gebhart/patrepeval huggingface dataset:
Molecular Cloning of the Full-length cDNA Encoding Mouse Neutral Ceramidase (2000)

We report here the molecular cloning, sequencing, and expression of the gene encoding the mouse neutral ceramidase, which has been proposed to function in sphingolipid signaling. A full-length cDNA encoding the neutral ceramidase was cloned from a cDNA library of mouse liver using the partial amino acid sequences of the purified mouse liver ceramidase. The open reading frame of 2,268 nucleotides encoded a polypeptide of 756 amino acids having nine putative N-glycosylation sites. Northern blot analysis revealed that the mRNA of the ceramidase was expressed widely in mouse tissues, with especially strong signals found in the liver and kidney. The ceramidase activity of lysates of CHOP cells increased more than 900-fold when the cells were transformed with a plasmid containing the cDNA encoding ceramidase. We also cloned the ceramidase homologue from the cDNA library of mouse brain and found that the sequence of the open reading frame, but not the 5'-noncoding region, was identical to that of the liver. Interestingly, phylogenetic analysis of various ceramidases clearly indicated that neutral/alkaline ceramidases form a novel but highly conserved gene family that is evolutionarily different from lysosomal acid ceramidases.
The potential superiority of bromodeoxyuridine to iododeoxyuridine as a radiation sensitizer in the treatment of colorectal cancer. (1992)

Although the thymidine analogues 5-bromo-2'-deoxyuridine (BrdUrd) and 5-iodo-2'-deoxyuridine (IdUrd) have been used successfully as radiation sensitizers in clinical trials, it is not clear which of these agents is the more promising to pursue. To begin to assess this question with regard to colorectal cancer metastatic to the liver, a study was carried out using HT29 human colon cancer cells in culture and implanted in nude mice as xenografts. Cells and animals were treated with BrdUrd +/- the thymidylate synthase inhibitor 5-fluoro-2'-deoxyuridine (FdUrd), and the results compared to our previous studies with IdUrd +/- FdUrd (T. S. Lawrence, M. A. Davis, P. E. McKeever, J. Maybaum, P. L. Stetson, D. P. Normolle, and W. D. Ensminger. Cancer Res., 51: 3900-3905, 1991). Using cultured cells, it was found that FdUrd (at concentrations of greater than 10 nM) increased: (a) the incorporation of BrdUrd into the DNA of cultured tumor cells; (b) BrdUrd-mediated radiosensitization; (c) BrdUrd-mediated increase in radiation-induced DNA damage; and (d) BrdUrd-mediated decrease in the repair of radiation-induced damage. The incorporation of BrdUrd was greater than or equal to the incorporation of IdUrd previously determined under the same exposure conditions. Studies using nude mice bearing HT29 xenografts showed that FdUrd increased BrdUrd incorporation more into tumors than into the normal liver. Most tumor cells incorporated BrdUrd (labeling index after a 4-day infusion = 87 +/- 2%; SE); in the liver, labeling was confined chiefly to nonparenchymal cells. In both the presence and absence of FdUrd, the incorporation of BrdUrd into tumors was significantly and consistently greater than the incorporation of IdUrd measured under the same conditions of drug administration (by a factor of 1.2-3.6). Furthermore, the administration of BrdUrd +/- FdUrd tended to produce less weight loss and hematological toxicity than IdUrd +/- FdUrd. These findings suggest that BrdUrd may be superior to IdUrd as a radiation sensitizer in the treatment of colorectal cancer metastatic to the liver.
Two-round Multiparty Secure Computation from Minimal Assumptions (2022)

