gebhart/patrepeval huggingface dataset:
Epigraph Hemagglutinin Vaccine Induces Broad Cross-reactive Immunity Against Swine H3 Influenza Virus (2020)

Abstract Swine influenza virus (SIV) is a significant burden on the pork industry and threat to human health due to its zoonotic potential. Here, we utilized epigraph, a computational algorithm, to design a universal swine H3 (swH3) influenza vaccine. The epigraph hemagglutinin proteins were delivered using an Adenovirus type 5 vector and compared to a wild type hemagglutinin and the commercial inactivated vaccine, FluSure. In mice, epigraph vaccination led to significant cross-reactive antibody and T-cell responses against a diverse panel of swH3 isolates. Epigraph vaccination also reduced weight loss and lung viral titers in mice after challenge with three divergent swH3 viruses. Vaccination studies in swine, the target species for this vaccine, showed stronger levels of cross-reactive antibodies and T-cell responses after immunization with epigraph vaccine compared to the wild type and FluSure vaccines. In both murine and swine models, epigraph vaccination showed superior cross-reactive immunity that should be further investigated as a universal swH3 vaccine.
BMP-2 induces EMT and breast cancer stemness through Rb and CD44 (2017)

Bone morphogenetic protein 2 (BMP-2) has been reported to facilitate epithelial-to-mesenchymal transition (EMT) and bone metastasis in breast cancer xenograft models. To investigate the role of BMP-2 in the development of breast cancer stem cells (BCSCs), and to further elucidate the mechanisms underlying its influence on breast cancer metastasis, we conducted a comprehensive molecular study using breast cancer cell lines and clinical samples. Our results showed that downregulation of Rb by BMP-2 was associated with ubiquitin-mediated degradation activated by phosphorylation of Rb via the PI3K/AKT signal pathway. In addition, the Smad signaling pathways are implicated in upregulation of CD44 protein expression by BMP-2. It was suggested that cross-talk exists between Rb and CD44 signaling pathways, as recombinant human BMP-2 (rhBMP-2) was found to regulate CD44 expression partly through Rb signals. In clinical tissues, BMP-2 was positively and negatively correlated with CD44 and Rb expression, respectively. Based on the <i>in vitro</i> and <i>in vivo</i> results, we have established an integrated mechanism by which rhBMP-2 induces EMT and stemness of breast cancer cells via the Rb and CD44 signaling pathways, which then contribute to breast cancer metastasis. These findings may be helpful for developing new strategies for the treatment and prognosis of advanced breast cancer.
Tumor filodes de mama. Hallazgos en imagen (2012)

Poster: SERAM 2012 / S-1150 / Tumor filodes de mama. Hallazgos en imagen by: Barcelo Obregon1, N. Viejo Mira1, J. C. Vilanova2, J. Albanell Mira1, F. Castaner Corretger1, A. Gallardo1; 1Girona/ES, 2Gerona/ES
Abstract 329: Identification of the breakpoints of two chromosome translocations involving chromosomes 4 and 7 observed in two murine mammary carcinomas (2010)

Abstract The translocation between chromosomes 4 and 7, T(4;7), was observed in two different mammary carcinomas from the medroxyprogesterone acetate (MPA) mouse breast cancer model which have acquired a hormone independent (HI) phenotype (C7-2-HI and C4-HI). We had developed a cell line from the C7-2-HI tumor, the MC7-L2A cells, which retain the chromosome translocation T(4;7), and provide an easy tool to obtain metaphases. G-banding analysis showed that the breakpoint on chromosome 4 is located on the band 4E in the C7-2-HI tumor, whereas in the C4-HI tumor, it is located on the region 4C4-4D3. However, the breakpoint on chromosome 7 is located near the centromere in both tumors. Further experiments using fluorescence in situ hybridization (FISH) in MC7-L2A cells have shown that the breakpoint of the translocation involved the chromosome bands 4E2 and 7A1-A2. The aim of this project is to elucidate whether these breakpoints are involving new gene rearrangements that might participate in the acquisition of hormone independency. In this study we focused in determining if the break in chromosome 7 is located on the same band in both HI tumors. A detailed analysis in MC7-L2A cells using FISH with different DNA probes, spanning the region 7A1-7A2, showed that the breakpoint is located on chromosome 7A1 and not on band 7A2. However, a different picture was observed in metaphases obtained from C4-HI tumor cells, in which the probes corresponding to region 7A1-7B1 hybridized only to normal chromosome 7, but not to the translocation T(4;7). These results indicate that the breakpoints on chromosomes 4 and 7, although involving the same chromosomes, are located on different regions in the T(4;7) translocations, suggesting that they may be random breakpoints. Even though we could not associate this breakpoint with the hormone-independent phenotype, the identification of the sites of chromosome breaks may be relevant to understand the biology of each HI tumor. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 329.
Four-wave mixing using partially coherent fields in systems with spatial correlations (1986)

