gebhart/patrepeval huggingface dataset:
Synthetic Receptors for the High‐Affinity Recognition of O‐GlcNAc Derivatives (2016)

Abstract The combination of a pyrenyl tetraamine with an isophthaloyl spacer has led to two new water‐soluble carbohydrate receptors (“synthetic lectins”). Both systems show outstanding affinities for derivatives of N ‐acetylglucosamine (GlcNAc) in aqueous solution. One receptor binds the methyl glycoside GlcNAc‐β‐OMe with K a ≈20 000 m −1 , whereas the other one binds an O‐GlcNAcylated peptide with K a ≈70 000 m −1 . These values substantially exceed those usually measured for GlcNAc‐binding lectins. Slow exchange on the NMR timescale enabled structural determinations for several complexes. As expected, the carbohydrate units are sandwiched between the pyrenes, with the alkoxy and NHAc groups emerging at the sides. The high affinity of the GlcNAcyl–peptide complex can be explained by extra‐cavity interactions, raising the possibility of a family of complementary receptors for O‐GlcNAc in different contexts.
Electronic absorption spectra of binuclear rhodium(I) isocyanide complexes. Comparison of ground-state and d.sigma.* .fwdarw. p.sigma. excited-state bond energies (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic absorption spectra of binuclear rhodium(I) isocyanide complexes. Comparison of ground-state and d.sigma.* .fwdarw. p.sigma. excited-state bond energiesSteven F. Rice, Vincent M. Miskowski, and Harry B. GrayCite this: Inorg. Chem. 1988, 27, 26, 4704–4708Publication Date (Print):December 1, 1988Publication History Published online1 May 2002Published inissue 1 December 1988https://doi.org/10.1021/ic00299a006RIGHTS & PERMISSIONSArticle Views181Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (599 KB) Get e-Alerts Get e-Alerts
The use of mechanical analyses, scanning electron microscopy and ultrasonic imaging to study the effects of high‐pressure processing on multilayer films (2003)

Abstract The effects of high‐pressure processing (HPP) on the mechanical and physical characteristics of eight high‐barrier multilayer films were investigated. These films were PET/SiO x /LDPE, PET/Al 2 O 3 /LDPE, PET/PVDC/nylon/HDPE/PP, PE/nylon/EVOH/PE, PE/nylon/PE, metallised PET/EVA/LLDPE, PP/nylon/PP and PET/PVDC/EVA. In addition, PP was evaluated as a monolayer film for comparison purposes. Pouches made from these films were filled with distilled water, sealed, then pressure processed at 600 and 800 MPa for 5, 10 and 20 min at a process temperature of 45 °C. Pouches kept at atmospheric pressure were used as controls. Prior to and after HPP, all films were tested for tensile strength, percentage elongation and modulus of elasticity (at 50 cm min −1 ) and imaged by scanning electron microscopy (SEM) and C‐mode scanning acoustic microscopy (C‐SAM). Results showed no significant changes in tensile strength, elongation and modulus of elasticity of all films after HPP. However, significant physical damage to metallised PET (MET‐PET) was identified by SEM and C‐SAM. Thus it could be concluded that MET‐PET is not suitable for batch‐type high‐pressure‐processed food packaging. It can also be concluded that the other materials investigated during this study are suitable for batch‐type high‐pressure‐processed food packaging. Copyright © 2003 Society of Chemical Industry
Scanning transitiometry (1996)

S. L. Randzio, Chem. Soc. Rev., 1996, 25, 383 DOI: 10.1039/CS9962500383
Icacinlactone H and Icacintrichantholide from the Tuber of Icacina trichantha (2015)

The 17-norpimarane diterpene is a small group of natural products. A new member, icacinlactone H (1), and a new rearranged 17-norpimarane with an unprecedented carbon skeleton, icacintrichantholide (2), were isolated from Icacina trichantha. Their structures including the absolute configuration were elucidated by spectroscopic analysis, single-crystal X-ray diffraction, and electronic circular dichroism analysis. A plausible biogenetic pathway for 1 and 2 is proposed. Both 1 and 2 showed no significant activity against herpes simplex viruses HSV-1 and HSV-2 as well as the Epstein-Barr virus at 50 μM.
Abstract 20466: De-Sumoylation and Phosphorylation of Magi1 Induce Microcirculation Dysfunction via Endothelial P90rsk Activation in Endotoxemia (2014)

