gebhart/patrepeval huggingface dataset:
Rapid cell-patterning and microfluidic chip fabrication by crack-free CO2laser ablation on glass (2006)

This paper uses a widely available CO2 laser scriber (λ = 10.6 µm) to perform the direct-writing ablation of quartz, borofloat and pyrex substrates for the development of microfluidic chips and cell chips. The surface quality of the ablated microchannels and the presence of debris and distortion are examined by scanning electron microscopy, atomic force microscopy and surface profile measurement techniques. The developed laser ablation system provides a versatile and economic approach for the fabrication of glass microfluidic chips with crack-free structures. In the laser writing process, the desired microfluidic patterns are designed using commercial computer software and are then transferred to the laser scriber to ablate the trenches. This process eliminates the requirement for corrosive chemicals and photomasks, and hence the overall microchip development time is limited to less than 24 h. Additionally, since the laser writing process is not limited by the dimensions of a photomask, the microchannels can be written over a large substrate area. The machining capability and versatility of the laser writing system are demonstrated through its application to the fabrication of a borofloat microfluidic chip and the writing of a series of asymmetric trenches in a microwell array. It is shown that the minimum attainable trench width is 95 µm and that the maximum trench depth is 225 µm. The system provides an economic and powerful means of rapid glass microfluidic chip development. A rapid cell-patterning method based on this method is also demonstrated.
A simplified method for culture of oral epithelial cells (1985)

In order to facilitate studies on oral mucosa a simplified method for the culture of oral epithelial cells from adult hamsters was developed. Cheek pouches were excised and epithelial cells isolated by collagenase digestion. These were grown in CM-V medium containing spermine in order to inhibit overgrowth of the epithelial cells by fibroblasts. The epithelial cells were subcultured by routine tissue culture procedures. The cells isolated were examined by light microscopy and scanning and transmission electron microscopy. Morphologically the cells were typical of epithelial cells. Ultrastructural examination showed structures typical of epithelia including filaments, keratohyalin granules and desmosomal junctions. The culture system provides epithelial cells that can be used for a variety of biochemical and morphological studies.
Neutron scattering investigation of the spin-flop transition in MnCl2·4D2O (1981)

A detailed study of the spin-flop transition in Mn${\mathrm{Cl}}_{2}$\ifmmode\cdot\else\textperiodcentered\fi{}4${\mathrm{D}}_{2}$O has been carried out. The characteristics of the first-order "shelf," as determined by neutron scattering, were found to be in good agreement with the phase diagram derived here for the appropriate monoclinic symmetry. Experimental and theoretical analysis of the critical scattering observed at the spin-flop transition close to the bicritical point provide independent confirmation of the first-order nature of the transition.
Spectroscopic studies of a prototype electrically pumped COIL system (2004)

This paper discusses methods, using non-intrusive diagnostic techniques, to characterize the detailed dynamics of I* gain and O<sub>2</sub>(a<sup>1</sup>&Delta;) yield on a laboratory microwave-discharge flow reactor, for conditions relevant to the electrically driven COIL concept. The key diagnostics include tunable diode laser absorption measurements of I* small-signal gain and temperature, high-precision absorption measurements of reactor I<sub>2</sub> concentrations, absolute and relative spectral emission measurements of O<sub>2</sub>(a<sup>1</sup>&Delta;) and I* concentrations, and air-afterglgow determinations of O concentrations. We have characterized variations in O and O<sub>2</sub>(a) yields with discharge power and oxygen mole fraction. We observe O<sub>2</sub>(a) yields to increase dramatically with decreasing oxygen mole fraction. We have also demonstrated a spectral fitting analysis technique capable of quantifying the presence of vibrationally excited O<sub>2</sub>(a,v). This combined suite of diagnostics offers a comprehensive approach to performance characterization for electrically driven COIL concepts.
Impaired Temporal Discrimination in Parkinson's Disease: Temporal Processing of Brief Durations as an Indicator of Degeneration of Dopaminergic Neurons in the Basal Ganglia (1997)

