gebhart/patrepeval huggingface dataset:
Vascular-specific expression of the bean GRP 1.8 gene is negatively regulated. (1991)

In French bean, the glycine-rich cell wall protein GRP 1.8 is specifically synthesized in the vascular tissue. To identify cis-acting sequences required for cell type-specific synthesis of GRP 1.8, expression patterns of fusion gene constructs were analyzed in transgenic tobacco. In these constructs, the uidA (beta-glucuronidase) gene was placed under control of 5' upstream deletions as well as internal deletions of the GRP 1.8 promoter. Four different cis-acting regulatory regions, SE1 and SE2 (stem elements), a negative regulatory element, and a root-specific element, were found to control the tissue-specific expression. Deletion of the negative regulatory element resulted in expression of the uidA gene in cell types other than vascular cells. The SE1 region was essential for expression in several cell types in the absence of further upstream regulatory sequences. Full-length promoters having insertions between the negative regulatory element and SE1 strongly expressed the gene in nonvascular cell types in stems and leaves. Thus, vascular-specific expression of the GRP 1.8 promoter is controlled by a complex set of positive and negative interactions between cis-acting regulatory regions. The disturbance of these interactions results in expression in additional cell types.
MECHANICAL FAULT DIAGNOSIS BASED ON ONE-CLASS CLASSIFICATION AND ITS APPLICATION (2008)

In order to solve the problem of insufficient fault samples in intelligent monitoring and diagnosis for machinery,a new method of one-class classification of mechanical fault-support vector data description was proposed in which the one-class classifier can be set up by using only one kind of target sample without knowing other outlier samples. In this way,the outlier samples can be distinguished from the target samples. The learning sample set can be built up by extracting the vibration signal features of the machinery with the normal state so that the classifier model can also be established. This model can be used to identify the running states of the equipment and diagnose the faults of it in the future. The method has been applied to the fault diagnosis of the fan in the cement plant,and the satisfactory results are obtained.
Biomechanisms of Comorbidity: Reviewing Integrative Analyses of Multi-omics Datasets and Electronic Health Records (2016)

This review highlighted the growing methods for identifying the molecular mechanisms underpinning comorbidities that leverage multiscale molecular information and patterns from electronic health records. The survey unveiled that intergenic polymorphisms have been overlooked for similarity imputation compared to their intragenic counterparts, offering new opportunities to bridge the mechanistic and similarity gaps of comorbidity.
Nonlinear control of semi-active suspension systems: A Quasi-Linear Control approach (2016)

This paper treats the control design for semi-active suspension systems using the recently-developed theory of Quasi-Linear Control (QLC). The use of QLC facilitates handling actuator constraints in semi-active suspensions and the design of nonlinear output feedback laws. Our approach consists of three steps. First, a semi-active suspension control law is defined as a polynomial function of the velocity difference between the sprung and unsprung masses. Then, QLC is employed to stochastically linearize the polynomial function within the closed-loop system. Finally, using the linearized system, a constrained optimization problem is formulated and solved to generate the coefficients of the polynomial function. This approach leads to a nonlinear feedback control law, which explicitly takes into account the actuator constraints. Numerical examples are presented to demonstrate the efficacy of this approach as compared with clipped linear quadratic Gaussian control, which is an existing approach used in the literature.
Cavitation pressure in water (2006)

We investigate the limiting mechanical tension (negative pressure) that liquid water can sustain before cavitation occurs. The temperature dependence of this quantity is of special interest for water, where it can be used as a probe of a postulated anomaly of its equation of state. After a brief review of previous experiments on cavitation, we describe our method which consists in focusing a high amplitude sound wave in the bulk liquid, away from any walls. We obtain highly reproducible results, allowing us to study in detail the statistics of cavitation, and to give an accurate definition of the cavitation threshold. Two independent pressure calibrations are performed. The cavitation pressure is found to increase monotonically from -26 MPa at 0 degrees C to -17 MPa at 80 degrees C. While these values lie among the most negative pressures reported in water, they are still far away from the cavitation pressure expected theoretically and reached in the experiment by Angell and his group [Zheng, Science 254, 829 (1991)] (around -120 MPa at 40 degrees C). Possible reasons for this discrepancy are considered.
Development of a new real-time PCR system for simultaneous detection of bacteria and fungi in pathological samples. (2015)

