gebhart/patrepeval huggingface dataset:
Fever and Cardiac Rhythm (1986)

To study the effect of fever on cardiac rhythm and conduction, we recorded 24-hour electrocardiograms in 27 young men during an uncomplicated acute febrile infection (12 patients had adenovirus, four had influenza, three had Mycoplasma pneumoniae, and eight were undefined) and after recovery. During the first 24-hour recording period, the mean axillary temperature in the whole group was 38.4 degrees C. The mean heart rate during the febrile period was 84.0 beats per minute. After recovery, it was 66.5 beats per minute. When the temperature rose by 1 degree C, the heart rate increased on the average by 8.5 beats per minute. During the febrile period, the heart rate remained high, even during sleep. The PR interval shortened significantly at a heart rate of 60 beats per minute and occasional first- or second-degree atrioventricular blocks were rarer. The QT interval shortened significantly at heart rates of 60, 80, and 100 beats per minute. Simple and complex ventricular extrasystoles were not increased by fever. In contrast, frequent supraventricular extrasystoles developed in two patients during high fever, but not during the control recording. If an acute febrile infection induces a prolongation of the atrioventricular conduction or the QT interval, or if frequent ventricular extrasystolic beats are triggered, complications must be suspected.
Micrometeoroid impact simulations using a railgun electromagnetic accelerator (1991)

A railgun electromagnetic (EM) accelerator was used to conduct a series of hypervelocity micrometeoroid impact simulations. The tests were designed to demonstrate that railguns can provide a repeatable means of accelerating particles between 10/sup -4/ and 10/sup -7/ g to hypervelocities within a high-vacuum flight chamber. Soda-lime glass beads were accelerated up to 11 km/s impacting into silicon, aluminum, quartz, and various proprietary targets. The railgun accelerators used were 1 m long, 12.7 mm wide, square-bore railguns. The inductance gradient for these guns was 0.32 mu H/m. The conductors of the gun were made of molybdenum, and the insulating sidewalls were made of either 40% glass-filled polycarbonate or black granite. The power supply for the guns was two capacitor banks in parallel. Aluminum fuses (0.254 to 2.54 mm (1 to 10 mil) thick) with various size glass beads attached to them served as the conducting plasma on vaporization. The beads were entrained in the plasma as it formed, and were accelerated with the plasma down the gun. Gun and flight range diagnostics, along with experimental setups and results for several of the experiments, are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Tension and compression in the cytoskeleton of PC-12 neurites. II: Quantitative measurements. (1988)

We assessed the mechanical properties of PC-12 neurites by applying a force with calibrated glass needles and measured resulting changes in neurite length and deflection of the needle. We observed a linear relationship between force and length change that was not affected by multiple distensions and were thus able to determine neurite spring constants and initial, nondistended, rest tensions. 81 out of 82 neurites showed positive rest tensions ranging over three orders of magnitude with most values clustering around 30-40 mu dynes. Treatment with cytochalasin D significantly reduced neurite rest tensions to an average compression equal to 14% of the former tension and spring constants to an average of 17% of resting values. Treatment with nocodazole increased neurite rest tensions to an average of 282% of resting values but produced no change in spring constant. These observations suggest a particular type of complementary force interaction underlying axonal shape; the neurite actin network under tension and neurite microtubules under compression. Thermodynamics suggests that microtubule (MT) assembly may be regulated by changes in compressive load. We tested this effect by releasing neurite attachment to a polylysine-coated surface with polyaspartate, thus shifting external compressive support onto internal elements, and measuring the relative change in MT polymerization using quantitative Western blotting. Neurons grown on polylysine or collagen without further treatment had a 1:2 ratio of soluble to polymerized tubulin. When neurites grown on polylysine were treated with 1% polyaspartate for 15-30 min, 80% of neurites retracted, shifting the soluble: polymerized tubulin ratio to 1:1. Polyaspartate treatment of cells grown on collagen, or grown on polylysine but treated with cytochalasin to reduce tension, caused neither retraction nor a change in the soluble:polymerized tubulin ratio. We suggest that the release of adhesion to the dish shifted the compressive load formerly borne by the dish onto Mts causing their partial depolymerization. Our observations are consistent with the possibility that alterations in MT compression during growth cone advance integrates MT assembly with the advance.
A phase II clinical trial of the phosphatidylserine targeting antibody, bavituximab, in combination with pembrolizumab in patients with advanced hepatocellular carcinoma. (2020)

