gebhart/patrepeval huggingface dataset:
Evidence That Sprouty 2 Is Necessary for Sarcoma Formation by H-Ras Oncogene-transformed Human Fibroblasts (2007)

Sprouty 2 (Spry2) acts as an inhibitor of receptor tyrosine kinase signaling in various cellular contexts. Interestingly, Spry2 also prevents the c-Cbl-induced degradation of epidermal growth factor receptor (EGFR). We compared human fibroblasts malignantly transformed by overexpression of H-Ras(V12) oncogene to their nontransformed parental cells and found that the malignant cells express a high level of Spry2. These cells also exhibited an increase in the level of EGFR compared with their precursor cells. We found that intact EGFR was required if H-Ras-transformed cells were to grow in the absence of exogenous growth factors or form large colonies in agarose. When we decreased expression of Spry2, using a Spry2-specific shRNA, the H-Ras(V12)-transformed fibroblasts could no longer form large colonies in agarose, grow in reduced levels of serum, or form tumors in athymic mice. The level of active H-Ras in these cells remained unaltered. A similar, but less pronounced, effect in tumor formation was observed when Spry2 was down-regulated in human patient-derived fibrosarcoma cell lines. In H-Ras-transformed cells Spry2 sustained the level and the downstream signaling activity of EGFR. In the parental, non-H-Ras-transformed fibroblasts, expression of Spry2 resulted in the inhibition of H-Ras and ERK activation, suggesting that the positive effect of Spry2 in tumor formation is specific to H-Ras transformation. Co-immunoprecipitation studies with H-Ras-transformed cells revealed that Spry2 and H-Ras interact and that H-Ras interacts with Spry2-binding partners, c-Cbl and CIN85, in a Spry2-dependent manner. These data show that Spry2 plays a critical role in the ability of H-Ras-transformed cells to form tumors in athymic mice.
Effects of Tamoxifen on L-Glutamate Transporters of Astrocytes (2008)

Tamoxifen (Tam) decreased the clearance of L-glutamate (L-Glu) by cultured astrocytes at 1 pM, 1 nM, and 1 microM, but became toxic at 10 microM. When L-Glu transporters were mostly inhibited by threo-beta-benzyloxyaspartate (TBOA) (1 mM) or D,L-threo-beta-hydroxyaspartate (THA) (1 mM), Tam (1 nM) did not change extracellular L-Glu concentration, confirming that Tam attenuates L-Glu transport through L-Glu transporters. ICI182,780, LY294002, and U0126 inhibited the effect of Tam dose-dependently, suggesting the involvement of estrogen receptors (ERs), the phosphatidylinositol 3-kinase (PI3K) cascade, and the mitogen-activated protein kinase (MAPK) cascade in the effect of Tam.
Reservoir Simulation: Past, Present, and Future (1997)

Abstract Reservoir simulation is a mature technology, and nearly all major reservoir development decisions are based in some way on simulation results. Despite this maturity, the technology is changing rapidly. It is important for both providers and users of reservoir simulation software to understand where this change is leading. This paper takes a long-term view of reservoir simulation, describing where it has been and where it is now. It closes with a prediction of what the reservoir simulation state of the art will be in 2007 and speculation regarding certain aspects of simulation in 2017. Introduction Today, input from reservoir simulation is used in nearly all major reservoir development decisions. This has come about in part through technology improvements that make it easier to simulate reservoirs on one hand and possible to simulate them more realistically on the other; however, although reservoir simulation has come a long way from its beginnings in the 1950's, substantial further improvement is needed, and this is stimulating continual change in how simulation is performed. Given that this change is occurring, both developers and users of simulation have an interest in understanding where it is leading. Obviously, developers of new simulation capabilities need this understanding in order to keep their products relevant and competitive. However, people that use simulation also need this understanding; how else can they be confident that the organizations that provide their simulators are keeping up with advancing technology and moving in the right direction? In order to understand where we are going, it is helpful to know where we have been. Thus, this paper begins with a discussion of historical developments in reservoir simulation. Then it briefly describes the current state of the art in terms of how simulation is performed today. Finally, it closes with some general predictions.
Facile Synthesis of Ultrastable Fluorescent Copper Nanoclusters and Their Cellular Imaging Application (2020)

