gebhart/patrepeval huggingface dataset:
Use of n-Octyl-β-D-Thioglucoside, a New Nonionic Detergent, for Solubilization and Reconstitution of Membrane Proteins (1984)

n-Octyl-beta-D-thioglucopyranoside (octylthioglucoside), a new nonionic detergent, was synthesized. Properties of this detergent and its applicability to membrane biochemistry were investigated. The critical micelle concentration of this detergent was determined to be 9 mM. Membrane proteins of Escherichia coli were effectively solubilized with octylthioglucoside at 25-35 mM. The solubilizing power was equivalent to that of n-octyl-beta-D-glucopyranoside (octylglucoside). Reconstitution of the melibiose carrier into liposomes was performed by the detergent dilution procedure. It was found that the concentration range of octylthioglucoside for successful reconstitution (45-70 mM) was considerably wider than that of octylglucoside (43-46 mM), thus giving more reproducible results. Octylthioglucoside is more stable than octylglucoside. Furthermore, the former is synthesized with high yield at low cost whereas the latter is very expensive. Thus, we conclude that octylthioglucose is superior to octylglucoside, and is very useful in membrane biochemistry.
The Peripheral Benzodiazepine Receptor: A Promising Therapeutic Drug Target (2003)

The peripheral benzodiazepine receptor (PBR) is a critical component of the mitochondrial permeability transition pore (MPTP), a multiprotein complex located at the contact site between inner and outer mitochondrial membranes, which is intimately involved in the initiation and regulation of apoptosis. PBR is a small evolutionary conserved protein, located at the surface of the mitochondria where it is physically associated with the voltage-dependent anion channel (VDAC) and adenosine nucleotide translocase (ANT) that form the backbone of MPTP. PBR is widely distributed throughout the body and has been associated with numerous biological functions. Consistent with its localization in the MPTP, PBR is involved in the regulation of apoptosis, but also in the regulation of cell proliferation, stimulation of steroidogenesis, immunomodulation, porphyrin transport, heme biosynthesis, anion transport and regulation of mitochondrial functions. The recent literature on PBR is reviewed here. Specifically, we highlight numerous results suggesting that the use of specific PBR ligands to modulate PBR activity may have potential therapeutic applications and might be of significant clinical benefit in the management of a large spectrum of different indications including cancer, auto-immune, infectious and neurodegenerative diseases. In addition, we present the proposed mechanisms by which the molecules exerted these effects, particularly oriented on the modulation of the MPTP activities.
Phosphorus Fertility in the Strawberry Nursery (1992)

The phosphorus content is usually high in soils on which strawberry production occurs in west central Florida because of moderate P levels in the virgin soil and yearly applications of P by the growers. A P rate study was conducted to test the calibration of P for strawberry nursery production, and a randomized complete block design with four replicates was used. Rates of 0, 11, 22, and 33 kg/ha P were applied to a Seffner sand which had an initial soil P level of 86 mg/kg using the Mehlich II soil extractant. Soil tests routinely show P soil concentrations up to 250 mg/kg or greater with 86 mg/kg rated in the high range. In this study the P applied to the beds was cultivated into the soil and six plants of two strawberry clones (Fl 87-210 and Fl 85-4925) were set in each plot on 28 May 1991. All nutrients except P were applied as needed during the season. Leaf P content of daughter plants on 20 Aug 1991 varied from 0.23 to 0.25% among P treatments and were not different because of P rates. All marketable size daughter plants were harvested on 8 Oct 1991. The number, total wt, and average wt of daughter plants were not different because of applied P rates.
Solutions for Improving Spark Plug Life in High Efficiency, High Power Density, Natural Gas Engines (2006)

