gebhart/patrepeval huggingface dataset:
“Kick-Starting” oxetane photopolymerizations (2014)

In the presence of small amounts of 2,2-dialkyl-, 2,2,3-trialkyl-, or 2,2,3,3-tetraalkyl substituted epoxides such as isobutylene oxide, 1,2-limonene oxide, and 2,2,3,3,-tetramethyl oxirane, the photoinitiated cationic ring-opening polymerizations of 3,3-disubstituted oxetanes are dramatically accelerated. The acceleration affect was attributed to an increase in the rate of the initiation step of these latter monomers. Both mono- and disubstituted oxetane monomers are similarly accelerated by the above-mentioned epoxides to give crosslinked network polymers. The potential for the use of such “kick-started” systems in applications such as coatings, adhesives, printing inks, dental composites and in three-dimensional imaging is discussed. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 2934–2946
Flexible all fiber Fabry-Perot filters based on superimposed chirped fiber Bragg gratings with continuous FSR tunability and its application to a multiwavelength fiber laser (2007)

We proposed and experimentally demonstrate a simple and flexible scheme of all-fiber Fabry-Perot filter with continuous free spectral range tunability exploiting a superimposed chirped fiber Bragg grating. Then, we apply the proposed continuously tunable multichannel filter to a multiwavelength SOA ring laser at room temperature. The specially designed apparatus can induce the symmetrical strain gradient and modify the chirp ratio along the superimposed chirped fiber Bragg grating attached onto a flexible cantilever beam. Then the free spectral range of the all-fiber Fabry-Perot filter can be continuously controlled by the variation of reflection chirp bandwidth of the superimposed chirped fiber Bragg grating without center wavelength shift, which depends on the moving direction of translation stage. The proposed scheme can provide the effective multichannel filter with continuous free spectral range tunability. We successfully achieve the stable operation of a multiwavelength SOA laser with eleven lasing channels based on the proposed flexibly tunable multichannel filter at room temperature.
Measurement of Renal Red Cell and Plasma Transit Times in Acute Renal Failure (1963)

SummaryA method of measuring of red cell and plasma transit times through the kidney has been developed. The technic involves simultaneous rapid sampling of arterial and renal venous blood during the equilibration time across the kidney following a peripherally injected intravenous bolus of Cr51 labelled red cells and HI131SA. In control subjects the mean red cell transit time averaged 6.7 ± 0.79 seconds and mean HI131SA transit time 9.22 ± 0.83 seconds. In 5 patients in the oliguric stage of acute renal failure the mean red cell transit time was significantly reduced to 4.7 ± 0.74 seconds while the HI131SA transit time averaged 9.56 ± 1.1 seconds. The significance of these results in relation to the existence of a cortical shunt for red cells in acute renal failure is discussed.
COUP-TF (chicken ovalbumin upstream promoter transcription factor)-interacting protein 1 (CTIP1) is a sequence-specific DNA binding protein (2002)

Chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting proteins 1 and 2 [CTIP1/Evi9/B cell leukaemia (Bcl) l1a and CTIP2/Bcl11b respectively] are highly related C(2)H(2) zinc finger proteins that are abundantly expressed in brain and the immune system, and are associated with immune system malignancies. A selection procedure was employed to isolate high-affinity DNA binding sites for CTIP1. The core binding site on DNA identified in these studies, 5'-GGCCGG-3' (upper strand), is highly related to the canonical GC box and was bound by a CTIP1 oligomeric complex(es) in vitro. Furthermore, both CTIP1 and CTIP2 repressed transcription of a reporter gene harbouring a multimerized CTIP binding site, and this repression was neither reversed by trichostatin A (an inhibitor of known class I and II histone deacetylases) nor stimulated by co-transfection of a COUP-TF family member. These results demonstrate that CTIP1 is a sequence-specific DNA binding protein and a bona fide transcriptional repressor that is capable of functioning independently of COUP-TF family members. These findings may be relevant to the physiological and/or pathological action(s) of CTIPs in cells that do not express COUP-TF family members, such as cells of the haematopoietic and immune systems.
Dual role of Zn2+ as inhibitor and activator of fructose 1,6-bisphosphatase of rat liver. (1976)

