gebhart/patrepeval huggingface dataset:
Chemical Patterning of Ultrathin Polymer Films by Direct-Write Multiphoton Lithography (2011)

We applied 2-photon laser ablation to write subdiffraction nanoscale chemical patterns into ultrathin polymer films under ambient conditions. Poly(ethylene glycol) methacrylate brush layers were prepared on quartz substrates via surface-initiated atom-transfer radical polymerization and ablated to expose the underlying substrate using the nonlinear 2-photon absorbance of a frequency-doubled Ti:sapphire femtosecond laser. Single-shot ablation thresholds of polymer films were ~1.5 times smaller than that of a quartz substrate, which allowed patterning of nanoscale features without damage to the underlying substrate. At a 1/e(2) laser spot diameter of 0.86 μm, the features of exposed substrate approached ~80 nm, well below the diffraction limit for 400 nm light. Ablated features were chemically distinct and amenable to chemical modification.
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites (2017)

Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer metastasis is generally found within the peritoneal cavity rather than other tissues (Lengyel, 2010; Tan <i>et al.</i>, 2006 ). The reason behind this tissue tropism of the peritoneal cavity remains elusive. A prominent feature of this selectivity is ascites, the accumulation of fluid within the peritoneal cavity, containing, amongst others, immune cells, tumor cells and various soluble factors that can be involved in the progression of ovarian cancer ( Kipps <i>et al.</i>, 2013 ). The protocol described here is used to isolate mononuclear cells from ascites to study the functionality of the immune system within the peritoneal cavity.
Immunological responses of mice to lipopolysaccharide: lack of secondary responsiveness by C3H/HeJ mice (1977)

Mice of the C3H/HeJ strain, which were unresponsive to the biological effects of bacterial lipopolysaccharide (LPS), could not be induced to make specific secondary immunological responses to LPS; they responded to two doses of LPS with a primary response. This lack of secondary responsiveness by C3H/HeJ mice was due to a defect in a single, autosomal, dominant gene. Thus, further evidence was provided that an intact second immunological signal and responsiveness thereto were required to trigger secondary antibody responses in primed animals.
Identification and validation of a fractional order dynamic model for a piezoelectric tactile sensor (2010)

This paper presents the identification of a piezoelectric tactile sensor which aims to give the sense of touch to humanoid robots. The sensor has been characterized in frequency domain and the low frequency behavior reveals a non-integer behavior. A simple model based on the a priori knowledge of the sensor and the observation of the frequency response has been hypothesized and a least squares method has been applied to identify the device. The impulse response has been calculated using the Mittag-Leffler function and validation in time domain has been performed. Simulation and experimental results are in good agreement showing that the identified model, despite its simplicity, is able to represent properly the tactile sensor.
Megestrol acetate for estrus postponement in the bitch. (1975)

Megestrol acetate was given orally to 389 bitches in early proestrus, at a dosage of 2.2 mg/kg (1 mg/lb) per day for 8 days. Estrus was suppressed in 357 (92%) of the bitches. Additionally, 119 bitches in anestrus were given the drug at the rate of 0.55 mg/kg (0.25 mg/lb) per day for 32 days. Estrus was suppressed in 115 (98%) of these bitches. Adverse effects were minimal. Pyometra developed in 3 (0.8%) of the 389 bitches treated in early proestrus. The drug also was given to 19 bitches at the rate of 0.55 mg/kg/day for 32 days, regardless of the stage ofting at the 1st posttreatment estrus and 4 after mating at the 2nd posttreatment estrus. Litter size, success in rearing pups, and sex ratios were not significantly different from these factors in 53 litters from untreated bitches.
Growth factor(s) produced during infection with an adenovirus variant stimulates proliferation of nonestablished epithelial cells. (1987)

