gebhart/patrepeval huggingface dataset:
Aromatic Fluorine Compounds. II. Synthesis of p-Fluorophenol by the Selective Hydrolysis of p-Bromofluorobenzene1 (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAromatic Fluorine Compounds. II. Synthesis of p-Fluorophenol by the Selective Hydrolysis of p-Bromofluorobenzene1MAX M. BOUDAKIAN, ROBERT J. EBER, W. EDWARD KUEHLEWIND Jr., and RICHARD E. McARTHURCite this: J. Org. Chem. 1961, 26, 11, 4641–4645Publication Date (Print):November 1, 1961Publication History Published online1 May 2002Published inissue 1 November 1961https://pubs.acs.org/doi/10.1021/jo01069a107https://doi.org/10.1021/jo01069a107research-articleACS PublicationsRequest reuse permissionsArticle Views147Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Abstract P482: Elucidating And Characterizing The Molecular Mechanistic Role Of Phospholamban L39 Stop In The Pathophysiology Of Cardiomyopathy Using Patient-derived Human Induced Pluripotent Stem Cells And Humanized Knock-in Mouse Model Systems (2021)

Background: Heart failure (HF) is a complex clinical condition associated with substantial morbidity and mortality worldwide. The contractile dysfunction and arrhythmogenesis related to HF has been linked to the remodelling of calcium (Ca ++ ) handling. Phospholamban (PLN) has emerged as a key regulator of intracellular Ca ++ concentration. Of the PLN mutations, L39X is intriguing as it has not been fully characterized. This mutation is believed to be functionally equivalent to PLN null (KO) but contrary to PLN KO mice, L39X carriers develop a lethal cardiomyopathy (CMP). Our study aims at using induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs) from homozygous L39X carriers to elucidate the role of L39X in human pathophysiology. Our plan also involves the characterization of humanized L39X knock-in mice (KM), which we hypothesize will develop a CMP from mis-localization of PLN and disruption of Ca ++ signalling. Methodology and Results: Mononuclear cells from Hom L39X carriers were obtained to generate 11 integration-free patient-specific iPSC clones. The iPSC-CMs were derived using established protocols. Compared to the WT iPSC-CMs, the Hom L39X derived-CMs PLN had an abnormal cytoplasmic distribution and formed intracellular aggregates, with the loss of perinuclear localization. There was also a 70% and 50% reduction of mRNA and protein expression of PLN respectively in L39X compared to WT iPSC-CMs. These findings indicated that L39X PLN is both under-expressed and mis-localized within the cell. To validate this observation in-vivo, we genetically modified FVB mice to harbour the human L39X. Following electroporation, positively transfected mouse embryonic stem cells were injected into host blastocysts to make humanized KM that were subsequently used to generate either a protamine-Cre (endogenous PLN driven expression) or a cardiac TNT mouse (i.e., CMP specific). Conclusion: Our data confirm an abnormal intracellular distribution of PLN, with the loss of perinuclear accumulation and mis-localization, suggestive of ineffective targeting to or retention of L39X. The mouse model will be critically important to validate the in-vitro observations and provides an ideal platform for future studies centred on the development of novel therapeutic strategies including virally delivered CRISPR/Cas9 for in-vivo gene editing and testing of biochemical signalling pathways.
Abstract 3687: Dot1l inhibition selectively impairs androgen receptor dependent prostate cancer growth through loss of telomere integrity (2018)

Abstract The histone methyltransferase DOT1L methylates lysine 79 (K79) on histone H3 and this histone mark is associated with activation of gene expression. DOT1L has a well-known role in MLL fusion leukemogenesis, however, its role in prostate cancer is yet to be elucidated. Here we show that DOT1L is overexpressed in many solid tumors including prostate cancer and is associated with a poor outcome. Genetic and chemical targeting of DOT1L showed a preferential loss in cell viability and colony formation in androgen receptor (AR)-signaling competent prostate cancer cells, including castration-resistant and enzalutamide-resistant cells. DOT1L inhibition reduced AR levels and upregulated enzymes involved in testosterone catabolism but only affected the expression of a subset of AR target genes. More importantly, loss of DOT1L selectively impaired telomere integrity by promoting telomere uncapping and shortening in AR-signaling competent cells. DOT1L inhibition also reduced the expression of telomerase and Shelterin complex genes including TRF2 and POT1. Recruitment of AR to telomeres and K79 methylation at sub-telomeric regions was also diminished. Consequently, loss of DOT1L leads to induction of Telomere dysfunction induced foci (TIF) and activates a cellular senescence program in these cells. Hence, these results provide compelling evidence for DOT1L inhibition as a novel therapeutic approach in advanced prostate cancers. Citation Format: Rajita Vatapalli, Yara Rodriguez, Changsheng Zhao, Vinay Sagar, Jonathan Anker, Sahithi Pamarthi, Kenji Unno, Benedito Carneiro, Debabrata Chakravarti, Sarki Abdulkadir. Dot1l inhibition selectively impairs androgen receptor dependent prostate cancer growth through loss of telomere integrity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3687.
Sodium, Potassium, the Sympathetic Nervous System, and the Renin—Angiotensin System (2001)

