gebhart/patrepeval huggingface dataset:
Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome. (1993)

A 4-kb HindIII fragment that supported the efficient autonomous replication of plasmid vector pDY-, a replication-defective construct based on Epstein-Barr virus sequences, in human K562 cells was rescued from amplified double-minute chromosomes containing the murine adenosine deaminase locus. Polymerase chain reaction assays of size-fractionated nascent strands demonstrated that replication initiation occurred within the same 1- to 2-kb region of this fragment in autonomously replicating plasmids containing the sequence in either orientation, in double-minute chromosomes, and in the single-copy locus at its normal chromosomal location. The complete sequence of this fragment was determined; it contains a 248-bp polypurine tract and consensus binding site sequences for several putative transcription and replication factors.
Differential dynamics and activity-dependent regulation of α- and β-neurexins at developing GABAergic synapses (2010)

Neurexins (NRXs) and neuroligins are key synaptic adhesion molecules that also recruit synaptic signaling machineries. Neurexins consist of α- and β-isoforms, but how they couple synaptic transmission and adhesion to regulate activity-dependent synapse development remains unclear, in part because of poor understanding of their cell biology and regulation in the relevant neurons. Here, we examined the subaxonal localization, dynamics, and regulation of NRX1α and NRX1β in cortical perisomatic inhibitory synapses. Both isoforms are delivered to presynaptic terminals but show significant and different turnover rate at the membrane. Although NRX1α is highly diffuse along developing axons and filopodia, NRX1β is strictly anchored at terminals through binding to postsynaptic ligands. The turnover rate of NRX1β is attenuated by neural activity and presynaptic GABA(B) receptors. NRXs, thus, are intrinsically dynamic but are stabilized by local transmitter release. Such an activity-adjusted adhesion system seems ideally suited to rapidly explore and validate synaptic partners guided by synaptic transmission.
Proteasome regulation of activation-induced T cell death (1997)

Lactacystin, a microbial metabolite that inhibits protease activity only in the proteasome, was used to study the role of the proteasome in the activation-induced cell death (AICD) of T cells. Lactacystin induces DNA fragmentation and apoptosis in a T cell hybridoma (DO.11. 10) in a dose-dependent manner. Between 1 and 10 microM, the mildly cytotoxic lactacystin inhibited the AICD of DO.11.10 cells cultured in anti-CD3-coated wells. Degradation of IkappaBbeta and the translocation of the NF-kappaB (p50/RelA) into the nucleus, which occurred at 1.5 hr after anti-CD3 activation, were inhibited by lactacystin. Lactacystin did not inhibit the expression of nuclear transcription factor Oct-1. The activation-induced expression of the immediate-early gene, Nur77, and the T cell death genes, CD95 (Fas) and CD95 ligand (FasL), were inhibited. Functional expression of FasL cytotoxicity and the increase of cell surface Fas were also inhibited. Lactacystin must be added within 2 hr of activation to efficiently block AICD. In addition, lactacystin failed to inhibit the killing of DO.11.10 by FasL-expressing allo-specific cytotoxic effector cells. These observations strongly suggest a direct link between the proteasome-dependent degradation of IkappaBbeta and the AICD that occurs through activation of the FasL gene and up-regulation of the Fas gene.
Dynamics modelling, simulation, and control of a spacecraft/manipulator system (1987)

Four well-known formalisms for mathematical modelling of the dynamics of a spacecraft/manipulator system are derived from the unifying d'Alembert principle. The Newton-Euler formalism is discussed in more detail, as it allows efficient numerical simulation. An algorithm is defined for the control of the manipulator end-effector motion in inertial space, taking into account the interaction with the spacecraft. Numerical results are presented for the case of a six-degree-of-freedom manipulator mounted on a Hermes-type spacecraft. Paper presented at the First European In-Orbit Operations Technology Symposium, Darmstadt, West Germany, 7-9 September 1987.
Access Security in UMTS and IMS (2011)

