gebhart/patrepeval huggingface dataset:
Amelioration of intracellular Ca2+ regulation by exon-45 skipping in Duchenne muscular dystrophy-induced pluripotent stem cell-derived cardiomyocytes (2019)

Duchenne muscular dystrophy (DMD) is a devastating muscle disorder caused by frameshift mutations in the DMD gene. DMD involves cardiac muscle, and the presence of ventricular arrhythmias or congestive failure is critical for prognosis. Several novel therapeutic approaches are being evaluated in ongoing clinical trials. Among them, exon-skipping therapy to correct frameshift mutations with antisense oligonucleotides is promising; however, their therapeutic efficacies on cardiac muscle in vivo remain unknown. In this study, we established induced-pluripotent stem cells (iPSCs) from T cells from a DMD patient carrying a DMD-exon 46-55 deletion, differentiated the iPSCs into cardiomyocytes, and treated them with phosphorodiamidate morpholino oligomers. The efficiency of exon-45 skipping increased in a dose-dependent manner and enabled restoration of the DMD gene product, dystrophin. Further, Ca<sup>2+</sup>-imaging analysis showed a decreased number of arrhythmic cells and improved transient Ca<sup>2+</sup> signaling after exon skipping. Thus, exon-45 skipping may be effective for cardiac involvement in DMD patients harboring the DMD-exon 46-55 deletion.
Detection of HIV-1 resistance genotypes in patients from Tygerberg Hospital with treatment failure, by using an In-House- and a commercial resistance genotyping assay (2005)

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Transcriptional profiling of human monocytes reveals complex changes in the expression pattern of inflammation-related genes in response to the annexin A1-derived peptide Ac1-25 (2007)

Annexin A1 is a glucocorticoid-regulated, anti-inflammatory protein, which plays an important role as an endogenous regulator of the inflammatory response. Many of these anti-inflammatory properties are retained in the N-terminal annexin A1 peptide Ac1-25, which is released from the full-length protein by a neutrophil elastase. To elucidate whether the anti-inflammatory activity of the bioactive peptide is solely a result of immediate post-translational effects, which include the shedding of L-selectin or also involve transcriptional changes affecting leukocyte function, we recorded global gene expression changes in human monocytes stimulated with exogenously applied Ac1-25. Applying stringent selection criteria, we show that approximately 100 genes are up-regulated, and approximately 230 are down-regulated by a factor of at least two in the Ac1-25-treated monocytes. It is important that the profiling reveals that Ac1-25 induces an anti-inflammatory phenotype by down-regulating proinflammatory and up-regulating anti-inflammatory mediators. These effects, elicited by exogenously applied Ac1-25, depend, to different extents, on ERK1/2 and p38 signaling pathways. This identifies the annexin A1 N-terminal peptide as a stimulus, eliciting not only short-term, post-translational effects in human monocytes but also transcriptional changes, defining a more anti-inflammatory profile.
ChemInform Abstract: Sodium/V6O13 Polymer Electrolyte Cells. (1989)

Abstract The electrochemical behavior of an all solid state cell utilizing a Na anode, a polyethylene oxide (PEO) based electrolyte (NaCF3SO3/PEO) and a V 6 O 13 insertion cathode is studied.
The efficacy of three metaldehyde pellets marketed in Argentina, on the control of Deroceras reticulatum (Müller) (Pulmonata: Stylommatophora) (2008)

Management of the grey slug, Deroceras reticulatum (Müller) (Pulmonata: Stylommatophora), in Argentina, has been limited to the use of pelleted toxic baits containing metaldehyde. The most important molluscicides in the Argentinean market are Clartex (Rizobacter Argentina S.A.), Acay (Acay Agro S.R.L.) and Babotox (Huagro S.A.). The aim of this work was to investigate the efficacy and dose of those commercial baits against D. reticulatum. The molluscicides (Acay, Clartex and Babotox), at doses of 3, 4 and 5 kg/ha and dry and moist soil conditions were investigated in terraria trials. The efficacy of the molluscicides and dose level was also evaluated in field experiments at two sites at Balcarce in the southeast of Buenos Aires Province, Argentina. The proportions of dead slugs did not show significant interactions between soil conditions, molluscicides and their dose (p&gt;0.05). The proportion of dead slugs, in moist conditions, was significantly lower than under dry conditions (p
THE CHANGING AGE INCIDENCE OF JODBASEDOW IN TASMANIA (1973)

