gebhart/patrepeval huggingface dataset:
Antisense-mediated exon-skipping to induce gene knockdown. (2012)

Exon-skipping antisense oligonucleotides (ASOs) can be used to knockdown the expression of an undesired gene or specific gene isoform. This chapter discusses the potential therapeutic applications of the technique and provides a sample protocol for inducing exon-skipping in Apolipoprotein B in vitro, as well as a protocol for quantifying exon-skipping using real-time PCR.
Ecophyto R&D. Vers des systèmes de culture économes en pesticides. Volet 1. Tome I : méthodologie générale (2009)

Étude menée par l’Inra à la demande du ministère de l’Écologie, de l’Énergie, du Développement durable et de la Mer et du ministère de l’Alimentation, de l’Agriculture et de la Pêche. Ecophyto R&D vers des systèmes de culture économes en produits phytosanitaires, volet 1
Hybrid transport solutions for TDM/data networking services (2002)

There is a growing demand for native data transport services for enterprises and corporations across public transport networks. Equipment vendors have begun to incorporate a variety of LAN and storage area network interfaces, notably Ethernet, Fibre Channel/FICON, and ESCON, on traditional metro and long-haul transport equipment. Embracing Ethernet and SAN technology enables the introduction of flexible high-capacity transport services optimized for data networking. Transport operators may thus offer both enterprise-centric connectivity services, such as transparent LAN connectivity and virtual LAN services, as well as traditional bandwidth services, such as private lines, while preserving the operations and management infrastructure of the existing public networks. We discuss the benefits of a hybrid Ethernet/TDM transport solution.
Silicon Dissolution from Microporous Silicon Substituted Hydroxyapatite and its Effect on Osteoblast Behaviour (2006)

In this study silicon release from SiHA into tissue culture medium was investigated under static and semi-dynamic conditions. The effect of silicon release under semi-dynamic conditions on alkaline phosphatase activity (ALP) and collagen I (CICP) expression by osteoblast like cells (HOS TE85) was also examined. Under static conditions a low level of silicon was released within 24 hours, this initial level dropped over 3-7 days but subsequently increased again by 10-14 days. Under semi-dynamic conditions silicon was released within 24 hours and was subsequently reduced with each medium change until equilibrating at close to 0 after 10 days. ALP and CICP showed significant variation in expression between culture conditions. In direct contact with SiHA ALP peaked at day 10 and CICP was constantly elevated. Cells grown in the presence of but not on SiHA expressed progressively decreasing levels of ALP from 7-14 days, with CICP peaking at day 10. On thermanox (TMX) ALP constantly increased and CICP peaked at day 10. The results indicate that silicon leaches out of the lattice of the SiHA crystal structure but may also be reprecipitated onto the substrate. We have also demonstrated that Si influences osteoblast metabolism and differentiation whether it is available as free silicon or 'bound' in the apatite lattice.
Study on treatment of H22 liver cancer using 10-HCPT-Ioaded microbubbles and ultrasound-targeted destruction (2009)

Objective To prepare lipid-coated ultrasound microbubbles containing 10-HCPT(HLM) and explore the antitumor effects on mice xenografed H22 solid tumor using the technique of ultrasound-mediated HLM destruction. Methods Sixty-four tumor-bearing mice were radomly divided into A and B groups. Each group was divided into four groups again and administered respectively by tail vein with HI.M, non-drug-loaded microbubbles,10-HCPT and saline once a day. Ultrasound irradiation was applied on the tumor sites immediately after injection. After 7 days of consecutive treatment, all mice in group A were sacrificed and the tumors were harvested to measure weights. The tumor inhibition rate was calculated by weights. The tumor microvessel density (MVD) was detected by immunohistochemical staining. The tumor growth curve was depicted according to volumes. The survival time of mice in group B was recorded. Results The tumor inhibition rate was the highest in HLM group while this group's MVD was the lowest. Survival time in HLM group and 10-HCPT group were obviously longer compared with the control group,while no statistic difference was observed between the two groups. There was no statistic difference between the group of non-drug-loaded microbubbles and the control group. Conclusions Ultrasound irradiation mediates HLM destruction so that the drug is released from the vihicles at the same time, which can significantly enhance the tumor inhibition effect of 10-HCPT on the H22 tumor. This technique is expected to be adopted as a novel tool for liver cancer chemotherapy.

