gebhart/patrepeval huggingface dataset:
Testin knock-out mice reveal a tumor suppressor function in chemically-induced forestomach carcinogenesis (2005)

1983 Loss of Hetherozygosity (LOH) at a given chromosomal fragile site is associated with the loss of tumor suppressor genes (TSG). One of these regions is located on the long arm of chromosome 7 and it is deleted in epithelial cancers as well as in hematopoietic malignancy. At 7q31.2 we have identified the Testin (Tes) gene as a putative TSG for this region. Tes encodes for a LIM domain protein that plays a role in cell adhesion. The protein is mainly localized to the tip of actin stress fibers at focal adhesion plaques and cell-cell-contact areas: its overexpression seems to affect cell growth, cell spreading and cell motility. To test whether Testin displays a tumor suppressor role in vivo we have generated a Tes knock-out mouse and we have assessed its role in a well established gastric carcinogenic model. In mice a zinc deficient diet (ZD) enhances cellular proliferation in forestomach and susceptibility to N-nitrosomethylbenzylamine (NMBA) induced carcinogenesis. Five week old Tes wild type (+/+), heterozygous (+/−) and homozygous (−/−) mice have been homogenously distributed into four groups: zinc sufficient diet fed mice (ZS), zinc deficient diet fed mice (ZD), zinc sufficient fed+NMBA treated mice (ZS+NMBA), zinc deficient fed+NMBA treated mice (ZD+NMBA). After four weeks from the beginning of the study, the ZS+NMBA and ZD+NMBA groups were treated with three intragastric doses of NMBA (2 mg/kg body weight, twice a week). Animals were all sacrificed eight weeks after NMBA administration to assess the tumor incidence in each group for each genotype. In the ZS+NMBA group we have found mostly a mild proliferative epithelium and small papillomas in 8/14 cases (57.1%) of the +/+ mice, large papillomas and dysplasia in 19/21 cases (90.4%) of the +/− mice and large papillomas, adenomas and carcinomas in 20/21 cases (95.2%) of the −/− mice. The ZD+NMBA group has shown only benign lesions in 8/15 cases (53.3%) of +/+ mice, multiple very large papillomas, atypical glandular metaplasia and carcinomas in 29/30 cases (96.6%) of +/− mice, very large papillomas, squamous cell carcinomas and invasive carcinomas in 16/16 cases (100%) of −/− mice. A statististical significant difference was found in the ZS+NMBA group between +/+ versus +/− (P=0,038) and +/+ versus −/− (P=0,01) mice as well as in the ZD+NMBA group between +/+ versus +/− (P=0,006) and +/+ versus −/− (P=0,0009) mice. We are still studying the spontaneous tumor phenotype in the Tes +/+, +/− and −/− mice. Our results provide the the first evidence that Testin functions as a tumor suppressor gene in vivo.
<title>Crystal structures of metal complex sensitizers of TiO<formula><inf><roman>2</roman></inf></formula> nanopowders</title> (1997)

The x-ray structure determination of three efficient Ru complex sensitizers of TiO<SUB>2</SUB> nanopowders was carried out. The coordination of Ru was analyzed in terms of asymmetric ligand surrounding of metal atom and influence of chelate (pi) -conjugated ligands on the bonding of Ru with ligands and on the intermolecular association of complexes. The layered character of all three studied crystal structures could be correlated with the peculiarities of their molecular structures (polarity, presence of (pi) -conjugated moieties), what opens the possibility of using of molecular engineering methods to influence the anchoring and ordering of sensitizer molecules on semiconductor surfaces.
Caseins and Casein Hydrolysates. 1. Lipoxygenase Inhibitory Properties (2000)

Whole casein from bovine origin, the different casein subtypes alpha, beta, and kappa, and the related dephosphorylated proteins were assayed as modulators of soybean lipoxygenase 1 activity and were found to inhibit it. To define the lipoxygenase inhibitory domain, whole casein and beta-casein were digested by proteases (trypsin, clostripain, and subtilisin). The beta-casein tryptic digest and the tryptic and subtilisin digests of whole casein retained their inhibitory properties. The tryptic beta-casein digest was the most potent inhibitor of lipoxygenase activity and was further fractionated by FPLC or HPLC. The collected peptides inhibited the lipoxygenase-catalyzed reaction to different extents. The active fractions were analyzed by ESI-MS, and the sequences of several lipoxygenase inhibitory peptides, corresponding mainly to the C-terminal moiety of beta-casein, were identified.
4-Hydroxy-2-hydroxymethylcyclopent-2-en-1-one: a versatile intermediate for the synthesis of cyclopentanoid natural products (1980)

