gebhart/patrepeval huggingface dataset:
Regulation of Aspartokinase Activity in Clostridium perfringens (1973)

Cells of Clostridium perfringens type D apparently possess only a single species of aspartokinase. This enzyme has been partially purified and shown to be feedback inhibited by meso-diaminopimelate in an allosteric manner. The inhibitor exerts its action noncompetitively with respect to both substrates. The kinetic analysis further indicates that no homotropic cooperative interactions occur between either multiple substrate or inhibitor sites. Like aspartokinases from other bacteria, the clostridial enzyme is stimulated by the presence of either potassium or ammonium cations. A molecular weight of 102,000 was estimated for the enzyme following gel-filtration chromatography. Enzyme activity remains relatively constant throughout the growth cycle of the organism even well into the stationary growth phase. These results are discussed in terms of the role of the enzyme in the growth of the organism.
Circularly symmetric phase filters for control of primary third-order aberrations: coma and astigmatism (2006)

A quartic phase retardation function is described that reduces the variation of the intensity of the focal point of incoherent imaging systems suffering from primary third-order aberrations limited to coma and astigmatism. Corresponding modulation transfer functions are shown to remain practically invariant for moderate amounts of coma and astigmatism.
3′,6′-Bis(diethylamino)-2-(quinolin-8-yl)spiro[1H-isoindoline-1,9′-xanthen]-3(2H)-one (2006)

The title compound, C37H36N4O2, has been obtained by the reaction of 2-[3,6-bis­(diethyl­amino)-9H-xanthen-9-yl]benzoyl chloride with 8-amino­quinoline. The mol­ecular packing in the crystal structure is stabilized by weak C—H⋯O hydrogen bonding.
Photometric determination of lead with chromopyrazole I (1986)

The influence of various factors on the complex formation of lead with iodide ions and chromopyrazole I in the presence of surfactants was studied, and the optimum conditions of complex formation were found. An influence of the nature of the surfactant was established, and the mechanism of the stabilization of the lead compounds formed was proposed. The formation of an ionic associate of lead with the general formula (PbI/sub 4/)R/sub 2/, where R is a singly charged cation of the dye, under optimum conditions was established using electronic and IR spectroscopy. The main spectrophotometric characteristics and the influence of extraneous ions on complex formation were studied. A highly sensitive and selective photometric method was developed for the determination of lead.
Short Communication In search of polymorphic Alu insertions with restricted geographic distributions (2007)

Alu elements are transposable elements that have reached over one million copies in the human genome. Some Alu elements inserted in the genome so recently that they are still polymorphic for insertion presence or absence in human populations. Recently, there has been an increasing interest in using Alu variation for studies of human population genetic structure and inference of individual geographic origin. Currently, this requires a high number of Alu loci. Here, we used a linker-mediated polymerase chain reaction method to preferentially identify low-frequency Alu elements in various human DNA samples with different geographic origins. The candidate Alu loci were subsequently genotyped in 18 worldwide human populations (∼370 individuals), resulting in the identification of two new Alu insertions restricted to populations of African ancestry. Our results suggest that it may ultimately become possible to correctly infer the geographic affiliation of unknown samples with high levels of confidence without having to genotype as many as 100 Alu loci. This is desirable if Alu insertion polymorphisms are to be used for human evolution studies or forensic applications.
Toward a table-top quantum computer (2000)

To investigate the scaling issues for quantum computation using thermalized ensembles of spins, we are building a rudimentary quantum computer using nuclear magnetic resonance (NMR). We discuss the experimental issues facing the scalability of this technique including the number of qubits, issues of classical versus quantum behavior, and polarization. Due to the weak signals measured in NMR, the most significant challenge to practical devices is polarization enhancement. We present the architecture for a simple NMR spectrometer that is table-top, low-cost, and software-radio driven using commodity electronics and unconventional permanent magnet designs. Optimizations specific to using this machine as a computational device, such as probe design, compilation, and on-line control, are discussed.
Machine Learning-based Cyber-Physical Anomaly Detection in Wide Area Voltage Control Systems (2022)

