gebhart/patrepeval huggingface dataset:
DNA sequence of the serum opacity factor of group A streptococci: identification of a fibronectin-binding repeat domain (1995)

The serum opacity factor (SOF) is a group A streptococcal protein that induces opacity of mammalian serum. The serum opacity factor 22 gene (sof22) from an M type 22 strain was cloned from an EMBL4 library by screening for plaques exhibiting serum opacity activity. DNA sequencing yielded an open reading frame of 3,075 bp. Its deduced amino acid sequence predicts a protein of 1,025 residues with a molecular weight of 112,735, a size that approximates that of the SOF22 protein isolated from both the original streptococcal strain and Escherichia coli harboring the cloned sof22 gene. The molecule is composed of three domains: an N-terminal domain responsible for the opacity reaction (opacity domain), a repeat domain with fibronectin-binding (Fn-binding) activity, and a C-terminal cell attachment domain. The C-terminal end of SOF22 is characterized by a hexameric LPXTGX motif, an adjacent hydrophobic region, and a charged C terminus, which are the hallmarks of cell-bound surface proteins found on nearly all gram-positive bacteria. Immediately upstream of this cell anchor region, SOF22 contains four tandem repeat sequence blocks, flanked by prolinerich segments. The repeats share up to 50% identity with a repeated motif found in other group A streptococcal Fn-binding proteins and exhibit Fn-binding activity, as shown by subcloning experiments. According to deletion analysis, the opacity domain is confined to the region N terminal to the repeat segment. Thus, SOF22 is unique among the known Fn-binding proteins from gram-positive bacteria in containing an independent module with a defined function in its N-terminal portion. Southern blot analysis with a probe from this N-terminal region indicates that the opacity domain of SOF varies extensively among different SOF-producing M types.
Nonuniform Fast Fourier Transform on Tpus (2021)

This work presents a parallel algorithm for implementing the nonuniform Fast Fourier transform (NUFFT) on Google's Tensor Processing Units (TPUs). TPU is a hardware accelerator originally designed for deep learning applications. NUFFT is considered as the main computation bottleneck in magnetic resonance (MR) image reconstruction when k-space data are sampled on a nonuniform grid. The computation of NUFFT consists of three operations: an apodization, an FFT, and an interpolation, all being formulated as tensor operations in order to fully utilize TPU's strength in matrix multiplications. The implementation is with TensorFlow. Numerical examples show 20x ~ 80x acceleration of NUFFT on a single-card TPU compared to CPU implementations. The strong scaling analysis shows a close-to-linear scaling of NUFFT on up to 64 TPU cores. The proposed implementation of NUFFT on TPUs is promising in accelerating MR image reconstruction and achieving practical runtime for clinical applications.
Production of δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine by entrapped ACV-synthetase fromStreptomyces clavuligerus (1995)

delta-(L-alpha-Aminoadipyl)-L-cysteinyl-D-valine (ACV)-synthetase from Streptomyces clavuligerus was studied under conditions that enabled the reuse of the enzyme. Coupling of ACV-synthetase to DEAE-Trisacryl and aminopropyl-glass resulted in an immobilized enzyme product of little or no catalytic activity. However, an enzyme reactor was designed by physical confinement of partially-purified ACV-synthetase in an ultrafiltration cell. This system was stimulated by phosphoenolpyruvate at lower concentrations of ATP, an effect not observed with purified enzyme. Up to 30% conversion of the limiting substrate, cysteine, to ACV occurred under semi-continuous conditions. Reaction products were investigated as potential inhibitors: AMP was the most inhibitory, but only when used at concentrations in excess of those produced in reaction mixtures. Under a nitrogen atmosphere, both product and enzyme stabilities were greatly improved and the enzyme retained 45-65% of its initial activity after five uses at room temperature during a 24-h period. Extrapolations based on these data suggest that 1.3 g partially purified enzyme (0.13 U g-1) would be capable of producing 411 mg of ACV in a 1-L reaction mixture in this period.
ChemInform Abstract: MACRO RINGS. 48. TRANSANNULAR EFFECTS OF ACIDITY OF HETEROANNULARLY DISUBSTITUTED (2.2.) PARACYCLOPHANE (1982)

