gebhart/patrepeval huggingface dataset:
Combination of fluorescence in situ hybridization and scanning force microscopy for the ultrastructural characterization of defined chromatin regions (1996)

Although the internal arrangement of interphase chromatin is still a matter of conjecture, there exists a large body of evidence for the compartmentalization of chromosomal domains. A study based on combined scanning force and optical microscopy of supramolecular chromatin spreads produced by isotonic lysis of cells suspended in phosphate-buffered saline has been conducted. The ultrastructure of fluorescent labeled chromosomes was resolved with the topographical contrast provided by the scanning force microscope. Fluorescence in situ hybridization was used to label specific DNA sequences. The location of different pericentromeric chromosome regions was determined by fluorescence microscopy and correlated with scanning force microscope topography. Using a single DNA probe, discrimination between labeled chromosome pairs of an aneuploid cell was possible, based on the different intensities of fluorescence signals. The results show that the in situ hybridization technique with fluorescence labeling is compatible with scanning force microscopy. The combination of these methods can be used for the specific identification and lateral localization of DNA sequences in spread chromatin, opening the possibility for the ultrastructural characterization of defined genes in the scanning force microscope.
Switched tank converter based partial power architecture for voltage regulation applications (2018)

Switched capacitor converters and their resonant alternatives (e.g., switched-tank converters) have been shown to provide high efficiency and high power density. One major problem of these converters is poor voltage regulation capability. This paper proposes a partial power architecture based on the switched-tank converters to realize the voltage regulation. This topology can provide high efficiency, high power density, and can be potentially used in applications with wide voltage and power ranges. A prototype of a 48-to-12 V 500 W converter was designed and built to demonstrate the advantages of this topology, achieving a peak efficiency of 98.4% and a power density of ~800 W/in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> .
Structures and stabilities of α-hetero-substituted organolithium and organosodium compounds. Energetic unimportance of d-orbital effects (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructures and stabilities of α-hetero-substituted organolithium and organosodium compounds. Energetic unimportance of d-orbital effectsPaul V. R. Schleyer, Timothy Clark, Alexander J. Kos, Gunther W. Spitznagel, Cornelia Rohde, Dorit Arad, K. N. Houk, and Nelson G. RondanCite this: J. Am. Chem. Soc. 1984, 106, 22, 6467–6475Publication Date (Print):October 1, 1984Publication History Published online1 May 2002Published inissue 1 October 1984https://pubs.acs.org/doi/10.1021/ja00334a001https://doi.org/10.1021/ja00334a001research-articleACS PublicationsRequest reuse permissionsArticle Views754Altmetric-Citations298LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Structural Revision and Biomimetic Synthesis of Goupiolone B (2017)

Goupiolones A and B are unique phenolic compounds with significant DNA-damaging activity. In this study, the structure of goupiolone B was revised on the basis of DFT calculations of the <sup>13</sup> C NMR chemical shifts and biosynthetic considerations. The dibenzobicyclo[3.2.2]nonane skeleton of the revised structure suggested that goupiolone B was produced by oxidative coupling between catechol and goupiolone A, which was strongly supported by biomimetic synthesis. Furthermore, racemization of goupiolone B was observed during the attempted resolution of its racemic mixture. A plausible racemization mechanism involving α-ketol rearrangement is proposed.
Functional genomics by mass spectrometry (2000)

Systematic analysis of the function of genes can take place at the oligonucleotide or protein level. The latter has the advantage of being closest to function, since it is proteins that perform most of the reactions necessary for the cell. For most protein based ('proteomic') approaches to gene function, mass spectrometry is the method of choice. Mass spectrometry can now identify proteins with very high sensitivity and medium to high throughput. New instrumentation for the analysis of the proteome has been developed including a MALDI hybrid quadrupole time of flight instrument which combines advantages of the mass finger printing and peptide sequencing methods for protein identification. New approaches include the isotopic labeling of proteins to obtain accurate quantitative data by mass spectrometry, methods to analyze peptides derived from crude protein mixtures and approaches to analyze large numbers of intact proteins by mass spectrometry directly. Examples from this laboratory illustrate biological problem solving by modern mass spectrometric techniques. These include the analysis of the structure and function of the nucleolus and the analysis of signaling complexes.
Central venous catheter placement in children: evaluation of electrocardiography using J‐wire (1999)

