gebhart/patrepeval huggingface dataset:
Enhancement of Neonatal Innate Defense: Effects of Adding an N-Terminal Recombinant Fragment of Bactericidal/Permeability-Increasing Protein on Growth and Tumor Necrosis Factor-Inducing Activity of Gram-Negative Bacteria Tested in Neonatal Cord Blood Ex Vivo (2000)

Innate defense against microbial infection requires the action of neutrophils, which have cytoplasmic granules replete with antibiotic proteins and peptides. Bactericidal/permeability-increasing protein (BPI) is found in the primary granules of adult neutrophils, has a high affinity for lipopolysaccharides (or "endotoxins"), and exerts selective cytotoxic, antiendotoxic, and opsonic activity against gram-negative bacteria. We have previously reported that neutrophils derived from newborn cord blood are deficient in BPI (O. Levy et al., Pediatrics 104:1327-1333, 1999). The relative deficiency in BPI of newborns raised the possibility that supplementing the levels of BPI in plasma might enhance newborn antibacterial defense. Here we determined the effects of addition of recombinant 21-kDa N-terminal BPI fragment (rBPI(21)) on the growth and tumor necrosis factor (TNF)-inducing activity of representative gram-negative clinical isolates. Bacteria were tested in citrated newborn cord blood or adult peripheral blood. Bacterial viability was assessed by plating assay, and TNF-alpha release was measured by enzyme-linked immunosorbent assay. Whereas adult blood limited the growth of all isolates except Klebsiella pneumoniae, cord blood also allowed logarithmic growth of Escherichia coli K1/r and Citrobacter koseri. Bacteria varied in their susceptibility to rBPI(21)'s bactericidal action: E. coli K1/r was relatively susceptible (50% inhibitory concentration [IC(50)], approximately 10 nM), C. koseri was intermediate (IC(50), approximately 1,000 nM), Klebsiella pneumoniae was resistant (IC(50), approximately 10,000 nM), and Enterobacter cloacae and Serratia marcescens were highly resistant (IC(50), >10,000 nM). All isolates were potent inducers of TNF-alpha activity in both adult and newborn cord blood. In contrast to its variable antibacterial activity, rBPI(21) consistently inhibited the TNF-inducing activity of all strains tested (IC(50), 1 to 1,000 nM). The antibacterial effects of rBPI(21) were additive with those of a combination of conventional antibiotics typically used to treat bacteremic newborns (ampicillin and gentamicin). Whereas ampicillin and gentamicin demonstrated little inhibition of bacterially induced TNF release, addition of rBPI(21) either alone or together with ampicillin and gentamicin profoundly inhibited release of this cytokine. Thus, supplementing newborn cord blood with rBPI(21) potently inhibited the TNF-inducing activity of a variety of gram-negative bacterial clinical pathogens and, in some cases, enhanced bactericidal activity. These results suggest that administration of rBPI(21) may be of clinical benefit to neonates suffering from gram-negative bacterial infection and/or endotoxemia.
Application of a gut-immune co-culture system for the study of N -glycan-dependent host–pathogen interactions of Campylobacter jejuni (2019)

An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity. The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system. The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity. Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs. The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays. Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others. Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model. This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.
Mixture Synthesis and “Charge Tagging” Based Demixing: An Efficient Strategy for the Preparation of Heparan Sulfate Libraries (2008)

