gebhart/patrepeval huggingface dataset:
Isolation of a candidate gene, CAB1, for cholesterol transport to mitochondria from the c-ERBB-2 amplicon by a modified cDNA selection method. (1997)

An improved cDNA selection method was established to isolate expressed genes efficiently from an amplified chromosome region in human cancer. Biotinylated yeast artificial chromosome DNA containing c-ERBB-2 was hybridized in solution with PCR-amplifiable cDNAs of an esophageal cancer cell line bearing the c-ERBB-2 amplification. After capturing the hybrids on avidin-coated magnetic beads, the cDNAs were amplified by PCR. Four new genes (A39, C51, CAB1, and GRB-7) coamplified with c-ERBB-2 were isolated from the enriched cDNA library. CAB1, GRB-7, and c-ERBB-2 were overexpressed in gastric and esophageal cancer cells in correspondence with the amplification. The deduced amino acid sequence of the CAB1 gene had significant homology to the recently discovered steroidogenic acute regulatory protein, StAR, which plays an essential role in cholesterol transport to mitochondria. It was established that multiple overexpressed genes are frequently present in a single amplicon.
Analysis and identification of discrete-time systems via discrete Legendre orthogonal polynomials (1987)

A new method of using discrete Legendre orthogonal polynomials (DLOPs) is proposed for the analysis and identification of linear time invariant discrete time systems. It is based on representing the DLOP expansion vector of the delayed function y(k — 1) in terms of that of the original discrete function y(k). Several examples are worked out to illustrate the utility of the method.
DCLK1 integrates induction of TRIB3, EMT, drug resistance and poor prognosis in colorectal cancer (2019)

Doublecortin-like kinase 1 (DCLK1) promotes tumour proliferation in human colorectal cancer (CRC). To elucidate the mechanism and clinical relevance of this association, we performed expression analysis using commercially available colon carcinoma cell lines (SW480, HCT116, CaCO2, SW48 and SKCO1) and immunohistochemical analysis of 200 resected CRC samples for correlation with clinical features. DCLK1 showed a high level of expression, especially in SW480 and HCT116 cells. Silencing DCLK1 expression using short hairpin DCLK1 (shDCLK1) RNA inhibited the growth and invasion capacities of these cell lines, which showed signs of entering into the mesenchymal-epithelial transition (MET). We found evidence of a strong correlation of DCLK1 expression with that of Tribbles homolog 3 (TRIB3), and silencing TRIB3 also led to the MET phenotype in these cells. In the clinical samples, compared with samples showing low expression of DCLK1, high expression was associated with poor prognosis in terms of overall and recurrence-free survival (P < 0.0001). The results of univariate and multivariate analysis suggested that high expression of DCLK1 in clinical colon cancer samples was tied to poor prognosis, cancer invasion depth and lymph node metastasis. DCLK1 expression correlates with malignant grade of colon cancer and offers a potential treatment target.
Modeling and Coordinate Control of Circulating Currents in Parallel Three-Phase Boost Rectifiers (2007)

In this paper, definition of the circulating currents of multiphase paralleled converters is first presented, and the circulating-current-generating mechanism is clearly explained. Thus, based on this definition, an averaged model of the circulating current is proposed. It is seen from this model that the circulating current consists of not only the zero-sequence but also the nonzero-sequence components. The governing differential equation also shows explicitly the relation between the circulating currents and the affecting factors such as different pulse width-modulation strategies. With this understanding, a simple coordinate control is then presented to reduce the circulating current. The phenomenon of the intrinsic circulating current is also explained. Furthermore, a prototype system is constructed, and the proposed control is implemented using TMS320F2812 DSP. Both simulation and experimental results verify the validity of the proposed theory and control
Magnetic materials-based electrorheological fluids (1997)

A type of electrorheological (ER) system, denoted the magnetic materials-based electrorheological fluids, is introduced. The solid particles of this system are 40–50 μ-microspheres obtained by the sol–gel processing of a ferroelectric material containing a ferromagnetic component. Since the solid material is magnetic, the presence of a small magnetic field, such as that from a small permanent magnet, can suspend the microspheres in liquid. The incorporation of a small amount of magnetic materials thereby solves the long standing problem of particle sedimentation in ER fluids. It is found that this type of ER fluid is very stable and exhibits a strong ER effect at low electric field.
Role of SNX16 in the Dynamics of Tubulo-Cisternal Membrane Domains of Late Endosomes (2011)

