gebhart/patrepeval huggingface dataset:
Direct Kinetic Evidence for the Formation of an Acylpyridinium Intermediate in Synthetic p-Nitrophenyl Esterase-Catalyzed Hydrolysis Reactions (1996)

The kinetics of the hydrolysis of p-nitrophenyl alkanoates 2 catalyzed by 1 were investigated in aqueous Tris buffer solution. Direct evidence for the existence of an N-acylpyridinium intermediate 3 was obtained for 1-catalyzed hydrolysis of 2 (n = 2−10). Increase of the alkyl chain length leads to an increase in the acylation rate, which reaches a maximum for 2 (n = 6). The acylation rate then decreases progressively with further increases of alkyl chain length in substrate esters. The deacylation rate was also found to exhibit a maximum for the same substrate 2 (n = 6). These results are similar to those previously reported with cholesterol esterase as catalyst for the same hydrolysis reaction. The acylation reaction is first order in catalyst concentration and exhibits saturation kinetics at high substrate concentration in accordance with the Michaelis−Menten model for enzyme reaction kinetics.
Wer profitiert von einem Workshop zur Suizidprävention an Schulen? (2022)

Zusammenfassung. Um die Suizidprävention an Schulen zu verbessern, wurden psychoedukative Workshops für Schüler_innen implementiert und angeboten. N=200 Schüler_innen der 8.–10. Jahrgangsstufe wurden zufällig einer Experimental- oder Kontrollgruppe zugewiesen. Vor und nach dem Training sowie nach drei Monaten wurden sie zu ihrem hilfesuchenden und hilfegebendem Verhalten sowie ihrer depressiven Symptomatik befragt. Die Ergebnisse weisen darauf hin, dass besonders die als stärker suizidgefährdet eingestuften Jugendlichen von dem Training durch eine Abnahme ihrer depressiven Symptomatik profitierten. Schüler_innen der Kontrollgruppe verbesserten sich demgegenüber nicht signifikant in ihrer depressiven Symptomatik. Für das hilfesuchende ebenso wie das hilfegebende Verhalten zeigten sich positive Trends, die jedoch nicht statistisch signifikant wurden. Diese Ergebnisse zeigen – wenn auch mit Limitationen aufgrund von Ausfällen durch die Coronapandemie 2020 und durch ein restriktives Vor-Screening – die Wirksamkeit einer aufwändigen außerschulischen Suizidprävention auf Befinden und Verhalten.
Characterization of a third, cII-dependent, coordinately activated promoter on phage lambda involved in lysogenic development. (1985)

Lysogenic development by bacteriophage lambda is known to require the coordinate expression of two phage operons. Coordinate control is achieved by a positive regulatory mechanism which activates transcription from the promoters of these operons, PRE and PI. The positive effector is the phage regulatory protein cII. We now identify and characterize a third cII-dependent transcription unit on phage lambda, which is positioned in the middle of the Q regulatory gene and has an anti-Q orientation. We demonstrate the cII-dependent function of this promoter and precisely map its 5' transcription start-site both in vitro and in vivo. Most importantly, we show that cII binding and transcription activation at PaQ occur at essentially the same cII levels as those required for PRE and PI activation, and that all three promoters respond to cII at the same time following phage infection. In addition, DNase protection studies demonstrate that cII selectively interacts with the same TTGCN6TTGC DNA sequence repeat in the -35 region of PaQ which cII interacts with at both PRE and PI. We find that cII also binds other TTGCN6TTGC repeat sequences on lambda but binding at these sites does not lead to productive transcription. We conclude that PaQ functions in concert with PRE and PI to regulate the lysogenic growth response of phage lambda. We presume that the PaQ directed anti-sense Q RNA transcript functions to down-regulate the expression of the Q gene, which is needed for the expression of all phage late genes during lytic growth.
POLYCHAR-10 World Forum on Polymer Applications & Theory in 2002 (2003)

A report from POLYCHAR-10 is provided. An introduction to the following papers presented at the POLYCHAR-10 Forum is given. A small selection of the authors and titles of the papers presented is listed. Prizes awarded are listed along with the composition of the Prize Committee. Some research groups of high activity which are represented at POLYCHAR every year are named.
Polarization multiplexed write-once–read-many optical data storage in bacteriorhodopsin films (2005)

