gebhart/patrepeval huggingface dataset:
Proteomic analysis of human low‐density lipoprotein reveals the presence of prenylcysteine lyase, a hydrogen peroxide‐generating enzyme (2009)

Abstract The molecular mechanisms underlying the relationship between low‐density lipoprotein (LDL) and the risk of atherosclerosis are not clear. Therefore, detailed information on the protein composition of LDL may help to reveal its role in atherogenesis. Liquid‐phase IEF has been used to resolve LDL proteins into well‐defined fractions on the basis of p I , which improves the subsequent detection and resolution of low abundance proteins. Besides known LDL‐associated proteins, this approach revealed the presence of proteins not previously described to reside in LDL, including prenylcysteine lyase (PCL1), orosomucoid, retinol‐binding protein, and paraoxonase‐1. PCL1, an enzyme crucial for the degradation of prenylated proteins, generates free cysteine, isoprenoid aldehyde and hydrogen peroxide. Addition of the substrate farnesylcysteine to lipoprotein resulted in a time‐dependent generation of H 2 O 2 which was stronger in very low density lipoprotein (VLDL) than in LDL or HDL, reflecting the greater protein content of PCL1 in VLDL. Farnesol, a dead end inhibitor of the PCL1 reaction, reduced H 2 O 2 generation by VLDL. PCL1 is generated along with nascent lipoprotein, as shown by its presence in the lipoprotein secreted by HepG2 cells. The finding that an enzyme associated with atherogenic lipoproteins can itself generate an oxidant suggests that PCL1 may play a significant role in atherogenesis.
Withdrawal Notice: Beauty and Wellness Turn Towards a Green Economy (2021)

: Beauty and wellness remain for both women and men an important target also because global attractiveness is considered a cue indicating the ability to maintain beauty and wellness. Consequently neuromarketing has occurred as an innovative mean to interact with consumer's trend, actually looking for high-quality and natural cosmetics and food. By the biometric studies, in fact, it seems possible to verify the consumers’ willingness to buy the right products they are looking for. On the other hand, customers are requesting cosmeceuticals and nutraceuticals acting positively on the superficial appearance of face and body, seem to be able to boost the immune system also, limiting the stress conditions. This general disease is induced by the actual way of living on a planet invaded by a great quantity of waste released from human’s activities. The consequent pollution alters the ecological balance of the body, influencing negatively the life of animals and plants also. Thus, the induced stress, further increased by the COVID-19 spread, could change the human brain' neural conditions. Consequently, in humans the production of both neurons' stem cells and cortisol could decrease, while in plants, the patterns of growth could affect the leaf cuticle and stomatal conductance. Thus brain, under psychosocial stress, actively demands energy from the body, eliciting a marked increase in eating by a major carbohydrate intake which sacrifices the requirements of other organs. In any way, pollution has wide-ranging adverse health and social effects, further worsened by an increased worldwide aged population who, requiring more food and goods, increases the waste production. What the proposed solution? To change the way of consuming and producing, it is necessary to optimize the material resource, minimizing waste that should be recycled and reused by a green economy approach. So doing, it could be possible to realize a new industrial Renaissance reducing consumption of water, energy and natural raw materials. Thus the necessity to use sustainable technologies and materials for maintaining human wellbeing in an environment in which the species' biodiversity could be assured. At this purpose, chitin nanofibrils(CN), nano-lignin (NL) and their derived compounds have been proposed to make nanoparticles (NPs) by the use of water as solvent. These NPs, loaded with different active ingredients, may be embedded into innovative natural or man-made polymers and used to make biodegradable carriers in the form of non-woven tissues and films. Depending on the polymers and on the actives loaded into the fibers, these new vehicles may be used to produce smart cosmeceuticals, nutraceuticals or advanced medications. It is interesting to underline that all these innovative NPs and tissue/films can be produced by natural polymers and ingredients, obtained from waste materials and produced by sustainable technologies at low consumption of water and energy, according to the new green economy.
Diversity of early life-history traits in freshwater eels and the evolution of their oceanic migrations (2014)

