gebhart/patrepeval huggingface dataset:
Regulation of peripheral node addressin in tumor-associated CD31+ vascular endothelial cells. (2016)

Abstract Naïve T cells use peripheral node addressin (PNAd), normally found on lymph node (LN) high endothelial venules (HEV), to traffic into lymphoid organs. We previously reported that the vasculature of murine tumors expressed PNAd and CCL21, which supports infiltration and intratumoral activation of naïve T cells, in turn delaying tumor growth. PNAd expression on tumor vasculature depends on effector CD8+ T cells that secrete LTα3, which signals through TNFR on tumor-associated CD31+ endothelial cells. PNAd expression is low on tumor vasculature, suggesting that it might be regulated differently than PNAd on HEV. Using RT-qPCR, we found that most of the enzymes responsible for PNAd synthesis are expressed comparably in CD31+ cells from tumors and LN. However, the primary sulfotransferase responsible for the generation of the 6-sulfo sialyl Lewis X structure on PNAd HEV, GlcNAc6ST-2, is minimally expressed in tumors. Tumor CD31+ cells expressed equivalent levels of GlcNAc6ST-1, suggesting that it is responsible for low-level PNAd expression. Of the scaffolding proteins that make up PNAd, GlyCAM-1 was minimally expressed in tumor CD31+ cells, whereas all others were expressed comparably. To identify the components of PNAd biosynthesis in tumor CD31+ cells that are controlled by LTα3-TNFR signaling, we evaluated tumors from WT and TNFR1/2KO mice. GlyCAM-1 was the only assessed PNAd component that was ablated in TNFR1/2KO tumors. However, based on transcript levels, GlyCAM-1 accounts for only about 10% of the PNAd scaffolding proteins in tumor CD31+ cells. This suggests that TNFR signaling regulates additional PNAd biosynthetic components yet to be examined, or that it regulates one or more components at the level of protein translation.
Electromagnetic immunity of infusion pumps to GSM mobile phones: a systematic review. (2007)

Electromagnetic interference with life-sustaining medical care devices has been reported by various groups. Previous studies have demonstrated that volumetric and syringe pumps are susceptible to false alarm buzzing and blocking, when exposed to various electromagnetic sources. The risk of electromagnetic interference depends on several factors such as the phone-emitted power, distance and carrier frequency, phone model and antenna type. The main recommendations and the relevant harmonized standard are also reported and discussed. >From the data available in literature emerges that, for distances lower than 1 m there is a non negligible risk of electromagnetic interferences, although significant differences exists in the reported minimum distances. Interference effects clinically relevant for the patients are rare. No permanent damage to the pumps has been ever reported, although in several cases intervention of personnel is required to resume normal operation.
Results of Gamma-Ray Measurements from a Recent Demonstration for Russian Technical Experts (2001)

In August 2001, a group of U.S. technical experts demonstrated an Attribute Measurement System with an Information Barrier (AMSIB) for a delegation of Russian technical experts. The purpose of the demonstration was to show that attributes of a classified plutonium item of potential interest to arms control and nonproliferation transparency regimes could be ascertained without releasing any sensitive information. For this demonstration, both gamma-ray and neutron attributes were determined. We consider only the gamma-ray attributes here. The specific plutonium attributes measured were the isotopic ratio of {sup 240}Pu to {sup 239}Pu, the ''age'' of the plutonium (time elapsed since the most recent chemical purification of the plutonium), and the absence of plutonium oxide in the item's storage container. In this paper, we briefly review the technologies employed for the attribute measurements used in the gamma-ray portion of the demonstration, concentrating on the results of the test measurements of the isotopic and age attributes made on unclassified items.
Molecular Cloning of Paramyosin, a New Allergen of <i>Anisakis simplex</i> (2000)

We describe the primary structure of a paramyosin of A. simplex. It can be considered as an allergen based on its IgE binding capacity. We suggest that the recombinant protein does not maintain the complete allergenic properties of the native paramyosin, considering its lower IgE binding capacity of the recombinant protein. However, both proteins have the same specific IgE inhibition capacity. The recombinant protein can be produced in large quantities in E. coli. We propose the term Ani s 2 for this allergen.
Establishing a 3D ex vivo culture system for investigations of bone metabolism and biomaterial interactions. (2007)

