gebhart/patrepeval huggingface dataset:
Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys (2019)

Spermatogonial stem cell (SSC) transplantation therapy is a promising strategy to renew spermatogenesis for prepubertal boys whose fertility is compromised. However, propagation of SSCs is required due to a limited number of SSCs in cryopreserved testicular tissue. This propagation must be done under xeno-free conditions for clinical application. SSCs were propagated from infant testicular tissue (7 mg and 10 mg) from two boys under xeno-free conditions using human platelet lysate and nutrient source. We verified SSC-like cell clusters (SSCLCs) by quantitative real-time polymerase chain reaction (PCR) and immune-reaction assay using the SSC markers undifferentiated embryonic cell transcription factor 1 (UTF1), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), GDNF receptor alpha-1 (GFRα-1) Fα and promyelocytic leukaemia zinc finger protein (PLZF). The functionality of the propagated SSCs was investigated by pre-labelling using green fluorescent Cell Linker PKH67 and xeno-transplantation of the SSCLCs into busulfan-treated, therefore sterile, immunodeficient mice. SSC-like cell clusters (SSCLCs) appeared after 2 weeks in primary passage. The SSCLCs were SSC-like as the UTF1, UCHL1, GFRα1 and PLZF were all positive. After 2.5 months' culture period, a total of 13 million cells from one sample were harvested for xenotransplantation. Labelled human propagated SSCs were identified and verified in mouse seminiferous tubules at 3-6 weeks, confirming that the transplanted cells contain SSCLCs. The present xeno-free clinical culture protocol allows propagation of SSCs from infant boys.
2017 End of Year Podcast (2017)

An end of year compilation of conversations with various NASA scientists, engineers, and researchers throughout 2017 on the NASA in Silicon Valley Podcast.
Antibody Persistence Following Preexposure Regimens of Cell‐Culture Rabies Vaccines: 10‐Year Follow‐Up and Proposal for a New Booster Policy (1998)

Subjects (n = 312) received either the human diploid cell rabies vaccine (HDCV) or the purified Vero cell rabies vaccine (PVRV) according to either two-injection (days 0 and 28) or three-injection (days 0, 7, and 28) primary regimens. They received a booster injection at 1 year. Rabies antibody levels were measured after the primary series and the booster and then each year for the next 10 years. The results confirm the superior long-term immunogenicity of the three-injection over the two-injection protocol. HDCV and PVRV in three doses were equally immunogenic. A booster injection at 1 year provides long-term seroconversion (titer > or = 0.5 IU/mL). Antibody titers 2 weeks after the 1-year booster allowed prediction of long-term immunity. Good responders, with titers > or = 30 IU/mL, were protected for at least 10 years. An algorithm for differentiation between good responders and poor responders with respect to vaccine booster strategies is proposed.
Refractoriness to Renal Effects of Aldosterone in the Golden Hamster (1971)

The effect of adrenalectomy and replacement thei*apies on survival, plasma electrolytes, and the excretion of water and saline loads was studied in golden hamsters. The pattern of response was in several respects different from that of other commonly studied mammals. Adrenalectomy caused death usually within less than a week, associated with a mild hyperkalemia but no definite hyponatremia. The availability of saline drinking solutions caused only slight if any life extension. Aldosterone extended or maintained life in small doses (2 or more μg/ day), as did cortisol at high dosage (ca. 0.5 mg/ day). In saline loading tests suitable for bioassay of aldosterone in rats this steroid caused no Na retention in hamsters, nor did it inhibit the natriuresis induced by cortisol. Hydrochlorothiazide was weakly natriuretic but not kaliuretic at the dosage used—an effect not modified by aldosterone. In the special conditions of the Kagawatype assay, aldosterone caused a weak but not dose-dependent Na retention. Aldosterone at high doses and cortisol at all active doses caused natriuresis and kaliuresis. In adrenalectomized animals not loaded with fluid, both aldosterone and cortisol at high doses caused hypernatremia, but only the former completely relieved hyperkalemia. On a Na-deficient diet the hypernatremic effects of aldosterone and cortisol, singly or combined, were essentially abolished. In contrast to effects on Na metabolism, the qualitative action of these adrenal steroids on K metabolism were similar to those in other mammals. (Endocrinology89: 63, 1971)
Attention is All We Need: Nailing Down Object-centric Attention for\n Egocentric Activity Recognition (2018)