A novel system for simultaneous detection of pathogenic bacteria and fungi in pathological samples was developed using a real-time polymerase chain reaction (PCR) system. This system, designated the "multi-microbial real-time PCR", has the potential to simultaneously detect 68 bacterial and 9 fungal species in a 96-well plate format. All probe-primer sets were designed to produce amplicons smaller than 210 bp using formalin-fixed paraffin-embedded samples as input. The specificity and sensitivity of each probe-primer set were tested against DNA extracted from pure cultures of specific pathogens. The multi-microbial real-time PCR system revealed profiles of microorganism infection in lung samples collected at autopsy from 10 patients with acquired immunodeficiency syndrome. Staphylococcus aureus was the most common microbe detected (n=8), but with low copy numbers. High copy numbers of Pseudomonas aeruginosa were detected in the lung samples with abscess (n=6). Enterococcus faecium (n=6), Elizabethkingia meningoseptica (n=4), and Candida albicans (n=4) were also frequently detected. In addition, a latent infection of Mycobacterium tuberculosis was detected in one case of pneumonia. In conclusion, this multi-microbial real-time PCR system can be useful for detecting bacteria and fungi in pathological specimens from patients with uncertain diagnoses.
Data and Reasoning Fabric Minimum Viable Product (2021)

View Video Presentation: https://doi.org/10.2514/6.2021-2386.vid A Data & Reasoning Fabric (DRF) is envisioned to enable the full potential of air mobility by providing all data and reasoning where they are needed. The DRF provides a marketplace based on an open foundational ecosystem of data and reasoning exchange between the many systems that must seamlessly interplay to manage the complex and dense airspace operations required to achieve advanced air mobility goals. The DRF marketplace is decentralized and will not be owned by any single party. To deliver reference designs and development environments that catalyze broad private and public sector buy-in and self-sustaining development of DRF and associated standards, DRF activities will collaboratively test these technologies, open standards and architectures, and an integrated framework with end-users. The DRF activity is developing a Minimum Viable Product (MVP) called the DRF Accelerator which will enable the rapid deployment of candidate data and reasoning services to test and evaluate the DRF core system functionality. The DRF Project is engaging with government and industry end-users and stakeholders, to: (1) assess the technical feasibility of the DRF Accelerator; (2) assess the likelihood of adoption, through shared test and evaluation; and (3) identify what data and services can be shared across DRF.
pH-Responsive Oligodeoxynucleotide (ODN)−Poly(Ethylene Glycol) Conjugate through Acid-Labile β-Thiopropionate Linkage:  Preparation and Polyion Complex Micelle Formation (2003)

An oligodeoxynucleotide (ODN) conjugated to poly(ethylene glycol) (PEG) through a pH-responsive ester linkage (PEG-ODN conjugate) was successfully synthesized by the Michael reaction of 3'-thiol-modified ODN with a heterobifunctional PEG bearing an acetal group at the alpha-end and an acrylate group at the omega-end (acetal-PEG-acrylate), aimed at the development of a novel ODN delivery system. The prepared PEG-ODN conjugate and linear-poy(ethyleneimine) (L-PEI) spontaneously associated to form a polyion complex (PIC) micelle whose diameter and polydispersity index micro(2)/Gamma(2)) were 102.5 nm and 0.096 as determined by DLS measurements, respectively. Both the PEG-ODN conjugate and PIC micelle showed cleavage of the ester linkage at the endosomal pH (=5.5), suggesting that the PIC micelle is anticipated to release the ODN in the intracellular compartment. Furthermore, the PEG-ODN conjugate in the PIC micelle was stable against deoxyribonuclase (DNase I) digestion and has no interaction with the serum component because of the steric stabilization of the highly dense PEG corona surrounding the PIC core. These characteristics of the PIC micelles entrapping the PEG-ODN conjugate are promising for their utility as a novel ODN delivery system.
Identification of a cartilage-specific promoter within intron 2 of the chick alpha 2(I) collagen gene. (1990)

