gebhart/patrepeval huggingface dataset:
Kinetic and physical properties of a recombinant RuBisCO from a chemoautotrophic endosymbiont. (1993)

Ribulose 1,5 bisphosphate carboxylase/oxygenase (RuBisCO), which catalyzes the key step in autotrophic CO2 fixation, is present at high activity in the symbiont-containing tissues of many hydrothermal vent invertebrates. The genes encoding RuBisCO from a gill endosymbiont of the hydrothermal vent gastropod Alviniconcha hessleri have been cloned, sequenced, and functionally expressed in Escherichia coli under control of the lac promoter in the vector pBlueScript. The purified protein is a hexadecamer (L8S8) with an apparent molecular weight of 554 kDa and a specific carboxylase activity of 2.9 mumol/min/mg protein. Unlike previously characterized RuBisCOs, which display sharp temperature optima, the symbiont RuBisCO maintains a high level of activity over a broad temperature span, although it is not thermally stable after extended exposure to temperatures above 50 degrees C. This funding suggests an adaptation to the thermal transients measured at vent openings where the host snail resides. The enzyme also maintains 90% of its 1 atm activity at 370 atm pressure, whereas spinach RuBisCO retains 28% activity under similar conditions. At 1 atm pressure and 20 degrees C, the Km(CO2) and the relative substrate specificity (Srel) of the symbiont enzyme were 80 and 32.5 mumol/L, respectively, which are similar to values reported for RuBisCOs from cyanobacteria and the purple photosynthetic bacteria. The relatively low specificity of the enzyme for substrate CO2 indicates that the intracellular environment of the endosymbionts may be microaerophilic for RuBisCO to maintain net carboxylation.
Alteration of the Cell Surface during the Vegetative Cell Cycle of the Unicellular Green Alga Chlamydomonas reinhardtii (1996)

Alterations of the cell surface during the vegetative cell cycle of the unicellular green alga Chlamydomonas reinhardtii were investigated using polyclonal antibodies against the purified and subsequently deglycosylated insoluble cell wall component and against a 100 kDa polypeptide of the deglycosylated, chaotrope-soluble wall fraction, respectively. Both antibodies recognized epitopes within the non-glycosylated domains of a ‘150 kDa’ chaotrope-soluble glycoprotein (=GP3B) localized in the outer layers of the C. reinhardtii cell wall. Immunofluorescence studies indicated that both antibodies reacted with the surface of ‘late’ sporangia (harvested 1 h before liberation of the zoospores), but not with the cell surfaces of released zoospores, growing cells and young sporangia, respectively. After pretreatment with aqueous LiCl, however, the cell surfaces of zoospores, growing cells and young sporangia became accessible to these particular antibodies. Highly purified preparations of the insoluble wall fraction revealed strong immunofluorescence with both antibodies but not with the corresponding preimmune sera. Based on these data, we concluded that the antigenic sites of the insoluble glycoprotein framework of the C. reinhardtii wall are masked by LiCl-soluble glycoproteins in single cell stages and young sporangia, but not or to a lesser extent in the case of the mother walls of ‘late’ sporangia. The conclusion was supported by findings that (I) the multilayered structure of the mother-cell wall was disturbed in ‘late’, but not in young sporangia and that (II) the amounts of chaotropesoluble cell wall glycoproteins present in the LiCl-extracts from intact sporangia decreased during ripening of the sporangia.
Présentation (2015)

L’histoire réunit, la mémoire tend à diviser. Cette affirmation, presque un aphorisme, forgé par Paul Ricœur dans son ouvrage La Mémoire, l’histoire, l’oubli, est devenu un leitmotiv des approches historiennes du temps présent. Il n’en renvoie pas moins à une question dont l’acuité reste grande tant il est à mettre en relation avec les divers usages de passés systématiquement recomposés à l’aune de politiques ou de stratégies mémorielles dont les inspirateurs ou les initiateurs poursuivent de...
Barrier lowering and reorientation of dipoles grafted at indium–tin–oxide/polymer interfaces (2002)