Abstract Die pK a ‐Werte der 4‐Carboxy‐ oder 4‐Aminoparacyclophane (I) werden durch Carboxylatg o‐Nitro‐ oder o‐Br‐ bzw. durch NH 2 ‐ oder m‐NHz‐Substitution am zweiten Ring erhöht, durch die übrigen genannten Substituenten in 2‐Methoxyethanol/H2O verringert.
Electron spin resonance studies of extended excess electron states in frozen solutions of alkali metals in hexamethylphosphoramide (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectron spin resonance studies of extended excess electron states in frozen solutions of alkali metals in hexamethylphosphoramideRon Catterall and Peter P. EdwardsCite this: J. Phys. Chem. 1975, 79, 26, 3018–3024Publication Date (Print):December 1, 1975Publication History Published online1 May 2002Published inissue 1 December 1975https://pubs.acs.org/doi/10.1021/j100593a045https://doi.org/10.1021/j100593a045research-articleACS PublicationsRequest reuse permissionsArticle Views39Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Circhoral Oscillations of Plasma LH Levels in the Ovariectomized Rhesus Monkey 1 , 2 (1970)

Blood samples were withdrawn at 10-, 20- or 30-min intervals for 6–12 hr from 13 ovariectomized and 7 intact female rhesus monkeys bearing indwelling cardiac catheters and restrained in primate chairs. Plasma LH and GH concentrations were determined by radioimmunoassay. Twenty-four experiments in ovariectomized animals revealed a striking rhythmic pattern in plasma LH levels having a period of approximately 1 hr, which was asynchronous with variations in plasma GH concentrations when these occurred. The LH oscillations, which had an excursion of 40–92 % of the mean hormone concentration, occurred with a mean period of 75 min and a mode of 60 min. In a typical rhythmic sequence, LH levels increased from nadir to peak during one 10-min sampling interval and decayed exponentially for the next 60 min. The t½ calculated from 19 such decay curves based on 10-min sampling intervals was 72 min, which is similar to the estimated t½ of exogenous LH in intact females. Moreover, there was a significant positive correlation between the duration of the decay and the magnitude of the next peak. Such oscillations did not occur in intact females. It is suggested that these circhoral (about an hr) oscillations in plasma LH concentrations, occasioned by ovariectomy, represent pulsatile discharges of LH from the pituitary and probably account for the greater part of the elevated LH plasma levels seen in ovariectomized animals. (Endocrinology87: 850, 1970)
The Utility of Four Biochemical Markers in the Triage of Chest Pain Patients (2000)

Four biochemical markers, creatine kinase (CK)-MB isoenzyme, myoglobin, myosin light chains and troponin I, were studied in 1,338 patients presenting to the emergency department with chest pain suggestive of coronary artery disease (CAD). One hundred and eighty-seven patients had an acute myocardial infarction (MI). At least one of the four markers was over the threshold on the first sample in 78% of MI patients, as compared to only 40% with an elevated CK-MB. After 4 h, 88% had at least one marker elevated. None of the 69 patients with atypical chest pain, no history of CAD, no markers over threshold on the first sample and a normal electrocardiogram had an acute MI or unstable angina. If we had discharged this group, we would have saved USD 264,000, estimating a cost of USD 2,000 per day. Using four biochemical markers improved the early diagnosis of CAD and may help identify groups suitable for early discharge.
[Mechanisms of anti-atherogenic action of probucol]. (1999)

Probucol has been recognized to have antioxidant properties as well as lipid-lowering effects, and contribute to prevention of atherosclerosis. Pharmacologically, this agent has shown several unique actions, and a lot of investigations have been reported regarding the mechanisms. Recently, it has been reported that probucol increases selective uptake of high density lipoprotein cholesterol esters by the medium of the class B, type I scavenger receptor (SR-BI), and that antioxidant potent may inhibit the restenosis of post coronary angioplasty. This review attempts to focus on the mechanism of antioxidant and HDL-lowering effects of probucol.
Monte Carlo simulation-based design study of a LSO-LuAP small animal PET system (2003)

Positron emission tomography (PET) scanners dedicated to small animal imaging should combine very high sensitivity (>2%) and resolution (<2 mm) to achieve image quality comparable to that in human. A promising way to meet these criteria is to develop compact phoswich detector modules with depth-of-interaction (DOI) capability to allow increased sensitivity due to longer crystals without impairing spatial resolution. We are currently exploring this concept within the framework of the European Crystal Clear Collaboration by designing a small animal PET scanner based on L(Y)SO-Lu(Y)AP detector modules coupled to multichannel photomultiplier (MCPMT) tubes. The goal of this work was to perform simulation studies to evaluate the influence of the geometrical design (crystal length, ring diameter, detector packing fraction) on resolution and sensitivity performances of a one-ring prototype.
Språk, kultur a handel i ett svenskt perspektiv (2011)

