gebhart/patrepeval huggingface dataset:
Plasmapheresis treatment of antibody‐mediated rejection in an A2 donor to O pediatric liver transplant recipient (2009)

It is safe to transplant kidneys from blood group A2 donors into O recipients if the latter have low titers of anti-A antibodies. However, in liver transplantation, O and B recipients of A2 donor livers are not routinely screened for anti-blood group antibodies because of the immuno-absorptive capacity of the liver and the low incidence of antibody-mediated rejection. Herein, we report a rare case of combined cell and antibody-mediated rejection in a pediatric blood group O recipient of an A2 donor liver, and rescue of the allograft using PP and IVIG.
Methodology of ECG Interpretation in the Lyon Program (1990)

To provide an in-depth interpretation of the spatial QRS-T contour, we established a set of vectorcardiographic parameters computed octant by octant. For each statement the criteria constitute a model which is closely related to pathophysiological patterns. The diagnostic strategy is of the heuristic type. For each diagnosis a table lists several criteria providing both specific and differential diagnostic information. Inter-table competition is handled by specific logic. The program classifies diagnoses according to the number of non-satisfied criteria. Three versions are available including 124, 51 and 7 diagnoses, respectively. The first, intended for interpretation of complex situations, has given satisfactory results in such situations. Rhythm analysis is performed from 8-second recordings. All functions--acquisition, quality control, choice of a dominant complex, wave recognition, parameter extraction, interpretation, editing, storage--are integrated into a mobile cart. A complementary program performs serial analysis of successive tracings. It highlights with great precision morphological changes in the spatial loops and interval variations.
Formation of Dynamic Duolayer Systems at the Air/Water Interface by using Non-ionic Hydrophilic Polymers (2013)

The inclusion of a water-soluble polymer, poly(vinyl pyrrolidone) (PVP), into a surface active film composition before application to the water surface leads to the formation of a dynamic duolayer; a novel surface film system. This duolayer shows improved surface viscosity over the monolayer compound alone, while the addition of polymer maintains other film properties such as evaporation control and equilibrium spreading pressure. Brewster Angle Microscopy shows that the duolayer film undergoes a different formation mechanism upon film compression, and the resultant surface pressure/area isotherm is different at lower surface pressures indicating the PVP is present on the water surface at these pressures and squeezed out to the water subphase at higher pressures. The addition of water-soluble polymers to form a dynamic duolayer provides a unique way to produce defect-free and tightly packed films while polymer is associated with the film. This finding provides new knowledge for the design of surface films with improved properties with potential applications in many areas.
Matrix Extension Study: Validation of the ANSR®Salmonella Method for Detection of Salmonella spp. in Pasteurized Egg Products (2014)

A matrix extension study was conducted to validate the ANSR(®) Salmonella method for use with pasteurized egg products. Four diverse egg product types were tested by the ANSR method and the U.S. Department of Agriculture's Microbiology Laboratory Guidebook reference culture procedure. There were no significant differences in the number of positive test portions by the two methods for any of the products examined. Independent laboratory testing of pasteurized liquid egg also found no significant difference in performance between the ANSR and reference culture procedures. There were no false-positive results obtained in either internal or independent laboratory testing. Inclusivity testing using a new enrichment medium specifically designed for use with pasteurized egg products produced 112 positive results out of 113 Salmonella spp. strains tested, with only a single strain of S. Weslaco testing negative by the ANSR assay. Exclusivity testing of 38 non-salmonellae produced all negative ANSR results. It is concluded that ANSR Salmonella is a reliable, sensitive, and specific method for detection of Salmonella spp. in pasteurized egg products.
Construction and characterisation of a recombinant fowlpox virus that expresses the human papilloma virus L1 protein (2011)

This FPL1 recombinant represents an appropriate immunogen for expression of HPV-L1 in human cells. The final aim is to develop a safe, immunogenic, and less expensive prophylactic vaccine against HPV.
ChemInform Abstract: Solid‐State Photochemistry and Structure of trans‐(Et3P)2Ni(N3)2: Photodeposition of Nickel. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Glutathione aerosol suppresses lung epithelial surface inflammatory cell-derived oxidants in cystic fibrosis (1999)

