gebhart/patrepeval huggingface dataset:
RGS Protein Specificity Towards Gq- and Gi/o-Mediated ERK 1/2 and Akt Activation, in vitro (2007)

Extracellular Regulated Kinases (ERK) and Protein Kinase B (Akt) are intermediaries in relaying extracellular growth signals to intracellular targets. Each pathway can become activated upon stimulation of G protein-coupled receptors mediated by G(q) and G(i/o) proteins subjected to regulation by RGS proteins. The goal of the study was to delineate the specificity in which cardiac RGS proteins modulate G(q)and G(i/o)-induced ERK and Akt phosphorylation. To isolate G(q)- and G(i/o)-mediated effects, we exclusively expressed muscarinic M(2) or M(3) receptors in COS-7 cells. Western blot analyses demonstrated increase of phosphorylation of ERK 1.7-/3.3-fold and Akt 2.4-/6-fold in M(2)-/M(3)- expressing cells through carbachol stimulation. In co-expressions, M(3)/G(q)-induced activation of Akt was exclusively blunted through RGS3s/RGS3, whereas activation of ERK was inhibited additionally through RGS2/RGS5. M(2)/G(i/o) induced Akt activation was inhibited by all RGS proteins tested. RGS2 had no effect on M(2)/G(i/o)-induced ERK activation. The high degree of specificity in RGS proteins-depending modulation of G(q)- and G(i/o)-mediated ERK and Akt activation in the muscarinic network cannot merely be attributed exclusively to RGS protein selectivity towards G(q) or G(i/o) proteins. Counter-regulatory mechanisms and inter-signaling cross-talk may alter the sensitivity of GPCR-induced ERK and Akt activation to RGS protein regulation.
Ion Exchange (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIon ExchangeRobert. Kunin, Francis X. McGarvey, and Doris. ZobianCite this: Anal. Chem. 1958, 30, 4, 681–686Publication Date (Print):April 1, 1958Publication History Published online1 May 2002Published inissue 1 April 1958https://pubs.acs.org/doi/10.1021/ac50163a018https://doi.org/10.1021/ac50163a018research-articleACS PublicationsRequest reuse permissionsArticle Views80Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A case of eosinophilic chronic rhinosinusitis associated with optic neuropathy (2011)

We report a case of eosinophilic chronic rhinosinusitis (ECRS) associated with optic neuropathy. The visual acuity in the right eye was suddenly reduced to no light perception on awakening in the morning. Fundus examination of both eyes on the same day showed no remarkable changes. Emergency computed tomography showed pan-sinusitis bilaterally and a partial defect of the sphenoid bone on the right side. From the clinical findings, the case was diagnosed as optic neuropathy associated with chronic sinusitis. Endoscopic sinus surgery (ESS) was performed on the same day, and all of the major sinuses were found to be filled with highly viscous fluid. Part of the optic canal had a defect probably due to inflammatory invasion from the adjacent sphenoid bone. Steroid therapy was started immediately postoperatively. Histopathological examination of excised polyps showed that numerous eosinophils had invaded the polyps but no hyphae were present. The patient reported that he had bronchial asthma and had had nasal polypectomy. Six months after the ESS and steroid therapy, the patient had a recurrence of the sinusitis. At that time, laboratory examination showed an elevation of total IgE and eosinophil numbers. From the clinical findings and course, this case was diagnosed as ECRS accompanied by optic neuropathy. Although ECRS rarely has ocular complications, the inflammation can spread and the optic nerve can be affected.
Development of polyol method for the synthesis of concentrated colloids of PVP-stabilised Ru nanoparticles (2016)