Sverige ar ett exportland med lang tradition av handel med omvarlden. For att kunna utnyttja vara absoluta och komparativa fordelar galler det att vi har god kunskap om vad som paverkar var handel. ...
Effect of sacral neuromodulation on the rectum (2005)

SNM affects rectal sensory perception, but further research is required to clarify the mechanism.
Preparation and Characterization of Chitosan/SiO<SUB>2</SUB> Composite Films (2010)

Composite films made from chitosan, plasticizer, and incorporated silica nanoparticles were evaluated for their mechanical properties, thermal stability, water permeability, and surface characteristics. The influence of the chitosan amino group protonation upon the observed silica effects was also analyzed. Only slight differences between most of the properties of composite films and those of the biopolymer alone were observed. The lack of complete and uniform dispersion of the silica particles within the biopolymer matrix and the absence of relevant polymer-filler interactions in such composite membranes lead to a low level of interaction between the filler and the polymer, and only a reduced effect of the filler on the film properties. The silica effects worthy to mention are the decrease in Young's modulus and the increase in film elongation, and the slight influence on surface hydrophobicity and water vapour permeability, which were dependent on the neutralization pos-treatment process.
When should treatment of AL amyloidosis start at relapse? Early, to prevent organ progression (2019)

Abstract This article has a companion Counterpoint by Sanchorawala.
Effects of Nonlinearities on Subsonic Aerodynamic Center (2008)

A refined mathematical definition for the aerodynamic center of a wing or complete airplane is presented, which allows for inclusion of the trigonometric and aerodynamic nonlinearities. From this definition, analytical relations are developed for both the axial and vertical positions of the aerodynamic center at any angle of attack, independent of whether or not the nonlinearities are included. Results show that, when all nonlinearities are included, the position of the aerodynamic center can change with angle of attack. Because the traditional approximation provides only a fixed axial coordinate, this analysis provides two additional pieces of information about the aerodynamic center, that is, its vertical coordinate and its movement. Two examples are presented, which separate these two effects. The first example uses computational fluid dynamics to show that, when the effects of the nonlinearities are combined with wing sweep at high angles of attack below stall, the aerodynamic center of a planar wing moves significantly aft and below the wing. The second example, which uses an approximate closed-form solution for two lifting surfaces, emphasizes the importance of knowing the vertical position of the aerodynamic center and shows that, in the absence of sweep, its movement with angle of attack is slight.
Ganoderma lucidum immunomodulatory protein(Lz-8) expressed in Pichia pastoris and the identification of immunocompetence. (2009)

Fungi immunoregulatory proteins family is effective in immunological regulation and anti-tumor. We used Pichia pastoris expression system for recombinant expression of Lz-8, an immunomodulatory protein isolated from fruiting body of Ganoderma lucidum. The Gs115 (mut+) strains of P. pastoris was used as host cells. PCR and sequencing of DNA showed that Lz-8 cDNA was successfully integrated into the P. pastoris genome. Electrophoresis (SDS-PAGE), matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) and immunological techniques were used to identify recombinant Lz-8 (rLz-8). Lz-8 expressed in Escherichia coli, the Pichia system requires further optimization to obtain more active fungi immunomodulatory protein. Lz-8 was expressed in P. pastoris successfully, and polyacrylamide gel electrophoresis in the presence of SDS-PAGE gave a single band with an apparent Mr=14,000 D. MALDI-TOF-MS also showed that molecular weight of rLz-8 was 12,722 D. Aggregation was observed from sheep red blood cells in the presence of purified rLz-8 within the concentration range of 12.5-50 microg/mL. However, no aggregation was seen at concentration greater than 50 microg/mL for any type of human red blood cell. The dose at 0.5 mg/kg of rLz-8 induced macrophage cytophagocytesis, and set interferon as control at 0.5 mg/kg. These results suggested that active and stable rLz-8 was obtained in P. pastoris expression system.
Surface evaluation of UV-degraded contamination (1992)

