gebhart/patrepeval huggingface dataset:
Molecular and Functional Relevance of NaV1.8-Induced Atrial Arrhythmogenic Triggers in a Human SCN10A Knock-Out Stem Cell Model (2023)

In heart failure and atrial fibrillation, a persistent Na<sup>+</sup> current (I<sub>NaL</sub>) exerts detrimental effects on cellular electrophysiology and can induce arrhythmias. We have recently shown that Na<sub>V</sub>1.8 contributes to arrhythmogenesis by inducing a I<sub>NaL</sub>. Genome-wide association studies indicate that mutations in the <i>SCN10A</i> gene (Na<sub>V</sub>1.8) are associated with increased risk for arrhythmias, Brugada syndrome, and sudden cardiac death. However, the mediation of these Na<sub>V</sub>1.8-related effects, whether through cardiac ganglia or cardiomyocytes, is still a subject of controversial discussion. We used CRISPR/Cas9 technology to generate homozygous atrial <i>SCN10A</i>-KO-iPSC-CMs. Ruptured-patch whole-cell patch-clamp was used to measure the I<sub>NaL</sub> and action potential duration. Ca<sup>2+</sup> measurements (Fluo 4-AM) were performed to analyze proarrhythmogenic diastolic SR Ca<sup>2+</sup> leak. The I<sub>NaL</sub> was significantly reduced in atrial <i>SCN10A</i> KO CMs as well as after specific pharmacological inhibition of Na<sub>V</sub>1.8. No effects on atrial APD<sub>90</sub> were detected in any groups. Both <i>SCN10A</i> KO and specific blockers of Na<sub>V</sub>1.8 led to decreased Ca<sup>2+</sup> spark frequency and a significant reduction of arrhythmogenic Ca<sup>2+</sup> waves. Our experiments demonstrate that Na<sub>V</sub>1.8 contributes to I<sub>NaL</sub> formation in human atrial CMs and that Na<sub>V</sub>1.8 inhibition modulates proarrhythmogenic triggers in human atrial CMs and therefore Na<sub>V</sub>1.8 could be a new target for antiarrhythmic strategies.
Towards understanding and improving escape probabilities in PPM (2002)

The choice of expressions for the coding probabilities in general, and the escape probability in particular, is of great importance in the family of prediction by partial matching (PPM) algorithms. We present a parameterized version of the escape probability estimator which, together with a "compactness" criterion, provides guidelines for the estimator design given a "representative" set of files. This parameterization also makes it possible to adapt the expression of the escape probability during one-pass coding. Finally, we present results for one such compression scheme that illustrates the usefulness of our approach.
Novel Plasma Approach for the Synthesis of Highly Fluorinated Thin Surface Layers (2002)

SF6-RF-plasma environments were employed for the generation of SFx-oxygen-scavenger species, and used for the synthesis of thin highly fluorinated paper, poly(acrylic acid) (PAA), polyethylene (PE), plasma-oxidized polyethylene (OxPE), and poly(vinyl alcohol) (PVA) surface layers. The concentration of fluorine atoms in the SF6-plasma was monitored using actinometry. The relative surface atomic concentrations and the surface characteristics of the plasma-modified substrates were evaluated using electron spectroscopy for chemical analysis, contact angle goniometry, and atomic force microscopy. It was demonstrated that the plasma-produced SFx species initiate simultaneous intense surface fluorination and oxygen extraction mechanisms, and that −C−O, −CO, and −COOH functionalities are converted into −CF, −CF2, and −CF3 groups, respectively. Contact angle (CA) investigations indicate that longer plasma exposure times result in very high, stable contact angle values. It is suggested that the low stability in time of CA values for the paper samples can be related to the porous nature of the substrates and macromolecular motion. The results of these investigations open up novel ways for the plasma-enhanced synthesis of thin membrane structures with dissimilar surfaces, which might play a significant role in the area of membrane-assisted catalysis (e.g., Nafion-type structures). It is also suggested that preoxidized polymer surfaces might lead to more efficient and uniform surface fluorination processes under SF6-plasma environments in comparison to their non-oxidized counterparts. By selecting plasma-enhanced oxidation reaction mechanisms, which selectively result in the formation of −CO and −COOH groups, novel routes open up for the synthesis of CF2- and CF3-bearing surface layers.
Binding of GM1‐ganglioside to a synthetic peptide derived from the lysosomal sphingolipid‐activator‐protein saposin B (1994)

