gebhart/patrepeval huggingface dataset:
Zhidai Decoction Inhibits Cervical Cancer through Regulation of Vaginal Microbiota (2020)

Cervical cancer is one of the top lethal malignancies among women worldwide. The current treatment methods have so many drawbacks that new treatment methods need to be developed. Zhidai Decoction (ZDD) is an effective traditional Chinese herbal formulation for gynecological diseases. Its main effect is controlling abnormal leucorrhea which is a typical early clinical manifestation of cervical cancer. However, how ZDD directly affects cervical cancer has not been addressed. In this study, we established a mouse cervical cancer U14 cell subcutaneous transplantation tumor model and took an early intervention with ZDD to evaluate the antitumor effect of ZDD. In addition, we also investigated the regulatory effects of ZDD on the vaginal microbiota using 16S rRNA analysis in this study. Our results showed that ZDD can significantly improve systemic symptoms and reduce vaginal secretions of tumor-bearing mice. Compared with the CCM group (the cervical cancer model group), in the ZDD-treated group, the tumor inhibitory rate was 37.90%, the average daily food intake of mice was increased to 5.27 ± 0.74 g (<i>P</i> < 0.05), and the survival time was obviously prolonged to 21 days (<i>P</i> < 0.05). Analysis of the sequencing results of 16S rRNA showed that the main microbial genera of the CCM group were <i>Pasteurella</i> (27.20%) and <i>Helicobacter</i> (18.50%), while those in the ZDD group were <i>Staphylococcus</i> (13.22%) and <i>Lactobacillus</i> (4.68%). It revealed that ZDD has the effect of regulating the vaginal microbiota of cervical cancer, especially in increasing the relative abundance of <i>Lactobacillus</i> and <i>Staphylococcus</i> and decreasing the relative abundance of <i>Pasteurella</i> and <i>Helicobacter</i>. The analysis also showed that ZDD could adjust microbiota structure, species abundance, and community compositions of vaginal microbiota. In conclusion, ZDD displayed inhibitory effect on cervical cancer, and it might be based on restoring the balance of vaginal microbiota. Furthermore, our conclusion supports the promotion of ZDD in the early treatment of cervical cancer.
Unified Parkinson's disease rating scale characteristics and structure (1994)

Our purpose was to verify some basic aspects of validation of the Unified Parkinson's Disease Rating Scale (UPDRS). One hundred and sixty-seven Parkinson's disease (PD) patients were included. Group A (n = 40) was simultaneously assessed by five raters who applied the UPDRS and other PD rating scales (PDRS). A set of timed tests, the Mini-Mental State Examination (MMSE), and the Hamilton Scale for Depression (HSD) were administered by an independent examiner. Group B (n = 127) was individually assessed through the UPDRS and the other PDRSs by one neurologist in four different hospitals. The UPDRS was administered in 16.95 +/- 7.98 min. The internal consistency was high (Cronbach's alpha = 0.96). Nevertheless, the items related to depression, motivation/initiative, and tremor were scarcely consistent. The Interrater reliability was satisfactory (all the items had k > 0.40). There was a high correlation of the UPDRS with the Hoehn and Yahr staging (rs = 0.71; p < 0.001) and some timed tests (finger tapping; arising from chair), but also with the MMSE and HSD (rs = 0.53; rs = 0.64; p < 0.001). The convergent validity with the other PDRS (Intermediate Scale and Schwab and England Scale) was very high (rs = 0.76-0.96; p < 0.001). The factor analysis identified six factors that explained 59.6% of the variance. The dimension "tremor" showed a remarkable independence. The UPDRS is a multidimensional, reliable, and valid scale, with some inconveniences derived from its internal consistency, discriminant validity, and pragmatic application.
Hepatic transplantation survival: Correlation with adenine nucleotide level in donor liver (1988)