Under the usual circumstances of everyday life, the phasing of human circadian clocks and rhythms is set, or synchronized, by the sleep-in-darkness— activity-in-light, 24-h routine. These time cues greatly influence the intrinsic diurnal rhythm for blood pressure (BP). As an example of this, when shift workers are assigned to night duty, they ordinarily adhere to a different sleep—activity routine than do day workers. Because of this, the timing of their peak and trough BP differs, with reference to external clocktime, if compared to daytime active individuals. The biologic time structure of man is an inherited characteristic for a number of parameters, such as BP. Its normal expression, however, may be influenced by either environmental/nutritional factors or an individual's normal or pathophysiologically acquired neuro-humoral status. When normal phase relationships change between circadian bioperiodicities, BP patterns may alter radically and unpredictably. The purpose of this review is to characterize the neurohumoral and nutritional determinants of the ambulatory blood pressure (ABP) profile in normotensive and hypertensive patients. In particular, this review focuses on the sympathetic nervous system (SNS), the renin—angiotensin—aldosterone (RAA) axis, and the role of dietary sodium (Na+) and potassium (K+) in shaping circadian BP patterns.KeywordsAtrial Natriuretic PeptideAmbulatory Blood PressureAmbulatory Blood Pressure MonitoringCircadian VariationSympathetic Nervous System ActivityThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Rett syndrome with and without detected MECP2 mutations: An attempt to redefine phenotypes (2010)

A clear regressive period (with loss of prehension and language, deceleration of growth) and the presence of more than three different stereotypies, rigidity and ataxic-rigid gait seemed to be very helpful in differentiating Group I from Group II.
Kinematics of successful knee prostheses during weight‐bearing: Three‐dimensional movements and positions of screw axes in the Tricon‐M and Miller‐Galante designs (1994)

Using roentgen stereophotogrammetric analysis we recorded the three-dimensional movements in six knees with implanted Tricon-M prostheses and ten knees with Miller-Galante prostheses as the patients ascended a platform. Fourteen patients with normal knees were used as controls. The two prosthetic designs displayed decreased internal tibial rotation and the Tricon-M increased valgus rotation. A central point on the tibial articular surface had a more lateral position in the Tricon-M design and a more distal one in the Miller-Galante design compared to normal knees. Increased posterior displacement with increasing flexion was observed in both designs. When the normal knees were extended at full weight-bearing the helical axes mainly shifted inclination in the frontal plane. In the prosthetic knees there was a tendency to anterior-posterior displacement of the axes as extension proceeded, especially in the Miller-Galante design. Translations along the helical axes were larger than normal in the Miller-Galante and smaller in the Tricon-M knees, reflecting differences in constraint of the two designs.
Applications of Support Vector Machines in Chemistry (2007)

This chapter contains sections titled: Introduction A Nonmathematical Introduction to SVM Pattern Classification The Vapnik–Chervonenkis Dimension Pattern Classification with Linear Support Vector Machines Nonlinear Support Vector Machines SVM Regression Optimizing the SVM Model Practical Aspects of SVM Classification Practical Aspects of SVM Regression Review of SVM Applications in Chemistry SVM Resources on the Web SVM Software Conclusions References
Amino acid metabolism of myeloma cells in culture (1976)

The growth of myeloma cells in Leibovitz medium supplemented with 20% serum was limited by the depletion of glutamine. A simple modification of the Leibovitz medium by increasing the concentrations of glutamine, lysine, isoleucine, leucine, sodium pyruvate, galactose, and vitamins resulted in over 100% increase in cell growth yield. The total myeloma protein produced by the cells was increased by approximately 90% in modified Leibovitz media. Analysis of spent culture media for 19 amino acids showed that the concentrations of 8 amino acids were reduced; those of 5 amino acids were increased and the other 6 did not change significantly.
Novel Approaches and Hurdles to Somatic Cloning in Cattle (2002)

