gebhart/patrepeval huggingface dataset:
Covalent and Oriented Surface Immobilization of Antibody Using Photoactivatable Antibody Fc-Binding Protein Expressed in Escherichia coli (2016)

Fc-specific antibody binding proteins (FcBPs) with the minimal domain of protein G are widely used for immobilization of well-oriented antibodies onto solid surfaces, but the noncovalently bound antibodies to FcBPs are unstable in sera containing large amounts of antibodies. Here we report novel photoactivatable FcBPs with photomethionine (pMet) expressed in E. coli, which induce Fc-specific photo-cross-linking with antibodies upon UV irradiation. Unfortunately, pMet did not support protein expression in the native E. coli system, and therefore we also developed an engineered methionyl tRNA synthetase (MRS5m). Coexpression of MRS5m proteins successfully induced photoactivatable FcBP overexpression in methionine-auxotroph E. coli cells. The photoactivatable FcBPs could be easily immobilized on beads and slides via their N-terminal cysteine residues and 6xHis tag. The antibodies photo-cross-linked onto the photoactivatable FcBP-beads were resistant from serum-antibody mediated dissociation and efficiently captured antigens in human sera. Furthermore, photo-cross-linked antibody arrays prepared using this system allowed sensitive detection of antigens in human sera by sandwich immunoassay. The photoactivatable FcBPs will be widely applicable for well-oriented antibody immobilization on various surfaces of microfluidic chips, glass slides, and nanobeads, which are required for development of sensitive immunosensors.
Complex‐Induced Proximity Effects. Temperature‐Dependent Regiochemical Diversity in Lithiation—Electrophilic Substitution Reactions of N‐BOC‐2‐Azabicyclo[2.1.1]hexane. 2,4‐ and 3,5‐Methanoprolines. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Characterization of Salmonella california and S. typhimurium Strains with Reduced Ability to Colonize the Intestinal Tract of Broiler Chicks (1993)

This study determined the ability of eight strains of Salmonella and their agarsubcultured variants to colonize the intestinal tract of broiler chicks. Nalidixic-acid (NAL)-resistant and streptomycin-resistant subcultured strains (S. california 1989/A and S. typhimurium 3366/A) that persisted in the ceca of chicks in lower numbers than their NAL-resistant parent strains (1989/O and 3366/O) were selected for additional study S. typhimurium strain 3366/A was present in the ceca of chicks in lower numbers than the parent strain 3366/O when given concomitantly with the parent strain or when the two strains were given separately to different chicks. S. california 1989/A strain was present in the ceca in lower numbers than the parent strain after concomitant oral or intracloacal inoculation. Strains 3366/O and 3366/A of S. typhimurium differed in growth rates in BHI broth and cecal mucus. The lipopolysaccharide (LPS) profile indicated that LPS components present in S. california 1989/O were missing from strain 1989/A. A mutant of 1989/O--2095/R--was also LPS- and colonization-deficient.
Using surprisal and fMRI to map the neural bases of broad and local contextual prediction during natural language comprehension (2021)

Context guides comprehenders' expectations during language processing, and informationtheoretic surprisal is commonly used as an index of cognitive processing effort. However, prior work using surprisal has considered only within-sentence context, using n-grams, neural language models, or syntactic structure as conditioning context. In this paper, we extend the surprisal approach to use broader topical context, investigating the influence of local and topical context on processing via an analysis of fMRI time courses collected during naturalistic listening. Lexical surprisal calculated from ngram and LSTM language models is used to capture effects of local context; to capture the effects of broader context a new metric based on topic models, topical surprisal, is introduced. We identify distinct patterns of neural activation for lexical surprisal and topical surprisal. These differing neuro-anatomical correlates suggest that local and broad contextual cues during sentence processing recruit different brain regions and that those regions of the language network functionally contribute to processing different dimensions of contextual information during comprehension. More generally, our approach adds to a growing literature using methods from computational linguistics to operationalize and test hypotheses about neuro-cognitive mechanisms in sentence processing.
Hormone Therapy and its Effect on the Prognosis in Breast Cancer Patients (2015)

