gebhart/patrepeval huggingface dataset:
Hydrogen gas and its role in cell signalling. (2017)

Abstract Hydrogen gas (H 2 ) was once thought to be inert in biological systems but it has now become apparent that exposure of a wide range of organisms, including animals and plants, to H 2 or hydrogen-rich water has beneficial effects. It is involved in plant development, and alleviation of stress and illness, such as reperfusion injury. Here, an overview of how H 2 interacts with organisms is given.
High biological activity of a recombinant protein immobilized onto polystyrene (2009)

The adsorption characteristics of glutathione S-transferases (GST) genetically fused with polystyrene (PS)-binding peptides (PS-tags) on PS plates with increase in hydrophilicity were studied to clarify the mechanisms of the specific interaction between the PS-tag-fused protein and PS plates. GST fused with the PS-tag PS19 (RAFIASRRIKRP) preferentially interacted with hydrophilic PS plates, even in the presence of high concentrations of competitors such as Tween 20 and BSA. Both basic and aliphatic amino acids in the PS-tags were involved in the specific interaction of PS-tags with the surface of the hydrophilic PS plate. Genetic fusion of the PS19 variants, PS19-4 (RAIARRIRR) and PS19-6 (RIIIRRIRR), further improved the immobilization yield of GST in the presence of a high concentration of the competitor BSA (50 mg/mL). The PS19-6 peptide specifically interacted with the surfaces of various hydrophilic PS plates, especially in the presence of Tween 20. Higher remaining activity was detected on all of the hydrophilic PS plates immobilized with GST-PS19-6 in comparison with those with wild-type GST and GST-PS19, and the remaining activity was further increased by the addition of Tween 20 in the adsorption state. The PS19-6 peptide developed in this study is therefore very useful as an affinity tag that can immobilize a target protein directly onto various hydrophilic PS supports with high remaining activity.
ComputationalTechniquesforReachabilityAnalysis ofMax-Plus-LinearSystems ⋆ (2014)

This work discusses a computational approach to reachability analysis of Max-Plus-Linear (MPL) systems, a class of discreteevent systems widely used in synchronization and scheduling applications. Given a set of initial states, we characterize and compute its “reach tube,” namely the collection of set of reachable states (regarded step-wise as “reach sets”). By an alternative characterization of the MPL dynamics, we show that the exact computation of the reach sets can be performed quickly and compactly by manipulations of difference-bound matrices, and further derive worst-case bounds on the complexity of these operations. The approach is also extended to backward reachability analysis. The concepts and results are elucidated by a running example, and we further illustrate the performance of the approach by a numerical benchmark: the technique comfortably handles twenty-dimensional MPL systems (i.e., with twenty continuous state variables), and as such it outperforms the state-of-the-art alternative approaches in the literature.
Adaptation to serum-free culture of HEK 293T and Huh7.0 cells (2014)

Background The fetal bovine serum (FBS) is a component of higher value added to the medium, which may cause difficulty in the process of recovery and purification of bioproduct. Considering all these reasons, it is essential to develop a simple cell adaptation process for the culture of cells in serum-free, animal component-free, or protein-free medium conditions [1,2]. The adaptation of cellular lineages to culture FBS or animal protein free medium can allow optimization of cell growth and expression of heterologous genes, facilitating recovery of recombinant proteins expressed in these cells. Mammalian cell growth can be adapted to serum-free media through progressive reductions of serum concentrations [3]. This gradual reduction over time increases the probability of successful adaptation to low-serum or serum-free mediaby allowing the self-adjustment of the cells to the environment, with the time required depending on the cell line and the composition of the media [4]. Our aim was to establish and to study mammalian cell lines adapted to serum free medium for the expression of recombinant proteins. Thus, we assessed cell growth, nutrient consumption and metabolite production kinetics in the cell culture media tested.
P‐131: Novel Deep Blue Dopants for Organic Light Emitting Devices (2005)

