gebhart/patrepeval huggingface dataset:
Business Object Modeling Framework for Distributed Enterprise (1999)

In this paper the framework for business object modeling with focus on distributed enterprise is proposed. It is based on Business Object Architecture, UML and Catalysis method. Business Object Architecture is methodology bringing business semantics to component-based development – the next generation of object-oriented methodology. Basic modeling concepts are business objects, business processes and business rules. Process of business process modeling with Business Objects is described and generic modeling patterns are presented. The framework is illustrated via work effort process modeling.
DEVELOPMENT OF TEST OF SELECTED MODEL PEDESTRIAN SAFETY REGULATIONS (1981)

Two model regulations to remove parking--one from suburban streets in daylight hours and one on the last 50 feet of the approach to crosswalks--were designed in previous work to prevent pedestrian dart and dash accidents by removing screening vehicles, therby allowing pedestrians and motorists to more easily see each other and react as needed. To evaluate the safety benefits of the regulations, a study was conducted in New York City. The residential areas of Manhattan have one-side-only parking for three hours per day according to a scheme balanced by time of day and affected side of street. Accident data from 1974-1977 were screened and reports were reviewed. Based on 835 pedestrian accidents, no changes in accident distributions were found when the alternateside parking regulation was in effect. Supplementary observations in the test areas showed good but imperfect compliance with the parking bans and virtually no changes in pedestrian frequency of appearance and gross behaviors as a function of parking changes. The pattern of results plus discrepancies between the New York study situation and the model regulations meant that little could be concluded with respect to the effectiveness or noneffectiveness of the regulations. Guidelines for further research were presented, stressing the need for direct comparability to the model regulations.
Using geochemical data to identify sources of salinity to the freshwater Navajo aquifer in southeastern Utah (1995)

Ground water is an important freshwater source for domestic and livestock uses in southeastern Utah because of the arid climate and unavailability of surface water from the San Juan River. The study area includes about 1,200 square miles in the southeastern corner of Utah (fig. 1). Precipitation on mountainous areas north, south, and east of the study area (fig. 2) seeps into the Navajo and overlying aquifers where the sandstones that contain the aquifers are exposed at the surface along mountain flanks. The ground water then moves slowly away from the mountainous areas toward the area of lowest elevation 109°30' in the region, the San Juan River. The ground water reappears at land surface where it discharges as seepage to the San Juan River or is consumed by vegetation on the flood plain. Generally, wells finished in the Navajo aquifer near the San Juan River do not require pumping because water flows freely from the well casing when it is not sealed.
Measured accuracy and precision in quantitative SPECT imaging with iodine-123 (1995)

This study evaluated /sup 123/I SPECT volume and activity quantitation in terms of accuracy and precision using experimental phantom data. Two reconstruction methods were considered. The filtered backprojection (FB) method with 2D Metz pre-filtering and single iteration Chang attenuation compensation was compared with an iterative ML-EM method that used Gaussian post-filtering and incorporated the 3D divergent detector response and attenuation in the detection kernel. Both methods were examined over a range of resolution and noise characteristics. An ensemble of 21 SPECT data sets were acquired of a cylindrical phantom that contained spheres of size 21.4, 12.0, and 5.7 ml, filled with high purity /sup 123/I in a uniform background (5:1 uptake ratio). Each data set was reconstructed by both methods, and sphere volume and activity measurements were obtained from the reconstructions using a semi-automatic method that defines the region-of-interest based on the intensity gradient in the area of increased /sup 123/I concentration. The mean and standard deviation of the 21 measurements provided accuracy and precision indices, respectively. For the 12.0 ml sphere, the measured volume with either reconstruction method was accurate to within 5% of the true volume with a standard deviation of approximately 8% of the true volume. For the 12.0 ml sphere the measured volume with the iterative method exhibited substantially smaller standard deviation for equal accuracy compared with the FB method. Sphere activity was underestimated with both methods due to spatial resolution effects. The 5.7 ml sphere was generally below the size limit for volume and activity quantitation by these methods.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Parasitic absorption effects in metallic back reflectors with texture (2014)

