gebhart/patrepeval huggingface dataset:
Location, location, location: the BRMS1 protein and melanoma progression (2012)

The metastasis suppressor, BRMS1, has been demonstrated to cause dramatic regression of metastatic lesions without blocking orthotopic tumor growth. The role of BRMS1 is well-documented for several non-melanoma malignancies, such as breast cancer, ovarian cancer and non-small-cell lung cancer. However, its role in melanoma is just beginning to be understood, with a recent article by Slipicevic et al. highlighting the levels of expression of BRMS1 in benign nevi, primary and metastatic melanoma samples. Their findings emphasize that the intracellular location of BRMS1 protein (cytoplasmic or nuclear), appears to have a significant impact upon the metastatic capacity of melanoma cells. Interestingly, this selective localization translates into a statistically significant decrease in the relapse-free period in melanoma patients, further associated with a thicker Breslow's depth of primary melanomas. However, and more importantly, this study begins to define a clearer role for BRMS1 in melanoma that is strictly dependent upon its cellular location, with nuclear expression associated with invasive and metastatic capacity and cytoplasmic expression resulting in repressive effects upon progression and metastasis. Please see related article: http://www.biomedcentral.com/1471-2407/12/73
Preclinical Pharmacology of AZD5363, an Inhibitor of AKT: Pharmacodynamics, Antitumor Activity, and Correlation of Monotherapy Activity with Genetic Background (2012)

AKT is a key node in the most frequently deregulated signaling network in human cancer. AZD5363, a novel pyrrolopyrimidine-derived compound, inhibited all AKT isoforms with a potency of 10 nmol/L or less and inhibited phosphorylation of AKT substrates in cells with a potency of approximately 0.3 to 0.8 μmol/L. AZD5363 monotherapy inhibited the proliferation of 41 of 182 solid and hematologic tumor cell lines with a potency of 3 μmol/L or less. Cell lines derived from breast cancers showed the highest frequency of sensitivity. There was a significant relationship between the presence of PIK3CA and/or PTEN mutations and sensitivity to AZD5363 and between RAS mutations and resistance. Oral dosing of AZD5363 to nude mice caused dose- and time-dependent reduction of PRAS40, GSK3β, and S6 phosphorylation in BT474c xenografts (PRAS40 phosphorylation EC(50) ~ 0.1 μmol/L total plasma exposure), reversible increases in blood glucose concentrations, and dose-dependent decreases in 2[18F]fluoro-2-deoxy-D-glucose ((18)F-FDG) uptake in U87-MG xenografts. Chronic oral dosing of AZD5363 caused dose-dependent growth inhibition of xenografts derived from various tumor types, including HER2(+) breast cancer models that are resistant to trastuzumab. AZD5363 also significantly enhanced the antitumor activity of docetaxel, lapatinib, and trastuzumab in breast cancer xenografts. It is concluded that AZD5363 is a potent inhibitor of AKT with pharmacodynamic activity in vivo, has potential to treat a range of solid and hematologic tumors as monotherapy or a combinatorial agent, and has potential for personalized medicine based on the genetic status of PIK3CA, PTEN, and RAS. AZD5363 is currently in phase I clinical trials.
Abstract 5229: Apigenin inhibits TGF-beta-induced VEGF expression in human prostate carcinoma cells via Smad2/3 and Src/FAK/Akt signaling pathways (2012)