Key words: 
Ultrasonography;  Microbubbles ; Liver neoplasms;  experimental ;  Topotecan
New schemes for trellis source coding at various rates (2004)

Some earlier trellis source coding methods are extended to new sources and new rates above and below one. The new schemes have in common a code design based on branch correlation and an encoder based on both the Viterbi algorithm and tailbiting MAP techniques, The results from these simple schemes often exceed that of all previous methods with similar complexity.
In situ enzymatic template replication on DNA microarrays (2023)

DNA microarrays are very useful tools to study the realm of nucleic acids interactions at high throughput. The conventional approach to microarray synthesis employs phosphoramidite chemistry and yields unmodified DNA generally attached to a surface at the 3' terminus. Having a freely accessible 3'-OH instead of 5'-OH is desirable too, and being able to introduce nucleoside analogs in a combinatorial manner is highly relevant in the context of nucleic acid therapeutics and in aptamer research. Here, we describe an enzymatic approach to the synthesis of high-density DNA microarrays that can also contain chemical modifications. The method uses a standard DNA microarray, to which a DNA primer is covalently bound through photocrosslinking. The extension of the primer with a DNA polymerase yields double-stranded DNA but is also amenable to the incorporation of modified dNTPs. Further processing with T7 exonuclease, which catalyzes the degradation of DNA in a specific (5'→3') direction, results in template strand removal. Overall, the method produces surface-bound natural and non-natural DNA oligonucleotides, is applicable to commercial microarrays and paves the way for the preparation of combinatorial, chemically modified aptamer libraries.
High Union Rates and Function Scores at Midterm Followup With Ankle Arthrodesis Using a Four Screw Technique (2009)

Level IV, therapeutic study. See Guidelines for Authors for a complete description of levels of evidence.
Asymmetric Trienamine Catalysis for the Construction of Structurally Rigid Cyclic α,α‐Disubstituted Amino Acid Derivatives (2011)

2,4-Dienals are shown to undergo highly stereoselective Diels–Alder reactions with olefinic azlactones by asymmetric trienamine catalysis. The obtained cycloadducts are easily transformed into the corresponding cyclic α,α-disubstituted N-protected amino acid methyl esters under mild reaction conditions. Furthermore, it is demonstrated that in situ ligation of the crude cycloaddition products with amino acid hydrochloride salts leads to the formation of non-natural dipeptide motifs in a one-pot fashion.
Mild intermittent hypoxia exposure induces metabolic and molecular adaptations in men with obesity (2021)

This study is registered at the Netherlands Trial Register (NL7120/NTR7325).
Capsaicin-sensitive nerves mediate esophageal mucosal protection. (1991)

The esophageal mucosa is exposed to damaging agents both by ingestion and reflux. Using our in vivo rabbit model of esophagitis, we have observed that acute luminal exposure (within 1 to 5 minutes) to potentially harmful agents, such as acid, bile, or ethanol, induces a rapid increase in mucosal blood flow; whereas prolonged exposure (10 to 60 minutes) results in mucosal injury and ablation of blood flow. We have also shown that capsaicin-sensitive mucosal afferent nerves can modulate esophageal blood flow. These findings led us to hypothesize that the reactive increase in blood flow induced by luminal agents represents a mechanism of protection mediated by capsaicin-sensitive nerves. The objective of these experiments was to determine if luminal capsaicin, a specific probe for visceral afferent nerves, could both preserve mucosal blood flow and protect against ethanol injury. Rabbits were subjected to luminal instillation of 50% ethanol with or without 1% capsaicin. Blood flow was measured with microspheres at baseline and after 2 and 10 minutes. Rabbits exposed only to ethanol developed severe mucosal injury coincident with near ablation of mucosal blood flow. In contrast, rabbits exposed to ethanol with capsaicin showed protection of the epithelium with a sixfold increase in mucosal blood flow. We conclude that capsaicin-sensitive nerves in the esophagus are local effectors of mucosal protection by virtue of preserving blood flow.
BGA lifetime prediction in JEDEC drop tests accounting for copper trace routing effects (2010)