Copper nanoclusters (Cu NCs) are generally formed by several to dozens of atoms. Because of wide range of raw materials and cheap prices, Cu NCs have attracted scientists' special attention. However, Cu NCs tend to undergo oxidation easily. Thus, there is a dire need to develop a synthetic protocol for preparing fluorescent Cu NCs with high QY and better stability. Herein, we report a one-step method for preparing stable blue-green fluorescent copper nanoclusters using glutathione (GSH) as both a reducing agent and a stabilizing agent. High-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and electrospray ionization mass spectrometer (ESI-MS) were used to characterize the resulting Cu NCs. The as-prepared Cu NCs@GSH possess an ultrasmall size (2.3 ± 0.4 nm), blue-green fluorescence with decent quantum yield (6.2%) and good stability. MTT results clearly suggest that the Cu NCs@GSH are biocompatible. After incubated with EB-labeled HEK293T cells, the Cu NCs mainly accumulated in nuclei of the cells, suggesting that the as-prepared Cu NCs could potentially be used as the fluorescent probe for applications in cellular imaging.
Subthalamic DBS replaces levodopa in Parkinson’s disease (2002)

These results show that STN DBS could replace levodopa and allowed all antiparkinsonian medication to be discontinued in 50% of patients with PD. Fluctuations and dyskinesia disappeared completely in these patients but persisted in those still on medication. These improvements were maintained for 2 years.
Genetic associated complications of type 2 diabetes mellitus (2021)

According to the International Diabetes Federation, the number of adults (age of 20-79) being diagnosed with diabetes mellitus (DM) have increased from 285 million in year 2009 to 463 million in year 2019 which comprises of 95% type 2 DM patient (T2DM). Research have claimed that genetic predisposition could be one of the factors causing T2DM complications. In addition, T2DM complications cause an incremental risk to mortality. Therefore, this article aims to discuss some complications of T2DM in and their genetic association. The complications that are discussed in this article are diabetic nephropathy, diabetes induced cardiovascular disease, diabetic neuropathy, diabetic foot ulcer (DFU) and Alzheimer's disease (AD). According to the information obtained, genes associated with diabetic nephropathy (DN) are gene GABRR1 and ELMO1 that cause injury to glomerular. Replication of genes FRMD3, CARS and MYO16/IRS2 shown to have link with DN. The increase of gene THBS2, NGAL, PIP, TRAF6 polymorphism, ICAM-1 encoded for rs5498 polymorphism and C667T increase susceptibility towards DN in T2DM patient. Genes associated with cardiovascular diseases are adiponectin gene (ACRP30) and apolipoprotein E (APOE) polymorphism gene with ξ2 allele. Haptoglobin (Hp) 1-1 genotype and mitochondria superoxide dismutase 2 (SOD2) plays a role in cardiovascular events. As for genes related to diabetic neuropathy, janus kinase (JAK), mutation of SCN9A and TRPA1 gene and destruction of miRNA contribute to pathogenesis of diabetic neuropathy among T2DM patients. Expression of cytokine IL-6, IL-10, miR-146a are found to cause diabetic neuropathy. Besides, A1a16Va1 gene polymorphism, an oxidative stress influence was found as one of the gene factors. Diabetic retinopathy (DR) is believed to have association with monocyte chemoattractant protein-1 (MCP-1) and insulin-like growth factor 1 (IGF1). Over-expression of gene ENPP1, IL-6 pro-inflammatory cytokine, ARHGAP22's protein rs3844492 polymorphism and TLR4 heterozygous genotype are contributing to significant pathophysiological process causing DR, while research found increases level of UCP1 gene protects retina cells from oxidative stress. DFU is manifested by slowing in re-epithelialization of keratinocyte, persistence wound inflammation and healing impairment. Re-epithelialization disturbance was caused by E2F3 gene, reduction of Tacl gene encoded substance P causing persistence inflammation while expression of MMp-9 polymorphism contributes to healing impairment. A decrease in HIF-1a gene expression leads to increased risk of pathogenesis, while downregulation of TLR2 increases severity of wound in DFU patients. SNPs alleles has been shown to have significant association between the genetic dispositions of T<inf>2</inf>DM and AD. The progression of AD can be due to the change in DNA methylation of CLOCK gene, followed with worsening of AD by APOE4 gene due to dyslipidemia condition in T2DM patients. Insulin resistance is also a factor that contributes to pathogenesis of AD.
Serum levels of soluble major histocompatibility complex (MHC) class I‐related chain A in patients with chronic liver diseases and changes during transcatheter arterial embolization for hepatocellular carcinoma (2008)

