gebhart/patrepeval huggingface dataset:
869 Anti-LunX targeting therapy for lung cancer (2020)

<h3>Background</h3> The identification of novel therapeutic targets in lung cancer for the generation of targeted drugs is an urgent challenge. Lung-specific X (LunX) is a member of the palate, lung, and nasal epithelium clone (PLUNC) protein family. Some reports have suggested that the human PLUNC gene (also named LUNX) might be a potential marker for NSCLC, and PLUNC mRNA has been identified in peripheral blood and mediastinal lymph nodes from NSCLC patients.It is unclear whether LunX expression is associated with the pathological type and pathological severity in lung cancer patients. The utility of LunX as a potential therapeutic target in NSCLC is uncertain. <h3>Methods</h3> Clinically, 80% of lung cancers are non-small-cell lung cancers (NSCLCs). Here, we analyzed 158 NSCLC samples and detected LunX expression. <h3>Results</h3> It showed that the expression of LunX were elevated in 90% (108/150) lung cancers by IHC staining, which accompanied with significantly lower rate of postsurgery survival. Further evaluation of LunX expression in invasive tumor cells in subclavicular lymph nodes, draining lymph nodes, hydrothorax of lung cancer patients, turned out that LunX is highly expressed in invasive lung cancer cells. These data indicated that LunX overexpresses in lung cancer and associates with tumorigenesis and tumor progression. Mechanistically, we discovered that LunX bound to 14-3-3 protein and facilitated their activation by maintaining these proteins in a dephosphorylated state, thereby contributing to the activation of pathways downstream of 14-3-3 protein, such as the Erk1/2 and JNK pathways. Thus, LunX promoted tumor growth and metastasis. Furthermore, we generated a therapeutic antibody specific for lung cancer, which not only inhibited lung cancer growth and reduced Ki67 staining and angiogenesis in xenograft model of subcutaneously transplanted tumor, but also blocked tumor metastasis and invasion, improved the survival of these mice. We also detected that antibody treatment induces LunX antigen-antibody complex endocytosis and the degradation of LunX protein. <h3>Conclusions</h3> Our study suggests that LunX is a novel therapeutic target in lung cancer and that the LunX-targeted therapeutic antibody may have considerable clinical benefit.
Piezoresistive and posistor effects in polymer-semiconductor and polymer-ferropiezoceramic composites (2016)

In this study, piezoresistive and posistor effects in polymer-semiconductor and polymer-ferropiezoceramic composites have been investigated. The results show that composites based on crystallizable polymers, such as PVDF, HDPE, and PP dispersed by semiconductors and ferropiezoelectric fillers have piezoresistive and posistor properties, respectively. At low pressure, charge carriers tunneling through the located thin polymer among filler particles into the barrier define the conductivity of the composite. When pressure value is increased from 0 to 1 MPa, the thickness of the interlayer decreases and tunnel conductivity descends exponentially depending on barrier height. The piezoresistor sensitivity of a composite based on PVDF-70 vol % + Si-30 vol % is higher than a composite based on HDPE-70 vol % + Ge-30 vol %. Furthermore, the posistor properties of polymer composites dispersed by ferropiezoceramic are determined as the maximum resistance that varies significantly with temperature. Posistor effect in composites based on polymer + ferropiezoceramic is associated with the height of the barrier layer, which changes according to properties of filler, polymer, and dielectric permittivity of two-phase composites. The highest specific resistance related to HDPE-70 vol % + BaTiO3-30 vol % composite was observed at ~403 K.
Btn2a2 Regulates ILC2–T Cell Cross Talk in Type 2 Immune Responses (2022)

