gebhart/patrepeval huggingface dataset:
Fluorescence‐based quantitative scratch wound healing assay demonstrating the role of MAPKAPK‐2/3 in fibroblast migration (2009)

The scratch wound healing assay is a sensitive method to characterize cell proliferation and migration, but it is difficult to be quantitatively evaluated. Therefore, we developed an infrared fluorescence detection-based real-time assay for sensitive and accurate quantification of cell migration in vitro. The method offers sensitivity, simplicity, and the potential for integration into automated large-scale screening studies. A live cell staining lipophilic tracer-1,1'-dioctadecyl-3,3,3',3'-tetramethyl indotricarbocyanine iodide (DiR)-is used for accurate imaging of wound closure in a simple 96-well scratch assay. Scratches are made on prestained confluent cell monolayers using a pipette tip and scanned at different time intervals using a fluorescent scanner. Images are analyzed using Image J software and the migration index is calculated. Effect of cell number, time after scratch and software settings are analyzed. The method is validated by showing concentration- and time-dependent effects of cytochalasin-D on fibroblast migration. Using this assay, we quantitatively evaluate the role of the MAPK-activated protein kinases MK2 and MK3 in fibroblast migration. First, the migratory phenotype of MK2-deficient MEFs is analyzed in a retroviral rescue model. In addition, migration of MK2/3-double-deficient cells is determined and the ability of MK3 to rescue cell migration in MK2/3-double-deficient fibroblasts is demonstrated.
Certain Soil Surfactants Could Become a Source of Soil Water Repellency after Repeated Application (2021)

Repeated application of soil surfactants, or wetting agents, is a common practice for alleviating soil water repellency associated with soil organic coatings. However, wetting agents are organic compounds that may also coat soil particle surfaces and reduce wettability. For this experiment, hydrophobic sands from the field and fresh, wettable sands were collected and treated with either a polyoxyalkylene polymer (PoAP) or alkyl block polymer (ABP) wetting agent, or water only treatments served as a control. Following repeated treatment application and sequential washings, dissolved and particulate organic carbon (OC) were detected in the leachates of both sand systems. The total amount of OC recovered in leachates was 88% or less than the OC introduced by the wetting agents, indicating sorption of wetting agent monomers to soil particle surfaces regardless of soil hydrophobicity status. While ABP treatment did not alter solid phase organic carbon (SOC) in the sands studied, PoAP application increased SOC by 16% and 45% which was visible in scanning electronic microscopy images, for hydrophobic and wettable sands, respectively. PoAP application also increased the hydrophobicity of both sands that were studied. In contrast, ABP treatment increased the wettability of hydrophobic sand. Our results provide strong evidence that certain wetting agents may increase soil hydrophobicity and exacerbate wettability challenges if used repeatedly over time.
Audible Noise Reduction in Eigendomain for Speech Enhancement (2007)

A signal subspace scheme based on masking properties is proposed for enhancement of speech degraded by additive noise. Since the masking properties are related to the critical frequency band that is derived from the characteristics of human cochlea, the incorporation of masking threshold into a subspace technique requires the transformation between the frequency and eigen domains. We present and apply an invertible transformation between the frequency and eigen domains. In this paper, we use masking properties of the human auditory system to define the audible noise quantity in the eigendomain. We derive the eigen-decomposition of the estimated speech autocorrelation matrix with the assumption of white noise. Subsequently, an audible noise reduction scheme is developed based on a signal subspace technique, and the implementation of our proposed scheme is outlined. We further extend the scheme to the colored noise case. Simulation results show the superiority of our proposed scheme over other existing subspace methods in terms of segmental signal-to-noise ratio (SNR), perceptual evaluation of speech quality (PESQ), modified Bark spectral distortion (MBSD), spectrogram and informal listening tests.
MicroRNAs in Kidney Transplantation: Living up to Their Expectations? (2015)

