl solution. As catalytic media in basic solutions 0.5 mg mL-1 of the polyampholyte latexes increase the rate of decarboxylation of 6-nitrobenzisoxazole-3-carboxylate (6-NBIC) 60−115 times, while the precursor polycation latexes increase the rate 540−670 times the rate in water alone. The latexes are active catalysts in 0.67 M NaCl solution, but activity decreases as the concentration of added NaCl increases. Analysis of rate constants at varied particle concentrations indicates that the fractions of the 6-NBIC anions bound to particles are similar in the two types of latexes and that the differences in activity are due primarily to larger intraparticle rate constants in the polycation latexes than in the polyampholytes.
Methodology assessment for the design and analysis of aero-engine short intakes (2020)

A key aspect for the design of an aero-engine intake is that it must operate at off-design conditions such as high incidence. For short intake design the interaction with the fan cannot be neglected. This work establishes how different modelling strategies can affect the intake design boundaries and the analysis of an aero-engine short-intake. Unsteady simulations showed high levels of total pressure and swirl fluctuations while steady computations showed some limitations whenever extreme operating conditions are modelled. The analysis of a short intake showed that the fan is able to extend the incidence limit to avoid separation by about 0.6°. Overall, this work proposes a robust methodology for short-intake analysis
Measurement Procedure to Investigate Ageing of Low-Power Voltage Transformers (2022)

The power network is experiencing a huge replacement of equipment. The legacy instrument transformers (ITs) are being substituted, at all voltage levels, with the new generation of low-power instrument transformers (LPITs). They feature various benefits compared to the previous generation. For example, they are smaller, they are easier to install, they have larger bandwidths, etc. compared to ITs. However, the recent introduction of LPITs results in a lack of information about several aspects concerning their behavior. For instance, there is almost no literature (or standard) dealing with the ageing of either current or voltage LPITs (LPCTs and LPVTs, respectively). Nevertheless, considering that once installed LPITs are rarely calibrated again before decades, information on their ageing is crucial. To this purpose, this work introduces a new test to investigate the ageing of LPITs. Furthermore, measurement procedures are described to evaluate the effect of ageing on their accuracy. Both the test and the measurement procedures are then experimentally validated on two off-the-shelf resistive LPVTs. From the results several conclusions can be drawn. First, the designed test is easy to implement and not expensive. Second, the accuracy of the device under test is clearly affected by the designed test. Third, the measurement procedure allows to estimate the thermal constant of a device and its in-field behavior.
Role of β-adrenoceptors in the anticonvulsant effect of propranolol on leptazol-induced convulsions in rats (1982)

Journal Article Role of β-adrenoceptors in the anticonvulsant effect of propranolol on leptazol-induced convulsions in rats Get access Jenny Papanicolaou, Jenny Papanicolaou Clinical Pharmacology and Therapeutics Unit, University of Melbourne, Austin Hospital, Heidelberg, Victoria, 3084, Australia Search for other works by this author on: Oxford Academic Google Scholar F J E Vajda, F J E Vajda Clinical Pharmacology and Therapeutics Unit, University of Melbourne, Austin Hospital, Heidelberg, Victoria, 3084, Australia Search for other works by this author on: Oxford Academic Google Scholar R J Summers, R J Summers Clinical Pharmacology and Therapeutics Unit, University of Melbourne, Austin Hospital, Heidelberg, Victoria, 3084, Australia Search for other works by this author on: Oxford Academic Google Scholar W J Louis W J Louis Clinical Pharmacology and Therapeutics Unit, University of Melbourne, Austin Hospital, Heidelberg, Victoria, 3084, Australia Search for other works by this author on: Oxford Academic Google Scholar Journal of Pharmacy and Pharmacology, Volume 34, Issue 2, February 1982, Pages 124–125, https://doi.org/10.1111/j.2042-7158.1982.tb04202.x Published: 12 April 2011 Article history Received: 18 May 1981 Published: 12 April 2011
Visual feedback for virtual grasping (2014)

We investigate visual feedback for virtual grasps, especially cues to improve behavior after real fingers enter a virtual object. To date, such visual cues have usually been developed in an ad-hoc manner, with minimal or no studies that can guide selection. Existing guidelines are based largely on other interaction types and provide inconsistent and potentially-misleading information when applied to grasping. We compare several different visual feedback types including those most commonly seen for virtual hand interaction and with some novel visual aspects. The visuals were tuned in a pilot study, and our main study evaluated results in terms of objective performance (finger penetration, release time, and precision) and subjective rankings. Performancewise, the most promising techniques all directly reveal penetrating hand configuration in some way. Subjectively, most techniques are better than simple interpenetrating visuals, with color changes being most promising. The results enable selection of the best cues based on the relevant tradeoffs. Results also provide a needed basis for more focused studies of specific visual cues and for better informing studies of multimodal feedback.
Smart probes for optical imaging of T cells and screening of anti-cancer immunotherapies (2023)

