gebhart/patrepeval huggingface dataset:
Inactivation de biofilms bactériens par jet de plasma froid à la pression atmosphérique (2013)

National audience
Natural polyreactive immunoglobulin A antibodies produced in mouse Peyer’s patches (1999)

To understand the biological function of natural immunoglobulin A (IgA) antibodies in Peyer's patches (PP), we generated IgA monoclonal antibody (mAb) clones from the PP of normal, unimmunized, specific pathogen-free BALB/c mice and examined their reactivities by enzyme-linked immunosorbent assay (ELISA). Many of these antibodies reacted with more than one antigen examined, suggesting that they were polyreactive Abs. Two mAbs agglutinated several different strains of commensal bacteria isolated from mice. To examine the genetic features of these polyreactive mAbs, the VH genes of seven different IgA mAbs were sequenced. The VH genes from the VGAM, J558 and 7183 families were compared with sequence from the mAbs with distinct VDJ rearrangements. One of the mAbs that agglutinated bacteria was encoded by a germline VH gene, but the VH region of the other polyreactive mAbs contained between seven and 11 mutated sites. No indication of antigenic selection was observed in the pattern of these mutated sites. Our results show that polyreactive IgA Abs are present in PP as a part of the normal B-cell repertoire. These polyreactive Abs may establish a natural immune homeostasis, and function as a polyreactive sensor to detect pathogenic invasion and to control immune response in the gut.
Base Damage Immediately Upstream from Double-Strand Break Ends is a More Severe Impediment to Nonhomologous End Joining than Blocked 3′-Termini (2010)

Radiation-induced DNA double-strand breaks (DSBs) are critical cytotoxic lesions that are typically repaired by nonhomologous end joining (NHEJ) in human cells. Our previous work indicated that the highly cytotoxic DSBs formed by (125)I decay possess base damage clustered within 8 to 10 bases of the break and 3'-phosphate (P) and 3'-OH ends. This study examined the effect of such structures on NHEJ in in vitro assays employing either (125)I decay-induced DSB linearized plasmid DNA or structurally defined duplex oligonucleotides. Duplex oligonucleotides that possess either a 3'-P or 3'-phosphoglycolate (PG) or a ligatable 3'-OH end with either an AP site or an 8-oxo-dG 1 nucleotide upstream (-1n) from the 3'-terminus have been examined for reparability. Moderate to severe end-joining inhibition was observed for modified DSB ends or 8-oxo-dG upstream from a 3'-OH end. In contrast, abolition of end joining was observed with duplexes possessing an AP site upstream from a ligatable 3'-OH end or for a lesion combination involving 3'-P plus an upstream 8-oxo-dG. In addition, base mismatches at the -1n position were also strong inhibitors of NHEJ in this system, suggesting that destabilization of the DSB terminus as a result of base loss or improper base pairing may play a role in the inhibitory effects of these structures. Furthermore, we provide data indicating that DSB end joining is likely to occur prior to removal or repair of base lesions proximal to the DSB terminus. Our results show that base damage or base loss near a DSB end may be a severe block to NHEJ and that complex combinations of lesions presented in the context of a DSB may be more inhibitory than the individual lesions alone. In contrast, blocked DSB 3'-ends alone are only modestly inhibitory to NHEJ. Finally, DNA ligase activity is implicated as being responsible for these effects.
Chiral discrimination processes in some metal chelate systems of biological interest (1994)

This thesis reports on the design, synthesis and optical resolution of a set of chiral metal complexes which are intended to be of value for probing the comformational structures of DNA via intercalation.Optimisation of the molecular size of the parent diimine ligand was achieved by synthesis of several phen-based bidentate ligands, including the novel compound dpgq,
Engineering of Nanoscale Heterogeneous Transition Metal Dichalcogenide–Au Interfaces (2023)

