gebhart/patrepeval huggingface dataset:
In vitro Embryo Production in Llamas (Lama glama) from In vivo Matured Oocytes with Raw Semen Processed with Androcoll‐E using Defined Embryo Culture Media (2011)

The aim of this study was to carry out in vitro fertilization using spermatozoa selected with Androcoll-E™ and to evaluate the efficiency of the culture medium DMEM-F12 for in vitro embryo development in the llama. Twelve adult females from 18 superstimulated (67%) were used as oocyte donors. They were superstimulated with 1500 IU of eCG and after 5 days, received a single dose of buserelin. Twenty hours post-injection, follicular aspiration was conducted by flank laparotomy. Semen collections were performed under general anesthesia by electroejaculation of the male. The ejaculates were processed with a solution of collagenase (0.1%) and an Androcoll-E™ column was used to improve the sample. Sixty nine COCs were recovered from 79 aspirated follicles (87% recovery). Only expanded COCs were used (n = 67); they were randomly placed in groups of 1-5 in Fertil-TALP and the sperm suspension (20 × 10(6) live spermatozoa/ml) was added to each fertilization microdroplet. After 24 h, they were randomly placed in one of two culture media: SOF (n = 34) or DMEM-F12 (n = 33) and incubated for 6 days in humidified atmosphere of 5% CO(2) , 5% O(2) and 90% N(2) at 38°C. The blastocyst rate was 20% (7/34) in SOF medium (3 hatched, 2 expanded and 2 early blastocysts) and 15% (5/33) in DMEM medium (all expanded blastocysts). In conclusion, using Androcoll-E™ it is possible to select good quality spermatozoa from llama ejaculates for in vitro fertilization and to produce blastocysts in DMEM-F12 medium. This is also the first time that hatched llama blastocysts have been produced after culture in a defined medium such as SOFaa.
Statistical Analysis of Ion Mobility Spectrometry. I. Unbiased and Guided Replica-Exchange Molecular Dynamics (2011)

Achieving (bio)macromolecular structural assignment from the interpretation of ion mobility spectrometry (IMS) experiments requires successful comparison with computer modeling. Replica-exchange molecular dynamics simulations with suitable force fields not only offer a convenient framework to locate relevant conformations, especially in the case of multiple-funnel energy landscapes, but they are also well suited to statistical analyses. In the present paper, we discuss two extensions of the method used to improve its efficiency in the context of IMS. Two doubly-protonated polyalanines [RA(4)XA(4)K + 2H](2+) with X=V and D appear as favorable cases for which the calculated collision cross-section distributions naturally agree with the measurements, providing reliable candidate structures. For these compounds, a careful consideration of other order parameters based on the weighted histogram method resolves several otherwise hidden underlying conformational families. In the case of a much larger peptide exhibiting bistability, assignment is more difficult but could be achieved by guiding the sampling with an umbrella potential using the square gyration radius as the biasing coordinate. Applied to triply protonated bradykinine, the two presented methods indicate that different conformations compatible with the measurements are very close in energy.
Novel Active Slug Control in Angola - Development & Field Results (2021)

Abstract Slug flow in multiphase flowlines can cause operational instabilities which, when severe, lead to mechanical damage or even shutdown of processing facilities. While a number of control schemes to handle slugging have been published, many of them require subsea instrumentation or make use of calculated "pseudo-variables" for control, values which have no real physical meaning. Hydrodynamic slugging was anticipated during design of a new facility in Angola, and a simulation study demonstrated that a control scheme from the published literature could be applied effectively to control the slugging. However, that solution was rejected because of the use of a pseudo-variable as the principal control point. Therefore, a novel control scheme was developed and tested on simulation for both hydrodynamic slugging and severe riser-induced slugging and later patented.(1,2) The project implemented the novel active slugging control using a topsides control valve and topsides instrumentation. While a pseudo-variable, a pseudo-flow controller, was employed, it was part of a cascade scheme such that the principal control variable was a real topside pressure measurement. Upon commissioning, slugging at the facility was found to be more severe than anticipated during design, but the novel active slug control scheme was effective in controlling incoming slugs.
Treatment of Schizophrenia (1987)