A microscopic correlation function expression for the signal in four-wave mixing is developed. Both stationary and transient (i.e., photon-echo) experiments with coherent or incoherent radiation fields are considered. In general, these observables are described by an eight-point correlation function of the dipole operator involving two particles, and the signal cannot be written as an amplitude squared. Conditions under which the correlation function may be factorized into a product of two four-point, single-particle correlation functions are specified. In this case the conventional approach, based on calculating a nonlinear polarization and substituting it as a source in Maxwell's equations, is justified. However, in the presence of long-range spatial correlations in the sample (e.g., near critical points) the conventional formulation breaks down and the present theory should be used.
Electrochromic properties of polythiophene and poly(3-methylthiophene). (1987)

ITO電極上に電解重合法で被覆したポリチオフェンならびにポリ3-メチルチオフェン薄膜のエレクトロクロミック特性について三電極方式で調べた. その結果, 酸化反応における応答速度に関し, ポリ3-メチルチオフェンの応答速度はポリチオフェンよりも高いことが, 吸光度及び通電電荷量から明らかとなった. 一方, サイクリックボルタンメトリーから, ポリ3-メチルチオフェンの酸化還元電位はポリチオフェンよりも高い (貴である) こと. また, ポリ3-メチルチオフェンの酸化反応におけるピーク電流は電位走査速度の0.7～0.9次に比例することがわかった. これらの結果は両ポリマーがその電子状態及び高次構造において大きく異なることを示唆している.
Study of Turn-to-Turn Electrical Breakdown for Superconducting Fault Current Limiter Applications (2019)

The rational insulation design of a resistive superconducting fault current limiter (r-SCFCL) requires data gathered from experimental setups representative of the final apparatus. Therefore, an experimental study was performed to characterize the electrical breakdown (BD) of liquid nitrogen in the particular conditions of a quenching superconducting device. To reproduce the electrical stress occurring within a real r-SCFCL winding, BD voltages with a grounded tape electrode were investigated with different polarities and pressures, with and without transient heating on the tape electrode. Pictures obtained with a high-speed camera and breakdown delay measurements allow to further characterize breakdown mechanisms.
Low-loss 25.3km few-mode ring-core fibre for mode-division multiplexed transmission (2016)

We report the design, fabrication and characterisation of a few-mode ring-core fibre supporting 4 mode groups. The low loss (~0.3dB/km) and length (25.3km) are both records for a ring-core fibre.
Allostery in Recombinant Soluble Guanylyl Cyclase from Manduca sexta (2008)

Soluble guanylyl/guanylate cyclase (sGC), the primary biological receptor for nitric oxide, is required for proper development and health in all animals. We have expressed heterodimeric full-length and N-terminal fragments of Manduca sexta sGC in Escherichia coli, the first time this has been accomplished for any sGC, and have performed the first functional analyses of an insect sGC. Manduca sGC behaves much like its mammalian counterparts, displaying a 170-fold stimulation by NO and sensitivity to compound YC-1. YC-1 reduces the NO and CO off-rates for the approximately 100-kDa N-terminal heterodimeric fragment and increases the CO affinity by approximately 50-fold to 1.7 microm. Binding of NO leads to a transient six-coordinate intermediate, followed by release of the proximal histidine to yield a five-coordinate nitrosyl complex (k(6-5) = 12.8 s(-1)). The conversion rate is insensitive to nucleotides, YC-1, and changes in NO concentration up to approximately 30 microm. NO release is biphasic in the absence of YC-1 (k(off1) = 0.10 s(-1) and k(off2) = 0.0015 s(-1)); binding of YC-1 eliminates the fast phase but has little effect on the slower phase. Our data are consistent with a model for allosteric activation in which sGC undergoes a simple switch between two conformations, with an open or a closed heme pocket, integrating the influence of numerous effectors to give the final catalytic rate. Importantly, YC-1 binding occurs in the N-terminal two-thirds of the protein. Homology modeling and mutagenesis experiments suggest the presence of an H-NOX domain in the alpha subunit with importance for heme binding.
σ B Inhibits Poly- N -Acetylglucosamine Exopolysaccharide Synthesis and Biofilm Formation in Staphylococcus aureus (2019)

