gebhart/patrepeval huggingface dataset:
Ambient Ionization Mass Spectrometry: Recent Developments and Applications (2019)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTAmbient Ionization Mass Spectrometry: Recent Developments and ApplicationsClara L. FeiderClara L. FeiderDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United StatesMore by Clara L. FeiderView Biography, Anna KriegerAnna KriegerDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United StatesMore by Anna KriegerView Biography, Rachel J. DeHoogRachel J. DeHoogDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United StatesMore by Rachel J. DeHoogView Biography, and Livia S. Eberlin*Livia S. EberlinDepartment of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States*E-mail: [email protected]More by Livia S. EberlinView Biographyhttp://orcid.org/0000-0002-3885-3215Cite this: Anal. Chem. 2019, 91, 7, 4266–4290Publication Date (Web):February 21, 2019Publication History Published online21 February 2019Published inissue 2 April 2019https://pubs.acs.org/doi/10.1021/acs.analchem.9b00807https://doi.org/10.1021/acs.analchem.9b00807review-articleACS PublicationsCopyright © 2019 American Chemical SocietyRequest reuse permissionsArticle Views12022Altmetric-Citations326LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Extraction,Ionization,Ions,Liquids,Solvents Get e-Alerts
MKK7 Is A Stress-activated Mitogen-activated Protein Kinase Kinase Functionally Related to hemipterous (1997)

Exposure of mammalian cells to stressful stimuli results in activation of the c-Jun NH2-terminal kinase (JNK)/stress-activated protein kinases (SAPKs), a family of protein kinases related to mitogen-activated protein (MAP) kinase. JNK/SAPKs are activated by specific MAP kinase kinases (MKKs), one of which, MKK4/SEK1, has been characterized extensively. In Drosophila, the JNK/SAPK Basket (Bsk) and the MKK Hemipterous (Hep), are important for embryonic development. Loss of function of either gene inhibits dorsal closure, a morphogenetic movement in which the edges of the embryonic ectoderm move together over the amnioserosa. There is evidence that the Rho GTPases Rac and Cdc42 are also required for dorsal closure, suggesting that Rac or Cdc42 may regulate Hep and Bsk. We have identified MKK7, a murine homolog of Hep. MKK7 functionally rescues hep mutant flies. In fibroblasts, MKK7 is activated by stress and by the GTPase Rac1. MKK7 directly phosphorylates and activates JNK/SAPK. Thus, MKK7 is a homolog of hep and functions in a conserved signaling pathway involving JNK/SAPK and the GTPase Rac1.
Correlations between visual fixation parameters, photosensitivity of the retina and visual acuity in various types of amblyopia (2021)

Microperimetry is an innovative tool for differential diagnosis, monitoring, method selection and evaluation of the effectiveness of treatment of various types of amblyopia.
Spatial vs temporal variability in German river water quality (2023)

River water quality is degraded by a multitude of diffuse and point sources impeding ecosystem functioning and constituting a severe risk for human water security all over the world. Monitoring campaigns are the basis of evaluating water quality by characterizing probability of concentrations in time and space, allowing to identify solute source zones and flow paths. This knowledge can then aid in the development of effective water quality management strategies. However, it is not clear, whether current monitoring approaches provide sufficient information to allow to soundly characterize concentration probability over time and localize pollution sources in space. We propose a space-time variance framework to characterize spatial and temporal variation in river water quality and analyze its interplay. Specifically, we assess for discharge and two contrasting solutes (anthropogenic: NO3-, biogenic: DOC) by analyzing time series data across 1386 stations in Germany (Ebeling et al. 2022) . Variability is quantified by using the Coefficient of Variation (CV) of mean temporal and spatial variation of subsets of catchments. We find a large span of both spatial and temporal CV for discharge, NO3- and DOC. Overall, variability of discharge was considerably higher in time and space than the variation of NO3- and DOC. Differences between CVs of NO3- and DOC were smaller than expected from their different landscape sources. Apart from analyzing national to continental-scale data records, we plan to analyze archetypal patterns of solutes by utilizing a stochastic modelling approach. Ultimately, the aim is to inform stakeholders whether monitoring strategies such as synoptic sampling are viable approaches and to disentangle anthropogenic and natural drivers to illuminate their role for spatial and temporal variation in river ecosystems.Ebeling, P., Kumar, R., Lutz, S. R., Nguyen, T., Sarrazin, F., Weber, M., B&amp;#252;ttner, O., Attinger, S., and Musolff, A.: QUADICA: water QUAlity, DIscharge and Catchment Attributes for large-sample studies in Germany, Earth Syst. Sci. Data, 14, 3715&amp;#8211;3741, https://doi.org/10.5194/essd-14-3715-2022, 2022.
Evolution of IgG Subclass Deficiency into Common Variable Immunodeficiency in a Child with Pulmonary Interstitial Glycogenosis (2008)

