gebhart/patrepeval huggingface dataset:
Subtype-selective expression of the five somatostatin receptors (hSSTR1-5) in human pancreatic islet cells: a quantitative double-label immunohistochemical analysis. (1999)

We have developed a panel of rabbit polyclonal antipeptide antibodies against the five human somatostatin receptor subtypes (hSSTR1-5) and used them to analyze the pattern of expression of hSSTR1-5 in normal human islet cells by quantitative double-label confocal fluorescence immunocytochemistry. All five hSSTR subtypes were variably expressed in islets. The number of SSTR immunopositive cells showed a rank order of SSTR1 > SSTR5 > SSTR2 > SSTR3 > SSTR4. SSTR1 was strongly colocalized with insulin in all beta-cells. SSTR5 was also an abundant isotype, being colocalized in 87% of beta-cells. SSTR2 was found in 46% of beta-cells, whereas SSTR3 and SSTR4 were relatively poorly expressed. SSTR2 was strongly colocalized with glucagon in 89% of alpha-cells, whereas SSTR5 and SSTR1 colocalized with glucagon in 35 and 26% of alpha-cells, respectively. SSTR3 was detected in occasional alpha-cells, and SSTR4 was absent. SSTR5 was preferentially expressed in 75% of SST-positive cells and was the principal delta-cell SSTR subtype, whereas SSTR1-3 were colocalized in only a few delta-cells, and SSTR4 was absent. These studies reveal predominant expression of SSTR1, SSTR2, and SSTR5 in human islets. Beta-cells, alpha-cells, and delta-cells each express multiple SSTR isoforms, beta-cells being rich in SSTR1 and SSTR5, alpha-cells in SSTR2, and delta-cells in SSTR5. Although there is no absolute specificity of any SSTR for an islet cell type, SSTR1 is beta-cell selective, and SSTR2 is alpha-cell selective. SSTR5 is well expressed in beta-cells and delta-cells and moderately well expressed in alpha-cells, and thereby it lacks the islet cell selectivity displayed by SSTR1 and SSTR2. Subtype-selective SSTR expression in islet cells could be the basis for preferential insulin suppression by SSTR1-specific ligands and of glucagon inhibition by SSTR2-selective compounds.
Model-projected mechanistic bases for sex differences in growth hormone regulation in humans (2006)

Models of physiological systems facilitate rational experimental design, inference, and prediction. A recent construct of regulated growth hormone (GH) secretion interlinks the actions of GH-releasing hormone (GHRH), somatostatin (SRIF), and GH secretagogues (GHS) with GH feedback in the rat (Farhy LS, Veldhuis JD. Am J Physiol Regul Integr Comp Physiol 288: R1649-R1663, 2005). In contrast, no comparable formalism exists to explicate GH dynamics in any other species. The present analyses explore whether a unifying model structure can represent species- and sex-defined distinctions in the human and rodent. The consensus principle that GHRH and GHS synergize in vivo but not in vitro was explicable by assuming that GHS 1) evokes GHRH release from the brain, 2) opposes inhibition by SRIF both in the hypothalamus and on the pituitary gland, and 3) stimulates pituitary GH release directly and additively with GHRH. The gender-selective principle that GH pulses are larger and more irregular in women than men was conferrable by way of 4) higher GHRH potency and 5) greater GHS efficacy. The overall construct predicts GHRH/GHS synergy in the human only in the presence of SRIF when the brain-pituitary nexus is intact, larger and more irregular GH pulses in women, and observed gender differences in feedback by GH and the single and paired actions of GHRH, GHS, and SRIF. The proposed model platform should enhance the framing and interpretation of novel clinical hypotheses and create a basis for interspecies generalization of GH-axis regulation.
Strained silver(i) coordination polymers of 1,4-diazatriphenylene (2003)

Two-dimensional coordination polymers of AgX (X = BF4−, ClO4−) and 1,4-diazatriphenylene show significant distortions of the polycyclic aromatic ligand and a new unsymmetrical η2-μ-η2 bonding mode for BF4− and ClO4−.
Visualizing contact line phenomena on microstructured superhydrophobic surfaces (2010)