In this paper, we report that the PX domain-containing protein SNX16, a member of the sorting nexin family, is associated with late endosome membranes. We find that SNX16 is selectively enriched on tubulo-cisternal elements of this membrane system, whose highly dynamic properties and formation depend on intact microtubules. By contrast, SNX16 was not found on vacuolar elements that typically contain LBPA, and thus presumably correspond to multivesicular endosomes. We conclude that SNX16, together with its partner phosphoinositide, define a highly dynamic subset of late endosomal membranes, supporting the notion that late endosomes are organized in distinct morphological and functional regions. Our data also indicate that SNX16 is involved in tubule formation and cholesterol transport as well as trafficking of the tetraspanin CD81, suggesting that the protein plays a role in the regulation of late endosome membrane dynamics.
Chemical Bonding of AlH3 Hydride by Al-L2,3 Electron Energy-Loss Spectra and First-Principles Calculations (2012)

In a previous study, we used transmission electron microscopy and electron energy-loss (EEL) spectroscopy to investigate dehydrogenation of AlH₃ particles. In the present study, we systematically examine differences in the chemical bonding states of Al-containing compounds (including AlH₃) by comparing their Al-L<sub>2,3</sub> EEL spectra. The spectral chemical shift and the fine peak structure of the spectra were consistent with the degree of covalent bonding of Al. This finding will be useful for future nanoscale analysis of AlH₃ dehydrogenation toward the cell.
Improved Luminance Intensity of InGaN-GaN Light-Emitting Diode by Roughening both p-GaN and Undoped-GaN Surfaces and Applying a Mirror to the Sapphire Substrate Surface (2007)

InGaN-GaN light emitting diodes (LEDs) with double roughened surfaces and silver mirror was fabricated by surface- roughening, wafer-bonding and laser lift-off technologies. It was found the light intensity of DRM-LED (with mirror between undoped-GaN/sapphire interface) was 3.05 times higher than that of the PR-LED (with only roughened p-GaN surface), and 1.45 times higher than that of the DRSM-LED (with mirror on the backside of sapphire substrate).
Indentation Behavior of Ion‐Exchanged Glasses (1990)

Indentation fracture mechanics was extended to include the effect of a thin layer of residual stress on the identation strength of brittle materials. The proposed theory was used to predict the residual stress values for an ion‐exchanged glass. For flaws placed before the exchange, considerable strengthening was observed, but the value of the surface stress predicted was considerably underestimated. For flaws placed after the exchange, there was no strengthening and the value of the surface stress was predicted to be zero. The failure of the indentation analysis indicates that it has to be modified for accurate stress determination. Thin layers of residual stress were found to retard the initiation of surface damage, but their influence on the strength after damage initiation was minimal.
Isolation on Si wafers by MeV proton bombardment for RF integrated circuits (2001)

This paper studies issues related with using high energy protons to create local semi-insulating silicon regions on IC wafers for device isolation and realization of high-Q IC inductors. Topics on two approaches, i.e., one using Al as the radiation mask and the other using proton direct-write on wafers were studied. It was shown that Al can effectively mask the proton bombardment of 15 MeV up to the fluence of 10/sup 17/ cm/sup -2/. For the unmasking direct write of the proton bombardment, isolation in the silicon wafer can be achieved without damaging active devices if the proton fluence is kept below 1/spl times/10/sup 14/ cm/sup -2/ with the substrate resistivity level chosen at 140 /spl Omega/-cm, or kept at 1/spl times/10/sup 15/ cm/sup -2/ with the substrate resistivity level chosen at 15 /spl Omega/-cm. Under the above approaches, the 1 h-200/spl deg/C thermal treatment, which is necessary for device final packaging, still gives enough high resistivity for the semi-insulating regions while recovering somewhat the active device characteristics. For the integrated passive inductor fabricated on the surface of the silicon wafer, the proton radiation improves its Q value.
Resuscitation with lactated Ringer solution limits the expression of molecular events associated with lung injury after hemorrhage (2004)