Wide-area voltage control systems (WAVCS) are widely deployed in power grid to improve the voltage stability in transmission system using Flexible AC Transmission System (FACTS) devices. The WAVCS relies on wide-area measurement and control signals for closed-loop control of FACTS devices to improve the transient voltage stability in power grid in real-time. Since the WAVCS utilizes a cyber-layer communication during its normal operation, they are susceptible to cyber attacks from adversaries which can lead to a voltage collapse if the attacks go undetected and unmitigated. This paper proposes a supervised machine learning (ML)-based anomaly detection algorithm for detecting various stealthy cyber attacks in the context of WAVCS cybersecurity. In particular, a fuzzy logic-based wide-area controller, as proposed by the Bonneville Power Administration (BPA), is implemented on the Kundur’s four machine two-area system that is integrated with a static var compensator (SVC) to improve voltage profile on sensitive buses. Later, different types of data integrity attacks, including pulse and ramp attacks, are considered on the wide-area measurement and control signals to analyze the performance of the proposed anomaly detector. Our experimental evaluation shows a promising performance with a high true-positive rate (more than 99%) and low false-negative rate (less than 1%) while exhibiting a small prediction time.
Louis Moulinier: Quelques hypothèses relatives à la géographie d'Homère dans l'Odyssée. (Publ. des Annales la Fac. des Lettres d'Aix-en-Provence, 23.) Pp. 132; 2 maps. Paris: Klincksieck, 1959. Paper, 14 fr. (1961)

Louis Moulinier: Quelques hypothèses relatives à la géographie d'Homère dans l'Odyssée. (Publ. des Annales la Fac. des Lettres d'Aix-en-Provence, 23.) Pp. 132; 2 maps. Paris: Klincksieck, 1959. Paper, 14 fr. - Volume 11 Issue 2
Effects of transducers on guided wave based structural health monitoring (2018)

Lead Zirconate Titanate (PZT) transducers are commonly used to generate guided acoustic waves for health monitoring of structures made of isotropic and anisotropic materials. Therefore, characterization of PZT transducers is very important not only for reliability but also for the accuracy of data. In this research, Scanning Acoustic Microscopy (in reflection mode) at 30 MHz excitation frequency is conducted for determining the quality of disc type guided wave transducers. Multiple samples are scanned but only four transducers are selected for further investigation. Guided waves are dispersive in nature and multiple guided wave modes can coexist at single frequency. It has been already reported in the literature that good bonding condition between transducer and testing material is very important to restrict other factors that can affect the strength of recorded signals. Chirp signal is excited in the specimen in a single sided excitation/detection setup to investigate the effects of transducer quality on monitoring plate like structures. Sideband peak count technique is used for comparative study of transducers.
Biological synthesis of l-ascorbic acid in animal tissues: conversion of d-glucuronolactone and l-gulonolactone into l-ascorbic acid (1960)

Research Article| August 01 1960 Biological synthesis of l-ascorbic acid in animal tissues: conversion of d-glucuronolactone and l-gulonolactone into l-ascorbic acid I. B. Chatterjee; I. B. Chatterjee 1Department of Applied Chemistry, Calcutta University, India Search for other works by this author on: This Site PubMed Google Scholar G. C. Chatterjee; G. C. Chatterjee 1Department of Applied Chemistry, Calcutta University, India Search for other works by this author on: This Site PubMed Google Scholar N. C. Ghosh; N. C. Ghosh 1Department of Applied Chemistry, Calcutta University, India Search for other works by this author on: This Site PubMed Google Scholar J. J. Ghosh; J. J. Ghosh 1Department of Applied Chemistry, Calcutta University, India Search for other works by this author on: This Site PubMed Google Scholar B. C. Guha B. C. Guha 1Department of Applied Chemistry, Calcutta University, India Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1960) 76 (2): 279–292. https://doi.org/10.1042/bj0760279 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation I. B. Chatterjee, G. C. Chatterjee, N. C. Ghosh, J. J. Ghosh, B. C. Guha; Biological synthesis of l-ascorbic acid in animal tissues: conversion of d-glucuronolactone and l-gulonolactone into l-ascorbic acid. Biochem J 1 August 1960; 76 (2): 279–292. doi: https://doi.org/10.1042/bj0760279 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search © 1960 CAMBRIDGE UNIVERSITY PRESS1960 Article PDF first page preview Close Modal You do not currently have access to this content.
Long-Term Secretion of Therapeutic Proteins from Genetically Modified Skeletal Muscles (1996)

