gebhart/patrepeval huggingface dataset:
The Immunocytochemical Detection of P53 Protein In Cytological Specimens: Technical Considerations (1994)

The p53 gene encodes a 393 amino acid nuclear phosphoprotein that appears to act as a cell cycle checkpoint, possibly by transactivating other target genes. Abnormalities of the p53 gene are common in a wide range of human tumours and are associated in many cases with immunologically detectable p53 protein. Detection of p53 immunoreactivity is uncommon in normal cells, but is frequently seen in neoplasia. Here we define the optimum conditions for the detection of p53 immunoreactivity in cytological material, including fixation and storage. Immersion in acetone-methanol for 10 min is optimal, and after air drying, smears or cytospin preparations can be stored at -70 degrees C for at least 6 months. We describe the range of controls necessary, including the use of positive control cell lines with known mutations of the p53 gene and defined abnormalities of p53 protein. Negative controls should include cell lines (or strains) with no p53 abnormality as well as the conventional negative immunological controls. It is only with these technical caveats and controls that p53 immunoreactivity can be performed reliably on cytological specimens.
Experimental augmentation of cerebral blood flow by intermittent aortic occlusion (1972)

✓ Intermittent occlusion of the descending aorta just below the origin of the brachiocephalic vessels by a preformed balloon passed via the femoral artery is capable of significantly increasing the pressure and flow in the common carotid artery. Regional cerebral blood flow determination by the krypton-85 washout technique measured maximum increases of over 40% of the controls, which could easily be achieved and maintained. This technique apparently takes advantage of the finite delay in autoregulatory response to the increased arterial pressure before the onset of maximal autoregulation. Dogs were “pumped” in this way for up to 18 hours and survived in good health. Principal problems with this technique were the development of cerebral edema in the presence of diffuse established cerebral anoxia, and a shock-like cardiovascular response if the intermittent aortic occlusion was discontinued too abruptly. The clinical application of this technique to cerebral ischemia secondary to postoperative vasospasm may not require the extremes of hyperperfusion used in these experiments.
Edge detection using the linear model (1986)

An edge detector based on the linear model is developed which utilizes the generalized likelihood ratio for statistical hypothesis testing. The detector is invariant to multiplicative changes in the gray-scale values of the image. Hence, thresholding based histogram segmentation is not required. The performance of this detector is analytically and experimentally compared to that of a gradient operator (Sobel) and is shown to have only a slightly poorer detection rate for a given false alarm rate.
Aztreonam in the management of Gram-negative infections in penicillin-allergic patients (1989)

Penicillins and cephalosporins, which are well-tolerated by most patients, can result in hypersensitivity reactions. This factor not only limits the use of beta-lactam antibiotics but also presents challenges to the selection of alternative drugs because of the potential for cross-reactivity. Evidence suggests that aztreonam, the prototype of the new monobactam class of beta-lactam antibiotics, may be a safe alternative antibiotic for managing Gram-negative infections in penicillin-allergic patients.
Weighted sum rate maximization on two user Gaussian interference channels with rate splitting (2009)

In interference limited networks treating interference as noise leads to performance degradation at high transmit powers. This can be avoided with rate splitting schemes (Han-Kobayashi region) where portions of the interference are decoded and removed at each receiver. We propose an algorithmic framework for solving the weighted sum rate maximization problem for the two user case of Gaussian interference channels. The polyblock outer approximation algorithm is used to solve the problem despite its nonconvexity. Contrary to usual brute force sampling approaches this framework may be extended to more users and other Gaussian interference models such as the X channel.
Subpellicular microtubules associate with an intramembranous particle lattice in the protozoan parasite Toxoplasma gondii (1997)

