gebhart/patrepeval huggingface dataset:
CELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM <i>WOLFIPORIA COCOS</i> (2014)

As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in ATMP production processes. Therefore, the present study investigated a novel endopeptidase used in food biotechnology for its applicability in ATMP processes where cell detachment is needed. The Prolyl-specific Peptidase (PsP) is of nonmammalian origin and considered as safe for humans. PsP was purified from the supernatant of the fungus Wolfiporia cocos. The isolation and purification resulted in an enzyme solution with 0.19 U mg -1 prolylspecific activity. By in silico analysis it was confirmed that attachment-promoting proteins can be cleaved by PsP in a similar amount than with Trypsin. Further the proteolytic activity was determined for PsP and Trypsin by using the same enzymatic assay. Detachment with both enzymes was compared for cells used in typical therapeutic production processes namely a mesenchymal stem cell line (hMSC-TERT) as a model for a cell therapeutic, Vero and MA104 cells used for viral therapeutic or vaccine production. The cell detachment experiments were performed with comparable enzyme activities (1.6 U mL -1 ). hMSC-TERT detachment was faster with PsP than with Trypsin. For Vero cells the detachment with PsP was not only faster but also more efficient. For MA104 cells the detachment rate with PsP was similar to Trypsin. For all cell types, detachment with PsP showed less influence on cell growth and metabolism compared to standard Trypsin.Thus, three cell types used in ATMP, viral therapeutics or vaccine production can be detached efficiently and gently with PsP. Therefore, PsP shows potential for cell detachment in ATMP and viral/vaccine production processes.
MP31-19 THE IMPACT OF TWENTY-FOUR HOUR URINE STUDY ON STENT EARLY ENCRUSTATION (SEE) (2018)

You have accessJournal of UrologyStone Disease: Medical & Dietary Therapy II1 Apr 2018MP31-19 THE IMPACT OF TWENTY-FOUR HOUR URINE STUDY ON STENT EARLY ENCRUSTATION (SEE) Daniel Rittenberg, William Berg, Yefim Sheynkin, Scott Herfel, and David Schulsinger Daniel RittenbergDaniel Rittenberg More articles by this author , William BergWilliam Berg More articles by this author , Yefim SheynkinYefim Sheynkin More articles by this author , Scott HerfelScott Herfel More articles by this author , and David SchulsingerDavid Schulsinger More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1048AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Stent Early Encrustation (SEE) presents a complicated technical challenge. We sought to examine and characterize differences in twenty-four hour urine (TFHU) studies between encrusters and non-encrusters. METHODS An IRB approved prospective study identified patients with SEE, defined as a calcified stent identified within 3 months of stent placement. From June 2016 to November 2017, patients with indwelling ureteral stents were evaluated for SEE upon exchange or removal and compared to patients without SEE. Demographic data and culture data were evaluated. TFHU studies were examined and compared between these two groups. RESULTS There were fifteen cases of SEE identified. The average age was 48 years old, with 53% being female. Thirteen patients screened were identified as non-encrusters, 42% of which were female, with an average age of 54 years. Non SEE patients were found to have statistically significant higher urinary concentrations of uric acid (0.729 vs 0.460 g/day, p = 0.05), sulfate (43.2 vs 20.0 mEq/day, p = 0.02), magnesium (115.1 vs 57.2 mg/day, p = 0.01), ammonium (44.6 vs 22.0 mmol/day, p = 0.02), and total creatinine (1954.9 vs 1157.2 mg/day, p = 0.03). Additionally, when compared to SEE, non SEE patients had a trend towards significance in total urine volume (2.253 vs 1.344 liters, p = 0.06) and urinary citrate (654.2 vs 374.8, p = 0.08. CONCLUSIONS Non SEE patients were found to have a significantly higher concentration of stone inhibitors. Additionally, urine volume and urinary citrate, were higher in the non SEE patients, although not statistically significant. Further investigation into concentrations of these stone inhibitors may lead to future development of ureteral stent composition to prevent SEE. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e419 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Daniel Rittenberg More articles by this author William Berg More articles by this author Yefim Sheynkin More articles by this author Scott Herfel More articles by this author David Schulsinger More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
Learning Rope Manipulation Policies Using Dense Object Descriptors Trained on Synthetic Depth Data (2020)