ADVERTISEMENT RETURN TO ISSUEPREVReportNEXTMixture Synthesis and "Charge Tagging" Based Demixing: An Efficient Strategy for the Preparation of Heparan Sulfate LibrariesAnna Dilhas, Ricardo Lucas, Latino Loureiro-Morais, Yaël Hersant, and David Bonnaffé*View Author Information Univ Paris-Sud, Laboratoire de Chimie Organique Multifonctionnelle, Equipe de Glycochimie Moléculaire et Macromoléculaire, Institut de Chimie Moléculaire et des Matériaux d'Orsay, UMR CNRS-UPS 8182, Bat. 420, UPS, 91405 Orsay Cedex, France* To whom correspondence should be addressed. Phone: 33 (0)1 69 15 72 33 . Fax: 33 (0)1 69 15 47 15 . E-mail: [email protected]Cite this: J. Comb. Chem. 2008, 10, 2, 166–169Publication Date (Web):February 16, 2008Publication History Received4 January 2008Published online16 February 2008Published inissue 1 March 2008https://pubs.acs.org/doi/10.1021/cc8000019https://doi.org/10.1021/cc8000019brief-reportACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views572Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Chromatography,High-performance liquid chromatography,Mixtures,Post-translational modification,Purification Get e-Alerts
A Design Science Approach For Developing And Evaluating A Competence Acquisition Mobile App (2015)

The continuous development and acquisition of competences by a firm’s employee are fundamental instruments for achieving sustainable competitive advantage. In this scenario, digital technologies play a relevant role in providing a ubiquitous platform to foster and facilitate this learning process. This paper describes the design, development and evaluation process of a competence acquisition mobile app. The evaluation of the app was carried out through an experimentation involving middle managers as well as project managers form an IT consulting firm. The results indicate that the use of the mobile app effectively improved users’ learning process outcomes as well as the ability to deploy the new competence – demonstrating that an ubiquitous learning environment can be paramount for the development of competence acquisition tools.
Comparison of Anesthetic Depth Indexes Based on Thalamocortical Local Field Potentials in Rats (2010)

The permutation entropy corrected with the classic burst suppression ratio is a promising alternative to other indexes of anesthetic depth. Furthermore, high coherence level of activity exists between the somatosensory cortical and thalamic regions, even at deep isoflurane stages.
Synthetic dimyristoylphosphatidylcholine liposomes assimilating into high-density lipoprotein promote regression of atherosclerotic lesions in cholesterol-fed rabbits (2010)

We have reported recently that enrichment of high-density lipoprotein (HDL) with phosphatidylcholine (PC) liposomes is effective in solubilizing cholesterol from isolated human atherosclerotic plaques. In the present study, we investigated the in vivo effect of enrichment of HDL with PC on regression of diet-induced atherosclerosis in rabbits. As part of the study, a preliminary in vitro study on blood collected from the cholesterol-fed rabbits was performed to assess the capacity of the HDL density (d > 1.063 g/mL) plasma fraction from cholesterol-fed rabbits to assimilate multilamellar liposomes of synthetic dimyristoylphosphatidylcholine (DMPC). This was compared with the capacities of egg- and soy-PC liposomes to be assimilated into the HDL density plasma fraction. The capacity of the HDL density fraction to absorb PC from DMPC liposomes (11.5 mg/mL) was more than 10 times greater than egg or soy liposomes. Therefore, DMPC liposomes were chosen to infuse into cholesterol-fed rabbits. Cholesterol-fed rabbits infused weekly with DMPC liposomes (300 mg/kg body weight) for five weeks had significantly decreased aortic cholesterol contents (P < 0.05) compared with saline-infused cholesterol-fed controls. Atherosclerotic plaque volume, as measured by a type of new magnetic resonance imaging analysis, also decreased significantly (P < 0.05) after DMPC treatment. The present findings suggest that the enrichment of HDL with PC via intravenous infusion of synthetic DMPC liposomes could be a potential therapeutic approach for atherosclerotic plaque regression.
Analysis of Convective Mass Transfer by Potential Relaxation: II . Transient Natural Convection Copper Deposition (1990)

A transient potential relaxation technique was used to determine mass transfer boundary‐layer thicknesses during steady‐state and pre‐steady‐state natural convection copper deposition at a vertical flat plate cathode. Boundary‐layer thicknesses for constant currents below the limiting current and pulsed currents at 6 Hz were found by matching experimentally measured concentration overpotentials after current interruption to a theoretical surface concentration decay model. The initial growth of constant and pulsed current boundary‐layers with the time of current passage was found to be diffusion controlled. Steady‐state pulsed current boundary‐layer thicknesses were approximately 33% smaller than those obtained from constant current experiments.
Switching of 3300V Scaled IGBT by 5V Gate Drive (2019)