Application of Fourier analysis techniques to images of isolated, frozen-hydrated subpellicular microtubules from the protozoan parasite Toxoplasma gondii demonstrates a distinctive 32 nm periodicity along the length of the microtubules. A 32 nm longitudinal repeat is also observed in the double rows of intramembranous particles seen in freeze-fracture images of the parasite's pellicle; these rows are thought to overlie the subpellicular microtubules. Remarkably, the 32 nm intramembranous particle periodicity is carried over laterally to the single rows of particles that lie between the microtubule-associated double rows. This creates a two-dimensional particle lattice, with the second dimension at an angle of approximately 75 degrees to the longitudinal rows (depending on position along the length of the parasite). Drugs that disrupt known cytoskeletal components fail to destroy the integrity of the particle lattice. This intramembranous particle organization suggests the existence of multiple cytoskeletal filaments of unknown identity. Filaments associated with the particle lattice provide a possible mechanism for motility and shape change in Toxoplasma: distortion of the lattice may mediate the twirling motility seen upon host-cell lysis, and morphological changes observed during invasion.
Appendix B: Simulation of Quality Elements (2006)

This appendix contains sections titled: PSTN/ISDN Mobile Networks VoIP Wideband Transmission User Interfaces Test Rooms Simulation of Quality Elements: Summary
Controlling Perceptual Factors in Neural Style Transfer (2017)

Neural Style Transfer has shown very exciting results enabling new forms of image manipulation. Here we extend the existing method to introduce control over spatial location, colour information and across spatial scale. We demonstrate how this enhances the method by allowing high-resolution controlled stylisation and helps to alleviate common failure cases such as applying ground textures to sky regions. Furthermore, by decomposing style into these perceptual factors we enable the combination of style information from multiple sources to generate new, perceptually appealing styles from existing ones. We also describe how these methods can be used to more efficiently produce large size, high-quality stylisation. Finally we show how the introduced control measures can be applied in recent methods for Fast Neural Style Transfer.
Retinopathy (2003)

Blindness is one of the most feared complications of diabetes but also one of the most preventable. Diabetes is the commonest cause of blindness in people aged 30 to 69 years. Twenty years after the onset of diabetes, almost all patients with type 1 diabetes and over 60% of patients with type 2 diabetes will have some degree of retinopathy. Even at the time of diagnosis of type 2 diabetes, about a quarter of patients have established background retinopathy. Treatment can now prevent blindness in the majority of cases, so it is essential to identify patients with retinopathy before their vision is affected.
Quantitative mass spectrometric analysis of glycoproteins combined with enrichment methods (2014)

Mass spectrometry (MS) has been a core technology for high sensitive and high-throughput analysis of the enriched glycoproteome in aspects of quantitative assays as well as qualitative profiling of glycoproteins. Because it has been widely recognized that aberrant glycosylation in a glycoprotein may involve in progression of a certain disease, the development of efficient analysis tool for the aberrant glycoproteins is very important for deep understanding about pathological function of the glycoprotein and new biomarker development. This review first describes the protein glycosylation-targeting enrichment technologies mainly employing solid-phase extraction methods such as hydrizide-capturing, lectin-specific capturing, and affinity separation techniques based on porous graphitized carbon, hydrophilic interaction chromatography, or immobilized boronic acid. Second, MS-based quantitative analysis strategies coupled with the protein glycosylation-targeting enrichment technologies, by using a label-free MS, stable isotope-labeling, or targeted multiple reaction monitoring (MRM) MS, are summarized with recent published studies.
Oblivious cocktail watermarking by sparse code shrinkage: a regional- and global-based scheme (2002)

Watermarking with oblivious detection and high robustness capabilities together is still a challenging problem up to now. The existing methods are either robust or oblivious but it is difficult to achieve both goals simultaneously. In this paper, oblivious detection is formulated as a blind source separation problem by regarding the hidden watermarks as noise. To keep high robustness, our non-oblivious cocktail watermarking scheme, which is very robust, is adopted to combine with the oblivious detection mechanism. The proposed oblivious cocktail watermarking can be applied to global watermarking and regional watermarking. Experimental results have demonstrated the powerfulness of our method.
Efficient Infection of Citrus Plants with Different Cloned Constructs of Citrus tristeza virus Amplified in Nicotiana benthamiana Protoplasts (2005)