In polymeric films of bacteriorhodopsin (BR) a photoconversion product, which was named the F620 state, was observed on excitation of the film with 532 nm nanosecond laser pulses. This photoproduct shows a strong nonlinear absorption. Such BR films can be used for write-once-read-many (WORM) optical data storage. We demonstrate that a photoproduct similar or even identical to that obtained with nanosecond pulses is generated on excitation with 532 nm femtosecond pulses. This photoproduct also shows strong anisotropic absorption, which facilitates polarization storage of data. The product is thermally stable and is irretrievable to the initial B state either by photochemical reaction or through a thermal pathway. The experimental results indicate that the product is formed by a two-photon absorption process. Optical WORM storage is demonstrated by use of two polarization states, but more polarization states may be used. The combination of polarization data multiplexing and extremely short recording time in the femtosecond range enables very high data volumes to be stored within a very short time.
Toxicological evaluation of a novel cooling compound: 2-(4-methylphenoxy)- N -(1 H -pyrazol-3-yl)- N -(2-thienylmethyl)acetamide (2015)

A toxicological evaluation of a novel cooling agent, 2-(4-methylphenoxy)-<i>N</i>-(1<i>H</i>-pyrazol-3-yl)-<i>N</i>-(2-thienylmethyl) acetamide (S2227; CAS 1374760-95-8), was completed for the purpose of assessing its safety for use in food and beverage applications. S2227 undergoes rapid oxidative metabolism <i>in vitro</i>, and in rat and dog pharmacokinetic studies is rapidly converted to its component carboxylic acid and secondary amine. S2227 was not found to be mutagenic or clastogenic <i>in vitro</i>, and did not induce micronuclei in polychromatic erythrocytes <i>in vivo</i>. The secondary amine hydrolysis product, <i>N</i>-(2-thienylmethyl)-1<i>H</i>-pyrazol-3-amine (M179), was also evaluated for genotoxicity. In subchronic oral toxicity studies in rats, the no-observed-adverse-effect-level (NOAEL) for S2227 was 100 mg/kg/day (highest dose tested) when administered by oral gavage for 90 consecutive days. Furthermore, S2227 demonstrated a lack of maternal toxicity, as well as adverse effects on fetal morphology at the highest dose tested, providing a NOAEL of 1000 mg/kg/day for both maternal toxicity and embryo/fetal development when administered orally during gestation to pregnant rats.
Elution and Inactivation of Bacteriophages on Soil and Cation-Exchange Resin (1979)

A variety of elution schemes was tested to determine the most effective procedure for eluting Arthrobacter bacteriophages from soil. A buffer solution of pH 8.0 was found to be the most satisfactory eluent. Bacteriophages were adsorbed to cation-exchange sites on soils, clays, and Dowex-50 resin and eluted. Eluted bacteriophages were detected by passive hemagglutination and plaque assay. Although bacteriophage antigen was successfully eluted, most recovered bacteriophages were noninfective. Inactivation was greatest in bacteriophages of Bradley group B and was associated with the elution process. Inactivation is believed to be caused by physical damage to bacteriophage tails upon elution. The significance of this inactivation relative to bacteriophage models for vertebrate viruses is discussed.
Balloon dilation angioplasty of aortic coarctations in infants and children. (1983)

Balloon dilation angioplasty (BDA) was attempted nine times in eight infants and children with aortic coarctation. In three infants (all with associated ventricular septal defect or atrioventricular canal and marked hemodynamic instability) dilation was attempted at a site of aortic narrowing that had not been operated on previously. Although the coarctation gradient fell 40% or more over the short term in two of the three, there was no angiographic or late gradient evidence of improvement. All three underwent subsequent coarctation surgery. Five dilations were performed in four infants and children who had previously undergone coarctation surgery (end-to-end anastomosis, attempted jump graft, and subclavian flap) and had residual gradients. Dilation was successful in all five cases, resulting in an increase in the diameter at the coarctation site (4.7 +/- 2.6 to 7.7 +/- 4.0 mm, p less than .05) and a decrease in the gradient measured 24 hr after dilation (42.0 +/- 15.5 to 11.8 +/- 11.2 mm Hg, p less than .05). In one child with a long area of hypoplasia of the thoracic aorta and similar lesions of the brachiocephalic vessels, a preliminary attempt to dilate a severely narrowed subclavian artery was unsuccessful. Postdilation angiography demonstrated evidence of intimal tears in three of five successful dilations. Follow-up (1 to 6 months) has demonstrated continued gradient relief in four of five children. BDA is frequently, but not always, a successful treatment for human aortic coarctation. The chief determinant of success appears to be the nature of the lesion; short-term changes in coarctation gradient are unreliable indicators of success of failure. Although BDA was not associated with mortality or significant morbidity in this group of patients, its role in the management of children with coarctation is yet to be determined.
Data-driven methodology illustrating mechanisms underlying word list recall: Applications to clinical research. (2010)