Saposin B is a lysosomal sphingolipid-activator-protein which activates GM1-ganglioside hydrolysis by lysosomal beta-galactosidase. To identify the structural elements of saposin B implicated in sphingolipid binding, we studied a synthetic peptide corresponding to a predicted alpha-helix, sapB-18, spanning residues 52 to 69 of saposin B. The circular dichroism spectrum of sapB-18 at pH 4.4 was consistent with a 44% alpha-helix content. As shown by intrinsic Tyr fluorescence studies of sapB-18, this peptide binds the GM1-ganglioside with a Kd of about 7 microM. Thus, we suggest that a putative amphipathic alpha-helix between residues 52 and 69 of saposin B plays a major role in the recognition and binding of GM1-ganglioside by saposin B.
HRP-4 Walks on Soft Feet (2020)

The majority of humanoid robots adopt flat feet, a choice that can limit their performance when maneuvering over uneven terrains. Recently, a soft robotic foot designed to adapt to the ground was proposed to overcome part of these limitations. This paper presents the results of testing two such feet on the humanoid robot HRP-4, and compares them to what obtained with the original flat feet of the robot. After describing the SoftFoot and how it has been adapted to the robot, the biped is tested while balancing, stepping and walking. Tests are carried out on flat ground and on obstacles of different heights. For comparison purposes, the original HRP-4 controller has been used for both types of feet with no changes (except for re-evaluation of the CoM position). Analysis of the ankle pitch angle, ankle pitch torque, knee pitch angle, knee pitch torque, waist roll angle and waist pitch angle, show a substantial improvement in obstacle negotiation performance of HRP-4, when using the SoftFoot, even without optimizing the controller to exploit the SoftFoot features.
The research of Fas /FasL expression and apoptosis in experimental autoimmune hepatitis mouse model (2013)

Objective: To establish experimental autoimmune hepatitis(EAH) mouse model and observe Fas /FasL expression and cell apoptosis in autoimmune liver tissue damage.Methods: The syngeneic liver antigen emulsified in complete Freund's adjuvant was injected intraperitoneally into adult female C57BL /6 mice twice.Liver injury was assessed by serum ALT,AST and liver histopathological changes.The autoantibody was detected by immunofluorescence.Using real-time quantitative-polymerase chain reaction(Realtime-PCR) and immunohistochemisty to detect gene transcription and protein expression of Fas and FasL in the mouse liver,and cell apoptosis was detected in liver tissue by TUNEL.Results: In liver tissue of model mice after 28-days modeled,Fas and FasL mRNA and protein levels which mainly expressed in liver cells and infiltrating lymphocytes were significantly enhanced(P 0.05).No abnormal changes in normal mouse liver tissue while model mice showed scattered or focal infiltration of lymphocytes by light microscopy.The TUNEL-positive hepatocytes and lymphocytes can be seen in the inflammatory regional centers and around,and apoptosis index(AI) in the liver tissue of the model group were significantly elevated.Conclusion: Inflammatory infiltration and apoptosis coexist in liver tissue of experimental autoimmune hepatitis.Cell apoptosis mediated by the Fas / FasL path is associated with the development of autoimmune hepatitis.
Genomic and proteomic responses to drought stress and biotechnological interventions for enhanced drought tolerance in plants (2022)

Drought stress, an inevitable factor due to global climate change, hampers plant biomass production and overall yield. Drought mediated stress sensitivity exerts multi-dimensional effect on transcriptional and proteomic variations leading to changes in morphological, physiological, metabolic and hormonal responses. To cope up water scarcity, plants adapt several drought avoidances or tolerance mechanisms including biochemical, physiological and gene regulatory networks, leading to their effective survival. Genomic intervention modulates the defensive strategies of drought-related to phytohormones (auxins, cytokinins, ethylene, ABA and brassinosteroids), signalling molecules (e.g. nitric oxide), transcription factors, and transcriptional and translational modifications. Further, proteomic modulation is allied with antioxidant defence, photosynthesis, respiration, stomatal conductance, cell signalling and post-translational modifications of proteins. These factors exhibit strong mitigation strategies related to the acclimatisation of plants in response to water deficit. This review presents mechanistic explanations to draught stress following genomic and proteomic approaches, and suggests effective morpho-physiological, biochemical, and bio-technological strategies to overcome the negative impacts of drought stress. It highlights current knowledge, potential strategies and future possibilities to improve drought tolerance and crop yield.
Release of Oxytocin and Vasopressin in Lactating Rats After Injection of Carbachol into the Third Ventricle (1971)