Chick cartilages contain type I collagen mRNAs but do not synthesize type I collagen. The 5' end of the mRNA derived from the alpha 2 type I collagen gene (alpha 2(I] in cartilage differs from the 5' end of the mRNA in cells and tissues that actively synthesize alpha 2(I) collagen. This difference in mRNA structure results from the use of a cartilage-specific transcription start site within intron 2 of the alpha 2(I) collagen gene. The use of the cartilage transcription start site replaces exons 1 and 2 with a 96-base exon contained within intron 2. The resulting transcripts contain several small open reading frames, all of which appear out of frame with the collagen coding sequence. The cartilage form of the mRNA no longer encodes alpha 2(I) collagen, thus explaining the absence of alpha 2(I) collagen in cartilage. Transcription of the alpha 2(I) collagen gene initiates at the previously described (bone/tendon) promoter in prechondrogenic limb mesenchyme, which synthesizes alpha 2(I) collagen. Thus, the cessation of alpha 2(I) collagen synthesis which occurs during differentiation of prechondrogenic mesenchymal cells into chondrocytes apparently results from the switch in promoter utilization from the bone/tendon promoter to the cartilage promoter.
Canthaxanthin Biofabrication, Loading in Green Phospholipid Vesicles and Evaluation of In Vitro Protection of Cells and Promotion of Their Monolayer Regeneration (2022)

In the present study, canthaxanthin was produced by biofermentation from Dietzia natronolimnaea HS-1 (D. natronolimnaea) and was loaded in phospholipid vesicles prepared with natural component using an easy and low dissipative method. Indeed, glycerosomes, hyalurosomes, and glycerohyalurosomes were prepared by direct hydration of both phosphatidylcholine and the biotechnological canthaxanthin, avoiding the use of organic solvents. Vesicles were sized from 63 nm to 87 nm and highly negatively charged. They entrapped a high number of the biomolecules and were stable on storage. Canthaxanthin-loaded vesicles incubated with fibroblasts did not affect their viability, proving to be highly biocompatible and capable of inhibiting the death of fibroblasts stressed with hydrogen peroxide. They reduced the nitric oxide expression in macrophages treated with lipopolysaccharides. Moreover, they favoured the cell migration in an in vitro lesion model. Results confirmed the health-promoting potential of canthaxanthin in skin cells, which is potentiated by its suitable loading in phospholipid vesicles, thus suggesting the possible use of these natural bioformulations in both skin protection and regeneration, thanks to the potent antioxidant, anti-inflammatory and antiageing effects of canthaxanthin.
UTBiome: citizen science and campus community engagement (2015)

