gebhart/patrepeval huggingface dataset:
High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans (2006)

Telomerase is essential to maintain telomere length in most eukaryotes. Other functions for telomerase have been proposed but molecular mechanisms remain unclear. We studied Caenorhabditis elegans with a mutation in the trt-1 telomerase reverse transcriptase gene. Mutant animals showed a progressive decrease in brood size and typically failed to reproduce after five generations. Using PCR analysis to measure the length of individual telomere repeat tracks on the left arm of chromosome V we observed that trt-1 mutants lost approximately 125bp of telomeric DNA per generation. Chromosome fusions involving complex recombination reactions were observed in late generations. Strikingly, trt-1 mutant animals displayed a high frequency of telomeres with many fewer repeats than average. Such outlying short telomeres were not observed in mrt-2 mutants displaying progressive telomere loss very similar to trt-1 mutants. We speculate that, apart from maintaining the average telomere length, telomerase is required to prevent or repair sporadic telomere truncations that are unrelated to the typical 'end-replication' problems.
A simple and rapid amplification procedure for cDNA cloned in dephosphorylated plasmid (1993)

The preparation of cDNA libraries usually involves multiple steps, such as isolation of poly A+ mRNA, synthesis of cDNA, cloning of cDNA into plasmid, and amplification of cloned plasmid. The last step, the amplification of insert DNA fragment after cloned in dephosphorylated plasmid vector, is done in either of two ways. The recombinant DNA can be extracted after amplification in host cells, or the amplification of insert DNA can be done by the PCR using primers complementary to both ends of the insertion site of the vector. However, the ligation of dephosphorylated nicks or nick translation beyond the primer sites is essential in the latter case prior to the PCR (see step 1 in Figure 1 (B)). Taq DNA polymerase, by virtue of its 5' to 3' exonuclease activity (Ref 1), may be utilized for nick translation instead of E. coli DNA polymerase 1. This would simplify the amplification of insert cDNA by doing nick translation and PCR steps successively, controlling each reaction condition. We examined the conditions for nick translation by Taq DNA polymerase. The reaction mixture, 20 /tl containing 25 yCi ^PdNTPs (600 Ci/mmol), 0.5 ng RF-M13mpl8 DNA, 0.02 ng pancreatic DNase I, and 2.5 units of Taq DNA polymerase was incubated at 37, 50 and 65°C and the incorporation of 32PdNTPs into acid-insoluble fraction was measured. Radioactivities incorporated were 167 k, 323 k, and 286 k DPM, respectively, compared with 82 k DPM by a standard nick translation reaction at 12°C using DNA polymerase I and DNase I. These results indicated that the nick translation reaction by Taq DNA polymerase worked normally at all temperatures examined, although the incorporation was reduced at 65°C. Then we amplified insert DNA by nick translation reaction
List decoding reed-solomon, algebraic-geometric, and gabidulin subcodes up to the singleton bound (2013)

We consider Reed-Solomon (RS) codes whose evaluation points belong to a subfield, and give a linear-algebraic list decoding algorithm that can correct a fraction of errors approaching the code distance, while pinning down the candidate messages to a well-structured affine space of dimension a constant factor smaller than the code dimension. By pre-coding the message polynomials into a subspace-evasive set, we get a Monte Carlo construction of a subcode of Reed-Solomon codes that can be list decoded from a fraction (1-R-ε) of errors in polynomial time (for any fixed ε > 0) with a list size of O(1/ε). Our methods extend to algebraic-geometric (AG) codes, leading to a similar claim over constant-sized alphabets. This matches parameters of recent results based on folded variants of RS and AG codes. but our construction here gives subcodes of Reed-Solomon and AG codes themselves (albeit with restrictions on the evaluation points).
Digital adaptive flight controller development (1973)

Digital fly-by-wire flight control systems are currently of interest because simple mechanical linkages are no longer able to cope with the many control problems associated with high performance aircraft. In view of this, a digital adaptive flight control algorithm has been developed by combining a weighted least squares estimator with optimal linear regulator control logic designed to minimize a quadratic model following performance index. Results for a typical fighter aircraft's trajectory over six distinct flight conditions are very promising.
Konservative Frakturbehandlung bei Hund und Katze (2023)

