TREATMENT OF HUMAN RENAL CELL CARCINOMA BY A CONDITIONALLY REPLICATING HERPES VECTOR G207 (2001)

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Apr 2001TREATMENT OF HUMAN RENAL CELL CARCINOMA BY A CONDITIONALLY REPLICATING HERPES VECTOR G207 MASAFUMI OYAMA, TAKASHI OHIGASHI, MICHIO HOSHI, MASARU MURAI, KEIICHI UYEMURA, and TAKAHITO YAZAKI MASAFUMI OYAMAMASAFUMI OYAMA More articles by this author , TAKASHI OHIGASHITAKASHI OHIGASHI More articles by this author , MICHIO HOSHIMICHIO HOSHI More articles by this author , MASARU MURAIMASARU MURAI More articles by this author , KEIICHI UYEMURAKEIICHI UYEMURA More articles by this author , and TAKAHITO YAZAKITAKAHITO YAZAKI More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)66512-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Surgical removal remains the only potentially curative therapy for renal cell carcinoma. In this study we evaluated the inhibitory effect of the replication competent engineered herpes simplex virus type 1, G207, for renal cell carcinoma in vitro and in vivo. Materials and Methods: The nature of G207 enables it to replicate within cancer cells, thus, causing cytolysis, but replication is restricted within normal cells. The susceptibility of the human renal cancer cell lines ACHN and A498 to G207 at a multiplicity of infection of 0.1 was examined. In addition, the growth characteristics of G207 was assessed. In vivo athymic mice bearing subcutaneous tumors were inoculated with 1 × 107 plaque forming units of G207 intra-neoplastically. For pathological analysis subcutaneous tumors were stained with X-gal. Results: Two cell lines were efficiently destroyed by G207 within 1 week. The viral yields of G207 increased in a time dependent manner. In vivo the intra-neoplastic inoculation of G207 caused significantly decreased tumor growth in athymic mice harboring subcutaneous human renal cancer cells. On day 14 the mean growth ratio of ACHN and A498 lesions was significantly inhibited in G207 treated compared to control tumors (p <0.005 and <0.0001, respectively). Conclusions: These results suggest that G207 should be considered another potential therapeutic agent for renal cell carcinoma. References 1 : Cancer statistics, 1990. CA Cancer J Clin1990; 40: 9. Google Scholar 2 : Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas. Nat Med1995; 1: 938. Google Scholar 3 : Mapping of herpes simplex virus-1 neurovirulence to gamma 134.5, a gene nonessential for growth in culture. Science1990; 250: 1262. 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J Virol1999; 73: 6319. Google Scholar 21 : Attenuated, replication-competent herpes simplex virus type 1 mutant G207: safety evaluation in mice. J Virol2000; 74: 3832. Google Scholar From the Departments of Physiology, Urology and Neurosurgery, School of Medicine, Keio University, Tokyo, Japan© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 165Issue 4April 2001Page: 1274-1278 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.Keywordsmice, nudeSimplexvirusvirus replicationkidneycarcinoma, renal cellMetricsAuthor Information MASAFUMI OYAMA More articles by this author TAKASHI OHIGASHI More articles by this author MICHIO HOSHI More articles by this author MASARU MURAI More articles by this author KEIICHI UYEMURA More articles by this author TAKAHITO YAZAKI More articles by this author Expand All Advertisement PDF downloadLoading ...