Infection of primary baby rat kidney cells with an adenovirus variant that encodes only the 12S gene of the E1A region, adenovirus type 5 (Ad5) 12S, results in the production of a growth factor that stimulates primary epithelial cells to proliferate. Increased epithelial cell DNA synthesis and proliferation is detectable between 24 and 36 hr after the addition of conditioned medium from Ad5 12S infected cells and not from cells infected with an E1A deletion mutant virus, Ad5 dl312. This mitogenic factor(s) is effective in the absence of serum and can override the inhibitory effect of serum on primary epithelial cells. Furthermore, there is a requirement for the continued presence of the growth factor(s) in the Ad5 12S conditioned medium to maintain epithelial cell proliferation, and the conditioned medium can maintain these cells in a proliferative state for at least 6 wk. The stimulatory activity in Ad5 12S conditioned medium is associated with large molecular weight complexes, from which it can be released by 4 M NaCl. Several characteristics of the growth factor(s) indicate that it is a unique mitogen for epithelial cells.
Effects on Cytokines and Histology by Treatment with the Ace Inhibitor Captopril and the Antioxidant Retinoic Acid in the Monocrotaline Model of Experimentally Induced Lung Fibrosis (2007)

Monocrotaline (MCT), a pyrrolizidine alkaloid extracted from the shrub Crotalaria spectabilis, induces in the lungs of many mammalian species severe hypertension and fibrosis. Previous work with MCT-induced lung disease in rats has shown that some of the steps to progressive fibrosis can be interrupted or decreased by intervention with retinoic acid (RA) or with the angiotensin converting enzyme inhibitor, captopril. This report emphasizes the pathology and cytokines present in lungs of rats in the MCT model of hypertension and fibrosis in 8 treatment groups, six per group: (1) controls, not treated; (2) captopril; (3) RA; (4) combined captopril and RA. Groups 5-8 replicated groups 1-4 and also received MCT subcutaneously. Tissues were harvested at 28 days for histopathology and measurement of cytokines TGFbeta, TNFalpha, interleukin 6, and IFN_. TGFbeta was depressed at 28 days by MCT, an effect reversed by a combination of captopril and RA. RA influences production of an important Th1 cytokine, IFN_, and demonstrated the greatest limitation of MCT-induced TNFalpha. The MCT-induced lung pathology of vasculitis, interstitial pneumonia and fibrosis was limited by captopril. Smooth muscle actin was overexpressed in MCT treated animals receiving RA, an effect reduced with captopril. Overall, the study confirmed the existence of a protective effect for both captopril and RA from MCT-induced lung damage at 30 days. No synergistic or antagonistic activity was observed when the two drugs were administered together. Each of the drugs exerts different and particular effects on serum and tissue levels of various cytokines, suggesting that each drug is efficient at different points of attack in the control of lung fibrosis.
Is Batch Norm unique? An empirical investigation and prescription to emulate the best properties of common normalizers without batch dependence (2020)

We perform an extensive empirical study of the statistical properties of Batch Norm and other common normalizers. This includes an examination of the correlation between representations of minibatches, gradient norms, and Hessian spectra both at initialization and over the course of training. Through this analysis, we identify several statistical properties which appear linked to Batch Norm's superior performance. We propose two simple normalizers, PreLayerNorm and RegNorm, which better match these desirable properties without involving operations along the batch dimension. We show that PreLayerNorm and RegNorm achieve much of the performance of Batch Norm without requiring batch dependence, that they reliably outperform LayerNorm, and that they can be applied in situations where Batch Norm is ineffective.
Nuclear Instruments and Methods in Physics Research A (2009)

We present recent results on our development of single photon detectors, including: gated and freerunning InGaAs/InP avalanche photodiodes (APDs); hybrid detection systems based on sum-frequency generation (SFG) and Si APDs—SFG‐Si APDs; and SSPDs (superconducting single photon detectors), for telecom wavelengths; as well as SiPM (Silicon photomultiplier) detectors operating in the visible regime.
Adaptive luminance contrast for enhancing reading performance and visual comfort on smartphone displays (2014)