In this paper we propose an end-to-end trainable deep neural network model\nfor egocentric activity recognition. Our model is built on the observation that\negocentric activities are highly characterized by the objects and their\nlocations in the video. Based on this, we develop a spatial attention mechanism\nthat enables the network to attend to regions containing objects that are\ncorrelated with the activity under consideration. We learn highly specialized\nattention maps for each frame using class-specific activations from a CNN\npre-trained for generic image recognition, and use them for spatio-temporal\nencoding of the video with a convolutional LSTM. Our model is trained in a\nweakly supervised setting using raw video-level activity-class labels.\nNonetheless, on standard egocentric activity benchmarks our model surpasses by\nup to +6% points recognition accuracy the currently best performing method that\nleverages hand segmentation and object location strong supervision for\ntraining. We visually analyze attention maps generated by the network,\nrevealing that the network successfully identifies the relevant objects present\nin the video frames which may explain the strong recognition performance. We\nalso discuss an extensive ablation analysis regarding the design choices.\n
Phase 1 clinical trial of the PI3Kδ inhibitor YY-20394 in patients with B-cell hematological malignancies (2021)

YY-20394, an oral phosphatidylinositol 3-kinase delta (PI3Kδ) inhibitor, was investigated in a first-in-human study of patients with relapsed or refractory B-cell malignancies. During dose escalation, 25 patients received 20-200 mg of YY-20394 daily. The primary outcome measures were tolerability and dose-limiting toxicity (DLT). The secondary outcomes were pharmacokinetic parameters, progression-free survival (PFS) and the objective response rate (ORR). Since no patients experienced DLT, the maximum tolerated dose (MTD) was not reached. The majority (≥ 5%) of drug-related adverse events were ≥ grade III, being neutropenia (44.0%), pneumonia (16.0%), hyperuricemia (12.0%), lymphocythemia (8.0%), leukopenia (8.0%) and pneumonitis (8.0%). The overall ORR was 64.0% (95% confidence interval (CI): 45.2, 82.8%) including 5 patients with complete remission (CR), 11 with partial remission (PR), 2 with stable disease (SD) and 7 with progressive disease (PD), while the disease control rate (DCR) was 72.0% (95% CI: 54.4, 89.6%). The ORR of 10 patients with follicular lymphoma was 90%. The median PFS time was 255 days. One PR patient with chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) who received 40 mg q.d. had a durable response of around 36 months. The median PFS time of 10 patients with follicular lymphoma was 300 days. A recommended phase 2 dose of 80 mg q.d. was established. Considering that YY-20394 was well-tolerated with promising preliminary efficacy, further development is warranted.Trial registration clinicaltrials.gov, NCT03757000, retrospectively registered, November 28, 2018, https://clinicaltrials.gov/ct2/show/NCT03757000?term=NCT03757000&draw=2&rank=1 .
The Application Of Photoconductive Detectors To The Measurement Of X-Ray Production In Laser Produced Plasmas (1988)

Photoconductive detectors (PCDs) offer an attractive alternative for the measurement of pulsed x-rays from laser produced plasmas. These devices are fast (FWHM &apos;000 ps), sensitive and simple to use. We have used InP, GaAs, and Type IIa diamond as PCDs to measure x-rays emission from 100 eV to 100 keV. Specifically, we have used these detectors to measure total radiation yields, corona temperatures, and hot electron generated x-rays from laser produced plasmas.
Total Synthesis of (−)-Tetrazomine. Determination of the Stereochemistry of Tetrazomine and the Synthesis and Biological Activity of Tetrazomine Analogues (2002)

