gebhart/patrepeval huggingface dataset:
FICD activity and AMPylation remodelling modulate human neurogenesis (2020)

Abstract Posttranslational modification (PTM) of proteins represents an important cellular mechanism for controlling diverse functions such as signalling, localisation or protein–protein interactions. AMPylation (also termed adenylylation) has recently been discovered as a prevalent PTM for regulating protein activity. In human cells AMPylation has been exclusively studied with the FICD protein. Here we investigate the role of AMPylation in human neurogenesis by introducing a cell-permeable propargyl adenosine pronucleotide probe to infiltrate cellular AMPylation pathways and report distinct modifications in intact cancer cell lines, human-derived stem cells, neural progenitor cells (NPCs), neurons and cerebral organoids (COs) via LC–MS/MS as well as imaging methods. A total of 162 AMP modified proteins were identified. FICD-dependent AMPylation remodelling accelerates differentiation of neural progenitor cells into mature neurons in COs, demonstrating a so far unknown trigger of human neurogenesis.
Flow Cytometry: Principles and Clinical Applications in Hematology (2000)

The use of flow cytometry in the clinical laboratory has grown substantially in the past decade. This is attributable in part to the development of smaller, user-friendly, less-expensive instruments and a continuous increase in the number of clinical applications. Flow cytometry measures multiple characteristics of individual particles flowing in single file in a stream of fluid. Light scattering at different angles can distinguish differences in size and internal complexity, whereas light emitted from fluorescently labeled antibodies can identify a wide array of cell surface and cytoplasmic antigens. This approach makes flow cytometry a powerful tool for detailed analysis of complex populations in a short period of time. This report reviews the general principles in flow cytometry and selected applications of flow cytometry in the clinical hematology laboratory.
Treatment of Three- and Four-Part Fractures of the Proximal Humerus with a Modified Cloverleaf Plate (1994)

Twenty-six patients, ranging in age from 19 to 62 years (average 55), were evaluated an average of 6 years and 2 months (range 1-12 years) after open reduction and internal fixation of a three- or four-part fracture of the proximal humerus using a modified cloverleaf plate. Six patients had an associated dislocation of the humeral head. All fractures healed. No avascular necrosis of the humeral head was observed at follow-up. Twenty-two patients had an excellent result, two a good result, and two a fair result. The modified cloverleaf plate proved to be an excellent method for the treatment of proximal humerus fractures. Open reduction and internal fixation of three- and four-part fractures of the proximal humerus should be considered as initial treatment by an experienced surgeon, oven before use of a prosthetic replacement, which is recommended only for elderly patients with osteopenia.
Measurement of Mitochondrial Respiration ‐ Comparison of Two Respirometers: MitoCellMt200A versus O2k (2021)