J. D. Elliott, M. Hetmanski, R. J. Stoodley and M. N. Palfreyman, J. Chem. Soc., Chem. Commun., 1980, 924 DOI: 10.1039/C39800000924
Amperometric biosensors for detection of L‐ and D‐amino acids based on coimmobilized peroxidase and L‐ and D‐amino acid oxidases in carbon paste electrodes (1994)

Abstract An apparent direct electron transfer between various electrode materials and peroxidases immobilized on the surface of the electrode has been reported in the last few years. An electrocatalytic reduction of hydrogen peroxide promoted by the immobilized peroxidase starts at about +600 mV (vs. Ag/AgCl) at neutral pH. The efficiency of the electrocatalytic current increases as the applied potential is made more negative and starts to level off it about −100 mV (vs. Ag/AgCl). Amperometric biosensors for hydrogen peroxide can be constructed with these kinds of peroxidase modified electrodes. By coimmobilizing a hydrogen peroxide‐producing oxidase with the peroxidase, amperometric biosensors can be made responding to the substrate of the oxidase within a potential range essentially free of interfering electrochemical reactions. Sensors for L‐ and D‐amino acids are shown based on coimmobilizing HRP with L‐amino acid oxidase (L‐AAOD) or D‐amino acid oxidase (D‐AAOD). The effects of the immobilization procedure, buffer (flow carrier), pH, taken amounts of enzymes, flow rate, and injection volume on the response were investigated. The addition of polyethylenimine (PEI) into the paste was found to increase the response considerably. All 20 of the most common L‐amino acids could be detected.
Distribution of Diagonal Elements of a General Central Complex Wishart Matrix (2012)

We consider a general central complex Wishart matrix derived from non-circular complex Gaussian vectors. We present a technique for evaluation of the probability density, cumulative distribution, and moment generating functions of the joint distribution of the diagonal elements of the Wishart matrix. We give examples of application of the obtained results.
Complexity in wireless scheduling (2008)

It has been an important research topic since 1992 to maximize stability region in constrained queueing systems, which includes the study of scheduling over wireless ad hoc networks. In this paper, we propose a framework to study a wide range of existing and future scheduling algorithms and characterize the achieved tradeoffs in stability, delay, and complexity. These characterizations reveal interesting properties hidden in the study of any one or two dimensions in isolation. For example, decreasing complexity from exponential to polynomial, while keeping stability region the same, generally comes at the expense of exponential growth of delays. Investigating trade-offs in the 3-dimensional space allows a designer to fix one dimension and vary the other two jointly. For example, incentives for using scheduling algorithms with only partial throughput-guarantee can be quantified with regards to delay and complexity. Trade-off analysis is then extended to systems with congestion control through utility maximization for non-stabilizable arrival inputs, where the complexity-utility-delay trade-off is shown to be different from the complexity-stability-delay tradeoff. Finally, we analyze more practical models with bounded message size, and consider "effective throughput" which reflects resource occupied by control messages. We show that effective throughput may degrade significantly in certain scheduling algorithms, and suggest a mechanism to avoid this problem in light of the 3D tradeoff framework.
Selective Synthesis of 1-Functionalized-alkyl-1H-indazoles (2009)

An efficient method for the selective "N1" alkylation of indazoles is described. Use of alpha-halo esters, lactones, ketones, amides, and bromoacetonitrile provides good to excellent yield of the desired N1 products.
Supplemental Dataset 1 from AXL Inhibition Sensitizes Mesenchymal Cancer Cells to Antimitotic Drugs (2023)

&lt;p&gt;Supplemental Dataset 1. Expression of AXL, GAS6, Vimentin and E-Cadherin in 644 human cancer cell line using RNAseq technology.&lt;/p&gt;
Force and Hydraulic Displacement Amplification of Fiber Reinforced Soft Actuators (2013)