Robotic manipulation of deformable 1D objects such as ropes, cables, and hoses is challenging due to the lack of high-fidelity analytic models and large configuration spaces. Furthermore, learning end-to-end manipulation policies directly from images and physical interaction requires significant time on a robot and can fail to generalize across tasks. We address these challenges using interpretable deep visual representations for rope, extending recent work on dense object descriptors for robot manipulation. This facilitates the design of interpretable and transferable geometric policies built on top of the learned representations, decoupling visual reasoning and control. We present an approach that learns point-pair correspondences between initial and goal rope configurations, which implicitly encodes geometric structure, entirely in simulation from synthetic depth images. We demonstrate that the learned representation -- dense depth object descriptors (DDODs) -- can be used to manipulate a real rope into a variety of different arrangements either by learning from demonstrations or using interpretable geometric policies. In 50 trials of a knot-tying task with the ABB YuMi Robot, the system achieves a 66% knot-tying success rate from previously unseen configurations. See https://tinyurl.com/rope-learning for supplementary material and videos.
Production of VHF Plasma with Large Area Using a Ladder Shaped Electrode – VHF Plasma Production with Narrow Gap for Solar Cells (2005)

Abstract Summary: VHF H 2 and SiH 4 /H 2 plasma was produced using a ladder shaped electrode of 1 200 mm × 141 mm and the plasma parameters were measured with the Langmuir probe. It was found that uniform VHF plasma is achieved over 1 m at a frequency of 60 MHz. VHF plasma was also produced at high pressure. When the pressure was increased from 0.3 Torr to 4 Torr, the plasma density decreased at high pressure, while the electron temperature was around 10 eV at high pressure. Furthermore, Langmuir probe characteristics indicated that there exists a high amount of negative ions at high pressure. Schematic view of the ladder shaped electrode. magnified image Schematic view of the ladder shaped electrode.
Benchmarking Remote Sensing Image Processing and Analysis on the Snapdragon Processor Onboard the International Space Station (2022)

Future space missions will process and analyze imagery onboard placing greater demands on flight computing. Traditional flight hardware provides modest compute, even when compared to laptop and desktop computers. A new generation of commercial off the shelf (COTS) processors, such as Qualcomm Snapdragon, deliver significant compute in small Size Weight and Power (SWaP) and offer direct hardware acceleration in the form of Graphics Processing Units (GPU) and Digital Signal Processors (DSP). We benchmark a variety of instrument processing and analysis software (including machine learned classifiers) on a Qualcomm Snapdragon SoC currently hosted by HPE’ s Spaceborne Computer-2 (SBC-2) onboard the International Space Station.
0752 JZP-258 Dose Titration and Transition from Sodium Oxybate in a Placebo-Controlled, Double-Blind, Randomized Withdrawal Study in Adult Participants With Narcolepsy With Cataplexy (2020)

