gebhart/patrepeval huggingface dataset:
Solanaceae as a model system for the molecular characterization of hosts factors required for potyvirus infection (2004)

International audience
Targeting hepatitis B vaccine escape using immunogenetics in Bangladeshi infants (2023)

Hepatitis B virus (HBV) vaccine escape mutants (VEM) are increasingly described, threatening progress in control of this virus worldwide. Here we studied the relationship between host genetic variation, vaccine immunogenicity and viral sequences implicating VEM emergence. In a cohort of 1,096 Bangladeshi children, we identified human leukocyte antigen (HLA) variants associated with response vaccine antigens. Using an HLA imputation panel with 9,448 south Asian individuals <i>DPB1*04:01</i> was associated with higher HBV antibody responses (p=4.5×10<sup>-30</sup>). The underlying mechanism is a result of higher affinity binding of HBV surface antigen epitopes to DPB1*04:01 dimers. This is likely a result of evolutionary pressure at the HBV surface antigen 'a-determinant' segment incurring VEM specific to HBV. Prioritizing pre-S isoform HBV vaccines may tackle the rise of HBV vaccine evasion.
Beamformer characterization of the MIR instrument: The microwave interferometric reflectometer (2017)

Global Navigation Satellite Systems - Reflectometry (GNSS-R) has opened promising results for remote sensing applications. The broadcast of new signals with wider bandwidth provides an opportunity to increase the performance of GNSS reflectometry applications. The Microwave Interferometric Reflectometer (MIR) is an instrument being developed to compare different GNSS-R techniques, namely the conventional and interferometric ones, and assess the capabilities of these new signals and bands of the GPS and Galileo systems. This work describes the tests to validate the beamforming system.
Clonal expansion and attenuated apoptosis in Wilms' tumors are associated with p53 gene mutations. (1995)

The p53 gene product is required for activation of an apoptotic pathway triggered by oncogenes and cytotoxic agents. Wilms' tumor, a pediatric renal malignancy, provides a paradigm for evaluating genetic events involved in tumor progression. This malignancy is generally not associated with p53 mutations, and even in advanced disease states is quite responsive to current treatment regimens. The anaplastic histological variant of Wilms' tumor, however, is frequently associated with p53 gene mutations and shows poor prognosis. We analyzed seven Wilms' tumors for which we had paired samples from nonanaplastic and anaplastic regions. p53 mutations were detected in six of these tumors, five of which demonstrated mutations restricted to anaplastic regions. Nonanaplastic cells of the sixth sample were heterozygous for a p53 mutation, whereas the anaplastic area of this tumor showed reduction to homozygosity. These results indicate that progression to anaplasia is associated with clonal expansion of cells which have acquired a p53 mutation. We demonstrated that tumor cells with p53 mutations show attenuated apoptosis, suggesting that such lesions may provide a selective advantage in vivo by decreasing cell death.
Identification of factor VIII gene mutations in 101 patients with haemophilia A: mutation analysis by inversion screening and multiplex PCR and CSGE and molecular modelling of 10 novel missense substitutions (2005)

Haemophilia A (HA) is an X-linked bleeding disorder caused by diverse mutations in the human coagulation factor VIII (FVIII) gene. We have analysed DNA from 109 unrelated Indian patients with HA for their FVIII gene defects. Among these patients 89 (82%) had severe (FVIII:C <1%) HA, 11 (10%) had moderate (FVIII:C 1-5%) HA and nine (8%) had mild (FVIII:C 5-30%) HA. These patients were first screened for the common intron 22 and intron 1 inversions. Inversion negative samples were screened for point mutations by a multiplex PCR and conformation sensitive gel electrophoresis strategy. Mutations were identified in 101 of the 109 patients. These included two (2%) intron 1 and 51 (51%) intron 22 inversions, four (4%) gross deletions and 44 (43%) point mutations. Twenty-nine novel causative mutations, including 11 missense, seven frameshift, five nonsense mutations, three splice site defects and three gross deletions were detected. Ten of the novel missense mutations were studied by molecular modelling. Two different (Thr2253Pro and Pro1392fs) mutations were seen in four unrelated families and FVIII gene haplotyping suggested a common founder effect. Seven of these 109 patients had inhibitors. Among them, four had intron 22 inversions, one had a novel gross deletion (delexon 2-9) and one a nonsense mutation (Trp1535Stop). In one of these patients, no mutation could be identified in the FVIII gene. A Thr2253Pro novel mutation and an intron 22 inversion were identified in two female haemophiliacs. The data from this study suggests that the spectrum of gene defects in Indian patients with HA is as heterogeneous as reported in other populations.
Genomics and Privacy: Implications of the New Reality of Closed Data for the Field (2011)

