gebhart/patrepeval huggingface dataset:
A 6 &#215; 6-in 20-lpi electroluminescent display panel (1975)

Over the last two years, we have been working on a solution of the solid-state display addressing problem which consists of building the addressing circuits (and eventually, also scanning or decoding circuits) directly on the panel, and fully integrated with the particular display medium. The technique utilized for this approach is that of a vacuum-deposited thin-film transistor matrix. In this paper, we report on the design, construction, and performance of a 12 000 element EL panel, suitable for alphanumeric, vectorgraphic, and monochrome TV image presentation. The basic circuit, repeated at every picture element, consists of an X-Y-addressed logic transistor, a power transistor, and a storage capacitor. The entire circuit was fabricated through multiple evaporations in a multisource system using one pumpdown cycle. The finished thin-film circuit is covered with a sprayed EL-phosphor. An evaporated Au/PbO layer forms the continuous top electrode. The entire display panel is finally sealed with a glass cover plate. Reproducible fabrication of good-quality displays was achieved, with >99 percent of the elements operational. Excellent alphanumeric displays with very few defects were demonstrated. Contrast ratios >50:1 (ON:OFF under dark ambient) were obtained. Power consumption under typical alphanumeric display conditions was below 1 W, with a peak brightness of 40 fL.
Unravelling the Effects of Syndecan-4 Knockdown on Skeletal Muscle Functions (2023)

The remodelling of the extracellular matrix plays an important role in skeletal muscle development and regeneration. Syndecan-4 is a cell surface proteoglycan crucial for muscle differentiation. Syndecan-4<sup>-/-</sup> mice have been reported to be unable to regenerate following muscle damage. To investigate the consequences of the decreased expression of Syndecan-4, we have studied the in vivo and in vitro muscle performance and the excitation-contraction coupling machinery in young and aged Syndecan-4<sup>+/-</sup> (SDC4) mice. In vivo grip force was decreased significantly as well as the average and maximal speed of voluntary running in SDC4 mice, regardless of their age. The maximal in vitro twitch force was reduced in both EDL and soleus muscles from young and aged SDC4 mice. Ca<sup>2+</sup> release from the sarcoplasmic reticulum decreased significantly in the FDB fibres of young SDC4 mice, while its voltage dependence was unchanged regardless of age. These findings were present in muscles from young and aged mice as well. On C2C12 murine skeletal muscle cells, we have also found altered calcium homeostasis upon Syndecan-4 silencing. The decreased expression of Syndecan-4 leads to reduced skeletal muscle performance in mice and altered motility in C2C12 myoblasts via altered calcium homeostasis. The altered muscle force performance develops at an early age and is maintained throughout the life course of the animal until old age.
Mutations in N-terminal Flanking Region of Blue Light-sensing Light-Oxygen and Voltage 2 (LOV2) Domain Disrupt Its Repressive Activity on Kinase Domain in the Chlamydomonas Phototropin (2012)

Phototropin is a light-regulated kinase that mediates a variety of photoresponses such as phototropism, chloroplast positioning, and stomata opening in plants to increase the photosynthetic efficiency. Blue light stimulus first induces local conformational changes in the chromophore-bearing light-oxygen and voltage 2 (LOV2) domain of phototropin, which in turn activates the serine/threonine (Ser/Thr) kinase domain in the C terminus. To examine the kinase activity of full-length phototropin conventionally, we employed the budding yeast Saccharomyces cerevisiae. In this organism, Ser/Thr kinases (Fpk1p and Fpk2p) that show high sequence similarity to the kinase domain of phototropins exist. First, we demonstrated that the phototropin from Chlamydomonas reinhardtii (CrPHOT) could complement loss of Fpk1p and Fpk2p to allow cell growth in yeast. Furthermore, this reaction was blue light-dependent, indicating that CrPHOT was indeed light-activated in yeast cells. We applied this system to a large scale screening for amino acid substitutions in CrPHOT that elevated the kinase activity in darkness. Consequently, we identified a cluster of mutations located in the N-terminal flanking region of LOV2 (R199C, L202L, D203N/G/V, L204P, T207I, and R210H). An in vitro phosphorylation assay confirmed that these mutations substantially reduced the repressive activity of LOV2 on the kinase domain in darkness. Furthermore, biochemical analyses of the representative T207I mutant demonstrated that the mutation affected neither spectral nor multimerization properties of CrPHOT. Hence, the N-terminal flanking region of LOV2, as is the case with the C-terminal flanking Jα region, appears to play a crucial role in the regulation of kinase activity in phototropin.
Environmentally Friendly Mechanochemical Syntheses and Conversions of Highly Luminescent Cu(I) Dinuclear Complexes (2016)

