gebhart/patrepeval huggingface dataset:
Effect of Methylisobutyl Amiloride on [Na+]i, Reperfusion Arrhythmias, and Function in Ischemic Rat Hearts (1996)

With 2 microM methylisobutyl amiloride (MIA), an inhibitor of Na+/H+ exchange, we tested the hypothesis that ion imbalance due to H+/Na+/Ca2+ exchange exacerbates reperfusion injury and arrhythmias. Isolated rat hearts were subjected to 25-min global ischemia and 30-min reperfusion. In the MIA-treated group, MIA was added throughout the perfusion protocol. Left ventricular pressure (LVP), arrhythmias, myocardial Na+ and K+ content, 45Ca2+ uptake, and the levels of energy metabolites were analyzed. The recovery of LV developed pressure (LVDP) and +dP/dt and -dP/dt were improved in the MIA group (53 vs. 80, 71 vs. 86, 77 vs. 94%: each p < 0.05). MIA inhibited the increase in Na+ content and the decrease in K+ content that occurred at the end of the ischemic phase and reduced 45Ca2+ uptake after reperfusion (28.6 vs. 17.1, 248 vs. 296, 2.79 vs. 1.36 microM/g dry weight of tissue; each p < 0.05). The incidence of ventricular tachycardia (VT) or ventricular fibrillation (VF) was lower in the MIA group [VT 11 of 20 (55%) vs. 4 of 20 (20%), p < 0.05; VF 13 of 20, (65%) vs. 6 of 20 (30%), 0.05 < p < 0.1], although the incidence of VF just escaped statistical significance. ATP level was higher in the MIA group after the ischemic phase and reperfusion (5.3 vs. 9.9, 12.3 vs. 14.7 microM/g dry weight of tissue; each p < 0.05). Our results suggest that MIA reduced reperfusion arrhythmias and improved functional recovery in isolated rat hearts subjected to global ischemia apparently by preserving high-energy phosphates during ischemia and by inhibiting Na+/H+ exchange, with attenuated cellular imbalance between Na+ and Ca2+.
Rationally Engineered Mutants of Phosphotriesterase for Preparative Scale Isolation of Chiral Organophosphates (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTRationally Engineered Mutants of Phosphotriesterase for Preparative Scale Isolation of Chiral OrganophosphatesFeiyue Wu, Wen-Shan Li, Misty Chen-Goodspeed, Miguel A. Sogorb, and Frank M. RaushelView Author Information Department of Chemistry, Texas A&M University P.O. Box 30012, College Station, Texas 77842-3012 Cite this: J. Am. Chem. Soc. 2000, 122, 41, 10206–10207Publication Date (Web):September 28, 2000Publication History Received17 July 2000Published online28 September 2000Published inissue 1 October 2000https://pubs.acs.org/doi/10.1021/ja002546rhttps://doi.org/10.1021/ja002546rrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views355Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Hydrolysis,Mixtures,Organophosphorus compounds,Peptides and proteins,Vinyl Get e-Alerts
Frequency division multiplexed 1 Gsymbol/s, 64 QAM coherent optical transmission with a spectral efficiency of 8.6 bit/s/Hz (2008)

We have successfully transmitted a three-channel frequency division multiplexed 1-Gsymbol/s, 64 QAM coherent optical signal over 160-km with a 1.4-GHz spacing. The spectral efficiency reaches as high as 8.6-bit/s/Hz.
Low-grade systemic inflammation and the development of type 2 diabetes mellitus [ndash] : the ARIC Study (2003)

Results:A low grade, systemic inflammation precedes and predicts type 2 diabetes.The purpose of this study is to examine this association according to various markers of inflammation as well as to characterize its heterogeneity across specific subgroups.We designed in a case-cohort study representing the approximately 9 year experience of 10275 eligible Atherosclerosis Risk in Communities Study participants.Analytes were measured on stored plasma on 581 incident cases of diabetes and 572 non-cases.We constructed an overall inflammation score, ranging from 0 to 6, based on the presence of above-median values for interleukin-6, C-reactive protein, orosomucoid, sialic acid, white cell count and fibrinogen.Score values were higher in smokers and in the overweight.We estimated the score[rsquo]s association with incident diabetes utilizing proportional hazards models adjusted for age, parental history of diabetes, hypertension, body mass index (BMI), waist-hip ratio, and fasting glucose and insulin.Higher score values predicted diabetes among whites but not among African-Americans (interaction P<0.001), and among non-smokers but not among smokers (interaction P=0.03).For white non-smokers, hazard ratios were 1.0, 1.7, 2.4, 3.9, 2.1 and 3.9 for those with 1, 2, 3, 4, 5 and 6 inflammation markers above the median, respectively, in comparison to those with no markers with above-median values (P=0.03 for linear trend).The hazard ratio for each unit increment in the inflammation score was 1.0 (0.74 [ndash] 1.4) for those with BMI < 25 kg/m[sup2], 1.3 (1.1 [ndash] 1.6) for those with BMI between 25 and 30 kg/m[sup2], and 1.5 (0.98 [ndash] 2.4) for those with BMI [ge] 30 kg/m[sup2] (inflammation score [ndash] BMI interaction P=0.03).In conclusion, a low-grade inflammation precedes and predicts the development of type 2 diabetes.The association is not present in smokers, African-Americans and lean individuals.
Faraday Rotation of Rare Earth Ions in Orthoferrites (1980)

