gebhart/patrepeval huggingface dataset:
A versatile microfluidic platform for the study of cellular interactions between endothelial cells and neutrophils General subjects (2017)

Endothelial migration is a critical physiological process during vascular angiogenesis, growth and development, as well as in various disease conditions, such as cancer and cardiovascular diseases. Neutrophil migration, known as the important characteristic of immune responses, is also recognized as a contributor to the diseases involving endothelial migration. Herein, the mutually dependent relationship between neutrophil recruitment and endothelial migration was studied on a microfluidic platform for the first time.An in vivo-like microenvironment is created inside microfluidic devices by embedding a gel scaffold into the micro-chambers. This approach, with controllable stable chemical gradients and the ability to quantitate interaction characteristics, overcomes the limitations of the current in vivo and in vitro assays for cell migration studies.The number of neutrophils migrating through the endothelial cell layer is heavily influenced by the concentration of vascular endothelial growth factor (VEGF) that induces endothelial cell migration in the gel scaffold, and is not as correlated to the concentration of chemokine solution used for initiating neutrophil migration. More importantly, neutrophil migration diminishes the effects of the drug that inhibits endothelial migration and this process is regulated by the concentration of chemokine molecules instead of VEGF concentration.The results presented herein demonstrate the complicated cellular interactions between endothelial cells and neutrophils: endothelial migration delicately regulates neutrophil migration while the presence of neutrophils stabilizes the structures of endothelial migration.This study provides deeper understanding of the dynamic cellular interactions between neutrophils and endothelial cells as well as the pathogenesis of relevant diseases.
Neuropsychological test performance of children with ADHD relative to test norms and parent behavioral ratings (2002)

Neuropsychological test performance relative to published norms and parent ratings was assessed archivally among 78 children with attention deficit hyperactivity disorder (ADHD). Despite average to low average IQs and academic achievement, the children performed poorly relative to test norms on most measures sensitive to fronto-executive functioning (span of attention, sustained attention, response inhibition, and working memory). They also performed poorly on most memory tests requiring free recall/retrieval, a skill dependent in part on intact frontal/subcortical functioning. In contrast, performance fell within the average to low average range on all measures of retention, although some mean scores fell significantly below normative means. In addition, higher levels of inattention or hyperactivity as assessed from parent reports were associated with worse performance on neuropsychological tests. That is, correlations calculated between neuropsychological test scores and parent ratings on the Adaptive Behavior Scale (ABS) were both significant at the P<.05 level and in the expected direction in 33% of the analyses for ratings of attention, in 43% of the analyses for ratings of hyperactivity, and in 5% of the analyses for ratings of persistence.
Met oncogene aberrant expression in canine osteosarcoma (2000)

The objective of this study was to investigate the role of the MET oncogene in canine osteosarcoma. Seven large-breed dogs affected by spontaneous skeletal osteosarcoma underwent en bloc tumor excision. Total RNA was extracted from frozen tumor samples and assessed for expression of the MET oncogene by Northern blot analysis. Five of seven biopsy samples expressed high levels of the MET oncogene; its expression in the primary tumors was comparable with that previously identified in primary osteosarcomas in humans. A lung metastasis from one of the dogs expressed MET at a higher level than did its primary tumor. Spontaneously arising osteosarcoma in dogs clinically and pathologically mimics the corresponding disease in humans. We previously demonstrated that the MET oncogene was aberrantly expressed in a high percentage of human osteosarcomas. The results of the current study also provide a molecular parallel between the tumors in dogs and humans. This in vivo model may be helpful in evaluating new strategies for therapy against osteosarcoma.
Nuclear Dot Antigens may Specify Transcriptional Domains in the Nucleus (1993)