The authors introduce a direct method for determining droplet solid-liquid-vapor interfacial geometry on microstructured surfaces. A heated liquid metal droplet of size 18 μl is deposited onto a microstructured surface and then freezes, preserving the microstructure impressions onto the metal surface. Postsolidification microscopy can measure contact line geometry and identify wetting state. This approach can be used to visualize contact line on curved and opaque microstructured surfaces.
AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice (2018)

Study of knockout (KO) mice has helped understand the link between many genes/proteins and human diseases. Identification of infertile KO mice provides valuable tools to characterize the molecular mechanisms underlying gamete formation. The KIAA0319L gene has been described to have a putative association with dyslexia; surprisingly, we observed that homozygous KO males for AU040320, KIAA0319L ortholog, are infertile and present a globozoospermia-like phenotype. Mutant spermatozoa are mostly immotile and display a malformed roundish head with no acrosome. In round spermatids, proacrosomal vesicles accumulate close to the acroplaxome but fail to coalesce into a single acrosomal vesicle. In wild-type mice AU040320 localises to the trans-Golgi-Network of germ cells but cannot be detected in mature acrosomes. Our results suggest AU040320 may be necessary for the normal formation of proacrosomal vesicles or the recruitment of cargo proteins required for downstream events leading to acrosomal fusion. Mutations in KIAA0319L could lead to human infertility; we screened for KIAA0319L mutations in a selected cohort of globozoospermia patients in which no genetic abnormalities have been previously identified, but detected no pathogenic changes in this particular cohort.
Double-Row Discrete-Hole Cooling: an Experimental and Numerical Study (1980)

Double-row discrete-hole cooling arrangements offer several advantages over single-row systems yet the detailed cooling mechanism is less completely understood than for the single-row. This is partly because there have been fewer studies of this geometry and partly because the flow structure is more complex. The present paper presents detailed flow-field and concentration measurements around the injection holes for double-row injection on a flat plate at 30 deg to the mainstream. The experiments span values of the blowing injection mass velocities from 0.25 to 1.0 times the free stream mass velocity and for two boundary layer thicknesses just upstream of the injection. In contrast to single-row injection the cooling effectiveness rise monotonically with M over the range studied. Computer simulation of these flows and similar experiments of [7] has been made using a three-dimensional finite-difference code that embodies a semi-elliptic treatment of the flow field in the neighborhood of the injection holes in conjunction with a two-equation turbulence model with non-isotropic effective transport coefficients. It emerged from the calculations, that, for injection velocities up to 50 percent of the free stream value, levels of film-cooling effectiveness are extremely well predicted beyond about 10 diameters behind the leading row of holes. Around the holes themselves, however, there are certain discrepancies which become more serious as the injection level is raised.
Growth and differentiation of alveolar bone cells in tissue‐engineered constructs and monolayer cultures (2008)

The ability to enhance bone regeneration by implanting autologous osteoblasts in combination with an appropriate scaffold would be of great clinical interest. The aim of our study was to compare the growth and differentiation of alveolar bone cells in tissue-engineered constructs and in monolayer cultures, as the basis for developing procedures for routine preparation of bone-like tissue constructs. Alveolar bone tissue was obtained from four human donors and explant cultures of the cells were established. Expanded cells were seeded on macroporous hydroxyapatite granules, and cultured in medium supplemented with osteogenic differentiation factors for up to 3 weeks. Control monolayer cultures were established in parallel, and cultured in media with or without osteogenic supplements. Cell proliferation, alkaline phosphatase (AP) activity and gene expression of AP, osteopontin and osteocalcin were determined under different culture conditions at weekly intervals. Cells in tissue constructs exhibited growth patterns similar to those in control monolayer cultures: enhanced proliferation was noted during the first 2 weeks of cultivation, followed by a decrease in cell numbers. AP activity at 3 weeks was higher in all cultures in osteogenic medium than in control medium. Gene expression levels were stable in monolayer cultures in both types of media whereas, in tissue constructs, they exhibited patterns of osteogenic differentiation. Light and scanning electron microscopy examination of the cell-seeded constructs showed uniform cell distribution, as well as cell attachment and growth into the interior region of the hydroxyapatite granules. Our results show that bone-like constructs with viable cells exhibiting differentiated phenotype can be prepared by cultivation of alveolar-bone cells on the tested hydroxyapatite granules.
Early and Transient Microchimerism Associated with Complete Remission after Adoptively Transferred Haploidentical NK Cells Against High Risk Myelodysplastic Syndrome and Refractory Acute Myeloid Leukemia (2014)