At neutral pH, Zn2+ is a potent and specific inhibitor of rat liver fructose 1,6-bisphosphatase (EC 3.1.3.11; D-fructose-1,6-bisphosphate 1-phosphohydrolase). Inhibition by Zn2+ is uncompetitive with respect to the activating cations Mg2+ and Mn2+, and the kinetic data suggest that the enzyme possesses a distinct high-affinity binding site for Zn2+, with Ki of approximately 0.3 muM. At higher concentrations (about 10(-5) M) Zn2+, and to a lesser extent Co2+, function as activating cations. Binding studies show that the enzyme binds two equivalents of Zn2+ per subunit; one equivalent is partially displaced by Mg2+ and is presumably bound to the site for activating cations. A second equivalent binds to the high-affinity site, presumably identical to the inhibitory site. The results suggest that Zn2+ functions as an allosteric regulator, and that the commonly observed activation of fructose 1,6-bisphosphatase at neutral pH by EDTA, histidine, and other chelators is due to removal of endogenous Zn2+ by these agents.
349 ZYX is a Novel Candidate Gene in Moyamoya Pathogenesis (2023)

INTRODUCTION: Moyamoya disease (MMD) is characterized by progressive bilateral stenosis in the proximal branches of the internal carotid arteries with compensatory angiogenesis. It is common in Japanese population; accounting for one fifth of pediatric stroke cases associated with arteriopathies. Although the etiology remains largely unknown, genetic factors have been implicated in the pathophysiology of MMD. METHODS: We performed gene-based mutational burden analysis using WES results of 23 MMD patients and a control cohort of 8380 Japanese individuals obtained from jMorp (8.3 kjpn). We performed a case-control analysis by comparing the enrichment of rare (Japanese minor allele frequency &lt;2.5e-4) and deleterious variants including loss of function (LoF) variants (stop gain, start loss, frameshift, splice acceptor and splice donor) and missense variants annotated as “deleterious” in MetaSVM (D-Mis). RESULTS: We identified ZYX gene, encoding a focal adhesion molecule Zyxin, top significantly enriched gene in MMD cohort (two tailed Fisher's P = 1.52E-5; OR 77.55 [95% CI 13.91–291.69]), with three individuals were found to carry a heterozygous LoF and two missense mutations in ZYX gene. ZYX expression was found above 96th percentile for an ‘arterial-enrichment ratio’ calculated using GtEX portal, suggesting its critical role in vascular cell types. CONCLUSIONS: In this study, we identified ZYX as a novel candidate gene in Moyamoya pathogenesis. Zyxin is a known focal adhesion molecule involved in mechanosensation in the vasculature, while its implication in MMD has not been previously reported.
Radiation-guided anti-P-selectin antibody for tumor targeted imaging and therapy (2007)

4187 Purpose/Objective : Radiation induces translocation of P-selectin from storage reservoirs within the tumor endothelium to the luminal surface of microvasculature. This radiation-inducible expression of P-selectin on tumor-associated endothelial cells can be developed for tumor-targeted therapy and imaging. We developed specific antibodies against P-selectin, and tested the feasibility of imaging and therapy of irradiated lung tumors in vivo using targeted therapeutic radioisotopes. Materials & Methods : Single chain variable fragments (ScFv) of murine and human antibodies were selected by screening phage-displayed ScFv libraries on purified P-selectin. The selected ScFvs were prioritized by affinity (ELISA and FMAT), specificity (cell and tissue staining), and stability. Prioritized antibodies were engineered for conjugating with different carriers, expressed in E. coli and purified on affinity columns. The ScFvs were conjugated with chelating agents (DTPA and DOTA) and radioisotopes for imaging and therapy. These radioimmunoconjugates were injected into mice with irradiated lung tumors. Biodistribution of the ScFv-conjugates in vivo was monitored with near-infrared (NIR) imaging, gamma camera imaging, and micro-PET. Results: It was found that ScFv antibodies against P-selectin specifically bind to irradiated HUVEC cells in vitro , and target irradiated tumor xenografts in vivo . DTPA-conjugated ScFv loaded with radioisotope specifically and efficiently targeted irradiated tumor within hours as revealed with gamma camera imaging and micro-PET. ScFv were labeled with In-111 for initial imaging studies and with Cu-64 for subsequent imaging and therapy studies. Biodistribution and pharmacokinetics of therapeutic radioisotopes targeted to P-selectin in irradiated in vivo lung tumor models will be presented. Conclusions : P-Selectin is specifically induced by irradiation of tumor vascular endothelium. Screening of phage-displayed antibody fragments is an efficient means for selecting anti-P-selectin antibody with high affinity and specificity. Radiation-induced expression of P-selectin on tumor vascular endothelial cells can be utilized for imaging and therapy of tumors. Therapeutic radioimmunoconjugates targeting P-selectin can be used for imaging and therapy in irradiated in vivo lung tumor models.
Real-time nanometer-vibration measurement with a self-mixing microchip solid-state laser (2002)