The first total synthesis of the potent antitumor antibiotic (-)-tetrazomine has been accomplished. A new method for the formation of the allylic amine precursor to an azomethine ylide has been developed and exploited in an efficient [1,3]-dipolar cycloaddition to afford the key tetracyclic intermediate used in the synthesis of (-)-tetrazomine. Several analogues of tetrazomine have been synthesized and tested for antimicrobial and biochemical activity.
Analytical Characterization of Fatty Acids Composition of Bioactive Stem Oil of Maytenus royleanus by Gas Chromatography Mass Spectrometry (2012)

Abstract The significant inhibition and selectivity for human solid tumor cell by oily fraction of Maytenus royleanus , was subjected to GC‐MS analysis for determination of its chemical constituents. GC‐MS profile of methyl ester derivatives of fatty acids, showed that Palmitic acid (35.41%), Oleic acid (10.91%), Stearic acid (5.31%), Margaric acid (5.13%), Behenic acid (5.18%) and Hexanoic acid (4.97%) were the major components in the isolated oily fraction, while rest of the other fatty acids were present in minor concentration. The literature revealed that no such work has been done for the determination of fatty acids in M. royleanus stem oil.
Identification and Purification of Diphosphoinositol Pentakisphosphate Kinase, Which Synthesizes the Inositol Pyrophosphate Bis(diphospho)inositol Tetrakisphosphate (1998)

Diphosphoinositol pentakisphosphate (PP-IP5) and bis(diphospho)inositol tetrakisphosphate (bis-PP-IP4) were recently identified as inositol phosphates which possess pyrophosphate bonds. The molecular mechanisms that regulate the cellular levels of these compounds are not yet characterized. To pursue this question, we have previously purified an inositol hexakisphosphate (IP6) kinase from rat brain supernatants [Voglmaier, S. M., et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 4305-4310]. We now report the identification and purification of another novel kinase, diphosphoinositol pentakisphosphate (PP-IP5) kinase, which uses PP-IP5 as a substrate to form bis(diphospho)inositol tetrakisphosphate (bis-PP-IP4) in soluble fractions of rat forebrain. The purified protein, a monomer of 56 kDa, displays high affinity (Km = 0.7 microM) and selectivity for PP-IP5 as a substrate. The purified enzyme also can transfer a phosphate from bis-PP-IP4 to ADP to form ATP. This ATP synthase activity is an indication of the high phosphoryl group transfer potential of bis-PP-IP4 and may represent a physiological role for PP-IP5 and bis-PP-IP4.
A new on-chip voltage regulator for high density CMOS DRAMs (2003)

The authors report on new voltage regulator circuitry used in a 4 Mb CMOS DRAM. The DRAM accepts a 5 V external power supply and generates 3.3 V on chip. The chip photomicrograph is provided with a magnified portion of the regulator. The internal voltage V/sub INT/ is regulated at 3.3 V (25 degrees C), while V/sub DD/ varies from 3.3 to 6.2 V. V/sub INT/ presents a positive temperature coefficient, which is adjustable, to compensate the higher cell leakage and the slowdown of MOSFET operations at higher temperature. While V/sub DD/ is raised above 6.3 V, the regulator enters the burn-in mode where V/sub INT/ follows a 2/3 V/sub DD/ curve, which gives an exact internal burn-in voltage as desired. This internal burn-in voltage is insensitive to temperature and process variations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Collision‐induced decompositions of [M – H]− and [M + Li]+ ions from fast atom bombardment of dinucleoside phenylphosphonates (1993)

Abstract The collision‐induced decompositions of the [M – H] − and [M + Li] + ions of a few dinucleoside phenylphosphonates were studied using fast atom bombardment and linked scanning at constant B/E . Deprotonation takes place on the base or sugar moieties. The [M – H] − ion decomposes mainly by cleavage on either side of the phosphonate linkage, leading to the formation of mononucleotide fragment ions and also by cleavage of the basesugar bond. Rupture of the 3′‐phosphonate bond is preferred. Unlike the normal charged nucleotides, these neutral nucleotides do not eliminate a neutral base from the [M – H] − ion. However, the mononucleotide fragment ions which can have the charge on the phosphorus oxygen eliminate neutral bases by charge‐remote fragmentation. The 4,4′‐dimethoxytrityl (DMT)‐protected nucleotides show the additional fragmentation of loss of DMT. Li + attachment can occur at several sites in the molecule. As observed for the [M – H] − ion, the major cleavage occurs on either side of the phosphonate bond in the fully deprotected nucleotides, cleavage of the ester bond on C(3′) being preferred. Cleavage of the 5′‐phosphonate bond is not observed in the DMT‐protected nucleotides. Many of the fragmentations observed can be explained as arising from charge‐remote reactions.
SHORTWAVE LIMIT OF INFRARED INTERSUBBAND QUANTUM CASCADE LASERS (2001)

