gebhart/patrepeval huggingface dataset:
Detection and characterization of exchangeable protons bound to the hydrogen-activation nickel site of Desulfovibrio gigas hydrogenase: a proton and deuterium Q-band ENDOR study (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetection and characterization of exchangeable protons bound to the hydrogen-activation nickel site of Desulfovibrio gigas hydrogenase: a proton and deuterium Q-band ENDOR studyChaoliang Fan, Miguel Teixeira, Jose Moura, Isabel Moura, Huynh Boi Hanh, Jean Le Gall, Harry D. Peck Jr., and Brian M. HoffmanCite this: J. Am. Chem. Soc. 1991, 113, 1, 20–24Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January 1991https://pubs.acs.org/doi/10.1021/ja00001a003https://doi.org/10.1021/ja00001a003research-articleACS PublicationsRequest reuse permissionsArticle Views257Altmetric-Citations123LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Correction: Role in Diuresis of a Calcitonin Receptor (GPRCAL1) Expressed in a Distal-Proximal Gradient in Renal Organs of the Mosquito Aedes aegypti (L.) (2013)

Evolution of anthropophilic hematophagy in insects resulted in the coordination of various physiological processes for survival.In female mosquitoes, a large blood meal provides proteins for egg production and as a trade-off, rapid elimination of the excess water and solutes (Na + , Cl 2 ) is critical for maintaining homeostasis and removing excess weight to resume flight and avoid predation.This post-prandial excretion is achieved by the concerted action of multiple hormones.Diuresis and natriuresis elicited by the calcitonin-like diuretic hormone 31 (DH 31 ) are believed to be mediated by a yet uncharacterized calcitonin receptor (GPRCAL) in the mosquito Malpighian tubules (MTs), the renal organs.To contribute knowledge on endocrinology of mosquito diuresis we cloned GPRCAL1 from MT cDNA.This receptor is the ortholog of the DH 31 receptor from Drosophila melanogaster that is expressed in principal cells of the fruit fly MT.Immunofluorescence similarly showed AaegGPRCAL1 is present in MT principal cells in A. aegypti, however, exhibiting an overall gradient-like pattern along the tubule novel for a GPCR in insects.Variegated, cell-specific receptor expression revealed a subpopulation of otherwise phenotypically similar principal cells.To investigate the receptor contribution to fluid elimination, RNAi was followed by urine measurement assays.In vitro, MTs from females that underwent AaegGPRcal1 knock-down exhibited up to 57% decrease in the rate of fluid secretion in response to DH 31 .Live females treated with AaegGPRcal1 dsRNA exhibited 30% reduction in fluid excreted after a blood meal.The RNAi-induced phenotype demonstrates the critical contribution of this single secretin-like family B GPCR to fluid excretion in invertebrates and highlights its relevance for the blood feeding adaptation.Our results with the mosquito AaegGPRCAL1 imply that the regulatory function of calcitonin-like receptors for ion and fluid transport in renal organs arose early in evolution.
Age at Menopause in Central Andean Peruvian Women (1997)

The objective of the present study was to determine median age at natural menopause using the status quo method and the sociodemographic and reproductive variables that determine the age at natural menopause in Peruvian women from Lima and Cerro de Pasco. The study was performed in one urban area from Cerro de Pasco (4,340 m above sea level) and in one urban area from Lima (150 m above sea level). The total sample was 469 women aged 28–56 years, 216 from Cerro de Pasco and 253 from Lima. Median age at menopause was 47.1 years at sea level, and 46.14 years at high altitude (p = 0.0005). Results of the proportional hazard regression analysis showed that life at high altitude and lack of use of oral contraceptives were factors associated with early age at menopause, whereas later age at menarche and higher chronological age were associated with delayed age at menopause. In conclusion, altitude is a factor that independently accelerated age at natural menopause.
Integrated Photoelectrochemical Solar Energy Conversion and Organic Redox Flow Battery Devices (2016)