In this work, the switching of 3300V IGBTs by 5V gate drive voltage has been successfully demonstrated for the first time. IGBT was designed based on a scaling principle. Comparing with conventional 15V-driven non-scaled IGBTs, the tum-off tail current of the scaled devices significantly decreased. The improvement of Eoff vs Vcesat relationship by 35% was achieved.
Efficacy of Mycobacterium bovis BCG vaccination in mice undergoing prior pulmonary infection with atypical mycobacteria (1984)

The efficacy of Mycobacterium bovis BCG immunization in mice with established pulmonary infections caused by atypical mycobacteria was studied. In all four strains of Mycobacterium tested (M. kansasii, M. simiae, M. avium, and M. scrofulaceum), intravenous inoculation with 10(6) BCG had no discernible effect upon the course of atypical mycobacterial infection within the lungs; despite this, however, all BCG-vaccinated groups of mice were fully resistant to a subsequent acute aerogenic challenge with M. tuberculosis H37Rv, regardless of the presence of the pulmonary atypical mycobacterial infections. Furthermore, animals infected with M. kansasii, M. simiae, or M. avium but not vaccinated with BCG expressed considerable antituberculous resistance within the lungs, resulting in significant prolonged survival of these animals. The relevance of these findings to the expression of antituberculous resistance in human populations in areas in which atypical mycobacteria are endemic and the failure of these findings to support the hypothesis that prior contact with atypical mycobacteria might in some way jeopardize or interfere with the efficacy of subsequent BCG vaccination are discussed.
Tibiofibular synostosis procedure in the management of complex tibia fractures. (2005)

Complex tibia nonunions and defects caused by tumor, trauma, and congenital abnormality are difficult to treat. The tibiofibular synostosis procedure is a treatment option. Fifteen patients with complex diaphyseal tibia fractures (4 segmental, 11 comminuted; 10 open, 5 closed) were managed with this procedure. Mean time between injury and procedure was 20.3 weeks. Mean healing time was 16 weeks (acceptable); 14 of 15 cases showed healing by final follow-up (36 months). At final follow-up, each patient's knee range of motion (ROM) and ankle ROM were acceptable. Knee ROM varied from full extension to 150 degrees of flexion for 13 patients and from full extension to 120 degrees of flexion for 2 patients. Ankle dorsiflexion ranged from 0 degrees to 40 degrees for 10 patients, from 0 degrees to 30 degrees for 3 patients, and from 0 degrees to 20 degrees for 2 patients. There were no radiographic arthritic changes in any case. The tibiofibular synostosis procedure is effective in treating complex nonunions and defects.
Listeria monocytogenesσBRegulates Stress Response and Virulence Functions (2003)

While the stress-responsive alternative sigma factor sigma(B) has been identified in different species of Bacillus, Listeria, and Staphylococcus, the sigma(B) regulon has been extensively characterized only in B. subtilis. We combined biocomputing and microarray-based strategies to identify sigma(B)-dependent genes in the facultative intracellular pathogen Listeria monocytogenes. Hidden Markov model (HMM)-based searches identified 170 candidate sigma(B)-dependent promoter sequences in the strain EGD-e genome sequence. These data were used to develop a specialized, 208-gene microarray, which included 166 genes downstream of HMM-predicted sigma(B)-dependent promoters as well as selected virulence and stress response genes. RNA for the microarray experiments was isolated from both wild-type and Delta sigB null mutant L. monocytogenes cells grown to stationary phase or exposed to osmotic stress (0.5 M KCl). Microarray analyses identified a total of 55 genes with statistically significant sigma(B)-dependent expression under the conditions used in these experiments, with at least 1.5-fold-higher expression in the wild type over the sigB mutant under either stress condition (51 genes showed at least 2.0-fold-higher expression in the wild type). Of the 55 genes exhibiting sigma(B)-dependent expression, 54 were preceded by a sequence resembling the sigma(B) promoter consensus sequence. Rapid amplification of cDNA ends-PCR was used to confirm the sigma(B)-dependent nature of a subset of eight selected promoter regions. Notably, the sigma(B)-dependent L. monocytogenes genes identified through this HMM/microarray strategy included both stress response genes (e.g., gadB, ctc, and the glutathione reductase gene lmo1433) and virulence genes (e.g., inlA, inlB, and bsh). Our data demonstrate that, in addition to regulating expression of genes important for survival under environmental stress conditions, sigma(B) also contributes to regulation of virulence gene expression in L. monocytogenes. These findings strongly suggest that sigma(B) contributes to L. monocytogenes gene expression during infection.
Clinical presentation of adult coeliac disease. (2011)