The aim of this study was to determine whether hemorrhage altered the caspase-3 activity and the ATP levels in rat lung and ileum tissues and determine whether resuscitation with lactated Ringer solution (LR) or whole blood (WB) reversed these changes. Male Sprague-Dawley rats were briefly anesthetized with isoflurane, and their mean arterial blood pressure was reduced from 110 to 40 mmHg by bleeding. The bled rat was then resuscitated with LR or autologous WB to bring mean arterial blood pressure back to 80 mmHg. Lung and ileum tissues were removed at the end of hemorrhage or at the end of the resuscitation period for specified bioassays. Hemorrhage increased cellular caspase-3 activity in the lung and the ileum. After the hemorrhaged rats received LR or WB, caspase-3 activity returned to the basal level in the lung and ileum, respectively. Likewise, hemorrhage decreased cellular ATP levels in lung and ileum. After LR or WB resuscitation, the cellular ATP level returned to the basal level only in the lung resuscitated with LR. The increased caspase-3 activity was associated with the increased expression of caspase-3 mRNA, which also returned to normal levels after either resuscitation. Similarly, hemorrhage increased the expression of inducible nitric oxide synthase and Kruppel-like factor 6 and decreased expression of Kruppel-like factor 4. Subsequent LR resuscitation normalized the expression of these genes in the lung tissue. Our results demonstrate that resuscitation with LR can reverse the expression of genes and their products that are thought to contribute to hemorrhage-induced lung injury.
Coalescence judgment criteria for the interaction between two close surface cracks by WES2805 and its safety margin for brittle fracture assessment (2016)

It is important to consider the interaction between multiple cracks in evaluating the reliability of a structure. In this study, the stress intensity factor (<em>K</em> value) is evaluated using the finite element method for interacting surface cracks. Although there are an infinite number of possible conditions of the locations and sizes of two close cracks, the cracks shall be located parallel to each other and have the same dimensions for simplification in this study. The <em>K</em> values on the crack front are calculated under various aspect ratios and relative locations. When there is a strong interaction (Δ<em>K</em><sub>max</sub> ≥ 10%), fracture analysis is generally performed after the coalescence of the two cracks by the FFS standard. As a result of the investigation of the critical condition of the positional parameters for coalescence, judgement criteria were introduced in WES2805 with some simplification. It was revealed that the coalescence process in WES2805 provides a safety margin.
A generation/recombination model assisted with two trap centers in wide band-gap semiconductors (2013)

A generation/recombination (GR) model assisted with two trap centers has been proposed for studying reverse current on pn junctions in wide band-gap semiconductors. A level (Et1) has been assumed to be located near the bottom of the conduction band and the other (Et2) to be near the top of the valence band. The GR model has been developed by assuming (1) a high-electric field; F, (2) a short distance; d, between trap centers, (3) reduction in an energy-difference; Δeff = |Et1 – Et2| – eFd, and (4) hopping or tunneling conductions between trap centers with the same energy-level (Δeff ≈ 0). The GR rate has been modeled by trap levels, capture cross-sections, trap densities, and transition rate between trap centers. The GR rate, about 1010 greater than that estimated from the single-level model, has been predicted on pn junctions in a material with band-gap of 3.1 eV. Device simulations using the proposed GR model have been demonstrated for SiC diodes with and without a guard ring. A reasonable range for reverse current at room temperature has been simulated and stable convergence has been obtained in a numerical scheme for analyzing diodes with an electrically floating region.
[Is nutritional intervention influencing lipid allowances beneficial?]. (1992)

