gebhart/patrepeval huggingface dataset:
Determination of Cyclic Nucleotide-Dependent Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (1995)

An iterative approach to the a priori determination of the substrate specificity of cAMP- and cGMP-dependent protein kinases (PKA and PKG) by the use of peptide libraries on cellulose paper is described. The starting point of the investigation was an octamer library with the general structure Ac-XXX12XXX, where X represents mixtures of all 20 natural amino acids and 1 and 2 represent individual amino acid residues. The library thus contained all possible 2.56 x 10(10) octamers, divided into 400 sublibraries with defined amino acids 1 and 2 each consisting of 6.4 x 10(7) sequences. After phosphorylation with the kinases in the presence of [gamma-32P]ATP, the sublibrarys Ac-XXXRRXXX and Ac-XXXRKXXX were identified as the best substrates for PKA and PKG, respectively. The second-generation libraries had the structures Ac-XXXRR12X and Ac-XXXRK12X for PKA and PKG and resulted in the most active sequence pools Ac-XXXRRASX and Ac-XXXRKKSX. After delineation of every position in the octameric sequence and extension of the investigation to decameric peptides, the best sequences, Ac-KRAERKASIY and Ac-TQKARKKSNA, were obtained for PKA and PKG, respectively. Promising octameric and decameric peptides were assembled 5 or 10 times each and assayed in order to determine the experimental scatter inherent in the approach. The kinetic data of several octameric and decameric sequences were determined in solution and compared to data for known substrates. The recognition motif of PKA was confirmed by this approach, and a novel substrate sequence for PKG was identified.(ABSTRACT TRUNCATED AT 250 WORDS)
Surface Relaxation Mechanisms in the Morphological Equilibration of Crystal Surfaces (1997)

The phenomenology of surface relaxation below the roughening temperature &lt;i&gt;T&lt;sub&gt;R&lt;/sub&gt;&lt;/i&gt; is shown via kinetic Monte Carlo simulation to exhibit extremely non-classical behavior depending on (1) initial morphology and (2) kinetic rate laws for atom motion. However, within the temperature and size scale of our simulations there are several different mechanisms that operate. The “pinch-off” mechanism is seen to be operative for ripple relaxation employing &lt;i&gt;i&lt;/i&gt;-kinetics. For ripple relaxation employing ∆&lt;i&gt;i&lt;/i&gt;-kinetics, pinch-off is seen at short times. At long times, these particular surfaces spontaneously island, and then reduction of line tension drives relaxation. Reduction of line tension also drives relaxation of dimpled surfaces.
Manual Tracking Performance in Patients with Cerebellar Incoordination: Effects of Mechanical Loading (1990)

Manual tracking performance was studied in five patients with cerebellar incoordination due to unilateral cerebellar hemisphere lesions. The subjects were required to track a target on an oscilloscope screen by moving a cursor controlled by flexion-extension movements of the wrist. In comparison to normal subjects, the cerebellar patients, using their clinically affected arm, demonstrated irregular tracking patterns with inappropriate accelerations and decelerations, numerous high velocity peaks of movement, and an increased time lag between the cursor and the target. The addition of a viscous load provided by feeding back wrist velocity to a torque motor coupled to the apparatus resulted in significant improvement in tracking performance and suppression of the high velocity peaks. Increasing elastic stiffness by feeding back wrist position or inertial load by adding weights to the hand did not improve performance on this task. It is proposed that a hypotonic cerebellar limb behaves like an underdamped mechanical system. The addition of viscous loads helps restore more normal damping during voluntary movements of the arm.
Hybrid ligands with calixarene and thiodigalactoside groups: galectin binding and cytotoxicity (2019)

Galectins have diverse functions and are involved in many biological processes because of their complex intra- and extracellular activities. Selective and potent inhibitors for galectins will be valuable tools to investigate the biological functions of these proteins. Therefore, we describe here the synthesis of galectin inhibitors with a potential "chelate effect". These compounds are designed to bind to two different binding sites on galectins simultaneously. In this paper a series of asymmetric "hybrid" compounds are prepared, which combine two galectin ligands (1) a substituted thiodigalactoside derivative and (2) an antagonist calixarene-based therapeutic agent. NMR spectroscopy was used to evaluate the interactions of these compounds with Galectin-1 and -3. In addition, cellular experiments were conducted to compare the cytotoxic effects of the hybrids with those of a calixarene derivative. While only the thiodigalactoside part of the hybrids showed strong binding, the calixarene part was responsible for observed cytoxoxicity effects, suggesting that the calixarene moiety may also be addressing a non-galectin target.
Premelting of long n-paraffins in chain-extended lamellae. An electron diffraction study (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPremelting of long n-paraffins in chain-extended lamellae. An electron diffraction studyDouglas L. Dorset, Rufina G. Alamo, and Leo MandelkernCite this: Macromolecules 1992, 25, 23, 6284–6288Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November 1992https://pubs.acs.org/doi/10.1021/ma00049a027https://doi.org/10.1021/ma00049a027research-articleACS PublicationsRequest reuse permissionsArticle Views93Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Dual-Wavelength Polarimetric Analysis of the 16 May 2010 Oklahoma City Extreme Hailstorm (2011)