We provide new two-round multiparty secure computation (MPC) protocols in the dishonest majority setting assuming the minimal assumption that two-round oblivious transfer (OT) exists. If the assumed two-round OT protocol is secure against semi-honest adversaries (in the plain model) then so is our two-round MPC protocol. Similarly, if the assumed two-round OT protocol is secure against malicious adversaries (in the common random/reference string model) then so is our two-round MPC protocol. Previously, two-round MPC protocols were only known under relatively stronger computational assumptions.
Isoxazoles. XVIII. Synthesis and Pharmacological Properties of 5-Aminoalkyl- and 3-Aminoalkylisoxazoles and Related Derivatives (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsoxazoles. XVIII. Synthesis and Pharmacological Properties of 5-Aminoalkyl- and 3-Aminoalkylisoxazoles and Related DerivativesHideo. Kan[UNK]o, Ikuo. Adachi, Ryonosuke. Kido, and Katumi. HiroseCite this: J. Med. Chem. 1967, 10, 3, 411–418Publication Date (Print):May 1, 1967Publication History Published online1 May 2002Published inissue 1 May 1967https://pubs.acs.org/doi/10.1021/jm00315a028https://doi.org/10.1021/jm00315a028research-articleACS PublicationsRequest reuse permissionsArticle Views390Altmetric-Citations66LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Physiological effects of high-P50 erythrocyte transfusion on piglets (1985)

Rightward shifts of the O2 dissociation curve (ODC) were experimentally obtained in lysed and resealed erythrocytes following encapsulation of inositol hexaphosphate (IHP). This continuous lysing and resealing procedure led to in vitro P50 (Po2 at 50% hemoglobin saturation) increases up to 80 Torr (pH, 7.40; Pco2, 40 Torr; temp, 37 degrees C) for both human and pig erythrocytes. The Hill number of the transformed blood decreased when IHP was fixed on the hemoglobin, but the sigmoid shape of the ODC was maintained. The O2 hemoglobin binding capacity and the mean corpuscular hemoglobin content were found unchanged by the experimental procedure in human and pig erythrocytes. Isovolumic exchange transfusion of high-P50 erythrocytes in anesthetized and ambient air-ventilated piglets (n = 6) led to substantial in vivo P50 increases (range, 8-19 Torr). The rightward shift of the ODC was concomitant with an increase of the arterial Po2 and of the arteriovenous O2 content difference, 19 and 59% respectively above their control values. The mixed-venous Po2 (PVO2) remained unchanged. The cardiac output was shown to be inversely related to the P50 value. In spite of the O2-transport reduction (37%), O2 consumption was maintained due to enhanced O2 extraction.
Combfit: A Normalization Method for Array CGH Data (2006)

The recently developed array-based comparative genomic hybridization(array CGH) technique measures DNA copy number aberrations that occur as causes or consequences of cell diseases such as cancers. Conventional array CGH analysis classifies DNA copy number aberrations into three categories: no significant change, significant gain, and significant loss. However, recent improvements in microarray measurement precision enable more quantitative analysis of copy number aberrations. We propose a method, called comb fitting, that extracts a quantitative interpretation from array CGH data. We also propose modifications that allow us to apply comb fitting to cases featuring heterogeneity of local aberrations in DNA copy numbers. By using comb fitting, we can correct the baseline of the fluorescence ratio data measured by array CGH and simultaneously translate them into the amount of changed copy numbers for each small part of the chromosome, such as 0, ±1, ±2, ···. Comb fitting is applicable even when a considerable amount of contamination by normal cells exists and when heterogeneity in the ploidy number cannot be neglected.
Molecular engineering versus energy level alignment: Interface formation between oligothiophene derivatives and a metal substrate studied with photoemission spectroscopy (2002)

Two series of thin films of oligothiophene derivatives grown on Ag substrates have been studied with photoelectron spectroscopy. The oligothiophenes were end-capped with either electron-deficient (dismesitylboryl) or electron-rich (diphenyltolylamine) moieties to create molecules with electron-accepting or -donating properties, respectively. The position of the highest occupied molecular orbital (HOMO) at the metal/organic interface is found to be strongly dependent on the effective π-conjugation length of the oligothiophenes capped with dimesitylboryl groups, whereas in the oligothiophenes capped with diphenyltolylamine, the position of the HOMO is independent of the molecular length. The difference in the observed HOMO characteristics is attributed to the different make-up of the frontier orbitals in the two molecular series. This will particularly affect the overall energy barrier for charge injection at the conductor/organic interface in a device structure, such as an organic light-emitting diode, utilizing the investigated molecules for carrier injection and transport.
Immunity to Citrullinated Proteins in Rheumatoid Arthritis (2008)

