gebhart/patrepeval huggingface dataset:
Green MIP-202(Zr) Catalyst: Degradation and Thermally Robust Biomimetic Sensing of Nerve Agents (2021)

Rapid and robust sensing of nerve agent (NA) threats is necessary for real-time field detection to facilitate timely countermeasures. Unlike conventional phosphotriesterases employed for biocatalytic NA detection, this work describes the use of a new, green, thermally stable, and biocompatible zirconium metal-organic framework (Zr-MOF) catalyst, MIP-202(Zr). The biomimetic Zr-MOF-based catalytic NA recognition layer was coupled with a solid-contact fluoride ion-selective electrode (F-ISE) transducer, for potentiometric detection of diisopropylfluorophosphate (DFP), a F-containing G-type NA simulant. Catalytic DFP degradation by MIP-202(Zr) was evaluated and compared to the established UiO-66-NH<sub>2</sub> catalyst. The efficient catalytic DFP degradation with MIP-202(Zr) at near-neutral pH was validated by <sup>31</sup>P NMR and FT-IR spectroscopy and potentiometric F-ISE and pH-ISE measurements. Activation of MIP-202(Zr) using Soxhlet extraction improved the DFP conversion rate and afforded a 2.64-fold improvement in total percent conversion over UiO-66-NH<sub>2</sub>. The exceptional thermal and storage stability of the MIP-202/F-ISE sensor paves the way toward remote/wearable field detection of G-type NAs in real-world environments. Overall, the green, sustainable, highly scalable, and biocompatible nature of MIP-202(Zr) suggests the unexploited scope of such MOF catalysts for on-body sensing applications toward rapid on-site detection and detoxification of NA threats.
Ethylene Trimerization Catalysts Based on Chromium Complexes with a Nitrogen-Bridged Diphosphine Ligand Having ortho-Methoxyaryl or ortho-Thiomethoxy Substituents:  Well-Defined Catalyst Precursors and Investigations of the Mechanism (2006)

Chromium-based ethylene trimerization catalyst precursors ((PNPOMe-d12)CrPh3 (4) and (PNPOMe-d12)CrPh2Cl (7)) having a bis(diphenylphosphino)amine ligand (o-CD3OC6H4)2PN(CH3)P(o-CD3OC6H4)2 ((PNPOMe-d12) = 1) have been prepared and characterized. A thioether analogue (o-CD3SC6H4)2PN(CH3)P(o-CD3SC6H4)2 ((PNPSMe-d12) = 2) and its triphenylchromium complex (PNPSMe)CrPh3 (5) have also been synthesized. The solid-state structures of 4 and 7 display octahedral geometries with a κ3-(P,P,O) coordination of PNPOMe ligands having chromium−oxygen bond lengths of 2.29−2.44 Å. Compound 5 differs, exhibiting (S,P,S)-κ3 coordination of the PNPSMe ligand. The deuteromethyl groups allow for 2H NMR characterization of these paramagnetic complexes in solution. Dynamic exchange processes occur in solution at room temperature to render all four of the methoxy or thioether groups equivalent on the 2H NMR time scale; two distinct coalescence processes are observed by variable-temperature 2H NMR spectroscopy for all compounds. The neutral species 4 and 7 react with ethylene (1 atm) by insertion into chromium−phenyl bonds with the release of styrene and ethylbenzene, but 1-hexene is not observed under these conditions. Activation of 4 by protonation and activation of 7 by halide abstraction in the presence of ethylene provide active trimerization catalysts that give turnover numbers for 1-hexene as high as 3000 mol 1-hexene·mol-1 Cr. These catalysts display comparable activity and selectivity for 1-hexene compared to the original BP system, where the catalyst is generated in situ from CrCl3(THF)3, 1, and MAO. Both the well-defined systems and the CrCl3(THF)3/PNPOMe/MAO system provide catalysts that undergo an initiation period followed by an apparent first-order decomposition process. Activated complexes 4 and 7 initiate trimerization primarily through ethylene insertion into the chromium−phenyl bond, followed by β-hydrogen elimination and reductive elimination to give the active species, rather than via reductive elimination of biphenyl.
Tonsillectomy versus tonsillotomy for recurrent acute tonsillitis in children and adults (TOTO): study protocol for a randomized non-inferiority trial (2021)

