gebhart/patrepeval huggingface dataset:
Integrated analysis of microRNA and mRNA expression profiles in physiological myelopoiesis: role of hsa-mir-299-5p in CD34+ progenitor cells commitment (2010)

Hematopoiesis entails a series of hierarchically organized events that proceed throughout cell specification and terminates with cell differentiation. Commitment needs the transcription factors' effort, which, in concert with microRNAs, drives cell fate and responds to promiscuous patterns of gene expression by turning on lineage-specific genes and repressing alternate lineage transcripts. We obtained microRNA profiles from human CD34+ hematopoietic progenitor cells and in vitro differentiated erythroblasts, megakaryoblasts, monoblasts and myeloblast precursors that we analyzed together with their gene expression profiles. The integrated analysis of microRNA-mRNA expression levels highlighted an inverse correlation between microRNAs specifically upregulated in one single-cell progeny and their putative target genes, which resulted in downregulation. Among the upregulated lineage-enriched microRNAs, hsa-miR-299-5p emerged as having a role in controlling CD34+ progenitor fate, grown in multilineage culture conditions. Gain- and loss-of-function experiments revealed that hsa-miR-299-5p participates in the regulation of hematopoietic progenitor fate, modulating megakaryocytic-granulocytic versus erythroid-monocytic differentiation.
Effects of <i>β</i>-Sitosteryl Sulfate on the Hydration Behavior of Dipalmitoylphosphatidylcholine (2018)

We investigated the hydration behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers containing sodium β-sitosteryl sulfate (PSO<sub>4</sub>). PSO<sub>4</sub> was found to enhance hydration in the headgroup region of DPPC bilayers. Therefore, with the incorporation of PSO<sub>4</sub> into DPPC membranes, the amount of water required to reach the fully hydrated state was enhanced as indicated by the constant values of the main phase transition temperature (T<sub>m</sub>) and the bilayer repeat distance (d). For example, with the addition of 20 mol% of PSO<sub>4</sub>, the saturation point was shifted to ~70 wt% water compared to ~40 wt% for pure DPPC and 47 wt% for DPPC-cholesterol. The effectiveness of PSO<sub>4</sub> in fluidizing the membrane and enhancing its hydration state can be useful in the pharmaceutical and cosmetic industries.
Linearisation of a cascaded system of analogue optical links (1978)

We describe a linearisation technique suitable for compensation of nonlinearities in a cascaded system of analogue optical links. Experimentally, we have observed an improvement of more than 30 dB in 2nd-order distortion, reducing distortion to −53 dB in a cascaded system of four optical transmitter/receiver modules.
Tests of the radiation hardness of VLSI integrated circuits and silicon strip detectors for the SSC under neutron, proton, and gamma irradiation (1991)

As part of a program to develop a silicon strip central tracking detector system for the Superconducting Super Collider (SSC), the effects of radiation damage in silicon detectors and their associated front-end readout electronics are being studied. The authors report on the results of neutron and proton irradiations at the Los Alamos National Laboratory and gamma -ray irradiations at UC Santa Cruz. Individual components on single-sided AC-coupled silicon strip detectors and on test structures were tested. Circuits fabricated in a radiation-hard CMOS process and individual transistors fabricated using dielectric isolation bipolar technology were also studied. Bulk damage to the silicon itself is seen as the limiting factor in the lifetime of a detector system. In particular, it is the acceptor site creation in the active volume of the silicon detector that will limit the lifetime to approximately 10 yr for the innermost detectors.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
배호흡운동과 가슴우리팽창운동이 돌림근띠 손상환자의 머리위치 및 어깨자세에 미치는 영향 (2016)