Since the discovery of microRNAs, ample research has been conducted to elucidate their involvement in an array of (patho)physiological conditions. Ischemia reperfusion injury is a major problem in kidney transplantation and its mechanism is still not fully known, nor is there an effective therapy. Furthermore, no biomarker is available to specifically measure (ischemic) damage after kidney transplantation or predict transplantation outcome. In this review, we summarize studies conducted on microRNAs in renal ischemia reperfusion injury and kidney transplantation. Although the number of publications on miRNAs in different areas of nephrology is increasing every year, only a limited number of reports that address the role of miRNAs in relation to ischemia reperfusion injury or kidney transplantation are available. All reports up to June 2014 on microRNAs in renal IRI, kidney transplantation, and renal allograft status were included. Design of the studies was highly variable and there was limited overlap between microRNAs found in these reports. No single microRNA expression pattern could be found, although multiple microRNAs involved in the immune response seem to be altered after ischemia reperfusion injury and kidney transplantation. Although there is a growing interest in microRNA research in kidney transplantation aiming to identify biomarkers and therapeutical targets, to date, no specific microRNA has been demonstrated to be applicable as either one, mostly because of lack of specificity. More systematical research is needed to determine whether microRNAs can be applied as biomarker, therapeutic target, or therapeutic agent in kidney transplantation.
Performance of IGBTs Series Connection Technologies for Auxiliary Power Supply System (2007)

This paper presents a novel auxiliary power supply (APS) system for the commuter trains with 1.5kVdc employing series-connected 1.2-kV general purpose IGBTs as a switching device, substituted for high-voltage IGBTs over 3kV. The carrier frequency of 5kHz in the APS is realized due to considerable low switching loss of the IGBTs. Voltage balance across the series connected IGBTs are achieved by magnetically coupling all the gate lines using a simple two-winding transformer without snubber circuits. To ensure that such the high carrier frequency is effective on APS performance, sound level from filter and output voltage stability with a sudden changed load are evaluated using a single-phase inverter, and then compared with that of a conventional carrier frequency. On the basis of the series connection technique with the 1.2kV IGBTs, a prototype of the APS system is designed and built for the actual uses, and then its performance is evaluated at its rating specifications. From the results, it is confirmed that the APS system features smaller volume and lower sound level rather than conventional ones with the high-voltage IGBTs.
Triplanar Humeral Osteotomy for Restoration of Midline Function in Patients With Brachial Plexus Birth Palsy (2017)

Brachial plexus birth palsy resolves spontaneously in a majority of patients, however, others may have serious permanent dysfunction. Although nerve transfers or grafts are early options for treatment, many children have residual deficits or present too late for such procedures. In these patients, rotational osteotomy of the humerus may restore improved function and motion. Unfortunately, traditional humeral osteotomies only provide correction in a single plane, therefore appropriate correction of the typical residual deformity is incomplete. Here, we describe a novel technique for obtaining a calculated correction in 3 planes using a single osteotomy of the humerus on the basis of a mathematical equation. Nine patients are described here with an average of 35.4 months follow-up. Corrections were obtained in adduction, extension, and either internal or external rotation depending on the initial deformity and Modified Mallet scores were collected for each patient. There was 1 case of transient radial nerve palsy with no long-term complications overall.
Ultrafast Transient Absorption Spectroelectrochemistry: Femtosecond to Nanosecond Excited-State Relaxation Dynamics of the Individual Components of an Anthraquinone Redox Couple (2021)

This repository contains the underlying data for the paper entitled "Ultrafast Transient Absorption Spectroelectrochemistry: Femtosecond to Nanosecond Excited-State Relaxation Dynamics of the Individual Components of an Anthraquinone Redox Couple". It includes the input and output files of the implicit solvent TDDFT theoretical calculations performed on singlet and triplet states. Explicit solvent input and output files have also been added, along with the full ESTEEM script; if the ESTEEM script is run with the correct versions of ONETEP, Amber, and NWChem (listed within the corresponding files), the whole workflow of the package used within this work should be reproducible. Moreover, the TEAS data for AQS in pH 4.9 buffer (pump at 330 nm) along with the TEAS data for the reduced species in pH 4.9 buffer (pump at 382 nm) have also been attached; the TEAS results are presented as ΔmOD over a range of wavelengths (320-700 nm) and across different time delays (-0.5 to 2,500 ps). The UV/Vis data for AQS and its reduced species at pH 4.9 and the pH dependence measurements have been additionally included. For more information please consult the main paper.
Phase I study of the investigational drug MLN8237, an Aurora A kinase (AAK) inhibitor, in patients (pts) with solid tumors. (2010)