The hypothesis was tested that human donor livers with higher ATP content and energy charge achieve better results after hepatic transplantation. Biopsies were obtained from 25 donor livers immediately prior to implantation and analyzed for adenine nucleotides using high-performance liquid chromatography. The results were correlated with organ histology, transplant function and outcome. Significantly higher concentrations of ATP (4.22 +/- 2.87 vs. 0.71 +/- 0.69 nmoles per mg protein, p less than 0.01), ADP (8.75 +/- 2.96 vs. 4.49 +/- 1.95 nmoles per mg protein, p less than 0.01) and energy charge (0.43 +/- 0.15 vs. 0.21 +/- 0.04, p less than 0.02) were found in successful (n = 20) relative to failed (n = 5) livers. No significant differences were found in AMP, xanthine or hypoxanthine for the two groups, although the average values were higher in failed livers. Fifteen recipients with liver ATP concentration above 2 nmoles per mg protein and energy charge above 0.3 recovered well. Five other successful patients with lower ATP concentration (0.70 +/- 0.39 nmoles per mg protein) and energy charge (0.20 +/- 0.03) had postoperative courses complicated by infection or prolonged hyperbilirubinemia. In five patients whose livers failed, all had low ATP content and energy charge. Of these, three received a replacement liver and two died shortly after the transplantation. The study demonstrates a direct correlation between high ATP content and good posttransplant outcome.
Topical imiquimod as treatment for different kinds of cutaneous lymphoma. (2007)

Imiquimod as a topical immune response modifier leads to a localized production of interferon and other cytokines. Apart from its use for genital warts it has therefore been used as treatment for different cutaneous neoplasms, including a few cases of cutaneous T-cell lymphoma. We treated 8 patients (4 with mycosis fungoides, 1 with CD30+ anaplastic large cell lymphoma and 3 with primary cutaneous B-cell lymphoma) with topical imiquimod. Therapy was started three times per week, in cases without response, the frequency was increased to a daily application. Two patients with mycosis fungoides and the patient with the CD30+ anaplastic large cell lymphoma had a complete clinical remission, the other two patients with mycosis fungoides did not show a response to imiquimod. Of the patients with cutaneous B-cell lymphoma, two reached a partial remission, one did not respond to therapy. Two patients had side effects such as erythema and pruritus which disappeared when the frequency of therapy was reduced. Our preliminary data show that imiquimod might be effective in some cases with therapy resistant lesions of cutaneous T-cell lymphoma as well as of cutaneous B-cell lymphoma, but more controlled studies are needed.
The Multi‐functional Enzyme CHIP Regulates Microtubule Dynamics (2020)

The C arboxy‐terminus of H sc70 I nteracting P rotein (CHIP) controls several aspects of protein quality control machinery through its ability to function as a co‐chaperone, autonomous chaperone, and ubiquitin ligase. CHIP is ubiquitously expressed in all tissues, but interestingly, CHIP expression is higher in certain tissues such as heart, muscle and brain, suggesting an increased dependence on CHIP function in these organ systems. This concept is supported by studies that show how altered CHIP function associates with several ailments such as cardiac diseases, neurological disorders, and cancer. Microtubules (MTs) are a part of the cellular cytoskeleton that provides shape and structure to cells. In addition to these roles, MTs play a crucial role in cell division, migration, intracellular organization and trafficking. Dynamic assembly and disassembly of MTs upon various stimuli is important for the proper functioning of differentiated cells and is a process orchestrated by multiple proteins. Dysregulation in the MT reorganization machinery is implicated in a similar spectrum of diseases associated with the loss of CHIP function, however, there has yet to be a direct connection between CHIP and MT dynamics. We performed a proteomics screen on wild type and CHIP −/− mouse embryonic fibroblasts and identified differential regulation of proteins that regulate several aspects of MT function, such as STMN1 and PDLIM. Given the importance of CHIP in protein homeostasis, we hypothesized that CHIP is necessary for proper MT dynamics in response to cellular stimulations. We found that CHIP is required for the phosphorylation of stathmin, a regulator of MT assembly, and for the tubulin polymerization in C2C12 myoblast cells following insulin and glucose stimulation. This stimulation triggers an important MT‐dependent process to mediate glucose uptake. Furthermore, ex vivo studies using muscle fibers isolated from CHIP −/− mice failed to form MT lattices after glucose stimulation, consistent with a new role for CHIP in regulating MT in muscle. Altered MT dynamics, as well as CHIP loss of function, are also implicated in neurodegenerative diseases, particularly those associated with proteinopathies. To determine if CHIP contributes to MT regulation in neurons, we used an immunoprecipitation‐mass spectrometry approach and identified changes in interactions between CHIP and MT proteins during proteotoxic stress. Future experiments will focus on validating the interaction data and further dissecting the specific functions of CHIP that contribute to the regulation of MT dynamics using biochemical approaches and imaging. In conclusion, for the first‐time, our data couple CHIP to MT dynamics, and suggest that CHIP may contribute to cell protection by regulating the proteins that are vital to MT dynamics. Support or Funding Information Funding was provided by a grant to J.C.S. from the National Institute of Aging (R01‐GM061728).
Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar gold arrays (2016)