Antipsychotic medication remains a mainstay of treatment in both acute and chronic schizophrenia. Emphasis in recent years has focused on maximizing benefits and minimizing risks of medication by attempting to establish minimum effective dosage requirements for all phases of treatment and to provide alternative strategies for individuals who fail to benefit from antipsychotic drug treatment. At present all approaches to the treatment-refractory patient remain experimental, and further research in this area is of critical importance. Definite advances have been made in exploring the impact of psychological and psychosocial treatments administered in conjunction with various antipsychotic drug strategies. More sophisticated and comprehensive assessment measures have been applied in long-term treatment trials, enabling us to be more specific about treatment goals and treatment evaluation. Although no major "breakthrough" has occurred in the treatment of schizophrenia, incremental advances which can reduce rates of relapse and rehospitalization, improve the quality of adaptation, and reduce the risk of significant adverse effects are of enormous importance to affected individuals, their families, and society at large.
Computation of optical flow using basis functions (1997)

The issues governing the computation of optical flow in image sequences are addressed. The trade-off between accuracy versus computation cost is shown to be dependent on the redundancy of the image representation. This dependency is highlighted by reformulating Horn's (1986) algorithm, making explicit use of the approximations to the continuous basis functions underlying the discrete representation. The computation cost of estimating optical flow, for a fixed error tolerance, is shown to be a minimum for images resampled at twice the Nyquist rate. The issues of derivative calculation and multiresolution representation are also briefly discussed in terms of basis functions and information encoding. A multiresolution basis function formulation of Horn's algorithm is shown to lead to large improvements in dealing with high frequencies and large displacements.
Security vulnerabilities in modern web browser architecture (2010)

Abstract- The Web today has become the most used and popular platform for application development. In the beginnings of the Web, applications provided users just ability to browse and read content. The expansion and adoption of the new web technologies has led to a significant increase in development and, more importantly, usage of the web applications that allow users to create their own content and impact their life (e.g. e-banking, e-commerce, social networks). Web 2.0 applications introduced new possibilities for both users and application developers, but also created new security concerns. Almost every Internet user uses a web browser to access any content on the Internet. Each web application is designed and developed to be executed inside the web browser. Web browser mediates between users and applications. In such architecture, malicious applications could be loaded and executed inside the web browser, making it a vulnerable point in preserving security. Modern web applications demand for a new web browser architecture design that will meet new security requirements arisen with the Web 2.0. In this paper, we study web browser&amp;apos;s vulnerabilities, analyze popular web browsers architecture and present how they cope with potential security threats. I.
A clustering-based approach for prediction of cardiac resynchronization therapy (2005)

This paper presents a method for predicting pacing sites in the left ventricle of a heart and its result can be used to assist device programming in cardiac resynchronization therapy (CRT), which is a widely adopted therapy for heart failure patients. In a traditional CRT device deployment, pacing sites are selected without quantitative prediction. That runs the risk of suboptimal benefits. In this work, a surface tracking method is proposed to describe the ventricular wall motion and a hierarchical agglomerative clustering technique is applied to radial motion series to find candidate pacing sites. Using clinical MRI data in our experiments, we show that the proposed method performs as well as we expect. Our approach can not only effectively identify suitable pacing sites, but also distinguish patients from normals perfectly to help medical diagnosis.
Graded ATP depletion can cause necrosis or apoptosis of cultured mouse proximal tubular cells (1998)

