gebhart/patrepeval huggingface dataset:
On-Orbit Prospective Echocardiography on International Space Station Crew (2010)

Introduction A prospective trial of echocardiography was conducted on of six crewmembers onboard the International Space Station. The main objective was to determine the efficacy of remotely guided tele-echocardiography, including just-in-time e-training methods and determine what normal echocardiographic data is. Methods Each crewmember operator (n=6) had 2-hour preflight training. Baseline echocardiographic data were collected 55 to 167days preflight. Similar equipment was used in each 60-minute in-flight session (mean microgravity exposure - 114 days (34 -- 190)). On Orbit ultrasound operators used an e-learning system within 24h of these sessions. Expert assistance was provided using ultrasound video downlink and two-way voice. Testing was repeated 5 to 16 days after landing. Separate ANOVA was used on each echocardiographic variable (n=33). Within each ANOVA, three tests were made: a) effect of mission phase (preflight, in-flight, post flight); b) effect of echo technician (two technicians independently analyzed the data); c) interaction between mission phase and technician. Results Nine rejections of the null hypothesis (mission phase or technician or both had no effect) were discovered and considered for follow up. Of these, six rejections were for significant technician effects, not as a result of space flight. Three rejections of the null hypothesis (Aortic Valve time velocity integral, Mitral E wave Velocity and heart rate) were attributable to space flight, however determined not to be clinically significant. No rejections were due to the interaction between technician and space flight. Conclusion No consistent clinically significant effects of long-duration space flight were seen in echocardiographic variables of the given group of subjects.
Functional photoacoustic microscopy of pH (2012)

pH is a tightly regulated indicator of metabolic activity. In mammalian systems, imbalance of pH regulation may result from or result in serious illness. Even though the regulation system of pH is very robust, tissue pH can be altered in many diseases such as cancer, osteoporosis and diabetes mellitus. Traditional high-resolution optical imaging techniques, such as confocal microscopy, routinely image pH in cells and tissues using pH sensitive fluorescent dyes, which change their fluorescence properties with the surrounding pH. Since strong optical scattering in biological tissue blurs images at greater depths, high-resolution pH imaging is limited to penetration depths of 1mm. Here, we report photoacoustic microscopy (PAM) of commercially available pH-sensitive fluorescent dye in tissue phantoms. Using both opticalresolution photoacoustic microscopy (OR-PAM), and acoustic resolution photoacoustic microscopy (AR-PAM), we explored the possibility of recovering the pH values in tissue phantoms. In this paper, we demonstrate that PAM was capable of recovering pH values up to a depth of 2 mm, greater than possible with other forms of optical microscopy.
Diversity of TITAN Functions in Arabidopsis Seed Development (2002)

The titan mutants of Arabidopsis exhibit striking defects in seed development. The defining feature is the presence of abnormal endosperm with giant polyploid nuclei. Several TTN genes encode structural maintenance of chromosome proteins (condensins and cohesins) required for chromosome function at mitosis. Another TTN gene product (TTN5) is related to the ARL2 class of GTP-binding proteins. Here, we identify four additional TTN genes and present a general model for the titan phenotype. TTN1 was cloned after two tagged alleles were identified through a large-scale screen of T-DNA insertion lines. The predicted gene product is related to tubulin-folding cofactor D, which interacts with ARL2 in fission yeast (Schizosaccharomyces pombe) and humans to regulate tubulin dynamics. We propose that TTN5 and TTN1 function in a similar manner to regulate microtubule function in seed development. The titan phenotype can therefore result from disruption of chromosome dynamics (ttn3, ttn7, and ttn8) or microtubule function (ttn1 and ttn5). Three other genes have been identified that affect endosperm nuclear morphology. TTN4 and TTN9 appear to encode plant-specific proteins of unknown function. TTN6 is related to the isopeptidase T class of deubiquitinating enzymes that recycle polyubiquitin chains following protein degradation. Disruption of this gene may reduce the stability of the structural maintenance of chromosome complex. Further analysis of the TITAN network should help to elucidate the regulation of microtubule function and chromosome dynamics in seed development.
Oxygen chiral phosphodiesters. 2. Enzymic synthesis and configurational analysis of [.alpha.-18O]-2'-deoxyadenosine 5'-diphosphate (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOxygen chiral phosphodiesters. 2. Enzymic synthesis and configurational analysis of [.alpha.-18O]-2'-deoxyadenosine 5'-diphosphateJeffrey A. Coderre and John A. GerltCite this: J. Am. Chem. Soc. 1980, 102, 21, 6594–6597Publication Date (Print):October 8, 1980Publication History Published online1 May 2002Published inissue 8 October 1980https://pubs.acs.org/doi/10.1021/ja00541a053https://doi.org/10.1021/ja00541a053research-articleACS PublicationsRequest reuse permissionsArticle Views61Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Rostro-Caudal Specificity of Corticospinal Tract Projections in Mice (2020)