Luminescent dinuclear Cu(I) complexes, [Cu2X2(dpypp)2] [Cu-X; X = Cl, Br, I; dpypp = 2,2'-(phenylphosphinediyl)dipyridine], were successfully synthesized by a solvent-assisted mechanochemical method. A trace amount of the assisting solvent plays a key role in the mechanochemical synthesis; only two solvents possessing the nitrile group, CH3CN and PhCN, were effective for promoting the formation of dinuclear Cu-X. X-ray analysis revealed that the dinuclear structure with no Cu···Cu interactions, bridged by two dpypp ligands, was commonly formed in all Cu-X species. These complexes exhibited bright green emission in the solid state at room temperature (Φ = 0.23, 0.50, and 0.74; λem = 528, 518, and 530 nm for Cu-Cl, Cu-Br, and Cu-I, respectively). Emission decay measurement and TD-DFT calculation suggested that the luminescence of Cu-X could be assigned to phosphorescence from the triplet metal-to-ligand charge-transfer ((3)MLCT) excited state, effectively mixed with the halide-to-ligand charge-transfer ((3)XLCT) excited state, at 77 K. The source of emission changed to thermally activated delayed fluorescence (TADF) with the same electronic transition nature at room temperature. In addition, the CH3CN-bound analogue, [Cu2(CH3CN)2(dpypp)2](BF4)2, was successfully mechanochemically converted to Cu-X by grinding with solid KX in the presence of a trace amount of assisting water.
Taking care of the team in a first-year design experience course (2014)

Design experience coursework for first-year engineering and computer science students is not new. These experiences are often team-based and typically focus extensively on the technical aspects of the project, while the behavioral and interpersonal aspects sometimes go unnoticed. Here, we report on a novel organization for an introduction to engineering design course that provides direct and proactive support for both the engineering components of the course as well as proactive support for first-year students and their teams. The course is structured to allow students to focus on engineering design and implementation of a robotics system while also taking time to engage in reflection activities geared towards assessment of team dynamic and impact on team productivity. Team facilitators observe the teams during work time over the course of the semester and use information gathered in structured team debriefs called retrospectives. While much work has been done, we continue to iterate on the design and implementation of this course.
Versatile Electroanalytical Bioplatforms for Simultaneous Determination of Cancer-Related DNA 5-Methyl- and 5-Hydroxymethyl-Cytosines at Global and Gene-Specific Levels in Human Serum and Tissues (2018)

This paper reports the preparation of versatile electrochemical biosensing platforms for the simple, rapid, and PCR-independent detection of the most frequent DNA methylation marks (5-methylcytosine, 5-mC, and/or 5-hydroxymethylcytosine, 5-hmC) both at global and gene-specific levels. The implemented strategies, relying on the smart coupling of immuno-magnetic beads (MBs), specific DNA probes and amperometric detection at screen-printed carbon electrodes (SPCEs), provided sensitive and selective determination of the target methylated DNAs in less than 90 min with a great reproducibility and demonstrated feasibility for the simultaneous detection of the same or different cytosine epimarks both at global level and in different loci of the same gene or in different genes. The bioplatforms were applied to determine global methylation events in paraffin-embedded colorectal tissues and specific methylation at promoters of tumor suppressor genes in genomic DNA extracted from cancer cells and paraffin-embedded colorectal tissues, and in serum without previous DNA extraction from cancer patients.
Growth of Amorphous SiO2 Nanowires on Si using a Pd/Au Thin Film as a Catalyst (2004)

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.
Process Tomography for Multiphase Flow Analysis (2003)

Besides NDT applications X-ray computed tomography can be applied to the analysis of multiphase flows which are common in process engineering as well as in the power industry. Two kind of process CT systems for multiphase flow analysis are described. 1. A 3D X-ray tomograph with high special resolution and low time resolution which is dedicated to analyzing the 3D gas distribution in vertical bubbly flow. This high resolution proved to be crucial to determine the detailed structure of wall-peaked gas distributions which occur in upward bubbly flow. 2. A 2D X-ray tomograph with high time resolution and low special resolution which is dedicated to visualize the dynamic motion of interfaces in multi-phase flow. It successfully quantified the transient multi-dimensional characteristics of interfaces in the flow.
Selected applications of Rietveld-XRD analysis for raw materials of the aluminum industry (2013)

