gebhart/patrepeval huggingface dataset:
Spatio Temporal Influence of Isoflavonoids on Bacterial Diversity in the Soybean Rhizosphere (2014)

High bacterial density and diversity near plant roots has been attributed to rhizodeposit compounds that serve as both energy sources and signal molecules. However, it is unclear if and how specific rhizodeposit compounds influence bacterial diversity. We silenced the biosynthesis of isoflavonoids, a major component of soybean rhizodeposits, using RNA interference in hairy-root composite plants, and examined changes in rhizosphere bacteriome diversity. We used successive sonication to isolate soil fractions from different rhizosphere zones at two different time points and analyzed denaturing gradient gel electrophoresis profiles of 16S ribosomal RNA gene amplicons. Extensive diversity analysis of the resulting spatio temporal profiles of soybean bacterial communities indicated that, indeed, isoflavonoids significantly influenced soybean rhizosphere bacterial diversity. Our results also suggested a temporal gradient effect of rhizodeposit isoflavonoids on the rhizosphere. However, the hairy-root transformation process itself significantly altered rhizosphere bacterial diversity, necessitating appropriate additional controls. Gene silencing in hairy-root composite plants combined with successive sonication is a useful tool to determine the spatio temporal effect of specific rhizodeposit compounds on rhizosphere microbial communities.
The differential parameter method for multifrequency airborne resistivity mapping (1996)

Abstract Helicopter EM resistivity mapping began to be accepted as a means of geologic mapping in the late 1970s. The data were first displayed as plan maps and images. Some 10 years later, sectional resistivity displays became available using the same “pseudolayer” half-space resistivity algorithm developed by Fraser and the new centroid depth algorithm developed by Sengpiel. Known as Sengpiel resistivity sections, these resistivity/depth images proved to be popular for the display of helicopter electromagnetic (EM) data in conductive environments. A limitation of the above resistivity and depth algorithms is that the resulting Sengpiel section may imply that the depth of exploration of the EM system is substantially less than is actually the case. For example, a target at depth may be expressed in the raw data, but its appearance on the Sengpiel section may be too shallow (which is a problem with the depth algorithm), or it may not even appear at all (which is a problem with the resistivity algorithm). An algorithm has been adapted from a ground EM analytic method that yields a parameter called the differential resistivity, which is plotted at the differential depth. The technique yields the true resistivity when the half-space is homogeneous. It also tracks a dipping target with greater sensitivity and to greater depth than does the Sengpiel display method. The input parameters are the apparent resistivity and apparent depth from the pseudolayer half-space algorithm and the skin depth for the various frequencies. The output parameters are differential resistivity and differential depth, which are computed from pairs of adjacent frequencies.
On-site Management Model of Enterprise Based on Process Analysis (2009)

There are such weak aspects as logistics chaos,uneven production,high consumption and low efficiency in the current on-site management in many Chinese enterprises.By using 5S and Process Analysis,a new on-site management model is obtained,which could handle production control,materials management,equipment management,technology management,environment at scene and safety management.The new model contributes to the cost of human resources reduction by 30%,the material utility increase by 25% and overall production efficiency improvement by 45%.
A combined particle/Kalman filter for improved tracking of beam aspect targets (2003)

Track continuity is difficult to maintain when tracking beam aspect targets. The loss of Doppler discrimination allows clutter to mask the target return, making it nearly impossible to detect. In order to improve tracking performance, a combination particle/Kalman filter has been developed. The tracking filters obviate each other as appropriate. When a target enters a Doppler blind zone, the particle filter replaces the Kalman filter as the tracking algorithm until the target exits the zone. The particle filter expands over the uncertainty region so that when the target is once again visible, it can immediately resume track via re-initialization of the Kalman filter. This paper discusses the design and simulation of this algorithm and shows the resulting improvement in track continuity. We briefly discuss how our combined particle/Kalman filter approach can be used to address the problem of targets obscured in altitude return.
Totalsynthese von natürlichem α‐Tocopherol. 1. Mitteilung. Herstellung bifunktioneller, optisch aktiver Synthesebausteine für die Seitenkette mit Hilfe mikrobiologischer Umwandlungen (1979)