Engineering the transition metal dichalcogenide (TMD)-metal interface is critical for the development of two-dimensional semiconductor devices. By directly probing the electronic structures of WS<sub>2</sub>-Au and WSe<sub>2</sub>-Au interfaces with high spatial resolution, we delineate nanoscale heterogeneities in the composite systems that give rise to local Schottky barrier height modulations. Photoelectron spectroscopy reveals large variations (>100 meV) in TMD work function and binding energies for the occupied electronic states. Characterization of the composite systems with electron backscatter diffraction and scanning tunneling microscopy leads us to attribute these heterogeneities to differing crystallite orientations in the Au contact, suggesting an inherent role of the metal microstructure in contact formation. We then leverage our understanding to develop straightforward Au processing techniques to form TMD-Au interfaces with reduced heterogeneity. Our findings illustrate the sensitivity of TMDs' electronic properties to metal contact microstructure and the viability of tuning the interface through contact engineering.
Mortality Among Patients With Chronic Hepatitis B Infection: The Chronic Hepatitis Cohort Study (CHeCS) (2018)

Compared to the general population, CHB patients die at younger ages and higher rates from all causes and liver-related causes. Death certificates underrepresent the true mortality from CHB.
Automatic web query classification using labeled and unlabeled training data (2005)

Accurate topical categorization of user queries allows for increased effectiveness, efficiency, and revenue potential in general-purpose web search systems. Such categorization becomes critical if the system is to return results not just from a general web collection but from topic-specific databases as well. Maintaining sufficient categorization recall is very difficult as web queries are typically short, yielding few features per query. We examine three approaches to topical categorization of general web queries: matching against a list of manually labeled queries, supervised learning of classifiers, and mining of selectional preference rules from large unlabeled query logs. Each approach has its advantages in tackling the web query classification recall problem, and combining the three techniques allows us to classify a substantially larger proportion of queries than any of the individual techniques. We examine the performance of each approach on a real web query stream and show that our combined method accurately classifies 46% of queries, outperforming the recall of the best single approach by nearly 20%, with a 7% improvement in overall effectiveness.
Bayes and Empirical Bayes Methods for Data Analysis (2001)

Approaches for Statistical Inference. The Bayes Approach. The Empirical Bayes Approach. Performance of Bayes Procedures. Bayesian Computation. Model Criticism and Selection. Special Methods and Models. Case Studies. Appendices.
S-100β IS ELEVATED IN THE CEREBROSPINAL FLUID OF CHILDREN WITH MENINGITIS (2002)

SOCIETY OF CRITICAL CARE MEDICINE 32ND CRITICAL CARE CONGRESS SAN ANTONIO, TEXAS, USA JANUARY 28-FEBRUARY 2, 2003: ORAL/SANDWICH PRESENTATIONS: Poster Presentation: Clinical Science: Sepsis Markers: PDF Only
Spray Pyrolysis Synthesis of Mesoporous Lithium-Nickel-Manganese-Oxides for High Energy Li-Ion Batteries (2012)

Uniform, spherical powders consisting of nanostructured Li-excess Li(1+x)Ni0.25Mn0.75O(2.25+x/2) (0.375 ≤ x ≤ 0.5) were synthesized via a spray pyrolysis approach using mixed nitrate precursors. Among the materials produced, the layered Li1.5Ni0.25Mn0.75O2.5 (Rm) attained a capacity of over 250 mAhg−1, which is comparable to that of the Li-excess cathode materials produced via coprecipitation. Li content was varied and found to affect the crystal structure, morphology and electrochemical properties of the materials, and the sintering temperature increased with Li content. Li2MnO3-type structures were observed on XRD diffraction patterns at high annealing temperatures for x < 0.5 due to a phase transformation. The Li2MnO3-type structures degraded the electrochemical performance of these materials due to the high kinetic barrier for Li transportation. In particular, Li1.5Ni0.25Mn0.75O2.5 annealed at 800°C and Li1.375Ni0.25Mn0.75O2.4375 annealed at 700°C predominately adopted a layered composite structure. Nano-domains (5∼10 nm) of spinel structure were observed by HR-TEM to be structurally integrated with the layered structure for Li1.375Ni0.25Mn0.75O2.4375 annealed at 700°C. Nonetheless, the layered Li1.5Ni0.25Mn0.75O2.5 materials showed the best performance in terms of capacity, cycleability and rate-capability among these materials. The superior rate capability of these materials is aided by the nano-structured morphology, which consists of nano-sized primary particles and highly porous secondary particles.
Solvent Accommodation: Functionalities Can Be Tailored Through Co-Crystallization Based on 1:1 Coronene-F4TCNQ Charge-Transfer Complex (2016)