Innate lymphoid cells (ILC) not only are responsible for shaping the innate immune response but also actively modulate T cell responses. However, the molecular processes regulating ILC-T cell interaction are not yet completely understood. The protein butyrophilin 2a2 (Btn2a2), a co-stimulatory molecule first identified on antigen-presenting cells, has a pivotal role in the maintenance of T cell homeostasis, but the main effector cell and the respective ligands remain elusive. We analyzed the role of Btn2a2 in the ILC-T cell cross talk. We found that the expression of Btn2a2 is upregulated in ILC2 following stimulation with IL-33/IL-25/TSLP. <i>In vitro</i> and <i>in vivo</i> experiments indicated that lack of Btn2a2 expression on ILC2 resulted in elevated T cell responses. We observed an enhanced proliferation of T cells as well as increased secretion of the type 2 cytokines IL-4/IL-5/IL-13 following cocultures with Btn2a2-deficient ILC2. <i>In vivo</i> transfer experiments confirmed the regulatory role of Btn2a2 on ILC2 as Btn2a2-deficient ILC2 induced stronger T cell responses and prevented chronic helminth infections. Taken together, we identified Btn2a2 as a significant player in the regulation of ILC2-T cell interactions.
The Grb2 adaptor (1995)

Grb2 is an 'adaptor' protein made of one SH2 and two SH3 domains. The SH3 domains bind to prolinerich motifs in the C-terminal part of the ras exchange factor Sos. Binding of the Grb2 SH2 domain to phosphotyrosine motifs on receptors, or other adaptor proteins such as Shc, recruits this Grb2/Sos complex at the plasma membrane where Sos stimulates nucleotide exchange on ras, then ras activates raf and leads to MAP kinase activation. The structure of Grb2, the precise motifs recognised by its SH2 and SH3 domains, the way Grb2 performs its function, a possible regulation of its association with Sos, and its ability to complex with other proteins in vivo, are discussed.
The components of bid‐ask spread and their determinants: TAIFEX versus SGX‐DT (2004)

Abstract This paper compares the intraday components of bid‐ask spread in Taiwan stock index futures traded on Taiwan Futures Exchange (TAIFEX) and Singapore Exchange Derivatives Trading Limited (SGX‐DT). Variables that determine the components of spread are also examined. SGX‐DT uses a floor trading system while TAIFEX uses an electronic call system. This study finds that both information asymmetry and order processing cost components exhibit U‐shaped patterns in the two markets, in contrast to previous findings for U.S. equity markets. Moreover, the information asymmetry components are lower in the TAIFEX relative to the SGX‐DT futures, suggesting that the continuous open outcry markets are more vulnerable to information asymmetry than the electronic call markets. The regression results show that volatility and information are the major determinants of the components while number of trades is not the major determinant of the order processing and information asymmetry components for both markets. © 2004 Wiley Periodicals, Inc. Jrl Fut Mark 24:835–860, 2004
Nanosized Delivery Systems for Tissue Regeneration (2016)

This chapter focuses on advanced delivery of biologics including growth factors (GFs), cytokines, genes, or siRNAs using a variety of nanosized systems for different regeneration applications focusing on bone, cartilage, nervous system, and muscle regeneration strategies. Gene therapy provides permanent genetic alteration, but only transient actions of protein therapeutics can be needed for tissue regeneration applications. The limitations of biologics delivery and alternative strategies for overcoming recent problems are underlined presenting recent examples from the literature. In addition, specific targeting and cellular internalization strategies of biologies delivery for tissue regeneration are discussed for providing future perspectives to the readers in this field. Overall, it is believed that advanced nanosized delivery systems integrated with multicomponent designs will open new opportunities in delivery technologies and strategies for tissue regeneration.
Circadian and ultradian blood pressure variability assessed by periodic regression (1992)

Automatic hardware for time-specified sampling is here combined with a set of software modules for signal processing by periodic regression in order to assess the extent of blood pressure variability within a day in ordinary conditions. The predictable variability thus identified can be used in screening and prevention as well as for diagnosis and cure. <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup>
Robotic Radionuclide Inspection and Mapping of Surface Contamination On Building Surfaces (2007)