Total Synthesis of Natural α‐Tocopherol. I. Preparation of Bifunctional Optically Active Precursors for the Synthesis of the Side Chain by Means of Microbiological Transformations Our concept for a new total synthesis of natural α‐tocopherol includes the synthesis of a corresponding (3 R , 7 R )‐configurated C 15 side chain to be built up by using twice an optically active C 5 unit together with an achiral C 5 end part. ( S )‐3‐methyl‐γ‐butyrolactone ( 11 ) and ( S )‐2‐methyl‐γ‐butyrolactone ( 9 ) represent suitable bifunctional C 5 ‐precursors for this purpose. These two key compounds have been prepared by fermentative transformation including the enantioselective hydrogenation of the double bond of ethyl‐4, 4‐dimethoxy‐3‐methylcrotonate ( 5 ) by bakers yeast (yielding 11 after ester hydrolysis and cyclization of the fermentation product) and ( E )‐3‐(1′, 3′‐dioxolan‐2′‐yl)‐2‐buten‐1‐ol ( 8 ) by the fungus Geotrichum candidum (yielding directly 9 ).
After-fracture redundancy analysis of an aged truss bridge in Japan (2016)

In the next 20 years, more than half of the bridges in Japan would be in service for more than 50 years. These include truss bridges those are generally considered to be non-redundant and fracture critical. With ageing, these bridges are facing an increasing risk of bridge collapse under extreme conditions. Therefore, it is essential to evaluate alternate load paths on existing bridge structures to avoid bridge collapse or replacement. In this paper, after-fracture redundancy of truss bridges is investigated through a case study for a five-span continuous truss bridge. Field tests were performed, and the displacements on key sections were measured in the test. According to the experimental observations and test results, a numerical model capable of simulating the present truss bridge was built and was used to investigate the after-fracture redundancy of the target bridge. Fracture of truss members in typical locations was considered in the numerical analyses, and the ‘R’ values of both intact and damaged trusses were compared. On the basis of the numerical results, a critical member in this bridge was also determined.
Negative Magnetic Permeability in the Visible Light Region (2005)

Negative magnetic permeability of single split-ring resonators (SSRRs) is theoretically investigated in the visible light region. To describe the conduction characteristics of metal in the visible range, we develop the internal impedance formula completely. In our calculations, we determine the magnetic responses of the SSRR accurately. Based on our investigations, we also demonstrate the negative mu of the silver SSRR array in the visible light region.
Liquid Chromatography– Time-of-Flight Mass Spectrometry for Cannabinoid Profiling and Quantitation in Hemp Oil Extracts (2018)

A primary impediment to cannabinoid research is the fact that materials possessing psychoactive Δ9-tetrahydrocannabinol (THC) are considered Schedule I drugs as defined in the U.S. Controlled Substances Act. An alternative source of cannabinoids may be found in hemp oil extracts. Hemp contains a low percentage of THC by weight but relatively high amounts of nonpsychoactive cannabinoids. The liquid chromatography–time-of-flight mass spectrometry (LC–TOF-MS) method presented herein allows for the accurate, precise, and robust speciation, profiling, and quantification of cannabinoids in hemp oil extracts and commercial cannabinoid products for research and development laboratories. The method was determined to chromatographically separate 11 cannabinoids including differentiation of Δ8-tetrahdrocannabinol and THC with excellent linear dynamic range, specificity, and sensitivity.
Parental origin of the X chromosome in a patient with a Robertsonian translocation and Turner's syndrome. (1994)

We report on a proband with both a Robertsonian translocation and Turner's syndrome. Study of the parental origin of the X chromosome performed by microsatellite analysis indicates paternal origin of the X chromosome (Xpat). The occurrence of chromosome aberrations as a consequence of interchromosomal interactions is discussed.
Gas Turbine Blade Damper Optimization Methodology (2012)

The friction damping concept is widely used to reduce resonance stresses in gas turbines. A friction damper has been designed for high pressure turbine stage of a turbojet engine. The objective of this work is to find out effectiveness of the damper while minimizing resonant stresses for sixth and ninth engine order excitation of first flexure mode. This paper presents a methodology that combines three essential phases of friction damping optimization in turbo-machinery. The first phase is to develop an analytical model of blade damper system. The second phase is experimentation and model tuning necessary for response studies while the third phase is evaluating damper performance. The reduced model of blade is developed corresponding to the mode under investigation incorporating the friction damper then the simulations were carried out to arrive at an optimum design point of the damper. Bench tests were carried out in two phases. Phase-1 deals with characterization of the blade dynamically and the phase-2 deals with finding optimal normal load at which the blade resonating response is minimal for a given excitation. The test results are discussed, and are corroborated with simulated results, are in good agreement.
Effective surface passivation of Si surfaces by chemical deposition of (Al2O3)x(B2O3)1 − x thin layers (2012)