Abstract Introduction Sodium oxybate (SXB) is a standard of care for the treatment of cataplexy and excessive daytime sleepiness in narcolepsy. JZP-258 is an oxybate product candidate (at same concentration as SXB) with 92% less sodium. JZP-258 dose adjustment during titration was evaluated. Methods At study entry, participants were taking SXB only, SXB+other anticataplectics, anticataplectics other than SXB, or were cataplexy treatment-naive. JZP-258 treatment began during a 12-week, open-label optimized treatment and titration period. Participants taking SXB only or SXB+other anticataplectics transitioned to JZP-258 at the same gram-for-gram dose as SXB and titrated to an efficacious and tolerable (optimal) dose from weeks 3-12. Participants taking other anticataplectics or who were anticataplectic-naive initiated JZP-258 at 4.5 g/night and were titrated to an optimal dose at 1-1.5 g/night/week (maximum total dose, 9 g/night). A 2-week stable-dose period and 2-week, double-blind, randomized withdrawal period followed. Results During the stable-dose period, total nightly JZP-258 dose (median [range]) was higher in participants taking SXB at study entry (SXB-only, 7.5 g [4.5-9.0], n=45; SXB+other anticataplectics, 9.0 g [6.0-9.0], n=14) compared with those not taking SXB (other anticataplectics, 7.5 g [4.5-9.0], n=23; anticataplectic-naive, 7.0 g [3.0-9.0], n=67), and dose adjustments were fewer. In most (69%) participants taking SXB at study entry who entered the stable-dose period, no change in dose was required (median [range] number of adjustments was 0 ([0-8]); for those with a change in dose, most changes were within one titration step (1.5 g/night). In participants not taking SXB at study entry, the median (range) number of adjustments was 3.0 (0-7). Conclusion Most participants taking SXB at study entry transitioned to JZP-258 treatment at the same dose with retained effectiveness. Participants not previously taking SXB achieved a tolerable and efficacious dose of JZP-258 after a median of 3 adjustments. Support Jazz Pharmaceuticals
Nucleophilic Trifluoromethylation Using Trifluoromethyl Iodide. A New and Simple Alternative for the Trifluoromethylation of Aldehydes and Ketones (2001)

A novel method for nucleophilic trifluoromethylation of aldehydes and ketones, based on photoinduced reduction of trifluoromethyl iodide by tetrakis(dimethylamino)ethylene (TDAE), is presented. [reaction: see text]
Emergency exit planning and simulation environment using gamification, artificial intelligence and data analytics (2019)

In our research, we explore methods to utilize gamified building data models and data analytics. In this paper, we present a study, where an emergency exit planning and simulation platform is implemented with a commercial center gamified data model. In the study, we compare various emergency exit location options and search the critical areas for customer evacuation. Customized user profiles are used to estimate the movement capabilities of elderly and handicapped people. The feasibility of data analytics methods for analyzing the simulation results is examined. The results show that simulations based on gamification are well-suited tools emergency exit evaluations.
Comparison of various interpretation methods of the electric probe measurements in inductively coupled Ar and O2 plasmas (2014)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Min Woo Seo, Min Keun Bae, T. H. Chung; Comparison of various interpretation methods of the electric probe measurements in inductively coupled Ar and O2 plasmas. Phys. Plasmas 1 February 2014; 21 (2): 023514. https://doi.org/10.1063/1.4867350 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioPhysics of Plasmas Search Advanced Search |Citation Search
Improved method of cell placement with symmetry constraints for analog IC layout design (2006)

Recently, it is often required in high performance analog IC design that some cells are placed symmetrically to horizontal or vertical axis. Balasa et al. proposed a method of obtaining the closest placement satisfying the given symmetry constraints and the topology constraints imposed by a sequence-pair, but this method has the following defects: (1) Some cells overlap each other. (2) The closest cell placement satisfying both the symmetry and topology constraints may not be obtained. (3) How to place cells symmetrically is mentioned only for one axis and there is no explanation for plural axes. In this paper, we propose an efficient method to obtain the closest cell placement satisfying the given symmetry constraints and the topology constraints imposed by a sequence-pair using linear programming. The proposed method obtains a simple constraint graph from a sequence-pair and derives a set of linear constraint expressions from the graph. The number of linear expressions decreases by substituting the expressions for dependent variables. Then the solutions are obtained by linear programming. The effectiveness of the proposed method was shown by computational experiments.
Detektion von Typ II Endoleaks nach endovaskulärem Aortenrepair (EVAR) von Aortenaneurysmen: Vergleich zwischen MR-Angiographie mit dem Blood-Pool-Kontrastmittel Gadofosveset und der Zweiphasen CT-Ang (2011)

