gebhart/patrepeval huggingface dataset:
Tracking a system of multiple cameras on a rotating spherical robot (2017)

For many applications in the operation of a spherical robot, it is necessary to use optical devices to observe, track and monitor the robot's surroundings and environment. Estimating the rotation of a multiple camera system is crucial and can also be complex and computationally expensive. Demonstrating the multiple camera system with a shared central point can be simpler and require less computation. In this paper, we will demonstrate that a multiple camera system can be developed, an estimation of the movement of a wheel and sphere can be tracked, and the movement can be observed from a remote location.
Optogenetic control of protein binding using light-switchable nanobodies (2019)

Abstract A growing number of optogenetic tools have been developed to control binding between two engineered protein domains. In contrast, relatively few tools confer light-switchable binding to a generic target protein of interest. Such a capability would offer substantial advantages, enabling photoswitchable binding to endogenous target proteins in vivo or light-based protein purification in vitro . Here, we report the development of opto-nanobodies (OptoNBs), a versatile class of chimeric photoswitchable proteins whose binding to proteins of interest can be enhanced or inhibited upon blue light illumination. We find that OptoNBs are suitable for a range of applications: modulating intracellular protein localization and signaling pathway activity and controlling target protein binding to surfaces and in protein separation columns. This work represents a first step towards programmable photoswitchable regulation of untagged, endogenous target proteins. Highlights Opto-Nanobodies (OptoNBs) enable light-regulated binding to a wide range of protein targets. We identify an optimized LOV domain and two loop insertion sites for light-regulated binding. OptoNBs function in vivo : when expressed in cells and fused to signaling domains, OptoNBs enable light-activated and light-inhibited Ras/Erk signaling. OptoNBs function in vitro : Target proteins can be reversibly bound to OptoNB-coated beads and separated through size-exclusion chromatography.
TCR-mediated adhesion of T cell hybridomas to planar bilayers containing purified MHC class II/peptide complexes and receptor shedding during detachment (1996)

T cell recognition of foreign Ag/MHC class II complexes is sensitive down to approximately 100 complexes per cell or approximately 0.2 complexes/micron2. To better understand the physical basis of the recognition stage of Ag presentation, we examined adhesion of the lysozyme- specific T cell hybridoma, 3A9, to artificial bilayers containing covalent MHC class II/peptide complexes or adhesion molecules. Adhesion of 3A9 cells required a superphysiologic density of the MHC class II/peptide complex and was partly dependent on CD4; cells adhered but did not crawl. No adhesion was observed to bilayers containing MHC class II molecules without the lysozyme peptide. Activated 3A9 cells adhered and crawled on bilayers containing ICAM-1. The physical strength of contacts was tested with fluid shear. 3A9 cells adherent to bilayers containing MHC class II/peptide complexes shed their contact, which remained on the substrate and contained TCR. In contrast, 3A9 cells peeled from the ICAM-1 bilayer, and held firmly on LFA-1 bilayers; in a manner dependent on filamentous actin. When ICAM-1 and the MHC/peptide complexes were combined, the 3A9 cells adhered tightly and spread, but did not crawl, on the bilayers and TCR clustered at the center of the contact area. Physiologically, the TCR is unlikely to directly initiate adhesion. TCR clusters formed with the assistance of adhesion mechanisms may have to be shed to allow de-adhesion, and this may contribute to TCR down-regulation.
Design Strategies for Tripolarized Antennas with Wideband Characteristic (2020)

Two design strategies to construct wideband tripolarized antennas are summarized and presented in this paper. First is a design method for a low-profile structure, which can achieve up to 24% fractional bandwidth with a profile of only 0:09λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">min</sub> , (λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">min</sub> is the free-space wavelength at the minimum operating frequency). When the antenna height can be compromised, i.e. increasing to about 0:17λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">min</sub> , we will demonstrate how a substantially large fractional bandwidth of 80% can be achieved in an integrated structure.
Numerical studies of transient gain reduction process in a multi-wire proportional chamber (2011)

A gain reduction process caused by successive beam irradiation in a multi-wire proportional chamber was numerically investigated to clarify the relations between the gas gain variation and the ion density distribution. A numerical code was developed based on a two-dimensional drift-diffusion model in order to evaluate the ion and electron density distributions and the electric field variation caused by the space charge effect. In order to consider the gain reduction process which occurs under the high rate and successive irradiation, the simulations were performed for the time period of ∼10-100 μs, which is much longer than the time required for ions to travel from an anode to a cathode. The numerical simulation results showed that for the low gas gain regime of ∼10, quasi-stationary density distribution of the ions was formed by the high-rate beams of ∼10(8)-10(10) particles per second, and that the transient variation of the gas gain became constant after establishment of the quasi-stationary ion density distributions.
Bilateral lingual nerve injury following the use of the laryngeal mask airway (1998)