Because organic donor/acceptor blending systems play critical roles in ambipolar transistors, photovoltaics, and light-emitting transistors, it is highly desirable to precisely tailor the stacking of cocrystals toward different intrinsic structures and physical properties. Here, we demonstrated that the structure-stacking modes and electron-transport behaviors of coronene-F4TCNQ cocrystals (1:1) can be tuned through the solvent accommodation. Our results clearly show that the solvent accommodation not only enlarges the inner mixed packing (...DAD···) distances, leading to the depressed short-contact interactions including the side-by-side and face-by-face intermolecular interactions, but also switches off electron-transport behavior of coronene-F<sub>4</sub>TCNQ cocrystals (1:1) in ambient atmosphere.
Models for the pinning of domain walls in Sm(Co,Fe,Cu,Zr)z type magnets at finite temperatures (1999)

We discuss simulation results of three different models for the pinning of the domain walls in permanent magnets as a function of temperature. They correspond to pinning of the wall by periodic arrays of planes, faces of cubes and pyramids. The pinning potential can repel or attract the domain walls. The simulation results are compared with recent experimental data for the 2:17 magnets.
Comparative leaf morpho-anatomy of six species of Eucalyptus cultivated in Brazil (2018)

The present work provides a comparative account of the morpho-anatomy of six species of Eucalyptus, namely E. badjensis Beuzev. & Welch, E. benthamii Maiden & Cambage, E. dunnii Maiden, E. grandis W.Hill, E. globulus Labill. and E. saligna Sm., Myrtaceae. Leaf samples of these six species were investigated by light and scanning electron microscopy. The observed microscopic features that can be useful in the identification and quality control of the studied species include the morphology of epicuticular waxes, presence of prismatic crystals on the leaf surface, leaf midrib shape and arrangement of its vascular system, and the presence or absence of the sclerenchymatous fiber caps in the vascular bundle.
Dual Role of φ29 DNA Polymerase Lys529 in Stabilisation of the DNA Priming-Terminus and the Terminal Protein-Priming Residue at the Polymerisation Site (2013)

Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3' terminal phosphodiester bond. In this paper, site-directed mutants at this Lys residue were used to analyse its functional importance for the synthetic activities of φ29 DNA polymerase, an enzyme that starts linear φ29 DNA replication using a terminal protein (TP) as primer. Our results show that single replacement of φ29 DNA polymerase residue Lys529 by Ala or Glu decreases the stabilisation of the primer-terminus at the polymerisation active site, impairing both the insertion of the incoming nucleotide when DNA and TP are used as primers and the translocation step required for the next incoming nucleotide incorporation. In addition, combination of the DNA polymerase mutants with a TP derivative at residue Glu233, neighbour to the priming residue Ser232, leads us to infer a direct contact between Lys529 and Glu233 for initiation of TP-DNA replication. Altogether, the results are compatible with a sequential binding of φ29 DNA polymerase residue Lys529 with TP and DNA during replication of TP-DNA.
ChemInform Abstract: A Stereoselective Synthesis of cis‐Alkenenitriles Through Reformatskii‐ Peterson Reaction. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Mid-term follow-up after laparoscopic sleeve gastrectomy in obese adolescents. (2014)

LSG is feasible and safe in morbidly obese adolescents, achieving efficient weight loss and impressive resolution of comorbidities. Further studies are required to evaluate the long-term results of this procedure as well as its place among other bariatric options.
On the Green function of the Lord–Shulman thermoelasticity equations (2019)