Jodbasedow, as defined by Kocher (1910), described iodine-induced thyrotoxicosis affecting elderly goitrous people who lived in areas which were iodine deficient. The correction of chronic iodine deficiency in Tasmania in 1966 produced an increased incidence of thyrotoxicosis, but the age groups affected appear to be different to those described by Kocher. There was an increase in the incidence of thyrotoxicosis in middle-aged and elderly goitrous people, particularly in the first two or three years after iodation of bread, but this has been followed by a falling incidence. In contrast, there was a similar peak in the incidence of thyrotoxicosis in the younger age group which has been maintained or is rising gradually. Both increases far exceed that expected by population growth, and their sum, representing the total incidence, may remain at a steady state indefinitely.
Figure S7 from mDKN-01, a Novel Anti-DKK1 mAb, Enhances Innate Immune Responses in the Tumor Microenvironment (2023)

&lt;p&gt;Flow Cytometry Gating Strategy&lt;/p&gt;
Substituent effects in [3,3]-sigmatropic rearrangements. Alkyl group effects and transition-state syn-diaxial interactions (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstituent effects in [3,3]-sigmatropic rearrangements. Alkyl group effects and transition-state syn-diaxial interactionsCraig S. Wilcox and Robert E. BabstonCite this: J. Am. Chem. Soc. 1986, 108, 21, 6636–6642Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://doi.org/10.1021/ja00281a031RIGHTS & PERMISSIONSArticle Views451Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (885 KB) Get e-Alertsclose Get e-Alerts
The effect of physical activity on health-related quality of life in older people living in residential care settings: preliminary results from an italian sample (2023)

Population Medicine considers the following types of articles:• Research Papers -reports of data from original research or secondary dataset analyses.• Review Papers -comprehensive, authoritative, reviews within the journal's scope.These include both systematic reviews and narrative reviews.• Short Reports -brief reports of data from original research.• Policy Case Studies -brief articles on policy development at a regional or national level.• Study Protocols -articles describing a research protocol of a study.• Methodology Papers -papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation.• Methodology Papers -papers that present different methodological approaches that can be used to investigate problems in a relevant scientific field and to encourage innovation.
Transcriptomically-guided mesendoderm induction of human pluripotent stem cells using a systematically defined culture scheme (2019)

Summary Human pluripotent stem cells (hPSCs) are an essential cell source in tissue engineering, studies of development, and disease modeling. Efficient, broadly amenable protocols for rapid lineage induction of hPSCs are of great interest in the stem cell biology field. We describe a simple, robust method for differentiation of hPSCs into mesendoderm in defined conditions utilizing single-cell seeding (SCS) and BMP4 and Activin A (BA) treatment. Gene sets and gene ontology terms related to mesoderm and endoderm differentiation were enriched after 48 hours of BA treatment. BA treatment was readily incorporated into existing protocols for chondrogenic and endothelial progenitor cell differentiation. After prolonged differentiation in vitro or in vivo, BA pre-treatment resulted in higher mesoderm and endoderm levels at the expense of ectoderm formation. These data demonstrate that SCS with BA treatment is a powerful method for induction of mesendoderm that can be integrated into protocols for mesoderm and endoderm differentiation.
Antimicrobial susceptibility of Pseudomonas aeruginosa: results of a UK survey and evaluation of the British Society for Antimicrobial Chemotherapy disc susceptibility test (2001)

