gebhart/patrepeval huggingface dataset:
Mitochondrially targeted ZFN s for selective degradation of pathogenic mitochondrial genomes bearing large‐scale deletions or point mutations (2014)

We designed and engineered mitochondrially targeted obligate heterodimeric zinc finger nucleases (mtZFNs) for site-specific elimination of pathogenic human mitochondrial DNA (mtDNA). We used mtZFNs to target and cleave mtDNA harbouring the m.8993T>G point mutation associated with neuropathy, ataxia, retinitis pigmentosa (NARP) and the "common deletion" (CD), a 4977-bp repeat-flanked deletion associated with adult-onset chronic progressive external ophthalmoplegia and, less frequently, Kearns-Sayre and Pearson's marrow pancreas syndromes. Expression of mtZFNs led to a reduction in mutant mtDNA haplotype load, and subsequent repopulation of wild-type mtDNA restored mitochondrial respiratory function in a CD cybrid cell model. This study constitutes proof-of-principle that, through heteroplasmy manipulation, delivery of site-specific nuclease activity to mitochondria can alleviate a severe biochemical phenotype in primary mitochondrial disease arising from deleted mtDNA species.
Bioactive glass‐containing hydrogel delivery system for osteogenic differentiation of human dental pulp stem cells (2019)

Osteoconductive hydrogels can be fabricated by incorporating necessary growth factors and bioactive particles or simply utilizing the ability of the hydrogel itself to induce bone regeneration. The osteogenic inductive potential of the bioactive glass microparticles (BG MPs) has been well-studied. However, the role of the hydrogel embedding the BG MPs on the osteogenic differentiation of the encapsulated stem cells has not been well established. Moreover, it has been reported that the dental pulp stem cell (DPSC) has the capability of regenerating the craniofacial bone tissue when receiving the necessary osteogenic signals from the microenvironment. In the current study, we have fabricated a composite hydrogel based on alginate and Matrigel containing BG MPs and evaluated the role of the BG MPs and Matrigel on osteogenic differentiation of the encapsulated DPSCs. Our results confirmed that presence of Matrigel enhances the osteogenic differentiation of the DPSCs regardless of the decrease in elasticity of the hydrogel in presence of the BG MPs. This strategy of modifying the microenvironment can be a promising approach for craniofacial bone tissue engineering.
Decorin Reduces Intraocular Pressure and Retinal Ganglion Cell Loss in Rodents Through Fibrolysis of the Scarred Trabecular Meshwork (2015)

Human recombinant Decorin reversed established TM fibrosis and lowered IOP, thereby rescuing RGC from progressive death. These data provide evidence for the candidacy of hrDecorin as a treatment for open-angle glaucoma.
Improvement of Interpixel Uniformity in Carbon Nanotube Field Emission Display by Luminance Correction Circuit (2008)

Interpixel uniformity is critical to image quality, and is hard to be improved by structural reformation of emissive elements because of nonuniformity in luminance characteristics of subpixels in self-emissive device like the carbon nanotube field emission display (CNT-FED). In this paper, we discuss the improvement of the interpixel uniformity by individually controlling the luminance of all subpixels in a display panel. We propose a prototype CNT-FED with improved interpixel uniformity using luminance correction circuitry. The analysis of interpixel uniformity with the proposed luminance correction circuit shows that the index of interpixel uniformity increases about 8% with only about 10% luminance reduction ratio that is a relatively small penalty compared to the enhancement of the interpixel uniformity. The proposed correction method can also be used to ensure improvement of the interpixel uniformity in large-size CNT-FED panels.
Classification Decision of Mechanical Drive Types Using LVQ Neural Classifier in Conceptual Design (2011)

In conceptual design of mechanical system, selecting an appropriate mechanical drive type that meets design requirements is a critical problem. To tackle it, a novel classification decision approach to drive type selection is presented, which employs LVQ neural network as classifier to recognize a satisfactory drive from a range of drive types. This recognition process includes five stages: (1) capturing linguistic expression values of character factor for typical drives; (2) digitizing linguistic expression values and extracting features of drives; (3) constructing the structure of a LVQ neural network; (4) training this network with the feature data of character factor set; (5) identifying an appropriate drive type according to the input data using the trained neural network. This proposed approach capitalizes on the benefits of LVQ neural network to acquire domain knowledge and implement classification and decision. Hence at some extent the process of conceptual design can be simplified and the rationality of the decision can be greatly improved.
Role of the ratio of biopolyelectrolyte persistence length to nanoparticle size in the structural tuning of electrostatic complexes (2016)

