gebhart/patrepeval huggingface dataset:
Tricarbonylmanganese(i)–lysozyme complex: a structurally characterized organometallic protein (2007)

The reaction of the new and structurally characterized covalent {Mn(CO)(3)(H(2)O)(2)}(+)-lysozyme adduct with NiS(4) and NiN(2)S(2) complexes generates binuclear Ni-Mn complexes; relevance to the reactivity of the protein-bound {Fe(CO)(CN)(2)} intermediate during maturation of [NiFe] hydrogenases is discussed.
Direct micro-pattern machining of metal molds using pico-second lasers (2010)

As the new method of machining, laser ablation has a lot of attractive advantages. The possibilities of dead end engraving and stable long term machining would be the great merits of micro-patterning to large metal mold which is used to the optical sheets for flat panel display for example. We developed pico-second laser machining system and manufactured micro-patterns on metal mold surface. And from those sample-machining results, we concluded it is possible to adapt this system to metal mold micro-pattern machining and decided to continue our development of laser machining.
Nociception in chicken embryos, Part III: Analysis of movements before and after application of a noxious stimulus (2023)

Abstract Many potentially noxious interventions are performed on chicken embryos in research and in the poultry industry. It is therefore in the interest of animal welfare to define the point at which a chicken embryo is capable of nociception. The present part III of a comprehensive study examined the movements of developing chicken embryos with the aim of identifying behavioral responses to aww noxious stimulus. For this purpose, a noxious mechanical stimulus and a control stimulus were applied. The recorded movements of the embryos were evaluated using the markerless pose estimation software DeepLabCut and manual observations. After the application of the noxious stimulus, a significant increase in beak movement was identified in 15- to 18-day-old embryos. In younger embryos, no behavioral changes related to the noxious stimulus were observed. The results indicate that noxious stimuli at the beak base evoke a nocifensive reaction in chicken embryos starting at embryonic day 15.
OPTIMIZATIONPHILOSOPHY,DESIGNAND TESTRESULTS (1978)

A methodbasedon dynamicprogramming was developed to design optimized post-type spacersforthree-phase, sulfur hexafluoride gas insulated cables. Thispaper describes theoptimization philosophy, itsimplementation to thedesignof experimental spacers,andtheresults ofimpulse voltage tests.
Study of Bend to Twist Coupling of Composite Laminates for Passive Load Alleviation of a Wind Turbine Blade (2018)

One of the biggest challenges wind turbines in low speed wind regions (sudden extreme wind speeds) face, is bearing the sudden and excessive loads caused by typhoons and tropical cyclones in the region. The extreme turbulence and rapid change in the wind direction and speeds are the major causes of wind turbine failures. This leads to blade pitch angle error in the traditional pitching mechanism used for load alleviation, causing large responses in terms of rotor thrust and generator torque. This can potentially lead to fatal accidents. Hence, passive load alleviation techniques are gaining ground, bend to twist coupling of anisotropic composite laminates in particular. The induced twist when the laminate is under bending loads helps reduce excessive loads in the structure. This concept can be applied to the blades of the stalled wind turbines to withstand extreme loads. Previous research findings support this hypothesis and show that eliminating the pitching subsystems can help create lighter blades that require less maintenance. In this study, five single-ply and five two-ply laminates are analysed using a MATLAB program to determine their bending-twisting coupling coefficients, strains and stresses under an applied moment. For our loading condition, the study seems to indicate that the addition of the 25° ply is optimal in terms of reducing the stresses by twisting. Further work is required in order to understand the effect of the number of plies, ply architecture, different cross-sections, etc. on the bend-twist coupling of composite structures so as to incorporate it into a wind turbine blade spar.
Extended Second Price Auctions With Elastic Supply for PEV Charging in the Smart Grid (2016)

