gebhart/patrepeval huggingface dataset:
Piecing together cis‐regulatory networks: insights from epigenomics studies in plants (2017)

5-Methylcytosine, a chemical modification of DNA, is a covalent modification found in the genomes of both plants and animals. Epigenetic inheritance of phenotypes mediated by DNA methylation is well established in plants. Most of the known mechanisms of establishing, maintaining and modifying DNA methylation have been worked out in the reference plant Arabidopsis thaliana. Major functions of DNA methylation in plants include regulation of gene expression and silencing of transposable elements (TEs) and repetitive sequences, both of which have parallels in mammalian biology, involve interaction with the transcriptional machinery, and may have profound effects on the regulatory networks in the cell. Methylome and transcriptome dynamics have been investigated in development and environmental responses in Arabidopsis and agriculturally and ecologically important plants, revealing the interdependent relationship among genomic context, methylation patterns, and expression of TE and protein coding genes. Analyses of methylome variation among plant natural populations and species have begun to quantify the extent of genetic control of methylome variation vs. true epimutation, and model the evolutionary forces driving methylome evolution in both short and long time scales. The ability of DNA methylation to positively or negatively modulate binding affinity of transcription factors (TFs) provides a natural link from genome sequence and methylation changes to transcription. Technologies that allow systematic determination of methylation sensitivities of TFs, in native genomic and methylation context without confounding factors such as histone modifications, will provide baseline datasets for building cell-type- and individual-specific regulatory networks that underlie the establishment and inheritance of complex traits. This article is categorized under: Laboratory Methods and Technologies > Genetic/Genomic Methods Biological Mechanisms > Regulatory Biology.
Beyond communication training: The MaRIS model for developing medical students’ human capabilities and personal resilience (2019)

<b>Purpose:</b> Human capabilities in medicine, including communication skills, are increasingly important within the complex, challenging and dynamic landscape of healthcare. Supporting medical students to manage unavoidable role-related stressors adaptively may help mitigate the anguish that is too commonly reported among the profession. We developed a model, "MaRIS", underpinned by contemplative pedagogy, to support medical students to enhance their human capabilities, across all three domains of Bloom's taxonomy, and their personal resilience. It is the first to integrate Mindfulness, affective Reflection, Impactive experiences and a Supportive environment into medical curriculum design. Here, we describe the theoretical basis underpinning MaRIS and present a preliminary study to evaluate its impact on students' subjectively-rated capabilities.<b>Materials and Methods:</b> A questionnaire capturing self-ratings of competence, empathy and resilience, as well as impressions of their experiences, was administered to foundation year medical students before (T0), during (T1) and after delivery (T2).<b>Results:</b> Fifty-five students completed the survey at all time points. Mean scores for all domains increased significantly from T0 to T1 and from T0 to T2. Free-text comments suggest learning impact across the cognitive, psychomotor and affective domains.<b>Conclusions:</b> MaRIS appears to facilitate medical students' establishment of the foundations for building the human capabilities and personal resilience required for professional practice.
Contrast-free detection of myocardial fibrosis in hypertrophic cardiomyopathy patients with diffusion-weighted cardiovascular magnetic resonance (2015)

DW-CMR is sensitive to diffuse myocardial fibrosis and is capable of characterizing the extent of fibrosis in HCM patients.
Banqueros sobradamente preparados: algunas reflexiones sobre la idoneidad para el desempeño (2014)

El desarrollo de una empresa es una tarea compleja. Cuando el objeto del negocio es financiero, ademas de todas las dificultades formales compartidas con el resto de sociedades de capital, las complicaciones aumentan formidablemente, dada la especialidad de la materia y la responsabilidad del gestor no solo frente a los socios sino tambien ante los depositantes y la sociedad en general. Por ello, es natural que en el nuevo y revisado sistema financiero las exigencias para pilotar un proyecto de esta indole sean crecientes, debiendo reunir el consejero o directivo determinados perfiles que confirmen su pertinencia e idoneidad para el desempeno. El flamante estatus viene definido por normativa europea y espanola �tanto financiera como tradicional (Ley de Sociedades de Capital)� y por los estandares tecnicos bosquejados por la Autoridad Bancaria Europea.
Isolation of hemopoietic precursor cells from human marrow by negative selection using monoclonal antibodies and immunomagnetic beads. (1990)