In the last few decades, X-ray diffraction (XRD) systems have been paramount and irreplaceable in controlling bauxite exploration, as well as Bayer and reduction processes. XRD quantitative phase analysis in the aluminum industry witnessed a steady deployment of the Rietveld method, which at present progressively replaces existing methodologies in research and plant laboratories. Rietveld analysis not only helped to surpass traditional XRD calibration methods, it also opened the door for new applications previously not possible. The use of the Rietveld method to characterize selected materials unique to the aluminum industry, such as bauxite, red mud, and alumina is demonstrated and discussed. This paper also presents how synchrotron-based diffractograms obtained for bauxite and red mud samples allowed a much better understanding of mineralogical representation, and made it possible to leverage their Rietveld quantification. Despite clear advantages, the Rietveld method also has limitations that are revealed. For alumina phase quantification, a dedicated Rietveld analytical program was built with structure data for eight alumina mineralogical phases: alpha, beta ( β -Al 2 O 3 = Na 2 O•11Al 2 O 3 ), delta, gamma (2), kappa, sigma, and theta. The paper gives unique examples of phase quantification in aluminas of various origins and phase composition.
[Changes in bone mass and in glucose homeostasis in subjects with high spontaneous fluoride intake]. (1997)

This paper reports metabolic data of 24 women and two men, 44-66 years old, ex-residents in an area of endemic fluorosis close to Bahía Blanca city. Fasting fluoremias of these subjects (0.5 to 9.2 microM) and daily urinary fluoride excretion (> 60 mumoles/day) are characteristics of zones with endemic fluorosis. Bone mineral density (BMD) at the lumbar spine (L2-4 1330 +/- 41 mg/cm2) and femoral neck (1045 +/- 10 mg/cm2) were significantly above average of normal subjects of the same age and sex. A significant correlation was observed between the daily excretion of fluoride and BMD L2-4 (r = 0.43, P < 0.05). The Area Under the Curve of insulin during a standard glucose tolerance test showed an inverse relationship with fluoremia. This observation coincides with experiments published elsewhere indicating that fluoride intake at concentrations 5 microM or greater, inhibits the secretion of insulin.
A fully homomorphic encryption scheme with better key size (2014)

Fully homomorphic encryption is faced with two problems now. One is candidate fully homomorphic encryption schemes are few. Another is that the efficiency of fully homomorphic encryption is a big question. In this paper, we propose a fully homomorphic encryption scheme based on LWE, which has better key size. Our main contributions are: (1) According to the binary-LWE recently, we choose secret key from binary set and modify the basic encryption scheme proposed in Linder and Peikert in 2010. We propose a fully homomorphic encryption scheme based on the new basic encryption scheme. We analyze the correctness and give the proof of the security of our scheme. The public key, evaluation keys and tensored ciphertext have better size in our scheme. (2) Estimating parameters for fully homomorphic encryption scheme is an important work. We estimate the concert parameters for our scheme. We compare these parameters between our scheme and Bra12 scheme. Our scheme have public key and private key that smaller by a factor of about logq than in Bra12 scheme. Tensored ciphertext in our scheme is smaller by a factor of about log2q than in Bra12 scheme. Key switching matrix in our scheme is smaller by a factor of about log3q than in Bra12 scheme.
Characterization of the surface protein layers of the mosquito-pathogenic strains of Bacillus sphaericus (1987)

The protein surface layers on the cell walls of mosquito-pathogenic and nonpathogenic Bacillus sphaericus strains were studied by structural, biochemical, and serological methods. The surface structure of two representative insect-pathogenic strains had the form of a delicate linear array with a repeat interval of 5 nm. This was distinctly different from the tetragonal array of the P-1 strain in spacing and arrangement. The surface layers were composed of acidic glycoproteins with molecular weights ranging from approximately 133,000 to 155,000. Peptide mapping and serological analysis of the surface proteins revealed eight distinct groups among the pathogens. These groups were very similar to the groupings determined by flagellar-antigen serotyping and bacteriophage typing.
Reproduction of Mucohaemorrhagic Diarrhea and Colitis Indistinguishable from Swine Dysentery following Experimental Inoculation with “Brachyspira hampsonii” Strain 30446 (2013)

