gebhart/patrepeval huggingface dataset:
Data from Diagnostic and Prognostic MicroRNAs in Stage II Colon Cancer (2023)

&lt;div&gt;Abstract&lt;p&gt;MicroRNAs (miRNA) are a class of small noncoding RNAs with important posttranscriptional regulatory functions. Recent data suggest that miRNAs are aberrantly expressed in many human cancers and that they may play significant roles in carcinogenesis. Here, we used microarrays to profile the expression of 315 human miRNAs in 10 normal mucosa samples and 49 stage II colon cancers differing with regard to microsatellite status and recurrence of disease. Several miRNAs were differentially expressed between normal tissue and tumor microsatellite subtypes, with miR-145 showing the lowest expression in cancer relative to normal tissue. Microsatellite status for the majority of cancers could be correctly predicted based on miRNA expression profiles. Furthermore, a biomarker based on miRNA expression profiles could predict recurrence of disease with an overall performance accuracy of 81%, indicating a potential role of miRNAs in determining tumor aggressiveness. The expression levels of miR-320 and miR-498, both included in the predictive biomarker, correlated with the probability of recurrence-free survival by multivariate analysis. We successfully verified the expression of selected miRNAs using real-time reverse transcription-PCR assays for mature miRNAs, whereas &lt;i&gt;in situ&lt;/i&gt; hybridization was used to detect the accumulation of miR-145 and miR-320 in normal epithelial cells and adenocarcinoma cells. Functional studies showed that miR-145 potently suppressed growth of three different colon carcinoma cell lines. In conclusion, our results suggest that perturbed expression of numerous miRNAs in colon cancer may have a functional effect on tumor cell behavior, and, furthermore, that some miRNAs with prognostic potential could be of clinical importance. [Cancer Res 2008;68(15):6416–24]&lt;/p&gt;&lt;/div&gt;
Tissue‐specific expression of sunflower heat shock proteins in response to water stress (1993)

Accumulation of mRNA and synthesis of low‐molecular‐weight heat shock proteins (LMW HSPs) was investigated in water‐stressed sunflower, under experimental conditions resulting in little or no thermal stress. Using probes and antibodies derived from developmentally expressed LMW HSPs, it was shown that homologous mRNAs and proteins accumulate in the stem and root of water‐stressed plants. This expression is quantitatively comparable with the response to heat shock: protein and mRNA accumulate to similar, high, levels and persist for comparable times during recovery from either environmental stress. However, it is shown that LMW HSPs with different molecular weights and isoelectric points are expressed in response to heat shock or water stress. Furthermore in situ localizations show a differential tissue‐specificity for the water‐stress‐ and heat‐shock‐induced LMW HSPs. Whereas the latter are localized mostly around the xylem vessels in the stem, the water‐stress‐induced proteins accumulate in the fascicular and interfascicular cambium. The possible functional implications for this specific expression are discussed.
A Study of the Complexation of Bis(m-Phenylene) Crown Ethers and Secondary Ammonium Ions (1998)

Two macrocycles, bis(m-phenylene)-26-crown-8 (1) and bis(m-phenylene)-32-crown-10 (2), were combined with several linear components, dibenzyl- (3·PF6), di-n-butyl- (4·PF6), and diphenethyl- (5·PF6) ammonium hexafluorophosphate salts. The smaller macrocycle 1 was shown not to complex effectively with any of the ammonium salts in solution by 1H NMR, but 1:1 complexes were shown to exist in the “gas phase” as [1:3], [1:4], and [1:5] by high-resolution fast atom bombardment mass spectrometry (HRFABMS) and electrospray ionization mass spectrometry (ESIMS). The X-ray crystal structure of macrocycle 1 is reported. The larger macrocycle 2 did form 1:2 complexes with the ammonium salts in solution, which were observed as well in the solid and gas phases. X-ray crystal structures of the pseudorotaxanes [2:(3)2]·2PF6 and [2:(5)2]·2PF6 were determined and are discussed. The pseudorotaxanes are stabilized by hydrogen bonding between the ammonium hydrogens of the linear salt unit and the oxygens of the crown ether macrocycle. Secondary stabilization also occurs by edge-to-face π stacking.
Large-amplitude driving of a superconducting artificial atom: Interferometry, cooling, and amplitude spectroscopy (2009)

