gebhart/patrepeval huggingface dataset:
BTN2A, a New Immune-Checkpoint Targeting Vg9Vd2 T Cell Cytotoxicity (2019)

Background: Anti-tumoral response of Vg9Vd2 T cells requires sensing of phosphoantigens accumulated in malignant cells through binding of butyrophilin 3A(BTN3A). Moreover, an unknown partner located in human Chr6 was shown to be mandatory to BTN3A-mediated Vg9Vd2 T cell activation in murine models. Here, we identified butyrophilin 2A (BTN2A), which is located to Chr6, as a requirement for BTN3A-mediated Vg9Vd2 T cell cytotoxicity against cancer cells. Methods: CRISPR-Cas9-mediated inactivation of BTN2A1/2A2 isoforms was performed in Daudi, K562 and HEK-293T cells. Vg9Vd2 T cells expanded from healthy PBMCs were co-cultured with wild-type or BTN2AKO cells +/- BrHPP (1 µM), HMBPP (0.1 µM) or zoledronate (45 µM), or anti-BTN2A mAb, and Vg9Vd2 T cell degranulation (%CD106ab+ cells), and intracellular TNFa and IFNg assessed after 4h. Mouse T cell hybridoma 53/4 expressing TCRVg9Vd2-MOP were co-cultured overnight with NIH3T3 murine fibroblasts transfected with BTN3A- and/or BTN2A-encoding plasmids +/-HMBPP(10 µM), or increasing doses of HMBPP or anti-BTN3 20.1 mAb. BTN2A transcript expression in normal vs. tumoral tissue was analyzed using GEPIA tool. Anti-BTN2A mAb staining was performed on human samples of primary AML, cervical and pancreatic carcinoma and assessed by flow cytometry. Results: Degranulation and intracellular IFNg/TNFa (n=6) were abolished in Vg9Vd2 T cells co-cultured with BTN2AKO Daudi, K562 and HEK-293T cells compared to wild-type, in all conditions tested including anti-BTN3 20.1. Murine cells do not express no BTN2A1 or BTN3A orthologs and are unable to activate human Vg9Vd2 T cells. Ectopic expression of BTN2A and BTN3A combination but neither BTN2A or BTN3A alone in murine NIH3T3 cells, allows triggering of IL-2 secretion in mouse 53/4-TCRVg9Vd2-MOP reporter cells in presence of HMBPP or 20.1 mAb in dose-dependent manner. Anti-BTN2A mAb was able to suppress Vg9Vd2 T cell degranulation/cytokine secretion against cancer cell lines and activation of mouse 53/4-TCRVg9Vd2-MOP reporter by BTN2A/BTN3A-expressing NIH3T3 in a dose-dependent manner. BTN2A transcript was significantly up-regulated in pancreatic, ovarian and cervical carcinoma vs. normal tissue. Extracellular BTN2A protein was detected in primary hematological and solid tumors. Conclusion: Here, we show that BTN2A is mandatory for BTN3A-mediated function in human Vg9Vd2 T cells. Moreover, concomitant BTN2A and BTN3A expression empowers murine T cells with activation through Vg9Vd2 TCR, opening new roads for mouse models of Vg9Vd2 T cell anti-tumoral responses. We describe an anti-BTN2A able to suppress Vg9Vd2 T cell function, and we show BTN2A expression in primary tumors. These results are relevant for understanding Vg9Vd2 T cell antitumoral immunity triggered by phosphoantigens and amino-bisphosphonates. Disclosures Olive: ImCheck Therapeutics: Consultancy, Equity Ownership, Patents &amp; Royalties; GlaxoSmithKline: Patents &amp; Royalties.
Photothermal bleaching of nickel dithiolene for bright multi-colored 3D printed parts (2023)

