gebhart/patrepeval huggingface dataset:
The onset of PI3K‐related vascular malformations occurs during angiogenesis and is prevented by the AKT inhibitor miransertib (2022)

Low-flow vascular malformations are congenital overgrowths composed of abnormal blood vessels potentially causing pain, bleeding and obstruction of different organs. These diseases are caused by oncogenic mutations in the endothelium, which result in overactivation of the PI3K/AKT pathway. Lack of robust in vivo preclinical data has prevented the development and translation into clinical trials of specific molecular therapies for these diseases. Here, we demonstrate that the Pik3ca<sup>H1047R</sup> activating mutation in endothelial cells triggers a transcriptome rewiring that leads to enhanced cell proliferation. We describe a new reproducible preclinical in vivo model of PI3K-driven vascular malformations using the postnatal mouse retina. We show that active angiogenesis is required for the pathogenesis of vascular malformations caused by activating Pik3ca mutations. Using this model, we demonstrate that the AKT inhibitor miransertib both prevents and induces the regression of PI3K-driven vascular malformations. We confirmed the efficacy of miransertib in isolated human endothelial cells with genotypes spanning most of human low-flow vascular malformations.
Predictive Resource Management of Multiple Monitoring Applications (2010)

We propose a predictive resource management scheme for network monitoring systems that can proactively shed excess load while maintaining the accuracy of monitoring applications within bounds defined by the operator. The main novelty of our scheme is that it considers monitoring applications as black boxes, with arbitrary (and highly variable) input traffic and processing cost. This way, the monitoring system preserves a high degree of flexibility, increasing the range of applications and network scenarios where it can be used. We implemented our load-shedding-based resource management scheme in an existing network monitoring system and deployed it in a large research and educational network. We present experimental evidence of the performance and robustness of our system with multiple concurrent monitoring applications during long-lived executions and using real-world traffic traces.
Experimental Measurements of V-Ribbed Belt Tracking Lateral Forces (2000)

&lt;div class="htmlview paragraph"&gt;Two instrumented pulleys were developed to empirically measure the dynamic lateral forces of V-Ribbed belts used in automotive accessory drives. The first test pulley utilizes two cantilever beams cut into the pulley with strain gauges attached to measure the lateral dynamic forces in each individual belt rib caused by misalignment. A test stand which simulates multiple accessory drive configurations at low-end drive speeds typical in automotive engines was implemented to create the dynamic response necessary. This test stand allows variations in lateral offset, toe, camber, tension, and span length, as well as in the speed of the system through a variable speed AC motor. The second test pulley utilizes a unidirectional load cell oriented to measure the total lateral force on the test pulley. After conducting static calibration tests of the two experimental systems, dynamic results were obtained using real time data acquisition. The experimental findings were then compared with finite element analysis simulation results&lt;/div&gt;
Abstract 328: Signals Through Glycoprotein 130 Promote the Endothelial Differentiation of Cardiac Sca-1+ Cells (2007)

Background: Cardiac Sca-1+ cells were originally identified as multipotent stem cells that can be differentiated into various types of cardiac cells, including cardiomyocytes and endothelial cells....
Viral infection of cells in culture detected using infrared microscopy (2009)

FTIR microscopy has been used to collect spectra for uninfected (mock) Vero cells, and cells that have been infected with herpes simplex virus type 1 (HSV-1) and human adenovirus type 5 (Ad-5). Cells were infected at a multiplicity of infection of 10, and studied at 24 hours post exposure. The spectra for infected samples display many differences compared to the spectra for uninfected samples. To estimate how well the spectra for uninfected and infected samples could be discriminated, we used logistic and partial least squares regression methods. We show that the spectra for HSV-1 and mock infected samples are well differentiated and, for a sensitivity of 95%, we calculate a specificity of 0.999 using partial least squares regression. Spectra for Ad-5 and mock infected samples are also well differentiated. We find that applying our regression models constructed with one data set to a new validating data set still gives very high levels of specificity for a given sensitivity. Spectra for Ad-5 and HSV-1 infected samples are also differentiable. Applying our constructed regression models to new validating data, however, leads to a decrease in the discrimination capability in this instance. If one is simply interested in differentiating spectra associated with uninfected and infected cells, without distinguishing the type of infection, then we show that logistic regression models can break down whereas partial least squares regression models perform well.
Sobre a gênesis da Lei Geral de Telecomunicações e os desafios atuais (2010)

Submitted by Maria Helena Souza (mariahs@stj.jus.br) on 2014-11-17T18:06:45Z No. of bitstreams: 1 sobre_genesis_lei_herrera.pdf: 701845 bytes, checksum: 35e7523857f824e99cb429f4c1d449f0 (MD5)
Lumped element simulation of a highly integrated bioanalytical centrifugal processing unit (“BioCPU”) (2016)

