gebhart/patrepeval huggingface dataset:
The Therapeutic Effects of Adipose-Derived Stem Cells and Recombinant Peptide Pieces on Mouse Model of DSS Colitis (2018)

Cell therapies using adipose-derived stem cells (ADSCs) have been used to treat inflammatory bowel disease (IBD) in human and dog. We previously reported the CellSaic technique, which uses a recombinant scaffold to enhance the efficacy of cell therapy. To examine whether this technique can be applied to cell therapy for colitis, we evaluated the efficacy of CellSaic in colitis mouse models. Colitis mouse models were developed by administering dextran sulfate sodium (DSS) to C57BL/6 mice for 7 days. Then CellSaic comprising human/canine ADSCs (1.2 × 10<sup>6</sup> cells) or human/canine ADSCs only (1.2 × 10<sup>6</sup> cells) were administered to the mice. The body weights were measured, and the colon length measurements and histological evaluations were conducted at 7 days after administration. After in vitro culture of human ADSC (hADSC) CellSaic and hADSC spheroids in medium containing TNFα, the levels of the anti-inflammatory protein TSG-6 in each supernatant were measured. Furthermore, we conducted tumorigenicity and general toxicity tests of canine ADSC (cADSC) CellSaic in NOG mice for 8 weeks. In the colitis mouse models, the ADSC CellSaic group presented recovery of body weight and colon length compared with the ADSC-only group. Histological analysis showed that ADSC CellSaic decreased the number of inflammatory cells and repaired ulceration. In vitro, hADSC CellSaic secreted 3.1-fold more TSG-6 than the hADSCs. In addition, tumorigenicity and general toxicity of cADSC CellSaic were not observed. This study suggests that human and canine ADSC CellSaic has a therapeutic effect of colitis in human and dogs.
The subcellular localization and length of hammerhead ribozymes determine efficacy in human cells (1997)

The length requirements of the antisense portion of hammerhead ribozymes for efficacy in living cells was investigated. The HIV-1tat-directed asymmetric hammerhead ribozyme alphaYRz195 was used with a 195 nt 3'-antisense arm and a 3 nt 5'-antisense portion as well as a set of successively 3'-shortened derivatives thereof. In the 3'-antisense arm a minimum length of 20 complementary nucleotides was required for efficient association with a 645 nt target RNA transcript in vitro(for all constructs kass ranged between 0.3 and 1.8x104/M/s). The cleavage rate constants (kcleav) were independent of the length of the antisense flank and ranged between 0.8 and 1.2x10-4/s. However, the length of the antisense arms, as well as the mode of delivery and the subcellular location of the ribozymes, had a dramatic effect on efficacy in HIV-1-producing human cells. When proviral HIV-1 DNA and ribozymes were co-microinjected into the nucleus of human cells, a minimum length of 51 nt in the antisense arm was necessary for antisense- and ribozyme-mediated inhibition of HIV-1 replication. Ribozymes with shorter antisense arms were almost ineffective. Conversely, short chain ribozymes, including those with chemical modifications, were superior to long chain ribozymes when co-microinjected into the cytoplasm. When transfected, all ribozymes showed an antisense effect as well as an additional ribozyme-mediated increase in inhibition. Consequences for the design and application of ribozymes are discussed.
Hydroxylation of alkanes by molecular oxygen with dinuclear FeII macrocyclic complexes as catalysts (1998)

The iron(II) complexes of two new macrocyclic ligands: [24]RBPyBC (L24), a 24-membered macrocycle containing phenol, pyridine and amino donor groups, and [30]RBBPyBC (L30), a 30-membered macrocycle containing phenol, bipyridine, and amino donor groups, were found to be effective for the hydroxylation of alkanes, including cyclohexane and adamantane, with H2S as a two-electron reductant.
The structure and fragmentation of Bn (n≥3) ions in peptide spectra (1996)

