gebhart/patrepeval huggingface dataset:
Isolation and characterization of rat liver free and membrane-bound polysomal messenger ribonucleoprotein particles (1977)

Rat liver mRNA-labeled free and membrane-bound polysomes uncontaminated with nuclear or cytoplasmic ribonucleoprotein particles were dissociated with EDTA and the released messenger ribonucleoprotein particles isolated using an oligo(dT)-cellulose column. Seventy percent of the labeled mRNA applied bound to the column. Binding of mRNP was dependent on the presence of a poly(A) segment in the RNA. The nonbound material contained most of the ribosomal subunits and also messenger-sized poly(A-)RNA molecules associated with protein. The free mRNP fraction bound to the column, washed with 250 mM NaCl, and subsequently eluted contained seven proteins ranging in molecular weight from 52 000 to 138 000 only one of which was found in the fraction not bound to the column. Furthermore, these proteins were shown to have a higher affinity for poly(A+)RNA as compared with rRNA. The membrane-bound mRNP contained five proteins, four of which were identical with those associated with free mRNP. Membrane-bound mRNP were disrupted at a lower salt concentration than the free. The proteins found associated with free and membrane-bound polysomal mRNP appeared to be clustered in the poly(A) region of the molecule. The implications of these findings are discussed.
A pediatric phase I trial and pharmacokinetic study of MLN8237, an oral selective small molecule inhibitor of aurora a kinase: A Children's Oncology Group Phase I Consortium study. (2010)

9529 Background: MLN8237, a selective small molecule inhibitor of Aurora A Kinase, has activity in a broad range of in vitro and in vivo preclinical models of pediatric cancer. We performed a phase I trial to determine the dose limiting toxicities (DLT), maximum tolerated dose (MTD), and pharmacokinetics (PK) of MLN8237 in children with refractory solid tumors. Methods: MLN8237 was administered orally either once daily (part A1) or divided twice daily (part A2) for 7 days, every 21 days. Using the rolling-six design, four dose levels (45, 60, 80, 100 mg/m2/d) were evaluated on the once daily regimen, and subsequently two dose levels (60 and 80 mg/m2/d) on the twice-daily regimen. Pharmacokinetic studies were performed with the initial dose, with trough drug concentrations also being obtained at steady-state. Results: 37 patients were enrolled, 32 were fully evaluable for toxicity [median (range) age 12.5 yrs (4.8 - 21.6)]: 21 on part A1 and 11 on part A2. In part A1, 1/6 pts developed DLT (grade 3 mucositis) at 45 mg/m2 and 1/6 developed DLT (grade 4 mood alteration) at 80 mg/m2. At 100 mg/m2, 3/4 patients developed DLT (neutropenia –thrombocytopenia), thus exceeding the MTD. In part A2, 2/6 patients developed dose limiting myelosuppression at 80 mg/m2; one of whom also experienced grade 3 mucositis. 1/5 patient experienced DLT (grade 3 alkaline phosphatase) at 60 mg/m2. Of note, 5/11 patients experienced hand-foot-skin syndrome (grade 1 - 3) on the bid schedule versus 1/21 (grade 2) on qd schedule. A high degree of inter- patient variability in systemic exposure was observed. Average trough concentrations exceeded 1 μ M, the efficacious concentration in preclinical studies. The mean (±SD) steady state MLN8237 trough concentrations at the 45, 60, 80 and 100 mg/m2/d dose levels were 5.1 ± 5.7, 4.9 ± 6.3, 3.7 ± 3.8, and 5.5 ± 4.9 μ M, respectively. Conclusions: Although the divided dose regimen appears to be well tolerated in adults, children experienced more myelosuppression and hand-foot-skin syndrome with divided versus once daily dosing. Therefore the recommended pediatric phase 2 dose and schedule of MLN8237 is 80 mg/m2/d administered once daily for 7 days. No significant financial relationships to disclose.
Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells (2014)

The function of many eukaryotic proteins is regulated by highly dynamic changes in their nucleocytoplasmic distribution. The ability to precisely and reversibly control nuclear translocation would, therefore, allow dissecting and engineering cellular networks. Here we develop a genetically encoded, light-inducible nuclear localization signal (LINuS) based on the LOV2 domain of Avena sativa phototropin 1. LINuS is a small, versatile tag, customizable for different proteins and cell types. LINuS-mediated nuclear import is fast and reversible, and can be tuned at different levels, for instance, by introducing mutations that alter AsLOV2 domain photo-caging properties or by selecting nuclear localization signals (NLSs) of various strengths. We demonstrate the utility of LINuS in mammalian cells by controlling gene expression and entry into mitosis with blue light.
Pharmacokinetics of cefetamet in plasma and skin blister fluid (1996)