In this paper, we explore the question of efficient allocation of energy, while buying the same from generation companies, to plug-in electric vehicles (PEVs) by aggregator (electricity utility or load serving entities) through auction mechanisms. Recognizing the practical limitations of the Vickrey–Clarke–Groves mechanism, which would be natural to apply in this context, we investigate two practical mechanisms that can be viewed as extensions of second price auction mechanisms and have limited message (bid) complexity. In the first mechanism, the elastic-supply multi-level second price, each PEV agent submits a number of price bids, one for each of a given set of energy levels (energy quantities). In the second mechanism, the elastic-supply progressive second price, the PEV agents submit a 2-D bid indicating the price as well as the desired energy quantity. Taking into account differences across PEV-owners in terms of their willingness-to-pay values and charging time constraints, we analyze the social optimality and incentive compatibility properties of the two auction mechanisms. We also complement our theoretical findings with numerical simulations.
Stability Analysis and Design of Composite Structures (2015)

The direct analysis method is the primary means of assessing system stability within a standard specification. This method, and in particular its use of reduced stiffness, has been thoroughly validated for use in frames consisting of structural steel members. However, appropriate stiffness reductions have not yet been established nor has the method as a whole been validated for frames with steel-concrete composite columns. Through comparisons between second-order inelastic analysis results and results from the design methodology on a parametric suite of small frames, the current design provisions are evaluated in this paper. The results indicate that while the current design provisions are safe and accurate for the majority of common cases, there exist cases in which the current provisions result in high levels of unconservative error. Modifications to the current design provisions are proposed to address these issues.
Effect of TRH on Phospholipase D Activity in GH3 Cell (2002)

Background: G cell are a well characterized and widely used model used for the in vitro study of growth hormone (GH) secretion. Thyrotropin releasing hormone(TRH) binds to receptors belonging to the family of G protein-coupled receptors, and secrets both GH & prolactin. Phopholipase D (PLD) is and enzyme that hydrolyses phosphatidylcholine to yield phosphatidic acid and choline, and plays important roles in celluar proliferation and hormonal secretion. To elucidate the pathway of the action of TRH in G cell, we investigated the activities of PLC and PLD in G cell treated with TRH or phorbor 12-myristate 13-acetate (PMA). Methods: G cells were labeled with []myristate, followed by incubation of with 0.3% ethanol, prior to before the addition of the agonists. The total lipids were extracted from the harvested cells following treatment with the agonists. The PLD activity was assessed by mearsuring [] Phosphatidylethanol from the [] phospholipid using thin layer chromatography. Results: TRH (1 M) stimulated the PLC activity by 44-fold over that the control values. TRH (1 M), mastoparan (5 M), and PMA (500 M) for 30 minutes increased PLD activity by 1.9, 1.5 and 2.2 fold, respectively, in comparison to the controls. The PLD activities after 15, 30, 60, 120 and 240 min treatments of TRH (1 M) were 142%, 170%, 172%, 160% and 115%, respectively.
Material performance in a solvent extraction Liquefaction plant in Japan (1996)

Abstract Corrosion behaviour of various steels and alloys in the solvent extraction coal liquefaction process in Japan has been studied by the inplant testing in a 1 ton/ day plant and by the laboratory tests related to it. The severest corrosion was observed in the high pressure separator, corrosion thickness loss and pitting depth being the greatest and stress corrosion cracking being observed for 18–8 series austenitic stainless steels. The cause may be the occurrence of water condensate containing high Cl − and H 2 S. Otherwise corrosion was relatively mild. No obvious symptom of notorious organic‐chloride corrosion reported in the U.S. has been observed.
A Measurement System for Human Movement Analysis (2012)

Parkinson's disease is a degenerative disorder of the central nervous system which significantly deteriorates the quality of life especially in the elderly.The prognosis of the disease includes impairment of movement related motions e.g. the gait, shaking of hands (tremor), rigidity, bradykinesia and akinesia, and postural instability etc.The work included in this thesis focuses on developing a system to measure the degree of impairment with patients having this problem.The developed prototype has six degrees of freedom and is an inertial measurement unit to measure and quantify body movements, e.g.Tremor, which is the most well established symptom of this disease.The developed prototype was evaluated and the data acquired from the motion tests was displayed graphically through MATLAB.The prototype showed satisfactory performance and has potential to emerge as a tool in diagnosing and predicting the pace of the disease and a possible feedback system for rehabilitation of patients suffering with Parkinson Disease.
Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta. (1996)

