gebhart/patrepeval huggingface dataset:
Effects of Recombinant Tissue Plasminogen Activator After Intraluminal Thread Occlusion in Mice: Role of Hemodynamic Alterations (2001)

We provide evidence that rtPA provokes complex hemodynamic alterations in the ischemic brain tissue, which include an initial hyperperfusion and a more delayed hypoperfusion response. Changes are most pronounced in the periphery of the ischemic infarct, where regional blood flow drops below critical thresholds of tissue viability. Our observations suggest that changes of perfusion may at least partly explain the rtPA-induced increase of infarct size, which has previously been reported and which we also confirmed in the present experiments. Notably, both the secondary hypoperfusion and increase of infarct volume were abolished when rtPA-treated animals received additional heparin infusions. This finding suggests that a secondary hypercoagulability may compromise brain perfusion after rtPA delivery. Accordingly, early treatment with heparin might help to prevent the rtPA-induced changes.
Development and Validation of a Novel Food-Based Global Diet Quality Score (GDQS) (2021)

The simplicity of the GDQS and its ability to capture both nutrient adequacy and diet-related NCD risk render it a promising candidate for global monitoring platforms. Research is warranted to validate methods to operationalize GDQS assessment in population surveys, including a novel application-based 24-h recall system developed as part of this project.
Poly peak parser: Method and software for identification of unknown indels using sanger sequencing of polymerase chain reaction products (2014)

The method and algorithm described here facilitate rapid identification and sequence characterization of heterozygous mutant carriers generated by genome editing. Although designed for screening F1 individuals, this tool can also be used to identify heterozygous indels in many contexts.
Acquired obesity and poor physical fitness impair expression of genes of mitochondrial oxidative phosphorylation in monozygotic twins discordant for obesity (2008)

Defects in expression of genes of oxidative phosphorylation in mitochondria have been suggested to be a key pathophysiological feature in familial insulin resistance. We examined whether such defects can arise from lifestyle-related factors alone. Fourteen obesity-discordant (BMI difference 5.2 +/- 1.8 kg/m(2)) and 10 concordant (1.0 +/- 0.7 kg/m(2)) monozygotic (MZ) twin pairs aged 24-27 yr were identified among 658 MZ pairs in the population-based FinnTwin16 study. Whole body insulin sensitivity was measured using the euglycemic hyperinsulinemic clamp technique. Transcript profiles of mitochondrial genes were compared using microarray data of fat biopsies from discordant twins. Body composition of twins was determined using DEXA and maximal oxygen uptake (Vo(2max)) and working capacity (W(max)) using a bicycle ergometer exercise test with gas exchange analysis. The obese cotwins had lower insulin sensitivity than their nonobese counterparts (M value 6.1 +/- 2.0 vs. 9.2 +/- 3.2 mg x kg LBM(-1) x min(-1), P < 0.01). Transcript levels of genes involved in the oxidative phosphorylation pathway (GO:0006119) in adipose tissue were lower (P < 0.05) in the obese compared with the nonobese cotwins. The obese cotwins were also less fit, as measured by Vo(2max) (50.6 +/- 6.5 vs. 54.2 +/- 6.4 ml x kg LBM(-1) x min(-1), for obese vs. nonobese, P < 0.05), W(max) (3.9 +/- 0.5 vs. 4.4 +/- 0.7 W/kg LBM, P < 0.01) and also less active, by the Baecke leisure time physical activity index (2.8 +/- 0.5 vs. 3.3 +/- 0.6, P < 0.01). This implies that acquired poor physical fitness is associated with defective expression of the oxidative pathway components in adipose tissue mitochondria.
Ghent University-Department of Textiles: annual report 2013 (2013)

Several students from abroad stayed at the department to follow lectures and to perform
Transgenic expression of human matrix metalloproteinase-9 augments monocrotaline-induced pulmonary arterial hypertension in mice (2010)

Our study demonstrated that the MCT-induced PAH in mouse is a reproducible and potentially valuable animal model for the human disease. Our results further demonstrated that MMP-9 plays a significant role in the pathogenesis of PAH and effective blocking of MMP-9 could provide an option in the therapeutic intervention of human PAH.
Statin administration before percutaneous coronary intervention: impact on periprocedural myocardial infarction (2004)

