gebhart/patrepeval huggingface dataset:
A novel method for the immobilisation and culture of plant cells (1983)

A novel technique is described for the immobilisation of plant cells. The method is simple and does not require the use of potentially toxic gels for the entrapment process. Liquid‐suspended cells of two species, Capsicum frutescens Mill. and Daucus carota L. were found to invade, and were strongly retained in, polyurethane foam particles over a 21 day period. The viability of te immobilised cells was high (70–80% were alive after 21 days). On agitation of the loaded foam particles in fresh liquid medium, at least 95% of the cells remained immobilised after 3 or 4 days.
A Methanol Sensor for Portable Direct Methanol Fuel Cells (1998)

An aqueous methanol sensor for portable direct methanol fuel cell applications is demonstrated. The design is based on current output limited by methanol diffusion through a Nafion 117 perfluorosulfonic acid membrane. Steady‐state polarization measurements demonstrate sensitivity to concentrations of 0 to 4 M over a temperature range of 40 to 80°C. Furthermore, a correlation that is first order in concentration and temperature is demonstrated for concentrations of 0 to 3 M, with an accuracy of ±0.1 M. Measurements of transient response to step concentration change indicate a response time of about 10 to 50 s, depending primarily on temperature.
Deep brain stimulation for essential tremor (1996)

We examined the effects and safety of deep brain stimulation (DBS) as a treatment for essential tremor (ET). Ten ET patients with disabling medication-refractory tremor underwent stereotactic implantation of a DBS lead in the left Vim thalamic nucleus and completed a 6-month follow-up. The Clinical Tremor Rating Scale and disability assessments were performed at baseline, 1-, 3-, and 6-month follow-up. There were significant improvements in dressing, drinking, eating, bathing, and handwriting as reported by the subjects. Tremor severity, writing, pouring, and spiral and line drawing were significantly improved as rated by the examiner. Improvements persisted through the 6-month follow-up period. Although global disability significantly lessened in the group as a whole, one subject with hand-finger tremor accentuated by writing had no change in disability status. In this 6-month open-label study, DBS was effective and safe in reducing tremor and functional disability in ET.
INFLUENCE DES TRAITEMENTS MÉCANIQUES ET HYDROTHERMIQUES SUR LA VALEUR NUTRITIVE DU MAÏS POUR LES JEUNES BOVINS A L'ENGRAIS (1972)

Quatre expriences d'engraissement de jeunes bovins ont t effectues pour tudier l'in- fluence de trois traitements technologiques sur la valeur nutritive du mas : broyage fin suivi d'un pressage (exprience i), cuisson l'eau bouillante pendant 30 ou 4 g mn (expriences 2 et 3 ) et trempage pendant 4 8 heures suivi d'un schage sur rouleaux 70 0 C (exprience 4 ). Dans chaque cas, le lot exprimental recevant le mas trait a t compar un lot tmoin recevant un mas grossirement moulu.
Homage to Chris Dobson (2019)

Homage to Chris Dobson With the purpose of remembering Chris, we collected messages from present and former collaborators. The image of Chris that comes out in this way is more choral and vibrant than what could come from more thorough but more formal obituaries. We apologize for the many colleagues who were not included because of time and space constraints.
Enhanced Read Resolution in Reconfigurable Memristive Synapses for Spiking Neural Networks (2023)

Synapse is a key element of any neuromorphic computing system which is mostly constructed with memristor devices. A memristor is a two-terminal analog memory device. Memristive synapse suffers from various challenges such as forming at high voltage, SET, RESET failure, and READ margin or resolution issue between two weights. Enhanced READ resolution is very important to make a memristive synapse functionally reliable. Usually, the READ resolution is very small for a memristive synapse with 4-bit data precision. This work considers a step-by-step analysis to enhance the READ current resolution for a current-controlled memristor-based synapse. An empirical model is used to characterize the HfO2-based memristive device. 1st and 2nd stage device of our proposed synapse can be scaled to enhance the READ current margin up to ~ 4.3x and ~ 21% respectively. Moreover, READ current resolution can be enhanced with run-time adaptation features such as READ voltage scaling and body biasing. The READ voltage scaling and body biasing can improve the READ current resolution by about 46% and 15% respectively. TENNLabs' neuromorphic computing framework is leveraged to evaluate the effect of READ current resolution on classification applications. Higher READ current resolution shows better accuracy than lower resolution with different percentages of read noise scenarios.
Numerical Analysis and Experimental Estimation of a Low-Leakage Injection Technique for Capillary Electrophoresis (2003)

