gebhart/patrepeval huggingface dataset:
Preparation of monovalent follistatin-like 3-Fc-fusion protein and evaluation of its effects on muscle mass in mice (2021)

Follistatin-like 3 (FSTL3) is an endogenous antagonist against transforming growth factor-β family ligands. Monovalent FSTL3-Fc fusion protein (mono-FSTL3-Fc) generated with knobs-into-holes technology overcomes limitations of current anti-myostatin therapies. We have developed a facile protocol for affinity purification of the Fc-fused protein from the supernatant of HEK293T cells stably expressing the protein. This protocol is advantageous by only requiring readily accessible equipment. We further outline the steps for validation of mono-FSTL3-Fc increasing systemic muscle mass in mice after intraperitoneal administration. For complete details on the use and execution of this protocol, please refer to Ozawa et al. (2021).
THE EFFECT OF HYDROGEN PEROXIDE ON YEAST GROWTH AND FERMENTATION (1927)

Summary. Hydrogen peroxide, when added in the form of superoxol to an aerated yeast molasses mash, is very toxic to the yeast, greatly inhibiting or entirely stopping yeast growth, fermentation and inversion. The yeast yield without aeration in a mash of this kind is greatly diminished, while the other changes are only shghtly inhibited, the total loss of solids bekg appreciably greater than with aeration. If the pH change in such an aerated mash is allowed to progress normally, a marked fall from 7.0 to 2.0 is observed, apparently due to the formation of organic acids, and to removal of ammonia from the ammonium salts.
Subject Index Vol. 12, 1999 (1999)

199 Brachyonychia 144 Calcium 132, 257 Capacitance 193 Capsaicin 289 Cell cycle kinetics 174 Chemical enhancer 326 -UV filter 34 Chromametry 85 Cis urocanic acid 18 Coated titanium dioxide 34 Collagen lattice 227 Contact dermatitis 18 Cornified envelope(s) 317 Corticosteroid 199 -concentration 85 Cosmetic ingredients 125 Cosmetics 125 -, bioactive 125 Croton oil 79 Current density 221 Cystic acne
Fast synthesis of exact minimal reversible circuits using group theory (2005)

We present fast algorithms to synthesize exact minimal reversible circuits for various types of gates and costs. By reducing reversible logic synthesis problems to group theory problems, we use the powerful algebraic software GAP to solve such problems. Our algorithms are not only able to minimize for arbitrary cost functions of gates, but also orders of magnitude faster than the existing approaches to reversible logic synthesis. In addition, we show that the Peres gate is a better choice than the standard Toffoli gate in libraries of universal reversible gates.
Synthesis of Chiral Pyridine Bis(Oxazoline) Ligands for Nickel‐Catalyzed Asymmetric Negishi Cross‐Couplings of Secondary Allylic Chlorides with Alkylzincs: 2,6‐Bis[(4 R )‐4,5‐Dihydro‐4‐ (2‐Phenylethyl)‐2‐Oxazolyl]‐ Pyridine (2010)

[Pyridine, 2,6-bis[(4R)-4,5-dihydro-4-(2-phenylethyl)-2-oxazolyl]-].
Neural Networks Based Fractional Pixel Motion Estimation for HEVC (2018)

High Efficiency Video Coding (HEVC) provides more compression than its predecessors. One of the modules that contributes to higher compression rates is the Motion Estimation module, which consists of Integer and Fractional pixel motion estimation. The Fractional Motion Estimation (FME) process performs interpolations to find sample values at fractional-pixel locations, which can be computationally demanding. In this paper, we propose an interpolation-free method for FME based on Artificial Neural Networks (ANNs). Our proposed method is implemented in HEVC reference software (HM-16.9). According to our results, ANNs can accomplish FME task with an average increase of 2.6% in BDRate and an average reduction of 0.09 dB in BD-PSNR.
Incorporating a Psychoacoustical Model in Frequency Domain Speech Enhancement (2004)