Pre-procedural statin therapy reduces the incidence of large non-Q-wave myocardial infarction after PCI.
Comparative Clinicopathological Responses of Striped Bass and Palmetto Bass to Acute Stress (1999)

Selected clinicopathological features were compared between resting striped bass Morone saxatilis and palmetto bass (striped bass female × white bass M. chrysops male) and fish subjected to an acute (2-h-long) confinement stress. The taxa differed significantly in resting plasma lysozyme activities and leukocyte responses to mitogen stimulation. Confined fish of both taxa showed similar elevations in plasma osmolality, potassium, anion gap, creatinine, and glucose, suggesting a shock response. However, striped bass displayed slightly more severe perturbations, including elevated albumin and total protein, that indicated hemoconcentration. At least some of the intertaxon differences may have been associated with the greater ability of palmetto bass to adapt to culture conditions.
Release of Colony Stimulating Factor (CSF) by Non-Endotoxic Breakdown Products of Bacterial Lipopolysaccharides (1974)

S. Minnesota 1114 lipopolysaccharide (LPS) has been partially hydrolyzed into a “Lipid A” precipitate and a water-soluble, endo-toxin-free fraction (PS). The latter fraction was as active in CSF as the intact LPS. A rough mutant of, S. Minnesota, the heptoseless R595 strain, synthesizes the lipid moiety of LPS only with KDO attached to it. This glycolipid and the above “Lipid A” preparations were significantly less active than the LPS or PS preparations.
The potential role of rapamycin in pediatric transplantation as observed from adult studies (1999)

Although pediatric patient and renal graft survival rates have shown marked improvements during the past decade, the persistent toxicities of immunosuppressive drugs and chronic allograft attrition remain major obstacles in transplant therapy. Results in adult patients suggest that complete steroid withdrawal is possible in the majority of recipients under treatment with a cyclosporin A-rapamycin (CsA RAPA) regimen. Furthermore, preliminary studies suggest that a marked reduction in the dose of CsA may be possible under the umbrella of RAPA coverage. The gain in immunosuppressive efficacy afforded by RAPA has not only been obtained without an increased morbidity owing to infectious or neoplastic causes, but also with the potential for reducing the incidence and/or progression of chronic rejection.
Room temperature radioassay for B12 with oyster toadfish (Opsanus tau) serum as binder. (1980)

Abstract We describe a vitamin B12 radioassay with oyster toadfish (Opsanus tau) serum in which endogenous serum B12 is released from its protein complexes at room temperature with NaOH at pH 13.6. The radioassay is reproducible (CV 4.9% within assay, 8.4% between assays) and sensitive (10 ng/L) and requires minimum manipulations. Assay accuracy is unaffected by serum protein.
Mechanisms of copper removal during chemical mechanical polishing (1995)

Schemes using chemical mechanical polishing of copper have been proposed for the patterning of interconnections in copper multilevel metallization. In this article, the phenomena involved in the removal of copper during copper chemical mechanical polishing are investigated. The concentration of chemical etchant in the polish slurry is varied to investigate the chemical component, and the applied pressure is varied to investigate the mechanical component. Two slurries, an ammonium hydroxide plus ferricyanide slurry and a nitric acid slurry, are used to polish both copper and Cu2O thin films. Removal of copper or Cu2O is hypothesized to be a result of mechanical abrasion, while the role of the chemical etchant is to dissolve the material abraded from the surface rather than to etch the material directly from the surface.
Hermenêutica jurídica: princípios e aplicação (2012)

Tomando por base a Hermeneutica como teoria ou filosofia da interpretacao, expressa tambem na arte de interpretar, o presente artigo tem por escopo apresentar as formas diferentes de se compreender, utilizados pelo homem para alcancar os significados. Assim, diante dos principios maiores que informam o papel do interprete, conclui que todos os processos e meios para interpretacao poderao ser utilizados, na busca do sentido e o alcance das expressoes do direito.
Protective Thin Film Coatings by Plasma Polymerisation (1986)