Short spark plug life, resulting in increased engine downtime and operating costs, is the primary factor limiting the power density and thermal efficiency in lean burn natural gas engines. Fundamentally, as engine power density increases, spark plug life decreases. Common approaches to increasing spark plug life include use of high melting temperature electrode materials and increased electrode surface area. However, future targets for engine efficiency and power density require more effective system solutions. In order to achieve these system solutions, work has been focused on developing an empirically derived electrode erosion model. This model quantifies spark plug life as a function of spark discharge characteristics, spark plug electrode design, and flow fields in the vicinity of the spark plug gap for different engine power densities. Furthermore, quenching effects resulting from large surface electrodes and smaller spark gaps have been included to verify ignitability for given in-cylinder charge density and air/fuel ratio conditions. A good agreement between experimental data and model predictions has been demonstrated. Finally, a solution for extending spark plug life in high efficiency, high power density, natural gas engines has been proposed. This solution combines high spark power with a spark plug design consisting of small electrode gap, large electrode surface, and with enhanced flow fields at the electrode gap.
Is Progesterone a Candidate Neuroprotective Factor for Treatment following Ischemic Stroke? (2009)

Gender differences in stroke outcome have implicated steroid hormones as potential neuroprotective candidates. However, no clinical trials examining hormone replacement therapy on outcome following ischemic stroke have investigated the effect of progesterone-only treatment. In this review the authors examine the experimental evidence for the neuroprotective potential of progesterone and give an insight into potential mechanisms of action following ischemic stroke. To date, 17 experimental studies have investigated the neuroprotective potential of progesterone for ischemic stroke in terms of ability to both reduce cell loss and increase functional outcome. Of these 17 published studies the majority reported a beneficial effect with three studies reporting a nil effect and only one study reporting a negative effect. However, there are important issues that the authors address in this review in terms of the methodological quality of studies in relation to the STAIR recommendations. In terms of the proposed mechanisms of progesterone neuroprotection we show that progesterone is versatile and acts at multiple targets to facilitate neuronal survival and minimize cell damage and loss. A large amount of experimental evidence indicates that progesterone is a neuroprotective candidate for ischemic stroke; however, to progress to clinical trial a number of key experimental studies remain outstanding.
Potential Transfer of Peanut and/or Soy Proteins from Poultry Feed to the Meat and/or Eggs Produced (2020)

Previous studies have demonstrated that allergenic feed proteins from peanuts in the diets of layer hens are not detected in the eggs produced. Hence, in this study, we aimed to determine if soy and/or peanut proteins in poultry feed rations of broiler chickens or layer hens would be transferred or detectable in the meat or eggs produced. To meet this objective, 99 layer hens and 300 broiler chickens were equally divided into treatment groups and fed one of three experimental diets: control soybean meal and corn diet, whole unblanched high-oleic peanut and corn diet (HO PN), or a control diet spiked supplemented with oleic acid (OA) oil. At termination, broiler chickens were processed, and chicken breast samples of the left pectoralis muscle were collected, and eggs were collected from layers. Total protein extracts from pooled egg samples and chicken breast samples were subjected to enzyme-linked immunosorbent assay (ELISA) methods and immunoblotting analysis with rabbit antipeanut agglutinin antibodies and rabbit antisoy antibodies for the detection of peanut and soy proteins. Peanut and soy proteins were undetected in all pooled egg samples and individual chicken breast meat samples using immunoblotting techniques with rabbit antipeanut agglutinin and rabbit antisoy antibodies. Moreover, quantitative ELISA allergen detection methods determined all pooled egg samples and individual meat samples as "not containing" peanut or soy allergens. Therefore, this study helps to evaluate the risk associated with the potential transfer of allergenic proteins from animal feed to the products produced for human consumption.
USP: A Lightweight File System Management Framework (2010)

With data amount growing at an ever increasing rate, file system, as the basis of data management, its functionality and content are becoming more and more complex. Thus the file system is forced to be upgraded constantly, in order to satisfy the growing demands. However, the reinvention of new file systems is always a high-cost task. Although there are frameworks such as User-space File System that may help speeding up the development process, ease the pain of deploying new functions, they also bring in significant overhead, especially when file operation frequency is fairly high. We designed and implemented a lightweight file system management framework based on LD_PRELOAD in user-space referred to as User-Space Preload (USP), and tested several management tasks with it. According to the comparison with FUSE, USP-based file system management achieved an obvious performance improvement.
Hydrate Phase Equilibria of Phosphonate Scale Inhibitors, Amines, and Ethylene Glycol (2019)

