gebhart/patrepeval huggingface dataset:
Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1 (2016)

Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (mDA) neurons for cell replacement therapy for Parkinson's disease (PD). However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. To eliminate these unwanted cells, cell sorting using antibodies for specific markers such as CORIN or ALCAM has been developed, but neither marker is specific for ventral midbrain. Here we employ a double selection strategy for cells expressing both CORIN and LMX1A::GFP, and report a cell surface marker to enrich mDA progenitors, LRTM1. When transplanted into 6-OHDA-lesioned rats, human iPSC-derived LRTM1<sup>+</sup> cells survive and differentiate into mDA neurons in vivo, resulting in a significant improvement in motor behaviour without tumour formation. In addition, there was marked survival of mDA neurons following transplantation of LRTM1<sup>+</sup> cells into the brain of an MPTP-treated monkey. Thus, LRTM1 may provide a tool for efficient and safe cell therapy for PD patients.
Treatment of Cryptogenic Stroke Patients with Atrial Fibrillation Detected by Insertable Cardiac Monitors Reduces Recurrent Stroke Risk to Background Levels (2021)

Atrial fibrillation (AF) is a known risk factor of ischemic stroke with a reported fivefold increase in incidence. However, it is not well established whether treatment with oral anticoagulation (OAC) in cryptogenic stroke patients with AF, detected by insertable cardiac monitors (ICMs), reduces the risk of recurrent stroke. We aimed to compare recurrent stroke rates between cryptogenic stroke patients who have AF detected by ICMs and thus started on OAC treatment and those without detected AF. We performed a combined retrospective and prospective analysis of consecutive patients who received an ICM indicated for cryptogenic stroke and were followed up with between July 2015 and November 2019. Patients with a prior documented history of AF were excluded. All patients were required to have a home remote monitoring system. We calculated the rates of AF detection and OAC initiation, then compared recurrent annualized stroke rates (ASRs) between patients with and without AF detected. A total of 298 patients with ICMs were included in the study [mean ± standard deviation age: 77 ± 11.7 years; female/male: 147/151; virtual CHA<sub>2</sub>DS<sub>2</sub>-VASc score: 4.96 ± 1.28 points]. AF was discovered in 91 patients (~30%) over a mean 19.3 months follow-up. Of those, 65 (71.4%) were started on OAC, 12 (13.2%) were already on OAC, and 10 (11%) remained non-anticoagulated. In four (4.4%) patients, OAC was started after recurrent stroke when AF was diagnosed. A total of 24 of 298 patients developed recurrent strokes (ASR: 5.0%). Among the 24 patients with recurrent strokes, four had new AF and were on OAC (ASR: 3.23%), six had new AF and were not anticoagulated (ASR: 26.62%), and 14 had no AF detected and no OAC (ASR: 4.20%). Our study found new AF detected by ICMs in almost one-third (30%) of cryptogenic stroke patients (consistent with previous studies), and the majority of them (89%) received OACs. There was no significant difference in the recurrent stroke rate among patients without AF detected and those with AF detected and on OAC. Rigorous arrhythmia monitoring using ICMs can increase new AF detection rates in cryptogenic stroke patients, thereby allowing early initiation of OACs, ultimately reducing the risk of recurrent stroke to background levels.
Preparation and derivatives of cis-M(CO)4(SiCl3)2 (M = iron, ruthenium, osmium) (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and derivatives of cis-M(CO)4(SiCl3)2 (M = iron, ruthenium, osmium)R. K. Pomeroy and K. S. WijesekeraCite this: Inorg. Chem. 1980, 19, 12, 3729–3735Publication Date (Print):December 1, 1980Publication History Published online1 May 2002Published inissue 1 December 1980https://pubs.acs.org/doi/10.1021/ic50214a031https://doi.org/10.1021/ic50214a031research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The accuracy of several multiple sequence alignment programs for proteins (2006)

