gebhart/patrepeval huggingface dataset:
Plasma concentrations of lignocaine and prilocaine after a 24-h application of analgesic cream (EMLAR) to leg ulcers (2001)

The results indicate that a 24-h application of 5-10 g EMLA results in peak plasma concentrations of the two local anaesthetics, which combined are less than one-fifth of those associated with toxic reactions. The analgesic efficacy of EMLA for the relief of chronic ulcer pain deserves further study.
C3- and C3/C5-Epimerases Required for the Biosynthesis of the Capsular Polysaccharides from Campylobacter jejuni (2022)

<i>Campylobacter jejuni</i> is a human pathogen and one of the leading causes of food poisoning in Europe and the United States. The outside of the bacterium is coated with a capsular polysaccharide that assists in the evasion of the host immune system. Many of the serotyped strains of <i>C. jejuni</i> contain a 6-deoxy-heptose moiety that is biosynthesized from GDP-d-<i>glycero</i>-d-<i>manno</i>-heptose by the successive actions of a 4,6-dehydratase, a C3/C5-epimerase, and a C4-reductase. We identified 18 different C3/C5-epimerases that could be clustered together into three groups at a sequence identity of >89%. Four of the enzymes from the largest cluster (from serotypes HS:3, HS:10, HS:23/36, and HS:41) were shown to only catalyze the epimerization at C3. Three enzymes from the second largest cluster (HS:2, HS:15, and HS:42) were shown to catalyze the epimerization at C3 and C5. Enzymes from the third cluster were not characterized. The three-dimensional structures of the epimerases from serotypes HS:3, HS:23/36, HS:15, and HS:41 were determined to resolutions of 1.5-1.9 Å. The overall subunit architecture places these enzymes into the diverse "cupin" superfamily. Within X-ray coordinate error, the immediate regions surrounding the active sites are identical, suggesting that factors extending farther out may influence product outcome. The X-ray crystal structures are consistent with His-67 and Tyr-134 acting as general acid/base catalysts for the epimerization of C3 and/or C5. Two amino acid changes (A76V/C136L) were enough to convert the C3-epimerase from serotype HS:3 to one that could now catalyze the epimerization at both C3 and C5.
The mononucleotide-dependent, nonantisense mechanism of action of phosphodiester and phosphorothioate oligonucleotides depends upon the activity of an ecto-5′-nucleotidase (2001)

Many reports indicate different nonantisense yet sequence-specific effects of antisense phosphorothioate oligonucleotides. Products of enzymatic degradation of the oligonucleotides can also influence cell proliferation. The cytotoxic effects of deoxyribonucleoside-5'-phosphates (dNMPs) and their 5'-phosphorothioate analogs, deoxyribonucleoside-5'-monophosphorothioates (dNMPSs) on 4 human cell types (HeLa, HL-60, K-562, and endothelial cells) were examined, and the effects were correlated with the catabolism of these compounds. The results indicate that differences in cytotoxicity of dNMPs or dNMPSs in these cells depend upon different activity of an ecto-5'-nucleotidase. It has also been found that dNMPSs stimulate proliferation of human umbilical vein endothelial cells and HL-60 cells in a concentration-dependent manner. This stimulation might be caused by the binding of deoxynucleoside-5'-phosphorothioates to as-yet unidentified nucleotide receptor(s) at the cell surface. (Blood. 2001;98:995-1002)
Currents status and strategies for the teaching of selective course of neuroanatomy for medical graduates (2012)

Selective courses are different from compulsory courses in teaching objective,students' study demand,teaching content.Different teaching designs should be used according to different teaching objectives in selective courses for graduates.Targeted scientific thoughts and methodological training should be designed with respect to the fact that students will begin their experimental studies after the selective lessons.We analyzed the current status of selective course of neuroanatomy for graduates and proposed some solutions.

Key words: 
Medical graduate;  Selective course;  Neuroanatomy
A modified state reduction algorithm for computing weight enumerators for convolutional codes (2005)

The weight enumerator of a convolutional code is an important function that characterizes the codeword distance distribution and allows the error probability bounds of the code to be conveniently computed. An efficient state reduction algorithm to compute weight enumerators by iteratively modifying the symbolic adjacency matrix associated with the code was recently introduced. In this paper, we propose a dynamic elimination ordering technique that exploits the state diagram structure of the convolutional encoder for improving the efficiency of the state reduction algorithm.
Dimeticone 4% liquid gel found to kill all lice and eggs with a single 15 minute application (2011)

Current Controlled Trials ISRCTN59227204.
The Interaction of LEDGF/p75 with Integrase Is Lentivirus-specific and Promotes DNA Binding (2005)