Open source and open data have been driving forces in bioinformatics in the past. However, privacy concerns may soon change the landscape, limiting future access to important data sets, including personal genomics data. Here we survey this situation in some detail, describing, in particular, how the large scale of the data from personal genomic sequencing makes it especially hard to share data, exacerbating the privacy problem. We also go over various aspects of genomic privacy: first, there is basic identifiability of subjects having their genome sequenced. However, even for individuals who have consented to be identified, there is the prospect of very detailed future characterization of their genotype, which, unanticipated at the time of their consent, may be more personal and invasive than the release of their medical records. We go over various computational strategies for dealing with the issue of genomic privacy. One can "slice" and reformat datasets to allow them to be partially shared while securing the most private variants. This is particularly applicable to functional genomics information, which can be largely processed without variant information. For handling the most private data there are a number of legal and technological approaches-for example, modifying the informed consent procedure to acknowledge that privacy cannot be guaranteed, and/or employing a secure cloud computing environment. Cloud computing in particular may allow access to the data in a more controlled fashion than the current practice of downloading and computing on large datasets. Furthermore, it may be particularly advantageous for small labs, given that the burden of many privacy issues falls disproportionately on them in comparison to large corporations and genome centers. Finally, we discuss how education of future genetics researchers will be important, with curriculums emphasizing privacy and data security. However, teaching personal genomics with identifiable subjects in the university setting will, in turn, create additional privacy issues and social conundrums.
Acoustic micromanipulation using a multi-electrode transducer (2002)

Control of position of particles using acoustic radiation pressure in water was studied to develop noncontact micromanipulation technique. The radiation pressure traps particles suspended in water and forms agglomeration every half wavelength in an ultrasonic standing wave field. This paper describes a method to transport them in the transverse direction to the sound beam axis. The principle is based on the shift of sound field by driving selected elements of a multielectrode transducer. The transducer was a rectangle 20/spl times/40 mm in size with fifteen electrodes of 20/spl times/2 mm. Driving a series of electrodes of the transducer, alumina particles were trapped at the nodes of the standing wave field. When the driving electrodes were switched sequentially, the standing wave field shifted laterally and the trapped particles moved to the corresponding nodal points. The resolution of transportation can be made smaller by reducing the size of electrodes. This suggests a new technique to manipulate micro-objects without contact using an ultrasonic standing wave field generated by a multielectrode transducer.
Mathematische Beschreibungen (2002)

Die Komplexität wird hier einheitlich mit K abgekürzt, wobei die unterschiedlichen Disziplinen bei den untenstehenden Definition normalerweise nicht an Komplexität denken, sondern an Entropie, Information, ...
Venous Thromboembolism Among Elderly Patients Treated With Atypical and Conventional Antipsychotic Agents (2005)

Atypical antipsychotic agents appear to increase the risk of VTE. However, these events are rare, and in clinical practice the absolute risk should be weighed against the effectiveness of these medications in the elderly population.
Carryover of [imazapic + imazapyr] on differents crops systems in lowland areas with ALS tolerant rice (2022)

This study aimed to assess the impact of the herbicide associated to the Clearfield® technology of rice cultivation on ryegrass planted in succession, as well as on both non?Clearfield® rice and soybean planted in rotation. Was installed in the Lowlands Experimental Field, Embrapa Clima Temperado, Capão do Leão, Brazil, from 2016 to 2018. The experiment comprised the application of Kifix® (imazapic + imazapyr) to Clearfield® rice grown at the first experimental year, evaluating its impact on the Clearfield® rice (1st summer), on ryegrass grown on the succeeding winter, as well as on soybean and non?Clearfield® rice grown on the following summer cropping season (2nd summer). The randomized blocks design was used with four replications, with plots measuring 72&nbsp;m2 at the first cropping season and winter cropping, being later split (32&nbsp;m2) for the second summer cropping season, to allow planting both non?Clearfield® rice and soybean. Our experiments reported mild damages from Kifix® residues on ryegrass planted in succession to Clearfield® rice, as well as on non?tolerant rice and soybean in rotation to Clearfield® rice, under lowland areas of Southern Brazil. Furthermore, the possibility of accumulation of Kifix® residues in soil, after repeated applications should be verified in further studies.
Tetraspanin CD82 controls the association of cholesterol-dependent microdomains with the actin cytoskeleton in T lymphocytes: relevance to co-stimulation (2004)