Superconducting persistent-current qubits are quantum-coherent artificial atoms with multiple, tunable energy levels. In the presence of large-amplitude harmonic excitation, the qubit state can be driven through one or more of the constituent energy-level avoided crossings. The resulting Landau-Zener-Stueckelberg (LZS) transitions mediate a rich array of quantum-coherent phenomena. We review here three experimental works based on LZS transitions: Mach-Zehnder-type interferometry between repeated LZS transitions, microwave-induced cooling, and amplitude spectroscopy. These experiments exhibit a remarkable agreement with theory, and are extensible to other solid-state and atomic qubit modalities. We anticipate they will find application to qubit state-preparation and control methods for quantum information science and technology.
Asymmetric Assembly of Aromatic Rings To Produce Tetra‐ortho‐Substituted Axially Chiral Biaryl Phosphorus Compounds (2007)

Densely substituted title compounds can be obtained efficiently through an enantioselective [2+2+2] cycloaddition catalyzed by a cationic RhI/H8-binap complex (see scheme). This method is highly practical in view of the ready access to substrates, mild reaction conditions, operational simplicity, and high catalytic activity. cod=1,5-cyclooctadiene; H8-binap=2,2′-bis(diphenylphosphanyl)-5,5′,6,6′,7,7′,8,8′-octahydro-1,1′-binaphthyl; Ts=p-toluenesulfonyl.
Myopathy in Primary Systemic Amyloidosis (1988)

Involvement of the peripheral nervous system by amyloidosis is common. It is less well recognized that amyloid can directly infiltrate and weaken skeletal muscle. We report a case of a 73-year-old woman, known to have cardiac amyloidosis, who developed profound weakness secondary to amyloid myopathy. Review of the 8 other well documented cases in the literature has revealed a rather homogeneous syndrome. Proximal weakness, muscle stiffness, pseudohypertrophy and myalgia constitute the principal features. This syndrome usually develops in cases with well recognized generalized amyloidosis. Amyloid is deposited within the basal lamina of blood vessels and muscle fibers.
A highly acid-stable and thermostable endo-β-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus (2005)

International audience
Reconstruction of the anterior cruciate ligament using allogeneic tendon (1990)

Eighty-four patients who underwent ACL reconstruction with fresh-frozen allogeneic tendon were reviewed and evaluated with subjective and functional rating scales, physical examinations, instrumented anterior drawer tests, isokinetic testing, and arthroscopy. The average followup was 57 months (range, 36 to 90 months) and the average age at operation was 22 years (range, 16 to 37 years). The subjective and functional results were rated as excellent in 48 patients (57%), good in 31 (37%), and fair in 2 (2%). Three patients (3%) experienced a retear of the ACL. Physical examinations and instrumented anterior drawer tests showed that satisfactory anterior stability was restored in 88% of the patients. Isokinetic evaluations demonstrated that the extension torque of the involved knee recovered to a slightly lower level than that of the controls, although the flexion torque recovered to a level equivalent to that of the controls. Arthroscopic evaluations revealed that the allografts were elaborately remodeled, viable, and taut. There was no sign of immunologic rejection at any time postoperatively. Additional extraarticular procedures, consisting of both pes anserinus transfer on the medial side and iliotibial band reinforcement on the lateral side, had no effect on the results.
ChemInform Abstract: 4‐Benzoyloxy‐2‐azetidinone, a Convenient Synthon for β‐Lactam Intermediates. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Crystallinity as a Non-Destructive Indicator of Wood Hardness at Standing Trees (2015)