HP's Multi Jet Fusion is a powder bed fusion 3D printing technology that utilizes a carbon-based radiation absorber in combination with a near infrared (NIR) light source to facilitate the fusion of polymer powder in a layer-by-layer fashion to generate 3D parts. Most available carbon-based and NIR radiation absorbers have an intrinsic dark color, which as a result will only produce black/gray and dark colored parts. However, there are many applications that require variable color, including prosthetics, medical models, and indicators, among others. To create white, bright colored, and translucent parts with MJF, a visibly transparent and colorless radiation absorber is required. In this paper, we designed an activating fusing agent (AFA) that contains a red, strong NIR absorbing dye that turns colorless after harvesting irradiation energy during the MJF 3D printing process and provide a bright colored part when working with other color agents.
ÉLABORATION ET CARACTERISATION DE FILMS MINCES D'OXYDE D'INDIUM DOPE A L'ETAIN OBTENUS PAR VOIE SOL-GEL Potentialité pour la réalisation d'électrodes sur silicium poreux (2003)

L'oxyde d'indium dopé à l'étain (ITO) présente une bonne transparence dans le visible et une importante conductivité électrique. Ce travail est consacré à l'étude des performances de films minces multicouches d'ITO obtenus par la voie sol-gel. La microscopie électronique en transmission (MET) a permis d'analyser morphologie et structure, et a mis en évidence pour la première fois la structure complexe de tels films sol-gel. La MET et l'analyse par rétrodiffusion de particules a ont précisé la corrélation entre la densité des multicouches, l'apparition de croûtes intermédiaires et la conductivité électrique résultante. Ce travail valide l'utilisation du recuit rapide pour la densification des films sol-gel en comparaison avec un traitement classique. Nous démontrons la potentialité de la voie sol-gel pour la croissnce d'électrodes d'ITO sur silicium poreux (SP). Les structures ITO/SP, pour lesquelles la photoluminescence du SP est partiellement préservée, ont été étudiées en terme de diode Schottky
High-speed liquid-crystal modulators using transient nematic effect (1989)

The transient nematic effect, together with multiple pass technique and optimal temperature effect, was employed to demonstrate high-speed liquid-crystal modulators. As a result, nematic liquid-crystal modulators with frame time less than 50 μs have been achieved in the visible spectral region.
Enhanced thin film analysis via High Resolution RBS using the NEC CARBS system (2022)

Advancements in thin film deposition techniques can now produce films of only a few monolayers in thicknesses, with multiple applications emerging in the nano technology field. This maturing manufacturing technique is driving the need for diagnostics tools able to accurately measure depth profiles. To meet this need, the Compact Automated Rutherford Back-Scattering (CARBS) system is under development at National Electrostatics Corp. (NEC) for nanometer thin film analysis using High-resolution RBS (HRBS) within a 4 x 4meter footprint. We present the recent development of the system and demonstrate a study of HRBS applied to a 30nm CoAl alloy film. We compare the performance of the CARBS system with the conventional NEC HRBS end station and discuss the advantages of HRBS over SIMS method.
Avoiding menstruation: a review of health and lifestyle issues. (2004)

This article (1) reviews the decades-long history of short-term dosing regimens delaying the onset of expected, spontaneous menses or withdrawal bleeding in oral contraceptive users up to 20 days; (2) outlines treatment schedules that suppress menstrual bleeding for several months; and (3) evaluates the recently approved extended dosing regimen of 3 months' duration. For single-term postponement of normal menses, estrogen-progestogen combinations can be employed, starting about 7 days after ovulation. Oral contraceptive users can skip the 7-day pill-free period and continue with the active pills in the next package. The main focus of this review is the development of extended dosing schedules that result in cycles lasting 7 weeks up to several months and reduce the number of periods of bleeding and menstrual discomfort. Recently a dosing schedule was introduced into clinical use consisting of ethinyl estradiol, 30 microg, plus levonorgestrel, 150 microg/d, for 84 days, followed by 7 days of placebo. The pregnancy rate was < 1% for compliant women and 1.5% for all participants. A monophasic 21 + 7-day combination using the same daily doses had slightly higher pregnancy rates. The discontinuation rate for unscheduled bleeding and spotting was higher with extended dosing than with the conventional, 21 + 7 schedule.
FluidMeter (2018)