We investigate light scattering interface textures for absorption enhancement in solar cells. If metallic films are used as back electrode or reflector, texturing mediates coupling to surface plasmon resonances which can lead to undesired absorption effects. These can be moved into uncritical spectral regions by using periodic structures with properly engineered corrugation. We use a reference structure consisting of a 1D sinusoidal grating which offers the additional advantage of resolving polarization effects of the incident light. Measured reflection data is compared with modelling results, and the suitability of different datasets in the literature is discussed.
An Investigation of Practical Approximate Nearest Neighbor Algorithms (2004)

1 Introduction The k-nearest-neighbor searching problem is to find the k nearest points in a dataset X aeRD containing n points to a query point
2,2′-(5,8-Dihydroquinoxaline-5,8-diylidene)bis(1,3-benzodithiole)s. A New Type of Electron Donors (1989)

Abstract The title compounds were prepared from quinoxaline-5,8-diones by using a Wittig-Horner reaction of 2-dimethoxyphosphinyl-1,3-benzodithiole and a retro Diels–Alder reaction. They are stronger donors than dibenzo-TTF and formed conductive complexes with TCNQ and iodine.
Effects of Methyl-.BETA.-cyclodextrin Treatment on Secretion Profile of Interferon-.BETA. and Zonula Occuludin-1 Architecture in Madin-Darby Canine Kidney Cell Monolayers (2009)

The interferon (IFN) is a paradigm of secretory protein. However, it has been poorly understood how its secretion is regulated in polarized epithelial cells. Recently, we had shown that exogenous IFNs transiently expressed in polarized monolayers were predominantly secreted to the side on which gene transfection had been performed, while stably expressed IFNs were secreted almost equally to the both cell sides. Since those modes of secretion did not affect each other, epithelial cell layers seemed to have at least two protein sorting/secretion pathways, one for transient expression and the other for stable expression, for identical secretory proteins. Furthermore, this dual secretion profile seemed to be mediated by distinct post-trans Golgi network vesicles, suggesting the involvement of lipid rafts in the sorting multiplicity. To address this issue, here we studied the effects of cholesterol depletion with methyl-beta-cyclodextrin (MbetaCD) on the secretion profile of IFN-beta exogenously expressed in Madin-Darby canine kidney (MDCK) cells. The MbetaCD-treatment, however, did not affect the profile in either transient or stable expression, although the architecture of zonula occuludin-1, which links to the tight junction, was substantially disrupted by the treatment. Further analysis of Triton X-100-insoluble cell extracts by sucrose density centrifugation demonstrated that IFN-beta was not apparently associated with lipid rafts in either transient or stable expression. These results suggest that lipid rafts may not be crucially involved in the regulation of secretion polarity of IFN-beta in the epithelial cells.
HER2 gene amplification and EGFR expression in a large cohort of surgically staged patients with nonendometrioid (type II) endometrial cancer (2008)

Type II endometrial cancers (uterine serous papillary and clear cell histologies) represent rare but highly aggressive variants of endometrial cancer (EC). HER2 and EGFR may be differentially expressed in type II EC. Here, we evaluate the clinical role of HER2 and EGFR in a large cohort of surgically staged patients with type II (nonendometrioid) EC and compare the findings with those seen in a representative cohort of type I (endometrioid) EC. In this study HER2 gene amplification was studied by fluorescence in situ hybridisation (FISH) and EGFR expression by immunohistochemistry. Tissue microarrays were constructed from 279 patients with EC (145 patients with type I and 134 patients with type II EC). All patients were completely surgically staged and long-term clinical follow up was available for 258 patients. The rate of HER2 gene amplification was significantly higher in type II EC compared with type I EC (17 vs 1%, P<0.001). HER2 gene amplification was detected in 17 and 16% of the cases with uterine serous papillary and clear cell type histology, respectively. In contrast, EGFR expression was significantly lower in type II compared with type I EC (34 vs 46%, P=0.041). EGFR expression but not HER2 gene amplification was significantly associated with poor overall survival in patients with type II EC, (EGFR, median survival 20 vs 33 months, P=0.028; HER2, median survival 18 vs 29 months, P=0.113) and EGFR expression retained prognostic independence when adjusting for histology, stage, grade, and age (EGFR, P=0.0197; HER2, P=0.7855). We conclude that assessment of HER2 gene amplification and/or EGFR expression may help to select type II EC patients who could benefit from therapeutic strategies targeting both HER2 and EGFR.
The leukotriene B4receptor functions as a novel type of coreceptor mediating entry of primary HIV-1 isolates into CD4-positive cells (1998)

