gebhart/patrepeval huggingface dataset:
Identification of tumor antigens in renal cell carcinoma by serological proteome analysis (2001)

We have investigated the suitability of proteomics for identification of tumor-associated antigens. First, we compared the proteomes of nontumorous kidney and renal cell carcinoma (RCC) by two-dimensional gel electrophoresis (2-DE) and silver staining. Protein patterns were markedly different (approximately 800 spots in RCCs versus approximately 1400 spots in kidney). 2-DE immunoblotting revealed five RCC-specific spots, reproducibly reactive with RCC-patient but not healthy donor control sera. Two of these antigens were isolated by preparative 2-DE, and identified by Edman sequencing of tryptic peptides. The first antigen, smooth muscle protein 22-alpha (SM22-alpha), is an actin-binding protein of unknown function predominantly expressed in smooth muscle cells. In situ hybridization revealed that SM22-alpha is not expressed in the malignant cells but in mesenchymal cells of the tumor stroma. The second antigen represents carbonic anhydrase I (CAI), an isoform usually not expressed in kidney. Interestingly, a different isoform (CAXII) has previously been identified by serological expression cloning as an antigen overexpressed in some RCCs. In additional assays, antibodies to recombinant CAI or SM22-alpha were detected in sera from 3/11 or 5/11 RCC patients, respectively, whereas sera from 13 healthy individuals did not react. In conclusion, serological proteome analysis may be a new tool for the identification of tumor-associated antigens.
Semi-autonomous Map-making and Navigation (1992)

Map construction and task-oriented navigation constitute two important capabilities for mobile robots. Despite their value, progress to date in the development of stand-alone robots endowed with these capabilities has been limited. We describe an architecture for mobile robots based on the concept of semi-autonomy that employs communication with a human advisor in order to simplify the tasks of map construction and navigation. Communication can be used both by the human to convey information to the robot that is beyond its own perceptual capabilities and by the robot to extract information from the advisor that may be required in the execution of an assigned task. The semiautonomous philosophy has two main benefits, namely a de-emphasis on the notoriously difficult subtask of perception and an overall increase in flexibility.
Supplementary Figure 1 from Inhibition of DNA Double-Strand Break Repair by the Dual PI3K/mTOR Inhibitor NVP-BEZ235 as a Strategy for Radiosensitization of Glioblastoma (2023)

&lt;p&gt;PDF file - 246K, NVP-BEZ235 inhibits the DDR in U87-vIII cells.&lt;/p&gt;
Adaptive Output Feedback Based on Closed-Loop Reference Models for Hypersonic Vehicles (2015)

This paper presents a new method of synthesizing an output feedback adaptive controller for a class of uncertain, nonsquare multi-input/multioutput systems that often occur in hypersonic vehicle models. The main challenge that needs to be addressed is the determination of a corresponding square and strictly positive real transfer function. This paper proposes a new procedure to synthesize two gain matrices that allow the realization of such a transfer function, thereby allowing a globally stable adaptive output feedback law to be generated. The unique features of this output feedback adaptive controller are a baseline controller that uses a Luenberger observer, a closed-loop reference model, manipulations of a bilinear matrix inequality, and the Kalman–Yakubovich lemma. Using these features, a simple design procedure is proposed for the adaptive controller, and the corresponding stability property is established. The proposed adaptive controller is compared to the classical multi-input/multioutput adaptive controller. A numerical example based on a scramjet-powered blended-wing/body generic hypersonic vehicle model is presented. The six-degree-of-freedom nonlinear vehicle model is linearized, giving the design model for which the controller is synthesized. The adaptive output feedback controller is then applied to an evaluation model, which is nonlinear, coupled, and includes actuator dynamics; and it is shown to result in stable tracking in the presence of uncertainties that destabilize the baseline linear output feedback controller.
Fibrolase. A fibrinolytic protein from snake venom. (1993)