The mechanisms of cell death induced by ATP depletion were studied in primary cultures of mouse proximal tubular (MPT) cells. Graded ATP depletion, ranging in severity from approximately 2 to 70% of control levels, was induced by incubating cells with either antimycin or 2-deoxyglucose, with varying concentrations of dextrose. We found that cells subjected to ATP depletion below approximately 15% of control died uniformly of necrosis. In contrast, cells subjected to ATP depletion between approximately 25 and 70% of control all died by apoptosis. The rapidity of cell death was proportional to the severity of reduction of cell ATP content and was independent of the mechanism of cell death. Renal growth factors, epidermal growth factor (EGF) and high-dose insulin, did not ameliorate apoptotic cell death induced by ATP depletion. We conclude that ATP depletion can cause either necrosis or apoptosis in MPT cells. Furthermore, we have identified a narrow range of ATP depletion (approximately 15 to 25% of control) representing a threshold that determines whether cells die by necrosis or apoptosis.
Anchor Bolts in Reinforced Concrete Foundations (1987)

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Como garantir a biossegurança na utilização dos equipamentos fotoativadores? (2021)

Já sabemos que os equipamentos fotoativadores podem ser diferentes1 e que suas características, assim como a técnica do operador, podem influenciar na reação de polimerização dos materiais resinosos2. Outro assunto que precisa ser discutido é a biossegurança, pois o uso diário do equipamento em diferentes pacientes exige uma forma de proteção correta deles, para evitar a contaminação cruzada, especialmente considerando-se a condição atual de atendimento durante a pandemia do COVID-19.
Ultrathin, ordered oxide films on metal surfaces (2008)

Metal oxides and oxide thin films are extensively used as active catalysts and catalytic supports, as well as in many other important technical applications. Unlike TiO(2), which is a semiconductor and can be investigated using a variety of surface science techniques, most metal oxides are insulators, which seriously restricts their use as model surfaces with modern surface science techniques. This difficulty can be circumvented by synthesizing ultrathin oxide films a few nanometers in thickness with well-defined structures, that mimic the corresponding bulk oxides yet are thin enough to be sufficiently conducting. In this review, preparations, structures, electronic and chemical properties of four representative oxides, alumina, magnesium oxide, silica, and titania, are addressed. Of these MgO is found to grow in a layer-by-layer fashion, allowing preparation of crystalline thin film structures with varying thicknesses. Crystalline TiO(2) and Ti(2)O(3) can also be synthesized, whereas SiO(2) and Al(2)O(3), although amenable to synthesis as well-defined monolayer structures, have only been grown to date as amorphous multilayers.
Solid-Binding Peptide-Guided Spatially Directed Immobilization of Kinetically Matched Enzyme Cascades in Membrane Nanoreactors (2021)

Biocatalysis is a useful strategy for sustainable green synthesis of fine chemicals due to its high catalytic rate, reaction specificity, and operation under ambient conditions. Addressable immobilization of enzymes onto solid supports for one-pot multistep biocatalysis, however, remains a major challenge. In natural pathways, enzymes are spatially coupled to prevent side reactions, eradicate inhibitory products, and channel metabolites sequentially from one enzyme to another. Construction of a modular immobilization platform enabling spatially directed assembly of multiple biocatalysts would, therefore, not only allow the development of high-efficiency bioreactors but also provide novel synthetic routes for chemical synthesis. In this study, we developed a modular cascade flow reactor using a generalizable solid-binding peptide-directed immobilization strategy that allows selective immobilization of fusion enzymes on anodic aluminum oxide (AAO) monoliths with high positional precision. Here, the lactate dehydrogenase and formate dehydrogenase enzymes were fused with substrate-specific peptides to facilitate their self-immobilization through the membrane channels in cascade geometry. Using this cascade model, two-step biocatalytic production of l-lactate is demonstrated with concomitant regeneration of soluble nicotinamide adenine dinucleotide (NADH). Both fusion enzymes retained their catalytic activity upon immobilization, suggesting their optimal display on the support surface. The 85% cascading reaction efficiency was achieved at a flow rate that kinetically matches the residence time of the slowest enzyme. In addition, 84% of initial catalytic activity was preserved after 10 days of continuous operation at room temperature. The peptide-directed modular approach described herein is a highly effective strategy to control surface orientation, spatial localization, and loading of multiple enzymes on solid supports. The implications of this work provide insight for the single-step construction of high-power cascadic devices by enabling co-expression, purification, and immobilization of a variety of engineered fusion enzymes on patterned surfaces.
Hardware Synthesis of Explicit Model Predictive Controllers (2007)