We present a 5-year-old boy who initially presented during infancy with pulmonary interstitial glycogenosis (PIG) and recurrent sinopulmonary infections. At 3.5 years old, IgG-2 subclass deficiency associated with low IgA level and selective antibody deficiency was diagnosed. Subsequently, the immune deficiency evolved into common variable immunodeficiency (CVID). This is the first case reported of the association of PIG and CVID. In children with PIG who have recurrent infections, associated immunodeficiency should be suspected.
Prepulse inhibition of the startle reflex in rats (1999)

Prepulse inhibition (PPI) of the startle reflex in rats is disrupted by N-methyl-D-aspartate (NMDA) receptor non-competitive antagonists (phencyclidine-like compounds). In order to explore more thoroughly the control exerted by NMDA receptors on PPI, we assessed the effects of i.p. administration, in Sprague-Dawley rats, of compounds acting as antagonists or agonists at the five binding sites of the NMDA receptor complex. The non-competitive NMDA receptor antagonists phencyclidine (1-6 mg/kg) and MK-801 (dizocilpine: 0.05-0.2 mg/kg) robustly and dose-dependently disrupted PPI. A similar effect was obtained with the competitive NMDA receptor antagonists CGS 19755 (1-20 mg/kg) and CPP (3-20 mg/kg), but not with the cation Mg2+ (100 and 200 mg/kg), the glycine/NMDA binding site antagonist L-701,324 (1-10 mg/kg), or the polyamine/NMDA binding site antagonist eliprodil (3-20 mg/kg). Potentiation of glutamatergic neurotransmission by NMDA (10-50 mg/kg), and the glycine/NMDA site partial agonist d-cycloserine (1-30 mg/kg) also failed to modify PPI, though d-cycloserine diminished PPI at higher doses (50-200 mg/kg). Co-administration of sub-threshold doses of CPP (3 mg/kg) and phencyclidine (2 mg/kg) resulted in an additive effect, disrupting PPI. In contrast, co-administration of L-701,324 (6 mg/kg) with phencyclidine (2 mg/kg), eliprodil (20 mg/kg), or CPP (3 mg/kg), did not disrupt PPI. These results demonstrate that PPI-disrupting effects can only be obtained with phencyclidine-like compounds and NMDA receptor competitive antagonists. Treatment with compounds that potentially augment glutamatergic tone were without effect. Finally, despite the permissive control of the glycine/NMDA binding site on glutamatergic neurotransmission, the glycine/NMDA binding site antagonist L-701,324 did not produce synergistic activity when combined with antagonists at the glutamate, polyamine/NMDA or phencyclidine-like compound binding sites.
Bias Contributions in a MEMS Tuning Fork Gyroscope (2012)

This paper analyzes the bias sources of mechanical and electrostatic origin in a tuning fork microelectromechanical systems (MEMS) gyroscope. In a gyroscope which is symmetrical and has no defect, there would be no bias; technological defects should be investigated in order to identify and quantify bias sources, which arise from quadrature and in-phase errors. Technological dispersions within the etching step of the mechanical structure are a major source of errors. Dispersions at the scale of a single MEMS particularly matter by impacting springs and electrodes. Design rules are defined for a <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">z</i> -axis gyroscope in order to curb these errors: In particular, specific drive springs, which are very stiff in the sense direction, and a mechanical decoupling between drive and sense are indicated to decrease errors. Local dispersion of the width of beams has been assessed with a network of electrical test structures and leads to a local standard deviation of 3-6 nm at the scale of a MEMS gyroscope device. The optimized design enables to obtain a total error of less than 300 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> /s on very compact <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">z</i> -axis gyroscopes with an area of mechanical part of 0.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
General Stress Transcription Factor ς B and Its Role in Acid Tolerance and Virulence of Listeria monocytogenes (1998)