Background: In sepsis, intermittent blood flow (a form of disturbed blood flow) occurs along with microcirculatory dysfunction, leading to multiple tissue/organ damage. Membrane associated guanylate kinase-1 (MAGI1) is a p90RSK-binding protein, but the functional significance of this association remains unknown. Methods and Results: When endothelial cells (ECs) were exposed to disturbed flow or thrombin, p90RSK was activated and MAGI1-S741 was phosphorylated. Normally, MAGI1 is SUMOylated at K931 and localized in the membrane/cytosol. However, after disturbed flow or thrombin stimulation, MAGI1 not only was phosphorylated at S741 but also was de-SUMOylated. These post-translational modifications occurred independently, and were significantly inhibited by dominant negative p90RSK adenovirus transduction (Ad-DN-p90RSK). Thrombin-induced NF-κB activation, E-selectin expression, and EC permeability measured by trans-endothelial electric resistance were significantly inhibited in ECs transduced by Ad-DN-p90RSK and Ad-MAGI1-S741A mutant. Expression of a p90RSK-MAGI1 binding inhibitory fragment (MAGI1aa362-543) significantly inhibited thrombin-induced NF-κB activation. In contrast to the MAGI1-S741A phosphorylation site mutant, MAGI1-K931R SUMOylation defect mutant enhanced thrombin-induced NF-κB activation and EC permeability, while MAGI1-SUMO3 chimera expression inhibited it. Together, these results suggest that MAGI1 de-SUMOylation plays a key role in up-regulating both EC inflammation and permeability. We then studied effects of FMK-MEA, a specific p90RSK inhibitor, on capillary perfusion in the cerebral cortex of mice exposed to endotoxin using in vivo 2-photon microscopy. p90RSK inhibition reduced the incidence of intermittent blood flow (disturbed flow) and the number of rolling leukocytes, and restored the velocity of capillary blood flow in endotoxemic mice. Systemic blood pressure, blood oxygenation, heart rate, and respiratory rate were not affected by FMK-MEA. Conclusions: MAGI1-S741 phosphorylation and MAGI1-K931 de-SUMOylation via p90RSK activation are critical in regulating endothelial inflammation and permeability and may play a major role in micro-circulatory dysfunction in sepsis.
Antimutagenic, anti-inflammatory, and antioxidative activities of the juice of Vitis ficifolia var. Ganebu, a woody vine in the grape family, known as Ryukyu-ganebu in Japan (2021)

These results suggest that the active components in ganebu-K juice are not the same as those in yamabudo, and the components in ganebu-K are attractive candidates as chemopreventive agents.
AKAP12, a gene with tumour suppressor properties, is a target of promoter DNA methylation in childhood myeloid malignancies (2007)

A-kinase anchor protein 12 (AKAP12) is a scaffold protein that participates in mitotic regulation and other signalling processes and probably exerts tumour suppressor function. We hypothesized that epigenetic repression of the AKAP12 gene might occur in malignant myeloid disorders. This study demonstrated that the 5' CpG island of AKAP12 was unmethylated in normal haematopoietic progenitors and granulocytes but exhibited profound methylation in Kasumi-1 and SKNO-1 leukaemic myeloblasts. Correspondingly, AKAP12 was expressed in normal progenitors but transcriptionally silent in leukaemic blasts. Re-expression of AKAP12 in Kasumi-1 and SKNO-1 cells was accomplished by treatment with MS275 alone or in combination with zebularine, indicating epigenetic mechanisms of gene repression. AKAP12 hypermethylation was found in one case of refractory anaemia with excess blasts (RAEB) and two cases of acute myeloid leukaemia (AML) in a panel of 21 blood or bone marrow samples from children with malignant myeloid disorders including refractory cytopenia, RAEB, juvenile myelomonocytic leukaemia and AML. While AKAP12 function has not been previously linked to leukaemogenesis, our results raise the possibility that epigenetic silencing of AKAP12 is involved in myeloid malignancies.
Surface modification of polyester by oxygen‐ and nitrogen‐plasma treatment (2008)

