gebhart/patrepeval huggingface dataset:
Abstract P367: ‘Youthful’ Phenotype Of C-kit+ Cardiac Fibroblasts (2021)

Introduction: Fibroblasts are critical contributors to myocardial development, tissue homeostasis and remodeling. Cardiac fibroblast population heterogeneity and plasticity present a challenge for categorization of biological and functional properties. Distinct molecular markers and associated signaling pathways provide valuable insight for cardiac fibroblast biology and interventional strategies to influence injury response and remodeling. Receptor tyrosine kinase c-Kit mediates cell survival, proliferation, migration, and is activated by pathological injury. However, the biological significance of c-Kit within cardiac fibroblast population has not been addressed. Approach: An inducible c-Kit reporter mouse detects promoter activation with Green Fluorescent Protein (GFP) expression in cardiac interstitial cells (CICs). Coincidence of GFP and c-Kit with the DDR2 fibroblast marker was confirmed at protein level using flow cytometry and immunohistochemistry. Subsequently, cardiac fibroblasts expressing DDR2 with or without c-Kit were isolated and characterized in vitro . Results: A subset of DDR2 + cardiac fibroblasts also express c-Kit with coincidence in ~8% of total CICs. Pathological injury induces coincidence as well as expression of c-Kit and DDR2. Cultured cardiac DDR2+ fibroblasts that are c-Kit+ exhibit youthful morphological and functional phenotypes compared to c-Kit- cells including 1) significantly smaller size, 2) higher cellular motility, 3) enhanced proliferation, 4) less multinucleation, 5) decreased senescence-associated β-galactosidase staining, and 6) down-regulation of p53 senescence marker. Mechanistically, c-Kit expression correlates with signaling implicated in proliferation and cell migration including phospho-ERK and pro-Caspase 3. Conclusion: The phenotype of c-kit+ on DDR2+ cardiac fibroblasts correlates with multiple characteristics of ‘youthful’ cells. To our knowledge, this represents the first evaluation of c-Kit biology within DDR2+ cardiac fibroblast population and provides a fundamental basis for future studies to influence myocardial biology, cardiac remodeling, and response to pathological injury.
Tunneling gate oxide MOSFET technology (1997)

Characteristics of direct tunneling gate oxide MOSFETs are described. The effect of the gate leakage current on the MOSFET characteristics be­ comes small as gate length reduces. Extremely high DC and AC perform­ ances were realized using such an ultra-thin oxide down to 1.5 nm. Higher reliability in hot-carrier and TDDB has been also observed.
Advanced Zirconium Alloy for PWR Application (2010)

Abstract Westinghouse is evaluating several advanced zirconium-based alloys, designated collectively as AXIOM™, to achieve improved performance for more demanding fuel management schemes. There are five candidate AXIOM alloys currently being evaluated by Westinghouse. The in–pressurized water reactor (PWR) performance of one candidate alloy, X5A, is reviewed in this paper. The irradiation performance of X5A (previously identified as Alloy A) cladding that was fabricated using high-temperature processing (HTP) was published in 2002. Since then, the fabrication process of X5A was optimized by the use of a low-temperature process (LTP). Cladding tubes with the improved processing have been irradiated in two commercial PWRs (PWR A and PWR B) and in two test reactors (Test Reactor C and Test Reactor D). The irradiation performance of both versions (HTP X5A and LTP X5A) is reviewed in this paper with a primary emphasis on the current LTP cladding. After achieving an intermediate burnup in the range of 48–54 GWd/Metric Ton of Uranium (MTU) in PWR A, the average maximum oxide layer thickness for LTP X5A was about 23 μm or about 27 % lower than the oxide thickness on ZIRLO® clad fuel rods. In addition, the fuel rod axial growth strain for LTP X5A was about 50 % of ZIRLO rod growth. Lead fuel rod irradiation of LTP X5A in PWR B with burnup in the range of 47–53 GWd/MTU showed about 30 % lower corrosion relative to ZIRLO rods and a 7 % lower axial rod growth strain than ZIRLO. An irradiation experiment in Test Reactor C was designed to study breakaway irradiation growth (in the absence of waterside oxidation) of several alloys. LTP X5A cladding showed a growth strain of about 20 % that of ZIRLO cladding at a fluence of 16×1025n/m2. In Test Reactor D, at a burnup of about 44 GWd/MTU, HTP X5A had the same oxide thickness as ZIRLO. However, the post-irradiation hydrogen pick-up was 35 % lower for HTP X5A compared to ZIRLO. In addition to the irradiation experience, the supplemental out-reactor autoclave evaluation of X5A welds indicates adequate weld corrosion resistance. While additional in-PWR exposures are required along with post-irradiation examination, the results to date demonstrate that X5A is a promising alloy for future PWR application.
Task-phase-specific dynamics of basal forebrain neuronal ensembles (2014)