The mode of presentation of coeliac disease has been changing to more atypical or silent disease. Few studies described the clinical presentation of adult coeliac disease in Ireland in recent years. We retrospectively collected the clinical data for all patients who had a diagnosis of coeliac disease made in our centre between January 07 and December 08. Forty seven adults, predominantly females (n = 30), had a confirmed diagnosis of coeliac disease made during the study period. In our patient cohort, the presenting symptom was diarrhoea in 19 (40%) patients, while 16 patients (34%) did not have any G.I. symptoms, 10 (21%) presented with anaemia. Females presented at a significantly younger age compared to males, with median ages at diagnosis of 44.5 and 57 years, respectively (p = 0.04). Females also presented more commonly with non G.I. symptoms (p = 0.07). The reasons behind this gender difference need further study.
Radio Frequency Tank Circuit for Probing Planar Lipid Bilayers (2013)

We present first results from a hybrid coplanar waveguide microfluidic tank circuit for monitoring lipid bilayer formation and fluctuations of integrated proteins. The coplanar waveguide is a radio frequency resonator operating at ~250 MHz. Changes within the integrated microfluidic chamber, such as vesicle bursting and subsequent nanopore formation alter the reflected signal, and can be detected with nanosecond resolution. We show experimental evidence of such alterations when the microfluidic channel is filled with giant unilamellar vesicles (GUVs). Subsequent settling and bursting of the GUVs form planar lipid bilayers, yielding a detectable change in the resonant frequency of the device. Results from finite element simulations of our device correlate well with our experimental evidence. These simulations also indicate that nanopore formation within the bilayer is easily detectable. The simulated structure allows for incorporation of microfluidics as well as simultaneous RF and DC recordings. The technique holds promise for high throughput drug screening applications and could also be used as an in-plane probe for various other applications. It opens up possibilities of exploring ion channels and other nano scale pores in a whole new frequency band allowing for operating at bandwidths well above the traditional DC methods.
Structural variations of N‐acetylneuraminic acid, 15. Synthesis of 9‐deoxy‐, 7,9‐dideoxy‐, and 4,7,9‐trideoxy‐N‐acetylneuraminic acid and their behaviour towards CMP‐sialate synthase (1990)

Abstract The methyl ester of N ‐acetylneuraminic acid β‐methyl ketoside (Neu5Ac1Me‐2β‐Me) ( 1 ) is used as common starting material for the synthesis of the title compounds. Combined derivatization reactions with reagents thiophosgene, p ‐cresol, benzoyl chloride, and acetic anhydride/pyridine lead to the thiocarbonate derivatives 2 , 6 – 8 , and 12 . Further transformation with iodomethane yields the 9‐iodo compounds 3, 9 , and 13 , which are reduced by tributyltin hydride to the deoxy derivatives 4 , 10 , and 14 . Hydrolysis of 14 , 10 , and 4 affords the desired 9‐deoxy, 7,9‐dideoxy‐, and 4,7,9‐trideoxy‐ N ‐acetylneuraminic acids 15 (9‐d‐Neu5Ac), 11 (7,9‐d 2 ‐Neu5Ac), and 5 (4,7,9‐d 3 ‐Neu5Ac), respectively. — The sialic acid analogues 11 and 5 were activated by CMP‐sialate synthase [E,C.2.7.7.43] to an extent of 30–40 and 50–60%, respectively, relative to N ‐acetylneuraminic acid.
Type 2 MI induced by a single high dose of isoproterenol in C57BL/6J mice triggers a persistent adaptive immune response against the heart (2020)

