gebhart/patrepeval huggingface dataset:
Rapid Detection of Human Pathogenic Orthobunyaviruses (2003)

Modern detection and identification tools can help to provide answers to urgent questions about the incidence, prevalence, and epidemiology of currently emerging diseases. We developed highly sensitive one-step TaqMan reverse transcription-PCR assays with sensitivities ranging from 10(4) to 10(1) molecules for 11 human pathogens of the orthobunyaviruses. We compared the performances of these assays on three currently available cyclers (ABI-PRISM 7700, LightCycler, and SmartCycler). The assay for Oropouche virus (OROV) was tested using sera collected from days 1 to 5 after onset of OROV disease and was found to be greatly superior to an established nested PCR system. A mean copy number of 1.31 x 10(7) OROV RNA/ml of serum was detected. Diagnostic RNA detection can be used as early as day 1 after onset of OROV disease. The use of a mobile SmartCycler and a hands-on time of less than 3 h could help to intensify outbreak surveillance and control, especially in field studies.
Microstructural Evolution of Calcium‐Doped α‐Alumina (2003)

Effect of different calcium doping levels on the microstructure of high‐purity α‐alumina was studied as a function of sintering time and temperature using scanning electron microscopy (SEM). Microstructural evolution was related to hypothetical calcium excess at the grain boundaries (Γ Ca ) that was calculated assuming zero solubility of calcium in bulk α‐alumina. Under all sintering conditions, grains were uniform in size and equiaxed for low calcium concentrations (&lt;3 Ca atoms/nm 2 ). The grain morphology became elongated when the calcium concentration at the grain boundaries reached calcium excess of Γ Ca = 3–3.5 Ca atoms/nm 2 in all samples. The average grain sizes of undoped samples were ∼10% larger than the average grain sizes of low‐calcium‐doped samples. This decrease is believed to be due to solute drag effect of segregated Ca impurities on the grain boundary mobility. For the samples that were sintered at 1500° and 1600°C, slablike abnormally grown grains appeared for critical calcium excess concentrations of Γ Ca = 4.5–8 Ca atoms/nm 2 . With abnormally grown grains a dramatic increase in average grain size was observed. However, when the calcium concentration was increased further, above certain calcium excess concentration depending on sintering temperature, a significant decrease in grain size was observed. In contrast to samples sintered at 1500° and 1600°C, when the samples sintered at 1400°C, although the hypothetical calcium coverage exceeded Γ Ca = 11 Ca atoms/nm 2 , only few grains grew abnormally without significantly affecting the average grain size. Observations clearly indicated that calcium impurities caused elongated (slablike) grain morphology when their excess concentrations reached a critical level at the grain boundaries.
Design and formulation of mucoadhesive microspheres of sitagliptin (2012)

Mucoadhesive microspheres of sitagliptin (SITCM), a new anti-diabetic drug was prepared with carbopol 934 P using Buchi B-90 nano spray drier and optimized to analyse the key effects and relations of three factors on formulation of SITCM were studied. The appearance of the microspheres was found to be shriveled to nearly spherical, with a narrow size of 2-8 µm. The drug loading and percentage yield was found to be 73 ± 0.2% and 92 ± 0.3%, respectively. In vitro release indicated Korsmeyer-Peppas pattern mucoadhesion of SITCM-8 was found to be 7.8 ± 0.3 h. In vivo studies in rats suggest that the sitagliptin was retained in the gastrointestinal tract for an extended period of time (∼12 h) and control group was reduced significantly (∼4 h). This study concludes that the mucoadhesive microsphere could be one of the most appropriate drug delivery approaches for the successful delivery of sitagliptin.
Natural Attenuation of Explosives in Soil and Water Systems at Department of Defense Sites (1999)