A 3D load-providing culture system is presented, which enables human cancellous bone explants to be studied ex vivo. Bone integrity and activity was demonstrated during 2 to 3 weeks of culture. Viable osteocytes and bone specific markers could be identified. Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies.
Prolonged oxidative stress and delayed tissue repair exacerbate acetaminophen-induced liver injury in aged mice (2020)

The liver gradually loses its regenerative capabilities with aging. However, it remains unknown whether aging affects drug-induced liver injury. Here, we used acetaminophen induced acute liver injury model to compare tissue injury and regeneration of aged mice (>80 weeks old) with young ones (8-10 weeks old). The mortality of aged mice after acetaminophen injury was higher than that of young mice. Transient increase of serum GOT and decrease of reduced glutathione (GSH) were not returned to original levels in aged mice even at 48 hours. In addition, <i>Foxm1b</i> and its targets <i>Ccnd1</i> and <i>Cdk1</i> were upregulated in young but not in aged mice after 48 hours. Moreover, an apoptosis-related gene, <i>Cidea,</i> was upregulated specifically in aged livers, which was consistent with increased number of TUNEL<sup>+</sup> hepatocytes. Unexpectedly, damaged hepatocytes were retained in aged liver tissue, which may be caused by impaired recruitment of macrophages to the damaged area, without increases in <i>Ccl2</i> after acetaminophen injury. Collectively, prolonged oxidative stress due to delayed recovery of GSH and the retention of damaged hepatocytes may suppress tissue repair and hepatocyte proliferation, resulting in exacerbation of acetaminophen injury in aged mice. Thus, aging is a risk factor conferring susceptibility against drug-induced liver injury.
HGG-37. PAEDIATRIC GLIOBLASTOMA CELLS SHOW CRITICAL DEPENDENCIES ON EPIGENOMIC AND EPITRANSCRIPTOMIC CONTROL OF GENE EXPRESSION BY H3.3G34R/V MUTATIONS (2020)

Abstract H3.3G34R/V mutations are restricted to glioblastomas of the cerebral hemispheres, and occur predominantly in adolescents and young adults. We had previously shown these mutations to result in a global re-organisation of the activating mark H3K36me3 to drive transcription of key developmental transcription factors and oncogenes such as MYCN, however the precise mechanism was unclear. Using multiple H3G34R/V samples and ChIP-seq with antibodies specific to both wild-type and mutant histone H3.3, we show a high degree of incorporation of mutant histone into nucleosomes, with only a minority (&amp;lt;15%) remaining wild-type only. Heterogenous G34-mutant nucleosomes displayed significantly elevated H3K36me3 binding, the majority apparently in trans to the mutation on the wild-type H3.3, and expression signatures associated with chromatin modification, cell cycle progression, DNA repair and gene transcription. Super-enhancer analysis by H3K27ac ChIP-Seq highlighted lineage-dependent transcription factors and previously identified targets MYCN and NOTCH1 (both stabilised by FBXW7, down-regulated by loss of chromosome 4q), as well as specific H3K36 lysine demethylases and splicing factors. Whole-genome CRISPR-Cas9 screening of patient-derived H3.3G34R/V cells identified critical dependencies on these latter targets, in addition to a general essentiality for genes involved in RNA processing. Assessment of RNA methylation by MeRIP-seq revealed a strong concordance of m6A-modified RNA and H3K36me3 binding, with differentially modified transcripts in mutant cellsassociated with the 3’-UTR but also the promoter and gene bodies. These data highlight the critical nature of the epitranscriptome in H3.3G34R/V-mutant paediatric glioblastoma, and highlight novel targets for therapeutic intervention.
Orchestrating Resource Management in LTE-Unlicensed Systems With Backhaul Link Constraints (2019)

