gebhart/patrepeval huggingface dataset:
Colloidal Properties of Nanoerythrosomes Derived from Bovine Red Blood Cells (2015)

Liposomes are nanoscale containers that are typically synthesized from lipids using a high-shear process such as extrusion or sonication. While liposomes are extensively used in drug delivery, they do suffer from certain problems including limited colloidal stability and short circulation times in the body. As an alternative to liposomes, we explore a class of container structures derived from erythrocytes (red blood cells). The procedure involves emptying the inner contents of these cells (specifically hemoglobin) and resuspending the empty structures in buffer, followed by sonication. The resulting structures are termed nanoerythrosomes (NERs), i.e., they are membrane-covered nanoscale containers, much like liposomes. Cryo-transmission electron microscopy (cryo-TEM) and small-angle neutron scattering (SANS) are employed for the first time to study these NERs. The results reveal that the NERs are discrete spheres (∼110 nm diameter) with a unilamellar membrane of thickness ∼4.5 nm. Remarkably, the biconcave disc-like shape of erythrocytes is also exhibited by the NERs under hypertonic conditions. Moreover, unlike typical liposomes, NERs show excellent colloidal stability in both buffer as well as in serum at room temperature, and are also able to withstand freeze-thaw cycling. We have explored the potential for using NERs as colloidal vehicles for targeted delivery. Much like conventional liposomes, NER membranes can be decorated with fluorescent or other markers, solutes can be encapsulated in the cores of the NERs, and NERs can be targeted to specifically bind to mammalian cells. Our study shows that NERs are a promising and versatile class of nanostructures. NERs that are harvested from a patient's own blood and reconfigured for nanomedicine can potentially offer several benefits including biocompatibility, minimization of immune response, and extended circulation time in the body.
A 34dB SNDR instantaneously-companding baseband SC filter for 802.11a/g WLAN receivers (2010)

In wireless receivers, large dynamic range (DR) input signals necessitate additional power consumption in baseband active filters. A 5 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> order Chebychev, ladder type, companding SC low-pass filter is described in this paper which provides gain switching based on the instantaneous value of the signal to handle peak-to-average-power ratio (PAPR), thereby reducing power dissipation for a given DR. Companding compresses the high DR input signal, processes it in a lower DR system (the filter in our case) and then expands the signal at the output [1]. Companding is an alternative to AGC, which sets the filter internal gains during the preamble/midamble so that the signal level in the filter is optimal to provide the minimum SNDR required by the specifications. The IEEE 802.11a/g WLAN receiver [2] presents 2 limitations to the use of AGC. First, the standard puts a stringent AGC settling time requirement (≪5.6µs). Since the filter needs extra settling time, AGC is implemented after the filter and before the ADC in the receiver baseband. Secondly, the high PAPR of OFDM signals requires headroom of at least 12dB in the DR of the filter. While dynamic impedance and gain scaling techniques have been proposed in the past as an alternative to AGC [3], this work addresses the PAPR problem.
Influence of Low-Magnitude High-Frequency Vibration on Bone Cells and Bone Regeneration (2020)

Bone is a mechanosensitive tissue for which mechanical stimuli are crucial in maintaining its structure and function. Bone cells react to their biomechanical environment by activating molecular signaling pathways, which regulate their proliferation, differentiation, and matrix production. Bone implants influence the mechanical conditions in the adjacent bone tissue. Optimizing their mechanical properties can support bone regeneration. Furthermore, external biomechanical stimulation can be applied to improve implant osseointegration and accelerate bone regeneration. One promising anabolic therapy is vertical whole-body low-magnitude high-frequency vibration (LMHFV). This form of vibration is currently extensively investigated to serve as an easy-to-apply, cost-effective, and efficient treatment for bone disorders and regeneration. This review aims to provide an overview of LMHFV effects on bone cells <i>in vitro</i> and on implant integration and bone fracture healing <i>in vivo</i>. In particular, we review the current knowledge on cellular signaling pathways which are influenced by LMHFV within bone tissue. Most of the <i>in vitro</i> experiments showed that LMHFV is able to enhance mesenchymal stem cell (MSC) and osteoblast proliferation. Furthermore, osteogenic differentiation of MSCs and osteoblasts was shown to be accelerated by LMHFV, whereas osteoclastogenic differentiation was inhibited. Furthermore, LMHFV increased bone regeneration during osteoporotic fracture healing and osseointegration of orthopedic implants. Important mechanosensitive pathways mediating the effects of LMHFV might be the Wnt/beta-catenin signaling pathway, the estrogen receptor (ER) signaling pathway, and cytoskeletal remodeling.
Sichtbare Flexionsmorphologie im Englischen und Deutschen (2017)