Cefetamet pivoxil is an oral cephalosporin with enhanced affinity for the target penicillin-binding proteins 1 and 3 and an increased stability to beta-lactamases compared with older cephalosporins, such as cefalexin or cefaclor. The pharmacokinetics of cefetamet pivoxil was determined after the seventh and final dose of 500 mg of cefetamet pivoxil in eight healthy volunteers. Concentrations in plasma and cantharidin-induced skin blister fluid were determined by a high-performance liquid chromatography method. In addition, protein binding was assessed. Cmax was 4.8 +/- 1.7 micrograms/ml in skin blister fluid and 5.1 +/- 2.1 micrograms/ml in plasma. Tmax was delayed in skin blister fluid compared with plasma (3.9 +/- 1 versus 2.8 +/- 0.8 h; P < 0.001), and t1/2 was longer in skin blister fluid than in plasma (3.1 +/- 0.5 versus 2.3 +/- 0.3; P < 0.005). The mean percent penetration into cantharide blister fluid was 129% +/- 24% when measured as total drug and 149% +/- 28% when measured as free drug (P < 0.001). These data suggest that cefetamet has an excellent penetration into inflammatory interstitial fluid.
Loaded Dendritic Cells as Melanoma Vaccines (1998)

Dendritic cells (DCs) are professional antigen-presenting cells that can induce peptide-specific responses by cytotoxic T lymphocytes (CTLs). As motile
<title>Investigation of various synthetic polymer scaffolds for enhanced mechanical strength during laser tissue soldering</title> (2002)

An investigation was conducted to assess the feasibility ofusing various synthetic polymers as scaffolds to traditional albumin protein solders with the aim of enhancing the mechanical strength of repairs formed during laser tissue soldering. Biodegradable polymer membranes of controlled porosity were fabricated with either polylactic acid (PLA), polyglycolic acid (PGA), or two different poly(L-lactic-co-glycolic acid) (PLGA) blend ratios, using a solvent-casting and particulate-leaching technique. In addition, membranes were prepared by combining each of the above mentioned polymers with poly(ethylene glycol) (PEG). The porous membranes provided a scaffold into which the traditional protein solder composition of serum albumin and indocyanine green dye mixed in deionized water was readily absorbed. Studies were conducted to assess the influence of various processing parameters on the strength of tissue repairs formed using the new adhesives. These parameters included the polymer type, the polymer/PEG blend ratio, the salt particle size, and the initial albumin weight fraction. No significant difference was observed between the four polymers investigated, however, variation of the polymer type and blend ratio is known to influence the degradation rate of the membranes. The addition of PEG to the films during the casting stage was observed to increase the flexibility of the membranes. Finally, repair strength increased significantly with an increase in the size ofpores present in the membranes and with increased protein concentration.
Near Infrared Diffusive Light Imaging with Ultrasound Localization (2001)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Quing Zhu, Nan Guang Guang Chen, Puyun Guo, Shikui Yan, and Daqing Piao, "Near Infrared Diffusive Light Imaging with Ultrasound Localization," Optics & Photonics News 12(12), 30-30 (2001) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Study on Asymmetric Tapered Slotline Antenna (2006)

Two novel but simple structures of asymmetric tapered slotline antenna (TSA) with synthesized radiation pattern is proposed. By adjusting the profiles of asymmetric conducting plates respectively, cutting the corrugated slots on side-edges, and setting a backed plate for single-TSA structure, the synthesized pattern in vertical plane can meet the requirement of specific application. The prototypes are considered as the scaled sample at 10 GHz for base-station of mobile communication system. A single-TSA sample is designed, its measured vertical pattern has 4deg beam tilting, null filling in the lower part, and suppressed side lobe (-19.9 dB) in the upper part; its HPBW in horizontal pattern is 65deg; it has 11.7 dBi of directive gain, and 3:1 frequency coverage (5.56~16.8 GHz) for VSWRles2:1. Another twin-TSA sample consists of a pair of asymmetric TSAs backed each other with a common excitation by a microstrip line, its simulated vertical pattern at 10 GHz has 7deg beam tilting in both sides, null filling in the lower part and suppressed side-lobe in the upper part; 34deg HPBW in vertical pattern and 60deg in horizontal pattern; and 8.4 dBi of directivity for each sides
Magnetic properties of gamma-Fe2O3 nanoparticles patterned inside SBA-15 silicas : evidence of surface effects (2006)