Long term evolution (LTE)-unlicensed, an extension of LTE Advanced to unlicensed spectrum, can provide high performance and seamless user experience. To reap the full benefits of the LTE-unlicensed deployment, efficient resource allocation and interference management are critical to ensuring a harmonious coexistence between LTE-unlicensed and WiFi systems. In this paper, we study a resource orchestration scheme for an LTE-unlicensed network where small cells share the same unlicensed spectrum with a WiFi system. An optimization problem for channel and power allocations is formulated to maximize the overall network utility, which is an NP-hard problem. The problem is constrained on meeting the desired data rate demands of the served small-cell users, the capacity-limited backhaul links, and the maximum tolerable interference at the WiFi access point. To solve this challenging problem, a distributed solution based on Lagrangian relaxation is proposed to assist the LTE-unlicensed network in making decisions on channel allocation and transmit power. Furthermore, low-complexity solutions are devised upon applying the one-to-one matching game theory. The simulation results with practical parameter settings show that the proposed algorithms converge to the suboptimal solution after a small number of iterations in the considered examples.
A Model to Predict Survival Following Liver Retransplantation (1999)

In the current era of critical-organ shortage, one of the most controversial questions facing transplantation teams is whether hepatic retransplantation, which has historically been associated with increased resource utilization and diminished survival, should be offered to a patient whose first allograft is failing. Retransplantation effectively denies access to orthotopic liver transplantation (OLT) to another candidate and further depletes an already-limited organ supply. The study group was comprised of 1,356 adults undergoing hepatic retransplantation in the United States between 1990 and 1996 as reported to the United Network for Organ Sharing (UNOS). We analyzed numerous donor and recipient variables and created Cox proportional-hazards models on 900 randomly chosen patients, validating the results on the remaining cohort. Five variables consistently provided significant predictive power and made up the final model: age, bilirubin, creatinine, UNOS status, and cause of graft failure. Although both hepatitis C seropositivity and donor age were significant by univariate and multivariate analyses, neither contributed independently to the estimation of prognosis when added to the final model. The final model was highly predictive of survival (whole model chi2 = 139.63). The risk scores for individual patients were calculated, and patients were assigned into low-, medium-, and high-risk groups (P <.00001). The low degree of uncertainty in the probability estimates as reflected by confidence intervals, even in our high-risk patients, underscores the applicability of our model as an adjunct to clinical judgment. We have developed and validated a model that uses five readily accessible "bedside" variables to accurately predict survival in patients undergoing liver retransplantation.
Using apodization functions to reduce sidelobes in rugate filters (1989)

Appending gradient-index matching regions and apodization, which is an amplitude modulation of the rugate sinusoidal index profile, are two effective means to reduce and nearly eliminate the sidelobes. When a combination of these methods is used in the rugate design, the resulting filter will have good sidelobe suppression both near to and far from the stopband and will have high reflectance in the stopband.
Inhibition by bestatin of a mouse ascites tumor dipeptidase (1989)

Bestatin, [(2S,3R)-3-amino-2-hydroxy-4-phenyl-butanoyl]-L-leucine, a known inhibitor of aminopeptidases, is shown to be a potent linear competitive inhibitor (KI,2.7 nM) of a dipeptidase purified from Ehrlich-Lettré hyperdiploid mouse ascites tumor cells. This inhibition can be classified as "slow binding" but not as "tight binding." Substrate protects the enzyme from bestatin inhibition when enzyme and inhibitor are in approximately equimolar concentrations. Addition of substrate (6 mM) partially (by about 20%) reverses dipeptidase inhibition by bestatin, but the time required for maximum recovery depends on the nature of the substrate. Substrates with lower Km (0.28-1.4 mM) values that exhibit substantial substrate inhibition require longer times (23-65 min) than those with higher Km values that show little substrate inhibition. Substrates with Km values higher than 1.5 mM do not reverse inhibition. The inhibition of the tumor dipeptidase by bestatin has been compared with inhibition by a variety of inhibitors of other Zn-metallo-proteolytic enzymes. These inhibitors were far less potent (KI, 0.063-10 mM), indicating a difference between the tumor dipeptidase and other enzymes of that class. Our results are discussed in terms of a postulated model of the bestatin molecule in the active site of the tumor dipeptidase, an enzyme which has not been studied by x-ray crystallographic means. The phenyl group of bestatin is placed in a hydrophobic pocket that is external but adjacent to the active site of the tumor dipeptidase. The shape of this pocket, as it appears from our results plus modeling, is such that only certain R groups of substrate can fit. The existence of such a pocket might explain the differential effect of substrates in the reversal of bestatin inhibition of the dipeptidase and also might explain substrate inhibition by misalignment of R groups into this pocket.
Neutrophil Proteases Associated with Amyloid Fibrils (1993)

