gebhart/patrepeval huggingface dataset:
Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2 (2021)

The Dna2 helicase-nuclease functions in concert with the replication protein A (RPA) in DNA double-strand break repair. Using ensemble and single-molecule biochemistry, coupled with structure modeling, we demonstrate that the stimulation of S. cerevisiae Dna2 by RPA is not a simple consequence of Dna2 recruitment to single-stranded DNA. The large RPA subunit Rfa1 alone can promote the Dna2 nuclease activity, and we identified mutations in a helix embedded in the N-terminal domain of Rfa1 that specifically disrupt this capacity. The same RPA mutant is instead fully functional to recruit Dna2 and promote its helicase activity. Furthermore, we found residues located on the outside of the central DNA-binding OB-fold domain Rfa1-A, which are required to promote the Dna2 motor activity. Our experiments thus unexpectedly demonstrate that different domains of Rfa1 regulate Dna2 recruitment, and its nuclease and helicase activities. Consequently, the identified separation-of-function RPA variants are compromised to stimulate Dna2 in the processing of DNA breaks. The results explain phenotypes of replication-proficient but radiation-sensitive RPA mutants and illustrate the unprecedented functional interplay of RPA and Dna2.
Towards Atomic Level Understanding of Transition Alumina Phases and Their Phase Transformations (2015)

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Information Transmission and Temporal Code in Central Spiking Neurons (1997)

The brain encodes information in the intervals between the spikes which characterize neural firing events. Therefore it is relevant to study, in a timing code, how many spikes are necessary for reliably encoding input signals. We analyze the transmission of information, the reliability of signal detection, and the coding strategy for the case of central spiking neurons.
Controlled Microwave Irradiation for the Synthesis of YBa 2Cu 3O7-x Superconductors (1999)

For synthesizing a high-quality YBa 2 Cu 3 O 7- x superconductor using a domestic microwave oven without any post-heat treatment, the power and time of microwave irradiation are controlled by monitoring continuously the temperature of the microwave-heated sample. The temperature is measured by means of a metal-sheathed chromel-alumel thermocouple which is installed in the oven in the proper way. The conditions of the sample inside the oven are almost the same as the sample environment proposed by Kato et al. [Jpn. J. Appl. Phys. 36 (1997) L1291]. The maximum monitoring temperature where crystal growth occurs rapidly for a high-quality YBa 2 Cu 3 O 7- x superconductor is shown to be about 500°C which is much lower than the maximum heating temperature in the conventional method.
Selective and independent activation of four motor fascicles using a four contact nerve-cuff electrode (2004)

Any one of the four motor nerves in the cat sciatic nerve could be activated selectively and independently, from threshold to saturation, using a self-sizing spiral cuff electrode containing four radially placed monopolar contacts. These studies were carried out in nine adult cats with acute implants. Of the 36 possible fascicles, 23 fascicles could be activated selectively with current stimuli applied to a single contact and ten of the remaining fascicles could be activated selectively with current stimuli applied to two contacts, "field steering." In three experiments, time constraints precluded attempting selective activation through "field steering" techniques. In eight of the ten cases where "field steering" was used, a positive and a negative current source (anodic steering) were required to achieve the desired fascicle and in the remaining two cases, two negative current sources (cathodic steering) were required. The relative distance from the electrode contacts to each fascicle was well correlated to the order in which each fascicle was activated. In seven experiments, carried out in two animals, selective activation was verified by collision block techniques. The results of these experiments support the hypothesis that selective and independent activation of any of four motor fascicles in the cat sciatic nerve is possible using a four contact self-sizing spiral cuff electrode. Furthermore, in a more general case, these results support the concept of a "tunable" electrode that is capable of "steering" the excitation from an undesirable location to a preferred location.
Results of Implementation of the DigiScope for Diabetic Retinopathy Assessment in the Primary Care Environment (2006)

Approximately 50% of patients with diabetes in the United States do not undergo recommended ocular evaluations for diabetic retinopathy. The DigiScope (EyeTel Imaging, Inc., Columbia, MD) was developed as a cost-effective and practical telemedicine digital imaging system to screen for diabetic retinopathy in the primary care physician's (PCP) office. The DigiScope has been validated against seven-field stereo color fundus photography for the detection of diabetic retinopathy. This study reports on the implementation of the DigiScope for diabetic retinopathy assessment in the primary care environment. In PCP's offices, patients with diabetes who had not undergone an eye examination in the past year were imaged with the DigiScope. The images were transmitted to a reading center where the need for referral to an ophthalmologist was determined. Nonurgent referral was recommended for patients with diabetic retinopathy greater than a few micro-aneurysms, other ocular pathology, or unreadable images. Referral was deemed "urgent" for patients with sight-threatening disease and evaluation by an ophthalmologist within 72 hours was recommended. Between October 1, 2002 and March 31, 2771 patients with diabetes underwent DigiScope imaging at multiple sites. Nonurgent referral was recommended for 468 patients (17%). Urgent referral was recommended for 71 patients (3%). The images were unreadable in 295 cases (11%). This study indicates that implementation of the DigiScope in the primary care setting is practical and allows screening of patients with diabetes who are otherwise not receiving recommended eye examinations.
Genome Sequence of Megasphaera cerevisiae NSB1, a Bacterium Isolated from a Canning Line and Able To Grow in Beer with High Alcohol Content (2017)