The unimolecular and low energy collision-induced fragmentation reactions of the MH(+) ions of N-acetyl-tri-alanine, N-acetyl-tri-alanine methyl ester, N-acetyl-tetra-alanine, tetra-alanine, penta-alanine, hexa-glycine, and Leu-enkephalin have been studied with a particular emphasis on the formation and fragmentation of B n (n=3,4,5) ions. In addition, the metastable ion fragmentation reactions of protonated tetra-glycine, penta-glycine, and Leu-enkephalin amide have been studied. B n ions are prominent stable species in all spectra. The B n ions fragment, in part, by elimination of CO to form A n ions; this reaction occurs on the metastable ion time scale with a substantial release of kinetic energy (T 1/2=0. 3-0. 5 eV) that indicates that a stable configuration of the B n ion fragments by way of a reacting configuration that is higher in energy than the fragmentation products, A n + CO. Ab initio calculations strongly suggest that the stable configuration of the B3 and B4 ions is a protonated oxazolone formed by interaction of the developing charge with the next-nearest carbonyl group as HX is lost from the protonated species H-(Yyy) n -X · H(+). The higher B n ions also fragment, in part, to form the next-lower B ion, presumably in its stable protonated oxazolone form. This reaction is rationalized in terms of the three-dimensional structure of the B n ions and it is proposed that the neutral eliminated is an α-lactam.
Microarray Meta-Analysis Identifies Acute Lung Injury Biomarkers in Donor Lungs That Predict Development of Primary Graft Failure in Recipients (2012)

Taken together, our data suggests that microarray analysis of gene expression data allows for the detection of "injury" gene predictors that can classify lung injury samples and identify patients at risk for clinically relevant lung injury complications.
Ring opening reactions of tetrathiacyclopropenonophanes and the subsequent formation of a molecular box stabilised by hydrogen bonds (2005)

Sulfur atoms next to a cyclopropenone moiety change their reactivity completely. Tetrathiacyclopropenonophanes 6 and 10 were treated with protic solvents such as methanol and water yielding ring opened diesters 9a and 9b, and the diacid 11, respectively. This observation is in contrast to alkyl and aryl substituted cyclopropenones which are stable in the presence of protic solvents. The molecular structures of the diester and the diacid could be elucidated by means of X-ray diffraction analyses. In the latter case a molecular box was formed in such a way that the two acid moieties dimerise via hydrogen bonding. The resulting hollow core is able to host one molecule of chloroform.
ChemInform Abstract: SRN1‐Reactivity of 1‐(α‐Nitroalkyl)imidazoles. Synthesis of Imidazoles Alkylated in Position 1 with Branched and Functionalized Side Chains. (1988)

Abstract The 1‐nitroalkylimidazoles (I) react with the nitronates (II) in the presence of NaH under photostimulation to give the substitution products (III) which are reduced to form the 1‐alkylated imidazoles (IV).
Performance evaluation for band-limited DS-CDMA communication system (2002)

The model uses a coherent binary phase shift keying (BPSK) modulation scheme operated in the asynchronous mode. For ease of interpretation of results, raised cosine spectra for filters is assumed. A standard Gaussian approximation is used to derive approximated error probabilities, P/sub e/, in simple form. The effect of imperfect despreading synchronization is shown.
Crystallization kinetics of dilute polyethylene solutions (1970)

Abstract The crystallization kinetics of a high molecular weight fraction of linear polyethylene was studied in dilute solutions of p ‐xylene, n ‐hexadecane, and decalin by dilatometric methods. For all solvents and temperatures, the experimental isotherms could be quantitatively described by the Avrami formulation for the complete transformation. This result is unique in the realm of polymer crystallization, since marked deviations from this theory are usually observed in more concentrated systems. The Avrami exponent is found to be n = 4 in all cases. The temperature coefficients of the rate constants are indicative of a nucleation controlled process. The data fit either a two‐dimensional or three‐dimensional nucleation mode, and a discrimination can not be made between these two cases. The interfacial free energies are found to be independent of the solvent medium. It is also shown that, irrespective of the type of nucleation control governing the kinetics, the same type governs the crystallite thickness of the lamella‐like crystals that are formed.
Development of a computerized method for identifying the posteroanterior and lateral views of chest radiographs by use of a template matching technique (2002)