Abstract : This report, which is a follow-on to the report Natural Attenuation of Explosives in Groundwater at DoD Sites: Interim Report, presents results of a study to evaluate the feasibility of using stable isotopes of carbon and nitrogen in soil or groundwater to monitor natural attenuation of explosives. The report also includes continuation results from two research areas, toxicity testing of surface soils at the demonstration site and integration of biomarker data into the model 3DFATMIC (3-Dimensional Subsurface Flow, Fate, and Transport of Microbes and Chemicals). Results of stable isotope studies indicated that changes in carbon isotope ratios were too small to detect. Therefore, this approach is not feasible. However, changes in nitrogen ratios in TNT found in groundwater were detected across the contaminant plume. Once the rate and magnitude of these changes are related quantitatively to TNT attenuation rates, this approach may be feasible for monitoring attenuation processes. In toxicity tests, the significant negative correlation between TNT concentration and viable biomass in aquifer soils at the Louisiana Army Ammunition Plant, Doyline, LA, could not be related to toxicity of TNT to the indigenous nicroflora. In the modeling research area, the 3DFAIMIC model exhibited a problem in the numerical code, and the amount of biomass data proved too small. Predictive simulations may be possible with the model once the code is revised and additional biomass data become available.
Nematic-Cholesteric Relaxation in some Cholesteric Liquid Crystals (1983)

Abstract It is well known that a cholesteric liquid crystal of positive dielectric or diamagnetic anisotropy can be transformed into an aligned nematic with the director parallel to the field, by an electric or magnetic field higher than a threshold value. Such a phase transition corresponds to a cholesteric helix unwinding with an intermediate state of focal conic structure which is optically cloudy, whereas the nematic is transparent. This phase transformation has been currently used in display devices during the last few years.
Interaction of α Carboxyl Terminus 1 Peptide With the Connexin 43 Carboxyl Terminus Preserves Left Ventricular Function After Ischemia‐Reperfusion Injury (2019)

Background α Carboxyl terminus 1 (αCT1) is a 25-amino acid therapeutic peptide incorporating the zonula occludens-1 (ZO-1)-binding domain of connexin 43 (Cx43) that is currently in phase 3 clinical testing on chronic wounds. In mice, we reported that αCT1 reduced arrhythmias after cardiac injury, accompanied by increases in protein kinase Cε phosphorylation of Cx43 at serine 368. Herein, we characterize detailed molecular mode of action of αCT1 in mitigating cardiac ischemia-reperfusion injury. Methods and Results To study αCT1-mediated increases in phosphorylation of Cx43 at serine 368, we undertook mass spectrometry of protein kinase Cε phosphorylation assay reactants. This indicated potential interaction between negatively charged residues in the αCT1 Asp-Asp-Leu-Glu-Iso sequence and lysines (Lys345, Lys346) in an α-helical sequence (helix 2) within the Cx43-CT. In silico modeling provided further support for this interaction, indicating that αCT1 may interact with both Cx43 and ZO-1. Using surface plasmon resonance, thermal shift, and phosphorylation assays, we characterized a series of αCT1 variants, identifying peptides that interacted with either ZO-1-postsynaptic density-95/disks large/zonula occludens-1 2 or Cx43-CT, but with limited or no ability to bind both molecules. Only peptides competent to interact with Cx43-CT, but not ZO-1-postsynaptic density-95/disks large/zonula occludens-1 2 alone, prompted increased pS368 phosphorylation. Moreover, in an ex vivo mouse model of ischemia-reperfusion injury, preischemic infusion only with those peptides competent to bind Cx43 preserved ventricular function after ischemia-reperfusion. Interestingly, a short 9-amino acid variant of αCT1 (αCT11) demonstrated potent cardioprotective effects when infused either before or after ischemic injury. Conclusions Interaction of αCT1 with the Cx43, but not ZO-1, is correlated with cardioprotection. Pharmacophores targeting Cx43-CT could provide a translational approach to preserving heart function after ischemic injury.
LCAA, a Novel Factor Required for Magnesium Protoporphyrin Monomethylester Cyclase Accumulation and Feedback Control of Aminolevulinic Acid Biosynthesis in Tobacco (2012)