T-cell activation is initiated by the concerted engagement of the T-cell receptor and different co-stimulatory molecules, and requires cytoskeleton-dependent membrane dynamics. Here, we have studied the relationships between tetraspanins, cytoskeleton and raft microdomains, and their relevance in T-cell signaling. Localization studies and density-gradient flotation experiments indicate that part of tetraspanins localizes in raft microdomains linked to the actin cytoskeleton. First, partial coalescence of lipid raft is triggered by tetraspanin cross-linking and results in large caps in which F-actin also concentrates. Second, the amount of tetraspanins, which are recovered in the cholesterol-dependent insoluble fractions of low and intermediate density, and which appears to be membrane vesicles by electron microscopy, is under cytoskeletal influence. Disruption of actin filaments enhances the amount of tetraspanins recovered in typical raft fractions, whereas F-actin-stabilizing agents induce the opposite effect. Our data also reveal that CD82 constitutes a link between raft domains and the actin cytoskeleton, which is functionally relevant. First, tetraspanin signaling induces a selective translocation of CD82 from detergent-resistant membrane fractions to the cytoskeleton-associated pellet. Second, all functional effects linked to CD82 engagement, such as adhesion to culture plates, formation of actin bundles and early events of tyrosine phosphorylation, are abolished, or strongly reduced, by cholesterol depletion. We also show that dynamic relocalization of CD82 and F-actin at the periphery of the immune synapse is induced upon contact of T cells with antigen-presenting cells. This suggests that the tetraspanin web might participate in the membrane dynamics required for proper T-cell signaling. More generally, the interaction of tetraspanins with raft domains and with the actin cytoskeleton might relate with their role in many cellular functions as membrane organizers.
Joint Second Prize Significance of Y and Cr in TiAlN Hard Coatings for Dry High Speed Cutting (2001)

High speed cutting of die steels (A2, HRC58, 15 000 rev min -1 , 385 m min -1 ) demands high wear and oxidation resistant and extremely well adherent coatings. PVD is the preferred deposition technique to tailor a coating for this application. With the development of TiAlN based hard coatings, an important step in the direction of improved cutting performance has been achieved. TiAlCrYN is a state of the art coating, which has proven to be very successful in demanding dry high speed cutting operations. A special feature of this coating is a double layered overcoat consisting of a lower fine multilayered TiAlYN/CrN with a periodicity of 1·6 nm graded into an upper glassy amorphous TiAlY oxynitride. The incorporation of Y leads to an enhanced oxidation resistance, whereas the most important effect of the double layered overcoat is the reduction of the friction coefficient. To confirm the beneficial effects of Y and Cr, sophisticated TEM has been employed with an X-ray resolution of approxi mately 3 nm. Segregation of Y and other elements in this system (tool/coating) were detected along the column boundaries in the TiAlCrYN coating at 900°C. Y is acting like a ‘plug’ against both inward and outward diffusion of foreign elements. The oxidation behaviour has been further quantified using highly sensitive thermogravimetric analysis and glancing angle X-ray diffraction. The successful impact of the incorporation of Y into TiAlCrN and the use of a Cr containing nanostructured oxynitride sputter deposited overcoat is shown by coated two flute ball nosed solid carbide cutters at a cutting speed of 385 m min -1 .
Omissions in Vol. I (2011)

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Antibody-independent B cell effector functions in relapsing remitting Multiple Sclerosis: Clues to increased inflammatory and reduced regulatory B cell capacity (2012)

The pathogenic role for B cells in the context of relapsing remitting multiple sclerosis (MS) is incompletely defined. Although classically considered a T cell-mediated disease, B cell-depleting therapies showed efficacy in treating the clinical symptoms of RRMS without decreasing plasma cells or total immunoglobulin (Ig) levels. Here, we discuss the potential implications of antibody-independent B cell effector functions that could contribute to autoimmunity with particular focus on antigen presentation, cytokine secretion, and stimulation of T cell subsets. We highlight differences between memory and naïve B cells from MS patients such as our recent findings of hyper-proliferation from MS memory B cells in response to CD40 engagement. We discuss the implications of IL6 overproduction in contrast to limited IL10 production by B cells from MS patients and comment on the impact of these functions on yet unexplored aspects of B cells in autoimmune disease. Finally, we contextualize B cell effector functions with respect to current immunomodulatory therapies for MS and show that glatiramer acetate (GA) does not directly modulate B cell proliferation or cytokine secretion.
Photodynamic Therapy for Actinic Keratoses (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Dermatology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Dermatology journal
Effects of synergism and multistep heating in graphene–polypyrrole–polymethyl methacrylate composites on their electrical and mechanical properties in additive manufacturing (2020)