This paper presents an experimental and numerical investigation into the use of low-leakage injection techniques to deliver sample plugs within electrophoresis microchips. The study addresses the principal material transport mechanisms such as electrokinetic migration, fluid flow, and diffusion and gives detail analyses to the double-L injection technique, which employs electrokinetic manipulations to avoid sample leakage within the microchip. Electrical potential contour, velocity vector, and streamline distribution in the micro CE chip are successfully developed. Experimental and numerical testing results show the double-L injection technique is capable of reducing sample leakage within cross-form microfluidic chips. The current study confirms the double-L injection technique has an exciting potential for use in high-quality, high-throughput chemical analysis applications and in many other applications throughout the field of micro total analysis systems.
Ce-doped SiO 2 optical fibers for remote radiation sensing and measurement (2009)

Scintillating materials, able to convert energy of ionizing radiation into light in the visible-UV interval, are presently used in a wide class of applications such as medical imaging, industrial inspection, security controls and high energy physics detectors. In the last few years we studied and developed a new radiation sensor based on silica-glass fiber-optic technology. In its simplest configuration such device is composed by a short portion (about 10 mm) of scintillating fiber coupled to a photomultiplier through a suitably long passive silica fiber. In this work, we present new results concerning the characterization of silica based Ce and Eu doped fibers glasses obtained by a modified sol-gel method and drawn by a conventional drawing tower for optical fibers. The radio-luminescence of Eu doped fibers is rather weak; moreover it displays a marked sensitivity increase during subsequent irradiations, preventing the use of such fibers in dosimetry. On the other hand Ce-doped fibers show very high radiation hardness, signal stability and reproducibility, and high sensitivity to radiations with energies from 10 keV to several tens of MeV. Numerous tests with photons (X and gamma rays), electrons, and protons have already been successfully performed. At the early stage of its market introduction it is the smallest radiation sensor, also compared to MOSFET and diode technology and it appears to be the ideal choice for in vivo measurements in medical field or remote sensing.
Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics (2016)

Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet the range of useful and well-characterized fluorescent proteins (XFPs) is limited. We generated and characterized three recoded fluorescent proteins for 3-color analysis in S.pombe, Super-folder GFP, monomeric Kusabira Orange 2 and E2Crimson. Upon optimization and expression in S. pombe, the three proteins enabled sensitive simultaneous 3-color detection capability. Furthermore, we describe a strategy that combines a pulse-chase approach and mathematical modeling to quantify the maturation kinetics of these proteins in vivo. We observed maturation kinetics in S. pombe that are expected from those described for these proteins in vitro and/or in other cell types, but also unpredicted behaviors. Our studies provide a kinetically-characterized, integrated three-color XFP toolbox for S. pombe.
Hydrogen Reactions of Nodulated Leguminous Plants (1978)

The interaction between the ATP-dependent evolution of H(2) catalyzed by nitrogenase and the oxidation of H(2) via a hydrogenase has been postulated to influence the efficiency of the N(2)-fixing process in nodulated legumes. A comparative study using soybean (Glycine max L. Merr.) cv. Anoka inoculated with either Rhizobium japonicum strain USDA 31 or USDA 110 and cowpea (Vigna unguiculata L. Walp.) cv. Whippoorwill inoculated with Rhizobium strain 176A27 or 176A28 cultured on a N-free medium was conducted to address this question. Nodules from the Anoka cultivar inoculated with USDA 31 evolved H(2) in air and the H(2) produced accounted for about 30% of the energy transferred to the nitrogenase system during the period of active N(2) fixation. In contrast the same soybean cultivar inoculated with USDA 110 produced nodules with an active hydrogenase and consequently did not evolve H(2) in air. A comparison of Anoka soybeans inoculated with the two different strains of R. japonicum showed that mean rates of C(2)H(2) reduction and O(2) consumption and mean mass of nodules taken at four times during vegetative growth were not significantly different.When compared to Anoka inoculated with USDA 31, the same cultivar inoculated with USDA 110 showed increases in total dry matter, per cent nitrogen, and total N(2) fixed of 24, 7, and 31%, respectively. Cowpeas in symbiosis with the hydrogenase-producing strain 176A28 in comparison with the same cultivar inoculated with the H(2)-evolving strain 176A27 produced increases in plant dry weight and total N(2) fixed of 11 and 15%, respectively. This apparent increase in the efficiency of N(2) fixation for nodulated legumes capable of reutilizing the H(2) evolved from nitrogenase is considered and it is concluded that provision of conclusive evidence of the role of the H(2)-recycling process in N(2)-fixing efficiency of legumes will require comparison of Rhizobium strains that are genetically identical with the exception of the presence of hydrogenase.
First Report of Tomato spotted wilt virus in Lettuce Crops in Saudi Arabia (2014)