SUMMARY Thermoelasticity extends the classical elastic theory by coupling the fields of particle displacement and temperature. The classical theory of thermoelasticity, based on a parabolic-type heat-conduction equation, is characteristic of an unphysical behaviour of thermoelastic waves with discontinuities and infinite velocities as a function of frequency. A better physical system of equations incorporates a relaxation term into the heat equation; the equations predict three propagation modes, namely, a fast P wave (E wave), a slow thermal P wave (T wave), and a shear wave (S wave). We formulate a second-order tensor Green's function based on the Fourier transform of the thermodynamic equations. It is the displacement–temperature solution to a point (elastic or heat) source. The snapshots, obtained with the derived second-order tensor Green's function, show that the elastic and thermal P modes are dispersive and lossy, which is confirmed by a plane-wave analysis. These modes have similar characteristics of the fast and slow P waves of poroelasticity. Particularly, the thermal mode is diffusive at low thermal conductivities and becomes wave-like for high thermal conductivities.
Enhanced Boron Tolerance in Plants Mediated by Bidirectional Transport Through Plasma Membrane Intrinsic Proteins (2016)

High boron (B) concentration is toxic to plants that limit plant productivity. Recent studies have shown the involvement of the members of major intrinsic protein (MIP) family in controlling B transport. Here, we have provided experimental evidences showing the bidirectional transport activity of rice OsPIP1;3 and OsPIP2;6. Boron transport ability of OsPIP1;3 and OsPIP2;6 were displayed in yeast HD9 mutant strain (∆fps1∆acr3∆ycf1) as a result of increased B sensitivity, influx and accumulation by OsPIP1;3, and rapid efflux activity by OsPIP2;6. RT-PCR analysis showed strong upregulation of OsPIP1;3 and OsPIP2;6 transcripts in roots by B toxicity. Transgenic Arabidopsis lines overexpressing OsPIP1;3 and OsPIP2;6 exhibited enhanced tolerance to B toxicity. Furthermore, B concentration was significantly increased after 2 and 3 hours of tracer boron ((10)B) treatment. Interestingly, a rapid efflux of (10)B from the roots of the transgenic plants was observed within 1 h of (10)B treatment. Boron tolerance in OsPIP1;3 and OsPIP2;6 lines was inhibited by aquaporin inhibitors, silver nitrate and sodium azide. Our data proved that OsPIP1;3 and OsPIP2;6 are indeed involved in both influx and efflux of boron transport. Manipulation of these PIPs could be highly useful in improving B tolerance in crops grown in high B containing soils.
Simple and Accurate Dependency Parsing Using Bidirectional LSTM Feature Representations (2016)

We present a simple and effective scheme for dependency parsing which is based on bidirectional-LSTMs (BiLSTMs). Each sentence token is associated with a BiLSTM vector representing the token in its sentential context, and feature vectors are constructed by concatenating a few BiLSTM vectors. The BiLSTM is trained jointly with the parser objective, resulting in very effective feature extractors for parsing. We demonstrate the effectiveness of the approach by applying it to a greedy transition-based parser as well as to a globally optimized graph-based parser. The resulting parsers have very simple architectures, and match or surpass the state-of-the-art accuracies on English and Chinese.
Determination of Vitamin K1 Isomers in Foods by Liquid Chromatography with C30 Bonded-Phase Column (2002)

Vitamin K1 was determined in a variety of foods by using reversed-phase liquid chromatography with a C30 column followed by post-column reduction to the fluorescent hydroquinone derivatives. Lipids were removed by lipase digestion, followed by single extraction into hydrocarbon, and the protocol was extended to selected natural and processed foods. Biologically active trans- and inactive ci-vitamin K1 isomers were measured individually to evaluate the true nutritional status of the products. Method performance parameters confirmed the validity of the technique. The use of the triacontyl-bonded C30 phase for selective phylloquinone isomer measurement extends previously validated AOAC Method 999.15 for vitamin K1 in milk and infant formula to a wider range of foods important in the human diet. The cis-vitamin K1 isomer contributes up to about 15% of total phylloquinone in certain foods.
Detection of Flavescence dorée Grapevine Disease Using Unmanned Aerial Vehicle (UAV) Multispectral Imagery (2017)