In bestimmten Situationen stellt die konservative Frakturbehandlung eine gute Option zur Knochenheilung nach einer Fraktur dar. Welche Kriterien bei der Therapieentscheidung zu beachten sind und welche Methoden der konservativen Behandlung zur Auswahl stehen, erfahren Sie im vorliegenden Beitrag.
Percutaneous Transtracheal Ventilation: Resuscitation Bags Do Not Provide Adequate Ventilation (2006)

Resuscitation bags should not be used to ventilate adult patients through a 14-gauge, transtracheal catheter. Jet ventilation is needed when percutaneous transtracheal ventilation is attempted. If jet ventilation is attempted using oxygen supply tubing, it must be connected to an unregulated oxygen source of at least 50 psi.
Supplementary Data from Trilaciclib Prior to Chemotherapy in Patients with Metastatic Triple-Negative Breast Cancer: Final Efficacy and Subgroup Analysis from a Randomized Phase II Study (2023)

Supplementary Data from Trilaciclib Prior to Chemotherapy in Patients with Metastatic Triple-Negative Breast Cancer: Final Efficacy and Subgroup Analysis from a Randomized Phase II Study
Notes del col·loqui internacional : les inondations d'Octobre 1940 en Catalogne. 50 ans passés (1991)

Du 18 au 20 Octobre 1990 a eu lieu a Vernet-les-Bains (France) un coloque sur les inondations d'Octobre de 1940 en Catalogne. Son but etait de reflechir sur les circonstances qui ont produit la catastrophe, mais aussi d'en tirer des enseignements qui permettent la prevention. Des activites paralleles (des expositions graphiques, des audiovisuels, des expositions de documents et una sortie de travail) ont montre que l'etude des inondations reste un sujet ne pouvant pas etre oublie surtout alors qu'il existe une tendance croissante a negliger ce risque naturel.
Las órdenes e instrucciones particulares del Ministerio Fiscal: una propuesta de reforma a la luz del sistema francés (2012)

Estas lineas pretenden ser una consideracion doctrinal acerca de un tema controvertido y escoyo a una autentica autonomia del Ministerio Publico, comparando para ello el sistema actual de ordenes e instrucciones vigente en Espana que se regula en el EOMF segun la redaccion dada por la Ley 24/2007 con el sistema contemplado por el derecho frances y hacer una breve mencion a la situacion en el espacio judicial europeo, con la finalidad que de tal comparacion se puedan extraer algunas conclusiones que en caso de considerarse adecuadas puedan ser extrapolables a nuestro derecho nacional, dejando siempre constancia con caracter previo de las diferencias existentes entre uno y otro sistema.
Early Alterations in Neutrophil Activation Are Associated with Outcome in Acute Lung Injury (2003)

Persistent elevations of proinflammatory cytokines in the lungs are associated with increased mortality from acute lung injury (ALI), suggesting that the degree of pulmonary inflammation is an important determinant of clinical course in ALI. The transcriptional regulatory factor nuclear factor-kappaB (NF-kappaB) is involved in modulating the expression of many cytokines and other proinflammatory mediators implicated in the development and progression of ALI. Because neutrophils appear to play a major role in the development of ALI, we examined the relationships between clinical outcome and activation of NF-kappaB in peripheral neutrophils from patients (n = 30) with sepsis-induced ALI. We found that nuclear translocation of NF-kappaB in this setting was dependent on the activation of p38 and Akt kinases. Diminished activation of NF-kappaB or Akt, but not p38, in the early postintubation period was associated with less time on the ventilator and improved survival in critically ill patients with ALI. These results suggest that early alterations in neutrophil activation patterns, particularly involving the ability to accumulate NF-kappaB to the nucleus after relevant stimuli, contribute to subsequent clinical course in ALI.
TiO2 Nanoparticle Arrays Prepared Using a Nanosphere Lithography Technique (2002)

