gebhart/patrepeval huggingface dataset:
HMG box proteins in early T-cell differentiation. (1993)

The central theme of this review is the molecular basis for commitment of cells to the T-cell lineage. Principles of transcriptional regulation are illustrated by two examples; the role of GATA-1 during erythroid differentiation and the function of MyoD-like proteins in myogenesis. Several regulatory proteins have been described in the T-cell lineage. Here, we focus attention on the HMG box family of DNA binding proteins. This recently defined family can be divided in two subfamilies: the HMG/UBF and the TCF/SOX group. The first group contains at least two HMG boxes and binds DNA non-specifically, while the other group of proteins has one HMG box and interacts with DNA sequence-specifically. Characteristics of the most prominent members of both subfamilies will be discussed. In particular, we will address the role of HMG box proteins in controlling the expression of T-cell specific proteins during differentiation.
Phosphorylation of the Cytoplasmic Domain of E-Selectin Is Regulated During Leukocyte-Endothelial Adhesion (1998)

E-selectin, a selectin expressed on activated vascular endothelium, supports rolling and stable adhesion of leukocytes at sites of inflammation. Previously, we have reported that leukocyte adhesion to cultured endothelial cells induces association of the cytoplasmic domain of E-selectin with cytoskeletal elements, suggesting that outside-in signaling may occur during E-selectin-mediated adhesion. To investigate this potential signaling function of E-selectin, HUVEC activated with recombinant human IL-1beta (10 U/ml, 4 h) were labeled with [32P]orthophosphate, and E-selectin was immunoprecipitated using mAb H18/7. Autoradiography revealed constitutive phosphorylation of E-selectin in these cells and time-dependent dephosphorylation following adhesion of HL-60 cells. Cross-linking of cell surface E-selectin using H18/7 and a polyclonal secondary Ab induced E-selectin dephosphorylation, as did adhesion of beads coated with recombinant P-selectin glycoprotein ligand-1 (PSGL-1), an E-selectin ligand. Using adenoviral vector-mediated transfection in HUVEC of a tail-less E-selectin and phosphoamino acid analysis, we documented phosphorylation occurring exclusively within the cytoplasmic domain and involving serine residues. Additional experiments using a series of cytoplasmic domain mutants of E-selectin expressed in COS-7 cells localized the regions that were constitutively phosphorylated. Preincubation with okadaic acid and sodium vanadate abrogated adhesion-induced dephosphorylation of E-selectin. Thus, E-selectin, which is constitutively phosphorylated in cytokine-activated human endothelial cells, undergoes an enzymatically regulated dephosphorylation following leukocyte adhesion. This process appears to be triggered by multivalent ligand binding and/or cross-linking of cell surface E-selectin. Ligand-dependent regulation of the phosphorylation of E-selectin's cytoplasmic domain provides additional evidence for a transmembrane signaling function of this molecule during leukocyte-endothelial interactions.
The coordination chemistry of phenylbisdimethylaminophosphine oxide (1971)

Abstract A number of new solvates and adducts containing phenylbisdimethylaminophosphine oxide is reported. The solvates have the general formula M{C 6 H 5 P(O)‐[N(CH 3 ) 2 ] 2 } 4 2+ (anion) 2 2− , in which M = Mg, Ca, Mn, Fe, Co, Ni, Cu, Zn and Cd, and the anions are ClO 4 − and BF 4 − . The adducts have the general formula MCl 2 {C 6 H 5 P(O)[N(CH 3 ) 2 ] 2 } n , in which M = Mg, Ca, Mn, Co, Ni, Cu, Zn and Cd, and n = 2, except for the Cd compound in which n = 3. The compounds are characterized and identified by chemical analyses and physical measurements. Vibrational spectra in the region 4000‐200 cm −1 have been investigated. A tentative assignment is proposed and the shifts of some ligand bands upon complexing are discussed. It is concluded that coordination takes place via the oxygen atom. Ligand‐field spectra for the complexes of Mn(II), Fe(II), Co(II), Ni(II) and Cu(II) have been investigated. Values for the ligand‐field parameters are reported. X‐ray powder patterns were used in combination with ligand‐field spectra to deduce the coordination around the metal ions. By a comparison of the ligand properties of phenylbisdimethylaminophosphine oxide, hexamethylphosphoramide and triphenylphosphine oxide the importance of steric factors in coordination chemistry is again demonstrated.
Zn‐Porphyrin‐Sensitized Nanocrystalline TiO2 Heterojunction Photovoltaic Cells (2005)