A comparative analysis of a supercell hailstorm using simultaneous observations with S-band and C-band polarimetric radars supported by abundant ground-truth reports is presented in this study. The storm occurred on 16 May 2010 and produced a swath of extremely damaging hail across a large portion of the Oklahoma City, Oklahoma, metro area. Hail sizes over 10 cm in diameter and hail drifts upward of 1.5 m in height were reported. Both S-band (KOUN) and C-band [University of Oklahoma Polarimetric Radar for Innovations in Meteorology and Engineering (OU-PRIME)] polarimetric radars in Norman, Oklahoma, sampled the storm at ranges less than 60 km, so that high-resolution dual-wavelength polarimetric data were obtained. At C band, this analysis mostly presents raw Z and Z DR (due to problems with differential phase resulting from an incorrect censoring threshold in the examined case) while taking into account the possibility of attenuation in the interpretation of these data. Among the issues investigated in the study are the relation of hail size measured at the surface to the polarimetric signatures at both wavelengths, the difference between polarimetric signatures at the two wavelengths of hail aloft and near the surface (where melting hail is mixed with rain), and the three-body scatter spike (TBSS) signature associated with large hail.
The application of high‐speed cinematography for the quantitative analysis of equine locomotion (1980)

Locomotive disorders constitute a serious problem in horse racing which will only be rectified by a better understanding of the causative factors associated with disturbances of gait. This study describes a system for the quantitative analysis of the locomotion of horses at speed. The method is based on high-speed cinematography with a semi-automatic system of analysis of the films. The recordings are made with a 16 mm high-speed camera run at 500 frames per second (fps) and the films are analysed by special film-reading equipment and a mini-computer. The time and linear gait variables are presented in tabular form and the angles and trajectories of the joints and body segments are presented graphically.
Transient swelling, spreading, and drug delivery by a dissolved anti-HIV microbicide-bearing film (2013)

There is a widespread agreement that more effective drug delivery vehicles with more alternatives, as well as better active pharmaceutical ingredients (APIs), must be developed to improve the efficacy of microbicide products. For instance, in tropical regions, films are more appropriate than gels due to better stability of drugs at extremes of moisture and temperature. Here, we apply fundamental fluid mechanical and physicochemical transport theory to help better understand how successful microbicide API delivery depends upon properties of a film and the human reproductive tract environment. Several critical components of successful drug delivery are addressed. Among these are: elastohydrodynamic flow of a dissolved non-Newtonian film; mass transfer due to inhomogeneous dilution of the film by vaginal fluid contacting it along a moving boundary (the locally deforming vaginal epithelial surface); and drug absorption by the epithelium. Local rheological properties of the film are dependent on local volume fraction of the vaginal fluid. We evaluated this experimentally, delineating the way that constitutive parameters of a shear-thinning dissolved film are modified by dilution. To develop the mathematical model, we integrate the Reynolds lubrication equation with a mass conservation equation to model diluting fluid movement across the moving vaginal epithelial surface and into the film. This is a complex physicochemical phenomenon that is not well understood. We explore time- and space-varying boundary flux model based upon osmotic gradients. Results show that the model produces fluxes that are comparable to experimental data. Further experimental characterization of the vaginal wall is required for a more precise set of parameters and a more sophisticated theoretical treatment of epithelium.
Non-psychotropic phytocannabinoid interactions with voltage-gated sodium channels: An update on cannabidiol and cannabigerol (2022)

Phytocannabinoids, found in the plant, <i>Cannabis sativa</i>, are an important class of natural compounds with physiological effects. These compounds can be generally divided into two classes: psychoactive and non-psychoactive. Those which do not impart psychoactivity are assumed to predominantly function <i>via</i> endocannabinoid receptor (CB) -independent pathways and molecular targets, including other receptors and ion channels. Among these targets, the voltage-gated sodium (Nav) channels are particularly interesting due to their well-established role in electrical signalling in the nervous system. The interactions between the main non-psychoactive phytocannabinoid, cannabidiol (CBD), and Nav channels were studied in detail. In addition to CBD, cannabigerol (CBG), is another non-psychoactive molecule implicated as a potential therapeutic for several conditions, including pain <i>via</i> interactions with Nav channels. In this mini review, we provide an update on the interactions of Nav channels with CBD and CBG.
Carbon Dioxide Capturing by Nitrogen-Doping Microporous Carbon (2015)