A bank of 892 human autoimmune serum samples was screened by indirect immunofluorescence on human tissue culture HT-29 cells. Seven serum samples that stain 4 to 10 bright dots in cell lines of several different mammals, including humans, monkeys, rats, and pigs, were identified. Immunofluorescence experiments indicate that these antigens, called nuclear dot (ND) antigens, are distinct from splicing complexes, kinetochores, and other known nuclear structures. An ND antigen recognized by these sera was cloned by immunoscreening a human lambda gt11 expression library. Analysis of seven cDNA clones for the ND antigen indicates that several mRNAs exist, perhaps derived through alternative splicing mechanisms. One major form of the message has an open reading frame of 1,440 bp capable of encoding a 53,000-M(r) protein. Treatment of cells with detergent, salt, or RNase A fails to remove the ND antigen from the nucleus. However, incubation with DNase I obliterates ND staining, indicating that the ND protein directly or indirectly associates with nuclear DNA. Fusion of the ND protein to a LexA DNA binding domain activates transcription in Saccharomyces cerevisiae. A 75-amino-acid domain that activates transcription in both yeast and primate cells has been identified. We suggest that ND antigens may participate in the activation of transcription of specific regions of the genome.
A simplified procedure for semi-targeted lipidomic analysis of oxidized phosphatidylcholines induced by UVA irradiation (2012)

Oxidized phospholipids (OxPLs) are increasingly recognized as signaling mediators that are not only markers of oxidative stress but are also "makers" of pathology relevant to disease pathogenesis. Understanding the biological role of individual molecular species of OxPLs requires the knowledge of their concentration kinetics in cells and tissues. In this work, we describe a straightforward "fingerprinting" procedure for analysis of a broad spectrum of molecular species generated by oxidation of the four most abundant species of polyunsaturated phosphatidylcholines (OxPCs). The approach is based on liquid-liquid extraction followed by reversed-phase HPLC coupled to electrospray ionization MS/MS. More than 500 peaks corresponding in retention properties to polar and oxidized PCs were detected within 8 min at 99 m/z precursor values using a single diagnostic product ion in extracts from human dermal fibroblasts. Two hundred seventeen of these peaks were fluence-dependently and statistically significantly increased upon exposure of cells to UVA irradiation, suggesting that these are genuine oxidized or oxidatively fragmented species. This method of semitargeted lipidomic analysis may serve as a simple first step for characterization of specific "signatures" of OxPCs produced by different types of oxidative stress in order to select the most informative peaks for identification of their molecular structure and biological role.
Reducing operating time of a crawling robot for epicardial surgery (2010)

HeartLander is a small, mobile robot designed to assist with surgical procedures on the surface of the heart. It crawls within the pericardial sac surrounding the heart. The friction forces HeartLander experiences from the pericardium and the heartbeat reduce locomotion efficiency. We have developed an algorithm that creates a plan for reaching a set of treatment targets, while minimizing the time spent in locomotion. Results from simulation show improvement over a simple greedy technique.
The GPRS Mobile Payment System Based on RFID (2006)

As a kind of mobile data service, mobile payment service has approached people's lives. It becomes the focus and will be a field where telecom service providers compete. After analyzing the current mobile payment service in China, this paper puts forward an optimization design of the GPRS mobile payment system based on radio frequency identification (RFID) as a transitional solution before the prevalence of the NFC (near field communication) cellphone. And then the system structure, service flow and security are described. By virtue of RFID, the telecom service provider can provide diversified mobile payment services with high privacy. On the other hand, the framework of the system is simple and takes full advantage of the existing GSM mobile communication network compared with the mobile payment system based on CDMA in Japan and Korea. Moreover, this system does not require user to change their cellphone to support RFID chip.
On the efficient allocation of resources for hypothesis evaluation: a statistical approach (1995)

This paper considers the decision-making problem of selecting a strategy from a set of alternatives on the basis of incomplete information (e.g. a finite number of observations). At any time the system can adopt a particular strategy or decide to gather additional information at some cost. Balancing the expected utility of the new information against the cost of acquiring the information is the central problem that the authors address. In the authors' approach, the cost and utility of applying a particular strategy to a given problem are represented as random variables from a parametric distribution. By observing the performance of each strategy on a randomly selected sample of problems, one can use parameter estimation techniques to infer statistical models of performance on the general population of problems. These models can then be used to estimate: (1) the utility and cost of acquiring additional information and (2) the desirability of selecting a particular strategy from a set of choices. Empirical results are presented that demonstrate the effectiveness of the hypothesis evaluation techniques for tuning system parameters in a NASA antenna scheduling application.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Mastering Web Services Security (2005)