Recent findings suggest that temporal processing of brief durations is a function of dopaminergic neurotransmission in the basal ganglia. Furthermore, there is preliminary evidence of abnormal timing functions in patients suffering from idiopathic Parkinson's disease (PD). In the present study, temporal discrimination of intervals in the range of milliseconds was investigated in 20 PD patients and 20 healthy controls matched for sex and age. Temporal discrimination was significantly impaired in PD patients as compared to healthy controls. For PD patients, additional correlational analyses did not yield any significant relationship between performance on temporal processing and degree of motor impairment or illness duration. However, impairment in temporal processing was associated with dosage of L-dopa substitution and self-rated feelings of depression. The overall pattern of results suggests that deficits in temporal information processing observed in PD patients may represent a trait marker of vulnerability to decreasing levels of dopaminergic activity in the basal ganglia rather than a state-dependent indicator of the acute clinical symptomatology.
In vivo recombination of Saccharomyces eubayanus maltose-transporter genes yields a chimeric transporter that enables maltotriose fermentation (2018)

Abstract Saccharomyces pastorianus lager-brewing yeasts are aneuploid S. cerevisiae x S. eubayanus hybrids, whose genomes have been shaped by domestication in brewing-related contexts. In contrast to most S. cerevisiae and S. pastorianus strains, S. eubayanus cannot utilize maltotriose, a major carbohydrate in brewer’s wort. Accordingly, S. eubayanus CBS 12357 ⊤ harbors four SeMALT maltose-transporter genes, but no genes resembling the S. cerevisiae maltotriose-transporter gene ScAGT1 or the S. pastorianus maltotriose-transporter gene SpMTY1 . To study the evolvability of maltotriose utilization in S. eubayanus CBS 12357 ⊤ , maltotriose-assimilating mutants obtained after UV mutagenesis were subjected to laboratory evolution in carbon-limited chemostat cultures on maltotriose-enriched wort. An evolved strain showed improved maltose and maltotriose fermentation, as well as an improved flavor profile, in 7-L fermenter experiments on industrial wort. Whole-genome sequencing revealed a novel mosaic SeMALT413 gene, resulting from repeated gene introgressions by non-reciprocal translocation of at least three SeMALT genes. The predicted tertiary structure of S e Malt413 was comparable to the original S e Malt transporters, but overexpression of SeMALT413 sufficed to enable growth on maltotriose, indicating gene neofunctionalization had occurred. The mosaic structure of SeMALT413 resembles the structure of S. pastorianus maltotriose-transporter gene SpMTY1 , which has sequences with high similarity to alternatingly ScMALx1 and SeMALT3 . Evolution of the maltotriose-transporter landscape in hybrid S. pastorianus lager-brewing strains is therefore likely to have involved mechanisms similar to those observed in the present study. Author Summary Fermentation of the wort sugar maltotriose is critical for the flavor profile obtained during beer brewing. The recently discovered yeast Saccharomyces eubayanus is gaining popularity as an alternative to S. pastorianus and S. cerevisiae for brewing, however it is unable to utilize maltotriose. Here, a combination of non-GMO mutagenesis and laboratory evolution of the S. eubayanus type strain CBS 12357 ⊤ was used to enable maltotriose fermentation in brewer’s wort. A resulting S. eubayanus strain showed a significantly improved brewing performance, including improved maltose and maltotriose consumption and a superior flavor profile. Whole genome sequencing identified a novel transporter gene, SeMALT413 , which was formed by recombination between three different SeMALT maltose-transporter genes. Overexpression of SeMALT413 in CBS 12357 ⊤ confirmed its neofunctionalization as a maltotriose transporter. The mosaic structure of the maltotriose transporter SpMty1 in S. pastorianus resembles that of S e Malt413, suggesting that maltotriose utilization likely emerged through similar recombination events during the domestication of current lager brewing strains.
New quantitative high-performance liquid chromatography method for analysis of gramine in cereal leaf tissue (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew quantitative high-performance liquid chromatography method for analysis of gramine in cereal leaf tissueAlister D. Muir, Neil D. Westcott, and Christopher F. HinksCite this: J. Agric. Food Chem. 1992, 40, 10, 1836–1838Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/jf00022a021https://doi.org/10.1021/jf00022a021research-articleACS PublicationsRequest reuse permissionsArticle Views135Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A small-signal equivalent-circuit model for GaAs-AlxGa1−xAs resonant tunneling heterostructures at microwave frequencies (1987)