Fibrolase is an active fibrinolytic agent and possesses potential for use in thrombolytic therapy. Its mode of action had been characterized, both in vitro and in vivo. Possessing three disulfide bonds, native fibrolase is nonglycosylated and binds an intrinsic zinc atom. The zinc is essential for retention of activity and structural integrity. In solution, fibrolase is sensitive to changes in pH and temperature (Pretzer et al., 1991). At neutral to basic pH (pH 5-9), the solubility and stability of fibrolase is nearly constant. Little structural variation can be detected by CD spectroscopy. However, decrease in pH below 5 leads to a pronounced reduction in both the solubility and activity of fibrolase. At pH 3 and below, the solubility of fibrolase returns but the activity does not. This solubility profile is unusual in that the minimal solubility is well removed from the pI (which is 6.7). It is proposed that the behavior of fibrolase with variation in pH can be understood in terms of capacity to bind zinc. At pH 5 to 9, the protein binds zinc and the structure and activity are preserved. Near pH 5, the histidine residues which serve as ligands for the zinc become protonated and zinc binding is lost. Loss of zinc leads to local unfolding of a helical segment of fibrolase, exposing hydrophobic groups which allow the protein to rapidly aggregate. At lower pH values (1-3), the protein again adopts a more globular structure, similar to molten globule states, and the solubility increases. However, without the zinc, fibrolase remains inactive. Changes in pH also affect thermal stability. The Tm for fibrolase moves from 50 degrees C at pH 8 to 43 degrees C at pH 5. Increases in temperature also lead to removal of the zinc ion, again producing a partially denatured protein with a marked tendency to aggregate. In both cases (decrease in pH and increase in temperature), analysis of the CD spectra indicates that the protein has primarily lost alpha-helical secondary structure. A major change in structure can also be observed using NMR spectroscopy. At temperatures below 35 degrees C, the globular structure of fibrolase remains intact, although some increase in chain mobility can be noted with increased temperature. Upon melting, numerous signals collapse as the protein unfolds. Transition temperatures (Tm) as measured by CD and NMR are in good agreement. Similar structural changes can be induced by adding zinc chelators such as EDTA and DTT. This leads to complete loss of activity at EDTA concentrations above 1.0 mM.(ABSTRACT TRUNCATED AT 400 WORDS)
Summary Report of the First International Symposium on Strain Gauge Balances and Workshop on AoA/Model Deformation Measurement Techniques (1997)

The first International Symposium on Strain Gauge Balances was sponsored under the auspices of the NASA Langley Research Center (LaRC), Hampton, Virginia during October 22-25, 1996. Held at the LaRC Reid Conference Center, the Symposium provided an open international forum for presentation, discussion, and exchange of technical information among wind tunnel test technique specialists and strain gauge balance designers. The Symposium also served to initiate organized professional activities among the participating and relevant international technical communities. The program included a panel discussion, technical paper sessions, tours of local facilities, and vendor exhibits. Over 130 delegates were in attendance from 15 countries. A steering committee was formed to plan a second international balance symposium tentatively scheduled to be hosted in the United Kingdom in 1998 or 1999. The Balance Symposium was followed by the half-day Workshop on Angle of Attack and Model Deformation on the afternoon of October 25. The thrust of the workshop was to assess the state of the art in angle of attack (AoA) and model deformation measurement techniques and to discuss future developments
Risk of malignancy associated with diagnostic categories of the proposed World Health Organization International System for Reporting Pancreatobiliary Cytopathology (2021)

In 2014, the Papanicolaou Society of Cytopathology (PSC) published terminology guidelines for reporting pancreatobiliary cytology. Recently, a group of international experts convened to update the PSC system for reporting pancreatobiliary cytology. The proposed new system will be named the World Health Organization (WHO) International System. This study retrospectively assesses the risk of malignancy in a previously studied cohort of patients using the proposed WHO International System for reporting pancreatobiliary cytology.
Ruminal lactic acidosis in sheep and goats (1992)

The clinical findings in 37 sheep and goats with acute ruminal lactic acidosis included a disturbed general condition characterised by anorexia, apathy, teeth grinding and muscle twitching, ruminal stasis, and the excretion of soupy or watery faeces. The ruminal fluid of affected animals was milky, had a sour odour and a low pH. There was a predominance of Gram-positive bacteria in smears of ruminal fluid. In comparison with 10 control animals, the rumen fluid of 23 sheep with ruminal lactic acidosis had higher lactic acid and lower volatile fatty acid concentrations. In addition, the affected animals often had haemoconcentration and metabolic acidosis. Treatment included single or repeated transfer of ruminal fluid from healthy cows and, depending on the severity, the administration of antacids, yeast and chlortetracycline, and the intravenous infusion of isotonic sodium chloride and 5 per cent sodium bicarbonate solutions. Of the 37 treated sheep and goats, four died within 24 hours, and three others were euthanased after one, two and three days because their condition rapidly deteriorated. Thirty animals were discharged one to nine days after treatment. Twenty-nine of them (78.4 per cent) recovered completely but one was euthanased later.
Node.js: Using JavaScript to Build High-Performance Network Programs (2010)

