gebhart/patrepeval huggingface dataset:
Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe. (1994)

Protein phosphatase 2C (PP2C), an Mg(2+)-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with approximately 24% identity to a rat PP2C protein. Purified Ptc1 has Mg(2+)-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.
Identification of a second tetracycline-inducible polypeptide encoded by Tn10 (1980)

Three Tn10 polypeptides were detected by analyzing the proteins synthesized in ultraviolet light-irradiated Escherichia coli cells after infection with lambda::Tn10. One of these polypeptides was the previously identified 36,000-dalton TET polypeptide. The other two had approximate sizes of 25,000 and 13,000 daltons. The syntheses of both the TET polypeptide and the 25,000-dalton polypeptide were inducible by tetracycline in lambda-immune hosts. Similarly, the synthesis of the TET polypeptide was inducible in nonimmune hosts. However, the synthesis of the 25,000-dalton polypeptide was constitutive in nonimmune hosts. An amber mutation in a gene required for tetracycline resistance on lambda::Tn10 was isolated that eliminated the synthesis of the TET polypeptide in sup+ hosts but not the synthesis of the 25,000-dalton or the 13,000-dalton polypeptides. The expression of tetracycline resistance from wild-type Tn10 was found to be anomalous in E. coli strains carrying the amber suppressors supD, supE, and supF. In general, strains containing these nonsense suppressors were less resistant to tetracycline.
Detection of colon cancer by terahertz techniques (2011)

Normal and cancer affected samples of colon tissue have been measured using transmission time-domain terahertz spectroscopy and continuous wave terahertz imaging. We show that it is possible to distinguish between normal and cancerous regions in the samples when they are fixed in formalin or embedded in paraffin. Plots of the refractive index of normal and cancer affected tissues as well as 2-D transmission THz images are shown. Experimental results will be presented and the conditions for discrimination between normal and affected tissue will be discussed.
Implementing Service Accounts for Security (2016)

This chapter discusses the general considerations for service accounts. You then examine how service accounts can be exploited. Finally, I discuss the appropriate service account strategy.
Restoring occluded regions using FW-PCA for face recognition (2012)

Occlusions in face images such as eyeglasses, hairs and whiskers decrease the performance of face recognition algorithms. Addressing this problem, this paper proposes a method for restoring occluded regions in face images. The proposed method employs Fast Weighted Principal Component Analysis (FW-PCA), which computes PCA only with effective pixels. The use of FW-PCA makes it possible to detect and restore occluded regions in face images. Through a set of experiments using public face databases, we demonstrate the effectiveness of the proposed method compared with the conventional methods.
P11.17 Splicing dysregulation drives glioblastoma malignancy: SRSF3 as a potential therapeutic target to impair glioblastoma progression (2019)

Abstract Glioblastomas (GBMs) remain the deadliest human brain tumors, with poor prognosis despite years of research. Currently, standard therapeutic strategies to treat GBM are not efficient and common survival from diagnosis is ~12–16 months. Thus, identification of new diagnostic/prognostic/therapeutic tools to tackle GBMs is crucial. Emerging evidence indicates that the cellular machinery controlling alternative splicing is altered in tumor pathologies, leading to oncogenic splicing events linked to tumor progression. Accordingly, we aimed to determine the expression pattern of the spliceosome components (SCs) and splicing factors (SFs) in high-grade astrocytomas (HGAs), mostly GBMs, and to ascertain the potential consequences of its dysregulation on GBM development. To this end, expression levels of SCs core and selected SFs were measured using a customized-microfluidic qPCR array in a well-characterized cohort of HGAs (n=33). Our results unveiled a profound alteration in the expression of multiple SCs and SFs in HGAs compared to healthy brain control-samples, wherein levels of particular elements (SRSF3/RBM22/PTBP1/RBM3) enabled perfect discrimination between non-pathological vs. tumor human-tissues, and between proneural and mesenchymal-like GBMs vs. control samples in mouse-models. Results were confirmed in an independent validation-cohort (n=49) and available Microarray dataset (Murat), which revealed that the expression of these splicing elements was correlated with relevant tumor markers and with survival. Remarkably, SRSF3/RBM22/PTBP1/RBM3 silencing (using specific siRNAs) decreased several aggressiveness parameters in vitro (e.g. proliferation, migration, tumorsphere formation, VEGFA secretion, etc.) and induced apoptosis, being SRSF3 the most relevant element affecting these parameters. Hence, a preclinical mouse model (U87MG-xenografts) with SRSF3 silencing drastically decreased in vivo tumor development/progression (i.e. tumor size, %MKI67, mitosis number, etc.) likely through a molecular/cellular mechanism involving the regulation of PDGFRB expression and its associated oncogenic signaling pathways. Overall, our results demonstrate that there is a profound dysregulation of the splicing machinery (spliceosome core and SFs) in HGAs/GBMs, which is directly associated to the development/progression of GBMs. Furthermore, this study reveals that SRSF3 can be a novel biomarker of malignancy and a potential therapeutic target to impair GBMs progression.
3β-Aminospirosolane Steroidal Alkaloids from Solanum triste (1995)