This paper presents a study of the microwave impedance of GaAs-AlxGa1−xAs resonant tunneling heterostructures. An equivalent-circuit model is proposed that accounts for the frequency variation of the measured impedance and whose elements correspond to physical phenomena believed to be present in the device. Empirical formulas are obtained which can be used to calculate the values of the equivalent-circuit elements from the structural parameters and the dc current-voltage characteristics of the device.
<title>Laser-induced ablation and condensation on a close-to-target substrate</title> (1997)

Ablation and condensation of film target under conditions of close-to-target substrate are considered. Different mechanisms of before-threshold film tearing-off from the donor substrate are examined. There is investigated the evaporative regime of the front transfer; thermal, gas- dynamical and hydrodynamical aspects of the process are considered. Investigations of the back transfer showed limitation of the deposited layer thickness in the multipulse regime. The maximum condensate thickness is defined.
Magnetic and structural properties of Rh substituted Co-Cr alloy films with perpendicular magnetic anisotropy (1981)

RF sputtered Co0.78−xCr0.22Rhx (where x is 0–0.06) alloy films for high density perpendicular recording were studied. First, Co-M (M = Cr, Ti, V, Mo, Pd, and Rh) alloy films were studied. They exhibit hcp crystallization and have a high orientation of the c-axis normal to the film plane. However, they have a smaller coercive force (less than 300 Oe), and with the exception of Co-Cr, they do not have a columnar structure or satisfy the relation Hk ≳4πMs. Next, Co-Cr-Rh alloy films were studied. They also exhibit hcp crystallization and their structure is columnar. They show perpendicular magnetic anisotropy similar to that of Co-Cr, and in particular, a higher c-axis orientation was obtained. The ΔΘ50, half the width of a rocking curve of a (002) peak, is about 20–40% smaller than that of Co-Cr. By measuring the recording characteristics of Co-Cr-Rh disks (on an anodized aluminum substrate), a maximum recording density of 23,000FRPI was obtained.
Impairment of Airway Mucociliary Transport by Pseudomonas aeruginosa Products: Role of Oxygen Radicals (1992)

We previously showed that the supernatant of a Pseudomonas aeruginosa (PA) culture and its constituents pyocyanin and 1-hydroxyphenazine inhibit ciliary activity of dispersed tracheal epithelial cells in vitro via the generation of oxygen radicals by phagocytes. In the present study, we wished to determine if tracheal mucus velocity (TMV) is also impaired by PA supernatant and if oxygen radicals have a mediating role. In conscious sheep, TMV (measured with a radiographic method) was determined before and serially following aerosol challenge with the cell-free supernatant of a PA culture or unconditioned culture medium (control). TMV decreased from a mean (+/- SEM) baseline of 6.7 +/- 1.1 mm/min (n = 6) by 29, 35, and 25% at 0.5, 3, and 24 h after challenge, respectively (p < 0.05), and returned to baseline 1 wk later (-6%, p = NS). Control medium had no effect on TMV (maximum decrease by 15% at 0.5 h). Aerosolized catalase blunted the effect of PA supernatant on TMV. To determine if the impairment of TMV involved ciliary inhibition, tissues were mounted in a chamber and ciliary beat frequency (CBF) and surface liquid velocity (SLV) were measured with a microscopic method. PA supernatant decreased both CBF (maximum mean decrease 12%; n = 5, p < 0.05) and SLV (maximum mean decrease 78%; n = 5, p < 0.05) in a dose-dependent fashion, with a correlation between the two parameters; these effects were blocked by catalase.(ABSTRACT TRUNCATED AT 250 WORDS)
Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis (2009)