Aggregation of nanoparticles of given size R induced by addition of a polymer strongly depends on its degree of rigidity. This is shown here on a large variety of silica nanoparticle self-assemblies obtained by electrostatic complexation with carefully selected oppositely charged biopolyelectrolytes of different rigidity. The effective rigidity is quantified by the total persistence length L_{T} representing the sum of the intrinsic (L_{p}) and electrostatic (L_{e}) polyelectrolyte persistence length, which depends on the screening, i.e., on ionic strength due to counterions and external salt concentrations. We experimentally show that the ratio L_{T}/R is the main tuning parameter that controls the fractal dimension D_{f} of the nanoparticles' self-assemblies, which is determined using small-angle neutron scattering: (i) For L_{T}/R<0.3 (obtained with flexible poly-l-lysine in the presence of an excess of salt), chain flexibility promotes easy wrapping around nanoparticles in excess, hence ramified structures with D_{f}∼2. (ii) For 0.3<L_{T}/R≤1 (semiflexible chitosan or hyaluronan complexes), chain stiffness promotes the formation of one-dimensional nanorods (in excess of nanoparticles), in good agreement with computer simulations. (iii) For L_{T}/R>1,L_{e} is strongly increased due to the absence of salt and repulsions between nanoparticles cannot be compensated for by the polyelectrolyte wrapping, which allows a spacing between nanoparticles and the formation of one-dimensional pearl necklace complexes. (iv) Finally, electrostatic screening, i.e., ionic strength, turned out to be a reliable way of controlling D_{f} and the phase diagram behavior. It finely tunes the short-range interparticle potential, resulting in larger fractal dimensions at higher ionic strength.
Differences in airway reactivity in normal and allergic sheep after exposure to sulfur dioxide (1981)

The effect of breathing 5 ppm sulfur dioxide (SO2) on airway reactivity was studied in both normal and allergic conscious sheep. Allergic sheep were defined as animals in which inhalation of Ascaris suum extract resulted in bronchospasm as evidenced by an increase in mean pulmonary flow resistance (RL), hyperinflation, and a fall in dynamic compliance. Airway reactivity was assessed by measuring the increase of RL after 18 breaths of 0.25% carbachol (c), from an initial RL value obtained after 18 breaths of buffered saline (s) [RL(c-s)]. RL and RL(c-s) were determined prior to, immediately after, and 24 h after exposure to 5 ppm SO4 for 4 h. In both groups RL remained unchanged after SO2 exposure. Prior to exposure, RL(c-s) was not significantly different in seven normal (0.3 +/- 0.1) and seven allergic sheep [0.4 +/- 0.2 (SD) cmH2O X l-1 X s], and there was no significant change in RL (c-s) immediately after SO2 exposure in either group. Twenty-four h later, RL(c-s) RL(c-s) increased to 0.7 +/- 0.8 (P less than 0.2) in normal and to 1.8 +/- 0.9 cmH2O X l-1 X s (P less than 0.01) in allergic sheep. Because the increase in RL(c-s) after 24 h was greater (P less than 0.01) in allergic than in normal sheep, we conclude that SO2 exposure increased airway reactivity more in the former than in the latter.
Magnetic, electronic, and thermal properties of buckled kagome Fe3Ge2Sb (2023)