The gene encoding the general stress transcription factor sigmaB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for sigmaB and the closely related sigmaF proteins grouped L. monocytogenes sigmaB in the same cluster with the sigmaB proteins from Bacillus subtilis and Staphylococcus aureus, (ii) the gene order in the 2, 668-bp portion of the L. monocytogenes sigB operon is rsbU-rsbV-rsbW-sigB-rsbX and is therefore identical to the order of the last five genes of the B. subtilis sigB operon, and (iii) an L. monocytogenes sigmaB mutant had reduced resistance to acid stress in comparison with its isogenic parent strain. The sigB mutant was further characterized in mouse models of listeriosis by determining recovery rates of the wild-type and mutant strains from livers and spleens following intragastric or intraperitoneal infection. Our results suggest that sigmaB-directed genes do not appear to be essential for the spread of L. monocytogenes to mouse liver or spleen at 2 and 4 days following intragastric or intraperitoneal infection.
Fiber-cavity-based optomechanical device (2012)

We describe an optomechanical device consisting of a fiber-based optical cavity containing a silicon nitride membrane. In comparison with typical free-space cavities, the fiber-cavity's small mode size (10 μm waist, 80 μm length) allows the use of smaller, lighter membranes and increases the cavity-membrane linear coupling to 3 GHz/nm and the quadratic coupling to 20 GHz/nm2. This device is also intrinsically fiber-coupled and uses glass ferrules for passive alignment. These improvements will greatly simplify the use of optomechanical systems, particularly in cryogenic settings. At room temperature, we expect these devices to be able to detect the shot noise of radiation pressure.
Central obesity is predictive of persistent storage lower urinary tract symptoms ( LUTS ) after surgery for benign prostatic enlargement: results of a multicentre prospective study (2015)

Increased WC is associated with persistent postoperative urinary symptoms after surgical treatment of BPE. Obese men have a higher risk of persistent storage LUTS after TURP or OP.
Combined GMSK and 8PSK modulator for GSM and EDGE (2003)

An efficient digital GMSK/8PSK I/Q signal modulator for GSM/EDGE is presented. The solution is based on Laurent's approximation of the non-linear GMSK modulator by a bank of linear, pre-encoded modulators, thus enabling burst shaping in the I/Q domain and therefore solving the problem of power ramping. The solution is compatible to all major transmitter architectures offering an I/Q interface.
Outgassing Characteristics of Stainless Steel and Aluminum with Different Surface Treatments (1969)

The outgassing characteristics of 304 stainless steel with different surface treatments have been measured. The surface treatments investigated were: (1) glass-bead shot blasting; (2) electropolishing with a surface roughness of 20–25 μ in.; (3) electropolishing with a surface roughness of 4–6 μ in.; (4) one of the above treatments plus oxidation by baking in air at 250 ° or 450 °C; (5) treatment (2) above plus 450 °C bake under vacuum. The outgassing characteristics of type-1100 aluminum, which had been cleaned with detergent and rinsed with acetone, were also determined. Results obtained indicate the outgassing rates for stainless steel cleaned by glass-bead shot blasting or electropolishing after a 250 °C bake for 15–30 h are approximately the same and equal to 2×10−12 Torr liter/sec cm2. These results are in agreement with the results reported by Calder and Lewin. Oxidized stainless steel produced a lower outgassing rate between 3 and 5×10−13 Torr liter/sec cm2. Also, a 450 °C bake for 17 h of stainless steel with the inner surface exposed to vacuum exhibited an outgassing rate of 4×10−13 Torr liter/sec cm2. Aluminum baked at 250 °C for 15 h provided an outgassing rate of 4×10−13 Torr liter/sec cm2. These results are explainable on the basis of H2 depletion from the samples due to high temperature baking for long periods of time and also the lower H2 permeation through oxide films.
Phosphorylation and Activation of p70 s6k by PDK1 (1998)