A frequency domain optimal linear estimator is proposed which incorporates the masking properties of the human auditory system to make the residual noise distortion inaudible. The use of wavelet-thresholded multitaper spectra is also proposed for frequency-domain speech enhancement methods as an alternative to the traditional fast Fourier transform (FFT)-based magnitude spectra. Experiments with multitalker babble noise indicated that the proposed estimator outperformed the minimum mean-square error log-spectral amplitude estimator (MMSE-LSA), particularly when wavelet-thresholded multitaper spectra were used in place of the FFT spectra.
Deficiencies of Glucosamine-6-Sulfate or Galactosamine-6-Sulfate Sulfatases Are Responsible for Different Mucopolysaccharidoses (1978)

[1-3H]Galactitol-6-sulfate, N- [1-3H]acetylgalactosaminitol-6-sulfate, N-[1-3H]acetylglucosaminitol-6-sulfate, N-acetylglucosamine-6-sulfate, and 6-sulfated tetrasaccharides from chondroitin-6-sulfate have been used for the measurement of 6-sulfatase activity of extracts of normal skin fibroblasts and of fibroblasts cultured from patients with genetic mucopolysaccharidoses. With these substrates, extracts of fibroblasts derived from Morquio patients lack or have greatly reduced activities for galactitol-6-sulfate, N-acetylgalactosaminitol-6-sulfate, and 6-sulfated tetrasaccharides but have normal activity for N-acetylglucosamine-6-sulfate and its alditol; those derived from a patient with a newly discovered mucopolysaccharidosis have greatly reduced activity for N-acetylglucosamine-6-sulfate and its alditol but normal activity for galactitol-6-sulfate, N-acetylgalactosaminitol-6-sulfate, and the 6-sulfated tetrasaccharides. These findings demonstrate the existence of two different hexosamine-6-sulfate sulfatases, specific for the glucose or galactose configuration of their substrates. Their respective deficiencies, causing inability to degrade keratan sulfate and heparan sulfate in one case and keratan sulfate and chondroitin-6-sulfate in the other, are responsible for different clinical phenotypes.
[Effect of GB-115 dipeptide on anxiety in rats with model benzodiazepine withdrawal syndrome]. (2011)

The effects of GB-115 dipeptide, a retroanalog of endogenous CCK-4, on the behavioral indices in "elevated plus maze" (EPM) test and on the content of biogenic amines in the brain structures after discontinuation of a chronic administration of benzodiazepine (BZ) derivatives phenazepam (2.0 mg/kg, i.p.) and diazepam (4.0 mg/kg, i.p.) have been studied in outbred and inbred MR/MNRA rats. It is established that, in 24-48 h following BZ withdrawal, GB-115 dipeptide administered in doses of 0.1 and 0.5 mg/kg, i.p., produced an anxiolytic effect in all animals, which was manifested by increasing the stay time and number of entries in EPM. In the striatum of outbred rats, GB-115 increased DOPAC (+25%) and DA (+31.6%) levels that were decreased during diazepam withdrawal syndrome. The obtained results showed the GB-115 efficiency in attenuating the anxiety caused by BZ withdrawal.
[RuCl3(H2O)n]-Catalyzed Direct Arylations with Bromides as Electrophiles (2007)

Catalytic amounts of [RuCl3(H2O)n] allow for direct ­arylations via C-H bond functionalization with aryl bromides, ­bearing a variety of important functional groups.
ChemInform Abstract: CYCLIC ANALOGS OF DIVINYLSTANNANES, DIVINYLBORANES, AND PENTAHAPTO‐DIVINYLBORANE COMPLEXES (1977)

Abstract Die Dialkine (I) liefern bei der Hydrostannierung die Stannacyclodiene (II).
Y-Branched Multimode/Single-Mode Polymer Optical Waveguides for Low-Loss WDM MUX Device: Fabrication and Characterization (2019)

In this paper, we represent low-loss Y-branched multimode polymer optical waveguides with graded-index (GI) cores applicable to an optical coupler as a MUX device in coarse wavelength division multiplexing links. We apply the Mosquito method as well as the imprint method to form the Y-branched structures in polymers. We experimentally confirm that the insertion loss (including the coupling, propagation, bending, and multiplexing losses) in the multimode GI-core Y-branched waveguide is approximately 1-dB lower than the same Y-branched waveguide with step-index (SI) core. In addition, we also succeeded in fabricating Y-branched single-mode polymer waveguides applying the Mosquito method. In order to satisfy the single-mode condition, the core diameter needs to decrease to 10 mm and less.
Protective Effect of Quinaprilat, an Active Metabolite of Quinapril, on Ca2+-Overload Induced by Lysophosphatidylcholine in Isolated Rat Cardiomyocytes (1999)