The aim of this paper is twofold. The first is to develop a theoretical framework for the relation between spelling and morphology. To this end, morphological spellings are divided into stem and affix spellings. A second orthogonal classification divides both according to the respective perspective, reading and writing. The second aim is to show that this framework can be operationalized. To do so, one subtype of morphological spellings, the reading of inflectional affixes, is empirically investigated in both English and German. The results show that in English, inflectional affixes are often spelled in a unique way, with little to no homographic word endings of monomorphemic words. In German, this is not the case: Here we find widespread homography between affix spellings and word endings.
Marine Operation Windows Offshore Norway (2016)

A study of the wave conditions in the North Sea, the Norwegian Sea and the Barents Sea is presented in this paper. For each region, one reference location for which there are buoy measurements is selected. For the selected locations, WAM10 hindcast data are obtained from the Norwegian Meteorological Institute (MET Norway). The hindcast data for each location cover the period from 1957 to 2014. First, the hindcast datasets were validated against available buoy measurements — both for extreme value predictions and for application of hindcast data for planning of marine operations. The validation was carried out considering the winter season and the summer season separately. For each season, the datasets for two consecutive months were used. A comparison of the time-series of the hindcast datasets against the buoy measurements showed that the hindcast datasets compared relatively well with the buoy measurements. However, a comparison of the statistical parameters of the hindcast datasets against the buoy measurements showed that the hindcast datasets are slightly conservative in the estimate of the significant wave height for the Barents Sea and the Norwegian Sea. Overall, the data compared well, and the hindcast datasets are therefore considered in the following analysis. Hindcast data from these 57 years show that the wave conditions in the selected Norwegian Sea location is harsher than the wave conditions in both the North Sea and the Barents Sea locations. This is in agreement with the general expected spatial trend in the wave climate on the Norwegian Continental Shelf (NCS). It was also observed that the wave conditions in the selected Barents Sea location are harsher than the wave conditions in the North Sea. These findings are also reflected in the NORSOK N-003 standard on “Actions and Action effects” (NORSOK, 2015). The weather windows for weather-sensitive marine operations, that is, operations with operational reference period not exceeding 72 hours, were established from the hindcast dataset for each of the locations. It was observed that the Norwegian Sea has shorter weather windows, especially in the winter seasons, compared to both the Barents Sea and the North Sea. It was expected that the operational windows would be shorter in the winter seasons in the Barents Sea, due to the occurrence of polar lows. However, the polar lows are few and cause more concern related to forecasting of the weather conditions to start actual marine operations. Generally, the month with the highest probability of weather windows exceeding 72 hours was found to be July for all three locations.
ChemInform Abstract: Synthesis of 3‐Hetarylcoumarins from 3‐Acetylcoumarins. (1986)

Abstract Unter Vilsmeier‐ Bedingungcn bilden sich aus den Cumarinderivaten (Ia) bis (Ic) die Pyrazole (IIa)‐(IIc), während in Polyphosphorsäure die Indole (IIIa)‐(IIIc) entstehen.
A Release-Aware Bug Triaging Method Considering Developers' Bug-Fixing Loads (2020)