In picture archiving and communications systems (PACS) or digital archiving systems, the information on the posteroanterior (PA) and lateral views for chest radiographs is often not recorded or is recorded incorrectly. However, it is necessary to identify the PA or lateral view correctly and automatically for quantitative analysis of chest images for computer-aided diagnosis. Our purpose in this study was to develop a computerized method for correctly identifying either PA or lateral views of chest radiographs. Our approach is to examine the similarity of a chest image with templates that represent the average chest images of the PA or lateral view for various types of patients. By use of a template matching technique with nine template images for patients of different size in two steps, correlation values were obtained for determining whether a chest image is either a PA or a lateral view. The templates for PA and lateral views were prepared from 447 PA and 200 lateral chest images. For a validation test, this scheme was applied to 1,000 test images consisting of 500 PA and 500 lateral chest radiographs, which are different from training cases. In the first step, 924 (92.4%) of the cases were correctly identified by comparison of the correlation values obtained with the three templates for medium-size patients. In the second step, the correlation values with the six templates for small and large patients were compared, and all of the remaining unidentifiable cases were identified correctly.
The USDA cucumber (Cucumis sativus L.) collection: genetic diversity, population structure, genome-wide association studies, and core collection development (2018)

Germplasm collections are a crucial resource to conserve natural genetic diversity and provide a source of novel traits essential for sustained crop improvement. Optimal collection, preservation and utilization of these materials depends upon knowledge of the genetic variation present within the collection. Here we use the high-throughput genotyping-by-sequencing (GBS) technology to characterize the United States National Plant Germplasm System (NPGS) collection of cucumber (Cucumis sativus L.). The GBS data, derived from 1234 cucumber accessions, provided more than 23 K high-quality single-nucleotide polymorphisms (SNPs) that are well distributed at high density in the genome (~1 SNP/10.6 kb). The SNP markers were used to characterize genetic diversity, population structure, phylogenetic relationships, linkage disequilibrium, and population differentiation of the NPGS cucumber collection. These results, providing detailed genetic analysis of the U.S. cucumber collection, complement NPGS descriptive information regarding geographic origin and phenotypic characterization. We also identified genome regions significantly associated with 13 horticulturally important traits through genome-wide association studies (GWAS). Finally, we developed a molecularly informed, publicly accessible core collection of 395 accessions that represents at least 96% of the genetic variation present in the NPGS. Collectively, the information obtained from the GBS data enabled deep insight into the diversity present and genetic relationships among accessions within the collection, and will provide a valuable resource for genetic analyses, gene discovery, crop improvement, and germplasm preservation.
Paramagnetic and diamagnetic gas separation by novel high-TC superconducting membrane (2004)

International audience
Age‐induced changes in cellular membranes of imbibed soybean seed axes (1988)

The physical and chemical properties of microsomal membranes and cellular antioxidant systems were investigated in imbibed soybean ( Glycine max L. Merr. cv. Maple Arrow) seeds following aging for 5 years at room temperature. The loss of germination capacity in aged seeds was associated with increased solute leakage during imbibition and with a loss of membrane phospholipid. Higher levels of free fatty acids were observed in the microsomal membranes from aged seeds. However, there was no change in fatty acid saturation. Wide angle X‐ray diffraction studies indicated the presence of gel phase in addition to liquid‐crystalline phase lipid domains in the membranes of aged seeds. Those from fresh seeds were exclusively liquid‐crystalline. Fluorescence depolarization, using diphenylhexatriene, suggested that the microviscosity of the membrane bilayer was increased by aging. Aged seeds had a lower antioxidant potential in the lipid fraction, lower tocopherol content, and reduced ascorbate:dehydroxyascorbate ratio indicating that the aging process was associated with exposure to an oxidative stress.
Recent Microseismic Monitoring Results from VSP and Permanent Sensor Deployments in Saudi Arabia (2009)

A large surface and downhole microseismic network is complemented with a temporary VSP tool deployment to investigate water flooding in Ghawar. Water injector rates are varied to induced microseismicity in the Arab-D reservoir to map possible fracture zones and flood front variations.
[Radial hemolysis in the diagnosis of influenza virus infections]. (1978)

Some of the parameters of the radial haemolysis test were studied, and the test was used to determine antibody titres of sera obtained from patients involved in the epidemic outbreak of influenza-A/Port Chalmers, 1975. The sensitivity of the method was found to depend on the degree of erythrocyte sensitisation. An enlargement by 40 per cent of the haemolytic halo diameter should be considered and treated as antibody rise, if the reaction was to used in serological diagnosis under the conditions tested. The findings were in agreement with the complement fixation reaction in 48 per cent of all cases and with the haemagglutination inhibition test in 53.8 per cent.
Daily variation in the udder surface temperature of dairy cows measured by infrared thermography: Potential for mastitis detection (2003)

