gebhart/patrepeval huggingface dataset:
Mammalian glucokinase and its gene (1993)

Research Article| July 01 1993 Mammalian glucokinase and its gene P B Iynedjian P B Iynedjian 1Division of Clinical Biochemistry, University of Geneva School of Medicine, 1 rue Michel Servet, CH-1211 Geneva 4, Switzerland Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1993) 293 (1): 1–13. https://doi.org/10.1042/bj2930001 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation P B Iynedjian; Mammalian glucokinase and its gene. Biochem J 1 July 1993; 293 (1): 1–13. doi: https://doi.org/10.1042/bj2930001 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1993 The Biochemical Society, London1993 Article PDF first page preview Close Modal You do not currently have access to this content.
MiR-205 Is Progressively Down-Regulated in Lymph Node Metastasis but Fails as a Prognostic Biomarker in High-Risk Prostate Cancer (2013)

The treatment of high-risk prostate cancer (HRPCa) is a tremendous challenge for uro-oncologists. The identification of predictive moleculobiological markers allowing risk assessment of lymph node metastasis and systemic progression is essential in establishing effective treatment. In the current study, we investigate the prognostic potential of miR-205 in HRPCa study and validation cohorts, setting defined clinical endpoints for both. We demonstrate miR-205 to be significantly down-regulated in over 70% of the HRPCa samples analysed and that reconstitution of miR-205 causes inhibition of proliferation and invasiveness in prostate cancer (PCa) cell lines. Additionally, miR-205 is increasingly down-regulated in lymph node metastases compared to the primary tumour indicating that miR-205 plays a role in migration of PCa cells from the original location into extraprostatic tissue. Nevertheless, down-regulation of miR-205 in primary PCa was not correlated to the synchronous presence of metastasis and failed to predict the outcome for HRPCa patients. Moreover, we found a tendency for miR-205 up-regulation to correlate with an adverse outcome of PCa patients suggesting a pivotal role of miR-205 in tumourigenesis. Overall, we showed that miR-205 is involved in the development and metastasis of PCa, but failed to work as a useful clinical biomarker in HRPCa. These findings might have implications for the use of miR-205 as a prognostic or therapeutic target in HRPCa.
Contrast ratio dynamic range: A new beamformer performance metric (2017)

The beamformer plays a crucial role in increasing the quality of images acquired with an ultrasound imaging system. Numerous beamforming methods have been introduced, evaluated and reported with substantial improvement in resolution and contrast, compared with conventional delay-and-sum (DAS) beamforming. Better beamforming should not only provide high quality images, but the image should also be accurate and reliable. To address these problems, we propose a new beamformer performance metric that predicts how well a beamformer will preserve intrinsic contrast. We call the proposed metric contrast ratio dynamic range. We quantified it as the longest continuous duration along the true contrast line. We then applied it to several beamformers. In no added clutter case, the dynamic range using DAS, DAS+CF, MV, SLSC and ADMIRE with low and high degrees of freedom (df), are 56.3 dB, 21.0 dB, 55.5 dB, 17.1 dB, 28.5 dB and 58.1 dB, respectively.
Association of oral health with risk of incident micro and macrovascular complications: A prospective cohort study of 24,862 people with diabetes (2023)

Self-reported oral health measures may be a marker of elevated risk of complications in people with diabetes.
Identification of an Androgen Response Element in Intron 8 of the Sterol Regulatory Element-binding Protein Cleavage-activating Protein Gene Allowing Direct Regulation by the Androgen Receptor (2004)