A highly electrically conductive hybrid multicomponent graphene–polymer composite was produced by solvent casting method with subsequent melt blending processes. As per the experimental design, the composite had to undergo multiple heating steps from solvent casting to extrusion to 3D-printing, the effects of processing conditions, mainly temperature, on its mechanical and electrical properties and the mechanisms behind the changes had to be understood. It has been noted that there is a significant increase in mechanical properties from extruded strand (tensile strength [48 MPa] and flexural strength [27 MPa]) to 3D-printed samples (tensile strength [67 MPa] and flexural strength [61 MPa]). An electrical conductivity of 11.4 S cm−1 was measured for the as-synthesised composite before melt processing. However, the conductivity does not change noticeably until 3D-printing. From the thermogravimetric analysis, it is clear that there is no change in the functionalities of extruded strands and 3D-printed sample, but energy dispersive spectroscopy analysis showed that there is a significant reduction in agglomerates that played a critical factor in improving the homogeneity of the dispersion.
Parity-distribution: a shortcut to reliable cluster computing system (2000)

This paper presents a device level approach to provide high availability of storage systems to an existing cluster computing system with minimal cost both in hardware and software. The parity-distribution scheme is used to place parity information across the network. After adopting the method, an existing system can tolerate and recover from disk and cluster node failures by enhanced disk drivers. Compared with previous work, this approach provides an attractive shortcut to realize higher availability, lower cost of design and implementation, better compatibility for applications and higher expandability.
Dual Reflectarray Ka-band Multibeam Antenna (2019)

This paper describes a Ka-band multibeam antenna systems for a generic Continental US (CONUS) coverage. The configuration is implemented with two dual offset reflectarray antennas, one antenna works in transmit and another in receive. Each reflectarray antenna consists on a flat reflectarray as sub-reflector and a flat reflectarray as main-reflector. This antenna topology is a reduced cost alternative to a standard parabolic reflector antenna system implemented with four reflectors, also described in the text.
Evaluation of Voltage Sag Ride-Through of a Doubly Fed Induction Generator Wind Turbine with Series Grid Side Converter (2007)

With steadily increasing wind turbine penetration, regulatory standards for grid interconnection have evolved to require that wind generation systems ride- through disturbances such as faults and support the grid during such events. Conventional ride-through accommodations result in high currents and torque or are unable to ride-through down to zero volts. This paper explores two DFIG configurations in which a series grid side converter (SGSC) is employed exclusively for voltage sag ride-through. A control strategy applicable to both configurations is developed whereby the SGSC engages to stabilize the stator flux at the beginning and resolution of the sag event. Simulation results show excellent zero voltage ride-through response.
Daily patient-reported assessment of pain, anxiety, or distress, problems or concerns, and need to see physician during 100 courses of radiation therapy. (2014)

193 Background: Patients (pts) undergoing radiation therapy (RT) treatment (Rx) may have pain, anxiety or distress (AoD), or other problems at the time of initiation of RT. Pts may also develop or have an exacerbation of one or more problems during RT. While undergoing RT, pts are seen weekly by the treating physician (MD), and seen daily by the treating radiation therapy technologists (RTT). Pts can develop problems between weekly MD visits which may benefit from expedited interventions. A protocol was developed where pts would be asked four questions each treatment day by the RTT: 1) Are you having pain, and if so, how would you rate it on a scale of zero to 10? (PainQ) 2) Are you having anxiety or distress today? (ADQ) 3) Are you having new problems or concerns? (ProConQ) 4) Do you want to see the physician today? (CMDQ) This information was charted, and used in pt management. Methods: 100 consecutive pt charts were reviewed for demographics including gender, age, primary malignancy, region treated, number of Rx and radiation dose. Daily answers from the four question instrument were also reviewed for each pt course of RT. Results: Of the 100 pt, 56 were male. Disease distribution: Breast-22; Prostate-20; Head and Neck-20; Lung-13; Brain-7; Other-18. For the ADQ, 83 did not report anxiety or distress; 17 reported AoD on 1-2 occasions. For the ProConQ, 35 reported none, 34 reported yes on 1-2 occasions, 22 reported yes on 3-5 occasions, and 9 reported yes on &gt;5 occasions. 29 reported yes on 2 days in a row, and 15 reported yes on 3 days in a row. For the PainQ, 31 reported no pain throughout their course of RT, with 22, 23, and 25 reporting pain of any magnitude for 1 to 5, 6 to10, and &gt;10 days, respectively. 22 reported pain level &gt; 6 on at least one occasion. For CMDQ, 26 pts did not request to see the MD greater than the usual weekly visit, 71 and 3 requested to see the MD an additional 1 to 5, and 6 to 10 times. Conclusions: A simple four question daily query of pts undergoing a course of RT is feasible, and may prompt the need for evaluation and interventions between scheduled MD visits. This may facilitate more expeditious interventions for Rx related toxicity and treatment-dependent or independent symptoms or needs.