"Brachyspira hampsonii" strain 30446 causes mucohaemorrhagic diarrhea in pigs following a 4-9 day incubation period. Fecal shedding was detectable by day 4 post inoculation, and rarely preceded the onset of mucoid or haemorrhagic diarrhea by more than 2 days. Culture and 30446-specific qPCR are reliable methods of detection of this organism in feces and tissues of diarrheic pigs. The emergence of a novel Brachyspira spp., such as "B. hampsonii", creates diagnostic challenges including higher risk of false negative diagnostic tests. We therefore recommend diagnostic laboratories routinely use Brachyspira culture, nox-based and species-specific PCR, and DNA sequencing to diagnose Brachyspira-associated colitis in pigs.
Discussion (2012)

Ann Arbor, Mich. From the Section of Plastic Surgery, University of Michigan Health System. Received for publication May 3, 2012; accepted May 9, 2012. Disclosure:The author has no financial interest to declare in relation to the content of this Discussion or of the associated article. Paul S. Cederna, M.D.; Section of Plastic Surgery, University of Michigan Health System, 2130 Taubman Center, 1500 East Medical Center Drive, Ann Arbor, Mich. 48109-0340, [email protected]
Enhancement of Tumor Oxygenation and Radiation Response by the Allosteric Effector of Hemoglobin, RSR13 (2001)

Prior studies using pO(2) microelectrodes have shown that RSR13, an allosteric modifier of hemoglobin, increases tissue oxygenation in vivo. Recently, measurements of tissue oxygenation have been performed by many investigators using blood oxygen level-dependent magnetic resonance imaging (BOLD MRI). In this study, we tested the hypothesis that the BOLD MRI signal ratio in tumors will change after administration of RSR13. NCI-H460 human lung carcinoma cells were used as a xenograft in athymic nude mice. Mice with 1-cm(3) tumors in the flank were anesthetized and mounted on the MRI apparatus, and various doses of RSR13 were administered intraperitoneally (i.p.). MR images were then acquired at 10-min intervals for up to 60 min after injection. The effect of RSR13 on tumor response was studied using the same mouse xenograft model with tumor growth delay measurements. RSR13 increased the MRI signal ratio [Intensity(t)/Intensity(t = 0)] in a dose-dependent manner, with maximum increases occurring 30 min after RSR13 was administered. An RSR13 dose of 200 mg/kg proved to be optimum. Since the MRI signal ratio has been shown previously to be linearly related to tissue oxygenation, the changes in the MRI signal ratio can be attributed to changes in tumor oxygen levels. Using a 200-mg/kg dose of RSR13, with a 10-Gy dose of radiation administered to tumors 30 min later, enhancement of radiation-induced tumor growth delay by RSR13 was observed (enhancement factor = 2.8). Thus our MRI results support and verify the previously reported RSR13-induced increase in tumor oxygenation obtained using pO(2) microelectrodes. Based upon these results and other previous studies, the mechanism of enhancement of the effect of radiation by RSR13 probably involves an increase in tumor oxygenation.
Efficient C−B Bond Formation Promoted by N-Heterocyclic Carbenes: Synthesis of Tertiary and Quaternary B-Substituted Carbons through Metal-Free Catalytic Boron Conjugate Additions to Cyclic and Acyclic α,β-Unsaturated Carbonyls (2010)

Efficient C−B Bond Formation Promoted by N-Heterocyclic Carbenes: Synthesis of Tertiary and Quaternary B-Substituted Carbons through Metal-Free Catalytic Boron Conjugate Additions to Cyclic and Acyclic α,β-Unsaturated Carbonyls
Regulation of cAMP-responsive enhancer binding proteins during cell cycle progression in T lymphocytes stimulated by IL-2. (1995)