Sterol regulatory element-binding proteins (SREBPs) are transcription regulators that play a pivotal role in intracellular lipid homeostasis. They are synthesized as inactive precursor proteins in the endoplasmic reticulum, where they are retained by SREBP cleavage-activating protein (SCAP), a sterol sensing protein that in turn is linked to a retention protein complex. Low intracellular sterol concentrations weaken the interaction of SCAP with its retention proteins and allow translocation of the SREBP.SCAP complex to the Golgi compartment where SREBP is proteolytically cleaved and activated. Previous studies on the mechanisms by which androgens provoke a coordinated activation of lipogenic pathways in prostate cancer cells have suggested an alternative pathway of activation in which androgens increase the expression of SCAP and favor translocation of the SREBP.SCAP complex to the Golgi apparatus by disturbing the balance between SCAP and its retention proteins. Here we show that the SCAP gene contains an androgen-responsive region located in intron 8. This region interacts directly with the androgen receptor and confers androgen responsiveness to promoter-reporter constructs transfected in LNCaP cells. It contains a noncanonical androgen response element GGAAGAaaaTGTACC that interacts not only with the androgen receptor but also with the glucocorticoid receptor and that also confers glucocorticoid responsiveness. The identification of a steroid response element in intron 8 of the SCAP gene further supports the contention that SCAP is a direct target for steroid hormone action.
A model of the interaction of T lymphocytes with electromagnetic waves. (2014)

This paper discusses a model of T lymphocytes and of their life-cycle which explains numerous experimental results that were generally considered as mutually incoherent. The interaction between a T Cell Receptor (TCR) and a peptide-Major Histocompatibility Complex (pMHC) is assumed to be based on a recognition mechanism, associated with negative thymus selection, and a non-recognition mechanism, associated with positive thymus selection. Each of these mechanisms is modelled using three quantum wells and transitions between these quantum wells naturally stimulated by the thermal electric field. Stimulation of the transitions with artificial electromagnetic waves in addition to the thermal background determines experimentally observed effects. The model is confronted to experimental results concerning in vivo or in vitro inhibition or stimulation of T lymphocytes by various types of waves, increased or decreased cancer growth rates, auto-immune effects and multi-generational studies on rats, and therapies including hyperthermia, the Lakhovsky multiple waves oscillator and the Priore systems.
Container System for Enabling Commercial Production of Cryopreserved Cell Therapy Products (2010)

Pharmaceutical-grade plastic CZ vials, which are commercially used to package pharmaceutical products, are suitable for low-temperature storage and transport of mesenchymal stem cells, and are a scalable container system for commercial manufacturing and fill-finish operation of cell therapy products.
Noise Source Identification and Noise Directivity Analysis of Bladeless Fans by Combined CFD and CAA Method (2020)

Abstract The bladeless fan is a new concept of fan that does not have visible impellers. It features low noise level, uniform airflow, and improved safety. It has been widely applied in household appliances. Since the customers are particularly sensitive to the noise generated by the fan, the aeroacoustics performance of the fan needs to be accurately characterized in the design stage. In this study, computational fluid dynamic (CFD) and computational aeroacoustics (CAA) are applied to investigate the aeroacoustics performance and identify the major noise source of the bladeless fan. A prototype of the bladeless fan, including a wind channel, a base cavity, a rotor and a stator inside the base, is set in a computational domain of 4m × 2m × 2m and the airflow through the fan is simulated. The hybrid mesh is generated, the unstructured mesh in the near field, and the structured at the far field. To compute the flow field, steady RANS simulation (standard k–ε turbulence model) and Large Eddy simulation (Smagorinsky-Lilly model) are carried out. Ffowcs Williams and Hawkings (FW-H) analogy is used to predict the acoustic field. Experiments, including air velocity measurement and sound pressure measurement, are conducted to validate simulation results. Sound pressure level results at the near-field receiver illustrate that the blade passage frequency can be captured by combined CFD and CAA method. Noise source analysis shows that the combination of the rotor and stator contributes most to the noise produced by the bladeless fan. The wind channel is the secondary source. Sound pressure level contours at different distances and different heights are generated to investigate the directivity pattern of the noise generated by the bladeless fan. At the near field, the produced noise at the front and the back of the bladeless fan are louder than those at left and right; at the far field, the noise at the front is much larger than the other three sides. In addition, at the near field, with the increase of the height, two separated hotspots appear over 2,500Hz and the sound pressure level at these two hotspots increases; at the far field, the noise distribution at different heights is similar and the peak near 3,000Hz can be estimated. A possible reason to cause this peak is vortex shedding at the trailing edge of the rotor’s blades. The aeroacoustics analysis is helpful to develop strategies to reduce noise and guide the improved design of the bladeless fan.
Time-triggered high-confidence embedded systems: modeling, simulation, analysis and back (2011)

