gebhart/patrepeval huggingface dataset:
A convenient and practical method for the selective preparation of deuterofluorocarbons (2015)

A detailed study of the development of efficient and practical conditions for the selective synthesis of 1-deuterononafluorobutane from 1-iodononafluorobutane is reported. The optimal conditions involve treatment of the iodo-precursor in D2O at ∼170 °C in the presence of metallic zinc in a sealed Schlenk tube to give a 59% yield of 1-deutero-1,1,2,2,3,3,4,4,4-nonafluorobutane. The same method was applied successfully to two higher homologues to produce 1-deutero-1,1,2,2,3,3,4,4,5,5,5-undecafluoropentane and 1-deutero-1,1,2,2,3,3,4,4,5,5,6,6,6-tridecafluorohexane in yields of 64% and 56%, respectively. Surprisingly, even the non-perfluorinated product 6-deutero-1,1,1,2,2,3,3,4,4-nonafluorohexane could be synthesized in 69% yield with this method.
Percutaneous Cholecystolithotomy: Risks, Benefits, and Long-Term Outcome (1995)

PCCL is moderately effective but, because of the frequency of complications and sludge/stone recurrence, is likely to have only a limited residual role in the era of laparoscopic cholecystectomy.
ChemInform Abstract: Supramolecular Chemistry: The New Architecture? (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
In situ variations in oyster mutagenicity and tissue concentrations of polycyclic aromatic hydrocarbons (1987)

Abstract Variations in oyster mutagenicity were monitored in a series of in situ relocation experiments and compared with tissue concentrations of polycyclic aromatic hydrocarbons (PAHs). Oysters (Crassostrea virginica) were relocated among three rivers in the southern Chesapeake Bay and sampled over a 2-week exposure period. Extracts of oyster tissues were assayed for mutagenic activity by the Salmonella typhimurium microsomal test and analyzed for selected PAHs by gas chromatography. Oysters relocated to the polluted Elizabeth River (Norfolk, VA) from a pristine site (Ware River, VA) accumulated mutagenic activity and PAHs within 3 d following relocation. Mutagenicity increased over a 14-d period while tissue levels of most PAHs stabilized or declined after 3 d. Extracts of native oysters from the Elizabeth River exhibited comparable levels of mutagenic activity as 14-d relocated oysters. In elimination experiments, PAH levels and mutagenic activity of Elizabeth River oysters decreased following relocation to the Ware River and were not detected after 14 d. Regression analyses did not detect a significant association between slopes of mutagenicity dose-responses and tissue levels of PAHs. Marginal associations with numbers of Salmonella revertants per plate were detected in comparisons with sums of PAH tissue levels, and with tissue levels of benzofluoranthene.
Analysis of the effect of inter-bar currents on the performance of polyphase cage-induction motors (2002)

This work uses a methodology based on the 2D-finite elements method (FEM) and on circuit theory (independent currents method) to calculate the inter-bar currents and analyze their effect on the performance of the polyphase cage induction motors. The circuit theory is used to couple the rotor circuit equations to the field equations. The multi-slice technique is used to consider the skew effect.
Glutamine Enhances Immunoregulation of Tumor Growth (1994)

These data indicate that oral Gln supplementation, through support of host Gln stores and glutathione production, may decrease tumor growth by enhancing NK cell activity.
A platform for business-to-business sell-side, private exchanges and marketplaces (2002)

E-commerce, in terms of the monetary value of transactions, is heavily skewed toward business-to-business (B2B) commerce. Numerous B2B sites are already in existence, providing a fairly rich set of functionality for enabling simple or sophisticated trading of goods and services. In the coming years, we expect a strong growth in many kinds of B2B sites for trading, procurement, and sales. In this paper, we present a B2B platform suitable for various kinds of businesses such as large sellers, dealers, distributors, private exchanges, marketplaces, and procurement hubs. We present business scenarios, describe our design criteria, and present the architecture for the B2B platform. We discuss two major IBM offerings within the WebSphere® Commerce Suite product— WebSphere Commerce Suite, Marketplace Edition (MPE) and WebSphere Commerce Business Edition (BE)—that are based on this platform. We also present some experiences with and deployment of MPE for private exchanges and marketplace s.
Research activity among European hand surgery residents (2019)

During residency, most hand surgeons may be encouraged to participate in clinical or basic science research projects. The research committee of the Federation of European Societies for Surgery of the Hand (FESSH) is dedicated to support research activities, especially of young surgeons, including residents. In 2018, we initiated a survey among European hand surgeons to assess hurdles that limit research activity during hand surgery training and to explore if FESSH can take a more active role in promoting research activities.
Human suppressor T cell clones lack CD28 (1990)