<b>Introduction:</b> Use of hormone therapy (HT) has declined dramatically in recent years. Some studies have reported that HT use before a diagnosis of breast cancer (BC) may be a prognostic factor in postmenopausal patients. This study aimed to examine the prognostic relevance of HT use before BC diagnosis. <b>Methods:</b> Four BC cohort studies in Germany were pooled, and 4492 postmenopausal patients with HT use data were identified. Patient data and tumor characteristics were compared between users and nonusers, along with overall survival (OS), distant metastasis-free survival (DMFS), and local recurrence-free survival (LRFS). Cox proportional hazards models were stratified by study center and adjusted for age at diagnosis, tumor stage, grading, nodal status, and hormone receptors. <b>Results:</b> Women with HT use before the diagnosis of BC were more likely to have a lower tumor stage, to be estrogen receptor-negative, and to have a lower grading. With regard to prognosis there were effects seen for OS, DMFS and LRFS, specifically in the subgroup of women with a positive hormone receptor. In these subgroups, BC patients had a better prognosis with previous HT use. <b>Conclusions:</b> HT use before a diagnosis of BC is associated with a more favorable prognosis in women with a positive hormone receptor status. It may be recommended that the prognostic factor HT should be documented and analyzed as a confounder for prognosis in studies of postmenopausal hormone-responsive breast cancers.
Colored stochastic shadow maps (2011)

This paper extends the stochastic transparency algorithm that models partial coverage to also model wavelength-varying transmission. It then applies this to the problem of casting shadows between any combination of opaque, colored transmissive, and partially covered (i.e., α-matted) surfaces in a manner compatible with existing hardware shadow mapping techniques. Colored Stochastic Shadow Maps have a similar resolution and performance profile to traditional shadow maps, however they require a wider filter in colored areas to reduce hue variation.
AMPA Receptor antagonist NBQX attenuates later‐life epileptic seizures and autistic‐like social deficits following neonatal seizures (2013)

Brief NBQX administration during the 48 h postseizure in P10 Long-Evans rats suppresses transient mTOR pathway activation and attenuates spontaneous recurrent seizures, social preference deficits, and mossy fiber sprouting observed in vehicle-treated adult rats after early life seizures. These results suggest that acute AMPAR antagonist treatment during the latent period immediately following neonatal HS can modify seizure-induced activation of mTOR, reduce the frequency of later-life seizures, and protect against CA3 mossy fiber sprouting and autistic-like social deficits.
Outcome of older cancer patients infected with Covid19 at Gustave Roussy Cancer Center (2020)

Background: The SARS-CoV-2 outbreak in Paris's region significantly affected Gustave Roussy Cancer Center Previous analyses showed that mortality rate increases with age in the general population Here, we report theGustave Roussy experience on older patients (OP) with cancer during the SARS-CoV-2 outbreak Methods: Cancer pts with suspected SARS-CoV-2 infection were admitted at Gustave Roussy starting March 12th Screening indications have been adapted over the time All the COVID19 pts positively tested and managed atGustave Roussy between March 14th (1st positive case) and April 15th have been included in a REDCap database Pts and underlying oncologic and COVID19 diseases characteristics have been collected Cancer and COVID-19managements and outcomes have been assessed The primary endpoint of this analysis was the clinicaldeterioration, defined as the need for O2 supplementation of 6l/min, or death of any cause Results: Among the first 137 cancer pts diagnosed with SARS-CoV-2, 36 patients were aged 70 years (26%) Mostof them were female (61%) with a median age of 75 5 years old Most frequent underlying cancers were solidtumors (92%) including GI (19%), lung (17%), GYN (14%), and head and neck (14%) Most OP (36%) were ECOGperformance status 2 versus 24% in younger patients (YP) The diagnosis of SARS-CoV-2 infection was made byRT-PCR or thoracic CT scan alone in 97% and 3% of the cases, respectively, in OP and in 92% and 8% in YP MostOP experienced symptoms prior to testing (92%) compared to YP (80%) Symptoms differed according to age withmore cough with sputum production in OP (14% versus 5%), dyspnea (39% versus 31%), diarrhea (17% versus9%), shivers (8% versus 0%), sore throat (8% versus 4%), and no anosmia or agueusia The majority of OP werehospitalized (81%) compared to 72% of YP and treated with HCQ/AZI (15;52%) with inclusion in the ONCOVID trial(EudraCT: 2020-01250-21) compared to 25 (35%) YP They did not receive any IL-6 inhibitor Only one OP wasadmitted in the ICU (3%) Clinical deterioration occurred in 10 OP (29%) There was no impact of age on clinicalworsening (HR=1 157;95%CI 0 55-2 42;p=0 7) However, age was associated with worse overall survival (OS)(HR=2 45 95%CI 1 02-5 92 ;p=0 0463) Results will be updated at the meeting Conclusions: OP with cancer had a different disease presentation, same rate of clinical worsening, but worse OSin SARS-CoV-2 infection
Voltage-controlled nuclear polarization switching in a singleInxGa1−xAsquantum dot (2009)