The Faraday rotation spectra corresponding to the 4 I 15/2 → 4 F 9/2 (Er 3+ ) and 3 H 6 → 3 F 2 (Tm 3+ ) transitions in ErFeO 3 and TmFeO 3 were found to be in dissipative and dispersive shapes, respectively, in both the \(\varGamma_{2}(F_{x})\) and \(\varGamma_{4}(F_{z})\) spin configurations. The Faraday rotation due to the electronic transition of the rare earth ions (R 3+ ) was analysed in terms of the molecular field perturbation acting on the R 3+ ions. In the case of the R 3+ ions with odd number of electrons such as Er 3+ (4 f 11 ), the crystal field state of the J -multiplet is split into degenerate Kramers doublets which makes the spectrum of the Faraday rotation dissipative when \(\varGamma(\text{linewidth}){\lesssim}\varDelta(\text{Kramers splitting}){\ll}\text{k}T\); while in the case of the R 3+ ions with even number of electrons such as Tm 3+ (4 f 12 ), the crystal field state is split into nondegenerate singlets, which makes the spectrum of the Faraday rotation dispersive.
Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing (2018)

A biodegradable two-dimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3-BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradation-associated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3-BPs thus provide efficient genome editing and gene silencing in vitro and in vivo at a relatively low dose as compared with other nanoparticle-based delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications.
Formation of Hierarchical Hybrid Silica-Polymer Using Quantitative Cryo- Electron Tomography (2019)

Journal Article Formation of Hierarchical Hybrid Silica-Polymer Using Quantitative Cryo- Electron Tomography Get access M-A Moradi, M-A Moradi Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar D Eren, D Eren Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar S Rzadkievics, S Rzadkievics Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar A Keizer, A Keizer Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar M M J van Rijt, M M J van Rijt Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar P H H Bomans, P H H Bomans Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar H Friedrich, H Friedrich Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar N A J M Sommerdijk, N A J M Sommerdijk Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar J P Patterson J P Patterson Laboratory of Materials and Interface Chemistry and Center for Multiscale Electron Microscopy, Eindhoven University of Technology, Den Dolech 2, 5600 MB Eindhoven, The Netherlands Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 25, Issue S1, 1 February 2019, Pages 59–60, https://doi.org/10.1017/S1431927618016021 Published: 01 February 2019
Cloning and transcriptional expression of a leucokinin‐like peptide receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae) (2000)

Leucokinins are invertebrate neuropeptides that exhibit myotropic and diuretic activity. Only one leucokinin-like peptide receptor is known, the lymnokinin receptor from the mollusc Lymnaea stagnalis. A cDNA encoding a leucokinin-like peptide receptor was cloned from the Southern cattle tick, Boophilus microplus, a pest of cattle world-wide. This is the first neuropeptide receptor known from the Acari and the second known in the subfamily of leucokinin-like peptide G-protein-coupled receptors. The deduced amino acid sequence exhibits 40% identity to the lymnokinin receptor. The receptor transcript is present in all tick life stages as determined by semiquantitative reverse transcription polymerase chain reaction. We also propose that the sequence AAF50775.1 from the Drosophila melanogaster genome (CG10626) encodes the first identified insect leucokinin receptor.
Indefinite matrices of linear electrical circuits, their pseudoinverses, and applications in related fields (2010)

This paper presents the group of indefinite matrices of linear circuits in a general context. Specifically, the indefinite modified admittance matrix and its pseudo-inverse are shown for the first time. Closed formulas for their respective pseudo-inverses, and further examples from both electrical and nonelectrical fields are also shown.
Adequacy of dialysis. (1988)