Solar cells: Novel green zinc metalloporphyrins (Zn-1 and Zn-2, see picture) were scrutinized for conversion of sunlight into electricity by anchoring the dyes to nanocrystalline TiO2 films and incorporating these as electrodes into solid-state photovoltaic devices. Under standard AM 1.5 sunlight, the Zn-1 and Zn-2 sensitized solar cells exhibited overall conversion efficiencies of 2.5 % and 3.0 %, respectively (IPCE=incident photon-to-current conversion efficiency).
Study of Early Stages in the Growth of Boron‐Doped Diamond on Carbon Fibers (2020)

With the aim to improve the properties of aeronautical carbon fiber (CF)‐reinforced polymer materials, boron‐doped diamond (BDD) has been grown on commercial CFs by microwave plasma‐enhanced chemical vapor deposition. The growth of a BDD layer on CF is expected to increase the mechanical, electrical, and thermal conductivities of the composite. The present contribution reports the different growth stages and their associated nanostructures of the BDD/CF resulting system using transmission electron microscopy, scanning electron microscopy, and Raman spectroscopy. The revealed carbon nanostructure shows four steps of growth with the following characteristics: 1) transitional interlayer, 2) graphite nanowall, 3) cauliflower‐like nanocrystalline diamond, and 4) microcrystalline diamond.
Controlled freezing and freeze drying: a versatile route for porous and micro‐/nano‐structured materials (2010)

Abstract Freeze drying is a process whereby solutions are frozen in a cold bath and then the frozen solvents are removed via sublimation under vacuum, leading to formation of porous structures. Pore size, pore volume and pore morphology are dependent on variables such as freeze temperature, solution concentration, nature of solvent and solute, and the control of the freeze direction. Aqueous solutions, organic solutions, colloidal suspensions, and supercritical CO 2 solutions have been investigated to produce a wide range of porous and particulate structures. Emulsions have recently been employed in the freeze drying process, which can exert a systematic control on pore morphology and pore volume and can also lead to the preparation of organic micro‐ and nano‐particles. Spray freezing and directional freezing have been developed to form porous particles and aligned porous materials. This review describes the principles, latest progress and applications of materials prepared by controlled freezing and freeze drying. First of all the basics of freeze drying and the theory of freezing are discussed. Then the materials fabricated by controlled freezing and freeze drying are reviewed based on their morphologies: porous structures, microwires and nanowires, and microparticles and nanoparticles. The review concludes with new developments in this area and a brief look into the future. Copyright © 2010 Society of Chemical Industry
A solid state ultrabright source of entangled photon pairs (2010)

We demonstrate the fabrication of highly efficient sources of entangled photon pairs by inserting a semiconductor
quantum dot in an optical cavity. Two electron-hole pairs trapped in a quantum dot (QD) radiatively recombine emitting
a cascade of two polarization entangled photons. To extract both photons, we use a photonic molecule consisting of two
identical micropillars, one empty, the other embedding a chosen QD. By adjusting the diameter of the pillars and their
relative distance, we ensure that both optical transitions of the QD are simultaneously resonant to cavity modes. The
emitted photon pairs are efficiently extracted thanks to Purcell effect. Doing so, we obtain the brightest sources of
entangled photon pairs to date. We further show that the implementation of Purcell effect allows increasing the fidelity
of the two photon state by reducing spin induced phase shift during the radiative cascade.
Detection of static and dynamic components of mechanical allodynia in rat models of neuropathic pain: are they signalled by distinct primary sensory neurones? (1999)

In the present study, chronic constrictive injury (CCI model) of the sciatic nerve or tight ligation of L5 and L6 spinal nerves (Chung model) produced both dynamic and static components of mechanical allodynia in rats. The two responses were detected, respectively, by lightly stroking the hind paw with cotton wool or application of pressure using von Frey hairs. Animals with spinal nerve ligation developed both types of responses at a faster rate compared to animals with the CCI. Morphine (1-3 mg/kg, s.c.) dose-dependently blocked static but not dynamic allodynia. In contrast, pregabalin (previously S-isobutylgaba and CI-1008) dose-dependently (3-30 mg/kg, p.o.) blocked both types of allodynia. In CCI animals, two administrations of capsaicin (100 microg/50 microl) into the plantar surface of the ipsilateral paw at 1-h intervals blocked the maintenance of thermal hyperalgesia without affecting either static or dynamic allodynia. The similar administration of a further two doses of capsaicin into the same animals blocked the maintenance of static allodynia without affecting the dynamic response. These data indicate that thermal hyperalgesia, static and dynamic allodynia are respectively signalled by C-, Adelta- and Abeta/capsaicin insensitive Adelta- primary sensory neurones. It is suggested that pregabalin possesses a superior antiallodynic profile than morphine and may represent a novel class of therapeutic agents for the treatment of neuropathic pain.
The Effect of the Treatment with Heated Humidified High-Flow Nasal Cannula on Neonatal Respiratory Distress Syndrome in China: A Single-Center Experience (2017)

