gebhart/patrepeval huggingface dataset:
Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection (2017)

Objectives Feline leukaemia virus (FeLV), a gamma retrovirus, causes diseases of the feline haematopoietic system that are invariably fatal. Rapid and accurate testing at the point-of-need (PON) supports prevention of virus spread and management of clinical disease. This study evaluated the performance of an insulated isothermal PCR (iiPCR) that detects proviral DNA, and a reverse transcription (RT)-iiPCR that detects both viral RNA and proviral DNA, for FeLV detection at the PON. Methods Mycoplasma haemofelis, feline coronavirus, feline herpesvirus, feline calicivirus and feline immunodeficiency virus were used to test analytical specificity. In vitro transcribed RNA, artificial plasmid, FeLV strain American Type Culture Collection VR-719 and a clinical FeLV isolate were used in the analytical sensitivity assays. A retrospective study including 116 clinical plasma and serum samples that had been tested with virus isolation, real-time PCR and ELISA, and a prospective study including 150 clinical plasma and serum samples were implemented to evaluate the clinical performances of the iiPCR-based methods for FeLV detection. Results Ninety-five percent assay limit of detection was calculated to be 16 RNA and five DNA copies for the RT-iiPCR, and six DNA copies for the iiPCR. Both reactions had analytical sensitivity comparable to a reference real-time PCR (qPCR) and did not detect five non-target feline pathogens. The clinical performance of the RT-iiPCR and iiPCR had 98.82% agreement (kappa[κ] = 0.97) and 100% agreement (κ = 1.0), respectively, with the qPCR (n = 85). The agreement between an automatic nucleic extraction/RT-iiPCR system and virus isolation to detect FeLV in plasma or serum was 95.69% (κ = 0.95) and 98.67% (κ = 0.85) in a retrospective (n = 116) and a prospective (n = 150) study, respectively. Conclusions and relevance These results suggested that both RT-iiPCR and iiPCR assays can serve as reliable tools for PON FeLV detection.
Iron Status in Mice Carrying a Targeted Disruption of Lactoferrin (2002)

Lactoferrin is a member of the transferrin family of iron-binding glycoproteins present in milk, mucosal secretions, and the secondary granules of neutrophils. While several physiological functions have been proposed for lactoferrin, including the regulation of intestinal iron uptake, the exact function of this protein in vivo remains to be established. To directly assess the physiological functions of lactoferrin, we have generated lactoferrin knockout (LFKO(-/-)) mice by homologous gene targeting. LFKO(-/-) mice are viable and fertile, develop normally, and display no overt abnormalities. A comparison of the iron status of suckling offspring from LFKO(-/-) intercrosses and from wild-type (WT) intercrosses showed that lactoferrin is not essential for iron delivery during the postnatal period. Further, analysis of adult mice on a basal or a high-iron diet revealed no differences in transferrin saturation or tissue iron stores between WT and LFKO(-/-) mice on either diet, although the serum iron levels were slightly elevated in LFKO-/- mice on the basal diet. Consistent with the relatively normal iron status, in situ hybridization analysis demonstrated that lactoferrin is not expressed in the postnatal or adult intestine. Collectively, these results support the conclusion that lactoferrin does not play a major role in the regulation of iron homeostasis.
Rapid, on-site diagnosis of chlamydial urethritis in men by detection of antigens in urethral swabs and urine (1991)

First-void urine (FVU) sediments of 240 men were tested for Chlamydia trachomatis antigens by two enzyme immunoassays, TestPack Chlamydia (15 min) and Chlamydiazyme (3.5 h), and the results were compared with urethral swab culture results. The sensitivity and specificity on FVU sediment for TestPack Chlamydia were 76.2% (32 of 42 specimens) and 95.5% (189 of 198 specimens) versus 81.0% (34 of 42 specimens) and 96.5% (191 of 198 specimens) for Chlamydiazyme, respectively. Rapid, on-site detection of chlamydial antigen in male FVU would shorten the infectious period by hastening diagnosis and treatment.
Superdense codes transmitted over optical fiber links decoded deterministically using time-polarization hyperentanglement (2017)