Cystic fibrosis (CF) is characterized by accumulation of activated neutrophils and macrophages on the respiratory epithelial surface (RES); these cells release toxic oxidants, which contribute to the marked epithelial derangements seen in CF. These deleterious consequences are magnified, since reduced glutathione (GSH), an antioxidant present in high concentrations in normal respiratory epithelial lining fluid (ELF), is deficient in CF ELF. To evaluate the feasibility of increasing ELF GSH levels and enhancing RES antioxidant protection, GSH aerosol was delivered (600 mg twice daily for 3 days) to seven patients with CF. ELF total, reduced, and oxidized GSH increased (P < 0.05, all compared with before GSH therapy), suggesting adequate RES delivery and utilization of GSH. Phorbol 12-myristate 13-acetate-stimulated superoxide anion (O2-.) release by ELF inflammatory cells decreased after GSH therapy (P < 0.002). This paralleled observations that GSH added in vitro to CF ELF inflammatory cells suppressed O2-. release (P < 0.001). No adverse effects were noted during treatment. Together, these observations demonstrate the feasibility of using GSH aerosol to restore RES oxidant-antioxidant balance in CF and support the rationale for further clinical evaluation.
ФОТОЭЛЕКТРИЧЕСКИЕ И ФОТОРЕФРАКТИВНЫЕ СВОЙСТВА КОМПОЗИТОВ НА ОСНОВЕ ПОЛИВИНИЛКАРБАЗОЛА И ТЕТРА-15-КРАУН-5-ФТАЛОЦИАНИНАТА РУТЕНИЯ(II) С АКСИАЛЬНО КООРДИНИРОВАННЫМИ МОЛЕКУЛАМИ ПИРАЗИНА (2012)

Изучены фотоэлектрические и фоторефрактивные характеристики композитов на основе поливинилкарбазола, содержащих краунзамещенный фталоцианинат рутения с аксиально координированными молекулами пиразина (R4Pc)Ru(pyz)2 (R4Pc2= [4,5,4’,5’,4’’,5’’,4’’’,5-тетракис-(1,4,7,10,13-пентаоксатридекаметилен) фталоцианинат-ион], pyz  пиразин). Супрамолекулярные ансамбли этих комплексов ответственны за оптическое поглощение, фотоэлектрическую и фоторефрактивную чувствительность в ближней ИК-области. Установлено при действии лазера 1064 нм, что квантовая эффективность образования подвижных носителей заряда, оцененная по фототоку, соответствует уравнению Онзагера, когда независимо от содержания (R4Pc)Ru(pyz)2 квантовый выход образования термализованных электрон-дырочных пар 0 = 0.35 и расстояние разделения 9.8 A. Кинетические кривые усиления информационного лазерного луча имеют колоколообразную форму. Это дает основание предполагать, что фоторефрактивные характеристики занижены из-за снижения до нулевого значения поля Е0 внутри слоя в результате накопления объемного заряда в приэлектродном пространстве при прохождении темнового тока. Оцененный по максимуму колоколообразной кривой коэффициент двухлучевого усиления при содержании (R4Pc)Ru(pyz)2 7 мас. % составляет = 62 см-1.
The effect of domestic air treatment equipment on the concentration of radon-222 daughters in a sealed room (1980)

REDUCTION OF the ventilation rate in houses in the interests of energy conservation can lead to a build-up of radon-222 in room air. This results in irradiation of the lungs by the radioactive daughters of radon. An electrostatic precipitator has been used to remove radon daughters from the atmosphere of a sealed room. It is shown that this can reduce the dose to the bronchial epithelium by an order of magnitude, and effectively counter the effects even of extremely low ventilation rates.
Thiol-Organosilica Particles Internally Functionalized with Propidium Iodide as a Multicolor Fluorescence and X-ray Computed Tomography Probe and Application for Non-Invasive Functional Gastrointestinal Tract Imaging (2012)