TPS599 Background: Phosphatidylserine (PS) is an immunosuppressive lipid segregated to the inner leaflet of the plasma membrane of normal cells. PS is externalized to the outer leaflet of the plasma membrane on cells that line tumor blood vessels, tumor cells and exosomes in the tumor microenvironment creating a specific target for anticancer treatments. Bavituximab is a PS targeting antibody that binds to PS via β2 glycoprotein-1 (β2GP1), an abundant serum glycoprotein. Bavituximab can modulate the tumor immune microenvironment to promote anti-tumor immune activity. This clinical trial is evaluating the effect of combining bavituxumab and the checkpoint inhibitor pembrolizumab in patients with advanced hepatocellular carcinoma (HCC). Methods: This is a single arm phase 2 clinical trial for patients with histologically confirmed HCC which is not eligible for curative and/or loco-regional therapy. No prior systemic therapy for HCC is allowed. Patients should be &gt; 18 years, have Child-Pugh Score A, ECOG 0-1 and meet the following laboratory criteria: total bilirubin ≤ 2.0, INR ≤ 1.7; Hgb ≥ 8.5 g/dl; AST, ALT ≤5 times ULN; platelet ≥ 50,000/mm3; and albumin ≥ 2.5 g/dl. Major exclusion criteria include thromboembolic event within the last 6 months, clinically evident ascites, GI bleeding within 2 months, uncontrolled hypertension, HIV and autoimmune disease (except DM1, childhood allergies and thyroid abnormalities if adequately controlled. Dosing is as follows: Bavituximab: 3 mg/kg IV weekly, pembrolizumab: 200 mg IV every 3 weeks. The primary objective is to determine the overall response rate (ORR) of therapy. Secondary objectives are to determine the safety, tolerability and other measures of clinical efficacy (overall survival, 6 month PFS, and duration of response). Tumor and blood samples are being collected to assess for pharmacodynamic biomarkers. A minimax two-stage method will be used to analyze ORR after the first 15 patients are accrued. If 3 or more of the first 15 patients have either a complete or partial response, 13 additional patients will be enrolled. ORR will be analyzed by RECIST 1.1 criteria. Clinical trial information: NCT03519997.
논 생태계 내 유기농법 재배 지역과 관행농법 재배 지역의 저서무척추동물군집의 특성 (2013)

최근 사회의 발전과 더불어 소비자들의 요구가 다양화되고, 환경에 대한 관심이 증가하면서 생산성 증대에서 환경 보전에 기여하는 농업으로의 전환의 필요성이 커지게 되었다. 유기농법과 관행농법과 같은 재배 방식의 차이는 생물 서식지에 다양한 영향을 끼치기 때문에 실제 유기농업이 생물에게 미치는 영향을 확인하기 위해서 두 농법에 따른 생물다양성 차이를 비교할 필요가 있다. 본 연구는 유기농법 지역과 관행농법 지역의 저서무척추동물의 군집 특성을 비교하기 위해 수행되었다. 조사 기간은 2009년부터 2011년까지였고 11개 지역을 조사 대상지로 하였다. 영농방법에 따라 개체수, 종수, 종구성 특성을 비교하였다. 저서무척추동물은 유기농법 지역에서 훨씬 많은 개체수와 종수가 기록되었으며, 종수와 개체수의 범위도 관행농법 지역에 비해 월등히 높은 것을 확인 할 수 있었다. 특히, 산림 지역으로 둘러싸인 지역에서 유기농법으로 재배할 경우 다른 경관적 요소로 둘러싸인 지역보다 저서무척추동물의 종수 및 개체수 증가에 더 큰 영향을 미치는 것을 확인 할 수 있었다. 종다양성을 유지 또는 회복하기 위해서 농약과 같은 화학물질의 사용을 제한해야 하며, 특히, 산간 지역의 논생태계에서 유기농법으로 벼를 재배할 경우 저서무척추동물의 종다양성이 크게 높아질 것으로 기대된다.
Cholesteric Gratings Doped with a Dichroic Dye (2001)