A purified or enriched population of human marrow hemopoietic precursor cells may be produced by using a monoclonal antibody to specifically bind to the desired cell population (positive selection), or by removing undesired cell populations contaminating the mixture (negative selection). We describe a simple negative selection technique using commercially available monoclonal antibodies and immunomagnetic beads to produce a mononuclear marrow cell fraction which comprises no more than 2% of the initial cell population yet contains all the BFU-E and CFU-GM present originally. This procedure is an efficient method for producing hemopoietic precursor cells appropriate for studying cell-substrate adhesions, homing mechanisms, and response to growth factors without interference by contaminating accessory cells.
Chemistry of tropan-3-yl ethers. Part III. Kinetic properties of tropan-3α-yl methanesulphonate (1971)

The reactions of tropan-3α-yl methanesulphonate with various nucleophiles have been investigated. Rate constants of the displacement reactions have been correlated with the configuration at C-3 of the reaction products. For stronger nucleophiles SN2 type reactions predominate over SNI type, and more β-substituted tropanes are produced than α-isomers.
Neutron Radii Determinations from the Ratio ofπ−Elastic Scattering fromC12,13andO16,18 (1979)

Differential elastic cross-section ratios and absolute cross sections have been measured for $^{12,\phantom{\rule{0ex}{0ex}}13}\mathrm{C}$ at 29.2-and 49.5-MeV average ${\ensuremath{\pi}}^{\ensuremath{-}}$ energy and for $^{16,\phantom{\rule{0ex}{0ex}}18}\mathrm{O}$ at 29.2 MeV. Range telescopes detected the scattered pions. The ratio data were compared with different optical-potential calculations to extract neutron radii of 2.35 \ifmmode\pm\else\textpm\fi{} 0.03 fm for $^{13}\mathrm{C}$ and 2.81 \ifmmode\pm\else\textpm\fi{} 0.03 fm for $^{18}\mathrm{O}$, relative to the neutron radii of $^{12}\mathrm{C}$ (2.31) and $^{16}\mathrm{O}$ (2.60), respectively. Our studies indicate little sensitivity to the optical model used.
ChemInform Abstract: The High‐Pressure SNAr Reaction of N‐p‐Fluorobenzyl‐2‐chlorobenzimidazole with Amines; an Approach to Norastemizole and Analogues. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Efficient calibration for robust indoor localization based on low-cost BLE sensors (2019)

The fingerprinting method for indoor localization is associated with a high calibration effort. In order to improve the efficiency of this time-consuming method, a new calibration procedure is proposed. It allows to reduce the calibration effort associated with fingerprinting considerably and ensures a sufficient accuracy at the same time. The proposed procedure takes into account the geometry of the environment subject to study, in order to empirically estimate the distribution of the signal strength at predefined positions. Thus, mobile sensor localization can be carried out robustly and the deviation from real position is less than 0.75 m in about 90% of the cases. This is achieved even though the calibration effort is reduced by almost 84% compared to the original one.
Data from Association of Pathologic Complete Response with Long-Term Survival Outcomes in Triple-Negative Breast Cancer: A Meta-Analysis (2023)