The detection of biomarkers by means of Surface Enhanced Raman Spectroscopy (SERS) is foreseen to became a very important tool in the clinical practice because of its excellent sensitivity and potential for the simultaneous detection of multiple biomarkers. In the present paper we describe how it was possible to build a sensor for the detection of genetic biomarkers involved in acute myeloid leukemia. The assay is based on the use of a specifically designed SERS substrate made of a 2D crystal structure of polymeric pillars embedded in a gold layer. This substrate is characterized by good enhancing properties coupled with an excellent homogeneity. The SERS substrate was conjugated with DNA probes complementary to a target sequence and used in a sandwich assay with gold nanoparticles labeled with a second DNA probe and a Raman reporter. The so developed assay allowed the detection of a leukemia biomarker (WT1 gene) and an housekeeping gene with low picomolar sensitivity. At last, we optimized the assay in order to tackle one of the main limitations of SERS based assay: the loss of signal that is observed when the Raman spectra are collected in liquid. Combining a preferential functionalization on the polymeric pillars with a different height of the polymer pillars from the gold layer the assay demonstrated its effectiveness even when measured in buffer.
Graphene-decorated graphite–sulfur composite as a high-tap-density electrode for Li–S batteries (2015)

FESEM images of graphite, graphene and sulfur individually and their composite with high-tap-density with improved volumetric energy density over 50 cycles to that of graphene/sulfur composite (low tap-density).
Expression and Function of Tetraspanins and Their Interacting Partners in B Cells (2018)

Tetraspanins are transmembrane proteins that modulate multiple diverse biological processes, including signal transduction, cell-cell communication, immunoregulation, tumorigenesis, cell adhesion, migration, and growth and differentiation. Here, we provide a systematic review of the involvement of tetraspanins and their partners in the regulation and function of B cells, including mechanisms associated with antigen presentation, antibody production, cytokine secretion, co-stimulator expression, and immunosuppression. Finally, we direct our focus to the signaling mechanisms, evolutionary conservation aspects, expression, and potential therapeutic strategies that could be based on tetraspanins and their interacting partners.
Peer-Assisted VoD Systems: An Efficient Modeling Framework (2014)

We analyze a peer-assisted Video-on-Demand (VoD) system in which users contribute their upload bandwidth to the redistribution of a video that they are downloading or that they have cached locally. Our target is to characterize the additional bandwidth that servers must supply to immediately satisfy all requests to watch a given video. We develop an approximate fluid model to compute the required server bandwidth in the sequential delivery case, as well as in controlled nonsequential swarms. Our approach is able to capture several stochastic effects related to peer churn, upload bandwidth heterogeneity, and nonstationary traffic conditions, which have not been documented or analyzed before. Finally, we provide important hints for the design of efficient peer-assisted VoD systems under server capacity constraints.
Computational Optimization of Adaptive Hybrid Darrieus Turbine: Part 1 (2019)