<i>Staphylococcus aureus</i> clinical strains are able to produce at least two distinct types of biofilm matrixes: biofilm matrixes made of the polysaccharide intercellular adhesin (PIA) or poly-<i>N</i>-acetylglucosamine (PNAG), whose synthesis is mediated by the <i>icaADBC</i> locus, and biofilm matrixes built of proteins (polysaccharide independent). σ<sup>B</sup> is a conserved alternative sigma factor that regulates the expression of more than 100 genes in response to changes in environmental conditions. While numerous studies agree that σ<sup>B</sup> is required for polysaccharide-independent biofilms, controversy persists over the role of σ<sup>B</sup> in the regulation of PIA/PNAG-dependent biofilm development. Here, we show that genetically unrelated <i>S. aureus</i> σ<sup>B</sup>-deficient strains produced stronger biofilms under both static and flow conditions and accumulated higher levels of PIA/PNAG exopolysaccharide than their corresponding wild-type strains. The increased accumulation of PIA/PNAG in the σ<sup>B</sup> mutants correlated with a greater accumulation of the IcaC protein showed that it was not due to adjustments in <i>icaADBC</i> operon transcription and/or <i>icaADBC</i> mRNA stability. Overall, our results reveal that in the presence of active σ<sup>B</sup>, the turnover of Ica proteins is accelerated, reducing the synthesis of PIA/PNAG exopolysaccharide and consequently the PIA/PNAG-dependent biofilm formation capacity.<b>IMPORTANCE</b> Due to its multifaceted lifestyle, <i>Staphylococcus aureus</i> needs a complex regulatory network to connect environmental signals with cellular physiology. One particular transcription factor, named σ<sup>B</sup> (SigB), is involved in the general stress response and the expression of virulence factors. For many years, great confusion has existed about the role of σ<sup>B</sup> in the regulation of the biofilm lifestyle in <i>S. aureus</i> Our study demonstrated that σ<sup>B</sup> is not necessary for exopolysaccharide-dependent biofilms and, even more, that <i>S. aureus</i> produces stronger biofilms in the absence of σ<sup>B</sup> The increased accumulation of exopolysaccharide correlates with higher stability of the proteins responsible for its synthesis. The present findings reveal an additional regulatory layer to control biofilm exopolysaccharide synthesis under stress conditions.
Control of interface abruptness of polar MgZnO/ZnO quantum wells grown by pulsed laser deposition (2010)

A strong quantum confined Stark effect (QCSE) was observed in wedge shaped MgZnO/ZnO quantum wells (QWs) grown by pulsed laser deposition. A reduced laser fluence of 1.8 J/cm2 was used. Reference samples grown at higher standard fluence 2.4 J/cm2 showed only a negligible QCSE. Using off-axis deposition without substrate rotation, a constant composition of the barriers was maintained while varying the well width in a wedge shaped QW. A redshift of the QW luminescence with increasing QW thickness up to 230 meV below the ZnO emission was found, accompanied by an increase in the exciton lifetime from 0.3 ns up to 4.2 μs.
Metal‐Catalyzed Enantioselective Allylation in Asymmetric Synthesis (2007)

Metal-catalyzed enantioselective allylation, which involves the substitution of allylic metal intermediates with a diverse range of different nucleophiles or S(N)2'-type allylic substitution, leads to the formation of C-H, -C, -O, -N, -S, and other bonds with very high levels of asymmetric induction. The reaction may tolerate a broad range of functional groups and has been applied successfully to the synthesis of many natural products and new chiral compounds.
Resource Allocation Schemes For Target Localization In Distributed Multiple Radar Architectures (2010)

Publication in the conference proceedings of EUSIPCO, Aalborg, Denmark, 2010
Data from Ultrasmall Nanoparticle Delivery of Doxorubicin Improves Therapeutic Index for High-Grade Glioma (2023)