Background Mitochondrial function is a critical determinant of cardiomyocyte survival after ischemia/reperfusion (I/R) and thus an important target of cardioprotection. A reliable measurement of mitochondrial respiration is therefore essential for cardioprotection research. Methods Mitochondria were isolated by differential centrifugation from naïve rat hearts (n=8‐14) or isolated buffer‐perfused rat hearts subjected to 30 min global zero‐flow ischemia and 10 min reperfusion (n=8‐14). Mitochondrial respiration was measured in 100 µg/ml mitochondria at 37°C during magnetic stirring using two different Clark‐type electrode respirometers, the MitoCellMt200A from Strathkelvin (Glasgow, UK) and the O2k from Oroboros (Innsbruck, AT). Two different respiration buffers (incubation buffer and MiR05 buffer) were used as recommended: in mmol/L; incubation buffer: 125.0 KCl, 10.0 3‐(N‐morpholino) propane sulfonoic acid, 2.0 MgCl 2 , 5.0 KH 2 PO 4 , and 0.2 ethylene glycol tetra‐acetic acid; MiR05 buffer: 0.5 ethylene glycol tetra‐acetic acid, 3.0 MgCl 2 , 60.0 lactobionic acid, 20.0 taurine, 10.0 KH 2 PO 4 , 20.0 4‐(2‐hydroxyethyl)‐1‐piperazine ethane sulfonic acid, and 110.0 D‐sucrose. Glutamate and malate (5.0 mmol/L, each) were added as complex I substrates. Complex I respiration was measured after adding adenosine diphosphate (ADP, 1.0 mmol/L). Complex IV respiration was stimulated by adding N,N,N′,N′‐tetra‐methyl‐p‐phenylene diamine (TMPD, 300 µmol/L) and ascorbate (3 mmol/L). Mitochondrial respiration was normalized to mitochondrial protein content. Results Baseline, ADP‐stimulated complex I and complex IV respiration were higher in mitochondria isolated from naïve rat hearts than in those isolated from rat hearts, which had undergone global zero‐flow ischemia and reperfusion ‐ independently of the used respirometers and the used respiration buffers (see Table 1). Baseline respiration was l higher in the MitoCellMt200A respirometer ‐ independently of the respiration buffer used (see Table 1). ADP‐stimulated complex I respiration was comparable between the respirometers, whereas the MiR05 buffer resulted in higher respiration than the incubation buffer (see Table 1). Complex IV respiration, as an indicator for equal loading of the chambers with viable mitochondria, was comparable between the respirometers and the buffers but, as expected, reduced after ischemia/reperfusion (see Table 1). Conclusion Independently of the Clark‐type electrode respirometers or respiration buffers used, we identify a reduction of mitochondrial respiration after myocardial I/R. However, the respiration buffer composition determines the magnitude of mitochondrial ADP‐stimulated complex I respiration. A lower sensitivity of the MitoCellMt200A respirometer in comparison to the O2k high‐resolution respirometer at low oxygen consumption may affect the accuracy of baseline respiration measurements.
Traveling Charge Computations - Experimental Comparisons and Sensitivity Studies, (1987)

Abstract : Theoretical performance studies were conducted for 14 mm gun operated in the conventional and traveling charge (TC) modes using the XKTC interior ballistic code. Conventional charge calculations, done by way of model validation, agreed closely with experimental firings. Traveling charge calculations were done to predict the effects of varying parameters such as TC ignition time and burning behavior on charge performance. For a propelling charge composed of a conventional booster propellant and a TC element, delaying TC ignition past maximum pressure from the booster predicts the greatest improvement in TC performance. TC performance appeared to be less sensitive to the details of burning rate pressure dependence than to overall TC element burn time. Uncontrolled burning characterized by very short burn times leads to excessive gun pressures.
Dynamically Optimized Human Eyes-to-Face Generation via Attribute Vocabulary (2023)

Generating face from human eyes, named eyes-to-face generation, is an interesting research topic of face synthesis, which has great potential in the field of public security. One of the main challenges in eyes-to-face generation is the unbalanced information between inputs and outputs, where the outputs are complete facial images while the inputs only contain limited information in the region of eyes. The existing methods generate faces directly from eyes without considering the possibly available facial information (e.g. facial attributes), resulting in inaccurate predictions and high uncertainty in those features less correlated with eyes (e.g. hairstyle, moustache, facial contour). To address this challenge, we propose a two-stage solution (named EA2F-GAN) to dynamically optimize eyes-to-face generation via attribute vocabulary. In addition, a dataset named TEAF is constructed based on the public datasets CelebA and LFW, containing 138,934 triples of eye image, attribute vocabulary, and face image. Sufficient experimental results show that, by incorporating additional facial attributes, our proposed approach can synthesize realistic face with high consistency to the original one, significantly overwhelming state-of-the-art methods.
d-Glucose Oxidation by Cold Atmospheric Plasma-Induced Reactive Species (2022)

