gebhart/patrepeval huggingface dataset:
Construction of pPIC9 Recombinant Vector Containing Human Stem Cell Factor (2013)

rhSCF/pPIC9 vector can be transformed into the Picha pastoris yeast as a eukaryotic host in order to produce human SCF at industrial scale.
Real-time displacement measurement system using phase-shifted optical pulse interferometry: Application to a seismic observation system (2016)

Abstract We developed a method of detecting incident light levels on the oscillator surfaces and light pulses that include two interfering pulses with a phase shift of π/2 (phase-shifted optical pulse interferometry). This system enables the measurement of displacements greatly exceeding the half wavelength of the laser. Moreover, it allows measurements at multiple locations with a single optical fiber for using optical pulses. In this study, we conducted an interference experiment using 30 ns optical pulses and transmitted them at 1 µs intervals. We confirmed that the above two measurements are possible. Furthermore, from the data of the oscillator used for verification, we showed that measurements on the order of nanometers are possible. Since this method does not require a power supply to the oscillator, its widespread applications in physical exploration can be expected.
Faculty Opinions recommendation of Head rotational acceleration characteristics influence behavioral and diffusion tensor imaging outcomes following concussion. (2017)

A majority of traumatic brain injuries (TBI) in motor vehicle crashes and sporting environments are mild and caused by high-rate acceleration of the head. For injuries caused by rotational acceleration, both magnitude and duration of the acceleration pulse were shown to influence injury outcomes. This study incorporated a unique rodent model of rotational acceleration-induced mild TBI (mTBI) to quantify independent effects of magnitude and duration on behavioral and neuroimaging outcomes. Ninety-two Sprague-Dawley rats were exposed to head rotational acceleration at peak magnitudes of 214 or 350 krad/s 2 and acceleration pulse durations of 1.6 or 3.4 ms in a full factorial design. Rats underwent a series of behavioral tests including the Composite Neuroscore (CN), Elevated Plus Maze (EPM), and Morris Water Maze (MWM). Ex vivo diffusion tensor imaging (DTI) of the fixed brains was conducted to assess the effects of rotational injury on brain microstructure as revealed by the parameter fractional anisotropy (FA). While the injury did not cause significant locomotor or cognitive deficits measured with the CN and MWM, respectively, a main effect of duration was consistently observed for the EPM. Increased duration caused significantly greater activity and exploratory behaviors measured as open arm time and number of arm changes. DTI demonstrated significant effects of both magnitude and duration, with the FA of the amygdala related to both the magnitude and duration. Increased duration also caused FA changes at the interface of gray and white matter. Collectively, the findings demonstrate that the consequences of rotational acceleration mTBI were more closely associated with duration of the rotational acceleration impulse, which is often neglected as an independent factor, and highlight the need for animal models of TBI with strong biomechanical foundations to associate behavioral outcomes with brain microstructure.
Oral Delivery of Group A Streptococcal Cell Walls Augments Circulating TGF-β and Suppresses Streptococcal Cell Wall Arthritis (1998)

Abstract Oral administration of autoantigens can influence the outcome of experimental autoimmune diseases, yet little is known about nonself Ag-induced tolerance. In this study, we administered group A streptococcal cell wall (SCW) peptidoglycan-polysaccharide complexes orally and monitored the impact on SCW-induced erosive polyarthritis. Oral administration of low dose SCW (3 μg/day), initiated 7 days before an arthritogenic dose of systemic SCW, virtually eliminated the joint swelling and destruction typically observed during both the acute and chronic phases of the arthritis. High (300 μg), but not intermediate (30 μg), dose regimens also profoundly inhibited the disease. Most previous studies have demonstrated that prior feeding is required for efficacy, yet oral feeding of low dose SCW suppressed the evolution of arthritis even when administration was begun 10–15 days after induction of the arthritis. While the synovial inflammatory cell infiltration and expression of proinflammatory cytokines were markedly suppressed, no local enhancement of the regulatory cytokines IL-4, IL-10, and TGF-β was detected. Oral administration of low dose SCW, however, up-regulated circulating levels of TGF-β, concomitant with decreased circulating TNF-α and suppression of chronic arthritis. Moreover, IL-10 was increased in tolerized spleen lymphocytes, and unexpectedly, this SCW-specific IL-10 production was TGF-β dependent. These data support a pivotal role for TGF-β, although not necessarily in the joint, in the regulation of specific immune tolerance responsible for suppressed synovial inflammation and matrix destruction. The distant induction and up-regulation of regulatory cytokines and/or cells may contribute to the inhibition of the immune response through blunted infiltration of inflammatory cells to the joint.
Channeling of terawatt laser pulses by use of hollow waveguides (1995)