3010
Adenylosuccinate synthetase: recent developments. (1999)

By exerting strategic control on purine nucleotide biosynthesis, and by engaging GTP-dependent transphosphorylation of IMP to activate loss of an oxygen atom during catalysis, adenylosuccinate synthetase remains as enzyme that justifiably fascinates students of enzyme catalysis. This review describes how the balanced application of X-ray crystallography and enzyme kinetics has advanced the comprehension of the catalytic and regulatory properties of adenylosuccinate synthetase. Detailed analysis has demonstrated the formation of 6-phosphoryl-IMP, an intermediate originally postulated over 40 years ago on the basis of oxygen-18 exchange experiments showing that position-6 oxygen of IMP becomes incorporated into phosphate. Inferences about the participation of amino acid side-chains that stabilize 6-P-IMP during catalysis have also been confirmed by site-directed mutagenesis and examination of such mutations on various kinetic parameters. Moreover, the action of certain regulatory ligands have also been viewed at atomic level resolution. For example, magnesium ion and GDP can induce conformational changes linked to the stabilization of one of two known conformations of the so-called 40s loop. Another significant finding is that two magnesium ions play fundamental roles: one binding with high affinity to the substrate GTP, and a second binding with lower affinity to the co-substrate aspartate. These structural and kinetic studies have also formed the basis for clarifying the action of various inhibitors and potentially important pharmacologic agents with this key regulatory enzyme. Finally, this review explores the current status of investigations on gene structure and gene expression in a number of organisms.
Unsupervised 3D Prostate Segmentation Based on Diffusion-Weighted Imaging MRI Using Active Contour Models with a Shape Prior (2011)

Accurate estimation of the prostate location and volume from in vivo images plays a crucial role in various clinical applications. Recently, magnetic resonance imaging (MRI) is proposed as a promising modality to detect and monitor prostate-related diseases. In this paper, we propose an unsupervised algorithm to segment prostate with 3D apparent diffusion coefficient (ADC) images derived from diffusion-weighted imaging (DWI) MRI without the need of a training dataset, whereas previous methods for this purpose require training datasets. We first apply a coarse segmentation to extract the shape information. Then, the shape prior is incorporated into the active contour model. Finally, morphological operations are applied to refine the segmentation results. We apply our method to an MR dataset obtained from three patients and provide segmentation results obtained by our method and an expert. Our experimental results show that the performance of the proposed method is quite successful.
Structural and spectroscopic studies of rhodium-(I) and -(III) nitrato complexes (1992)

Refluxing Rh(NO3)3·2H2O with an excess of PPh3 in MeOH or EtOH for over 48 h gives trans-[Rh(CO)(ONO2)(PPh3)2]1 whereas the same reaction at room temperature gives predominantly [Rh(ONO2)(PPh3)3]2. X-Ray structure determinations of 1 and 2 show the nitrate groups to be monodentate in each case although disorder of the CO and NO3 groups in 1 precludes accurate determination of bond lengths and angles. There is no reaction of trans-[Rh(CO)X(PPh3)2](X = Cl, F or ONO2) with H2 at room temperature and 1 atm pressure, whereas there is an immediate reaction of [Rh(ONO2)(PPh3)3] under the same conditions to give [RhH2(ONO2)(PPh3)3]3, which has been characterised by multinuclear NMR spectroscopy. Compound 3 loses PPh3 to give [RhH2(O2NO)(PPh3)2]6a which has been characterised similarly and by X-ray crystallography; although the metal-bound hydrogens were not located the structure is clearly based on an octahedral geometry with trans phosphines and a bidentate nitrate.
Turbo channel estimation for OFDM systems on highly time and frequency selective channels (2002)

We propose an optimum turbo channel estimation algorithm for OFDM systems on highly selective fading channels. This algorithm performs an iterative estimation of the multiplicative fading channel according to the maximum a posteriori criterion. It requires a convenient representation of the highly time and frequency selective fading channel based on the Karhunen-Loeve expansion theorem. It also optimally takes into account the coded structure of the data symbols in the estimation process thanks to the Bahl (1974) algorithm.
Nonparametric Bayesian Methods and the Dependent Pitman-Yor Process for Modeling Evolution in Multiple Object Tracking (2019)