Abstract In this paper, we present a study on the surface modification of polyethyleneterephthalate (PET) polymer by plasma treatment. The samples were treated by nitrogen and oxygen plasma for different time periods between 3 and 90 s. The plasma was created by a radio frequency (RF) generator. The gas pressure was fixed at 75 Pa and the discharge power was set to 200 W. The samples were treated in the glow region, where the electrons temperature was about 4 eV, the positive ions density was about 2 × 10 15 m −3 , and the neutral atom density was about 4 × 10 21 m −3 for oxygen and 1 × 10 21 m −3 for nitrogen. The changes in surface morphology were observed by using atomic force microscopy (AFM). Surface wettability was determined by water contact angle measurements while the chemical composition of the surface was analyzed using XPS. The stability of functional groups on the polymer surface treated with plasma was monitored by XPS and wettability measurements in different time intervals. The oxygen‐plasma‐treated samples showed much more pronounced changes in the surface topography compared to those treated by nitrogen plasma. The contact angle of a water drop decreased from 75° for the untreated sample to 20° for oxygen and 25° for nitrogen‐plasma‐treated samples for 3 s. It kept decreasing with treatment time for both plasmas and reached about 10° for nitrogen plasma after 1 min of plasma treatment. For oxygen plasma, however, the contact angle kept decreasing even after a minute of plasma treatment and eventually fell below a few degrees. We found that the water contact angle increased linearly with the O/C ratio or N/C ratio in the case of oxygen or nitrogen plasma, respectively. Ageing effects of the plasma‐treated surface were more pronounced in the first 3 days; however, the surface hydrophilicity was rather stable later. Copyright © 2008 John Wiley &amp; Sons, Ltd.
Site-specific Drug Delivery in the Dog using Flexible Fiberoptic Endoscopy (1998)

The development of a nonsurgical repeatable method of site-specific delivery to the gastrointestinal tract in the canine is described. Studies to characterize and validate this method were performed utilizing propranolol and etodolac due to their well-known pharmacokinetic properties. Using a catheter placed through the auxiliary port of a flexible fiberoptic endoscope, liquid dosage formulations were consistently delivered to the canine stomach, duodenum, ileum, and colon. It was shown that differences in site-specific delivery could be demonstrated with this model. Propranolol tended to have the highest exposure following dosing to the ileum as compared to other sites. The anesthetic regimen used to perform endoscopy affected certain pharmacokinetic parameters of the compounds being tested including decreasing the intrinsic clearance of propranolol. However, since decreased intrinsic clearance should similarly affect AUCo regardless of the site of delivery, this does not preclude site-specific comparisons to be made. Further, no evidence has been reported for the effect of anesthesia on one GI segment but not another. Thus for other compounds, assuming there are either no anesthetic effects on intestinal pharmacokinetic parameters (absorption, intestinal metabolism, etc.) or that they are consistent and uniform throughout the intestinal tract, this model allows comparisons of the exposure following delivery to differing intestinal sites.
Human lymphocyte subpopulations. (1979)

The secretary of Theodore Roosevelt suggests S. Davis contact the local Progressive Headquarters in Spokane, Washington with his question.
Neue Tetrathiocyanato‐Diamin‐Chromiate mit Naphthylaminen (1974)

Abstract Es wurden Substitutionsreaktionen an entwässertem K 3 [Cr(NCS) 6 ] mit α, bzw. β‐Naphthylamin ohne Lösungsmittel durchgeführt. Die Formeln der neuen stellungsisomeren Komplexanionen: [Cr(NCS) 4 (α‐Naphthylamin) 2 ] − und [Cr(NCS) 4 (β‐Naphthylamin) 2 ] − wurden durch doppelte Umsetzungsreaktionen mit Chlorhydraten einiger heterocyclischen N‐Basen und Kobalt(III)‐aminen bestimmt. Einige Strukturfragen konnten durch UV und UR spektroskopische Untersuchungen geklärt werden.
A Dictyostelium mutant deficient in severin, an F-actin fragmenting protein, shows normal motility and chemotaxis. (1989)