Abstract Prostate cancer mortality is primarily attributed to development of metastases rather than primary, organ-confined disease. Solid tumors, such as prostate carcinomas, rely on establishment of vascular supply and angiogenesis for their growth and metastasis. Vascular endothelial growth factor (VEGF) is a key regulator of blood supply establishment in prostate tissue. VEGF production is regulated by two major mechanisms, hypoxia (low oxygen supply) and various cytokines. Interplay between hypoxia, cytokines, growth factors and angiogenic factors determines development and progression of cancer. In our previous studies, we demonstrated that the chemopreventive bioflavonoid apigenin (4α, 5, 7-trihydroxyflavone) inhibited hypoxia-induced elevation of VEGF production in prostate cancer cells at low oxygen conditions characteristic for solid tumors. Low oxygen (hypoxia) and transforming growth factor-β are two major factors responsible for increased VEGF secretion in prostate cancer. In metastatic prostate cancer, TGF-β serves as a promoter of prostate tumorigenesis. Activation of TGF-β receptors induces phosphorylation of its cytoplasmic effectors, receptor-regulated Sma- and Mad-related proteins (Smads). Phosphorylated Smads translocate to the nucleus and bind to DNA to regulate transcription of specific genes, notably VEGF. Blocking of TGF-β action inhibits tumor viability, tumor cell migration and metastasis in prostate cancer. Therefore, reduction of TGF-β production and activity may be a promising target for controlling tumor growth and ultimately prostate cancer metastasis. In the present studies, we investigated the inhibitory effect of apigenin on TGF-β-induced VEGF production and the mechanisms underlying this action. We found that VEGF expression was induced by TGF- β1 in highly metastatic human prostate cancer PC3-M and LNCaP C4-2B cells, and treatment with apigenin markedly decreased VEGF production. Furthermore, apigenin inhibited TGF-β1-induced phosphorylation and nuclear translocation of Smad2 and Smad3. We also found that apigenin inhibited Src, FAK and Akt phosphorylation in PC3-M and LNCaP C4-2B cells. Further experiments demonstrated that constitutively active Src reversed the inhibitory effect of apigenin on VEGF expression and Smad2/3 phosphorylation. Taken together, our results suggest that apigenin inhibits prostate carcinogenesis by modulating TGF-β-activated pathways linked to cancer progression and metastases, in particular the Smad2/3 and Src/FAK/Akt pathways. These findings provide new insights into molecular pathways targeted by apigenin, and reveal a novel molecular mechanism underlying the antiangiogenic potential of apigenin. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5229. doi:1538-7445.AM2012-5229
Brain‐derived neurotrophic factor accelerates gut motility in slow‐transit constipation (2014)

BDNF plays an important regulatory role in gut motility in STC. It was mediated by altering the intestinal innervation structure, as well as smooth muscle secondary degeneration through a mechanism involving TrkB-PLC/IP3 pathway activation.
Cardiopulmonary bypass (CPB) in the rat with a new miniaturized hollow fiber oxygenator (2008)

Aims: CPB is an essential component of cardiac surgery, with still unknown device/patient interactions. In order to evaluate the response of CPB to hemodynamic, biochemical, inflammatory, as well as thermo- pharmacodynamic interactions, a novel miniaturized oxygenator with controlled and standardized specifications has been developed together with an improved surgical central cannulation technique.
Derivatization of Pesticide-Related Acids and Phenols for Gas Chromatographic Determination (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDerivatization of Pesticide-Related Acids and Phenols for Gas Chromatographic DeterminationC. W. StanleyCite this: J. Agric. Food Chem. 1966, 14, 3, 321–323Publication Date (Print):May 1, 1966Publication History Published online1 May 2002Published inissue 1 May 1966https://pubs.acs.org/doi/10.1021/jf60145a032https://doi.org/10.1021/jf60145a032research-articleACS PublicationsRequest reuse permissionsArticle Views125Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A 51Gb/s, 320mW, PAM4 CDR with baud-rate sampling for high-speed optical interconnects (2017)

A quarter-rate 51Gb/s PAM4 CDR with decoded dual-NRZ outputs is presented for 400GbE optical transceivers. A baud-rate data-only sampling PD with zero-crossing integrating front-end is proposed to minimize the clock generation and distribution overhead, as well as improving the noise resilience under PAM4 low-SNR inputs. Measurement results show the CDR features 1.08ps RMS clock jitter, 3.4×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-9</sup> PAM4 data recovery BER, and 6.27pJ/bit power efficiency.
Data acquisition system for beam loss monitoring of HLS storage ring at NSRL (2002)

