gebhart/patrepeval huggingface dataset:
Comparative analysis of phosphoprotein‐enriched myocyte proteomes reveals widespread alterations during differentiation (2004)

The differentiation of skeletal muscle has been associated with altered phosphorylation status of individual proteins. However, a global analysis of protein phosphorylation during myogenesis has yet to be undertaken. Here, we report the identification of over 130 putative phosphoproteins from murine C2C12 muscle cells. Cell extracts were fractionated on phosphoprotein enrichment columns and the resulting proteins were detected by two-dimensional gel electrophoresis and silver stain, and identified by liquid chromatography coupled to electrospray tandem mass spectrometry. The early differentiation of C2C12 myoblasts was found to be accompanied by changes in the phosphorylation or expression of numerous proteins including cytoskeletal, heat shock and signaling proteins, the pp32 family of nuclear phosphoproteins, several disease-associated gene products and other characterized and uncharacterized proteins.
Erratum for Orr et al., “Draft Genome Sequences of Two Unclassified Bacteria, Sphingomonas sp. Strains IBVSS1 and IBVSS2, Isolated from Environmental Samples” (2017)

Volume 5, no. 34, e00884-17, 2017, [https://doi.org/10.1128/genomeA.00884-17][1]. Page 2: Reference 5 should read as follows.

5. Warren RL, Yang C, Vandervalk BP, Behsaz B, Lagman A, Jones SJ, Birol I. 2015. LINKS: scalable, alignment-free scaffolding of draft genomes with long reads. Gigascience 4
ChemInform Abstract: Synthesis and Spectral Characterization of Dinuclear Palladium(I) Phenoxides and the Crystal Structure of Pd2(dppm)2(OCOCF3)2. (1990)

Abstract Eight examples of dinuclear Pd(I) phenoxide compounds of type (III) are synthesized from the corresponding dichloro compounds by metathesis with sodium or potassium aryloxides.
Evaluation of an Arm Support With Trunk Motion Capability (2016)

Due to progressive muscle weakness, the arm function in boys with Duchenne muscular dystrophy (DMD) reduces. An arm support can compensate for this loss of function. Existing arm supports are wheelchair bound, which restricts the ability to perform trunk movements. To evaluate the function of a body-bound arm support, a prototype (based on the Wilmington robotic exoskeleton (WREX) arm support) that allows trunk movements was built. In order to examine the effect of this device and to compare it with an existing wheelchair-bound device, three healthy subjects performed single joint movements (SJMs) and activities of daily living (ADL) with and without the devices. The range of motion (RoM) of the arm and the surface electromyography (sEMG) signal of five different arm muscles were measured. The range of motion increased when compared to the wheelchair-bound device, and the trunk motion was perceived as important to make specific movements easier and more natural, especially the more extreme movements like reaching for a far object and reaching to the top of the head. The sEMG signal was comparable to that of the wheelchair-bound device. This means that an arm support with trunk motion capability can increase the range of motion of the user, while the amount of support to the arm is equal.
Analysis of transcription factor- and ncRNA-mediated potential pathogenic gene modules in Alzheimer’s disease (2019)

Alzheimer's disease (AD) is a progressive neurodegenerative disease that ranks as the fourth most common cause of death in developed countries. In our study, genes differentially expressed between AD and healthy individuals were identified and used to construct protein-protein interaction (PPI) networks. The AD-related PPI network was used to identify functional modules, and enrichment analysis showed that they were significantly involved in "Alzheimer's disease", "apoptosis", and related pathways. We predicted non-coding RNAs and transcription factors that may regulate the functional modules. The expression of hub genes and transcription factors was validated in an independent data set. The results in this study provide several candidates for further research on mechanisms of AD pathogenesis.
On the Coexistence of eMBB and URLLC in Multi-Cell Massive MIMO (2023)