MicroRNAs (miRNAs) are comprised of approximately 21 nucleotide (nt) RNAs that play important regulatory roles in growth and development by targeting mRNAs for cleavage or translational repression. In Arabidopsis, more than one hundred miRNAs have been identified but the biological functions of only a limited number of them have been determined by molecular genetic analysis. miR396 is a miRNA conserved among the dicot and monocot plants. In Arabidopsis, miR396 has two loci (MIR396a and MIR396b) and targets six Growth-Regulating Factor (GRF) genes encoding putative transcription factors with roles in plant leaf growth. Using a northern blot hybridizations approach, we have found that MIR396 is predominantly expressed in leaf and seedling. To further analyze the role of miR396 in the regulation of target genes and leaf growth, we have generated transgenic Arabidopsis plants that constitutively overexpress MIR396a or MIR396b. These transgenic plants have narrow-leaf phenotypes due to reduction in cell number. Ectopic overexpression of MIR396 represses expression of not only six GRF genes but also GIF1 encoding a GRF-interacting transcription coactivator with a role in cell proliferation in leaf. In addition, transgenic MIR396-overexpressing plants have lower densities of stomata and are more tolerant to drought than wild-type plants. These results strongly support the belief that miR396 plays an important role in plant leaf growth and development, most likely by repressing GRF gene expression.
Clinical Experience with the Elema Vario Pacemaker (1974)

Early problems after implantation of an endocardial electrode for permanent pacing occur in many patients. Difficulties can be anticipated in many of these patients and their management made much easier by a knowledge of the daily endocardial threshold obtained in patients with an implanted Elema Verio pacing unit by a simple non-invasive technique using a magnet and electrocardiogram. Twenty patients are described in whom permanent pacing with an Elema Vario unit was undertaken. The practical advantages of this unit can be readily seen in the patient who developes exit block or in the patient with obstructive airways disease, in whom frequent coughing or right ventricular hypertrophy may make stable electrode placement difficult. The chief advantage of the Elema Vario pacemaker is the extreme simplicity with which knowledge of the endocardial threshold can be obtained.
A 6.1-mW dual-loop digital DLL with 4.6-ps RMS jitter using window-based phase detector (2007)

A 0.13-µm CMOS dual-loop digital DLL for multiphase clock generation and synchronization is presented. Ten clock phases are produced and locked to a first reference clock by the inner loop, while the outer loop further aligns all phases simultaneously to a second reference clock at a higher frequency. Unlike usual dual-loop DLLs, the proposed architecture has one loop completely enclosed inside the other, resulting in a secondorder behavior. A window-based phase detection technique is exploited to minimize the circuit complexity and power with uncompromised jitter performance. The false-lock-free DLL operates over a wide frequency range of 0.2-1.2 GHz, measures a 4.6-ps rms jitter, and consumes 6.1 mW at 1.2 GHz.
CT-geleide puncties en interventies (2018)

Met behulp van CT kunnen accurate puncties en drainages worden verricht op geleide van beeldvorming. Door gebruik te maken van een stapsgewijze benadering, waarbij telkens de positie kan worden gecontroleerd door vervaardigen van CT-coupes, kan een bepaalde locatie zeer gericht worden benaderd en bereikt. Met de komst van de snelle multi-slice-CT is de hoeveelheid CT-geleide interventies alleen maar toegenomen.
Purification of Toxoplasma gondii suspensions from mice peritoneal exudate. (1982)

Purification procedures of Toxoplasma gondii suspensions are described. These methods were designed for the elimination of leucocytes or TG 180 cells. We have been using either gradient centrifugation in Ficoll or gel filtration. In the first case, only leucocytes are eliminated (95% of the initial content). On the other hand, gel filtration allowed the elimination of TG 180 cells. The yield (67% to 98%) depends on the chosen gel (Ultrogel AcA 202 or Sephadex G25 fine), and on the amount of TG 180 cells present before filtration.
Vitreous VEGF Clearance Is Increased after Vitrectomy (2010)