The distributed system structure of Beam Loss Monitoring System for HLS storage ring at NSRL is introduced in this paper. The frontend electronics (including flexible pulse accumulator), CAN BUS interconnection, and software are described in detail.
Heat sensitivity of Azotobacter vinelandii genetic transformation (1983)

Heating competent Azotobacter vinelandii at 37 or 42 degrees C resulted in a total loss of competence with no loss of viability. The transformation process was relatively insensitive to heating at either temperature once DNase-resistant DNA binding was nearly complete. Although competent and 42 degrees C-treated cells bound equivalent amounts of [32P]DNA in a DNase-resistant state, no donor DNA marker (nif) or radioactivity was detected in the envelope-free cell lysate of heated cells, suggesting that DNA transport across the cell envelope was a heat-sensitive event. Competence was reacquired in a 42 degrees C-treated culture after 2 h of incubation at 30 degrees C by a process which required RNA and protein syntheses. The release of a surface glycoprotein, required for competence, from cells treated at 42 degrees C occurred in an insufficient amount to account for the total loss of competence. Recovery of competence in 42 degrees C-treated cells and further transformation of competent cells were prevented by the exposure of cells to saturating amounts of transforming DNA. Further DNase-resistant DNA binding, however, still occurred, suggesting that there were two types of receptors for DNase-resistant DNA binding to competent A. vinelandii. DNase-resistant DNA binding was dependent on magnesium ions, and at least one receptor type did not discriminate against heterologous DNA.
Nonlinear and Chaotic Vibration-Based Damage Detection (2003)

The dynamics of an aeroelastic system composed of a panel forced by unsteady buffeting aerodynamic loads is investigated numerically. The focus is on detecting parametric changes in the system. The upstream end point of the panel is considered supported by a spring of variable stiffness. Changes of in the stiffness of the spring are detected by exploring the chaotic dynamics of the panel.
Lumped-parameter and finite-element-models for dynamic behavior of plane straight sided frames. (1968)

Lumped-parameter and finite-element-models are developed for the dynamic behavior of plane straight sided frames. The models do not include axial and shear deformation, but the equations of motion developed using the models allow for time varying external loading. The performance of these models is evaluated by a comparison with a standard transfer matrix method for the special case of free undamped vibration. The finite-element-model proves to be much the better model. For the first five modes, the finite-element-model with 27 degrees of freedom differs by no more than 0.4% from the 'exact values' given by the transfer matrix method. The lumped-parameter-model with 39 degrees of freedom gives errors roughly ten times as great. (Author)
Inactivation Protocols for African Swine Fever Virus in Serum and Saliva Samples (2022)

African swine fever (ASF) is a notifiable viral disease of domestic and wild suids. Despite intensive re-search efforts, the pathogenesis of the disease is still far from being understood. Analysis of biomarkers in different body fluids may supplement traditional pathogenesis studies. As reliable protocols are often es-tablished in laboratories with lower biosafety, reliable inactivation of samples is crucial. The objective of this study was to find a procedure that inactivates the virus while preserving the biomarkers for down-stream analyses. To this means, three different inactivation protocols were employed, namely Tergitol-type NP-40 (NP-40) and polyoxyethylene-p-t-octylphenol (Triton X-100), respectively, and one with 95 &amp;deg;C heating. It could be demonstrated that all samples treated with 0.5% (v/v) concentration of both deter-gents showed absence of virus infectivity. The same was true for heated samples. However, heated serum was not suitable for analyses. Next, the treatment impact on biomarker readouts was assessed. While all protocols had an impact on the detection of biomarkers, correlation was retained. Especially NP-40 could be the desired detergent for more accurate measurements while achieving efficient virus inactivation. Based on these studies, samples can be reliably inactivated for most biomarker analyses and thus broader interdisciplinary cooperation is possible.
The Assimilation of γ-Butyrolactone in Agrobacterium tumefaciens C58 Interferes with the Accumulation of the N -Acyl-Homoserine Lactone Signal (2004)