Injection of carbachol into an indwelling third ventricular cannula evoked milk ejection in anesthetized lactating rats as measured by increased intraductile mammary pressure. There was no alteration in blood pressure. Similar doses were ineffective when injected systemically, either intravenously or into the carotid artery. Larger doses of carbachol given intravenously evoked milk ejection, probably via a direct effect on the mammary myoepithelium. The effect of intraventricular injection of carbachol was blocked by prior injection of atropine into the third ventricle. These results were interpreted to mean that a cholinergic synapse lies in the central pathways controlling oxytocin release. Intraventricular carbachol appeared to be less effective in evoking oxytocin release in hydrated rats. In the hydrated animals, intraventricular carbachol evoked an antidiuresis accompanied by increased concentrations of Na+ and K+, indicative of vasopressin release. Antidiuresis frequently occurred in the absence of milk ejection, which indicates that the threshold for vasopressin release to this stimulus is sometimes less than that for oxytocin release. An increase in excretion of sodium and an increase in the Na+/K+ excretion ratio also occurred after intraventricular carbachol, but were not mimicked by injection of vasopressin or oxytocin.
Temperature-dependent Luminescence Spectra of Ruddlesden-Popper Quasi-2D Perovskites (2023)

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3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation (2012)

Fluorescence and phosphorescence lifetime imaging are powerful techniques for studying intracellular protein interactions and for diagnosing tissue pathophysiology. While lifetime-resolved microscopy has long been in the repertoire of the biophotonics community, current implementations fall short in terms of simultaneously providing 3D resolution, high throughput, and good tissue penetration. This report describes a new highly efficient lifetime-resolved imaging method that combines temporal focusing wide-field multiphoton excitation and simultaneous acquisition of lifetime information in frequency domain using a nanosecond gated imager from a 3D-resolved plane. This approach is scalable allowing fast volumetric imaging limited only by the available laser peak power. The accuracy and performance of the proposed method is demonstrated in several imaging studies important for understanding peripheral nerve regeneration processes. Most importantly, the parallelism of this approach may enhance the imaging speed of long lifetime processes such as phosphorescence by several orders of magnitude.
Bcl-2 protein expression correlates with nodal status in non small cell lung cancer. (1999)

We investigated the immunohistochemical expression of bcl-2 in 55 non small cell lung cancer patients in order to understand if the altered expression of this gene is involved in the development of this kind of neoplasm. Our results showed bcl-2 immunopositivity to be a frequent event in non small cell lung cancer (54.5%) with a higher expression in squamous carcinomas compared to adenocarcinomas (p = .04). In addition, we found a positive correlation between bcl-2 expression levels and nodal status (p < .003). We suggest that bcl-2 immunostaining could be considered as marker of loco-regional invasivity.
Monocular visual odometry: Sparse joint optimisation or dense alternation? (2017)

Real-time monocular SLAM is increasingly mature and entering commercial products. However, there is a divide between two techniques providing similar performance. Despite the rise of ‘dense’ and ‘semi-dense’ methods which use large proportions of the pixels in a video stream to estimate motion and structure via alternating estimation, they have not eradicated feature-based methods which use a significantly smaller amount of image information from keypoints and retain a more rigorous joint estimation framework. Dense methods provide more complete scene information, but in this paper we focus on how the amount of information and different optimisation methods affect the accuracy of local motion estimation (monocular visual odometry). This topic becomes particularly relevant after the recent results from a direct sparse system. We propose a new method for fairly comparing the accuracy of SLAM frontends in a common setting. We suggest computational cost models for an overall comparison which indicates that there is relative parity between the approaches at the settings allowed by current serial processors when evaluated under equal conditions.
Produktionsangepasste Konstruktion*/Production-adjusted design for hot die forging processes (2016)

Der Fachaufsatz stellt eine Methode vor, mit der bereits während der Konstruktion von Schmiedewerkzeugen produktionsspezifische Informationen berücksichtigt werden. Für die produktionsgerechte Konstruktion wird unter Berücksichtigung von Fertigungsmitteldaten und daraus abgeleiteten Kennzahlen eine Zusatzanwendung innerhalb einer CAD-Umgebung entwickelt. Diese gestattet bereits in der frühen Konstruktionsphase eine geführte Fertigungsmittelauswahl und automatisierte Werkzeuganpassung. &amp;nbsp; A methodology is presented providing production-specific information for the design of hot die forging tools. For a production-adjusted design an additional prototypical software application within a conventional CAD-framework is developed including the properties of the production resources and derived indicators. The application allows already in the early design stage a guided selection of production resources and an automatic geometric adaption of the forging tools.
The Interface of Genetics and Public Health: Research and Educational Challenges (2000)

As the target date for the sequencing of the human genome approaches, there is growing recognition that public health practice, research, and education will be impacted by new genetic technologies and information and that a multidisciplinary approach is required. Research in the emerging field of public health genetics encompasses a broad range of disciplines and will increasingly involve the interactions among the investigators in these fields. An overview of these areas of research is provided, with illustrative examples. Education in public health genetics needs to address a variety of audiences, including public health graduate students and practitioners, students from related disciplines, and health care professionals. Two new graduate programs at the Universities of Michigan and Washington and training opportunities for public health professionals are described. These educational efforts must be ongoing so that the potential of genetic technology and information can be appropriately used to benefit the health of all.
Os agregados de informação - memórias, esquecimento e estoques de informação (2000)