Flavescence dorée is a grapevine disease affecting European vineyards which has severe economic consequences and containing its spread is therefore considered as a major challenge for viticulture. Flavescence dorée is subject to mandatory pest control including removal of the infected vines and, in this context, automatic detection of Flavescence dorée symptomatic vines by unmanned aerial vehicle (UAV) remote sensing could constitute a key diagnosis instrument for growers. The objective of this paper is to evaluate the feasibility of discriminating the Flavescence dorée symptoms in red and white cultivars from healthy vine vegetation using UAV multispectral imagery. Exhaustive ground truth data and UAV multispectral imagery (visible and near-infrared domain) have been acquired in September 2015 over four selected vineyards in Southwest France. Spectral signatures of healthy and symptomatic plants were studied with a set of 20 variables computed from the UAV images (spectral bands, vegetation indices and biophysical parameters) using univariate and multivariate classification approaches. Best results were achieved with red cultivars (both using univariate and multivariate approaches). For white cultivars, results were not satisfactory either for the univariate or the multivariate. Nevertheless, external accuracy assessment show that despite problems of Flavescence dorée and healthy pixel misclassification, an operational Flavescence dorée mapping technique using UAV-based imagery can still be proposed.
Clinical Features of Celiac Disease Today (1999)

The clinical spectrum of celiac disease has widened over the past decades. The condition is no longer a severe malabsorption syndrome. Instead, a typical celiac disease patient today has merely mild abdominal symptoms. Malabsorption can be subclinical or absent, and there is usually only moderate, if any, loss of weight. Simultaneously, the current prevalence has increased from 1:1,000 to 1:300 inhabitants, or even higher. Clinically silent celiac disease cases are being detected in increasing numbers since the introduction and widespread use of serologic screening tests. Symptoms of celiac disease can appear outside the intestine, a typical example being dermatitis herpetiformis. Gluten intolerance is no longer limited to overt villous atrophy. Inflammation without villous damage may be observed in genetically susceptible individuals. The term latent celiac disease is applied in situations where the patient has normal villous architecture while on a gluten-containing diet, but later develops small bowel villous atrophy compatible with celiac disease.
Lack of Effect of 3,3′5’ -Triiodothyronine (Reverse T3) on Cardiac Function in the Rabbit (1988)

In a recent study, we have shown that the antiarrhythmic drug, amiodarone caused significant elevations of serum rT3 levels and inhibition of the peripheral conversion of T4 to T3 (Kannan et al. Endocrinology 115:1710-1716, 1984). In this study we have examined whether or not rT3 has a direct effect on the monodeiodinase enzyme activity and electropharmacology of the rabbit myocardium. Six male New Zealand White rabbits were administered 50 ug rT3 (a dose about 100 times the daily rT3 production rate) i.p. for 5 days, while the control group received saline. Serum T4, T3 and rT3 concentrations, and myocardial as well as liver and kidney 5'monodeiodinase enzyme activities were measured. Surface ECG and electrophysiological parameters in the atrial, ventricular and sinoatrial node in control and rT3 groups were obtained. Surface ECG did not show any difference in heart rate, RR, PQ, QRS or QT intervals in the two groups. Reverse T3 administration did not produce any significant alterations in APA, MRP, Vmax, APD90 and ERP of atrial and ventricular muscle. Despite administration of a massive dose of rT3, serum levels of T3 and T4 were unchanged as compared to control group. Reverse T3 administration did not produce any alterations in the 5'monodeiodinase activities in the myocardium, liver and kidney. The studies suggest that rT3 does not appreciably affect myocardial function and that the effects of amiodarone on the heart occur via mechanisms other than its ability to cause an increase in serum rT3 levels.
Software package for power system analysis (2014)

Size and complexity of power systems impose significant challenges for the power system analysis and control. As the power system becomes more complex there is a great need to develop improved and sophisticated tools for power system analysis and simulation. These tools should be able to accurately replicate real events that occur in electric power network and help to understand dynamics of changes. A number of software packages have been developed for power system research, analysis, planning, designing and teaching. This paper describes how to utilize an electric Power System Analyzer (PSA) as a useful instrument for power flow studies. The analyzer is designed to be user-friendly, easy-to-use, with the ability to optimize power flow and to visualize the effects of changing problem parameters. PSA can handle power system networks up to 100.000 nodes.