The non-orthogonal coexistence between the enhanced mobile broadband (eMBB) and the ultra-reliable low-latency communication (URLLC) in the downlink of a multi-cell massive MIMO system is rigorously analyzed in this work. We provide a unified information-theoretic framework blending an infinite-blocklength analysis of the eMBB spectral efficiency (SE) in the ergodic regime with a finite-blocklength analysis of the URLLC error probability relying on the use of mismatched decoding, and of the so-called saddlepoint approximation. Puncturing (PUNC) and superposition coding (SPC) are considered as alternative downlink coexistence strategies to deal with the inter-service interference, under the assumption of only statistical channel state information (CSI) knowledge at the users. eMBB and URLLC performances are then evaluated over different precoding techniques and power control schemes, by accounting for imperfect CSI knowledge at the base stations, pilot-based estimation overhead, pilot contamination, spatially correlated channels, the structure of the radio frame, and the characteristics of the URLLC activation pattern. Simulation results reveal that SPC is, in many operating regimes, superior to PUNC in providing higher SE for the eMBB yet achieving the target reliability for the URLLC with high probability. Moreover, PUNC might cause eMBB service outage in presence of high URLLC traffic loads. However, PUNC turns to be necessary to preserve the URLLC performance in scenarios where the multi-user interference cannot be satisfactorily alleviated.
Lotus Leaf (Nelumbo nucifera) and its Active Constituents Prevent Inflammatory Responses in Macrophages via JNK/NF-κB Signaling Pathway (2014)

Inflammation is a serious health issue worldwide that induces many diseases, such as inflammatory bowel disease (IBD), sepsis, acute pancreatitis and lung injury. Thus, there is a great deal of interest in new methods of limiting inflammation. In this study, we investigated the leaves of Nelumbo nucifera Gaertn, an aquatic perennial plant cultivated in eastern Asia and India, in anti-inflammatory pharmacological effects in the murine macrophage cell line RAW264.7. Results showed that lipopolysaccharide (LPS) increased the protein expression of inducible nitric oxide synthase (iNOS) and COX-2, as well as the mRNA expression and level of IL-6 and TNF-α, while NNE significantly reduced these effects of LPS. LPS also induced phospho-JNK protein expression. The JNK-specific inhibitor SP600125 decreased the proteins expression of phospho-JNK, iNOS, COX-2, and the mRNAs expression and levels of IL-6 and TNF-α. Further, NNE reduced the protein expression of phospho-JNK. LPS was also found to promote the translocation of NF-κB from the cytosol to the nucleus and to decrease the expression of cytosolic IκB. NNE and SP600125 treatment recovered the LPS-induced expression of NF-κB and IκB. While phospho-ERK and phospho-p38 induced by LPS, could not be reversed by NNE. To further investigate the major components of NNE in anti-inflammatory effects, we determined the quercetin and catechin in inflammatory signals. Results showed that quercetin and catechin significantly decreased the proteins expression of iNOS, COX-2 and phospho-JNK. Besides, the mRNAs and levels of IL-6 and TNF-α also decreased by quercetin and catechin treatment in LPS-induced RAW264.7 cells. These results showed that NNE and its major components quercetin and catechin exhibit anti-inflammatory activities by inhibiting the JNK- and NF-κB-regulated pathways and could therefore be an useful anti-inflammatory agent.
Repurposing Diltiazem for Its Neuroprotective Anti-dementia Role Against Intra-Cerebroventricular Streptozotocin-Induced Sporadic Alzheimer’s Disease-Type Rat Model (2023)

Alzheimer’s disease (AD) is an age-related neuropsychiatric disorder and a common cause of progressive dementia. Diltiazem (DTZ), the non-dihydropyridine benzothiazepine class of calcium channel blocker (CCB), used clinically in angina and other cardiovascular disorders have proven neurological benefits. In the present study, the neuroprotective anti-dementia effects of DTZ against intra-cerebroventricular-streptozotocin (ICV-STZ)-induced sporadic AD (SAD)-type rat model was investigated. ICV-STZ-induced cognitive impairments were measured by passive avoidance and Morris water maze tasks. Anti-oxidative enzyme status, pro-inflammatory markers, and amyloid-beta (Aβ) protein expression in rat brain tissues were measured by ELISA kits, Western blotting, and immunostaining techniques. Data revealed that ICV-STZ injection in rats significantly induced cognitive deficits and altered the levels of oxidative and pro-inflammatory markers (p &amp;amp;lt; 0.05 ~ p &amp;amp;lt; 0.001). Treatment with DTZ (10 mg/kg, 20 mg/kg, and 40 mg/kg. p.o.) daily for twenty-one days, 1 h before a single ICV-STZ (3 mg/kg) injection, significantly improved cognitive impairments, ameliorated the ICV-STZ-induced altered nitrite, pro-inflammatory cytokines (TNF-α, and IL-1β) and anti-oxidative enzyme levels (superoxide dismutase, lipid peroxidation, and glutathione). Further, DTZ restored the increased Aβ protein expression in ICV-STZ-induced brain tissue Considering the data obtained, DTZ exhibited a potential neuroprotective anti-dementia role in ICV-STZ-induced SAD-type conditions in rats and might be repurposed as a potential therapeutic agent in the treatment and management of AD and related dementia pathologies.
Single-Nucleotide Polymorphism Allele Frequencies Determined by Quantitative Kinetic Assay of Pooled DNA (2002)