Abstract New deep blue dopants based on a “linked” stilbene moiety have been designed and synthesized. When doped in the stable blue host material, 2‐methyl‐9,10‐di(2‐naphthyl)anthracene (MADN) with a hole blocking layer, the SK‐1 doped blue device achieved a luminance efficiency of 4.7 cd/A with corresponding CIE(inx, y) of (0.145, 0.134) at 20 mA/cm(su2) and an external quantum efficiency of 4.1%.
Novel Hg-free, flat and large Excimer lamps operated by an adaptive pulse gear (2004)

INTRODUCTION Up to now, the lighting industry is using shaped high voltage low pressure mercury discharge tubes behind a coloured plastic plate for advertising. However, plastic plates reduce the system efficiency due to light absorption and scattering losses. Illuminated signage needs flat, large and freely shaped illuminated surfaces showing low degradation. With a fluorescent dielectric barrier discharge (DBD), it is possible to fulfil all these requirements without the use of Hg. In addition, the absence of mercury leads to nearly temperature independent operation of the lamp. In our experiment, we use a highly efficient pulsed Xe excimer discharge to excite an especially designed phosphor inside the DBD-lamp. With a DBD, it is possible to get large areas of high luminance, uniformity and stability [1]. Due to the absence of metal electrodes in the discharge, the lifetime of the lamp is significantly increased and under certain conditions it is mainly limited by the degeneration of the phosphor. The most important demand on illuminated signage is the flexibility of lamp shape. Our lamp design can easily be adapted to illuminate every desired shape. The novel lamp is made of two shaped plates of floated borosilicate glass which are connected using a novel solder technique. In terms of electrical circuit, a DBD represents a capacity which depends on the area of the lamp. To guarantee a stable discharge, independent of the area of the lamp, and to reduce power losses the electronic gear has to be adapted to the capacity of the lamp. Our novel pulse gear is self-adapting to the lamp by adjusting the voltage rise time. This has been realised only by means of switching pattern variation of the full bridge converter.
Evaluation of α‐synuclein as a novel cerebrospinal fluid biomarker in different forms of prion diseases (2016)

Detection of α-synuclein in the CSF of patients with suspected CJD is a valuable diagnostic test reaching almost full discrimination from non-prion disease cases. These data highlight the utility of CSF α-synuclein quantification in front of classical CSF biomarkers in clinical routine.
Optically Active Elastomers from Liquid Crystalline Comb Copolymers with Dual Physical and Chemical Cross-Links (2017)

We report on the synthesis and properties of cholesteric liquid crystalline random terpolymers with comblike architecture as a modular platform for preparation of stimuli-responsive photonic elastomers. Ring-opening metathesis of norbornene monomers bearing n-alkyloxy cholesteryl (Ch9), n-alkoxy cyanobiphenyl (CB6 or CB12), and poly(ethylene glycol) (PEG) side chains is efficient and quantitatively yields low polydispersity random terpolymers. This terpolymer scaffold self-assembles to form cholesteric mesophases (N*) in which microphase-segregated domains of PEG side chains are randomly embedded. The cholesteric mesophase provides a 1D photonic band gap structure at optical wavelengths, which is maintained during chemical cross-linking of the norbornene backbone to form elastomers. The presence of cyanobiphenyl mesogens leads to an increase in the helical pitch of the cholesteric mesophase, resulting in a red-shift of the reflectivity relative to the pure cholesteric mesophase. By contrast, the presence of PEG blue-shifts the reflectivity, such that the overall optical properties can be readily tailored by the composition of the terpolymer. Furthermore, the mechanical properties of the materials are enhanced by the presence of the microphase-separated PEG domains which act as physical cross-links and also provide plasticization of the system. The terpolymers described here provide a modular and versatile platform for the realization of photopatternable materials that exhibit shape memory and thermochromic properties.
Continuous Vital Sign Analysis to Predict Secondary Neurological Decline After Traumatic Brain Injury (2018)