German Clinical Trials Register DRKS00020823 . Registered on 04 September 2020.
Microturbine based distributed generator in smart grid application (2009)

This paper presents modeling and simulation of microturbine (MT) models to analyze its load following performance as distributed energy resource (DER) with general as well as critical priority loads. Two different models MT were considered for performance analysis. The first model consists of speed governor, acceleration control, and temperature control blocks while the other is GAST model of MT. The system comprises of a synchronous generator and a MT coupled to it. Simulations are carried out in islanded and grid-connected mode of the system to observe its behavior when supplying customer&apos;s variable loads. The load following characteristics is observed and validated for this MT-synchronous generator model in Matlab-Simulink environment with power system block sets. This is applicable with combined heat power (CHP) generators both with general fuel as well as bio-fuels. The use of bio-fuels is very much promising for generating green power preventing green house gas emissions for fighting against global warming. But it may take some time to be in the market place for its commercial use. (6 pages)
Establishment and characterization of a human non‐secretory plasmacytoid cell line and its hybridization with human B cells (1983)

A human non-secretory plasmacytoid cell line has been established for 6 years in more than 170 passages. Over 300 passages have been made from several early and late passages. The cell line is karyotypically normal, easily grown and has the characteristic features of a non-secretory plasmablast. Its characteristics suggest its use for hybridization by new methods as well as a study of its secretory defect. HPRT-negative phenotypic mutants can be derived from this line and a single polyploid clone has also been isolated. Hybridization with the HPRT+ and HPRT- lines X human B cells is described.
CO2/CH4 and He/N2 Separation Properties and Water Permeability Valuation of Mixed Matrix MWCNTs-Based Cellulose Acetate Flat Sheet Membranes: A Study of the Optimization of the Filler Material Dispersion Method (2021)

The main scope of this work is to develop nano-carbon-based mixed matrix cellulose acetate membranes (MMMs) for the potential use in both gas and liquid separation processes. For this purpose, a variety of mixed matrix membranes, consisting of cellulose acetate (CA) polymer and carbon nanotubes as additive material were prepared, characterized, and tested. Multi-walled carbon nanotubes (MWCNTs) were used as filler material and diacetone alcohol (DAA) as solvent. The first main objective towards highly efficient composite membranes was the proper preparation of agglomerate-free MWCNTs dispersions. Rotor-stator system (RS) and ultrasonic sonotrode (USS) were used to achieve the nanofillers' dispersion. In addition, the first results of the application of the three-roll mill (TRM) technology in the filler dispersion achieved were promising. The filler material, MWCNTs, was characterized by scanning electron microscopy (SEM) and liquid nitrogen (LN<sub>2</sub>) adsorption-desorption isotherms at 77 K. The derivatives CA-based mixed matrix membranes were characterized by tensile strength and water contact angle measurements, impedance spectroscopy, gas permeability/selectivity measurements, and water permeability tests. The studied membranes provide remarkable water permeation properties, 12-109 L/m<sup>2</sup>/h/bar, and also good separation factors of carbon dioxide and helium separations. Specifically, a separation factor of 87 for 10% He/N<sub>2</sub> feed concentration and a selectivity value of 55.4 for 10% CO<sub>2</sub>/CH<sub>4</sub> feed concentration were achieved.
RT/RSX MULTI: Packages for Data Acquisition and Analysis in High-Energy Physics (1979)

The MULTI system is a software package for data acquisition, data analysis, and experiment control. PDP-11 implementations are in widespread use under the RT-11 and RSX-1lM operating systems at Fermilab and elsewhere. The system philosophy, architecture, and significant features are discussed, and the measured performance is reported.
Molecular tools for the mapping of the human genome. (1985)

The rapid progress in molecular cloning and DNA analysis techniques, together with the use of cloned DNA probes from specific chromosomes of the human genome might allow localisation and ultimately identification of genes defined by single mutations. The discrepancy between genetic dimensions expressed in centimorgans, each corresponding to millions of base pairs and distances easily accessible by molecular techniques amounting to at the most hundreds of kilobase pairs may be bridged with some new cloning techniques partially developed at the European Molecular Biology Laboratory. These techniques were designed to allow rapid cloning and analysis of large regions of mammalian genomes.
Wnt receptor Frizzled 8 is a target of ERG in prostate cancer (2018)