(1) There is no single measurement of adequacy in dialysis available now, and there likely never will be. (2) With present knowledge, it is rational to model patients for removal of both small and middle molecules. Minimum weekly clearances for urea and for a middle molecule the size of Vitamin B12 should be 120 and 30 liters per week per 1.73 m2 respectively. (3) In prescribing a dialysis treatment, a Kt/v urea greater than 1 should be targeted, a value of under 0.8 is unacceptable. (4) Attention to the nutritional status of the patient is important, and one should strive for a PCR of 1.1 to 1.3 g/kg/day. (5) Studies on living cells (for example, platelets) and systems (for example, CNS function) are valuable if there is doubt as to the patient's status. (6) Assessments of treatment stresses and quality of life should be used in monitoring dialysis patients. (7) In clinical trials of different treatment methods, it is important to equate findings to solute clearance profiles and to include studies on functions of cells or systems and to measure the impact of the treatment upon quality of life.
Floc Formation of Ethanol Producing <i>Escherichia coli</i> KO11 Cells and Its Application to Repeated Batch Operation (2016)

Microbial flocculation is an aggregation phenomenon of bacterial cells in the form of flocs or flakes. In this study, we endowed ethanol producing E. coli KO11 cells with floc-forming ability by overexpressing a native bcsB gene (KO11/pNbcsB) without negative effect on ethanol production. Quantitative evaluation of the formed flocs was conducted on the basis of the protein amount included within floc structure. The results showed that glucose concentration and culture temperature were important parameters for E. coli floc formation. In particular, a temperature shift from 37 to 30°C suppressed the floc formation at a negligible level and the shift to 40°C doubled the amount of flocs. The sedimentation test confirmed that E. coli flocs completed sedimentation within 15 min after cessation of shaking while planktonic cells kept suspending. Finally, the repeated batch operation was performed to demonstrate the advantage of flocculating E. coli KO11. The amount of deposited cells in the culture of KO11/pNbcsB strain was about two times greater than that of original KO11 strain, thereby leading to a significant increase of ethanol productivity. In addition, the flocs lasted to be produced during the repeated batch operation, suggesting that the productivity will be enhanced in every operation.
Kinetics of dissociation of thorium(IV) bound to polymaleic acid and poly(methylvinylether) maleic acid (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetics of dissociation of thorium(IV) bound to polymaleic acid and poly(methylvinylether) maleic acidG. R. Choppin and W. CacherisCite this: Inorg. Chem. 1990, 29, 7, 1370–1374Publication Date (Print):April 1, 1990Publication History Published online1 May 2002Published inissue 1 April 1990https://pubs.acs.org/doi/10.1021/ic00332a017https://doi.org/10.1021/ic00332a017research-articleACS PublicationsRequest reuse permissionsArticle Views40Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Preliminary study of calibration-free continuous glucose monitoring with microdialysis technique (2002)

To develop an artificial pancreas, measurement of subcutaneous glucose concentration by using a microdialysis technique is required. However, long-term continuous measurement has not been achieved because the microdialysis probe recovery rate and the sensitivity of the glucose sensor decrease. To solve this problem, a new method for long-term continuous measurement of subcutaneous tissue glucose concentration using a microdialysis technique was developed. We assumed a model of standard glucose solutions of two different concentrations, which are perfused via a microdialysis probe inserted subcutaneously. The standard solutions are switched by changing the valve. One solution is higher in concentration than the tissue and the other is lower. By controlling the perfusion time ratio of the standard solutions, we will automatically be able to calculate the glucose concentration of the tissue from the ratio of perfusion time when the glucose sensor signal at the microdialysis probe outlet is steady. In our results, measurement error was less than 10% in the experiment using a larger-scaled hollow fiber instead of a microdialysis probe. This study suggests a method for continuous measuring of subcutaneous tissue glucose concentration without calibration.
Supplementary Figure S3 from Targeting Fibroblast Growth Factor Receptor 1 for Treatment of Soft-Tissue Sarcoma (2023)

&lt;p&gt;Recurrent amplification and overexpression of FRS2 in STS.&lt;/p&gt;
Association of neutrophil extracellular traps with the production of circulating DNA in patients with colorectal cancer (2022)