Purpose. Pars plana vitrectomy (PPV) has been reported to reduce macular thickness and improve visual acuity in patients with diabetic macular edema (ME). The hypothesis for the study was that after PPV, clearance is accelerated and VEGF concentrations are reduced. To test this hypothesis, hVEGF(165) injections were performed in rabbit eyes, with and without PPV, and vitreous VEGF levels were measured as a function of time. Methods. The PPV group rabbits had a bilateral 25-gauge PPV, and in the no-PPV group, rabbits had intact vitreous. Intravitreal injections of hVEGF(165) were performed, and the animals were euthanatized at time points up to 7 days. The vitreous was isolated and an enzyme-linked immunosorbent assay was used to measure the VEGF levels. Pharmacokinetic parameters were determined in a noncompartmental analysis approach. Results. Mean vitreous VEGF levels decreased more rapidly in eyes subjected to PPV than in no-PPV eyes. The vitreous VEGF half-life (t([)(1/2)(])) in PPV eyes was 10 times shorter than that in normal eyes. In addition, mean clearance and mean area under the curve (AUC) increased and decreased, respectively, in eyes that underwent PPV. Conclusions. VEGF clearance is increased after PPV. Reducing VEGF concentrations in the vitreous post-PPV may partially explain the improvement in macular thickness in some patients with ME. Unexpectedly, the half-life of VEGF in the vitreous, even in no-PPV eyes, was <3 hours, whereas compounds of similar molecular weight typically have longer vitreous half-lives. The back of the eye may be uniquely adapted with rapid-clearance mechanisms to regulate vitreous VEGF levels. Further study is suggested.
Comparative analysis of targeted next-generation sequencing panels for the detection of gene mutations in chronic lymphocytic leukemia: an ERIC multi-center study (2020)

Next-generation sequencing (NGS) has transitioned from research to clinical routine, yet the comparability of different technologies for mutation profiling remains an open question. We performed a European multicenter (n=6) evaluation of three amplicon-based NGS assays targeting 11 genes recurrently mutated in chronic lymphocytic leukemia. Each assay was assessed by two centers using 48 pre-characterized chronic lymphocytic leukemia samples; libraries were sequenced on the Illumina MiSeq instrument and bioinformatics analyses were centralized. Across all centers the median percentage of target reads ≥100x ranged from 94.2- 99.8%. In order to rule out assay-specific technical variability, we first assessed variant calling at the individual assay level i.e., pairwise analysis of variants detected amongst partner centers. After filtering for variants present in the paired normal sample and removal of PCR/sequencing artefacts, the panels achieved 96.2% (Multiplicom), 97.7% (TruSeq) and 90% (HaloPlex) concordance at a variant allele frequency (VAF) >0.5%. Reproducibility was assessed by looking at the inter-laboratory variation in detecting mutations and 107 of 115 (93% concordance) mutations were detected by all six centers, while the remaining eight variants (7%) were undetected by a single center. Notably, 6 of 8 of these variants concerned minor subclonal mutations (VAF <5%). We sought to investigate low-frequency mutations further by using a high-sensitivity assay containing unique molecular identifiers, which confirmed the presence of several minor subclonal mutations. Thus, while amplicon-based approaches can be adopted for somatic mutation detection with VAF >5%, after rigorous validation, the use of unique molecular identifiers may be necessary to reach a higher sensitivity and ensure consistent and accurate detection of low-frequency variants.
Nanofiltration treatment for boron removal from ceramic industry wastewater (2018)

International audience
Metamodeling functional and interactive parts of systems for composition considerations (2011)

Software Composition techniques improve the reusability of systems built by composing smaller software artifacts. Composition is a challenge for Human Computer Interaction and Software Engineering communities. These communities have proposed metamode
Dual-emitting zein-protected gold nanoclusters for ratiometric fluorescence detection of Hg2+/Ag+ ions in both aqueous solution and self-assembled protein film (2019)

An abundant plant-sourced protein, zein, is used to prepare fluorescent Au nanoclusters as a promising alternative to animal/microorganism proteins.
Thermodynamic Modelling for Prediction of Asphaltene Deposition in Live Oils (1995)

ABSTRACT Original phase equilibria data on asphaltenic mixtures show that the principal modellistic approaches described in the literature do not accurately reproduce asphaltene phase behaviour. A new, more reliable modellistic approach is proposed in this paper, and a procedure for predicting the stability of asphaltene solutions is outlined. Application of the new model to two oils gives satisfactory results when compared with field observations.