Nanometer vibration analysis of a target has been demonstrated by a self-aligned optical feedback vibrometry technique that uses a laser-diode-pumped microchip solid-state laser. The laser output waveform, which was modulated through interference between a lasing field and an extremely weak (<- 100-dB) frequency-modulated (FM) feedback field, was analyzed by the Hilbert transformation to yield the vibration waveform of the target. Experimental signal characteristics have been reproduced by numerical simulations. Real-time vibration measurement has also been achieved with a simple FM demodulation circuit.
Bovine factor XIIa inhibitor (1993)

Bovine factor XIIa inhibitor was purified by an improved method employing affinity for heparin. N-terminal amino acid sequencing revealed a unique sequence without homology to any other known protein sequences. Peptide sequencing, however, showed that a part of the bovine factor XIIa inhibitor was homologous to human C1-inhibitor with a fraction of identical amino acid residues around 70%. Deglycosylation studies and carbohydrate analysis showed the presence of N- and O-linked carbohydrate. Bovine factor XIIa inhibitor did not inhibit plasma kallikrein and trypsin. The reactive site comprised an Arg-Asn bond, and represents the first example of asparagine as a P1' residue in Serpins with well documented inhibitory activity.
Molecular Characterization of a New Begomovirus Infecting Honeysuckle in Kobe,Japan (2005)

A virus (HY12) isolated from honeysuckle (Lonicera japonica Thunb.) collected in Kobe, Hyogo prefecture, Japan, showing symptoms of yellow vein mosaic, was characterized. HY12 was transmitted to Nicotiaua benthamiana by sap inoculation while the other honeysuckle isolates could not. The complete DNA-A and DNA-like molecules of the isolate were determined and found to comprise 2763 and 1262 nucleotides (nt), respectively, with a typical begomovirus genome organization. The DNA molecule had the conserved ORF ( C1) and satellite conserved region (SCR) typical of the Geminiviridae family containing a 5'-TMTATTAC-3' motif in the stem loop and the adenine-(A) rich region. Additionally, its entire sequence was interrupted at intervals wlth sequences that were not viral and whose origin was unknown. A comparison of the DNA-A sequences revealed that HY12 shared 740/0 with four other honeysuckle begomoviruses but 88-890/0 with tobacco begomoviruses found in Japan. The isolate shared up to 920/0 of the IR sequences with the honeysuckle begomoviruses but only 60-880/0 with those of tobacco. Phylogenetic analysis of full length DNA-A component as well as its CP clustered HY12 with the tobacco begomoviruses. uvl2 is an isolate of Howeysuckle yellow vein mosaic virus.
On-chip microlasers for biomolecular detection via highly localized deposition of a multifunctional phospholipid ink (2013)