Motivated by the requirements for higher data rate, richer multimedia services, and broader radio range wireless mobile networks are currently in the stage evolving from the second-generation (2G), for example, global system for mobile communications (GSM), into the era of third-generation (3G) or beyond 3G or fourth-generation (4G). Universal mobile telecommunications system (UMTS) is the natural successor of the current popular GSM (http://www.3gpp.org) code division multiple access 2000 (CDMA2000) is the next generation version for the CDMA-95, which is predominantly deployed in North America and North Korea. Time division-sychrononous CDMA (TD-SCDMA) is in the framework of 3rd generation partnership project 2 (3GPP2) and is expected to be one of the principle wireless technologies employed in China in the future (http://www.3gpp.org; 3G TS 35.206). It is envisioned that each of three standards in the framework of international mobile telecommunications- 2000 (IMT-2000) will play a significant role in the future due to the backward compatibility, investment, maintenance cost, and even politics.Request access from your librarian to read this chapter's full text.
PROBLEMAS Y RETOS DE LA INVESTIGACION EDUCATIVA EN EL SIGLO XXI. EL CASO DE LA RIEMS Y LA CONFORMACION ANTROPOETICA (2010)

En este documento abordamos la problemática que representa la Reforma Integral de la Educación Media Superior (RIEMS)1 en México, no sólo para la investigación, sino también para el proceso de conformación de la antropoética. Ya que la investigación en el siglo XXI enfrenta un sin número de retos y problemas, en esta centuria en que vivimos, el reto no sólo de la investigación si no de la educación, es que debe conciliarse la materia y el espíritu, la naturaleza y la cultura, la ciencia y las humanidades, propiciando un reencuentro entre la tecnología y el humanismo, integrando la memoria y el proyecto. Por ello creemos que el nuevo paradigma de la investigación en educación es la conformación de la antropoética. En este sentido se abordan sus términos conceptuales, como antropoética, RIEMS, competencias transversales, así como, las nociones de conformación y complejidad. Hemos seleccionado para ello el escenario de la educación media superior, específicamente el bachillerato, porque es en este nivel de la educación, donde se generan diversas modalidades educativas que derivan en prácticas neoliberalistas de capacitación e instrucción, tal es el caso de la RIEMS, lo que inhibe el proceso de formación humana y por ende su posibilidad de formación antropoética. Esta última como una opción clave para la educación de las futuras generaciones.
The need for flexibility in dosing of hypnotic agents. (2000)

Although a variety of medical and psychiatric disorders are known to cause insomnia, there are many patients for which a cause cannot be determined. When the etiology is unknown, treatment of insomnia must be symptomatic. Epidemiologic studies suggest that insomnia does not occur on a regular basis in most people. In addition, the presentation of insomnia relative to the time of night is often variable, with waking in the middle of the night and initiating sleep upon going to bed being the most common. The intermittent occurrence of most insomnia suggests that treatment is best accomplished by using hypnotics on an "as needed" basis when difficulties with sleep occur. When pharmacological treatment of insomnia is warranted, benzodiazepine receptor agonists (BzRAs) are often the preferred class of agents. Agents with a shorter duration of action and rapid onset of action are preferred for flexible administration, providing an option for middle of the night dosing if this is when insomnia occurs. Of the available hypnotic agents in the BzRA class, triazolam, zolpidem, and zaleplon have rapid onsets of action and short half-lives. However, with a half-life of 1 hour, only zaleplon appears to be suited for middle of the night administration. Other important factors that affect selection of an agent for the treatment of intermittent insomnia include psychomotor or cognitive impairment and rebound insomnia after discontinuation of therapy. In one placebo-controlled trial, residual sedation was seen after flurazepam, but not with zaleplon, following middle-of-the-night administration. In addition, rebound insomnia was not apparent in a 4-week, placebo-controlled trial of zaleplon. In this same study, transient rebound insomnia was apparent with zolpidem compared to placebo. More data are needed on long-term therapy with hypnotic agents given intermittently on nights during which insomnia occurs.
Supramolecular Pattern of Fullerene on 2D Bimolecular “Chessboard” Consisting of Bottom-up Assembly of Porphyrin and Phthalocyanine Molecules (2007)