PURPOSE: The aim of this study was to examine the effects of abdominal breathing and thoracic expansion exercises on craniovertebral and cranial rotation angles in patients with rotator cuff injury. METHODS: This study enrolled 19 patients with rotator cuff injury, and ten and nine of the patients were randomly placed in abdominal breathing and thoracic expansion exercise groups, respectively. After pain treatment in each group, breathing exercise was conducted thrice a week for four weeks. The patients were recorded by using a digital camera and Image J (version 1.46, National Institutes of Health, USA), an angle measurement program, was used to analyze changes in the craniovertebral angle, cranial rotation angle, and sagittal shoulder posture. RESULTS: Statistically significant differences in the craniovertebral angle were found in both the abdominal breathing and thoracic expansion exercise groups (p .05). CONCLUSION: Although abdominal breathing and thoracic expansion exercises did not effectively change sagittal shoulder posture, the exercises were effective in improving craniovertebral and cranial rotation angles. Therefore, abdominal breathing and thoracic expansion exercises are suggested as effective exercise programs for forward head posture.
Out of equilibrium magnetic behavior of a frozen ferrofluid (2005)

Présentation orale, présentée par V. Dupuis
Synthesis and kinetics research of ZnS nanoparticles prepared by sonochemical process (2009)

ZnS nanocrystallites have been successfully prepared by a sonochemical process. The reaction kinetics of the process was also investigated. The as prepared ZnS nanocrystallites were characterised by XRD and TEM. Results show that ZnS nanoparticles can be obtained by sonochemical process using ZnCl 2 and thiacetamide as raw materials. It is found that the as prepared ZnS nanoparticles are hexagonal phase with spherical or spherical-like morphologies. The grain size decreases with increasing ultrasonic irradiation power. Reaction kinetics shows that the weight content of ZnS nanoparticles increases linearly with reaction time at different temperatures. The synthesis activation energy of ZnS nanoparticles is calculated to be 27·80 kJ mol –1 .
Puupehenol, a Potent Antioxidant Antimicrobial Meroterpenoid from a Hawaiian Deep-Water Dactylospongia sp. Sponge (2015)

From the organic extract of a deep-water Hawaiian sponge Dactylospongia sp., a new potent antioxidant and antimicrobial meroterpenoid, puupehenol (1), was isolated. The structure of 1 was determined using spectroscopic techniques ((1)H and (13)C NMR, MS, IR, UV, [α]D). The known compound puupehenone (2) was also isolated and suggested as a probable artifact of the isolation procedures. Complete unambiguous (1)H and (13)C NMR data are provided for compounds 1 and 2. Bioassays performed with 1 and 2 showed them both to be very effective antioxidants and to have antimicrobial properties.
Age‐dependent loss of parvalbumin‐expressing hippocampal interneurons in mice deficient in CHL1, a mental retardation and schizophrenia susceptibility gene (2015)

In humans, deletions/mutations in the CHL1/CALL gene are associated with mental retardation and schizophrenia. Juvenile CHL1-deficient (CHL1(-/-) ) mice have been shown to display abnormally high numbers of parvalbumin-expressing (PV(+) ) hippocampal interneurons and, as adults, display behavioral traits observed in neuropsychiatric disorders. Here, we addressed the question whether inhibitory interneurons and synaptic plasticity in the CHL1(-/-) mouse are affected during brain maturation and in adulthood. We found that hippocampal, but not neocortical, PV(+) interneurons were reduced with age in CHL1(-/-) mice, from a surplus of +27% at 1 month to a deficit of -20% in adulthood compared with wild-type littermates. This loss occurred during brain maturation, correlating with microgliosis and enhanced interleukin-6 expression. In parallel with the loss of PV(+) interneurons, the inhibitory input to adult CA1 pyramidal cells was reduced and a deficit in short- and long-term potentiation developed at CA3-CA1 excitatory synapses between 2 and 9 months of age in CHL1(-/-) mice. This deficit could be abrogated by a GABAA receptor agonist. We propose that region-specific aberrant GABAergic synaptic connectivity resulting from the mutation and a subsequently enhanced synaptic elimination during brain maturation lead to microgliosis, increase in pro-inflammatory cytokine levels, loss of interneurons, and impaired synaptic plasticity. Close homolog of L1-deficient (CHL1(-/-) ) mice have abnormally high numbers of parvalbumin (PV)-expressing hippocampal interneurons in juvenile animals, but in adult animals a loss of these cells is observed. This loss correlates with an increased density of microglia (M), enhanced interleukin-6 (IL6) production and a deficit in short- and long-term potentiation at CA3-CA1 excitatory synapses. Furthermore, adult CHL1(-/-) mice display behavioral traits similar to those observed in neuropsychiatric disorders of humans.
Hydrido mercapto and bis(mercapto) derivatives of ruthenium(II) phosphine complexes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrido mercapto and bis(mercapto) derivatives of ruthenium(II) phosphine complexesPhilip G. Jessop, Chung Li Lee, Golnar Rastar, Brian R. James, Colin J. L. Lock, and Romolo FaggianiCite this: Inorg. Chem. 1992, 31, 22, 4601–4605Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/ic00048a030https://doi.org/10.1021/ic00048a030research-articleACS PublicationsRequest reuse permissionsArticle Views147Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Non-invasive optical mapping of the piglet brain in real time (1999)