The process and tools for designing time-triggered high-confidence embedded systems should encompass as many useful aspects of a system's behavior as possible in order to create a more comprehensive understanding of the system. The research presented in this thesis is not intended to capture every detail of these systems, but instead aims for a subset of behaviors that are of use to system designers. This research, in conjunction with other research being conducted on ESMoL, provides a useful theoretical basis and a set of practical modeling tools for high-confidence time-triggered embedded systems.
Joint Video and Text Parsing for Understanding Events and Answering Queries (2014)

This article proposes a multimedia analysis framework to process video and text jointly for understanding events and answering user queries. The framework produces a parse graph that represents the compositional structures of spatial information (objects and scenes), temporal information (actions and events), and causal information (causalities between events and fluents) in the video and text. The knowledge representation of the framework is based on a spatial-temporal-causal AND-OR graph (S/T/C-AOG), which jointly models possible hierarchical compositions of objects, scenes, and events as well as their interactions and mutual contexts, and specifies the prior probabilistic distribution of the parse graphs. The authors present a probabilistic generative model for joint parsing that captures the relations between the input video/text, their corresponding parse graphs, and the joint parse graph. Based on the probabilistic model, the authors propose a joint parsing system consisting of three modules: video parsing, text parsing, and joint inference. Video parsing and text parsing produce two parse graphs from the input video and text, respectively. The joint inference module produces a joint parse graph by performing matching, deduction, and revision on the video and text parse graphs. The proposed framework has the following objectives: to provide deep semantic parsing of video and text that goes beyond the traditional bag-of-words approaches; to perform parsing and reasoning across the spatial, temporal, and causal dimensions based on the joint S/T/C-AOG representation; and to show that deep joint parsing facilitates subsequent applications such as generating narrative text descriptions and answering queries in the forms of who, what, when, where, and why. The authors empirically evaluated the system based on comparison against ground-truth as well as accuracy of query answering and obtained satisfactory results.
Management of fusarium wilt of chickpea using brassicas as biofumigants (2017)

Phasing out of methyl bromide has resulted in the need for alternative strategies for the management of soilborne pests and diseases. Among several bioactive molecules, glucosinolates from Brassicaceae and their enzymatic degradation products, especially isothiocyanates formed by myrosinase enzyme, stand out as a promising alternative biofumigants. The effectiveness of biocidal compounds released from brassica species {Sinapis alba L. (syn. Brassica alba L.), B. nigra L., B. napus L., B. rapa L., B. juncea L. and B. carinata} tissue degradation on Fusarium oxysporum f. sp. ciceris (Padwick) Matuo and K.Sato (F.o.c; causal agent of chickpea wilt) growth in vitro, propagule attrition in soil and incidence of chickpea wilt in the field was carried out. Brassica alba L. was found to be the most toxic as it resulted in maximum percent mycelial growth inhibition of the pathogen followed by B. nigra L. and B. juncea L. tissues. Under glasshouse conditions, B. nigra was found most effective in reducing the colony forming units of the pathogen followed by B. alba L. and B. juncea L.. Lowest wilt severity under field conditions was observed in B. juncea L. tissues while greatest grain yield was produced in B. alba L. amended plots. The present study showed that the soil amendment with brassicas might be a feasible non-chemical approach to manage fusarium wilt of chickpea.
Evaluating the non-isothermal crystallization behavior of organic molecules from the undercooled melt state using rapid heat/cool calorimetry (2012)