The overall efficiency of somatic cloning in cattle is still low. Many factors are necessary for successful birth of live offspring. Among them, the source of donor cells reveals the importance of the donor genotype but also the influence of the cell line itself. The cell cycle stage has been intensively investigated, and recent results indicate that, in cattle, the G0 stage of the donor nuclei is not a prerequisite for reprogramming, as highly proliferating cultured fibroblasts also result in live offspring after nuclear transfer. A technical approach using direct microinjection of fibroblast nuclei, instead of fusion of the whole cell, has proved to result in high in vitro development rates in cattle. However, full-term development of somatic cloned embryos is still limited by long-lasting effects and a high incidence of losses at periimplantation time (as well as in late gestation and around calving).
Properties of encapsulated CIGS cells in 85&#x00B0;C/85%RH (2008)

This paper concerns studies of encapsulated cells subjected to an environment of 85°C and 85%RH (85/85). Cells are encapsulated with PNNL multi-layer coatings (referred to as PML coatings) utilizing alternating layers of Al <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> O <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> , and an advanced polymer. The new polymer has been determined to withstand the 85/85 environment. Two types of cells were used for these studies, namely, SSI mini-modules (which are actually CIGSS devices) and CIGS cells provided by the Institute of Energy Conversion (IEC). Cells were coated and stressed at 85/85 in an environmental chamber. Current-voltage characteristics were acquired before and after coating, and periodically after being subjected to the 85/85 environment. Whereas coated SSI modules were determined to last 1000 hours when stressed at 60°C/90%RH without degradation, the efficiency of these modules degrade to a level of 60% of the beginning-of-life value when stressed at 85/85. Encapsulated IEC cells, however, have exhibited extraordinary results. The efficiency of several encapsulated cells did not decrease for 1500 hours in an 85°C/85%RH environment. This results establishes a benchmark for stressed, encapsulated CIGS cells.
Infant Temperament and High-Risk Environment Relate to Behavior Problems and Language in Toddlers (2011)

CHILDREN growing up in adverse social environments had increased behavioral problems and compromised language development. Conversely, an easy temperament acts as a protective factor for social-emotional development and could be related to resilience.
Compressive light field imaging (2010)

Light field imagers such as the plenoptic and the integral imagers inherently measure projections of the four dimensional (4D) light field scalar function onto a two dimensional sensor and therefore, suffer from a spatial vs. angular resolution trade-off. Programmable light field imagers, proposed recently, overcome this spatioangular resolution trade-off and allow high-resolution capture of the (4D) light field function with multiple measurements at the cost of a longer exposure time. However, these light field imagers do not exploit the spatio-angular correlations inherent in the light fields of natural scenes and thus result in photon-inefficient measurements. Here, we describe two architectures for compressive light field imaging that require relatively few photon-efficient measurements to obtain a high-resolution estimate of the light field while reducing the overall exposure time. Our simulation study shows that, compressive light field imagers using the principal component (PC) measurement basis require four times fewer measurements and three times shorter exposure time compared to a conventional light field imager in order to achieve an equivalent light field reconstruction quality.
METC Gasifier Advanced Simulation (MGAS) model (1992)

Morgantown Energy Technology Center is developing an advanced moving-bed gasifier, which is the centerpiece of the Integrated Gasifier Combined-Cycle (IGCC) system, with the features of good efficiency, low cost, and minimal environmental impact. A mathematical model of the gasifier, the METC-Gasifier Advanced Simulation (MGAS) model, has been developed for the analysis and design of advanced gasifiers and other moving-bed gasifiers. This report contains the technical and the user manuals of the MGAS model. The MGAS model can describe the transient operation of coflow, counterflow, or fixed-bed gasifiers. It is a one-dimensional model and can simulate the addition and withdrawal of gas and solids at multiple locations in the bed, a feature essential for simulating beds with recycle. The model describes the reactor in terms of a gas phase and a solids (coal or char) phase. These phases may exist at different temperatures. The model considers several combustion, gasification, and initial stage reactions. The model consists of a set of mass balances for 14 gas species and three coal (pseudo-) species and energy balances for the gas and the solids phases. The resulting partial differential equations are solved using a finite difference technique.
Tsunami early warning and decision support (2010)