The mapping of localized regions of radionuclide contamination in a building can be a time consuming and costly task. Humans moving hand-held radiation detectors over the target areas are subject to fatigue. A contamination map based on manual surveys can contain significant operator-induced inaccuracies. A Fanuc M16i light industrial robot has been configured for installation on a mobile aerial work platform, such as a tall forklift. When positioned in front of a surface, the robot can map the radiation levels over a surface area of up to 2 m by 2 m. The robot's end effector is a commercial alpha-beta radiation sensor, augmented with range and collision avoidance sensors to ensure operational safety as well as to maintain a constant gap between surface and radiation sensors. A graphical user interface guides the robot operator to position the robot at the desired wall segments, and to select an area for surveying. After the operator has entered the required parameters, the custom surveying software plans the scan sequence, alerts of any potential problems, such as unreachable singularities, and creates a contamination map of the surveyed region. Maps of multiple regions can be combined into a single map of the entire region. Themore » survey data are stored in a data base file. In addition to automated surface scans, operators can manually select regions for further inspection, as well as control the end effector motion manually. In comparison to manual contamination surveys, the robotic approach is more accurate, reliable, and faster. (authors)« less
Axial Changes of Blood and Plasma Flow, Pressure, and Cellular Deposition in Capillary Plasma Filters (1981)

The ability to separate plasma on-line from whole blood by membranes has generated interest in applying this process to source plasma collection and to the therapeutic treatment of various disease states. The availability of capillary membrane devices in particular has prompted investigations in these areas. The deposition of blood cellular elements has required the investigation of those parameters related to fluid and mass flux. Analytical solutions relating blood flow rate, filtration rate, and pressure and deposition with respect to axial distance have been obtained. Comparison of these solutions with preliminary experimental results have been made. Results indicate that the deposition parameter versus distance from the inlet is a very sensitive function of capillary membrane properties and operating conditions. Plasma fluxes are also highly dependent on blood, membrane, and filter properties and operating conditions.
Development of Mupirocin Resistance among Methicillin-Resistant Staphylococcus aureus after Widespread Use of Nasal Mupirocin Ointment (1996)

Mark A. Miller, Andre Dascal, Joseph Portnoy, Jack Mendelson, Development of Mupirocin Resistance among Methicillin-Resistant Staphylococcus aureus after Widespread Use of Nasal Mupirocin Ointment, Infection Control and Hospital Epidemiology, Vol. 17, No. 12 (Dec., 1996), pp. 811-813
Fusion of global and local information for object detection (2003)

This paper presents a framework for fusing together global and local information in images to form a powerful object detection system. We begin by describing two detection algorithms. The first algorithm uses independent component analysis to derive an image representation that captures global information in the input data. The second algorithm uses a part-based representation that relies on local properties of the data. The strengths of the two detection algorithms are then combined to form a more powerful detector The approach is evaluated on a database of real-world images containing side views of cars. The combined detector gives distinctly superior performance than each of the individual detectors, achieving a high detection accuracy of 94% on this difficult test set.
DETECTING OBSTACLES FOR AN UNMANNED GROUND VEHICLE USING POLARIZATION VISION (1998)

This paper describes a new polarization sensor technology which cam be used for obstacle detection for unmanned autonomous and semi- autonomous vehicles. The method involves measuring the polarization parameters of light augmenting the information acquired by existing intensity video sensors. Algorithms that are based upon the physics of reflected polarization of lights yield distinctive physical features of terrain which an unmanned vehicle can then use to avoid obstacles.
Persistence of the Antibody Response to the VlsE Sixth Invariant Region (IR6) Peptide ofBorrelia burgdorferiafter Successful Antibiotic Treatment of Lyme Disease (2003)

It has been suggested that a <4-fold decline in the immunoglobulin G (IgG) antibody response to the VlsE sixth invariant region peptide of Borrelia burgdorferi within 6 months after antibiotic treatment may indicate spirochetal persistence in Lyme disease. We studied the response to this peptide in 77 patients with early or late disease, for whom archival samples were available at the time of antibiotic treatment and approximately 6 months or years later. Eight (33%) of the 24 patients with early manifestations and 18 (86%) of the 21 patients with late manifestations had a <4-fold decline in IgG anti-VlsE titers approximately 6 months after successful antibiotic treatment. Of 32 additional patients, 13 (50%) with early manifestations and 5 (83%) with late manifestations still had positive anti-VlsE titers 8-15 years after successful antibiotic treatment. We conclude that persistence of the anti-VlsE antibody response for months or years after antibiotic treatment cannot be equated with spirochetal persistence in Lyme disease.
Single-frequency synthesis at telecommunication wavelengths (2009)