The recently cloned human chemoattractant receptor-like (CMKRL)1, which is expressed in vivo in CD4-positive immune cells, has structural homology with the two chemokine receptors C-C chemokine receptor (CCR)5 and C-X-C chemokine receptor (CXCR)4, which serve as the major coreceptors necessary for fusion of the HIV-1 envelope with target cells. In view of the structural similarity, CMKRL1 was tested for its possible function as another HIV-1 coreceptor after stable expression in murine fibroblasts bearing the human CD4 receptor. The cells were infected with 10 primary clinical isolates of HIV-1, and entry was monitored by semiquantitative PCR of viral DNA. The efficiency of the entry was compared with the entry taking place in CD4-positive cells expressing either CCR5 or CXCR4. Seven of the isolates used CMKRL1 for viral entry; they were mainly of the syncytium-inducing phenotype and also used CXCR4. Entry efficiency was higher with CMKRL1 than with CXCR4 for more than half of these isolates. Three of the ten isolates did not use CMKRL1; instead, entry was mediated by both CCR5 and CXCR4. The experiments thus indicate that CMKRL1 functions as a coreceptor for the entry of HIV-1 into CD4-positive cells. In the course of this study, leukotriene B4 was shown to be the natural ligand for this receptor (now designated BLTR), which therefore represents a novel type of HIV-1 coreceptor along with the previously identified chemokine receptors. BLTR belongs to the same general chemoattractant receptor family as the chemokine receptors but is structurally more distant from them than are any of the previously described HIV-1 coreceptors.
Brainbow: New Resources and Emerging Biological Applications for Multicolor Genetic Labeling and Analysis (2015)

Brainbow is a genetic cell-labeling technique where hundreds of different hues can be generated by stochastic and combinatorial expression of a few spectrally distinct fluorescent proteins. Unique color profiles can be used as cellular identification tags for multiple applications such as tracing axons through the nervous system, following individual cells during development, or analyzing cell lineage. In recent years, Brainbow and other combinatorial expression strategies have expanded from the mouse nervous system to other model organisms and a wide variety of tissues. Particularly exciting is the application of Brainbow in lineage tracing, where this technique has been instrumental in parsing out complex cellular relationships during organogenesis. Here we review recent findings, new technical improvements, and exciting potential genetic and genomic applications for harnessing this colorful technique in anatomical, developmental, and genetic studies.
Rapid Genome Detection of Schmallenberg Virus and Bovine Viral Diarrhea Virus by Use of Isothermal Amplification Methods and High-Speed Real-Time Reverse Transcriptase PCR (2014)

Over the past few years, there has been an increasing demand for rapid and simple diagnostic tools that can be applied outside centralized laboratories by using transportable devices. In veterinary medicine, such mobile test systems would circumvent barriers associated with the transportation of samples and significantly reduce the time to diagnose important infectious animal diseases. Among a wide range of available technologies, high-speed real-time reverse transcriptase quantitative PCR (RT-qPCR) and the two isothermal amplification techniques loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) represent three promising candidates for integration into mobile pen-side tests. The aim of this study was to investigate the performance of these amplification strategies and to evaluate their suitability for field application. In order to enable a valid comparison, novel pathogen-specific assays have been developed for the detection of Schmallenberg virus and bovine viral diarrhea virus. The newly developed assays were evaluated in comparison with established standard RT-qPCR using samples from experimentally or field-infected animals. Even though all assays allowed detection of the target virus in less than 30 min, major differences were revealed concerning sensitivity, specificity, robustness, testing time, and complexity of assay design. These findings indicated that the success of an assay will depend on the integrated amplification technology. Therefore, the application-specific pros and cons of each method that were identified during this study provide very valuable insights for future development and optimization of pen-side tests.
Constraints on off-shell Higgs boson production and the Higgs boson total width in ZZ → 4ℓ and ZZ → 2ℓ2ν final states with the ATLAS detector (2018)