&lt;div&gt;Abstract&lt;p&gt;Pathologic complete response (pCR) following neoadjuvant therapy has been associated with improved event-free survival (EFS) and overall survival (OS) in early-stage breast cancer. The magnitude of this association varies by breast cancer subtype, yet further research focusing on subtype-specific populations is limited. Here we provide an updated and comprehensive evaluation of the association between pCR and survival outcomes in triple-negative breast cancer (TNBC). A literature review identified neoadjuvant studies, including clinical trials, real-world cohort studies, and studies that pooled multiple trials or cohorts, which reported EFS/OS results by pCR in patients with early-stage TNBC. Meta-analyses were performed to evaluate the association between pCR and EFS/OS and to predict long-term survival outcomes based on pCR status. Sensitivity analyses were conducted to assess the impact of cross-study variations. Twenty-five studies with over 4,000 patients with TNBC were identified. A synthesis of evidence from these studies suggested substantial improvement in EFS and OS for pCR versus non-pCR [EFS HR (95% confidence interval): 0.24 (0.20–0.29); OS: 0.19 (0.15–0.24)]; consistent results were reported in sensitivity analyses. Collectively, our findings suggest that adjuvant therapy is associated with improved EFS/OS in patients with TNBC who received neoadjuvant therapy, regardless of pCR status.&lt;/p&gt;&lt;/div&gt;
Translational efficiency of phi X174 lysis gene E is unaffected by upstream translation of the overlapping gene D reading frame (1990)

The lysis gene E of bacteriophage phi X174 is entirely embedded in gene D. Expression studies of genes D and E in Escherichia coli minicells and lysis times obtained in the presence or absence of D translation showed that the simultaneous expression of gene D does not affect protein E production. Thus, unlike other overlapping gene pairs, gene E expression is independent from the upstream translation of gene D. lacZ fusion studies and primer extension inhibition analysis (toeprinting) revealed an intrinsically weak E ribosome-binding site, which seems to be the major factor determining the low expression rate of the gene and thus proper scheduling of cell lysis.
In Vivo DNA Electrotransfer for Immunotherapy of Cancer and Neurodegenerative Diseases (2013)

Electroporation is the process commonly referred to the transient increase in the permeability of cell membranes on submission to electric field pulses. Electroporation has become an increasingly extensive method to enhance in vivo DNA delivery for both gene therapy applications as well as for delivery of DNA vaccines, mostly against cancer. In vivo gene electrotransfer is of special interest since it is the most efficient non-viral strategy for gene delivery and it is worthy of low manufacturing costs, ease of realization and favorable safety profile. No adverse findings observed in toxicology and biodistribution/integration studies have been warranted for the evaluation of this approach in humans. Therefore, gene delivery followed by electroporation is currently being investigated in several clinical trials. The positive outcomes of early studies suggest that the efficacy of gene delivery and immunogenicity has greatly improved by electroporation. This review briefly summarizes salient features and recent findings that have contributed to the rapid progress of electroimmunotherapy as well as an overview of advanced clinical studies in oncology. Translation of in vivo DNA electrovaccination for neurodegenerative diseases as well as future expectations are also discussed.
Final Report and Documentation for the PLD11 Multipurpose Programmable Logic VME Board Design (1999)

The PLD11 board is a 9U VME board containing 11 Altera 10K100 Programmable Logic Devices, controlled impedance clock tree, VME interface, programming inteface, 0C3 (155 Mbps) interface and serial port. The 11 Altera 10K100 Programmable Logic Devices arranged to provide four 96 bit wide buses for a total of 384 parallel digital data lines in and out of the board that can operate up to 100 Mhz for a aggrigate throughput of 38.4 Gpbs. The 14.44 X 15.75 board has over 1.1 million programmable gates that can be programmed through a serial interace. The board contains a clock reference and 50 ohm clock distribution tree that can drive each of the eleven 10K100 devices with two critically timed clock references. Five external clock references can be used to drive five additional PLD 11 boards for a total of six boards operating all from the same synchronous clock reference. A system of six boards provides just under 7 million programmable gates.
Structural characterization of a PCP–R didomain from an archaeal nonribosomal peptide synthetase reveals novel interdomain interactions (2021)