We report an optical single-frequency synthesizer at the 1.55 microm telecommunications band. Output from a continuous-wave external cavity diode laser is frequency doubled and phase locked to a predetermined component of a Ti:S laser frequency comb. The synthesizer is capable of generating a single user-specified frequency from an atomic time base within the 192-196 THz gain bandwidth of an erbium-doped fiber amplifier. By tuning the repetition rate of the femtosecond laser the synthesized optical frequency can be swept with sub-kilohertz step size. Frequency sweeps of several GHz are realized by automatically re-locking the diode laser to adjacent comb components during frequency sweep. We demonstrate the operation of the device by presenting results of Doppler-free spectroscopy on acetylene using synthesized frequencies.
New designer drug N‐(1‐phenylcyclohexyl)‐3‐ethoxypropanamine (PCEPA): Studies on its metabolism and toxicological detection in rat urine using gas chromatographic/mass spectrometric techniques (2006)

Studies are described on the metabolism and toxicological detection of the phencyclidine-derived designer drug N-(1-phenylcyclohexyl)-3-ethoxypropanamine (PCEPA) in rat urine using gas chromatographic/mass spectrometric techniques. The identified metabolites indicated that PCEPA was metabolized by N-dealkylation, O-deethylation partially followed by oxidation of the resulting alcohol to the corresponding carboxylic acid, hydroxylation of the cyclohexyl ring at different positions of PCEPA, N-dealkyl PCEPA, O-deethyl PCEPA, and of the corresponding carboxylic acids. Finally, aromatic hydroxylation of PCEPA, the corresponding carboxylic acids, and O-deethyl PCEPA, the latter partially followed by oxidation to the corresponding carboxylic acid and hydroxylation of the cyclohexyl ring could be observed. All metabolites were partially excreted in the conjugated form. The authors' systematic toxicological analysis (STA) procedure using full-scan GC/MS after acid hydrolysis, liquid-liquid extraction, and microwave-assisted acetylation allowed the detection in rat urine of an intake of a common drug users' dose of PCEPA. Assuming a similar metabolism in humans, the STA in human urine should be suitable as proof of intake of PCEPA.
Fluorescent Polymer Nanoparticles for Cell Barcoding In Vitro and In Vivo (2017)

Fluorescent polymer nanoparticles for long-term labeling and tracking of living cells with any desired color code are developed. They are built from biodegradable poly(lactic-co-glycolic acid) polymer loaded with cyanine dyes (DiO, DiI, and DiD) with the help of bulky fluorinated counterions, which minimize aggregation-caused quenching. At the single particle level, these particles are ≈20-fold brighter than quantum dots of similar color. Due to their identical 40 nm size and surface properties, these nanoparticles are endocytosed equally well by living cells. Mixing nanoparticles of three colors in different proportions generates a homogeneous RGB (red, green, and blue) barcode in cells, which is transmitted through many cell generations. Cell barcoding is validated on 7 cell lines (HeLa, KB, embryonic kidney (293T), Chinese hamster ovary, rat basophilic leucemia, U97, and D2A1), 13 color codes, and it enables simultaneous tracking of co-cultured barcoded cell populations for >2 weeks. It is also applied to studying competition among drug-treated cell populations. This technology enabled six-color imaging in vivo for (1) tracking xenografted cancer cells and (2) monitoring morphogenesis after microinjection in zebrafish embryos. In addition to a robust method of multicolor cell labeling and tracking, this work suggests that multiple functions can be co-localized inside cells by combining structurally close nanoparticles carrying different functions.
The 68040 processor. 2. Memory design and chip (1990)

For pt.1 see ibid., February (1990). The memory subsystem, the external bus, chip and board testing, and design-verification methods for the 68040, a third-generation, full-32-bit microprocessor in the Motorola 68000 family, are discussed. The internal caches and memory management are examined at length. The external bus protocol, arbitration, snooping, and timing specifications are addressed. The MOVE16 instruction, which moves a cache line from one address (which may reside in the data cache) to another address outside the cache is described. User testing, based on dedicated test logic that is fully compliant with the IEEE 1149.1 standard, and factory testing, for which the processor employs structured design techniques for random logic and special test modes for embedded arrays, are examined. The use of top-down design and a hierarchical method of design verification is discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Genome Scan for Therapy-Related Leukemia. (2005)