A severin deficient mutant of Dictyostelium discoideum has been isolated by the use of colony immunoblotting after chemical mutagenesis. In homogenates of wild-type cells, severin is easily detected as a very active F-actin fragmenting protein. Tests for severin in the mutant, HG1132, included viscometry for the assay of F-actin fragmentation in fractions from DEAE-cellulose columns, labeling of blots with monoclonal and polyclonal antibodies, and immunofluorescent-labeling of cryosections. Severin could not be detected in the mutant using these methods. The mutation in HG1132 is recessive and has been mapped to linkage group VII. The mutant failed to produce the normal severin mRNA, but small amounts of a transcript that was approximately 100 bases larger than the wild-type mRNA were detected in the mutant throughout all stages of development. On the DNA level a new Mbo II restriction site was found in the mutant within the coding region of the severin gene. The severin deficient mutant cells grew at an approximately normal rate, aggregated and formed fruiting bodies with viable spores. By the use of an image processing system, speed of cell movement, turning rates, and precision of chemotactic orientation in a stable gradient of cyclic AMP were quantitated, and no significant differences between wild-type and mutant cells were found. Thus, under the culture conditions used, severin proved to be neither essential for growth of D. discoideum nor for any cell function that is important for aggregation or later development.
Miniature hollow cathode discharges (1993)

Summary form only given, as follows. Reducing the cathode hole diameter to about 100 /spl mu/m makes it possible to extend the pressure range for hollow cathode discharge operation to atmospheric pressure. For a submillimeter hollow cathode discharge in neon (at 100 Torr) it was shown that the flow of material in the cathode transforms the originally, cylindrical cathode hole into a spherical cavity. This self-forming phenomenon leads to an unusually stable and reproducible discharge. Possible applications for miniature hollow cathode discharges and discharge arrays, such as flat panel displays and large area light sources have been considered.
Characterization of a single reporter-gene potency assay for T-cell-dependent bispecific molecules (2019)

T-cell-dependent bispecific antibodies (TDBs) are promising cancer immunotherapies that recruit patients' T cells to kill cancer cells. There are many TDBs in clinical trials, demonstrating their widely recognized therapeutic potential. However, their complex, multi-step mechanism of action (MoA), which includes bispecific antigen binding, T-cell activation, and target-cell killing, presents unique challenges for biological characterization and potency assay selection. Here, we describe the development of a <i>single</i> reporter-gene potency assay for a TDB (TDB1) that is MoA reflective and sensitive to binding of both antigens. Our reporter-gene assay measures T-cell activation using Jurkat cells engineered to express luciferase under the control of an NFkB response element. The potencies of select samples were measured both by this assay and by a flow-cytometry-based cell-killing assay using human lymphocytes as effector cells. Correlating the two sets of potency results clearly establishes our reporter-gene assay as MoA reflective. Furthermore, correlating potencies for the same panel of samples against binding data measured by binding assays for each individual arm demonstrates that the reporter-gene potency assay reflects dual-antigen binding and can detect changes in affinity for either arm. This work demonstrates that one reporter-gene assay can be used to measure the potency of TDB1 while capturing key aspects of its MoA, thus serving as a useful case study of selection and justification of reporter-gene potency assays for TDBs. Furthermore, our strategy of correlating reporter-gene potency, target-cell killing, and antigen binding for each individual arm serves as a useful example of a thorough, holistic approach to biological characterization for TDBs that can be applied to other bispecific molecules.
Energetics of the spin transition inLaCoO3 (1997)

The heat capacity of ${\mathrm{LaCoO}}_{3}$ has been determined from 13 to 1000 K by adiabatic calorimetry. A spin transition, reported to occur between 20 and 100 K, is reflected in the heat capacity as a small, broad hump. A more substantial heat-capacity contribution, of electronic origin also, has its maximum at \ensuremath{\sim}530 K. The excess heat capacity of the lower temperature transition corresponds to an excess entropy of \ensuremath{\sim}4 J/K mol at 200 K. The excess heat capacity and entropy are discussed with reference to several models proposed for the electronic and structural phase transitions in ${\mathrm{LaCoO}}_{3}$. The experimental results comply with a spin transition taking place in more than one stage, due presumably in part to level splitting by spin-orbit coupling.
Differential Approximation and Sprinting for Multi-Priority Big Data\n Engines (2019)