Darrieus-type Vertical Axis Wind Turbines (VAWT) are promising for small scale decentralized power generation because of their unique advantages such as simple design, insensitive to wind direction, reliability, and ease of maintenance. Despite these positive aspects, poor self-starting capability and low efficiency in weak and unsteady winds deteriorate further development. Adaptive Hybrid Darrieus Turbine (AHDT) was proposed by the author in the past study as a potential solution to enhance low wind speed characteristics. The objective of the current research is to optimize the parameters of AHDT. AHDT integrates a dynamically varying Savonius rotor with a Darrieus rotor. A fully detailed 2D numerical study employing Reynold-Averaged Navier Stokes (RANS) is carried out to investigate the impact of the Darrieus rotor diameter (DR) on the Savonius rotor (DT) with regard to hybrid turbine performance. The power coefficient of the Darrieus rotor is evaluated when the Savonius rotor is in the closed condition (cylinder) of various diameters. The influence of Reynolds number (Re) on the torque coefficient is examined. Power loss of 58.3% and 25% is reported for DR/DT ratio of 1.5 and 2 respectively for AHDT with solidity 0.5 at 9 m/s. The flow interaction between the Savonius rotor in closed configuration reveals the formation of von Karman vortices that interact with Darrieus blades resulting in flow detachment. An optimum diametrical ratio (DR/DT) of 3 is found to yield the maximum power coefficient of the Darrieus rotor.
Life and death as a T lymphocyte: from immune protection to HIV pathogenesis (2009)

Detailed analysis of T cell dynamics in humans is challenging and mouse models can be important tools for characterizing T cell dynamic processes. In a paper just published in Journal of Biology, Marques et al. suggest that a mouse model with its activated CD4(+) T cells are deleted has relevance for HIV infection.
Mercury CSD 2.0– new features for the visualization and investigation of crystal structures (2008)

The program Mercury , developed by the Cambridge Crystallographic Data Centre, is designed primarily as a crystal structure visualization tool. A new module of functionality has been produced, called the Materials Module , which allows highly customizable searching of structural databases for intermolecular interaction motifs and packing patterns. This new module also includes the ability to perform packing similarity calculations between structures containing the same compound. In addition to the Materials Module , a range of further enhancements to Mercury has been added in this latest release, including void visualization and links to ConQuest , Mogul and IsoStar .
Towards Defect Detection in Metal SLM Parts Using Modal Analysis "Fingerprinting" (2017)

The validation of Additively Manufactured (AM) materials is a difficult and expensive process because the local engineering properties are a function of the thermal history. The thermal history varies with the process parameters, as well as the part geometry. This paper presents a case study using modal testing to identify defects in realistic AM parts. A setup consisting of a Scanning Laser Doppler Vibrometer (LDV) was used to identify the resonant frequencies for several geometrically identical parts on a build plate. Parts with suboptimal process parameters from purposely varying the process parameters, are identified by a shift in the mode peak frequency. Results from this study are compared to Finite Element Analysis (FEM) models and generalized for identifying defects in parts created with AM on the basis vibration/modal “fingerprinting.”
THE FIRST FERMI LAT SUPERNOVA REMNANT CATALOG (2016)

ABSTRACT To uniformly determine the properties of supernova remnants (SNRs) at high energies, we have developed the first systematic survey at energies from 1 to 100 GeV using data from the Fermi Large Area Telescope (LAT). Based on the spatial overlap of sources detected at GeV energies with SNRs known from radio surveys, we classify 30 sources as likely GeV SNRs. We also report 14 marginal associations and 245 flux upper limits. A mock catalog in which the positions of known remnants are scrambled in Galactic longitude allows us to determine an upper limit of 22% on the number of GeV candidates falsely identified as SNRs. We have also developed a method to estimate spectral and spatial systematic errors arising from the diffuse interstellar emission model, a key component of all Galactic Fermi LAT analyses. By studying remnants uniformly in aggregate, we measure the GeV properties common to these objects and provide a crucial context for the detailed modeling of individual SNRs. Combining our GeV results with multiwavelength (MW) data, including radio, X-ray, and TeV, we demonstrate the need for improvements to previously sufficient, simple models describing the GeV and radio emission from these objects. We model the GeV and MW emission from SNRs in aggregate to constrain their maximal contribution to observed Galactic cosmic rays.
La Evaluación del Desempeño por Competencias: Percepciones de Docentes y Estudiantes en la Educación Superior (2016)