The centrifugal microfluidic platforms have evolved into a mature technology for a broad range of applications, for example in the life sciences, biomedical point-of-care diagnostics, and environmental monitoring. Whilst considerable progress has been made on functional elements of the Lab-on-a-Disc (LoaD) platform, the design of complex, highly integrated networks representing sample-to-answer automation of multi-step biochemical assays remains a significant challenge. This paper addresses a developed microfluidic simulation tool for the efficient layout, simulation and analysis of high performance LoaD systems implemented by featuring a novel, “digital” flow control scheme.
Timer based burst mode protocol for high speed networks (2002)

We propose a variant of the IEEE 802.12 demand priority protocol, the timer based burst mode (TB) protocol in which an end system is allowed to transmit a burst of frames per request. The selection of the request is on the basis of the time it arrives at the hub port. A grant from the hub has a burst size associated with it. An end system is allowed to transmit as long as its burst quota is not quenched. The performance of the proposed protocol has been evaluated using simulation modeling and it has been verified that the burst mode protocol outperforms the standard demand priority protocol for Gb/s links.
Gene Delivery of Manf to Beta-Cells of the Pancreatic Islets Protects NOD Mice from Type 1 Diabetes Development (2022)

In type 1 diabetes, dysfunctional glucose regulation occurs due to the death of insulin-producing beta-cells in the pancreatic islets. Initiation of this process is caused by the inheritance of an adaptive immune system that is predisposed to responding to beta-cell antigens, most notably to insulin itself, coupled with unknown environmental insults priming the autoimmune reaction. While autoimmunity is a primary driver in beta-cell death, there is growing evidence that cellular stress participates in the loss of beta-cells. In the beta-cell fragility model, partial loss of islet mass requires compensatory upregulation of insulin production in the remaining islets, driving a cellular stress capable of triggering apoptosis in the remaining cells. The Glis3-Manf axis has been identified as being pivotal to the relative fragility or robustness of stressed islets, potentially operating in both type 1 and type 2 diabetes. Here, we have used an AAV-based gene delivery system to enhance the expression of the anti-apoptotic protein Manf in the beta-cells of NOD mice. Gene delivery substantially lowered the rate of diabetes development in treated mice. Manf-treated mice demonstrated minimal insulitis and superior preservation of insulin production. Our results demonstrating the therapeutic potential of Manf delivery to enhance beta-cell robustness and avert clinical diabetes.
Fabrication of a Multilayered Low‐Temperature Cofired Ceramic Micro‐Plasma‐Generating Device (2006)

Plasma technology is currently being used in innumerable industrial applications. Some of the common uses of this technology include surface cleaning and treatment, sputtering and etching of semiconductor devices, excitation source for chemical analyses, cutting, environmental cleanup, sterilization, and phototherapy. The harsh conditions that these devices must endure require robust refractory materials systems for their fabrication and reliability. Low‐temperature cofired ceramic (LTCC) material systems provide a durable and cost‐effective platform for the manufacture of such devices, and allow for possible integration into meso‐scale microsystems. Our designs are based on RF microstriplines that capacitively couple and ionize small gas discharge sites over the top electrode. In this paper, we have built several iterations of this micro‐plasma generating device using LTCC material systems. The impact of electrode ink selection and processing, lamination methods, dielectric layer thickness, and electrode design has been investigated. Several micro‐plasma‐generating devices were then evaluated for power requirements, output stability, and long‐term reliability.
In utero transplantation of fetal liver stem cells in humans. (1991)

Following 15 years experience in postnatal fetal liver transplantation (FLT), we have developed a new therapeutical method, namely the in utero transplantation of stem cells from the human fetal liver. This early transplant takes advantage of the immunological tolerance that exists in young fetal recipients. The three fetuses that we treated were 28, 26, and 12 weeks of age (weeks after fecundation). The first two patients had immunodeficiencies, the third one had thalassemia major. Donor cells were obtained from 7- to 10-week-old fetuses, with conditions approved by the National Committee for Bioethics. Donors and recipients were not matched. The fetal cells were infused through the umbilical vein of the first two patients and injected intraperitoneally into the third one, under ultrasonic visualization. The first patient, born in 1988, has evidence of engraftment and reconstitution of cell-mediated immunity: initially 10% than 26% of lymphocytes of donor origin (with distinct phenotype), T cell responses to tetanus toxoid and candida antigens. This child, who had bare lymphocyte syndrome, has no clinical manifestation of the disease and lives normally at home. The second child was born in 1989 and it is too early for a thorough evaluation of the immunological effects of the transplant, although donor cell engraftment has been proven (Y chromosome in this female patient). The third patient has also evidence of donor cell take (Y chromosome in a female patient) but the effect on thalassemia has not yet been fully analyzed (donor hemoglobin present in small quantity). In all three cases, no side effect of any kind developed in the mother nor in the fetus.(ABSTRACT TRUNCATED AT 250 WORDS)
Abacavir hypersensitivity reaction in primary HIV infection (2006)