The glucose oxidation cascade is fascinating; although oxidation products have high economic value, they can manipulate the biological activity through posttranslational modification such as glycosylation of proteins, lipids, and nucleic acids. The concept of this work is based on the ability of reactive species induced by cold atmospheric plasma (CAP) in aqueous liquids and the corresponding gas-liquid interface to oxidize biomolecules under ambient conditions. Here, we report the oxidation of glucose by an argon-based dielectric barrier discharge plasma jet (kINPen) with a special emphasis on examining the reaction pathway to pinpoint the most prominent reactive species engaged in the observed oxidative transformation. Employing d-glucose and d-glucose-<sup>13</sup>C<sub>6</sub> solutions and high-resolution mass spectrometry and ESI-tandem MS/MS spectrometry techniques, the occurrence of glucose oxidation products, for example, aldonic acids and aldaric acids, glucono- and glucaro-lactones, as well as less abundant sugar acids including ribonic acid, arabinuronic acid, oxoadipic acid, 3-deoxy-ribose, glutaconic acid, and glucic acid were surveyed. The findings provide deep insights into CAP chemistry, reflecting a switch of reactive species generation with the feed gas modulation (Ar or Ar/O<sub>2</sub> with N<sub>2</sub> curtain gas). Depending on the gas phase composition, a combination of oxygen-derived short-lived hydroxyl (<sup>•</sup>OH)/atomic oxygen [O(<sup>3</sup>P)] radicals was found responsible for the glucose oxidation cascade. The results further illustrate that the presence of carbohydrates in cell culture media, gel formulations (agar), or other liquid targets (juices) modulate the availability of CAP-generated species <i>in vitro</i>. In addition, a glycocalyx is attached to many mammalian proteins, which is essential for the respective physiologic role. It might be questioned if its oxidation plays a role in CAP activity.
Diethyl Azelate for the Treatment of Brown Recluse Spider Bite, a Neglected Orphan Indication (2021)

DEA is a promising novel therapy for the brown recluse spider bite and perhaps even unrelated envenomations involving PLDs.
Soft-Output Detection Of Cpm Signals Transmitted Over Channels Affected By Phase Noise (2006)

Publication in the conference proceedings of EUSIPCO, Florence, Italy, 2006
Petrophysical evaluation of subterranean formations (2023)

Methods and systems are provided for evaluating petrophysical properties of subterranean formations and comprehensively evaluating hydrate presence through a combination of computer-implemented log modeling and analysis. Certain embodiments include the steps of running a number of logging tools in a wellbore to obtain a variety of wellbore data and logs, and evaluating and modeling the log data to ascertain various petrophysical properties. Examples of suitable logging techniques that may be used in combination with the present invention include, but are not limited to, sonic logs, electrical resistivity logs, gamma ray logs, neutron porosity logs, density logs, NRM logs, or any combination or subset thereof.
Discovery of New Cargo Proteins that Enter Cells through Clathrin‐Independent Endocytosis (2009)

Clathrin-independent endocytosis (CIE) allows internalization of plasma membrane proteins lacking clathrin-targeting sequences, such as the major histocompatibility complex class I protein (MHCI), into cells. After internalization, vesicles containing MHCI fuse with transferrin-containing endosomes generated from clathrin-dependent endocytosis. In HeLa cells, MHCI is subsequently routed to late endosomes or recycled back out to the plasma membrane (PM) in distinctive tubular carriers. Arf6 is associated with endosomal membranes carrying CIE cargo and expression of an active form of Arf6 leads to the generation of vacuolar structures that trap CIE cargo immediately after endocytosis, blocking the convergence with transferrin-containing endosomes. We isolated these trapped vacuolar structures and analyzed their protein composition by mass spectrometry. Here we identify and validate six new endogenous cargo proteins (CD44, CD55, CD98, CD147, Glut1, and ICAM1) that use CIE to enter cells. CD55 and Glut1 appear to closely parallel the trafficking of MHCI, merging with transferrin endosomes before entering the recycling tubules. In contrast, CD44, CD98, and CD147 appear to directly enter the recycling tubules and by-pass the merge with EEA1-positive, transferrin-containing endosomes. This divergent itinerary suggests that sorting may occur along this CIE pathway. Furthermore, the identification of new cargo proteins will assist others studying CIE in different cell types and tissues.
Efficacy and safety of high concentration lidocaine for trigeminal nerve block in patients with trigeminal neuralgia (2007)

Trigeminal nerve block with high concentration lidocaine (10%) is capable of achieving an intermediate period of pain relief, particularly in patients with lower pain intensity and shorter pain duration prior to the procedure.
ChemInform Abstract: Synthesis and Anti‐HIV‐1 Activity of Novel Bicyclic Nucleoside Analogues Restricted to an S‐Type Conformation. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Root Weevil (Coleoptera: Curculionidae) and Ground Beetle (Coleoptera: Carabidae) Immigration into Strawberry Plots Protected by Fence or Portable Trench Barriers (2005)