The genome sequence of the brewery isolate <i>Megasphaera cerevisiae</i> NSB1 was determined. Strain NSB1 tolerates 5% (vol/vol) alcohol, which is higher than previously reported for <i>M. cerevisiae</i> The NSB1 genome will help elucidate genetics required for alcohol tolerance and niche adaptation of this Gram-negative beer-spoilage bacterium.
Practical durability requirements for self-compacting concrete (2008)

This paper is first giving an overview of hydration, pore structure, and transport mechanisms of self-compacting concrete (SCC). Afterwards available durability results are summarised keeping in mind the fundamental mechanisms and driving forces. Furthermore, the applicability to SCC of the durability requirements of the European Standard EN 206-1 is evaluated for the case of carbonation and chloride penetration. As some general and practical conclusion, it can be mentioned that the durability of self-compacting concrete is at least as good as the durability of traditional concrete with similar water/cement ratio and cement content. However, when the comparison is made based on the concrete strength, self-compacting concrete sometimes might show a somewhat inferior durability.
Decentralized Control Framework and Stability Analysis for Networked Control Systems (2014)

The combination of decentralized control and networked control where control loops are closed through a network is called decentralized networked control system (DNCS). This paper introduces a general framework that converts a generic decentralized control configuration of non-networked systems to the general setup of networked control systems (NCS). Two design methods from the literature of decentralized control for non-networked systems were chosen as a base for the design of a controller for the networked systems, the first being an observer-based decentralized control, while the second is the well-known Luenberger combined observer–controller. The main idea of our design is to formulate the DNCS in the general form and then map the resulting system to the general form of the NCS. First, a method for designing decentralized observer-based controller is discussed. Second, an implementation using a network is analyzed for the two designs. Third, two methods to analyze the stability of the DNCS are also introduced. Fourth, perturbation bounds for stability of the DNCS have been derived. Finally, examples and simulation results are shown and discussed.
Combined enhanced fluorescence and label-free biomolecular detection with a photonic crystal surface (2007)

A 2D photonic crystal surface with a different period in each lateral direction is demonstrated to detect biomolecules using two distinct sensing modalities. The sensing mechanisms both rely on the generation of a resonant reflection peak at one of two specific wavelengths, depending on the polarization of light that is incident on the photonic crystal. One polarization results in a resonant reflection peak in the visible spectrum to coincide with the excitation wavelength of a fluorophore, while the orthogonal polarization results in a resonant reflection peak at an infrared wavelength which is used for label-free detection of adsorbed biomolecules. The photonic crystal resonance for fluorescence excitation causes enhanced near fields at the structure surface, resulting in increased signal from fluorophores within 100 nm of the device surface. Label-free detection is performed by illuminating the photonic crystal with white light and monitoring shifts in the peak reflected wavelength of the infrared resonance with a high-resolution imaging detection instrument. Rigorous coupled-wave analysis was used to determine optimal dimensions for the photonic crystal structure, and devices were fabricated using a polymer-based nanoreplica molding approach. Fluorescence-based and label-free detection were demonstrated using arrays of spots of dye-conjugated streptavidin. Quantification of the fluorescent signal showed that the fluorescence output from protein spots on the photonic crystal was increased by up to a factor of 35, and deposited spots were also imaged in the label-free detection mode.
Figure S2 from A Bifunctional MAPK/PI3K Antagonist for Inhibition of Tumor Growth and Metastasis (2023)

&lt;p&gt;Supplemental Figure 2&lt;/p&gt;
The boundary layer on a flat plate in hypervelocity flow (1996)

Abstract The high enthalpy and high Mach number effects on flat plate boundary layer flow are examined. In particular, the behaviour of the density profile under high enthalpy, real gas conditions is considered. Mach-Zehnder interferograms of the flow over a flat plate are analysed using a two-dimensional Fourier transform procedure in order to obtain the density field. The comparison between theory and experiment is only fair. It must be pointed out, however, that the real gas effects on the density profiles were found to be small. Estimation of the velocity boundary layer thickness as obtained from a measurement of the thermal boundary layer thickness is seen to compare well with a theoretical prediction.
Mechanical Property Evolution during Autoclaving Process of Aerated Concrete Using Slag: I, Tobermorite Formation and Reaction Behavior of Slag (1994)