Cortically projecting basal forebrain neurons play a critical role in learning and attention, and their degeneration accompanies age-related impairments in cognition. Despite the impressive anatomical and cell-type complexity of this system, currently available data suggest that basal forebrain neurons lack complexity in their response fields, with activity primarily reflecting only macro-level brain states such as sleep and wake, onset of relevant stimuli and/or reward obtainment. The current study examined the spiking activity of basal forebrain neuron populations across multiple phases of a selective attention task, addressing, in particular, the issue of complexity in ensemble firing patterns across time. Clustering techniques applied to the full population revealed a large number of distinct categories of task-phase-specific activity patterns. Unique population firing-rate vectors defined each task phase and most categories of task-phase-specific firing had counterparts with opposing firing patterns. An analogous set of task-phase-specific firing patterns was also observed in a population of posterior parietal cortex neurons. Thus, consistent with the known anatomical complexity, basal forebrain population dynamics are capable of differentially modulating their cortical targets according to the unique sets of environmental stimuli, motor requirements, and cognitive processes associated with different task phases.
Metallocene/polypropylene structural relationships: Implications on polymerization and stereochemical control mechanisms (1991)

Abstract A series of group 4 metallocenes possessing chiral ligand environments have been characterized and their structures are compared with their propylene polymerizations. Isotactic, syndiotactic, hemiisotactic, and atactic polypropylenes are produced selectively with chiral monoalkyl metallocene cations paired with perfluorotetraphenyl borate; analogous to the results obtained using methylaluminoxane as a cocatalyst. The relative catalyst stereospecificities suggest specific π‐face selectivities and nonbonded contacts. Trends with solvents, temperatures, and catalyst structures are consistent with contact ion pairing for iPr[CpFlu]Zr‐P(+) in liquid propylene. The specificities of differently structured metallocenes imply chain‐migratory monomer addition reactions.
IC-integrated flexible shear-stress sensor skin (2003)

This paper reports the successful development of the first IC-integrated flexible MEMS shear-stress sensor skin. The sensor skin is 1 cm wide, 2 cm long, and 70 μm thick. It contains 16 shear-stress sensors, which are arranged in a 1-D array, with on-skin sensor bias, signal-conditioning, and multiplexing circuitry. We further demonstrated the application of the sensor skin by packaging it on a semicylindrical aluminum block and testing it in a subsonic wind tunnel. In our experiment, the sensor skin has successfully identified both the leading-edge flow separation and stagnation points with the on-skin circuitry. The integration of IC with MEMS sensor skin has significantly simplified implementation procedures and improved system reliability.
The Use of Androgen Receptor Amino/Carboxyl-Terminal Interaction Assays to Investigate Androgen Receptor Gene Mutations in Subjects with Varying Degrees of Androgen Insensitivity (2003)