Antibodies to citrullinated proteins (ACPA), i.e., to peptides posttranslationally modified by the conversion of arginine to citrulline, are specific serological markers for rheumatoid arthritis (RA). Studies on anticitrulline immunity, summarized in this review, demonstrate that the criterion-based syndrome RA should be subdivided into at least two distinct subsets (ACPA-positive and ACPA-negative disease). A new etiological model is proposed for ACPA-positive RA, built on MHC class II-dependent activation of adaptive immunity. Fundamentals of this model include the following: (a) ACPA antedate onset of arthritis; (b) ACPA may aggravate arthritis in rodents; (c) ACPA are triggered in the context of genes that confer susceptibility to RA (HLA-DRB1 SE) and by environmental agents triggering RA (smoking or bacterial stimuli); (d) ACPA may complex with citrullinated proteins present in target tissue as part of a multistep process for arthritis development. The model provides a new basis for molecular studies on the pathogenesis of ACPA-positive arthritis.
Transscleral Drug Delivery to the Retina and Choroid (2006)

Transscleral delivery is a nondestructive and minimally invasive method that achieves targeted delivery of high molecular weight compounds to the posterior segment. A long-term transscleral delivery device may be clinically feasible because the human eye is remarkably tolerant of foreign bodies overlying the sclera, such as scleral buckles used in treating retinal detachment, even for years. The clinically used anesthetic benzalkonium chloride was found to enhance transscleral penetration of molecules as large as 20 kDa dextran into the retina and choroid. The delivery of drug to the retina may be somewhat surprising in the face of the classical belief that the retinal pigment epithelium forms a strict barrier to drug penetration; however, a study has shown that even molecules as large as 80 kDa dextrans can diffuse through this tissue. Drug characteristics such as its ionization and molecular shape also affect the rate of transscleral diffusion.
Development and Optimization of a Rotating Phased Array Inspection System (2003)

This contribution describes the approach pursued in the development of a miniaturized phased array system. Aiming at the detection of corrosion damage at the inner and outer surface, an array transducer has first been optimized for immersion inspection of pipes with high radial and axial resolution. In a second step, system components have been designed and developed on the basis of highly integrated electronic components.
Size-Selective Nanoparticle Assembly on Substrates by DNA Density Patterning (2016)

The vision of nanoscale self-assembly research is the programmable synthesis of macroscale structures with controlled long and short-range order that exhibit a desired set of properties and functionality. However, strategies to reliably isolate and manipulate the nanoscale building blocks based on their size, shape, or chemistry are still in their infancy. Among the promising candidates, DNA-mediated self-assembly has enabled the programmable assembly of nanoparticles into complex architectures. In particular, two-dimensional assembly on substrates has potential for the development of integrated functional devices and analytical systems. Here, we combine the high-resolution patterning capabilities afforded by electron-beam lithography with the DNA-mediated assembly process to enable direct-write grayscale DNA density patterning. This method allows modulation of the functionally active DNA surface density to control the thermodynamics of interactions between nanoparticles and the substrate. We demonstrate that size-selective directed assembly of nanoparticle films from solutions containing a bimodal distribution of particles can be realized by exploiting the cooperativity of DNA binding in this system. To support this result, we study the temperature-dependence of nanoparticle assembly, analyze the DNA damage by X-ray photoelectron spectroscopy and fluorescence microscopy, and employ molecular dynamics simulations to explore the size-selection behavior.
Improvement of Solar Cells Efficiency and Radiation Stability by Deposition of Diamond-Like Carbon Films (2011)