We found significant amounts of enzymatic activity characteristic of the human neutrophil proteases, elastase and cathepsin G, associated with isolated amyloid fibrils from patients with five different types of systemic amyloidosis. Amyloid deposits in tissue sections from the patients with amyloid A, amyloid transthyretin and amyloid beta 2-microglobulin amyloidosis also stained positive with antiserum to elastase and cathepsin G. Elastase and cathepsin G, found in the azurophilic granules of the neutrophil and, to a lesser extent, the monocyte, may become associated with amyloid precursor proteins before or during the formation of amyloid fibrils. This may occur in an extracellular inflammatory microenvironment or in a phagolysosome and play a role in the formation of the fibrils.
Toxicity of Chronic Spinal Analgesia in a Canine Model: Neuropathologic Observations with Dezocine Lactate (1990)

The chronic spinal toxicity of dezocine lactate was investigated in mongrel dogs. Dogs received chronic intrathecal infusion from implanted infusion pumps for 28-136 days. Infusion of saline via intrathecal catheters produced leptomeningeal fibrosis, sometimes with spinal cord compression. Dezocine lactate infusion, in addition to similar leptomeningeal changes, was also associated with severe parenchymal lesions in all cases. The exact cause of this toxicity cannot be specifically assigned; potential contributing factors include catheter-induced reaction, pH of the drug, lactate concentration, osmolality and the pharmacologic agent itself. Leptomeningeal reaction in control dogs limits the value of chronic intrathecal dog models for assessment of spinal drug toxicity.
A Novel Parallel 8B/10B Encoder: Architecture and Comparison with Classical Solution (2018)

8B/10B is an encoding technique largely used in different communication protocols, with several advantages such as zero DC bias. In the last years transmission rates have grown rapidly, thus the need of encoders with better performance in terms of throughput, area and power consumption raised rapidly. In this article we will present and discuss the architecture of two symbols parallel encoder, comparing it with a classical pipelined solution.
Intrinsic subtypes and therapeutic decision-making in hormone receptor-positive/HER2-negative metastatic breast cancer with visceral crisis: A case report (2022)

This clinical case confirms in the real-world setting that non-luminal subtypes can be found in HR+/HER2-neg disease and may have potential therapeutic implications in the metastatic setting. It also questions the recommendation of upfront chemotherapy in the case of a visceral crisis in the era of CDKi-based regimens. These issues merit further evaluation in prospective and larger studies.
Computer-based grading of haematoxylin-eosin stained tissue sections of urinary bladder carcinomas (2001)

The proposed image analysis system may be of value to the objective assessment of the malignancy of urine bladder carcinomas, since it relies on nuclear parameters that are employed in visual grading and their prognostic value has been proved.
The non‐glyceride saponifiables of shea butter (1977)

Abstract Triterpene alcohols and sterols were found to be present in a commercial sample of shea butter (the seed fat of Butyrospermum parkii , Sapotaceae) as esters of both cinnamic and fatty acids. These non‐glyceride saponifiables represented 6% by weight of the shea butter under study, whereas free triterpene alcohols and sterols accounted for only 1 % by weight. Cinnamic acid was found neither in the free form, nor esterified to glycerol. Saponification of the non‐glyceride saponifiables yielded the triterpene alcohols α‐amyrin, butyrospermol, lupeol, β‐amyrin and germanicol as major components. Other alcohols identified were parked, 24‐methylenelanost‐9(11)‐en‐3‐ol, dammaradienol, 24‐methylenedammarenol and the two sterols α‐spinasterol and Δ 7 ‐stigmastenol. Esterified to these alcohols were cinnamic ( trans and cis ), palmitic, stearic, oleic, linoleic and arachidic acids. The fatty acid composition of the non‐glyceride saponifiables is different from that of the glycerides. No selectivity was observed in the esterification of these fatty acids by triterpene alcohols and sterols. Investigation of the products from acid catalysed transmethylation has led to two new experimental findings: (i) the difference in reaction rate of fatty acid and cinnamic acid esters and (ii) the formation of methoxylated artifacts from alcohols having unsaturation, in the C 17 side chain, between C 24 and C 25 by the addition of methanol across the double bond. Analysis of the lipids extracted from shea kernels confirmed that the cinnamic acid esters were only present as non‐glyceride saponifiables.
Increased adenosine concentration in blood from ischemic myocardium by AICA riboside. Effects on flow, granulocytes, and injury. (1989)