Soluble forms of major histocompatibility complex (MHC) class I-related chain A and B (MICA/B) are increased in the sera of patients with malignancy and impair the antitumor immune response by downregulating expression of their cognate immunoreceptor natural killer group 2, member D (NKG2D). Recently, soluble MICA/B were reported to appear even in some premalignant diseases, raising questions about the impact of soluble MICA/B produced from tumors on the expression of NKG2D. The present study examined soluble MICA/B in chronic liver disease and hepatocellular carcinoma (HCC) and their involvement in the immune-cell expression of NKG2D during transcatheter arterial embolization for HCC. The levels of soluble MICA/B were significantly higher in chronic liver disease and HCC patients than in healthy volunteers. The progression of liver disease and that of the tumor were independent determinants for soluble MICA/B levels. Immunohistochemistry revealed that MICA/B were expressed not only in HCC tissue but also on hepatocytes in cirrhotic livers. The transcatheter arterial embolization therapy significantly decreased serum levels of soluble MICA, but not soluble MICB, and increased the NKG2D expression on natural killer cells and CD8-positive T cells; there was an inverse correlation between changes in soluble MICA levels and in NKG2D expression. In conclusion, although soluble MICA/B are produced from both HCC and premalignant cirrhotic livers, therapeutic intervention for HCC can reduce the levels of soluble MICA and thereby upregulate the expression of NKG2D. Cancer therapy may have a beneficial effect on NKG2D-mediated antitumor immunity.
Imaging correlography applied to high resolution retinal imaging (2007)

The resolution of the images obtained from the eye fundus are limited by the ocular aberrations. As most of the aberrations are due to the eye optics, they do not affect the light intensity measured in the eye iris plane. By illuminating the retina with a laser and collecting the light in a pupil plane conjugate, it is possible to apply the imaging correlography technique. From processing series of pupil plane images, this technique gives information about the retina in the form of the squared modulus of the Fourier transform or, equivalently, the autocorrelation of the diffraction-limited image intensity. Two factors make this technique suitable for retinal imaging: 1) For this technique to work, changes of phase distribution in the retinal plane are necessary between each frame. Small eye movements naturally provide these changes; 2) This method does not provide directly the phase of the Fourier transform. Therefore it is of most use for centro-symmetric objects like the retina's photoreceptor mosaic. Preliminary data have been obtained <i>in vivo</i> showing the feasibility of applying such a technique in the eye. Experimental results are compared against simulation based on retinal scattering model.
Tracking and Data Relay Satellite System (TDRSS) Range and Doppler Tracking System Observation Measurement and Modeling (1980)

This document gives detailed descriptions of the tracking services, signal generation and processing, range and Doppler extraction, and the principles and procedures involved in modeling range and Doppler observations via the Tracking and Data Relay Satellite System (TDRSS). Major topics discussed include the following: TDRSS telecommunication services, functional description of the TDRSS, tracking signal generation and processing, range and Doppler observations modeling, angular observed and computed measurement algorithms, and tracking data transmission format.
Aiding Manipulation of Handwritten Mathematical Expressions through Style-Preserving Morphs (2001)

We describe a technique for enhancing a user's ability to manipulate hand-printed symbolic information by automatically improving legibility and simultaneously providing immediate feedback on the system's current structural interpretation of the information. Our initial application is a handwriting-based equation editor. Once the user has written a formula, the individual hand-drawn symbols can be gradually translated and scaled to closely approximate their relative positions and sizes in a corresponding typeset version. These transformations preserve the characteristics, or style, of the original user-drawn symbols. In applying this style-preserving morph, the system improves the legibility of the user-drawn symbols by correcting alignment and scaling, and also reveals the baseline structure of the symbols that has been inferred by system. We performed a preliminary user study that indicates that this new method of feedback is a useful addition to a conventional interpretive interface. We believe this is because the style preserving morph makes it easier to understand the correspondence between the original input and interpreted output than methods that radically change the appearance of the original input.
Computed tomography arthrography of the shoulder with tin filter-based spectral shaping at 100 kV and 140 kV (2020)