We examined the effects of quinaprilat, an active metabolite of quinapril (an angiotensin converting enzyme (ACE) inhibitor) on the increase in intracellular concentration of Ca2+ ([Ca2+]i) (Ca2+-overload) induced by lysophosphatidylcholine (LPC) in isolated rat cardiomyocytes. LPC (15 microM) produced Ca2+-overload with a change in cell-shape from rod to round. Quinaprilat but not quinapril at 20 or 50 microM attenuated the LPC-induced increase in [Ca2+]i and the change in cell-shape in a concentration-dependent manner. Since quinaprilat has an inhibitory action on ACE and quinapril has practically no inhibitory action on ACE, it is likely that the inhibitory action of quinaprilat on ACE is necessary for the protective effect of the drug against LPC-induced changes. We therefore examined the effects of enalapril (another ACE inhibitor with the weak inhibitory action on ACE) and enalaprilat (an active metabolite of enalapril with an inhibitory action on ACE) on the LPC-induced changes. Both enalapril and enalaprilat attenuated the LPC-induced Ca2+-overload, suggesting that the inhibitory action on ACE may not mainly contribute to the protective effect of ACE inhibitors against LPC-induced Ca2+-overload. This suggestion was supported by the fact that neither ACE (0.2 U/ml) nor angiotensin II (0.1-100 microM) increased [Ca2+]i in isolated cardiomyocytes. Furthermore, application of bradykinin (0.01-10 microM) did not enhance the protective effect of quinaprilat against LPC-induced changes. LPC also increased release of creatine kinase (CK) from the myocyte markedly, and quinaprilat but not quinapril attenuated the LPC-induced CK release. Unexpectedly, both enalapril and enalaprilat did not attenuate the LPC-induced CK release. Neither quinapril nor quinaprilat changed the critical micelle concentration of LPC, suggesting that these drugs do not directly bind to LPC. We conclude that quinaprilat attenuates the LPC-induced increase in [Ca2+]i, and that the protective effect of quinaprilat on the LPC-induced change may not be related to a decrease in angiotensin II production or an increase in bradykinin production.
Skin Blood Flow after Intradermal Injection of Ropivacaine in Various Concentrations with and without Epinephrine Evaluated by Laser Doppler Flowmetry (1992)

The combination of ropivacaine and adrenaline did not accentuate but instead diminished the vasoconstrictive effect of epinephrine.
Galanin and Vasopressin Coexist in the Rat Hypothalamo-Neurohypophyseal System (1989)

Using indirect immunofluorescence methods and antisera raised against galanin (GAL) and vasopressin (VP), we have demonstrated both peptides coexisting in the very same cell bodies in the supraoptic and magnocellular paraventricular nuclei and the magnocellular accessory cells of the lateral hypothalamic area. Furthermore, dehydration and salt loading, which is known to cause release and depletion of VP and oxytocin from the neurohypophysis, also caused a marked reduction of GAL-like immunoreactivity in the posterior lobe of the pituitary but had no effect on hypothalamic GAL immunoreactivity. Systemically administered GAL caused a brief small increase in blood pressure with no effect on heart rate. A thousandfold molar concentration of GAL, compared of VP, was required to induce comparable effects on blood pressure. GAL itself had no modulatory effect on VP-induced pressor response. Systemically administered GAL resulted in mild diuresis whereas VP caused complete and sustained inhibition of diuresis. GAL had no effect on VP-induced anti-diuresis effects. The significance of the coexistence and corelease of GAL and VP remains to be elucidated.
Family history of prostate and breast cancer and the risk of prostate cancer in the PSA era (2008)

A family history of PCa or BCa significantly increases PCa risk. These associations are evident in a population with widespread PSA screening.
Endovascular treatment in the multimodality management of brain arteriovenous malformations: report of the Society of NeuroInterventional Surgery Standards and Guidelines Committee (2022)

Although the quality of evidence is lower than for more common conditions subjected to multiple randomized controlled trials, endovascular therapy has an important role in the management of brain AVMs. Prospective studies are needed to strengthen the data supporting these recommendations.
Inhibitory Effect of Zeatin, Isolated from Fiatoua villosa, on Acetylcholinesterase Activity from PC12 Cells (2002)