We postulate that a significant part of circulating DNA (cirDNA) originates in the degradation of neutrophil extracellular traps (NETs). In this study, we examined the plasma level of two markers of NETs (myeloperoxidase (MPO) and neutrophil elastase (NE)), as well as cirDNA levels in 219 patients with a metastatic colorectal cancer (mCRC), and in 114 healthy individuals (HI). We found that in patients with mCRC the content of these analytes was (i) highly correlated, and (ii) all statistically different (<i>p</i> < 0.0001) than in HI (N = 114). These three NETs markers may readily distinguish between patients with mCRC from HI, (0.88, 0.86, 0.84, and 0.95 AUC values for NE, MPO, cirDNA, and NE + MPO + cirDNA, respectively). Concomitant analysis of anti-phospholipid (anti-cardiolipin), NE, MPO, and cirDNA plasma concentrations in patients with mCRC might have value for thrombosis prevention, and suggested that NETosis may be a critical factor in the immunological response/phenomena linked to tumor progression.
Colonization Rate, Serotypes, and Distributions of Macrolide-Lincosamide-StreptograminBResistant Types of Group B Streptococci in Pregnant Women (2009)

The prevalence of neonatal group B streptococcal infection depends mainly on the colonization rate of pregnant women by group B streptococci (GBS). Although the colonization rate of Korean women by GBS is considered lower than in other countries, recent data on the maternal colonization rate of GBS are sparse. Methods: From August 2008 to June 2009, swab specimens from the anorectus, vagina, and urethral orifice of a sample of 234 pregnant Korean women were placed in new Granada medium (NGM-H), tube medium (NGM-T), commercial NGM (NGM-B), and selective Todd-Hewitt broth (S-THB) for 1824 hours in 5% CO2 at 35 o C. Agar dilutional antimicrobial susceptibility tests, serotyping, and PCR were performed for GBS isolates.
Joint Design on DCN Placement and Survivable Cloud Service Provision over All-Optical Mesh Networks (2014)

Cloud services based on data center networks (DCNs) require a transmission infrastructure with high-capacity, low-latency, low-cost and high-availability, which can be offered by survivable optical networks. DCN placement is a fundamental issue in supporting cloud services in optical networks. It concerns not only the cost of providing cloud services, but also the service availability against failures via proper service replicas. In this paper, we jointly optimize DCN placement with service routing and protection to minimize the network cost, while ensuring fast protection of all services against any single link failure or service failure at a particular DCN. An ILP (Integer Linear Program) is first formulated to achieve optimal joint design. It integrates p-cycle (preconfigured protection cycle) for fast protection against a single link failure, and DCN replicas and fast service rerouting against a service failure. To make the design more scalable, a two-step heuristic is then proposed for large-size network scenarios. The first step separately solves the DCN placement and service routing problem in the failure-free scenario, and the second step takes fast service protection into account. The proposed design is validated by extensive numerical experiments.
Application and analysis of structural acoustic optimization techniques in smart structure product development and systems engineering (2010)

10 S.
Exposure of Bronchial Epithelial Cells to Whole Cigarette Smoke: Assessment of Cellular Responses (2005)

Cigarette smoke is composed of approximately 5% particulate phase and 95% vapour phase by weight. However, routine in vitro toxicological testing of smoke normally only measures the activity of the particulate phase. This study describes a new system for exposing cells at an air-liquid interface to serial dilutions of gaseous smoke. Confluent monolayers of NCI-H292 human lung epithelial cells on semipermeable membranes were placed in a purpose-designed Perspex chamber at an air-liquid interface. The cells were exposed to dilute whole mainstream cigarette smoke for 30 minutes, followed by a 20-hour recovery period. Firstly, high and low delivery cigarettes were compared, and cytotoxicity was determined by using the neutral red uptake assay. Clear differential cytotoxic responses were observed with the two cigarette types, which correlated positively with the concentrations of components in smoke, and particularly compounds in the vapour phase, such as aldehydes. Secondly, low doses of smoke were found to up-regulate mRNA levels of the secreted mucin, MUC5AC, and to stimulate the production of interleukin (IL)-6, IL-8 and matrix-metalloprotease-1, but had no effect on growth-related oncogene alpha. This system will facilitate further investigations into the toxicological mechanisms of cigarette smoke components, and may be useful for studying other gaseous mixtures or aerosols.
“Frustrated Lewis Pairs”: A Metal‐Free Strategy for Hydrogenation Catalysis (2010)

This chapter contains sections titled: Phosphine-Borane Activation of H2 “Frustrated Lewis Pairs” Metal-Free Catalytic Hydrogenation Future Considerations Acknowledgements References