&lt;div&gt;AbstractPurpose:&lt;p&gt;Despite dramatic growth in the number of small-molecule drugs developed to treat solid tumors, durable therapeutic options to control primary central nervous system malignancies are relatively scarce. Chemotherapeutic agents that appear biologically potent in model systems have often been found to be marginally effective at best when given systemically in clinical trials. This work presents for the first time an &lt;i&gt;ultrasmall&lt;/i&gt; (&lt;8 nm) multimodal core-shell silica nanoparticle, Cornell prime dots (or C′ dots), for the efficacious treatment of high-grade gliomas.&lt;/p&gt;Experimental Design:&lt;p&gt;This work presents first-in-kind renally clearable &lt;i&gt;ultrasmall&lt;/i&gt; (&lt;8 nm) multimodal C′ dots with surface-conjugated doxorubicin (DOX) via pH-sensitive linkers for the efficacious treatment in two different clinically relevant high-grade glioma models.&lt;/p&gt;Results:&lt;p&gt;Optimal drug-per-particle ratios of as-developed nanoparticle–drug conjugates were established and used to obtain favorable pharmacokinetic profiles. The &lt;i&gt;in vivo&lt;/i&gt; efficacy results showed significantly improved biological, therapeutic, and toxicological properties over the native drug after intravenous administration in platelet-derived growth factor–driven genetically engineered mouse model, and an EGF-expressing patient-derived xenograft (EGFR PDX) model.&lt;/p&gt;Conclusions:&lt;p&gt;Ultrasmall C′ dot–drug conjugates showed great translational potential over DOX for improving the therapeutic outcome of patients with high-grade gliomas, even without a cancer-targeting moiety.&lt;/p&gt;&lt;/div&gt;
Prostate-specific antigen and prostate cancer (1992)

In 1992, prostate-specific antigen (PSA) is clearly the 'gold standard' tumor marker for prostate cancer and has achieved widespread clinical use. Recent investigations have demonstrated PSA to be useful for diagnosing and staging prostate cancer as well as for monitoring response to treatment. Without question, the discovery of PSA represents a major advancement for the field of prostate cancer and is most likely responsible, in part, for the increasing number of prostate cancers that are diagnosed each year. Prostate cancer is currently the most common malignancy in adult men and the second leading cause of cancer deaths in this population. Since there is no curative therapy for advanced disease, a decrease in the mortality rate can only occur by detecting tumors when they are still organ-confined and then administering effective treatments. PSA and digital rectal examination (DRE) are the mainstay of any early detection program, whereas radical surgery and radiation therapy comprise the therapeutic armamentarium. With the recent development of the 'anatomical' radical prostatectomy, incontinence, impotence, and significant blood loss are no longer frequent complications of this surgery. As a result, radical prostatectomy, via either the retropubic or perineal approach, is being used with increasing frequency as the definitive treatment for clinically localized prostate cancer.
Method to estimate long‐term change of heat and electric power daily load curves in Japan (2005)

Abstract The rapid spread of CHP systems will put pressure on the regional power system to require an examination of the power and heat output of CHP systems. When considering the countrywide potential of the CHP system, one should examine such a system in coordination with the grid power system. It is essential to calculate the heat and power demand at end‐use level. In this paper, annual heat and power demands of end‐use sectors are forecast to the year 2025 based on 20‐year data. Regression analysis is used. Estimated annual demands are divided into the seasonal hourly demands considering demand characteristics. Daily load curves of heat and power demands are determined for the Japanese end‐use sectors, and the annual changes of such demands are shown by duration curves of heat‐to‐power ratios. Moreover, the grid power daily load curves are computed numerically from the estimated heat and power demands for the manufacturing, residential, and commercial sectors. Such load curves also consider self‐generated power for the manufacturing industry and self‐consumption of the grid power. Estimating heat and power demands allows for a joint analysis between the power system and the future phasing in of CHP systems. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 153(3): 18–30, 2005; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20162
Plasma diagnostic x-ray tomography system (1991)

A radiation-hardened 60-channel x-ray tomography system has been developed to determine the two-dimensional distribution of x-ray emissivity from magnetically confined plasmas. In order to maximize their field of view, the diode arrays are mounted in re-entrant tubes inside the plasma chamber diagnostic ports. Metal foil vacuum windows serve as x-ray filters and permit the diodes and cables to be at atmospheric pressure. Preamplifiers are mounted at the outside end of the re-entrant tubes. The diode arrays and preamplifiers are protected from the harsh radiation environment by lead shielding. Image reconstruction is done using the harmonic expansion method or the linear algebraic method. For plasmas with some cylindrical symmetry the harmonic expansion method is superior, but for small discrete objects the Cartesian algebraic method is better. Preliminary data from the Missouri Magnetic Mirror plasma with electron cyclotron resonance heating show evidence of a hot electron ring.
Wall Compensation for Ultra Wideband Applications (2012)