A measurement of off-shell Higgs boson production in the Z Z 4 and Z Z 2 2 decay channels, where stands for either an electron or a muon, is performed using data from proton-proton collisions at a centre-of-mass energy of s = 13 TeV. The data were collected by the ATLAS experiment in 2015 and 2016 at the Large Hadron Collider, and they correspond to an integrated luminosity of 36.1 fb -1 . An observed (expected) upper limit on the off-shell Higgs signal strength, defined as the event yield normalised to the Standard Model prediction, of 3.8 (3.4) is obtained at 95% confidence level (CL).
Systemically administered collagen-targeted gold nanoparticles bind to arterial injury following vascular interventions (2017)

Surgical and endovascular therapies for severe atherosclerosis often fail due to the development of neointimal hyperplasia and arterial restenosis. Our objective was to synthesize, characterize, and evaluate the targeting specificity and biocompatibility of a novel systemically injected nanoparticle. We hypothesize that surface-functionalization of gold nanoparticles (AuNPs) with a collagen-targeting peptide will be biocompatible and target specifically to vascular injury. 13 nm AuNPs were surface functionalized with a peptide-molecular fluorophore and targeted to collagen (T-AuNP) or a scrambled peptide sequence (S-AuNP). After rat carotid artery balloon injury and systemic injection of T-AuNP or S-AuNP, arteries and organs were harvested and assessed for binding specificity and biocompatibility. The T-AuNP bound with specificity to vascular injury for a minimum of 24 h. No significant inflammation was evident locally at arterial injury or systemically in major organs. The T-AuNP did not impact endothelial cell viability or induce apoptosis at the site of injury in vivo. No major changes were evident in hepatic or renal blood chemistry profiles. Herein, we synthesized a biocompatible nanoparticle that targets to vascular injury following systemic administration. These studies demonstrate proof-of-principle and serve as the foundation for further T-AuNP optimization to realize systemic, targeted delivery of therapeutics to the sites of vascular injury.
Intact Protein Quantitation Using Pseudoisobaric Dimethyl Labeling (2016)

Protein structural and functional studies rely on complete qualitative and quantitative information on protein species (proteoforms); thus, it is important to quantify differentially expressed proteins at their molecular level. Here we report our development of universal pseudoisobaric dimethyl labeling (pIDL) of amino groups at both the N-terminal and lysine residues for relative quantitation of intact proteins. Initial proof-of-principle study was conducted on standard protein myoglobin and hepatocellular proteomes (HepG2 vs LO2). The amino groups from both the N-terminal and lysine were dimethylated with HXHO (X = (13)C or C) and NaBY3CN (Y = H or D). At the standard protein level, labeling efficiency, effect of product ion size, and mass resolution on quantitation accuracy were explored; and a good linear quantitation dynamic range up to 50-fold was obtained. For the hepatocellular proteome samples, 33 proteins were quantified with RSD ≤ 10% from one-dimensional reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) analysis of the 1:1 mixed samples. The method in this study can be extended to quantitation of other intact proteome systems. The universal "one-pot" dimethyl labeling of all the amino groups in a protein without the need of preblocking of those on the lysine residues is made possible by protein identification and quantitation analysis using ProteinGoggle 2.0 with customized databases of both precursor and product ions containing heavy isotopes.
Identification of an Origin of Bidirectional DNA Replication in the Ubiquitously Expressed Mammalian CAD Gene (1995)