Low Chlorophyll Accumulation A (LCAA) antisense plants were obtained from a screen for genes whose partial down-regulation results in a strong chlorophyll deficiency in tobacco (Nicotiana tabacum). The LCAA mutants are affected in a plastid-localized protein of unknown function, which is conserved in cyanobacteria and all photosynthetic eukaryotes. They suffer from drastically reduced light-harvesting complex (LHC) contents, while the accumulation of all other photosynthetic complexes per leaf area is less affected. As the disturbed accumulation of LHC proteins could be either attributable to a defect in LHC biogenesis itself or to a bottleneck in chlorophyll biosynthesis, chlorophyll synthesis rates and chlorophyll synthesis intermediates were measured. LCAA antisense plants accumulate magnesium (Mg) protoporphyrin monomethylester and contain reduced protochlorophyllide levels and a reduced content of CHL27, a subunit of the Mg protoporphyrin monomethylester cyclase. Bimolecular fluorescence complementation assays confirm a direct interaction between LCAA and CHL27. 5-Aminolevulinic acid synthesis rates are increased and correlate with an increased content of glutamyl-transfer RNA reductase. We suggest that LCAA encodes an additional subunit of the Mg protoporphyrin monomethylester cyclase, is required for the stability of CHL27, and contributes to feedback-control of 5-aminolevulinic acid biosynthesis, the rate-limiting step of chlorophyll biosynthesis.
Coating of pharmaceutical powders by fluidized bed process. IV. Softening temperature of acrylic copolymers and its relation to film-formation in aqueous coating. (1988)

The softening temperature (Ts) of films of commercially available and newly developed acrylic copolymers for aqueous coating was measured by thermomechanical analysis. The results are discussed in relation to the film-formation of their latices in the Wurster process.Methacrylic acid (MA)-ethyl acrylate (EA)(1: 1) copolymer dispersion (Eudragit L30D-55) containing 10% triacetin exhibited excellent film-forming ability in coating at a temperature (31°C) far lower than its Ts (91°C). The product exhibited no stickiness during drying at 60°C. The dissolution properties were little changed by heating at 60°C.On the other hand, the copolymer dispersion consisting of hydrophobic acrylic esters and minor quaternary ammonium esters (Eudragit RS30D, Ts=83°C) did not form a film under the same coating conditions in spite of its Ts being lowered to 49°C by the addition of 10% triacetin. The product was sticky during drying at 60°C. The copolymer consisting of only hydrophobic esters (Eudragit E3OD) had a good film-forming ability due to its low Ts (18°C), but the product was too sticky to be discrete particles even at room temperature.These results suggested that hydrophilic residues such as carboxyl and hydroxyl contribute to film-formation of the latices.
Highly effective interlayer expanded MoS2 coupled with Bi2WO6 as p-n heterojunction photocatalyst for photodegradation of organic dye under LED white light (2023)

The construction of a p-n heterojunction photocatalyst manage to conquer the setbacks of low photocatalytic activity caused by rapid charge recombination and precarious photo-response range. Herein, an interlayer of expanded MoS 2 coupled with Bi 2 WO 6 (BW-IEM) photocatalyst was synthesized for removal of Methylene Blue (MB) up to 97% (5.18 ×10 −2 min −1 ) under irradiation of low power 1 W LED white light. The outstanding photocatalytic performance of the p-n heterojunctions BW-IEM photocatalyst is mainly attributed to the enhancement of light harvesting, improvement in the separation of charge carriers, and coexistence of oxygen vacancy . The main reactive oxygen species (ROS) responsible for the photodegradation of MB were •O 2 - radicals and • OH. This work provides a new strategy for the potential IEM photocatalyst as a matrix in a p-n heterojunction composite. • p-n heterojunctions of interlayer expanded MoS 2 coupled with Bi 2 WO 6 . • 4% BW-IEM shows excellent LED white light driven photocatalytic activity . • Bi 2 WO 6 and oxygen vacancy enhances the separation of photogenerated carries. • • O 2 - and • OH radicals are the main reactive oxygen species.
Technology requirements of the 3GPP-TDD terminal (2000)

This paper examines the technology requirements for the implementation of the 3GPP-TDD mode and discusses how the terminal complexity of the TDD mode compares to that of the FDD mode. An overview of the TDD mode covers the frequency bands, the synchronous nature, and the physical channel structure. The differences in the inner receiver implementation between the TDD mode and the FDD mode are highlighted. The paper identifies the key complexity issues that determine the TDD terminal architecture. It explains the requirement for terminal synchronisation to the network in the synchronous TDD mode and asynchronous FDD mode. The description of the baseband implementation covers the inner receiver functions of the TDD mode: channel estimation, active code detection and multi-user detection. The latter is basically examined from the joint detection point of view, nevertheless other techniques are presented with particular interest in the single detection algorithms. This leads to a comparison of the terminal architectures for the FDD and TDD modes, in particular the suitability of the air interface to flexible implementation on a DSP.
Microbial symbiosis and coevolution of an entire clade of ancient vertebrates: the gut microbiota of sea turtles and its relationship to their phylogenetic history (2020)