We report the first demonstration of superdense coding over optical fiber links, taking advantage of a complete Bell-state measurement enabled by time-polarization hyperentanglement, linear optics, and common single-photon detectors. We demonstrate the highest single-qubit channel capacity to date encoding on a single-degree of freedom utilizing linear optics, 1.665±0.018, and we provide a full experimental implementation of a hybrid, quantum-classical communication protocol for image transfer.
INTRA-FRAME PREDICTION FOR HIGH-PASS FRAMES IN MOTION COMPENSATED WAVELET VIDEO CODING (2006)

In the existing inter-frame wavelet video coding techniques, the poorly matched pixels from adjacent frames are temporally filtered into the high-pass temporal frame in the same way as the good matches using motioncompensated prediction (MCP). This tends to result in high-energy blocks being introduced into the high-pass frame, which leads to less efficient spatial coding of the affected frames. We propose to use prediction from spatial neighbouring pixels for high-pass filtering of the poorly matched blocks, which results in a significantly improved performance at low bitrates with no loss at high bitrates.
Communication between the extracellular environment, cytoplasmic signalling cascades and the nuclear cell‐cycle machinery (1997)

In the past decade, we have gained considerable insight into the identities of various cytoplasmic signal transduction cascades and the manner in which they operate in response to changes in the extracellular environment. Moreover, we have begun to understand what the key players are in cell-cycle regulation and how they, in turn, function to promote cell division. A long-standing question, however, has been how communication between signalling routes and the cell-cycle machinery occurs. This review highlights some recent observations that provide possible links between signal transduction and the cell-cycle machinery.
Characterization of dermal fibroblast-derived iPSCs from a patient with high grade steatosis (2016)

Primary fibroblasts from a high grade steatosis patient were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. IPSCs were characterized by immunocytochemistry, embryoid body-formation, DNA-fingerprint, karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.9287. Resource table.
Plasma-Enhanced Chemical Vapor Deposited SiNx and SiO2 Passivation Effects on ZnO Heterojunction Light Emitting Diodes (2008)

ZnO heterojunction light emitting diodes (LEDs) were passivated by plasma-enhanced chemical vapor-deposited SiO2 and SiNx. Post-dielectric deposition annealing was critical in obtaining good LED electrical and optical characteristics. No diode characteristics or light emission were observed unless the structures were annealed at 350 {degree sign}C after fabrication. Annealed diodes showed band-edge electroluminescence (385 nm) and a broad defect band with a peak at 930 nm at room temperature. The SiO2 and SiNx had very different passivation effects in terms of the electrical and electro-luminescence characteristics of the LEDs. After annealing, the SiO2 passivated ZnO LEDs showed diode IV characteristics and emitted light. However, the annealed SiNx-passivated ZnO LEDs showed leaky diode characteristics and no light emission. We attribute these differences to the role of hydrogen on the LEDs.
Six-month outcomes from a randomized controlled trial of minimally invasive SI joint fusion with triangular titanium implants vs conservative management (2016)

In patients with chronic SIJ pain, minimally invasive SIJF using triangular titanium implants was safe and more effective than CM in relieving pain, reducing disability, improving patient function and quality of life.
Elevated Plasma Surfactant Protein-B Predicts Development of Acute Respiratory Distress Syndrome in Patients with Acute Respiratory Failure (2001)

Surfactant protein-B is a lung specific protein secreted into the air spaces by pulmonary epithelial type II cells that leaks into the bloodstream in increased amounts in patients with ARDS. To test whether elevated plasma levels of surfactant protein-B would predict the development of ARDS in patients with acute hypoxemic respiratory failure, plasma and lung injury scores were collected at study entry and daily thereafter for 3 d from 54 patients admitted to our intensive care unit. ARDS was defined as a new bilateral infiltrate on chest radiograph and a lung injury score > or = 2.5. Twenty patients developed ARDS, of whom seven died. Although the initial lung injury score was not predictive of ARDS, the initial plasma surfactant protein-B was predictive (area under the curve = 0.77 [0.63 to 0.90], nonparametric receiver-operating characteristic analysis). In this cohort, plasma surfactant protein-B was particularly predictive of ARDS when applied to patients suffering a direct lung insult (area under the curve = 0.87 [0.72 to 1.02]), with a sensitivity of 85% (95% CI: 55 to 98%) and specificity of 78% (40 to 97%) at a cutoff of 4,994 ng/ml.
Structure of trans-diiodobis(methyl phenyl telluride)platinum(II) (1992)