<b>Background:</b> In the acute resuscitation period after traumatic brain injury (TBI), one of the goals is to identify those at risk for secondary neurological decline (ND), represented by a constellation of clinical signs that can be identified as objective events related to secondary brain injury and independently impact outcome. We investigated whether continuous vital sign variability and waveform analysis of the electrocardiogram (ECG) or photoplethysmogram (PPG) within the first hour of resuscitation may enhance the ability to predict ND in the initial 48 hours after traumatic brain injury (TBI). <b>Methods:</b> Retrospective analysis of ND in TBI patients enrolled in the prospective Oximetry and Noninvasive Predictors Of Intervention Need after Trauma (ONPOINT) study. ND was defined as any of the following occurring in the first 48 h: new asymmetric pupillary dilatation (>2 mm), 2 point GCS decline, interval worsening of CT scan as assessed by the Marshall score, or intervention for cerebral edema. Beat-to-beat variation of ECG or PPG, as well as waveform features during the first 15 and 60 min after arrival in the TRU were analyzed to determine physiologic parameters associated with future ND. Physiologic and admission clinical variables were combined in multivariable logistic regression models predicting ND and inpatient mortality. <b>Results:</b> There were 33 (17%) patients with ND among 191 patients (mean age 43 years old, GCS 13, ISS 12, 69% men) who met study criteria. ND was associated with ICU admission (<i>P</i> < 0.001) and inpatient mortality (<i>P</i> < 0.001). Both ECG (AUROC: 0.84, 95% CI: 0.76,0.93) and PPG (AUROC: 0.87, 95% CI: 0.80, 0.93) analyses during the first 15 min of resuscitation demonstrated a greater ability to predict ND then clinical characteristics alone (AUROC: 0.69, 95% CI: 0.59, 0.8). Age (<i>P</i> = 0.02), Marshall score (<i>P</i> = 0.001), penetrating injury (<i>P</i> = 0.02), and predictive probability for ND by PPG analysis at 15 min (<i>P</i> = 0.03) were independently associated with inpatient mortality. <b>Conclusions:</b> Analysis of variability and ECG or PPG waveform in the first minutes of resuscitation may represent a non-invasive early marker of future ND.
On-chip learning of FPGA-inspired neural nets (2002)

Neural networks are usually considered as naturally parallel computing models. But the number of operators and the complex connection graphs of standard neural models can not be handled by digital hardware devices. A new theoretical and practical framework allows to reconcile simple hardware topologies with complex neural architectures: field programmable neural arrays (FPNA) lead to powerful neural architectures that are easy to map onto digital hardware, thanks to a simplified topology and an original data exchange scheme. The paper focuses on a class of synchronous FPNAs, for which an efficient implementation with on-chip learning is described. Application and implementation results are discussed.
Holoscopy—holographic optical coherence tomography (2011)

Scanning optical coherence tomography (OCT) is limited in sensitivity and resolution by the restricted focal depth of the confocal detection scheme. Holoscopy, a combination of holography and Fourier-domain full-field OCT, is proposed as a way to detect photons from all depths of a sample volume simultaneously with uniform sensitivity and lateral resolution, even at high NAs. By using the scalar diffraction theory, as frequently applied in digital holographic imaging, we fully reconstruct the object field with depth-invariant imaging quality. In vivo imaging of human skin is demonstrated with an image quality comparable to conventionally scanned OCT.
Abstract 3499: Novel Nitric Oxide-Containing Echogenic Liposome for the Treatment of Vasospasm Following Subarachnoid Hemorrhage (2012)