The feasibility and fundamental difficulty in developing the infrared intersubband quantum cascade lasers in shorter wavelength range were discussed theoretically. The band offsets of InAs/AlSb on GaSb substrate, InAs/Al 0.6 Ga 0.4 Sb on GaSb substrate, and In 0.53 Ga 0.47 As/In 0.52 Al 0.48 As on InP substrate, and the confined states of their quantum wells with and without an applied electric field were analyzed and calculated by using the fundamental assumption of model solid theory, strained band structure theory, empirical two band model for non parabolic band, and methods of propagation matrix and laminar approach. It was found that the greatest energy difference between subband edges can not exceed 56~62% of the conduction band offset, and will be decreased further by the indirect valley. A designed structure of intersubband quantum cascade laser consisting of 250 coupled quantum wells in 25 periods under electric field 100kV/cm for emitting the shortest wavelength 2.88μm was proposed.
A framework to evaluate short- and long-term performance of roller compacted concrete pavements (2022)

This paper presents a framework to evaluate the short-term (i.e., 28 days) and long-term (i.e., 30 years) performance of Roller Compacted Concrete (RCC) pavement under traffic loading, materials, and environmental impacts. The framework assesses the short- and long-term damages of RCC pavement considering various factors including the aggregate gradation, the compaction level, the environmental residual stresses, and the geometrical inputs. It calculates the stresses at four critical locations in the pavement, which subsequently can be used to calculate the damage at these locations. The traffic model employed in the framework incorporates either simple traffic inputs (i.e., AADT, truck percent, growth function, and rate) or detailed traffic characteristics. This framework facilitates the effective evaluation of different aspects of RCC pavement design. The case study presented in this paper shows that the variability in the materials constituents, materials density, and environmental impacts can be compensated by increasing the designed thickness of the RCC pavement. This finding can be used to i) prioritize the mix design of RCC materials based on design inputs and, ii) quantify the optimum RCC pavement thickness required to minimize the effect of materials and construction factors in the field.
Reduction in Serum Aquaporin-4 Antibody Titers During Development of a Tumor-Like Brain Lesion in a Patient With Neuromyelitis Optica: A Serum Antibody–Consuming Effect? (2015)

Neuromyelitis optica (NMO) is an inflammatory demyelinating disease of the CNS with severe involvement of the optic nerve and spinal cord. Highly specific serum IgG autoantibodies (NMO-IgG) that react with aquaporin-4 (AQP4), the most abundant CNS water channel protein, are found in patients with NMO. However, in vivo evidence combining the results of AQP4 antibody serum levels and brain pathology is lacking. We report a patient with NMO whose AQP4 antibody levels decreased simultaneously with clinical deterioration caused by the development of a tumor-like brain lesion. In the seminecrotic biopsied brain lesion, there was activated complement complex, whereas only very scattered immunoreactivity to AQP4 protein was detectable. The decrease in serum AQP4 antibody levels and the loss of AQP4 in the tumor-like lesion could represent a "serum antibody-consuming effect" during lesion formation.
Change impact identification in object oriented software maintenance (1994)

In the object-oriented (OO) paradigm, new features (such as encapsulation, aggregation, inheritance, polymorphism and dynamic binding) introduce new problems in software testing and maintenance. One of them is the difficulty of identifying the affected components (such as classes) when changes are made in object-oriented class libraries or programs. This paper discusses the types of code changes in an object-oriented class library, and provides an automated solution to identify different kinds of code changes and their impact. In addition, an OO software maintenance environment that implements the research result is described. Our experience with the environment prototype shows promising results.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Femtosecond lentotomy: generating gliding planes inside the crystalline lens to regain accommodation ability (2010)