We report on a novel approach to realize on-chip microlasers, by applying highly localized and material-saving surface functionalization of passive photonic whispering gallery mode microresonators. We apply dip-pen nanolithography on a true three-dimensional structure. We coat solely the light-guiding circumference of pre-fabricated poly(methyl methacrylate) resonators with a multifunctional molecular ink. The functionalization is performed in one single fabrication step and simultaneously provides optical gain as well as molecular binding selectivity. This allows for a direct and flexible realization of on-chip microlasers, which can be utilized as biosensors in optofluidic lab-on-a-chip applications. In a proof-of-concept we show how this highly localized molecule deposition suffices for low-threshold lasing in air and water, and demonstrate the capability of the ink-lasers as biosensors in a biotin-streptavidin binding experiment.
Authentication mechanisms in microprocessor-based local area networks (1989)

The problem of authenticating the users of a computer network in order to protect the shared resources against unauthorized use is discussed. Since intruders could enter the network and try to use services they have no right to access, the host implementing the service (or server) has to check the user's identity and access rights by searching in the relevant database. The author presents a method of carrying out such checks efficiently. The basic idea is that a suitable interface process is associated with each user-server connection in order to filter out unauthorized requests, thus implementing a sort of cache with parallel search where the working set of the whole database is stored and explored. The use of the interface process enables the system to exploit the hardware support for capability checking provided by new microprocessors. In particular, an implementation using iAPX432-based hosts is illustrated and performance issues are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
1'-Hydroxyeugenol- and coniferyl alcohol derivatives as effective inhibitors of 5-lipoxygenase and Cu(2+)-mediated low density lipoprotein oxidation. Evidence for a dual mechanism. (1994)

1'-Hydroxyeugenol- and epoxy-Z-coniferyl alcohol esters from Coreopsis species as well as synthetic derivatives of these natural compounds were examined as lipoxygenase inhibitors and as LDL (low density lipoprotein)-stabilizing agents. Most of the compounds displayed inhibitory activity on the formation of leukotrienes (LTB4 and LTC4) in a cellular (RBL-1 cells) assay as well as in a cell-free 5-lipoxygenase assay at concentrations of 4-24 mumol/l. No effect of selected compounds was observed on mammalian lipoxygenases with other specificity (12- and 15-lipoxygenase). The more lipophilic derivatives also effectively reduced Cu(2+)-mediated oxidation of LDL. The findings are discussed on the base of structure-activity relationships.
Co-immobilized poly(ethylene glycol)-block-polyamines promote sensitivity and restrict biofouling on gold sensor surface for detecting factor IX in human plasma (2014)

In order to detect an extremely low amount of human coagulation factor IX (FIX), poly(ethylene glycol) (PEG)/aptamer co-immobilized surface was constructed using original PEG-polyamine surface modification agents on surface plasmon resonance (SPR) sensor chip. Initially, a gold (Au) sensor chip of SPR was modified using poly(ethylene glycol)-b-poly[2-(N,N-dimethylamino)ethyl methacrylate] (PEG-b-PAMA) followed by treatment with SH-dT20 and was duplexed with anti-FIX aptamer extended using A24. Furthermore, the co-immobilization of pentaethylenehexamine-terminated poly(ethylene glycol) (N6-PEG) on the sensing surface completely quenched bio-fouling. On this dual tethered PEG-surface, we determined that the dissociation constant for FIX-aptamer interaction was 37 ± 10 pM, and the sensitivity of detection could reach up to 800 fM on using aptamer-FIX-antibody sandwich pattern detected by gold nanoparticle-conjugated anti-mouse antibody. We could detect FIX in the presence of abundant albumin. Furthermore, to mimic the actual detection of FIX in clinical samples, we demonstrated our experimental results with human blood plasma instead of FIX. Higher-sensitivity was attained because of dual polymers immobilized on Au surface, and this can emerge as a common strategy for any aptamer-protein interactions. The selective binding of aptamer in human blood plasma described here indicates the suitability of the present strategy for detection in clinically relevant samples.
A Well-Test Analysis Method Accounting for Pre-Test Operations (2003)

Summary In recent work, we have extended the classical radial flow model used in a standard pressure drawdown or buildup well-test analysis by incorporating an additional parameter characterizing the pumping rate prior to the test. By this means, we develop an approach for estimating the formation hydraulic properties in the vicinity of the wellbore from regular pumping rate-pumping pressure data. In this paper, the approach is established as a well-test analysis method by accomplishing two steps. First, five parameters, including the reservoir transmissivity, storativity, skin factor, and pre-test pumping rate, are determined from analysis of measured data. Second, the average reservoir pressure and drainage radius are determined from a calculation using parameters obtained in the first part.
Photonic bandgap crystal InGaAsP membrane microresonators (2002)