One of the more interesting developments recently gaining popularity in the server-side JavaScript space is Node.js. It's a framework for developing high-performance, concurrent programs that don't rely on the mainstream multithreading approach but use asynchronous I/O with an event-driven programming model.
The Therapeutic Implications of Intratumoral Regulatory T Cells (2005)

In this issue of Clinical Cancer Research , Wolf and colleagues present compelling evidence that underscores a key role for regulatory T cell–induced immune suppression in tumor pathogenesis ([1][1]). Although most cancer patients mount innate and adaptive antitumor reactions, the evolution of
Mechanism of Ionic Recognition by Polymer-Supported Reagents: Immobilized Tetramethylmalonamide and the Complexation of Lanthanide Ions (2010)

The mechanism of ionic recognition by polymer-supported reagents is probed with cross-linked polystyrene modified with tetramethylmalonamide (TMMA) ligands. The substrates are lanthanide ions in 0.001-8 M HCl and HNO(3) solutions. The results fall into three regions of acid concentration: low, mid, and high. In HCl, distribution coefficients are low in 0.001 to 2 M, increase in 4 and 6 M, and then decrease in 8 M HCl. In the low-acid region, the metal ion remains with its waters of hydration and does not coordinate to the carbonyl oxygens. As the acid concentration exceeds 2 M, protonation of the amide occurs to form an iminium moiety, electrostatically attracting the anionic lanthanide complex through ion-exchange and releasing waters of hydration. At high acid concentration, the apparent affinity decreases due to competition by the large excess of chloride ions for the ion-exchange sites. The affinity sequence in 6 M HCl is Tb > Dy > Eu > Gd > Ho > Sm > Er > Tm > Yb > Lu > Nd > Ce > La. The TMMA-Ln interaction is due to recognition since there is a point of maximum affinity across the series rather than a monotonic trend. The trends are comparable in HNO(3). A comparison of the distribution coefficients at the maxima (6 M HCl and 4 M HNO(3)) shows nitrate to have greater values than chloride due to a hydration effect, as also indicated by results from H(2)SO(4).
Value of thyroid hormone determination. (1989)

Free thyroid hormone assays have broadened the repertoire in thyroid diagnosis. At elevated binding protein levels they provide more reliable information on the metabolic state than do total hormone determinations; yet under estrogen contraception free T4 may yield test specifically falsely too high data. As a rule, there is accordance between free hormone concentrations and the indices, being equivalent in establishing the free thyroid hormone fraction. In this light the Thyroid Section of the German Endocrinologic Society recommended in 1986 to consider determinations of free or total hormone concentrations with calculation of the indices as being of equal value. In borderline situations the TRH test, the hypersensitivity TSH assay as well as checkups establishing the course of development of the disorder are substantiated.
Demand-based apportionment on electricity payment of hvac systems (2014)

Heating, Ventilation, and Air Conditioning (HVAC) systems account for a significant portion of power consumption in commercial buildings all over the world [1, 4, 5, 6]. Since HVAC systems are designed for centralized cooling supplies, power consumption in each room is inestimable. Therefore, administrators are motivated to apportion the electricity payment for actual users. The even apportionment is an intuitive way. Previous work implies that the even apportionment does not encourage people to reduce power consumption [2]. Some researchers investigate a variety of policies to apportion the power usage and then conclude that understanding the personal energy use is an incentive to reduce power consumption [3]. However, the pay-as-you-go based apportionment is unrealistic on HVAC systems because of the difficulty on measuring the individual power consumption. In addition, climate differences cause the unfairness on the pay-as-you-go based apportionment. The reason is that the two rooms need various amount of cooling supplies to achieve the same indoor temperature. The difference on cooling supplies in the rooms is led by the environment. For example, a room in the top level always needs more supplies against the sun exposure. Under the pay-as-you-go based apportionment, no one wants to use the rooms in the top level. Therefore, we propose the demand-based apportionment to
High-Performance Quantum Dot Lasers and Integrated Optoelectronics on Si This review of lasers, grown directly on silicon to perform electrical to optical signal conversion, finds that these temperature-stable devices can be integrated with other optical devices. (2009)