Poster présenté par Vincent Dupuis
Safety and Efficacy of Ubrogepant in Participants With Moderate to High Cardiovascular Risk (107) (2020)

To evaluate the safety of ubrogepant based on participants’ cardiovascular (CV) risk factors in the ACHIEVE I and ACHIEVE II phase 3 trials.
A low power and high speed Viterbi decoder chip for WLAN applications (2004)

This paper presents a 166Mb/s, 64-state, radix-4, 16-level soft decision Viterbi decoder for high speed WLAN applications. With the path merging and trace forward techniques, the memory read operations are reduced to save power consumption. A test chip is fabricated in 0.35/spl mu/m IP4M CMOS process, and can achieve the maximum throughput rate of 166Mbit/s under 3.3V. The measured power consumption is below 55mW under 166Mb/s throughput rate at 2.2V.
Soft-switched three-level capacitor clamping inverter with clamping voltage stabilization (2000)

This paper proposes a zero-voltage-switching scheme for the three-level capacitor clamping inverter. The proposed small-rating auxiliary circuit ensures not only zero-voltage switching of the main switches and zero-current switching of the auxiliary switches, but the clamping capacitor voltage of the inverter is also stabilized. The scheme prevents any voltage or current spikes from happening over the main or auxiliary switches and no modulation constraints are incurred. Operation, analysis, designing, and testing aspects of the scheme are detailed.
Tufted hair folliculitis: response to topical therapy with nadifloxacin. (1999)

Tufted hair folliculitis (THF) is a relatively rare disorder. We report two typical cases of THF, which are the first cases from Japan reported in the international literature and we discuss a new effective treatment for this condition.
Comparison of Clinical Efficacy of Norfloxacin (NFLX) and Pipemidic Acid (PPA) in the Treatment of Infectious Enteritis by a Double-Blind Method (1987)

The effect of norfloxacin (NFLX), a new quinolone agent, was compared with that of pipemidic acid (PPA) on infectious enteritis (bacillary dysentery, Campylobacter enteritis and enteropathogenic E. coli enteritis etc.) by a double-blind method.NFLX was administrated orally in a dose of 600mg/day, and PPA was administrated orally, 2, 000mg/day.The duration of the treatment was five days.Of 227 cases studied, 68 cases were excluded from analysis of effectiveness.The effectiveness was evaluated in 159 cases; 80 reveived NFLX and 79 reveived PPA.There was no statistical significance beteeen NFLX group and PPA group in terms of the background characteristics and MIC distribution for each drug.The results obtained were as follow:1. NFLX group was as same as PPA group in clinical effect judged by docters in charge.There was no significnat difference between both groups in defeverscence, disappearance of blood stool, decrease in number of defecation and improvement of stool character.2. In bacillary dysentery, the bacteriological effect of NFLX (100%) was superior to that of PPA (88.9%).Days required for eradication of organisms for NFLX group were shorter than those for PPA group with significant difference in Shigella spp.3. No subjective side effect was observed in both groups.Slightly abnormal laboratory findings were seen in 11 cases of NFLX group and 7 cases of PPA group.4. On clinical usefulness judged by the docters the ratio of usefulness were 100% in NFLX group and 86.0% in PPA group with significans difference in bacillary dysentery.From these results, NFLX is considered to be very useful medicine in the treatment of patients with infectious enteritis or carriers.
An Overview of MIL-STD-704, MIL-HDBK-704, 28 Volt DC Aircraft Utilization Compliance Testing, Electrical Power Quality Causes and Impacts (2004)

&lt;div class="htmlview paragraph"&gt;Aircraft electrical power quality effects the operation and life of avionics equipment. The military interface document that defines aircraft electrical power at the input to utilization equipment is MIL-STD-704. Testing is required to demonstrate compatibility of avionics with aircraft electrical power. This paper summarizes the aircraft electrical operating conditions and power groups of MIL-STD-704. The paper also gives an overview of the test guidance for 28 volt DC aircraft utilization equipment provided in the recently published handbook MIL-HDBK-704, “Guidance for Test Procedures for Demonstration of Utilization Equipment Compliance to Aircraft Electrical Power Characteristics”. Common causes of power quality parameters and the effects on avionics are given.&lt;/div&gt;
Custom wavefront optimization of intraocular lens asphericity (2008)