We have previously shown that the p75 isoform of the transcriptional co-activator lens epithelium-derived growth factor (LEDGF) interacts tightly with human immunodeficiency virus (HIV)-1 integrase (IN) and is essential for nuclear targeting of this protein in human cells (Cherepanov, P., Maertens, G., Proost, P., Devreese, B., Van Beeumen, J., Engelborghs, Y., De Clercq, E., and Debyser, Z. (2003) J. Biol. Chem. 278, 372-381; Maertens, G., Cherepanov, P., Pluymers, W., Busschots, K., De Clercq, E., Debyser, Z., and Engelborghs, Y. (2003) J. Biol. Chem. 278, 33528-33539). Here the interaction between recombinant LEDGF/p75 and HIV-1 IN was examined in a pull-down binding test. LEDGF/p75 was shown to increase the solubility of HIV-1 IN. Next, fluorescent correlation spectroscopy was used to measure the interaction of LEDGF/p75 or the complex of HIV-1 IN and LEDGF/p75 with a specific double-stranded DNA oligonucleotide. Whereas LEDGF/p75 displayed only a moderate affinity for DNA, it strongly promoted the binding of HIV-1 IN to DNA. This effect was specific for the p75 isoform of LEDGF and was not seen with p52. In the pull-down assay LEDGF/p75 interacted with HIV-1, HIV-2, and feline immunodeficiency virus IN, but not with the IN of human T-cell lymphotropic virus type 2, Moloney murine leukemia virus, or Rous sarcoma virus. These results strongly suggest that the interaction of LEDGF/p75 with IN is specific to lentiviridae. LEDGF/p75 stimulated the binding of HIV-1 and HIV-2 IN, but not Moloney murine leukemia virus or Rous sarcoma virus IN, to an aspecific DNA. These results provide supporting evidence for our hypothesis that LEDGF/p75 plays a role in the tethering of lentiviral IN to the chromosomal DNA.
Nephrogenic Diabetes Insipidus st[Water and Urea Transport (2007)

Nephrogenic diabetes insipidus (NDI) is a rare disorder in which the kidney is unresponsive to the water-retaining action of vasopressin (antidiuretic hormone). Congenital NDI results from a genetic mutation in the V2-receptor, AQP2, or other transport proteins, such as the UT-B urea transporter, involved in generating a hypertonic renal medulla. Acquired NDI most commonly results from lithium therapy, but can also result from prolonged hypercal-cemia, protein malnutrition, hypokalemia, and following the release of unilateral or bilateral ureteral obstruction. The current therapeutic options for congenital NDI are limited and only partially beneficial in reducing the excessive urine output.
Signal integration and information transfer in an allosterically regulated network (2019)

A biological reaction network may serve multiple purposes, processing more than one input and impacting downstream processes via more than one output. These networks operate in a dynamic cellular environment in which the levels of network components may change within cells and across cells. Recent evidence suggests that protein concentration variability could explain cell fate decisions. However, systems with multiple inputs, multiple outputs, and changing input concentrations have not been studied in detail due to their complexity. Here, we take a systems biochemistry approach, combining physiochemical modeling and information theory, to investigate how cyclooxygenase-2 (COX-2) processes simultaneous input signals within a complex interaction network. We find that changes in input levels affect the amount of information transmitted by the network, as does the correlation between those inputs. This, and the allosteric regulation of COX-2 by its substrates, allows it to act as a signal integrator that is most sensitive to changes in relative input levels.
Whole genome resequencing of the human parasite Schistosoma mansoni reveals population history and effects of selection (2016)

Schistosoma mansoni is a parasitic fluke that infects millions of people in the developing world. This study presents the first application of population genomics to S. mansoni based on high-coverage resequencing data from 10 global isolates and an isolate of the closely-related Schistosoma rodhaini, which infects rodents. Using population genetic tests, we document genes under directional and balancing selection in S. mansoni that may facilitate adaptation to the human host. Coalescence modeling reveals the speciation of S. mansoni and S. rodhaini as 107.5-147.6KYA, a period which overlaps with the earliest archaeological evidence for fishing in Africa. Our results indicate that S. mansoni originated in East Africa and experienced a decline in effective population size 20-90KYA, before dispersing across the continent during the Holocene. In addition, we find strong evidence that S. mansoni migrated to the New World with the 16-19th Century Atlantic Slave Trade.
Wall bounded turbulent flows up to high Reynolds numbers: LES resolution assessment (2019)

Paper AIAA 2019-1887
The influence of collector designs on fmax versus ft characteristics for different types of Si-based RF bipolar transistors (2006)