Soft robots allow for complex continuum motions and shapes that conform to their environment. Using a fiber-reinforced elastomeric enclosure (FREE) driven by fluid provides a high power density, soft continuum actuator. While the force generation for a small subset of this structure known as McKibben actuators has been studied extensively, the force and moments generated by a wider set of fiber reinforcements have not been previously investigated. Using virtual work and kinematics derived from fiber inextensibility and fluid incompressibility, the force and moments for the entire design space of FREEs has been determined analytically. Graphical representations have been created, providing easy tools for synthesis and analysis of force and moments in all possible FREEs. The hydraulic displacement amplification, or volumetric transduction, of output motion to fluid displacement has also been determined using kinematics; this transduction gives an indication of stiffness of the structure. Graphical representations have also been created, providing a designer with an intuitive understanding of the behavior all FREE topologies.
Desensitization of the P2X2 receptor controlled by alternative splicing (1997)

P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X(2) receptor splice variant (P2X(2-2)) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X(2) gene. P2X(2-2) is as highly expressed as the original P2X(2) sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X(2) or P2X(2-2) receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X(2) isoforms.
Cardiometabolic Health After Pediatric Cancer Treatment: Adolescents Are More Affected than Children (2022)

This cross-sectional study aimed at comparing the cardiometabolic (CM) health of children and adolescents and identifying factors associated with CM complications shortly after cancer treatment. Cancer-related characteristics, blood pressure (BP), anthropometry, and biochemical parameters were collected in 80 patients (56.3% female, mean age: 11.8 years; range: 4.5 - 21.0) a mean of 1.4 years following therapy completion. Compared to children, adolescents had higher mean <i>z</i>-score of insulin (-0.47 vs. 0.20; <i>P</i> = 0.01), HOMA-IR (-0.40 vs. 0.25; <i>P</i> = 0.02), waist-to-height ratio (0.36 vs. 0.84; <i>P</i> = 0.01), subscapular skinfold thickness (-0.19 vs. 0.47; <i>P</i> = 0.02), total body fat (-1.43 vs. 0.26; <i>P</i> < 0.01), and lower mean <i>z</i>-score of HDL-C (0.07 vs. -0.53; <i>P</i> < 0.01). Adolescents were more likely to have high BP (42% vs. 15%; <i>P</i> < 0.01), dyslipidemia (64% vs. 15%; <i>P</i> < 0.001), and cumulating ≥ 2 CM complications (42% vs. 2%; <i>P</i> < 0.001) than children. Adiposity indices (<i>z</i>-scores) were associated with high BP [odds ratio (OR) ranging from 2.11 to 4.09] and dyslipidemia (OR ranging from 2.06 to 4.34). These results suggest that adolescents have a worse CM profile than children shortly after therapy and that adiposity parameters are associated with CM complications, highliting the importance to develop intervention strategies targeting this population.
Chapter 22. Magnetic Resonance Imaging of the Brain using Hyperpolarized 129Xe (2015)

The use of laser-polarized xenon-129 (129Xe) as a novel contrast agent for magnetic resonance imaging (MRI) is quite useful for imaging the lungs and other organs such as the brain. From the earliest days of HP 129Xe MRI, the original interest of one of the co-inventors (Dr Mitchell Albert) was to use 129Xe to better understand the brain and directly image the effect of anaesthesia on brain function. Since xenon is a known anaesthetic and 129Xe is a spin 1/2 nucleus it was a logical choice to study the effects of anaesthesia on the brain using MRI. In this chapter, we will briefly review some historical advancements for functional brain imaging, such as functional magnetic resonance imaging (fMRI) and the use of 129Xe for animal and human brain imaging. The practical considerations for imaging the human brain using magnetic resonance will also be discussed. Considerations include: hyperpolarized gases, physical and chemical properties of xenon, routes and methods of delivery, physiological effects and patient safety. Finally, some basic ground-work and recent literature in the development of brain imaging using HP 129Xe in animals and humans will be discussed.
Mechanism of Action and Capsid-Stabilizing Properties of VHHs with an In Vitro Antipolioviral Activity (2014)