Shoulder CT arthrography with Sn 100 kV substantially reduced radiation dose and image noise and maintained image contrast, compared with the conventional protocol.
Instantaneous Signal Collision Detection Using In-Band Full-Duplex: Machine Learning VS Domain-specific Knowledge (2020)

Collision detection (CD) is a key capability of carrier sense multiple access (CSMA) based medium access control (MAC) protocol. Applying CD, the transmitter can abort transmission immediately so that the power can be saved. This technique does not need the peer receiver to give feedback on whether there is a packet collision, and hence, the overall overhead is significantly low. The challenge, however, is to operate in transmit time and instantly detect the week colliding signal in the presence of strong self-interference (SI). This paper investigates two CD methods. The first technique trains a convolutional neural network (CNN) model which operates on raw baseband samples, without the need for pre-decoding. The second method treats the SI as a normal signal and estimates the signal to noise ratio (SNR): low SNR implies there is a collision because the pure SI is expected to have high SNR. Both models are evaluated by IEEE 802.15.4-like measured and simulated signals. The results show that collisions up to 30 dB below the SI signal can be detected precisely within 20 μs, while the proposed models can deliver acceptably low false alarm rate <; 1.5 %.
Human monocyte-mediated lysis of antibody-coated tumor cells: the role of the cytoskeleton in monocytes. (1985)

Human monocytes (M phi) show high cytolytic activity towards antibody-coated tumor cells (AbK562). In this report, the relationship between the cytoskeleton in the M phi and the M phi cytolytic activity has been investigated. The actin filament inhibitors cytochalasin B and dihydrocytochalasin B (H2CB) both reduced M phi-mediated lysis of AbK562 cells by approximately 50% at a concentration of 1 microM. This concentration of H2CB did not inhibit the number of target cells bound to M phi. Dihydrocytochalasin B did not inhibit the M phi ability to release cytotoxic protein factors, suggesting that H2CB does not inhibit lysis by inhibiting release of cytotoxic protein factors. Immunofluorescence microscopy showed a rapid accumulation of actin filaments towards the contact area in more than 80% of the examined M phi-AbK562 conjugates. Exposure to H2CB did not prevent this accumulation, but caused aggregation of the accumulated actin filaments in the contact area with the target cell. Accumulation of actin filaments did not occur toward tumor cells not coated with antibodies. Scanning and thin section electron microscopy demonstrated large M phi pseudopodia directed toward the AbK562 cells, with close apposition of the effector and target cell membranes with interdigitations. The formation of the M phi pseudopodia was inhibited by exposure to H2CB. These observations indicate that M phi membrane motility toward AbK562 cells is closely related to M phi-mediated lysis of AbK562 cells. Immunofluorescence microscopy of the microtubule-organizing center (MTOC) and the Golgi apparatus revealed that both the MTOC and the Golgi apparatus in M phi reoriented towards the bound AbK562 cells in approximately 45% of the examined M phi-AbK562 conjugates. The microtubule-depolymerizing drugs colchicine and vinblastine did not inhibit M phi-mediated lysis of AbK562 cells at concentrations which disrupted the microtubule arrays in the M phi. The carboxylic ionophore monensin, which blocks Golgi-derived secretion, inhibited M phi-mediated lysis of AbK562 to a lesser extent as compared to H2CB. These results suggest that microtubule functions are of less importance in M phi-mediated lysis of AbK562 cells as compared to actin filament functions. However, the MTOC and the Golgi apparatus could participate in M phi-mediated lysis of AbK562 cells by mechanisms related to secretion of cytotoxic molecules.
Erratum: Hepatic Dendritic Cells, the Tolerogenic Liver Environment, and Liver Disease (2018)

Correction to: Hepatic Dendritic Cells, the Tolerogenic Liver Environment, and Liver DiseaseSemin Liver Dis 2018; 38(02): 170-180DOI: 10.1055/s-0038-1646949
Interaction between Copper Chlorophyllin and Poly(vinyl alcohol) (1993)