Water is the most vital nutrient in the human body accounting for about 60% of the body weight. To maintain optimal health, it is important for humans to consume a sufficient amount of fluids daily. Therefore, tracking the amount of human daily fluid intake has a myriad of health applications like dehydration prevention. In this paper, we present FluidMeter: a ubiquitous and unobtrusive system to track the amount of fluid intake leveraging the inertial sensors embedded in smartwatches. To achieve this, FluidMeter first separates the drinking activities from other human activities (playing, running, eating, etc.). Thereafter, it analyzes the sampled sensors data during the extracted drinking episodes to recognize the sequence of micro-activities (lift the bottle, sip, release the bottle) that constitute the drinking activity. Finally, it applies some machine learning algorithms on some features extracted from sampled sensor data during the sipping period to gauge the amount of fluid intake in the designated drinking episode. FluidMeter is evaluated by collecting more than 260 hours of different human activities by 70 different participants using different smartwatch models. The results show that FluidMeter can recognize the drinking activity and its micro-activities accurately which is comparable to that achieved by the state-of-the-art techniques. Finally, FluidMeter can estimate the overall amount of fluid intake in grams accurately with a estimation error limited to 15%, highlighting its promise as a ubiquitous health service.
The Human Computer in Flight Control (1957)

It is the unique decision-making ability which makes the human operator particularly suited to accomplish flight control computation. His decisions range from the simplest repetitive determination of control actions, each intended to minimize the error, to complex problems of the sequential reprogramming of all the lower levels of decision which, in totality, comprise his information transduction process. The human sensory-input and motor-output channels must be properly used in order to insure maximum efficiency of the over-all human computer. The perceived noise can only be separated from the signal after the decision-making operation to be performed has been clearly identified. Human noise filter and information handling rate characteristics may be enhanced through the utilization of inherent qualities of the various input and output channels. A brief summary is made of the considerable effort which has been devoted toward the development of mathematical models for human tracking. It is emphasized that the human decision-making ability must be incorporated into the model before a realistic representation can be attained. A stochastic model is considered which is constructed in a heuristic manner, incorporating the decision-making ability. Specific direction is indicated which may yield the most fruitful approach to the mathematical analysis of human flight control computation. This future model would be of great value as a link between the related work in the fields of neurophysiology, biophysics, and psychology. Most important, it should provide a cockpit synthesis technique which will aid the design of future manned aircraft.
COMPARISON OF DIFFERENT METHODS FOR THE DETERMINATION OF BREATH ALCOHOL (1981)

Five different methods for determining breath alcohol concentrations have been studied in our company: (1) alcotest tubes are inexpensive and easy to handle, they need however some experience for correct reading the test result and give only yes/no results. (2) electrochemical cells are limited in their accuracy in quick repetition of tests but they exhibit good long term stability. (3) devices comprising semiconductor gas sensors are appropriate for repetitive tests, because of reduced long term stability they need calibration in regular intervals. (4) gas chromatography is capable of excluding interference from vapours other than ethanol, though they are restricted to desk top size and need external carrier gas supply. (5) infrared absorption is an ideal measuring principle both for screening and evidential applications because of high accuracy and the possibility of tracking the breath alcohol concentration and on-line determination of the presence of alveolar air. The devices, however, have desk top size and are comparatively expensive. Methods (1) to (3) are appropriate for roadside screening tests, each under different conditions, (4) for evidential tests in police stations, and (5) for both precise screening and evidential tests. Optimum control of alcohol intoxication requires different test devices suiting the test situation. (TRRL)
Age-related clonal hematopoiesis (2017)

Age-related alterations in the human blood system occur in B cells, T cells, cells of the innate system, as well as hematopoietic stem and progenitor cells (HSPCs). Interestingly, age-related, reduced genetic diversity can be identified at the stem cell level and also independently in B cells and T cells. This reduced diversity is most probably related to somatic mutations or to changes in the microenvironmental niche. Either process can select for specific clones or cause repeated evolutionary bottlenecks. This review discusses the age-related clonal expansions in the human HSPC pool, which was termed in the past age-related clonal hematopoiesis (ARCH). ARCH is defined as the gradual, clonal expansion of HSPCs carrying specific, disruptive, and recurrent genetic variants, in individuals without clear diagnosis of hematological malignancies. ARCH is associated not just with chronological aging but also with several other, age-related pathological conditions, including inflammation, vascular diseases, cancer mortality, and high risk for hematological malignancies. Although it remains unclear whether ARCH is a marker of aging or plays an active role in these various pathophysiologies, it is suggested here that treating or even preventing ARCH may prove to be beneficial for human health. This review also describes a decision tree for the diagnosis and follow-up for ARCH in a research setting.
Additively Manufactured Parametric Universal Clip-System: An Open Source Approach for Aiding Personal Exposure Measurement in the Breathing Zone (2020)