Early life-history traits of all 19 anguillid eel species and subspecies were examined to help understand the evolutionary processes of their oceanic migrations in comparison with their migration distances and the geography of their species ranges. Tropical species were found to have fewer myomeres, greater body depths, higher growth rates, shorter larval durations, and smaller maximum larval sizes than temperate species. The relationships among larval characteristics such as growth rate, age at metamorphosis, and maximum larval size differed among tropical and temperate species and corresponded with the maximum latitudes of their species ranges. Temperate eel leptocephali with slow growth and large maximum size with slender bodies appear to be specialized for long migrations and dispersal over a wide range of distances to higher latitudes, while having flexible sizes of metamorphosis and recruitment. Tropical species with faster growth metamorphose earlier at a relatively fixed size, which would facilitate larval retention near their species ranges at low latitudes. Changes in the early life-history traits of tropical eels appear to have occurred during the evolution of longer migrations as they entered temperate regions.
The Impact of Intermittent Fasting (Ramadan Fasting) on Psoriatic Arthritis Disease Activity, Enthesitis, and Dactylitis: A Multicentre Study (2019)

Intermittent circadian fasting, namely Ramadan, is a common worldwide practice. Such fasting has a positive impact on psoriasis, but no data exist on its role in psoriatic arthritis (PsA)-a disease that is clearly linked to body mass index. We enrolled 37 patients (23 females and 14 males) with a mean age 43.32 ± 7.81 and they fasted for 17 h for one month in 2016. The baseline PsA characteristics were collected and 12 (32.4%) patients had peripheral arthritis, 13 (35.1%) had axial involvement, 24 (64.9%) had enthesitis, and 13 (35.1%) had dactylitis. Three patients (8.1%) were treated with methotrexate, 28 (75.7%) with TNF-α blockers, and 6 (16.2%) with IL-17 blockers. After a month of intermittent fasting, C-reactive protein (CRP) levels decreased from 14.08 ± 4.65 to 12.16 ± 4.46 (<i>p</i> < 0.0001), Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) decreased from 2.83 ± 1.03 to 2.08 ± 0.67 (<i>p</i> = 0.0078), Psoriasis Area Severity Index (PASI) decreased from 7.46 ± 2.43 to 5.86 ± 2.37 (<i>p</i> < 0.0001), and Disease Activity index for PSoriatic Arthritis (DAPSA) decreased from 28.11 ± 4.51 to 25.76 ± 4.48 (<i>p</i> < 0.0001). Similarly, enthesitis improved after fasting, with Leeds Enthesitis Index (LEI) decreasing from 2.25 ± 1.11 to 1.71 ± 0.86 (<i>p</i> < 0.0001) and dactylitis severity score (DSS) decreasing from 9.92 ± 2.93 to 8.54 ± 2.79 (<i>p</i> = 0.0001). Fasting was found to be a predictor of a decrease in PsA disease activity scores (DAPSA, BASDAI, LEI, DSS) even after adjustment for weight loss. IL-17 therapy was found to be an independent predictor of decreases in LEI after fasting. These preliminary data may support the use of chronomedicine in the context of rheumatic diseases, namely PsA. Further studies are needed to support our findings.
Contract law in the cases: 2018 in review - part 1 of 3 (2019)

The unconscionable conduct provisions in the Australian Consumer Law (ACL) and in the ASIC Act represent one of the most significant reforms to commercial law since the introduction of the misleading and deceptive conduct regime in the Trade Practices Act of 1974.
Changing Effect of i.c.v. IL‐1β on Vasopressin Release in Anaesthetized, Female Rats at Different Stages of Lactation: Role of Prostaglandins and Noradrenaline (1996)

Interleukin-1 beta stimulates oxytocin and vasopressin release in conscious, male rats and causes a rise in blood pressure. These experiments were done to : A) examine the effect of i.c.v. interleukin-1 beta (1 ng/microliter) on circulating levels of vasopressin in female rats at different stages of lactation and B) determine if alpha-adrenergic mechanisms and/or prostaglandins were involved as mediators. Urethane-anaesthetized nonlactating rats and rats at Day 7, 10, 20 and 26 of lactation were set up for arterial blood sampling and i.c.v. injections. One mL blood samples were obtained in one min periods before, and at 1, 2.5, 5, 10, 30, 60 and 120 min after the following treatments: i.c.v. treatment with either interleukin-1 beta (1 ng in 1 microliter PBS-BSA) or PBS-BSA (1 microliter) as a vehicle control; or i.c.v. treatment with interleukin-1 beta following pretreatment with either phentolamine (1.7 micrograms/microliter i.c.v.) or indomethacin (1 microgram/microliter i.c.v.). As blood was sampled, isotonic saline was infused (1 mL per min) and blood pressure was monitored to minimize any hypovolemic effects due to sampling. Extracted plasma was assayed using a specific vasopressin radioimmunoassay. Interleukin-1 beta i.c.v. stimulated the release of vasopressin above that elicited by PBS-BSA alone in non-lactating rats resulting in an approximate 1.2 to 2-fold increase in plasma hormone levels. Throughout the first half of lactation, vasopressin responsiveness to i.c.v. interleukin-1 beta treatment was markedly attenuated. In latter stages of lactation, the response recovered and resembled that of non-lactators around the time of weaning. Prostaglandins consistently mediate a stimulatory action of interleukin-1 beta on vasopressin release whereas alpha-adrenergic mechanisms mediate a depression of interleukin-1 beta-induced vasopressin release during the early to middle stages of lactation. It is possible that the depression in interleukin-1 beta-stimulation of vasopressin release in early to mid-lactation is conducive for nursing to occur and that the increase in vasopressin responsiveness towards the latter stages of lactation represents a component of the weaning process.
A dual watermarking technique for images (1999)