Abstract Based on Helmholtz Theory for accommodation the increasing sclerosis of lens nucleus and cortex is the main cause for the developments of presbyopia. Existing therapies, however, do not reverse the stiffness of the crystalline lens and thus do not regain real accommodation ability. A new approach to restore the flexibility of the lens could be realized by photodisruption using ultrafast laser pulses. This process, known as fs‐lentotomy, was used to create micro‐incisions which act as gliding planes inside the crystalline lens without opening the eye globe. (© 2010 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Electronic spectra of 7,14‐dithioketo‐5,7,12,14‐tetrahydroquinolino‐[2,3‐b]‐acridine in solution and in the solid state (1994)

Abstract 7,14‐Dithioketo‐5,7,12,14‐tetrahydroquinolino‐[2,3‐b]‐acridine (DTQ) is a thionated derivative of quinacridone (QA) that is known as a red pigment. Thionation of QA brings about an intense near‐IR absorption in the solid state which is of practical importance for GaAsAl‐laser printers and optical information storage systems. The electronic properties of DTQ have been investigated in solution and in the solid state in order to characterize the near‐IR absorption from the standpoint of intermolecular hydrogen bonding. The electronic state of the DTQ‐chromophore is found to be most sensitively affected by an environment of proton acceptors which interact with the NH group of the chromophore. In solution, the hydrogen bonds between the NH group and the O atom of a solvent cause a bathochromic shift by 5 – 15 nm relative to the absorption spectrum in dioxane. This is interpreted as being due to electron transfer from the proton to the nitrogen atom, thereby increasing the electron density in the chromophore. A similar increase in electron density is also operative in the solid state through the intermolecular hydrogen bonds between the NH group of one DTQ and the S atom of the neighboring molecule. This is mainly responsible for the bathochromic displacement of DTQ in going from solution to the solid state. The near‐IR absorption is, however, not explicable in terms of the hydrogen bonding interaction alone. Another intermolecular interaction, such as π‐π interactions along the stacking axis, appears to be operative that might lead to a near‐IR absorption.
CS2N3, A Novel Pseudohalogen (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTCS2N3, A Novel PseudohalogenMargaret-Jane Crawford, Thomas M. Klapötke, Peter Klüfers, Peter Mayer, and Peter S. WhiteView Author Information Department of Chemistry, University of Munich D-81377 Munich, Germany Department of Chemistry, University of North Carolina Chapel Hill, North Carolina 27599-3290 Cite this: J. Am. Chem. Soc. 2000, 122, 37, 9052–9053Publication Date (Web):August 30, 2000Publication History Received26 April 2000Published online30 August 2000Published inissue 1 September 2000https://pubs.acs.org/doi/10.1021/ja001457bhttps://doi.org/10.1021/ja001457brapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views548Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Anions,Crystal structure,Nitrogen,Physical and chemical processes,X-rays Get e-Alerts
Processing Factors Involved in Sintering β‐Si 3 ,N 4 ,‐Based Ceramics in an Air Atmosphere Furnace (2009)

This chapter contains sections titled: Introduction Experimental Procedures Results and Discussion Conclusions Acknowledgements References
Synthesis of α-Methylene-γ-lactone Fused to Seven, Eight, and Fourteen-membered Carbocycle through Intramolecular Cyclization of Functionalized Allylsilane with Acid Chloride (1998)

Abstract α-Methylene-γ-lactone fused to cycloheptane ring was synthesized by intramolecular cyclization of 8-(ethoxycarbonyl)- or 8-(acetoxymethyl)-9-(trimethylsilyl)non-7-enoyl chloride followed by lactonization. α-Methylene-γ-lactone fused to cyclooctane and cyclotetradecane ring were also synthesized from 9-(acetoxymethyl)-10-(trimethylsilyl)dec-8-enoyl chloride and 15-(acetoxymethyl)-16-(trimethylsilyl)hexadec-14-enoyl chloride, respectively.