The alkaloid fraction of the MeOH extract of the aerial parts of Solanum triste afforded the new steroidal alkaloid, (22R,25R)-3 beta-amino-5-spirosolene [2], and its previously synthesized dihydro derivative, (22R,24R)-3 beta-amino-5 alpha-spirosolane [3], which is reported here for the first time as a natural product. The structures were elucidated by spectral techniques including 1H-nmr, 13C-nmr, and 1H-1H COSY, HMQC, HMBC, and NOESY nmr experiments.
Comparison of Onset Time and Magnitude of Activity for Proximal Arm Muscles and Motor Cortical Cells Before Reaching Movements (1997)

The activity of motor cortical cells and proximal arm muscles during the initiation of planar reaching movements were analyzed to identify whether features of coordinated motor patterns of muscles spanning the elbow and shoulder were evident in the discharge patterns of motor cortical cells. Shoulder and elbow muscles were divided into four groups, flexors and extensors at each joint. Features of the initial agonist activity, onset time and magnitude, at the shoulder and elbow were compared for movements in different spatial directions. As observed for human movements, differences in the onset time and the relative magnitude of electromyographic activity (EMG) of muscles acting about the shoulder and elbow were dependent on the direction of movement. Motor cortical cells were categorized as elbow or shoulder related on the basis of their response to passive movement of the joints. Differences in the onset time and the relative magnitude of activity of cells related to the shoulder and elbow were both dependent on the direction of movement and were similar to those observed for muscles spanning these joints. There was a modest, but significant correlation between the onset time and magnitude of EMG for individual muscles. A similar magnitude-time coupling was observed for individual motor cortical cells. Variations in the discharge pattern of motor cortical cells before movement that mirror those observed for muscles spanning the shoulder and elbow support the potential role of primary motor cortex in the selection, timing, and magnitude of agonist motor patterns at the shoulder and elbow to initiate reaching movements.
Semiclassical Limit of Multichannel Scattering Theory (1975)

The space-time propagator for multichannel scattering is cast in the form of a Feynman path integral where the action is a matrix and the interactions are nonlocal in time. The $S$ matrix is evaluated in the semiclassical limit, leading to trajectories which naturally follow individual channel potentials in noninteracting regions and adiabatic-like ones in strongly interacting ones. A numerical example is provided for the curve-crossing problem, and the agreement with exact quantum results is good even when the interaction region is large and/or encompasses classical turning points.
Deficiency of Myo18B in mice results in embryonic lethality with cardiac myofibrillar aberrations (2008)