Physical exclusion shows some potential as a novel root weevil control strategy, but barriers to root weevil immigration may also exclude beneficial insects, such as ground beetles. A field study was undertaken in 1997 to assess the impact of two physical barriers—portable plastic trenches and aluminum fences with Teflon tape—on root weevil and ground beetle immigration into plots of strawberry, Fragaria x ananassa (Duchesne). Barypeithes pellucidus (Boheman) and Nemocestes incomptus (Horn), each comprised 43% of the root weevils caught at the site. Most (86%) of the ground beetles caught in control plots were longer than 1 cm, the width of the gap in the portable trench top. Trenches excluded 75 and 63% of B. pellucidus and N. incomptus, respectively, without significantly reducing immigration of large (<1 cm) ground beetles. Fences excluded 65, 84, and 99% of B. pellucidus, N. incomptus, and large ground beetles, respectively. Adding diatomaceous earth to trenches did not increase their efficacy, and fences without Teflon tape excluded ground beetles but not root weevils. The reduction in the population of root weevils and other strawberry pests caused by the use of barriers reduced damage to strawberry plant leaves and increased strawberry plant survival relative to unprotected control plots. Advantages and disadvantages of these physical control tools are discussed with a view to creating superior tools for root weevil exclusion, compatible with an integrated pest management approach. Portable trenches may offer a means of selectively excluding root weevils but not ground beetles.
Large-Scale Micromagnetics Simulation of Magnetization Dynamics in a Permanent Magnet during the Initial Magnetization Process (2019)

For better motors in electric vehicles, we had better understand magnetism at the microscale. Domain-wall motions and magnetization-reversal processes during the initial magnetization process in a hot-deformed Nd${}_{2}$Fe${}_{14}$B permanent magnet are simulated by solving the Landau-Lifshitz-Gilbert equation for a model ensemble of 5302 grains. The initial-magnetization curve reproduces the two-step structure seen in experiment. In the first step, the domain walls move inside the multidomain grains, and the magnetization reversal of the single domains occurs in the second step. The effects of networks among the grains through dipolar interaction are also estimated.
Low-profile, high-permeability antennaless RFID tags for use on metal objects (2012)

Radio-frequency identification (RFID) systems are used in asset tracking, but general-purpose tags typically do not perform well on or near metal. Therefore, custom solutions for on metal applications are common. Many solutions for on-metal tags use spacers or electromagnetic band-gap structures resulting in designs that are extremely thick. Our approach was to incorporate materials with permeabilities greater than 1 to create an impedance across a metal surface, diverting current into the tag IC. This type of tag is nearly antennaless as it uses the ground plane or container to excite currents through the IC. Our research has shown that these tags are sensitive to several parameters, such as the size and shape of the plane upon which they are placed, the material used in the antenna, and the type of matching network being used. Tags using magnetic materials in this manner are significantly thinner than those developed using other methods.
Synthesis of Axially Chiral 1,1′‐Binaphthalenes by Palladium‐Catalysed Cross‐Coupling Reactions of Triorganoindium Reagents (2013)

Abstract 1,1′‐Binaphthalenes and heterocyclic analogues can be efficiently prepared by palladium‐catalysed cross‐coupling reactions between tri(1‐naphthyl)indium reagents and 1‐halonaphthalenes and haloisoquinolines. The reactions were usually carried out in THF at 80 °C with a slight excess of the indium reagent (40 mol‐%) and a low catalyst loading (4 mol‐% Pd) to afford the cross‐coupling products in good yields (45–99 %). The method allows the synthesis of sterically hindered 2‐substituted and 2,2′‐disubstituted 1,1′‐binaphthalenes and naphthylisoquinolines. In addition, the coupling reactions can be performed enantioselectively and the best enantiomeric excesses were obtained by using the chiral amino‐phosphane ferrocenyl ligand ( R , S )‐PPFA.
Siliceous Rhizaria abundances and diversity in the Mediterranean Sea assessed by combined imaging and metabarcoding approaches (2022)