In this paper, we propose a family of dependent Pitman-Yor (DPY) processes to model the state prior for multiple object tracking. This process is shown to be more flexible and a better match than the dependent Dirichlet process in tracking a time-varying number of objects. The DPY model directly incorporates learning multiple parameters from correlated information. Integrated with a Dirichlet process mixture model, the overall approach estimates time dependent object cardinality, provides object labeling, and identifies object associated measurements. Using Markov chain Monte Carlo sampling methods, the performance of the DPY based approach is demonstrated and compared to the labeled multi Bernoulli tracker.
La intervención pedagógica en el ámbito de la reflexividad: Un programa educativo para 2º De Primaria (1996)

Elaboramos y aplicamos un programa de intervencion para incrementar la reflexividad de ninos de siete anos, de segundo curso de primaria. Trabajamos este programa en el aula con tecnicas que desarrollamos en ejercicios especificos y tambien incorporamos estas tecnicas en el curriculum ordinario, en Lengua, Matematicas, etc. Obtuvimos excelentes resultados. El incremento de reflexividad fue consistente tres meses despues de concluida la intervencion y produjo mejora en los resultados academicos de los estudiantes. Pensamos que estos resultados son muy importantes porque los estudiantes eran muy jovenes. De hecho, no conocemos ningun otro programa que haya tenido exito en este ambito a tan temprana edad.
Der Einfluß von Steroiden auf die Aktivität von Imidazol‐Antimyzetika (1980)

Zusammenfassung: Die Beeinflußbarkeit der antimyzetischen Aktivität von Imidazol‐Derivaten durch Steroide ist in doppelter Hinsicht interessant. Einerseits bezüglich des Wirkmechanismus der Imidazole, andererseits wegen der möglichen Interaktionen zwischen auf der Hautobdäche normalerweise vorkommenden und therapeutisch aufgetra‐genen Steroiden. Als Beitrag zur Klärung dieser Frage wurde die antimyzetische Aktivität von Clotrima‐zol und Econazolnitrat in Anwesenheit von Cholesterin und Digitonin geprüft. Eine signifhnte Abschwächung der Wirksamkeit in einander gleichen Konzentrationen (Imidazol‐Derivat und Steroid) konnte nicht festgestellt werden. Weitere Untersuchungen beschäftigten sich mit der Einwirkung von N‐148/76 und Ketoconazol auf Candida albicans und auf Staphylococcus aureus haemolyticus in Gegenwart von Triamcinolonacetonid und Prednisolon‐21‐hemisuccinat‐Natrium. Bei Anwesenheit höherer Steroidkonzentrationen ergaben sich signifiante Einfluß‐nahmen: Es trat eine Abschwächung der antimyzetischen Aktivität der Imidazol‐Deri‐vate ein, allerdings nur dann, wenn die genannten Glucocorticoide in höchsten Konzentrationen (bis zum 25 fachen der Imidazolkonzentration) Anwendung fanden. Die anti‐bakterielle Aktivität der Imidazol‐Derivate wird durch Glucocorticoide nicht vermindert. Die klinische Bedeutung der Versuchsergebnisse wird kurz diskutiert. Summary: The influence of steroids on the activity of imidazole derivatives is of double interest: such experiments might contribute to our understanding of the way of action of the antifungal imidazoles and they might inform about interactions between the drugs and steroids normally present on human skin or contained in the respective preparation. Three topical and one systemically active imidazole derivative were investigated (clotrimazol, econazole nitrate, N‐148/76, ketoconazole). The glucocorticoids tested were triamcinolone acetonide and prednisolone‐21‐hemisuccinate‐sodium; further non‐hormonally active steroids assayed were cholesterol and digitonine. The model used was the oxygen consumption of bacteria and yeasts in the resting phase (Warburg assay). Concerning the antibacterial action of the imidazoles, no decrease could be detected in the presence of steroids. However, the antifungal activity was significantly impaired when glucocorticoids in high concentrations (10–25 times higher than the concentrations of the imidazoles) were present in the test flasks.
Phosphoric and electric utility fuel cell technology development. Technical progress report No. 20, for quarter ending December 1984 (1985)