Abstract Therapy-related leukemia (t-ML) is a rare but devastating complication of anticancer treatment, including treatment of acute lymphoblastic leukemia (ALL). Although a number of treatment factors have been identified that contribute to the development of t-ML, little is known about the biology of the disease or the genetic risk factors. To identify possible genetic risk factors for and characteristics of t-ML among ALL patients, we used the Affymetrix GeneChip® Human Mapping 100K set to genotype over 100,000 single nucleotide polymorphisms (SNPs) in germline DNA from 13 cases with t-ML and from 13 matched controls, as well as DNA of the t-ML blast samples of the 13 cases. Controls were matched for treatment protocol, ALL risk classification, irradiation, prior use of G-CSF, and race. Germline allele frequencies differed (at the p &amp;lt; 0.01 level) for 309 SNPs (203 genes) between t-ML cases and controls. Cytogenetically, most of the cases had translocations or inversions of 11q23; however, three cytogenetically-detected regions of chromosomal loss were also detected (on chromosome 5, 7, and 9, each found only once among the cases). These chromosomal deletions were all detected as regions of loss of heterozygosity (LOH) and decreased copy number by paired intra-patient germline vs t-ML blast analyses of SNP calls. However, the paired SNP data indicated 216 regions of LOH (FDR = 2.5%) corresponding to a total of 81 non-contiguous regions (Figure), most of which were not detected as losses cytogenetically, and 38 shared (among pairs) regions of LOH. As many as 45 noncontiguous and 12 contiguous LOH regions were found among those cases with no cytogenetically defined regions of loss, including patients with simple balanced translocations. In addition to cytogenetically-defined cases, using SNP analyses we detected additional t-ML cases with regions of LOH on 5q (n=1), on chromosome 7 (n=4), and on chromosome 9 (n=2). In fact, only one patient, whose cytogenetics showed a balanced translocation involving 11q23, had no extended regions of LOH detected by SNP analysis. Altogether, 5 of the 13 cases demonstrated LOH on chromosome 7. Using a change-point model, we detected 18 regions of copy number increase and 21 regions of copy number decrease in the blasts compared to paired germline DNA (p&amp;lt;0.01). We conclude that dense genotyping of SNPs not only accurately detects cytogenetically defined regions of gain or loss, but also chromosome abnormalities not found by conventional techniques. Genes associated with SNPs whose frequencies differed between cases and controls, with LOH, or altered copy number, are involved in apoptosis, DNA damage repair, and novel pathways, thereby providing candidate genes that may contribute to the development of t-ML. Figure: 81 regions of inferred LOH in case t-ML blast DNA compared to germline DNA indicated losses involving multiple autosomes. Figure:. 81 regions of inferred LOH in case t-ML blast DNA compared to germline DNA indicated losses involving multiple autosomes.
Antioxidants decrease cell death in experimentally wounded articular cartilage (2002)

Conference Abstract| October 01 2002 Antioxidants decrease cell death in experimentally wounded articular cartilage I.M. Khan; I.M. Khan 1Cardiff School of Biosciences, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff, CF10 3US, UK Search for other works by this author on: This Site PubMed Google Scholar B. Thomson; B. Thomson 1Cardiff School of Biosciences, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff, CF10 3US, UK Search for other works by this author on: This Site PubMed Google Scholar C.W. Archer C.W. Archer 1Cardiff School of Biosciences, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff, CF10 3US, UK Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 2002 Biochemical Society2002 Biochem Soc Trans (2002) 30 (5): A127. https://doi.org/10.1042/bst030a127a Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation I.M. Khan, B. Thomson, C.W. Archer; Antioxidants decrease cell death in experimentally wounded articular cartilage. Biochem Soc Trans 1 October 2002; 30 (5): A127. doi: https://doi.org/10.1042/bst030a127a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 2002 Biochemical Society2002 Article PDF first page preview Close Modal You do not currently have access to this content.