Myo18B is an unconventional myosin family protein expressed predominantly in muscle cells. Although conventional myosins are known to be localized on the A-bands and function as a molecular motor for muscle contraction, Myo18B protein was localized on the Z-lines of myofibrils in striated muscles. Like Myo18A, another 18th class of myosin, the N-terminal unique domain of the protein and not the motor domain and the coiled-coil tail is critical for its localization to F-actin in myocytes. Myo18B expression was induced by myogenic differentiation through the binding of myocyte-specific enhancer factor-2 to its promoter. Deficiency of Myo18B caused an embryonic lethality in mice accompanied by disruption of myofibrillar structures in cardiac myocytes at embryonic day 10.5. Thus, Myo18B is a unique unconventional myosin that is predominantly expressed in myocytes and whose expression is essential for the development and/or maintenance of myofibrillar structure.
Calorimetric study of the interaction in aqueous solution of several uni- and bivalent metal ions with the cyclic polyether dicyclohexyl-18-crown-6 at 10,25, and 40.deg. (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCalorimetric study of the interaction in aqueous solution of several uni- and bivalent metal ions with the cyclic polyether dicyclohexyl-18-crown-6 at 10,25, and 40.deg.Reed M. Izatt, D. P. Nelson, J. H. Rytting, B. L. Haymore, and James J. ChristensenCite this: J. Am. Chem. Soc. 1971, 93, 7, 1619–1623Publication Date (Print):April 1, 1971Publication History Published online1 May 2002Published inissue 1 April 1971https://pubs.acs.org/doi/10.1021/ja00736a008https://doi.org/10.1021/ja00736a008research-articleACS PublicationsRequest reuse permissionsArticle Views356Altmetric-Citations151LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Distinct amino acid–sensing mTOR pathways regulate skeletal myogenesis (2013)

Signaling through the mammalian target of rapamycin (mTOR) in response to amino acid availability controls many cellular and developmental processes. mTOR is a master regulator of myogenic differentiation, but the pathways mediating amino acid signals in this process are not known. Here we examine the Rag GTPases and the class III phosphoinositide 3-kinase (PI3K) Vps34, two mediators of amino acid signals upstream of mTOR complex 1 (mTORC1) in cell growth regulation, for their potential involvement in myogenesis. We find that, although both Rag and Vps34 mediate amino acid activation of mTORC1 in C2C12 myoblasts, they have opposing functions in myogenic differentiation. Knockdown of RagA/B enhances, whereas overexpression of active RagB/C mutants impairs, differentiation, and this inhibitory function of Rag is mediated by mTORC1 suppression of the IRS1-PI3K-Akt pathway. On the other hand, Vps34 is required for myogenic differentiation. Amino acids activate a Vps34-phospholipase D1 (PLD1) pathway that controls the production of insulin-like growth factor II, an autocrine inducer of differentiation, through the Igf2 muscle enhancer. The product of PLD, phosphatidic acid, activates the enhancer in a rapamycin-sensitive but mTOR kinase-independent manner. Our results uncover amino acid-sensing mechanisms controlling the homeostasis of myogenesis and underline the versatility and context dependence of mTOR signaling.
X-连锁显性低血磷性佝偻病/骨软化症患者血清成纤维细胞生长因子23水平 (2019)

目的研究中国X-连锁显性低血磷性佝偻病/骨软化症(X-linked dominant hypophosphatemic rickets/osteomalacia，XLH)患者血清成纤维细胞生长因子23(fibroblast growth factor-23, FGF-23)水平特征，以及其与患者基因突变型及疾病表型严重程度的相关性。 方法收集2005至2017年就诊于北京协和医院内分泌科的XLH患者233例，所有患者均已明确致病突变。收集患者初次就诊血清，采用双抗夹心酶联免疫吸附法Elisa试剂盒进行iFGF-23水平测定。分析全段FGF-23(intact FGF-23, iFGF-23)水平在不同性别患者中的差异，及其与致病基因PHEX突变类型/位置和疾病严重程度的相关性。 结果233例XLH患者均行iFGF-23测定，患者整体iFGF-23中位水平为101.9(71.37, 143.81)ng/L，较正常参考范围(16.1~42.2 ng/L)显著升高，但XLH患者iFGF-23水平个体差异很大(6.46~542.57 ng/L)。XLH患者iFGF-23水平性别差异无统计学意义(P =0.668)，且与患者年龄亦无显著相关性(r =-0.082，P =0.254)。iFGF-23水平不受患者PHEX基因突变类型(截短/非截短突变)或突变位置(N端/C端突变, 锌离子结合域/非锌离子结合域)的影响，差异均无统计学意义(P＞0.05)。iFGF-23与患者疾病临床表型严重程度[起病年龄、血磷/正常上限比、身高(SD值)及佝偻病骨骼表现严重程度]亦无显著相关性(P＞0.05)。 结论中国XLH患者人群血清iFGF-23水平较正常人群显著升高，但个体差异较大；iFGF-23水平与患者年龄、PHEX基因突变类型或位置、患者疾病严重程度无相关性。iFGF-23可以作为明确诊断XLH疾病的一项重要指标，然而其升高程度并不能预测或代表患者疾病严重程度。
Hormonal, Lifestyle, and Dietary Factors in Relation to Leptin among Elderly Men (1999)