The daily and within-day variation in udder temperature was monitored in dairy cows (n = 10) using infrared thermography (IRT). The initial assessment and prediction of udder surface temperature variation would hopefully form the basis for future development of an early detection method for mastitis. Our initial objective was to determine the magnitude and pattern of udder temperature variation. To accomplish this, we measured daily fluctuations in udder temperature and the influence of environmental factors upon these values in non-mastitic cows. Udder temperature rose significantly after an exercise period (P &lt; 0.05). Within-day monitoring of udder temperature demonstrated there was a distinct circadian rhythm. Lag regression analysis showed that previous daily udder temperatures together with environmental temperature parameters could successfully predict current udder temperature with a high degree of accuracy. The variation between predicted and actual udder temperature was within the detectable range for an inflammatory response. Infrared thermography shows promise in its application if coupled with environmental temperature monitoring as an early detection method for mastitis. Key words: Thermography, dairy cattle, environment, temperature
Exfoliation of Graphene and Assembly Formation with Alkylated‐C60: A Nanocarbon Hybrid towards Photo‐Energy Conversion Electrode Devices (2015)

An optoelectronically active assembly of graphene and alkylated-C60 is successfully constructed by direct sonication exfoliation of graphite with alkylated-C60 in organic solvents through noncovalent interactions, which exhibits around 270-fold higher photocurrent than the parent C60 derivative. This indicates that the graphene/alkylated-C60 assembly is an excellent electron accepting/charge transport material toward constructing efficient photovoltaic devices.
Transmyocardial Laser Revascularisation - A Potential Risk in Acute Situation? (2000)

Morphological and functional effects of transmyocardial laser revascularization (TMLR) are analyzed in an acute setting on a porcine model. Ten channels were drilled in the left lateral wall of the heart of 15 pigs (mean weight, 73 +/- 4 kg) with a Holmium-YAG laser (wavelength: 2.1 mu, probe diameter: 1.75 mm). Echocardiographic control was performed before the TMLR procedure as well as 5 min and 30 min thereafter. Echocardiographic parameters were recorded in short-axis at the level of the laser channels, and included left ventricular ejection fraction, fractional shortening and segmental wall motility of the channels' area (scale 0-3: 0 = normal, 1 = hypokinesia, 2 = akinesia, 3 = dyskinesia). After sacrifice the lased region was sliced perpendicularly to the channels for histological and morphometrical analysis. Five minutes after the drilling of the channels, all the echocardiographic index worsened significantly in comparison with baseline values (p < 0.01). All recovered after 30 min and showed no difference with baseline values. Cross-section of the channel lesions measured 8.8 +/- 2.4 mm2 which is more than three times that of the probe (p < 0.01). In acute conditions, the lesions due to the TMLR probe are significantly larger than the probe itself and cause a transient drop of the segmental wall motility on a healthy myocardium. These results suggest that TMLR should be used cautiously in the clinical setting for patients with an impaired ventricular function.
Internet X.509 Public Key Infrastructure Certificate Policy and Certification Practices Framework (1999)

This document presents a framework to assist the writers of certificate policies or certification practice statements for participants within public key infrastructures, such as certification authorities, policy authorities, and communities of interest that wish to rely on certificates. In particular, the framework provides a comprehensive list of topics that potentially (at the writer's discretion) need to be covered in a certificate policy or a certification practice statement. This document supersedes RFC 2527.
A phloem‐specific sucrose‐H+ symporter from Plantago major L. supports the model of apoplastic phloem loading (1994)

In this paper the cloning of a full-length cDNA clone encoding the PmSUC2 sucrose-H+ symporter from Plantago major is described. This plant allows the simple preparation of vascular bundles from the basal regions of fully developed source leaves and thus a separation of vascular and non-vascular tissue. A cDNA library was constructed from poly(A)+ RNA isolated from vascular bundles and used for the subsequent cloning of cDNAs. The respective mRNA is specifically expressed in the vascular bundles as shown on Northern blots of total RNA from vascular and non-vascular tissues. The PmSUC2 protein has 12 putative transmembrane helices and is highly homologous to other plant sucrose transporters. Substrate specificity and energy dependence of the transporter encoded by this cDNA were determined by expression in baker's yeast Saccharomyces cerevisiae. The PmSUC2 protein catalyses the transport of sucrose into transgenic yeast cells. Invertase null mutants of yeast expressing PmSUC2 accumulate sucrose more than 200-fold. This transport was sensitive to uncouplers or SH-group inhibitors. Plasma membranes from yeast cells expressing the PmSUC2 protein were purified and fused to proteoliposomes containing cytochrome-c-oxidase. In this system sucrose is accumulated only when proton motive force is generated, indicating that PmSUC2 is a sucrose-H+ symporter. The apparent molecular weight of the PmSUC2 protein is 35 kDa on 10% SDS-polyacrylamide gels. The presented data strongly support the theory of phloem loading from the apoplastic space by a sucrose-H+ symporter.
The guided tissue regeneration principle in endodontic surgery: one‐year postoperative results of large periapical lesions (1995)