Infection of citrus is a critical but difficult step for the evaluation of cloned cDNA constructs of Citrus tristeza virus (CTV). So far, all attempts to directly infect citrus using either in vitro transcripts of CTV cDNA constructs or virus progeny extracted from transcript-inoculated Nicotiana benthamiana protoplasts have not been successful. We observed that RNA transcripts infect only 0.1% or less of N. benthamiana protoplasts, while virions can infect 80% or more of individual protoplasts. A multi-step procedure was devised to amplify the initial low-level infection in protoplasts to obtain quantities of virus sufficient for infection of citrus plants. Virions were extracted from transcript-inoculated N. benthamiana protoplasts and used for 4 to 10 sequential protoplast passages to maximize virus titer. Increased amplification levels were monitored by Northern blot analysis and ELISA. Extracts from the final protoplast passages were further concentrated by centrifuging through sucrose cushions, and the virus-containing zones were harvested and used for stem inoculation of citrus plants. We have successfully infected citrus plants with 38 constructs of CTV with this procedure, and plant infection rates frequently approach 100%.
Extended linear prediction tools for lossless audio coding (2004)

Two extension tools for enhancing the compression performance of prediction-based lossless audio coding are proposed. One is progressive-order prediction of the starting samples at the random access points, where the information of previous samples is not available. The first sample is coded as is, the second is predicted by first-order prediction, the third is predicted by second-order prediction, and so on. This can be efficiently carried out with PAR-COR (PARtial autoCORrelation) coefficients. The second tool is interchannel joint coding. Both predictive coefficients and prediction error signals are efficiently coded by interchannel differential or three-tap adaptive prediction. These new prediction tools lead to a steady reduction in bit rate when random access is activated and the interchannel correlation is strong.
A clear and present danger: tick-borne diseases in Europe (2009)

Ticks can transmit a variety of viruses, bacteria or parasites that can cause serious infections or conditions in humans and animals. While tick-borne diseases are becoming an increasing and serious problem in Europe, tick-borne diseases are also responsible for major depressions in livestock production and mortality in sub-Saharan Africa, Latin America and Asia. This review will focus on the most important circulating tick-transmitted pathogens in Europe (Borrelia spp., Anaplasma phagocytophilum, Babesia spp., tick-borne encephalitis virus, Rickettsia spp. and Crimean-Congo hemorrhagic fever virus).
Solving production process challenges with wafer-level reliability techniques (1995)

Many major semiconductor fabs are beginning to implement wafer-level reliability (WLR) as a means to improve both quality and yield of their product. Presently available WLR test structures provide a method for the reliability engineer to monitor the reliability of advanced state- of-the-art devices manufactured from CMOS, Bipolar, and BiCMOS technologies. However, these test structures also provide statistical parameters resulting from 'integrated', i.e., multiple processes. This paper examines how in-line testing of presently available WLR test structures can enable the process engineer to uniquely examine various process control parameters. Results in the form of tables and control charts show that implementation of WLR in the manufacturing area provide data useful to process, equipment, and reliability engineers.
Modelling and Simulating Innovation Behaviour within Micro-based Correlated Decision Processes. (2001)

The economic evolution and production has been subject to major shocks and structural changes during the last decades. Beside the standard production factors such as the capital and labour force new production factors for taking into account technological progress and knowledge production and knowledge transfer have to be considered. Modelling of the evolution of technologies as well as structural changes in the management of firms however, require a better understanding of knowledge production and the knowledge transfer process within and between industries, the role and efficiency of transfer institutions such as universities, technology-transfer centres and consulting companies. The paper aims to provide a decision-based framework for this kind of complex interwoven processes.
Performance Evaluation of Sensor Combinations on Mobile Robots for Automated Platoon Control (2004)

interest lies in the area of sensor integration for intelligent transportation systems. Dr. Elizabeth Cannon is Professor and Head of Geomatics
Molecular and immunological characterization of Babesia gibsoni and Babesia microti heat shock protein‐70 (2009)