Misplacement of a central venous catheter may lead to myocardial perforation and dysrhythmia. Atrial electrocardiography (ECG) through a saline column is an effective but complex method to determine the accurate location of catheters. We evaluated a simplified variant of this technique using the guidewire as an internal electrode in 23 children (5-16 years old) undergoing spinal surgery. Catheters were placed using a Seldinger technique after jugular or subclavian venous puncture. Each time the operator recognized the atrial signal, the catheter was found to be correctly placed on the chest radiograph (20/23). In three patients, the atrial signal was not obtained. A technical error was responsible in one case whereas the two others were related to aberrant migrations of the catheter either into a subclavian vein or into the pleura. In this latter case, the complication was unrecognized on the first radiograph despite malposition having been predicted by atrial ECG. We conclude that a method using atrial ECG guidance is sensitive and specific, and may be an alternative to the classical chest radiograph to detect accurate placement of central venous catheters in children.
Impairment of Heme Oxygenase Expression in Immuno-suppressed Mice Exacerbates Ischemic Heart Myocyte Cell Death: Reversible by Bilirubin and Restoration of Nitric Oxide (2015)

Background: Cardiovascular risk and therapy inimmuno-deficient patients is a challenge for cardiologists. Nitric oxide (NO) plays a central role in vascular response to ischemicdamage by decreasing microvascular resistance and is an important regulator of the immune system. The aim of this study was to assess theefficacy of restoration of HO-1 and whether the increase in bilirubin levelsattenuates cardiac remodeling in immuno-suppressed mice. Method: Myocardial infarction was inducedby LAD ligation in immunosuppressive (BALB SCID) mice. Mice comprised 4 groups: sham, MI, MItreated with the HO-1 inducer CoPP with and without HO activity inhibitor, SnMP.Results: Mice with MI had increasedlevels of inflammatory cytokines, myocardial fibrosis and myocyte death (p<0.05)as compared to control animals. Left ventricle end diastolic area (EDA) wasreduced and fractional area change (FAC) and angiogenesis was increased(p<0.01) in CoPP-treated mice compared to the MI group. Inflammation,cardiac iNOS expression, myocardial cell death and fibrosis were reduced inCoPP treated animals as a result of increased bilirubin levels (p<0.05)serum nitrite levels were increased. Foxp3 (marker of T regulatory cells), pAMPK, pAKT expression in cardiac tissues wasincreased in CoPP-treated animals. SnMP reversed these positive cardiaceffects. Conclusion: This novelstudy suggests a critical role of HO-1 –mediated increase in bilirubin inimproving cardiac function and microvascular tone control after onset of MI inimmuno-suppressed mice through the modulation of nitric oxide synthaseexpression.
TIME‐GATED FLUORESCENCE SPECTROSCOPY OF THE TUMOR LOCALIZING FRACTION OF HpD IN THE PRESENCE OF CATIONIC SURFACTANT (1989)

Time-gated fluorescence spectroscopy was performed on the tumor localizing fraction (TLF) of HpD in buffer at different concentrations of cationic surfactant. This technique obtains emission spectra with programmable delay relative to the excitation pulse. According to the measured fluorescence decay-time constants (approximately 0.7, approximately 3 and approximately 15 ns) three gates were considered, delayed by 0, 5 and 18 ns, respectively, to evaluate the contribution of the emitting molecular species to the spectra. Simultaneous to these measurements, fluorescence decay waveforms and time-integrated spectra were also detected. In buffer and in detergent micelles the fluorescence spectra are given by the superposition of the emission of the different molecular species present in the solution, and no appreciable interaction among the chromophores is observed. On the contrary, in the pre-micellar range of the surfactant, evidence for the existence of an energy transfer mechanism was found. This effect seems to be related to the configurational state of the TLF polymeric chains and depends on the relative TLF/surfactant concentration.
Transport vs. Deep Neural Networks in OAM Underwater Communications (2020)

Signal attenuation, resulting in low signal to noise ratio (SNR), in underwater optical communications (UWOC) is a problem that degrades classification performance. We develop and contrast several novel ways to create machine learning (ML) and optimal transport-based attenuation models and insert these models in a convolutional neural network (CNN) classification training pipeline. We show that including these ML-based attenuation models in the CNN classifier training, significantly improves classification performance.
Current concepts review: vascularized free bone transplants. (1981)

Current concepts review: vascularized free bone transplants. A Weiland; The Journal of Bone & Joint Surgery
DPYDGenotyping to Predict Adverse Events Following Treatment With Fluorouracil-Based Adjuvant Chemotherapy in Patients With Stage III Colon Cancer (2016)

eudract Identifier 2005-003463-23.
Changes in monocyte expression of CD54 (ICAM-1), UPDOSE protocol (2011)

<b>Copyright information:</b>Taken from "Inhalation of Ultrafine Particles Alters Blood Leukocyte Expression of Adhesion Molecules in Humans"Environmental Health Perspectives 2005;114(1):51-58.Published online 20 Sep 2005PMCID:PMC1332656.This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI. In this and following figures, data are shown as mean ± SE changes from baseline. Nominal UFP exposure concentrations are shown in μg/m. Exposure, = 0.012.
Perceived Need for Care and Health Care Utilization Among Depressed and Anxious Patients With and Without Suicidal Ideation (2012)