Three different areas of work were accomplished under this contract: (1) contamination testing and evaluation; (2) UV irradiation testing; and (3) surface evaluation testing. Contamination testing was generally performed in the In-Situ Contamination Effects Facility at Marshall Space Flight Center (MSFC). UV irradiation testing was also performed primarily at MSFC, utilizing facilities there. Finally, the surface evaluation was done at facilities at UAH Center for Applied Optics.
Dielectric relaxation and magnetic behavior of bismuth-substituted yttrium iron garnet (2011)

Y3−xBixFe5O12 (x = 0–1.0) was prepared by solid state reaction. X-ray diffraction showed a gradual increase of lattice parameter with the Bi concentration, indicating that Bi had been incorporated into the lattice. Dielectric responses of the samples at room temperature increased initially at low Bi (x &amp;lt; 0.4) and then decreased rapidly at high Bi (x ≥ 0.6). Temperature dependence of dielectric constant showed a huge steplike increase at the temperatures between 20 and 200 °C, increasing with Bi. X-ray photoelectron spectroscopy revealed the presence of Fe2+ and Bi2+ in the samples, which might be responsible for the dielectric behaviors. An increase of coercivity and Curie temperature with Bi was observed. This was accompanied by a reduction of the saturation magnetization, whose variation with T follows a modified Bloch law.
Automatic Singing Performance Evaluation for Untrained Singers (2009)

In this letter, we present an automatic approach of objective singing performance evaluation for untrained singers by relating acoustic measurements to perceptual ratings of singing voice quality. Several acoustic parameters and their combination features are investigated to find objective correspondences of the perceptual evaluation criteria. Experimental results show relative strong correlation between perceptual ratings and the combined features and the reliability of the proposed evaluation system is tested to be comparable to human judges.
Selected Genetic Disorders Affecting Ashkenazi Jewish Families (2007)

Ashkenazi Jews of Central and Eastern European ancestry have a disproportionately high prevalence of several autosomal recessive genetic disorders. This article describes these 9 disorders and their genetic inheritance patterns: Bloom syndrome; Canavan disease; cystic fibrosis; familial dysautonomia; Fanconi anemia; Gaucher disease; Mucolipidosis IV; Niemann-Pick disease; and Tay-Sachs disease. Genetic testing, counseling, and family planning options for the at-risk population are described. The role of the community health nurse is addressed.
Is the sequence-specific binding of aminoacyl-tRNAs by EF-Tu universal among bacteria? (2011)

Three base pairs in the T-stem are primarily responsible for the sequence-specific interaction of tRNA with Escherichia coli and Thermus thermophilus EF-Tu. While the amino acids on the surface of EF-Tu that contact aminoacyl-tRNA (aa-tRNA) are highly conserved among bacteria, the T-stem sequences of individual tRNA are variable, making it unclear whether or not this protein-nucleic acid interaction is also sequence specific in other bacteria. We propose and validate a thermodynamic model that predicts the ΔG° of any tRNA to EF-Tu using the sequence of its three T-stem base pairs. Despite dramatic differences in T-stem sequences, the predicted ΔG° values for the majority of tRNA classes are similar in all bacteria and closely match the ΔG° values determined for E. coli tRNAs. Each individual tRNA class has evolved to have a characteristic ΔG° value to EF-Tu, but different T-stem sequences are used to achieve this ΔG° value in different bacteria. Thus, the compensatory relationship between the affinity of the tRNA body and the affinity of the esterified amino acid is universal among bacteria. Additionally, we predict and validate a small number of aa-tRNAs that bind more weakly to EF-Tu than expected and thus are candidates for acting as activated amino acid donors in processes outside of translation.
Cyclopentadien-Metallkomplexe, ein neuer Typ metallorganischer Verbindungen (1952)

Das äußerst stabile, unpolare Dicyclopentadienyl-eisen, Fe II (C 5 H 5 ) 2 , stellt sich als neuartiger Typ eines Durchdringungskomplexes dar. Das zentrale Eisen(II)-Ion besitzt hier durch oktaedrisch-koordinative Bindung an die beiden dreizähligen, aromatisierten, parallel gegenüberstehenden Cyclopentadienyl-Anionen Kryptonkonfiguration. Chemische,magnetische und röntgenographische Befunde bestätigen diese Auffassung. Auch in den komplexen Kationen [Fe III (C 5 H 5 ) 2 ] + und [Co III (C 5 H 5 ) 2 ] + , welche als schwerlösliche Reinedceate isoliert wurden, erscheint das Cyclopentadienyl gleichartig gebunden.
Development of high chroma and dichromatic luster pigments by synthesis of Li2CoTi3O8 onto the surface of mica (1996)