The magnetic, electronic, and thermal properties of ${\mathrm{Fe}}_{3}{\mathrm{Ge}}_{2}\mathrm{Sb}$ single crystals, a derivative of the hexagonal FeGe structure with a buckled Fe kagome net and Sb-Sb dimers are reported. Electronic structure calculations show most of the kagome-derived bands remain intact with the Fe buckling, with the exception of a van Hove singularity near the Fermi level, which is selectively destroyed. This selective destruction of the van Hove singularity is associated with the lack of a charge order transition in ${\mathrm{Fe}}_{3}{\mathrm{Ge}}_{2}\mathrm{Sb}$ compared to FeGe. Magnetization measurements show two antiferromagnetic transition at 290 K and 16 K. The low-temperature transition is attributed to spin canting and is associated with a metamagnetic transition observed in the isothermal magnetization below the transition temperature. Electrical and thermal transport measurements show metallic behavior, and more significant magnetic scattering associated with the metamagnetic transition is observed in the magnetothermal conductivity compared to the magnetoresistance. This is consistent with a modification of the long-period magnetic structure that modifies preferentially small angle scattering thus having a strong impact on thermal transport properties. We conclude that buckled ${\mathrm{Fe}}_{3}{\mathrm{Ge}}_{2}\mathrm{Sb}$ exhibits similar properties to unbuckled hexagonal FeGe with the exception of the lack of a charge density wave transition in ${\mathrm{Fe}}_{3}{\mathrm{Ge}}_{2}\mathrm{Sb}$, likely due to the selective destruction of the van Hove singularity near ${\mathrm{E}}_{F}$, making the family of compounds ${\mathrm{Fe}}_{3}{\mathrm{Ge}}_{3\ensuremath{-}x}{\mathrm{Sb}}_{x}$ a good host to study various physical effects in kagome metals, especially the electronic and structural stabilization of charge ordered states.
Wire-guided Endobronchial Blockade: An Alternative Means For Achieving One-lung Ventilation (2000)

The goal of this presentation is to introduce a new method of one-lung ventilation using a wire guided endobronchial blocker. Ten key points in using this device are described.
Defining Three Distribution System Scenarios for Microgrid Applications (2020)

Power systems, especially distribution systems, are undergoing the most drastic overhauls with the growing integration of renewable energy and digitalization. One of the most efficient technological solutions to address the challenges the distribution system faces today, is the formation of microgrids. With heterogeneous driving forces from policy, regulation, system operation, infrastructure developer, aggregator, and end-user, how microgrid will evolve and develop in the future distribution system remains an open question and deserve closer scrutiny. In this paper, a brief state of the art of current microgrid design is introduced considering knowledge and experience from both practitioner and academia. Based on a simple foresight method, three foreseeable scenarios for the future distribution system are depicted. Aspects related to its use cases, energy management system features, and market models will be discussed for each possible scenario. This will shed light onthe future research and development of microgrid applications.
IL‐15 mediates antigen‐induced neutrophil migration by triggering IL‐18 production (2007)

We have investigated the mechanisms underlying IL-15-induced neutrophil migration into inflamed tissues. IL-15 induced neutrophil migration to the peritoneal cavity in mice in a time- and dose-dependent manner. The cell migration was not induced in IL-18-/-, MIP-1alpha (CCL3)-/-, TNFR1-/- or 5-LOX-/- mice but was normal in IFN-gamma-/- mice. IL-15-induced neutrophil migration was inhibited by anti-MIP-2 (CXCL2) antibody or MK886 (leukotriene synthesis inhibitor). IL-18-induced neutrophil migration was also dependent on TNFR1, MIP-1alpha, MIP-2 and leukotriene. Consistent with this observation, IL-15 induced IL-18 production, and IL-15 or IL-18 injection induced the production of MIP-2, MIP-1alpha, TNF-alpha and LTB4. In an antigen-specific inflammation model, ovalbumin (OVA)-induced neutrophil migration was completely inhibited by soluble IL-15Ralpha (sIL-15Ralpha) or anti-MIP-2 antibody. Furthermore, cell migration was absent in IL-18-/-, MIP-1alpha-/-, TNFR1-/-, or 5-LOX-/- mice. OVA challenge induced the release of MIP-2, MIP-1alpha, TNF-alpha and LTB4 in the peritoneal cavity in an IL-15- and IL-18-dependent manner. We also found that neutrophils from the peripheral blood and synovial fluid of patients with rheumatoid arthritis produced substantial amounts of IL-18 and LTB4 following activation by IL-15. Together, these results demonstrate that IL-15 plays an important role in antigen-induced neutrophil migration during inflammation, triggering a sequential OVA, IL-15, IL-18, MIP-2, MIP-1alpha, TNF-alpha, LTB4 and neutrophil migration signaling cascade.
Feature Extraction for ASR (2011)

This chapter contains sections titled: Introduction Common Feature Vectors Dynamic Features Strategies for Robustness Auditory Models Multichannel Input Discriminant Features Discussion Exercises
Unpaired Image Captioning via Scene Graph Alignments (2019)