A survey was conducted in 1999, first to establish the prevalence of antibiotic resistance among clinical isolates of Pseudomonas aeruginosa in the UK and secondly to test whether the use of the standardized British Society for Antimicrobial Chemotherapy (BSAC) disc testing method improved the accuracy of routine susceptibility testing for this organism. Twenty-five hospitals were each asked to collect up to 100 consecutive, clinically significant isolates of P. aeruginosa and to test their susceptibility to amikacin, gentamicin, ceftazidime, imipenem, meropenem, ciprofloxacin, piperacillin and piperacillin/tazobactam using the new BSAC disc method. A total of 2194 isolate reports were available for analysis and 10% of the isolates represented, plus those with unusual resistances, were re-tested centrally for quality control purposes. The zone distributions were essentially unimodal, indicating the absence of major populations with acquired resistance. The results indicated that resistance rates to the beta-lactam, aminoglycoside and quinolone agents tested in P. aeruginosa in the UK remain low (<12%), and were mostly unchanged since a previous survey conducted in 1993. High resistance rates were nevertheless reported for isolates from cystic fibrosis patients. The accuracy of susceptibility testing using the new BSAC disc testing method was better than in previous studies, when Stokes' method was most frequently used. Critically, the proportion of resistant isolates incorrectly reported as susceptible was reduced significantly; nevertheless, depending on the antibiotic, up to 49% of the isolates reported as intermediate or resistant were found susceptible on central re-testing.
Juvenile granulomatous cutaneous T cell lymphoma: a new entity in cutaneous lymphomas? (1997)

17th Colloquium of the International Society of Dermatopathology (ISD) and Swiss Group of Dermatopathology (SGDP) of the Swiss Society of Dermatology and Venerology
WIDE BANDGAP CUfGA,IN)SE2/(ZN.CD)S HETEROJUNCTIONS (1988)

Thin film heterojunctions consisting of vacuum evaporated Cu(Ga,In)Se2 absorber and (Zn,Cd)S window layers were prepared. Efficiencies between 7.8% at 30% Ga and 6.7% at 70% Ga have been obtained. The open circuit voltage increases according to the bandgap. Still reasonable properties are obtained even for large deviations from stoichiometry to the Ga,In-rich side of the phase diagram in comparison to CuInSe2. Cells with high Ga content deteriorate during air anneal and improve with reducing treatments. Measurements of capacitance, jV-characteristic and quantum efficiency indicate that the space charge region is shifted into the window by annealing in air. Since the electrical field in the absorber decreases therefore less photogenerated carriers are collected.
An Application of AHP for the Selection of Optimum Product of BWTS for over 10,000 TEU Container Ship (2015)

The Ballast Water Treatment System was developed to prevent the unintended transport of unwanted organisms from one region to another as demanded by the IMO (International Maritime Organization). Although various types of BWTS in the world have been developed until now and applied to various ships, there has been no systematic basis for its selection and installation. Currently, the system selection and installation are as per ship owner&#x2019;s suggestion or by easy installation point of view by the shipyard. In order to organize, systemize and solve problems related to the selection and installation of BWTS, a definitive study has been performed to come up with the best alternative to derive value and criteria which were to be met for vessels which are to be equipped with BWTS. Multiple criteria were compared alongside each other during the course of this study. Accordingly an AHP (Analytic Hierarchy Process) analysis method for A, B and C companies were done for container ships with size 10,000 TEU and above. Equipment type for &#x201C;A&#x201D; company is &#x201C;Filter, UV & TiO2&#x201D; combined type. For &#x201C;B&#x201D; company it is &#x201C;Filter & UV&#x201D; combined type. Finally for &#x201C;C&#x201D; company it is &#x201C;Electrolysis&#x201D; type. Henceforth, the results of this study aims to come up with the optimum way to select the best and the most suitable BWTS for a certain vessel.
Relative turnover of (/sup 3/H)arachidonic acid and (/sup 14/C)eicosapentaenoic acid in stimulated human platelets (1986)

