MP20-07 ERYTHROPOIETIN PROMOTES THE REGENERATION OF URETERAL PERISTALSIS IN A MURINE MODEL OF TRANSIENT OBSTRUCTION. (2014)

You have accessJournal of UrologyStone Disease: Basic Research I1 Apr 2014MP20-07 ERYTHROPOIETIN PROMOTES THE REGENERATION OF URETERAL PERISTALSIS IN A MURINE MODEL OF TRANSIENT OBSTRUCTION. Claudia Janssen, Wolfgang Jäger, Igor Moskalev, Ladan Fazli, Joachim Thüroff, Ben Chew, and Dirk Lang Claudia JanssenClaudia Janssen More articles by this author , Wolfgang JägerWolfgang Jäger More articles by this author , Igor MoskalevIgor Moskalev More articles by this author , Ladan FazliLadan Fazli More articles by this author , Joachim ThüroffJoachim Thüroff More articles by this author , Ben ChewBen Chew More articles by this author , and Dirk LangDirk Lang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.729AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Obstructive uropathy can precipitate ureteral aperistalsis and ascending pyelonephritis. While peristaltic activity is lost in the dilated ureter during unilateral ureteral obstruction (UUO), the functional recovery of the ureter following obstruction removal remains unclear. Erythropoietin (EPO) has been shown to provide protective effects in non-hematopoietic organs and to restore peristalsis in hypo-contractile intestinal smooth muscle (SM). Here, we questioned the role of EPO in SM of the ureter and hypothesized that it may play a therapeutic role in restoring ureteral function following obstructive uropathy. METHODS RT-PCR was used to determine mRNA expression of EPO, Erythropoietin receptor (EpoR) and β-common receptor (βCR) in obstructed and un-obstructed ureters from mice. Mice underwent UUO using a non-traumatic microclip for 24h, 48h or 72h (n=22 animals/group). Ten mice per group received 200IU of EPO on 4 consecutive days; controls received saline. The duration from removal of the obstruction until regression of hydronephrosis was assessed daily with ultrasonography. Peristalsis was assessed microscopically prior- and post-removal of the obstruction via laparatomy. RESULTS EPO, EPOR and βCR were expressed in murine ureters and endogenous EPO expression was up-regulated in obstructed ureters. Following obstruction removal, progressive hydronephrosis and impaired peristaltic activity correlated with the duration of obstruction. Resolution of hydronephrosis and restoration of ureteral peristalsis was significantly accelerated in EPO treated animals compared to controls. CONCLUSIONS This study indicates that Erythropoietin signalling is present in the murine ureter and demonstrates that the external application of EPO enhances the functional recovery of the ureter resulting in protection of the upper urinary tract. Thus, administration of EPO may present a novel treatment strategy to maintain ureteral SM function in the face of obstructive uropathy. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e198 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Claudia Janssen More articles by this author Wolfgang Jäger More articles by this author Igor Moskalev More articles by this author Ladan Fazli More articles by this author Joachim Thüroff More articles by this author Ben Chew More articles by this author Dirk Lang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...