Agrobacterium tumefaciens C58 communicates using N-acyl-homoserine lactones (acyl-HSL) and contains two lactonase-encoding genes, attM and aiiB, the products of which are capable of inactivating the acyl-HSL signal. In A. tumefaciens A6, the expression of the attKLM operon is controlled by the transcriptional repressor encoded by an adjacent gene, attJ. An attJ::Tn5 mutant does not accumulate acyl-HSL because of the constitutive expression of the lactonase AttM, the activity of which inactivates acyl-HSL. In this work, the attKLM operon of A. tumefaciens C58 was shown to be involved in an assimilative pathway of gamma-butyrolactone (GBL), gamma-hydroxybutyrate (GHB), and succinate semialdehyde (SSA), in which AttM and AttL are key enzymes for GBL and GHB assimilation. The expression of the attKLM promoter was activated in the presence of GBL, GHB, and SSA. Under these conditions, A. tumefaciens C58 did not accumulate the acyl-HSL that it naturally synthesizes, and also became able to inactivate exogenous acyl-HSL signals. Therefore, in A. tumefaciens C58, the assimilative pathway of gamma-butyrolactone interferes with the acyl-HSL signaling.
Protein sumoylation with SUMO1 promoted by Pin1 in glioma stem cells augments glioblastoma malignancy (2020)

Our findings indicate that high SUMO1-modified protein sumoylation as a feature of GSCs is critical for GSC maintenance, suggesting that targeting SUMO1 sumoylation may effectively improve GBM treatment.
Heavy Ion Injection for Electric Field Generation in Tokamaks (1984)

The injection of heavy ions such as Mg+ or Ca+ into a tokamak such that they are trapped in “fat” banana orbits is proposed as a technique for creation of a radial electric field. Such a field is of interest for improving the confinement of fuel ions relative to the confinement of alpha-particle ash.
Myocardial surface Po2 - an indicator of myocardial tissue oxygenation? (1989)

The validity of myocardial surface tissue PO2 (PtO2) as a reliable indicator of transmural myocardial tissue oxygenation was studied in six anaesthetised, open chest pigs. Epicardial surface PtO2 was correlated with other variables of myocardial tissue oxygenation such as regional blood flow, coronary venous PO2, O2 saturation, PCO2 and regional myocardial lactate extraction. The study design was based on an experimental model in which the effects of a pacing induced tachycardia on tissue oxygenation of ischaemic and normally supplied myocardium were measured. Two platinum multiwire surface electrodes were placed on the epicardium, on the areas supplied by the left anterior descending coronary artery (LAD) and the left circumflex coronary artery (CX). The LAD was constricted to reduce mean surface PtO2 in the LAD area to about 50% of its baseline value. This did not affect surface PtO2 in the CX area. The reduction of surface PtO2 in the LAD area was associated with decreases in coronary venous PO2 and O2 saturation and with increases in coronary venous lactate and PCO2. Subendocardial regional blood flow and the subendocardial to subepicardial flow ratio were significantly lower than in the CX area. Increasing the heart rate by pacing (+45 beats.min-1) led to an increased degree of ischaemia as shown by fall in surface PtO2 in the LAD area to values around zero kPa, by marked increase in coronary venous lactate and PCO2, by reduction in total (-10%) and subendocardial (-40%) LAD flow and by deterioration of the subendocardial to subepicardial flow ratio. The increased degree of ischaemia was not accompanied by an increase in O2 extraction. The marked decrease in surface PtO2 occurred in spite of a slight increase in the subepicardial regional blood flow (+10%); thus the increase in O2 delivery was not sufficient to meet the increase in O2 demand. Total flow was increased by 27% in the CX area without changes in the subendocardial to subepicardial flow ratio and in the surface PtO2 values. When pacing was stopped, surface values of PtO2 in the LAD area returned to prepacing values, as did lactate extraction and coronary venous PCO2. Clear and close relationships with surface PtO2 were found for regional lactate extraction, coronary venous PCO2 and the normalised subendocardial RBF. Poor or no correlations were found for the normalised subepicardial regional blood flow, the coronary venous O2 saturation and the absolute values of subendocardial and subepicardial regional blood flow.(ABSTRACT TRUNCATED AT 400 WORDS)
ChemInform Abstract: SYNTHESIS AND ANTIARRHYTHMIC ACTIVITY OF NEW 1‐(1‐(2‐(3‐(ALKYLAMINO)‐2‐HYDROXYPROPOXY)PHENYL)VINYL)‐1H‐IMIDAZOLES AND RELATED COMPOUNDS (1985)