A nanosphere lithography technique has been used to synthesize periodic nanoparticle arrays of TiO2 on glass substrates. Both monolayer and bilayer evaporation masks were generated from hexagonally close-packed polystyrene nanospheres, each one producing a different array of TiO2 nanoparticles. Atomic force microscopy (AFM) showed that the masks typically consisted of ordered 10−100 μm2 domains. X-ray photoelectron spectroscopy confirmed that the surface composition of the particles corresponded to TiO2 with minor amounts of a Ti3+ species, presumably associated with edges, corners, and oxygen vacancy defects. Analysis of the AFM images indicated that the nanoparticles were circular in shape with array dimensions approximately consistent with simple geometric considerations for the 420 nm diameter polystyrene nanospheres used as masks in this work. The monolayer and bilayer masks yielded TiO2 particle diameters of 169 ± 12 nm and 140 ± 13 nm, respectively. The absorption edge of the nanoparticle arrays are blue-shifted from single-crystal rutile.
A Data Driven Empirical Iterative Algorithm for GSR Signal Pre-Processing (2018)

In this paper, we introduce a data driven iterative low pass filtering technique, the Empirical Iterative Algorithm (EIA) for Galvanic Skin Response (GSR) signal preprocessing. This algorithm is inspired on Empirical Mode Decomposition (EMD), with performance enhancements provided by applying Midpoint-based Empirical Decomposition (MED), and removing the sifting process in order to make it computational inexpensive while maintaining effectiveness towards removal of high frequency artefacts. Based on GSR signals recorded at the wrist we present an algorithm benchmark, with results from EIA being compared with a smoothing technique based on moving average filter - commonly used to pre-process GSR signals. The comparison is established on data from 20 subjects, collected while performing 33 different randomized activities with right hand, left hand and both hands, respectively. In average, the proposed algorithm enhances the signal quality by 51%, while the traditional moving average filter reaches 16% enhancement. Also, it performs 136 times faster than the EMD in terms of average computational time. As a show case, using the GSR signal from one subject, we inspect the impact of applying our algorithm on GSR features with psychophysiological relevance. Comparison with no preprocessing and moving average filtering shows the ability of our algorithm to retain relevant low frequency information.
Port Authority of NY & NJ: Experiences with Aeronautical Asphalt Pavements (2001)

The experience of The Port Authority of NY & NJ, with the mixes presented, has been a reduction of plastic deformation. Of course, this is predicated on the assumption that these mixes are not placed on existing asphalt pavements that have been softened with jet fuel and/or hydraulic fluids. These mixes can be made stiffer by adopting the SHRP Level I mix design changes recommended. In addition, polymer modified asphalt with a PG of 76-22 and 82-22 adds to the design life of the pavement. To realize the full benefit of these mix designs a genuine QC/ QA plan is required for batching, loading and placing these mixes. The mixes are more susceptible to segregation and can produce an open surface texture, problems which can be alleviated by following good asphalt batching and placing procedures. Some agencies have specified the use of Re-Mix Pavers and/or Materials Transfer Vehicles to minimize the segregation problem.
Semi-parametric models for visual odometry (2012)

This paper introduces a novel framework for estimating the motion of a robotic car from image information, a scenario widely known as visual odometry. Most current monocular visual odometry algorithms rely on a calibrated camera model and recover relative rotation and translation by tracking image features and applying geometrical constraints. This approach has some drawbacks: translation is recovered up to a scale, it requires camera calibration which can be tricky under certain conditions, and uncertainty estimates are not directly obtained. We propose an alternative approach that involves the use of semi-parametric statistical models as means to recover scale, infer camera parameters and provide uncertainty estimates given a training dataset. As opposed to conventional non-parametric machine learning procedures, where standard models for egomotion would be neglected, we present a novel framework in which the existing parametric models and powerful non-parametric Bayesian learning procedures are combined. We devise a multiple output Gaussian Process (GP) procedure, named Coupled GP, that uses a parametric model as the mean function and a non-stationary covariance function to map image features directly into vehicle motion. Additionally, this procedure is also able to infer joint uncertainty estimates (full covariance matrices) for rotation and translation. Experiments performed using data collected from a single camera under challenging conditions show that this technique outperforms traditional methods in trajectories of several kilometers.
Flow cytometry and single nucleus sorting for Cre‐based analysis of changes in transcriptional states (2016)