This paper proposes a release-aware bug triaging method that aims to increase the number of bugs that developers can fix by the next release date during open-source software development. A variety of methods have been proposed for recommending appropriate developers for particular bug-fixing tasks, but since these approaches only consider the developers' ability to fix the bug, they tend to assign many of the bugs to a small number of the project's developers. Since projects generally have a release schedule, even excellent developers cannot fix all the bugs that are assigned to them by the existing methods. The proposed method places an upper limit on the number of tasks which are assigned to each developer during a given period, in addition to considering the ability of developers. Our method regards the bug assignment problem as a multiple knapsack problem, finding the best combination of bugs and developers. The best combination is one that maximizes the efficiency of the project, while meeting the constraint where it can only assign as many bugs as the developers can fix during a given period. We conduct the case study, applying our method to bug reports from Mozilla Firefox, Eclipse Platform and GNU compiler collection (GCC). We find that our method has the following properties: (1) it can prevent the bug-fixing load from being concentrated on a small number of developers; (2) compared with the existing methods, the proposed method can assign a more appropriate amount of bugs that each developer can fix by the next release date; (3) it can reduce the time taken to fix bugs by 35%-41%, compared with manual bug triaging;
Laser Beam Sampling Gratings With Low Absorption And Specified Efficiency (1987)

A diffraction grating sampler to detect wavefront and phase emitted by a high energy laser at 10.6 µm was calculated and manufactured. The grating extracted a small fraction of the incident energy and reflected the rest like a mirror. The portion of the beam sampled was diffracted into the 1st order to a detector that operated a servo control of the wavefront phase and power.
Methane-trapping metal–organic frameworks with an aliphatic ligand for efficient CH4/N2 separation (2019)

Stable MOFs with an aliphatic ligand exhibit an excellent performance for the separation of CH<sub>4</sub>/N<sub>2</sub> mixture.
2 CORRELATION BETWEEN OXYGEN RESPIRATION RATES AND MORPHOLOGY, SEX, DIAMETER AND DEVELOPMENTAL STAGE OF SINGLE BOVINE IVP-EMBRYOS (2004)

A simple, non-invasive, rapid and sensitive oxygen microsensor system was developed to investigate correlations between oxygen respiration rates of individual bovine embryos and their morphology, sex, diameter and developmental stage. Bovine IVP-embryos (n = 78; Holm et al. Theriogenology 52, 683–700) were analysed around the 8-cell stage (Day 3; n = 18) and at various blastocyst stages (Day 7; n = 60). Each embryo was morphologically evaluated, its outer diameter measured and was then loaded into a glass tube (i.d. 0.68 mm, length 3 mm). After 1 h, oxygen concentration gradients generated by the embryo’s respiration were measured over app. 8 min with an oxygen microelectrode (www.unisense.com). Five embryos were measured in one round together with an empty tube as control. The procedure was repeated twice for each embryo with app. 1 h interval. Individual respiration rates in nL O2/embryo/h (nL/h) were calculated from these gradients. The measurements were performed at 38.5°C under constant flow of humidified 5% CO2 in air (app. 19% O2). After this, 64 embryos (14 Day 3; 50 Day 7) were lysed for sex diagnosis by PCR. Values are given as mean ± SD. The sensitivity of the oxygen measurement system was high (controls: 0.034 ± 0.035 nL/h, n = 15) and its repeatability from 1st to 2nd measurement was 99.7 ± 9.8% (n = 71). The average embryo respiration rate was 0.39 ± 0.05 nLl/h on Day 3 (n = 18) and 1.31 ± 0.52 nLl/h on Day 7 (n = 60). For Day 7 embryos, the respiration rates varied according to their morphological quality, being 1.87 ± 0.46a (n = 18), 1.17 ± 0.32b (n = 23), 0.95 ± 0.27b,c (n = 14) and 0.72 ± 0.24c (n = 4) nL/h for quality 1, 2, 3, and 4 embryos, respectively (Proc Mixed,a,b,c: P &amp;lt; 0.05; values with different superscripts differ significantly). The sex ratio (male:female) was 9:5 (Day 3) and 32:18 (Day 7), and on Day 7 this ratio varied between qualities: 11:2, 12:8, 8:4, and 1:3 for quality 1, 2, 3, and 4, respectively. The average respiration rate on day 3 was the same for males and females, as it was on day 7 (1.22 ± 0.43 nL/h (females) and 1.31 ± 0.58 nL/h (males), P &amp;gt; 0.05). There was a correlation between embryo diameter and respiration rate (r2 = 0.65, n = 74), which was even stronger for Day 7 male embryos (r2 = 0.72, n = 32). In conclusion, a highly reliable, repeatable and sensitive system was established for measuring respiration rates in single bovine embryos, even at early developmental stages. The respiration rate was lower on day 3 compared to Day 7 embryos, and it was correlated with the morphological embryo quality on Day 7. Oxygen consumption could be a valuable supplementary indicator of embryo viability, especially in difficult evaluations (e.g. quality 2 and 3 after IVP). It remains to be demonstrated if such measurements can also reveal quality differences already at Day 3, which would be of interest in, e.g. the human field. ASL is supported by FCT, Portugal.
Abstract 3153: Transcriptome differences in tyrosine kinase inhibitor-resistant clones of EGFR-mutant lung cancer using a new microfluidic assay for concurrent single-cell RNA and targeted DNA sequencing (2017)