Background: Cerebral vasospasm following aneurysmal subarachnoid hemorrhage (SAH) is a major cause of high morbidity and mortality. The reduced availability of nitric oxide (NO) in blood and cerebrospinal fluid (CSF) is suggested an important mechanism underlying cerebral vasospasm, a complication of SAH. NO or NO donors are known to have potent effects on cerebral vascular relaxation. We have developed NO-containing echogenic liposomes (NO-ELIP) for NO delivery to assess the role of NO in SAH-induced vasospasm. We hypothesized that NO-ELIP can produce vasodilation and provide an effective therapeutic approach to treat vasospasm following SAH. Methods: NO-ELIP was created by a freeze-under-pressure method. Male Sprague- Dawley rats (n=9) underwent SAH by puncturing the right middle cerebral artery with a sharp tip nylon monofilament. In the treatment groups, NO-ELIP were administered through the right femoral vein at 24 hours after SAH onset. The brain tissue was obtained for H&amp;E staining at 30 min after NO-ELIP administration. Measurement of the posterior communicating artery was performed to evaluate the effects of NO-ELIP treatment on vasospasm following SAH. Results: After SAH, the normalized lumenal and wall thickness areas decreased from 1.02 ± 0.08 to 0.40 ± 0.15 (p&lt;0.001). NO-ELIP inhibited SAH-induced vasospasm by 67% (normalized area decreased to 0.82 ± 0.10 (p=0.004). Conclusion: This study demonstrates that NO-ELIP have the potential to deliver NO for therapeutic treatment of vasospasm. Enhancing the release of NO from circulating NO-ELIP by combining ultrasound exposure over the carotid artery may further provide an improved treatment strategy. This suggests that targeted therapeutic gas can be delivered to the cerebral circulation for the treatment of vasospasm following SAH
ChemInform Abstract: CONVERSION OF ALLYLPHOSPHINE TO NOVEL DISPHOSPHINES: SYNTHESIS AND CHARACTERIZATION OF CH2:CHCH2P(H)(CH2)3PH2 AND P((CH2)3)3P (1982)

Abstract Die Photoreaktion von Allylphosphin (I) in der Gasphase liefert das P‐Allyl‐propylendiphosphin (II), die Radikalreaktion von (I) mit Azoisobuttersäuredinitril (III) neben PH 3 das 1,5‐Diphosphabicyclo[3.3.3]undecan (IV).
Suitability of RAPD analysis for the detection of somaclonal variants in oil palm (Elaeis guineensis Jacq) (1998)

Abstract Random amplified polymorphic DNA (RAPD) analysis using arbitrary 10‐mer oligonucleotide primers was employed in order to investigate the genetic fidelity of somatic embryogenesis‐derived regenerants of oil palm ( Elaeis guineensis Jacq). Clonal palms bearing the ‘mantled’ phenotype were identified in the field and the ability of RAPD markers to distinguish these variants from palms of the normal type was assessed. Of the 387 arbitrary primers used, 259 (67%) were successfully used to amplify oil palm DNA genomic fragments with consistently reproducible banding. Of these 387 primers, 73 (19%) primers enabled the identification of polymorphism between clones. No intraclonal variability and no differences between mother palms and regenerants could be identified using the total number of markers scored (8900). Twenty‐four of these 73 primers were chosen for use in a larger experiment aimed at comparing, first, the mother palm genome with that of its clonal offspring and, second, true‐to‐type and variant regenerants. Thus, the regeneration protocol based on somatic embryogenesis set up for oil palm clonal propagation does not induce any gross genetic changes. The results obtained revealed however, that the RAPD approach is not suitable for the detection of the ‘mantled’ variant phenotype. The use of RAPD markers for the detection of somaclonal variation in oil palm is discussed.
Drug Delivery to Anterior Segment of the Eye (2013)

Transport of therapeutic agents into the anterior segment of the eye is highly restricted by various anatomical and physiological barriers. Topical administration is the most convenient method of drug delivery for the treatment of anterior segment eye diseases. The global market for eye care products is approximately $12.5 billion and is growing at a rate of 9% every year. Eye drops account for 90% of all conventional ophthalmic formulations. However, it suffers from several disadvantages such as nasolacrimal drainage, loss in conjunctival blood circulation, tear dilution, normal tear drainage and reflux blinking. There is a need for alternate drug delivery systems which can address poor ocular absorption associated with conventional drug delivery systems. In this chapter, we have made an attempt to briefly describe various drug delivery systems employed in the treatment of anterior segment eye diseases such as nanoemulsions, collagen corneal shields, hydrogels, vesicular systems (liposomes and niosomes), iontophoresis, phonophoresis, punctal plugs and contact lenses.
Turbo Decoding with Run Length Limited Code for Optical Storage (2002)