We have performed non-invasive, real-time optical mapping of the piglet brain during a subcortical injection of autologous blood. The time resolution of the optical maps is 192 ms, thus allowing us to generate a real-time video of the growing subcortical hematoma. The increased absorption at the site of blood injection is accompanied by a decreased absorption at the contralateral brain side. This contralateral decrease in the optical absorption and the corresponding time traces of the cerebral hemoglobin parameters are consistent with a reduced cerebral blood flow caused by the increased intracranial pressure.
Practical and Scalable Synthetic Method for Preparation of Dolutegravir Sodium: Improvement of a Synthetic Route for Large-Scale Synthesis (2019)

A practical and scalable synthetic method to obtain dolutegravir sodium (1) was established starting from the readily accessible material maltol (2). This synthetic method includes a scalable oxidation process of maltol and palladium-catalyzed amidation for introduction of an amide moiety, leading to a practical manufacturing method in short synthetic steps. The synthetic method demonstrated herein enables multikilogram scale manufacturing of 1 of high purity.
Acute Direct Adenoviral Vector Cytotoxicity and Chronic, but Not Acute, Inflammatory Responses Correlate with Decreased Vector-Mediated Transgene Expression in the Brain (2001)

The potential utility of adenoviruses for the treatment of chronic neurological disease is controversial due to reports of vector-associated toxicity, inflammation, and transient transgene expression. To focus upon the mechanism by which transgene expression is lost, we injected increasing doses [1 x 10(6) to 1 x 10(9) infectious units (iu)] of a first-generation adenovirus vector expressing beta-galactosidase into the brains of immune-competent adult rats. Transgene expression was evaluated simultaneously with acute neuronal and glial cell cytotoxicity, and acute and chronic inflammation using immunohistochemistry, at 3 and 30 days post-vector administration. Our results show a clear threshold effect of viral dose upon the amount of transgene expression persisting by 30 days after vector administration. Below 10(8) iu, transgene expression remained stable over the 30-day period. Following infection of more than 10(8) iu, the extent of transgene expression at 30 days was inversely correlated with increasing viral dose. The severity of acute inflammation increased proportionally with increasing vector dose from 10(6) to 10(9) infectious units. In contrast, acute vector-mediated cytotoxicity and chronic inflammation were observed only above the threshold level of vector dose. Above 10(8) iu both the extent of the acute toxicity and the severity of the chronic inflammation were inversely correlated with transgene expression at 30 days. Thus, our data suggest that both an acute loss of cells through direct vector-mediated toxicity and the elicitation of chronic inflammation (but not acute inflammation) may account for the decline in transduction persistence at high vector doses.
Surface modification of PVDF hollow fiber ultrafiltration membranes for biopharmaceutical products, virus, and bacterial phage removal technology (2023)

In this study, radicals were chemically formed at the hydroxyl (-OH) sites on the surface of polyvinylidene fluoride (PVDF) hollow fiber membranes, and anionic-hydrophilic and hydrophobic materials were grafted onto the surface through -OH radical reaction to generate anions (−28, −41 mV). These membranes were then used as biomembranes (BMs) and showed excellent properties. We also studied the effects of chemical surface modification and reduction in pore size (60–130 nm) according to molecular weight. After anionic modification, the electrostatic repulsion between the pore surface and the bacteria prevents adsorption when anionic bacteria pass through the pores. Particles do not pass through the asymmetric pores due to agglomeration, resulting in separation. The hydrophilic membrane had a high initial water flux, but the flux gradually decreased due to fouling. In contrast, the hydrophobic modified membrane had a low initial water flux that eventually stabilized due to excellent fouling resistance. The protein recovery ratio (PRR) was 98.8 % for hydrophilic particles and 99.2 % for hydrophobic molecules, showing a higher PRR than the pristine membrane (96.4 %). An evaluation of removal of the gram-negative bacteria Brevundimonas diminuta (Bd) showed that no bacteria in membrane-purified solutions, confirming 100 % removal efficiencies. The developed membrane can be used in biopharmaceutical production and removal of proteins, retro viruses, and bacteria.
Ultrabroadband self-phase-stabilized pulses by difference-frequency generation (2004)