This paper provides a review of the recent dev- elopments of self-organized In(Ga)As/Ga(Al)As quantum dot lasers grown directly on Si, as well as their on-chip integration with Si waveguides and quantum-well electroabsorption mod- ulators. A novel dislocation reduction technique, with the incorporation of self-organized In(Ga,Al)As quantum dots as highly effective three-dimensional dislocation filters, has been developed to overcome issues associated with the material incompatibility between III-V materials and Si. With the use of this technique, quantum dot lasers grown directly on Si exhibit relatively low threshold current ðJth ¼ 900 A/cm 2 Þ and very high temperature stability ðT0 ¼ 278 KÞ. Integrated quantum dot lasers and quantum-well electroabsorption modulators on Si have been achieved, with a coupling coefficient of more than 20% and a modulation depth of � 100% at a reverse bias of 5 V. The monolithic integration of quantum dot lasers with both amorphous and crystalline Si waveguides, fabricated using plasma-enhanced chemical-vapor deposition and membrane transfer, respectively, has also been demonstrated.
RECODIS: Resilient Communication Services Protecting End-user Applications from Disaster-based Failures (2016)

Disaster-based failures can seriously disrupt any communication network, making its services unavailable. Such disruptions may be caused by natural disasters, technology-related failures, or malicious attacks, and they are observably increasing in number, intensity and scale. When network services that are a part of critical infrastructure become unavailable, commercial and/or societal problems are inevitable. The issue of limiting the impact of disaster-based failures needs to be urgently addressed due to the lack of suitable mechanisms deployed in the current networks. The COST CA15127 (RECODIS) Action will fill this gap by developing appropriate solutions to provide cost-efficient resilient communications in the presence of disaster-based disruptions considering both existing and emerging communication network architectures. It will be driven by researchers from academia and industry in strong cooperation with governmental bodies. In this paper, we highlight the objectives of RECODIS, its structure, as well as planned outcomes.
Joining the design and mask flows for better and cheaper masks (2004)

Today's design-manufacturing interfaces have only minimal information exchange. Lack of information on either side leads to under-performance due to too much guardbanding, and increased mask cost and increased turnaround time due to over-correction. In this work we present techniques that simultaneously utilize design and manufacturing information to improve mask quality and reduce mask cost.
Cerebral blood flow, oxidative metabolism, and cerebrovascular carbon dioxide reactivity in patients with acute bacterial meningitis (2000)

European Society of Anaesthesiologists; 8th Annual Meeting with the Austrian International Congress; Vienna, Austria, 1-4 April 2000
Resistance of Muscle to Tumor Metastases: A Role for A3 Adenosine Receptor Agonists (2001)

Tumor metastases are extremely rare in striated muscles. Lately, we have found that muscle cell conditioned medium (MCM) inhibits the proliferation of various tumor cells while maintaining the growth of normal murine bone marrow cells. This dual activity was confirmed in vivo when the MCM was administered orally, i.e., it inhibited the development of tumor growth in mice and prevented the myelotoxic effects of chemotherapy. Adenosine was found to be one of the active components of MCM, inhibiting tumor cell growth while maintaining bone marrow cell proliferation in vitro. Adenosine is known to act as an important regulatory molecule through its binding to specific G-protein-associated A1, A(2a), A(2b) and A3 cell surface receptors. In distinction from MCM, adenosine did not suppress tumor development in mice and was not active as a chemoprotective agent when administered orally or intravenously. Thus, the in vivo activity of MCM could not be attributed to adenosine. In this study, MCM from which adenosine was enzymatically removed still retained its dual activity that was also found to be mediated through the A3 adenosine receptor (A3AR). This result led to the conclusion that natural agonists to A3AR were responsible for the activity of MCM. We further tested synthetic agonist to the A3AR and demonstrated that it possessed the same in vitro and in vivo activity profile as MCM. Taken together, muscle cells, in addition to adenosine, secrete natural agonists to A3AR. These agonists are stable nondegradable molecules and may contribute to the systemic anticancer and chemoprotective activity exerted by MCM. This group of molecules may account for the rarity of tumor metastases in muscle.
Different Kinetic Pathways of the Binding of Two Biphenyl Analogues of Colchicine to Tubulin (1996)