Nitrogen-containing microprous carbon was successfully synthesized by using phloroglucinol and glutaraldehyde as the carbon source and hydrolyzed silk as the nitrogen source. The porous structures and surface chemical compositions of microporous carbon were analyzed and characterized by nitrogen adsorption isotherms, thermogravimetric analysis, Fourier transform infrared spectrum and scanning electron microscope images. The resultant porous carbons had a microporous structure, and the pore size distribution was 0.7–2.0 nm. Phenolic formaldehyde with silk was pyrolyzed and decomposed to condense a cross-linking structure between 230 and 650 °C. The nitrogen-containing groups from silk decomposition were incorporated into a carbon matrix during the carbonization process. The microporous carbon showed good regeneration performance and high adsorption capacities of CO2 due to its nitrogen-containing groups and developed a micropore structure. Under dynamic conditions, CO2 could be finely separated from a mixture of CO2, N2 and CH4 with microporous carbon, which displayed potential application for CO2 capture.
Holography described by operator algebra (1981)

The operator description of Fourier optics is extended and applied to holography. The existing lens models for ideal holographic processes appear as a self-evident intermediate result; generalization to include apertures, recording-material modulation transfer function, and extended source effects is straightforward. The extended source effect is generally shown to be equivalent to a modification of the actual holographic apertures. The final result is a compact expression for the description of the holographically reconstructed field distribution at an arbitrary plane. A useful, comprehensive list of operator relations is given in two appendixes.
Solving the inverse magnetostatic problem using fictitious magnetic charges (2017)

We present a method for solving the inverse magnetostatic problem using fictitious magnetic charges. The method utilizes the fact that a given magnetic stray field is generated by only one unique charge distribution. Furthermore the usage of fictitious magnetic charges reduces the number of degrees of freedom when solving the inverse problem. We demonstrate two numerical experiments showing that these advantages make the method more efficient and that it has better smoothing properties regarding noise in the input data compared to a previous method using the magnetization.
Importance of extracellular vesicle secretion at the blood–cerebrospinal fluid interface in the pathogenesis of Alzheimer’s disease (2021)

Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathogenesis of Alzheimer's disease (AD). We previously reported that the blood-cerebrospinal fluid (CSF) interface, formed by the choroid plexus epithelial (CPE) cells, releases an increased amount of EVs into the CSF in response to peripheral inflammation. Here, we studied the importance of CP-mediated EV release in AD pathogenesis. We observed increased EV levels in the CSF of young transgenic APP/PS1 mice which correlated with high amyloid beta (Aβ) CSF levels at this age. The intracerebroventricular (icv) injection of Aβ oligomers (AβO) in wild-type mice revealed a significant increase of EVs in the CSF, signifying that the presence of CSF-AβO is sufficient to induce increased EV secretion. Using in vivo, in vitro and ex vivo approaches, we identified the CP as a major source of the CSF-EVs. Interestingly, AβO-induced, CP-derived EVs induced pro-inflammatory effects in mixed cortical cultures. Proteome analysis of these EVs revealed the presence of several pro-inflammatory proteins, including the complement protein C3. Strikingly, inhibition of EV production using GW4869 resulted in protection against acute AβO-induced cognitive decline. Further research into the underlying mechanisms of this EV secretion might open up novel therapeutic strategies to impact the pathogenesis and progression of AD.
Applications for FPGA's on Nanosatellites (2014)

This thesis examines the feasibility of using a Field Programmable Gate Array (FPGA) based design on-board a CubeSat-sized nanosatellite. FPGAs are programmable logic devices that allow for the implementation of custom digital hardware on a single Integrated Circuit (IC). By using these FPGAs in spacecraft, more efficient processing can be done by moving the design onto hardware. A variety of different FPGA-based designs are looked at, including a Watchdog Timer (WDT), a Global Positioning System (GPS) receiver, and a camera interface.
Obesity should not be considered a contraindication to medial Oxford UKA: long-term patient-reported outcomes and implant survival in 1000 knees (2018)