Dipolar molecules have been grafted on the surface of indium–tin–oxide plates. Using these modified substrates as anodes for organic diodes made from poly–vinylcarbazole enhances the hole current by 1–2 orders of magnitude as compared to diodes with nonmodified anodes. The organization and surface concentration of the grafted monolayer, determined by infrared spectroscopy, show that the grafted layer is not densely packed. The influence of the dipolar molecules on the energy barrier height for the injection of hole current into the organic layer is also measured by internal photoemission, evidencing that the barrier is lowered by the molecular grafting. The free space around the grafted molecules allows for their reorientation under electric field. The energy barrier height has been measured as a function of time during this reorientation.
Intravenous infusion of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells as a potential treatment for patients with COVID-19 pneumonia (2020)

The novel coronavirus disease 2019 (COVID-19) has grown to be a global public health emergency since patients were first detected in Wuhan, China. Thus far, no specific drugs or vaccines are available to cure the patients with COVID-19 infection. The immune system and inflammation are proposed to play a central role in COVID-19 pathogenesis. Mesenchymal stem cells (MSCs) have been shown to possess a comprehensive powerful immunomodulatory function. Intravenous infusion of MSCs has shown promising results in COVID-19 treatment. Here, we report a case of a severe COVID-19 patient treated with human umbilical cord Wharton's jelly-derived MSCs (hWJCs) from a healthy donor in Liaocheng People's Hospital, China, from February 24, 2020. The pulmonary function and symptoms of the patient with COVID-19 pneumonia was significantly improved in 2 days after hWJC transplantation, and recovered and discharged in 7 days after treatment. After treatment, the percentage and counts of lymphocyte subsets (CD3<sup>+</sup>, CD4<sup>+</sup>, and CD8<sup>+</sup> T cell) were increased, and the level of IL-6, TNF-α, and C-reactive protein is significantly decreased after hWJC treatment. Thus, the intravenous transplantation of hWJCs was safe and effective for the treatment of patients with COVID-19 pneumonia, especially for the patients in a critically severe condition. This report highlights the potential of hWJC infusions as an effective treatment for COVID-19 pneumonia.
Parallel Combinatory Multiple-Subcarrier Optical Communication Systems (2001)

We propose a parallel combinatory multiple-subcarrier (PC-MS) optical communication system. The proposed system with N subcarriers selects at each symbol interval a subset of U null subcarriers and transmits /spl lfloor/log/sub 2/ (N/U)/spl rfloor/ bits for the selected subset. The other N-U=N/sub pc/ subcarriers are modulated by points from an M-PSK signal constellation. Thus, the proposed system can transmit more information at each symbol interval than the conventional MS system with N subcarriers. in addition the proposed system can decrease the bias that must be added to the MS electrical signal, because the number of subcarriers other than null subcarriers is smaller than that in the conventional MS systems and thus the minimum power is larger Therefore, the proposed system can decrease the required energy per bit.
Properties of single RuO<inf>2</inf> layer embedded in SrTiO<inf>3</inf> (2015)

A two-dimensional ferromagnet is an interesting system both for basic understanding of magnetism and for spintronic applications. However, making such a system is not easy as ferromagnetism becomes more and more unstable with reducing the thickness of a ferromagnet. For example, in oxide systems such as SrRuO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> , La <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1-x</sub> Sr <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> MnO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> , etc, ferromagnetism is lost around the thickness of a few unit cells [1, 2]. Recent theoretical studies have shown that a single SrRuO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> layer can exhibit half-metallicity when it is sandwiched between SrTiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> lattices [3, 4]. The conduction electrons are predicted to exist only in RuO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> layers, which means that the system is a two-dimensional half-metal. In this work, in order to demonstrate the theoretical prediction, we have fabricated high-quality SrRuO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> /SrTiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> superlattices using molecular beam epitaxy. Magnetic and transport measurements show a clear signature of ferromagnetism and conductivity.
Tandem repeat sequence variation as causative Cis-eQTLs for protein-coding gene expression variation: The case of CSTB (2012)