Activation of the protein p70s6k by mitogens leads to increased translation of a family of messenger RNAs that encode essential components of the protein synthetic apparatus. Activation of the kinase requires hierarchical phosphorylation at multiple sites, culminating in the phosphorylation of the threonine in position 229 (Thr229), in the catalytic domain. The homologous site in protein kinase B (PKB), Thr308, has been shown to be phosphorylated by the phosphoinositide-dependent protein kinase PDK1. A regulatory link between p70s6k and PKB was demonstrated, as PDK1 was found to selectively phosphorylate p70s6k at Thr229. More importantly, PDK1 activated p70s6k in vitro and in vivo, whereas the catalytically inactive PDK1 blocked insulin-induced activation of p70s6k.
The wild side of a major crop: Soybean's perennial cousins from Down Under (2014)

The accumulation of over 30 years of basic research on the biology, genetic variation, and evolution of the wild perennial relatives of soybean (Glycine max) provides a foundation to improve cultivated soybean. The cultivated soybean and its wild progenitor, G. soja, have a center of origin in eastern Asia and are the only two species in the annual subgenus Soja. Systematic and evolutionary studies of the ca. 30 perennial species of subgenus Glycine, native to Australia, have benefited from the availability of the G. max genomic sequence. The perennial species harbor many traits of interest to soybean breeders, among them resistance to major soybean pathogens such as cyst nematode and leaf rust. New species in the Australian subgenus continue to be described, due to the collection of new material and to insights gleaned through systematic studies of accessions in germplasm collections. Ongoing studies in perennial species focus on genomic regions that contain genes for key traits relevant to soybean breeding. These comparisons also include the homoeologous regions that are the result of polyploidy in the common ancestor of all Glycine species. Subgenus Glycine includes a complex of recently formed allopolyploids that are the focus of studies aimed at elucidating genomic, transcriptomic, physiological, taxonomic, morphological, developmental, and ecological processes related to polyploid evolution. Here we review what has been learned over the past 30 years and outline ongoing work on photosynthesis, nitrogen fixation, and floral biology, much of it drawing on new technologies and resources.
Shear‐bond strength and optical properties of short fiber‐reinforced CAD/CAM composite blocks (2021)

The aim of this study was to assess the shear-bond strength (SBS) of resin-luting cement to experimental short fiber-reinforced CAD/CAM composite (SFRC) compared to conventional CAD/CAM (Cerasmart 270), 3D printed (GC TEMP PRINT, Pro3dure GR-17), and laboratory (Gradia Plus) composites. Moreover, translucency parameter values and light transmission were evaluated. For each of the five types of composites, discs were prepared (n = 16/group) and divided into subgroups (n = 8/group) according to surface treatment protocol (hydrofluoric acid or air-particle abrasion). SBS test was performed using universal testing machine until failure, and failure modes were visually analyzed. Translucency parameter and curing-light transmission values through 1, 2, and 3 mm thickness were quantified using spectrophotometry and the MARC resin calibrator, respectively. Scanning electron microscopy (SEM) was used to examine the CAD/CAM composites after surface treatment. Composite type and surface treatment had a significant effect on SBS. Laboratory composite showed the highest SBS value (22.4 MPa). Cerasmart 270 exhibited higher translucency parameter values (19.8, 11.0, 5.0) than SFRC (14.5, 5.2, 1.6) at the three composite thicknesses tested. Air-particle abrasion was more effective in enhancing SBS than acid etching. Experimental SFRC CAD/CAM composite showed higher SBS values than Cerasmart 270. For all composites, translucency parameter values and light transmission decreased as thickness increased.
702. Detection and Isolation of Immune Cells After Intramuscular AAV Injection in Mouse and Human (2016)