Rostro-caudal specificity of corticospinal tract (CST) projections from different areas of the cortex was assessed by retrograde labeling with fluorogold and retrograde transfection following retro-AAV/Cre injection into the spinal cord of tdT reporter mice. Injections at C5 led to retrograde labeling of neurons throughout forelimb area of the sensorimotor cortex and a region in the dorsolateral cortex near the barrel field (S2). Injections at L2 led to retrograde labeling of neurons in the posterior sensorimotor cortex (hindlimb area) but not the dorsolateral cortex. With injections of biotinylated dextran amine (BDA) into the main sensorimotor cortex (forelimb region), labeled axons terminated selectively at cervical levels. With BDA injections into caudal sensorimotor cortex (hindlimb region), labeled axons passed through cervical levels without sending collaterals into the gray matter and then elaborated terminal arbors at thoracic sacral levels. With BDA injections into the dorsolateral cortex near the barrel field, labeled axons terminated at high cervical levels. Axons from medial sensorimotor cortex terminated primarily in intermediate laminae and axons from lateral sensorimotor cortex terminated primarily in laminae III-V of the dorsal horn. One of the descending pathways seen in rats (the ventral CST) was not observed in most mice.
Effect of medical staff training on vaccination coverage in outpatients with cancer: An interventional multicenter before-and-after study (2023)

As expected, VC was very low in patients with cancer, consistent with the literature. There was no impact of the intervention for pneumococcal and influenza VC.
Profile-based dynamic voltage and frequency scaling for a multiple clock domain microprocessor (2003)

A Multiple Clock Domain (MCD) processor addresses the challenges of clock distribution and power dissipation by dividing a chip into several (coarse-grained) clock domains, allowing frequency and voltage to be reduced in domains that are not currently on the application’s critical path. Given a reconfiguration mechanism capable of choosing appropriate times and values for voltage/frequency scaling, an MCD processor has the potential to achieve significant energy savings with low performance degradation. &lt;BR&gt;&lt;BR&gt; Early work on MCD processors evaluated the potential for energy savings by manually inserting reconfiguration instructions into applications, or by employing an oracle driven by off-line analysis of (identical) prior program runs. Subsequent work developed a hardware-based on-line mechanism that averages 75–85% of the energy-delay improvement achieved via off-line analysis. &lt;BR&gt;&lt;BR&gt; In this paper we consider the automatic insertion of reconfiguration instructions into applications, using profiledriven binary rewriting. Profile-based reconfiguration introduces the need for “training runs” prior to production use of a given application, but avoids the hardware complexity of on-line reconfiguration. It also has the potential to yield significantly greater energy savings. Experimental results (training on small data sets and then running on larger, alternative data sets) indicate that the profile-driven approach is more stable than hardware-based reconfiguration, and yields virtually all of the energy-delay improvement achieved via off-line analysis.
Distributed cyber attack detection for power network systems (2011)

