gebhart/patrepeval huggingface dataset:
Impaired PIASy–Tip60 signaling weakens activation of p53 in melanoma (2013)

The tumor suppressor p53 plays a central role in preventing tumor development by promoting transcription of genes that stall cell cycle and induce cell death. Although the majority of melanomas express wild-type p53, the molecular mechanisms that impede its activation remain unclear. We previously reported that the SUMO E3 ligase PIASy and the histone acetyltransferase Tip60 signaling cascade promote p53-dependent autophagy and apoptosis. We hypothesized that impairment in this signaling attenuates p53, thus disabling its apoptotic function in melanoma. Here, we show that human melanoma patient samples and cell lines maintain p53 expression but PIASy and/or Tip60 are frequently lost. We observed dysregulation of Tip60-mediated p53 transcription program in melanoma cell lines. Reconstitution of PIASy and Tip60 in melanoma cells increased genotoxic stress-induced apoptosis. Our study provides a clinical link of how sumoylation signaling may activate p53-mediated cell death in melanoma.
Counteracting in Vitro Toxicity of the Ionophoric Mycotoxin Beauvericin—Synthetic Receptors to the Rescue (2019)

Beauvericin (BEA) and enniatins are toxic ionophoric cyclodepsipeptides that mainly occur in grains. As such, their presence in food commodities poses a concern for public health. To date, despite recent European Food Safety Authority emphasis on the need for more data to evaluate long-term toxicity effects, no suitable affinity reagents are available to detect the presence of BEA and derivatives in food samples. We here report on the synthesis of a small library of artificial receptors with varying cavity sizes and different hydrophobic building blocks. Immobilization of one of the receptors on solid support resulted in a strong retention of beauvericin, thus revealing promising properties as solid-phase extraction material for sample pretreatment. Furthermore, treatment of HepG2 cells with the most promising receptor markedly reduced beauvericin-induced cytotoxicity, hinting toward the possibility of using synthetic receptors as antidotes against ionophoric toxins.
A New Palladium-Catalyzed P-C Coupling Reaction: Synthesis of Triarylphosphine Oxides and Diarylmethylphosphine Oxides (2000)

Abstract The first palladium-catalyzed P-C coupling reaction of aryl halides with (hydroxymethyl)phosphines is described. Hydroxymethylphosphines act as cheap and easy to use H3PO and CH3P(O)H2 equivalents. Tertiary diarylmethylphosphine oxides 5 and triarylphosphine oxides 6 are accessible in a one pot reaction in overall yields between 45-60%.
Synthesis of Carbazoles by a Merged Visible Light Photoredox and Palladium-Catalyzed Process (2015)

Carbazoles have attracted great interest in recent years for a variety of applications in organic and medicinal chemistry as well as in materials science. In this work, an efficient method for the synthesis of carbazoles through the intramolecular C–H bond amination of N-substituted 2-amidobiaryls has been developed. Under visible light and an aerobic atmosphere, the transformation requires only catalytic amounts of Pd(OAc)2 and [Ir(dFppy)2phen]PF6 (dFppy = 2-(2,4-difluorophenyl)pyridine; phen = 1,10-phenanthroline), the latter of which is utilized in synthetic chemistry for the first time. Spectroscopic and electrochemical studies revealed that the reaction is initiated by photoinduced electron transfer from a palladacyclic intermediate, formed from the 2-amidobiaryl and PdII species, to the photoexcited Ir catalyst. This step triggers reductive elimination in a PdIII-containing palladacycle to produce the carbazole and a PdI species. The one-electron-reduced photocatalyst is reoxidized by O2 to generate the original form of the photocatalyst, and the PdI species can be oxidized to the resting state through oxidative electron transfer to O2 or the excited-state photocatalyst.
Whitening effect of flavonoid materials in Radix puerariae (2013)

Mechanism for whitening effect of flavonoid materials in Radix puerariae was studied via investigation of its inhibition action on tyrosinase and antioxidizing action.Laboratory test results showed that the inhibition effect of flavonoid materials in Radix puerariae on tyrosinase is stronger than that of arbutin and its concentration leading to 50% activity lost(IC50) was identified as 1.72 g·L-1.The flavonoid materials in Radix puerariae displays a rather strong overall reducing action capacity and certain scavenging action capacity against superoxide radicals,hydroxyl radicals as well as diphenyl-picryl hydrazyl(DPPH) radicals.IC50 for scavenging superoxide radicals and DPPH radicals achieves 0.98 and 0.81 g·L-1 respectively.
An electron spin resonance investigation on intermediates and products in the basic oxidation of nitrodiphenylethylenes (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn electron spin resonance investigation on intermediates and products in the basic oxidation of nitrodiphenylethylenesM. Barzaghi, P. L. Beltrame, A. Gamba, and M. SimonettaCite this: J. Am. Chem. Soc. 1978, 100, 1, 251–259Publication Date (Print):January 1, 1978Publication History Published online1 May 2002Published inissue 1 January 1978https://doi.org/10.1021/ja00469a042RIGHTS & PERMISSIONSArticle Views66Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (922 KB) Get e-Alerts Get e-Alerts
Development of a vendor agnostic, full disclosure system for capture, display, and storage of operative systems data. (2006)