Nonribosomal peptide synthetases (NRPSs) are multimodular enzymes that produce a wide range of bioactive peptides, such as siderophores, toxins, and antibacterial and insecticidal agents. NRPSs are dynamic proteins characterized by extensive interdomain communications as a consequence of their assembly-line mode of synthesis. Hence, crystal structures of multidomain fragments of NRPSs have aided in elucidating crucial interdomain interactions that occur during different steps of the NRPS catalytic cycle. One crucial yet unexplored interaction is that between the reductase (R) domain and the peptide carrier protein (PCP) domain. R domains are members of the short-chain dehydrogenase/reductase family and function as termination domains that catalyze the reductive release of the final peptide product from the terminal PCP domain of the NRPS. Here, we report the crystal structure of an archaeal NRPS PCP-R didomain construct. This is the first NRPS R domain structure to be determined together with the upstream PCP domain and is also the first structure of an archaeal NRPS to be reported. The structure reveals that a novel helix-turn-helix motif, found in NRPS R domains but not in other short-chain dehydrogenase/reductase family members, plays a major role in the interface between the PCP and R domains. The information derived from the described PCP-R interface will aid in gaining further mechanistic insights into the peptide termination reaction catalyzed by the R domain and may have implications in engineering NRPSs to synthesize novel peptide products.
Real-life vs. standard driving cycles and implications on EV power electronic reliability (2016)

This work studies the impact of drive cycles on the power semiconductor temperature, and consequently on the reliability figures. The paper quantifies the reliability errors stemming from the use of standard mission profiles by comparing them to data sets using real-life high-resolution mission profiles. It is found that standard drive cycles underestimate the stress on the power semiconductors. The paper thus proposes a new and more realistic Drive Cycle for Reliability Assessment (DCRA) to be used for lifetime estimation of power electronic components.
LEIOMYOMA OF THE CARDIO ‐ OESOPHAGEAL JUNCTION WITH OVERLYING SQUAMOUS CELL CARCINOMA OF THE OESOPHAGUS IN ASSOCIATION WITH A HIATAL HERNIA (1961)

SUMMARY The case history of a 72 year old woman with a leiomyoma of the cardio‐oesophageal junction associated with an overlying squamous cell carcinoma, the combined tumour lying in a hiatal hernia, is presented. The treatment was by resection, alimentary continuity being restored by oesophagogastrostomy. The importance of oesophagoscopy in all suspected cases of oesophageal neoplasia including leiomyoma is stressed. Where the mucosa overlying an extramucosal tumour is normal, biopsy is contraindicated because of interference with subsequent surgical enucleation. When, however, the mucosa is in any way abnormal biopsy is mandatory, as in the case described an early squamous cell carcinoma was revealed. A brief review of the literature with particular reference to the symptomatology and radiological findings is included.
Predictive Maintenance Practices For Operational Safety of Battery Energy Storage Systems. (2021)

Abstract not provided.
Measurement of Modulation frequency Responses of Semiconductor Laser Diodes with a Frequency Sweeper Based on Delayed Self-Heterodyne Technique (1996)

Intrinsic modulation frequency responses of semiconductor laser diodes (LD's) are measured by using a novel frequency sweeper as the light source of the active-layer photomixing method. The modulation frequency responses of LD's up to 8GHz are measured avoiding the influences of the parasitic impedances.The frequency sweeper developed here employs only one distributed feedback LD, and is almost insensitive to ambient temperature fluctuations. Moreover, a high speed control of the beat frequency is possible, and the implementation of the lock-in detection is easily done with a suitable modulation pattern.
Highly Reactive Multilayer‐Assembled TiO2 Coating on Electrospun Polymer Nanofibers (2008)

Highly efficient photocatalytically active TiO2-coated polymer fibers are prepared using a facile and universal method involving layer-by-layer assembly of TiO2 nanoparticles and POSS molecules on various electrospun fibers. The TiO2-coated fibers display excellent photocatalytic properties in degradation of allyl alcohol under UV illumination, without degradation of substrates.
Adaptive multiobjective optimisation for energy efficient interference coordination in multicell networks (2014)