Our results validate previous findings about perceived needs and health-care use of persons with SI. The results also suggest that suicidal persons are more seriously ill, and that they need more professional care, dedication, and specialized expertise than anxious and depressed persons without SI, especially in the domains of information and referral.
Pressure Dependence of Ionic Conductivity in KCl, NaCl, KBr, and NaBr (1972)

The pressure dependence of the ionic conductivity of KCl, NaCl, KBr, and NaBr has been measured to 6 kbar in the intrinsic and extrinsic temperature regions. In the intrinsic region the conductivity deviates markedly from a simple exponential decrease with pressure. A detailed analysis, including contributions from anions and extrinsic cation vacancies, shows that pressure can suppress the intrinsic vacancies sufficiently to cause such a deviation. The analysis also shows that the uncertainties in the estimated activation volumes are greater than those determined in previous work. For KCl, NaCl, KBr, and NaBr crystals, respectively, the estimated activation volumes for motion of cation vacancies are 8 \ifmmode\pm\else\textpm\fi{} 1, 7 \ifmmode\pm\else\textpm\fi{} 1, 11 \ifmmode\pm\else\textpm\fi{} 1, and 8 \ifmmode\pm\else\textpm\fi{} 1 ${\mathrm{cm}}^{3}$/mole, and the activation volumes for formation of Schottky defects are 61 \ifmmode\pm\else\textpm\fi{} 9, 55 \ifmmode\pm\else\textpm\fi{} 9, 54 \ifmmode\pm\else\textpm\fi{} 9, and 44 \ifmmode\pm\else\textpm\fi{} 9 ${\mathrm{cm}}^{3}$/mole. The values for activation volumes of motion agree with predictions of the strain-energy model of Keyes. Schottky-defect-formation volumes are substantially larger than the molar volumes in KCl and NaCl, but in KBr and NaBr they are approximately equal.
Deferral Aims to Deter Chagas' Parasite (1991)

REJECTING prospective blood donors who are at risk of carrying the Chagas' disease parasite may help prevent the spread of the potentially fatal disease in the United States without significantly reducing blood supplies, even in communities that have a large number of immigrants from areas of Latin America where the disease is endemic. This is the conclusion of a study by the Los Angeles County—University of Southern California Hospital Blood Bank that was presented in November at the annual meeting of the American Association of Blood Banks, held in Los Angeles. The study describes the effects of a donor screening policy adopted last year after one case of transfusion-associated Chagas' disease was reported in California and one in New York, NY (<i>JAMA</i>. 1989;262:1433). The blood bank's policy calls for the questioning of prospective donors who were born in or who traveled to areas of Latin America where Chagas' disease is
Deterministic mechanisms of pattern formation (2011)

In this appendix we provide a mathematical description of the three major deterministic models of self-organized pattern formation that are commonly invoked to explain mechanisms of spatial self-organization in the biogeosciences. In these models patterns emerge from a mechanism of symmetry-breaking instability, whereby the uniform state of the system becomes unstable, thereby leading to the emergence of spatial patterns. Spatial interactions induce this instability, whereas the resulting patterns are stabilized by suitable nonlinear terms. In Turing and kernel-based models, symmetry breaking is the result of the interactions between short-range activation and long-range inhibition, i.e., of positive and negative feedbacks acting at different spatial scales. In the third class of models (i.e., differential-flow models) symmetry breaking emerges as a result of the differential-flow rate between two (or more) species.
Fault-tolerance thresholds for the surface code with fabrication errors (2017)

The construction of topological error correction codes requires the ability to fabricate a lattice of physical qubits embedded on a manifold with a nontrivial topology such that the quantum information is encoded in the global degrees of freedom (i.e., the topology) of the manifold. However, the manufacturing of large-scale topological devices will undoubtedly suffer from fabrication errors---permanent faulty components such as missing physical qubits or failed entangling gates---introducing permanent defects into the topology of the lattice and hence significantly reducing the distance of the code and the quality of the encoded logical qubits. In this work we investigate how fabrication errors affect the performance of topological codes, using the surface code as the test bed. A known approach to mitigate defective lattices involves the use of primitive swap gates in a long sequence of syndrome extraction circuits. Instead, we show that in the presence of fabrication errors the syndrome can be determined using the supercheck operator approach and the outcome of the defective gauge stabilizer generators without any additional computational overhead or use of swap gates. We report numerical fault-tolerance thresholds in the presence of both qubit fabrication and gate fabrication errors using a circuit-based noise model and the minimum-weight perfect-matching decoder. Our numerical analysis is most applicable to two-dimensional chip-based technologies, but the techniques presented here can be readily extended to other topological architectures. We find that in the presence of $8%$ qubit fabrication errors, the surface code can still tolerate a computational error rate of up to $0.1%$.
Frontotemporal dementia with ubiquitinated neuronal inclusions and visuospatial impairment (2005)