In the current work narrowly distributed and stable Ru nanoparticles (NPs) with mean diameters within 1.7 to 2.8 nm interval were prepared by reduction of RuCl3 with ethylene glycol (EG) as a solvent and a reducing agent as well as polyvinylpyrrolidone (PVP) as a stabiliser in the temperature range from 170°C to 198°C. Besides a typical polyol technique, Ru NPs synthesis using microwaves as well as sodium borohydride reduction at room temperature was applied as an effective alternative approach. The effect of heating conditions, Ru/PVP ratio, reducing agent and metal precursor concentrations on the particle size was studied. Microstructure of Ru NPs was characterised by UV Vis, XPS and HRTEM. Higher PVP/Ru ratios and reduction temperature gave slightly smaller nanoparticles with narrower size distributions, while no clear effect was observed when the initial ruthenium concentration was varied.
Mycobacterium-induced infectious granuloma in Xenopus: histopathology and transmissibility. (1988)

The acid-fast bacteria present in the transmissible putative Xenopus lymphosarcoma (px1) (M. Balls, Cancer Res., 22: 1142-1154, 1962) were isolated and identified as Mycobacterium marinum. M. marinum induces an infectious granuloma in Xenopus which is histopathologically indistinguishable from Xenopus "lymphosarcoma" (px1). It had been reported that the filtrate (0.22-micron pore size) and the supernate of ultracentrifugation (20,000 x g) of the pxl tissue homogenate were bacteria free and could transmit the disease (R.H. Clothier and M. Balls, Oncology, 28: 445-457, 1973; R.H. Clothier and M. Balls, Oncology, 28: 458-480, 1973). Both features, however, also correspond to the transmission of M. marinum-induced granuloma. The granuloma induced by the ultrafiltrate of granulomatous tissue homogenate may be due to the granulomatogenic component of the bacteria-containing tissue, since no bacteria were detected in such filtrate. The 100,000 x g centrifugation supernate of the same homogenate, however, contains bacteria which induce granuloma. Since no other feature of pxl corresponds to neoplasia, it is concluded that as diagnosed by Dawe [C.J. Dawe, Natl. Cancer Inst. Monogr., 32: 7-28, 1969; C.J. Dawe. In: M. Mizell (ed.), Recent Results in Cancer Research, pp. 429-440. New York: Springer-Verlag, 1969; C.J. Dawe. In: R.M. Dutcher (ed.), Comparative Leukemia Research. Bibliotheca Haematologica pp. 634-637. New York: Karger, 1970], this spontaneous abnormal growth of Xenopus actually is an infectious granuloma.
Optimal entrainment of heterogeneous noisy neurons (2015)

We develop a methodology to design a stimulus optimized to entrain nonlinear, noisy limit cycle oscillators with uncertain properties. Conditions are derived which guarantee that the stimulus will entrain the oscillators despite these uncertainties. Using these conditions, we develop an energy optimal control strategy to design an efficient entraining stimulus and apply it to numerical models of noisy phase oscillators and to in vitro hippocampal neurons. In both instances, the optimal stimuli outperform other similar but suboptimal entraining stimuli. Because this control strategy explicitly accounts for both noise and inherent uncertainty of model parameters, it could have experimental relevance to neural circuits where robust spike timing plays an important role.
Designing an auditory lab including active acoustics (2014)

LIVELab, a new facility at McMaster University, has been developed in order to help support research in areas such as developing intelligent hearing aids, measuring the physiological impact of media presentations, understanding interactions between musicians, and determining effective sounds for medical and warning systems. This room combines a quiet performance space featuring a low nominal reverberation time with technology including active acoustics, multichannel playback, and integral measurement and instrumentation to create a sonically flexible facility for auditory research. The design of the room and its acoustic variability for supporting these research endeavors will be discussed.
Onset of Electrical Percolation in Onion-Like Carbon/Poly(methyl methacrylate) Composites (2013)