In this paper, the authors investigate the distributed power allocation for the multicell orthogonal frequency division multiple access networks by taking both the energy efficiency and the intercell interference (ICI) mitigation into account. A performance metric termed as throughput contribution is exploited to measure how the ICI is effectively coordinated. To achieve a distributed power allocation scheme for each base station (BS), the throughput contribution of each BS to the network is first given based on a pricing mechanism. Different from the existing works, a biobjective problem is formulated based on the multiobjective optimisation theory, which aims at maximising the throughput contribution of the BS to the network and minimising its total power consumption at the same time. By using the method of the Pascoletti and Serafini scalarisation, the relationship between the varying parameters and the minimal solutions is revealed. Furthermore, to exploit the relationship an algorithm is proposed based on which all the solutions on the boundary of the efficient set can be achieved by adaptively adjusting the involved parameters. With the obtained solution set, the decision maker has more choices in the power allocation schemes in terms of both the energy consumption and the throughput. Finally, the performance of the algorithm is assessed by the simulation results.
Infusion-related events during natalizumab: No need for post-infusion monitoring (2019)

This retrospective cohort study assessed the timing of infusion-related adverse events (IAEs) during natalizumab (NTZ) administration in well-documented relapsing-remitting multiple sclerosis (RRMS) patients who had received NTZ infusions in our centre between 2006 and 2018. In 225 RRMS patients (14,174 NTZ infusions), 276 IAEs (1.95%) occurred in 60 patients. All documented severe IAE occurred during infusion. Of the 19 moderate adverse events, 17 were during infusion. None of the reactions that occurred after the infusion required intervention. These results suggest that post-infusion monitoring is not necessary in patients who do not have an adverse event during infusion.
Flavonol Triglycosides from the Leaves of Silphium Albiflorum (2006)

In the course of chemotaxonomic studies of the genus Silphium two new and six known flavonol glycosides were isolated from the leaves of S. albiflorum. The structures of the new flavonoids were established by spectral analysis ( 1 H NMR, 13 C NMR, HMQC, HMBC, ROESY, TOCSY, MS) as isorhamnetin 3-O-α-L-rhamnosyl (1′“→6″)-O-β-D-galactopyranoside 7-O-β-D-apiofuranoside (1) and quercetin 3-O-α-L-rhamnosyl (1′“→6″)-O-β-D-galactopyranoside 7-O-β-D-apiofuranoside (2). LC-MS and LC-MS n (with post-column manganese complexation) were applied to elucidate the structures of some of the studied compounds.
Coronenetetraimide‐Centered Cruciform Pentamers Containing Multiporphyrin Units: Synthesis and Sequential Photoinduced Energy‐ and Electron‐Transfer Dynamics (2015)

A series of coronenetetraimide (CorTIm)-centered cruciform pentamers containing multiporphyrin units, in which four porphyrin units are covalently linked to a CorTIm core through benzyl linkages, were designed and synthesized to investigate their structural, spectroscopic, and electrochemical properties as well as photoinduced electron- and energy-transfer dynamics. These systems afforded the first synthetic case of coroneneimide derivatives covalently linked with dye molecules. The steady-state absorption and electrochemical results indicate that a CorTIm and four porphyrin units were successfully characterized by the corresponding reference monomers. In contrast, the steady-state fluorescence measurements demonstrated that strong fluorescence quenching relative to the corresponding monomer units was observed in these pentamers. Nanosecond laser flash photolysis measurements revealed the occurrence of intermolecular electron transfer from triplet excited state of zinc porphyrins to CorTIm. Femtosecond laser-induced transient absorption measurements for excitation of the CorTIm unit clearly demonstrate the sequential photoinduced energy and electron transfer between CorTIm and porphyrins, that is, occurrence of the initial energy transfer from CorTIm (energy donor) to porphyrins (energy acceptor) and subsequent electron transfer from porphyrins (electron donor) to CorTIm (electron acceptor) in these pentamers, whereas only the electron-transfer process from porphyrins to CorTIm was observed when we mainly excite porphyrin units. Finally, construction of high-order supramolecular patterning of these pentamers was performed by utilizing self-assembly and physical dewetting during the evaporation of solvent.