Siliceous Rhizaria (polycystine radiolarians and phaeodarians) are significant contributors to carbon and silicon biogeochemical cycles. Considering their broad taxonomic diversity and their wide size range (from a few micrometres up to several millimetres), a comprehensive evaluation of the entire community to carbon and silicon cycles is challenging. Here, we assess the diversity and contribution of silicified Rhizaria to the global biogenic silica stocks in the upper 500 m of the oligotrophic North-Western Mediterranean Sea using both imaging (FlowCAM, Zooscan and Underwater Vision Profiler) and molecular tools and data. While imaging data (cells m -3 ) revealed that the most abundant organisms were the smallest, molecular results (number of reads) showed that the largest Rhizaria had the highest relative abundances. While this seems contradictory, relative abundance data obtained with molecular methods appear to be closer to the total biovolume data than to the total abundance data of the organisms. This result reflects a potential link between gene copies number and the volume of a given cell allowing reconciling molecular and imaging data. Using abundance data from imaging methods we estimate that siliceous Rhizaria accounted for up to 6% of the total biogenic silica biomass of the siliceous planktonic community in the upper 500m of the water column.
Agranulocytosis secondary to zinc excess: Clinically relevant observations, including response to G‐CSF and oral copper (2020)

Anemia and leukopenia because of copper deficiency can be mistaken for myelodysplasia. Key issues, including response to G-CSF and oral copper, are discussed. This case illustrates a significant deleterious effect of excessive zinc consumption.
Les Fondements Cybernetiques de L'Activite Nerveuse.-- (1972)

This book is written in French for the neurologist unfamiliar with the mathematical language of cybernetics. The authors survey the contributions of computer models to our understanding of a number of neurological processes, from parkinsonian tremor to freudian psychopathology. The book is highly readable and easy to understand, even for the novitiate. Surprisingly, the cerebellum receives only scant attention. The book's lack of a bibliography greatly decreases its usefulness.
Pathology of “Berkeley” sickle-cell mice includes gallstones and priapism (2006)

![Figure][1] 

Pigment gallstones in “Berkeley” transgenic sickle cell mice. HE original magnification, × 12.5. (B) Stones in a hyperplastic gallbladder; original magnification, × 40.



We appreciate the histopathologic survey of “Berkeley”
Evaluation of Biocompatibility and Administration Site Reactogenicity of Polyanhydride‐Particle‐Based Platform for Vaccine Delivery (2012)

Efficacy, purity, safety, and potency are important attributes of vaccines. Polyanhydride particles represent a novel class of vaccine adjuvants and delivery platforms that have demonstrated the ability to enhance the stability of protein antigens as well as elicit protective immunity against bacterial pathogens. This work aims to elucidate the biocompatibility, inflammatory reactions, and particle effects on mice injected with a 5 mg dose of polyanhydride nanoparticles via common parenteral routes (subcutaneous and intramuscular). Independent of polymer chemistry, nanoparticles more effectively disseminated away from the injection site as compared to microparticles, which exhibited a depot effect. Using fluorescent probes, the in vivo distribution of three formulations of nanoparticles, following subcutaneous administration, indicated migration away from the injection site. Less inflammation was observed at the injection sites of mice-administered nanoparticles as compared to Alum and incomplete Freund's adjuvant. Furthermore, histological evaluation revealed minimal adverse injection site reactions and minimal toxicological effects associated with the administration of nanoparticles at 30 days post-administration. Collectively, these results demonstrate that polyanhydride nanoparticles do not induce inflammation as a cumulative effect of particle persistence or degradation and are, therefore, a viable candidate for a vaccine delivery platform.
Technologies for high-speed and power-efficient routers and switches (2008)

We worked on the physical layer model for implementing the optical DQPSK transmission function. In order to enable a high speed DQPSK transmission, a signal multiplexing and de-multiplexing module was needed. The multiplexing and de-multiplexing module exchanges a high frequency 2bit width data signal and a low frequency wide width data signal into each other. For creating such modules, the channel lengths of each low frequency signal must be kept even. We also worked on creating an evaluation board which consists a 64B/66B encoder and decoder, a gearbox, forward-error-correction (FEC) units, and the deskewing units by using two FPGApsilas. By connecting four 10-Gbps Multiplexer/Demultiplexer LSIs to this board, a transmission rate of 40 Gbps was achieved. As a different approach, we placed Dynamic Performance Control that cuts down the power consumption according to the received traffic, as required technologies for future routers and switches. Combining the two technologies together, a high-speed and power efficient routers and switches are achievable.