Building on regenerative photoelectrochemical solar cells and emerging electrochemical redox flow batteries (RFBs), more efficient, scalable, compact, and cost-effective hybrid energy conversion and storage devices could be realized. An integrated photoelectrochemical solar energy conversion and electrochemical storage device is developed by integrating regenerative silicon solar cells and 9,10-anthraquinone-2,7-disulfonic acid (AQDS)/1,2-benzoquinone-3,5-disulfonic acid (BQDS) RFBs. The device can be directly charged by solar light without external bias, and discharged like normal RFBs with an energy storage density of 1.15 Wh L<sup>-1</sup> and a solar-to-output electricity efficiency (SOEE) of 1.7 % over many cycles. The concept exploits a previously undeveloped design connecting two major energy technologies and promises a general approach for storing solar energy electrochemically with high theoretical storage capacity and efficiency.
Research on Algae Removal by Ultrasonic and Jet Combined Process (2010)

Ultrasonic and jet combined process was studied to remove algae in lake type raw water. The optimum reaction time was determined by static experiments, and stability of this combined process was checked by dynamic experiments. The results was showed that ultrasonic/jet combined process has excellent removal efficiency on algae in lake type raw water, and ifs a simple operation, and has no secondary pollution. When the pH, temperature, chlorophyll-a (Chl-a) and cell concentration (OD560) of raw water are 7.69, 25.8℃, 29.003 mg/m^3 and 0.091, the average removal efficiency on Chl-a and OD560 are 81.60% and 80.22%, respectively, which are stable by this combined process when it is operating in 36 hours, and samples are analyzed each 4 hours.
Synthesis of Highly Functionalized Amino Acid and Hydroxy Acid Derivatives from γ‐Aminoalkyl‐Substituted α‐Methylene‐γ‐butyrolactones (2002)

Addition of C-, S-, N- and O-nucleophiles to α-amino acid derived α-methylene-γ-butyrolactones provided the corresponding Michael adducts. Addition of cuprates or cyanide, respectively, could be achieved with excellent selectivities. Addition of malonate anion or methoxide could be performed with good yields, but with rather poor selectivities. Addition of N-nucleophiles was achieved with N-benzylhydroxylamine. Further reductive cleavage of the hydroxy and the benzyl groups under hydrogenolytic conditions afforded a diaminocarboxylic acid derivative. Addition of S-nucleophiles furnished the corresponding 1,4 adducts in excellent yields and with good selectivities, especially when a cysteine derivative was used as nucleophile. Hydrogenation of the α-methylene-γ-butyrolactones provided α-methyl-substituted lactones, and ozonolysis yielded the corresponding α,β-dicarbonyl compounds. An isomerization of the exocyclic double bond to an endocyclic double bond was achieved with rhodium chloride as catalyst. The configurations of all products were established by X-ray crystallographic analyses and NMR spectroscopy, including NOE experiments.
ALCOol, vigiLAnce, Charge de travail : facteurs d'accident chez les jeunes conducteurs. Rapport intermédiaire N°1 (2014)

Convention 2013/MP/03 - Fondation Sécurité routière
R o o m A c o u s t i c s (2n d E d.), by Heinrich Kuttruff (1980)

First Page
Analytical calculation of leakage inductance for low-frequency transformer modeling (2013)

In this paper, a new method for the calculation of leakage inductances between short-circuited windings is proposed. The main objective is to find an analytical alternative to the finite element method (FEM) for the computation of short-circuit inductances in electromagnetic transients type (EMT-type) programs. Typical EMT-type tools do not provide complex FEM based computation engines for this sole purpose. The new approach is derived from the method of images and uses analytical formulations for magnetic vector potential and energy developed in this work. It is shown that the difference between the inductance calculated with the FEM in 2-D and that computed with the method of images decreases as the number of layers of images increases. Also, it is demonstrated that the classical approach, based on an axial flux distribution, can lead to considerable error if the windings have unequal heights and if they are located far from the core yokes.
Approximate reconstruction of bandlimited functions for the integrate\n and fire sampler (2009)