Most DNA replication origins in eukaryotes localize to nontranscribed regions, and there are no reports of origins within constitutively expressed genes. This observation has led to the proposal that there may be an incompatibility between origin function and location within a ubiquitously expressed gene. The biochemical and functional evidence presented here demonstrates that an origin of bidirectional replication (OBR) resides within the constitutively expressed housekeeping gene CAD, which encodes the first three reactions of de novo uridine biosynthesis (carbamoyl-phosphate synthetase, aspartate carbamoyltransferase, and dihydroorotase). The OBR was localized to a 5-kb region near the center of the Syrian hamster CAD transcriptional unit. DNA replication initiates within this region in the single-copy CAD gene in Syrian baby hamster kidney cells and in the large chromosomal amplicons that were generated after selection with N-phosphonacetyl-L-aspartate, a specific inhibitor of CAD. DNA synthesis also initiates within this OBR in autonomously replicating extrachromosomal amplicons (CAD episomes) located in an N-phosphonacetyl-L-aspartate-resistant clone (5P20) of CHOK1 cells. CAD episomes consist entirely of a multimer of Syrian hamster CAD cosmid sequences (cCAD1). These data limit the functional unit of replication initiation and timing control to the 42 kb of Syrian hamster sequences contained in cCAD1. In addition, the data indicate that the origin recognition machinery is conserved across species, since the same OBR region functions in both Syrian and Chinese hamster cells. Importantly, while cCAD1 exhibits characteristics of a complete replicon, we have not detected autonomous replication directly following transfection. Since the CAD episome was generated after excision of chromosomally integrated transfected cCAD1 sequences, we propose that prior localization within a chromosome may be necessary to "license" some biochemically defined OBRs to render them functional.
<title>Two-dimensional variation and image decomposition</title> (1998)

An image is assumed to be a blend of several statistically and semantically independent components, containing details of different information classes. Discussing about image decomposition we mean the splitting of an image onto the set of such components. Statistical distinctions of them allow to find effective algorithm for decomposition an image to several components of different properties. It gives the opportunity to extract form an image only the component of interest, thus to avoid redundant information from the following analysis, and finally to create decomposition- based image processing methods. To compare resulting images we introduce some formal quantitative measure for image estimation 2D variation, which is relied on continual image model. Its application for estimating of image decomposition is discussed. Under investigation we consider discrete model of image fragment, find rank algorithm for image decomposition, and discuss estimates of 2D variation.
Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet (2012)

The effects of 2 single exogenous and monocomponent feed enzymes, and their combination, and an ionophore coccidiostat on production performance, feed AME(n), nutrient utilization, and intestinal morphology were studied in broiler chickens. One-day-old unvaccinated and unsexed Ross 308 birds (n = 320) were kept in groups of 8 on wood shavings in pens raised from the floor and fed one of 5 experimental diets, replicated 8 times, for 36 d. Treatments were 1) a wheat-soybean meal-based feed with no added coccidiostats or exogenous enzymes (CON), 2) CON + ionophore coccidiostat (Narasin), 3) CON + xylanase (Ronozyme WX CT; XYL), 4) CON + serine protease (Ronozyme ProAct CT; PRO), or 5) CON + xylanase + serine protease (XYL+PRO). Enzymes were added on top in the feed formulation. Diets contained 0.5% TiO₂ to facilitate estimations of total tract apparent nutrient utilization. Treatments had no effect on BW gain or feed intake, but feed conversion, apparent digestibility of starch and fat, and feed AME(n) were improved with all enzyme treatments. The relative length of the ileum was reduced with XYL+PRO. For all parameters measured, the effects of XYL+PRO were similar to when XYL and PRO were fed individually. Narasin had no effect on production performance or nutrient utilization but reduced the relative lengths of jejunum and ileum. Relative lengths and weights of duodenum and cecum were unaffected by treatments. In conclusion, the improved feed conversion with both a xylanase and a protease was reflected in increased nutrient utilization, but their combination was not superior to when supplied separately. Narasin did not affect performance or nutrient utilization but reduced the relative lengths of the jejunum and ileum.