The University of Texas main campus is located at the heart of the city of Austin. The university campus can be used as “living” laboratory to engage students, faculty, and the public in general, in science. The UTBiome (aka “Mapping the UTBiome”) project, is a cross-disciplinary educational, research and outreach effort to (1) engage the community in the collection and analysis of environmental samples from campus environment, and (2) to create an open access web-based mapping platform to disseminate the environmental and microbiological data obtained. In this project, both indoor and outdoor environments are being interrogated to answer diverse scientific questions. Over 250 citizens from the university community have been involved to date in the collection of biological samples and environment metadata associated with key water quality metrics including temperature, nitrate, phosphate, dissolved oxygen, alkalinity, pH, turbidity and fecal indicator bacteria. A data management system was designed and implemented for both, ‘online’ data input through student’s mobile phones and to facilitate sample tracking. In order to fully engage the students and the UT community, we envisioned an interactive and innovative workflow, involving technology, automation, and social media. The interactive mapping platform we have created allow users within and outside the university to access and download the results and associated environmental data by simply clicking on the interactive map. The comprehensive UTBiome map created in ArcGIS can be found in this website http://crwr-utbiome.austin.utexas.edu/utb_webapp/utbiome.html (or just http://tinyurl.com/UTBiomeMap). The online platform design offers access to the environmental information, providing resources for students, faculty and the general public to learn more about the environment that surrounds us.
Density Functional Study of π Facial Selectivity in Diels−Alder Reactions (2000)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTDensity Functional Study of π Facial Selectivity in Diels−Alder ReactionsChristian Bachmann, Norbert Böker, Martine Mondon, and Jean-Pierre GessonView Author Information Laboratoire de Catalyse en Chimie Organique (UMR 6503) and Laboratoire de Synthèse et Réactivité des Substances Naturelles (UMR 6514), 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France [email protected]Cite this: J. Org. Chem. 2000, 65, 23, 8089–8092Publication Date (Web):October 21, 2000Publication History Received18 April 2000Published online21 October 2000Published inissue 1 November 2000https://pubs.acs.org/doi/10.1021/jo000594ohttps://doi.org/10.1021/jo000594obrief-reportACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views135Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Chemical calculations,Cluster chemistry,Interaction energies,Oxygen,Selectivity Get e-Alerts
Clinical Response Predictors in a Double-Blind, Placebo-Controlled Trial of Fluoxetine for Geriatric Major Depression (1995)

Although potentially useful baseline characteristics were initially identified as response predictors, conservative statistical methods failed to confirm any significant predictors of differential responses between fluoxetine and placebo in this double-blind, placebo-controlled trial. These results suggest that response predictor analyses require confirmation before conclusions can be generalized.
THE EFFECT OF LOW DOSE HEPARIN ON THE FEEDBACK ACTIVATION OF FACTOR VIII IN VIVO (1987)

Heparin is a well established drug in the treatment and prophylaxis of thrombosis. However, the in vitro methods for thecontrol of heparin therapy (aPTT, anti-Xa, anti-II) often are not sufficiently sensitive to monitor low-dose heparin therapy. We used an assay that is based upon the in vivo activation of factor VIII at the site of skin punction: the factor VIII activity in blood samples taken from a capillary wound increases with time Hurlet-Birk Jenssen et al. Path. Biol. 24, 6 (1976). Assay: 50 μl blood samples are taken 30, 60 and 90 sec after capillary puncture. The samples are diluted with 950μl, 10 mM sodium citrate, Mich, buffer and centrifugated(3 min, 4 °C). In the diluted samples the factor VIII activity is determined. In healthy volunteers the increase in factor VIII activity in the successive samples is fairly constant (1.4 - 1.8 fold) in spite of distinct interindividual differences in the factor VIII level. In patients receiving continuous intravenous heparin (20000 IE/24 h) and in a hemophiliaA patient there was no increase in factor VIII activity with the time. In individuals receiving 5000 IE heparin subcutaneously the increase in factor VIII activity was dependent upon the time interval between heparin injection and sampling. There was almost no influence of heparin on the 30 s samples but the 90 and 150 sec. samples showed distinctly prolonged clotting times at sampling points from 2 to 4 hours after heparin administration. It was not possible to detect the heparin effectby an automated anti-IIa assay or in a prothrombin time assay because the heparinlevels were below the detection limit of the tests. These results suggest that low-dose heparin therapy has a definite influence on the feedback activation of factor VIII even in concentrations that are not detectable with the usual heparin tests.
Web-page classification through summarization (2004)