The study describes the mechanism of neutralization and the capsid-stabilizing activity of five single-domain antibody fragments (VHHs) that have an in vitro neutralizing activity against poliovirus type 1. The results show that the VHHs interfere at multiple levels of the viral replication cycle (cell attachment and viral uncoating). These mechanisms are possibly shared by some conventional antibodies and may therefore provide some insight into the natural immune responses. Since the binding sites of two VHHs studied by cryo-EM are very similar to that of the receptor, the VHHs can be used as probes to study the authentic virus-cell interaction. The structures and conclusions in this study are original and raise interesting findings regarding virus-receptor interactions and the order of key events early in infection.
Increased Risk of Venous Thromboembolism With Everolimus-Based Immunosuppression in Lung Transplant Recipients: A Case-Controlled Study. (2014)

Background: Everolimus (EVR) is used in lung transplant recipients (LTxR) for its renal sparing, anti-fibrotic and anti-cancer effects. Sirolimus, the parent compound of EVR, is associated with an increased risk of venous thromboembolism (VTE) in LTxR. We aimed to determine if EVR increased VTE risk in LTxR. Methods: We performed a retrospective, case-control study of LTxR who received EVR at our center. Controls were matched with LTxR on EVR based on time post-LTx and transplant indication. The primary outcome was the proportion of LTxR with VTE [deep venous thrombosis (DVT), pulmonary embolism (PE) or stroke]. Equal time periods (from time of EVR initiation) were assessed for each LTxR on EVR and accompanying control. LTxR receiving warfarin, or with a history of VTE or myocardial infarction were excluded. Fisher's exact and the t tests were used for categorical and continuous variables, respectively. Results: 55 EVR LTxR and 55 controls were included. The mean time evaluated for VTE was 588 ± 402 days for each LTxR. There was a higher proportion VTE in the EVR group [9 (16%) vs. 1 (2%), p=0.02].Table: No Caption available.F=female, M=male, ILD=interstitial lung disease, A1A=alpha-1 antitrypsin deficiency, COPD=chronic obstructive pulmonary disease, UE=upper extremity, LE=lower extremity There was no significant difference in age, gender or LTx type (single vs. double) between groups; however, there were more hospitalizations in the EVR group (1.4 ± 1.5 vs. 0.8 ± 1.4 per LTxR, p=0.05). Three VTE events were intravenous line-associated (all UE DVTs in the EVR group). None of the LTxR with VTE had a surgery requiring hospitalization in 90 days preceding their VTE. Conclusion: EVR was associated with a higher risk of VTE in LTxR in this analysis. EVR may trigger VTE via a class effect of sirolimus and EVR on plasminogen activator inhibitor-1 expression. A more robust analysis, which controls for co-founders, is required to confirm these findings. DISCLOSURES:Schoeppler, K.: Other, Novartis, Grant funds. Zamora, M.: Other, Novartis, Grant Funds.
Spectral and calorimetric studies of hydrogen bonding with pyrrole (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectral and calorimetric studies of hydrogen bonding with pyrroleRussell S. Drago and M. S. NozariCite this: J. Am. Chem. Soc. 1970, 92, 24, 7086–7090Publication Date (Print):December 1, 1970Publication History Published online1 May 2002Published inissue 1 December 1970https://pubs.acs.org/doi/10.1021/ja00727a012https://doi.org/10.1021/ja00727a012research-articleACS PublicationsRequest reuse permissionsArticle Views158Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Affinity Chromatography (2019)

Recommendations are given concerning the terminology of methods of separation in analytical chemistry, including chromatography, electromigration techniques, and field-flow fractionation and related techniques.
Supplementary Figure 1 from Sirtuin 1 Is Upregulated in a Subset of Hepatocellular Carcinomas where It Is Essential for Telomere Maintenance and Tumor Cell Growth (2023)

Supplementary Figure 1 from Sirtuin 1 Is Upregulated in a Subset of Hepatocellular Carcinomas where It Is Essential for Telomere Maintenance and Tumor Cell Growth
Stromal Progenitor Cells from Endogenous Adipose Tissue Contribute to Pericytes and Adipocytes That Populate the Tumor Microenvironment (2012)