The identification of genes for complex traits relies on the detection of coinheritance of chromosomal regions in related individuals or the sharing of functional or marker alleles among phenotypically similar individuals. Single-nucleotide polymorphisms (SNPs) comprise the most abundant and accessible class of sequence polymorphisms in the genome and are valuable for high-throughput genotyping (1). Genotyping individual DNA samples is expensive in time, reagents, labor, and DNA. These limitations can be overcome by applying a sample-pooling approach for allele frequency determination before genotyping (2)(3). For quantitative allelotyping using pools, equivalent amounts of DNA from multiple individuals are combined in a single-tube assay. Most sample-pooling approaches involve post-PCR processing (4)(5)(6)(7). One pooled allelotyping method uses kinetic allelespecific PCR coupled with SYBR Green I fluorescence detection (8). This method requires two separate PCR reactions for each DNA pool, each of which needs to be performed in parallel. Another pooled allelotyping method uses a bioluminometric assay coupled to a modified primer extension reaction. This method also involves multiple procedures (9). Allelotyping by hybridization to DNA chips has been described (10), but its quantitative accuracy has not been fully evaluated.

The 5′ nuclease assay (TaqMan®) uses fluorogenic allele-specific detection probes that allow PCR amplification and allele detection in a single procedure (11). To discriminate alleles, each fluorogenic probe is labeled with a different fluorescent reporter dye. The reaction is kinetically monitored in “real time” to quantify the ratio of the two alleles (12). Taking advantage of the specificity and sensitivity of kinetic sequence detection, we developed a method for estimating SNP allele frequencies in pooled DNA samples. Accuracy was evaluated using constructed pools in which allele frequencies were incrementally varied and by comparing the allele frequencies determined from pooled DNA with allele frequencies determined by individual …
Zur Kenntnis des Kohlenstoffringes. 57. Mitteilung. Über die Dipolmomente ringhomologer Cyclanone (1951)

Abstract Die Dipolmomente einer Reihe von aliphatischen Ketonen wurden gemessen. Es wird gezeigt, dass die beobachtbare Abhängigkeit der Dipolmomente von der Ringgrösse mit Hilfe einer einfachen elektrostatischen Influenztheorie nicht erklärt werden kann.
Enhanced Secretory Activity of Atropa belladonna Hairy Root Culture Over-expressing ADP-Ribosylation Factor Gene (2008)

Agrobacterium-mediated transformation of leaf tissues of Atropa belladonna with an adenosine 5'-diphosphate (ADP)-ribosylation factor gene of carrot, arf-001, was performed employing pBCR82 as an expression vector. This vector co-expresses rol gene cluster together with arf-001, and thus, the transformed host cells were obtained as hairy roots. Two cell lines of the transformed belladonna were established as the liquid cultures of hairy root tissues, and expression of arf-001 and accumulation of its product in the cells were confirmed by RT-PCR and Western blot analyses, respectively. A marked increase in extracellular protein concentrations was observed in the transformed belladonna root cultures as compared with the controls transformed with an empty vector. However, the secretion of the proteins of the transformants was markedly reduced in the presence of a physiological concentration of monensin. These results suggest that over-expression of arf-001 in belladonna results in the enhancement of secretory activity in the transformed cells.
Optoelektronisches 3D-Positionsmesssystem (Optical 3D-Position Measuring System) (2004)