The paper describes the plasma polymerisation process for depositing ultrathin films. Such films, deposited from monomers containing hydrophobic elements such as hexafluorobenzene and hexamethyldisiloxane, showed excellent water vapour barrier properties, due to dense, highly crosslinked and rigid structures of the films. The composition and structure of the plasma polymerised films have been elucidated by ESCA and infra-red spectroscopy.
A MUSIC-Based Algorithm for Blind User Identification in Multiuser DS-CDMA (2005)

A blind scheme based on multiple-signal classification (MUSIC) algorithm for user identification in a synchronous multiuser code-division multiple-access (CDMA) system is suggested. The scheme is blind in the sense that it does not require prior knowledge of the spreading codes. Spreading codes and users' power are acquired by the scheme. Eigenvalue decomposition (EVD) is performed on the received signal, and then all the valid possible signature sequences are projected onto the subspaces. However, as a result of this process, some false solutions are also produced and the ambiguity seems unresolvable. Our approach is to apply a transformation derived from the results of the subspace decomposition on the received signal and then to inspect their statistics. It is shown that the second-order statistics of the transformed signal provides a reliable means for removing the false solutions.
Viral biogeography revealed by signatures in Sulfolobus islandicus genomes (2009)

Viruses are a driving force of microbial evolution. Despite their importance, the evolutionary dynamics that shape diversity in viral populations are not well understood. One of the primary factors that define viral population structure is coevolution with microbial hosts. Experimental models predict that the trajectory of coevolution will be determined by the relative migration rates of viruses and their hosts; however, there are no natural microbial systems in which both have been examined. The biogeographic distribution of viruses that infect Sulfolobus islandicus is investigated using genome comparisons among four newly identified, integrated, Sulfolobus spindle-shaped viruses and previously sequenced viral strains. Core gene sequences show a biogeographic distribution where viral genomes are specifically associated with each local population. In addition, signatures of host-virus interactions recorded in the sequence-specific CRISPR (clustered regularly interspaced short palindromic repeats) system show that hosts have interacted with viral communities that are more closely related to local viral strains than to foreign ones. Together, both proviral and CRISPR sequences show a clear biogeographic structure for Sulfolobus viral populations. Our findings demonstrate that virus-microbe coevolution must be examined in a spatially explicit framework. The combination of host and virus biogeography suggests a model for viral diversification driven by host immunity and local adaptation.
Ultrafast Modulation of the Plasma Frequency of Vertically Aligned Indium Tin Oxide Rods (2014)

Light-matter interaction at the nanoscale is of particular interest for future photonic integrated circuits and devices with applications ranging from communication to sensing and imaging. In this Letter a combination of transient absorption (TA) and the use of third harmonic generation as a probe (THG-probe) has been adopted to investigate the response of the localized surface plasmon resonances (LSPRs) of vertically aligned indium tin oxide rods (ITORs) upon ultraviolet light (UV) excitation. TA experiments, which are sensitive to the extinction of the LSPR, show a fluence-dependent increase in the frequency and intensity of the LSPR. The THG-probe experiments show a fluence-dependent decrease of the LSPR-enhanced local electric field intensity within the rod, consistent with a shift of the LSPR to higher frequency. The kinetics from both TA and THG-probe experiments are found to be independent of the fluence of the pump. These results indicate that UV excitation modulates the plasma frequency of ITO on the ultrafast time scale by the injection of electrons into, and their subsequent decay from, the conduction band of the rods. Increases to the electron concentration in the conduction band of ∼13% were achieved in these experiments. Computer simulation and modeling have been used throughout the investigation to guide the design of the experiments and to map the electric field distribution around the rods for interpreting far-field measurement results.
ChemInform Abstract: Vitamin B12. How the Problem of Its Biosynthesis was Solved (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Enzymatic Reaction of Silent Substrates:  Kinetic Theory and Application to the Serine Protease Chymotrypsin (2003)