Our results indicate that employing Simprot's simulated sequences allows the creation of a more flexible and broader range of alignment classes than the usual methods for alignment accuracy assessment. Simprot also allows for a quick and efficient analysis of a wider range of possible evolutionary histories that might not be present in currently available alignment sets. Among the nine programs tested, the iterative approach available in Mafft (L-INS-i) and ProbCons were consistently the most accurate, with Mafft being the faster of the two.
Utility of cardiac computed tomography to identify arrhythmia substrates for ventricular tachycardia and sudden cardiac death (2016)

Sudden cardiac death (SCD) is the leading cause of death in the U.S., and many of these events are attributable to malignant ventricular arrhythmias such as sustained ventricular tachycardia (VT). Most of the efforts to identify arrhythmia precipitants in these patients are based on imaging to look for myocardial or coronary artery disease. As advances in cardiac computed tomography (CCT) are made, it has demonstrated its usefulness in identifying structural intracardiac pathology and measuring parameters of cardiac anatomy and function. In this article we review the different etiologies of VT/SCD that are identifiable by CCT, and its potential usefulness in the workup for VT/SCD.
Opportunities for Wideband Wavelength Conversion in Foundry-Compatible Silicon Waveguides Covered With Graphene (2015)

We numerically examine the opportunities for wideband wavelength conversion through four-wave mixing (FWM) in a foundry-compatible 220-nm-thick silicon-on-insulator (SOI) waveguide covered with the highly nonlinear two-dimensional material of graphene. As a case study, we consider a foundry-compatible SOI waveguide shaped as a double spiral and covered with two separate graphene sheets, which are covered in turn by two solid polymer electrolyte gates. When combining subwatt-level pump powers with a short waveguide length of only a few hundreds of micrometers, “perfectly phase-matched” conversion with significant efficiencies close to -20 dB can be obtained over a more than 40-THz-wide signal band adjacent to the pump frequency. Because of the tunability of the graphene properties using the electrolyte top gates, it is also possible to obtain “quasi-phase matched” FWM conversion through a periodic sign reversal of the graphene third-order nonlinearity along the waveguide. Conversion efficiencies exceeding -30 dB can be achieved over a 3.4-THz-wide signal band that is situated as much as 58 THz away from the pump frequency. Finally, the tunability of the graphene also allows for switching the converter from the perfectly phase-matched to the quasi-phase-matched operation mode.
VENTRAL HERNIA: A DEVICE TO STRENGTHEN THE ABDOMINAL WALL (1918)

VENTRAL HERNIA.[SPT.2I, Igr8 H.'s culture from faeces was then employed and a fairly thick enmulsion from an agar slope yielded with-the serums the following results: H.'s Bacilluts wlith1 Serums. 1 in 25. 1 in 50.l in 125.D. H.'s serum ... .
Observations on the Economics of Dyeing with Reactive Dyes (1962)

The introduction of reactive dyes has significantly affected the established economic relationship between batch and continuous processing. Reactive dyes have properties which are suited to high‐speed continuous dyeing without the need for specialised equipment. On the other hand, conventional dyes, e.g. direct and vat dyes, are designed primarily for batch‐dyeing methods. It is found that the direct production costs for the continuous dyeing of reactive dyes are comparable with those for dyeing direct dyes on the jig or winch. This fact, coupled with the high wet‐fastness properties of reactive dyes, is likely to accelerate the growth of more productive dyeing processes throughout the world.
Parameter Sharing is Surprisingly Useful for Multi-Agent Deep Reinforcement Learning. (2020)

Nonstationarity is a fundamental problem in cooperative multi-agent reinforcement learning (MARL)--each agent must relearn information about the other agent's policies due to the other agents learning, causing information to ring between agents and convergence to be slow. The MAILP model, introduced by Terry and Grammel (2020), is a novel model of information transfer during multi-agent learning. We use the MAILP model to show that increasing training centralization arbitrarily mitigates the slowing of convergence due to nonstationarity. The most centralized case of learning is parameter sharing, an uncommonly used MARL method, specific to environments with homogeneous agents, that bootstraps a single-agent reinforcement learning (RL) methods and learns an identical policy for each agent. We experimentally replicate the result of increased learning centralization leading to better performance on the MARL benchmark set from Gupta et al. (2017). We further apply parameter sharing to 8 modern single-agent deep RL (DRL) methods for the first time in the literature. With this, we achieved the best documented performance on a set of MARL benchmarks and achieved upto 44 times more average reward in as little as 16% as many episodes compared to documented parameter sharing arrangement. We finally offer a formal proof of a set of methods that allow parameter sharing to serve in environments with heterogeneous agents.
Topological phenomena in classical optical networks (2017)