Sharp thresholdlike transitions between two stable nuclear-spin polarizations are observed in optically pumped individual ${\text{In}}_{x}{\text{Ga}}_{1\ensuremath{-}x}\text{As}$ self-assembled quantum dots embedded in a Schottky diode when the bias applied to the diode is tuned. The abrupt transitions lead to the switching of the Overhauser field in the dot by up to 3 T. The bias-dependent photoluminescence measurements reveal the importance of the electron-tunneling-assisted nuclear-spin pumping. We also find evidence for the resonant LO-phonon-mediated electron cotunneling, the effect controlled by the applied bias and leading to the reduction of the nuclear-spin pumping rate.
Oral intake of Lactobacillus plantarum L‐14 extract alleviates TLR2‐ and AMPK‐mediated obesity‐associated disorders in high‐fat‐diet‐induced obese C57BL/6J mice (2021)

EPS from L-14 extract inhibits adipogenesis via TLR2 and AMPK signalling pathways, and oral intake of L-14 extract improves obesity and obesity-associated diseases in vivo. Therefore, EPS can be used to prevent and treat obesity and metabolic disorders.
Recent changes in bacterial epidemiology and the emergence of fluoroquinolone-resistant Escherichia coli among patients with haematological malignancies: results of a prospective study on 823 patients at a single institution (2008)

A shift towards gram-negative bacteria has been occurring in recent years in the bacterial epidemiology of haematological patients. Fluoroquinolone resistance is emerging as a major type of antibacterial resistance, particularly among E. coli strains. Further investigation is needed to explore the consequences of such epidemiological changes.
Monosomal Karyotype in Acute Myeloid Leukemia: A Better Indicator of Poor Prognosis Than a Complex Karyotype (2008)

MK enables (in addition to CN and CBF) the prognostic classification of two new aggregates of cytogenetically abnormal AML, the unfavorable risk MK-negative category (4-year OS, 26% +/- 2%) and the highly unfavorable risk MK-positive category (4-year OS, 4% +/- 1%).
High accuracy sensor for dynamic measurements based on holographic optical elements, semiconductor lasers and detectors (1994)

Diffractive optical elements have been of interest as far back as in 1872 where Lord Rayleigh described the fabrication of zone plates by contact copying ruled gratings onto various photorecording materials obtaining higher diffraction efficiency than that of the original. The introduction of the laser brought about a means for recording high pitched gratings as holograms. Recording materials with specific characteristics became important for rendering high diffraction efficiencies, ease of processing, good reproducibility, and high sensitivity and resolution. Combining this with low-cost lasers, detectors, and detector arrays provides new possibilities for optical sensors.
Nd–Fe–B Thick-Film Magnets on Si Substrates With Al–O Underlayers (2023)

We previously developed thick Nd–Fe–B films on Si substrates with glass underlayers using pulsed laser deposition (PLD). However, the glass layers had inhomogeneous structures, with voids due to the emission of large particles (droplets), making them unsuitable for micromagnetization. Additionally, knicks in the films’ hysteresis loop squareness were observed. In this study, we present a novel approach to fabricating Nd–Fe–B films on an Al–O film (with a target composition of Al2O3) instead of a glass film on a Si substrate. We observed a reduction in the number and sizes of large particles (droplets) in the Al–O film compared to the glass film. Furthermore, using an Al–O underlayer led to an improvement in the squareness of the hysteresis loop.
The Effect of Various Vehicles on the Naproxen Permeability through Rat Skin: A Mechanistic Study by DSC and FT-IR Techniques. (2016)