Epidemiologic studies associate cancer with obesity, but the pathophysiologic connections remain obscure. In this study, we show that obesity facilitates tumor growth in mice irrespective of concurrent diet, suggesting a direct effect of excess white adipose tissue (WAT). When transplanted into mice, adipose stromal cells (ASC) can serve as perivascular adipocyte progenitors that promote tumor growth, perhaps helping explain the obesity-cancer link. In developing this hypothesis, we showed that ASCs are expanded in obesity and that they traffic from endogenous WAT to tumors in several mouse models of cancer. Strikingly, a comparison of circulating and tumor-infiltrating cell populations in lean, and obese mice revealed that cancer induces a six-fold increase of ASC frequency in the systemic circulation. We obtained evidence that ASCs mobilized in this way can be recruited into tumors, where they can be incorporated into blood vessels as pericytes and they can differentiate into adipocytes in an obesity-dependent manner. Extending this evidence, we found that increased tumor vascularization (reflected by changes in tumor vascular morphology and a two-fold increase in vascular density) was associated with intratumoral adipocytes and elevated proliferation of neighboring malignant cells. Taken together, our results suggest that ASCs recruited from endogenous adipose tissue can be recruited by tumors to potentiate the supportive properties of the tumor microenvironment.
Figure S3 from ADAMDEC1 Maintains a Growth Factor Signaling Loop in Cancer Stem Cells (2023)

&lt;p&gt;Supplementary Figure S3&lt;/p&gt;
Adaptação e validação do CEQ – Questionário de Experiência no Curso – Contributo para a qualidade do ensino superior português (2012)

A investigação sobre a aprendizagem no ensino superior estabeleceu uma associação entre a forma como os estudantes aprendem e a percepção que têm sobre os contextos em que decorre essa aprendizagem. Tendo em conta a relevância desta componente para a qualidade da aprendizagem no ensino superior o objectivo deste estudo é identificar a estrutura factorial do CEQ - Questionário de Experiência no Curso (Ramsden, 2006) validando-o para a população estudantil portuguesa. A partir de uma amostra de 872 alunos de cursos de Licenciatura (Ciências Sociais e Ciências e Tecnologia) testámos a adequabilidade global do modelo à estrutura relacional desenvolvida, com recurso à Análise Factorial Confirmatória. A principal conclusão indica que o modelo se ajusta à população estudantil portuguesa, embora com algumas diferenças, também encontradas em estudos transculturais realizados recentemente
A Modified Cryo-Jaw for in Vitro Biomechanical Testing of Tendons (2002)

The purpose of this study was to develop a new device, which represents a modification of the Cryo-Jaw described by Riemersa and Schamhardt and modified by Hamner et al., for in vitro biomechanical testing of tendons which allows the lower clamp to move in every direction and thus simulate a pathological dislocation of the knee. Tendons are fixed to the device by freezing the clamped part with dry ice. After fixation of their free ends, the lower clamp was rotated 45°, translated 1 cm, and angled 40° to simulate a knee sprain. Various configurations of bundles were tested: parallel, twisted, and braided. Tests were performed on 10 paired bovine bifurcated digital extensor tendons and 6 paired human hamstring tendons. Grafts were then tested to failure subjected to impulsive load, using a servohydraulic machine. The highest ultimate load recorded for parallel bundles was 4662 ± 565.71 N for bovine bifurcated digital extensor tendons, and 3057 ± 475.44 N for human hamstring tendons. In any case, the tendons ruptured midway, well clear of the frozen part; in no case was slippage of the tendons observed. Thus the device proposed allows one to test what happens to the graft of an ACL reconstructed knee during physiological and pathological movements because it can be easily displaced in every direction.
A 19.5 GHz 28 nm CMOS Class-C VCO with Reduced 1/f Noise Upconversion (2019)

Class-C operation is leveraged to implement a K-band CMOS VCO where the upconversion of the 1/f noise from the core transistors is robustly contained at a minimal level. Implemented in a bulk 28 nm CMOS technology, the VCO shows a phase noise as low as -108.5 dBc/Hz at 1 MHz offset (-83 dBc/Hz at 100 kHz offset) from the 19.5 GHz carrier, while consuming 14.4 mW and featuring a 12% tuning range.
Preparation of BCYF0.10–YDC/BCYF0.10–Ni dual-layer hollow fiber membrane for dry reforming of methane and hydrogen purification (2023)

A ceramic hydrogen permeable membrane reactor for dry reforming of methane (DRM) enables both the reaction and separation processes to be carried out simultaneously, producing synthesis gas and pure hydrogen while mitigating greenhouse gas emissions.