Experimental drop test results of 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">nd</sup> -level assemblies can be influenced by numerous impact factors. The explicit definition of drop testing conditions by the JEDEC standard JESD22-B111 was intended to create a highly repeatable, and thus comparable, experimental setup. Recent developments showed, however, shifting failure modes from component to PCB side. Comprehensive drop tests were executed with 3 different memory packages. Failure analysis surprisingly found electrical fails of the assemblies on PCB side with broken copper traces next to the PCB pads. Detailed investigations showed a strong influence of the copper trace routing on their failure probability. Broken copper traces were only found when their routing direction was aligned to the dominating PCB deflection. The new experimental insights were proven by a two steps simulation approach. In the first step, an experimentally calibrated and validated 3-D JEDEC board model has been set up. In the second step, the copper trace routing effect was investigated by a 3-D sub-model of a single BGA component. The direction of copper trace routing showed a clear effect on the plastic copper strain. Simulation results were able to prove the experimental observations. Using these results, reliability modeling was started for two drop test configurations: 4-screw and 6-screw board clamping. In both cases, the sequence of failure of the components on the board was matched by using a combined criterion of plastic strain-rate and resultant force integral. The lifetime model based on this criterion was able to predict the experimental cycles-to-failure with less than 25% deviation.
Engineering-reflected phase in Fabry–Perot sensors with resonant mirrors (2013)

Fabry-Perot cavities made with photonic crystal (PC) mirrors and other resonant structures exhibit nontraditional characteristics due to the strong wavelength dependence of their reflected phase. This Letter describes how engineering the phase of PC mirrors enables sensors that are tolerant to variations in laser center frequency and line width. Reflection spectra measurements of Fabry-Perot cavities made with PC mirrors were collected as a function of wavelength and cavity length, providing experimental verification of theory and simulations.
Abstract P180: Effect Of Sunitinib Treatment On Skin Sodium Accumulation In Patients With Metastatic Renal Cancer: A Pilot Study (2020)

Angiogenesis is required for invasive tumor growth and metastasis which is controlled mainly by vascular endothelial growth factors (VEGF). Novel strategies for cancer treatment target VEGF signaling. These agents are featured by adverse events including hypertension. New concepts suggest that besides the kidneys, the skin also plays a role in body sodium homeostasis and blood pressure regulation by a VEGF-C–dependent buffering mechanism. Here, we tested the hypothesis that changes in blood pressure correspond to tissue sodium accumulation during sunitinib treatment of metastatic renal cell carcinoma patients (https://clinicaltrials.gov/identifier: NCT04368546; Charité ethical approval EA1/044/15).Male patients (n=4) took sunitinib according to the standard treatment protocol, 50 mg once daily, taken for 4 weeks followed by a 2-weeks off treatment period. Measurements were performed at baseline (before sunitinib treatment), and over a complete on-off-on period. Tissue sodium content was measured using non-invasive 23Na-MRI; skin sodium was measured in a group (n=5) of age-matched healthy subjects, as well. Blood pressure was measured according to AHA guidelines. Blood withdrawal followed after ca. 45 minutes of sitting to measure VEGF-A, VEGF-C, endothelin-1, renin and aldosterone levels.Elevated systolic blood pressure under sunitinib treatment decreased to the baseline level in the off-treatment phase (130.5 mmHg vs 117.5 mmHg, respectively, p&lt;0.05). Plasma endothelin-1 levels mirrored directly and VEGF-A levels inversely the blood pressure changes. Baseline skin sodium content was similar to the skin sodium level of healthy controls. Skin sodium content was elevated after the first on-treatment phase and stayed high in all the following measurements in comparison to control subjects. Patients had high baseline VEGF-C levels that decreased after the first treatment with sunitinib and stayed low independently of further treatment phase.In conclusion, sunitinib treatment suppresses VEGF-C levels permanently which is associated with sustained elevated skin sodium levels. Furthermore, we confirmed the association between plasma endothelin-1 levels and blood pressure changes during sunitinib treatment protocol.
Additive models in high dimensions (1999)