Association studies have revealed expression quantitative trait loci (eQTLs) for a large number of genes. However, the causative variants that regulate gene expression levels are generally unknown. We hypothesized that copy-number variation of sequence repeats contribute to the expression variation of some genes. Our laboratory has previously identified that the rare expansion of a repeat c.-174CGGGGCGGGGCG in the promoter region of the CSTB gene causes a silencing of the gene, resulting in progressive myoclonus epilepsy. Here, we genotyped the repeat length and quantified CSTB expression by quantitative real-time polymerase chain reaction in 173 lymphoblastoid cell lines (LCLs) and fibroblast samples from the GenCord collection. The majority of alleles contain either two or three copies of this repeat. Independent analysis revealed that the c.-174CGGGGCGGGGCG repeat length is strongly associated with CSTB expression (P = 3.14 × 10(-11)) in LCLs only. Examination of both genotyped and imputed single-nucleotide polymorphisms (SNPs) within 2 Mb of CSTB revealed that the dodecamer repeat represents the strongest cis-eQTL for CSTB in LCLs. We conclude that the common two or three copy variation is likely the causative cis-eQTL for CSTB expression variation. More broadly, we propose that polymorphic tandem repeats may represent the causative variation of a fraction of cis-eQTLs in the genome.
Quantitative Analysis of MR Imaging to Assess Treatment Response for Patients with Multiple Myeloma by Using Dynamic Intensity Entropy Transformation: A Preliminary Study (2015)

This study demonstrated the feasibility of quantitative response index to differentiate responder and nonresponder patients and had substantial agreement with clinical outcomes, which indicated that this quantitative measure has the potential to be an image biomarker to assess MM treatment response.
Stress‐activated expression of a Streptomyces pristinaespiralis multidrug resistance gene (ptr) in various Streptomyces spp. and Escherichia coli (1995)

A promoter which controls expression of the pristinamycin multidrug resistance gene (ptr) in Streptomyces pristinaspiralis could be induced by physiological stresses in both Streptomyces spp. and Escherichia coli. In S. pristinaspiralis, the ptr promoter (Pptr) was induced by pristinamycin I (PI) or pristinamycin II (PII). Streptomyces lividans was adopted as a convenient heterologous host for studies of Pptr regulation since it has no known pristinamycin biosynthetic genes. Two key regulatory features were documented in these studies: many (19 of 70) antibiotics and chemicals with no common targets or structural features induced the Pptr; induction with PI was most efficient during a transition phase when antibiotic biosynthetic genes are switched on. In Streptomyces coelicolor, Pptr activity was similarly inducible by PI and not dependent on sigma factors HrdA, HrdC, or HrdD. In E. coli, Pptr cloned in the bifunctional promoter probe vector pIJ2839 was functional and activated upon entry into stationary phase in the absence of exogenous inducer. Finally, gel-retardation studies demonstrated a Pptr-binding protein in S. lividans (where its activity was PI-inducible), S. coelicolor and S. pristinaespiralis. The fact that this activity was not detected in E. coli suggested the existence of another regulatory system perhaps also present in Streptomyces.
Ultralow-Density, High-Modulus, and High-Strength RSP Al-Li-Be Alloys (1986)

Aluminum-lithium-beryllium alloys have been prepared by melt spinning, using prealloyed arc-melted buttons. The alloy ribbon was comminuted and cold compacted to 30 to 50% density, vacuum hot pressed to 100% density, and extruded. The bar product was subsequently solution heat treated, quenched, and aged. The microstructure of the alloys consists of a relatively featureless matrix containing a homogeneous dispersion of fine beryllium particles, mostly 50 to 500 nm in size. The results of heat treatment show that the alloys respond to age hardening in a manner similar to that of binary Al-Li alloys. Preliminary results on the mechanical properties and their relationships with microstructural features are presented for the Al-Li-Be alloys, as well as their potential for weight savings. The authors estimate that these monolithic aluminum alloys may exhibit specific strength and elastic modulus values comparable to those of the best whisker or particulate metal-matrix composites, while showing concomitant high weight savings.
Strategy for Surface Post-Processing of AISI 316L Additively Manufactured by Powder Bed Fusion Using Ultrasonic Nanocrystal Surface Modification (2021)