Propidium iodide (PI) is a fluorescent nucleic acid dye that contains iodide molecules. Thiol-organosilica particles that were internally functionalized with PI (PI particles) were prepared for X-ray computed tomography (CT) and multicolor fluorescence imaging. PI particles of various sizes were synthesized in a one-pot process. The particles showed unique fluorescent signals and X-ray absorption, with enhancement of the fluorescence intensity of PI located inside organosilica particles. PI particles had multicolor fluorescence, including original fluorescence and near-infrared (NIR). Orally administered PI particles were observed in the gastrointestinal tract (GIT) using fluorescent imaging devices and X-ray CT. An in vivo fluorescence imaging system could detect the NIR fluorescence of PI particles in the GIT specifically. Multipurpose zoom fluorescence microscopy was used to noninvasively visualize the real-time passage of particles and the movement of the GIT. The passage and distribution of particles over time in the GIT were demonstrated using X-ray CT. A correlation analysis between the fluorescent and X-ray CT data demonstrated the characteristics, limitations, and novel potential for noninvasive functional GIT dual modal imaging.
Chromosome-scale genome assembly of kiwifruit Actinidia eriantha with single-molecule sequencing and chromatin interaction mapping v2 (2018)

This protocol includes a computational pipeline used in assembly and annotation of Kiwifruit Actinidia eriantha genome.
Young hearts are as susceptible as old hearts to the direct and acute cardiotoxic effects of ultrafine air pollutants (2007)

Environmental exposure to air pollutants triggers cardiac events and increases cardiac morbidity and mortality in the general population. We hypothesized that age might become a key factor in cardiotoxic effects triggered by air pollutants. Experiments were carried out in Langendorff‐perfused hearts obtained from young (4 month old) and aged (26 months old) Fisher/Brown Norway rats. Standard diesel particulate matter (National Institute of Standards and Technology) was filtered for ultrafine particles (UP; &lt;0.1 μ) and suspended in perfusion buffer. Four groups of hearts were studied: young and old control buffer‐perfused hearts (YC, OC), and young and old hearts perfused for 30 min with UP (YUP, OUP). Control hearts demonstrated a slight decrease in developed pressure by17% and 8% in YC and OC groups (from 122±9 to 102±13 mmHg and from 133±7 to 123±9 mmHg respectively; p&lt;0.05 vs. baseline), while UP perfused hearts showed a dramatic fall by 33% and 35% in YUP and OUP groups (p&lt;0.05 vs. control). Positive dP/dt remained stable in both control groups, but was decreased by 28% in YUP and OUP groups (p&lt;0.05 vs. control). Coronary flow was significantly decreased in both UP groups (by 28% in YUP and by 26% in OUP groups; p&lt;0.05 vs. control). These results indicate that both young and old hearts were equally susceptible to direct and acute cardiotoxic effects of ultrafine air pollutants. Supported by US EPA Grant No. RD‐83195201.
Size Selective Self-Sorting in Coordination-Driven Self-Assembly of Finite Ensembles (2008)

Size selective self-sorting in the coordination-driven self-assembly of two-dimensional (2-D) polygons and three-dimensional (3-D) cages is presented. Two types of polygons (rectangular and triangular) of different size are formed spontaneously from within mixtures of a molecular "clip" or a 60 degrees organoplatinum acceptor with dipyridyl linkers of different lengths via self-sorting. Furthermore, two different sized 3-D supramolecular cages are formed upon mixing one ditopic organoplatinum acceptor and two different sized tritopic donors from the self-sorting process. The formation of these polygons and cages is characterized using NMR spectroscopy and electrospray ionization mass spectrometry (ESI-MS). In all cases, the self-sorting process is directed by the size of the donor building blocks and a dynamic, thermodynamically driven self-correction process resulting in the formation of discrete products from complex mixtures.
POROUS SUBSTRUCTURE OF THE GLOMERULAR SLIT DIAPHRAGM IN THE RAT AND MOUSE (1974)