Ziele: Ziel dieser prospektiven Studie war die Evaluation der Wertigkeit der MR-Angiographie (MRA) mit dem Blood-Pool-Kontrastmittel (Gadofosveset) in der Detektion von Endoleaks nach EVAR im Rahmen des postoperativen Follow-ups im Vergleich zur CT-Angiographie (CTA) Methode: Es wurden 32 Patienten (mittleres 76 Jahren (64–86 Jahre)) mit EVAR eingeschlossen. Alle Patienten wurden in kurzem Abstand mittels CTA und MRA mit Gadofosveset im First-Pass und Steady-State-Phase untersucht. Die Bildanalyse erfolgte durch zwei erfahrene Radiologen mit Konsensentscheidung bzgl. Endoleaks, zu- und abführenden Arterien, Signalveränderung in T2-w und Bildqualität. Ergebnis: Die mediane Follow-up Zeit nach EVAR betrug 22 Monate (4–59 Monate). Während bei 12 Patienten in der CTA (37,5%) ein Endoleak nachweisbar war, wurden in der MRA bei 21 Patienten ein Endoleak gefunden (65,6%) von denen 6 Endoleaks durch größenwachstum klinisch relevant wurden. Die meisten Endoleaks wurden in der Steady-State-Phase entdeckt (n=14). Die Reduktion des Durchmessers war signifikant höher in der Gruppe ohne sichtbares Endoleak in der MRA (p=0,004). In der Gesamteinschätzung von Bildqualität und diagnostischer Genauigkeit wurde die CTA in 6.3% (n=2) als überlegen und in 28.1% (n=9) als gleichwertig eingeschätzt, jedoch in 65.6% (n=21) wurde die MRA als überlegen angesehen. Schlussfolgerung: Es konnte festgestellt werden, dass die MRA mit Gadofosveset, bei gleichzeitiger vermeidbarer Strahlenexposition, eine höhere Sensitivität für die Detektion von Typ-II Endoleaks als die CTA hat.
Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms (2012)

Abstract. The impact of ocean acidification and carbonation on microbial community structure was assessed during a large-scale in situ costal pelagic mesocosm study, included as part of the EPOCA 2010 Arctic campaign. The mesocosm experiment included ambient conditions (fjord) and nine mesocosms, with pCO2 range from ~145 to ~1420 μatm. Samples collected at nine time points (t-1, t1, t5, t7, t12, t14, t22, t26 to t28) in seven treatments (ambient fjord (~145), 2×~185, ~270, ~685, ~820, ~1050 μatm) were analysed for "free-living" and "particle associated" microbial community composition using 16S rRNA amplicon sequencing. This high-throughput sequencing analysis produced ~20 000 000 16S rRNA V4 reads, which comprised 7000 OTUs. The main variables structuring these communities were, sample origin (fjord or mesocosms) and the filter size fraction (free-living or particle associated). The community was significantly different between the fjord and both the control and elevated 2 mesocosms (which were not significant different) after nutrients were added to the mesocosms; suggesting that the addition of nutrients is the primary driver of the change in mesocosm community structure. The relative importance of each structuring variable depended greatly on the time at which the community was sampled in relation to the phytoplankton bloom. The size fraction was the second most important factor for community structure; separating free-living from particle-associated bacteria. When free-living and particle-associated bacteria were analysed separately at different time points, the only taxon pCO2 was found to significantly affect were the Gammaproteobacteria after nutrient addition. Finally, pCO2 treatment was found to be significantly correlated (non-linear) with 15 rare taxa, most of which increased in abundance with higher CO2.
Current status and prospects for microbubbles in ultrasound theranostics (2013)