ResumenEl objetivo de la investigación fue conocer las percepciones de estudiantes y docentes sobre el instrumento para evaluar el desempeño docente en universidades públicas angolanas y el orden de prioridad que le confieren a cada dimensión.El estudio se realizó en la Universidad José Eduardo dos Santos, institución representativa del universo a investigar.El instrumento de evaluación está estructurado en 23 afirmaciones distribuidas en cuatro dimensiones: competencias pedagógicas-didácticas, investigativas, de gestión académica y de extensión universitaria.Este instrumento se aplicó a una muestra conformada por 126 docentes y 342 estudiantes.El análisis de las percepciones de estudiantes y profesores permitió concluir sobre la efectividad de utilizar las cuatro dimensiones propuestas para evaluar el desempeño docente.De acuerdo con las opiniones de ambos grupos la dimensión de las competencias pedagógicas-didácticas ocupa la primera posición.
P-selectin mediates metastatic progression through binding to sulfatides on tumor cells (2006)

Hematogenous carcinoma metastasis is associated with tumor cell emboli formation, which is now known to be facilitated by selectins. P-selectin-mediated interactions of platelets with cancer cells are based mostly on mucin- and glycosaminoglycan-type selectin ligands. We previously showed that mouse colon carcinoma cells (MC-38) carry P-selectin ligands of nonmucin origin, which were not identified. Here we show that P-selectin ligands recognized on MC-38 cells are sulfated glycolipids, thereby facilitating experimental metastasis in a syngeneic mouse model. Metabolic inhibition of sulfation by incubation of cells with sodium chlorate almost completely abrogated P-selectin binding. Metabolic labeling of MC-38 cells with (35)S sulfate revealed only a single band as detected by high-performance thin layer chromatography analysis of a total lipid extract. Matrix-assisted laser desorption/ionization tandem time-of-flight/time-of-flight analysis (MALDI-TOF-TOF) analysis of the purified sulfate-containing lipid fraction identified the selectin ligand to be a sulfated galactosylceramide SM4 (HSO(3)-3Galbeta-1Cer). Modulation of glycolipid biosynthesis in MC-38 cells altered P-selectin binding, thereby confirming sulfoglycolipids to be major P-selectin ligands. In addition, P-selectin was also found to recognize lactosylceramide sulfate SM3 (HSO(3)-3Galbeta-4Glcbeta-1Cer) and gangliotriaosylceramide sulfate SM2 [GalNAcbeta-4(HSO(3)-3)Galbeta-4Glcbeta-1Cer] in human hepatoma cells. Finally, the enzymatic removal of sulfation from the cell surface of MC-38 cells resulted in decreased P-selectin binding and led to attenuation of metastasis. Thus, SM4 sulfatide serves as a native ligand for P-selectin contributing to cell-cell interactions and to facilitation of metastasis.
ChemInform Abstract: Reaction of 2,4‐Di‐t‐butyl‐6‐methylphenylphosphonous Dichloride with Chloroform in the Presence of Lithium Diisopropylamide. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Improvements in the NIST Calibration Service for Thermal Transfer Standards (2007)

We report on multiple improvements made in the calibration services offered for thermal converters and thermal transfer standards in the ac-dc Difference Standards and Measurement Techniques Project at NIST during the past year. The major improvement in this calibration is the increased efficiency made possible by the consolidation of three disparate calibration services - low voltage thermal transfer standards, low- frequency thermal converters, and RF-dc difference calibrations - into a single service. This consolidation has the immediate benefit of offering NIST calibration customers one source for ac-dc calibrations from 2 mV to 1000 V and from 10 Hz to 1 GHz. Additional benefits include lower calibration prices and reduced uncertainties at many points.