Cognitive strategy must be considered independent of overall performance before attributing poor performance to degraded learning processes. Our results demonstrate the importance of departing from global scoring techniques, especially when working with clinical populations such as patients with schizophrenia for whom episodic memory deficits are a hallmark feature.
14 GHz Operation Of Q-Switched Diode Lasers (1985)

Q-switched diode lasers have been operated in a continuous-inversion mode of Q-switching at pulse repetition rates of 14 GHz with a measurement-system-limited 40-ps FWHM pulsewidth. In continuous-inversion Q-switching, the gain in the amplifier section of the device is continuously high while the laser is modulated by an intracavity electroabsorption modulator. The low-capacitance of the modulator section of a Q-switched laser makes it attractive for high-speed modulation, especially when integrated with other electronic devices.
A 1.2 GHz CMOS dual-modulus prescaler using new dynamic D-type flip-flops (1996)

A 1.2 GHz dual-modulus prescaler IC fabricated with 0.8 /spl mu/m CMOS technology is presented in this paper. The dual-modulus prescaler includes a synchronous counter (divide-by-4/5) and an asynchronous counter (divide-by-32). A new dynamic D-flip-flop (DFF) is developed for the high-speed synchronous counter. The maximum operating frequency of 1.22 GHz with power consumption of 25.5 mW has been measured at 5 V supply voltage.
Yellow-pigmented Strains of Thermus spp. from Icelandic Hot Springs (1977)

The DNA of 13 yellow-pigmented strains of the genus Thermus, isolated from hot springs in Iceland, had a mean base composition of 60·5 to 65·1 mol % GC. In their growth characteristics and sensitivity to antibiotics, these strains resembled Thermus aquaticus strain yti. Glutamate, acetate and sucrose, with ammonium ions as a nitrogen source, supported growth. The enzymes of the tricarboxylic acid cycle were present and the glyoxylate cycle was constitutive. The stability of malate dehydrogenase in crude extracts was similar to that of strain yti.
Stereotactic Body Radiotherapy for Medically Inoperable Stage I-II Non–Small Cell Lung Cancer: The Mayo Clinic Experience (2017)

Disease control was excellent for patients who received SBRT for early-stage NSCLC, and this series represents the largest single-institution experience from the United States on SBRT for early-stage inoperable NSCLC. Higher pretreatment FDG-PET SUVmax was associated with increased risk of any recurrence, and the 50 Gy in 5 fractions dose prescription was associated with increased risk of local recurrence.
Supplementary Figure 4 from Inhibition of Akt Potentiates 2-DG–Induced Apoptosis via Downregulation of UPR in Acute Lymphoblastic Leukemia (2023)

&lt;p&gt;PDF file - 1.3MB, Dose-response of 2-DG and AIX in CCRF-CEM&lt;/p&gt;
EFAL: EDRS Feeder Link from Antarctic Latitudes - System Architecture and Operations Concept (2014)

The goal of near real-time delivery of data from spacecraft in polar low Earth orbits to the customers can be achieved by utilization of Arctic and Antarctic hubs in a Ground Station Network. Additionally, the commanding of the satellites from these hubs can provide novel opportunities for spacecraft operations. The challenge of high-rate data repatriation from such remote sites can be met by means of laser communications from ground to geostationary satellites. We present system architecture and operations concept for a setup employing the European Data Relay System (EDRS) for data uplinks from an Antarctic ground station taking into account the impact of local atmospheric conditions.
Analysis of the Integrated SEPIC-Flyback Converter as a Single-Stage Single-Switch Power-Factor-Correction LED Driver (2016)

This paper proposes a new isolated single-stage single-switch power-factor-correction (S4 PFC) driver for supplying light-emitting diodes (LEDs) without electrolytic capacitor. In the proposed LED driver, the switch turns on under zero current switching (ZCS) condition. Also, it turns on at a voltage less than its nominal voltage stress, and therefore, the switch capacitive turn-on loss decreases too much extent. The leakage energy is absorbed, so there are no voltage spikes across the switch when the switch turns off. In this paper, operating principles of the proposed driver are discussed, and design considerations are presented. Also, a laboratory prototype for supplying a 21 W/30 V LED module from 220 Vrms/50 Hz ac mains is implemented, and experimental results are presented to verify the theoretical analysis.
Reliable Low-Density DNA Array Based on Allele-Specific Probes for Detection of 118 Mutations Causing Familial Hypercholesterolemia (2005)

The proposed DNA array allows large-scale population screening and provides molecular information regarding mutation type and its correlation with clinical severity of FH, which can be used to develop therapeutic strategies.
Supplementary material to "Iodine oxoacids and their roles in sub-3 nanometer particle growth in polluted urban environments" (2023)