Encapsulated microbubbles have been developed over the past two decades to provide improvements both in imaging as well as new therapeutic applications. Microbubble contrast agents are used currently for clinical imaging where increased sensitivity to blood flow is required, such as echocardiography. These compressible spheres oscillate in an acoustic field, producing nonlinear responses which can be uniquely distinguished from surrounding tissue, resulting in substantial enhancements in imaging signal-to-noise ratio. Furthermore, with sufficient acoustic energy the oscillation of microbubbles can mediate localized biological effects in tissue including the enhancement of membrane permeability or increased thermal energy deposition. Structurally, microbubbles are comprised of two principal components--an encapsulating shell and an inner gas core. This configuration enables microbubbles to be loaded with drugs or genes for additional therapeutic effect. Application of sufficient ultrasound energy can release this payload, resulting in site-specific delivery. Extensive preclinical studies illustrate that combining microbubbles and ultrasound can result in enhanced drug delivery or gene expression at spatially selective sites. Thus, microbbubles can be used for imaging, for therapy, or for both simultaneously. In this sense, microbubbles combined with acoustics may be one of the most universal theranostic tools.
Flexible Coherent Digital Transceiver for Low Power Space Missions (2006)

A flexible coherent digital transceiver architecture has been developed by the APL in order to enable mission specific performance tailoring while maintaining modularity and minimizing program-incurred cost and risk. The new transceiver architecture is based on the heritage X-band transceiver system that is currently integrated into the New Horizons spacecraft. Using this new architecture, a low power, coherent, X-band digital transceiver has been made that meets the requirements for two-way Doppler tracking. The new transceiver contributes less than 0.01 mm/s to the Doppler velocity error measured over a 60-second interval in coherent mode. Secondary power consumption is 2.8 W in the uplink-only mode of operation including the reference oscillator. Transceiver designs on the TIMED, CONTOUR, and New Horizons spacecraft were noncoherent, which required downlink telemetry in order to support two-way Doppler tracking (Jensen, 1999). The addition of a coherent capability allows this new architecture to be used on missions where carrier-only Doppler tracking is desired. This paper provides a description of the new transceiver architecture and its demonstrated performance
Integrin-like Proteins in Candida spp. and Other Microorganisms (1999)

The vertebrate integrins provide a paradigm for cell surface proteins involved in adhesion and morphogenesis. However, homologs of integrins have been found in more primitive organisms. This review will discuss the evidence for surface proteins in Candida albicans and Candida tropicalis that contain motifs reminiscent of integrins and will analyze the contributions of one of these proteins, Int1p, to adhesion, morphogenesis, and virulence. Other microorganisms thought to express integrin-like proteins will also be addressed.
Vehicle Detection and Width Estimation in Rain by Fusing Radar and Vision (2018)

While much effort has been devoted to deep learning object detection, relatively limited attention has been paid to object detection in bad weather, e.g. rain, snow or haze. In heavy rain, the raindrop on the front windshield can make it difficult to detect object from an in-car camera. The conventional way to cope with this has been to use radar as the main detection sensor. However, radar is highly susceptible to false positives. Furthermore, many entry level radar sensors only return the centroid of each detected object, rather than its size and extent. In addition, due to lack of texture input, radar cannot discriminate a vehicle from a non-vehicle object, e.g. roadside pole. This motivates us to detect vehicle by fusing radar and vision. In this paper, we first calibrate the radar and camera with respect to the ground plane. The radar detections are then projected to the camera image for target width estimation. Empirical evaluation on a large database shows that there is a natural synergy in both sensors, as the image based estimation is found to be greatly facilitated by the accuracy of the radar detection.
Turbo Codes for Deep-Space Communications (1994)

Turbo codes were recently proposed by Berrou, Glavieux, and Thitimajshima, and it has been claimed these codes achieve near-Shannon-limit error correction performance with relatively simple component codes and large interleavers. A required E(b)/N(o) of 0.7 dB was reported for a bit error rate of 10(exp -5), using a rate 1/2 turbo code. However, some important details that are necessary to reproduce these results were omitted. This article confirms the accuracy of these claims, and presents a complete description of an encoder/decoder pair that could be suitable for deep-space applications, where lower rate codes can be used. We describe a new simple method for trellis termination, analyze the effect of interleaver choice on the weight distribution of the code, and introduce the use of unequal rate component codes, which yield better performance.