We report a 27‐year‐old patient who developed transient bilateral lingual nerve palsy following the use of a laryngeal mask to maintain the airway during a general anaesthetic for arthroscopy of the wrist.
Fermentation pathways and their interactions with photosynthesis in the marine diatom T. pseudonana: proteomic and biophysics approaches (2021)

The glycolysis associated with mitochondrial oxidative phosphorylation is the process by which the vast majority of eukaryotes produces ATP from sugar. Under hypoxic or anoxic conditions, fermentation pathways allow to maintain glycolytic activity by reducing alternative electron acceptors (other than O2) while generating various fermentation by-products. Anaerobic fermentation and photosynthesis coexist when organisms experience hypoxia or anoxia in their natural environment (e.g. marine sediments, eutrophic waters). In photosynthetic microeukaryotes, the detailed study of anaerobic metabolic pathways is limited to few freshwater model organisms such as Chlamydomonas reinhardtii (1). Based a previous genomic survey, Thalassiosira pseudonana, a centric marine diatom would contain a large variety of fermentation pathways (2). In our work, we studied the fermentation pathways and their interactions with photosynthesis in T. pseudonana. We first show that there are different waves of protein expression during the first 24 hours in anoxia in the dark. Several fermentative metabolites are also detected (H2, succinate), and our data suggest the existence of a bacterial fermentative pathway leading to butyrate production. The availability in photosynthetic electron acceptors is reduced but not null after 24h in anoxia. This suggests that at least one fermentative pathway is able to reoxidize photosynthetic electron acceptors, as it was previously shown for hydrogenase activity in C. reinhardtii (3). Finally, by comparing PSI and PSII activity, we evidence that a high and transient cyclic electron flow (CEF) around PSI is key to resume PSII electron transfer in anoxic condition. Overall, our results reveal regulatory mechanisms (CEF and fermentation pathways) that may help T. pseudonana cope with hypoxic or anoxic environments. References: (1) Muller, M. et al. (2012). Microbiol Mol Biol Rev, 76, 444-495 (2) Atteia et al. (2013). Biochim Biophys Acta - Bioenerg 1827: 210–223 (3) Godaux et al. (2013). Int. J. Hydrog. Energy, 38: 1826-1836
A Perspective on Physiologic Assessment of Coronary Stenoses in Series (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Cardiology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Cardiology journal
An Expedited Forwarding PHB (Per-Hop Behavior) (2002)

This document defines a PHB (per-hop behavior) called Expedited Forwarding (EF). The PHB is a basic building block in the Differentiated Services architecture. EF is intended to provide a building block for low delay, low jitter and low loss services by ensuring that the EF aggregate is served at a certain configured rate. This document obsoletes RFC 2598.
A New Paradigm for Understanding and Enhancing the Critical Heat Flux (CHF) Limit (2017)

Nearly a century of research on enhancing critical heat flux (CHF) has focused on altering the boiling surface properties such as its nucleation site density, wettability, wickability and heat transfer area. But, a mechanism to manipulate dynamics of the vapor and liquid interactions above the boiling surface as a means of enhancing CHF has not been proposed. Here, a new approach is implemented to limit the vapor phase lateral expansion over the heat transfer surface and actively control the surface wetted area fraction, known to decline monotonically with increasing heat flux. This new degree of freedom has enabled reaching unprecedented CHF levels and revealed new details about the physics of CHF. The impact of wickability, effective heat transfer area, and liquid pressure on CHF is precisely quantified. Test results show that, when rewetting is facilitated, the CHF increases linearly with the effective surface heat transfer area. A maximum CHF of 1.8 kW/cm<sup>2</sup> was achieved on a copper structure with the highest surface area among all tested surfaces. A model developed based on the experimental data suggests that the thermal conductivity of the surface structures ultimately limits the CHF; and a maximum CHF of 7-8 kW/cm<sup>2</sup> may be achieved using diamond surface structures.
The Occurrence of Flow Marks during Injection Molding of Linear Polyethylene (1997)