Design for additive manufacturing is adopted to help solve problems inherent to attaching active personal sampler systems to workers for monitoring their breathing zone. A novel and parametric 3D printable clip system was designed with an open source Computer-aided design (CAD) system and was additively manufactured. The concept was first tested with a simple clip design, and when it was found to be functional, the ability of the innovative and open source design to be extended to other applications was demonstrated by designing another tooling system. The clip system was tested for mechanical stress test to establish a minimum lifetime of 5000 openings, a cleaning test, and a supply chain test. The designs were also tested three times in field conditions. The design cost and functionalities of the clip system were compared to commercial systems. This study presents an innovative custom-designed clip system that can aid in attaching different tools for personal exposure measurement to a worker’s harness without hindering the operation of the worker. The customizable clip system opens new possibilities for occupational health professionals since the basic design can be altered to hold different kinds of samplers and tools. The solution is shared using an open source methodology.
Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery (2020)

Cell therapy for the injured spinal cord will rely on combined advances in human stem cell technologies and delivery strategies. Here we encapsulate homotypic spinal cord neural stem cells (scNSCs) in an alginate-based neural ribbon delivery platform. We perform a comprehensive in vitro analysis and qualitatively demonstrate graft survival and injury site retention using a rat C4 hemi-contusion model. Pre-configured neural ribbons are transport-stable modules that enable site-ready injection, and can support scNSC survival and retention in vivo. Neural ribbons offer multifunctionality in vitro including co-encapsulation of the injury site extracellular matrix modifier chondroitinase ABC (chABC), tested here in glial scar models, and ability of cervically-patterned scNSCs to differentiate within neural ribbons and project axons for integration with 3-D external matrices. This is the first extensive in vitro characterization of neural ribbon technology, and constitutes a plausible method for reproducible delivery, placement, and retention of viable neural cells in vivo.
Breaking the spatial resolution barrier via iterative sound-light interaction in deep tissue microscopy (2012)

Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay exponentially with depth. Optical imaging beyond the ballistic regime has been demonstrated by hybrid techniques that combine light with the deeper penetration capability of sound waves. Deep inside highly scattering media, the sound focus dimensions restrict the imaging resolutions. Here we show that by iteratively focusing light into an ultrasound focus via phase conjugation, we can fundamentally overcome this resolution barrier in deep tissues and at the same time increase the focus to background ratio. We demonstrate fluorescence microscopy beyond the ballistic regime of light with a threefold improved resolution and a fivefold increase in contrast. This development opens up practical high resolution fluorescence imaging in deep tissues.
Cellular cholesterol efflux is modulated by phospholipid-derived signaling molecules in familial HDL deficiency/Tangier disease fibroblasts (2001)

Familial HDL deficiency (FHD) is the heterozygous form of Tangier disease (TD). Mutations of the ABCA1 gene cause FHD and TD. FHD/TD cells are unable to normally efflux cholesterol onto nascent HDL particles, which are rapidly catabolized. TD fibroblasts have an abnormal pattern of PLC and PLD activation following cell stimulation with HDL(3) or apolipoprotein A-I (apoA-I). We examined cellular cholesterol efflux in FHD and TD fibroblasts by phospholipid-derived-molecules, compared with that of normal cells. We used the PKC agonist 1,2-dioctanoylglycerol (DOG) and phorbol myristate acetate (PMA) to activate PKC, calphostin C, and GO 6976, as inhibitors of PKC; phosphatidic acid (PA), which is the product of PLD, and lysophosphatidic acid (LPA), phosphatidylcholine, sphingomyelin, and beta-cyclodextrin to investigate their potential effect in modulating cellular cholesterol efflux in [(3)H]cholesterol-labeled and cholesterol-loaded fibroblasts. Phosphatidylcholine, sphingomyelin, and beta-cyclodextrin promoted cholesterol efflux in an identical fashion in control, FHD, or TD fibroblasts. In a dose-dependent fashion, DOG (0-200 microM) increased apoA-I-mediated cellular cholesterol efflux by 40% in controls, 71% in FHD, and 242% in TD cells. PMA similarly increased cholesterol efflux to a maximum of 256% in controls, 182% in FHD, and 191% in TD cells. This effect was inhibited by calphostin C. PA (100 microM) also increased cholesterol efflux by 25% in control, 44% in FHD, and 100% in TD cells. Conversely, LPA reduced cholesterol efflux in a dose-dependent fashion in control and FHD cells (-50%, 200 microM) but not in TD cells, where efflux was increased by 140%. Propranolol (100 microM) significantly increased cholesterol efflux at 24 h in all three cell lines. n-Butanol partially decreased the DOG-mediated increase in cholesterol efflux. The inhibitory effect of calphostin C on DOG-stimulated cholesterol efflux could be partially overcome by propranolol, suggesting that PA is a downstream mediator of PKC-stimulated cholesterol efflux. We conclude that PLC and PLD activities are required for apoA-I-mediated cellular cholesterol efflux, and modulating cellular PA concentration can correct, at least partially, the cholesterol efflux defect in FHD and TD.
60-MHz wavelength-encoded tomography (WET) (2015)