Abacavir use during PHI may be associated with increased risk of hypersensitivity. As in chronic infection, HLA-B5701 is associated with the abacavir hypersensitivity reaction in PHI. Although levels of CD8 T cells and HIV RNA may be risk factors for hypersensitivity, the observed association may be due to correlation with HLA-B5701. The interesting temporal association of hypersensitivity with initiation of abacavir later in PHI merits future investigation.
Subpicosecond luminescence spectroscopy using sum frequency generation (1987)

We report an improved time-resolved luminescence spectroscopy system using sum frequency generation. The system has the following attributes: high time resolution (&amp;lt;400 fs, currently limited by the laser), ability to determine absolute zero in time delay with high precision, wide spectral range, and large dynamic range which allows measurement of luminescence under weak photoexcitation. We illustrate these with examples of time-resolved luminescence spectra from GaAs.
<title>Time domain filtering using computerized fluoroscopy--intravenous angiography applications</title> (1981)

A computerized fluoroscopy method for isolating low fluoroscopic image contrast, which results during the flow of iodinated contrast materials through cardiovascular structures is described. This technique requires applying one of a family of recursive filtering algorithms which have been designed to isolate time varying image contrast. Applications of this technique for intravenous angiography are described. Hardware implementation of these algorithms also is described and preliminary results on human subjects is presented.
Development of a Multi-Channel Telemetry System with 256 Data Channels (1982)

A new telemetry system with 256 data channels was developed to measure stresses, temperature, etc. on generator rotors during normal operation. This system was developed because it is economically impossible to measure so many points using slip rings or standard commercially available telemetry systems which normally have a finite number of data channels. In the system reported here, the rotating sensors are sampled, digitized and their signals transmitted to stationary recording instrumentation. Preliminary tests confirmed that the system can be used to centrifugal accelerations of 4,800 G. with a data accuracy of better than ±0.5%.
Photocatalytic Activity in CH3CN Related to the Surface Properties of TiO2 Powders Prepared by Sol‐Gel Method (2009)

Some TiO 2 powders, prepared from titanium(IV)tetraisopropoxide by the sol‐gel method and thermally treated between 100 and 1000 ∘ C, have been characterized by X‐ray powder diffraction and by nitrogen adsorption and desorption at 77 K to calculate the BET‐specific surface area, from which the micropore volume and the external surface area can be derived. The photocatalytic activity (k a ) of the above powders has been evaluated considering the TiO 2 ‐sensitized photo‐oxidation of 4‐methoxybenzyl alcohol in CH 3 CN as the test reaction. The decrease of k a have been related to the decrease of the BET surface area, the micropore volume, and the external surface area of the TiO 2 powders, but a satisfactory linear correlation is observed only for the last superficial parameter.
Purification of the lysosomal acid lipase from human liver and its role in lysosomal lipid hydrolysis. (1981)

The lysosomal acid lipase has been purified 2,500-fold to near homogeneity from human liver. The enzyme was converted to a soluble form by extraction of frozen tissue with Triton X-100. The enzyme, which required Triton X-100 in buffers at all purification steps for optimal yields, was stabilized by the inclusion of 33% ethylene glycol during purification. Lectin chromatography on concanavalin A-Sepharose followed by chromatography on carboxymethyl-cellulose and Sephadex G-150 provided the highly purified enzyme in 17% yield. Sodium dodecyl sulfate-acrylamide gel electrophoresis indicated that the minimum molecular weight was about 29,000 +/- 1,000. Minor protein contaminants at Mr = 58,500, 14,700 and 13,900 were present in the final preparation. A single protein band, with enzyme activity, was observed in nondenaturing acrylamide gels containing Triton X-100. Gel filtration on Sephadex G-150 in the presence of Triton X-100 gave an apparent molecular weight of about 125,000 +/- 13,000. Trioleoylglycerol, cholesterol oleate, and 1,2- and 1,3-dioleoylglycerols were substrates for the purified enzyme giving apparent Vmax values of 5,400, 1,400, 19,400, and 22,100 nmol min-1 mg of protein-1, respectively, and Km values of 0.8, 0.8, 0.9, and 1.2 mM, respectively. The recoveries of both trioleoylglycerol and cholesterol oleate hydrolytic activities were nearly identical at each purification step, suggesting that the acid lipase as single enzyme is responsible for lysosomal hydrolysis of the neutral lipids. Monooleoylglycerols were not substrates for the enzyme.
Spin Trapping with 5-Methyl-5-phenylpyrroline N-Oxide. A Replacement for 5,5-Dimethylpyrroline N-Oxide (1997)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTSpin Trapping with 5-Methyl-5-phenylpyrroline N-Oxide. A Replacement for 5,5-Dimethylpyrroline N-OxideNagaraju Sankuratri, Edward G. Janzen, Melinda S. West, and J. Lee PoyerView Author Information Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104 Cite this: J. Org. Chem. 1997, 62, 4, 1176–1178Publication Date (Web):February 21, 1997Publication History Received1 August 1996Published online21 February 1997Published inissue 1 February 1997https://pubs.acs.org/doi/10.1021/jo961476xhttps://doi.org/10.1021/jo961476xbrief-reportACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views352Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Adducts,Anatomy,Electron paramagnetic resonance spectroscopy,Hydroxyls,Quantum mechanics Get e-Alerts
Simultaneous Hydrogen Production and Electrochemical Oxidation of Organics Using Boron-Doped Diamond Electrodes (2008)