It is concluded that naproxen permeation through rat skin may be facilitated by utilizing the vehicle systems. Lipid fluidization and lipid extraction are among suggested mechanisms.
Effects of Passive Body Heating on the Sleep of Older Female Insomniacs (1996)

The purpose of this study was to evaluate passive body heating (PBH) as a treatment for insomnia in older adults. Polysomnographic recordings of older adults routinely show an increase in sleep fragmentation and a substantial decrease in slow-wave sleep (SWS) consistent with complaints of "lighter" more disturbed sleep. An increase in body temperature in young adults early in the evening by way of PBH has been shown to produce an increase in SWS in the early part of the sleep period. In a crossover design, nine female insomniacs (aged 60-72 yr) participated in two consecutive nights of PBH, involving hot (40-40.5 degrees C) and luke-warm (37.5-38.5 degrees C) baths 1.5 hours before bedtime. Significant improvement in sleep continuity and a trend toward an increase in SWS occurred after hot baths. Results of subjective measures showed that subjects experienced significantly "deeper" and more restful sleep after hot baths. In addition, hot baths resulted in a significant delay of temperature nadir in comparison to baseline nights.
Approaches to the Ideology Work for Students of Higher Learning in the New Era (2000)

Information based economy is a new type of economy. Ideological work for students of higher learning must serve to develop students of high quality with creative spirits. It must have the features of the spirit of our age, prediction, incorporation and clear objectives. The approach must be thought from the macro, medium and micro level. The macro level approach of ideological work must help to educate and shape students with correct theory so they will have correct political attitude. The medium level approach must help to provide a favourable condition for students with creative spirits by reform and innovation in the ideological work itself. The micro level approach helps to foster talents with creative spirits by teaching them the methods and approaches to cope with information based economy.
Hepatitis C Viral Infection in Liver Transplantation (1996)

Despite recurrence of HCV infection in most patients after transplantation, survival following primary orthotopic liver transplantation for chronic active hepatitis secondary to HCV infection remains favorable, and these patients should continue to be candidates for liver transplantation. In contrast, survival following retransplantation for HCV infection is poor and should be reconsidered. There is an apparent association between the intensity of immunosuppression and recurrent HCV infection and cirrhosis that warrants continued evaluation. Interferon therapy appears to afford benefit to patients in whom recurrent HCV hepatitis develops after transplantation.
Are acute infarcts the cause of leukoaraiosis? Brain mapping for 16 consecutive weeks (2014)

Neuroimaging of older adults commonly reveals abnormality (leukoaraiosis) in the cerebral white matter. Studies have established that extensive leukoaraiosis predicts dementia and disability, but the pathogenesis of leukoaraiosis remains unclear. We recruited 5 patients with leukoaraiosis and performed magnetic resonance mapping of the brain for 16 consecutive weeks. We observed tiny lesions arising de novo in the cerebral white matter. These lesions were clinically silent. They had the signature features of acute ischemic stroke. With time, the characteristics of these lesions approached those of pre-existing leukoaraiosis. Together, these findings suggest that tiny silent acute infarcts are a cause of leukoaraiosis.
Higher Expression of Toll-Like Receptors 2 and 4 in Blood Cells of Keratoconus Patiens (2017)

Inflammation may play a significant role in Keratoconus (KC), but the implication of immunity on this inflammatory response is unknown. Therefore, our aim was to determine the expression levels of Toll-like receptors 2 (TLR2) and 4 (TLR4) in monocytes and neutrophils from patients with KC and control subjects for demonstrating the role of innate immunity in KC. We also study the correlation between TLR2/TLR4 expression and serum levels of proinflammatory markers (IL-1β, IL-6, TNF-α, MMP-9 and NF-κB). Forty patients with bilateral KC (55% males; mean age; 33.1 ± 10.9 years) and 20 control subjects (55% males; mean age; 30.4 ± 7.6 years) were included in the study. Our results showed that mean expression of TLR2 and TLR4 in both neutrophils and monocytes was significantly higher in patients with KC compared to control subjects (all p < 0.0001). Furthermore, KC patients also showed higher serum levels of IL-1B, IL-6, TNF-α, MMP-9 (all p < 0.0001) and NF-κB (p = 0.036). In addition, we found a strong correlation between TLR2 expression in both monocytes and neutrophils (all p < 0.0001), and TLR4 in monocytes (all p < 0.05) with serum levels of IL-1B, IL-6, TNF-α and MMP-9. In conclusion, these findings suggest that TLRs may play an important role in the pathophysiology of KC.
Cytokines and the liver in health and disease. Effects on liver metabolism and fibrogenesis. (1995)