We present material on how to use the architectures and technologies and how to understand the specifications that are available to build a secure Web Services system. Since this technology is rapidly changing, we present the theory behind the models and explain the thinking behind many of the security specifications that are at the forefront of the technology today. Our emphasis is on showing you how to build and understand the complexities of a secure end-to-end Web Services system. This book gives you both a detailed technical understanding of the major components of an end-to-end enterprise security architecture and a broad description of how to deploy and use Web Services security technologies to protect your corporation and its interaction with the outside world.
Orthotopic liver transplantation: An experimental study on the prevention of infections with Gram-negative organisms (1975)

Infection with Gram-negative bacteria is frequently the cause of death in human liver transplantation. In experimental liver transplantation in mongrel dogs we detected Gram-negative septicaemia in 73% of our first 15 cases; maximal survival was 26 days. In a second series of 27 orthotopic liver transplantations in tissue-typed littermate dogs and littermate pigs the standard surgical technique and aftercare were changed with regard to four factors: an end-to-end common bile duct anastomosis was made instead of a gallbladder to duodenum anastomosis; the continuous postoperative bacteriostatic antibiotic therapy was changed to a single 2-day course of bactericidal antibiotics; preoperative selective bowel decontamination combined with postoperative protective isolation was introduced; the dosage of azathioprine used for immunosuppression was reduced..
Use of Halophilic Bacteria in Biological Treatment of Saline Wastewater by Fed‐Batch Operation (2000)

Biological treatment of saline wastewater by standard activated‐sludge cultures typically results in low chemical oxygen demand (COD) removal efficiencies as a result of plasmolysis of cells caused by high salt content (&gt; 1%). Removal of salt from wastewater before biological treatment by reverse osmosis or ion exchange would be quite costly. However, inclusion of salt‐tolerant organisms in an activated‐sludge culture to improve treatment efficiency is a practical approach developed and presented in this article. Synthetic wastewater composed of diluted molasses, urea, phosphate, and different amounts of salt (0 to 5% sodium chloride) was treated in an aeration tank operated in fed‐batch mode. Halobacter halobium added to activated‐sludge culture was used in biological treatment, and results were compared with those obtained with the activated‐sludge culture alone. Halobacter addition produced significantly greater COD removal rates and efficiencies (% removal) at salt concentrations greater than 2% salt. At low salt concentrations, performances of both cultures were comparable. Results indicated that saline wastewater containing more than 2% salt can be treated effectively by Halobacter ‐supplemented, activated‐sludge cultures, with COD removal efficiencies greater than 85% within 9 hours of fed‐batch operation.
Seismic Driven 3D Geomechanical Model of Lower Paleozoic Shale Formation (Eastern Europe) – Case Study (2016)

Summary The subject of this study is the characterization of geomechanical parameters of Lower Paleozoic shale formation (Eastern Europe). In the paper results of 3D geomechanical model together with applied methods and comprehensive workflow are presented. Characterization of petrophysical and mechanical properties, pore pressure, in situ stresses of shale formation was carried based on seismic, well log and laboratory data. Resulting from the study geomechanical model can be used afterwards in aware, multistage – decision making during reservoir lifetime.
Feasibility and Initial Results of an Internet‐Based Pacemaker and ICD Pulse Generator and Lead Registry (2001)

The medical community has no independent source of timely information regarding the performance of pacemaker and ICD pulse generators and leads. Accordingly, the authors established an Internet-based registry of pacemaker and ICD pulse generator and lead failures (www.pacerandicregistry.com). During the first year, they found three previously unreported device problems that were promptly communicated to the participants. Of the failures reported, 11% of ICD and 10% of pacemaker pulse generator failures were heralded by signs other than the expected elective replacement indicator (ERI). Average ICD battery longevity was 4.0 +/- 0.7 years, and average dual chamber pacemaker battery longevity was 6.8 +/- 2.6 years. Disrupted insulation accounted for 54% of pacemaker and 29% of ICD lead failures. Compared to pacemaker pulse generator and lead failure, ICD device failures were more likely to cause severe clinical consequences. In conclusion, an Internet-based registry is feasible and capable of providing timely data regarding the signs, causes, and clinical consequences of pacemaker and ICD failures.
Modern web attacks (2008)