This study developed a model for setting the adaptive luminance contrast between text and background for enhancing reading performance and visual comfort on smartphone displays. The study was carried out in two experiments. In Experiment I, a user test was conducted to identify the optimal luminance contrast with regard to subjects' reading performance, measured by lines of text reading and visual comfort, assessed by selfreport after the reading. Based on the empirical results of the test, an ideal adaptive model which decreases the luminance contrast gradually with passage of time was developed. In Experiment II, a validation test involving reading performance, visual comfort, and physiological stress measured by a brainwave analysis using an electroencephalogram confirmed that the proposed adaptive luminance contrast is adequate for prolonged text reading on smartphone displays. The developed model enhances both reading performance and visual comfort as well as reduces the energy consumption of a smartphone; hence, it is expected that this study will be applied to diverse kinds of visual display terminals.
Best Practices for Meta-Reviews in Physical Activity and Health Research: Insights From the Physical Activity Guidelines for Americans Advisory Committee Scientific Report (2021)

Using examples from the PAGAC Scientific Report, we provide (1) a high-level introduction to MRs; (2) recommended steps in conducting a MR; (3) challenges that can be encountered; and (4) guidance in addressing these challenges.
Artificial Pancreas: Evaluating the ARG Algorithm Without Meal Announcement (2019)

<i>In silico</i> results indicate that it is feasible to perform an in vivo evaluation of the ARG algorithm with unannounced meals.
Graph-based Sybil Detection in Social and Information Systems (2013)

Sybil attacks in social and information systems have serious security implications. Out of many defence schemes, Graph-based Sybil Detection (GSD) had the greatest attention by both academia and industry. Even though many GSD algorithms exist, there is no analytical framework to reason about their design, especially as they make different assumptions about the used adversary and graph models. In this paper, we bridge this knowledge gap and present a unified framework for systematic evaluation of GSD algorithms. We used this framework to show that GSD algorithms should be designed to find local community structures around known non-Sybil identities, while incrementally tracking changes in the graph as it evolves over time.
Laser-induced plasma shock wave excitation technique with acoustic lens (2020)

This paper proposes a methodology to adjust an excitation force amplitude generated by laser-induced plasma shock wave. To realize a remote sensing technique, we investigate a non-contact method to vibrate target mechanical structures or infrastructures to be inspected using laser ablation or laser-induced plasma shock wave. Laser ablation damages an excitation point of the structures because the laser beam is irradiated to the point. Laser-induced plasma shock wave is completely non-destructive method, but the amplitude of the excitation force of laser-induced plasma shock wave is less than that of laser ablation. Furthermore, laser-induced plasma shock wave is spherical, and only a portion of the wave is used to vibrate the target structures. In our experiment, an acoustic lens is used to focus laser-induced plasma shock wave, allowing a control the amplitude of the excitation force of that.
In Vitro Treatment of Melanoma Brain Metastasis by Simultaneously Targeting the MAPK and PI3K Signaling Pathways (2014)

Malignant melanoma is the most lethal form of skin cancer, with a high propensity to metastasize to the brain. More than 60% of melanomas have the BRAFV600E mutation, which activates the mitogen-activated protein kinase (MAPK) pathway [1]. In addition, increased PI3K (phosphoinositide 3-kinase) pathway activity has been demonstrated, through the loss of activity of the tumor suppressor gene, PTEN [2]. Here, we treated two melanoma brain metastasis cell lines, H1_DL2, harboring a BRAFV600E mutation and PTEN loss, and H3, harboring WT (wild-type) BRAF and PTEN loss, with the MAPK (BRAF) inhibitor vemurafenib and the PI3K pathway associated mTOR inhibitor temsirolimus. Combined use of the drugs inhibited tumor cell growth and proliferation in vitro in H1_DL2 cells, compared to single drug treatment. Treatment was less effective in the H3 cells. Furthermore, a strong inhibitory effect on the viability of H1_DL2 cells, when grown as 3D multicellular spheroids, was seen. The treatment inhibited the expression of pERK1/2 and reduced the expression of pAKT and p-mTOR in H1_DL2 cells, confirming that the MAPK and PI3K pathways were inhibited after drug treatment. Microarray experiments followed by principal component analysis (PCA) mapping showed distinct gene clustering after treatment, and cell cycle checkpoint regulators were affected. Global gene analysis indicated that functions related to cell survival and invasion were influenced by combined treatment. In conclusion, we demonstrate for the first time that combined therapy with vemurafenib and temsirolimus is effective on melanoma brain metastasis cells in vitro. The presented results highlight the potential of combined treatment to overcome treatment resistance that may develop after vemurafenib treatment of melanomas.
The Analysis of Stability in a Steam Generator (1985)