The liver is the largest organ in the body providing a large number of essential functions for the organism. It is the center for the metabolism of nutrients and drugs, and plays a key role in the unspecific immune system by harbouring Kupfer cells, the majority of all macrophages. The liver is the main site for the synthesis of many different metabolites and releases most of the plasma proteins. All these functions of the liver must be coordinated and regulated in response to metabolic changes and minor or major injuries. This is accomplished by metabolites, the autonomous nerve system, the endocrine system and by cytokines, which form a complex network of mediator molecules. Cytokines modulate liver metabolism in many ways. Synthesis of acute phase proteins is regulated by cytokines such as IL-1, IL-6, IL-11, leukemia inhibitory factor, TNF, transforming growth factor beta, epidermal growth factor, and ciliary derived neurotropic factor, which interact synergistically with corticosteroids and insulin. Hepatic lipid metabolism and hepatic carbohydrate metabolism are also regulated by cytokines and by classical hormones. Cytokines play an important role in the pathogenesis of liver diseases and liver fibrosis, which is the common morphological reaction after chronic injury of the liver. An uncontrolled production of extracellular matrix and its impaired degradation destroy the architecture of the liver and its function. Fat-storing cells (Ito cells, lipocytes, perisinusoidal cells) are the major source of extracellular matrix in the liver. They are activated to proliferate or to produce extracellular matrix compounds by cytokines like transforming growth factor beta, and platelet derived growth factor (PDGF). Interferon gamma and alpha inhibit this activation. Modulation of fibrogenesis by these cytokines may be helpful for future treatment of liver fibrosis.
Neonatal Livers: A Source for the Isolation of Good-Performing Hepatocytes for Cell Transplantation (2013)

Hepatocyte transplantation is an alternative therapy to orthotopic liver transplantation for the treatment of liver diseases. However, the supply of hepatocytes is limited given the shortage of organs available to isolate good-functioning quality cells. Neonatal livers may be a potential source alternative to adult livers to obtain good-performing hepatic cells for hepatocyte transplantation, which has not yet been explored profoundly. High-yield preparations of viable hepatocytes were isolated from 1- to 23-day-old liver donors, cryopreserved, and banked. Cell integrity and functional quality assessment were performed after thawing. Neonatal hepatocytes showed better postthawing recovery compared with adult hepatocytes, as shown by the viability values that did not differ significantly from freshly isolated cells, a higher expression of adhesion molecules (β1-integrin, β-catenin, and E-cadherin), better attachment efficiency, cell survival, and a lower number of apoptotic cells. The metabolic performance of thawed hepatocytes has been assessed by ureogenesis and drug-metabolizing capability (cytochrome P450 and UDP-glucuronosyltransferase enzymes). CYP2A6, CYP2C9, CYP2E1, and CYP3A4 activities were found in all cell preparations, while CYP1A2, CYP2B6, CYP2C19, and CYP2D6 activities were detected only in hepatocytes from a few neonatal donors. The expression of UGT1A1 and UGT1A9 (transcripts and protein) was detected in all hepatocyte preparations, while activity was measured only in some preparations, probably due to lack of maturity of the enzymes. However, isoforms UGT1A6 and UGT2B7 showed considerable activity in all preparations. Compared to adult liver, the hepatocyte isolation procedure in neonatal livers also provides thawed cell suspensions with a higher proportion of hepatic progenitor cells (EpCAM(+) staining), which could also participate in regeneration of liver parenchyma after transplantation. These results could imply important advantages of neonatal hepatocytes as a source of high-quality cells to improve human hepatocyte transplantation applicability.
ChemInform Abstract: REACTIONS OF 5‐BROMOURACILS AS ELECTRON ACCEPTORS. REDUCTIVE DEBROMINATION INVOLVING AN INITIAL ELECTRON TRANSFER PROCESS (1984)

Abstract Die S‐Bromuracile (I) reagieren mit N‐Methylindol (II) und N‐Methylphenothiazin (VII) unter reduktiverDebromierung zu den Uracilen (III).