Morbidity and mortality from acute coronary artery occlusion may be reduced if local myocardial adenosine concentration is augmented because 1) coronary collateral blood flow during ischemia increases with adenosine infusion, and 2) granulocytes that accumulate in the microcirculation during ischemia are, to a large extent, inhibited by adenosine from generating superoxide anion free radicals, from adhering to vascular endothelium, and from damaging endothelial cells in culture. Using a cultured lymphoblast model system, we found that 5-amino-4-imidazole carboxamide (AICA) riboside enhanced adenosine accumulation during ATP catabolism. Therefore, AICA riboside pretreatment was used in canine myocardium to selectively increase adenosine concentration in the ischemic area during 1 hour of ischemia. At 5 minutes of ischemia, endocardial flow to ischemic myocardium in saline-treated and AICA riboside-treated dogs was 0.06 +/- 0.03 and 0.34 +/- 0.11 ml/min/g, respectively (p less than 0.01); flow to nonischemic myocardium was not affected. Ventricular tachycardia and premature ventricular depolarizations were significantly attenuated in the AICA riboside-treated dogs. Blood pressure and heart rate were not affected by AICA riboside. In venous blood from ischemic tissue, adenosine increased from undetectable levels (less than 0.01 microM) to 0.22 +/- 0.08 microM in saline and 1.79 +/- 0.06 microM in AICA riboside-treated dogs, respectively (p less than 0.001). Coronary vein inosine concentrations were greater in saline than in AICA riboside-treated dogs. In separate in vitro studies, AICA riboside did not alter the removal rate of adenosine from canine blood. Indium-labeled granulocyte accumulation was significantly less in ischemic myocardium in AICA riboside-treated compared with saline-treated dogs. In addition, adenosine, but not AICA riboside, inhibited in vitro canine granulocyte superoxide production. We conclude that AICA riboside given before myocardial ischemia augments adenosine concentration, decreases arrhythmias, decreases granulocyte accumulation, and improves collateral flow to ischemic myocardium. One of the beneficial mechanisms could be an increased production of adenosine rather than inosine from ATP catabolism that causes vasodilation and inhibition of granulocytes. We propose a new hypothesis regarding regulation of the inflammatory reaction to ischemia in the microcirculation. Adenosine, in addition to its vasodilator action, is an anti-injury autacoid that links ATP catabolism to inhibition of granulocyte adherence, microvascular obstruction, and superoxide anion formation.
ChemInform Abstract: INFRARED SPECTRA OF WATER IN CRYSTALLINE HYDRATES, KSNCL3.H2O, AN UNTYPICAL MONOHYDRATE (1974)

Abstract Die IR‐Spektren von polykristallinen KSnCla ° H 2 O ‐Proben bei verschiedenen partiellen Deuterationsgraden werden zwischen 4000 und 300 cm ‐1 bei Temperaturen zwischen 30 und ‐160°C aufgenommen.
ChemInform Abstract: Enantiospecific Synthesis of Quisqualic Acid. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Utilization of nitrogenous compounds by oral bacteria (1990)

In terms of the crucial acid-base balance in dental plaque, the bacterial catabolism of nitrogenous compounds, such as peptides and amino acids, is of importance because the end-products can raise plaque pH. Of particular significance is the fermentation of arginine by bacteria such as Streptococcus sanguis, a numerically important plaque organism. Aspects of the uptake of this amino acid were studied and it was also shown the organism can obtain arginine from small peptides, since it possesses cell-associated exo-peptidases. Furthermore, it could grow in media containing whole protein (casein), or one of its fractions, as the sole source of organic nitrogen. The studies thus showed that S. sanguis is well equipped, in terms of endo- and exo-peptidase activities, to obtain the metabolically important arginine from whole protein. It is suggested that knowledge of this type should lead to a better understanding of overall plaque metabolism--of relevance to both cariogenic and periodontopathic plaques.
Demosaicking methods for Bayer color arrays (2002)

The <i>Journal of Electronic Imaging</i> (JEI), copublished bimonthly with the Society for Imaging Science and Technology, publishes peer-reviewed papers that cover research and applications in all areas of electronic imaging science and technology.