Abstract Purpose To investigate the optimal amount of ocular spherical aberration (SA) in an intraocular lens (IOL) to maximize optical quality. Methods In 54 eyes of 27 patients, implantation of aspheric IOLs was simulated with different amounts of SA to produce residual ocular SA from ‐0.30 microm to +0.30 microm. Corneal wavefront aberrations up to 6th order were computed from corneal topographic elevation data. HOAs were measured with the Imagine Eyes IRX3 wavefront aberrometer. The PSF was calculated for the residual ocular higher‐order aberrations (up to the 6th order)with fully corrected 2nd‐order aberrations. 14 patients were implanted with the Tecnis (AMO)IOL and 13 with the SN60WF (Alcon France). Results The amount of optimal SA could be predicted based on other HOAs of the cornea. The amount of ocular SA varied among subjects and could be predicted based on corneal HOAs. Conclusion Selection of an aspheric IOL should be performed according to corneal HOAs.
Modification of the optical and electrical properties of quantum dot infrared photodetector (QDIP) by thermal treatment (2005)

In this study, we have studied the thermal treatment effect not only on the optical and structural properties of QDIP structure but also device performance of the QDIP. The thermal treatment of InAs/GaAs QDIP structure have been carried out at the temperature range from 650<sup>o</sup>C and 850<sup>o</sup>C with SiO<sub>2</sub> capping layer for 1 minute under the N<sub>2</sub>-gas ambient. After the thermal treatment, the structure was processed to QDIP and its device characteristics such as dark current and IR photo-response were measured. Results show that the photoluminescence (PL) peak was blue-shifted from 1288nm to 1167nm while the peak of photo-currents spectrum was red-shifted from 7.6 um to 7.8 um after the thermal treatment. It is also noted that the thermally treated sample showed the increase of photo-currents, which resulted in the increase of detectivity.
A γ-Glutamyl Peptide Isolated from Onion (Allium cepaL.) by Bioassay-Guided Fractionation Inhibits Resorption Activity of Osteoclasts (2005)

One gram of onion added to the food of rats inhibits significantly (p < 0.05) bone resorption as assessed by the urinary excretion of tritium released from bone of 9-week-old rats prelabeled with tritiated tetracycline from weeks 1 to 6. To isolate and identify the bone resorption inhibiting compound from onion, onion powder was extracted and the extract fractionated by column chromatography and medium-pressure liquid chromatography. A single active peak was finally obtained by semipreparative high-performance liquid chromatography. The biological activity of the various fractions was tested in vitro on the activity of osteoclasts to form resorption pits on a mineralized substrate. Medium, containing the various fractions or the pure compound, was added to osteoclasts of new-born rats settled on ivory slices. After 24 h of incubation, the tartrate-resistant acid phosphatase positive multinucleated cells, that is, osteoclasts, were counted. Subsequently, the number of resorption pits was determined. Activity was calculated as the ratio of resorption pits/osteoclasts and was compared to a negative control, that is, medium containing 10% fetal bovine serum only and to calcitonin (10(-12) M) as a positive control. Finally, a single peak inhibited osteoclast activity significantly (p < 0.05). The structure of this compound was elucidated with high-performance liquid chromatography-electrospray ionization-mass spectrometry, time-of-flight electrospray ionization mass spectrometry, and nuclear magnetic resonance spectroscopy. The single peak was identified as gamma-L-glutamyl-trans-S-1-propenyl-L-cysteine sulfoxide (GPCS). It has a molecular mass of 306 Da and inhibits dose-dependently the resorption activity of osteoclasts, the minimal effective dose being approximately 2 mM. As no other peak displayed inhibitory activity, it likely is responsible for the effect of onion on bone resorption.
Phase Segregation of PEDOT:PSS on Textile to Produce Materials of >10 A mm−2 Current Carrying Capacity (2016)

Conductive textiles with exceptional electrical properties have been prepared by coating the conjugated polymer, poly(3,4‐ethylenedioxyphiophene)‐polystyrenesulfonate(PEDOT‐PSS), on polyethylene terephthalate (PET) nonwoven fabrics. Phase segregation from covalent bond formation to surface silica particles generates PEDOT‐PSS coated textiles that hold potential for wearable electronics due to the breathability of the fabric, low toxicity, easy processing and lightweight with high current carrying capacity. The conductive textiles were demonstrated for applications such as electrical connections and resistive heating. image
The invariant chain inhibits presentation of endogenous antigens by a human fibroblast cell line (1994)