Abstract Resistance to therapy is one of the major causes of cancer-associated death. While a number of mechanisms for the development of resistance to epidermal growth factor receptor, EGFR-targeting drugs in lung cancer have been described, most genomic and transcriptomic studies have focused on analyzing bulk samples that can only provide an average measurement over the entire mixture of cell populations of a tumor. With this approach, it is difficult to resolve cell-to-cell variability of drug resistance within a heterogeneous tumor. In order to accurately describe and eventually delineate the underlying causes of cancer progression, it requires the analysis of single cells on both, the transcriptomic and the genomic level. Only the co-detection of mutations and expression features in individual cells allows to define the connection between the two. We therefore aimed to establish a new methodology for concurrent evaluation of transcriptomic and genomic features within the same single cell on the Fluidigm C1 system. We applied this protocol to an EGFR-mutant lung cancer cell line, PC9, that has developed resistance to tyrosine kinase inhibitor, TKI treatment after prolonged exposure to sublethal doses of Gefitinib. We amplified cDNA and selected genomic EGFR regions of the TKI responsive and resistant PC9 clones from a total of ≈300 single cells. We used our in-house single cell’s analysis pipeline for parallel processing of transcriptome and variants detection. In addition, a novel algorithm, NODES was used to normalize the single cell RNAseq data and detect differentially expressed genes. The differential transcriptome and emergence of the EGFR T790M resistance mutation in relation to the TKI response were evaluated. We observed up-regulation of receptor tyrosine kinase AXL in the resistant cell lines. This observation is in agreement with previous findings that AXL is the key player that promotes TKI resistance in lung cancer. In addition, we found up-regulation of other genes that have not been previously described. Cumulatively, this method allows us to dissect the underpinnings of the TKI resistance mechanism and enables us to identify biomarker for specific cellular features that are connected with resistance and thereby with lung cancer patient’s response to TKI treatment. Citation Format: Say Li Kong, Huipeng Li, Dave Ruff, Joyce An Yi Tai, Elise T. Courtois, Huay Mei Poh, Dawn Pingxi Lau, Audrey Ann Liew, Gek San Tan, Tony Kiat Hon Lim, Daniel Shao Weng Tan, Shyam Prabhakar, Axel M. Hillmer. Transcriptome differences in tyrosine kinase inhibitor-resistant clones of EGFR-mutant lung cancer using a new microfluidic assay for concurrent single-cell RNA and targeted DNA sequencing [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3153. doi:10.1158/1538-7445.AM2017-3153
Joining Hands: Using Consortia to Efficiently Create Easily Accessible International Experiences for Engineering Students (2020)