Previously we showed that certain T cell lines and clones from a lepromatous leprosy patient displayed a dose-dependent suppression of the proliferation of autologous T cells to Mycobacterium leprae (M. leprae) but not mitogen or an unrelated antigen. The latter cells were also cloned and did not display this suppressive activity, were CD4+ and proliferated vigorously to M. leprae presented by autologous HLA-DR molecules. We shall refer to these cells as T helper (Th) cells. Most of the suppressive T cell clones (Ts) were also CD4+ and also proliferated to M. leprae presented by HLA-DR, but much less strongly than Th cells. In this study we report on our search for (a) the mechanism of this apparently antigen-specific suppression by T cells, and (b) a possible phenotypic difference between Th and Ts clones. The two main conclusions are that Ts clones possess a lytic machinery, but that M. leprae-specific suppression and cytotoxicity can be clearly dissociated, and that the only phenotypic difference between Th and Ts is the presence of the CD28 marker on Th and its absence on Ts clones.
Repeated cultivation: non-cell disruption extraction of astaxanthin for Haematococcus pluvialis (2016)

The operation of cell disruption is indispensable but cost much in microalgae industry. To be simplified, two different reaction mechanisms await in the cell to respond to moderated or stressed environment. The physical and chemical changes of enzyme and turgor pressure of cell in this conversion play an important role in the enhancement of biomass and metabolites. Repeated turgor pressure (based on the structure and mechanics of cell wall) and converted enzyme system (based on photosynthesis) were used to loosen cell wall and then repeated cultivation of Haematococcus pluvialis for astaxanthin extraction was proposed. There was no significant difference of extraction yield between the broken cell (94.75 ± 3.13%) and non-broken cell (92.32 ± 3.24%) treated by the repeated cultivation. Meanwhile, fed-batch culture according to the relationship among pH and nutrient concentration was used to enhance the biomass of Haematococcus pluvialis with the dry cell weight of 1.63 ± 0.07 g/L.
Performance analysis of time diversity combining with frequency offset for cellular IoT (2017)

In the growing context of Machine Type Communications (MTC), synthesized by the concept of Internet of Things (IoT), the need of an increased coverage pushes the industrial companies to develop efficient transmission techniques. Especially, the 3rd Generation Partnership Project (3GPP) already standardized evolutions of the 2G and 4G networks to include IoT features. These new standards share the common trait of using blind repetitions to improve the legacy coverage of the system, renewing interest for diversity combining. The present paper focuses on the IQ combiner approach which works on complex baseband samples without any previous phase synchronization. Our study evaluates the impact of the frequency offset, resulting of an imperfect frequency synchronization, on the average Signal to Noise Ratio (SNR) of IQ-combined frames. We derive new closed-forms: a first one for the statistical distribution of the residual phase angle between two consecutive symbols after imperfect frequency demodulation, a second one for the average SNR of the combined frame. These derivations allow us to highlight and quantify the negative impact of an inaccurate frequency synchronization in the context of IQ combining strategies.
Kinetic Analysis and X-ray Structure of Haloalkane Dehalogenase with a Modified Halide-Binding Site (1998)

Haloalkane dehalogenase (DhlA) catalyzes the hydrolysis of haloalkanes via an alkyl-enzyme intermediate. Trp175 forms a halogen/halide-binding site in the active-site cavity together with Trp125. To get more insight in the role of Trp175 in DhlA, we mutated residue 175 and explored the kinetics and X-ray structure of the Trp175Tyr enzyme. The mutagenesis study indicated that an aromatic residue at position 175 is important for the catalytic performance of DhlA. Pre-steady-state kinetic analysis of Trp175Tyr-DhlA showed that the observed 6-fold increase of the Km for 1,2-dibromoethane (DBE) results from reduced rates of both DBE binding and cleavage of the carbon-bromine bond. Furthermore, the enzyme isomerization preceding bromide release became 4-fold faster in the mutant enzyme. As a result, the rate of hydrolysis of the alkyl-enzyme intermediate became the main determinant of the kcat for DBE, which was 2-fold higher than the wild-type kcat. The X-ray structure of the mutant enzyme at pH 6 showed that the backbone structure of the enzyme remains intact and that the tyrosine side chain lies in the same plane as Trp175 in the wild-type enzyme. The Clalpha-stabilizing aromatic rings of Tyr175 and Trp125 are 0.7 A further apart and due to the smaller size of the mutated residue, the volume of the cavity has increased by one-fifth. X-ray structures of mutant and wild-type enzyme at pH 5 demonstrated that the Tyr175 side chain rotated away upon binding of an acetic acid molecule, leaving one of its oxygen atoms hydrogen bonded to the indole nitrogen of Trp125 only. These structural changes indicate a weakened interaction between residue 175 and the halogen atom or halide ion in the active site and help to explain the kinetic changes induced by the Trp175Tyr mutation.
Prognostic significance of vascular endothelial growth factor polymorphisms in colorectal cancer patients (2017)