An earlier study (consumption study) has shown that in subjects eating normally: 1) the amount of fat consumed per day corresponds to 4/5 of the fat contained in the portions served; 2) the distribution of the fatty acids (FA) consumed is unfavorable because of the use of saturated FA for food preparation and cooking and because of the elimination of mono- and polyunsaturated FA in the unconsumed food. In order to determine whether a simple nutritional intervention might favorably modify the distribution of FA consumed, a computer simulation of a decrease in allowances of saturated FA in exchange for an increase in mono- and polyunsaturated FA was tested. The laboratory results were compared to those of the computer program. Comparison of the intervention study with the consumption study revealed that: 1) the fat content was identical; 2) there were the same number of total calories, but there were more lipidic calories consumed during the intervention (38% instead of 35%); 3) the distribution of FA more nearly approached the recommended amounts: 40% saturated FA (-25%), 39% monounsaturated FA (+8%), 21% polyunsaturated FA (+91%) and a P/S ratio of .60 (instead of .21); 4) these proportions can be explained by a decrease in palmitic (-30%) and stearic (-20%) acids and by an increase in linoleic (+100%) and linolenic (+40%) acids; 5) the allowances of elaidic acid was on the average 4.3 g per day and had little influence on the P/S ratio; 6) the omega 6/omega 3 ratio (ratio of 7) was higher than that of the consumption study (ratio of 5); 7) there was a 16% decrease in cholesterol intake (260 mg instead of 310 mg). The laboratory findings corresponded on the whole to those of the computer program that only faulted by overestimating lipid and cholesterol allowances.
Glycerol supplementation enhances the protective effect of dietary FloraMax-B11 against Salmonella Enteritidis colonization in neonate broiler chickens (2014)

Two independent trials were conducted in the present study to evaluate the effect of 5% glycerol supplementation combined with dietary FloraMax-B11 (FM) against Salmonella Enteritidis colonization in neonate broiler chickens. In each trial, 60 chicks were randomly assigned into 4 groups. Group 1 received a control diet. Group 2 received a control diet supplemented with 5% glycerol. Group 3 received a control diet supplemented with FM, and group 4 received a control diet supplemented with 5% glycerol and FM. At placement, chickens were challenged with Salmonella Enteritidis at 10(4) cfu/bird. In each trial, 12 chicks were humanely killed 72 h postchallenge, respectively, for Salmonella Enteritidis colonization. Supplementation of 5% glycerol or FM by themselves, showed no significant effect on Salmonella Enteritidis recovery or incidence when compared with control nontreated chickens in both trials. However, no detectable Salmonella Enteritidis was observed in the chickens that received the supplementation of 5% glycerol combined with FM in both trials. Further studies are in progress in older birds to substantiate these findings.
Supervised and Unsupervised Transfer Learning for Question Answering (2017)

Although transfer learning has been shown to be successful for tasks like object and speech recognition, its applicability to question answering (QA) has yet to be well-studied. In this paper, we conduct extensive experiments to investigate the transferability of knowledge learned from a source QA dataset to a target dataset using two QA models. The performance of both models on a TOEFL listening comprehension test (Tseng et al., 2016) and MCTest (Richardson et al., 2013) is significantly improved via a simple transfer learning technique from MovieQA (Tapaswi et al., 2016). In particular, one of the models achieves the state-of-the-art on all target datasets; for the TOEFL listening comprehension test, it outperforms the previous best model by 7%. Finally, we show that transfer learning is helpful even in unsupervised scenarios when correct answers for target QA dataset examples are not available.
Metal Nanoparticle Based All-Optical Photothermal Light Modulator (2014)

We present a simple scheme for the manipulation of light intensity by light mediated by a dissipative process. The implementation employs the heat released by an optically excited plasmonic metal nanoparticle to control the size of an isotropic bubble in a nematic liquid crystal film. The nematic film is designed as a zero-order half-wave plate that rotates an incident probe light polarization by π/2 and is blocked by an analyzing polarizer behind the structure. The growing isotropic bubble disturbs the half-wave plate and causes the probe to be transmitted through the modulator structure. Our results demonstrate that dissipative processes may be advantageously used to control light by light.
Audio frequency response of glass flat panel distributed mode loudspeakers (2014)

The development of glass flat panel distributed mode loudspeakers (DML’s) will enable compelling new applications, such as flat panel displays that double as loudspeakers, or in architectural and automotive applications, window glass that can produce sound or actively cancel environmental noise. In this paper we discuss the frequency response of glass flat-panel distributed mode loudspeakers. The density of panel bending modes, their quality factors (Q’s), and the modal radiation efficiencies determine a DML’s frequency response. For example, a 0.55 mm thick cover glass for a 55 in. television has more than 10 000 bending modes in the audible frequency range. The density of modes increases with frequency and above a threshold frequency, determined by the panel dimensions and the mode Q’s, the mode spacing becomes less than the width of the individual modes, so the overall frequency response approaches the smooth, flat response of an ideal pistonic loudspeaker. However, below this frequency, discrete modes produce prominent peaks in the frequency response. We present simulated and measured glass panel frequency responses, including the effect of a thin layer of trapped air behind a DML panel, and we discuss the need for high internal friction (low Q) glass.
One-Pot Procedure for the Synthesis of Unsymmetrical Diarylalkynes (2010)