<i>Background</i>. Noninvasive respiratory support is considered the optimal method of providing assistance to preterm babies with breathing problems, including nasal continuous positive airway pressure (NCPAP) and humidified high flow nasal cannula (HHHFNC). The evidence of the efficacy and safety of HHHFNC used as the primary respiratory support for respiratory distress syndrome (RDS) is insufficient in low- and middle-income countries. <i>Objective</i>. To investigate the effect of heated humidified high flow nasal cannula on neonatal respiratory distress syndrome compared with nasal continuous positive airway pressure. <i>Methods</i>. An observational cross-sectional study was performed at a tertiary neonatal intensive care unit in suburban Wenzhou, China, in the period between January 2014 and December 2015. <i>Results</i>. A total of 128 infants were enrolled in the study: 65 in the HHHFNC group and 63 in the NCPAP group. The respiratory support with HHHFNC was similar to that with NCPAP with regard to the primary outcome. There is no significant difference between two groups in secondary outcomes. Comparing with NCPAP group, the incidence of nasal damage was lower in HHHFNC group. <i>Conclusions</i>. HHHFNC is an effective and well-tolerated strategy as the primary treatment of mild to moderate RDS in preterm infants older than 28 weeks of GA.
Influence of small rings on the thermodynamics of equilibrium self-assembly (2012)

The competition between the formation of linear chain clusters and ring structures in an equilibrium self-assembling system is reexamined by developing a new Flory-Huggins type theory that combines an estimate for the loss of configurational entropy ΔS(ring) upon ring formation with the standard treatment of the free energy of a polydisperse solution of linear chains. The excess entropy of ring formation ΔS(ring) is obtained from an analytical fit to exact enumeration data for self-avoiding chains and rings with 30 or fewer steps on a cubic lattice. Illustrative calculations of the spinodal curves and the extent and the average degree of self-assembly highlight the physical conditions for which the cyclic structures impact the thermodynamic characterization of equilibrium self-assembling systems.
High-Throughput Genetic Screen for Synaptogenic Factors: Identification of LRP6 as Critical for Excitatory Synapse Development (2013)

Genetic screens in invertebrates have discovered many synaptogenic genes and pathways. However, similar genetic studies have not been possible in mammals. We have optimized an automated high-throughput platform that employs automated liquid handling and imaging of primary mammalian neurons. Using this platform, we have screened 3,200 shRNAs targeting 800 proteins. One of the hits identified was LRP6, a coreceptor for canonical Wnt ligands. LRP6 regulates excitatory synaptogenesis and is selectively localized to excitatory synapses. In vivo knockdown of LRP6 leads to a reduction in the number of functional synapses. Moreover, we show that the canonical Wnt ligand, Wnt8A, promotes synaptogenesis via LRP6. These results provide a proof of principle for using a high-content approach to screen for synaptogenic factors in the mammalian nervous system and identify and characterize a Wnt ligand receptor complex that is critical for the development of functional synapses in vivo.
Superparamagnetic Iron Oxide–Enhanced MRI of Atherosclerotic Plaques in Watanabe Hereditable Hyperlipidemic Rabbits (2000)

SPIO accumulates in aortic plaques of atherosclerotic rabbits, producing a characteristic MRI finding. As SPIO accumulates in plaques with increased endothelial permeability and a high macrophage content, two established features of plaque inflammation, it may have a potential for noninvasive assessment of inflammatory atherosclerotic plaques.
Perspectives on genome‐wide multi‐stage family‐based association studies (2011)