The organs of eukaryotic organisms comprise complex interspersions of cell types, whose different molecular activities, and corresponding cellular states, cooperate during development to produce the final, functional organ. Dysfunction of organs in disease, particularly oncogenesis, initiates with changes of state of a minor subset of cells. It therefore is hard to detect early molecular indicators of disease within an overwhelming background of normal cells. Flow cytometry and sorting provides a convenient way to purify minority subpopulations, if a specific fluorophore can be unambiguously and exclusively associated with this subpopulation. We have generated a number of transgenic mouse lines expressing a nuclear-localized version of the Green Fluorescent Protein (GFP), within which the production of a chimeric histone 2B-GFP protein occurs under the control of a constitutively-active, actin-derived promoter, separated by a Floxed-STOP sequence. In the presence of Cre recombinase, within F1 progeny of these mouse lines, excision of the STOP sequence activates transcription which results in the emergence of cells containing green fluorescent nuclei. We describe the characterization of these lines using a combination of microscopic imaging, flow cytometry and sorting, and Reverse-Transcription polymerase chain reaction of transcripts within single sorted nuclei isolated from tissue homogenates. These lines should be particularly useful for analysis of transcriptional changes in oncogenesis. © 2016 International Society for Advancement of Cytometry.
Subzero nonfreezing storage of rat hepatocytes using modified University of Wisconsin solution (mUW) and 1,4-butanediol. I-effects on cellular metabolites during cold storage (2009)

Various cryopreservation techniques have been investigated to extend the storage of isolated hepatocytes; however, most have a reduced viability after rewarming due to ice crystal formation. Subzero nonfreezing conditions could theoretically reduce organ metabolism without damage due to ice crystal formation. In the present work we evaluated the viability and metabolic parameters of isolated rat hepatocytes preserved in subzero nonfreezing condition. Cell suspensions were maintained in modified University of Wisconsin (mUW) solution using 8% - ,4-butanediol as cryoprotectant, up to 120 h at -4 masculineC. The time course evolution of hepatocytes viability were measured by LDH release and propidium iodide assay. The cellular concentrations of glutathione, ATP, glycogen and the lactate production during cold storage were also determined. Finally, results were compared with conventional hypothermic storage at 0 masculineC in mUW solution without cryoprotectant. After 5 days of subzero storage, we found an improvement in the ability of rat hepatocytes to maintain the metabolic resources in comparison with the cold preserved group.
Case report: Neonatal case of intrauterine gastrointestinal bleeding with suspected cow's milk allergy or neonatal transient eosinophilic colitis (2023)

The patient was a female newborn. Ultrasonography performed at 35 weeks and 3 days of gestation revealed honeycomb-like dilatation and peri-intestinal strong echo patterns in the gastrointestinal tract. Nonreassuring fetal status was also diagnosed, leading to an emergency Cesarean section. The baby's birth weight was 2,127 g, whereas the Apgar 1 min and 5 min scores were 8 and 9, respectively. The amniotic fluid showed fecal and hematogenous turbidity. After delivery, there was hematogenous intragastric residue and defecation. Thereafter, the bloody intragastric residue and fecal discharge improved. Aggregations of eosinophils in the stool were observed, and gastrointestinal allergy was suspected. Enteral feeding with the hydrolyzed protein formula was initiated and symptoms did not recur. The allergen-specific lymphocyte stimulation test was positive for lactoferrin, and the patient was suspected with neonatal cow's milk allergy or neonatal transient eosinophilic colitis. After her condition stabilized, an oral challenge test was performed using breast milk without dairy products, and the test was negative. Gastrointestinal allergy is rare even <i>in utero</i>, and when gastrointestinal bleeding is suspected <i>in utero</i>, hemorrhagic or surgical gastrointestinal diseases should be ruled out first; however, the possibility of gastrointestinal allergy should also be kept in mind.
Development of Tire Force Model for Vehicle Dynamics Analysis Including the Effect of Tire Surface Temperature (2005)