Hydrate formation is a serious concern in the production and transportation of natural gas. While there exist numerous hydrate inhibitors, there are a plethora of other chemicals that are also present alongside monoethylene glycol (MEG) for various reasons such as scale, wax, corrosion, and emulsion inhibition along the pipeline. These chemicals have not been modeled in hydrate simulators nor have their impact upon gas hydrate been studied experimentally. Thus, in this study, three scale inhibitors, iminodi(methylene) phosphonate, nitrilotris(methylene) phosphonate, and diethylenetriaminepenta(methylene) phosphonate, and two amines, monoethanolamine (MEA) and diethanolamine (DEA), were tested for methane hydrate formation using the isochoric method. The average temperature depression for each chemical as mentioned in the aforementioned order was found to be 0.06, 0.15, and 0.2 K for the scale inhibitors at a concentration of 350 ppm. This suggests that scale inhibitors may also inhibit hydrate formation, albeit at limited extent, but more importantly, they do not serve to promote hydrate formation; thus, they are not disturbing the hydrate control program. While for the amines, an average temperature depression of 0.2 and 0.47 K was found for MEA and DEA at a concentration of 5 wt %, respectively, suggesting that such amines when used alongside MEG may bring about an additional hydrate inhibitory performance.
BNT162b2 induced neutralizing and non-neutralizing antibody functions against SARSCoV-2 diminish with age (2022)

Abstract Each novel SARS-CoV-2 variant renews concerns about decreased vaccine efficacy caused by evasion of vaccine induced neutralizing antibodies. However, accumulating epidemiological data show that while vaccine prevention of infection varies, protection from severe disease and death remains high. Thus, immune responses beyond neutralization could contribute to vaccine efficacy. Polyclonal antibodies function through their Fab domains that neutralize virus directly, and Fc domains that induce non-neutralizing host responses via engagement of Fc receptors on immune cells. To understand how vaccine induced neutralizing and non-neutralizing activities synergize to promote protection, we leverage sera from 51 SARS-CoV-2 uninfected health-care workers after two doses of the BNT162b2 mRNA vaccine. We show that BNT162b2 elicits antibodies that neutralize clinical isolates of wildtype and five variants of SARS-CoV-2, including Omicron BA.2, and, critically, induce Fc effector functions. FcγRIIIa/CD16 activity is linked to neutralizing activity and associated with post-translational afucosylation and sialylation of vaccine specific antibodies. Further, neutralizing and non-neutralizing functions diminish with age, with limited polyfunctional breadth, magnitude and coordination observed in those ≥65 years old compared to &lt;65. Thus, studying Fc functions in addition to Fab mediated neutralization provides greater insight into vaccine efficacy for vulnerable populations such as the elderly against SARS-CoV-2 and novel variants.
Sub-Doppler direct infrared laser absorption spectroscopy in fast ion beams: The fluorine hyperfine structure of HF+ (1989)

We report the development of a new general technique for measuring vibration–rotation spectra of molecular ions with sub-Doppler resolution and with accurate determination of the mass and number density of the carriers of all spectral features. With this method, called direct laser absorption spectroscopy in fast ion beams (DLASFIB), we have carried out the first observation of direct absorption of photons by ions in a fast ion beam. Hyperfine-resolved vibration–rotation transitions of HF+ have been measured, and along with optical combination differences and laser magnetic resonance data, have been analyzed to yield the fluorine hyperfine parameters a, b, c and d for both v=0 and v=1 in the X 2Π state. Comparisons with many-body perturbation theory results are presented.
P60TRP interferes with the GPCR/secretase pathway to mediate neuronal survival and synaptogenesis (2010)