Serological immunoscreening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to a full-length cDNA containing an open reading frame of 1968 bp predicted to result in a 70-kDa mature protein consisting of 656 amino acids. Analysis of the expression levels of BgHSP-70 indicated elevated transcription from cultured parasites incubated at 40 degrees C for 1 h, but not at 30 degrees C. Interestingly, antiserum raised against recombinant BgHSP-70 protein reacted specifically not only with a 70-kDa protein of B. gibsoni but also with a corresponding native protein of B. microti (BmHSP-70), indicating the high degree of conservation of this protein. The BmHSP-70 gene was then isolated and characterized and the immunoprotective properties of recombinant BgHSP-70 (rBgHSP-70) and rBmHSP-70 were compared in vitro and in vivo. Both proteins had potent mitogenic effects on murine and canine mononuclear cells as evidenced by high proliferative responses and IFN-gamma production after stimulation. Immunization regimes in BALB/c and C57BL/6 mice using rBgHSP-70 and rBmHSP-70 elicited high antibody levels, with concurrent significant reductions in peripheral parasitaemias. Taken together, these results emphasize the potential of HSP-70s as a molecular adjuvant vaccine.
Controlling quality‐of‐service in distributed real‐time and embedded systems via adaptive middleware (2006)

Abstract Computing systems are increasingly distributed, real‐time, and embedded (DRE) and must operate under highly unpredictable and changeable conditions. A challenging problem for DRE systems is adaptation of behavior and reconfiguration of resources to maintain the best application performance in the face of changes in system load and available resources. To provide predictable mission‐critical quality of service (QoS) end‐to‐end, QoS‐enabled middleware services and mechanisms have begun to emerge, but they lack support for applications with stringent QoS requirements in changing, dynamic environments. This paper presents two contributions to research on adaptive and reconfigurable DRE systems. First, we describe the structure and functionality of an advanced middleware platform for developing applications that apply various techniques to adapt themselves to changes in resource availability to meet real‐time QoS requirements. Second, we present results of a case study of a multimedia application for Unmanned Aerial Vehicle (UAV) video distribution we developed using this middleware platform in conjunction with QoS‐enabledoperating systems and networking protocols. We describe the design of the multimedia application using our middleware platform and report empirical results showing how adaptive behavior and end‐to‐end resource management techniques are used to reconfigure the system dynamically to meet timeliness requirements. Copyright © 2006 John Wiley &amp; Sons, Ltd.
Targeting Angiogenesis-Dependent Calcified Neoplasms Using Combined Polymer Therapeutics (2009)

This is the first report to describe a new concept of a narrowly-dispersed combined polymer therapeutic designed to target both tumor and endothelial compartments of bone metastases and calcified neoplasms at a single administration. This new approach of co-delivery of two synergistic drugs may have clinical utility as a potential therapy for angiogenesis-dependent cancers such as osteosarcoma and bone metastases.
Continuous-Flow LVAD Support Causes a Distinct Form of Intestinal Angiodysplasia (2017)

Abnormal intestinal vascular architecture and LVAD-associated vWF degradation were consistent findings in multiple species supported with a continuous-flow LVAD. These are the first direct evidence that LVAD support causes gastrointestinal angiodysplasia. Pathologic vWF metabolism may be a mechanistic link between LVAD support, abnormal angiogenesis, gastrointestinal angiodysplasia, and bleeding.
Quantum Sensing of Free Radicals in Primary Human Dendritic Cells (2021)