Five mutations in the ligand-binding domain (LBD) of the human androgen receptor (hAR) found in patients with varying degrees of androgen insensitivity syndrome (AIS) were investigated for their effects on receptor dynamics. These were Arg(871)Gly (mild), Ser(814)Asn (partial), Glu(772)Ala (partial), Val(866)Met (complete), and Arg(774)Cys (complete). Previous analysis showed that the mutant receptors exhibited near-normal kinetics, except Arg(774)Cys, which had severely reduced androgen binding, and Val(866)Met, which showed increased equilibrium dissociation constant (K(d)) and elevated dissociation rate (k) values. Ser(814)Asn exhibited ligand-selective k values, i.e. increased for dihydrotestosterone and mibolerone, but normal for methyltrenolene. Using mammalian two-hybrid assays, hAR amino/carboxyl (N/C)-terminal interactions of the mutant receptors were analyzed in the presence and absence of the hAR coactivator transcription intermediary factor 2 (TIF2). The mutations conferred decreased hAR N/C-terminal interaction, i.e. mild (approximately 1.5-fold), partial (2-fold), and complete (10-fold), that mirrored the degree of AIS. All mutant LBDs showed a 2- to 3-fold increase in N/C-terminal interactions when TIF2 was cotransfected, although of a magnitude still less than that of wild-type LBD with TIF2. The ligand-selective properties of the Ser(814)Asn mutant were also clearly reflected by the N/C-terminal interactions. Thus, measurement of N/C-terminal interactions may assist in the molecular analysis of mutant hARs associated with AIS.
Vitamin A deficiency and sensory function. (1985)

Morphological investigation of tongue, olfactory epithelia, trachea and inner ear in vitamin A deficiency are reported. The results support assumptions concerning the loss of sensory function as been at least a secondary effect of alterations of the neighbourhood of the sensory cells caused by vitamin A deficiency. Taste buds are hindered in function by a dense layer of squamous cells and olfaction is decreased by atrophy of the surrounding respiratory epithelium. Inner ear functionality seems to be affected by vitamin A status via a stabilizing effect on the endolymph-perilymph barrier.
Rapid and Unidirectional Perforin Pore Delivery at the Cytotoxic Immune Synapse (2013)

The effective engagement of cytotoxic lymphocytes (CLs) with their target cells is essential for the removal of virus-infected and malignant cells from the body. The spatiotemporal properties that define CL engagement and killing of target cells remain largely uncharacterized due to a lack of biological reporters. We have used a novel live cell microscopy technique to visualize the engagement of primary human and mouse CL with their targets and the subsequent delivery of the lethal hit. Extensive quantitative real-time analysis of individual effector-target cell conjugates demonstrated that a single effector calcium flux event was sufficient for the degranulation of human CLs, resulting in the breach of the target cell membrane by perforin within 65-100 s. In contrast, mouse CLs demonstrated distinct calcium signaling profiles leading to degranulation: whereas mouse NKs required a single calcium flux event, CD8(+) T cells typically required several calcium flux events before perforin delivery. Irrespective of their signaling profile, every target cell that was damaged by perforin died by apoptosis. To our knowledge, we demonstrate for the first time that perforin pore delivery is unidirectional, occurring exclusively on the target cell membrane, but sparing the killer cell. Despite this, the CTL membrane was not intrinsically perforin resistant, as intact CTLs presented as targets to effector CTLs were capable of being killed by perforin-dependent mechanisms. Our results highlight the remarkable efficiency and specificity of perforin pore delivery by CLs.
Nanotechnology for wireless gas sensor (2017)

Gas sensor are increasingly being employed, not only in industrial settings and also in every aspect of human life, where they are telling something about their environment by bridging them with the electronic world. Sensors are used to gather a wealth of information from the process that can improve operational efficiency and product quality which involving a mass of data that need to be analysis especially with the merging of the Internet of Things (IoT). They included with new features such as communication capability and on-board diagnostics. Meanwhile, leading edge research in sensors has been propelled by the advancements made in fabrication, signal processing and nanotechnology in the last decade. With these the scientific world is now on the verge of delivering sensors with radically new capabilities for the human societies. This talk deals with the future trend in sensor technology especially for wireless gas sensor and several researches works in Universiti Putra Malaysia related with them.
Infrared absorption in silicon at elevated temperatures (1996)