In this paper, a framework of distributed fault detection for network systems is developed. Specifically, a framework of distributed cyber attack detection system for synchronized large-scale power network is constructed, where active power flow in power network system is modeled by the swing equation and cyber attacks are modeled as unknown power generation or consumption. The approach is based on fault detection and identification (FDI) filter so that malicious cyber attacks in the neighborhood of a node are identified through local information that consists of local power consumption, generation, and power flow. The FDI filter is a special Luenberger observer whose parameter is selected in such a way that residual between the sensed power flow and the FDI filter's output is only affected by specific cyber attacks. Residual indicates the existence of the cyber attack. A sufficient condition is provided for the existence of the FDI filter with the local input and the output to detect fault in the network system. A numerical example is provided to demonstrate efficacy of the proposed approach.
Effect of locally-applied active site-blocked activated factor VII (ASIS) on experimental arterial thrombosis (2000)

The starting point of blood coagulation in vivo is the formation of a complex between tissue factor (TF), which is exposed following vascular disease or trauma, and activated blood coagulation factor VII (FVIIa). This blinded, random, paired study evaluates whether active site-blocked activated FVII (FVIIai, ASIS), which binds avidly to TF but is unable to initiate the coagulation processes, inhibits experimental thrombosis. Arteriotomy and deep vessel wall trauma were performed in the central arteries of rabbits' ears. The topical administration of ASIS (0.5 mg in 200 microl vehicle) resulted in a distinct antithrombotic effect compared to vehicle alone. Patency rates at 30 and 120 min after reperfusion were 85% and 75% in the ASIS group and 45% and 30% in the vehicle group, respectively (P = 0.008 and P = 0.004). In contrast, intravenous administration of ASIS (4 mg/kg) produced no antithrombotic effect. Arteriotomy bleeding times were 1.5 min in the ASIS group and 2.0 min in the vehicle group (medians, P = 1). Local application of ASIS produces a pronounced antithrombotic effect in rabbits without giving rise to antihaemostatic side-effects. This mode of treatment may have a potential for a variety of clinical interventions in injured or diseased vessels.
Two-Step Purification and Partial Characterization of Keratinolytic Proteases from Feather Meal Bioconversion by Bacillus sp. P45 (2023)

This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude protease extract was purified using a sequence of an aqueous two-phase system (ATPS) in large volume systems (10, 50, and 500 g) to increase obtaining purified enzyme, followed by a diafiltration (DF) step. Purified protease was characterized in terms of protein profile analysis by SDS-PAGE, optimum temperature and pH, thermal deactivation kinetics at different temperatures and pH, and performance in the presence of several salts (NaCl, CaCl2, MnCl2, CaO, C8H5KO4, MgSO4, CuSO4, ZnSO4, and FeCl3) and organic solvents (acetone, ethanol, methanol, acetic acid, diethyl ether, and formaldehyde). ATPS with high capacities resulted in purer protease extract without compromising purity and yields, reaching a purification factor up to 2.6-fold and 6.7-fold in first and second ATPS, respectively, and 4.0-fold in the DF process. Recoveries were up to 79% in both ATPS and reached 84.3% after the DF step. The electrophoretic analysis demonstrated a 25–28 kDa band related to keratinolytic protease. The purified protease’s optimum temperature and pH were 55 °C and 7.5, respectively. The deactivation energy (Ed) value was 118.0 kJ/mol, while D (decimal reduction time) and z (temperature interval required to reduce the D value in one log cycle) values ranged from 6.7 to 237.3 min and from 13.6 to 18.8 °C, respectively. Salts such as CaCl2, CaO, C8H5KO4, and MgSO4 increased the protease activity, while all organic solvents caused its decrease. The results are useful for future studies about ATPS scale-up for enzyme purification and protease application in different industrial processes.
On the calculation of the meta-converse. (2015)

Saddle point in the second form of the minimax converse is proven. We describe and algorithm for the computation of the saddle point distributions.
Application of the concise exposure index (OCRA) to tasks involving repetitive movements of the upper limbs in a variety of manufacturing industries: preliminary validations (1998)