Article A dual watermarking technique for images Share on Authors: Saraju P. Mohanty Dept. of Electrical Engg., Indian Institute of Science, Bangalore - 560 012, India Dept. of Electrical Engg., Indian Institute of Science, Bangalore - 560 012, IndiaView Profile , K. R. Ramakrishnan Dept. of Electrical Engg., Indian Institute of Science, Bangalore - 560 012, India Dept. of Electrical Engg., Indian Institute of Science, Bangalore - 560 012, IndiaView Profile , Mohan Kankanhalli School of Computing, National University of Singapore, Kent Ridge, Singapore - 119 260 School of Computing, National University of Singapore, Kent Ridge, Singapore - 119 260View Profile Authors Info & Claims MULTIMEDIA '99: Proceedings of the seventh ACM international conference on Multimedia (Part 2)October 1999 Pages 49–51https://doi.org/10.1145/319878.319891Published:01 October 1999 42citation1,228DownloadsMetricsTotal Citations42Total Downloads1,228Last 12 Months9Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Today's Phytoremediation: Success Had Led to Growth (1999)

(1999). Today's Phytoremediation: Success Had Led to Growth. International Journal of Phytoremediation: Vol. 1, No. 2, pp. 111-113.
P‐141: A Wide‐view Multi‐domain Vertical‐alignment Liquid Crystal Display with High Transmittance and High Contrast (2006)

Abstract A wide viewing angle multi‐domain vertical‐alignment liquid crystal display (VA‐LCD) is proposed. According to this design, by integrating a kind of wide‐view circular polarizer, a contrast ratio higher than 800:1 is predicted over the entire ±85 ° viewing cone with the transmittance higher than 33% for an eight‐domain VA‐LCD. Potential application for LCD television is emphasized.
Long term activation in a 15 MeV radiotherapy accelerator (2008)

The high energy electrons produced by linear accelerators can generate several radioisotopes by means of photonuclear reactions. The activity produced in the head of a 15 MV Mevatron Siemens 77 medical linear accelerator was measured by high resolution gamma-ray spectrometry, during its decommissioning 1 year after the last clinical use. The activity of 54Mn, 57Co, 60Co, 181W, and 65Zn was measured while the activity of other radioisotopes which emit soft beta or gamma rays such as 59Ni, 63Ni, and 55Fe was inferred by appropriate scaling factors. The number of pieces requiring particular care is limited, their mass does not exceed some tens of kilograms, and their volume is of the order of some thousands of cm3. Moreover, these materials are metals and the emitted radiation can be easily shielded, so that storage should not create particular problems.
O2‐05–04: Hyperphosphorylation of Tau induces local polyproline II helix in the proline‐rich domain (2008)