Understanding the inherent crystallization tendency of organic molecules from the undercooled melt state is an important criterion to assess the glass-forming ability of a compound, and hence the ability to produce and maintain amorphous materials, an important area for drug delivery of poorly water-soluble therapeutics. The main objective of this study was to determine the critical cooling (Rcrit,cool) and/or critical heating (Rcrit,heat) rates to inhibit crystallization for a group of compounds with diverse chemistries and different crystallization tendencies. Compounds were cooled at various cooling/heating rates using a prototype rapid heat/cool (RHC) calorimeter, and crystallization (or lack thereof) from the undercooled melt was recorded. Results showed some compounds have very high crystallization tendencies [class (I–A)] from the undercooled melt state, with Rcrit,cool rates above 1000 °C min−1, while some compounds with low crystallization tendencies upon cooling [class (II) compounds] exhibited very high crystallization tendencies (Rcrit,heat > 1000 °C min−1) upon subsequent reheating from the amorphous state. The pronounced asymmetry observed between Rcrit,cool and Rcrit,heat rates for class (II) compounds was greatly reduced by rapidly quenching the compound from the melt state, preventing the formation of quenched-in nuclei during cooling and initiating crystallization upon reheating. The ability to apply controlled rapid heating and cooling rates to organic compounds thus provides useful insight into the crystallization tendency of organic compounds.
Antiproliferative effects of halichondrin B analog eribulin mesylate (E7389) against paclitaxel-resistant human cancer cells in vitro (2007)

C58 Eribulin (E7389, ER-086526, NSC-707389), a structurally-simplified synthetic analog of the biologically active macrocyclic portion of the marine natural product halichondrin B, is currently undergoing Phase 3 clinical trials for metastatic breast cancer. Preclinical studies have shown that eribulin exerts potent anticancer activities in vitro and in vivo by inhibiting microtubule dynamics via a mechanism distinct from other tubulin-targeted agents. Eribulin inhibits proliferation of a wide spectrum of human cancer cell types at sub-nanomolar concentrations and shows impressive anticancer activity against a broad range of human cancer xenograft models in mice, including complete tumor regressions. To further evaluate eribulin as an anticancer agent, its activity against drug-resistant human cancer cells was studied. Cancer cells can acquire resistance to chemotherapy agents by several different mechanisms. One mechanism with particular relevance to some tubulin-targeted drugs involves mutations in β-tubulin that result in decreased responsiveness to tubulin-targeted agents such as the taxanes. In this study, antiproliferative effects of eribulin were evaluated in an in vitro cell-based model of resistance based on mutated β-tubulin. Three ovarian cancer cell lines, A2780/1A9, 1A9PTX10 and 1A9PTX22, were obtained from the laboratory of Dr. Tito Fojo, US National Cancer Institute. The two paclitaxel resistant sublines of A2780/1A9, 1A9PTX10 and 1A9PTX22, were originally isolated as individual clones in a single step selection following exposure of A2780/1A9 cells to paclitaxel. Both sublines harbor different mutations in the M40 β-tubulin isotype and have been shown to have significant resistance to paclitaxel. To verify that paclitaxel resistance of 1A9PTX10 and 1A9PTX22 cells is not based on expression of the multidrug resistance efflux pump P-glycoprotein (PgP), cells were stained with fluorescently-labeled anti-PgP antibody and analyzed by flow cytometry. Neither of the 2 resistant lines, 1A9PTX10 or 1A9PTX22, nor parental A2780/1A9 cells, expressed detectable PgP, indicating that paclitaxel resistance seen in 1A9PTX10 and 1A9PTX22 cells is not PgP-mediated. Antiproliferative effects of eribulin, paclitaxel and vinblastine were then measured in A2780/1A9, 1A9PTX10 and 1A9PTX22 cells. Consistent with literature reports, 1A9PTX10 and 1A9PTX22 cells showed 15- to 19-fold resistance to paclitaxel. In contrast, 1A9PTX10 and 1A9PTX22 cells showed almost identical sensitivities to eribulin and vinblastine compared with parental A2780/1A9 cells (IC 50 ratios ~1.3). These results indicate that eribulin and vinblastine retain essentially full in vitro potency in cells harboring β-tubulin mutations that lead to substantial resistance to paclitaxel.
Anti-GRP78 autoantibodies induce endothelial cell activation and accelerate the development of atherosclerotic lesions (2018)