Recombinant adeno-associated viral (rAAV) vectors are promising tools for gene transfer to the skeletal muscle. However, studies on mouse and large animal models as well as humans demonstrate that intramuscular (IM) rAAV delivery can trigger immune responses to AAV capsid and/or transgene. Recently, IM delivery of rAAV1 in humans induced a tolerogenic response to rAAV characterized by the presence of capsid-specific regulatory T cells (Tregs) in the periphery. The aim of our study was to develop an assay to allow detection and characterization of immune cells in situ after AAV gene transfer. Mice were injected intramuscularly with an AAV vector. Seven days after injection, muscles were collected and enzymatic dissociation was performed. Our results show that we are able to detect immune cells (monocytes/macrophages, lymphocytes) in mouse muscle by flow cytometry. This assay was also used on a human muscle biopsy collected 5 years after AAV1-AAT gene transfer and revealed the presence of infiltrated Tregs and CD8+ T-cells at the site of injection. This method appears as a new tool in understanding immune responses in the muscle after AAV gene transfer.
Protective Roles for RGS2 in a Mouse Model of House Dust Mite-Induced Airway Inflammation (2017)

The GTPase-accelerating protein, regulator of G-protein signalling 2 (RGS2) reduces signalling from G-protein-coupled receptors (GPCRs) that signal via Gαq. In humans, RGS2 expression is up-regulated by inhaled corticosteroids (ICSs) and long-acting β2-adrenoceptor agonists (LABAs) such that synergy is produced in combination. This may contribute to the superior clinical efficacy of ICS/LABA therapy in asthma relative to ICS alone. In a murine model of house dust mite (HDM)-induced airways inflammation, three weeks of intranasal HDM (25 μg, 3×/week) reduced lung function and induced granulocytic airways inflammation. Compared to wild type animals, Rgs2-/- mice showed airways hyperresponsiveness (increased airways resistance and reduced compliance). While HDM increased pulmonary inflammation observed on hematoxylin and eosin-stained sections, there was no difference between wild type and Rgs2-/- animals. HDM-induced mucus hypersecretion was also unaffected by RGS2 deficiency. However, inflammatory cell counts in the bronchoalveolar lavage fluid of Rgs2-/- animals were significantly increased (57%) compared to wild type animals and this correlated with increased granulocyte (neutrophil and eosinophil) numbers. Likewise, cytokine and chemokine (IL4, IL17, IL5, LIF, IL6, CSF3, CXCLl, CXCL10 and CXCL11) release was increased by HDM exposure. Compared to wild type, Rgs2-/- animals showed a trend towards increased expression for many cytokines/chemokines, with CCL3, CCL11, CXCL9 and CXCL10 being significantly enhanced. As RGS2 expression was unaffected by HDM exposure, these data indicate that RGS2 exerts tonic bronchoprotection in HDM-induced airways inflammation. Modest anti-inflammatory and anti-remodelling roles for RGS2 are also suggested. If translatable to humans, therapies that maximize RGS2 expression may prove advantageous.
Quantitative gait analysis following hemispherotomy for Rasmussen′s encephalitis (2007)

Peri-insular hemispherotomy is a form of disconnective hemispherectomy involving complete disconnection of all ascending / descending and commisural connections of one hemisphere. We report a case of a seven and a half year old child with intractable epilepsy due to Rasmussen's encephalitis who underwent peri-insular hemispherotomy and achieved complete freedom from seizures. Quantitative gait analysis was used to describe the changes in the kinematic and kinetic parameters of gait with surface electromyographs 18 months after surgery. The focus of this paper is to highlight the utility of gait analysis following hemispherotomy with a view to directing postsurgical motor training and rehabilitation.
Photorefractive keratectomy with a small spot laser and tracker. (1999)

Patients treated with the Autonomous Technologies LADARVision excimer laser system for -1.50 to -6.25 D of spherical myopia with 1 year follow-up had uncorrected visual acuity of 20/20 or better in 70%, no significant loss of spectacle-corrected visual acuity, no reduction of endothelial cell density or contrast sensitivity, and low levels of corneal haze.