高彩度二色性光輝性粉体の開発を目的として, 混合スピネル構造をもっチタン系複合酸化物Li2CoTi3O8を雲母へ被覆することを検討した。その結果, 雲母チタンを原料としてCoO/TiO2およびLi2O/TiO2のモル比を調整することによって, 青色および緑色の外観色を示す干渉性粉体を得ることができた。XRDによる構造解析の結果, 雲母表面にLi2CoTi3O8の生成が確認された。また青色と緑色の外観色の違いは結晶形の違いによるものではなく, 格子定数の違いによるものであった。このLi2CoTi3O8被覆雲母は原料とする雲母チタンの干渉色を調整することによって, 様々な二色性を有する安定性の高い高彩度光輝性粉体として得ることができた。
The Chemotherapeutic Drug 5-Fluorouracil Promotes PKR-Mediated Apoptosis in a p53- Independent Manner in Colon and Breast Cancer Cells (2011)

The chemotherapeutic drug 5-FU is widely used in the treatment of a range of cancers, but resistance to the drug remains a major clinical problem. Since defects in the mediators of apoptosis may account for chemo-resistance, the identification of new targets involved in 5-FU-induced apoptosis is of main clinical interest. We have identified the ds-RNA-dependent protein kinase (PKR) as a key molecular target of 5-FU involved in apoptosis induction in human colon and breast cancer cell lines. PKR distribution and activation, apoptosis induction and cytotoxic effects were analyzed during 5-FU and 5-FU/IFNα treatment in several colon and breast cancer cell lines with different p53 status. PKR protein was activated by 5-FU treatment in a p53-independent manner, inducing phosphorylation of the protein synthesis translation initiation factor eIF-2α and cell death by apoptosis. Furthermore, PKR interference promoted a decreased response to 5-FU treatment and those cells were not affected by the synergistic antitumor activity of 5-FU/IFNα combination. These results, taken together, provide evidence that PKR is a key molecular target of 5-FU with potential relevance in the clinical use of this drug.
High field magnetic resonance imaging of the periodontal space: First experimental results (2010)

Poster: ECR 2010 / C-1697 / High field magnetic resonance imaging of the periodontal space: First experimental results by: C. Gaudino, S. Heiland, M. Pham, R. Cosgarea, M. Bendszus, S. Rohde; Heidelberg/DE
Smoking Promotes Subclinical Atherosclerosis in Apparently Healthy Men (2012)

Significant 2-year progression of subclinical atherosclerosis was associated with continuous smoking and LDL-C. This was only partly moderated in past smokers despite complete reversal of inflammatory activation, suggesting another crucial factor for inhibiting accelerated progression of subclinical atherosclerosis in men.
Pharmacological Properties and Functional Role of Kslow Current in Mouse Pancreatic β-Cells (2005)

The pharmacological properties of slow Ca(2+)-activated K(+) current (K(slow)) were investigated in mouse pancreatic beta-cells and islets to understand how K(slow) contributes to the control of islet bursting, [Ca(2+)](i) oscillations, and insulin secretion. K(slow) was insensitive to apamin or the K(ATP) channel inhibitor tolbutamide, but UCL 1684, a potent and selective nonpeptide SK channel blocker reduced the amplitude of K(slow) tail current in voltage-clamped mouse beta-cells. K(slow) was also selectively and reversibly inhibited by the class III antiarrythmic agent azimilide (AZ). In isolated beta-cells or islets, pharmacologic inhibition of K(slow) by UCL 1684 or AZ depolarized beta-cell silent phase potential, increased action potential firing, raised [Ca(2+)](i), and enhanced glucose-dependent insulin secretion. AZ inhibition of K(slow) also supported mediation by SK, rather than cardiac-like slow delayed rectifier channels since bath application of AZ to HEK 293 cells expressing SK3 cDNA reduced SK current. Further, AZ-sensitive K(slow) current was extant in beta-cells from KCNQ1 or KCNE1 null mice lacking cardiac slow delayed rectifier currents. These results strongly support a functional role for SK channel-mediated K(slow) current in beta-cells, and suggest that drugs that target SK channels may represent a new approach for increasing glucose-dependent insulin secretion. The apamin insensitivity of beta-cell SK current suggests that beta-cells express a unique SK splice variant or a novel heteromultimer consisting of different SK subunits.