Acetylcholinesterase (AChE) inhibitors, which enhance cholinergic transmission by reducing the enzymatic degradation of acetylcholine, are the only source of the compound that is currently approved for the treatment of Alzheimer's disease (AD). The methanol extract from Fiatoua villosa among 100 traditional edible plants that were tested, showed the most potent inhibitory effect (51%) on acetylcholinesterase in vitro. After the sequential solvent fractionation of the methanol extract of Fiatoua villosa, the active fraction was repeatedly subjected to open-column chromatography on silica gel. From the highest inhibitory fraction, the chloroform fraction (75%) on AChE, the single compound, was obtained by the Sep-Pak Cartridge (C18: reverse phase column). This compound was finally purified by HPLC (micro-bondapack C18 reverse phase column: 19 x 300 mm). According to the electron impact mass spectrometry (EI-MS), we confirmed that the molecular mass was 219 m/z. The structure of this compound was identified as zeatin [2-methyl-4-(1H-purine-6-ylamino)-2-buten-1-ol], one of the derivatives of purine adenine. The concentration that was required for 50% enzyme inhibition (IC50 value) was 1.09 x 10(-4) M. This study demonstrated that the zeatin from Fiatoua villosa appeared to be the most potent AChE inhibitor in AD.
Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer (2017)

Single cell RNA sequencing has emerged as a powerful tool for resolving transcriptional diversity in tumors, but is limited by throughput, cost and the ability to process archival frozen tissue samples. Here we develop a high-throughput 3' single-nucleus RNA sequencing approach that combines nanogrid technology, automated imaging, and cell selection to sequence up to ~1800 single nuclei in parallel. We compare the transcriptomes of 485 single nuclei to 424 single cells in a breast cancer cell line, which shows a high concordance (93.34%) in gene levels and abundance. We also analyze 416 nuclei from a frozen breast tumor sample and 380 nuclei from normal breast tissue. These data reveal heterogeneity in cancer cell phenotypes, including angiogenesis, proliferation, and stemness, and a minor subpopulation (19%) with many overexpressed cancer genes. Our studies demonstrate the utility of nanogrid single-nucleus RNA sequencing for studying the transcriptional programs of tumor nuclei in frozen archival tissue samples.Single cell RNA sequencing is a powerful tool for understanding cellular diversity but is limited by cost, throughput and sample preparation. Here the authors use nanogrid technology with integrated imaging to sequence thousands of cancer nuclei in parallel from fresh or frozen tissue.
Electrochemical CO2 reduction - The macroscopic world of electrode design, reactor concepts & economic aspects (2022)

For the efficient electrochemical conversion of CO<sub>2</sub> into valuable chemical feedstocks, a well-coordinated interaction of all electrolyzer compartments is required. In addition to the catalyst, whose role is described in detail in the part "Electrochemical CO<sub>2</sub> Reduction toward Multicarbon Alcohols - The Microscopic World of Catalysts & Process Conditions" of this divided review, the general cell setups, design and manufacture of the electrodes, membranes used, and process parameters must be optimally matched. The authors' goal is to provide a comprehensive review of the current literature on how these aspects affect the overall performance of CO<sub>2</sub> electrolysis. To be economically competitive as an overall process, the framework conditions, <i>i</i>.<i>e</i>., CO<sub>2</sub> supply and reaction product treatment must also be considered. If the key indicators for current density, selectivity, cell voltage, and lifetime of a CO<sub>2</sub> electrolyzer mentioned in the techno-economic consideration of this review are met, electrochemical CO<sub>2</sub> reduction can make a valuable contribution to the creation of closed carbon cycles and to a sustainable energy economy.
ChemInform Abstract: Preparation of 2‐Styrylthiazoles from 2‐Methylthiazoles: An Improved Procedure and Mechanistic Aspects. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Recognition Molecules and Immunoglobulin Domains in Invertebratesa (1994)