[PtI 2 (C 7 H 8 Te) 2 ] cristallise dans I2/a avec a=23,807, b=9,548, c=24,192A, β=136,48°, Z=8; affinement jusqu'a R=0,041. La structure est composee de molecules planes carrees discretes de type trans. Longueurs des liaisons Pt-I=2,604, 2,607A, Pt-Te=2,578, 2,586A
Letter position coding in printed word perception: Effects of repeated and transposed letters (2004)

We report four experiments investigating the effects of repeated and transposed letters in orthographic processing. Orthographically related primes were formed by removing one letter from the target word, by transposing two adjacent letters, or by replacing two adjacent letters with different letters. Robust masked priming in a lexical decision task was found for primes formed by removing a single letter (e.g., mircle-MIRACLE), and this was not influenced by whether or not the prime contained a letter repetition (e.g., balace vs. balnce as a prime for BALANCE ). Target words containing a repeated letter tended to be harder to respond to than words without a letter repetition, but the nonwords formed by removing a repeated letter (e.g., BALNCE) were no harder to reject than nonwords formed by removing a non-repeated letter (e.g., MIRCLE, BALACE). Significant transposition priming effects were found for 7-letter words (e.g., sevrice-SERVICE), and these priming effects did not vary as a function of the position of the transposition (initial, final, or inner letter pair). Priming effects disappeared when primes were formed by replacing the two transposed letters with different letters (e.g., sedlice-SERVICE), and five-letter words only showed priming effects with inner letter transpositions (e.g., ponit-POINT). We present a revised “open-bigram” scheme for letter position coding that accounts for these data.
Systematic Review On Pleurectomy/Decortication In The Multimodality Treatment of Malignant Pleural Mesothelioma (2014)

SUMMARY Malignant pleural mesothelioma (MPM) is an aggressive tumor of pleural membranes with a dismal prognosis. Following diagnosis survival is usually less than 12 months. The treatment is therefore very often palliative. In selected cases with early stage disease surgical resection is possible. Several studies have now shown that combination treatment with chemotherapy (CHT), radiotherapy (RT) and radical surgery lead to an increased overall survival (OS). This review analyzes the long-term results of the two different types of radical surgery included in the multimodality treatment: extrapleural pneumonectomy (EPP) and pleurectomy/decortication (P/D). Recent studies have shown P/D to have less morbidity with comparable or better OS compared with EPP while enabling more patients to complete multimodality treatment.
Refinement-based design of a group-centric secure information sharing model (2012)

This paper presents a formal, state machine-based specification (stateful specification) of a group-centric secure information sharing (g-SIS) model. The stateful specification given here is a refinement of a prior specification that is given in first-order linear temporal logic (FOTL). Such FOTL specification defines authorization based solely on group operations, but gives little guidance regarding implementation. The current specification is the result of a second step in a multi-step design process that separates concerns and provides multiple opportunities to detect unintended policy characteristics. We show that our stateful specification is consistent with the prior FOTL specification by using a combination of model-checking and manual techniques.
Medhere: A Smartwatch-based Medication Adherence Monitoring System using Machine Learning and Distributed Computing (2018)

Poor medication adherence threatens an individual's health and is responsible for substantial medical costs in the United States annually. In order to improve medication adherence rates and provide timely reminders, we developed a smartwatch application that collects data from embedded inertial sensors, which include an accelerometer and gyroscope, to monitor a series of actions happening during an individual's medication intake. After the collected data was delivered to a server, Apache Spark was used to distribute the data and apply machine learning algorithms in order to predict several discrete actions including medication intake. By utilizing these tools, we were able to preprocess high frequency sensor data and apply a random forest algorithm, yielding high frequency and recall of the aforementioned actions.
Hypoxia and vascular endothelial growth factor expression in human squamous cell carcinomas using pimonidazole as a hypoxia marker. (1998)