Isothermal electron beam heating combined with in situ optical measurements has been used to measure the temperature dependence of the spectral emissivity of lightly doped silicon in the range 345–723 °C for wavelengths between 1 and 9 μm. The absorption coefficient was deduced from the spectral emissivity and compared with the predictions of a model including phonon-assisted processes involved in interband transitions, free-carrier absorption, and lattice absorption. The experimental data agree well with the model’s results.
Protease signaling in animal and plant‐regulated cell death (2015)

This review aims to highlight the proteases required for regulated cell death mechanisms in animals and plants. The aim is to be incisive, and not inclusive of all the animal proteases that have been implicated in various publications. The review also aims to focus on instances when several publications from disparate groups have demonstrated the involvement of an animal protease, and also when there is substantial biochemical, mechanistic and genetic evidence. In doing so, the literature can be culled to a handful of proteases, covering most of the known regulated cell death mechanisms: apoptosis, regulated necrosis, necroptosis, pyroptosis and NETosis in animals. In plants, the literature is younger and not as extensive as for mammals, although the molecular drivers of vacuolar death, necrosis and the hypersensitive response in plants are becoming clearer. Each of these death mechanisms has at least one proteolytic component that plays a major role in controlling the pathway, and sometimes they combine in networks to regulate cell death/survival decision nodes. Some similarities are found among animal and plant cell death proteases but, overall, the pathways that they govern are kingdom-specific with very little overlap.
Traffic Classification for the Detection of Anonymous Web Proxy Routing (2015)

There is an increasing need to be able to classify whether an incoming packet is from a legitimate originating IP address or has been modified through an intermediate proxy or node. Being able to verify the originating IP address allows a business (e.g. bank) to use geolocation services in order to then ascertain which geographical location that packet was sent from. This can then feed into the system intrusion system or backend fraud alert mechanisms. The web however is going 'dark'. There is a noticeable uptake in the amount of encrypted data and third party anonymous traffic proxies which aim to mask the try location and IP address of a web request. We present here a system which identifies the characteristics or signatures whenever a user is using a web proxy by developing a Detection System that records packets and analyses them looking for identifying patterns of web proxies.
Dot Off Dot Screen Printing with RGBW Reflective Inks (2023)

Recent advances in pigment production resulted in the possibility to print with RGBW primaries instead of CMYK and performing additive color mixing in printing. The RGBW pigments studied in this work have the properties of structural colors, as the primary colors are a result of interference in a thin film coating of mica pigments. In this work, we investigate the angle-dependent gamut of RGBW primaries. We have elucidated optimal angles of illumination and observation for each primary ink and found the optimal angle of observation under diffuse illumination. We investigated dot off dot halftoned screen printing with RGBW inks on black paper and in terms of angle-dependent dot gain. Based on our observations, optimal viewing condition for the given RGBW inks is in a direction of around 30&#x2218; to the surface normal. Here, the appearance of the resulting halftoned prints can be estimated well by Neugebauer formula (weighted averaging of the individual reflected spectra). Despite the negative physical dot gain during the dot off dot printing, we observe angularly dependent positive optical dot gain for halftoned prints. Application of interference RGBW pigments in 2.5D and 3D printing is not fully explored due to the technological limitations. In this work, we provide colorimetric data for efficient application of the angle-dependent properties of such pigments in practical applications.
Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells (2011)

Sequence-specific gene silencing by short hairpin (sh) RNAs has recently emerged as an indispensable tool for understanding gene function and a promising avenue for drug discovery. However, a wider biomedical use of this approach is hindered by the lack of straightforward methods for achieving uniform expression of shRNAs in mammalian cell cultures. Here we report a high-efficiency and low-background (HILO) recombination-mediated cassette exchange (RMCE) technology that yields virtually homogeneous cell pools containing doxycycline-inducible shRNA elements in a matter of days and with minimal efforts. To ensure immediate utility of this approach for a wider research community, we modified 11 commonly used human (A549, HT1080, HEK293T, HeLa, HeLa-S3, and U2OS) and mouse (CAD, L929, N2a, NIH 3T3, and P19) cell lines to be compatible with the HILO-RMCE process. Because of its technical simplicity and cost efficiency, the technology will be advantageous for both low- and high-throughput shRNA experiments. We also provide evidence that HILO-RMCE will facilitate a wider range of molecular and cell biology applications by allowing one to rapidly engineer cell populations expressing essentially any transgene of interest.
Cognitive base station design for efficient spectrum utilization in cellular network (2014)