The major constituent of the neurofibrillary tangles of Alzheimer's disease is a hyperphosphorylated form of the microtubule-binding protein tau. Hyperphosphorylation of tau, which occurs significantly within the proline-rich domain, is associated with structural and functional changes that are poorly understood. Understanding the relationship between hyperphosphorylation of tau and structural changes in tau is a central question in the mechanism of neurofibrillary tangle formation. Peptides and larger protein constructs derived from the proline-rich region of tau as well as other tau domains were synthesized with defined phosphrylation states. The non-phosphorylated and phosphorylated peptides and proteins were analyzed by NMR and circular dichroism in order to identify the specific local changes in the structure of tau that are induced by hyperphosphorylation. Phosphorylation of serine and threonine residues throughout the proline-rich domain of tau induced structural changes from a disordered conformation to a type II polyproline helix. The largest phosphorylation-induced structural changes were observed in tau peptides derived from residues 174–183 or residues 229–238. Phosphorylation-induced changes to polyproline helix were also observed in tau peptides incorporating residues 211–219. Phosphorylation of tau peptides derived from tau residues 196–209 resulted in the disruption of a nascent α-helix. In these peptides, phosphoserine and phosphothreonine residues exhibited ordered values of phi (φ) (3JαN = 3.1–6.2 Hz; mean = 4.7 Hz) compared to non-phosphorylated serine and threonine, indicating specific conformational restriction upon phosphorylation. Phosphorylation within the proline-rich domain did not affect the cis-trans isomerization equilibrium of the peptides examined, suggesting that phosphorylation-associated cis-trans isomerism is due to long-range interactions. As the proline-rich domains are linker regions between hydrophobic and aggregation-prone domains of tau, these results suggest that global reorganization of tau may occur upon hyperphosphorylation of proline-rich domain in tau. These results provide insight into possible mechanisms of phosphorylation-dependent structural changes of tau in Alzheimer's disease. Hyperphosphorylation within the proline-rich domain of tau can induce specific local structural changes from a disordered conformation to an ordered polyproline II helix conformation.
Single-cell dynamics of core pluripotency factors in human pluripotent stem cells (2022)

Summary The human transcription factors OCT4, SOX2, and NANOG form a core signaling network critical for maintaining stem cell pluripotency and self-renewal potential. The spatiotemporal expression dynamics of these pluripotency factors throughout differentiation is unclear, limiting our understanding of stem cell fate decisions. Here, we combined CRISPR/Cas9-mediated gene editing with microraft array technology to generate human embryonic stem cell lines with endogenously tagged fluorophores for OCT4, SOX2, and NANOG. Fluorescence time-lapse imaging revealed that pluripotent stem cells show gastrulation-like patterning without direct chemical induction. Directed differentiation to the three primary germ layers—endoderm, mesoderm, and ectoderm—revealed distinct spatiotemporal patterns of SOX2 and NANOG expression in single cells. Finally, we captured dynamic changes in cell morphology during ectoderm differentiation corresponding to the formation of neural rosettes. This study provides a robust method for generating live-cell reporters in human stem cells and describes the single-cell dynamics of human pluripotency factors during differentiation.
Laparoscopic Approaches to Uterine Leiomyomas (2001)

Northwestern University Medical School, Chicago, Illinois Correspondence: Magdy P. Milad, MD, MS, Northwestern University Medical School, 333 East Superior Street, Suite 1564, Chicago, IL 60611. E-mail: mmilad@ nmh.org.
New Stable and Persistent Acyclic Diaminocarbenes (2015)

The portfolio of acyclic diaminocarbenes (ADACs) has been substantially expanded, owing to the synthesis of eleven new formamidinium salts, mostly of the type [(iPr2N)CH(NRR')][PF6], for use as immediate carbene precursors. The corresponding ADACs (iPr2N)C(NRR') were sufficiently stable for isolation in the case of NRR' = 2-methylpiperidino (13), 3-methylpiperidino (14), 4-methylpiperidino (15), morpholino (17) and NiPrPh (20), but had to be trapped in situ in the case of NRR' = 2,2,6,6-tetramethylpiperidino (12) and NiPrMe (19). The tetraaryl-substituted ADACs (Ph2N)2C (22) and (Ph2N)C[N(C6F5)2] (24) also could only be generated and trapped in situ. Trapping with elemental selenium was particularly efficient, affording the corresponding selenourea derivative in all cases, whereas trapping with [{Rh(μ-Cl)(cod)}2] did not work for 12 and 24. The (77)Se NMR chemical shifts, δ((77)Se), of the selenourea compounds derived from the new ADACs lie in the range 450-760 ppm, which indicates a much higher electrophilicity and π-accepting capability of ADACs in comparison with NHCs, which typically exhibit δ((77)Se)<200 ppm. The extreme low-field shift of 758 ppm observed for 12Se can be rationalised by the results of DFT calculations, which revealed that ADAC 12 has a minimum energy conformation with the 2,2,6,6-tetramethylpiperidino unit perpendicular to the N2C plane, which suppresses the π donation of this amino group and causes an unusually low LUMO energy and high electrophilicity.
Five batches representative of Ontario-grown American ginseng root produce comparable reductions of postprandial glycemia in healthy individualsThis article is one of a selection of papers published in this special issue (part 1 of 2) on the Safety and Efficacy of Natural Health Products. (2007)