A survey for viruses in open field lettuce crops was carried out in March 2014 in the Al-Uyaynah area, central region of Saudi Arabia. In one plot, more than 50% of the lettuce plants (Lactuca sativa; hybrid: Romaine), with the majority of the affected plants in the edges of the plot, were showing virus-like symptoms such as necrotic lesions, necrosis of the lamina of the younger leaves, and leaf curling, indicating a possible infection by a Tospovirus, possibly Tomato spotted wilt virus (TSWV). Most of them were dead when the field was visited again 3 weeks later. Samples from 10 symptomatic and two asymptomatic plants were collected. Five of the samples from symptomatic and two from asymptomatic plants were mechanically inoculated onto Nicotiana benthamiana and N. glutinosa (three indicator plants of each species were used for each sample) using 0.1 M phosphate buffer (pH 7) containing 0.01M Na<sub>2</sub>SO<sub>3</sub> mM. All the symptomatic lettuce samples were also tested serologically using polyclonal antisera (3) against TSWV, CMV, and by using monoclonal antibodies against potyviruses. Moreover, total RNA was extracted (1) and detection of TSWV was also attempted with reverse transcription (RT)-PCR using species specific primers (4) for a 276-bp fragment of the L RNA segment. In both serological and molecular methods, positive and negative controls were included. All the mechanically inoculated plants with tissue from the symptomatic lettuce plants of N. benthamiana showed chlorotic local lesions followed by systemic top necrosis 2 to 3 weeks post inoculation. Similarly, all inoculated N. glutinosa plants showed necrotic local lesions followed by systemic chlorosis. However, all the indicator plants mechanically inoculated with tissue from asymptomatic lettuce plants gave no reaction. All the symptomatic lettuce samples reacted positively, while asymptomatic samples reacted negatively in ELISA tests with TSWV antiserum and the presence of the virus was further confirmed by RT-PCR by using specific primers (method A) (4). PCR products of two randomly selected positive samples were directly sequenced and BLAST analysis of the obtained sequences (Accession Nos. KJ701035 and KJ701036) revealed 99% nucleotide and 100% amino acid identity with the deposit sequence in NCBI from South Korea (KC261947). Regarding mechanical inoculation, 10 days post-inoculation, both indicator plants showed typical symptoms of TSWV infection, such as necrotic local lesions, systemic necrotic patterns, and leaf deformation. None of the symptomatic plants was found to be infected with either CMV or potyvirus. To our knowledge, this is the first report of TSWV naturally infecting lettuce in Saudi Arabia; therefore, insect vector and weed management are necessary measures to control the virus spread to other crops such as tomato and pepper (2). References: (1) E. Chatzinasiou et al. J. Virol. Meth. 169:305, 2010. (2) E. K. Chatzivassiliou. Plant Dis. 92:1012, 2008. (3) E. K. Chatzivassiliou et al. Phytoparasitica 28:257, 2000. (4) R. A. Mumford et al. J. Virol. Meth. 46:303, 1994.
China's Energy Economy: Technical Change, Factor Demand and Interfactor/Interfuel Substitution (2009)