Web-page classification is much more difficult than pure-text classification due to a large variety of noisy information embedded in Web pages. In this paper, we propose a new Web-page classification algorithm based on Web summarization for improving the accuracy. We first give empirical evidence that ideal Web-page summaries generated by human editors can indeed improve the performance of Web-page classification algorithms. We then propose a new Web summarization-based classification algorithm and evaluate it along with several other state-of-the-art text summarization algorithms on the LookSmart Web directory. Experimental results show that our proposed summarization-based classification algorithm achieves an approximately 8.8% improvement as compared to pure-text-based classification algorithm. We further introduce an ensemble classifier using the improved summarization algorithm and show that it achieves about 12.9% improvement over pure-text based methods.
Fetal anatomy of the upper pharyngeal muscles with special reference to the nerve supply: is it an enteric plexus or simply an intramuscular nerve? (2013)

We examined pharyngeal nerve courses in paraffin-embedded sagittal sections from 10 human fetuses, at 25-35 weeks of gestation, by using S100 protein immunohistochemical analysis. After diverging from the glossopharyngeal and vagus nerves at the level of the hyoid bone, the pharyngeal nerves entered the constrictor pharyngis medius muscle, then turned upward and ran superiorly and medially through the constrictor pharyngis superior muscle, to reach either the levator veli palatini muscle or the palatopharyngeus muscle. None of the nerves showed a tendency to run along the posterior surface of the pharyngeal muscles. Therefore, the pharyngeal nerve plexus in adults may become established by exposure of the fetal intramuscular nerves to the posterior aspect of the pharyngeal wall because of muscle degeneration and the subsequent rearrangement of the topographical relationship between the muscles that occurs after birth.
Investigation of Coelectroporation as a Method for Introducing Small Mutations into Embryonic Stem Cells (1992)

We have investigated coelectroporation as a method for introducing minor genetic changes into specific genes in embryonic stem cells. A selectable marker (neo) and a targeting replacement vector designed to insert a 4-bp insertion into exon 3 of the mouse hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene were coelectroporated into embryonic stem cells and selected in G418 and 6-thioguanine (6-TG). HPRT-negative clones were obtained at a frequency of approximately 1 per 520 G418r clones. Southern analysis and the polymerase chain reaction were used to demonstrate that 3 of 36 of the 6-TG-resistant clones had the desired 4-bp insertion without any other disruption of the HPRT locus. Initial studies indicated that the other 33 6-TG-resistant clones probably resulted from the targeted integration of a concatemer containing both the targeting construct and the selectable neo gene.
An Integrated Solid-State LED Luminaire for General Lighting (2009)

A strong systems approach to designing and building practical LED-based replacement lamps is lacking. The general method of taking high-performance LEDs and marrying them to standard printed circuit boards, drivers and a heat sink has fallen short of the promise of LED lighting. In this program, a top-down assessment of requirements and a bottom-up reinvention of LED sources, electronics, optics and mechanics have resulted in the highest performance lamp possible. The team, comprised of Color Kinetics, the leaders in LED lighting and Cree, the leaders in LED devices took an approach to reinvent the package, the driver and the overall form and aesthetic of a replacement source. The challenge was to create a new benchmark in LED lighting - the resultant lamp, a PAR38 equivalent, met the light output, color, color quality and efficacy marks set out in the program as well as being dimmable, which is important for market acceptance. The approach combined the use of multiple source die, a chip-on-board approach, a very efficient driver topology, the use of both direct emission and phosphor conversion, and a unique faceted optic to avoid the losses, artifacts and hotspots of lensed approaches. The integral heat sink provided a mechanical base and airflow using a chimney-effect for use in a wide variety of locations and orientations. These research results led to a much better understanding of the system effects of component level technologies. It was clear that best-of-breed sub-system results do not necessarily result in the best end result for the complete system. In doing this work, we did not neglect the practical aspects of these systems. These were not rarified results and commercially impractical but lent themselves to eventual commercial products in the marketplace. The end result - a high performance replacement lamp - will save significant energy while providing a high-quality light source.