We report the results obtained from the studies of cholesteric gratings doped with a dichroic guest-host dye, G206. First, a dye-doped planar cholesteric texture was switched to a fingerprint texture. Using the pump (Ar + laser)–probe (He–Ne laser) technique, we then studied the diffraction characteristics of the Raman-Nath cholesteric grating. The results showed that the diffraction efficiencies of the first- and second-order diffracted beams decreased and increased, respectively, with increasing power of the pump beam, while those of the zeroth order remained unchanged. In addition, the diffracted beams can be steered using the pump beam. We propose a model based on the thermal effect resulting from the dye absorption of the pump beam. It explains the experimental results qualitatively well.
Macrocyclic Receptor Exhibiting Unprecedented Selectivity for Light Lanthanides (2009)

We report a new macrocyclic ligand, N,N'-bis[(6-carboxy-2-pyridil)methyl]-4,13-diaza-18-crown-6 (H(2)bp18c6), designed for complexation of lanthanide ions in aqueous solution. Potentiometric measurements evidence an unprecedented selectivity of bp18c6 for the large Ln(III) ions. Among the different Ln(III) ions, La(III) and Ce(III) show the highest log K(ML) values, with a dramatic drop of the stability observed from Ce(III) to Lu(III) as the ionic radius of the Ln(III) ions decreases (log K(CeL) - log K(LuL) = 6.9). The X-ray crystal structures of the Gd(III) and Yb(III) complexes show that the metal ion is directly bound to the 10 donor atoms of the bp18c6 ligand. The structure of the complexes in solution has been investigated by (1)H and (13)C NMR spectroscopy, as well as by theoretical calculations performed at the DFT (B3LYP) level. Our results indicate that a conformational change occurs around the middle of the lanthanide series: for the larger Ln(III) ions the most stable conformation is Delta(deltalambdadelta)(deltalambdadelta), while for the smallest Ln(III) ions (Gd-Lu) our calculations predict the Delta(lambdadeltalambda)(lambdadeltalambda) form being the most stable one. This structural change was confirmed by the analysis of the Ce(III)-, Pr(III)-, and Yb(III)-induced paramagnetic (1)H shifts. The selectivity that bp18c6 shows for the large Ln(III) ions can be attributed to a better fit between the light Ln(III) ions and the relatively large crown fragment of the ligand. Indeed, our DFT calculations indicate that the interaction between the Ln(III) ion and several donor atoms of the crown moiety is weakened as the ionic radius of the metal ion decreases.
ChimeRScope: a novel alignment-free algorithm for fusion gene prediction using paired-end short reads (2016)

Fusion genes are those that result from the fusion of two or more genes, and they are typically generated due to the perturbations in the genome structure in cancer cells. In turn, fusion genes can contribute to tumor formation and progression by promoting the expression of an oncogene, deregulation of a tumor-suppressor, or producing much more active abnormal proteins. More importantly, oncogenic fusion genes are specifically expressed in the tumor cells, which provide enormous diagnostic and therapeutic advantages for cancer treatment. With the development of next-generation sequencing (NGS) technology, RNA-Seq becomes increasingly popular for transcriptomic study because of its high sensitivity and the capability of detecting novel transcripts including fusion genes. To date, many fusion gene detection tools have been developed, most of which attempt to find reliable alignment evidence for chimeric transcripts from RNA-Seq data. It is well accepted that the alignment quality of sequencing reads against the reference genome is often limited when significant differences in the genomes exist, which is the case with cancer genomes that contain many genomic perturbations and structural variations. Hence, regions where fusion genes occur in the cancer genome tend to be largely different from those in the reference genome, which prevents the alignment-based fusion gene detection methods from achieving good accuracies. We developed a tool called ChimeRScope. ChimeRScope, being an alignment-free method, bypasses the sequence alignment step by assessing the gene fingerprint profiles (in the form of k-mers) from RNA-Seq paired-end reads for fusion gene prediction (Chapter Two). We also optimized the data structure and ChimeRScope algorithms, in order to overcome the common limitations (memory-utilization, low accuracies) that are commonly seen in alignment-free methods (Chapter Two). Results on simulated datasets, previously studied cancer RNA-Seq datasets, and experimental validations on in-house datasets have shown that ChimeRScope consistently performed better than other popular alignment-based methods irrespective of the read length and depth of sequencing coverage (Chapter Three). ChimeRScope also generates graphical outputs for illustrations of the fusion patterns. Lastly, we also developed downloadable software for ChimeRScope and implemented an online data analysis server using the Galaxy platform (Chapter Four). ChimeRScope is available at https://github.com/ChimeRScope/ChimeRScope/.
[Targeting hTERT gene influenced the viability on hMSCs by small hairpin RNA]. (2006)