With its rapid economic growth, China's primary energy consumption has exceeded domestic energy production since 1994, leading to a substantial expansion in energy imports, particularly of oil. China's energy demand has an increasingly significant impact on global energy markets. In this paper Allen partial elasticities of factor and energy substitution, and price elasticities of energy demand, are calculated for China using a two-stage translog cost function approach. The results suggest that energy is substitutable with both capital and labour. Coal is significantly substitutable with electricity and complementary with diesel while gasoline and electricity are substitutable with diesel. China's energy intensity is increasing during the study period (1995-2004) and the major driver appears to be due to the increased use of energy intensive technology.
A Patient Presenting with Diffuse Cutaneous Leishmaniasis (DCL) as a First Indicator of HIV Infection in India (2011)

Opportunistic parasitic infections such as leishmaniasis are common in human immunodeficiency virus (HIV)-infected patients and are usually acquired several days after initial diagnosis of HIV infection. Here, we report on a patient who presented with diffuse cutaneous leishmaniasis (DCL) caused by Leishmania tropica as the first and only clinical manifestation of HIV infection. To the best of our knowledge, this is the first case that illustrates that DCL could be the first clinical indicator of HIV infection. Cutaneous leishmaniasis (CL) and DCL are becoming frequent opportunistic infections in HIV-infected individuals throughout the world. To date, all documented cases of CL and HIV coinfections have been reported in patients who were known cases of HIV and who subsequently developed CL. In this report, we present a case that illustrates that DCL could be the first clinical indicator of HIV infection.
Q-WordNet: Extracting Polarity from WordNet Senses (2010)

This paper presents Q-WordNet, a lexical resource consisting of WordNet senses automatically annotated by positive and negative polarity. Polarity classification amounts to decide whether a text (sense, sentence, etc.) may be associated to positive or negative connotations. Polarity classification is becoming important for applications such as Opinion Mining and Sentiment Analysis, which facilitates the extraction and analysis of opinions about commercial products, on companies reputation management, brand monitoring, or to track attitudes by mining online forums, blogs, etc. Inspired by work on classification of word senses by polarity (e.g., SentiWordNet), and taking WordNet as a starting point, we build Q-WordNet. Instead of applying external tools such as supervised classifiers to annotated WordNet synsets by polarity, we try to effectively maximize the linguistic information contained in WordNet, thereby taking advantage of the human effort put by lexicographers and annotators. The resulting resource is a subset of WordNet senses classified as positive or negative. In this approach, neutral polarity is seen as the absence of positive or negative polarity. The evaluation of Q-WordNet shows an improvement with respect to previous approaches. We believe that Q-WordNet can be used as a starting point for data-driven approaches in sentiment analysis. 1.
Atomic resolution map of the soluble amyloid beta assembly toxic surfaces (2019)

Soluble amyloid beta assemblies (Aβ <sub><i>n</i></sub> ) are neurotoxic and play a central role in the early phases of the pathogenesis cascade leading to Alzheimer's disease. However, the current knowledge about the molecular determinants of Aβ <sub><i>n</i></sub> toxicity is at best scant. Here, we comparatively analyze Aβ <sub><i>n</i></sub> prepared in the absence or presence of a catechin library that modulates cellular toxicity. By combining solution NMR with dynamic light scattering, fluorescence spectroscopy, electron microscopy, wide-angle X-ray diffraction and cell viability assays, we identify a cluster of unique molecular signatures that distinguish toxic <i>vs.</i> nontoxic Aβ assemblies. These include the exposure of a hydrophobic surface spanning residues 17-28 and the concurrent shielding of the highly charged N-terminus. We show that the combination of these two dichotomous structural transitions promotes the colocalization and insertion of β-sheet rich Aβ <sub><i>n</i></sub> into the membrane, compromising membrane integrity. These previously elusive toxic surfaces mapped here provide an unprecedented foundation to establish structure-toxicity relationships of Aβ assemblies.
Impact of pretransplant serum ferritin level on risk of invasive mold infection after allogeneic hematopoietic stem cell transplantation (2014)