IL-2 stimulates the proliferative response of various lymphoid cells. Previous studies showed an increase in intracellular levels of cAMP concomitant with an increase in phosphorylation of discrete proteins by protein kinase A at late G1 phase in mitogen-stimulated lymphocytes. Thus, experiments were undertaken to study nuclear proteins that bind to the cAMP-responsive enhancer (CRE) in cloned T lymphocytes stimulated with IL-2. With the use of a 32P-labeled CRE consensus sequence in a DNA binding gel mobility shift assay, we showed that IL-2 stimulation resulted in the induction of two major DNA-protein complexes at late G1/S during the cell cycle. This binding was competed in a dose-dependent manner by a nonlabeled CRE oligonucleotide but was not competed by a nonlabeled AP-1 oligonucleotide. Rapamycin, a potent immunosuppressant, which arrests IL-2-stimulated T lymphocytes at G1/S, inhibited the IL-2-induced CRE binding activities concomitantly with inhibition of DNA synthesis. By using specific Abs in a gel mobility shift assay, we identified two known CREB/ATF transcription factors in the IL-2-induced CRE complexes: the CRE binding factor (CREB), and ATF1. The induction of CREB binding by IL-2 was not associated with an increase in its abundance but was associated with a major increase in CREB phosphorylation that was particularly prominent at late G1/S. However, we found that G1/S progression induced by IL-2 was not associated with an increase in the intracellular levels of cAMP. These results suggest that 1) the transcription factors CREB and ATF1 and possibly other CRE binding proteins may have an important role in the modulation of specific gene expression at G1/S during cell cycle progression induced by IL-2. 2) The involvement of these CRE binding transcription factors in IL-2-stimulated cells is regulated via a mechanism that is not cAMP dependent.
Older adults with complex care needs’ experiences within a hospital-to-home transition patient navigator program: A qualitative descriptive study (2023)

Introduction Older adults living with complex care needs are often hospitalized due to poor community support. Recommendations for improving patient and family experiences include having a patient navigator to support patients and caregivers in hospital and community settings. This study aimed to report on older adults’ and caregivers’ experiences of receiving services from a hospital-to-home patient navigation program. Methods A qualitative descriptive study was conducted. Telephone interviews were conducted with 14 older adults with complex care needs or their family caregivers in Toronto, Ontario from 2020 to 2021. Data were analyzed using inductive thematic analysis. Results Four themes were identified: (1) Initial Hesitancy; (2) Meeting Evolving Needs; (3) Unexpected Benefits of Patient Navigation; and (4) The Value of Patient Navigation in Sustaining Aging-in-Place. Participants viewed all their interactions with the navigator as positive, and perceived navigators improved their quality of care. Discussion These study findings suggest that patient navigators may be well-positioned to address gaps in services around psychosocial support and care coordination while also encouraging self-management in older adults with complex care needs.
Identification of human lymphoma cells by antisera to malignancy- associated nucleolar antigens (1984)

The non-Hodgkin's lymphomas (NHL) are a diverse group of human lymphoid neoplasms that have long presented pathologists with formidable diagnostic challenges. These tumors of the immune system are thought to represent neoplastic transformations of most of the recognized stages in T and B lymphocyte ontogeny. Lymphoma cells, however, often simulate their normal lymphocytic counterparts both morphologically and cell surface phenotypically, creating difficulties in discriminating normal from neoplastic lymphocytes. We have used heteroantisera to the human malignancy-associated nucleolar antigen (HMNA) to prospectively evaluate its efficacy in identifying the morphologically neoplastic cells in NHL lesions. In 65 cases of T and B cell histopathologic types of NHL, the antisera reacted with nucleoli in the morphologically and cytogenetically neoplastic lymphoma cells, but not with normal- appearing lymphoid and other cell types present in the lesions. Control specimens from normal and hyperplastic lymphoid tissue also failed to react with anti-HMNA antibodies. Normal activated lymphoid cells in vitro and growth-factor-dependent normal lymphoid cell lines also failed to express the nucleolar antigen(s). These data suggest that the HMNA is a valuable tumor cell marker for neoplastic human lymphoid cell populations and can be used with other types of cell markers for a better definition of the neoplastic cells in NHL.
Modelling of non-thermal plasma aftertreatment of exhaust gas streams (2003)

A reaction mechanism has been developed that is appropriate for the plasma aftertreatment of diesel exhaust gas. It is based on a simulated gas mixture containing propene, nitric oxide, nitrogen dioxide, oxygen and nitrogen. The reaction mechanism has been used to determine the end-products from the plasma processing and their concentrations using a chemical kinetics modelling procedure. It has been validated by a range of experiments using the same gas mixture with a packed bed, a dielectric barrier plasma reactor and a wide range of end-product analysis techniques. Using a wide range of experimental conditions has enabled us to validate the model and its predictions and to critically evaluate several alternative reaction mechanisms for the oxidation of propene and the formation of end-products in a more systematic and reliable manner than before.