With the establishment of large consortiums of researchers, genome-wide association (GWA) studies have become increasingly popular and feasible. Although most of these association studies focus on unrelated individuals, a lot of advantages can be exploited by including families in the analysis as well. To overcome the additional genotyping cost, multi-stage designs are particularly useful. In this article, I offer a perspective view on genome-wide family-based association analyses, both within a model-based and model-free paradigm. I highlight how multi-stage designs and analysis techniques, which are quite popular in clinical epidemiology, can enter GWA settings. I furthermore discuss how they have proven successful in reducing analysis complexity, and in overcoming one of the most cumbersome statistical hurdles in the genome-wide context, namely controlling increased false positives due to multiple testing.
A hybrid density functional study of lithium in ZnO: Stability, ionization levels, and diffusion (2009)

The properties of interstitial and substitutional Li in wurtzite ZnO are modeled using hybrid density functional calculations. We investigate the impact of the band-gap error on the formation energies of the two defects and their dependence on the Fermi level. It is found that within a local-density approximation, the acceptor level of ${\text{Li}}_{\text{Zn}}$ is very close to the valence-band top but as the band gap is opened, the acceptor state becomes more localized and the respective level is shifted upward. Taking polaronic effects into account, we place the ionization level of ${\text{Li}}_{\text{Zn}}$ between ${E}_{v}+0.60\text{ }\text{eV}$ and ${E}_{v}+1.1\text{ }\text{eV}$. This deeper level explains the difficulty in realizing $p$-type ZnO using Li as monodopant. Further, the mobility of the defects was investigated. While interstitial Li is mobile at low temperatures, independent of the stoichiometry, the diffusion of ${\text{Li}}_{\text{Zn}}$ depends on the concentrations of intrinsic defects. Our calculations show that in O-rich material, where the defect is more stable, the dominant diffusion process corresponds to a dissociative mechanism requiring a substantial activation energy.
Effects of the nonlinearly-induced timing and amplitude jitter on the performance of different modulation formats in WDM optical fiber communications systems (2004)

We compare the CRZ, RZ, and NRZ modulation formats in WDM systems. We identify the physical phenomena responsible for the performance advantages and disadvantages of these formats during transmission and in the receiver.
Figure S6 from Bortezomib Stabilizes and Activates p53 in Proliferative Compartments of Both Normal and Tumor Tissues <i>In Vivo</i> (2023)

&lt;p&gt;Upregulation of p53 target genes in response to irradiation in intestinal adenomas.&lt;/p&gt;
Passive mode-locking of acentric Yb-doped borate crystals (2010)

Passive mode locking of the self-frequency doubling Yb:YAB and Yb:YCOB crystals with a saturable absorber mirror is studied at the fundamental wavelength with diode pumping. All essential polarization orientations including the self-frequency doubling configuration are considered. For the first time sub-90 fs pulses at the fundamental are obtained with both materials and the improvement in terms of pulse length in comparison to previous work is about 2.3 times (Yb:YAB) and 2.8 times (Yb:YCOB). The Yb:YAB laser generates pulses as short as 87 fs at 1050 nm with a 0.4% output coupler. The shortest pulses (76 fs) are obtained with a Y-cut Yb:YCOB for E ∥ Z. With an output coupler of 0.4% the oscillation wavelength is 1056 nm. For E ∥ X the mode-locked Yb:YCOB laser operates on a different Stark transition (at longer wavelengths) than for E ∥ gg and E ∥ Z.
Departures from Many Queues in Series (1991)

We consider a series of $n$ single-server queues, each with unlimited waiting space and the first-in first-out service discipline. Initially, the system is empty; then $k$ customers are placed in the first queue. The service times of all the customers at all the queues are i.i.d. with a general distribution. We are interested in the time $D(k,n)$ required for all $k$ customers to complete service from all $n$ queues. In particular, we investigate the limiting behavior of $D(k,n)$ as $n \rightarrow \infty$ and/or $k \rightarrow \infty$. There is a duality implying that $D(k,n)$ is distributed the same as $D(n,k)$ so that results for large $n$ are equivalent to results for large $k$. A previous heavy-traffic limit theorem implies that $D(k,n)$ satisfies an invariance principle as $n \rightarrow \infty$, converging after normalization to a functional of $k$-dimensional Brownian motion. We use the subadditive ergodic theorem and a strong approximation to describe the limiting behavior of $D(k_n,n)$, where $k_n \rightarrow \infty$ as $n \rightarrow \infty$. The case of $k_n = \lbrack xn \rbrack$ corresponds to a hydrodynamic limit.
GTP-binding proteins may stimulate insulin biosynthesis in rat pancreatic islets by enhancing the signal-recognition-particle-dependent translocation of the insulin mRNA poly-/mono-some complex to the endoplasmic reticulum (1991)