The 78-kDa glucose-regulated protein (GRP78) is an ER molecular chaperone that aids in protein folding and secretion. However, pathological conditions that cause ER stress can promote the relocalization of GRP78 to the cell surface (csGRP78), where it acts as a signaling receptor to promote cancer progression. csGRP78 also possesses antigenic properties, leading to the production of anti-GRP78 autoantibodies, which contribute to tumor growth. In contrast, the presence and role of anti-GRP78 autoantibodies in atherosclerosis is unknown. Here, we show that atherosclerotic-prone ApoE-/- mice develop circulating anti-GRP78 autoantibodies that bind to csGRP78 on lesion-resident endothelial cells. Moreover, GRP78-immunized ApoE-/- mice exhibit a marked increase in circulating anti-GRP78 autoantibody titers that correlated with accelerated lesion growth. Mechanistically, engagement of anti-GRP78 autoantibodies with csGRP78 on human endothelial cells activated NF-κB, thereby inducing the expression of ICAM-1 and VCAM-1, a process blocked by NF-κB inhibitors. Disrupting the autoantibody/csGRP78 complex with enoxaparin, a low-molecular-weight heparin, reduced the expression of adhesion molecules and attenuated lesion growth. In conclusion, anti-GRP78 autoantibodies play a crucial role in atherosclerosis development, and disruption of the interaction between anti-GRP78 autoantibodies and csGRP78 represents a therapeutic strategy.
Synthesis of Water-Soluble, Nonimmunogenic Polyamide Cross-Linking Agents (1998)

Novel polyamides were developed that can be used as cross-linking agents for proteins such as hemoglobin. Water-soluble, nonimmunogenic polyamides containing oxygen and sulfur atoms in the backbone were prepared by the polycondensation of the diacids bis(carboxymethyloxyacetyl)-1,4-diaminobutane (1a) or 3, 3'-thiodipropionic acid (1b) with diethylene glycol bis(3-aminopropyl) ether (2). The resulting alpha,omega-diacids were converted to the corresponding activated esters using any of a variety of carboxylic acid activating reagents including the novel reagent diphenyl(1-methylimidazol-2-thiyl)phosphonate (9). The resulting polyamides could be activated with a broad spectrum of groups that allow for the cross-linking and surface modification of proteins.
The effect of endpoint congruency on bimanual transport and rotation tasks (2015)

The completion of many goal oriented skills requires the tight coordination of the right and left hands to achieve the task objective. Although the coordination of wrist transport and orientation of the hand before object contact has been studied in detail for discrete bimanual tasks, as yet, very few studies have examined bimanual coordination when the target is already in hand. It has been shown that congruency of the goal facilitates the production of discrete bimanual responses. The purpose of this study was to investigate the role of goal congruency on precision bimanual transport and rotate tasks. In the current investigation, participants transported two cubic objects while rotating them laterally to place them into tight-fitting targets. The magnitude of the rotation could be the same for both hands (i.e., both 45 or 90(∘)) or different (i.e., one 45 and 90(∘)) and the endpoint orientations (i.e., goal) could either be congruent or incongruent. Results indicated that when the endpoint orientation was congruent for the two hands, movement times were similar regardless of hand (left or right), rotation magnitude (45, 90(∘)) and whether the rotation magnitude for the two hands was the same or different. These results suggest that congruency of the endpoint goal facilitates the temporal synchronization of the transport component for two limbs. In contrast, a different pattern of results was obtained when considering the rotation component. Specifically, regardless of whether the hands were rotating the same magnitude or ending in congruent endpoint positions, the coordination of the rotation component between the hands was asynchronous. We hypothesize that the greater requirement to shift visual fixation from one hand/target to the other to ascertain the separate goal orientations may explain these differences. These results provide further evidence that multiple constraints act to influence the performance of skilled bimanual tasks.