Ultrasonic nanocrystal surface modification (UNSM) technology was applied to the surfaces of specimens additively manufactured by powder bed fusion (PBF). The changes in roughness and hardness due to the UNSM were set as objective functions, and the optimal conditions for the main parameters were derived through the response surface method (RSM) and Box–Behnken design (BBD). Regression analysis-based mathematical models for predicting the surface hardness and roughness are presented and validated. The RSM results show that the surface roughness is highly dependent on the load and ball tip diameter, and the surface roughness significantly improves as the inter-pass interval and ball tip diameter decrease. Through BBD and ANOVA, the optimal conditions for the improvement of surface characteristics were found to be a load of 40 N, inter-pass interval of 10 μm, and ball tip diameter of 2.38 m. The surface treated under these optimal conditions exhibited a hardness of 497 Hv and surface roughness of 1.32 μm, which were significantly improved compared to the values for an untreated specimen. In addition, it was confirmed that the grains are significantly refined after UNSM, and scratch resistance increases for the top layer of the surface directly affected by the UNSM horn.
Subregional Specification of Embryonic Stem Cell-Derived Ventral Telencephalic Tissues by Timed and Combinatory Treatment with Extrinsic Signals (2011)

During early telencephalic development, the major portion of the ventral telencephalic (subpallial) region becomes subdivided into three regions, the lateral (LGE), medial (MGE), and caudal (CGE) ganglionic eminences. In this study, we systematically recapitulated subpallial patterning in mouse embryonic stem cell (ESC) cultures and investigated temporal and combinatory actions of patterning signals. In serum-free floating culture, the dorsal-ventral specification of ESC-derived telencephalic neuroectoderm is dose-dependently directed by Sonic hedgehog (Shh) signaling. Early Shh treatment, even before the expression onset of Foxg1 (also Bf1; earliest marker of the telencephalic lineage), is critical for efficiently generating LGE progenitors, and continuous Shh signaling until day 9 is necessary to commit these cells to the LGE lineage. When induced under these conditions and purified by fluorescence-activated cell sorter, telencephalic cells efficiently differentiated into Nolz1(+)/Ctip2(+) LGE neuronal precursors and subsequently, both in culture and after in vivo grafting, into DARPP32(+) medium-sized spiny neurons. Purified telencephalic progenitors treated with high doses of the Hedgehog (Hh) agonist SAG (Smoothened agonist) differentiated into MGE- and CGE-like tissues. Interestingly, in addition to strong Hh signaling, the efficient specification of MGE cells requires Fgf8 signaling but is inhibited by treatment with Fgf15/19. In contrast, CGE differentiation is promoted by Fgf15/19 but suppressed by Fgf8, suggesting that specific Fgf signals play different, critical roles in the positional specification of ESC-derived ventral subpallial tissues. We discuss a model of the antagonistic Fgf8 and Fgf15/19 signaling in rostral-caudal subpallial patterning and compare it with the roles of these molecules in cortical patterning.
Time-Resolved Far Infrared Light Transport Decomposition for Thermal Photometric Stereo (2019)

We present a novel time-resolved light transport decomposition method using thermal imaging. Because the speed of heat propagation is much slower than the speed of light propagation, the transient transport of far infrared light can be observed at a video frame rate. A key observation is that the thermal image looks similar to the visible light image in an appropriately controlled environment. This implies that conventional computer vision techniques can be straightforwardly applied to the thermal image. We show that the diffuse component in the thermal image can be separated, and therefore, the surface normals of objects can be estimated by the Lambertian photometric stereo. The effectiveness of our method is evaluated by conducting real-world experiments, and its applicability to black body, transparent, and translucent objects is shown.
Bureau of mines coal cutting technology facilities at the Twin Cities Research Center. Information circular/1983 (1983)

Research on coal cutting at the Bureau of Mines Twin Cities Research Center (TCRC) has evolved from a purely mechanical approach, specifically to reduce dust or frictional methane ignitions, into an understanding of the complexity of the cutting system relationships. Achieving an understanding of these relationships requires a wide variety of testing techniques and equipment. Laboratory facilities and the associated equipment exist for shallow to deep cutting in both coal and coal-inclusive rock with any desired bit type. Research efforts with this equipment are providing insight for significant advances to help solve the problems of pneumoconiosis and frictional ignition. This effort will ultimately affect both respirable dust and methane ignitions at the face through better bit design and will increase the salable percent of run-of-mine (ROM) coal processed. It will also affect the design of rotary-drum cutting continuous mining machines (CMM) and longwall machines. This report describes the main features of the coal cutting research facilities at TCRC.
A class of DC-free subcodes of convolutional codes (1996)