III.
Chemical and isotopic compositions of lavas from the Broken Ridge and Southern Kerguelen Plateau (1995)

Lavas from several major bathymetric highs in the eastern Indian Ocean that are likely to have formed as Early to Middle Cretaceous manifestations of the Kerguelen hotspot are predominantly tholeiitic; so too are glass shards from Eocene to Paleocene volcanic ash layers on Broken Ridge, which are believed to have come from eruptions on the Ninetyeast Ridge. The early dominance of tholeiitic compositions contrasts with the more recent intraplate, alkalic volcanism of the Kerguelen Archipelago. Isotopic and incompatible-element ratios of the plateau lavas are distinct from those of Indian mid-ocean ridge basalts; their Nd, Sr, 207Pb/204Pb and 2078b/204Pb isotopic ratios overlap with but cover a much wider range than measured for more recent oceanic products of the Kerguelen hotspot (including the Ninetyeast Ridge) or, indeed, oceanic lavas from any other hotspot in the world. Samples from the Naturaliste Plateau and ODP Site 738 on the southern tip of the Kerguelen Plateau are particularly noteworthy, with e-Nd(T) = -13 to -7, (87Sr/86Sr)T=0.7090 to 0.7130 and high 207Pb/204Pb relative to 206Pb/204Pb. In addition, the low-e-Nd(T) Naturaliste Plateau samples are elevated in SiO2 (&gt;54 wt%). In contrast to "DUPAL" oceanic islands such as the Kerguelen Archipelago, Pitcairn and Tristan da Cunha, the plateau lavas with extreme isotopic characteristics also have relative depletions in Nb and Ta (e.g., Th/Ta, La Nb &gt; primitive mantle values); the lowest e-Nd(T) and highest Th/Ta and La Nb values occur at sites located closest to rifted continental margins. Accepting a Kerguelen plume origin for the plateau lavas, these characteristics probably reflect the shallow-level incorporation of continental lithosphere in either the head of the early Kerguelen plume or in plume-derived magmas, and suggest that the influence of such material diminished after the period of plateau construction. Contamination of asthenosphere with the type of material affecting Naturaliste Plateau and Site 738 magmatism appears unlikely to be the cause of low-206Pb/204Pb Indian mid-ocean ridge basalts. Finally, because isotopic data for the plateaus do not cluster or form converging arrays in isotope-ratio plots, they provide no evidence for either a quickly evolving, positive ?Nd, relatively high-206Pb/204Pb plume composition, or a plume source dominated by mantle with e-Nd of -3 to ~0.
Polymorphism of Sulindac: Isolation and Characterization of a New Polymorph and Three New Solvates (1997)

The polymorphism of sulindac was investigated. Two polymorphs (I and II) and a new crystalline form (form III) of sulindac were prepared by recrystallization in different solvents. In addition, three new pseudopolymorphs (solvates) from acetone, chloroform, and benzene were obtained, with each containing 1 mol of solvent to 2 mol of sulindac. Different sulindac polymorphs and pseudopolymorphs were characterized by X-ray diffractometry, infrared spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and hot-stage microscopy. The transition behavior of the crystalline forms of sulindac, their melting points, and their enthalpies were investigated by DSC. The melting of form II was observed at 184 degrees C, and form I subsequently recrystallized from this melt. Similarly, form III melts at 145 degrees C and then recrystallizes to form I. We also investigated the influence of the crystallization solvent on sulindac crystal shape.
Particle-in-cell simulation of a radioactive probe in wind (1992)

A two-dimensional particle simulation model was developed to investigate wind effects on charge density measurements under HVDC lines, using a radioactive probe. The particle simulation technique was successful in macroscopically describing the motion of charged particles under the influence of wind, electric field, and diffusion. The simulation results were in good agreement with wind tunnel experiments. The simulation revealed that at higher wind speeds, the charged particles in the ionization region were convected away faster than at lower wind speeds. This reduced the recombination rate at the probe and thereby resulted in a more negative floating potential. At constant wind speed, the floating potential was less negative when the probe emitting surface was in the wake because wind velocity in the wake was much smaller (assumed zero in the simulation.).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Beyond Service Production: Volunteering for Social Innovation (2017)

Building on theories from different fields, we discuss the roles that volunteers can play in the generation, implementation, and diffusion of social innovations. We present a study relying on 26 interviews with volunteer managers, other professionals, volunteers, and one former volunteer in 17 (branches of) third sector organizations in eight European countries. We identify organizational factors that help and hinder volunteer contributions to social innovation. While volunteer contributions to social innovations are encouraged by decentralized organizational structures, systematic “scaling up” of ideas, providing training, and giving a sense of ownership, they are hindered by a reluctant attitude and a lack of resources . This rich, explorative study makes it a fruitful start for further research on the relationship between volunteering and social innovation.
On Criteria for Social Stratification from the Perspective of Social Change (2020)

The criteria for social stratification change along with social change. A study of criteria for social stratification of prominent theories in the field: Marxian theory, Weberian theory, and Bourdieu’s theory of cultural capital, is conducted from the perspective of social change. From the ownership of means of production to multidimensions of wealth, power and prestige, and then to consumption patterns characterized by taste, the changing criteria for social stratification are the result of social differentiation in the process of social change.