Free radicals are crucial indicators for stress and appear in all kinds of pathogenic conditions, including cancer, cardiovascular diseases, and infection. However, they are difficult to detect due to their reactivity and low abundance. We use relaxometry for the detection of radicals with subcellular resolution. This method is based on a fluorescent defect in a diamond, which changes its optical properties on the basis of the magnetic surroundings. This technique allows nanoscale MRI with unprecedented sensitivity and spatial resolution. Recently, this technique was used inside living cells from a cell line. Cell lines differ in terms of endocytic capability and radical production from primary cells derived from patients. Here we provide the first measurements of phagocytic radical production by the NADPH oxidase (NOX2) in primary dendritic cells from healthy donors. The radical production of these cells differs greatly between donors. We investigated the cell response to stimulation or inhibition.
Wearable soft artificial skin for hand motion detection with embedded microfluidic strain sensing (2015)

This paper describes the design and manufacturing of soft artificial skin with an array of embedded soft strain sensors for detecting various hand gestures by measuring joint motions of five fingers. The proposed skin was made of a hyperelastic elastomer material with embedded microchannels filled with two different liquid conductors, an ionic liquid and a liquid metal. The ionic liquid microchannels were used to detect the mechanical strain changes of the sensing material, and the liquid metal microchannels were used as flexible and stretchable electrical wires for connecting the sensors to an external control circuit. The two heterogeneous liquid conductors were electrically interfaced through flexible conductive threads to prevent the two liquid from being intermixed. The skin device was connected to a computer through a microcontroller instrumentation circuit for reconstructing the 3-D hand motions graphically. The paper also presents preliminary calibration and experimental results.
Charge Carrier Molecular Sieve (CCMS) Membranes with Anti-aging Effect for Long-Life Vanadium Redox Flow Batteries (2022)

Vanadium crossover hinders widespread commercial adoption of vanadium redox flow batteries (VRFBs). Superglassy polymers have the potential to offer high selectivity needed to control the crossover but as yet do not possess the requisite proton conductivity and stability. Here, we explore nanocomposite separators that can improve this selectivity. We report a dual-function charge carrier molecular sieve (CCMS) membrane, consisting of a high free volume microporous glassy polymer, poly[1-(trimethylsilyl)-1-propyne] (PTMSP)/sulfonated PAF (PAF-1-SO3H), that effectively hinders the migration of hydrated vanadium ions. Furthermore, ideally placed PAF-1-SO3H pores not only proved excellent for developing proton conductive channels but also suppressed physical aging within the separator. Experiments then linked this to an increased battery cycle life. As a consequence of achieving higher and more stable VRFB performance compared to benchmarked Nafion (Coulombic efficiencies of 97 vs 87% and capacity retention values of 85 vs 58% at a current density of 60 mA cm–2, respectively), our integrated design heralds a class of stable separators for hydrogen-based energy technologies.
p53 is localized to a sub-nucleolar compartment after proteasomal inhibition in an energy-dependent manner (2008)

The tumor suppressor p53 is activated in response to many forms of cellular stress leading to cell cycle arrest, senescence or apoptosis. Appropriate sub-cellular localization is essential for modulating p53 function. We recently showed that p53 localizes to the nucleolus after proteasome inhibition with MG132 and this localization requires sequences within its carboxyl terminus. In the present study, we found that after treatment with MG132, p53 associates with a discrete sub-nucleolar component, the fibrillar center (FC), a region mainly enriched with RNA polymerase I. Moreover, we now demonstrate that this localization is an energy-dependent process as reduction of ATP levels prevents nucleolar localization. In addition, p53 sub-nucleolar accumulation is abolished when cells are subjected to various types of genotoxic stress. Furthermore, we show that monoubiquitination of p53, which causes it to localize to the cytoplasm and nucleoplasm, does not prevent the association of p53 with the nucleolus after MG132 treatment. Importantly, we demonstrate that p53 nucleolar association occurs in lung and bladder carcinomas.