Prostate cancer (PCa) is one of the most frequently diagnosed cancers among men. Many molecular changes have been detailed during PCa progression. The gene encoding the transcription factor ERG shows recurrent rearrangement, resulting in the overexpression of ERG in the majority of prostate cancers. Overexpression of ERG plays a critical role in prostate oncogenesis and development of metastatic disease. Among the downstream effectors of ERG, Frizzled family member FZD4 has been shown to be a target of ERG. Frizzled-8 (FZD8) has been shown to be involved in PCa bone metastasis. In the present study, we show that the expression of FZD8 is directly correlated with ERG expression in PCa. Furthermore, we show that ERG directly targets and activates FZD8 by binding to its promoter. This activation is specific to ETS transcription factor ERG and not ETV1. We propose that ERG overexpression in PCa leads to induction of Frizzled family member FZD8, which is known to activate the Wnt pathway. Taken together, these findings uncover a novel mechanism for PCa metastasis, and indicate that FZD8 may represent a potential therapeutic target for PCa.
Abstract 54: Characterization of patient-derived organoids cultured on a gas-rich, liquid-liquid interface (2019)

Abstract Modeling tissues in vitro has long consisted of two-dimensional (2D) monolayer cultures grown in a dish, flask, or multi-well plate. While these methods remain adequate for some applications, the advent of personalized cancer therapies has necessitated the development of more complex models that more accurately represent the in vivo environment. Attempting to characterize tumor cell biology and response to candidate pharmaceuticals in 2D cultures has led to results that fail to translate to clinical therapies. A key source of this failure is the loss of cell diversity due to selective pressure resulting in cell populations that favor adhering to a rigid substrate. Three-dimensional (3D) cell culture methods have produced models that capture many of the dynamics of the in vivo tissue environment. Patient-derived organoids (PDOs) are quickly becoming the most attractive solution for creating predictive, patient-specific tumor models in vitro. Typically grown as spheroids, these models still present limitations in terms of cell survival and recapitulation of tumor development. Lena Biosciences has previously developed complex, vascularized tissue models using patented 3D scaffolds and a perfusion system. Hepatocytes grown in this system demonstrated increased cell viability and recovery of cytochrome P450 enzyme activity compared to 2D controls. Integral to the perfusion system is a pure, synthetic liquid that is twice as dense as and immiscible with cell culture medium and reagents. It is permissive to atmospheric gases, providing a gas-rich, liquid-liquid interface below 3D cultures maintained in the system. We hypothesized that PDOs could be cultured without a 3D scaffold, directly at the interface between the cell culture medium and this synthetic liquid. A specific preparation of this interface resulted in lung-derived PDOs that spontaneously formed dense, 3D, tissue-like structures that showed increased viability (as measured by a 50% reduction in LDH release) and cellular respiration (three-fold increase as measured by an alamarBlue assay). Ongoing studies are focused on characterizing PDOs from other patients and other tissues as they develop in long-term in vitro studies. Should this PDO model prove to be a faithful representation of in vivo tumor development, this system would be ideal for applications such as testing drug efficacy and studying mechanisms of tumor metastasis. Citation Format: James T. Shoemaker, Katherine R. Richardson, Jamie Arnst, Adam Marcus, Jelena Vukasinovic. Characterization of patient-derived organoids cultured on a gas-rich, liquid-liquid interface [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 54.
Cannabis XIX. Oxygenated 1,2‐Diphenylethanes from Marihuana (1978)

Abstract The presence of several hydroxy‐ and methoxy‐substituted 1,2‐diphenylethanes in Mexican marihuana has been established. The structure of three of these compounds has been elucidated. The products may have physiological activity. Their role as precursors for cannabispiro compounds is discussed.
Establishment and characterization of six human lung cancer cell lines: EGFR, p53 gene mutations and expressions of drug sensitivity genes (2011)

These well-characterized lung cancer cell lines may be useful tools for investigations of the biological characteristics of lung cancers, particularly for investigations related to mutations of EGFR.
Self-Organized Monolayer of Nanosized Ceria Colloids Stabilized by Poly(vinylpyrrolidone) (2006)