We propose a scheme to realize a topological insulator with optical-passive elements and analyze the effects of Kerr nonlinearities in its topological behavior. In the linear regime, our design gives rise to an optical spectrum with topological features and where the bandwidths and bandgaps are dramatically broadened. The resulting edge modes cover a very wide frequency range. We relate this behavior to the fact that the effective Hamiltonian describing the system's amplitudes is long range. We also develop a method to analyze the scheme in the presence of a Kerr medium. We assess robustness and stability of the topological features and predict the presence of chiral squeezed fluctuations at the edges in some parameter regimes.
Seropersistence of tick-borne encephalitis antibodies, safety and booster response to FSME-IMMUN®0.5 ml in adults aged 18-67 years (2009)

A clinical study was carried out to evaluate the persistence of tick-borne encephalitis (TBE) antibodies 2 and 3 years after a primary vaccination series (three-dose regimen), and to assess the antibody response to a booster vaccination with FSME-IMMUN. Volunteers (N = 347, 18-67 years) who had received two doses of either FSME-IMMUN or Encepur adults and a third vaccination with FSME-IMMUN were enrolled. Seropositivity rates were assessed by ELISA and neutralization test (NT). After the primary series, seropositivity rates were 99.1% as determined by ELISA and 100% by NT, decreasing to 85% and 96.8% in the first two years and to 88.7% and 95.4% after 3 years. Following booster vaccination, 100% of subjects were seropositive. Age was the only variable with a significant influence on the probability of remaining TBE seropositive 2 or 3 years after the third vaccination. In subjects aged 18-50 years, the pre-booster seropositivity rate was higher (88.7% and 92.3% after 2 and 3 years, respectively) than in those aged 51-67 years (65.5% and 70.9% after 2 and 3 years, respectively). Adverse events after booster vaccination occurred with a low frequency and were predominantly mild. An annual TBE antibody decline rate of 0.58 (based on NT) was estimated to lead to antibody titer decrease from e.g., 260 to 45.6 after 3 years. To conclude, a booster vaccination with FSME-IMMUN, administered 3 years after primary vaccination, is well tolerated and induces a
In-situ, non-destructive identification of chemical elements by means of portable EDXRF spectrometer (1999)

The performances of a new portable EDXRF (Energy Dispersive X-ray Fluorescence) Spectrometer designed for in-situ, non-destructive identification of chemical elements in materials are here described. The instrument, based on a Silicon Drift Detector cooled by a Peltier element, does not require a liquid nitrogen cooling system. The energy resolution of the spectrometer is typically 155 eV FWHM at 6 keV at a temperature of about -8/spl deg/C and the peak to valley ratio is better than 10000. The paper reports on the most significant results recently obtained, by using a new version of the Silicon Drift Detector, in measurements carried out "on-the-field" on samples of different materials. The results of the first quantitative analyses of metal alloys carried out with this instrument are also presented.
DrosophilaWntD is a target and an inhibitor of the Dorsal/Twist/Snail network in the gastrulating embryo (2005)