Abstract Introduction: We here report from an ongoing phase I/II study of HLA-haploidentical NK cell therapy to patients with high-risk myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) not eligible for standard therapies. The preparative regimen consisted of intermediate doses of Cyklophosphamide (Cy), Fludarabin (Flu) and titrated doses of total lymphoid irradiation (TLI). The trial design excluded systemic IL-2 treatment to avoid expansion of regulatory T cells and to test if in vivo expansion could be obtained without IL-2 support. Patients:The first 12 patients were treated with Cy/Flu and an escalating dose of TLI (2 Gy and 4 Gy), followed by infusion of short-term IL-2 activated (16 hours) NK cells. Three patients received daily cyclosporine A after the conditioning. Three had relapsed, chemotherapy-refractory, primary AML, seven had secondary relapsed or refractory MDS-AML and two had high risk MDS with fibrosis. Results: The treatment was well tolerated and no severe non-infectious toxicity could be observed in the patients. The endpoint of expansion (&gt;100 donor NK cells/ul at day 14) was not reached, but six patients had positive microchimerism, NK cells of donor origin detectable by RT-PCR at day 7-14, that thereafter became undetectable within 7-14 days. Four of these six patients achieved complete remission (CR) whereafter they become eligible for and could proceed to allogeneic stem cell transplantation. None of the patients with negative microchimerism obtained CR. Four patients died from progressive disease and three patients, with minor response and progressive disease, died in infections within three months of therapy. Discussion: Although the long-term efficacy needs to be evaluated, the results suggest that a combined regimen with mild conditioning followed by NK cell therapy may induce remission in patients with chemo-refractory disease and provide a bridge to allogeneic stem cell transplantation. Notably, clinical responses were observed after only a minimal in vivo NK cell expansion and were independent on KIR-ligand mismatch. Disclosures Blomberg: VECURA: Employment. Hellström-Lindberg:Celgene: Research Funding.
Spiropyrimidinetrione DNA Gyrase Inhibitors with Potent and Selective Antituberculosis Activity (2022)

This is the accepted manuscript version of the work published in its final form as Govender, P., Müller, R., Singh, K., Reddy, V., Eyermann, C. J., Fienberg, S., Ghorpade, S. R., Koekemoer, L., Myrick, A., Schnappinger, D., Engelhart, C., Meshanni, J., Byl, J. A. W., Osheroff, N., Singh, V., Chibale, K., &amp; Basarab, G. S. (2022). Spiropyrimidinetrione DNA Gyrase Inhibitors with Potent and Selective Antituberculosis Activity. <em>Journal of Medicinal Chemistry</em>, <em>65</em>(9), 6903-6925. https://doi.org/10.1021/acs.jmedchem.2c00266 Deposited by shareyourpaper.org and openaccessbutton.org. We've taken reasonable steps to ensure this content doesn't violate copyright. However, if you think it does you can request a takedown by emailing help@openaccessbutton.org.
Fast automatic elimination of vertical parallax of multiview images (2010)

A fast and automatic method is proposed to eliminate the vertical parallax in multiview images. The horizontal parallax provide the viewers stereoscopic feeling, but the vertical parallax causes the view discomfort in autostereoscopic display. To decrease the viewing discomfort, the elimination of vertical parallax is necessary. The complexity of traditional processing method is discussed, and the complexity of the current methods is very high. Thus it is difficult to do real time rectification of 3DTV video images. In the proposal, SIFT(Scale Invariant Feature Transform) is employed to detect the features among different views. And a compact processing procedure is proposed to lessen the complexity of traditional methods automatically without complicated parameters initialization. The experiment shows that the performance of the proposal is encouraging as well as the speed of the processing is fast. Thus, it is convenient to implement for real time 3D video acquisition and 3DTV displaying.
LinLogFS: a log-structured filesystem for Linux (2000)

LinLogFS is a log-structured filesystem for Linux. It currently offers features like fast crash recovery and in-order write semantics. We implemented LinLogFS by putting a logging layer between an adapted version of the ext2 file system and the block device.
Optical Phase Modulation Using Dual-Frequency Nematic Liquid Crystals (2008)