Hypoxia in human tumors is associated with poor prognosis, but the molecular mechanisms underlying this association are poorly understood. One possibility is that hypoxia is linked to malignant progression through vascular endothelial growth factor (VEGF) induction and the associated angiogenesis and metastasis. The present clinical study measures hypoxia and VEGF expression on a cell-by-cell basis in human squamous cell carcinomas to test the hypothesis that hypoxia and VEGF protein expression are coupled in human tumors. Eighteen patients with invasive squamous cell carcinoma of the uterine cervix and head and neck have been investigated by a quantitative image analysis of immunostained sections from their tumors. The hypoxia marker pimonidazole was used to measure tumor hypoxia, and a commercially available antibody was used to measure VEGF protein expression. A quantitative immunohistochemical comparison of hypoxia and VEGF protein expression revealed no correlation between the two factors.
Emerging Trends in Search Interfaces (2009)

This chapter describes several areas that are gaining in importance and are likely to play a significant role in search interfaces in the future. These predictions come primarily from extrapolating out from emerging trends. The areas that seem most likely to be important, and so demand changes in search interface technology, are: mobile search, multimedia search, social search, and a hybrid of natural language and command-based queries in search. Each is discussed in detail below.
Le programme de soin : de la dépendance à l'autonomie ? (2014)

Cet écrit interroge la dynamique institutionnelle des soins psychiatriques sous contrainte, et notamment leur déploiement à travers le programme de soin ambulatoire, dispositif soutenu par la loi de juillet 2011. En travaillant les notions d’autonomie et de dépendance dans leur lien avec la pathologie mentale et avec l’idée du consentement des patients, nous tentons de décrire les pratiques actuelles de la psychiatrie sectorielle et de dégager le positionnement spécifique que peut soutenir le psychologue dans ses interventions cliniques et institutionnelles.
Submovements grow larger, fewer, and more blended during stroke recovery. (2003)

Submovements are hypothesized building blocks of human movement, discrete ballistic movements of which more complex movements are composed. Using a novel algorithm, submovements were extracted from the point-to-point movements of 41 persons recovering from stroke. Analysis of the extracted submovements showed that, over the course of therapy, patients' submovements tended to increase in peak speed and duration. The number of submovements employed to produce a given movement decreased. The time between the peaks of adjacent submovements decreased for inpatients (those less than 1 month post-stroke), but not for outpatients (those greater than 12 months post-stroke) as a group. Submovements became more overlapped for all patients, but more markedly for inpatients. The strength and consistency with which it quantified patients' recovery indicates that analysis of submovement overlap might be a useful tool for measuring learning or other changes in motor behavior in future human movement studies.
Beneficial Storage Effects of Epigallocatechin-3-O-Gallate on the Articular Cartilage of Rabbit Osteochondral Allografts (2009)

A fresh osteochondral allograft is one of the most effective treatments for cartilage defects of the knee. Despite the clinical success, fresh osteochondral allografts have great limitations in relation to the short storage time that cartilage tissues can be well-preserved. Fresh osteochondral grafts are generally stored in culture medium at 4 degrees C. While the viability of articular cartilage stored in culture medium is significantly diminished within 1 week, appropriate serology testing to minimize the chances for the disease transmission requires a minimum of 2 weeks. (-)-Epigallocatechin-3-O-gallate (EGCG) has differential effects on the proliferation of cancer and normal cells, thus a cytotoxic effect on various cancer cells, but a cytopreservative effect on normal cells. Therefore, a storage solution containing EGCG might extend the storage duration of articular cartilages. Rabbit osteochondral allografts were performed with osteochondral grafts stored at 4 degrees C in culture medium containing EGCG for 2 weeks and then the clinical effects were examined with macroscopic and histological assessment after 4 weeks. The cartilaginous structure of an osteochondral graft stored with EGCG was well-preserved with high cell viability and glycosaminoglycan (GAG) content of the extracellular matrix (ECM). After an osteochondral allograft, the implanted osteochondral grafts stored with EGCG also provided a significantly better retention of the articular cartilage with viability and metabolic activity. These data suggest that EGCG can be an effective storage agent that allows long-term preservation of articular cartilage under cold storage conditions.
Platelet reactivity with liposome-encapsulated hemoglobin in the rat. (1997)