We have used specific antibody probes to conserved antigenic motifs to identify and characterize immunoglobulin-related molecules in tunicates and a C-type lectin found in lamprey that is related to molecules found in tunicates and mammals. The tunicate immunoglobulin cross-reactive molecule (mu CRM) reacts with antibodies raised to shark IgM heavy chains. Intact tunicate mu CRM is a monomer of Ig light-chain-sized subunits and is oligoclonal by IEF. That this molecule is related to Ig is indicated both by immunochemical data and by peptide sequence homologies. The lamprey lectin is a large polymer (> 500,000 kDa) of 35-kDa and 60-kDa subunits. It appears to be related to C-type lectins as shown by peptide sequence homology and the requirement of Ca2+ for activity. Related molecules appear to be present in tunicates and mammals as shown by cross-reactivity of antibodies in Western blots with single bands from hemolymph and T-cell extracts.
Authalic Parameterization of General Surfaces Using Lie Advection (2011)

Parameterization of complex surfaces constitutes a major means of visualizing highly convoluted geometric structures as well as other properties associated with the surface. It also enables users with the ability to navigate, orient, and focus on regions of interest within a global view and overcome the occlusions to inner concavities. In this paper, we propose a novel area-preserving surface parameterization method which is rigorous in theory, moderate in computation, yet easily extendable to surfaces of non-disc and closed-boundary topologies. Starting from the distortion induced by an initial parameterization, an area restoring diffeomorphic flow is constructed as a Lie advection of differential 2-forms along the manifold, which yields equality of the area elements between the domain and the original surface at its final state. Existence and uniqueness of result are assured through an analytical derivation. Based upon a triangulated surface representation, we also present an efficient algorithm in line with discrete differential modeling. As an exemplar application, the utilization of this method for the effective visualization of brain cortical imaging modalities is presented. Compared with conformal methods, our method can reveal more subtle surface patterns in a quantitative manner. It, therefore, provides a competitive alternative to the existing parameterization techniques for better surface-based analysis in various scenarios.
Unsaturated organometallic compounds of the main group elements. Isolation and structural properties of bis[(tetramethylethylenediamine)lithium(I)] anthracenide (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnsaturated organometallic compounds of the main group elements. Isolation and structural properties of bis[(tetramethylethylenediamine)lithium(I)] anthracenideWendell E. Rhine, J. Davis, and Galen StuckyCite this: J. Am. Chem. Soc. 1975, 97, 8, 2079–2085Publication Date (Print):April 1, 1975Publication History Published online1 May 2002Published inissue 1 April 1975https://doi.org/10.1021/ja00841a014Request reuse permissionsArticle Views171Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (727 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Diagnostic immunocytologique et moléculaire des hémoglobinuries paroxystiques nocturnes (1995)

L 'hemoglobinurie paroxystique nocturne (HPN) est « la seule anemie hemolytique acquise d'origine corpusculaire », apprend-on aux etudiants. Des decouvertes scientifiques recentes ont suscite un nombre croissant de publications sur cette affection hematologique rare. En pratique courante, l'HPN est diagnostiquee dans deux circonstances : la maladie hemolytique [...]
Distinct sets of αβ TCRs confer similar recognition of tumor antigen NY-ESO-1157–165by interacting with its central Met/Trp residues (2008)

Naturally acquired immune responses against human cancers often include CD8(+) T cells specific for the cancer testis antigen NY-ESO-1. Here, we studied T cell receptor (TCR) primary structure and function of 605 HLA-A*0201/NY-ESO-1(157-165)-specific CD8 T cell clones derived from five melanoma patients. We show that an important proportion of tumor-reactive T cells preferentially use TCR AV3S1/BV8S2 chains, with remarkably conserved CDR3 amino acid motifs and lengths in both chains. All remaining T cell clones belong to two additional sets expressing BV1 or BV13 TCRs, associated with alpha-chains with highly diverse VJ usage, CDR3 amino acid sequence, and length. Yet, all T cell clonotypes recognize tumor antigen with similar functional avidity. Two residues, Met-160 and Trp-161, located in the middle region of the NY-ESO-1(157-165) peptide, are critical for recognition by most of the T cell clonotypes. Collectively, our data show that a large number of alphabeta TCRs, belonging to three distinct sets (AVx/BV1, AV3/BV8, AVx/BV13) bind pMHC with equal antigen sensitivity and recognize the same peptide motif. Finally, this in-depth study of recognition of a self-antigen suggests that in part similar biophysical mechanisms shape TCR repertoires toward foreign and self-antigens.