In this paper we study the reconstruction of a bandlimited signal from\nsamples generated by the integrate and fire model. This sampler allows us to\ntrade complexity in the reconstruction algorithms for simple hardware\nimplementations, and is specially convenient in situations where the sampling\ndevice is limited in terms of power, area and bandwidth.\n Although perfect reconstruction for this sampler is impossible, we give a\ngeneral approximate reconstruction procedure and bound the corresponding error.\nWe also show the performance of the proposed algorithm through numerical\nsimulations.\n
Self-organization of Patterned CaCO3/Polymer Composite Films: Tuning of Their Morphologies by the Change of Molecular Weights of Acidic Polymers (2006)

Abstract Calcium carbonate (CaCO3) thin films with periodic surface patterns are formed on thin film matrices of a polysaccharide derivative from a solution containing poly(acrylic acid) (PAA). The morphologies of the patterned films have been tuned by the change of molecular weights of the PAA.
Detecting epidemics using highly noisy data (2013)

From Cholera, AIDS/HIV, and Malaria, to rumors and viral video, understanding the causative network behind an epidemic's spread has repeatedly proven critical for managing the spread (controlling or encouraging, as the case may be). Our current approaches to understand and predict epidemics rely on the scarce, but exact/reliable, expert diagnoses. This paper proposes a different way forward: use more readily available but also more noisy data with {\em many false negatives and false positives}, to determine the causative network of an epidemic. Specifically, we consider an epidemic that spreads according to one of two networks. At some point in time we see a small random subsample (perhaps a vanishingly small fraction) of those infected, along with an order-wise similar number of false positives. We derive sufficient conditions for this problem to be detectable, and provide an efficient algorithm that solves the hypothesis testing problem. We apply this model to two settings. In the first setting, we simply want to distinguish between random illness (a complete graph) and an epidemic (spread along a structured graph). In the second, we have a superposition of both of these, and we wish to detect which is the strongest component.
Chitosan/PEI patch releasing EGF and the EGFR gene for the regeneration of the tympanic membrane after perforation (2017)

Damage to the eardrum causes acute pain and can lead to chronic otitis media if it develops into chronic tympanic membrane (TM) perforations. Chronic TM perforations are usually treated with surgical methods such as tympanoplasty and myringoplasty. However, these surgeries are not only complicated and difficult but also cost a lot of money. Our research team developed chitosan patches (E-CPs) that release epidermal growth factor (EGF) as a patch therapy to replace surgical methods. However, there was a limitation in the healing ratio of the treatment compared to the surgical methods. In this study, we developed EGF and epidermal growth factor receptor (EGFR) gene-releasing polyethyleneimine (PEI)/chitosan patches (EErP-CPs) to increase the regeneration of TM perforations. The addition of PEI increased the adhesion and migration ability of TM cells on the patches. The simultaneous release of the EGF and the EGFR gene further enhanced TM cell proliferation, adhesion and migratory ability. It was confirmed that the EGF protein and EGFR gene were released for 30 days; however, EGF was released and increased TM cell viability almost immediately after treatment and EGFR took a minimum of 3 days before showing its effect on improved cell viability. It was also shown that EErP-CPs are more hydrophilic and have more positive charge than E-CP because of added amine groups from PEI. In conclusion, the developed EErP-CPs resulted in the improved healing of TM perforations and can potentially be applied to the regeneration of both chronic and acute tympanic membrane perforations.
Relative Upper Confidence Bound for the K-Armed Dueling Bandit Problem (2013)

This paper proposes a new method for the K-armed dueling bandit problem, a variation on the regular K-armed bandit problem that offers only relative feedback about pairs of arms. Our approach extends the Upper Confidence Bound algorithm to the relative setting by using estimates of the pairwise probabilities to select a promising arm and applying Upper Confidence Bound with the winner as a benchmark. We prove a finite-time regret bound of order O(log t). In addition, our empirical results using real data from an information retrieval application show that it greatly outperforms the state of the art.
A high surface area magnetic photocatalyst with controlled pore size (2009)