We have developed a vendor agnostic, full disclosure system for the capture, display, and storage of operative systems data. This system allows door to door capture of data from multiple sources including monitors from competing vendors, integration under a single platform, and storage for future use. Full disclosure functionality includes the ability to retrieve and display archived data including full waveform and trend physiologic data, synchronized to the surgical video and other OR devices and data sources.
Quantifying High Temperature Corrosion (2021)

Abstract Alloys designed for high temperature service can form a variety of surface oxides or scales based on the alloy composition, service temperature, gas environment, surface deposits, etc. The most severe environments, including molten salts and liquid metals can result in significant metal loss, void formation and/or pitting. Standard and evolving practices are discussed for measuring these types of degradation including measuring oxide scale thickness, internal oxidation and/or metal loss, correlating them to mass change and reporting the results. Manual and automated methodologies to measure oxide thickness and metal loss are reviewed and compared in order to provide a summary of techniques and identify the best practices for quantifying a variety of materials and damage mechanisms and producing statistically meaningful results. Such techniques create datasets useful for improved understanding of corrosion degradation mechanisms, potentially better predictive models and enhanced data analytics.
An Expert Smart Scalp Inspection System Using Deep Learning (2022)

With the advent of the "beauty economic era", in which people are paying more attention to beauty and health, the health of the scalp is being increasingly valued. However, current scalp care services are limited by problems such as they are not automatic and objective, and the results are not significant, which make them unacceptable to the public. Because of these reasons, in this study, we focus on the obstacles that hairdressers face and propose an expert inspection system that is suitable for determining scalp problems by utilizing deep learning, cloud computing techniques, and an embedded system. Dandruff is the most common scalp problem. In this work, we propose a convolutional neural network (CNN)-based method to analyze the severity of dandruff and evaluate the health of the scalp. The convolutional block attention module (CBAM) is adopted to improve the feature extraction performance of the CNN model. The depth separable convolution (DSC) and spinal fully connected (FC) are applied in this work to reduce the number of model parameters. Aside from offering a more effective smart scalp inspection process, this method also enables hairdressers and customers to track their scalp problems easily. In the future, we expect to reduce the stress of hairdressers and enhance customers' trust on scalp care services by using the smart health inspection offered by this system. Last but not the least, it has been shown that the method proposed in this research can achieve an accuracy of 85.03%, which is higher than those achieved by recently proposed methods.
A new Al0.3Ga0.7As/GaAs modulation-doped FET (1982)

A new Al <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.3</inf> Ga <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.7</inf> As/GaAs modulation-doped FET fabricated like a MESFET but operating like a JFET was successfully fabricated and tested. This new device replaces the Schottky gate of the MESFET with an n+/p+ camel diode structure, thereby allowing problems associated with the former to be overcome. The devices, which were fabricated from structures grown by molecular beam epitaxy (MBE), had a 1µm gate length, a 290µm gate width, and a 4µm channel length. The room temperature transconductance normalized to the gate width was about 95 mS/mm, which is comparable to that obtained in similar modulation-doped Schottky barrier FET's. Unlike modulation-doped Schottky barrier FET's, fabrication of this new device does not require any critical etching steps or formation of a rectifying metal contact to the rapidly oxidizing Al <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.3</inf> Ga <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.7</inf> As. Relatively simple fabrication procedures combined with good device performance make this camel gate FET suitable for LSI applications.
Colloidal nanoparticle clusters: functional materials by design (2012)

Significant advances in colloidal synthesis made in the past two decades have enabled the preparation of high quality nanoparticles with well-controlled sizes, shapes, and compositions. It has recently been realized that such nanoparticles can be utilized as 'artificial atoms' for building new materials which not only combine the size- and shape-dependent properties of individual nanoparticles but also create new collective properties by taking advantage of their electromagnetic interactions. The controlled clustering of nanoparticle building blocks into defined geometric arrangements opens a new research area in materials science and as a result much interest has been paid to the creation of secondary structures of nanoparticles, either by direct solution growth or self-assembly methods. In this tutorial review, we introduce recently developed strategies for the creation and surface modification of colloidal nanoparticle clusters, demonstrate the new collective properties resulting from their secondary structures, and highlight several of their many important technological applications ranging from photonics, separation, and detection, to multimodal imaging, energy storage and transformation, and catalysis.
Comparison of the Production of Charged Hadrons at Large Transverse Momentum inπ−pandppCollisions (1981)