We have microfabricated 2-D photonic bandgap structures in a thin slab of dielectric material to define reflectors and high-Q microresonators. By selectively omitting holes from the two-dimensional photonic crystal, laser cavities and optical resonator switches can be defined. We have designed this structure with a finite difference time domain (FDTD) approach, and demonstrate some promising designs in InGaAs/InGaAsP multiple quantum well material with emission wavelength of 1.55 microns.
Analytical expressions for side fringing response and crosstalk with finite head and track widths (1977)

An analytical expression is derived that describes the response of a magnetic read head of finite width to a magnetized track of finite width shifted in the lateral direction. With this expression the side fringing response and crosstalk from adjacent tracks are calculated and a number of results is presented.
Real-time spectral CT thermometry via physical density for image-guided tumor ablation (2022)

Hepatocellular carcinoma, the fastest rising cause of cancer-related deaths, is commonly treated with percutaneous ablative therapies where tumor cells are destroyed once tissue temperatures reach a lethal threshold. However, high progression and recurrence rates post ablation suggest the need for intraprocedural temperature monitoring to ensure the lethal threshold (>60&deg;C) is reached and a sufficient safety margin is obtained. A previously developed model generates physical density maps from spectral CT data. These spectral physical density quantifications enable thermometry by taking advantage of the thermal volumetric expansion equation that relates the change in temperature to physical density changes. To validate the physical density model, an ex vivo bovine muscle was weighed and scanned on a clinical spectral CT scanner with different scanning parameter combinations (collimation, dose, helical/axial scans). Calculated mass from physical density maps and volume demonstrated high accuracy with a maximum percent error of 0.34% (&lt;1.1 grams for a345 gram sample) and minimal effects of scanning parameters. After validating the accuracy of the physical density maps, the muscle was subjected to heating and cooling while scanning to evaluate the relationship between physical density and temperature. Spectral results were continuously generated to calculate physical density maps at different temperatures. A linear relationship between change in temperature and change in physical density was established with strong correlation (R = 0.9781). The reflection of thermal volumetric expansion in physical density quantifications indicate its potential utility for providing real-time temperature feedback to interventional radiologists during ablative procedures for not only hepatocellular carcinoma, but also other types of malignancies.
Syrjäytymisvaarassa olevien vajaakuntoisten nuorten kuntoutustarpeen arviointi (2007)

Halonen J-P, Aaltonen T, Hamalainen A, Karppi S-L, Kaukinen J, Kervila A, Lehtinen M, Pere E, Puukka P, Siitonen V, Silvennoinen S, Talo S. Assessment of rehabilitation needs among handicapped young adults at risk of exclusion. Helsinki: The Social Insurance Institution, Finland. Social security and health reports 73, 2007. 89 pp. ISBN 978-951-669-736-2 (nid.), 978-951-669-737-9 (pdf). Fifty-eight young adults at risk of exclusion underwent a rehabilitation examination in the Social Insurance Institution’s rehabilitation centre (now Petrea). All of the participants had been diagnosed with physical, mental or social functional limitations preventing them from participation in the labour market. The rehabilitation examinations were performed by an interdisciplinary team comprising a physician, social worker, psychologist, nurse and physical therapist. An individual rehabilitation plan was drawn up for nearly all of the participants in a process that revealed extensive information about the personal background factors exposing the participants to a risk of exclusion. Based on the International Classification of Functioning, Disability and Health, an individual profile of functioning and disability was created for each participant. The profiles indicated a preponderance of psychological and social problems. However, the young adults themselves had a more positive view of their own situation than the interdisciplinary team. The project recommendations highlight the importance of the early recognition of needs, seamless cooperation between authorities, the appointment of a single body responsible for following up on the rehabilitation plan, and the development of new mental health services designed for young adults.