Abstract In this work chemical liquid deposition from sol–gel solutions has been used to obtain thin (Al 2 O 3 ) x (B 2 O 3 ) 1 − x dielectric films. Passivation of Si surfaces using these films has been studied. Morphological properties of thin films deposited on polished Si surfaces have been investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) technique. Their chemical composition, structural and optical features have been analysed by X‐ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and ultraviolet‐visible (UV–VIS) spectroscopy. Results, obtained in this work, show that silicon surfaces can be effectively passivated by thin dielectric layers deposited from chemical solutions. The thin dielectric layers developed in his work, can be recommended as back side passivation coating for Si‐based solar cells.
Geminal carbon‐proton spin‐spin coupling constants in ethyl, isopropyl and tert‐butyl compounds (1980)

Abstract The geminal 13 C 1 H coupling constants in ethyl, isopropyl and tert ‐butyl compounds having elements of the first row of the Periodic Table and halogens as substituents are determined by single resonance 13 C FT NMR spectroscopy. In addition, the values of directly bonded 13 C 13 C coupling constants are given. The substituent electronegativity dependence of the geminal 13 C 1 H coupling constant is discussed. It is concluded that the influence exerted by a β‐methyl substituent on the geminal 13 C 1 H coupling constant of 13C α ‐C β ‐ 1 H fragments is not constant and hence no simple additivity relation can be established for this coupling constant.
Cunning solutions to process improvement (2014)

This paper proceeds from the premise that process improvement is a key part of the mineral engineer's role, and one in which he/ she can make a significant contribution to shareholder value. It presents four tools to help with process improvement projects. Two are designed for process monitoring, to determine as soon as possible whether there has been a true change in performance: the cumulative sum (cusum) control chart plot with V-mask, and the control chart using a process model. The other two are used for process optimisation: simplex self-directing evolutionary operation (SSDEVOP), and a response surface model developed using a central composite rotatable experimental design (CCRD). These methods are robust, have a rigorous theoretical basis and are easy to implement. They also promote project continuity which increases the chances of success, particularly in fly-in/fly-out (FIFO) operations. Variants are used in other industries, but they are rarely if ever applied in mineral processing to-day for reasons which are unclear. The paper gives examples of the application of each method in concentrators which demonstrate that they have the potential to be useful additions to the mineral engineer's professional toolbox.
Lipocortin 1 mediates dexamethasone-induced growth arrest of the A549 lung adenocarcinoma cell line. (1992)

The synthetic glucocorticoid dexamethasone (1 microM to 1 pM) strongly (maximum greater than 80%) inhibits proliferation of the A549 human lung adenocarcinoma line (EC50 greater than 1 nM) and leads to the appearance, or a further increase (approximately 3-fold) in the expression on the cell surface, of the calcium and phospholipid binding protein lipocortin (annexin) 1. Both these effects, which are shared by hydrocortisone (1 microM) but not by progesterone or aldosterone (1 microM), are inhibited by the antiglucocorticoids RU38486 and RU43044 (1 microM). The nonsteroidal antiinflammatory drugs indomethacin (1 microM) and naproxen (10 microM) and human recombinant lipocortin 1 (0.05-5.0 micrograms/ml) also produce growth arrest in this cell line. During proliferation A549 cells spontaneously release prostaglandin E2 [10-20 ng (28-57 pmol) per ml per 5-day period] into the growth medium. In concentrations that cause growth-arrest, dexamethasone, indomethacin, and lipocortin 1 abolish the generation of this eicosanoid by A549 cells. Prostaglandin E2 itself (0.01-1 pM) stimulates cell growth and partially reverses (approximately 50%) the inhibition of growth caused by dexamethasone and indomethacin. Addition of the neutralizing anti-lipocortin 1 monoclonal antibody 1A (5 micrograms/ml), but not the nonneutralizing anti-lipocortin monoclonal antibody 1B, substantially reversed (greater than 80%) the inhibitory activity of dexamethasone on both growth and prostaglandin E2 synthesis. The generation of prostaglandin E2 by A549 cells seems to be an important regulator of cell proliferation in vitro and the dexamethasone-induced suppression of proliferation in this model is attributable to eicosanoid inhibition caused by lipocortin 1.
Chronic FK506 treatment reduces amyloid β and tau levels in the hippocampus and cortex of 3xTg‐AD mice (2022)