Investigating the selectivity that an enzyme expresses toward its substrates can be technically challenging if reaction of these substrates is not accompanied by a conveniently monitored change in some physicochemical property. In this paper, we describe a simple method for determining steady-state kinetic parameters for enzymatic turnover of such "silent" substrates. According to this method, silent substrate S is allowed to compete for enzymic reaction with signal-generating substrate S*, whose conversion to product can be conveniently monitored. Full reaction progress curves are collected under conditions of [S*](o) << K(m)* and [S](o) >or= 3K(m). Progress curves collected under these conditions are characterized by an initial lag phase of duration tau that is followed by the pseudo-first-order reaction of S. Steady-state kinetic parameters for the silent substrate can be obtained by one of two methods. One method combines least-squares fitting with numerical integration of appropriate rate equations to analyze the progress curves, while the other method relies on direct graphical analysis in which K(m) is the value of [S](o) that reduces the control velocity by a factor of 2 and V(max) is shown to simply equal the ratio [S](o)/tau. We use these methods to analyze the alpha-chymotrypsin-catalyzed hydrolysis of silent substrate Suc-Ala-Phe-AlaNH(2) with signal generator Suc-Ala-Phe-pNA. From the curve-fitting method, k(c) = 0.9 +/- 0.2 s(-1) and K(m) = 0.4 +/- 0.1 mM, while by direct graphical analysis, k(c) = 1.1 +/- 0.1 s(-1) and K(m) = 0.51 +/- 0.03 mM. As validation of this new method, we show agreement of these values with those determined independently by HPLC analysis of the hydrolysis of Suc-Ala-Phe-AlaNH(2) by alpha-CT, where k(c) = 1.1 +/- 0.1 s(-1) and K(m) = 0.5 +/- 0.1 mM.
Hybrid Molecular Systems Containing Tetrathiafulvalene and Iron‐Alkynyl Electrophores: Five‐Component Functional Molecules Obtained from CH Bond Activation (2013)

Treatment of [Cp*(dppe)Fe-C≡C-TTFMe3] (1) with Ag[PF6] (3 equiv) in DMF provides the binuclear complex [Cp*(dppe)Fe=C=C=TTFMe2 =CH-CH=TTFMe2 =C=C=Fe(dppe)Cp*][PF6]2 (2[PF6 ]2) isolated as a deep-blue powder in 69 % yield. EPR monitoring of the reaction and comparison of the experimental and calculated EPR spectra allowed the identification of the radical salt [Cp*(dppe)Fe=C=C=TTFMe2 =CH][PF6]2 ([1-CH][PF6]) an intermediate of the reaction, which results from the activation of the methyl group attached in vicinal position with respect to the alkynyl-iron on the TTF ligand by the triple oxidation of 1 leading to its deprotonation by the solvent. The dimerization of [1-CH][PF6] through carbon-carbon bond formation provides 2[PF6]2. The cyclic voltammetry (CV) experiments show that 2[PF6]2 is subject to two sequential well-reversible one-electron reductions yielding the complexes 2[PF6] and 2. The CV also shows that further oxidation of 2[PF6]2 generates 2[PF6]n (n=3-6) at the electrode. Treatment of 2[PF6]2 with KOtBu provides 2[PF6] and 2 as stable powders. The salts 2[PF6] and 2[PF6]2 were characterized by XRD. The electronic structures of 2(n+) (n=0-2) were computed. The new complexes were also characterized by NMR, IR, Mössbauer, EPR, UV/Vis and NIR spectroscopies. The data show that the three complexes 2[PF6]n are iron(II) derivatives in the ground state. In the solid state, the dication 2(2+) is diamagnetic and has a bis(allenylidene-iron) structure with one positive charge on each iron building block. In solution, as a result of the thermal motion of the metal-carbon backbone, the triplet excited state becomes thermally accessible and equilibrium takes place between singlet and triplet states. In 2[PF6], the charge and the spin are both symmetrically distributed on the carbon bridge and only moderately on the iron and TTFMe2 electroactive centers.
ChemInform Abstract: Reaction of Alkyl Hypochlorites and Xenon Difluoride with Cyclohexene. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Etching of quartz crystal spheres (2002)

Natural quartz crystal spheres were etched in various etchants at room temperature and at 80/spl deg/C. Ten 12-mm-diameter polished natural quartz spheres were placed into each of several etchants, including 40% HF:60% NH/sub 4/F, saturated NH/sub 4/F-HF and lattice etch, at each of two nominal temperatures: 20/spl deg/C and 80/spl deg/C. The resulting quartz pieces were scanning electron micrographed normal to the resulting major plane and at right angles to the plane for the purpose of illustrating the anisotropy of the etch rate of quartz as a function of etchant and etchant temperature. The micrographs are presented and evaluated. All the etchants exhibit distinctly different figures at room temperature versus 80/spl deg/C. This is consistent with earlier reports that crystal blanks etched at room temperature are generally "smoother" (i.e., different) than similar blanks etched in the same etchant at elevated temperatures.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Further Remarks on Electron Beam Pumping of Laser Materials (1966)