The maternal Toll signaling pathway sets up a nuclear gradient of the transcription factor Dorsal in the early Drosophila embryo. Dorsal activates twist and snail, and the Dorsal/Twist/Snail network activates and represses other zygotic genes to form the correct expression patterns along the dorsoventral axis. An essential function of this patterning is to promote ventral cell invagination during mesoderm formation,but how the downstream genes regulate ventral invagination is not known. We show here that wntD is a novel member of the Wnt family. The expression of wntD is activated by Dorsal and Twist, but the expression is much reduced in the ventral cells through repression by Snail. Overexpression of WntD in the early embryo inhibits ventral invagination,suggesting that the de-repressed WntD in snail mutant embryos may contribute to inhibiting ventral invagination. The overexpressed WntD inhibits invagination by antagonizing Dorsal nuclear localization, as well as twist and snail expression. Consistent with the early expression of WntD at the poles in wild-type embryos, loss of WntD leads to posterior expansion of nuclear Dorsal and snail expression,demonstrating that physiological levels of WntD can also attenuate Dorsal nuclear localization. We also show that the de-repressed WntD in snail mutant embryos contributes to the premature loss of snail expression, probably by inhibiting Dorsal. Thus, these results together demonstrate that WntD is regulated by the Dorsal/Twist/Snail network,and is an inhibitor of Dorsal nuclear localization and function.
Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli (2019)

Polyketides are a class of specialised metabolites synthesised by both eukaryotes and prokaryotes. These chemically and structurally diverse molecules are heavily used in the clinic and include frontline antimicrobial and anticancer drugs such as erythromycin and doxorubicin. To replenish the clinicians' diminishing arsenal of bioactive molecules, a promising strategy aims at transferring polyketide biosynthetic pathways from their native producers into the biotechnologically desirable host Escherichia coli. This approach has been successful for type I modular polyketide synthases (PKSs); however, despite more than 3 decades of research, the large and important group of type II PKSs has until now been elusive in E. coli. Here, we report on a versatile polyketide biosynthesis pipeline, based on identification of E. coli-compatible type II PKSs. We successfully express 5 ketosynthase (KS) and chain length factor (CLF) pairs-e.g., from Photorhabdus luminescens TT01, Streptomyces resistomycificus, Streptoccocus sp. GMD2S, Pseudoalteromonas luteoviolacea, and Ktedonobacter racemifer-as soluble heterodimeric recombinant proteins in E. coli for the first time. We define the anthraquinone minimal PKS components and utilise this biosynthetic system to synthesise anthraquinones, dianthrones, and benzoisochromanequinones (BIQs). Furthermore, we demonstrate the tolerance and promiscuity of the anthraquinone heterologous biosynthetic pathway in E. coli to act as genetically applicable plug-and-play scaffold, showing it to function successfully when combined with enzymes from phylogenetically distant species, endophytic fungi and plants, which resulted in 2 new-to-nature compounds, neomedicamycin and neochaetomycin. This work enables plug-and-play combinatorial biosynthesis of aromatic polyketides using bacterial type II PKSs in E. coli, providing full access to its many advantages in terms of easy and fast genetic manipulation, accessibility for high-throughput robotics, and convenient biotechnological scale-up. Using the synthetic and systems biology toolbox, this plug-and-play biosynthetic platform can serve as an engine for the production of new and diversified bioactive polyketides in an automated, rapid, and versatile fashion.
The study of family organization of formal organization (2002)

Informal organization appears with formal organization operation. Family organization is a special informal organization, it is different with informal organization. They interfere with activity and efficiency of formal organization. It is the significance of studying family organization that take advantage of informal organization, family organization and eliminate their unfavorable influence to achieving the objective of formal organization.
Use of speckle-tracking echocardiography–derived strain and systolic strain rate measurements to predict rejection in transplant hearts with preserved ejection fraction (2018)

2D-STE may be useful in detecting severe transplant rejection in heart transplant patients with normal LVEF.
Fashionpedia-Ads: Do Your Favorite Advertisements Reveal Your Fashion Taste? (2023)