Abstract Background Alzheimer’s disease (AD) is the most common form of neurodegenerative dementia. While major histopathological hallmarks of AD are amyloid‐β (Aβ) plaques and neurofibrillary tau tangles, soluble oligomeric forms of Aβ and tau (TauO) have been identified as the most neurotoxic species involved in synaptic degeneration. Previous studies associate alterations of the phosphatase calcineurin (CaN) to AβO‐driven toxicity. We previously have shown that CaN mediates both the neurotoxic and cognitive effects of AβO and elevated CaN levels have been also shown in AD patients, suggesting a central role of CaN in AD onset and/or clinical progression. Our previous studies in mouse models demonstrated that an acute treatment with the CaN inhibitor FK506/Tacrolimus in Tg2576 acutely injected intracerebroventricularly with AβO restores memory function in these cognitively impaired animals. Moreover, we reported absence of AD in aging human chronically treated with KF506 following solid organ transplant. To deeper investigate the involvement of CaN in AD onset and progression, we used a 3xTgAD mouse model presenting both Aβ and Tau aggregates. Method 7 month‐old 3xTg‐AD mice were chronically treated for two weeks with intraperitoneal administration of either FK506 (1mg/kg) or PBS. Using immunofluorescence and western blot analyses we analyzed the levels of CaN, total Tau and Aβ in hippocampal (dentate gyrus‐DG, CA1 and CA3) and cortical areas (frontal and parietal‐occipital cortex) in our experimental groups. Result We found significantly decreased levels of CaN in DG, CA1 and CA3 and cortical areas in FK506 treated 3xTg‐AD mice as compared to 3xTg‐AD mice injected with PBS. Furthermore, performing double staining for Aβ and total Tau, FK506 treated mice displayed significant lower levels of Aβ and Tau in all the considered areas as compared to PBS‐treated 3xTg‐AD mice. These data suggest that the treatment with FK506 might trigger some mechanisms involved in the elimination of Tau and Aβ, i.e . autophagy. Conclusion Our data confirm the role of CaN as a key player in AD. The reduced key AD pathology following CaN inhibition with FK506 suggests the use of an FDA approved drug as a potential treatment with rapid clinical translation to slow the progression of the disease.
RETRACTED: The Effects of Taurocholic Acid on Biliary Damage and Liver Fibrosis Are Mediated by Calcitonin-Gene-Related Peptide Signaling (2022)

Modulation of the TC/ α-CGRP/CALCRL/PKA/ERK1/2/TGF-β1/VEGF axis may be important in the management of cholangiopathies characterized by BA accumulation.
A Tool for Modeling and Simulation of Real-Time Systems Behaviour (2007)

In several real-time applications, tasks are not only subjected to complete their deadlines, but more control of the scheduling process is required. In such situations, quality of service is interpreted in many ways (jitters, response times, etc.). In other real-time environments the objective is to optimize a performance measure while respecting release times and deadlines; other environments permit modifications of release times, but not of the deadline times, in a controlled manner. This paper focus on additional issues related on precedence constrains and variable execution times that could be considered during scheduling. In this idea, the paper presents a way of possible integration of such issues into a tool used for modelling and simulation of real-time systems behaviour.
PROTEIN BINDING OF PANCREATIC PROTEOLYTIC ENZYMES* (1962)

sought methods that would permit an easier assay of the a2-globulin trypsin inhibitor. Many studies were made which included the determination of the inhibiting activities of the a,-and a2-globulin fractions to chymotrypsin, elastase, thrombin, plasmin, and collagenase. Although interesting data were obtained, these studies did not permit the differential assay of the serum a1-and a2-globulin trypsin inhibitors unless electrophoresis was done.
Internal Proteins of the Procapsid and Mature Capsids of Herpes Simplex Virus 1 Mapped by Bubblegram Imaging (2016)

In addition to DNA, capsids of tailed bacteriophages and their distant relatives, herpesviruses, contain internal proteins. These proteins are often essential for infectivity but are difficult to locate within the virion. A novel adaptation of cryo-EM based on detecting gas bubbles generated by radiation damage was used to localize internal proteins of HSV-1, yielding insights into how capsid maturation is regulated. The scaffolding protein, which forms inner shells in the procapsid and B capsid, is exceptionally bubbling-prone. In the mature DNA-filled C capsid, a previously undetected protein was found to underlie the icosahedral vertices: this is tentatively assigned as a storage form of the viral protease. We also observed a capsid species that appears to contain substantial amounts of scaffolding protein as well as DNA, suggesting that DNA packaging and expulsion of the scaffolding protein are coupled processes.
Gene Expression Profiles during the Initial Phase of Salt Stress in Rice (2001)

Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defense-related functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.