We describe a class of DC-free subcodes of convolutional codes that satisfy certain runlength constraints and that also possess error-correcting capability. The running disparity and the maximum runlength of these codes are bounded by quantities that are independent of the free distance. They can be decoded using a Viterbi decoder for the underlying convolutional code.
DEPRESIVNI POREMEĆAJI КAО REPERKUSIJA MOBINGA (2010)

Novija istraživanja i naučne studije pokazuju da su radno aktivne osoba u značajnom broju žrtve mobinga sa posljedicama na psihičko zdravlje i sa polimorfnom klimfikom simptomatologijom i smetnjama na porodičnosocijalnoj ravni funkcionisanja.CILJ: Osnovni eiij ovog istraživanja, koje je po svojoj strukluri klmička deskriptivno, bio jc uivrditi posljedice mobinga na psihičko stanje.MATERIJAL I METODE: Ukupan uzorak je 87 ispitanika. dobi od i K-65 godina. oba pola. radno akiivni. različitog socijalnog staiusa i kuimrološkili navika. Liječeni su radi različitih psiliijatrijskili sindroma sa zajednickim nazivnikom - mobingom. kao etiopatogeneiskim fakiorom, Od insinmienata istraživanja koriSteni su: Upitnik sociodemografskih podataka. Klasični psihijatrijski intervju. Beckova skala za samoproejenu depresije i Hamilton ova skala za depresiju (HAM-D).U radu su prikazana iskuslva. primjena i učinci razliCilih modela tretmana uz uključlvanje psihometrijskih instrumenata, testova t skala. Klijenti-ispitanici su dali svoj pristanak za učešće u istraživanju i iretmanu fenomena mobinga. REZULTATI: Ustanovljeno je da najveći broj ispitanika s mobingom ispoljava depresivne simptome, poiom anksiozne, a iza njih slijede ostali poremećajt.ZAKLJUCAK: Analiza rezultata istraživanja prcma socio-demografskim karakteristikama, vremenskom intervatu izložcnosti mobingu, koriStenim mjernim instrumentima, dijagnostičkim kategorijama i primjenjenim terapijskim mjerama potvrduje potrebu daljeg istraživanja i tretmana žrtava mobinga.
Mechanical monolithic tiltmeter for low frequency measurements (2013)

The paper describes a tilt meter sensor for geophysical applications, based on Folded Pendulum (FP) mechanical sensor. Both the theoretical model and the experimental results of a tunable mechanical monolithic FP tilt meter prototype are presented and discussed. Some of the most important characteristics, like the measured resolution of &asymp; 0.1 <i>nrad</i> at 100<i>mHz</i>, are detailed. Among the scientific results, earth tilt tides have been already observed with this monolithic FP tilt meter prototype.
Structure-based mechanism of lipoteichoic acid synthesis by Staphylococcus aureus LtaS (2009)

Staphylococcus aureus synthesizes polyglycerol-phosphate lipoteichoic acid (LTA) from phosphatidylglycerol. LtaS, a predicted membrane protein with 5 N-terminal transmembrane helices followed by a large extracellular part (eLtaS), is required for staphylococcal growth and LTA synthesis. Here, we report the first crystal structure of the eLtaS domain at 1.2-A resolution and show that it assumes a sulfatase-like fold with an alpha/beta core and a C-terminal part composed of 4 anti-parallel beta-strands and a long alpha-helix. Overlaying eLtaS with sulfatase structures identified active site residues, which were confirmed by alanine substitution mutagenesis and in vivo enzyme function assays. The cocrystal structure with glycerol-phosphate and the coordination of a Mn(2+) cation allowed us to propose a reaction mechanism, whereby the active site threonine of LtaS functions as nucleophile for phosphatidylglycerol hydrolysis and formation of a covalent threonine-glycerolphosphate intermediate. These results will aid in the development of LtaS-specific inhibitors for S. aureus and many other Gram-positive pathogens.