Four nanometer colloidal ceria nanocrystals in a fluorite cubic structure have been synthesized via an alcohothermal treatment at 180 degrees C for 24 h from Ce(NO(3))(3)*6H(2)O in ethanol, using various alkylamines including triethylamine, butylamine, and hexadecylamine as the bases and poly(vinylpyrrolidone) (PVP) as the stabilizer. They were characterized by multiple measurements of X-ray diffraction (XRD), transmission electron microscopy (TEM), high-resolution TEM (HRTEM), ultraviolet visible (UV-vis) spectroscopy, dynamic light scattering (DLS), and infrared spectroscopy (IR). The introduction of PVP could effectively stabilize the cerium nuclei against self-aggregation and finally lead to the formation of the CeO(2) colloids. As compared with that of their precipitated counterparts, the UV-vis spectra showed a blue-shifted absorption edge for the as-obtained colloidal nanocrystals, revealing that their surfaces were well-passivated by PVP. Four types of self-organized monolayer patterns (i.e., isolated particles, short chainlike (pseudo-1-D aggregated), pearl necklace-like (1-D aggregated), and dendritic (pseudo-2-D aggregated) alignments) appeared for the as-obtained colloidal particles on the copper TEM grids, due to the delicate balance of the attractive and repulsive forces between the PVP-passivated CeO(2) nanocrystals during the irreversible evaporation of the solvent from various colloidal solutions under ambient conditions. The type of alkylamine and the concentration of PVP were confirmed to be the crucial factors determining the oriented-aggregation dimensionality of the CeO(2) colloids. Possible interparticle interaction modes have been suggested to explain such complex self-organization patterns exhibited by the as-obtained CeO(2) nanocrystals.
A common mitochondrial DNA mutation in the t-RNA(Lys) of patients with myoclonus epilepsy associated with ragged-red fibers. (1990)

Nucleotide sequence analyses of muscle mitochondrial DNA (mtDNA) from a patient with myoclonus epilepsy associated with ragged-red fibers (MERRF) revealed 33 single base substitutions, including 23 in coding regions for mitochondrial polypeptides and 10 in non-coding regions, as compared with the normal human mtDNA sequence. Three substitutions, in COI, ND4, and Cytb, would result in amino acid substitutions, which are conserved among species. Of three patients with MERRF, all had an identical A to G base substitution only at nucleotide position 8344 in the t-RNA(Lys) region. The substitution was not found in 15 controls. Various degrees of the combined enzymic defects in the oxidative phosphorylation system of mitochondria were found in the MERRF patients. The defects could be explained by altered function or processing of the mutant t-RNA(Lys). This mutation in the t-RNA(Lys) is the most probable cause of MERRF.
Density-Based Clustering Validation (2014)

One of the most challenging aspects of clustering is validation, which is the objective and quantitative assessment of clustering results. A number of different relative validity criteria have been proposed for the validation of globular, clusters. Not all data, however, are composed of globular clusters. Density-based clustering algorithms seek partitions with high density areas of points (clusters, not necessarily globular) separated by low density areas, possibly containing noise objects. In these cases relative validity indices proposed for globular cluster validation may fail. In this paper we propose a relative validation index for density-based, arbitrarily shaped clusters. The index assesses clustering quality based on the relative density connection between pairs of objects. Our index is formulated on the basis of a new kernel density function, which is used to compute the density of objects and to evaluate the within- and between-cluster density connectedness of clustering results. Experiments on synthetic and real world data show the effectiveness of our approach for the evaluation and selection of clustering algorithms and their respective appropriate parameters.
Probing the force-from-lipid mechanism with synthetic polymers (2022)

Abstract A central feature of mechanotransduction is the ability of mechanosensitive channels to respond to mechanical stimuli from the surrounding lipid bilayer. Accordingly, the mechanical properties of membranes should play an important role in modulating force transmission to embedded channels, yet the nature of this relationship remains unclear for a wide class of mechanosensitive channels across prokaryotic and eukaryotic systems. Here, we use a synthetic amphiphile to modulate the membrane mechanical properties of cell-derived vesicles and probe channel activation. Using precise membrane mechanical characterization approaches that have rarely been used in conjunction with electrophysiology techniques, we directly characterize three membrane properties and the activation threshold of the E. coli mechanosensitive channel of large conductance (MscL). Our study reveals that decreases in the membrane area expansion modulus, K A , and bending rigidity, k c , correlate with increases in the pressure required to activate MscL and that this effect is reproducible with the mammalian channel, TREK-1. MD simulations demonstrate that polymer-mediated changes in interfacial tension is the best mechanism to describe these experimental results. Together, our results bolster the force-from-lipids mechanism by demonstrating the generality of the relationship between changes in specific membrane mechanical properties and the gating pressure of MscL and TREK-1. In addition, our results reveal the mechanical mechanism by which membrane amphiphiles alter the activity and sensitivity of mechanosensitive channels through changes in long-range force transmission.
dRBAC: distributed role-based access control for dynamic coalition environments (2003)