Subpicosecond laser pulses at power levels in excess of 1 TW were channeled through hollow microcapillary tubes by use of a combination of grazing-incidence dielectric and plasma-wall reflection mechanisms. Maximum input and output intensities were 10(17) and 10(16) W/cm(2) through 50-microm radius by 3-cm-long glass microcapillary tubes with as few as two waveguide modes being excited. 133-microm radius tubes as long as 13 cm resulted in successful channeling with an extinction coefficient of 0.2 cm(-1) and a plasma-wall reflectivity of 80%.
Critical pollution value of heavy metals contained in river sediments. (1986)

There are various means of determining the heavy metal critical pollution point of river sediments. This study presents a method for determining a critical point of pollution by using the quantities :T=_??__??_σjj' log10(a+byj) log10(a+byj')where, the suffixes j and j' correspond to the j-th and j'-th metals, respectively, the n×n matrix [σjj'] is the inverse of the population variance-covariance matrix of n heavy metals in upstream rivers, and a and b are the arbitrary constants. yj denotes the average of m independent measurements of the concentration of the j-th metal of the typical downstream river. Values of log10 (a+byj) are regarded as being distributed as N (μ, ∑), and hence T follows a noncentral chi-square distribution. Using PEARSON'S approximation for a central chi-square distribution, a critical pollution point is obtained. Application to first class rivers in Japan showed a qualitative comparison of the intensity of pollution.
Cairo University SPARC V2 (CUSPARC V2) processor (2016)

The Cairo University SPARC (CUSPARC) processor is an IP embedded processor based on SPARC V8 ISA standard. It is fully developed at Cairo University and is the first Egyptian processor. CUSPARC was implemented on several FPGA and ASIC platforms. Its first ASIC version (CUSPARC V1) was implemented on Si in 2010 using IBM 0.13μm CMOS 8RF-DM process. This paper reports the design, fabrication and testing of an enhanced version of CUSPARC, labelled CUSPARC V2. CUSPARC V2 is augmented with an ADPLL, a hardware multiplier, and four JTAG scan chains.
Electroanalysis and Coulometric Analysis (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectroanalysis and Coulometric AnalysisDonald D. DeFordCite this: Anal. Chem. 1960, 32, 5, 31–37Publication Date (Print):April 1, 1960Publication History Published online12 July 2002Published inissue 1 April 1960https://pubs.acs.org/doi/10.1021/ac60161a604https://doi.org/10.1021/ac60161a604research-articleACS PublicationsRequest reuse permissionsArticle Views371Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Histocompatibility antigens and genetic control of the immune response in guinea pigs IV. Specific inhibition of lymphocyte proliferation by auto-anti-idiotypic antibodies. (1977)

The in vitro T-cell proliferation induced by penicilloylated bovine IgG (BPO-BGG) in sensitized strain 2 guinea pigs could be specifically blocked by the serum of guinea pig (*305) which had been repeatedly immunized with BPO-BGG over a period of 9 mo. The antibodies which appeared in the serum of this animal (*305) were functionally similar to the strain 2 anti-idiotypic antibodies (a strain 2 BPO-BGG) raised by immunizing strain 2 guinea pigs with immuno-adsorbent column-purified BPO-BGG. Animal *305 had no detectable antibodies to BPO-BGG and failed to give a delayed hypersensitivity skin response when challenged with BPO-BGG.
Integrated Hydraulic Model and Genetic Algorithm Optimization for Informed Analysis of a Real Water System (2008)

A hydraulic network model was constructed for the City of Sidney, Ohio water distribution system. It consists of 104 miles of water mains, some of which are well over a hundred year old and serve the original part of the system. The City intends to use the model for available fire flow analysis and a variety of many other applications. It is essential that the hydraulic model be able to accurately simulate the real system conditions. Model calibration is the critical step to achieve this goal. To calibrate the model, Sidney's Public Utility Department collected the pressure data at twenty locations, along with the boundary conditions of the observed tank levels and pump operating status. The calibration proceeds by applying a well-integrated hydraulic simulation and genetic algorithm optimization modeling platform in two phases including the systemic demand calibration to establish an extended period flow balance model and hydraulic grade calibration under multiple loading conditions. The model calibration performed for the Sidney water distribution model improves the confidence in the modeling results. It assists the engineers to better understand the insight into the system hydraulics and enhances modeling quality for evaluating the available fire flow and system improvement design. This paper illustrates the critical steps of the calibration process and the applications of the calibrated model for the City of Sidney. This paper was presented at the 8th Annual Water Distribution Systems Analysis Symposium which was held with the generous support of Awwa Research Foundation (AwwaRF).
An Architecture for Policy-Based Home Automation System &#40;PBHAS&#41; (2010)