There are many factors that can dictate the success of a piece of malware. These include how and to whom it is delivered, how it is executed, how rapidly it propagates and how successfully it evades detection. The first two of these describe the process of threat delivery and execution, which are perhaps the most influential factors in the success of a threat. Traditionally cybercriminals have used email as their preferred vector of attack, employing various social engineering tactics in order to entice the recipient into executing the malicious attachment. As companies have become more aggressive in blocking email content, criminals have shifted their attentions, and are now firmly focused on the web.
Large-signal Affine representation for nonlinear microwave circuits direct synthesis (1995)

An exact direct synthesis method for nonlinear microwave circuits is proposed. It makes use of a large-signal affine representation of the nonlinear circuit directly derived from a standard Harmonic Balace analysis. This representation allows direct linear synthesis methods and is therefore an alternative to non-linear optimisation methods.
11C-labelling of substance P. Preparation of a homocysteine-containing precursor and its subsequent application in the synthesis of the labelled neuropeptide (1987)

The synthesis of a useful [Hcy11]Substance P derivative (16), containing only two protecting groups both labile to sodium in liquid ammonia, by a multistep [3 +(4 + 4)] strategy in solution, is reported. This approach furnished solid intermediates in high yields. After removal of the protecting groups, the product was selectively methylated on sulphur in situ with the aid of [11C]methyl iodide as previously described for Met-enkephalin to give [S-methyl-11C] Substance P (18) in a radiochemical yield of 35%. The total time of preparation was 45–60 min from the start of the synthesis of [11C]methyl iodide. After purification by h.p.l.c., the peptide exhibited a radiochemical purity higher than 98%. An intermediary Substance P4–11 fragment was also labelled.
Transmission of 32 channels at 10 Gbit/s over 450km of dispersion managed cable using LEAF™ and sub-LS™ fibres (1999)

The transmission of 32 dense wavelength division multiplexing (DWDM) channels at 10 Gbit/s over 450 km of dispersion managed cable, using LEAF™ and sub-LS™ fibres, is demonstrated. The use of LEAF™ fibre in the high power region of transmission effectively reduces any nonlinear effects. Q values ranging from 7.5 to 11.1 (bit error rate (BER) between 2 × 10–14 and 6.3 × 10–29) were obtained for all channels without any extra dispersion compensation at the transmitter or receiver.
Effect of a Skating Unit on Fitness in Fifth-Grade Students (2021)

This study investigated the effect of a skating unit on cardiovascular fitness, eyes-closed static balance, explosive power, and agility in fifth graders. During a 6-week skating unit (12 lessons), 71students (Mage = 10.34 years, range: 10–12 years) participated during regularly scheduled physical education classes. Three classes were involved, one serving as the control group and the other two as experimental groups (roller skating and in-line skating). The control group had no access to roller or in-line skates during class time, rather the curriculum consisted of soccer, dance, and softball. The experimental groups participated in a specifically designed skating curriculum adapted with permission from Skatetime and Skate in School. Results showed that the roller skating group had longer static balance times than the in-line skating group. Even though other factors reported nonsignificance, difference scores illustrated gains after intervention, suggesting that a skating unit could induce improvements. Further research examining significance between roller skating and in-line skating is warranted.
Geodesic Object Representation and Recognition (2003)