Protein delivery from genetically modified skeletal muscle has been reported previously. However, a stable and prolonged secretion was obtained in immunocompromised or newborn animals only. To evaluate the clinical relevance of this approach, we have transduced myoblasts from an adult beta-glucuronidase-deficient (MPS VII) mouse with retroviral vectors carrying either the human beta-glucuronidase cDNA or the murine erythropoietin (Epo) cDNA. The cells were then grafted into the tibialis anterior muscle of adult immunocompetent MPS VII recipients. Protein expression was controlled either by ubiquitous or muscle-specific transcriptional regulatory elements. Animals were analyzed over an 8-month period. The in situ detection of beta-glucuronidase activity revealed up to 60% of genetically modified myofibers in the recipient muscles. The human desmin promoter and enhancer showed the highest in vivo expression. Secretion of beta-glucuronidase induced a disappearance of lysosomal storage lesions in the liver and spleen of recipient animals. Delivery of Epo led to a permanent increase of hematocrit values over 3 months. These results showed that the transplantation of genetically modified myoblasts allowed a sustained secretion of recombinant proteins at therapeutic levels in immunocompetent adult mice. They suggest that the approach may be considered for human applications.
Electron capture dissociation mass spectrometry of peptide cations containing a lysine homologue: a mobile proton model for explaining the observation of b‐type product ions (2006)

Eleven doubly protonated peptides with a residue homologous to lysine were investigated by electron capture dissociation mass spectrometry (ECD-MS). Lysine homologues provide the unique opportunity to examine the ECD fragmentation behavior by allowing us to vary the length of the lysine side chain, with minimal structural change. The lysine homologue has a primary amine side chain with a length that successively decreases by one methylene (CH(2)) unit from the --CH(2)CH(2)CH(2)CH(2)NH(2) of lysine and the accompanying decrease of its proton affinities: lysine (K), 1006.5(+/-7.2) kJ/mol; ornithine (K(*)), 1001.1(+/-6.6) kJ/mol; 2,4-diaminobutanoic acid (K(**)), 975.8(+/-7.4) kJ/mol; 2,3-diaminopropanoic acid (K(***)), 950.2(+/-7.2) kJ/mol. In general, the lysine-homologous peptides exhibited overall ECD fragmentation patterns similar to that of the lysine-containing peptides in terms of the locations, abundances, and ion types of products, such as yielding c(+) and z(+.) ions as the dominant product ions. However, a close inspection of product ion mass spectra showed that ECD-MS for the alanine-rich peptides with an ornithinyl or 2,4-diaminobutanoyl residue gave rise to b ions, while the lysinyl-residue-containing peptides did not, in most cases, produce any b ions. The peptide selectivity in the generation of b(+) ions could be understood from within the framework of the mobile proton model in ECD-MS, previously proposed by Cooper (Ref. 29). The exact mass analysis of the resultant b ions reveals that these b ions are not radical species but rather the cationic species with R-CO(+) structure (or protonated oxozalone ion), that is, b(+) ions. The absence of [M+2H](+.) species in the ECD mass spectra and the selective b(+)-ion formation are evidence that the peptides underwent H-atom loss upon electron capture, and then the resulting reduced species dissociated following typical MS/MS fragmentation pathways. This explanation was further supported by extensive b(+) ions generated in the ECD of alanine-based peptides with extended conformations.
Increasing Perceived Safety in Motion Planning for Human-Drone Interaction (2023)

Safety is crucial for autonomous drones to operate close to humans. Besides avoiding unwanted or harmful contact, people should also perceive the drone as safe. Existing safe motion planning approaches for autonomous robots, such as drones, have primarily focused on ensuring physical safety, e.g., by imposing constraints on motion planners. However, studies indicate that ensuring physical safety does not necessarily lead to perceived safety. Prior work in Human-Drone Interaction (HDI) shows that factors such as the drone's speed and distance to the human are important for perceived safety. Building on these works, we propose a parameterized control barrier function (CBF) that constrains the drone's maximum deceleration and minimum distance to the human and update its parameters on people's ratings of perceived safety. We describe an implementation and evaluation of our approach. Results of a within-subject user study (N=15) show that we can improve perceived safety of a drone by adjusting to people individually.
Introducing Amino Group on 1(2H)-Phthalazinone Derivatives (1981)