The results of broadband dielectric spectroscopy of onion-like carbon (OLC) based Poly(methyl methacrylate) (PMMA) composites are reported in wide temperature range 300-450 K. The dielectric properties of OLC/PMMA composites are chiefly dependent on the preparation technique, in particular annealing above the glass transition temperature of composite with 20 wt.% of OLC inclusions causes the vanishing of β relaxation, electrical conductivity appearance and onset of percolation. The percolation threshold in OLC/PMMA composites is found to be close to 20 wt.%. Below the percolation threshold the dielectric properties of composites in temperature range 300-420 K are determined mainly by β relaxation. The activation energy of such a relaxation is weakly dependent on OLC concentration.
Identification of AML-1 and the (8;21) Translocation Protein (AML-1/ETO) as Sequence-Specific DNA-Binding Proteins: theruntHomology Domain is Required for DNA Binding and Protein-Protein Interactions (1993)

The AML1 gene on chromosome 21 is disrupted in the (8;21)(q22;q22) translocation associated with acute myelogenous leukemia and encodes a protein with a central 118-amino-acid domain with 69% homology to the Drosophila pair-rule gene, runt. We demonstrate that AML-1 is a DNA-binding protein which specifically interacts with a sequence belonging to the group of enhancer core motifs, TGT/cGGT. Electrophoretic mobility shift analysis of cell extracts identified two AML-1-containing protein-DNA complexes whose electrophoretic mobilities were slower than those of complexes formed with AML-1 produced in vitro. Mixing of in vitro-produced AML-1 with cell extracts prior to gel mobility shift analysis resulted in the formation of higher-order complexes. Deletion mutagenesis of AML-1 revealed that the runt homology domain mediates both sequence-specific DNA binding and protein-protein interactions. The hybrid product, AML-1/ETO, which results from the (8;21) translocation and retains the runt homology domain, both recognizes the AML-1 consensus sequence and interacts with other cellular proteins.
Suppression of hepatic lymphokine-activated killer cell induction by murine kupffer cells and hepatocytes (1990)

Murine lymphokine-activated-killer cell activity was readily induced by culturing spleen cells with 10 U/ml of interleukin-2 for 4 days. In contrast, very little activity was generated under the same culture conditions when nonparenchymal liver cells were used as the responding cells. It was concluded that Kupffer cells produced prostaglandin and interferon alpha/beta, which suppressed lymphokine-activated-killer induction because (a) induction of lymphokine-activated-killer activity from nonparenchymal liver cells was observed in the presence of indomethacin and anti-interferon alpha/beta antibody; (b) when adherent nonparenchymal liver cells, primarily Kupffer cells, were removed, lymphokine-activated-killer activity could be obtained with interleukin-2 alone; (c) coculture of Kupffer cells with nonadherent nonparenchymal liver cells in a two-chambered system inhibited lymphokine-activated killer cell induction in a dose-dependent manner; (d) exogenous prostaglandin E2 and interferon alpha/beta added at the start of culture inhibited interleukin-2-induced cytotoxicity and proliferation, whereas the other major prostaglandin species in the liver, prostaglandin D2, had little effect. These findings are distinctive with Kupffer cells because splenic macrophages did not exert such inhibition in parallel experiments. Moreover, the supernatant collected from the 24-hr culture of nonparenchymal liver cells contained greater than 20-fold more prostaglandin E2 and interferon alpha/beta than that from culture of spleen cells. In subsequent in vivo experiments, when interleukin-2 was given intraperitoneally to mice, the combination of indomethacin and anti-interferon alpha/beta antibody significantly enhanced lymphokine-activated-killer activity recovered from the liver.(ABSTRACT TRUNCATED AT 250 WORDS)
VISUALIZATION OF FLOW PATTERN OF TSUNAMI IN A HARBOR (1999)

Tsunami causes extreme erosion and accretion in the bottom of a harbor.The collision force of the floating objects is also one of the problems in tsunami's disaster.To understand them, hydraulic model experiments are carried out.Flow pattern in a harbor by tsunami are measured by PTV (Particle Tracking Velocimetry), one of the flow visualization methods.Tsunami intrudes in the harbor along the breakwater, and generates a circulation inside.In case of tsunami that has a large wave gradient, the super-critical flow and hydraulic jumps can be seen in the mouth of a harbor.Accuracy of PTV was also verified in the experiment.The velocity by PTV is compared with that by an electromagnetic current meter.Those have quite good agreements except the space where the hydraulic jump exists.
A New Record of Multi-Branching in Date Palm (Phoenix sylvestris L.) in India (2015)