Abstract Injection molded parts often show several types of surface defect. It has been hypothesized that wall slip is associated with some of these defects. Wall slip of molten plastics has been observed above a certain critical shear stress in rheological measurements. The objectives of the proposed research were to analyze the occurrence of flow marks during the injection molding of linear polyethylene and evaluate its possible relation to wall slip. Various variables were studied in terms of their influence on flow mark formation: mold thickness, mold temperature, melt temperature, gate, injection speed and Teflon TM and silicone oil coatings. It was found that injection speed is the controlling factor for the generation of flow marks during injection molding of the linear polyethylenes studied. Since one of the resin studied had shown no tendency to slip in capillary flow experiments, and since a Teflon TM coating on the mold walls did not affect the occurrence of flow marks, we conclude that there is no relationship between wall slip and the generation of flow marks. Microscopic observation of molded surfaces suggests instead that flow marks result from the filamentaton and stretching of semi-solidified material in the neighborhood of the three-phase contact line.
A PELDOR-Based Nanometer Distance Ruler for Oligonucleotides (2004)

A pulsed electron paramagnetic resonance (EPR) spectroscopic ruler for oligonucleotides was developed using a series of duplex DNAs. The spin-labeling is accomplished during solid-phase synthesis of the oligonucleotides utilizing a palladium-catalyzed cross-coupling reaction between 5-iodo-2'-deoxyuridine and the rigid spin-label 2,2,5,5-tetramethyl-pyrrolin-1-yloxyl-3-acetylene (TPA). 4-Pulse electron double resonance (PELDOR) was then used to measure the intramolecular spin-spin distances via the dipolar coupling, yielding spin-spin distances of 19.2, 23.3, 34.7, 44.8, and 52.5 A. Employing a full-atom force field with explicit water, molecular dynamic (MD) simulations on the same spin-labeled oligonucleotides in their duplex B-form gave spin-spin distances of 19.6, 21.4, 33.0, 43.3, and 52.5 A, respectively, in very good agreement with the measured distances. This shows that the oligonucleotides adopt a B-form duplex structure also in frozen aqueous buffer solution. It also demonstrates that the combined use of site-directed spin-labeling, PELDOR experiments, and MD simulations can yield a microscopic picture about the overall structure of oligonucleotides. The technique is also applicable to more complex systems, like ribozymes or DNA/RNA-protein complexes, which are difficult to access by NMR or X-ray crystallography.
Understanding the relationship between olfactory‐specific quality of life, objective olfactory loss, and patient factors in chronic rhinosinusitis (2017)

In CRS, QOD-NS correlates with non-white race, depression, SNOT-22, and TDI score, with discrimination having the strongest correlation. The QOD-NS also appears to be a feasible tool for olfaction screening.
Single-Molecule DNA Amplification and Analysis Using Microfluidics (2010)

ADVERTISEMENT RETURN TO ISSUEPREVReviewSingle-Molecule DNA Amplification and Analysis Using MicrofluidicsChunsun Zhang and Da Xing*View Author Information MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China* To whom correspondence should be addressed. Mailing address: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, No. 55 Zhongshan Avenue West, Tianhe District Guangzhou 510631, P.R. China. Tel: +86-20-85210089. Fax: +86-20-85216052. E-mail: [email protected]Cite this: Chem. Rev. 2010, 110, 8, 4910–4947Publication Date (Web):April 15, 2010Publication History Received2 March 2009Published online15 April 2010Published inissue 11 August 2010https://pubs.acs.org/doi/10.1021/cr900081zhttps://doi.org/10.1021/cr900081zreview-articleACS PublicationsCopyright © 2010 American Chemical SocietyRequest reuse permissionsArticle Views6574Altmetric-Citations124LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluid dynamics,Genetics,Genomics,Liquids Get e-Alerts
THE DEVELOPMENT OF ANTI-ACNE PRODUCTS FROM EUCALYPTUS GLOBULUS AND PSIDIUM GUAJAVA OIL (2008)

The aims of this study were to determine the main chemical constituents of volatile oils from leaves of Eucalyptus globulus Labill. (eucalyptus) and Psidium guajava L. (guava) and to develop stable anti-acne preparations from these medicinal plants. Volatile oils from eucalyptus and guava leaves were extracted by hydrodistillation and then were analyzed of their main chemical components by means of GC/MS. The antimicrobial activity of the oils was determined by agar diffusion and micro-dilution methods. The GC/MS results showed that the main chemical constituents in the volatile oils from eucalyptus and guava leaves were gamma-terpinene and alpha-pinene. These volatile oils showed good antimicrobial activity against Propionibacterium acnes (MIC, MBC = 9.38 mg/ml for eucalyptus oil and MIC = 9.38 mg/ml, MBC = 37.50 mg/ml for guava oil). Further, oil- in-water creams incorporating 2% w/w eucalyptus oil and 4% w/w guava oils were prepared. Both of them had satisfied texture and exhibited good stability after stored at -4 ° C and 45 ° C alternately (freeze-thawing) for 4 cycles. In addition, they had antimicrobial activity against P. acnes determined by agar well diffusion method (inhibition zone = 8.0 and 9.0 mm for eucalyptus cream and guava cream, respectively) compared to commercial 5% benzoyl peroxide anti-acne gel.
Redesigned Curricula, Stringent Licensing Criteria, and Integrated Independence Are Conditions for a Bright Future for Nuclear Medicine in the United States (2023)