Abstract Synthetisiert werden die Vinylheterocyclen (IV)‐(VI), von denen (VI) antiarrhythmisch wirksam ist.
Measurement of infrared refractive indices of organic and organophosphorous compounds for optical modeling (2017)

The complex optical refractive index contains the optical constants, n(ῦ)and k(ῦ), which correspond to the dispersion and absorption of light within a medium, respectively. By obtaining the optical constants one can in principle model most optical phenomena in media and at interfaces including reflection, refraction and dispersion. We have developed improved protocols based on the use of multiple path lengths to determine the optical constants for dozens of liquids, including organic and organophosphorous compounds. Detailed description of the protocols to determine the infrared indices will be presented, along with preliminary results using the constants with their applications to optical modeling.
Antimicrobial resistance among uropathogens that cause community-acquired urinary tract infections in Antananarivo, Madagascar (2006)

The rate of amoxicillin- and trimethoprim/sulfamethoxazole-resistant Enterobacteriaceae implies that another antibiotic should be used for empirical treatment and that there is a need for new generic drugs in developing countries, especially in Madagascar.
The ENUBET Beamline (2019)

The ENUBET ERC project (2016-2021) is studying a narrow band neutrino beam where lepton production can be monitored at single particle level in an instrumented decay tunnel. This would allow to measure νμ and νe cross sections with a precision improved by about one order of magnitude compared to present results. In this proceeding we describe a first realistic design of the hadron beamline based on a dipole coupled to a pair of quadrupole triplets along with the optimisation guidelines and the results of a simulation based on G4beamline. A static focusing design, though less efficient than a horn-based solution, results several times more efficient than originally expected. It works with slow proton extractions reducing drastically pileup effects in the decay tunnel and it paves the way towards a time-tagged neutrino beam. On the other hand a horn-based transferline would ensure higher yields at the tunnel entrance. The first studies conducted at CERN to implement the synchronization between a few ms proton extraction and a horn pulse of 2-10 ms are also described.
Degraded document image binarization combining local statistics (2009)

In this paper, an efficient binarization algorithm for degraded document images is proposed. The proposed method contains two procedures: background estimation and foreground detection. The background is estimated by median filtering. The rationality is the characters can be assumed as irregular pixels. Text regions are detected by background subtraction and local variance calculation, and then local linear transform is performed to the detected text regions. Finally, a naive thresholding algorithm, ex. Otsu's method, is performed to obtain the binary result. Experimental results show that the proposed method achieves promising results.
14.3: Ujoy Technology for Personal Projection (2007)

Abstract Personal projectors are compact and affordable devices used for gaming, entertainment or photo projection. A very bright light source is at least as important as for standard front projectors. The new 50W Ujoy lamp system with 1mm arc enables efficient projection systems with a screen brightness of 200–300lm. Lower cooling requirements, the potential for battery operation and the low voltage input makes it the ideal source for this new category of projectors.
Successful Management of Severe Peripheral Tissue Ischemia after Arterial Catheterization in Micro Preemies using Humidification & Topical Nitroglycerin (2017)

Micro preemies usually undergo arterial catheterization for frequent blood pressure monitoring and blood sampling. Peripheral tissue injury associated arterial cathe terization is a welldescribed morbidity observed in neonates. Despite the potential permanent disability associated with this complication, the currently available thera peutic options remain limited. We report a unique case of a preterm infant who de veloped severe tissue ischemia after arterial catheterization of the radial artery and was successfully treated using extensive humidification and topical nitroglycerin oint ment application over an extended period (36 days) until complete clinical recovery.