Two-component adlayers consisting of zinc(II) phthalocyanine (ZnPc) and a metalloporphyrin, such as zinc(II) octaethylporphyrin (ZnOEP) or zinc(II) tetraphenylporphyrin (ZnTPP), were prepared by immersing either an Au(111) or Au(100) substrate in a benzene solution containing those molecules. The bimolecular adlayers thus prepared were investigated in 0.1 M HClO4 by cyclic voltammetry (CV) and electrochemical scanning tunneling microscopy (EC-STM). A supramolecularly organized "chessboard" structure was formed for the ZnPc and ZnOEP bimolecular array on Au(111), while characteristic nanohexagons were found in the ZnTPP and ZnOEP bimolecular adlayer. EC-STM revealed that the surface mobility and the molecular re-organization of ZnPc and ZnOEP on Au(111) were tunable by manipulating the electrode potential, whereas the ZnTPP and ZnOEP bimolecular array was independent of the electrode potential. A "bottom-up" hybrid assembly of fullerene molecules was formed successfully on an alternate array of bimolecular ZnPc and ZnOEP molecules. The bimolecular "chessboard" served as a template to form the supramolecular assembly of C60 by selective trapping in the open spaces. A supramolecular organization of ZnPc and ZnOEP was also found on the reconstructed Au(100)-(hex) surface. A highly ordered, compositionally disordered but alternate array of ZnPc and ZnOEP was formed on the reconstructed Au(100)-(hex) surface, indicating that the bimolecular adlayer structure is dependent on the atomic arrangement of underlying Au in the formation of supramolecular nanostructures composed of those molecules. On the bimolecular array consisting of ZnPc and ZnOEP on the Au(100)-(hex), no highly ordered supramolecular assembly of C60 was found, suggesting that the supramolecular assembly of C60 molecules is strongly dependent upon the bimolecular packing arrangement of ZnPc and ZnOEP.
Abstract 3214: FePt nanoparticles overcoming radiotherapy resistance in hCtr-1 high expression cells via mitochondria targeting (2018)

Abstract The glutathione (GSH) overexpression small-cell-lung-cancer cell lines, SR3A-13 and SR3A-14, transfected with recombinant DNA encoding γ-glutamylcysteine synthetase (γ-GCSh), are radiation resistant. However, they are more sensitive to cisplatin treatment, compared with parental cell (SR3A)1. In addition to be a copper transporter, the hCtr-1 is also a transporter for platinum (Pt)-based agents. Therefore, the cellular uptake of cisplatin is enhanced by the upregulation of human copper transporter 1 (hCtr-1) at the cell membrane. We have synthesized an alloy FePt nanoparticles (FePt NPs) that generated excellent contrast effects in both CT and MRI images2. Interestingly, the uptake of FePt NPs by SR3A-13 and -14 cells was 3-3.5 fold higher than that of SR3A cells, measured with inductively coupled plasma optical emission spectrometry (ICP-OES) and transmission electron microscopy (TEM). Because intracellular NPs have been shown to generate reactive oxygen species (ROS) and overproduction of ROS may enhance the efficacy of radiotherapy, we sought to explore whether treatment with the alloy FePt NPs can reverse the radiation resistance in SR3A-13 and -14 cells. We found co-treatment with the alloy FePt NPs and radiation effectively reduce the survivals of these 2 cells. Furthermore, we found an alternation in mitochondrial appearance accompanied with low signal of Tetramethylrhodamine, methyl ester (TMRM), indicating a malfunction is induced in mitochondria. We conclude that FePt NPs are able to overcome radiation resistance in hCtr-1 high expression cells via mitochondria targeting. Citation Format: Tsung-Lin Tsai, Macus Tien Kuo, Jen-Ya Wang, Wan-Li Shin, Helen H. W. Chen, Wu-Chou Su. FePt nanoparticles overcoming radiotherapy resistance in hCtr-1 high expression cells via mitochondria targeting [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3214.
Loss Separation in Nonoriented Electrical Steels (2010)