Abstract Die Positionserfassung bewegter Komponenten in der Robotik und Medizintechnik wird immer komplexer und erfordert in vielen Anwendungen die gleichzeitige Erfassung vieler Freiheitsgrade eines Objektes im Raum. Das führt zur Entwicklung von 3D-Sensoren, die die Raumlage der Roboter bzw. Instrumente bestimmen können. Ein optoelektronisches und berührungslos arbeitendes Messsystem zur dreidimensionalen Positionserfassung, basierend auf einem CCD-Flächenbildsensor, wurde entwickelt. Es kann sechs Freiheitsgrade erfassen, die drei translatorischen in x-, y- und z -Richtung und die drei rotatorischen um die jeweilige Koordinatenachse. Die experimentell bestimmten Auflösungen des Systems liegen für die x- und y-Richtung bei ca. 1 mm, für die z-Richtung bei 6 mm und für die Rotationen bei ca. 1º.
Serum Alanine Aminotransferase of Donors in Relation to the Risk of Non-A,Non-B Hepatitis in Recipients (1981)

To evaluate the incidence of post-transfusion hepatitis and factors influencing its occurrence, the Transfusion-Transmitted Viruses Study prospectively followed 1513 transfusion recipients from 1974 through 1979. The attack rate for non-A,non-B hepatitis was 10 per cent. The incidence of hepatitis was directly related to the alanine aminotransferase (ALT) level in blood donors. In recipients of multiple transfusions of blood that had no donor-ALT level above 29 IU per liter the attack rate was 6 per cent or less; at higher donor-ALT levels the attack rate increased progressively, reaching 45 per cent in recipients of units with an ALT of 60 IU or greater. A similar relation was observed among recipients of single units of blood. Moreover, hepatitis developed in 10 of 11 recipients of two units with an ALT level of 45 IU or greater. These data indicate that screening blood for ALT levels would reduce the incidence of non-A,non-B post-transfusion hepatitis.
Point of View (1993)

Total Quality Management and Continuous Quality Improvement have become the "buzz" phrase for the 1990s. Everyone seems to be jumping on the band wagon. But what is it, and is it right for your organization? We believe that Total Quality Management is superior to many Quality Assurance programs currently in use. There are, however, many considerations to take into account before implementing a Total Quality Management program. The purpose of this article is to explain the pros and cons of Total Quality Management and standard Quality Assurance programs and to compare and contrast these two approaches. This article focuses on barriers to successful implementation and presents several questions organizations can utilize in assessing readiness for a quality management program.
Urochordate Histoincompatible Interactions Activate Vertebrate-Like Coagulation System Components (2008)

The colonial ascidian Botryllus schlosseri expresses a unique allorecognition system. When two histoincompatible Botryllus colonies come into direct contact, they develop an inflammatory-like rejection response. A surprising high number of vertebrates' coagulation genes and coagulation-related domains were disclosed in a cDNA library of differentially expressed sequence tags (ESTs), prepared for this allorejection process. Serine proteases, especially from the trypsin family, were highly represented among Botryllus library ortholgues and its "molecular function" gene ontology analysis. These, together with the built-up clot-like lesions in the interaction area, led us to further test whether a vertebrate-like clotting system participates in Botryllus innate immunity. Three morphologically distinct clot types (points of rejection; POR) were followed. We demonstrated the specific expression of nine coagulation orthologue transcripts in Botryllus rejection processes and effects of the anti-coagulant heparin on POR formation and heartbeats. In situ hybridization of fibrinogen and von Willebrand factor orthologues elucidated enhanced expression patterns specific to histoincompatible reactions as well as common expressions not augmented by innate immunity. Immunohistochemistry for fibrinogen revealed, in naïve and immune challenged colonies alike, specific antibody binding to a small population of Botryllus compartment cells. Altogether, molecular, physiological and morphological outcomes suggest the involvement of vertebrates-like coagulation elements in urochordate immunity, not assigned with vasculature injury.
Lambda phage-based vaccine induces antitumor immunity in hepatocellular carcinoma (2017)

These observations suggest that ASPH may serve as a highly antigenic target for immunotherapy.
The use of dried blood spot sampling for the measurement of HbA1c: a cross-sectional study (2015)

Australian New Zealand Clinical Trials Registry ID ACTRN12613000769785.
New trends in nanobiotechnology (2023)

The widespread use of nanotechnology has reached almost every sector in our daily lives and amazed the world by offering various potential applications in these sectors. The uprising wave of nanotechnology and its application are now prominent in the fields of chemistry and biomedicine, which are vital as these fields serve as a basis for the discovery of new molecules that may benefit humans. Nanotechnology contributed to the advancement of promising techniques either by the implementation of existing methods or by the establishment of new ones. Researchers in academia and industry sectors working in areas of biochemistry, chemical engineering, molecular biology, and genetics are likely to come across the advantages of applying nanobiotechnology tools in their studies. This profound technological advantage has brought many research laboratories to globally exchange ideas and promote intensive international scientific collaborations to further increase the level of understanding of applying nanotechnology to biological systems.
Numerical analysis of extensional flow through the pharyngeal duct (2017)