Abstract Top 3 Topics abstract fits into from “Call for Papers:”Authors: Sabeen Altaf, IIE; Randy Collins, Clemson Univeristy, Eck Doerry, Northern Arizona University;Larry Shuman, University of Pittsburgh1. Needs, Opportunities, and Challenges for Global Engineering Education2. Programs for engineering students to gain international experience3. International Collaborations, Experiences, Partnerships, Service LearningPaper Title: Creating innovative study abroad opportunities for engineering students through Consortia.The paper addresses how two consortia-based programs - Global Engineering Education Exchange(Global E3), administered by the Institute of International Education (IIE), and Germany’s UAS7(University of Applied Sciences 7) consortium, were created to address both real and perceivedobstacles that engineering students face when considering a study abroad experience, including issuesrelated to cost, credit, and housing. The paper will present an overview of why these two programswere created, document how they have grown, their ability to respond to current needs in engineeringeducation, remaining challenges for each, and then draw some conclusions about the future of the twoprograms. We will discuss the advantages to both students and institutions of consortia programs andsome of the concerns. We will also address how programs such as Global E3 and the UAS7 modelattempt to navigate various higher education systems, responding to both old and new challenges increating a “globally competent” engineer. We will also address how consortia programs can help fosternew approaches to study abroad. Finally, the paper will describe how the respective consortium-basedprograms have helped internationalize the field of engineering education by creating strategicpartnerships around the world and using the respective programs as a platform for long-termcollaborations amongst institutions that go beyond student mobility.
Placement-driven technology mapping for LUT-based FPGAs (2003)

In this paper, we study the problem of placement-driven technology mapping for table-lookup based FPGA architectures to optimize circuit performance. Early work on technology mapping for FPGAs such as Chortle-d[14] and Flowmap[3] aim to optimize the depth of the mapped solution without consideration of interconnect delay. Later works such as Flowmap-d[7], Bias-Clus[4] and EdgeMap consider interconnect delays during mapping, but do not take into consideration the effects of their mapping solution on the final placement. Our work focuses on the interaction between the mapping and placement stages. First, the interconnect delay information is estimated from the placement, and used during the labeling process. A placement-based mapping solution which considers both global cell congestion and local cell congestion is then developed. Finally, a legalization step and detailed placement is performed to realize the design. We have implemented our algorithm in a LUT based FPGA technology mapping package named PDM (Placement-Driven Mapping) and tested the implementation on a set of MCNC benchmarks. We use the tool VPR[1][2] for placement and routing of the mapped netlist. Experimental results show the longest path delay on a set of large MCNC benchmarks decreased by 12.3% on the average.
Application of maximal ratio combining for iterative multiuser decoding (2004)

In this paper we apply the concept of recursive maximal ratio combining over iterations in an iterative multiuser decoder. The principle can be applied to any multiuser estimator that does not exploit information across iterations. The estimates over all iterations are combined based on MRC, such that the resulting SNR is maximized.
3D single-molecule tracking using one- and two-photon excitation microscopy (2014)

Three dimensional single-molecule tracking (3D-SMT) has revolutionized the way we study fundamental cellular processes. By analyzing the spatial trajectories of individual molecules (e.g. a receptor or a signaling molecule) in 3D space, one can discern the internalization or transport dynamics of these molecules, study the heterogeneity of subcellular structures, and elucidate the complex spatiotemporal regulation mechanisms. Sub-diffraction localization precision, sub-millisecond temporal resolution and tens-of-seconds observation period are the benchmarks of current 3D-SMT techniques. We have recently built two molecular tracking systems in our labs. The first system is a previously reported confocal tracking system, which we denote as the 1P-1E-4D (one-photon excitation, one excitation beam, and four fiber-coupled detectors) system. The second system is a whole new design that is based on two-photon excitation, which we denote as the 2P-4E-1D (two-photon excitation, four excitation beams, and only one detector) system. Here we compare these two systems based on Monte Carlo simulation of tracking a diffusing fluorescent molecule. Through our simulation, we have characterized the limitation of individual systems and optimized the system parameters such as magnification, z-plane separation, and feedback gains.