Phase modulation characteristics of dual-frequency liquid crystal materials possessing the dielectric anisotropy sign inversion have been studied in detail under the quasi-static and dynamic electric field control. It was found that the main obstacle in development of dual-frequency liquid crystal modulators having simultaneously short response time and large switchable values of phase retardation is related to an electrohydrodynamic instability, which disturbs the director reorientation. We are discussing general conditions at which a stable 2π phase modulation is possible at highest switching frequencies. In particular, for the developed dual-frequency liquid crystal material we have established conditions at which the stable phase retardation dynamic range of 2π at switching time ∼ 2 ms is realized at room temperature.
Deep-learning analysis of micropattern-based organoids enables high-throughput drug screening of Huntington’s disease models (2022)

Organoids are carrying the promise of modeling complex disease phenotypes and serving as a powerful basis for unbiased drug screens, potentially offering a more efficient drug-discovery route. However, unsolved technical bottlenecks of reproducibility and scalability have prevented the use of current organoids for high-throughput screening. Here, we present a method that overcomes these limitations by using deep-learning-driven analysis for phenotypic drug screens based on highly standardized micropattern-based neural organoids. This allows us to distinguish between disease and wild-type phenotypes in complex tissues with extremely high accuracy as well as quantify two predictors of drug success: efficacy and adverse effects. We applied our approach to Huntington's disease (HD) and discovered that bromodomain inhibitors revert complex phenotypes induced by the HD mutation. This work demonstrates the power of combining machine learning with phenotypic drug screening and its successful application to reveal a potentially new druggable target for HD.
A New Switching Technique for Direct Torque Control of Induction Motor using Four-Switch Three-Phase Inverter (2007)

This paper presents a new switching technique for direct torque control of induction motor using four-switch three phase inverter (DTC-FSTPI-IM) for low power applications. The modified switching table in this method is based on the principle of similarity between FSTPI and SSTPI (six-switch three phase inverter), where the αβ plan is divided into 6 sectors and the formation of the voltage space vector is done in the same way as for SSTPI by using effective (mean) vectors. This approach allows using the well-knowing established switching table of SSTPI for FSTPI, in order to reduce torque ripples in comparison with the conventional DTC method for FSTPI. The validity of new proposed technique is verified by simulation results using Matlab/Simulink and also experimentally by using DSpace 1104 Card.
Evaluation Of EL Display Technology For Portable Computer Applications (1985)

A 4x8 inch, 256x512 pixel EL display has been evaluated for use in a portable computer. The display has proven to have excellent visual qualities as well as good reliability. The main challenge now is for manufacturer&apos;s to get the cost down.
Characterization of 3D resist patterns by means of optical scatterometry (1999)

We report about our first attempts to apply optical scatterometry to the characterization of 3D resist patterns. Particularly, nominal quarter micron dots and holes are investigated having a pitch of a half micron in both directions. The specular reflected light versus the incidence angle shows significant variance with changing exposure dose and thus with changing dot or hole diameter, respectively. Scanning electron micrographs serve as an assisting tool for the characterization of the developed resist pattern. Additionally, an algorithm for the rigorous modeling of diffraction from regular 3D-patterns is sketched. In some cases, the match between experiment and theory is already quite satisfying. This might be improved by more refined profile adaption and enhanced computer power.
ChemInform Abstract: Synthesis and Stereochemistry of Indolactam‐(V), an Active Fragment of Teleocidins. Structural Requirements for Tumor‐Promoting Activity. (1987)

Abstract To determine the active structural fragment of the tumor‐promoting teleocidins and its absolute stereochemistry the four possible stereoisomers of the title compound (‐)‐(XIIIa) are synthesized starting from 4‐nitrogramine (I) and obtained as racemic mixtures.
Monitoring generators and large motors (1986)

The paper describes how techniques for monitoring the condition of generators and large motors have been used and developed in one region of the UK Central Electricity Generating Board. Many parameters need to be monitored on a generator, so that a comprehensive and clear picture of any degradation can be built up for use in planning shutdowns and maintenance. Only a few parameters are needed for motors. The criteria are given of experiences with selected monitoring techniques.
Disease- and headache-specific microRNA signatures and their predicted mRNA targets in peripheral blood mononuclear cells in migraineurs: role of inflammatory signalling and oxidative stress (2022)