In a recent editorial entitled, “The Future of Nuclear Medicine in the United States,” Graham lists several major problems jeopardizing the future of the field in the United States ([1][1]). These include the dwindling number of active Accreditation Council for Graduate Medical Education–
The hostile environment of the aging human aorta and the smaller patient- their implications for the intra-aortic balloon (1994)

Intra-aortic balloon counterpulsation (IABC) has been a clinical modality since 1968. In the intervening years the patient population has become older, sicker and smaller. Modern balloon design permits the insertion of balloons into this evolving patient population and the balloon is increasingly threatened by the calcific plaque of the aging aorta and by the geometry of the shorter descending thoracic aorta in the smaller patient. Balloon sizing consideration and radiographic verification of balloon position within the aorta will reduce the incidence of balloon damage during IABC.
Modern developments in clinical perimetry. (1993)

Recent developments in automated perimetry have emphasized the adaptation of specialized psychophysical procedures for visual field testing to measure visual properties other than the detection of light. In addition, many new methods to quantitatively analyze visual field data have been generated. These new developments are promising for enhancing the diagnostic capabilities of automated perimetry in patients with glaucoma and patients suspected of having glaucoma. A review of some of the most recent advances in automated perimetry are presented.
Effects of glyphosate on Fusarium spp.: its influence on root colonization of weeds, propagule density in the soil, and crop emergence (1987)

Glyphosate is a broad spectrum herbicide that can lead to root rot like damage on crops. This study was undertaken to investigate the effect of glyphosate on the root-colonizing Fusarium spp. The research was conducted at two sites. Site one was densely covered with perennial weeds, and site two with annuals. At site one, spraying the weed cover with glyphosate increased (p &lt; 0.05) the level of colonization by Fusarium spp. in Ranunculus repens and Holcus lanatus, but not in Stellaria media and Plantago lanceolata. At site two, glyphosate enhanced colonization in Spergula arvensis, Stellaria media, Echinochloa crusgalli, and Chenopodium album, but not in Capsella bursa-pastoris and Polygonum persicaria. At both sites, the number of colony-forming units of Fusarium spp. per gram of dried soil was increased by the application of glyphosate. Nevertheless, crops subsequently sown in the field containing the annual weeds were not detrimentally affected by glyphosate treatment of these weeds.
Personal knowledge graph population from user utterances in conversational understanding (2014)

Knowledge graphs provide a powerful representation of entities and the relationships between them, but automatically constructing such graphs from spoken language utterances presents the novelty and numerous challenges. In this paper, we introduce a statistical language understanding approach to automatically construct personal (user-centric) knowledge graphs in conversational dialogs. Such information has the potential to better understand the users' requests, fulfilling them, and enabling other technologies such as developing better inferences or proactive interactions. Knowledge encoded in semantic graphs such as Freebase has been shown to benefit semantic parsing and interpretation of natural language utterances. Hence, as a first step, we exploit the personal factual relation triples from Freebase to mine natural language snippets with a search engine, and the resulting snippets containing pairs of related entities to create the training data. This data is then used to build three key language understanding components: (1) Personal Assertion Classification identifies the user utterances that are relevant with personal facts, e.g., “my mother's name is Rosa”; (2) Relation Detection classifies the personal assertion utterance into one of the predefined relation classes, e.g., “parents”; and (3) Slot Filling labels the attributes or arguments of relations, e.g., “name(parents): Rosa”. Our experiments using the Microsoft conversational understanding system demonstrate the performance of this proposed approach on the population of personal knowledge graphs.
Evaluation of the uptake of bioactive components from wheat-bran and wheat-aleurone fractions in healthy adults (2008)

Epidemiological evidence indicates that consumption of diets rich in whole grains is associated with a reduced incidence of a number of chronic diseases including heart disease and type 2 diabetes The mechanisms of protection, however, are unclear. Whole grains, particularly the bran and germ fractions of wheat, are rich in the vitamin E isomers aand g-tocopherol, which may protect against free radical damage, and are also good sources of folate, choline and betaine, which act as physiological methyl donors. The aim of the current study was to assess the post-meal uptake of tocopherols and these methyl donors after consumption of wheat-bran and wheat-aleurone test meals.