Electricity is usually supplied by diesel generators in remote communities at costs that can reach up to $1.50 per kWh in northern Canada.At these costs, several renewable energy sources (RESs) such as wind and photovoltaic (PV) can be cost effective to meet part of the energy needs.Their main drawback, being fluctuating and intermittent, can be compensated with either storage units, which are costly, and/or by adapting the electrical power consumption (load) to the availability of RESs.Electric water heaters (EWHs) are good candidates for demand side management (DMS) because of their relatively high power ratings and intrinsic thermal energy storage capabilities.The average power consumed by an EWH is strongly related to the set point temperature (Td) and to the hot water draw (Wd).A 5.5 kW, 50 gallon EWH is modeled in MATLAB-Simulink and a typical 24-hour water draw profile is used to estimate the potential range of power variation offered by an EWH for power balancing purposes.Besides, a strategy for controlling the power consumed by the EWH, by means of Td, using a grid frequency versus temperature/power droop characteristic is proposed.In this way, the EWH can be used for power balancing and for assisting with the mini-grid frequency control.
Preface (2007)

Techniques in Orthopaedics: March 2007 - Volume 22 - Issue 1 - p 1 doi: 10.1097/01.bto.0000261727.90028.1c
Infrared frequency comb for frequency metrology based on a tunable repetition rate fiber laser (2004)

A phase-locked, all-fiber supercontinuum source based on an ultrashort fiber ring laser with a tunable repetition rate is presented. The supercontinuum output is composed of a frequency comb with a spacing set by the laser repetition rate and an offset frequency determined by the carrier-envelope offset frequency. The laser repetition rate can be scanned over 400 kHz while the offset frequency remains phase-locked to a stable RF source.
Building your vision with Qualcomm's Mobile Augmented Reality (AR) platform: AR on mobile devices (2011)

Academic/Research Overview of Mobile Augmented Reality Introduction to the Qualcomm AR Platform — Features and Usage Scenarios Developing Mobile AR Applications using Qualcomm's Unity Extension Introduction to Qualcomm's QCAR SDK Native API Cross Platform Development with the Native QCAR SDK
A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon (2016)

Reticulons (RTNs) are a class of endoplasmic reticulum (ER) membrane proteins that are capable of maintaining high membrane curvature, thus helping shape the ER membrane into tubules. The mechanism of action of RTNs is hypothesized to be a combination of wedging, resulting from the transmembrane topology of their conserved reticulon homology domain, and scaffolding, arising from the ability of RTNs to form low-mobility homo-oligomers within the membrane. We studied the plant RTN isoform RTN13, which has previously been shown to locate to ER tubules and the edges of ER cisternae and to induce constrictions in ER tubules when overexpressed, and identified a region in the C terminus containing a putative amphipathic helix (APH). Here we show that deletion of this region or disruption of the hydrophobic face of the predicted helix abolishes the ability of RTN13 to induce constrictions of ER tubules in vivo. These mutants, however, still retain their ability to interact and form low-mobility oligomers in the ER membrane. Hence, our evidence indicates that the conserved APH is a key structural feature for RTN13 function in vivo, and we propose that RTN, like other membrane morphogens, rely on APHs for their function.
A multi-platform bathyphotometer for fine-scale, coastal bioluminescence research (2005)

Although bioluminescence (BL) in the open ocean has been extensively studied, coastal BL remains poorly understood due, in large degree, to a lack of BL instrumentation appropriate to measure the fine-scale biological and physical complexity of the coastal regime. As a contribution toward understanding coastal BL, we developed the Multipurpose Bioluminescence Bathyphotometer (MBBP). This compact, self-contained bathyphotometer (BP) was designed to function in a variety of deployment modes, including conventional shipboard profilers, towed platforms, autonomous underwater vehicles (AUVs), and profiling moorings. In all configurations, the instrument preserves signal structure at centimeter to meter scale resolution, the scale at which higher-flow instruments might disturb thin layers and other fine-scale water column features. In the MBBP, seawater is conveyed with minimal premeasurement excitation into a light-baffled stimulation and measurement chamber at a continuously measured flow rate of 350 to 400 mL s−1. A photomultiplier tube (PMT) records light from bioluminescent organisms after they are mechanically stimulated at the chamber entrance by a high-velocity impeller. Calibration and test protocols were developed to determine BL stimulation efficiency and MBBP measurement characteristics. To illustrate the capabilities of the MBBP to resolve the fine-scale structure of the BL community, measurements from two coastal environments are presented.
An Automatic Detection Algorithm of Metro Passenger Boarding and Alighting Based on Deep Learning and Optical Flow (2021)