Measurements of the production cross sections at large values of transverse momentum (${P}_{T}$) for ${\ensuremath{\pi}}^{\ifmmode\pm\else\textpm\fi{}}$, ${K}^{\ifmmode\pm\else\textpm\fi{}}$, $p$, and $\overline{p}$ in 200- and 300-GeV/c ${\ensuremath{\pi}}^{\ensuremath{-}}p$ collisions are presented. The dependences of the cross section on ${P}_{T}$, the transverse scaling variable ${x}_{T}=\frac{2{P}_{T}}{\sqrt{s}}$, and the production angle are discussed. The cross sections are compared with those from $\mathrm{pp}$ collisions and with theoretical predictions. While the ${\ensuremath{\pi}}^{\ensuremath{-}}p$ and $\mathrm{pp}$ data are quite different, both agree qualitatively with hard-scattering calculations.
EFFECTS OF CEPHALOSPORIN ANTIBIOTICS ON THE CONTRACTILE RESPONSE OF GUINEA-PIG URINARY BLADDER (1985)

The present study was undertaken to investigate the effects of cephalothin(CET), cephalexin(CEX),and 7-aminocephalosporanic acid(7-ACA)on the contractile response of guinea-pig urinary bladder. CET,CEX,and 7-ACA in the concentration of 1x10-4g/m1-1x10-4g/m1 had no effect on the transmural electrical stimulation-induced contractile responses.These agents in the concentration of 1x10-8g/m1 slightly reduced them as 97•}3%(n=12),97
Experimental evaluation of remote beamforming scheme with fixed wavelength allocation in IF-over-fiber systems (2021)

Radio over fiber (RoF) technology, especially intermediate frequency over fiber (IFoF), is attracting much attention as one way to realize high frequency band systems. Such systems require beamforming to attain the desired link budget. Furthermore, highly accurate beamforming permits the adoption of space division multiplexing (SDM), with its greater system capacity. Since beamforming should be controlled by the central station to achieve simple base stations, we proposed a novel remote beamforming scheme with fixed wavelength allocation. This paper introduces experiments conducted on modulated signals to evaluate the characteristics of the scheme’s beamforming gain and signal to interference ratio (SIR).
A Novel Full-Length Recombinant Human Complement Factor H (CFH; GEM103) for the Treatment of Age-Related Macular Degeneration Shows Similar In Vitro Functional Activity to Native CFH (2022)

GEM103, a recombinant CFH developed by Gemini Therapeutics, shows activity profiles comparable to sdCFH in all complement-related assays employed in this study, suggesting that GEM103 is equivalent to the native glycoprotein in terms of its <i>in vitro</i> functional activity. These results support further study of GEM103 as a potential therapy for AMD.
Role of Pseudomonas putida tol-oprL Gene Products in Uptake of Solutes through the Cytoplasmic Membrane (2003)

Proteins of the Tol-Pal (Tol-OprL) system play a key role in the maintenance of outer membrane integrity and cell morphology in gram-negative bacteria. Here we describe an additional role for this system in the transport of various carbon sources across the cytoplasmic membrane. Growth of Pseudomonas putida tol-oprL mutant strains in minimal medium with glycerol, fructose, or arginine was impaired, and the growth rate with succinate, proline, or sucrose as the carbon source was lower than the growth rate of the parental strain. Assays with radiolabeled substrates revealed that the rates of uptake of these compounds by mutant cells were lower than the rates of uptake by the wild-type strain. The pattern and amount of outer membrane protein in the P. putida tol-oprL mutants were not changed, suggesting that the transport defect was not in the outer membrane. Consistently, the uptake of radiolabeled glucose and glycerol in spheroplasts was defective in the P. putida tol-oprL mutant strains, suggesting that there was a defect at the cytoplasmic membrane level. Generation of a proton motive force appeared to be unaffected in these mutants. To rule out the possibility that the uptake defect was due to a lack of specific transporter proteins, the PutP symporter was overproduced, but this overproduction did not enhance proline uptake in the tol-oprL mutants. These results suggest that the Tol-OprL system is necessary for appropriate functioning of certain uptake systems at the level of the cytoplasmic membrane.
Identification of RL-TGR, a coreceptor involved in aversive chemical signaling (2010)