The cut-off frequency ft, the maximum frequency of oscillation fmax and the collector–emitter breakdown voltage BVCEO are computed for various types of Si-based bipolar transistors with different selectively implanted collector (SIC) profiles. In particular, the influence of SIC profiles on ft versus BVCEO and fmax versus BVCEO characteristics is investigated. Subsequently, the fmax versus ft behaviour is discussed. It is shown that for slow transistors (BJTs) there is a trade-off between ft and fmax. However, in the case of faster HBTs this trend can be reversed.
Induction or Augmentation of Labor and Autism (2014)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
Volume Cost Based Mesh Simplification (2009)

We develop a polygonal mesh simplification algorithm using a vertex decimation approach. The novelty in our method lies in (a) a characterization of mesh vertices as hyperbolic or nonhyperbolic based upon their discrete local geometry, (b) the cost function used to select a vertex for decimation, and (c) the heuristics applied to re-triangulate the resulting hole. The algorithm begins by classifying the input mesh vertices as hyperbolic or non-hyperbolic, and then computes a volume cost for each nonhyperbolic vertex, in analogy with spherical volume, to capture the loss of fidelity if that vertex is decimated. Vertices of least volume cost are successively deleted and the resulting hole re-triangulated. Preliminary experiments indicate a performance comparable to that of the best known mesh simplification algorithms.
A Novel Method for Directly Analyzing Asparagine and Glutamine by Means of Gc-Ms. Trimethylsilyl Derivatives of Thiazolinones (1975)

Abstract A novel method to analyze asparagine and glutamine amino acids is presented. TMS-methylthiazolinone derivatives are prepared and hydrolysis of amide side chains is avoided. Products were elucidated by their mass spectra.
Nomegestrol acetate contraceptive implant use by women with sickle cell disease* (1998)

Nomegestrol acetate appears to be a safe and appropriate contraceptive for women with sickle cell disease, showing evidence of being a "stimulant" for F-cell reactivation, independent of F hemoglobin total production increase.
Clinical Applications of Short Non-Coding RNA-Based Therapies in the Era of Precision Medicine (2022)

Traditional targeted therapeutic agents have relied on small synthetic molecules or large proteins, such as monoclonal antibodies. These agents leave a lot of therapeutic targets undruggable because of the lack or inaccessibility of active sites and/or pockets in their three-dimensional structure that can be chemically engaged. RNA presents an attractive, transformative opportunity to reach any genetic target with therapeutic intent. RNA therapeutic design is amenable to modularity and tunability and is based on a computational blueprint presented by the genetic code. Here, we will focus on short non-coding RNAs (sncRNAs) as a promising therapeutic modality because of their potency and versatility. We review recent progress towards clinical application of small interfering RNAs (siRNAs) for single-target therapy and microRNA (miRNA) activity modulators for multi-target therapy. siRNAs derive their potency from the fact that the underlying RNA interference (RNAi) mechanism is catalytic and reliant on post-transcriptional mRNA degradation. Therapeutic siRNAs can be designed against virtually any mRNA sequence in the transcriptome and specifically target a disease-causing mRNA variant. Two main classes of microRNA activity modulators exist to increase (miRNA mimics) or decrease (anti-miRNA inhibitors) the function of a specific microRNA. Since a single microRNA regulates the expression of multiple target genes, a miRNA activity modulator can have a more profound effect on global gene expression and protein output than siRNAs do. Both types of sncRNA-based drugs have been investigated in clinical trials and some siRNAs have already been granted FDA approval for the treatment of genetic, cardiometabolic, and infectious diseases. Here, we detail clinical results using siRNA and miRNA therapeutics and present an outlook for the potential of these sncRNAs in medicine.
Accelerated Surface Measurement Using Wavelength Scanning Interferometer with Compensation of Environmental Noise (2013)

The optical interferometry systems are widely used for surface metrology. However, the environmental noise and the data analysis approach can limit the measurement performance during in- process inspection. This paper introduces a wavelength scanning interferometer (WSI) for micro and nano-scale areal surface measurement. The WSI can measure large discontinuous surface profiles without phase ambiguity problems. The proposed WSI is immune to environmental noise using an active servo control system that serves as a phase compensating mechanism. Furthermore, a parallel CUDA programming model is presented as a solution to accelerate the computing analysis. The presented system can be used for on-line or in-process measurement on the shop floor.
Phosphoinositide 3‐kinase: a key biochemical signal for cell migration in response to chemokines (2000)