The relative release of arachidonic acid (AA) versus eicosapentaenoic acid (EPA) from platelet phospholipids may be important in accounting for the potential of dietary fish oil containing EPA to alter platelet reactivity. Human platelets enriched in EPA and prelabelled with (/sup 3/H)AA and (/sup 14/C)EPA were used to examine the relative losses of these fatty acids from platelet phospholipids upon stimulation. Washed dual-labelled platelets were incubated with and without thrombin in the presence of BW755C and in the presence and absence of trifluoperazine. The platelet lipids were extracted and the individual phospholipids as well as diacylglycerol (DG), phosphatidic acid (PA), etc. were separated by thin-layer chromatography and the radioactivity in each fraction determined. The (/sup 3/H)AA/(/sup 14/C)EPA dpm ratio for the loss in radioactivity from PC upon thrombin stimulation was similar to that for the PC in resting platelets. This suggests no marked selectivity in the degradation of AA versus EPA species of PC during platelet activation. The (/sup 3/H)/(/sup 14/C) ratios for the increased radioactivity in DG and PA upon thrombin stimulation were slightly higher than the ratio in PI from resting platelets suggesting only a minor preference for 1-acyl 2-arachidonoyl PI over 1-acyl 2-eicosapentaenoyl PI in the pathwaymore » from PI to DG to PA.« less
In situ powder X-ray diffraction study of the process of NiMoO4–SiO2 reduction with hydrogen (2016)

An interest in NiMoО4–SiO2 reduction stems from its promising use as catalysts for hydrodeoxygenation and hydrodesulfurization processes. The work exploits in situ X-ray diffraction to investigate phase transformations during NiMoO4–SiO2 reduction with hydrogen in a temperature range of 30-700°C. The α-NiMoО4 reduction is shown to proceed in two stages. In the first stage, at 400-500°C, an intermediate state (Ni,Mo,□)O mixed oxide and Ni1–x Mo x form. In the second stage, above 650°C, two solid solutions based on the Mo and Ni structures form. The structure of the intermediate state is refined by the Rietveld method. It is demonstrated that the Ni–Mo mixed oxide forms based on the NiO structure, which contains a certain number of cation vacancies.
Space object, high-resolution, optical imaging simulation of space-based systems (2012)

Acquiring optical images of space objects is one of the most important goals of space-based optical surveillance systems. However, it's actually difficult to obtain enough high resolution optical images for space object recognition, attitude measurement and situational awareness. To solve this problem, the imaging model of space-based optical camera and the imaging characteristics of space objects are analyzed in this paper, and a novel method of image simulation is proposed. The high resolution images of space objects simulated by our method are visually similar to the actual imaging results and may provide data support for further research on space technology.
Rapid Esterification of Nucleosides to Solid-Phase Supports for Oligonucleotide Synthesis Using Uronium and Phosphonium Coupling Reagents (1999)

Nucleosides can be esterified to solid-phase supports using uronium or phosphonium coupling reagents and a coupling additive, such as 1-hydroxybenzotriazole (HOBT), 7-aza-1-hydroxybenzotriazole (HOAT), N-methylimidazole (NMI), or 4-(dimethylamino)pyridine (DMAP). However, DMAP was far superior to other additives and high nucleoside loadings (up to 60 micromol/g) and rapid coupling reactions (< or = 10 min) were possible. Hydroxyl-derivatized CPG was attached to nucleosides with 3'-succinyl or 3'-hydroquinone-O, O'-diacetic acid (HQDA or Q-Linker) carboxyl groups through a primary ester linkage. Alternatively, supports derivatized with succinic acid or the Q-Linker were attached directly to the 3'-OH group of nucleosides through a secondary ester linkage. Uronium reagents (HATU or HBTU) gave the best results with the HQDA linker arm, while the bromophosphonium (BrOP or PyBrOP) reagents were best with the succinyl linker arm. In all cases, the coupling reactions were much faster than previous methods using carbodiimide coupling reagents. The ease and speed of the reaction make this support derivatization procedure suitable for automated in situ couplings on DNA synthesizers.
Real-time baggage tracking using a modified background subtraction algorithm (2012)