Twenty patients with large endodontic lesions, which failed to respond to conventional endodontic therapy, were selected for this study. The lesions had a radiographic diameter of at least 10 mm, were removed by periradicular surgery, before retrofilling the apices with either super EBA or dessicated zinc oxide-eugenol. In 10 test sites large e-PTFE membranes (Gortex) were placed to cover the lesions, while at the control sites the lesions were not covered before resuturing. Radio-graphic analysis of the lesions at 3, 6, 9 and 12 months revealed that lesions covered with the membranes healed quicker than the control lesions, and that the quality and quantity of the regenerated bone was superior when membranes were used. Results of the study indicate that guided tissue regeneration (GTR) principles can be effectively applied to the healing of large periapical lesions, especially in through-and-through lesions.
RELEASE OF [3H]GAMMA‐AMINOBUTYRIC ACID FROM GLIAL CELLS IN RAT DORSAL ROOT GANGLIA. (1974)

Abstract— Desheathed rat dorsal root ganglia were incubated in a medium containing amino‐oxyacetic acid and [ 3 H]GABA. Under these conditions, [ 3 H]GABA is taken up exclusively by the satellite glial cells in the ganglia. Efflux of [ 3 H]GABA from the tissue was measured after passing the ganglia through a series of wash solutions. The spontaneous efflux of radioactivity, mostly [ 3 H]GABA, was more rapid in the absence of amino‐oxyacetic acid in the incubation and wash media. Raising the potassium concentration in the wash media caused an increase in the efflux of [ 3 H]GABA. This increase was sigmoidally related to the potassium concentration in the wash media, reaching a maximum at 64 m m ‐K + . The releasing effect of K + was inhibited by removing calcium from the media. Reducing the calcium and raising the magnesium concentration in the wash solutions inhibited the increased efflux of [ 3 H]GABA due to 64 m m ‐K + by 48 per cent, while 5 mM‐La 3+ and diphenylhydantoin (0·005 and 0·5 m m ) had no effect on this increase. Only a small increase in the efflux of [ 14 C]glutamate was produced by 64 m m ‐K + and it had no effect upon the effluxes of [ 3 H]glycine, [ 3 H]alanine or [ 3 H]leucine. The efflux of lactate dehydrogenase was similarly unaffected by 64 mM‐K + . The results suggest that glial cells in spinal ganglia can respond to depolarizing concentrations of potassium by releasing GABA in a calcium‐dependent process.
Polymer coating for hemoperfusion over activated charcoal (1986)

Plasma polymerization (glow discharge) was used to make a polymeric coat with hexamethyldisiloxane on the surface of activated charcoal granules. These samples were examined with ESCA and SEM for surface chemical analysis, coat thickness, and uniformity, and for changes in surface topography. The effects of the coat on absorptive capacity and release of fine particles were examined.
APF pitch-halving for 22nm logic cells using gridded design rules (2008)

The 22nm logic technology node with dimensions of ~32nm will be the first node to require some form of pitch-halving. A unique combination of a Producer APF<sup>(R)</sup>-based process sequence and GDR-based design style permits implementation of random logic functions with regular layout patterns. The APF (Advanced Patterning Film) pitch-halving approach is a classic Self-Aligned Double Patterning scheme (SADP) [1,2,3,4] which involves the creation of CVD dielectric spacers on an APF sacrificial template and using the spacers as a hardmask for line frequency doubling. The Tela Canva<sup>TM</sup> implements Gridded Design Rules (GDR) using straight lines placed on a regular grid. Logic functions can be implemented using lines on a half-pitch with gaps at selected locations.