Dementia with ubiquitinated neuronal inclusions has been described only with frontotemporal dementia (FTD). The authors report a patient with progressive FTD accompanied by prominent impairments in visuospatial cognitive functions. Pathology was characterized by ubiquitin-positive intranuclear and cytoplasmic neuronal inclusions. Cortical pathology was widespread and posteriorly accentuated but spared the hippocampal dentate gyrus.
Motor Bus Transfer System Performance Testing and the Search for a New Transfer Success Criterion (2016)

The petrochemical industry presently has no industry standards on the performance requirements for relays used to supervise critical process motor bus transfers (MBTs). A device testing protocol is proposed, and the results of extensive performance testing of relays used to implement the fast and in-phase methods of a motor bus synchronous transfer are analyzed. The existing industry criteria for determining the success of a completed transfer are used to evaluate these test results. The development of digital MBT systems with data recording capabilities provided a means of recording transfer data and provides key insight into what happens during actual transfers. Case studies of a number of live MBTs are presented and analyzed to begin to determine if a new transfer metric can be derived, based on recorded transfer inrush current and power when transfer is completed.
Workers' compensation for skin disease. (1994)

The concept of workers' compensation is not new; in fact, ancient sources make reference to it. Following an historical overview of compensation systems, the author discusses the compensation plans now in effect in the United States, Canada, and other countries. The physician's role also is described, with an emphasis on how to assess an impairment and write the report.
Impact of Alkyl Spacer Length on Aggregation Pathways in Kinetically Controlled Supramolecular Polymerization (2015)

We have investigated the kinetic and thermodynamic supramolecular polymerizations of a series of amide-functionalized perylene bisimide (PBI) organogelator molecules bearing alkyl spacers of varied lengths (ethylene to pentylene chains, PBI-1-C2 to PBI-1-C5) between the amide and PBI imide groups. These amide-functionalized PBIs form one-dimensional fibrous nanostructures as the thermodynamically favored states in solvents of low polarity. Our in-depth studies revealed, however, that the kinetic behavior of their supramolecular polymerization is dependent on the spacer length. Propylene- and pentylene-tethered PBIs follow a similar polymerization process as previously observed for the ethylene-tethered PBI. Thus, the monomers of these PBIs are kinetically trapped in conformationally restricted states through intramolecular hydrogen bonding between the amide and imide groups. In contrast, the intramolecularly hydrogen-bonded monomers of butylene-tethered PBI spontaneously self-assemble into nanoparticles, which constitute an off-pathway aggregate state with regard to the thermodynamically stable fibrous supramolecular polymers obtained. Thus, for this class of π-conjugated system, an unprecedented off-pathway aggregate with high kinetic stability could be realized for the first time by introducing an alkyl linker of optimum length (C4 chain) between the amide and imide groups. Our current system with an energy landscape of two competing nucleated aggregation pathways is applicable to the kinetic control over the supramolecular polymerization by the seeding approach.
Engineering Education in the Age of Autonomous Machines (2021)

We advocate creating a cross-disciplinary program that gives students a sufficient technical background and includes a capstone project to create a real-world autonomous machine from scratch by applying the knowledge learned in classes.
Establishing the correspondence between closed contours of objects in images with projective distortions (2018)

In this paper, we consider the task of finding the correspondence between closed contours of objects in an image pair with small projective distortions. Several methods are considered and their comparison is performed. The experiments results for two contour sets are provided. Sufficient conditions of the applicability of the method of selecting the nearest contour are represented and proven.
The Nature of Enzymes (2001)

This chapter contains sections titled: Nomenclature: Enzymes as Catalysts Enzymes as Proteins Catalysis and Mechanism Practical Enzymology: Purification, Estimation of Purity References
The Invisible Tyranny of the Mexican Media: Tlatelolco and Beyond (2009)

The aim of the study was to compare the quality of breast aspirates obtained by two methods of fine needle aspiration cytology (FNAC): the auto-vacuum device and the syringe pistol holder, using flow cytometry. Both techniques were used in 44 fresh surgical specimens. Subsequently, these specimens were fixed and mounted in paraffin. Both the aspirates and the de-waxed histology material were prepared for flow cytometry using a FACScan. Flow cytometry showed that the means for DNA index and S-phase fraction were similar for aspirates obtained with both techniques. Mean aneuploidy was significantly higher in the auto-vacuum device aspirates than in the surgical specimen (43.4 SD +/- 23 vs. 27.9 SD +/- 17; p = 0.04). Mean DNA index and S-phase fraction were similar in cells from aspirates and surgical specimens. It is concluded that both FNAC methods are of similar efficacy in collecting aspirates for flow cytometry.