Serum levels of leptin may be influenced by other endocrine factors, especially testosterone and estradiol, and may be positively associated with excess fat intake independently of obesity.
Industrial Wireless Sensor Networks (2013)

Wireless Sensor Networks (WSNs) appear as a promising solution for industrial applications, especially for monitoring purposes, due to the advantages that they provide [19]. First of all, they overcome the wiring constraints present in wired industrial monitoring and control systems. Other advantages can be listed as ease of installation and maintenance, reduced cost, and better performance [50]. On the other hand, lack of standardization, strict real-timeliness, and reliability requirements of some industrial applications have limited their use in industrial domains [6]. Today, however, initial examples of industrial wireless sensor networks (IWSNs) do appear especially on process monitoring and control [28, 19], supported with standardization efforts such as IEEE 802.15.4 [2], WirelessHART [40], and ISA100.11a [25].
Numerical and experimental analysis of the correlation between EIT data eigenvalues and two-phase flow phase fraction (2019)

Abstract Electrical impedance tomography (EIT) is an imaging technique with advantages of non-intrusiveness, low-cost and high temporal resolution, which is promising for multiphase flow instrumentation. However, it produces smooth images with low spatial resolution where the interface between phases cannot be distinguished and from which the phase fraction cannot be estimated correctly. In this article, an eigenvalue analysis of EIT raw data is used to estimate the void fraction, i.e. the phase area ratio in 2D, without reconstructing images. For a given EIT sensor, each acquisition frame is represented by an impedance matrix whose eigenvalues are computed after normalization. The main characteristics of the eigenvalue distribution for different two-phase flow patterns within a cylindrical pipe are analyzed numerically. The behaviors of the leading eigenvalue and of the sum of the absolute values of the following ones are assessed as functions of the void fraction. This leads to an estimation of the two-phase flow void fraction based on the EIT sensor configuration. The presented numerical results highlight the existing correlation between the eigenvalues and the void fraction for the phase distribution patterns considered. These simulation results are compared with experimental static tests for validation.
Degradation of green light-emitting GaP:N diodes (1982)

The degradation is studied of VPE GaP:N LEDs with various stress currents. The degradation is monitored during stressing by periodically measuring the optical flux as a function of the current and the I—U characteristic. Several additional measurements are made in dependence on the degradation. These include spectral electroluminescence (300 and 77 K), minority carrier lifetime, diffusion lengths of the minority carriers, and the optical material efficiency. In the process of degradation the high material efficiency of the p-region compensates the strong decrease of the efficiency in the n-region by increasing the injection of electrons into p-region. Die Degradation an VPE GaP: N-LED's fur variierenden Laststrom wird untersucht. Durch periodische Messungen der L—1 und I—U-Kennlinie wird die Aliening in periodischen Zeitabständen festgehalten. Zusatzliche Messungen der spektralen Elektrolumineszenz (300 und 77 K), der Minoritatstragerlebensdauer, der Diffusionslänge der Minoritatsladungstrager und der Volumenlumineszenzeffjzienz dienen zur Beschreibung des Alterungsprozesses. Die höhere Volumenlumineszenzeffizienz des p-Gebietes kompensiert bei ansteigender Injektion von Elektronen in diesen Diodenbereich die starke Abnahme der Volumenlumineszenzeffizienz des n-Gebietes während der Degradation.
Fault location in meshed power networks using synchronized measurements (2010)