We provide here the first evidence for disease- and headache-specific miRNA signatures in the PBMC of migraineurs, which might help to identify novel targets for both prophylaxis and attack therapy.
ChemInform Abstract: IMPEDANCE MEASUREMENTS AND PHOTOEFFECTS ON NICKEL ELECTRODES (1983)

Abstract Je nach Elektrodenpotential kann an der Oberfläche einer Ni‐Elektrode ein n‐ oder p‐leitendes Oxid vorhanden sein, was unter Bestrahlung anodische bzw. kathodische Photoströme bewirkt.
Recent advancements in structured-illumination microscopy toward live-cell imaging (2015)

Fluorescence microscopy allows us to observe fluorescently labeled molecules in diverse biological processes and organelle structures within living cells. However, the diffraction limit restricts its spatial resolution to about half of its wavelength, limiting the capability of biological observation at the molecular level. Structured-illumination microscopy (SIM), a type of super-resolution microscopy, doubles the spatial resolution in all three dimensions by illuminating the sample with a patterned excitation light, followed by computer reconstruction. SIM uses a relatively low illumination power compared with other methods of super-resolution microscopy and is easily available for multicolor imaging. SIM has great potential for meeting the requirements of live-cell imaging. Recent developments in diverse types of SIM have achieved higher spatial (∼50 nm lateral) and temporal (∼100 Hz) resolutions. Here, we review recent advancements in SIM and discuss its application in noninvasive live-cell imaging.
Identification of Quantitative Trait Loci Governing Arthritis Severity and Humoral Responses in the Murine Model of Lyme Disease (1999)

A spectrum of disease severity has been observed in patients with Lyme disease, with approximately 60% of untreated individuals developing arthritis. The murine model of Lyme disease has provided strong evidence that the genetic composition of the host influences the severity of arthritis following infection with Borrelia burgdorferi: infected C3H mice develop severe arthritis while infected C57BL/6N mice develop mild arthritis. Regions of the mouse genome controlling arthritis severity and humoral responses during B. burgdorferi infection were identified in the F2 intercross generation of C3H/HeNCr and C57BL/6NCr mice. Rear ankle swelling measurements identified quantitative trait loci (QTL) on chromosomes 4 and 5, while histopathological scoring identified QTL on a unique region of chromosome 5 and on chromosome 11. The identification of QTL unique for ankle swelling or histopathological severity suggests that processes under distinct genetic control are responsible for these two manifestations of Lyme arthritis. Additional QTL that control the levels of circulating Igs induced by B. burgdorferi infection were identified on chromosomes 6, 9, 11, 12, and 17. Interestingly, the magnitude of the humoral response was not correlated with the severity of arthritis in infected F2 mice. This work defines several genetic loci that regulate either the severity of arthritis or the magnitude of humoral responses to B. burgdorferi infection in mice, with implications toward understanding the host-pathogen interactions involved in disease development.
An mTOR kinase inhibitor slows disease progression in a rat model of polycystic kidney disease (2014)

In a rat model of autosomal dominant polycystic kidney disease, PP242 treatment (i) decreases proliferation in cystic and non-cystic tubules; (ii) inhibits renal enlargement and cystogenesis and (iii) significantly reduces the loss of kidney function.
The dependence of Schottky barrier potential on substrate orientation in PtSi infrared diodes (1990)

Platinum silicide Schottky barrier infrared diodes have been formed on p-type silicon substrates having both 〈100〉 and 〈111〉 orientations. The potential barrier to optically generated hot carriers has been measured and found to be 0.219 eV for 〈100〉 substrates and 0.313 eV for 〈111〉. Platinum-layer thickness was varied from 1 to 10 nm. The nearly 0.1 eV difference in Schottky barrier potential appears to depend only on the orientation of the silicon substrates.
ORAL VALGANCICLOVIR AND INTRAVENOUS GANCICLOVIR RESULTS IN COMPARABLE LONG-TERM OUTCOMES IN TRANSPLANT RECIPIENTS WITH CMV DISEASE: THE VICTOR STUDY (2008)

Åberg, A; Pescovitz, M D.; Humar, A; Jardine, A G.; Rollag, H; Mouas, H; Hartmann, A Author Information