Wavelength-encoded tomography (WET) is upgraded to a triple-time-lens system to perform ultrafast cross-sectional imaging through 68.4x-temporal magnification. 60-MHz A-scan rate is demonstrated by imaging a glass sample with 180-μm axial resolution.
Raman scattering and isotopic phonon effects in dodecaborides (2011)

The Raman spectra of numerous dodecaborides have been measured on high-quality single crystals at ambient conditions with high spectral resolution and signal-to-noise ratio. Besides the strong Raman-active modes, numerous Raman-inactive modes occur in the spectra, indicating distortions of the structures. Ab initio calculation of the phonon spectra on ZrB(12) excellently agrees with the experimental results. Force constants are theoretically calculated and force parameters are estimated from the Raman frequencies. The influence of the surface range on the Raman spectra is evident. The different isotopic effects (virtual crystal approximation, the polarization effect and the effect of isotopic disorder) on the phonon frequencies are determined, separated and discussed.
Glutamine Synthetase 1 Increases Autophagy Lysosomal Degradation of Mutant Huntingtin Aggregates in Neurons, Ameliorating Motility in a Drosophila Model for Huntington’s Disease (2020)

Glutamine Synthetase 1 (GS1) is a key enzyme that catalyzes the ATP-dependent synthesis of l-glutamine from l-glutamate and is also member of the Glutamate Glutamine Cycle, a complex physiological process between glia and neurons that controls glutamate homeostasis and is often found compromised in neurodegenerative diseases including Huntington's disease (HD). Here we report that the expression of GS1 in neurons ameliorates the motility defects induced by the expression of the mutant Htt, using a <i>Drosophila</i> model for HD. This phenotype is associated with the ability of GS1 to favor the autophagy that we associate with the presence of reduced Htt toxic protein aggregates in neurons expressing mutant Htt. Expression of GS1 prevents the TOR activation and phosphorylation of S6K, a mechanism that we associate with the reduced levels of essential amino acids, particularly of arginine and asparagine important for TOR activation. This study reveals a novel function for GS1 to ameliorate neuronal survival by changing amino acids' levels that induce a "starvation-like" condition responsible to induce autophagy. The identification of novel targets that inhibit TOR in neurons is of particular interest for the beneficial role that autophagy has in preserving physiological neuronal health and in the mechanisms that eliminate the formation of toxic aggregates in proteinopathies.
Notes on mammals from the Upper Cretaceous Lance Formation of Wyoming (1954)

A small collection of Cretaceous mammal teeth is described. Multituberculates are represented by 12 specimens of Meniscoessus and 18 of Cimolomys. Variants from the patterns previously described are discussed and figured. A peculiar tooth is described and compared with various dinosaurs and with the Paleocene multituberculate genus Microcosmodon. While it differs from both, it seems more like the latter. A dinosaur tooth appears to show tooth marks, indicating gnawing by a multituberculate. Marsupial teeth of four types are present. Teeth referred to Delphodon and Pediomys are unusually small for those genera, and may represent undescribed forms. Insectivore teeth are referred to Gypsonictops and Euangelistes. The latter differ in size and pattern from previously described specimens.