The results of vehicle performance tests often vary depending on differences in the road surface temperature. It is believed that these changes can partially be attributed to the effect of tire surface temperature. The aim of this study is to develop a tire force model considering the dependence of tire surface temperature. The newly developed tire model is composed of two functions, the thermodynamic function that allows us to consider changes in the tire surface temperature and the force function that allows for the effects of tire surface temperature. The parameters of this model are identified using the measured data obtained with an indoor test facility. To prove the validity of the developed model, the surface temperature and the tire force values are predicted using this model. The simulation results agreed rather well with the experimental results.
On the Breakdown Voltage Temperature Dependence of High-Voltage Power Diode Passivated with Diamond-Like Carbon (2021)

Diamond-Like Carbon (DLC) is well established material for the passivation of high voltage negative beveled power diode. In our previous works, the conduction mechanism of the DLC has been carefully described through the characterization and the physical modeling of Metal-Insulator-Semiconductor (MIS) structures. In addition, the effects on the breakdown voltage and leakage current have been clarified comparing the available experiments with numerical simulations. However, the role played by the DLC on the breakdown voltage temperature dependence is still lacking. In this work, it has been investigated assuming a release of the trapped charges with increasing temperatures.
Factor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment (2003)

Activated platelets release their granule content in a concentrated fashion at sites of injury. We examined whether ectopically expressed factor VIII in developing megakaryocytes would be stored in alpha-granules and whether its release from circulating platelets would effectively ameliorate bleeding in a factor VIIInull mice model. Using the proximal glycoprotein 1b alpha promoter to drive expression of a human factor VIII cDNA construct, transgenic lines were established. One line had detectable human factor VIII that colocalizes with von Willebrand factor in platelets. These animals had platelet factor VIII levels equivalent to 3% to 9% plasma levels, although there was no concurrent plasma human factor VIII detectable. When crossed onto a factor VIIInull background, whole blood clotting time was partially corrected, equivalent to a 3% correction level. In a cuticular bleeding time study, these animals also had only a partial correction, but in an FeCl3 carotid artery, thrombosis assay correction was equivalent to a 50% to 100% level. These studies show that factor VIII can be expressed and stored in platelet alpha-granules. Our studies also suggest that platelet-released factor VIII is at least as potent as an equivalent plasma level and perhaps even more potent in an arterial thrombosis model.
Elevated levels of serum creatinine: recommendations for management and referral. (1999)

This clinical practice guideline has been endorsed by the Canadian Society of Nephrology and the College of Family Physicians of Canada. Meeting, teleconference and travel expenses of the Referral Guideline Subcommittee were covered by The Momentum Program, a collaboration between Baxter Corp. and Janssen-Ortho Inc. However, the authors are solely responsible for the editorial content of this article.
Treatment strategies for dystonia (2009)

Treatment of dystonia begins with proper diagnosis and classification, followed by an appropriate search for underlying etiology, and an assessment of the functional impairment associated with the dystonia. The therapeutic approach, which is usually limited to symptomatic therapy, must then be tailored to the individual needs of the patient.
Primary organization of nucleosomal core particles is invariable in repressed and active nuclei from animal, plant and yeast cells (1985)

A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in the following order: (5') H2B25,35-H455,65-H375,85,95/H488-H2B105,11 5-H2A118-H3135,145/H2A145 (3') (the subscripts give approximate distance in nucleotides of the main histone contacts from the 5'-end). Hence, the histone tetramer (H3,H4)2 and two dimers (H2A-H2B) are arranged on double-stranded core DNA in a symmetrical and rather autonomous way: H2A/H3-(H2A-H2B)-(H3,H4)2-(H2B-H2A)-H3/H2A. The primary organization was found to be very similar in core particles isolated from repressed nuclei of sea urchin sperm and chicken erythrocytes, from active in replication and transcription nuclei of Drosophila embryos and yeast and from somatic cells of lily. These data show that (i) the core structure is highly conserved in evolution and (ii) the overall inactivation of chromatin does not affect the arrangement of histones along DNA and thus does not seem to be regulated on this level of the core structure.
Supplementary Figure 7 from Elevation of c-FLIP in Castrate-Resistant Prostate Cancer Antagonizes Therapeutic Response to Androgen Receptor–Targeted Therapy (2023)

&lt;p&gt;PDF file, 80KB, Bar graphs illustrating the effect of SAHA, bicalutamide or their combined administration upon (A), the AR-target gene PSA or (B) the NF-κB target gene Bcl-2 in VCaP cells.&lt;/p&gt;