Chemokines can couple to distinct signalling pathways that have been demonstrated to mediate not only migration, but also cell growth and transcriptional activation. One particular signalling pathway, namely that controlled by the lipid kinase phosphoinositide 3-kinase (PI3K), has been the focus of much attention with respect to its activation by chemokine receptors and the role it plays in regulating cell migration. Identification of PI3K is arguably one of the most exciting recent developments in biochemical signalling. Pharmacological and genetic studies have now convincingly shown that both CC and CXC chemokines stimulate PI3K-dependent chemotaxis of inflammatory cells such as eosinophils, macrophages, neutrophils and T lymphocytes. This review considers the role of specific sub-classes of PI3Ks (e.g. the p85/p110 heterodimer, PI3Kgamma and PI3K-C2alpha) as well as their downstream effector targets in mediating chemokine-stimulated cell migration.
Chaotic complex spreading sequences for asynchronous DS-CDMA. Part II. Some theoretical performance bounds (1998)

For pt.I see G. Mazzini et al., vol.44, pp.937-47 (Oct. 1997). This paper and its companion (Part I) are devoted to the evaluation of the impact of chaos-based techniques on communications systems with asynchronous Code Division Multiple Access. In Part I, a performance index was introduced and exploited to a priori estimate the performance of DS-CDMA communications systems based on chaotic spreading sequences, and to compare it to that of conventional systems. Here, tools from nonlinear dynamical system theory are employed to give a formal ground for those results. Analytical bounds on the expected partial cross correlation between spreading sequences obtained by quantizing and repeating a chaotic time series are derived, ensuring general applicability of such a technique in a real environment. Further analytical arguments guarantee that, when particular chaotic generators are used, expected performance is not worse than that of a well-behaving communications system. This analysis ensures also that, unlike conventional sequences, chaotic spreading codes can be generated for any number of users and allocated bandwidth.
Determination of Number-Average Molecular Weights by Ebulliometry (1973)

This paper describes an ebulliometric system for routine and special determinations of molecular weights. The system uses a simple ebulliometer, an immersion heater, and a Cottrell-type pump. Temperature sensing is by differential thermopile. Precision varies from about 1 to 6%, and values compare well with those from other laboratories and those from other methods. Values as high as 170,000 have been successfully measured. Some problems encountered in using the ebulliometric method are: selection and effect of reference temperature, limitations of the vapor lift pump and a possible substitute for it, measurement of equilibrium concentrations within the operating ebulliometer, and the experimentally determined ebulliometric constant and some factors which influence its value.
Elimination of Hall Probe Orientation Error in Measured Magnetic Field of the Edge-focusing Wiggler (2010)

The edge-focusing (EF) wiggler has been fabricated to evaluate its performance rigorously with the magnetic field measurement. It is a 5-period planar wiggler with an edge angle of 2° and a period length of 60 mm. The magnetic field is measured using Hall probes at four different wiggler gaps. It is experimentally confirmed that a high field gradient of 1.0 T/m is realized, as designed, along the beam axis. The magnetic field gradient of the EF wiggler is derived as a function of the magnetic gap. The field gradient decreases with increasing magnet gap more slowly than the peak magnetic field does for the present experimental model. An analytic formula for the field gradient of the EF wiggler is derived and it is shown that the slope of the field gradient with the magnet gap can be changed by varying the magnet width of the EF wiggler. We analyzed the relation between the orientation errors of the measurement system and the measured magnetic field or field gradient using a model magnetic field of the EF wiggle. We corrected the measurement magnetic field based on this analysis and evaluated the performance of the EF wiggler.
Limitations of Mass Spectrometry-Based Peptidomic Approaches (2015)

Mass spectrometry-based peptidomic approaches are powerful techniques to detect and identify the peptide content of biological samples. The present study investigated the limitations of peptidomic approaches using trimethylammonium butyrate isotopic tags to quantify relative peptide levels and Mascot searches to identify peptides. Data were combined from previous studies on human cell lines or mouse tissues. The combined databases contain 2155 unique peptides ranging in mass from 444 to 8765 Da, with the vast majority between 1 and 3 kDa. The amino acid composition of the identified peptides generally reflected the frequency in the Eukaryotic proteome with the exception of Cys, which was not present in any of the identified peptides in the free-SH form but was detected at low frequency as a disulfide with Cys residues, a disulfide with glutathione, or as S-cyanocysteine. To test if the low detection rate of peptides smaller than 500 Da, larger than 3 kDa, or containing Cys was a limitation of the peptidomics procedure, tryptic peptides of known proteins were processed for peptidomics using the same approach used for human cell lines and mouse tissues. The identified tryptic peptides ranged from 516 to 2418 Da, whereas the theoretical digest ranged from 217 to 7559 Da. Peptides with Cys were rarely detected and, if present, the Cys was usually modified S-cyanocysteine. Additionally, peptides with mono- and di-iodo Tyr and His were identified. Taken together, there are limitations of peptidomic techniques, and awareness of these limitations is important to properly use and interpret results. Graphical Abstract ᅟ.