The human fibroblast cell line, M1, expressing the products of transfected DRA and DRB1*0101 genes (M1-DR1) was unable to present intact influenza antigens to a series of DR1-restricted human T cell lines and clones, but was fully able to present synthetic peptides for T cell recognition. In contrast, M1-DR1 cells infected with live influenza virus were recognized by two polyclonal hemagglutinin- or whole virus-specific T cell lines and one of four T cell clones. This difference could not be accounted for simply by the ability of infectious virus to overcome a defect in antigen uptake by the M1-DR1 cells, in that direct studies of endocytosis showed that the M1 cells were more efficient than human B cells in the internalization of exogenous protein. These data suggested that the M1 cells were unable to present exogenous antigens but were capable of loading major histocompatibility complex (MHC) class II molecules with peptides derived from endogenous antigens. To investigate this further, the M1-DR1 cells were super-transfected with a cDNA encoding the p33 and p35 forms of the human invariant chain (Ii). Expression of the Ii chain was detected by intracytoplasmic staining of transfectants, and by metabolic labeling. Equimolar amounts of the p33 and p35 forms were detected, and the high level of p35 Ii was reflected by extensive retention of Ii protein in the endoplasmic reticulum. Addition of the Ii chain led to no recovery of presentation of intact antigens with DR1, but inhibited the presentation of live virus. These data indicate that MHC class II molecules in the M1-DR1 cells can be loaded with peptides derived from endogenous proteins, possibly in the biosynthetic pathway, and that the Ii chain has a role in limiting this route of class II antigen presentation.
Observation of thermal noise in a dynamically biased fiber-optic gyro (1996)

The effect of phase noise caused by thermal f luctuations in optical fiber is observed experimentally in a dynamically biased open-loop fiber-optic gyro. When source noise subtraction is used, thermal noise effects are readily observable. Total noise levels are in good agreement with theory. Contributions from shot, thermal, and electronic noise to the random walk coefficient are calculated, and the total is compared with experiment.
Wideband audio compression using a combined wavelet and WLPC representation (2002)

In this paper, we present results for a combined wavelet warped linear prediction (WLP) audio coder. In contrast to conventional LP, WLP allows for the control of frequency resolution to closely match the response of the human auditory system. The coder first uses WLP analysis on each frame of audio, and then applies a discrete wavelet transform (DWT) to the residual signal (prediction error). A psychoacoustic model is used in parallel to obtain a global masking threshold used in bit allocation. Bits are dynamically allocated to the DWT coefficients in an attempt to minimise the perceptually significant quantisation error. For monophonic signals sampled at 44.1 kHz, the coder achieves near transparent quality for a variety of speech and music signals at an average bit-rate of 64 kb/s. The power of the proposed coder resides in its easy scalability to lower bit rates.
Low Temperature Casting of Graphene with High Compressive Strength (Adv. Mater. 37/2012) (2012)

On page 5124, Rodney S. Ruoff, Litao Sun, and co-workers report a pH-mediated hydrothermal reduction and moulding of graphene oxide to realize high density (up to 1.6 g/cm3), compact 3-D graphene macrostructures with desired shapes. The present structures have a compressive strength of 361 MPa (6 times higher than conventional graphite products). This technique may contribute new products for, e.g., the graphite and other industries
Properties of porous HSQ-based films capped by plasma enhanced chemical vapor deposition dielectric layers (2002)

This article presents a study on Dow Corning® XLK™, an inorganic porous material with about 50% porosity and a dielectric constant of 2.0. It focuses on matters linked to sealing the porous film by depositing a plasma enhanced chemical vapor deposition (PECVD) dielectric cap layer. The study shows that the material can be modified during cap deposition due to the fast diffusion of reactants and radicals through the porous network, and acquire totally new properties which can be either beneficial or detrimental, depending on the chosen process. In particular, it is found that cap deposition processes on XLK in an oxidizing ambient, as used for SiO2 deposition, should be avoided. On the other hand, a beneficial modification of the dielectric film has been observed after SiC:H capping. It is also shown that there exists a critical thickness of capping material below which the cap layer reveals the presence of pinholes. The critical thickness value for a PECVD SiC:H cap layer on top of an XLK film is around 25 nm.