The alpha subunit of the karyopherin heterodimer functions in recognition of the protein import substrate and the beta subunit serves to dock the trimeric complex to one of many sites on nuclear pore complex fibers. The small GTPase Ran and the Ran interactive protein, p10, function in the release of the docked complex. Repeated cycles of docking and release are thought to concentrate the transport substrate for subsequent diffusion into the nucleus. Ran-GTP dissociates the karyopherin heterodimer and forms a stoichiometric complex with Ran-GTP. Here we report the mapping of karyopherin beta's binding sites both for Ran-GTP and for karyopherin alpha. We discovered that karyopherin beta's binding site for Ran-GTP shows a striking sequence similarity to the cytoplasmic Ran-GTP binding protein, RanBP1. Moreover, we found that Ran-GTP and karyopherin alpha bind to overlapping sites on karyopherin beta. Having a higher affinity to the overlapping site, Ran-GTP displaces karyopherin alpha and binds to karyopherin beta. Competition for overlapping binding sites may be the mechanism by which GTP bound forms of other small GTPases function in corresponding dissociation-association reactions. We also mapped Ran's binding site for karyopherin beta to a cluster of basic residues analogous to those previously shown to constitute karyopherin alpha's binding site to karyopherin beta.
Modeling Noisy Annotated Data with Application to Social Annotation (2013)

We propose a probabilistic topic model for analyzing and extracting content-related annotations from noisy annotated discrete data such as webpages stored using social bookmarking services. With these services, because users can attach annotations freely, some annotations do not describe the semantics of the content, thus they are noisy, i.e., not content related. The extraction of content-related annotations can be used as a prepossessing step in machine learning tasks such as text classification and image recognition, or can improve information retrieval performance. The proposed model is a generative model for content and annotations, in which the annotations are assumed to originate either from topics that generated the content or from a general distribution unrelated to the content. We demonstrate the effectiveness of the proposed method by using synthetic data and real social annotation data for text and images.
A Bit Allocation Method for Sparse Source Coding (2013)

In this paper, we develop an efficient bit allocation strategy for subband-based image coding systems. More specifically, our objective is to design a new optimization algorithm based on a rate-distortion optimality criterion. To this end, we consider the uniform scalar quantization of a class of mixed distributed sources following a Bernoulli-Generalized Gaussian distribution. This model appears to be particularly well-adapted for image data which have a sparse representation in a wavelet basis. In this paper, we propose new approximations of the entropy and the distortion functions by using piecewise affine and exponential forms, respectively. Thanks to these approximations, bit allocation is reformulated as a convex optimization problem. Solving the resulting problem allows us to derive the optimal quantization step for each subband. Experimental results show the benefits that can be drawn from the proposed bit allocation method in a typical transform-based coding application.
Small molecule inhibitors of fatty acid uptake in 3T3‐L1, primary human adipocytes, INS‐1E and C2C12 cell lines (2010)

Previous work in this lab identified 5 chemically distinct small molecules that inhibit the uptake of fatty acid (C1‐BODIPY‐C12) through a series of screenings in yeast expressinghomo sapiens fatty acid transport protein (FATP) 2 and Caco2 cells. Subsequently, we looked to examine the effectiveness and toxicity of each compound in a variety of cells that accumulate lipid. We examined two adipocyte cell lines (mouse 3T3‐L1 and primary human), one pancreatic beta cell line (INS‐1E) and one skeletal muscle cell line (mouse C2C12). Cells were seeded into 96 well (black wall/optically clear bottom) plates and differentiated or allowed to incubate until firmly attached. Cells were subsequently starved for one hour in serum free MEM, one hour incubation with a dilution series of each compound (1nM‐1mM, range dependent on individual cell line), a 5–15 minute incubation with the fluorescent fatty acid C1‐BODIPY‐C12 and finally measured fluorescence intensity using 485/20 nm excitation and 530/20 nm emission filters. Sigmoid dose‐response curves for C1‐BODIPY‐C12uptake were generated in each cell line and EC50 values were from the low nM to high μM range, depending on the particular compound and cell line. The compounds were determined to benon‐toxic to each cell line by utilizing the MTT cytotoxicity assay. Specificity to fatty acid uptake was confirmed by measuring acyl‐CoA synthestase activity and glucose uptake. [Supported by NIH DK071076 ]
Escherichia coli Bacteremia in Children (2014)