This paper presents advantages of using a boron-doped diamond (BDD) electrode for hydrogen production and wastewater treatment in a single electrochemical cell. Results indicated that the BDD electrode possessed the widest known electrochemical window, allowing new possibilities for both anodic and cathodic reactions to simultaneously take place. The BDD electrode exhibited high anodic potential, generating high oxidation state radicals that facilitated oxidation of toxic waste organic compounds such as 4-nitrophenols. In contrast, because of widening of potential windows, the rate of hydrogen evolution at the cathode was significantly increased. Time-on-stream concentrations of reaction intermediates were monitored to elucidate mechanism involved in 4-nitrophenol oxidation. Spalling, fouling, or reduction in the thickness of thin-film diamond coating was not observed. Overall, the BDD electrode exhibits unique properties including chemical inertness, anticorrosion, and extended service life. These properties are especially important in wastewater treatment. Economic advantages were attributed to the low cost and long duration BDD electrode and the valuable hydrogen byproduct produced. Analysis has shown that technology associated with the BDD electrode could be effectively implemented with minimum energy input and capital requirements. When combined with solar energy and fuel cells, electrochemical wastewater processing can become energy efficient and cost-effective.
International randomized, double-blind, placebo-controlled, phase 3 study of linsitinib (OSI-906, L) in patients (pts) with locally advanced or metastatic adrenocortical carcinoma (ACC). (2014)

4507 Background: ACC is an uncommon but frequently fatal cancer. For pts with metastatic or recurrent nonoperable ACC, there are very limited treatment options. Insulin-like growth factor 2 (IGF2) overexpression occurs in >90% of ACC, hence the IGF pathway is a potential therapeutic target. We assessed the activity of a potent IGF-1 receptor TKIlinsitinib in ACC. Methods: In a double-blind phase III trial accruing over 21 months, pts with measurable locally advanced or recurrent ACC following 1st- or 2nd-line treatment were randomly assigned to linsitinib 150mg BID orally or best supportive care & placebo (P), in a 2:1 ratio, respectively. The primary endpoint was overall survival (OS; power 80% for ñOS from 9 to 15.6 months); secondary endpoints: PFS, disease-control & objective response rate (blinded central review by RECIST 1.1), QoL & safety/toxicity (CTCAEv4.02). Results: 139 pts (median age 50yrs, 67% female, ECOG 0/1: 44.6, 51.1%) were enrolled with 90 assigned to linsitinib & 49 to P. Prior therapies included surgery: 90.6%; radiotherapy: 30.9%; mitotane: 100% & cytotoxic chemotherapy: 73.4%. The median time from diagnosis to trial initiation was 26.5 months. There was no difference between linsitinib and placebo in overall survival (median 323 days (10.8 months) vs 356d (11.8); p=0.77, HR 0.94); PFS (44 vs 46d; p=0.3, HR 0.83) & DCR (32.2 vs 34.7%). However, 3 pts on linsitinib experienced PR and 8 had prolonged PFS >100d (4 on drug >400d), whereas these events did not occur with placebo. Dose modification: L: 43.3, P 29.2%. Treatment-emerged adverse events (TEAEs) occurred in 97.8 vs 93.8%, grade 5: 5.6 vs 10.4%, gr 4: 10.1 vs 2.1%, gr 3: 45.6 vs 31.3% for L vs P respectively. Common TEAEs: fatigue (33.3 vs 22.9%), nausea (26.7 vs 31.3%), vomiting (20.0 vs 20.8%), abdominal pain (20.0 vs 20.8%), QTc prolonged (20 vs 6.3%) for L vs P. Conclusions: Targeting the IGF pathway with linsitinib did not improve overall or progression-free survival in adrenocortical cancer patients, although a small subgroup of patients seemed to benefit from this drug. Timely and efficient accrual to phase III trials in rare cancers is internationally feasible. Clinical trial information: NCT00924989.