The highly ordered, isoporous substructure of the glomerular slit diaphragm was revealed in rat and mouse kidneys fixed by perfusion with tannic acid and glutaraldehyde. The slit diaphragm was similar in both animal species and appeared as a continuous junctional band, 300-450 A wide, consistently present within all slits formed by the epithelial foot processes. The diaphragm exhibited a zipper-like substructure with alternating, periodic cross bridges extending from the podocyte plasma membranes to a central filament which ran parallel to and equidistant from the cell membranes. The dimensions and spacing of the cross bridges defined a uniform population of rectangular pores approximately 40 by 140 A in cross section and 70 A in length. The total area of the pores was calculated to be about 2-3% of the total surface area of the glomerular capillaries. Physiological data indicate that the glomerular filter functions as if it were an isoporous membrane which excludes proteins larger than serum albumin. The similarity between the dimensions of the pores in the slit diaphragm and estimates for the size and shape of serum albumin supports the conclusion from tracer experiments that the slit diaphragm may serve as the principal filtration barrier to plasma proteins in the kidney.
Flexible Cu(In,Ga)Se2 Solar Cells with Absorber Thickness of 1 µm Deposited at Higher Evaporation Rates (2013)

Use of flexible substrates, growth of thinner absorbers or growth at higher deposition rates are suitable strategies to increase the manufacturing throughput of Cu(In,Ga)Se2-based photovoltaics. In this work these 3 aspects are combined and the influence of increased growth rate for thinner absorbers (0.8-0.9 μm) deposited on flexible polyimide foils is investigated. Our low temperature multi-stage co-evaporation process is modified to grow CIGS absorber layers with about 1/3 of the standard thickness leading to a total deposition time of 20 min. Evaporation rates are increased separately (up to 4 times the standard rate) in each stage while they are kept constant in the other stages. The microstructure is not affected significantly by the increase in growth rate during any stage. No decrease in performance is observed with increased deposition rate in the 1st and 3rd stage. Improved photovoltaic parameters are measured for the cell with the absorber grown at a higher rate during the 2nd stage, leading to an increase in conversion efficiency from 13.7% up to 15.1% compared to an absorber grown with standard evaporation rates. Modifications of the absorber microstructure and electronic quality are discussed to explain the differences in final solar cell performance.
[Study and preparation of Vi-PHA reagent and its application for detection of Salmonella typhi carriers]. (1992)

Purified S. typhi Vi antigen is sensitized with equal volume of tannic acid treated formalational sheep erythrocytes (SRBC) at a final concentration of 1 microgram/ml. The Vi-passive hemagglutination assay (Vi-PHA) diagnostic reagent is developed to detect Vi antibodies to S. typhi for the detection of chronic carriers after typhoid fever and the screening S. typhi healthy carriers from food-handlers, which is characterized with high sensitivity, strong specificity and good stability. This Vi-PHA reagent is able to detect 1.16 micrograms/ml of Vi antibodies and doesn't make any cross reaction with healthy sera. For the sera of other diseases, the cross rate is only 0.84%. Using this reagent, 19 positive sera (6.93%) are detected from 274 convalescent sera from typhoid fever, 14 convalescents of which are stool-culture S. typhi positive, that persists a positive rate of 73.68%; 3 positive sera are detected from 106 foodhandlers, one of which is stool-culture S. typhi positive. Therefore, the reagent is simple, convenient, rapid and easy to be applicated in basic unit.
The effect of stochastic electrical noise on hard-to-heal wounds (2010)

This study was supported by Vivisol (Italy), a distributor of Lifewave's BST.
Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase. (1988)