In order to make the smart home more autonomous, responsive to needs of inhabitants and allows energy saving; we propose architecture for self-configuring of household appliances. This architecture will be used to develop a home automation system called PBHAS (Policy-Based Home Automation System). In this architecture, we adopt the policy-based management approach for the home automation. Therefore, we propose a PDP-PRF (Policy Decision Point usage for profile) for the management of the home, a LPDP-PRF (Local Policy Decision Point usage for profile) for the management of each room, a PEP-PRF (Policy Enforcement Point usage for profile) for the management of each household appliance, a database called PB-PRF (Policy Database usage for profile) to store needed information to manage the home and, finally, COPS-PRF (Common Open Policy Service usage for profile), to ensure communication between the PDP-PRF and LPDP-PRFs
High Temperature Stress and Pollen Viability of Maize1 (1980)

High temperatures during maize ( Zea mays L.) pollinatiou are known to result in poor kernel set, but little is known of the direct effects of temperature on pollen germination. The purpose of this research was to determine how in vitru pollen germination of different maize genotypes is affected by high temperature stress during anthesis. Tassels from field‐grown plants were excised at beginning anthesis, placed in water and transferred to growth chambers maintained at daytime temperatures of 27, 32, and 38 C. Nighttime temperatures were maintained 6 C cooler. In vitro germination was measured after 24 and 48 hours in the growth chamber as well as on pollen collected directly in the field. Genotypes differed in their response to temperature. In some genotypes pollen germination steadily decreased as temperature increased. Others either germinated equally well at 27 and 32 C or germinated better at 32 than at 27 C. All genotypes had a lower germination at 38 C than at 32 or 27 C, and several genotypes exhibited no germination after 48 hours at 38 C. After 24 hours in the 38 C chamber, six inbreds widely used in the 1970's germinated significantly better as a group than inbreds widely used in the 1950's and 1930's. Growth environment affected the absolute in vitro germination percentage, but in general genotypes retained similar relative responses to increasing temperature. Results from this study suggest that prolonged exposure to temperatures above 32 C can reduce pollen germination of many genotypes to levels near zero.
Fertilization success of an arctic sea urchin species, &lt;i&gt;Strongylocentrotus&lt;/i&gt; &lt;i&gt;droebachiensis&lt;/i&gt; (O. F. Müller, 1776) under CO&lt;sub&gt;2&lt;/sub&gt;-induced ocean acidification (2013)

Abstract. Sea urchins as broadcasting spawners, release their gametes into open water for fertilization, thus being particularly vulnerable to ocean acidification. In this study, we assessed the effects of different pH scenarios on fertilization success of Strongylocentrotus droebachiensis, collected at Spitsbergen, Arctic. We achieved acidification by bubbling CO2 into filtered seawater using partial pressures (pCO2) of 180, 380, 980, 1400 and 3000 μatm}. Untreated filtered seawater was used as control. We recorded fertilization rates and diagnosed morphological aberrations after post-fertilization periods of 1 h and 3 h under different exposure conditions in experiments with and without pre-incubation of the eggs prior to fertilization. In parallel, we conducted measurements of intracellular pH changes using BCECF/AM in unfertilized eggs exposed to a range of acidified seawater. We observed increasing rates of polyspermy in relation to higher seawater pCO2, which might be due to failures in the formation of the fertilization envelope. In addition, our experiments showed anomalies in fertilized eggs: incomplete lifting-off of the fertilization envelope and blebs of the hyaline layer. Other drastic malformations consisted of constriction, extrusion, vacuolization or degeneration (observed as a gradient from the cortex to the central region of the cell) of the egg cytoplasm, and irregular cell divisions until 2- to 4-cell stages. The intracellular pH (pHi) decreased significantly from 1400 μatm on. All results indicate a decreasing fertilization success at CO2 concentrations from 1400 μatm upwards. Exposure time to low pH might be a threatening factor for the cellular buffer capacity, viability, and development after fertilization.
Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: a case study with methylcellulose (2022)

Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies. Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in <i>in vivo</i> therapies. In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS. MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS. This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner. These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.
Role of Platelets in Chronic Kidney Disease (2021)

Platelet-dependent mechanisms for excessive clotting and bleeding in CKD remain undefined. Moreover, platelets' contribution to inflammation, and specifically to CKD, are equally elusive. To date, descriptions of changes in the functional properties of circulating platelets during CKD have provided confusing interpretations. Experimental approaches that can advance our understanding of platelet dysfunction in CKD are needed, and studies that provide mechanistic insights into the dynamic relationships between thrombosis, bleeding, and inflammation associated with CKD will be essential to improve clinical management and outcomes for this vulnerable population. This article summarizes existing literature characterizing platelets in CKD and identifies areas that need further investigation.
Chromosome Replication and Segregation in Bacteria (2012)

In dividing cells, chromosome duplication once per generation must be coordinated with faithful segregation of newly replicated chromosomes and with cell growth and division. Many of the mechanistic details of bacterial replication elongation are well established. However, an understanding of the complexities of how replication initiation is controlled and coordinated with other cellular processes is emerging only slowly. In contrast to eukaryotes, in which replication and segregation are separate in time, the segregation of most newly replicated bacterial genetic loci occurs sequentially soon after replication. We compare the strategies used by chromosomes and plasmids to ensure their accurate duplication and segregation and discuss how these processes are coordinated spatially and temporally with growth and cell division. We also describe what is known about the three conserved families of ATP-binding proteins that contribute to chromosome segregation and discuss their inter-relationships in a range of disparate bacteria.
This Month in Investigative Urology (2007)

No AccessJournal of UrologyThis month in investigative urology1 Jan 2008This Month in Investigative Urology Anthony Atala Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2007.10.003AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "This Month in Investigative Urology." The Journal of Urology, 179(1), pp. 7–8 © 2008 by American Urological AssociationFiguresReferencesRelatedDetails Volume 179Issue 1January 2008Page: 7-8 Advertisement Copyright & Permissions© 2008 by American Urological AssociationMetricsAuthor Information Anthony Atala More articles by this author Expand All Advertisement PDF downloadLoading ...
Study on transition charging method of lithium-ion battery based on the multiple model adaptive control (2011)

For Traditional constant current/voltage charging method could cause temperature rise phenomenon with large current charging in later period, so this paper use transition charging method between constant current period and constant voltage period to adapt to the chemical reaction rate in the battery. And for the poor adaptability of general PI controller, this paper use multiple model adaptive control methods to improve the adaptability of controller and efficiency. It also ensure the accuracy and battery performance.
Airline fleet maintenance: Trade-off analysis of alternate aircraft maintenance approaches (2011)

Airline maintenance is a significant contributor to the overall operating costs of airlines, making up 11% of the total operating costs. In fact, the cost of airline maintenance has steadily increased in recent years. An improvement in the approach airlines take to maintain aircraft is needed to reverse this trend. Currently, aircraft operators use a preventative maintenance approach, where parts are replaced on a schedule. The purpose of this project is to examine the effectiveness of utilizing a condition-based approach, which considers the actual physical condition of the parts onboard the aircraft to dictate the time at which they are replaced. This additional information would allow parts to be replaced when they near the end of their actual usable life, thus reducing unnecessary replacements. These two approaches of maintenance were decomposed into three alternatives. The first alternative uses a preventative approach whereas the other two alternatives are condition-based. The preventative alternative involves the transmission of maintenance data to maintenance personnel when the aircraft is out of service. The second alternative, Condition-Based with Flight Line Transfer, involves the transmission of part condition information in between flights. The third alternative, Condition-Based with Airborne Transfer, provides a near realtime condition monitoring system during flight operations. The three design alternatives were compared in a discrete event simulation, using ARENA <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">®</sup> , to determine the overall benefits of each maintenance approach. They were then evaluated using a utility function to determine their overall value to the system's stakeholders. Based on the results from the simulation, the Flight Line alternative ranked first out of the three considered alternatives. However, the aircraft operators could realize the most benefit by applying the condition-based process to parts with a high infant mortality failure pattern with low mean times between failure given that the other costs (shipping, storage, ordering, etc.) remain the same. The preventative alternative, though it had a very low implementation cost, resulted in more total replaced parts and a lower percentage of parts replaced with notification. While the Airborne alternative provided nearly perfect notification for part replacements, the high implementation and operating costs greatly offset its overall value to the stakeholders.