The kinetics of the interaction of tubulin with two biphenyl analogues of colchicine were measured by fluorescence stopped flow. The ligands were 2,3,4-trimethoxy-4'-carbomethoxy-1,1'-biphenyl (TCB) and 2,3,4-trimethoxy-4'-acetyl-1,1'-biphenyl (TKB). The binding of both analogues is accompanied by a fluorescence increase with monophasic kinetics, which indicates that these drugs, unlike colchicine, do not discriminate between the isoforms of tubulin. The observed pseudo-first-order rate constant increases in a nonlinear way with the drug concentration, indicating that the binding of the biphenyl analogues to tubulin occurs, like colchicine, in two steps: a fast reversible equilibrium followed by an isomerization of the initial complex. Kinetic analysis shows that TCB and TKB exhibit differences in their K1 values. At 25 degrees C, these are 114,000 +/- 15,000 M(-1) for TCB and 8,300 +/- 900 M(-1) for TKB. Both molecules show a much higher affinity than colchicine for the initial binding site. Also at 25 degrees C, the k2 value is 0.66 +/- 0.04 s(-1) for TCB and 3.0 +/- 0.2 s(-1) for TKB. From the temperature dependence, a reaction enthalpy change for the initial binding (deltaH(zero)1) of 44 +/- 9 kJ x mol(-1) (TCB) and -40 +/- 14 kJ x mol(-1) (TKB) and an activation energy for the second forward step of 64 +/- 2 kJ x mol(-1) (TCB) and 101 +/- 10 kJ x mol(-1) (TKB) were calculated. The dissociation kinetics were studied by displacement experiments, in which podophyllotoxin was used as a displacing ligand. The rate constant for the second step in the off direction (k(-2)) is 0.25 +/- 0.05 s(-1) for TCB and 0.093 +/- 0.009 s(-1) for TKB at 25 degrees C. The activation energies for the backward isomerization of the complexes were found to be 86 +/- 20 kJ x mol(-1) (TCB) and 79 +/- 5 kJ x mol(-1) (TKB). Combination of these results with the kinetic parameters for association gives a full characterization of the enthalpy pathway for the binding of TCB and TKB. The pathway of TCB binding is shown to differ considerably from that of TKB binding. Since their structural difference is located in ring C', this result points to their use of the ring C' in the first binding step. The competitiveness of the binding of TCB and TKB with those of podophyllotoxin, MTC, and MDL 27048 indicates that the two biphenyls interact as well with the trimethoxyphenyl-specific subsite.
Excitation-contraction coupling in postischemic myocardium. Does failure of activator Ca2+ transients underlie stunning? (1990)

To elucidate the mechanism of contractile dysfunction in postischemic ("stunned") myocardium, time-resolved measurements of intracellular free Ca2+ concentration ([Ca2+]i) were made using gated 19F nuclear magnetic resonance in seven perfused ferret hearts loaded with the fluorinated Ca2+ indicator 5F-BAPTA. Left ventricular developed pressure decreased to 65 +/- 3% (mean +/- SEM) of control after 15 minutes of global ischemia at 37 degrees C. In stunned myocardium, diastolic [Ca2+]i (0.24 +/- 0.03 microM) was not changed from control (0.18 +/- 0.03 microM, p greater than 0.10), but peak [Ca2+]i (1.03 +/- 0.13 microM) was paradoxically higher than that in control (0.61 +/- 0.06 microM, p less than 0.02). The slope of the relation between developed pressure and Ca2+ transient amplitude in stunned myocardium was significantly lower than that in control (p less than 0.05), even after normalization by maximal Ca2(+)-activated pressure. These results indicate that contractile failure in stunned myocardium is due to a decrease in the myofilament sensitivity to Ca2+ as well as to the previously identified decrease in maximal Ca2(+)-activated force; failure of activator Ca2+ delivery cannot be implicated. The increase in the amplitude of Ca2+ transients would require that more ATP be spent in Ca2+ sequestration; thus, decreased efficiency of energy utilization in stunned myocardium would result.