distributed role-based access control (dRBAC) is a scalable, decentralized trust-management and access-control mechanism for systems that span multiple administrative domains. dRBAC utilizes PKI identities to define trust domains, roles to define controlled activities, and role delegation across domains to represent permissions to these activities. The mapping of controlled actions to roles enables their namespaces to serve as policy roots. dRBAC distinguishes itself from previous approaches by providing three features: (1) third-party delegation of roles from outside a domain's namespace, relying upon an explicit delegation of assignment; (2) modulation of transferred permissions using scalar valued attributes associated with roles; and (3) continuous monitoring of trust relationships over long-lived interactions. The paper describes the dRBAC model and its scalable implementation using a graph approach to credential discovery and validation.
Phase locking of waveguide CO2lasers as a result of a four-wave interaction of light beams in an absorbing liquid (1988)

Radiation from two quasi-cw waveguide CO2 lasers was phase-locked by a four-wave mirror. The coefficient of coupling between the lasers was determined.
In Vitro Recombination and Inverted Terminal Repeat Binding Activities of the Mcmar1 Transposase. (2010)

International audience
Sampling of Tissues with Laser Ablation for bottom-up Proteomics: Comparison of Picosecond Infrared Laser (PIRL) and Microsecond Infrared Laser (MIRL) (2018)

The analysis of proteomes directly from tissues requires the proteins to be released from the cells and their compartments and solubilized, which usually is achieved by mechanical homogenization. It was recently shown, that sampling of tissues with the novel picosecond infrared laser (PIRL) offers higher yields of proteins with respect to the total amount and total number of individual proteins in comparison to mechanical homogenization. Furthermore, proteins obtained from tissues by homogenization with PIRL are significantly less enzymatically degraded, giving improved access to the original composition of proteoforms. The effective cold vaporization of tissue with PIRL is very soft, which is responsible for the phenomenon, that even enzymatic activities of proteins in the tissue aerosol are maintained. In contrast, the energy following irradiation of tissue with microsecond infrared laser (MIRL) pulses is not thermally and acoustically confined to the ablated volume. In this study, PIRL (1 J·cm-2) and MIRL (40-60 J·cm-2) were compared for sampling different tissue types for bottom-up proteomics. We showed that PIRL at low fluence is optimal for soft tissue and desired in scenarios were enzymatic activities of proteins must be maintained as well as were no residual tissue damage is a requirement. MIRL could be well suited for scenarios were enzymatic activities must be suppressed within the intact tissue and thermal and acoustic damage is not a concern.
Quantum Simulation and Optimization in Hot Quantum Networks (2018)

We propose and analyze a setup based on (solid-state) qubits coupled to a common multi-mode transmission line, which allows for coherent spin-spin interactions over macroscopic on-chip distances, without any ground-state cooling requirements for the data bus. Our approach allows for the realization of fast deterministic quantum gates between distant qubits, the simulation of quantum spin models with engineered (long-range) interactions, and provides a flexible architecture for the implementation of quantum approximate optimization algorithms.
Upward-Pointing Cosmic-Ray-like Events Observed with ANITA (2018)

These proceedings address a recent publication by the ANITA collaboration of four upward- pointing cosmic-ray-like events observed in the first flight of ANITA. Three of these events were consistent with stratospheric cosmic-ray air showers where the axis of propagation does not inter- sect the surface of the Earth. The fourth event was consistent with a primary particle that emerges from the surface of the ice suggesting a possible τ-lepton decay as the origin of this event. These proceedings follow-up on the modeling and testing of the hypothesis that this event was of τ neutrino origin.