The results suggest that shRNA plasmid directed against hTERT can not influence the viability on hMSCs. The treatment with RNA interference may be a safety strategy for gene therapy in vivo.
Localization of Sequences Required in cis for Yeast Ty1 Element Transposition near the Long Terminal Repeats: Analysis of Mini-Ty1 Elements (1990)

In order to identify and characterize sequences within Ty1 elements which are required in cis for transposition, a series of mini-Ty1 plasmids were constructed and tested for transposition. Mini-Ty1s are deletion mutants of the Ty1-H3 element; Ty1 gene products required for transposition are supplied in trans from a helper Ty1 which has intact open reading frames but lacks a 3' long terminal repeat (LTR) and therefore cannot transpose itself. Up to 5 kilobase pairs of internal sequences of the 6-kilobase-pair-long Ty1 element can be deleted without a significant effect on transposition. The smallest mini-Ty1 element capable of transposition contains the 3' LTR and the transcribed portion of the 5' LTR, 285 base pairs (bp) of internal sequence 3' to the 5' LTR, and 23 bp of internal sequence 5' to the 3' LTR. We conclude that Ty1-encoded proteins can act in trans and that cis-acting sequences in Ty1-H3 are all within or near the LTRs. Further deletion of the 285-bp internal sequence adjacent to the 5' LTR significantly reduced transposition frequency, and the mini-Ty1 RNA produced failed to be packaged into the viruslike particles efficiently. Surprisingly, several nonhomologous cellular mRNAs were also associated with viruslike particles.
Sarcopenia in patients undergoing maintenance hemodialysis: incidence rate, risk factors and its effect on survival risk (2016)

The incidence of sarcopenia in MHD patients was high and increased gradually with age. Dialysis duration, diabetes, serum phosphorus level and malnutrition predisposed the patients to sarcopenia. One-year follow-up found that the mortality risk of sarcopenic patients was higher than that of non-sarcopenic patients.
Polycationic β-Cyclodextrin “Click Clusters”:  Monodisperse and Versatile Scaffolds for Nucleic Acid Delivery (2008)

Herein, a novel series of multivalent polycationic beta-cyclodextrin "click clusters" with discrete molecular weight have been synthesized, characterized, and examined as therapeutic pDNA carriers. The materials were creatively designed based on a beta-cyclodextrin core to impart a biocompatible multivalent architecture and oligoethyleneamine arms to facilitate pDNA binding, encapsulation, and cellular uptake. An acetylated-per-azido-beta-cyclodextrin (4) was reacted with series of alkyne dendrons (7a-e) (containing one to five ethyleneamine units) using copper-catalyzed 1,3-dipolar cycloaddition, to form a series of click clusters (9a-e) bearing 1,2,3-triazole linkers. Gel electrophoresis experiments, dynamic light scattering, and transmission electron microscopy revealed that the macromolecules bind and compact pDNA into spherical nanoparticles in the size range of 80-130 nm. The polycations protect pDNA against nuclease degradation, where structures 9c, 9d, and 9e did not allow pDNA degradation in the presence of serum for up to 48 h. The cellular uptake profiles were evaluated in Opti-MEM and demonstrate that all the click clusters efficiently deliver Cy5-labeled pDNA into HeLa and H9c2 (2-1) cells, and compounds 9d and 9e yielded efficacy similar to that of the positive controls, Jet-PEI and Superfect. Furthermore, the luciferase gene delivery experiments revealed that the level of reporter gene expression increased with an increase in oligoethyleneamine number within the cluster arms. The cytotoxicity profiles of these materials were evaluated by protein, MTT, and LDH assays, which demonstrate that all the click clusters remain nontoxic within the expected dosage range while the positive controls, Jet PEI and Superfect, were highly cytotoxic. In particular, 9d and 9e were the most effective and promising polycationic vehicles to be further optimized for future systemic delivery experiments.
Microprocessors for the new millennium: Challenges, opportunities, and new frontiers (2002)