Today's big data clusters based on the MapReduce paradigm are capable of\nexecuting analysis jobs with multiple priorities, providing differential\nlatency guarantees. Traces from production systems show that the latency\nadvantage of high-priority jobs comes at the cost of severe latency degradation\nof low-priority jobs as well as daunting resource waste caused by repetitive\neviction and re-execution of low-priority jobs. We advocate a new resource\nmanagement design that exploits the idea of differential approximation and\nsprinting. The unique combination of approximation and sprinting avoids the\neviction of low-priority jobs and its consequent latency degradation and\nresource waste. To this end, we designed, implemented and evaluated DiAS, an\nextension of the Spark processing engine to support deflate jobs by dropping\ntasks and to sprint jobs. Our experiments on scenarios with two and three\npriority classes indicate that DiAS achieves up to 90% and 60% latency\nreduction for low- and high-priority jobs, respectively. DiAS not only\neliminates resource waste but also (surprisingly) lowers energy consumption up\nto 30% at only a marginal accuracy loss for low-priority jobs.\n
Lyn Dissociation from Phosphorylated FcεRI Subunits: A New Regulatory Step in the FcεRI Signaling Cascade Revealed by Studies of FcεRI Dimer Signaling Activity (1999)

Abstract Cross-linking the heterotrimeric (αβγ2) IgE receptor, FcεRI, of mast cells activates two tyrosine kinases: Lyn, which phosphorylates β and γ subunit immunoreceptor tyrosine-based activation motifs, and Syk, which binds γ-phospho-immunoreceptor tyrosine-based activation motifs and initiates cellular responses. We studied three FcεRI-dimerizing mAbs that maintain similar dispersed distributions over the surface of RBL-2H3 mast cells but elicit very different signaling responses. Specifically, mAb H10 receptor dimers induce very little inositol 1,4,5-trisphosphate synthesis, Ca2+ mobilization, secretion, spreading, ruffling, and actin plaque assembly, whereas dimers generated with the other anti-FcεRI mAbs induce responses that are only modestly lower than that to multivalent Ag. H10 receptor dimers activate Lyn and support FcεRI β and γ subunit phosphorylation but are poor Syk activators compared with Ag and the other anti-FcεRI mAbs. H10 receptor dimers have two other distinguishing features. First, they induce stable complexes between activated Lyn and receptor subunits. Second, the predominant Lyn-binding phospho-β isoform found in mAb H10-treated cells is a less tyrosine phosphorylated, more electrophoretically mobile species than the predominant isoform in Ag-treated cells that does not coprecipitate with Lyn. These studies implicate Lyn dissociation from highly phosphorylated receptor subunits as a new regulatory step in the FcεRI signaling cascade required for Syk activation and signal progression.
CRM1 and its ribosome export adaptor NMD3 localize to the nucleolus and affect rRNA synthesis (2013)

CRM1 is an export factor that together with its adaptor NMD3 transports numerous cargo molecules from the nucleus to cytoplasm through the nuclear pore. Previous studies have suggested that CRM1 and NMD3 are detected in the nucleolus. However, their localization with subnucleolar domains or participation in the activities of the nucleolus are unclear. We demonstrate here biochemically and using imaging analyses that CRM1 and NMD3 co-localize with nucleolar marker proteins in the nucleolus. In particular, their nucleolar localization is markedly increased by inhibition of RNA polymerase I (Pol I) transcription by actinomycin D or by silencing Pol I catalytic subunit, RPA194. We show that CRM1 nucleolar localization is dependent on its activity and the expression of NMD3, whereas NMD3 nucleolar localization is independent of CRM1. This suggests that NMD3 provides nucleolar tethering of CRM1. While inhibition of CRM1 by leptomycin B inhibited processing of 28S ribosomal (r) RNA, depletion of NMD3 did not, suggesting that their effects on 28S rRNA processing are distinct. Markedly, depletion of NMD3 and inhibition of CRM1 reduced the rate of pre-47S rRNA synthesis. However, their inactivation did not lead to nucleolar disintegration, a hallmark of Pol I transcription stress, suggesting that they do not directly regulate transcription. These results indicate that CRM1 and NMD3 have complex functions in pathways that couple rRNA synthetic and processing engines and that the rRNA synthesis rate may be adjusted according to proficiency in rRNA processing and export.