This is one of the most comprehensive microbiota studies to have been performed in a single clade of animals and further improves our knowledge of how microbial populations have influenced vertebrate evolution.
Optimización multiobjetivo de una planta de desalación solar con almacenamiento de energía (2013)

La integracion del uso de energias renovables en procesos industriales es un area de creciente interes. En este trabajo presentamos el diseno optimo de una planta de desalacion que combina osmosis inversa, ciclo Rankine, colectores cilindricos parabolicos y almacenamiento de energia termica. Los colectores transforman la energia solar en energia termica que se utiliza en el ciclo Rankine, mientras que se utiliza la electricidad generada en las bombas de alta presion. El almacenamiento de energia termica permite adaptarse a la variabilidad de la radiacion solar, posibilitando un mayor aprovechamiento de la energia solar debido a la flexibilidad que proporciona. El diseno integra una caldera de combustible fosil como sistema de apoyo. El problema de diseno se ha planteado con un modelo de programacion multi-objetivo no lineal entero mixto (MINLP). Se optimiza simultaneamente el diseno y las condiciones de operacion considerando aspectos economicos y medio-ambientales. El impacto ambiental se evalua siguiendo la metodologia del analisis de ciclo de vida (LCA), utilizando el indicador CML 2001. Este procedimiento permite la cuantificacion de los impactos considerando todas las etapas del ciclo de vida. La solucion se presenta en forma de una frontera de Pareto, donde cada punto corresponde a una unica combinacion, por lo que se obtiene la solucion optima entre los criterios economicos y ambientales. Por falta de espacio este trabajo presenta los aspectos mas relevantes. Para detalles sobre la metodologia damos las correspondientes referencias que incluyen toda la informacion relevante. Los resultados muestran que la combinacion de desalacion con colectores solares de alta temperatura y el almacenamiento de energia termica se pueden lograr mejoras ambientales significativas, cuantificando el coste asociado.
Attosecond Soft-X-Ray Spectroscopy of a Transition Metal Dichalcogenide Material (2019)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text B. Buades, I. Leon, N. Di Palo, D. Rivas, T. P. H. Sidiropolous, S. Severino, M. Reduzzi, S. Cousin, M. Hemmer, C. Cocchi, E. Pellegrin, J. H. Martin, S. Mañas-Valero, E. Coronado, T. Danz, C. Draxl, M. Uemoto, K. Yabana, M. Schultze, S. Wall, A. Picon, and J. Biegert, "Attosecond Soft-X-Ray Spectroscopy of a Transition Metal Dichalcogenide Material," in Nonlinear Optics (NLO), OSA Technical Digest (Optica Publishing Group, 2019), paper NF1A.5. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
An Open-Label Phase Ib Dose-Escalation Study of TRC105 (Anti-Endoglin Antibody) with Bevacizumab in Patients with Advanced Cancer (2014)

TRC105 was well tolerated with bevacizumab and clinical activity was observed in a VEGF inhibitor-refractory population. Ongoing clinical trials are testing TRC105 in combination with bevacizumab in glioblastoma and with VEGFR TKIs in renal cell carcinoma, hepatocellular carcinoma, and soft tissue sarcoma.
Essais d'expansion pressiométriques avec mesure de pression interstitielle – application sur le site du Larivot (2022)

International audience
Establishment and characterization of three new rat renal cell carcinoma cell lines from N‐ethyl‐N‐hydroxyethylnitrosamine‐induced basophilic cell tumors (2001)