E. coli strains responsible for bacteremia exhibit specific characteristics according to age of children. However, host characteristics and portal of entry are the main determinants of severity of E. coli bacteremia in children, as observed in adults.
On the Photoabsorption by Permanganate Ions in Vacuo and the Role of a Single Water Molecule. New Experimental Benchmarks for Electronic Structure Theory (2013)

We report electronic spectra of mass-selected MnO4(-) and MnO4(-)⋅H2O using electronic photodissociation spectroscopy. Bare MnO4(-) fragments by formation of MnO3(-) and MnO2(-), while the hydrated complex predominantly decays by loss of the water molecule. The band in the visible spectral region shows a well-resolved vibrational progression consistent with the excitation of a Mn-O stretching mode. The presence of a single water molecule does not significantly perturb the spectrum of MnO4(-). Comparison with the UV/Vis absorption spectrum of permanganate in aqueous solution shows that complete hydration causes a small blueshift, while theoretical models including a dielectric medium have predicted a redshift. The experimental data can be used as benchmarks for electronic structure theory methods, which usually predict electronic spectra in the absence of a chemical environment.
Enantioselective Synthesis of Paraconic Acids. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
CD44 isoform expression in primary and metastatic pancreatic adenocarcinoma. (1995)

CD44 is the transmembrane adhesion molecule which binds hyaluronate. The gene encoding CD44 is found on chromosome 11p and comprises 20 exons. Differential splicing of the 10 extracellular juxtamembranous exons (v1-10) generates the major isoforms of CD44. The major CD44 isoform found on hematopoetic cells (CD44s) contains none of the variably expressed exons, while the major isoform expressed on epithelial cells [CD44(v8-10)] contains exons v8-10. Metastasis-specific isoforms of CD44 were first documented in a model of rat pancreatic adenocarcinoma [CD44(v4-7), CD44(v6-7)] and subsequently in other cancers. This study is the first characterization of CD44 isoforms in primary and metastatic human pancreatic adenocarcinomas. CD44 isoforms were analyzed in specimens of 15 primary and 6 metastatic pancreatic adenocarcinomas as well as in 6 specimens of control pancreata by two different methods. Radiolabeled reverse transcriptase-PCR coupled with 8% PAGE allowed analysis of the major isoforms of CD44, while Southern blot hybridization with [alpha-32P]dCTP-labeled probes permitted analysis for metastasis-specific CD44 isoforms containing CD44(v6) or CD44(v8-10). No differences in the expression of CD44(v8-10) and CD44s were found among the primary and metastatic pancreatic adenocarcinomas, and control specimens of pancreata. However, a novel CD44(v6) isoform was found in metastatic lesions and may represent the human homologue of the rat pancreatic adenocarcinoma metastasis-associated CD44 isoform.
Effect of a novel mucoadhesive polysaccharide obtained from tamarind seeds on the intraocular penetration of gentamicin and ofloxacin in rabbits (2000)

This report describes the efficacy of a novel mucoadhesive polymer, the tamarind seed polysaccharide, as a delivery system for the ocular administration of hydrophilic and hydrophobic antibiotics. Healthy rabbits were subjected to repeated ocular instillations with either conventional gentamicin or ofloxacin or these agents viscosified with the tamarind seed polysaccharide. Administration of viscosified preparations produced antibiotic concentrations both in the aqueous humour and cornea that were significantly higher than those achieved with the drugs alone. The increased drug absorption and the prolonged drug elimination phase obtained with the viscosified formulations indicate the usefulness of the tamarind seed polysaccharide as an ophthalmic delivery system for topical administration of antibiotics.
Machine Learning in Compiler Optimization (2018)