S1. Field measurements of sulphur dioxide (SO2) at two sites SO2 is measured continuously at the SORPES station using a Thermo TEI 43i.At BUCT/AHL station, SO2 is measured with the same analyser.Due to an instrument malfunction, SO2 concentration is discarded in October and November, 2020 at the BUCT/AHL station.The long-term time traces of daytime (08:00~16:00 LT) mean SO2 and its seasonal and monthly variations at both sites are depicted in Fig. S1.SO2 is primarily emitted through coal combustion in heating seasons in Beijing.The official onset of the heating period in Beijing is 15 th November and the heating ends on 15 th March the following year.The SO2 at BUCT/AHL site is strongly enhanced by the release of SO2 in heating seasons (grey shade area).It is worth noting that the measured concentrations of SO2 at the BUCT/AHL station is higher than that of the SORPES station in winter (January and February), which could partially explain the higher concentration of H2SO4 measured in Beijing. S2. Classification of growth time span at SORPESA Neutral cluster and Air Ion Spectrometer (NAIS, Airel Ltd., Estonia) (Manninen et al., 2016) was deployed to detect the particle number size distribution (PNSD) in the early stages of NPF at the SORPES station.The negatively charged particles in the size range of around 0.8 nm to 42 nm were measured to
ALTERNATIVE SPLICING OF PRE-mRNA: Developmental Consequences and Mechanisms of Regulation (1998)

Alternative splicing of pre-mRNAs is a powerful and versatile regulatory mechanism that can effect quantitative control of gene expression and functional diversification of proteins. It contributes to major developmental decisions and also to fine tuning of gene function. Genetic and biochemical approaches have identified cis-acting regulatory elements and trans-acting factors that control alternative splicing of specific pre-mRNAs. Both approaches are contributing to an understanding of their mode of action. Some alternative splicing decisions are controlled by specific factors whose expression is highly restricted during development, but others may be controlled by more modest variations in the levels of general factors acting cooperatively or antagonistically. Certain factors play active roles in both constitutive splicing and regulation of alternative splicing. Cooperative and antagonistic effects integrated at regulatory elements are likely to be important for specificity and for finely tuned differences in cell-type-specific alternative splicing patterns.
The Laser Beam in Urology (1968)

No AccessJournal of Urology1 Jan 1968The Laser Beam in Urology William P. Mulvaney and Carl W. Beck William P. MulvaneyWilliam P. Mulvaney and Carl W. BeckCarl W. Beck View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)62652-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1968 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byROFEIM O, HOM D, FREID R and MOLDWIN R (2018) USE OF THE NEODYMIUM: YAG LASER FOR INTERSTITIAL CYSTITIS: A PROSPECTIVE STUDYJournal of Urology, VOL. 166, NO. 1, (134-136), Online publication date: 1-Jul-2001.Razvi H, Denstedt J, Chun S and Sales J (2018) Intracorporeal Lithotripsy With the Holmium:YAG LaserJournal of Urology, VOL. 156, NO. 3, (912-914), Online publication date: 1-Sep-1996.Dretler S (2018) An Evaluation of Ureteral Laser Lithotripsy: 225 Consecutive PatientsJournal of Urology, VOL. 143, NO. 2, (267-271), Online publication date: 1-Feb-1990.Lingeman J (2018) Editorial CommentsJournal of Urology, VOL. 143, NO. 2, (271-272), Online publication date: 1-Feb-1990.Ritchey M, Patterson D, Kelalis P and Segura J (2018) A Case of Pediatric Ureteroscopic LasertripsyJournal of Urology, VOL. 139, NO. 6, (1272-1274), Online publication date: 1-Jun-1988.Watson G, Murray S, Dretler S and Parrish J (2018) The Pulsed Dye Laser for Fragmenting Urinary CalculiJournal of Urology, VOL. 138, NO. 1, (195-198), Online publication date: 1-Jul-1987.Dretler S, Watson G, Parrish J and Murray S (2018) Pulsed Dye Laser Fragmentation of Ureteral Calculi: Initial Clinical ExperienceJournal of Urology, VOL. 137, NO. 3, (386-389), Online publication date: 1-Mar-1987.Staehler G, Chaussy C, Jocham D and Schmiedt E (2018) The use of Neodymiuim-Yag Lasers in Urology: Indications, Technique and Critical AssessmentJournal of Urology, VOL. 134, NO. 6, (1155-1160), Online publication date: 1-Dec-1985.Willscher M, Filoso A, Jako G and Olsson C (2018) Development of A Carbon Dioxide Laser CystoscopeJournal of Urology, VOL. 119, NO. 2, (202-207), Online publication date: 1-Feb-1978. Volume 99Issue 1January 1968Page: 112-115 Advertisement Copyright & Permissions© 1968 by The American Urological Association Education and Research, Inc.MetricsAuthor Information William P. Mulvaney More articles by this author Carl W. Beck More articles by this author Expand All Advertisement PDF downloadLoading ...
Intrathecal phenol and glycerin in metrizamide for treatment of intractable spasms in paraplegia (1985)