The purpose of this paper is to investigate the density-wave oscillation type instability in the recirculating loop of U-tube steam generator (UTSG). The perturbed and nodalized conservations equations based on the drift-flux model have been derived to obtain the single-and two-phase pressure drop perturbations, by taking into account the slip between phases, nonuniform heat flux and heated wall dynamics. To assess the stability, the frequency domain technique with the Nyquist criterion has been used under the constant pressure drop boundary condition through the loop. The computer implementation of this model, SASG, was used for the parametric study of the steam generator in Kori-Unit 1. The results of the parametric study revealed important factors influencing UTSG stability margin.
Real-time wireless decision support alerts on a Palmtop PDA. (1995)

The authors devised a system which continuously analyzes data exported from a Clinical Information System for the occurrence of exceptional or life-threatening clinical events. A configurable rule-based system was created to detect and act on such events. When detected, the system formats an alerting message, dials a modem and transmits the message to a commercial satellite relay system. Ennunciated by an alerting beep and blinking LED on a PCMCIA receiver card, the alert message appears on the screen of a Palmtop Personal Digital Assistants (PDA) carried by designated clinicians.
Objektivierung des Therapieerfolges einer spezifischen Immuntherapie bei Gräserpollenallergie* (1983)

So far it has not been possible to clearly objectify the success of a specific immunotherapy. The aim of the study is to determine which immunological parameters and which clinically biological observations can be used in order to verify a therapeutic success. In four different groups of patients suffering from grass pollen allergy, different specific immunotherapies were carried out pre-seasonally. Continuous observations of patients symptoms together with continuous measurements of the concentration of pollen in the air, of the usual whereabouts of the respective patients, as well as the determination of specific IgE and IgG antibodies before and after the immunotherapy, and the observation of the IgD titre were used as basis of assessment of the efficacy of the specific immunotherapy. Statistical evaluation showed that only the pollen-exposure measurement as compared to the complaints of the individual patients can yield exact information on the therapeutic success. The immunological parameters, in particular the determination of the specific IgG antibodies, are not statistically related to the actual therapeutic efficacy.
The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family (2013)

Trypanosome protozoan parasites are the causative agents of devastating diseases. Trypanosome telomeres grow in an uncontrolled manner and the variant surface glycoprotein (VSG) genes are located in subtelomeric domains. The gene encoding telomerase reverse transcriptase (TERT) was identified and in this study, we describe the Trypanosoma brucei telomerase RNA (TER). TER RNA is bound by the core proteins of the C/D small nucleolar RNA (snoRNA) family and associates with the methyltransferase-associated protein (MTAP), whose homologue binds to mammalian TER. Silencing of TbTER resulted in telomere shortening. This is the first report of a TER that binds the C/D snoRNA core proteins.
High resolution steady-state measurements of thermal contact resistance across thermal interface material junctions (2017)

Thermal interface materials (TIMs) are meant to reduce the interfacial thermal resistance (R<sub>T</sub>) across bare metal contacts in commercial electronics packaging systems. However, there is little scientific consensus governing material design for optimized thermal performance. This is principally due to the inability to separate the effects of the intrinsic material thermal properties from the magnitude of heat flow crossing the TIM-substrate junction (R<sub>C</sub>). To date, efforts to isolate these effects using standard thermal interface material characterization techniques have not been successful. In this work, we develop an infrared thermography-based steady-state heat meter bar apparatus with a novel in situ thickness measurement system having 0.5 nm sensitivity. These in situ thickness measurements allow us to simultaneously determine R<sub>T</sub> and R<sub>C</sub> independently across current state-of-the-art TIMs with ±5% uncertainty. In this work, thermal pastes with bond line thicknesses ranging between 5 and 50 μm are used to illustrate the capability of the apparatus to measure extremely thin materials that are expected to achieve relatively low values of R<sub>T</sub>. Results suggest that the contribution of the thermal contact resistance to the total thermal resistance can range from 5% to 80% for these materials. This finding highlights the need for appropriate metrology and independent measurements of R<sub>C</sub> and R<sub>T</sub> to better optimize thermal interface materials for a number of important electronics applications.
c‐kitligand combined with GM‐CSF and/or IL‐3 can expand CD34+hematopoietic progenitor subsets for several weeks in vitro (1994)