This paper describes preliminary work in designing a machine vision based airport baggage tracking system. A simplified codebook based background subtraction method is used to segment the bag. Morphological processing using an integral image is used to filter the foreground mask and the bag location is found using statistical methods. The system was implemented on a cost effective embedded processor and runs in real time at 30fps.
Silicon waveguide polarization rotator sampled Bragg grating (2017)

In this Letter, we demonstrate, to the best of our knowledge, the first experimental results of sampled Bragg grating with polarization rotator function. Silicon waveguide is used. Multiple polarization-independent reflection wavelength peaks were obtained.
Design and assessment of species-level qPCR primers targeting comammox (2018)

Abstract Published PCR primers targeting the ammonia monooxygenase gene ( amoA ) were applied to samples from activated sludge systems operated with low dissolved oxygen (DO) to quantify total and clade-level Nitrospira that perform complete ammonium oxidation (comammox); however, we found these existing primers resulted in significant artifact-associated non-target amplification. This not only overestimated comammox amoA copies but also resulted in numerous false positive detections in the environmental samples tested, as confirmed by gel electrophoresis. Therefore, to more accurately quantify known comammox, we designed specific and sensitive primers targeting three candidate species: Candidatus ( Ca. ) Nitrospira nitrosa, Ca. N. inopinata, and Ca. N. nitrificans. The primers were tested with amoA templates of these candidate species, and used to quantify comammox at the species level in low DO activated sludge systems. We found that comammox related to Ca. N. nitrosa were present and abundant in the majority of samples from low DO bioreactors and were not detected in samples from a high DO system. In addition, the greatest abundance of Ca. N. nitrosa was found in bioreactors operated with a long solids retention time. Ca. N. inopinata and Ca. N. nitrificans were only detected sporadically in these samples, indicating a minor role of these comammox in nitrification under low DO conditions. Abstract art
Telomere Attrition in Human Lens Epithelial Cells Associated with Oxidative Stress Provide a New Therapeutic Target for the Treatment, Dissolving and Prevention of Cataract with N-Acetylcarnosine Lubricant Eye Drops. Kinetic, Pharmacological and Activity-Dependent Separation of Therapeutic Targeting: Transcorneal Penetration and Delivery of L-Carnosine in the Aqueous Humor and Hormone-Like Hypothalamic Antiaging Effects of the Instilled Ophthalmic Drug Through a Safe Eye Medication Technique (2016)

The research utilizing the N-acetylcarnosine lubricant eye drops powerful therapeutic platform provides the findings related to the intraocular uptake exposure sources as well as a timing dosage and duration systemic absorption of said preparation from the conjunctional sac reaching the hypothalamus with activities transfer into the hypothalamic-neuroendocrine pathways affecting across the hypothalamus metabolic pathway the telomere biology and cataract disease occurrence, reversal and prevention and the average expected lifespan of an individual. Such findings can be translated into clinical practice and may provide a basis for personalized cataract disease and aging prevention and treatment approaches.
Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b (1979)

Eleven independent insertion mutations were isolated that prevented expression of major outer membrane protein 1b. Seven of the mutations were Mucts insertions located at ombP. These ompB::Mucts strains fell into two phenotypic classes with regard to expression of proteins 1a and 1b. The remaining four mutants were comprised of one Tn5 and three Mucts insertions mapping at par. The Mucts insertions at par were used to construct fusions of the lac operon to the par promoter. Expression of beta-galactosidase in these fusion strains reflected known regulatory properties of protein 1b. When an ompB allele was introduced into the par-lac fusion strains, beta-galactosidase activity was reduced 14- to 31-fold. Transcriptional regulation of the par gene and the existence of two functions at ompB are discussed. The results suggest that par is the structural gene for protein 1b and that an ompB gene product is a diffusible, positive regulatory element controlling expression of par.