Over 1500 hours and 15 thermal cycles were accumulated on the first 10-ft/sup 2/, 29-cell stack at 120 psia and 450/sup 0/F. Assembly of the second 10-ft/sup 2/ short stack was completed and testing was initiated. Over 11,700 hours and 32 thermal cycles have been accumulated on the 3.7-ft/sup 2/, 30-cell stack at 120 psia and 405/sup 0/F. Catalyst selected for the current 10-ft/sup 2/ short stack performed at the E-line goal for over 5000 hours at 120 psia and 400/sup 0/F in a shop-made subscale cell. This test fulfills the 5000 hour catalyst endurance goal. Two subscale cells cut from 10-ft/sup 2/ electrodes from the same batch as used in the second 10-ft/sup 2/ short stack are performing above E-line after 1000 hours. The cross pressure evaluation of two 3.7-ft/sup 2/, seven cell ..delta..P rigs was completed. Automated equipment to increase processing rates and reduce manpower was used to conduct the substrate treating, catalyst deposition, matrix application and trimming operations of the 10-ft/sup 2/ electrodes for the next short stack. Work was initiated on improved edge seals. Initial trials with new powder transfer equipment indicate a 20 fold increase in powder transfer rate to the substrate forming machine. Themore » fourth mold trial of separator plates was completed. Edge waves in separator plates were reduced with increased restraining loads. 33 figs., 10 tabs.« less
An Investigation into Feature-Level Fusion of Face and Fingerprint Biometrics (2011)

ntroduction The term “biometrics” defines the analysis of unique physiological or behavioral characteristics to verify the claimed identity of an individual. Biometric identification has eventually assumed a much broader relevance as a new technological solution toward more intuitive computer interfaces (Hong et al. 1999; Jain et al. 1999). Multibiometric systems Jain and Ross (2004) have been devised to overcome some of the limitations of unimodal biometric systems. In general terms, the combination of multiple biometric traits is operated by grouping multiple sources of information. These systems utilize more than one physiological or behavioral characteristic, or a combination of both, for enrollment and identification. For example, the problem of nonuniversality can be overcome, because multiple traits together always provide a sufficient population coverage. Multibiometrics also offers an efficient countermeasure to spoofing, because it would be difficult for an impostor to spoof multiple biometric traits of a genuine user simultaneously (Jain and Ross 2004). In some cases the sensor data can be corrupted or noisy, the use of multiple biometric traits always allow to reduce the effects of errors and noise in the data. Ross and Jain (2003) presented a wide overview of multimodal biometrics describing different possible levels of fusion, within several scenarios, modes of operation, integration strategies, and design issues.
Distribution system multi-domain simulation tool for wind power analysis (2016)

This paper develops an integrated multi-domain simulation tool for distribution systems designed specifically for applications in wind power analysis. The proposed tool consists of steady-state, electromechanical transient, and electromagnetic transient models of voltage sources, transmission lines, transformers, capacitor banks, fixed-speed wind turbines (FSWTs), and wide-slip wind turbines (WSWTs). The test circuit used to demonstrate the multi-domain simulation approach is the IEEE four-node test feeder with a wind turbine connected at the feeder end. The simulation is performed for a one-day period. During this period, wind speed changes every ten minutes, a capacitor is switched on and off, and a temporary single-line-to-ground fault occurs. The results show that the proposed multi-domain simulation tool is able to simulate and analyze long-term power system phenomena.
Three-dimensional flows in slowly varying planar geometries (2004)

We consider laminar flow in channels constrained geometrically to remain between two parallel planes; this geometry is typical of microchannels obtained with a single step by current microfabrication techniques. For pressure-driven Stokes flow in this geometry and assuming that the channel dimensions change slowly in the streamwise direction, we show that the velocity component perpendicular to the constraint plane cannot be zero unless the channel has both constant curvature and constant cross-sectional width. This result implies that it is, in principle, possible to design “planar mixers,” i.e., passive mixers for channels that are constrained to lie in a flat layer using only streamwise variations of their in-plane dimensions. Numerical results are presented for the case of a channel with sinusoidally varying width.