Nitration of 4-ethoxycarbonyl-l(2X)-phthalazinone (4) with KNO and conc sulfuric acid at room temperature afforded the 3 corresponding 5-nitro derivative (5) in about 50% yield.The latter -
J044031 Evaluation of environmental effect on delamination detection in CFRP laminates based on the mode conversions of broadband Lamb waves (2012)

The authors have developed a built-in ultrasonic propagation system consisting of macro fiber composite (MFC) actuators and fiber Bragg grating (FBG) sensors. This system can propagate multiple modes of broadband Lamb waves in CFRP laminates and can identify all the modes by synchronized actuation of two MFCs and measurement with two FBGs on both surfaces of the laminate. Hence, a new delamination detection method has been established on the basis of the change in the frequency dispersion caused by the mode conversions at the delamination edges. However, the frequency dispersion is also affected by the temperature change. From a practical point of view, it is important to compensate the temperature effect on this detection method. Hence, in this research, we conducted experiments for CFRP quasi-isotropic laminates with an artificial delamination at various temperatures in a thermostatic chamber, in order to evaluate the effect of environmental temperature on the frequency dispersion of anti-symmetric (A) modes. As a result, with an increase in the temperature, the time of flight (ToF) of A_0 mode increased and that of A_1 mode decreased. However, ToF of A_0 was independent of delamination ljpnth D and the dispersion slope of A_1 changed depending on the D because of the mode conversions. Hence the environmental temperature can be estimated from the ToF of A_0, and the estimated temperature can be used to compensate the temperature effect on the change in the dispersion slope of A_1. Therefore, we will be able to evaluate the delamination ljpnth in CFRP with compensation of temperature change. In addition, we found that change in mechanical properties of the CFRP by the temperature increase affected the mode dispersion from comparing the results between experiment and FEA.
Kinetic stabilisation of a modular protein by domain interactions (1998)

Protein S, a two-domain spore coat protein from Myxococcus xanthus, is structurally related to eye-lens Pr crystallins. No natural monomeric one-domain member of this protein superfamily is known. To determine the stability of the single domains and to explain the ubiquitous domain duplication, the isolated domains of protein S were constructed. The N-domain is thermodynamically more stable than the C-domain. In intact protein S, domain interactions lead to an apparent decrease in stability of the N-terminal domain, whereas the C-terminal domain is stabilised. In contrast, unfolding kinetics of both domains are decreased 100-fold due to interactions in the complete molecule.
Variability of Tissue Mechanical Response in Sus Domesticus Porcine Models from in vivo to ex vivo Conditions (2022)

Abstract Healthcare simulators have been demonstrated to be a valuable resource for training several technical and nontechnical skills. A gap in the fidelity of tissues has been acknowledged as a barrier to application for current simulators; especially for interventional procedures. Inaccurate or unrealistic mechanical response of a simulated tissue to a given surgical tool motion may result in negative training transfer and/or prevents the “suspension of disbelief” necessary for a trainee to engage in the activity. Thus, where it is relevant to training outcomes, there should be an effort to create healthcare simulators with simulated tissue mechanical responses that match or represent those of biological tissues. Historically, this data is most often gathered from preserved (post mortem) tissue; however, there is a concern that the mechanical properties of preserved tissue, that lacks blood flow, may lack adequate accuracy to provide the necessary training efficacy of simulators. This work explores the effect of the “state” of the tissue testing status on liver and peritoneal tissue by using a customized handheld grasper to measure the mechanical responses of representative porcine ( Sus domesticus ) tissues in N=5 animals across five test conditions: in vivo, postmortem (in-situ), ex vivo (immediately removed from fresh porcine cadaver), post-refrigeration, and post-freeze-thaw cycle spanning up to 72 hours after death. No statistically significant difference was observed in the mechanical responses due to grasping between in vivo and post-freeze conditions for porcine liver and peritoneum tissue samples (p = 0.05 for derived stiffness at grasping force values F = 5N and 6.5N). Furthermore, variance between in vivo and post-thaw conditions within each animal, was comparable to the variance of the in vivo condition between animals. Thus, the use of thawed, preserved tissue for the further study and emulation of mechanical perturbation of the liver and peritoneum can be considered.
Evaluation of a CCD camera system for BRDF retrieval for remote sensing applications for vicarious calibration (2000)