We aimed to elucidate the putative role of GTP-binding proteins in the regulation of insulin biosynthesis. For this purpose, freshly isolated rat islets were incubated in the presence of liposomes containing GDP, guanosine 5'-[beta-thio]diphosphate (GDP[S]), GTP, guanosine 5'-[gamma-thio]triphosphate (GTP[S]), guanosine 5'-[beta gamma-methylene]triphosphate (p[CH2]ppG), guanosine 5'[beta gamma-imido]triphosphate (p[NH]ppG) and ATP, and the effects of the liposomal delivery of these substances on rates of biosynthesis of insulin and total protein were determined. Insulin biosynthesis during a 1 h incubation at 1.67 mM-glucose was stimulated by ATP- and GTP[S]-containing liposomes as compared with control liposomes. At 16.7 mM-glucose, only the GTP[S]-containing liposomes stimulated insulin biosynthesis. No inhibition of islet protein and insulin synthesis was observed with GDP-, GDP[S]-, p[CH2]ppG- and p[NH]ppG-containing liposomes. By determining the subcellular distribution of insulin mRNA, it was found that the mRNA content associated with microsomes was increased and that associated with the cytosolic mono-/poly-somes decreased when the islets were incubated with GTP[S]-containing liposomes, resulting in an approximate doubling of the ratio of microsomal to polysomal-associated insulin mRNA. ATP-containing liposomes produced no effects on the association of insulin mRNA with microsomes. By using photoaffinity labelling and immunoprecipitation techniques, specific binding of GTP[35S] to the alpha-subunit of the signal-recognition particle (SRP) receptor in islet homogenates containing physiological concentrations of GTP and GDP was demonstrated. These findings suggest that the GTP-binding subunit(s) of the SRP receptor, and possibly also of other GTP-binding proteins involved in this process, may regulate insulin biosynthesis by stimulating the translocation of insulin mRNA to the endoplasmic reticulum and by increasing preproinsulin-peptide translocation into the lumen of the reticulum.
Using a limited mapping strategy to identify major QTLs for resistance to grapevine powdery mildew (Erysiphe necator) and their use in marker-assisted breeding (2010)

A limited genetic mapping strategy based on simple sequence repeat (SSR) marker data was used with five grape populations segregating for powdery mildew (Erysiphe necator) resistance in an effort to develop genetic markers from multiple sources and enable the pyramiding of resistance loci. Three populations derived their resistance from Muscadinia rotundifolia 'Magnolia'. The first population (06708) had 97 progeny and was screened with 137 SSR markers from seven chromosomes (4, 7, 9, 12, 13, 15, and 18) that have been reported to be associated with powdery or downy mildew resistance. A genetic map was constructed using the pseudo-testcross strategy and QTL analysis was carried out. Only markers from chromosome 13 and 18 were mapped in the second (04327) and third (06712) populations, which had 47 and 80 progeny, respectively. Significant QTLs for powdery mildew resistance with overlapping genomic regions were identified for different tissue types (leaf, stem, rachis, and berry) on chromosome 18, which distinguishes the resistance in 'Magnolia' from that present in other accessions of M. rotundifolia and controlled by the Run1 gene on chromosome 12. The 'Magnolia' resistance locus was termed as Run2.1. Powdery mildew resistance was also mapped in a fourth population (08391), which had 255 progeny and resistance from M. rotundifolia 'Trayshed'. A locus accounting for 50% of the phenotypic variation mapped to chromosome 18 and was named Run2.2. This locus overlapped the region found in the 'Magnolia'-based populations, but the allele sizes of the flanking markers were different. 'Trayshed' and 'Magnolia' shared at least one allele for 68% of the tested markers, but alleles of the other 32% of the markers were not shared indicating that the two M. rotundifolia selections were very different. The last population, 08306 with 42 progeny, derived its resistance from a selection Vitis romanetii C166-043. Genetic mapping discovered a major powdery mildew resistance locus termed Ren4 on chromosome 18, which explained 70% of the phenotypic variation in the same region of chromosome 18 found in the two M. rotundifolia resistant accessions. The mapping results indicate that powdery mildew resistance genes from different backgrounds reside on chromosome 18, and that genetic markers can be used as a powerful tool to pyramid these loci and other powdery mildew resistance loci into a single line.