The light-activated NADP-malate dehydrogenase (NADP-MDH) catalyzes the reduction of oxaloacetate to malate in higher plant chloroplasts. This enzyme is regulated in vivo by the ferredoxin-thioredoxin system through redox reactions. NADP-MDH has been photoactivated in vitro in a chloroplast system reconstituted from the pure protein components and thylakoid membranes. Photoactivation was accompanied by the appearance of new thiol groups (followed by [14C]iodoacetate incorporation). 14C-Carboxymethylated NADP-MDH has been purified from the incubation mixture and its amino-terminal sequence analyzed. Two [14C]carboxymethylcysteines were identified at positions 10 and 15 after light activation, while they were not detected in the dark-treated protein. In addition, the analysis of the tryptic digest of light-activated [14C]carboxymethylated NADP-MDH revealed that the radioactive label was mostly incorporated in Cys10 and Cys15, indicating that these 2 residues play a major role in the light activation mechanism. Moreover, an activation model, in which photoreduced thio-redoxin was replaced by the dithiol reductant dithio-threitol, has been developed. When NADP-MDH was activated in this way, the same sulfhydryls were found to be labeled, and alternatively, they did not incorporate any radioactivity when dithiothreitol reduction was performed after carboxymethylation in denaturating conditions. These results indicate that activation (by light or by dithiothreitol) proceeds on each subunit by reduction of a disulfide bridge located at the amino terminus of the enzyme between Cys10 and Cys15.
The far-field modified uncorrelated single-scattering approximation in light scattering by a small volume element (2007)

Consider light scattering by a small volume element filled with randomly positioned particles, the far-field modified uncorrelated single-scattering approximation (MUSSA) leads to the incoherent summation of the phase matrices of particles in the volume. The validity of the MUSSA is revisited in this paper to include the variation of the particles' positions. Analytical results show that the MUSSA does not require the distance between any pair of particles in the volume to be larger than what is required in the single-scattering approximation (SSA). Instead, it requires the dimension of the volume to be large compared to the incident wavelength. The new results also make the requirements of MUSSA easier to be met. We also analyze energy conservation for the MUSSA.
Differential expression of phenylalanine ammonia-lyase and chalcone synthase during soybean nodule development. (1991)

We have used conserved and nonconserved regions of cDNA clones for phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) isolated from a soybean-nodule cDNA library to monitor the expression of members of the two gene families during the early stages of the soybean-Bradyrhizobium japonicum symbiosis. Our results demonstrate that subsets of the PAL and CHS gene families are specifically induced in soybean roots after infection with B. japonicum. Furthermore, by analyzing a supernodulating mutant line of soybean that differs from the wild-type parent in the number of successful infections, we show that the induction of PAL and CHS is related to postinfection events. Nodulated roots formed by a Nod+ Fix- strain of B. japonicum, resembling a pathogenic association, display induction of another distinct set of PAL and CHS genes. Our results suggest that the symbiosis-specific PAL and CHS genes in soybean are not induced by stress or pathogen interaction.
Small-residue packing motifs modulate the structure and function of a minimal de novo membrane protein (2020)

Alpha-helical integral membrane proteins contain conserved sequence motifs that are known to be important in helix packing. These motifs are a promising starting point for the construction of artificial proteins, but their potential has not yet been fully explored. Here, we study the impact of introducing a common natural helix packing motif to the transmembrane domain of a genetically-encoded and structurally dynamic de novo membrane protein. The resulting construct is an artificial four-helix bundle with lipophilic regions that are defined only by the amino acids L, G, S, A and W. This minimal proto-protein could be recombinantly expressed by diverse prokaryotic and eukaryotic hosts and was found to co-sediment with cellular membranes. The protein could be extracted and purified in surfactant micelles and was monodisperse and stable in vitro, with sufficient structural definition to support the rapid binding of a heme cofactor. The reduction in conformational diversity imposed by this design also enhances the nascent peroxidase activity of the protein-heme complex. Unexpectedly, strains of Escherichia coli expressing this artificial protein specifically accumulated zinc protoporphyrin IX, a rare cofactor that is not used by natural metalloenzymes. Our results demonstrate that simple sequence motifs can rigidify elementary membrane proteins, and that orthogonal artificial membrane proteins can influence the cofactor repertoire of a living cell. These findings have implications for rational protein design and synthetic biology.
Killing of Periodontopathogenic Bacteria by Photodynamic Therapy (2004)

The data collected to date suggest that photodynamic therapy with chlorin e6 and BLC 1010 is advantageous for suppressing periodontopathogenic bacteria.