Most of current image captioning models heavily rely on paired image-caption datasets. However, getting large scale image-caption paired data is labor-intensive and time-consuming. In this paper, we present a scene graph-based approach for unpaired image captioning. Our framework comprises an image scene graph generator, a sentence scene graph generator, a scene graph encoder, and a sentence decoder. Specifically, we first train the scene graph encoder and the sentence decoder on the text modality. To align the scene graphs between images and sentences, we propose an unsupervised feature alignment method that maps the scene graph features from the image to the sentence modality. Experimental results show that our proposed model can generate quite promising results without using any image-caption training pairs, outperforming existing methods by a wide margin.
Large area direct-write focused ion-beam lithography with a dual-beam microscope (2010)

The authors have investigated the performance of focused ion-beam (FIB) direct-write lithography for large area (multiple write-field) patterning in an FEI Nova Nanolab 600 dual-beam microscope. Their system is configured with a 100 nm resolution X-Y stage and a RAITH ELPHY LITHOGRAPHY control interface, with its own integrated 16 bit DAC pattern generator and software. Key issues with regard to configuration, process parameters, and procedures have been addressed. Characterization of stitching errors, pattern repeatability, and drift were performed. Offset lithography (multiple exposures with offset write fields) and in-field registration marks were evaluated for correcting stitching errors, and a test microfluidic device covering an area of 1×1.4 mm2 was successfully fabricated. The authors found that by using a combination of offset lithography and in-field registration mark correction methods, the stitching errors can be kept well below 100 nm. They also found that due to higher beam deflection speed provided by the electrostatic scanning in FIB systems versus the wide-spread electron-beam systems with electromagnetic scanning, FIB lithography can be just as fast as electron-beam lithography for typical mill depths down to about 200–500 nm (material dependent). This opens the door for a large suite of applications for materials where pattern transfer is difficult or impossible by reactive methods.
Comprehensive Thermochemistry of W–H Bonding in the Metal Hydrides CpW(CO)2(IMes)H, [CpW(CO)2(IMes)H]•+, and [CpW(CO)2(IMes)(H)2]+. Influence of an N-Heterocyclic Carbene Ligand on Metal Hydride Bond Energies (2011)

The free energies interconnecting nine tungsten complexes have been determined from chemical equilibria and electrochemical data in MeCN solution (T = 22 °C). Homolytic W-H bond dissociation free energies are 59.3(3) kcal mol(-1) for CpW(CO)(2)(IMes)H and 59(1) kcal mol(-1) for the dihydride [CpW(CO)(2)(IMes)(H)(2)](+) (where IMes = 1,3-bis(2,4,6-trimethylphenyl)imidazol-2-ylidene), indicating that the bonds are the same within experimental uncertainty for the neutral hydride and the cationic dihydride. For the radical cation, [CpW(CO)(2)(IMes)H](•+), W-H bond homolysis to generate the 16-electron cation [CpW(CO)(2)(IMes)](+) is followed by MeCN uptake, with free energies for these steps being 51(1) and -16.9(5) kcal mol(-1), respectively. Based on these two steps, the free energy change for the net conversion of [CpW(CO)(2)(IMes)H](•+) to [CpW(CO)(2)(IMes)(MeCN)](+) in MeCN is 34(1) kcal mol(-1), indicating a much lower bond strength for the 17-electron radical cation of the metal hydride compared to the 18-electron hydride or dihydride. The pK(a) of CpW(CO)(2)(IMes)H in MeCN was determined to be 31.9(1), significantly higher than the 26.6 reported for the related phosphine complex, CpW(CO)(2)(PMe(3))H. This difference is attributed to the electron donor strength of IMes greatly exceeding that of PMe(3). The pK(a) values for [CpW(CO)(2)(IMes)H](•+) and [CpW(CO)(2)(IMes)(H)(2)](+) were determined to be 6.3(5) and 6.3(8), much closer to the pK(a) values reported for the PMe(3) analogues. The free energy of hydride abstraction from CpW(CO)(2)(IMes)H is 74(1) kcal mol(-1), and the resultant [CpW(CO)(2)(IMes)](+) cation is significantly stabilized by binding MeCN to form [CpW(CO)(2)(IMes)(MeCN)](+), giving an effective hydride donor ability of 57(1) kcal mol(-1) in MeCN. Electrochemical oxidation of [CpW(CO)(2)(IMes)](-) is fully reversible at all observed scan rates in cyclic voltammetry experiments (E° = -1.65 V vs Cp(2)Fe(+/0) in MeCN), whereas CpW(CO)(2)(IMes)H is reversibly oxidized (E° = -0.13(3) V) only at high scan rates (800 V s(-1)). For [CpW(CO)(2)(IMes)(MeCN)](+), high-pressure NMR experiments provide an estimate of ΔG° = 10.3(4) kcal mol(-1) for the displacement of MeCN by H(2) to give [CpW(CO)(2)(IMes)(H)(2)](+).
Design and Analysis of the Three-Dimensional Bar Code (2008)