Usually, date palm doesn’t show branching phenomenon in trunk. Even the healthy plant can become dead after cutting its stem. But sometimes, branching can be observed in date palm tree. This article is an evidence of branching in date palm in India. A date palm is observed with seven branches on its crown segment. This is indeed first time observed event and unrecorded phenomenon of multi-branching in date palm (Phoenix sylvestris L.) in Indian circumstances. Author wants to report this record in some appropriate and suitable periodicals as one of his small discoveries.
AmbiguityVis: Visualization of Ambiguity in Graph Layouts (2015)

Node-link diagrams provide an intuitive way to explore networks and have inspired a large number of automated graph layout strategies that optimize aesthetic criteria. However, any particular drawing approach cannot fully satisfy all these criteria simultaneously, producing drawings with visual ambiguities that can impede the understanding of network structure. To bring attention to these potentially problematic areas present in the drawing, this paper presents a technique that highlights common types of visual ambiguities: ambiguous spatial relationships between nodes and edges, visual overlap between community structures, and ambiguity in edge bundling and metanodes. Metrics, including newly proposed metrics for abnormal edge lengths, visual overlap in community structures and node/edge aggregation, are proposed to quantify areas of ambiguity in the drawing. These metrics and others are then displayed using a heatmap-based visualization that provides visual feedback to developers of graph drawing and visualization approaches, allowing them to quickly identify misleading areas. The novel metrics and the heatmap-based visualization allow a user to explore ambiguities in graph layouts from multiple perspectives in order to make reasonable graph layout choices. The effectiveness of the technique is demonstrated through case studies and expert reviews.
Amplification of human genomic DNA sequences with polymerase chain reaction using a single oligonucleotide primer (1999)

We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only one primer and its complement, at the two ends of the PCR product. Although these experiments were performed with primers derived from the sequence of the prostate specific antigen (PSA) gene and the normal epithelial cell-specific 1 gene (NES1), the amplified sequences were novel and had no homology with either PSA or NES1 DNA. While both PSA and NES1 genes reside on chromosome 19q13.3-q13.4, the amplified sequences were found by mapping to reside on chromosome 5q12 and 5p15.1-p15.3, respectively. When we examined the mechanism of amplification by PCR using one primer in these two cases, we found that there was a high homology between the PSA primer or the NES1 primer and the two regions flanking the amplified sequence of chromosome 5q12 or 5p15. This indicated that the single PSA or NES1 primer could anneal on both strands of the DNA of that region, and mediate the exponential amplification. Since this phenomenon occurred to us twice with a limited number of different PCR reactions performed in our laboratory (< 20), we believe that it may represent a common artifact of PCR. Moreover, it appears that the palindromic primer binding sites can anneal to each other forming DNA cruciforms. J. Clin. Lab. Anal. 13:69–74, 1999. © 1999 Wiley-Liss, Inc.
Photodetectors fabricated from rapid-thermal-oxidized porous Si (1993)

A metal-semiconductor-metal (MSM) photoconductor and a p-n photodiode have been fabricated from rapid-thermal-oxidized (RTO) porous Si. The MSM photoconductor achieved 2.8×higher responsivity at 350 nm than a UV-enhanced Si photodiode, and the RTO photodiode exhibited an external quantum efficiency of 75% at 740 nm.
Double exposure using 193nm negative tone photoresist (2007)

Double exposure is one of the promising methods for extending lithographic patterning into the low k<sub>1</sub> regime. In this paper, we demonstrate double patterning of k<sub>1-effective</sub>=0.25 with improved process window using a negative resist. Negative resist (TOK N- series) in combination with a bright field mask is proven to provide a large process window in generating 1:3 = trench:line resist features. By incorporating two etch transfer steps into the hard mask material, frequency doubled patterns could be obtained.
Synthesis, antiinflammatory activity and platelet antiaggregating activity of a new series of beta-aminoxypropionic acids. (1995)