This paper describes a shape signature that captures the intrinsic geometric structure of 3D objects. The primary motivation of the proposed approach is to encode a 3D shape into a one-dimensional geodesic distribution function. This compact and computationally simple representation is based on a global geodesic distance defined on the object surface, and takes the form of a kernel density estimate. To gain further insight into the geodesic shape distribution and its practicality in 3D computer imagery, some numerical experiments are provided to demonstrate the potential and the much improved performance of the proposed methodology in 3D object matching. This is carried out using an information-theoretic measure of dissimilarity between probabilistic shape distributions.
Use of Ultrasound Backfat Estimates to Form Marketing Groups Prior to Finishing for Feedlot Steers (1998)

An experiment was conducted using 95 Continental crossbred steers. The cattle were sorted by ultrasound 160 days before slaughter into a low backfat group (Low BF) and a higher backfat group (High BF). Half of the Low BF and half of the High BF were implanted whereas the other halves were not. Data from the experiment were used in two hypothetical markets. One market was a high yield beef program (HY) that did not allow the use of implants. The second market was a commodity beef program (CM) that allowed the use of implants. The cattle were priced as an unsorted group (ALL) and two sorted groups (Low BF and High BF) within the HY (non-implanted) and CM (implanted) markets. The CM program had a base price of $1.05/lb hot carcass weight (HCW) with a $0.15/lb HCW discount for quality grade (QG) Select and a $0.20/lb HCW discount for yield grade (YG) 4. The HY program used a base price of $1.07/lb HCW with premiums ($/lb HCW) paid for YG £ .9 (.15), 1.0 - 1.4 (.10), and 1.5 - 1.9 (.03). The carcasses were discounted ($/lb HCW) for YG 2.5 - 2.9 (.03), 3.0 - 3.9 (.15), and ³ 4.0 (.35). This data set provides good evidence that the end point at which to sell a group of cattle depends on the particular market. Sorting had an economic advantage over ALL in the HY Low BF and the CM High BF groups. The HY High BF cattle should have been sold sooner due to the discounts recieved for increased YG. The increased YG was directly affected by an increase in BF. Furthermore, the CM Low BF group should have been fed longer to increase the number of carcasses grading Choice.
Global structural changes (1987)

The biological importance of large scale intramolecular motions is obvious. Such motions are an essential part of protein folding, the binding of ligands by proteins, and allosteric effects in enzymes. They are important in a variety of interactions between macromolecules, including the aggregation of antibodies (Yguerabide, Epstein & Stryer, 1970; Hanson, Yguerabide & Schumaker, 1985), the formation of the protein coat of viruses (Harrison, 1978), muscle contraction (Huxley, 1969; Harrington, 1971; Harvey & Cheung, 1982; Eisenberg & Hill, 1985), and the packaging of DNA in the nucleosome (Olins & Olins, 1974; Kornberg, 1974; Levitt, 1978; Sussman & Trifonov, 1978). They may be coupled to local intramolecular motions of the kind described in chapter 6, for example in the repuckering of sugars accompanying the transitions between the A, B and Z conformations in DNA, or in the formation of the environments necessary for some local structural transitions.
Na-hyaluronic acid 국소점안이 각막상피 찰과상 치유에 미치는 영향 (1996)

국소점안한 1% Na-hyaluronic acid(Na-HA)가 각막상피 치유에 미치는 영향을 알아보고자 각막 상피가 결손된 각막상피 찰과상 환자 24안을 대상으로, 치료군에는 1% Na-HA(Healon)를, 대조군에는 항생제 안연고(Tobrex ointment)를 각각 1일 4회 점안하고, 각막상피 결손부위를 6∼12시간 간격으로 완전치유가 될 때까지 photo-slit lamp로 촬영하여 두군간의 상피치유속도를 비교하였다. 상피치유속도는 치료군이 1.02±0.26mm2/hour, 대조군이 0.67±0.19mm2/hour로 치료군에서 유의하게 신속한 치유를 보였다(p<0.05). 본 연구의 결과는 국소점안한 Na-HA가 대조군에 비하여 각막상피치유를 촉진하는 효과가 있음을 시사하므로, 임상적으로 신속한 각막상피 재생이 요구되는 경우 Na-HA의 사용을 고려해 볼 수 있다(한안지 37:921∼926, 1996).
Synergistic design of a combined floating wind turbine - wave energy converter (2017)

Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2017.