Wood hardness is an important property for flooring, furniture products and structural utilization. Currently wood hardness can only be measured using destructive testing. As there is no suitable method for predicting wood hardness from standing trees, developing a non-destructive technique to predict wood hardness from plantations trees would provide significant benefits for evaluating optimal silvicultural treatments, and for selecting trees for tree improvement programs. It is proposed initillay that a possible non-destructive test may be developed using „crystallinity“ characteristics (degree of crystallinity (DC), crystalite width (CW) and length (CL) and microfibril angle (MFA)) determined using X-Ray diffraction. The aim of this study was to test the feasibility of using crystallinity as a non-destructive indicator of wood hardness. Experimentally, nine trees were taken representing small, medium and large diameter from five-year old fast growing teak plantation forest in Ciampea, Bogor, Indonesia. Wood hardness and crystallinity were determined following a standard testing regime. The results showed that MFA was negatively related to all hardness directions. Crstallite width only influenced side hardness with negative correlation, while degree of crystallinity only affected the end-grain hardness with positive correlation. Different vertical positions within tree only influenced the end-grain hardness value, while different radial positions had no effect on wood hardness. The optimum sampling height was found to be 80 cm, 130 cm, and 100 cm for radial, tangential and end-grain hardness, respectively. The relationship was based on the height that provided the highest correlation with the whole tree hardness values. Proposed models involving density and „crystallinity“ for predicting wood hardness are presented. While the R 2 from the proposed models was mostly less than 0.50, however, the approach described provides a new way to predict wood hardness from 10 mm increment cores at standing trees.
Designing the architecture of a preliminary system for assisting tactile exploration of bas-reliefs (2017)

The participation of blind people (BP) in cultural life can be improved by enhancing methodologies and tools for facilitating the 3D reproduction of artworks and, at the same time, to facilitate their exploration. The creation of integrated systems including not only tactile media such as, for instance, sculptures or bas-reliefs but even a set of tools capable of providing the users with an experience cognitively comparable to the one originally envisioned by the artist, may undoubtedly enhance artworks exploration. The present work aims to design a conceptual system to aid BP in the tactile exploration of bas-reliefs. This conceptual system comprises both a hand-tracking system and an audio device, together with a number of methodologies, algorithms and information related to physical design. The designed layout, preliminarily tested to understand its potential and limits, proved to be a promising first attempt in enhancing the BP exploration experience.
Continuous Laboratory Gold Solvent Extraction from Cyanide Solutions using LIX 79 Reagent (2007)

Abstract Continuous laboratory solvent extraction of gold from cyanide solutions has been investigated by using LIX 79 guanidine‐based extractant. Different variables that affected the extraction included aqueous pH, extractant concentration and modifier concentration. Extraction isotherms of the aurocyanide complex with respect to the other cyanoanions were compared, and the following order of selectivity was observed: Au &gt; Ag &gt; Cu &gt; Zn &gt; Fe. According to the pH isotherms, aurocyanide can be extracted in alkaline media, and a better separation with respect to other cyano anions was obtained in the pH range 10.5–11.2. From the McCabe‐Thiele diagrams, better recovery was observed when using LIX 79 and tridecanol at 10 vol.‐%. Stripping gold from the loaded organic was carried out at pH &gt; 12 by using NaOH and NaCN solutions. The pilot plant tests indicate that a two‐stage extraction followed by one strip step are more than adequate to obtain an overall process efficiency of 92 %. However, for those cases where copper is present significantly, a copper wash stage is recommended before gold stripping. In this case, stripping of copper is accomplished at a pH 10.8, whereas the gold stripping was done at a pH of 12.0.
Diatom Biosilica in Targeted Drug Delivery and Biosensing Applications: Recent Studies (2022)

Diatoms are single-cell algae encased in a cell wall (named frustule) composed of transparent, biogenic (or opaline) silica with intricate and strikingly regular patterns. Over the past 30 years, these microorganisms have proven to be a valuable replacement for synthetic silica that satisfies numerous pharmaceutical requirements for the realization of drug delivery vectors, biosensing supports and photonic crystals. It is not only the structural features of the diatoms, but also the possibility of chemically modifying the frustule that permits the relatively straightforward transformation of the biosilica into potential devices for biomedical applications. In this short review, we explore the applications of diatoms-derived biosilica in the drug delivery and biosensing fields. Specifically, we consider the use of diatoms for the targeted delivery of anticancer and antibiotic drugs and how the same microalgae are employed in the fabrications of biosensors whose analyte signal response is evaluated via fluorescence and surface-enhanced Raman scattering techniques. We limit our discussion to studies published in the last seven years, with the intention of minimizing possible redundancy with respect to previously published contributions.
A new approach to the preparation of IR transparent Ca12Al14O33 material (1993)