Three new rat cell lines (designated as BP13, BP30 and BP36B), derived from rat basophilic-type renal cell carcinomas induced with N-ethyl-N-hydroxyethylnitrosamine, were established and characterized. Passaged up to 100 times in vitro for 3 years, each cell line forms epithelial monolayers with cell cycles for BP13, BP30 and BP36B of 29, 21 and 17 h, respectively. Positive glucose-6-phosphate dehydrogenase (G6PD) and gamma-glutamyltransferase (gamma-GT) activity in their cytoplasm, but negative succinate dehydrogenase (SD) and slightly positive carbonic anhydrase type II (CA) localization indicates an origin from proximal tubules. Ultrastructural examination showed the presence of variable numbers of mitochondria and many microvilli and intracellular junctions on the plasma membrane. BP13 and BP30 were found to be tetraploid and BP36B diploid. BP13 has one marker chromosome 15p+, and BP36B an isochromosome of 1q. Anchorage-independent growth and tumorigenicity in immunosuppressed nude mice of BP13 and BP36B, but not BP30, proved their neoplastic nature. These three cell lines should provide useful tools for studying the biological characteristics of renal cell tumors.
Safety and Efficacy Review of Doxycycline (2009)

Doxycycline is a member of the tetracycline class of antibiotics and has been used clinically for more than 40 years. It is a well-tolerated drug that is bacteriostatic and acts via the inhibition of bacterial ribosomes. It is generally given at a dose of 100-mg daily or twice daily. It is well absorbed and has generally good tissue penetration. The serum half-life is 18-22 hours and dosage does not need to be adjusted in the presence of renal or hepatic impairment. Major side effects are gastro-intestinal and dermatological and it is generally contra-indicated in pregnancy or childhood because of concerns about discolouration of developing teeth and potential effects on growing bones. Drug interactions are not common although can occur with the concomitant use of methotrexate and the oral contraceptive pill, and its absorption can be reduced by the co-administration with some antacids and iron preparations. It has activity against many organisms, including Gram-positives, Gram-negatives and atypical bacteria. In addition, it appears to have some potentially clinically useful anti-inflammatory properties.
Precious Metal Core−Shell Spindles (2007)

A simplified method to produce spindle-shaped particles with a hematite core and a silica shell is described. The silica shell can, in turn, serve as the substrate for an outer coating of Ag or Au nanoparticles. The resulting multilayer core−shell particles display a flexible optical extinction spectrum, due primarily to the sensitivity of their plasmon resonance to the morphology of the precious metal outer coating.
Capability of single hardware channel for automotive safety applications according to ISO 26262 (2012)

There is no doubt that electromobility will be the future. All-electric vehicles were already available on the market in 2011 and 14 new vehicles will be commercially available in 2012. Due to the fact that automotive applications are influenced by the safety requirements of the ISO 26262, nowadays the use of new technologies requires more and more understanding for fail-safe and fault-tolerant systems due to increasingly complex systems. The safety of electric vehicles has the highest priority because it helps contribute to customer confidence and thereby ensures further growth of the electromobility market. Therefore in series production redundant hardware concepts like dual core microcontrollers running in lock-step-mode are used to reach ASIL D requirements given from the ISO 26262. In this paper redundant hardware concepts and the coded processing will be taken into account, which are listed in the current standard ISO 26262 as recommended safety measures.
The use of non- treated starch for butanol production by Clostridium acetobutylicum (2015)

Introduction: Greenhouse effect problems, environmental pollution, global increase in oil demand, and reduced fossil fuel resources have boosted research on the production of renewable energies such as bioenegries in recent years. Amongst various biofuels, biobutanol has been recently introduced as a replacement liquid fuel for gasoline and gas oil. Anaerobic bacteria such as Clostridium acetobutylicum are able to produce acetone, butanol, and ethanol using different sugar sources. This bacterium exhibits amylolytic activity and therefore is able to hydrolyzes starch to glucose and use it directly as carbon source. Materials and methods: In this study, three substrates including glucose, treated starch and starch were used in different concentrations to produce butanol by Clostridium acetobutylicum PTCC 1492. Results: For all three carbon sources, 60 g/ l of substrate was found as the optimum concentration. The results revealed that this bacterium is capable of producing butanol using all three carbon sources without any treatment. Butanol concentrations of 6.45, 5.81, and 4.64 g/ l were obtained using non-treated starch, treated starch, and glucose as carbon sources, respectively. Discussion and conclusion: The results suggested the possibility of using non-hydrolyzed starch as carbon source for butanol production. Also it was shown that non-treated starch produces more butanol compared to the treated starch.