This paper introduces a new fault location algorithm for interconnected power networks established upon the differences of arrival times of traveling waves arriving at sparsely located synchronized recorders on any bus in the network. The performance validation of the developed method is done through an implementation on the IEEE 14-bus system by using ATP/EMTP program in collaboration with MATLAB Wavelet Toolbox.
(Invited) Important Challenges in Double Patterning Processes (2013)

Double Pattering process is one of the most promising lithography techniques for sub-40nm half-pitch technology node. Especially, Self-aligned spacer Double Patterning (SADP) has been adopted in HVM of NAND FLASH memory device[1], and it is expanding to employ in DRAM and logic device. If EUVL should not be ready on time, the industry will likely further extend DP to multiple patterning. Our proposed photo-resist core SADP has wide extendibility to Self-aligned Pitch-Tripling (SATP) and Pith-Quadrupling (SAQP) achieved 11nm hp as introduced in previous our study[2]. Sa-MP has been required to mitigate a process complexity and cost impact. Furthermore, Process variability, Pattern fidelity, CD metrology for sub 20nm pattern also has to be considered. Beside the invention of novel technical solutions, Double-patterning process is evolving steadily and its applicability is widened.
Multiparameter persistent homology landscapes identify immune cell spatial patterns in tumors (2021)

Highly resolved spatial data of complex systems encode rich and nonlinear information. Quantification of heterogeneous and noisy data-often with outliers, artifacts, and mislabeled points-such as those from tissues, remains a challenge. The mathematical field that extracts information from the shape of data, topological data analysis (TDA), has expanded its capability for analyzing real-world datasets in recent years by extending theory, statistics, and computation. An extension to the standard theory to handle heterogeneous data is multiparameter persistent homology (MPH). Here we provide an application of MPH landscapes, a statistical tool with theoretical underpinnings. MPH landscapes, computed for (noisy) data from agent-based model simulations of immune cells infiltrating into a spheroid, are shown to surpass existing spatial statistics and one-parameter persistent homology. We then apply MPH landscapes to study immune cell location in digital histology images from head and neck cancer. We quantify intratumoral immune cells and find that infiltrating regulatory T cells have more prominent voids in their spatial patterns than macrophages. Finally, we consider how TDA can integrate and interrogate data of different types and scales, e.g., immune cell locations and regions with differing levels of oxygenation. This work highlights the power of MPH landscapes for quantifying, characterizing, and comparing features within the tumor microenvironment in synthetic and real datasets.
Sleep and aging (2018)

This review describes normal and disordered sleep in the older adult population. Although several distinctive sleep changes across the lifespan are normative, including changes in the amount of time spent in different stages of sleep and a shift in the timing of circadian rhythms, sleep disorders are also common in late life. Sleep-disordered breathing, insomnia, circadian rhythm sleep-wake disorders, and parasomnias occur frequently in older adults and contribute to overall higher rates of poor sleep with advanced age. Assessment and treatment of sleep disorders has been shown to improve functioning and quality of life in older adults; however, the process of diagnosis and intervention is often complicated due to the presence of multiple medical comorbidities, medication side effects, and specific age-related risk factors for sleep disruption. Additional challenges to recognizing, diagnosing, and treating sleep disorders in older adults with dementia and those in long-term care facilities also exist, further complicating the clinical management of sleep disorders in these patients.
Electrically independent subcircuits for a seven-junction spectrum splitting photovoltaic module (2014)

An electrical system that facilitates independent electrical connection of subcells with different bandgaps is required in order to optimally extract two-terminal power generated by spectrum-splitting photovoltaics. We have designed such a system for a seven subcell spectrum-splitting module using commercially available electrical components. Modified detailed balance modeling of the subcells coupled with HSPICE circuit simulation results in 87.5% power-weighted average subcell-to-grid electrical efficiency. Additional analysis indicates electrical efficiencies in excess of 95% are possible with custom power conditioning components and appropriately designed contacts.