Calcium aluminate Ca12Al14O33 glass, on crystallisation around 1150-degrees-C, shows IR transparency beyond 6 mum which is unusual in ceramic materials and renders it suitable for use in IR optical devices. The material retains its IR transparency even on hydration. (C) 1993 The Institute of Materials.
A Dual-band Rectenna with Improved RF-DC Sensitivity for Wireless Energy Harvesting (2019)

A rectenna with improved output DC voltage for dedicated wireless energy harvesting at both of 2.4 GHz and 5.8 GHz bands is presented. A wideband Vivaldi antenna with coupled feed along with a dual band 2-stage charge pump was designed for dual-band harvesting in the 2.4 and 5.8 GHz bands. Measurements show a DC output voltage of over 1.8V with only 2.14 μW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> at 2.4 GHz and 18μW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> at 5.8GHz of incident wireless power.
Experimental recombination rate coefficients for O2 (2004)

The electron-ion recombination and electron-impact ionization of several astrophysically abundant C, N, O, and Ni ions have been investigated using the synchrotron storage ring CRYRING. The recombination rate coefficients predominanty used in the analyses of emission line spectra and for ionization balance calculations are determined from isoelectronic fits and extrapolations of theoretical and limited experimental results available from the literature. The rate coefficients reported by various authors differ by an order of magnitude for various ion species. For low temperature plasmas(<104 K), the available information on resonant recombination rate coefficients is even more limitied and, unfortunately, most available data has been determined from LS coupling calculations, which can have adverse effects on recombination rates due to the mistreatment of autoionizing states. For ions of C, N, and O, the production of metastable states complicates the experimental rate coefficient determinations. Some attempts have been made in this work to utilize both recombination and electron-impact ionization in estimating the metastable ion beam fractions present. Other metastable fractions have been determined by comparing the experimental recombination spectra to intermediate coupling calculations. Some of the absolute recombination rate coefficients reported in this work are given for the first time while many others vastly improve the spectral resolution of previous experiments, allowing for a more detailed study of the low energy resonances which contribute to the resonant recombination rate coefficient at low plasma electron temperatures. This increased resolution has also lead to the observation of trielectronic recombination resonances which greatly influence low temperature recombinaion rates in some ions.
Light-Induced Evolution of Silicon Quantum Dot Surface Chemistry—Implications for Photoluminescence, Sensing, and Reactivity (2014)

The photoluminescence of freestanding silicon quantum dots (QDs) responds rapidly to various gases in the atmosphere. Under short wavelength irradiation, the luminescence can change over a time frame of seconds to minutes and can feature orders-of-magnitude shifts in the luminescence intensity and rapid “jumps” in the peak wavelength. Starting with hydride-terminated Si QDs, the luminescence can be reversibly quenched or strongly activated, leading to a partly reversible, three-state luminescence condition consisting of regular, quenched, and activated states. These changes were characterized using photoluminescence spectroscopy, Fourier transform infrared spectroscopy, and electron spin resonance. The response is a multistep process involving several stages, including energy transfer, abstraction of hydrogen, and rapid optical-irradiation-assisted oxidation. These properties make freestanding Si QDs a potentially attractive material for optical sensor applications.
Adult Pituitary Cell Maintenance: Lineage-Specific Contribution of Self-Duplication (2013)