Invasive mold infections (IMI) are life-threatening complications of allogeneic hematopoietic stem cell transplantation (HSCT) and are mostly caused by Aspergillus species and Mucorales. We examined whether elevated serum ferritin prior to HSCT was associated with increased risk of IMI after allogeneic HSCT. Elevated serum ferritin was defined as values ≥ 1000 ng/mL. Pretransplant ferritin levels were available for 477 transplants. Nine developed IMI at day 30 and 21 had IMI at day 100 for a cumulative incidence of 1.9% and 4.4%, respectively. Among the high ferritin group, eight of 220 transplant cases (3.6%) developed an IMI within 30 d after HSCT compared with one of 257 (0.4%) in the low ferritin group (P = 0.01). Fourteen of 220 (6.4%) and seven of 257 transplant cases (2.7%) in the high and low ferritin groups, respectively, had developed an IMI by day 100 after HSCT (P = 0.07). Nine of 53 (17%) patients with grades III and IV acute GVHD and iron overload experienced IMI, when compared to three of 37 (8.1%) with high-grade aGVHD, but no iron overload. Among patients without aGVHD, those with elevated ferritin had a 2.7% incidence of IMI compared with 0.9% for patients without elevated ferritin. There was a marginally significant difference in cumulative incidence function between high and low ferritin groups for IMI (P = 0.06). However, elevated serum ferritin (≥ 1000 ng/mL) was not a significant risk factor for IMI in a multivariate competing risk regression model after adjusting for aGVHD.
Improved significance test for DNA microarray data: temporal effects of shear stress on endothelial genes (2002)

Statistical methods for identifying differentially expressed genes from microarray data are evolving. We developed a test for the statistical significance of differential expression as a function of time. When applied to microarray data obtained from endothelial cells exposed to shearing for different durations, the new multi-group test (G-test) identified three times as many genes as the one-way ANOVA at the same significance level. Using simulated data, we showed that this increase in sensitivity was achieved without sacrificing specificity. Several genes known to respond to shear stress by Northern blotting were identified by the G-test at P < or = 0.01 (but not by ANOVA), with similar temporal patterns. The validity and utility of the G-test were further supported by the examination of a few more example genes in relation to the present knowledge of their regulatory mechanisms. This new significance test may have broad application for the analysis of gene-expression studies and, in fact, to other biological studies in general.
Transcription initiated by RNA polymerase II and purified transcription factors from liver. A complex set of promoter sequences governs formation of the initial complex. (1990)

Using a transcription system derived from rat liver, we have investigated the function of the TATA-box and cap site/initiator regions in assembly of stable intermediates on the pathway leading to formation of a functional RNA polymerase II preinitiation complex. Our findings argue that neither the TATA-box region nor the cap site/initiator region functions independently in the binding of transcription factors during formation of these intermediates, but, rather, the two sequence elements function cooperatively to direct assembly of a stable preinitiation complex at the promoter.
Linear and Convergent Syntheses of Bifunctional Hydroxy-Bisphosphonic Compounds as Potential Bone-Targeting Prodrugs (2019)

The synthesis of two bifunctional compounds bearing a terminal hydroxy-bisphosphonic function (HBP) was achieved following a linear and a convergent strategy. In the linear approach, the free hydroxy-bisphosphonic function was introduced in the last step of the synthesis, under neutral conditions using an Arbuzov reaction with tris(trimethylsilyl) phosphite and a carboxylic acid precursor activated in situ with catecholborane. In the convergent approach, Huisgen type cycloaddition was studied starting from an HBP-functionalized alkyne partner to obtain the targeted bifunctional molecule. These complementary approaches allow for the preparation of complex bone-targeting molecules as potential prodrug candidates.
Immunomagnetic Isolation of Mouse Embryonic Stem Cells from Heterogeneous Cell Population. (1998)

A simple immunomagnetic cell sorting method is described for the isolation and the purification of mouse embryonic stem (ES) cells from a heterogeneous cell mixture containing both ES cells and mouse embryonic fibroblast cells (MEF). The cells (1.5 × 106 cells per experiment), which labelled with the monoclonal SSEA-1 (stage specific embryonic antigen 1) antibody followed by the microbead coated with anti mouse IgM antibody, were retained in the separation column held in a magnetic field. Over 95% of the cells collected were viable after immunomagnetic cell sorting. Recoveries of 68, 82 and 90% and purity 83, 82 and 73% of the ES cells were obtained using 10, 20 and 50 μg of SSEA-1 antibody per experiment, respectively. Procedure for isolation did not affect the proliferation of the isolated cells on MEF in culture. These results suggest that the immunomagnetic cell sorting using undifferentiated cell marker antibody could be highly effective for isolating the undifferentiated cells such as ES cells.