Abstract Magnetic photocatalysts have the benefit of recoverability or even confinement/agitation using an oscillating magnetic field. A high surface area magnetic photocatalyst (HSAMP) was developed consisting of silica‐coated barium ferrite and Degussa P25 titanium dioxide dispersed in an acid catalyzed silica xerogel. Through modifying the concentration of hydrofluoric acid catalyst during synthesis, three types of HSAMPs were developed with resulting BET specific surface areas of 374, 543, and 550 m 2 /g. These HSAMPs were assessed for their photocatalytic activity by oxidizing a 6.5 mg/L phenol solution in comparison to Degussa P25 alone. The resulting extent of photocatalysis was greater for the HSAMP than for Degussa P25 although the latter demonstrated a more rapid removal of the phenol parent compound. The HSAMP was capable of removing nearly 100% of the nonpurgeable dissolved organic carbon for 6 repeated uses. Nearly 99% of the HSAMP mass was recoverable with a permanent magnet. © 2009 American Institute of Chemical Engineers Environ Prog, 2010
Phase separation and mechanical properties of polyketone/polyamide polymer alloys (2010)

Abstract We have investigated polyketone (PK)/polyamide 6 (PA) polymer alloys having enhanced Charpy impact energy greater than that of polycarbonate (PC) as a result of moisture absorption. From the results of differential scanning calorimetry (DSC), Raman spectroscopy, and transmission electron microscope (TEM) observation of the polymer alloys, it was found that PK‐rich and PA‐rich phases exist at the nanometer level in the polymer alloys; however, a microscopic interaction phase formed between the two phases. 3D‐TEM observations, electron energy loss spectroscopy, and small‐angle X‐ray scattering measurements revealed that a co‐continuous nanolayer formed from the PA‐rich phase and lamella network of the PK‐rich phase. Moreover, the interaction and mobility of PK and PA molecular chains were investigated by using a 13 C cross polarization/magic angle sample spinning NMR technique. It was found that moisture absorption markedly enhances the mobility of PA molecular chains in PK/PA alloys. This suggests that the wet (moisture‐absorbed) PA phase of PK/PA alloys allows quick deformation upon impact stimulation. On the other hand, the results of Charpy impact tests showed that the total impact energy ( E total ) of the wet polymer alloy was much higher than that of the dry one. An examination of the load‐displacement curves revealed that the wet samples showed a pronounced increase in displacement compared with the dry ones. From these results, it was concluded that the lamella network of the PK‐rich phase sustains the maximum stress and that the large displacement of the PA‐rich phase increases the impact energy. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Author response: Activation of a neural stem cell transcriptional program in parenchymal astrocytes (2020)

Regenerative medicine aims to help the body replace damaged or worn-out tissues, often by kick-starting its own intrinsic repair mechanisms. However, the brain cannot easily repair itself, and therefore poses a much greater challenge. This is because nerve cells or neurons, which underpin learning, memory, and many other abilities, are also the brain’s greatest weakness when it comes to tissue repair. In most parts of the adult brain, neurons are never replaced after they die. This means that damage to brain tissue – for example, after a stroke – can have severe and long-lasting consequences. Neural stem cells are one type of brain cell that can turn into new neurons if needed, but they are only found in a few parts of the brain and cannot fix damage elsewhere. More recent work in mice has shown that astrocytes, a common type of support cell in the brain that help keep neurons healthy, could also generate new neurons following a stroke. However, the ability was restricted to small numbers of astrocytes in a specific part of the brain. Here, Magnusson et al. set out to determine the molecular mechanisms behind this regenerative process and why it is unique to certain astrocytes. The researchers used a technique called single-cell RNA sequencing to analyze the genetic activity within individual mouse astrocytes that had been exposed to conditions mimicking a stroke. This method revealed which genes are switched on or off, thus generating a profile of gene activity for each astrocyte analyzed. This experiment showed that the profiles of astrocytes that had started to produce neurons were in fact nearly identical to neural stem cells. Even the astrocytes that could not generate neurons took the first few steps towards this genetic state; however, they stalled early in the process. Treating the brains of mice withepidermal growth factor, a powerful molecular signal that stimulates cell growth, kick-started nerve cell production in a subset of these cells – showing that at least some of the non-regenerative astrocytes could be stimulated to make neurons if given the right treatment. The results of this study shed new light on how some astrocytes in the brain gain the ability to form new neurons. In the future, this knowledge could help identify a source of replacement cells to regenerate the injured brain.
Transition from the tunneling regime to point contact studied using scanning tunneling microscopy (1987)