The CT and CC genotypes of the <i>VEGF-A</i> -1498C>T and the CC genotype of the <i>VEGF-A</i> -634C>G polymorphisms are prognostic factors of survival in Brazilians patients with sporadic colorectal carcinoma.
Monoclonal antibodies against creatine kinase isoenzymes (1985)

Conference Article| February 01 1985 Monoclonal antibodies against creatine kinase isoenzymes ANTONY P. JACKSON; ANTONY P. JACKSON 1Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, U.K. Search for other works by this author on: This Site PubMed Google Scholar KENNETH SIDDLE; KENNETH SIDDLE 1Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, U.K. Search for other works by this author on: This Site PubMed Google Scholar R. J. THOMPSON R. J. THOMPSON 1Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1985) 13 (1): 99–100. https://doi.org/10.1042/bst0130099 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation ANTONY P. JACKSON, KENNETH SIDDLE, R. J. THOMPSON; Monoclonal antibodies against creatine kinase isoenzymes. Biochem Soc Trans 1 February 1985; 13 (1): 99–100. doi: https://doi.org/10.1042/bst0130099 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1985 Biochemical Society1985 Article PDF first page preview Close Modal You do not currently have access to this content.
Empirical acousto-optic sonar performance versus water surface condition (2002)

Developments in underwater sound detection by laser sensors probing the air-water surface are presented and discussed. Experimental results are presented that demonstrate laser sensor performance on detecting underwater sound subject to various water surface conditions. The results are used for evaluating current laser sensor capabilities and lay the foundation for future hardware design.
Structure and morphology of isotactic polypropylene functionalized by electron beam irradiation (2000)

The structure and morphology of isotactic polypropylene (iPP), functionalized by electron beam irradiation at room temperature in air, are investigated by elementary analysis, FT-infrared (FTIR) spectroscopy, electron spectroscopy for chemical analysis (ESCA), polariscope, and static contact angle. Elementary analysis reveals that the element oxygen has been introduced onto iPP chains after electron beam irradiation. In addition, as shown from FTIR spectra, oxygen-containing groups, such as carbonyl, carboxyl, and ether groups, are introduced onto iPP molecular chains. The dependence of oxygenation extent (as measured by O1S/C1S value of ESCA spectra) on electron beam dose is obtained. Under polariscope, it can be observed that the dominant alpha phase appears to become more enhanced, and there is no crystalline phase transition. The static contact angle of iPP decreases with increasing dose. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 76: 75–82, 2000
Supplementary Tables 1-2 from Quantitative and Functional Alterations of Plasmacytoid Dendritic Cells Contribute to Immune Tolerance in Ovarian Cancer (2023)

Supplementary Tables 1-2 from Quantitative and Functional Alterations of Plasmacytoid Dendritic Cells Contribute to Immune Tolerance in Ovarian Cancer
The Role of Dimethyl Sulfoxide (DMSO) in Gene Expression Modulation and Glycosaminoglycan Metabolism in Lysosomal Storage Disorders on an Example of Mucopolysaccharidosis (2019)

Obstacles to effective therapies for mucopolysaccharidoses (MPSs) determine the need for continuous studies in order to enhance therapeutic strategies. Dimethyl sulfoxide (DMSO) is frequently utilised as a solvent in biological studies, and as a vehicle for drug therapy and the in vivo administration of water-insoluble substances. In the light of the uncertainty on the mechanisms of DMSO impact on metabolism of glycosaminoglycans (GAGs) pathologically accumulated in MPSs, in this work, we made an attempt to investigate and resolve the question of the nature of GAG level modulation by DMSO, the isoflavone genistein solvent employed previously by our group in MPS treatment. In this work, we first found the cytotoxic effect of DMSO on human fibroblasts at concentrations above 3%. Also, our results displayed the potential role of DMSO in the regulation of biological processes at the transcriptional level, then demonstrated a moderate impact of the solvent on GAG synthesis. Interestingly, alterations of lysosomal ultrastructure upon DMSO treatment were visible. As there is growing evidence in the literature that DMSO can affect cellular pathways leading to numerous changes, it is important to expand our knowledge concerning this issue.
leu operon of Salmonella typhimurium is controlled by an attenuation mechanism. (1979)