A summary of eight investigations is presented, which were carried out using standardized methods, for the purpose of quantifying exposure to tasks involving repetitive movements of the upper limbs, as well as quantifying the prevalence of work-related musculoskeletal disorders (WMSDs) of the upper limbs in groups of exposed workers. A total of 462 exposed workers were examined, and the study also took into account the data pertaining to a matched reference group comprising 749 workers not exposed to any specific occupational risk. Regarding the quantification of exposure to increased risk, use was made of a concise index (OCRA index), proposed by Occhipinti, in this issue. The data resulting from the eight investigations were used for the study of measurements and models of association among the exposure variables (mainly represented by the OCRA index), as well as the effect variables represented by the prevalence of the various WMSDs of the upper limbs taken both individually and jointly. Significant associations were reported between the OCRA index and an effect indicator represented by the prevalence of all the WSMDs of the upper limbs, calculated on the number of upper limbs at risk. When a logarithmic conversion of the relative exposure (OCRA) and injury indices was carried out, a simple linear regression model resulted that seems to provide a satisfactory predictive performance of the risk of WMSDs of the upper limbs, based on the exposure index. The study confirmed the efficacy of various other models designed to predict effects based on multiple linear regression functions, in which the independent variables are represented by both the OCRA exposure index and by parameters relative to the breakdown by gender and age of the groups of exposed workers.
Suprabasal Dsg2 expression in transgenic mouse skin confers a hyperproliferative and apoptosis-resistant phenotype to keratinocytes (2007)

Desmoglein 2 (Dsg2), a component of the desmosomal cell-cell adhesion structure, has been linked to invasion and metastasis in squamous cell carcinomas. However, it is unknown whether--and if so how--Dsg2 contributes to the malignant phenotype of keratinocytes. In this study, we addressed the consequences of Dsg2 overexpression under control of the involucrin promoter (Inv-Dsg2) in the epidermis of transgenic mice. These mice exhibited epidermal hyperkeratosis with slightly disrupted early and late differentiation markers, but intact epidermal barrier function. However, Inv-Dsg2 transgene expression was associated with extensive epidermal hyperplasia and increased keratinocyte proliferation in basal and suprabasal epidermal strata. Cultured Inv-Dsg2 keratinocytes showed enhanced cell survival in the anchorage-independent state that was critically dependent on EGF receptor activation and NF-kappaB activity. Consistent with the hyperproliferative and apoptosis-resistant phenotype of Inv-Dsg2 transgenic keratinocytes, we observed enhanced activation of multiple growth and survival pathways, including PI 3-kinase/AKT, MEK-MAPK, STAT3 and NF-kappaB, in the transgenic skin in situ. Finally, Inv-Dsg2 transgenic mice developed intraepidermal skin lesions resembling precancerous papillomas and were more susceptible to chemically induced carcinogenesis. In summary, overexpression of Dsg2 in epidermal keratinocytes deregulates multiple signaling pathways associated with increased growth rate, anchorage-independent cell survival, and the development of skin tumors in vivo.
Superconducting microstrip detectors : Addendum to proposal DRDC-P-53 (1998)

The recent advances in Si and diamond detector technology give hope of a simple solution to the radiation hardness problem for vertex trackers at the LHC. In particular, we have recently demonstrated that operating a heavily irradiated Si detector at liquid nitrogen (LN2) temperature results in significant recovery of Charge Collection Efficiency (CCE). Among other potential benefits of operation at cryogenic temperatures are the use of large low-resistivity wafers, simple processing, higher and faster electrical signal because of higher mobility and drift velocity of carriers, and lower noise of the readout circuit. A substantial reduction in sensor cost could result. Several CERN experiments are potential users of cold radiation hard tracking devices. The first goal of the proposed extension of the RD39 programme is to demonstrate that irradiation at low temperature in situ during operation does not affect the results obtained so far by cooling detectors which were irradiated at room temperature. In particular we shall concentrate on processes and materials that could significantly reduce the final detector cost. The second goal is to demonstrate the operation of existing radiation-hard CMOS readout electronics at LN2 temperature, and to measure discrete device characteristics at these temperatures, so that their parameters can be extracted and optimised circuits can be designed. The third goal is to demonstrate that low-mass cooling at LN2 temperature is feasible at a reasonable cost, and that the electrical and optical feedthroughs of a large system can be mastered.
Interferon-β Therapy Against EAE Is Effective Only When Development of the Disease Depends on the NLRP3 Inflammasome (2012)