Intractable lower extremity spasms after spinal cord injury is a significant source of morbidity. A case of refractory spasticity in paraplegia was successfully converted to flaccid paraplegia by intrathecal injection of phenol and glycerin in metrizamide. This chemical rhizolysis is simple and effective, and the presence of metrizamide allows both fluoroscopic guidance for accurate intrathecal phenol placement and good miscibility with cerebrospinal fluid. A brief comparative review of alternative therapeutic modalities is presented.
Bacteriology and Antibiotics Sensitivity for Pressure Sore (2007)

Purpose: Pressure sore wound develops inevitably in long-term, immobilized and hospitalized patients. Sore wound infection is common problem and makes healing process difficult. We aimed to identify the pathogens of the purulent discharge in sore wound and to obtain information for appropriate antibiotics through a sensitivity testMethods: The bacteriologic study was made on 120 cases of patients who admitted or visited our hospital from 2004 January to 2005 December for sore wound treatment. Culture material was collected in BBL transport media with cotton swab and cultured by MacConkey agar plate. The method of MIC by VITEK and Microscan was used for sensitivity test. Results: Among 120 specimens, organisms were isolated from 77(64.2%) cases. Gram positive organisms were cultured in 73 specimens, Gram negative organisms in 46 specemens, and fungi in 2 specimens. Mixed infection by Gram (+) and Gram (-) bacteria were observed in 34 specimens. Among them, S. aureus was the most common isolate in 24(31.2%) patients and 10 (13.0%) S. Aureus isolates were MRSA. The most prevalent Gram-negative organism was Escherichia coli in 20 patients(25.9%). Vancomycin and teicoplanin showed highest sensitivity to Gram-positive organisms and imipenem and amikacin to Gram-negative organisms.Conclusion: Pressure sore wound demands consideration of multimodal therapeutic aspects and these findings would be useful informations to physicians, nurses and clinical assistants in understanding the nature of sore wound and selecting appropriate antibiotics.
pycbc: Development release for O2 (2016)

Continuing to test build and bundle process.
Exact modeling of multiple access interference and BER derivation for TH-PPM UWB (2005)

A new analytical method is introduced for the exact statistical modeling of multiple access interference (MAI), in time hopping pulse position modulation (TH-PPM) ultra wideband (UWB) systems. Based on this model the exact bit error rate (BER) is expressed in simple formulas. The proposed modeling of MAI considers complete asynchronism, and is also suitable for modeling the MAI components contributed by individual paths in channels with Poisson arrivals, based on the time variables.
Foetal placental blood flow in the lamb (1972)

1. Fifteen sheep foetuses of 1.5-5.2 kg body weight were prepared with indwelling arterial and venous catheters for experimentation one to six days later.2. Unanaesthetized foetuses were found to have mean arterial and central venous blood pressures of 40 +/- 1.5 (S.E. of mean) and 2.0 +/- 0.3 (S.E. of mean) mm Hg respectively, compared to intra-uterine pressure. Intra-uterine pressure was 16 +/- 0.8 (S.E. of mean) mm Hg with respect to atmospheric pressure at mid-uterine level.3. Mean placental blood flow of the foetuses was 199 +/- 20 (S.E. of mean) ml./(min.kg body wt.). Mean cardiac output in eleven of the foetuses was 658 +/- 102 (S.E. of mean) ml./(min.kg).4. Mean foetal and maternal colloid osmotic pressures were 17.5 +/- 0.7 (S.E. of mean) and 20.5 +/- 0.6 (S.E. of mean) mm Hg respectively at 38 degrees C.5. Intravenous infusions into six ewes of 1.8 mole of mannitol and 0.4 mole of NaCl resulted in significant increases in foetal plasma osmolarity, sodium, potassium, and haemoglobin concentrations, without detectable transfer of mannitol to the foetal circulation.6. In the sheep placenta there is osmotic and hydrostatic equilibration of water. As a consequence, there should be an interaction between foetal placental blood flow and foetal water exchange with the maternal circulation. It was concluded that this interaction tends to stabilize foetal placental blood flow.