A CCD-camera based system for the retreival of bidirectional reflectance distribution function (BRDF) data has been evaluated for vicarious calibration applications. This evaluation is done by assessing the calibration requirements necessary to retrieve BRDF data for the improvement of the vicarious calibration approach, and then by examining the calibration problem itself. A sensitivity analysis shows that for a top of the atmosphere (TOA) radiance accurate to 0.1%, instrumental biases must be under 5% while pixel-to-pixel gain variations may be as great as 10%. A method for achieving the calibration requirements using a CCD-based BRDF camera system constructed by the Remote Sensing Group (RSG) at the University of Arizona Optical Sciences Center is presented. A relative calibration level of approximately 1% across the camera array is found to be achievable given the laboratory facilities of the RSG. Software designed to extract BRDF data from the BRDF camera system output and convert the data into a form usable in the RSG's radiative transfer code are described and demonstrated on example data sets. A diffuse-light correction algorithm and software to perform the correction on BRDF camera data are described, and the software is tested against several example data sets to evaluate the retrieval accuracy of the code. Retrieval accuracies of better than 0.5% in phase and better than 0.01% in radiance have been achieved with this code using modeled data and at a 45-degree solar zenith angle. Based on these results, CCD-camera based systems can be used to improve the level of accuracy of TOA radiance calculations for vicarious calibration.
Immunolocalization and distribution of proteoglycans in carious dentine (2015)

This study presents new insights into the involvement of PGs in the progression of caries.
Plans d'expériences pour modèles neuronaux (2004)

Poster ayant obtenu le Lambda-Mu d'Or du congrès Format du poster : 1,20 x 0,80
Ionic Liquid Gating Control of Spin Reorientation Transition and Switching of Perpendicular Magnetic Anisotropy (2018)

Electric field (E-field) modulation of perpendicular magnetic anisotropy (PMA) switching, in an energy-efficient manner, is of great potential to realize magnetoelectric (ME) memories and other ME devices. Voltage control of the spin-reorientation transition (SRT) that allows the magnetic moment rotating between the out-of-plane and the in-plane direction is thereby crucial. In this work, a remarkable magnetic anisotropy field change up to 1572 Oe is achieved under a small operation voltage of 4 V through ionic liquid (IL) gating control of SRT in Au/[DEME]<sup>+</sup> [TFSI]<sup>-</sup> /Pt/(Co/Pt)<sub>2</sub> /Ta capacitor heterostructures at room temperature, corresponding to a large ME coefficient of 378 Oe V<sup>-1</sup> . As revealed by both ferromagnetic resonance measurements and magnetic domain evolution observation, the magnetization can be switched stably and reversibly between the out-of-plane and in-plane directions via IL gating. The key mechanism, revealed by the first-principles calculation, is that the IL gating process influences the interfacial spin-orbital coupling as well as net Rashba magnetic field between the Co and Pt layers, resulting in the modulation of the SRT and in-plane/out-of-plane magnetization switching. This work demonstrates a unique IL-gated PMA with large ME tunability and paves a way toward IL gating spintronic/electronic devices such as voltage tunable PMA memories.
ChemInform Abstract: A HYBRID BIRCH‐CLAISEN METHODOLOGY FOR ARYLATION AT ALLYLIC TERMINUSES: SYNTHESIS OF (.+‐.)‐HERBERTENE (1983)

Abstract Aus den Benzoesäuren (I) erhält man durch Birch‐Reduktion, Veresterung mit Dimethylallylalkohol (II), Umlagerung und nachfolgende oxidative Decarboxylierung die substituierten Aromaten (III).