Interferon-β (IFN-β) is widely used to treat multiple sclerosis (MS), and its efficacy was demonstrated in the setting of experimental autoimmune encephalomyelitis (EAE), an animal model of MS; however, IFN-β is not effective in treating all cases of MS. Here, we demonstrate that signaling by IFNAR (the shared receptor for IFN-α and IFN-β) on macrophages inhibits activation of Rac1 and the generation of reactive oxygen species (ROS) through suppressor of cytokine signaling 1 (SOCS1). The inhibition of Rac1 activation and ROS generation suppressed the activity of the Nod-like receptor (NLR) family, pyrin domain-containing 3 (NLRP3) inflammasome, which resulted in attenuated EAE pathogenicity. We further found that two subsets of EAE could be defined on the basis of their dependency on the NLRP3 inflammasome and that IFN-β was not an effective therapy when EAE was induced in an NLRP3 inflammasome-independent fashion. Thus, our study demonstrates a previously uncharacterized signaling pathway that is involved in the suppression of EAE by IFN-β and characterizes NLRP3-independent EAE, which cannot be treated with IFN-β.
Modification of commercially available low-loss SAW devices towards an immunosensor for in-situ measurements in water (2002)

An acoustoelectric immunosensor which closely resembles the "classic" surface acoustic wave (SAW) technique for gas detection is presented. The device operates at frequencies above 100 MHz and allows in-situ measurements in water with a typical additional attenuation of only 4 dB compared to that in air. This is achieved by using commercially available low-loss SAW devices operating with horizontally polarized surface transverse waves (STW) on a lithium tantalate (LiTaO/sub 3/) substrate. The devices were used as the frequency determining element of an oscillator circuit. Modifications of the devices towards an immunosensor are done in three steps: Precoating with a shielding layer of polyimide to prevent corrosion of the aluminium transducers, chemical activation of the polyimidized surface and subsequent covalent precipitation of an antibody layer. The immuno-specific adsorption of antigens from solution can be monitored directly by the decrease in resonant frequency arising from the surface mass loading.
On Combining Data for Estimating the Frequency of Low-Probability Events with Application to Sodium Valve Failure Rates (1983)

Probabilistic risk assessments are increasingly being used to quantify the public risks of operating potentially hazardous systems such as nuclear power reactors. Such assessments require the quantification of the frequencies of various low-probability events.In performing these analyses, the risk analyst is often confronted with the dual problem of the appropriate data to be used to estimate the required frequencies and the development of the corresponding estimates. Often the problem reduces to one of how to combine (or pool) a variety of more or less applicable existing data sources.A Bayes/empirical-Bayes procedure is developed for combining as many as five different types of pertinent data. The five data types can be grouped under 1. analysis data,2. similar operating data,3. expert opinions,4. historical operating data,5. generic data.Example illustrations of each of these data types are given.The procedure is used to estimate the combined hourly failure rate of small manually operated sodium valves, such as those typically found in liquid-metal fast breeder reactor shutdown heat removal systems. Pertinent data sources include operating data from sodium test loops (similar operating data), expert opinion, operating data from the Experimental Breeder Reactor-II, and seven generic failure rate estimates for similar valves in both U.K. and U.S. operating light water power reactors. A final posterior distribution is produced that reflects the combined influence of all of these data. This distribution provides the required estimates and corresponding uncertainty bounds.