Using scanning tunneling microscopy, we have studied local surface modifications induced by point contact of a tunnel tip with metallic surfaces. Two distinct types of topographical modification are found which correlate directly with the chemical condition of the tip as determined by tunneling spectroscopy. Observation of the dynamics of the transition from the tunneling regime to contact permits an evaluation of the gap distance prior to point contact. At gap spacings of s\ensuremath{\lesssim}3 A\r{} a significant decrease in the apparent tunnel barrier height is observed just before touching. Contact was found to initiate with the intimate contact of several atoms only. The implications of these results for lithography on a nanometer scale, and on the investigation of the mechanical properties of surfaces over ranges of a few nanometers are discussed.
Use of Telomerase to Create Bioengineered Tissues (2005)

Telomeres are repetitive DNA (TTAGGG) elements at the ends of chromosomes. Telomerase is a ribonucleoprotein complex that catalyzes the addition of telomeric sequences to the ends of chromosomes. The catalytic protein component of telomerase (hTERT) is expressed only in specific germ line cells, proliferative stem cells of renewal tissues, and cancer cells. The expression of hTERT in normal cells reconstitutes telomerase activity and circumvents the induction of senescence. Telomeres shorten with each cell division, eventually leading to senescence (aging), due to incomplete lagging DNA strand synthesis and end-processing events, and because telomerase activity is not detected in most somatic tissues. There are specific tissues and locations in which replicative senescence likely contributes to the decline in human physiological function with increased age and with chronic illnesses. While expressing hTERT in cells results in the maintenance of telomere length and greatly extended life span, blocking replicative aging systemically would be predicted to increase the potential for tumor formation. However, there are many situations in which the transient rejuvenation of cells could be beneficial. Ectopic expression of hTERT has been shown to immortalize human skin keratinocytes, dermal fibroblasts, muscle satellite (stem), and vascular endothelial, myometrial, retinal-pigmented, and breast epithelial cells. In addition, human bronchial, corneal and skin cells expressing hTERT can be used to form organotypic (3D) cultures (bioengineered tissues) that express differentiation-specific proteins, demonstrating that hTERT by itself does not alter normal physiology. The production of hTERT-engineered tissues offers the possibility of producing tissues to treat a variety of chronic diseases and age-related medical conditions that are due to telomere-based replicative senescence.
Transcriptional Activation of the tad Type IVb Pilus Operon by PypB in Yersinia enterocolitica (2010)

Type IV pili are virulence factors in various bacteria and mediate, among other functions, the colonization of diverse surfaces. Various subclasses of type IV pili have been identified, but information on pilus expression, biogenesis, and the associated phenotypes is sparse for the genus Yersinia. We recently described the identification of PypB as a transcriptional regulator in Yersinia enterocolitica. Here we show that the pypB gene is associated with the tad locus, a genomic island that is widespread among bacterial and archaeal species. The genetic linkage of pypB with the tad locus is conserved throughout the yersiniae but is not found among other bacteria carrying the tad locus. We show that the genes of the tad locus form an operon in Y. enterocolitica that is controlled by PypB and that pypB is part of this operon. The tad genes encode functions necessary for the biogenesis of the Flp subfamily of type IVb pili initially described for Aggregatibacter actinomycetemcomitans to mediate a tight-adherence phenotype. In Y. enterocolitica, the Flp pilin protein shows some peculiarities in its amino acid sequence that imply similarities as well as differences compared to typical motifs found in the Flp subtype of type IVb pili. Flp is expressed and processed after PypB overproduction, resulting in microcolony formation but not in increased adherence to biotic or abiotic surfaces. Our data describe the transcriptional regulation of the tad type IVb pilus operon by PypB in Y. enterocolitica but fail to show most previously described phenotypes associated with this type of pilus in other bacteria.