In the present study, we show that overexpression of the G-protein-coupled receptor (GPCR)-associated sorting protein p60TRP (transcription regulator protein) in neural stem cells (NSCs) and in a transgenic mouse model modulates the phosphorylation and proteolytic processing of amyloid precursor protein (App), N-cadherin (Cdh2), presenilin (Psen) and τ protein (Mapt). Our results suggest that p60TRP is an inhibitor of Bace1 (β-site App cleaving enzyme) and Psen. We performed several apoptosis assays [Annexin-V, TdT-mediated dUTP Nick-End Labeling (TUNEL), caspase-3/7] using NSCs and PC12 cells (overexpressing p60TRP and knockdown of p60TRP) to substantiate the neuroprotective role of p60TRP. Functional analyses, both in vitro and in vivo, revealed that p60TRP promotes neurosynaptogenesis. Characterization of the cognitive function of p60TRP transgenic mice using the radial arm water maze test demonstrated that p60TRP improved memory and learning abilities. The improved cognitive functions could be attributed to increased synaptic connections and plasticity, which was confirmed by the modulation of the γ-aminobutyric acid receptor system and the elevated expression of microtubule-associated protein 2, synaptophysin and Slc17a7 (vesicle glutamate transporter, Vglut1), as well as by the inhibition of Cdh2 cleavage. In conclusion, interference with the p60TRP/ GPCR/secretase signalling pathway might be a new therapeutic target for the treatment of Alzheimer's disease (AD).
C-Terminal Flap Endonuclease (rad27) Mutations: Lethal Interactions With a DNA Ligase I Mutation (cdc9-p) and Suppression by Proliferating Cell Nuclear Antigen (POL30) in Saccharomyces cerevisiae (2009)

During lagging-strand DNA replication in eukaryotic cells primers are removed from Okazaki fragments by the flap endonuclease and DNA ligase I joins nascent fragments. Both enzymes are brought to the replication fork by the sliding clamp proliferating cell nuclear antigen (PCNA). To understand the relationship among these three components, we have carried out a synthetic lethal screen with cdc9-p, a DNA ligase mutation with two substitutions (F43A/F44A) in its PCNA interaction domain. We recovered the flap endonuclease mutation rad27-K325* with a stop codon at residue 325. We created two additional rad27 alleles, rad27-A358* with a stop codon at residue 358 and rad27-pX8 with substitutions of all eight residues of the PCNA interaction domain. rad27-pX8 is temperature lethal and rad27-A358* grows slowly in combination with cdc9-p. Tests of mutation avoidance, DNA repair, and compatibility with DNA repair mutations showed that rad27-K325* confers severe phenotypes similar to rad27Delta, rad27-A358* confers mild phenotypes, and rad27-pX8 confers phenotypes intermediate between the other two alleles. High-copy expression of POL30 (PCNA) suppresses the canavanine mutation rate of all the rad27 alleles, including rad27Delta. These studies show the importance of the C terminus of the flap endonuclease in DNA replication and repair and, by virtue of the initial screen, show that this portion of the enzyme helps coordinate the entry of DNA ligase during Okazaki fragment maturation.
[Application of two-dimensional liquid chromatography coupled with mass spectrometry for the separation of active compounds in Gegen Qinlian decoction]. (2008)

An system of two-dimensional liquid chromatography (2D-LC) coupled with mass spectrometry was developed for the separation of components in Gegen Qinlian decoction, a complex traditional Chinese medicine. A CN column was used in the first dimensional chromatography, with gradient elution using water and methanol as the mobile phase A and mobile phase B, respectively; an ODS column was used in the second dimensional chromatography, with gradient elution using 20 mmol/L ammonium acetate buffer and acetonitrile as the mobile phase A and mobile phase B, respectively. The effluent was detected by mass spectrometry with electrospray ionization (ESI)/atmospheric pressure ionization (APCI) combined ion source and positive and negative scan modes. The components in Gegen Qinlian decoction was separated with the 2D-LC system with peak capacity up to 8000. Compared with UV detection, MS detected more components, especially in the negative mode. The 2D-LC/MS system provides higher peak capacity, better resolution and the capacity of peak identification. It is suitable for the analysis of complex samples such as the traditional Chinese medicine.
Landing Probabilities of Random Walks for Seed-Set Expansion in\n Hypergraphs (2019)

We describe the first known mean-field study of landing probabilities for\nrandom walks on hypergraphs. In particular, we examine clique-expansion and\ntensor methods and evaluate their mean-field characteristics over a class of\nrandom hypergraph models for the purpose of seed-set community expansion. We\ndescribe parameter regimes in which the two methods outperform each other and\npropose a hybrid expansion method that uses partial clique-expansion to reduce\nthe projection distortion and low-complexity tensor methods applied directly on\nthe partially expanded hypergraphs.\n
Ascertainment, classification, and impact of neoplasm detection during prolonged treatment with dual antiplatelet therapy with prasugrel vs. clopidogrel following acute coronary syndrome (2015)