Urban rail transit is one of the important application areas of autonomous driving, which will require an algorithm to monitor the exchange of passengers between metro carriages and stations. To this end, this article first proposes a metro passenger detection (MPD) algorithm to track passengers getting on or off, namely the MPD algorithm. The algorithm is composed of two MetroNexts and an optical flow algorithm. The two MetroNexts are based on a novel multiple scales attention convolution (MSAC) block and detect metro carriages and passengers fast and accurately with small model size. The optical flow algorithm predicts the direction of moving passengers, which help the MPD algorithm filter out unrelated ones. Combining the coordinates and moving direction of passengers, the MPD algorithm can determine whether passengers are boarding or alighting. Based on various benchmark data sets and a new metro station data set, the experimental results have demonstrated that the MSAC block is effective to improve detection accuracy. Compared with the existing state-of-the-art detection networks, the two MetroNexts have achieved competitive results with good time/memory efficiency on pedestrian data sets. On the metro station data set, the MPD algorithm robustly recognizes target passengers, the detection speed of which remains competitive even on the embedded platform, thus becoming an intelligent instrument of the metro station.
MicroRNAs transported by exosomes in body fluids as mediators of intercellular communication in cancer (2014)

Cancer-cell communication is an important and complex process, achieved through a diversity of mechanisms that allows tumor cells to mold and influence their environment. In recent years, evidence has accumulated indicating that cells communicate via the release and delivery of microRNAs (miRNAs) packed into tumor-released (TR) exosomes. Understanding the role and mode of action of miRNAs from TR exosomes is of paramount importance in the field of cancer biomarker discovery and for the development of new biomedical applications for cancer therapeutics. In this review, we focus on miRNAs secreted via TR exosomes, which by acting in a paracrine or endocrine manner, facilitate a diversity of signaling mechanisms between cancer cells. We address their contribution as signaling molecules, to the establishment, maintenance, and enhancement of the tumor microenvironment and the metastatic niche in cancer. Finally, we address the potential role of these molecules as biomarkers in cancer diagnosis and prognosis and their impact as a biomedical tool in cancer therapeutics.
Neural Network Based Adaptive Control of Piezoelectric Actuator with Unknown Hysteresis (2007)

A multi-resolution wavelet analysis coupled with a neural network based approach is applied in the problem of fault diagnostics of industrial robots. The multi-resolution analysis implements discrete wavelet transforms with filters and decomposes the signal in various levels. The approximate and detailed coefficients of the decomposed signals are then used for training a feedforward neural network whose output determines the state (faulty or normal) of the robot. The neural network classifier was then implemented and monitored in a Matlab-Simulink environment using a state-flow model. Validation of the method was performed offline using experimental data obtained from an industrial robot manipulator used in the semi-conductor industry.
Best Practices in Measuring the Quality Factor of a Reverberation Chamber (2017)

Measurement of the quality factor of a reverberation chamber has been examined using numerical simulation and experimental measurements. The numerical simulations demonstrate that common frequency-domain and time-domain approaches yield the same measured quality factors, provided that the measurement bandwidths of each approach are equal and the frequency-domain measurements are accurately compensated for antenna-efficiency effects. Measurements performed using intentionally mismatched antennas eliminate the antenna loading effects and give the quality factor of the chamber itself. The experimental validation confirms that the frequency-domain and time-domain approaches yield the same measured quality factors under practical conditions when the antennas are well-to-moderately matched. However, the accuracy of the frequency-domain measurement is compromised when large antenna-efficiency compensation must be applied when using poorly matched test antennas. The narrowband time-domain approach with poorly matched antennas provides consistent and repeatable measurements of the quality factor of the chamber itself without antenna loading effects.