The flow through the pharynx from the glossopalatal junction (GPJ) to the upper esophageal sphincter (UES) has been numerically investigated with a non-Newtonian fluid obeying the power-law with similar rheological indices to a contrast medium used in videofluroscopy. For that purpose, a three-dimensional model of the transport of food bolus along the pharynx has been proposed using the immersed boundaries method, which allow representing the shape of the pharynx using Cartesian grids. The pharyngeal wall has been considered to be an elastic membrane. Flow fields in terms of the axial velocity, pressure, shear rate and strain rate were obtained. Results show that the highest velocity concentrates in the central stream as the fluid enters into the pharynx. In addition, as the flow quits the pharynx, a recirculation zone appears inside the cavity, resulting in low velocity zone, which increases with the coefficient of elasticity. A strong dependence on the coefficient of elasticity was observed on the pressure fields; so that as such a coefficient increases, the pressure in the pharyngeal wall will increase. It has been also observed that the bolus head travels faster than the bolus tail, which indicates that the bolus is not only subjected to shear but also to elongation. Results from this work can be further used for a rheological characterization (shear and extension) of oral nutritional supplements for patients suffering from swallowing disorders.
Synthesis of Cryptophycin 52 Using the Shi Epoxidation (2002)

[reaction: see text] A synthesis of cryptophycin 52 is reported using a Shi epoxidation strategy to install the epoxide moiety in a diastereoselective fashion. Several epoxidation results for cryptophycin substrates are disclosed followed by a discussion of the details relating to the preparation of cryptophycin 52 in two synthetic steps from one of the intermediate epoxides.
Metabolism and Effects of Epidermal Growth Factor and Related Growth Factors in Mammals* (1990)

The initial observations of Stanley Cohen in the 1960s established that EGF induced in vivo effects such as precocious eyelid opening and tooth eruption. Subsequently the actions of EGF have been extensively explored in cell culture systems. The receptor for EGF was characterized as a prototype model for other growth factors and the now extensive in vitro data indicate multiple functions for EGF. Moreover, EGF and EGF receptors have been characterized in many tissues, and EGF has been identified in most body fluids of several mammalian species. Interestingly, neither EGF antibody administration to newborn animals nor passive immunization of pregnant rodents against EGF has caused major deleterious effects (except the delay in epidermal maturation events), as might be expected from the in vitro studies. This is in contrast to the effects of nerve growth factor antiserum in developing rodents. Also, to date, no pathological EGF deficiency disorder has been characterized. However, the EGF family of growth factors appears to be important in mammalian development and function, although the precise roles and significance are not yet clear. Members of the family may have a role in embryogenesis and fetal growth since receptors have been identified in fetal tissues. Available evidence suggests that TGF alpha subserves the growth factor family roles in fetal development. In the developing postnatal animal pro-EGF mRNA, immunoreactive EGF, immunoreactive TGF alpha, and EGF receptors are present in many tissues. EGF also is produced and secreted by the maternal mammary gland, and mammary derived EGF appears to be important in gut development in the neonatal rodent. There is now extensive data to indicate important hormone-EGF interactions. In the postnatal period, thyroid and steroid hormones including retinoic acid have been shown to modulate EGF and/or EGF receptors in several tissues. GH increases EGF binding in liver and increases urine EGF concentrations. Moreover, EGF stimulates secretion of several hypothalamic and pituitary hormones, increases placental production of hCG and human chorionic somatomammotropin, increases adrenal cortisol production, and inhibits testicular, ovarian, and thyroid hormone secretions. As summarized in this review EGF has been implicated in a number of developmental events including palate and skin differentiation, growth of hair follicles, eye opening and tooth eruption, lung maturation, gut and liver growth, and differentiation of neurons. These EGF actions probably are mediated via autocrine, paracrine, and endocrine routes. A role for salivary and urine EGF in the maintenance of adult stomal, gut, and urinary epithelial surface integrity seems likely, although not yet proven.(ABSTRACT TRUNCATED AT 400 WORDS)