As designs become more complex, technology scaling more difficult, and power issues more pressing, "business as usual" no longer suffices, if the industry is to continue its long-standing tradition of microprocessor innovation. To provide means for system performance advancements and power management, there must be focus on all aspects of the computing platform-architecture, micro-architecture, bus memory, and I/O performance-much more than in the past. Multithreading and multi-core computer micro-architectures will increase both general-purpose and networking processor MIPS. Transaction-focused server processors will benefit from large on-die caches. Special-purpose architectures and circuit techniques will be required to deliver performance with higher efficiency. Future microprocessors will evolve as integration of DSP capabilities becomes imperative to enable such applications as media-rich communications, computer vision, and speech recognition. These advances in processing natural data will lead to a change in the computing paradigm from today's data-based, machine-based computing to tomorrow's knowledge-based, human-based computing. As the Internet becomes more integral to businesses and consumers, there will be new uses for and users of microprocessors. All this can be accomplished only with wired and wireless high-bandwidth Internet connectivity, driven by high-performance computer servers to fulfil the demand of computing in the Internet economy.
NONLINEAR PHENOMENA IN LASER-MATERIAL INTERACTIONS (2005)

For decades, the majority of laser-materials processing was done with lasers that employed classical (or linear) light-matter interactions. Such interactions are well understood, readily modeled, and are used for a very wide variety of laser-materials processing. In the past decade or so, however, high-power, short pulse lasers have become commercially available that can initiate several non-classical (or non-linear) light-matter interactions in materials. With such lasers the incident radiation intensity can exceeds one or more critical values, which can initiate alternative radiation absorption mechanisms that can substantially alter the fundamental radiation absorption - hence energy distribution - in a material. Such phenomena, in turn, can substantially alter the temperature history, heat transfer, thermal properties, and behavior of the material interacting with the radiation, resulting in exciting new opportunities for both basic research and practical applications. This article highlights several non-linear phenomena that can become important during short-pulse high-power laser material interactions: avalanche ionization, saturable absorption, multiphoton absorption, the optical Kerr effect, and plasma defocusing. From an applied perspective, the advantages of using non-linear laser-material interactions for materials processing are substantial, and include negligible thermal damage to the workpiece, an extremely wide range of material processing capability, and sub-micron and volumetric feature capabilities. In terms of research, non-linear laser-material interactions represent a fertile area with considerable research opportunities to characterize and clarify the nature of these interactions, while also developing innovative new ways in which to employ these mechanisms for novel processing capabilities. Applications for the understanding and deployment of non-linear laser-material interactions using high power lasers include precision laser micromaching, biological applications, diagnostic and assay capabilities, and development of unique thin-film materials.
Link-state update policies for all-optical DWDM transport networks (2004)

It has been demonstrated that advertising the availability of wavelength and converter resources in all-optical DWDM transport networks with sparse wavelength conversion capabilities can drastically improve the blocking performance of these networks thus achieving better usage of the network resources. An important aspect with consequences in network performance is when to originate the wavelength-availability and converter-availability link state advertisements (LSAs). Frequent link-state advertisements could overwhelm the network control plane with many update messages that need to be processed. On the other hand, delaying these advertisements could result in inaccurate link-state information thus degrading the network blocking performance. In this paper, we propose two different link-state update policies to advertise the availability of wavelength and converter resource in all-optical DWDM transport networks with sparse wavelength conversion capabilities. The first one is very simple and serves as our base policy for comparison. The second policy, which is very efficient in handling the dynamic nature of all-optical DWDM networks with different degrees of wavelength conversion, reduces the number of LSAs considerably while maintaining the call blocking probability.
Primary Structure of theHerpesvirus AtelesGenome (2000)

Herpesvirus ateles is an agent indigenous to spider monkeys (Ateles spp.) and causes fulminant lymphomas in various New World primates. Structural and genetic relatedness led to the classification of this virus as a member of the genus Rhadinovirus. It is most closely related to Herpesvirus saimiri. The 108,409-bp light DNA segment of the herpesvirus ateles strain 73 genome has two genes for U-RNA-like transcripts and 73 open reading frames, of which at least 6 show significant homologies to cellular genes (encoding complement control proteins, apoptosis-regulatory proteins, D-type cyclins, interleukin-8 receptors, and enzymes involved in nucleotide metabolism). The left terminal region of the light DNA segment bears the putative rhadinovirus oncogene tio.
Small scale DNA preps for Neurospora crassa (1993)

Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suitable for Southern analysis or PCR. Starting with fresh mycelium, 20 to 40 samples can be processed in approximately two hours. Better yields (about 5 micrograms) may be obtained by suspending approximately 100 microliters of ground lyophilized mycelium in 500 microliters of isolation buffer and following the protocol starting from step 4. "Spin" refers to centrifugation of samples at 14,000 rpm in a microcentrifuge.