The accuracy of conventional loss separation technique as applied to nonoriented electrical steel is evaluated. It is shown that the main reason of inaccuracy of the loss separation carried out analytically is the use of simplified equation for the classical eddy-current loss originated from the theory developed for a linear magnetic media. The error of the order of 10% in classical loss at 1.5 T results in errors of 25 and 50% in excess loss at 50 and 100 Hz, respectively.
Laboratory astrophysics: Measurements of n=nʹ to n=2 line emission in Fe16+ to Fe23+ (1996)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation D. W. Savin, P. Beiersdorfer, G. V. Brown, J. Crespo López-Urrutia, V. Decaux, S. M. Kahn, D. A. Liedahl, K. J. Reed, K. Widmann; Laboratory astrophysics: Measurements of n=n′ to n=2 line emission in Fe16+ to Fe23+. AIP Conf. Proc. 15 July 1996; 381 (1): 39–46. https://doi.org/10.1063/1.51341 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
CD4+FoxP3+ regulatory T cells confer infectious tolerance in a TGF‐β dependent manner (2008)

CD4 + FoxP3 + regulatory T cells (T R ) comprise a separate lineage of T cells that are essential for maintaining immunological tolerance to self. One molecule that has been extensively studied in T R suppression is TGF‐β, but its importance remains controversial. To further analyze the contribution of TGF‐β in T R function, we have used recombinant TGF‐β latency associated peptide (LAP) to neutralize active TGF‐β. LAP modestly reversed the non‐responsive state of CD4 + CD25 + T cells from conventional mice, but had no effect on Foxp3+ T R from Foxp3‐GFP knock in mice, suggesting that T R associated TGF‐β plays a role in suppressing the responses of activated T cells. Co‐culture of pre‐activated Foxp3 + T R and naïve Foxp3 − CD4+ T cells in the presence of anti‐CD3 and IL‐2 results in the induction of Foxp3 expression in 10–20% of the responder cells. This conversion is dependent on TGF‐β as it can be blocked with LAP and requires contact between the T R and effector T cell. The induced CD4 + FoxP3 + T cells are suppressive when co‐cultured with fresh CD4 + Foxp3 − T cells in vitro and when mixed with CD4 + Foxp3 − T cells in vivo . Thus, it appears that T R associated TGF‐β can play a role in suppression of activated effectors, but more importantly can mediate infectious tolerance by converting conventional T cells into T R .
High-Isolation Topology for Filtering Power Dividers Based on Complex Isolation Impedance and Surface Wave Suppression (2020)

Aiming at high and wideband output isolation, a new topology of filtering power divider based on complex isolation impedance and surface wave mitigation techniques is proposed. Different from previously published filtering power dividers using pure resistor, an analytical design method by using complex impedance is proposed in this article. To formula the complex impedance, the LC equivalent circuit model is introduced, and the closed-form derivation is given accordingly. Based on the calculated impedance, high isolation can be theoretically realized in circuit level. However, the output isolation is limited below 20 dB in practical EM simulations. To solve this issue, the filtering power divider is further investigated in field level. To reduce the crosstalk between adjacent filtering channels, the surface wave mitigation techniques are investigated. Accordingly, a 16-dB enhancement of output isolation is realized, without sacrificing other filtering specifications. For experimental verification, a filtering power divider is designed, fabricated, and measured. A significant size reduction and 36-dB output isolation with over 500% isolation bandwidth can be observed, verifying the validity of the proposed design method.
Degradation of Phytate by the 6-Phytase from Hafnia alvei: A Combined Structural and Solution Study (2013)

Phytases hydrolyse phytate (myo-inositol hexakisphosphate), the principal form of phosphate stored in plant seeds to produce phosphate and lower phosphorylated myo-inositols. They are used extensively in the feed industry, and have been characterised biochemically and structurally with a number of structures in the PDB. They are divided into four distinct families: histidine acid phosphatases (HAP), β-propeller phytases, cysteine phosphatases and purple acid phosphatases and also split into three enzyme classes, the 3-, 5- and 6-phytases, depending on the position of the first phosphate in the inositol ring to be removed. We report identification, cloning, purification and 3D structures of 6-phytases from two bacteria, Hafnia alvei and Yersinia kristensenii, together with their pH optima, thermal stability, and degradation profiles for phytate. An important result is the structure of the H. alvei enzyme in complex with the substrate analogue myo-inositol hexakissulphate. In contrast to the only previous structure of a ligand-bound 6-phytase, where the 3-phosphate was unexpectedly in the catalytic site, in the H. alvei complex the expected scissile 6-phosphate (sulphate in the inhibitor) is placed in the catalytic site.
rAAV-mediated overexpression of TGF-β stably restructures human osteoarthritic articular cartilage in situ (2013)