It might be possible to facilitate engraftment after transplantation of purified hematopoietic progenitor cells if the cells are stimulated ex vivo prior to transplantation. The aim of this study was to analyze the potential of c-kit ligand (CKL) combined with granulocyte-macrophage colony-stimulating factor (GM-CSF) and/or interleukin-3 (IL-3) to induce proliferation and differentiation of human bone marrow CD34+ cells in vitro. Particular attention was paid to the ability to expand populations that could maintain progenitor characteristics, i.e. CD34 expression and generation of colony forming cells (CFC), for a considerable period of time. Purified CD34+ cells were cultured in liquid medium for 42 days interrupted by immunophenotyping and CFC assays. In the presence of CKL combined with GM-CSF and/or IL-3, the total number of cells expressing CD34 increased significantly for several weeks after an initial decline. Further, CFC were continually recovered in these cultures. Based on the kinetics and the flow cytometry analysis, the expanding populations that continued to express CD34 probably originated from noncommitted, immature CD34+ cell subsets. CKL combined with GM-CSF and/or IL-3 also induced strong cell proliferation. The majority of the proliferating cells lost CD34 expression and acquired a series of mature myeloid cell surface markers associated with the monocytic, granulocytic and megakaryocytic lineages. These cells probably originated from committed CD34+ cell subsets. We conclude that CKL combined with GM-CSF and/or IL-3 can stimulate noncommitted, immature as well as committed CD34+ cell populations to expand and to differentiate. This property might be useful in a short-term ex vivo pretransplant stimulation of CD34+ cells in an attempt to facilitate rapid and stable engraftment after stem cell transplantation.
Packet Scheduling Algorithm for Wireless Video Streaming (2002)

We propose a class of packet scheduling algorithms for video streaming over wireless channels by applying different deadline thresholds to video packets (VP) with different importance. The importance of a VP is determined by its relative position within its group of pictures (GOP) and motion-texture context. We assume wireless channels with fixed round trip time, and with a feedback channel available from receiver to sender. Based on the deadline threshold, the sender schedules video packets in a different order from the original playback order. In trace-driven simulations, we evaluate the performance in terms of PSNR, and observe improvements over the conventional earliest-deadline-first schemes. 1.
Homeostasis inside Single Activated Phagolysosomes: Quantitative and Selective Measurements of Submillisecond Dynamics of Reactive Oxygen and Nitrogen Species Production with a Nanoelectrochemical Sensor (2022)

Reactive oxygen and nitrogen species (ROS/RNS) are generated by macrophages inside their phagolysosomes. This production is essential for phagocytosis of damaged cells and pathogens, i.e., protecting the organism and maintaining immune homeostasis. The ability to quantitatively and individually monitor the four primary ROS/RNS (ONOO<sup>-</sup>, H<sub>2</sub>O<sub>2</sub>, NO, and NO<sub>2</sub><sup>-</sup>) with submillisecond resolution is clearly warranted to elucidate the still unclear mechanisms of their rapid generation and to track their concentration variations over time inside phagolysosomes, in particular, to document the origin of ROS/RNS homeostasis during phagocytosis. A novel nanowire electrode has been specifically developed for this purpose. It consisted of wrapping a SiC nanowire with a mat of 3 nm platinum nanoparticles whose high electrocatalytic performances allow the characterization and individual measurements of each of the four primary ROS/RNS. This allowed, for the first time, a quantitative, selective, and statistically robust determination of the individual amounts of ROS/RNS present in single dormant phagolysosomes. Additionally, the submillisecond resolution of the nanosensor allowed confirmation and measurement of the rapid ability of phagolysosomes to differentially mobilize their enzyme pools of NADPH oxidases and inducible nitric oxide synthases to finely regulate their homeostasis. This reveals an essential key to immune responses and immunotherapies and rationalizes its biomolecular origin.