Abstract The difference spectrum between copper chlorophyllin (Cu-chln) plus poly(vinyl alcohol) (PVA) and Cu-chln alone exhibits a maximum at 652 nm in water. The Benesi-Hildebrand plots give equilibrium constants of 833 mol−1 dm3 for UMR-20H (degree of saponification: 81.7%) and 1820 mol−1 dm3 for UMR-20M (degree of saponification: 63.8%). The Scatchard plot of these data shows the existence of a cooperative binding.
Metabolic defects induced by high-fat feeding in mice are rapidly reversed by a low-fat diet (2012)

Results Mice fed the HFD displayed a 73% increase (P<0.01) in whole-body fat mass, an 80% elevation (P<0.01) in muscle and liver triglyceride levels and a substantial impairment in glucose tolerance compared to animals fed the LFD (area under curve during GTT: 1166 ± 76 vs. 506 ± 47 mM.min, P<0.001). The switch to a LFD resulted in a transient decrease in total caloric intake, but only a small drop in body weight (31.3 ± 0.8 vs. 30.3 ± 0.5g, pre vs. post, P=0.06). Despite the minimal change in body weight, whole-body fat mass in the HFD-LFD group was reduced almost to the level of LFD controls (12.7% vs. 14.2% by DXA, LFD vs. HFD-LFD). Consistent with the reduction in whole-body adiposity, glucose tolerance (AUC during GTT: 510 ± 49 mM.min) and muscle and liver triglycerides in the HFD-LFD animals were also restored to the level of LFD animals. Intriguingly, a separate group of mice that were pair-fed HFD to match the drop in caloric intake in the HFD-LFD group, displayed no improvements in glucose tolerance or tissue triglyceride levels.
[Infection of the newborn infant by the human immunodeficiency virus]. (1988)

HIV infection of the newborn is now known to result mostly from mother-to-foetus transmission. The risk of transmission is at least 40 p. 100. However, the circumstances of passage are little known, and there is no maternal virological parameter capable of evaluating individual risks. The disease is more severe in children than in adults. Rare are the children who remain asymptomatic for more than 15 months; one out of three of them develop severe acquired immunodeficiency syndrome and die within the first 2 or 3 years of life. A specific encephalopathy is observed in about 30 p. 100 of the infected children. Kaposi's sarcoma is exceptional. Since there is no contagion between children, those who are in fairly good clinical condition should have a family and school life as normal as possible.
Investigation of Deterioration of Joints in Concrete Pavements: Field Study of Penetrating Sealers (2016)

The objective of this research was to assess the efficacy of various waterproofing sealers applied to pavement joints with respect to limiting water ingress. The measure of water ingress was the chloride concentration profile as measured by scanning electron microscopy and energy dispersive spectroscopy. The fieldwork was conducted at the MnROAD facility. Cores were retrieved from pavements to assess the before condition. Various silane- and siloxane-based sealers were applied in 2013, and the pavements were exposed to service for two years. After two years, cores were retrieved. The chloride profiles for various pavement sites were compared before and after application of the sealer. No appreciable differences were noted. The lack of measured differences is attributed to the short time span allowed for ingress. The older pavements measured had a considerable degree of chloride ingress and, as such, small changes were difficult to detect. The newer pavement analyzed also showed no appreciable change, but it would be worth re-analyzing after more time has elapsed (e.g., two more years).
Efficient and accurate investigation of linear arrays consisting of UWB radiators (2009)

The linear array performance of the wide-band CPW-fed printed “Eared” antennas is investigated by means of simulations. The elements behave well in the array environment. A method is described to extrapolate the (active) reflection coefficient and radiation characteristics of any array consisting of more than 7 elements by using the simulated results of a 1 by 7 array. The results confirm that there is no need to simulate the whole array if the number of elements in the array exceeds 7, this resulting in sizable computation time saving.
Think for Yourself. Your Middle School Classroom. (2005)

We study the diffusion-driven destabilization of a spatially homogeneous limit cycle with large amplitude in a reaction-diffusion system on an interval of finite size under the periodic boundary condition. Numerical bifurcation analysis and simulations show that the spatially homogeneous limit cycle becomes unstable and changes to a stable spatially nonhomogeneous limit cycle for appropriate diffusion coefficients. This is analogous to the diffusion-driven destabilization (Turing instability) of a spatially homogeneous equilibrium. Our approach is based on a reaction-diffusion system with mass conservation and its perturbed system considered as an infinite dimensional slow-fast system (relaxation oscillator).