Evidence indicates that the glycemia-lowering effect of American ginseng root may be batch dependent. We therefore evaluated the effect of 5 root batches, representative of Ontario-grown American ginseng, on postprandial glucose and insulin indices. Twelve healthy subjects (5 male, 7 female), mean +/- SE age 26.5 +/- 2 years, body mass index 23.96 +/- 3.41 kg/m2, fasting blood glucose 4.77 +/- 0.04 mmol/L, were assigned to consume 9 g of American ginseng from 5 farms (A-E), administered in randomized sequence on 5 separate visits, and a water-control during the 6th and last visit. Treatments were consumed 40 min before a 2-hour 75-gram oral glucose tolerance test. Plasma glucose and insulin were measured at baseline, before, and during the test. Compared with control, batches A and C reduced glucose incremental area under the curve (IAUC) by 35.2% (156 vs. 240 mmol.min/L) and 32.6% (162 vs. 240 mmol.min/L), respectively. Batches A, C, and E reduced incremental peak glucose by 1.3, 1.2, and 1.1 mmol/L, respectively. Batch C reduced the insulin IAUC by 27.7% (15.8 vs. 21.8 nmol.min/L). Effects on glucose and insulin parameters were not different across ginseng treatments. The mean of the 5 ginseng treatments reduced peak postprandial glucose by 1.0 mmol/L, glucose IAUC by 27.7% (173 vs. 240 mmol.min/L), and insulin IAUC by 23.8% (16.6 vs. 21.8 nmol.min/L) relative to control. (All results statistically significant at p < 0.05.) American ginseng decreased postprandial glycemia and insulinemia; however, 40% of the batches did not reduce glycemia with the anticipated magnitude, irrespective of their saponin composition.
Association of Lack of Speech Arrest During Cortical Stimulation With Interhemispheric Reorganization of the Functional Language Network in Patients With Brain Tumors (2023)

<b>BACKGROUND.</b> Brain tumors induce language reorganization, which may influence the extent of resection in surgical planning. Direct cortical stimulation (DCS) allows definitive language mapping during awake surgery by locating areas of speech arrest (SA) surrounding the tumor. Although functional MRI (fMRI) combined with graph theory analysis can illustrate whole-brain network reorganization, few studies have corroborated these findings with DCS intraoperative mapping and clinical language performance. <b>OBJECTIVE.</b> We evaluated whether patients with low-grade gliomas (LGGs) without SA during DCS show increased right-hemispheric connections and better speech performance compared with patients with SA. <b>METHODS.</b> We retrospectively recruited 44 consecutive patients with left perisylvian LGG, preoperative language task-based fMRI, speech performance evaluation, and awake surgery with DCS. We generated language networks from ROIs corresponding to known language areas (i.e., language core) on fMRI using optimal percolation. Language core connectivity in the left and right hemispheres was quantified as fMRI laterality index (LI) and connectivity LI on the basis of fMRI activation maps and connectivity matrices. We compared fMRI LI and connectivity LI between patients with SA and without SA and used multivariable logistic regression (<i>p</i> < .05) to assess associations between DCS and connectivity LI, fMRI LI, tumor location, Broca area and Wernicke area involvement, prior treatments, age, handedness, sex, tumor size, and speech deficit before surgery, within 1 week after surgery, and 3-6 months after surgery. <b>RESULTS.</b> Patients with SA showed left-dominant connectivity; patients without SA lateralized more to the right hemisphere (<i>p</i> < .001). Between patients with SA and those without, fMRI LI was not significantly different. Patients without SA showed right-greater-than-left connectivity of Broca area and premotor area compared with patients with SA. Regression analysis showed significant association between no SA and right-lateralized connectivity LI (<i>p</i> < .001) and fewer speech deficits before (<i>p</i> < .001) and 1 week after (<i>p</i> = .02) surgery. <b>CONCLUSION.</b> Patients without SA had increased right-hemispheric connections and right translocation of the language core, suggesting language reorganization. Lack of interoperative SA was associated with fewer speech deficits both before and immediately after surgery. <b>CLINICAL IMPACT.</b> These findings support tumor-induced language plasticity as a compensatory mechanism, which may lead to fewer postsurgical deficits and allow extended resection.