The bar code need increase its information capacity because of its widespread use. In this paper, a novel bar code- three-dimensional bar code is proposed. It can greatly increase the information capacity. The three-dimensional bar code combines the variety of widths, the variety of colors and a vertical array to provide significantly more information content. At first, the encoding technology of bar code is discussed. Then the three-dimensional bar code is proposed on the basis of the two-dimensional bar code. The design principle and information content of the three-dimensional bar is also discussed. Finally, the experiment proves the three-dimensional bar code can withstand the influence of noise and blur.
Design of a new class of spreading sequence using chaotic dynamical systems for asynchronous DS-CDMA applications (2004)

This paper investigates a novel method to derive a set of binary sequences from a chaotic dynamical system. We modify the previous methods using statistical properties of chaotic sequences. It has been shown that the proposed sequences have good performance according to merit factor and W-index criteria and can be used as a spreading sequence in asynchronous DS-CDMA systems.
Overlooked aspects concerning development and spread of antimicrobial resistance (2003)

(2003). Overlooked aspects concerning development and spread of antimicrobial resistance. Expert Review of Anti-infective Therapy: Vol. 1, No. 3, pp. 359-362.
A method for the preparation of methaemoglobin-containing human red cell suspensions. (1992)

The variables affecting the formation and stability of intracellular methaemoglobin were examined. A method is proposed for the preparation of intracellular methaemoglobin specimens.
Degos disease complicated by constrictive pericarditis in remote phase: a case report (2022)

The study findings suggests that Degos disease can cause long-term CP. Aspirin effectively inhibited the progression of Degos disease, and surgical treatment was necessary when heart failure due to CP was refractory to treatment.
Unmanned Aerial Vehicle Systems for Remote Estimation of Flooded Areas Based on Complex Image Processing (2017)

Floods are natural disasters which cause the most economic damage at the global level. Therefore, flood monitoring and damage estimation are very important for the population, authorities and insurance companies. The paper proposes an original solution, based on a hybrid network and complex image processing, to this problem. As first novelty, a multilevel system, with two components, terrestrial and aerial, was proposed and designed by the authors as support for image acquisition from a delimited region. The terrestrial component contains a Ground Control Station, as a coordinator at distance, which communicates via the internet with more Ground Data Terminals, as a fixed nodes network for data acquisition and communication. The aerial component contains mobile nodes-fixed wing type UAVs. In order to evaluate flood damage, two tasks must be accomplished by the network: area coverage and image processing. The second novelty of the paper consists of texture analysis in a deep neural network, taking into account new criteria for feature selection and patch classification. Color and spatial information extracted from chromatic co-occurrence matrix and mass fractal dimension were used as well. Finally, the experimental results in a real mission demonstrate the validity of the proposed methodologies and the performances of the algorithms.
Infection in the Pathogenesis of Bronchopulmonary Dysplasia (1999)

Improvements in strategies for care of preterm infants with respiratory distress over the past decade have changed the major clinical conditions associated with development of chronic pulmonary disease, as reflected by a requirement for oxygen support at 28 days of age or a gestational age of 36 weeks. Two decades ago, the factors most strongly related to development of bronchopulmonary dysplasia (BPD) were prolonged exposure to high inspired oxygen concentrations and barotrauma, as indicated by high peak inspiratory and mean airway pressures, particularly in preterm infants. As antenatal induction of lung maturation by maternal steroid therapy, reduction of supplemental oxygen, and positive-pressure ventilation requirements by surfactant replacement therapy, and refinement of conventional and high-frequency ventilation strategies to minimize barotrauma have reduced the mortality and morbidity of chronic lung disease among premature infants, pulmonary infection appears to have become a more significant contributor to the pathogenesis of chronic oxygen dependency in infancy. The goal of this chapter is to review the epidemiological and pathogenetic observations that may illuminate this relation.