The chemical reactions in the formation of autoclaved aerated concrete (AAC) block were investigated. The samples were prepared using blast furnace slag at 180°C under saturated steam pressures for various times from 1 to 128 h. Autoclaving for 1 h yielded 1.1‐nm tobermorite with higher Al substitution and lower Ca/(Al + Si) ratio than that made without slag, due to the high solubility of the slag during the initial stage of reaction. This suggests that the utilization of slag has an advantage of reducing processing time. On further reaction, the crystallinity of the tobermorite increases and its Ca/(Al + Si) ratio decreases, becoming constant after 64 h. After a long period of autoclaving. thick reaction rims are formed around unreacted slag particles, interrupting the diffusion of Ca from slag to matrix.
Antineuronal Antibodies in Movement Disorders (1993)

The data strongly suggest an association between antecedent group A beta-hemolytic streptococcal infection as inferred from elevated antistreptococcal titers and the presence of serum antineuronal antibodies, which may, in turn, be linked to childhood movement disorders.
Mechanical Properties and Morphology of Ternary PP/EPDM/PE Blends (2008)

The ternary blends of high-density polyethylene (PE), EPDM terpolymer and polypropylene (PP) have been used as a model low interfacial tension system to study encapsulation dynamics in ternary blends and their relation to the blends' mechanical properties. It was found that the modulus, tensile strength and impact resistance can be improved by PE addition if the PE is localized within the EPDM phase. A range of blend morphology was found depending on the PE viscosity and polymer incorporation sequence in the twin-screw extruder. In the most favorable sequence, PE and EPDM were mixed together prior to their dispersion in the PP matrix. This practice resulted in a 50% increase in impact resistance when compared to mixing the three components in a single-step. Un mélange de polyéthylène de haute densité (PE), d'un terpolymère EPDM et de polypropylène (PP) a été utilisé à titre de système modèle pour étudier la dynamique d'encapsulation dans les mélanges ternaires et la relation entre les morphologies obtenues et la résistance mécanique. La rigidité, la résistance en traction et la résistance à l'impact furent améliorées par la présence du PE lorsque celui-ci se trouve incorporé à l'intérieur des particules d'EPDM. Plusieurs types de morphologies ont été obtenus selon la viscosité du PE utilisé et la séquence d'incorporation. Dans le cas le plus favorable, le PE et l'EPDM furent mélangés ensemble avant leur dispersion dans la matrice PP. Cette pratique a résulté en une augmentation de la résistance au choc de 50% comparativement au même mélange préparé en une seule et même étape.
Winding inductances of fractional slot surface-mounted permanent magnet brushless machines (2009)

Purpose Permanent magnet (PM) brushless machines equipped with fractional-slot concentrated-windings (FSCW) have been receiving considerable attention over the past few years, due to the fact that they have short end-windings, a high-slot fill factor, a high efficiency and power density, and good flux-weakening and fault-tolerance capabilities. A key design parameter for such machines is the phase winding inductance since this has a significant impact on the performance, as well as on the magnitude of any reluctance torque. The purpose of this paper is to describe a detailed investigation of the various components of the winding inductance in machines equipped with both overlapping and non-overlapping windings and different slot/pole number combinations. It also examines the influence of key design parameters, which affect the inductance components, with particular reference to the inductances of machines in which all the teeth are wound and those in which only alternate teeth are wound. Design/methodology/approach The paper analyzes and compares various inductance components which result from different winding configurations. Findings It is shown that the main component of the winding inductance is the relatively large slot-leakage component. Both analytical and finite element models are employed and predicted results are validated on several prototype machines. Originality/value Such a thorough investigation of the various inductance components for these type of machines has not been presented before. The paper will serve as a good reference for engineers and researchers designing PM machines equipped with FECW.
Tunable vertical-cavity surface-emitting laser with feedback to implement a pulsed neural model 1 Principles and experimental demonstration (2007)

An optoelectronic implementation of a modified FitzHugh-Nagumo neuron model is proposed, analyzed, and experimentally demonstrated. The setup uses linear optics and linear electronics for implementing an optical wavelength-domain nonlinearity. The system attains instability through a bifurcation mechanism present in a class of neuron models, a fact that is shown analytically. The implementation exhibits basic features of neural dynamics including threshold, production of short pulses (or spikes), and refractoriness.
GENETIC FINGERPRINTING OF THE IRISH APPLE COLLECTION USING SINGLE SEQUENCE REPEATS IN MULTIPLEX SYSTEMS - VALIDATION OF PROTOCOL (2011)