T cells are an essential part of the immune system with crucial roles in adaptive response and the maintenance of tissue homeostasis. Depending on their microenvironment, T cells can be differentiated into multiple states with distinct functions. This myriad of cellular activities have prompted the development of numerous smart probes, ranging from small molecule fluorophores to nanoconstructs with variable molecular architectures and fluorescence emission mechanisms. In this Tutorial Review, we summarize recent efforts in the design, synthesis and application of smart probes for imaging T cells in tumors and inflammation sites by targeting metabolic and enzymatic biomarkers as well as specific surface receptors. Finally, we briefly review current strategies for how smart probes are employed to monitor the response of T cells to anti-cancer immunotherapies. We hope that this Review may help chemists, biologists and immunologists to design the next generation of molecular imaging probes for T cells and anti-cancer immunotherapies.
Applications of Variable-Axial Fibre Designs in Lightweight Fibre Reinforced Polymers (2015)

A load dependent and curvilinear respectively variable-axial fibre design can notably enhance the strength and stiffness of lightweight components compared to fibre reinforced structures made of common multiaxial fibre textiles. At the Leibniz-Institut für Polymerforschung Dresden e. V. (IPF) special design strategies are in the focus of current studies. Two currently developed components made of carbon fibre reinforced plastics, a lightweight three-legged stool and a lightweight recurve bow riser, are described within this paper.
Femtosecond Fluorescence Spectra of NADH in Solution: Ultrafast Solvation Dynamics (2020)

The ultrafast solvation dynamics of reduced nicotinamide adenine dinucleotide (NADH) free in solution has been investigated, using both a femtosecond upconversion spectrophotofluorometer and a picosecond time-correlated single-photon counting (TCSPC) apparatus. The familiar time constant of solvent relaxation originating in "bulk water" was found to be ∼1.4 ps, revealing ultrafast solvent reorientation upon excitation. We also found a slower spectral relaxation process with an apparent time of 27 ps, suggesting there could either be dissociable "biological water" hydration sites on the surface of NADH or internal dielectric rearrangements of the flexible solvated molecule on that timescale. In contrast, the femtosecond fluorescence anisotropy measurement revealed that rotational diffusion happened on two different timescales (3.6 ps (local) and 141 ps (tumbling)); thus, any dielectric rearrangement scenario for the 27 ps relaxation must occur without significant chromophore oscillator rotation. The coexistence of quasi-static self quenching (QSSQ) with the slower relaxation is also discussed.
Expression of swine MHC class II genes in a cynomolgus monkey: Retro virus‐mediated gene therapy in a preclinical transplantation model (1997)

Abstract: Immune reactivity against products of the major histocompatibility complex (MHC) is the major barrier to allotransplantation. Conversely, sharing of MHC class II antigens I appears to be of overwhelming importance in permitting the induction of immune tolerance to vascularized organ allografts, as demonstrated previously in miniature swine. Class II antigen has also been shown to be recognized predominantly in human anti‐pig xenoreactions in vitro. To achieve tolerance in the xenogeneic pig‐to‐primate model, we are therefore investigating an approach involving retrovirus‐mediated gene therapy to transfer swine MHC (SLA) class II genes into primate primitive hematopoietic stem cells, so that swine MHC class II antigens may participate in the education of the recipient's newly developing T cell repertoire. We report here the in vitro and in vivo use of a recombinant retrovirus containing a polycistronic retroviral vector which carries swine MHC class II DRA and DRB cDNA sequences to transduce CD34+ bone marrow cells (BMC) from a cynomolgus monkey. Transduction efficiency was assessed by reverse transcriptase‐polymerase chain reaction analysis of the colony‐forming unit progenitor colonies grown in the absence of gentamycin; 55% and 24% of the progenitor colonies were determined to express the retroviral transcript at 1 week and 3 weeks post‐transduction, respectively. These in vitro studies have been extended to the transplantation of retrovirally transduced autologous stem cells into a cynomolgus monkey prepared with a non‐myeloablative conditioning regimen. Prolonged expression of SLA‐DR transcripts in monkey peripheral blood mononuclear cells (PBMC) has been documented over a 56‐week period after transplantation of retrovirus‐transduced bone marrow cells. However, we could not detect any protein expression by FACS analysis on the surface of primate PBMC or bone marrow, using a porcine SLA‐DR‐specific antibody. Engraftment of hematopoietic cells with the transduced genes was further detected in the progenitor colonies grown from the bone marrow cells harvested at 4 weeks and 25 weeks after bone marrow transplantation. Our results thus document that long‐term engraftment of retrovirally transduced hematopoietic cells can be achieved in a primate model using a non‐myeloablative preparative I regimen.
Real-time Plane-Sweep with local strategy (2006)

Recent research in computer vision has made significant progress in the reconstruction of depth information\nfrom two-dimensional images. A new challenge is to extend these techniques to video images. Given a small set\nof calibrated video cameras, our goal is to render on-line dynamic scenes in real-time from new viewpoints. This\npaper presents an image-based rendering system using photogrametric constraints without any knowledge of the\ngeometry of the scene. Our approach follows a plane-sweep algorithm extended by a local dynamic scoring that\nhandles occlusions. In addition, we present an optimization of our method for stereoscopic rendering which\ncomputes the second image at low cost. Our method achieves real-time framerate on consumer graphic hardware\nthanks to fragment shaders.