This paper proposes the architecture of a cognitive base station for 4G cellular communication networks. We have developed a novel priority driven spectrum handoff technique for Multi-User MIMO communication, where the base station creates beams for establishing communication links. Adaptive beamforming methods along with DoA have been used to dynamically create a beam toward the intended receiver as well as track the users and steer the beam when users are mobile. The work is followed by related simulations which shows 5.4 dB gain over the traditional cellular architecture with respect to CCI. Our proposed algorithms lead to significant increase in overall capacity of the network. It also promises higher spectrum efficiency compared to the traditional cellular architecture.
RDE Treatment Prevents Non-Specific Detection of SARS-CoV-2- and Influenza-Specific IgG Antibodies in Heat-Inactivated Serum Samples (2023)

Assessing the levels of serum IgG antibodies is widely used to measure immunity to influenza and the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) after natural infection or vaccination with specific vaccines, as well as to study immune responses to these viruses in animal models. For safety reasons, sometimes serum specimens collected from infected individuals are subjected to heat inactivation at 56 °C to reduce the risk of infecting personnel during serological studies. However, this procedure may affect the level of virus-specific antibodies, making the results of antibody immunoassays uninterpretable. Here, we evaluated the effect of the heat inactivation of human, ferret and hamster serum samples on the binding of IgG antibodies to the influenza and SARS-CoV-2 antigens. For this, serum samples of naive and immune hosts were analyzed in three variants: (i) untreated sera, (ii) heated at 56 °C for 1 h, and (iii) treated with receptor-destroying enzyme (RDE). The samples were studied through an in-house enzyme-linked immunosorbent assay (ELISA) using whole influenza virus or recombinant proteins corresponding to nucleocapsid (N) protein and the receptor-binding domain of SARS-CoV-2 Spike (RBD) as antigens. We demonstrated that the heat inactivation of the naive serum samples of various hosts can lead to false-positive results, while RDE treatment abolished the effect of the non-specific binding of IgG antibodies to the viral antigens. Furthermore, RDE also significantly decreased the level of virus-specific IgG antibodies in SARS-CoV-2 and influenza-immune sera of humans and animals, although it is unknown whether it actually removes true virus-specific IgG antibodies or only non-specifically binding artifacts. Nevertheless, we suggest that the RDE treatment of human and animal sera may be useful in preventing false-positive results in various immunoassays, while also neutralizing infectious virus, since the standard protocol for the use of RDE also includes heating the sample at 56 °C.
Reverse antenna effect due to process-induced quasi-breakdown of gate oxide (2002)

It was found that wafer charging during processing can cause the quasi-breakdown of gate oxide and lead to more than four orders of magnitude increase in gate leakage current. Gate oxides that have experienced this quasi-breakdown display minimal transistor parameter shifts under constant gate current stress because the stress field sustained across the oxide is low. As a result, large antenna devices, which experience significant wafer charging, have a greater percentage of oxides which have experienced quasi-breakdown and therefore display smaller than expected parameter shifts. To avoid misleading conclusions, an appropriate interpretation of the characterization results is necessary especially for thin gate oxides.
Rdf2Vec Dbpedia Object Frequency Embeddings (2017)

This dataset contains the vectors from computing RDF2vec embeddings from a object frequency weighted DBpedia 2016-04 graph. For each entity in the graph, the text file in the zip archive contains a line with the entity name and the embedded vector. The parameter settings for the embedding are as specified in the paper: Michael Cochez, Petar Ristoski, Simone Paolo Ponzetto, and Heiko Paulheim. 2017. Biased graph walks for RDF graph embeddings. In Proceedings of the 7th International Conference on Web Intelligence, Mining and Semantics (WIMS '17). ACM, New York, NY, USA, Article 21, 12 pages. DOI: https://doi.org/10.1145/3102254.3102279