The present findings show the ability of rAAV-mediated TGF-β gene transfer to directly remodel human OA cartilage by activating the biological, reparative activities and by regulating hypertrophy and terminal differentiation in damaged chondrocytes as a potential treatment for OA or for other disorders of the cartilage that may require transplantation of engineered cells.
Enhanced Capacity and Cyclability of Si@NiSi2 Nanocomposite Anodes Fabricated by Facile Electroless Ni Plating (2022)

Although silicon (Si) has been proposed as the most promising anode material in lithium-ion batteries due to their high capacity (∼4000 mA h g–1), the poor cyclability resulting from the strain-induced pulverization caused by the severe volume change during charge/discharge hinders the industrial applications. Here, Si nanoparticles were conformally coated with either Ni, NiO, or NiSi2 to suppress the tremendous volume change of Si, hence leading to prolonging the cycle life of the Si anode to a different degree. Both Si@Ni and Si@NiO nanocomposites were synthesized by one-step self-reducing (SR) electroless nickel plating without/with heat treatment, respectively, whereas Si@NiSi2 nanocomposites were obtained by a two-step (SR + electroless nickel deposition, EN) process, followed by heat treatment. Among all these three nanocomposite anodes, Si@NiSi2 prepared by 5 min of SR, 30 min of EN, and 400 °C annealing in sequence achieved the highest capacity of ∼1390 mA h g–1 and the best capacity retention of ∼86.6% with a Coulombic efficiency of 99% over 70 cycles. For comparison, the capacity retention of the Si@Ni and Si@NiO electrode over 70 cycles is estimated to be ∼67 and 75%, respectively. These results suggest that NiSi2 could be a promising protective coating layer to effectively buffer the volume change of Si and promote the battery life.
In search of rat stem Leydig cells: Identification, isolation, and lineage-specific development (2006)

Leydig cells (LCs) are thought to differentiate from spindle-shaped precursor cells that exhibit some aspects of differentiated function, including 3beta-hydroxysteroid dehydrogenase (3betaHSD) activity. The precursor cells ultimately derive from undifferentiated stem LCs (SLCs), which are postulated to be present in testes before the onset of precursor cell differentiation. We searched for cells in the neonatal rat testis with the abilities to: (i) proliferate and expand indefinitely in vitro (self renew); (ii) differentiate (i.e., 3betaHSD and ultimately synthesize testosterone); and (iii) when transplanted into host rat testes, colonize the interstitium and subsequently differentiate in vivo. At 1 week postpartum, spindle-shaped cells were seen in the testicular interstitium that differed from the precursor cells in that they were 3betaHSD-negative, luteinizing hormone (LH) receptor (LHR)-negative, and platelet-derived growth factor receptor alpha (PDGFR alpha)-positive. These cells were purified from the testes of 1-week-old rats. The cells contained proteins known to be involved in LC development, including GATA4, c-kit receptor, and leukemia inhibitory factor receptor. The putative SLCs expanded over the course of 6 months while remaining undifferentiated. When treated in media that contained thyroid hormone, insulin-like growth factor I, and LH, 40% of the putative SLCs came to express 3betaHSD and to synthesize testosterone. When transplanted into host rat testes from which LCs had been eliminated, the putative SLCs colonized the interstitium and subsequently expressed 3betaHSD, demonstrating their ability to differentiate in vivo. We conclude that these cells are likely to be the sought-after SLCs.
A promoter mutation in the haemagglutinin segment of influenza A virus generates an effective candidate live attenuated vaccine (2014)

We demonstrate that NIBRG-93 is an effective live attenuated vaccine virus protecting mice against lethal challenge and reducing virus shedding.
Detecting data store access conflict in Simulink by solving Boolean satisfiability problems (2010)

This paper presents a method to statically analyze a Simulink <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">®</sup> model to detect two potential problems with data store memory blocks: (i) a value may be read from a data store before it is written and (ii) a data store may be overwritten before its value is read by other blocks. The analysis employs a Boolean satisfiability (SAT) solver and so obviates extensive testing by means of simulation. It is illustrated how this supports model elaboration in Model-Based Design by performing the analysis on a task model of a digital controller implementation.