A new turbo decoding method with a run length limited (RLL) code for optical storage is proposed. The system entails a trellis code that joins the RLL code, the non-return-to-zero inverse (NRZI) conversion, and the partial response (PR) channel as the inner code. The inner a posteriori probability (APP) decoder is able to utilize the extrinsic information fed back from the output of the outer decoder. A turbo-coded (1, 7)RLL-constrained PR2 channel was simulated. The full, partial, and serial turbo decoding systems with the inner APP decoder achieved coding gains of 4.7 dB, 4.3 dB, and 3.3 dB, respectively, over the uncoded (1, 7)RLL-constrained PR2 channel at a bit error rate (BER) of 10-5. The full and partial turbos were also improved by 1.2 dB and 0.8 dB, respectively, over the partial turbo with the RLL soft-input soft-output (SISO) decoder at a BER of 10-5.
Wrist movements induce torque and lever force in the scaphoid: an ex vivo study (2020)

In unstable scaphoid fractures, we suggest securing rotational stability with selected implants for functional postoperative care. Wrist ROM within 20% extension and radial abduction to 50% flexion limit torque and lever force exacerbation between scaphoid fragments.
Lunar Rover Localization Using Craters as Landmarks (2022)

Onboard localization capabilities for planetary rovers to date have used relative navigation, by integrating combinations of wheel odometry, visual odometry, and inertial measurements during each drive to track position relative to the start of each drive. At the end of each drive, a &#x201C;ground-in-the-loop&#x201D; (GITL) interaction is used to get a position update from human operators in a more global reference frame, by matching images or local maps from onboard the rover to orbital reconnaissance images or maps of a large region around the rover&#x0027;s current position. Autonomous rover drives are limited in distance so that accumulated relative navigation error does not risk the possibility of the rover driving into hazards known from orbital images. In practice, this limits drives to a few hundred meters between GITL cycles. Several rover mission concepts have recently been studied that require much longer drives between GITL cycles, particularly for the Moon. This includes lunar rover mission concepts that involve (1) driving mostly in sunlight at low latitudes, (2) driving in permanently shadowed regions near the south pole, and (3) a mixture of day and night driving in mid-latitudes. These concepts include total traverse distance requirements of up to 1,800 km in 4 Earth years, with individual drives of several kilometers between stops for downlink. These concepts require greater autonomy to minimize GITL cycles to enable such large range; onboard global localization is a key element of such autonomy. Multiple techniques have been studied in the past for onboard rover global localization, but a satisfactory solution has not yet emerged. For the Moon, the ubiquitous craters offer a new possibility, which involves mapping craters from orbit, then recognizing crater landmarks with cameras and/or a lidar onboard the rover. This approach is applicable everywhere on the Moon, does not require high resolution stereo imaging from orbit as some other approaches do, and has potential to enable position knowledge with order of 5 to 10 m accuracy at all times. This paper describes our technical approach to crater-based lunar rover localization and presents initial results on crater detection using 3-D point cloud data from onboard lidar or stereo cameras, as well as using shading cues in monocular onboard imagery.
When Satellites Attack: Satellite-to-Satellite Cyber Attack, Defense and Resilience (2020)

The United States is increasingly reliant on space systems for civil and military operations. Therefore, it is no surprise that adversaries are interested in compromising these systems via cyber attack. This paper describes a new class of satellite-to-satellite cyber attacks. While such attacks were previously limited to a select group of nation-states, low-cost cubesats and ground station cloud services make these attacks increasingly feasible and accessible to adversaries. There are no publicly documented instances of satellite-to-satellite cyber attacks occurring at the time of publication, but the technical feasibility is described herein along with proposed defense and resilience techniques. Policy recommendations to help manage the risk of satellite-to-satellite attacks are also discussed.
Semantic Matching, Propagation and Transformation for Composition in Component-Based Systems (2009)

Composition of software applications from component parts in response to high-level goals is a long-standing and highly challenging goal. We target the problem of composition in flow-based information processing systems and demonstrate how application composition and component development can be facilitated by the use of semantically described application metadata. The semantic metadata describe both the data flowing through each application and the processing performed in the associated application code. In this paper, we explore some of the key features of the semantic model, including the matching of outputs to input requirements, and the transformation and the propagation of semantic properties by components