We discuss some aspects of approximating functions on high-dimensional data sets with additive functions or ANOVA decompositions, that is, sums of functions depending on fewer variables each. It is seen that under appropriate smoothness conditions, the errors of the ANOVA decompositions are of order $O(n^{m/2})$ for approximations using sums of functions of up to $m$ variables under some mild restrictions on the (possibly dependent) predictor variables. Several simulated examples illustrate this behaviour.
Broadband ZnO Single-Nanowire Light-Emitting Diode (2006)

We present a novel technique for reliable electrical injection into single semiconductor nanowires for light-emitting diodes and lasers. The method makes use of a high-resolution negative electron-beam resist and direct electron-beam patterning for the precise fabrication of a metallic top contact along the length of the nanowire, while a planar substrate is used as a bottom contact. It can be applied to any nanowire structure with an arbitrary cross section. We demonstrate this technique by constructing the first zinc oxide single-nanowire light-emitting diode. The device exhibits broad sub-bandgap emission at room temperature.
Hepatoprotective effect of water extract from Chrysanthemum indicum L. flower (2013)

HCIF inhibited bioactivation of CCl4-induced hepatotoxicity and downregulates CYP2E1 expression in vitro and in vivo.
PageRank revisited (2006)

PageRank, one part of the search engine Google, is one of the most prominent link-based rankings of documents in the World Wide Web. Usually it is described as a Markov chain modeling a specific random surfer. In this article, an alternative representation as a power series is given. Nonetheless, it is possible to interpret the values as probabilities in a random surfer setting, differing from the usual one.Using the new description we restate and extend some results concerning the convergence of the standard iteration used for PageRank. Furthermore we take a closer look at sinks and sources, leading to some suggestions for faster implementations.
Parkinson’s Disease: Are PINK1 Activators Inching Closer to the Clinic? (2023)

The activation of PINK1 by small molecules has emerged as a promising strategy in treating Parkinson's disease (PD). Recent progress in this area has raised excitement around PINK1 activators as PD treatments, and herein we offer insight into these developments and their potential to deliver much needed novel PD treatments.
Influence of Diet and/or Exercise on Body Composition and Cardiorespiratory Fitness in Obese Women (1998)

The purpose of this study was to measure the influence of diet, exercise, or both on body composition and cardiorespiratory fitness in obese women. Ninety-one obese subjects were randomized into one of four groups: diet (D) (4.19-5.44 MJ or 1,200-1,300 kcal/day), exercise (E) (five 45-min sessions at 78.5+/-0.5% maximum heart rate), exercise and diet (ED), and controls (C). Maximal aerobic power and body composition were measured in all subjects before and after a 12-week diet intervention period. Subjects in D and ED lost 7.8+/-0.7 and 8.1+/-0.6 kg body mass, with no significant change for E relative to C. Losses of percent body fat and fat mass were significantly greater in D and ED but not in E relative to C. The change in VO2max was greater in ED and E but not D when compared to C. Results indicate that moderate aerobic exercise training during a 12-week period has no discernible effects on body composition but does improve cardiorespiratory fitness in dieting obese women.
7,9-Dimethyl-3-phenylnaphtho[1,2-b]furan-4,5-dione (2007)

In the title compound, C20H14O3, π–π stacking inter­actions [3.474 (2)–3.789 (2) Å] dominate the crystal structure. The dihedral angle between the phenyl ring and fused-ring system is 32.3 (3)°.
Big data environment for geospatial data analysis (2016)