Heart failure is the common final pathway of several cardiovascular conditions and a major cause of morbidity and mortality worldwide. Aberrant activation of the adaptive immune system in response to myocardial necrosis has recently been implicated in the development of heart failure. The ß-adrenergic agonist isoproterenol hydrochloride is used for its cardiac effects in a variety of different dosing regimens with high doses causing acute cardiomyocyte necrosis. To assess whether isoproterenol-induced cardiomyocyte necrosis triggers an adaptive immune response against the heart, we treated C57BL/6J mice with a single intraperitoneal injection of isoproterenol. We confirmed tissue damage reminiscent of human type 2 myocardial infarction. This is followed by an adaptive immune response targeting the heart as demonstrated by the activation of T cells, the presence of anti-heart auto-antibodies in the serum as late as 12 weeks after initial challenge and IgG deposition in the myocardium. All of these are hallmark signs of an established autoimmune response. Adoptive transfer of splenocytes from isoproterenol-treated mice induces left ventricular dilation and impairs cardiac function in healthy recipients. In summary, a single administration of a high dose of isoproterenol is a suitable high-throughput model for future studies of the pathological mechanisms of anti-heart autoimmunity and to test potential immunomodulatory therapeutic approaches.
Endoplasmic reticulum stress regulates immunosuppressive function of myeloid derived suppressor cells in leprosy (2019)

Abstract Myeloid derived suppressor cells (MDSC) are a heterogeneous population of immature myeloid cells defined by their phenotype along with the ability to suppress adaptive T cell responses. Although MDSC are expanded in response to microbial infection, the factors that regulate the suppressive function of MDSC in this context are not known. Here, we studied MDSC in patients with leprosy, a chronic infectious disease caused by Mycobacterium leprae, in which the clinical manifestations including bacterial load present on a spectrum that correlates with the type of immune response. We found cells with a granulocytic MDSC phenotype are increased in patients across the spectrum of leprosy. However, only MDSC from leprosy patients with disseminated multibacillary infection, not patients with localized paucibacillary disease or healthy controls, suppress M. leprae- specific T cell proliferation and IFN-γ production. An increase in an ER stress gene signature was seen in MDSC from multibacillary patients vs paucibacillary patients. Additionally, the immunosuppressive function of MDSC from multibacillary patients was reversed by either treatment with a free radical scavenger or blockade of interleukin (IL)-10. Conversely, induction of ER stress in MDSC-like cells from paucibacillary patients was sufficient to induce suppressor activity. These studies of human leprosy link multibacillary infection, ER stress and MDSC suppressive function.
Identification des propriétés radiatives et du champ de température de matériaux fibreux de faible densité. Application aux barrières thermiques hautes températures (2014)

National audience
Dopant-induced bandgap shift in Al-doped ZnO thin films prepared by spray pyrolysis (2012)

A series of 1 at. % Al-doped ZnO (AZO) films were deposited onto glass substrates by a spray pyrolysis technique. We find that the observed blue shift in the optical bandgap of 1% AZO films is dominated by the Burstein Moss effect. The Fermi level for an 807 nm thick AZO film rose by some 0.16 eV with respect to the edge of the conduction band. By controlling the film thickness, all AZO films exhibit the same lattice strain values. The influence of strain-induced bandgap shift was excluded by selecting films with nearly the same level of bandgap volume-deformation potentials, and the differences in out-plain strain and in-plain stress remained effectively constant.