Consumers are exposed to advertisements across many different domains on the internet, such as fashion, beauty, car, food, and others. On the other hand, fashion represents second highest e-commerce shopping category. Does consumer digital record behavior on various fashion ad images reveal their fashion taste? Does ads from other domains infer their fashion taste as well? In this paper, we study the correlation between advertisements and fashion taste. Towards this goal, we introduce a new dataset, Fashionpedia-Ads, which asks subjects to provide their preferences on both ad (fashion, beauty, car, and dessert) and fashion product (social network and e-commerce style) images. Furthermore, we exhaustively collect and annotate the emotional, visual and textual information on the ad images from multi-perspectives (abstractive level, physical level, captions, and brands). We open-source Fashionpedia-Ads to enable future studies and encourage more approaches to interpretability research between advertisements and fashion taste.
Use of Conducting Electroactive Polymers for Drug Delivery and Sensing of Bioactive Molecules. A Redox Chemistry Approach (2000)

We have examined the properties of polypyrrole (PPy) as a model electroactive membrane which can simultaneously serve as a medium sensing, and bioactive molecule releasing, material using optical spectroscopic, potentiometric, and conductometric methods. In particular, PPy membranes can sense hydrazine in aqueous solution with linear logarithmic potentiometric and conductometric responses between 10-4 and 10-1 M. The sensing properties of the PPy membranes are discussed in terms of both its redox properties and specific acid−base behavior. Adenosine triphosphate (ATP) has been used as a model drug which is easily loaded into PPy during electrochemical synthesis. ATP release processes from PPy/ATP membranes have been studied spectroscopically using electrochemical and chemical triggering. Electrochemical triggering allowed ATP to be delivered with a variety of release profiles and adjustable rates (up to 20 μg cm-2 min-1 for a 10 μm thick membrane). The mass transfer through the membranes has been successfully treated using a simple diffusion model (D ∼ 5 × 10-9 cm2 s-1) and discussed with regards to the polymer's structure and potential drug delivery device applications. Hydrazine (0.1 M) and alkaline medium (pH 12) have been used as chemical triggers for ATP release from PPy/ATP membranes. The amount of ATP released was reduced relative to the electrochemically released amount due to inhibited diffusion of reagents into the membranes. The release profiles have been established and demonstrate the viability of a controlled-delivery device using conducting polymers.
Nanocelluloses: Sustainable Design for the Direct Fabrication and Highly Versatile Functionalization of Nanocelluloses (Global Challenges 7/2017) (2017)

A sustainable concept for the scalable direct fabrication and functionalization of nanocellulose with reduced energy consumption is disclosed. A central part is the use of small organic molecules as catalysts and mediators for the production and subsequent versatile surface modification of the cellulosic nanomaterials with catalysts, biologically active, fluorescent and hydrophobic molecules. More information can be found in in article number 1700045 by Samson Afewerki, Rana Alimohammadzadeh, Sinke H. Osong, Cheuk-Wai Tai, Per Engstrand, and Armando Córdova*.
Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements (2021)

Nitrate ions (NO3<sup>-</sup>) were once thought to be inert end products of nitric oxide (NO) metabolism. However, previous studies demonstrated that nitrate ions can be converted back to NO in mammals through a two-step reduction mechanism: nitrate being reduced to nitrite (NO2<sup>-</sup>) mostly by oral commensal bacteria, then nitrite being reduced to NO by several mechanisms including via heme- or molybdenum-containing proteins. This reductive nitrate pathway contributes to enhancing NO-mediated signaling pathways, particularly in the cardiovascular system and during muscular exercise. The levels of nitrate in the body before such utilization are determined by two different sources: endogenous NO oxidation and dietary nitrate intake, principally from plants. To elucidate the complex NO cycle in physiological circumstances, we have examined further the dynamics of its metabolites, nitrate and nitrite ions, which are relatively stable compared to NO. In previous studies skeletal muscle was identified as a major storage organ for nitrate ions in mammals, as well as a direct source of NO during exercise. Therefore, establishing a reliable methodology to measure nitrate and nitrite levels in skeletal muscle is important and should be helpful in extending its application to other tissue samples. This paper explains in detail the preparation of skeletal muscle samples, using three different homogenization methods, for nitrate and nitrite measurements and discusses important issues related to homogenization processes, including the size of the samples. Nitrate and nitrite concentrations have also been compared across four different muscle groups.