In the last decade, machine-learning-based compilation has moved from an obscure research niche to a mainstream activity. In this paper, we describe the relationship between machine learning and compiler optimization and introduce the main concepts of features, models, training, and deployment. We then provide a comprehensive survey and provide a road map for the wide variety of different research areas. We conclude with a discussion on open issues in the area and potential research directions. This paper provides both an accessible introduction to the fast moving area of machine-learning-based compilation and a detailed bibliography of its main achievements.
Characterizing Triplet States of Quinonoidal Dinitrenes as a Function of Conjugation Length (1997)

Photolysis of para,para‘-diazide precursors in 2-methyltetrahydrofuran gives biradical ESR spectra assignable to quinonoidal dinitrenes with excited triplet biradical states. The zero field splitting (zfs) parameters for the biradicals at 77 K are as follows: 1,4-phenylenedinitrene (|D/hc| = 0.169 cm-1, |E/hc| = 0.004 cm-1); 4,4‘-biphenyldinitrene (|D/hc| = 0.188 cm-1, |E/hc| ≤ 0.002 cm-1); 4,4‘-stilbenedinitrene (|D/hc| = 0.122 cm-1, |E/hc| < 0.002 cm-1); 1,4-bis(p-nitrenophenyl)buta-1,3-diene (|D/hc| = 0.0865 cm-1, |E/hc| < 0.002 cm-1); 1,8-bis(p-nitrenophenyl)octa-1,3,5,7-tetraene (|D/hc| = 0.0442 cm-1, |E/hc| < 0.001 cm-1). These zfs parameters are inconsistent with simple dipole−dipole analysis of the ESR spectra in localized biradicals, but are reconcilable with a spin-polarized model involving the π-electrons of the biradicals.
The integration of LC‐MS and NMR for the analysis of low molecular weight trace analytes in complex matrices (2018)

This review discusses the integration of liquid chromatography (LC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) in the comprehensive analysis of small molecules from complex matrices. We first discuss the steps taken toward making the three technologies compatible, so as to create an efficient analytical platform. The development of online LC-MS-NMR, highlighted by successful applications in the profiling of highly concentrated analytes (LODs 10 μg) is discussed next. This is followed by a detailed overview of the alternative approaches that have been developed to overcome the challenges associated with online LC-MS-NMR that primarily stem from the inherently low sensitivity of NMR. These alternative approaches include the use of stop-flow LC-MS-NMR, loop collection of LC peaks, LC-MS-SPE-NMR, and offline NMR. The potential and limitations of all these approaches is discussed in the context of applications in various fields, including metabolomics and natural product discovery.
THE EFFECT OF BUFFY COAT-POOR BLOOD TRANSFUSION ON SUBSEQUENT HEMOPOIETIC GRAFTS (1973)

SUMMARY Previous studies in dogs have shown that a single preceding transfusion with whole blood from the intended marrow donor can immunize a recipient and lead to rejection of a subsequent marrow graft even when donor and recipient are littermates compatible for the major dog leukocyte antigen (DL-A) locus. The present study investigated two approaches to diminish the chance of marrow graft rejection in transfused canine recipients: (1) the use of the immunosuppressive drug methotrexate (MTX) after grafting, and (2) the use of buffy coat-poor blood instead of whole blood for the preceding transfusion. Recipients were conditioned for marrow grafting by 1,200 R of total body irradiation followed within 4 hr by infusion of hemopoietic cells from DL-A-incompatible unrelated donors. MTX, 0.4–0.5 mg/kg, was administered i.v. on days 1, 3, 6, and 11 after irradiation and grafting and then once weekly until day 102. A single preceding transfusion of whole blood from the intended DL-A-incompatible marrow donor in most instances led to rejection of the marrow graft. Treatment with MTX did not diminish the incidence of graft rejection. Substituting buffy coat-poor blood (reduction in white blood cells: 99.8 ± 0.2% (SD) and platelets: 99.1 ± 0.7% (SD)) for whole blood did not significantly change the incidence of immunization of recipients.