The general solution to constrained linear and piecewise linear model predictive control (MPC) has recently been explicitly characterized in terms of piecewise-linear (PWL) state feedback control. This means that a PWL controller can be precomputed using parametric programming, and the exact explicit MPC implementation amounts to the evaluation of a PWL function in the control unit. It has recently been shown that PWL function evaluation can be accelerated by searching a binary tree data structure, leading to highly efficient, accurate, and verifiable software implementation in low-cost embedded control units. In this work, we report hardware synthesis results for this type of PWL control, and show that explicit MPC solutions can be implemented in an application specific integrated circuit (ASIC) with about 20 000 gates, leading to computation times in the microsecond scale. This opens the way for the use of highly advanced control designs such as constrained MPC in small-scale industrial and consumer electronics application areas that are characterized by fast sampling or low cost, including mechatronics, microelectromechanical systems (MEMS), automotive control, power electronics, and acoustics. The main limitation of the approach is that the memory requirements increase rapidly with the problem dimensions
Molecular dynamics studies of aqueous silica nanoparticle dispersions: salt effects on the double layer formation (2015)

The ion distribution around hydroxylated silica nanoparticles (NP-H) dispersed in brine was investigated by fully atomistic molecular dynamics. The NP-H dispersions in aqueous electrolyte media are simulated in solutions of varying salinity (NaCl, CaCl2, and MgCl2), salt concentration (0.06 × 10(-3) to 3.00 × 10(-3) mole fraction [Formula: see text]), and temperature (300 and 350 K) at 1 atm. The NP-H models reproduce the observed experimental concentration of silanol and geminal surface sites, which are responsible for local charge variations on the nanoparticles' surface. Interestingly, under certain salt concentration conditions, the formation of an electrical double layer (DL) around the overall neutral NP-H occurs. The resulting DLs are attenuated with increasing temperature for all evaluated salts. With increasing salt concentration, a sign inversion of the effective charge at the first ion layer is observed, which modifies the electrostatic environment around the nanoparticle. The minimum salt concentration that leads to a DL formation at 300 K is 1.05 × 10(-3), 0.37 × 10(-3), and 0.06 × 10(-3) χs for NaCl, CaCl2, and MgCl2, respectively. The width of the DL decreases sequentially in ionic strength from NaCl to CaCl2 to MgCl2, which is similar to that found for highly charged surfaces. These results are in line with our previous experimental data for negative charged NP-H. All together, these observations suggest an interplay mechanism between the formation and narrowing of electric double layers on the stability of NP dispersions in both neutral and negatively charged NP-H.
Implementierung eines verlustleistungsoptimierten Dezimators für kaskadierte Sigma-Delta Analog-Digital Umsetzer (2005)

Abstract. Dieser Beitrag stellt die Implementierung eines neuartigen Ansatzes einer effizienten Dezimator-Architektur für kaskadierte Sigma-Delta Modulatoren vor. Die Rekombinationslogik kaskadierter Modulatoren und die Korrektur des Verstärkungsfehlers zeitkontinuierlicher (CT) Modulatoren werden in die erste Stufe des Dezimators integriert. Eine entsprechende Filtertopologie wird hergeleitet und auf einem Hardware-Emulator der Firma Mentor Graphics implementiert. Der Vergleich der vorgeschlagenen Struktur mit einer herkömmlichen Implementierung zeigt eine nennenswerte Verbesserung der Effizienz.
Identification, cDNA Cloning, and Targeted Deletion of p70, a Novel, Ubiquitously Expressed SH3 Domain-Containing Protein (2002)