Geo-information system produces huge and complex geospatial data with the process of collecting real time data through sensors devices. Ground surveying, remote sensing, mobile mapping are the major applications which produces geo-spatial data. There are many other applications like geo-located sensors, geo-tagged web contents, global navigation satellite system(GNSS), volunteered geographic information(VGI) tracking and so on. However, many issues would be answered when we reuse these huge data by applying the traditional data analysis tools. Big data is usually collections of huge data sets with sizes that exceed the storage capacity. This difficulty of storage and advantage of Big data handling tools availability, the data can be captured, managed and processed easily within optimal elapsed time. The hadoop is one of the framework with rich ecosystem which provides the distributed data storage through Hadoop Distributed File System (HDFS) and parallel processing through Map Reduce mechanism.
Monolithische Polymere als Trägermaterial für Zellkultivierung, Zelldifferenzierung und Tissue‐Engineering (2008)

Gewebewachstum im Rahmen von Polymeren: Durch elektronenstrahlinitiierte radikalische Polymerisation oder ringöffnende Metathesepolymerisation wurden biokompatible, hoch poröse, monolithische Gerüstmaterialien hergestellt, an denen in vitro dreidimensionale Knochen- und Fettgewebsäquivalente aufgebaut werden konnten. Mesenchymale Stammzellen ließen sich auf den Materialien zur Proliferation bringen und konnten nachweislich in Osteoblasten und Adipozyten differenziert werden. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Characterization of Spam Advertised Website Hosting Strategy (2009)

This paper surveys three months of spam data and investigates the hosting strategy of spam domains that are used to sell pharmaceutical, luxury goods and sexual enhancement tools. Thousands of domains have been found and most of them use wildcard DNS records to support non-existing machine names. The hosting IP addresses are much fewer than the number of domains, with a large number of domains hosted on a limited number of hosts. The majority of these heavily-used hosts reside in networks outside the USA. These hosts are stable and have good connectivity and availability. As a result, many domains on these hosts are alive for the entire three-month investigation period. The hosting IP addresses began to move in late March. The new IP addresses, however, are still in the same network range as the old IP addresses. The result suggests that further investigation on spam web hosting will be beneficial in disrupting the spamming network. 1.
Automated Langmuir trough with epifluorescence attachment (1985)

The authors describe an inexpensive automated Langmuir trough system. It was designed to achieve controlled transfer of monolayer and thin multilayer films of amphiphilic molecules from an air-water interface onto hydrophobic and hydrophilic substrates. The system incorporates an epifluorescence attachment for in situ observation of spread films during compression.
Deep learning of invariant spatio-temporal features from video (2010)

We present a novel hierarchical and distributed model for learning invariant spatio-temporal features from video. Our approach builds on previous deep learning methods and uses the Convolutional Restricted Boltzmann machine (CRBM) as a building block. Our model, called the Space-Time Deep Belief Network (ST-DBN), aggregates over both space and time in an alternating way so that higher layers capture more distant events in space and time. The model is learned in an unsupervised manner. The experiments show that it has good invariance properties, that it is well-suited for recognition tasks, and that it has reasonable generative properties that enable it to denoise video and produce spatio-temporal predictions.
MicroRNAs 15a and 16 regulate tumor proliferation in multiple myeloma (2009)

Detailed genomic studies have shown that cytogenetic abnormalities contribute to multiple myeloma (MM) pathogenesis and disease progression. Nevertheless, little is known about the characteristics of MM at the epigenetic level and specifically how microRNAs regulate MM progression in the context of the bone marrow milieu. Therefore, we performed microRNA expression profiling of bone marrow derived CD138(+) MM cells versus their normal cellular counterparts and validated data by qRT-PCR. We identified a MM-specific microRNA signature characterized by down-expression of microRNA-15a/-16 and overexpression of microRNA-222/-221/-382/-181a/-181b (P < .01). We investigated the functional role of microRNA-15a and -16 and showed that they regulate proliferation and growth of MM cells in vitro and in vivo by inhibiting AKT serine/threonine-protein-kinase (AKT3), ribosomal-protein-S6, MAP-kinases, and NF-kappaB-activator MAP3KIP3. Moreover, miRNA-15a and -16 exerted their anti-MM activity even in the context of the bone marrow milieu in vitro and in vivo. These data indicate that microRNAs play a pivotal role in the biology of MM and represent important targets for novel therapies in MM.