297 ESTABLISHMENT OF A RENAL CELL CARCINOMA CELL LINE ASSOCIATED WITH TFE3 GENE FUSIONS (2013)

You have accessJournal of UrologyKidney Cancer: Basic Research (II)1 Apr 2013297 ESTABLISHMENT OF A RENAL CELL CARCINOMA CELL LINE ASSOCIATED WITH TFE3 GENE FUSIONS Megumi Hirobe, Naoya Masumori, Toshiaki Tanaka, Hiroshi Kitamura, and Taiji Tsukamoto Megumi HirobeMegumi Hirobe Sapporo, Japan More articles by this author , Naoya MasumoriNaoya Masumori Sapporo, Japan More articles by this author , Toshiaki TanakaToshiaki Tanaka Sapporo, Japan More articles by this author , Hiroshi KitamuraHiroshi Kitamura Sapporo, Japan More articles by this author , and Taiji TsukamotoTaiji Tsukamoto Sapporo, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1681AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Xp11.2 translocation renal cell carcinoma is a rare disease diagnosed in children and adolescents in the advanced stage with an aggressive clinical course. It has been recently recognized as a subtype of renal cell carcinoma primarily described in the 2004 World Health Organization classification of renal tumors, and various gene fusions including the transcription factor E3 (TFE3) gene located on chromosome × cause the tumor; however, little is known about the tumor characteristics. We report the cytogenetic and biological characteristics of a renal cell carcinoma cell line with TFE3 gene fusion established from a young female with locally advanced renal cell carcinoma. METHODS Tumor cells were obtained from a renal tumor in a 16-year-old Japanese female. This primary tumor was pathologically diagnosed as renal cell carcinoma with Xp11 translocations. After cultured in vitro, cell suspension was inoculated into the nude mice to establish xenograft. TFE3 immunostaining and other immunohistochemistries were performed. Molecular and cytogenetic methods such as RT-PCR, karyotype analysis and FISH were added. RESULTS The established cell line and its xenograft showed strong nuclear staining for TFE3 in immunohistochemistry, TFE3 gene rearrangement in dual-color, break-apart FISH analysis, and ASPL-TFE3 type 1 fusion transcripts detected by RT-PCR and direct DNA sequencing. Multicolor FISH analysis showed more complicated rearrangements on the × chromosome than expected. CONCLUSIONS Although many renal cell carcinoma cell lines have been established and investigated, only a few cell lines are recognized as Xp11.2 translocation carcinoma. This cell line will be useful to examine the characteristics and drug susceptibility of Xp11 translocation renal cell carcinoma. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e121 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Megumi Hirobe Sapporo, Japan More articles by this author Naoya Masumori Sapporo, Japan More articles by this author Toshiaki Tanaka Sapporo, Japan More articles by this author Hiroshi Kitamura Sapporo, Japan More articles by this author Taiji Tsukamoto Sapporo, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...