Abstract. An innovative newly developed modular and standards based Decision Support System (DSS) is presented which forms part of the German Indonesian Tsunami Early Warning System (GITEWS). The GITEWS project stems from the effort to implement an effective and efficient Tsunami Early Warning and Mitigation System for the coast of Indonesia facing the Sunda Arc along the islands of Sumatra, Java and Bali. The geological setting along an active continental margin which is very close to densely populated areas is a particularly difficult one to cope with, because potential tsunamis' travel times are thus inherently short. National policies require an initial warning to be issued within the first five minutes after an earthquake has occurred. There is an urgent requirement for an end-to-end solution where the decision support takes the entire warning chain into account. The system of choice is based on pre-computed scenario simulations and rule-based decision support which is delivered to the decision maker through a sophisticated graphical user interface (GUI) using information fusion and fast information aggregation to create situational awareness in the shortest time possible. The system also contains risk and vulnerability information which was designed with the far end of the warning chain in mind – it enables the decision maker to base his acceptance (or refusal) of the supported decision also on regionally differentiated risk and vulnerability information (see Strunz et al., 2010). While the system strives to provide a warning as quickly as possible, it is not in its proper responsibility to send and disseminate the warning to the recipients. The DSS only broadcasts its messages to a dissemination system (and possibly any other dissemination system) which is operated under the responsibility of BMKG – the meteorological, climatological and geophysical service of Indonesia – which also hosts the tsunami early warning center. The system is to be seen as one step towards the development of a "system of systems" enabling all countries around the Indian Ocean to have such early warning systems in place. It is within the responsibility of the UNESCO Intergovernmental Oceonographic Commission (IOC) and in particular its Intergovernmental Coordinating Group (ICG) to coordinate and give recommendations for such a development. Therefore the Decision Support System presented here is designed to be modular, extensible and interoperable (Raape et al., 2010).
Evaluation of NOx emissions from advanced-technology range burners. Final report, March 1, 1990-June 30, 1991 (1991)

With growing concern for indoor air quality, it was important that emission rates from unvented combustion sources be cataloged to evaluate the effect they may have on the indoor air quality. Flue gas emissions were evaluated from new or European type or Japanese sealed or not sealed blue flame type range top burners installed in a conventional free standing gas range. Emissions were also evaluated from burners in advanced technology market ranges equipped with either blue flame or infrared burners.
Wireless-powered cooperative energy aware anycast routing in wireless sensor networks (2016)

A wireless-powered cooperative energy aware anycast routing protocol is proposed in this work. In contrary to conventional cooperative networks, it is considered here that all the relays did not have embedded energy supply, rather equipped with rechargeable batteries and energy harvesting units. Hence, from source signals, they accumulate sufficient harvested energy before the information is forwarded to its destination. Each relay between two basic modes will switch adaptively, which are information forwarding and energy harvesting. The research is limited to decode-and-forward scheme and fixed ratio combining, which works at the relay node of the network and the receiver end, respectively. The physical layer cooperative diversity and network layer multi-hop routing lead us to devise a minimum energy routing protocol as the joint optimization of power required for transmission at physical layer and also at network layer for the process to select a link. Simulating our algorithm demonstrates that the suggested cooperative energy aware anycast routing scheme has a better end-to-end delay and better ratio of packet delivery. Results further reveal that our proposed algorithm has improved energy consumption in comparison to the non-cooperative energy aware anycast scheme and the cooperation-based robust cooperative routing protocol scheme. Sensed data are allocated between nearby nodes by cooperative energy aware anycast routing in a cost-effective way in order to achieve maximum network lifetime.
C-Terminal Repeats ofClostridium difficileToxin A Induce Production of Chemokine and Adhesion Molecules in Endothelial Cells and Promote Migration of Leukocytes (2007)

The C-terminal repeating sequences of Clostridium difficile toxin A (designated ARU) are homologous to the carbohydrate-binding domain of streptococcal glucosyltransferases (GTFs) that were recently identified as potent modulins. To test the hypothesis that ARU might exert a similar biological activity on endothelial cells, recombinant ARU (rARU), which was noncytotoxic to cell cultures, was analyzed using human umbilical vein endothelial cells. The rARU could bind directly to endothelial cells in a serum- and calcium-dependent manner and induce the production of interleukin-6 (IL-6), IL-8, and monocyte chemoattractant protein 1 in a dose-dependent manner. An oligosaccharide binding assay indicated that rARU, but not GTFC, binds preferentially to Lewis antigens and 3'HSO3-containing oligosaccharides. Binding of rARU to human endothelial or intestinal cells correlated directly with the expression of Lewis Y antigen. Bound rARU directly activated mitogen-activated protein kinases and the NF-kappaB signaling pathway in endothelial cells to release biologically active chemokines and adhesion molecules that promoted migration in a transwell assay and the adherence of polymorphonuclear and mononuclear cells to the endothelial cells. These results suggest that ARU may bind to multiple carbohydrate motifs to exert its biological activity on human endothelial cells.