Ultrabroadband near-IR (800-1700-nm) pulses are obtained by type II difference-frequency generation (DFG) between two synchronized noncollinear optical parametric amplifiers. Self-stabilization of the carrier-envelope offset phase, as expected from the DFG process, is demonstrated experimentally.
Paxillin's LD4 motif interacts with bcl‐2 (2007)

Bcl-2 is the founding member of a family of proteins which influences cell survival in response to a variety of stimuli including those from growth factor receptors and integrins. However, how these activities are coordinated through bcl-2 requires further investigation. bcl-2 interacts with paxillin, potentially linking cell survival and cell adhesive pathways. Paxillin is an adapter protein implicated in growth factor and integrin-mediated signal transduction pathways. Previous work in this laboratory demonstrated that loss of bcl-2 affects cell adhesion and migration characteristics of renal epithelial cells, perhaps through disruption of its interaction with paxillin. Here studies were performed to determine the bcl-2 binding motif in paxillin. The amino-terminal portion of paxillin, specifically its LD4 motif, was found to associate with bcl-2. However, the amino-terminal portion of paxillin with the LD4 domain deleted did not associate with bcl-2. The corresponding LD motif in other paxillin family members, Hic-5 and leupaxin, did not associate with bcl-2. Mutations in paxillin's LD4 motif made to mimic Hic-5 and leupaxin LD4-like motifs (E(268) --> R or S(272) --> H) abolished its association with bcl-2. Incubation of embryonic kidneys with paxillin's LD4 motif disrupted ureteric bud branching and morphogenesis, while incubation with the comparable Hic-5 LD motif did not significantly affect morphogenesis. These data suggest that paxillin's association with bcl-2 plays a unique role during kidney development that other paxillin family members may not be able to fulfill.
The gentamicin antibiotics. Part II. Separation and degradation of the gentamicin C components. The purpurosamines, a new class of naturally occurring 2,6-diaminomonosaccharides (1971)

Separation of the gentamicin C components by column chromatography and subsequent mercaptolysis of their per-N-acetyl derivatives has permitted the isolation of derivatives of three new amino-sugars named purpurosamine A, B and C corresponding to gentamicins C1, C2a, and C1a respectively. The purpurosamines are shown to be members of the 2,6-diamino-2,3,4,6-tetradeoxyhexose family, a new class of naturally occurring monosaccharides.
Efficient construction of unit circular-arc models (2006)

In a recent paper, Durán, Gravano, McConnell, Spinrad and Tucker described an algorithm of complexity O(n2) for recognizing whether a graph G with n vertices is a unit circular-arc (UCA) graph. Furthermore the following open questions were posed in the above paper: (i) Is it possible to construct a UCA model for G in polynomial time? (ii) Is it possible to construct a model, whose extremes of the arcs correspond to integers of polynomial size? (iii) If (ii) is true, could such a model be constructed in polynomial time? In the present paper, we describe a characterization of UCA graphs which leads to linear time algorithms for recognizing UCA graphs and constructing UCA models. Furthermore, we construct models whose extreme of the arcs correspond to integers of size O(n). The proposed algorithms provide positive answers to the three above questions.
Malformations of angiogenesis in the low differentiated human carcinomas. immunohistochemical study. (2001)