Unsymmetrical diarylalkynes are accessible by a one-pot procedure from two different aryl halides and (trimethylsilyl)acetylene. The three-component coupling is initialized by a Pd/Cu-catalyzed Sonogashira coupling of an aryl halide with (trimethylsilyl)acetylene. After subsequent desilylation of the formed aryl(trimethylsilyl)acetylene with aqueous potassium hydroxide, a second Sonogashira coupling with an aryl iodide that does not require any additional Pd/Cu-catalyst gives access to an unsymmetrical diarylalkyne.
Communication—Polyethylene/PBI Pore-Filling Composite Membrane for High Performance Vanadium Redox Flow Battery (2019)

A pore-filling proton exchange membrane is fabricated by combining a porous polyethylene (PE) substrate and polybenzimidazole (PBI) resin for vanadium redox flow batteries (VRFB). The PE substrate not only optimized the structure of the composite membrane, but also significantly reduces its cost (< 30$·m−2). Compared with Nafion212 and pure PBI membrane, PE/PBI composite membrane exhibits higher VRFB energy efficiency (88.62%–80.11%) in a current density range of 100–200 mA cm−2. And within 150 cycles at 180 mA cm−2, the composite membrane maintains stable charge-discharge performance with slower capacity decay rate, indicating a promising membrane candidate for VRFBs.
Integrative analysis of blood metabolomics and PET brain neuroimaging data for Parkinson's disease (2019)

Integrating blood metabolomics data combined with PET data considerably enhances the diagnostic discrimination power. Metabolomic signatures also indicate interesting disease-inherent changes in cellular processes, including oxidative stress response and inflammation.
Morphine Inhibits the Pituitary-Adrenal Response to Ovine Corticotropin-Releasing Hormone in Normal Subjects* (1985)

To determine the locus of opiate modulation of ACTH secretion, 11 normal subjects were given ovine corticotropin-releasing hormone (CRH) 30 min after receiving either placebo or morphine sulfate. Plasma ACTH, cortisol, arginine vasopressin (AVP), epinephrine, norepinephrine, and CRH were measured 30 min before and up to 150 min after CRH administration. Morphine blunted the ACTH response for the first 60 min and cortisol response for the first 90 min after CRH administration. Morphine did not lower arginine vasopressin or catecholamine levels. To determine whether morphine's effect on ACTH and cortisol was due to a direct action on the corticotroph cell, dispersed rat pituitary cells were perifused with medium containing 1 microgram/ml morphine sulfate or medium alone. Morphine had no effect on the ACTH response of these cells to 10 nM CRH pulses. Similarly, morphine had no effect on ACTH production by dispersed rat pituitary cells in monolayer culture in response to 90- and 180-min incubations with 5 nM CRH. We conclude that morphine blunts the early response of the pituitary gland to CRH in vivo. Based on the lack of a direct effect of morphine on rat pituitary cells in vitro, we postulate that morphine given in vivo may modulate the pituitary ACTH response to CRH through other suprapituitary factors.
Cloud Computing Adoption at Higher Education Institutions in Developing Countries: A Qualitative Investigation of Main Enablers and Barriers (2017)

The extensive use of cloud computing is changing the way of dealing with information and communication technology. Cloud computing has created a new concept to deal with software services and hardware infrastructure. Some benefits are immediate; for instance, to allow students to share their information easily and to discover new experiences of the education system. However, despite the comprehensive literature, there is still limited studies related to the applied factors that influence the adoption of cloud technology at higher education institutions. Such limitations in the literature arise clearly in developing countries. This paper attempts to enhance the understanding of related factors affecting the adoption of cloud computing in the educational context of developing nations. A fieldwork study for collecting data has been conducted at higher education institutions in the kingdom of Jordan. This study applied an interpretive paradigm with a qualitative research method to investigate the main enablers of and barriers to cloud computing adoption at the universities in developing countries in general and in the case of Jordan in particular.