The identification of a stable pool of progenitor/stem cells in the adult pituitary has renewed the interest of identifying mechanisms for maintenance of pituitary cells throughout life. Whereas developmental studies have shown that progenitor expansion is the major source of new differentiated cells during pituitary organogenesis, the contribution of these progenitors for maintenance of the adult tissue is not clear although progenitors were clearly involved in cell expansion following end-organ ablation, notably after adrenalectomy and/or gonadectomy. We have used a genetic trick that eliminates dividing cells by apoptosis in order to assess the contribution of differentiated corticotropes and melanotropes for maintenance of their population in the adult pituitary. The system relies on chromosome instability created by the action of the Cre recombinase on inverted loxP sites. Expression of Cre recombinase in corticotropes and melanotropes led to progressive loss of corticotropes whereas melanotropes were unaffected. Because the Cre transgene is not expressed in progenitors, the data indicate that maintenance of the adult corticotrope pool is primarily due to self-duplication of differentiated cells. In contrast, melanotropes do not divide. Maintenance of corticotropes by self-duplication contrasts with the reported proliferative response of undifferentiated cells observed after adrenalectomy. If corticotrope reentry into cell cycle constitutes a normal mechanism to maintain the adult corticotrope pool, this same mechanism may also be perturbed during corticotrope adenoma development in Cushing's disease.
A conjugate-gradient sparse-matrix method for efficient computation of a cardiovascular model (2007)

A conjugate-gradient (CG) sparse-matrix (SIM) method was applied to an integrated cardiovascular model for improving its computational efficiency. The model was previously developed by combining a 3D finite element model (FEM) of the left ventricle with an analog electrical model of the circulatory system. The computation of this integrated model was dominated by the FEM, which consumed 99.9% of the run time. A computational bottleneck (98.6%) was the solution of the simultaneous equations in the global stiffness matrix and the nodal displacement vectors. For a 40% infarct size in the FEM the computational time for a cardiac cycle with a 5-ms time resolution and a heart rate of 71 bpm was reduced from 20 min to 6 min. Depending on the infarct size, the CGSM method reduced the computational time to 30-60% of that obtained by use of a conventional QR decomposition method.
Unexplored Nucleotide Binding Modes for the ABC Exporter MsbA (2018)

The ATP-binding cassette (ABC) transporter MsbA is an ATP-driven lipid-A flippase. It belongs to the ABC protein superfamily whose members are characterized by conserved motifs in their nucleotide binding domains (NBDs), which are responsible for ATP hydrolysis. Recently, it was found that MsbA could catalyze a reverse adenylate kinase (rAK)-like reaction in addition to ATP hydrolysis. Both reactions are connected and mediated by the same conserved NBD domains. Here, the structural foundations underlying the nucleotide binding to MsbA were therefore explored using a concerted approach based on conventional- and DNP-enhanced solid-state NMR, pulsed-EPR, and MD simulations. MsbA reconstituted into lipid bilayers was trapped in various catalytic states corresponding to intermediates of the coupled ATPase-rAK mechanism. The analysis of nucleotide-binding dependent chemical shift changes, and the detection of through-space contacts between bound nucleotides and MsbA within these states provides evidence for an additional nucleotide-binding site in close proximity to the Q-loop and the His-Switch. By replacing Mg<sup>2+</sup> with Mn<sup>2+</sup> and employing pulsed EPR spectroscopy, evidence is provided that this newly found nucleotide binding site does not interfere with the coordination of the required metal ion. Molecular dynamic (MD) simulations of nucleotide and metal binding required for the coupled ATPase-rAK mechanism have been used to corroborate these experimental findings and provide additional insight into nucleotide location, orientation, and possible binding modes.
Risk Factors for Postoperative Complications of Percutaneous Nephrolithotomy at a Tertiary Referral Center (2015)

At our center percutaneous nephrolithotomy is an excellent option for complex kidney stone management with a low overall complication rate. Older patient age and upper pole access are significantly associated with an increased risk of a major complication.
Effects of Plasma-Polymerized Coatings on the Frictional Behavior of Beryllium Surfaces (1992)

Eight different polymers were coated on beryllium surfaces by glow discharge polymerization in an inductively coupled tubular reactor. Using a pin-on-disk apparatus, friction tests were then conducted on the polymer-coated beryllium disks with Pyroceram pins. Of all the coated surfaces, polyethylene-coated beryllium exhibited the lowest friction with no measurable wear. The friction coefficient of the polyethylene-coated beryllium surface was found to depend strongly on the film thickness. The variation of friction coefficient with film thickness was analyzed, and a reasonable agreement between theoretical prediction and experimental data was obtained.