Our previous observations showed that the perivascular mesenchyma of the thin-walled vessels (capillaries) in cancers may be the source of organ-specific stem cells. We suggested that the cells forming vascular channels in altered stroma participate in the tumor development. This study was designed to examine the distribution of the vessels and their appearance in the breast, lung and colon cancers. Using immunohistochemical methods, we have shown that in the low differentiated tumors both CD31 and factor VIII antigens may be expressed in capillaries chiefly on the periphery of neoplastic foci. Many of these vessels were discontinuous, with interruptions or unformed tubules. Sporadically, CD31 protein and factor VIII antigens were not expressed in capillaries inside the very low differentiated cancer cases. It is difficult to assess by immunohistochemical means whether the vascular malformations are the primary or secondary phenomena in the malignancy and why these abnormalities were especially visible in some low differentiated cancers.
Lipid-Polymer Bilaminar Oxygen Nanobubbles for Enhanced Photodynamic Therapy of Cancer (2018)

Hypoxia in solid tumors may be a hindrance to effective treatments of tumors in achieving their therapeutic potential, especially for photodynamic therapy (PDT) which requires oxygen as the supplement substrate. Oxygen delivery using perfluorocarbon emulsions or lipid oxygen microbubbles has been developed as the agents to supply endogenous oxygen to fuel singlet oxygen generation in PDT. However, such methods suffer from premature oxygen release and storage issues. To address these limitations, we designed lipid-polymer bilaminar oxygen nanobubbles with chlorin e6 (Ce6) conjugated to the polymer shell as a novel oxygen self-supplement agent for PDT. The resultant nanobubbles possessed excellent stability to reduce the risk of premature oxygen release and were stored as freeze-dried powders to avoid shelf storage issues. In vitro and in vivo experimental results demonstrated that the nanobubbles exhibited much higher cellular uptake rates and tumor targeting efficiency compared to free Ce6. Using the oxygen nanobubbles for PDT, a significant enhancement of therapeutic efficacy and survival rates was achieved on a C6 glioma-bearing mice model with no noticeable side effects, owing to the greatly enhanced singlet oxygen generation powered by oxygen encapsulated nanobubbles.
Solvent-free mechanochemical synthesis of dithiophosphonic acids and corresponding nickel(II) complexes (2017)

GRAPHICAL ABSTRACT
Graft polymerization of methyl methacrylate onto polyvinyl alcohol using Ce4+ initiator (1998)

Methyl methacrylate (MMA) was graft polymerized onto polyvinyl alcohol (PVA) using ceric ammonium sulfate as an initiator in aqueous medium. The grafting efficiency of MMA was studied as a function of monomer and initiator concentration, time, pH, and temperature. Grafting efficiency was found to be optimum at a particular level of ceric ammonium sulfate (3.16 × 10−3 mol L−1), PVA (1.0 g L−1), MMA (0.469 mol L−1), H2SO4 (0.188 mol L−1), temperature (45°C), and time (4 h). The probable reasons for the influence of reaction variable on the observed trend of MMA towards grafting have been discussed. Acid hydrolysis and infrared spectroscopy were used for the confirmation of graft copolymer formation. Thermogravimetric analysis of PVA and a representative graft copolymer were studied. The solubility/swellability and the gelatinization characteristics are also reported. © 1998 John Wiley & Sons, Inc. J. Appl. Polym. Sci. 70: 523–527, 1998
Characteristics of emitted fly ash and trace elements from utility boilers fired with pulverized coal (1984)

For the generation of electricity, coal becomes increasingly more important as a substitute for fuel oil. One of the consequences is an increase in the emission of fly ash and volatile (trace) particles; this emission has been studied for utility boilers that are fired with pulverized coal. The relevant parameters are: the type of boiler, the ash-collection devices employed, and the type of coal. The study concerns the distribution of trace particles over the various flows: from coal to furnace-bottom ash, pulverized fuel ashes (PFA) as collected in the successive compartments of the electrostatic precipitator (ESP), and the emitted ash (fly ash). It appears that, at increased particulate loadings in the flue gas after the ESP, the contribution of coarse particles will be larger and hence the enrichment of elements that submit to the vaporization-condensation mechanism, will be lower. There is evidence to show that enrichment cannot only be studied from the fly ash, but also from the trends of trace-element concentration in the PFA collected in the successive compartments of the ESP. In this study, the volatile elements' concentrations in the fly ash are about twice as high as in the PFA from the last ESP compartment. Therefore, the elemental composition of the PFA collected in the last ESP compartment is indicative of the composition of the emitted fly ash.