ClinicalTrials.gov identifier: NCT00699998.
A Once-Through Fuel Cycle for Fast Reactors (2010)

A paradigm shift has altered the design targets for advanced nuclear energy systems that use a fast neutron spectrum. Whereas designers previously emphasized the ability of fast reactors to extend global reserves of fissile fuels, the overriding desire now is for reactor technologies that are “cleaner, more efficient, less waste-intensive, and more proliferation-resistant.” (Cheney, 2001, “U.S. National Energy Policy,” National Energy Policy Development Group, Washington, DC) This shift in priorities, along with recent design advances that enable high fuel burnup even when using fuels that have been minimally enriched, creates an opportunity to use fast reactors in an open nuclear fuel cycle. One promising route to this goal exploits a phenomenon known as a traveling wave, which can attain high burnups without reprocessing. A traveling-wave reactor (TWR) breeds and uses its own fuel in place as it operates. Recent design work has demonstrated that TWRs could be fueled almost entirely by depleted or natural uranium, thus reducing the need for initial enrichment. The calculations described here show that a gigawatt-scale electric TWR can achieve a burnup of 20%, which is four to five times that realized in current light water reactors. Burnups as high as 50% appear feasible. The factors that contribute to these high burnups and the implications for materials design are discussed.
The Reactances of Synchronous Machines (1928)

Until somewhat recently, synchronous machine theory has been satisfied with a relatively few characteristic constants, or reactances, in terms of which the behavior of machines has been calculated. Present theory, however, requires many more coefficients. There are now generally recognized two values each of leakage, synchronous, and transient reactance which correspond to the two symmetrical axes of magnetization of the armature current and which refer to balanced operation. Negative and zero phase-sequence reactances are also employed to determine operation under unbalanced conditions, and it is possible and desirable to distinguish other reactances. In view of the increasing complexity of the subject it is felt that a critical survey of it is in order and the object of the paper has been to provide that survey. The paper has been divided into two parts. Part I is descriptive, and treats the subject with regard to those factors which are important to application or operating engineers, and to designers. In particular, the major types of reactances which include the synchronous, transient, and phase-sequence reactances, are discussed. These quantities are defined and their methods of test outlined. It appears necessary to consider a second type of transient reactance, namely sub-transient reactance. Both reactances may be determined from short-circuit oscillograms as illustrated in the paper. A table is included which gives the numerical range of reactances for the various types of synchronous machines. Part II discusses the theoretical considerations, with a view to broadening and classifying existing conceptions of reactance.
Supplementary Figure 1 from The Interplay Between Neutrophils and CD8<sup>+</sup> T Cells Improves Survival in Human Colorectal Cancer (2023)

&lt;p&gt;Comparative phenotypic analysis of peripheral blood neutrophils from healthy donors and patients with CRC.&lt;/p&gt;
Muscle Studies by 1H MRS (2016)

Magnetic resonance spectroscopy (MRS) of skeletal muscle contributed significantly to our knowledge on muscle physiology in the late 1970s and 1980s, mainly by 31P-MRS in animals and human extremities. During this period, 1H-MRS was considerably less important due to limitations of the available acquisition schemes and hardware. Much-improved localization techniques and careful positioning of the selected volume reduced the vast lipid 1H signals and made investigations of small 1H resonances from other metabolites possible. It became evident that the high degree of spatial organization of skeletal muscle results in unique spectral features. Residual dipolar coupling (in particular of creatine) and susceptibility effects (resulting in a separation of intra- and extramyocellular lipids, IMCLs and EMCLs) are the major consequences of the muscular microstructure. Specific acquisition schemes allowed the observation of a series of metabolites like lactate and deoxymyoglobin, and the evaluation of diffusion, magnetization transfer, and other features of the metabolite signals in some studies with spectral and spatial resolution in multiple dimensions. The most popular applications of 1H-MRS are studies on lipid metabolism based on the link between IMCL and insulin sensitivity. In addition, measurements of various other metabolites like lactate, deoxymyoglobin, acetyl-carnitine, carnosine, and creatine are used in physiological studies.