ISHS XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): III International Symposium on Plant Genetic Resources GENETIC FINGERPRINTING OF THE IRISH APPLE COLLECTION USING SINGLE SEQUENCE REPEATS IN MULTIPLEX SYSTEMS - VALIDATION OF PROTOCOL
Ion channel characterization using single cell impedance spectroscopy (2006)

A micro electrical impedance spectroscopy system (microEIS) for single cell analysis has been developed and used to differentiate ion channel activities of bovine chromaffin cells. K+ and Ca2+ channels were blocked and their electrical impedances were measured over a frequency range of 100 Hz to 5.0 MHz and compared to that of unblocked chromaffin cells. When ion channels were blocked, an increase in magnitude and decrease in phase of the measured impedances were observed. This result demonstrates that ion channel activities can be distinguished using the developed microsystem and it is expected that this system can be used to provide positive/negative information of ion channel blockage in a high throughput screening setup.
ChemInform Abstract: A Novel Epoxy‐Triterpene and Nortriterpene from Callus Cultures of Tripterygium wilfordii. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Carbon dioxide permeable tubings for post-suppression in ion chromatography (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon dioxide permeable tubings for post-suppression in ion chromatographyThomas. Sunden and Anders. CedergrenCite this: Anal. Chem. 1984, 56, 7, 1085–1089Publication Date (Print):June 1, 1984Publication History Published online1 May 2002Published inissue 1 June 1984https://pubs.acs.org/doi/10.1021/ac00271a009https://doi.org/10.1021/ac00271a009research-articleACS PublicationsRequest reuse permissionsArticle Views126Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Catalysts and Batteries Recycling to Produce Ferroalloys and High‐Speed Steel (2022)

To decrease its CO 2 emission and environmental footprint, Erasteel produces its high‐speed steel using 90% recycled materials and targets to increase this ratio even further. The remaining 10% are mainly metallic oxides (V 2 O 5 , MoO 3 ), ferroalloys (FeW, FeCr, FeSi), and cobalt. The total life assessment shows that producing from 100% recycled material leads to reducing CO 2 emission by 96%. The high‐value metallic alloys are sourced from spent catalysts, scraps, metallic oxides, or carbides. Used catalysts contain Mo, W, and Co elements that are directly used as raw material in high‐speed steel containing cobalt. The metals contained in the spent catalyst (Ni, Co, Mo) are recycled with specific equipment: a patented roaster to eliminate the sulfur and hydrocarbides, submerged electrodes electric furnace to separate metals and minerals and to obtain ferroalloys, an argon oxygen decarburization reactor to remove impurities and reduce oxides by aluminum or ferrosilicon additions. The elements contained in the batteries: Ni, Mn, and Zn, are separated by pyrometallurgy in an electric arc furnace to produce ZnO powder, MnO‐rich slag, and ferronickel alloys, the three of them used as raw material by metallurgical industries.
CSD2, CSD3, and CSD4, Genes Required for Chitin Synthesis in Saccharomyces cerevisiae: the CSD2 Gene Product Is Related to Chitin Synthases and to Developmentally Regulated Proteins in Rhizobium Species and Xenopus laevis (1992)

In Saccharomyces cerevisiae, chitin forms the primary division septum and the bud scar in the walls of vegetative cells. Three chitin synthetic activities have been detected. Two of them, chitin synthase I and chitin synthase II, are not required for synthesis of most of the chitin present in vivo. Using a novel screen, I have identified three mutations, designated csd2, csd3, and csd4, that reduce levels of chitin in vivo by as much as 10-fold without causing any obvious perturbation of cell division. The csd2 and csd4 mutants lack chitin synthase III activity in vitro, while csd3 mutants have wild-type levels of this enzyme. In certain genetic backgrounds, these mutations cause temperature-sensitive growth on rich medium; inclusion of salts or sorbitol bypasses this phenotype. Gene disruption experiments show that CSD2 is nonessential; a small amount of chitin, about 5% of the wild-type level, is detected in the disruptants. DNA sequencing indicates that the CSD2 protein has limited, but statistically significant, similarity to chitin synthase I and chitin synthase II. Other significant similarities are to two developmental proteins: the nodC protein from Rhizobium species and the DG42 protein of Xenopus laevis. The relationship between the nodC and CSD2 proteins suggests that nodC may encode an N-acetylglucosaminyltransferase that synthesizes the oligosaccharide backbone of the nodulation factor NodRm-1.