Due to their low frequency contents, ultra wideband (UWB) signals have the ability to penetrate walls and obstacles. As the signal propagates through these obstacles, it gets attenuated, slows down, and gets dispersed. This paper demonstrates wall compensation for through-wall imaging, localization and communication receiver design purposes by first characterizing wave propagation through various building materials in the UWB frequency range. Knowledge of the walls obtained from the wall characterization is used to estimate and correct the position accuracy of a target object located behind the walls using three proposed methods namely; constant amplitude and delay (CDL), frequency dependent data (FFD), and data fitting methods (FIT). The obtained results indicated relatively acceptable measure of wall compensation for the three methods. Results from such work provide insight on how to develop algorithms for effective target position estimation in imaging and localization applications. They are also useful for channel modelling and link budget analysis.
Historical Observations on the Discovery of Platelets, Platelet Function Testing and the First Antiplatelet Agent (2011)

An understanding of the historical paths that have lead to our current state of knowledge in the field of platelet studies can be both illuminating and inspiring. Considering that the existence and function of platelets were initially described just barely over 100 years ago it is exciting to recognize how far our knowledge has advanced in such a relatively short period of time. Within 20 years of Giulio Bizzozero's definitive description of blood platelets investigators began to develop tests that could quantitate the relationship between platelets, hemostasis and bleeding, and these tests have continued to be refined ever since. At the same time, and well before the role of platelets and antiplatelet agents in cardiovascular disease was appreciated, several clinicians started using aspirin for the prevention of heart attacks. All three of these paths of research - platelet biology, platelet function testing and antiplatelet therapies - all converge on what is arguably one of the most important questions in clinical medicine today: how to best prevent arterial thrombosis. For the current and future pioneers of platelet research an understanding of how we got to where we are today will hopefully allow for a clearer and inspired vision of where we will go next.
Synthesis of Partially Hydrogenated Cycloparaphenylenes with Bent and Fused Structures Bearing Armchair Carbon Nanotube-like Connections (2016)

The Diels-Alder reactions between 2 equiv of (E,E)-1,4-bis(4-bromophenyl)-1,3-butadiene and 1,4-benzoquinone led to the formation of a key intermediate with all four 4-bromophenyl substituents cis to one another. The subsequent nickel-mediated homocoupling reactions then produced partially hydrogenated cycloparaphenylenes, including a molecule bearing two units of tetrahydro[6]cycloparaphenylene (4H[6]CPP) fused together through two 1,4-dimethoxybenzene units in an armchair (6,6)carbon nanotube-like connection. Similarly, two 6H[9]CPPs were connected through three 1,4-dimethoxybenzene units in an armchair (9,9)carbon nanotube-like arrangement. A bent 8H[12]CPP and a bent 12H[18]CPP, which were fused intramolecularly with two and three 1,4-dimethoxybenzene units, respectively, to create the bent structures, were likewise synthesized. A molecule containing a bent 8H[12]CPP fused to a 4H[6]CPP was likewise constructed. The structures of these partially hydrogenated CPPs were established by X-ray structure analysis, NMR spectroscopy, and additional independent synthetic pathways.
Desarrollo de alimentos funcionales adaptados a nuevos avances en la genética (2020)

III Congreso de Alimentación, Nutrición y Dietética. Combinar la nutrición comunitaria y personalizada: nuevos retos.
Benzoquinone imines. Part XII. Reactions of 2-aminoindamines [2-amino-N-(4-aminophenyl)-p-benzoquinone di-imines] in aqueous solution (1975)

The fate of 2-aminoindamines [2-amino-N-(4-aminophenyl)-p-benzoquinone di-imines] in aqueous solution has been found to be highly pH dependent. At high pH (>9) the major reaction involves hydrolysis of the unsubstituted imino-group to produce 2-aminoindoanilines [2-amino-N-(4-aminophenyl)-p-benzoquinone mono-imines]. At low pH ( <4), the major reaction involves hydrolysis of the azomethine bridge to give a mixture of a p-phenylenediamine and a 2-amino-p-benzoquinone monoimine. In the intermediate pH range, the amino-indamines undergo intramolecular cyclisation to give 2,8-diaminophenazines. Mechanisms are proposed for these reactions on the basis of kinetic studies of the effect of pH on the rate.