Infusion of liposome-encapsulated hemoglobin (LEH) induces a transient thrombocytopenia in rats (Rabinovici R, Rudolph AS, Ligler FS, Smith EF, III, Feuerstein G [1992] Biological responses to exchange transfusion with liposome-encapsulated hemoglobin. Circ Shock 37:124). A specific mechanism such as a localization of platelets during this transient LEH-induced thrombocytopenia has not been reported previously in the literature. In this study, platelets were isolated and labeled with indium-111 (111In), then retransfused into the same animal. Fifteen minutes after the 111In platelets were retransfused and allowed to equilibrate with the blood pool, a 10% top load volume of either LEH (1.8 mL, 262 mg phospholipid/kg body weight, 92 mg hemoglobin (Hb)/kg body weight), liposome vehicle (LV) (1.8 mL, 262 mg phospholipid/kg body weight), or free bovine Hb (1.8 mL, 92 mg Hb/kg body weight) (n=6 per group) was infused. Serial blood samples were drawn to determine platelet counts by radioactivity and light scattering methods. LV and Hb demonstrated no significant changes from baseline levels in circulating platelet levels, whereas LEH caused a transient 50% decrease in 111In platelet activity 2-5 minutes postinfusion, which returned to baseline levels by 15 minutes. Platelet counts based on traditional light scattering methods were not significantly different among the three treatment groups over this same time course. Localization of these 111In platelets was monitored with a gamma scintigraphic camera. After infusion of LEH, 111In platelets rapidly moved out of the circulation and sequestered in the lungs and liver with subsequent return to the circulation by 15 minutes. In contrast, no significant changes in 111In platelet activity were noted in the lungs and liver after infusion of LV and Hb. 111In platelet activity in the spleen nearly doubled 30 minutes after Hb infusion and was significantly different than spleen 111In platelet activity in the LEH and LV groups. In vitro platelet aggregation studies were also performed to determine the direct effect of LEH, LV, and Hb on platelet aggregation. The rate of thrombin-induced aggregation did not differ among control samples and samples containing LEH, LV, or Hb; none of these agents induced platelet aggregation. We conclude that LEH-induced thrombocytopenia is associated with a transient (within minutes) sequestration of platelets in the lungs and liver, with subsequent release to the circulation within 15 minutes. Tetrameric bovine Hb is associated with increased platelet accumulation in the spleen. Light scattering methods for measuring platelet levels in blood samples containing LEH are unreliable because of particulate interference.
Geometrical error calibration in reflective surface testing based on reverse Hartmann test (2017)

In the fringe-illumination deflectometry based on reverse-Hartmann-test configuration, ray tracing of the modeled testing system is performed to reconstruct the test surface error. Careful calibration of system geometry is required to achieve high testing accuracy. To realize the high-precision surface testing with reverse Hartmann test, a computer-aided geometrical error calibration method is proposed. The aberrations corresponding to various geometrical errors are studied. With the aberration weights for various geometrical errors, the computer-aided optimization of system geometry with iterative ray tracing is carried out to calibration the geometrical error, and the accuracy in the order of subnanometer is achieved.
Chemoselective Reductive Amination of Aldehydes and Ketones by Dibutylchlorotin Hydride-HMPA Complex (2000)

Reductive amination of various aldehydes and ketones has been performed effectively by pentacoordinate chloro-substituted tin hydride complex, Bu2SnClH-HMPA. The tin reagent worked particularly well for the case using weakly basic aromatic amines as starting substrates. Stoichiometric amounts of a substrate and a reducing agent were adequate for the reaction. The Sn-Cl bond in the complex plays an important role for both steps of imine formation and subsequent reduction. Highly chemoselective reduction of carbonyls could be achieved regardless of other functionalities such as halogen, carbon-carbon double bond and hydroxyl groups in the starting carbonyls and amines.