A series of beta-aminoxypropionic acids (AOPAs) had previously been designed and synthesised as analogues of antiinflammatory arylacetic acids (ArAAs) in which the Ar portion is substituted by the (methyleneaminoxy) methyl moiety (C = NOCH2, MAOMM). Most of these AOPAs had exhibited a significant antiinflammatory and antiaggregating activity. This paper reports the synthesis of a new series of beta-aminoxypropionic acids (SAOPAs) which include the saturated (methylaminoxy)methyl moiety (CHNH-OCH2, SMAOMM) in the place of the MAOMM present in AOPAs. The antiinflammatory activity of SAOPAs was evaluated by the carrageenan-induced paw edema method and the antiaggregating activity was evaluated by means of tests using arachidonic acid (AA) and adenosine diphosphate (ADP) as the aggregating agents. Two SAOPAs were evaluated for their capacity to inhibit the cyclooxygenase enzyme by measuring the malondialdehyde (MDA) produced by incubation of sodium arachidonate with platelet-rich plasma (PRP). The pharmacological results showed that the saturation of the iminic double bond led to a reduction or even the disappearance of the antiaggreganting activity, whereas it did not induce any evident changes in the antiinflammatory activity. Theoretical studies were carried out in order to compare the conformation and the molecular reactivity of SAOPAs with those of AOPAs.
Performance Estimation of Photonic Neural Network Accelerator with Magneto-optical Switch Array (2022)

We estimated performance of photonic neural network accelerator with magneto-optical switch array which provides nonvolatile weighting in convolution layer. Power consumption and operation time assuming AlexNet are calculated including modulators and photodetectors.
Restructuring Electrophysiology During the COVID-19 Pandemic: A Practical Guide From a New York City Hospital Network (2020)

The coronavirus disease 2019 crisis is a global pandemic of a novel infectious disease with far-ranging public health implications. With regard to cardiac electrophysiology (EP) services, we discuss the "real-world" challenges and solutions that have been essential for efficient and successful (1) ramping down of standard clinical practice patterns and (2) pivoting of workflow processes to meet the demands of this pandemic. The aims of these recommendations are to outline: (1) essential practical steps to approaching procedures, as well as outpatient and inpatient care of EP patients, with relevant examples, (2) successful strategies to minimize exposure risk to patients and clinical staff while also balancing resource utilization, (3) challenges related to redeployment and restructuring of clinical and support staff, and (4) considerations regarding continued collaboration with clinical and administrative colleagues to implement these changes. While process changes will vary across practices and hospital systems, we believe that these experiences from 4 different EP sections in a large New York City hospital network currently based in the global epicenter of the coronavirus disease 2019 pandemic will prove useful for other EP practices adapting their own practices in preparation for local surges.
Laser megajoule optical components damage threshold measurements with a large-size beamlet laser (Abstract Only) (1999)

Large components are needed to build up the Laser Megajoule which belongs to the future generation of fusion laser. It is essential to determine the laser damage threshold of full-size components with a large-size laser beam, and to compare this value to the threshold obtained on small samples with a small gaussian laser beam. So, we have, in the first place, tested some small samples from the same coating run on REOSC, CEA and LLNL facilities and, in a second series of experiments, we have tested three full-size optical components on the LLNL beamlet laser. Two types of optical components have been tested on the beamlet: - one polarizer: 710 X 400 nm; - two HfO<SUB>2</SUB>/SiO<SUB>2</SUB> mirrors, from the same coating run: 620 X 440 mm. Before beamlet irradiation these components have been continued at 10 ns on the LLNL Plato facility. Concerning the mirrors, two different conditioning procedures have been applied: - on the first mirror a 5-step conditioning procedure; - on the second mirror a 2-step conditioning procedure. Then, each mirror has undergone the same test on the beamlet. In this paper we report the result obtained on the polarizer and the mirrors after beamlet irradiation.