The nucleotide sequence of the control region of the leu operon of Salmonella typhimurium was determined. A prominent feature of this region is a signal for termination of transcription. In vitro, transcription does terminate at this site, yielding a leader RNA of about 160 nucleotides as a major product. This leader RNA is potentially translatable into a peptide containing 28 amino acids, 4 of which are adjacent leucine residues. Several regions of base complementarity exist within the leader, positioned such that pairing of one region precludes pairing of another. The position of the four leucine codons relative to two regions of base complementarity suggest a model for the regulation of the leu operon similar to that proposed by Yanofsky and coworkers for the trp operon. In addition, a third region of base complementarity was identified which, when incorporated into the model, explains why premature termination is the usual outcome when transcription is initiated in vitro by purified RNA polymerase.
Sweet plasmonics: Sucrose macrocrystals of metal nanoparticles (2014)

The realization of plasmonic structures generally necessitates expensive fabrication techniques, such as electron beam and focused ion beam lithography, allowing for the top-down fabrication of low-dimensional structures. Another approach to make plasmonic structures in a bottom-up fashion is colloidal synthesis, which is convenient for liquid-state applications or very thin solid films where aggregation problems are an important challenge. The architectures prepared using these methods are typically not robust enough for easy handling and convenient integration. Therefore, developing a new plasmonic robust platform having large-scale dimensions without adversely affecting the plasmonic features is in high demand. As a solution, here we present a new plasmonic composite structure consisting of gold nanoparticles (Au NPs) incorporated into sucrose macrocrystals on a large scale, while preserving the plasmonic nature of the Au NPs and providing robustness in handling at the same time. As a proof of concept demonstration, we present the fluorescence enhancement of green CdTe quantum dots (QDs) via plasmonic coupling with these Au NPs in the sucrose crystals. The obtained composite material exhibits centimeter scale dimensions and the resulting quantum efficiency (QE) is enhanced via the interplay between the Au NPs and CdTe QDs by 58% (from 24% to 38%). Moreover, a shortening in the photoluminescence lifetime from 11.0 to 7.40 ns, which corresponds to a field enhancement factor of 2.4, is observed upon the introduction of Au NPs into the QD incorporated macrocrystals. These results suggest that such “sweet” plasmonic crystals are promising for large-scale robust platforms to embed plasmonic nanoparticles.
Assignment of the human PAX4 gene to chromosome band 7q32 by fluorescence in situ hybridization (1994)

Of the nine known members of a human paired box-containing gene family (Pax), only PAX4 has not been precisely localized. We screened a cosmid library of human genomic DNA using polymerase chain reaction products for PAX4 as a probe and isolated three positive cosmid clones. Sequence analysis revealed that at least two of them had exon-like sequences and showed extensive homology to Pax-4 in the mouse. These two cosmid clones were mapped to human chromosome band 7q32 by fluorescence in situ hybridization.
Ex-vivo gene therapy of the liver: new approach to tackle hepatitis C infection and recurrence after liver transplantation. (2005)

Henry, SD; van der Wegen, PG; Metselaar, HJ; Bartenschlager, R; Tilanus, HW; Scholte, BJ; van der Laan, LJW Author Information
Gene transcription analysis during interaction between potato and Ralstonia solanacearum (2010)

Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs.
Domestic animal genetics (2021)

Domestication of wild animal and plant species may be considered the largest genetic experiment in human history. Human selection for specific traits and targeted breeding over hundreds or thousands of generations has shaped very special populations that provide unique opportunities for research. Domestic animal genetics contributes to technological advances facilitating a more sustainable, environment and climate friendly food production that respects changing societal expectations related to ethical and animal welfare issues. Domestic animal genetics also enables fundamental insights into gene functions and provides animal models for biomedical research. Under this theme, we present a selection of PLOS Genetics publications that exemplify recent progress in domestic animal genetics. Rather than grouping by species, we assigned the publications to 5 thematically related topics, which we will briefly introduce in the following sections.
Recent Advances in Understanding Immunity Against Brucellosis: Application for Vaccine Development (2010)

Brucellosis is an important zoonotic disease of nearly worldwide distribution. This pathogen causes abortion in cattle and undulant fever, arthritis, endocarditis and meningitis in human. The immune response against B. abortus involves innate and adaptive immunity involving antigen-presenting cells, NK cells and CD4 + and CD8 + T cells. IFN-is a crucial immune component that results from Brucella recognition by host immune receptors such as Toll-like receptors (TLRs) that lead to IL-12 production. Although great efforts to elucidate immunity against Brucella have been employed, the subset of cells and factors involved in host immune response remains not completely understood. Our group and others have been working in an attempt to understand the mechanisms involved in innate responses to Brucella. Understanding the requirements for immune protection can help the design of alternative vaccines that would avoid the drawbacks of currently available vaccines to Brucella. This review discusses recent studies in host immunity to Brucella and new approaches for vaccine development.