In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an SH3 domain and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. p70 transcripts were found in all tissues examined. Similarly, when an antibody raised against a C-terminal peptide to analyze p70 protein expression was used, all murine tissues examined were found to express p70. To investigate the in vivo role of p70, we generated a p70-deficient mouse strain. Mice lacking p70 are viable, develop normally, and do not display any obvious abnormalities. No differences were detected in various hematological parameters, including bone marrow colony-forming ability, in response to cytokines that utilize Jak2. In addition, no impairment in B- and T-cell development and proliferative ability was detected.
Hybrid kinoform fanout holograms in dichromated gelatin (1991)

The technique for fabricating hybrid holograms, introduced by Bartelt and Case [Appl. Opt. 21, 2886 (1982)], is applied here to record extremely high-efficiency (>90%) space-invariant fanout holograms in dichromated gelatin. The object wave front corresponding to that produced by a kinoform with a continuous-phase profile is derived by appropriate spatial filtering from a binary computer-generated hologram fabricated using electron-beam lithography.
O/W Emulsions with Liquid Crystals as Vehicles of β-Escin (2008)

Abstract Aescin is an important active substance composed of saponin glucosides topical employed in the treatment of inflammatory and edematous conditions. A particular kind of skin care product called α-gel lamellar phases emulsion was developed, containing β-escin. Detailed knowledge of the lamellar phases behavior has been obtained by DSC, rheological measurements and polarized-light optical microscopy. Moreover the effects of lamellar phases on β-escin release profiles have been studied. Keywords: α-gel lamellar phases β-escinrheologythermograms Notes ∗aqueous gel. ∗∗gel emulsion. ∗∗∗α-gel lamellar phases emulsion.
Developing a Microbubble-Based Contrast Agent for Synchrotron In-Line Phase Contrast Imaging (2020)

Identifying a suitable MB for PCI may facilitate future clinical translation.
Spatial Interfaces (2015)

CG&A welcomes Frank Steinicke (University of Hamburg) and Wolfgang Stuerzlinger (Simon Fraser University) as new members to its editorial board and as the new coeditors of the Spatial Interfaces department.
Exercise, GLUT4, and Skeletal Muscle Glucose Uptake (2013)

Glucose is an important fuel for contracting muscle, and normal glucose metabolism is vital for health. Glucose enters the muscle cell via facilitated diffusion through the GLUT4 glucose transporter which translocates from intracellular storage depots to the plasma membrane and T-tubules upon muscle contraction. Here we discuss the current understanding of how exercise-induced muscle glucose uptake is regulated. We briefly discuss the role of glucose supply and metabolism and concentrate on GLUT4 translocation and the molecular signaling that sets this in motion during muscle contractions. Contraction-induced molecular signaling is complex and involves a variety of signaling molecules including AMPK, Ca(2+), and NOS in the proximal part of the signaling cascade as well as GTPases, Rab, and SNARE proteins and cytoskeletal components in the distal part. While acute regulation of muscle glucose uptake relies on GLUT4 translocation, glucose uptake also depends on muscle GLUT4 expression which is increased following exercise. AMPK and CaMKII are key signaling kinases that appear to regulate GLUT4 expression via the HDAC4/5-MEF2 axis and MEF2-GEF interactions resulting in nuclear export of HDAC4/5 in turn leading to histone hyperacetylation on the GLUT4 promoter and increased GLUT4 transcription. Exercise training is the most potent stimulus to increase skeletal muscle GLUT4 expression, an effect that may partly contribute to improved insulin action and glucose disposal and enhanced muscle glycogen storage following exercise training in health and disease.
An event-driven, user-centric, QoS-aware middleware framework for ubiquitous multimedia applications (2001)

In this paper, we present a novel QoS-aware middleware framework, that seamlessly enables ubiquitous delivery of QoS-aware multimedia applications. The QoS management functions are triggered by not only resource fluctuations but also the user's behavior, movement or even light and time. Those activation events are described using asynchronous messages encoded in XML format. Moreover, the QoS-aware middleware services are no longer targeted to applications or devices but users. The QoS management functions are individualized according to the user's preferences and life routines. We have implemented a prototype of our middleware framework as part of the Gaia OS [4], an active space enabling infrastructure. Our experiments with the ubiquitous multimedia streaming application show the soundness of our framework.