This article demonstrates that recently completed studies on the energy dissipation of kilovolt electron beams in solids provide readily applicable methods for assessing the situation in electron beam pumped lasers.
APPARATUS FOR TEACHING PHYSICS: B-H Curve Tracer for Cylindrical Samples (1970)

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Interrelationships of Transcriptional Machinery with Nuclear Architecture (1999)

There is increasing evidence for functional linkages between nuclear architecture and the regulation of gene expression. The nuclear matrix provides a paradigm for involvement of nuclear morphology in assembly of the biochemical machinery for physiological control of transcription. Mechanisms are being experimentally defined that direct regulatory factors to subnuclear sites that support gene expression. Consequently, we are gaining insight into the rules that govern transcriptional control within the three-dimensional context of nuclear organization.
Trapping mechanism on oxygen-terminated diamond surfaces (2006)

Mechanisms of the hole trap and detrap on the oxygen-terminated diamond surfaces measured by diamond in-plane-gated field-effect transistors (FETs) have been investigated. Reproducible hysteresis characteristics are observed in the IDS-VGS characteristics of the diamond in-plane-gated FETs. They are caused by carrier trapping in the oxidized diamond surface and detrapping under a light irradiation, the wavelength of which affects the hysteresis width. Carriers are trapped by continuous surface states deeper than 2.0eV from the valence band maximum in the oxidized diamond surface, where the position of the highest occupied level (Fermi level) is located between 2.0 and 2.4eV.
Distinct plasma gradients of microRNA‐204 in the pulmonary circulation of patients suffering from WHO Groups I and II pulmonary hypertension (2019)

Pulmonary hypertension (PH), a heterogeneous vascular disease, consists of subtypes with overlapping clinical phenotypes. MicroRNAs, small non-coding RNAs that negatively regulate gene expression, have emerged as regulators of PH pathogenesis. The muscle-specific micro RNA (miR)-204 is known to be depleted in diseased pulmonary artery smooth muscle cells (PASMCs), furthering proliferation and promoting PH. Alterations of circulating plasma miR-204 across the trans-pulmonary vascular bed might provide mechanistic insights into the observed intracellular depletion and may help distinguish PH subtypes. MiR-204 levels were quantified at sequential pulmonary vasculature sites in 91 patients with World Health Organization (WHO) Group I pulmonary arterial hypertension (PAH) (n = 47), Group II PH (n = 22), or no PH (n = 22). Blood from the right atrium/superior vena cava, pulmonary artery, and pulmonary capillary wedge was collected. Peripheral blood mononuclear cells (PBMCs) were isolated (n = 5/group). Excretion of miR-204 by PAH-PASMCs was also quantified in vitro. In Group I patients only, miR-204 concentration increased sequentially along the pulmonary vasculature (log fold-change slope = 0.22 [95% CI = 0.06-0.37], P = 0.008). PBMCs revealed insignificant miR-204 variations among PH groups ( P = 0.12). Cultured PAH-PAMSCs displayed a decrease of intracellular miR-204 ( P = 0.0004), and a converse increase of extracellular miR-204 ( P = 0.0018) versus control. The stepwise elevation of circulating miR-204 across the pulmonary vasculature in Group I, but not Group II, PH indicates differences in muscle-specific pathobiology between subtypes. Considering the known importance of miR-204 in PH, these findings may suggest pathologic excretion of miR-204 in Group I PAH by PASMCs, thereby accounting for decreased intracellular miR-204 concentration.
Depth-enhanced integral floating imaging system with variable image planes using polymer-dispersed liquid-crystal films (2008)

A depth-enhanced three-dimensional integral floating imaging system with variable imaging planes is proposed. Polymer-dispersed liquid-crystal films are used for implementing the variable image planes. The proposed method is described and experiment results are provided.