Chemical signaling plays an important role in predator-prey interactions and feeding dynamics. Like other organisms that are sessile or slow moving, some marine sponges contain aversive compounds that defend these organisms from predation. We sought to identify and characterize a fish chemoreceptor that detects one of these compounds. Using expression cloning in Xenopus oocytes coexpressing the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel, the beta-2 adrenergic receptor (beta(2)AR), and fractions of a zebrafish cDNA library, we isolated a cDNA clone encoding receptor activity-modifying protein (RAMP)-like triterpene glycoside receptor (RL-TGR), a novel coreceptor involved in signaling in response to triterpene glycosides. This coreceptor appears to be structurally and functionally related to RAMPs, a family of coreceptors that physically associate with and modify the activity of G protein-coupled receptors (GPCRs). In membranes from formoside-responsive oocytes, RL-TGR was immunoprecipitated in an apparent complex with beta(2)AR. In HEK293 cells, coexpression of beta(2)AR induced the trafficking of RL-TGR from the cytoplasm to the plasma membrane. These results suggest that RL-TGR in the predatory fish physically associates with the beta(2)AR or another, more physiologically relevant GPCR and modifies its pharmacology to respond to triterpene glycosides found in sponges that serve as a potential food source for the fish. RL-TGR forms a coreceptor that responds to a chemical defense compound in the marine environment, and its discovery might lead the way to the identification of other receptors that mediate chemical defense signaling.
Abstract CN06-04: Combination strategies (2015)

Abstract Targeting the endocrine axis remains a mainstay of the treatment of many breast and most prostate cancers. Recent studies indicate that these tumors commonly remain nuclear steroid hormone receptor driven once endocrine resistance ensues. Multiple novel, rationally designed, agents are in development that are showing promise in the treatment of these diseases including drugs targeting p110beta, AKT, TORC1/2, as well as drugs targeting PARP, bromodomain proteins BRD2-4 and heat shock proteins including HSP90 and HSP27. Furthermore novel agents targeting the amino terminus of the androgen receptor and degrading these receptors are being evaluated. This presentation will describe challenges and solutions to their successful drug combination development. Citation Format: Johann S. De Bono. Combination strategies. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2015 Nov 5-9; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2015;14(12 Suppl 2):Abstract nr CN06-04.
Output characteristics of a neodymium laser with a positive electrooptic feedback (1984)

An investigation was made of the output parameters of a neodymium laser with a positive electrooptic feedback (PEF). Comparison was made of the kinetics of the laser radiation field in three cases: conventional Q switching, PEF regime, Q switching by a crystal of LiF(F2−). It was found that the kinetics of the laser radiation field in the PEF regime was close to the kinetics obtained using the LiF(F2−) switch. In contrast to a laser with a passive filter characterized by a random change of lasing filaments, the structure of the radiation field in the PEF regime remained practically constant during the generation of a giant pulse. The dependence of the operation of the PEF laser on the control voltages could be utilized in computer control of the laser.
Identification and elucidation of proline‐rich antimicrobial peptides with enhanced potency and delivery (2019)

Proline-rich antimicrobial peptides (PrAMPs) kill bacteria via a nonlytic mechanism in which they permeate through the outer membrane, utilize protein-mediated transport across the inner membrane, and target the ribosome to inhibit protein synthesis. We previously reported that substitutions of oncocin ( <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msub><mml:mrow><mml:mtext>VDKPPYLPRPRPPRRIYNR</mml:mtext><mml:mo>-</mml:mo><mml:mtext>NH</mml:mtext></mml:mrow><mml:mn>2</mml:mn></mml:msub></mml:math> ) with a pair of cationic residues improved the antimicrobial activity. In this study, we applied the design protocol to three other PrAMPs: apidaecin-1b, pyrrhocoricin, and bactenecin 7(1-16) and found that the substitutions (R4K and I8K/R) for apidaecin-1b improve the activity by twofold (p < .05) against nonpathogenic Escherichia coli. Moreover, the substitutions (L7K/R and R14K) for pyrrhocoricin improve the activity by 2-10-fold (p < .05) against some strains of E. coli and Salmonella Typhimurium. We also performed activity tests against inner membrane protein (SbmA or YgdD) knockout strains. The result is consistent with previous studies that SbmA is the major transporter for apidaecin-1b and pyrrhocoricin derivatives. However, bactenecin 7(1-16) functions independently of these transporters. In addition, several apidaecin-1b derivatives exhibit enhanced activity relative to wild-type only in the absence of SbmA, which is consistent with mutations that enhance transport across the inner membrane. A high performance liquid chromatography-based kinetic assay for cellular association and internalization demonstrates that the selected cationic mutations can improve cellular association in minimal media, but this enhanced association is not required for increased activity, which suggests the importance of inner membrane transport. These functional studies on cationic mutants of PrAMPs advance understanding of potency and mechanism and advance the ability to engineer improved antimicrobials as evidenced by the identification of the pyrrhocoricin mutant (L7R and R14K) with 10-fold elevated potency against pathogenic E. coli.