Conceituamos como estoques de conteudos de informacao toda reuniao de estruturas de informacao. Estoques de informacao representam, assim, um conjunto de itens de informacao organizados (ou nao), segundo criterio tecnico, dos instrumentos de gestao da informacao e com conteudo que seja de interesse de uma comunidade de receptores. Quanto mais o estoque de informacao estiver codificado em uma metalinguagem mais estara ocultando a informacao completa em linguagem natural. Um estoque de itens codificados servira, principalmente, para direcionar o receptor para a informacao util, mas estabelece a necessidade de haver um duplo fluxo de comunicacao, um modelo de linguagem que se sobrepoe a natural. Como decorrencia das condicoes tecnicas e economicas, produzidas pela peculiar ambiencia de convivio da oferta e demanda em unidades de estoques de informacao, existe um reflexo ocasionando circunstâncias politicas que afetam a distribuicao da informacao e a potencial geracao do conhecimento no individuo e na sociedade. No contexto politico esta distribuicao e afetada pois o produtor de informacao tendera a transferir produtos que minimizem uma condicao de ineficiencia operacional do seu estoque, em detrimento de uma situacao que maximize a expectativa de qualidade de informacao da demanda dos seus usuarios. Aqueles que detem o poder sobre os estoques institucionais de informacao tambem detem o poder sobre a sua administracao e distribuicao e consequentemente sobre o conhecimento gerado na sociedade e o seu potencial de desenvolvimento. O produtor de informacao, proprietario dos estoques, decide sobre quais os itens de informacao devem ser armazenados e quais as estrategias para a sua distribuicao a sociedade. Decide, ainda, sobre o empacotamento tecnologico para a sua distribuicao, considerando que alguns dos canais de distribuicao sao tao intensivos na utilizacao de tecnologia, que podem direcionar a propria estrategia na transferencia da informacao. A gestao dos estoques de informacao pode ser tao autoritaria quanto as politicas que a orientam. Sera tanto mais autoritaria quanto maior for o poder de oferta sobre a demanda colocada pelos receptores.
ESSenTIAL: EPIXfab services specifically targeting (SME) industrial takeup of advanced silicon photonics (2012)

ePIXfab brings silicon photonics within reach of European small and medium sized enterprises, thereby building on its track record and its integration into Europractice. To this end, ePIXfab offers affordable access to standardized active and passive silicon photonic IC and packaging technology, a path from design to manufacturing and hands-on training. Based on a consortium of major research institutes with silicon photonics expertise, ePIXfab reaches out to European industry and supports them to evaluate silicon photonics in the context of concrete applications and markets. In order to ensure low-cost, quick access and scalability to manufacturing, the maturity of silicon photonic IC technology is enhanced by setting up a library of generic devices, a level of process and device benchmarking and a well maintained design flow. For the first time, devices in a standard package are offered to facilitate measurements. Training programs on the IC and packaging services are also offered, including hands-on training in making designs. Maturity, standardization and sustainability are driven by a steadily growing worldwide user base.
A two-dimensional detailed ion channel model of abnormal cardiac action potential propagation (2002)

Cardiac action potential propagation is modeled with a two-dimensional simulation that includes full Luo-Rudy (LRd) ion channel dynamics. Preliminary results show that the L-type calcium current is dependent on the curvature of the propagating wavefront, and that, in the case of spiral wave reentry, calcium-induced calcium release exhibits complex temporal behaviour including occasional large releases which occur on the boundary of the spiral wave core. The core region also exhibits significantly lower intracellular calcium concentrations. The computer model uses a simple forward Euler method in combination with a variable, multiple. The algorithm thereby allows the desktop modeling of a reasonable size spatial region while simultaneously providing for a detailed representation of the action potential upstroke. Attempts to reduce the runtime of the code, including parallelization issues, are also discussed.
The determinants of mobile shopping mall apps adoption intention in Malaysia: An empirical investigation (2016)

This study explores the key issues on mobile shopping mall apps (MSMAs) adoption and used a theoretical framework based on the value-based adoption model (VAM) to analyze the perceived value relations to MSMAs adoption behavior toward MSMAs in Malaysia. The factors that significantly direct affect perceived value toward MSMAs are usefulness, enjoyment, technicality, and fees. While perceived value had a significantly affect MSMA adoption. Surveys were carried out to shoppers at major shopping malls at Klang Valley of Malaysia. Data analysis was performed using the linear regression approach. The result provides useful information for shopping-mall operators who intent to provide value added services to their shoppers and enhance the competitive position in the shopping mall industry. The results showed that the VAM was found to be useful in explaining the shoppers' adoption intention of using MSMA in Malaysia.