gebhart/patrepeval huggingface dataset:
Chondrogenic re-differentiation potential of chondrocytes after monolayer culture: Comparison between osteoarthritis and young adult patients (2019)

Purpose: Monolayer passage of chondrocytes results in dramatic phenotypic changes. This “de-differentiation” is expected to restore the chondrogenic properties such as “re-differentiation” in autologous chondrocyte implantation (ACI). The purpose of this study was to compare the chondrogenic re-differentiation potential of chondrocytes, from osteoarthritis (OA) patients and young adult patients, after monolayer culture. Methods: Chondrocytes from five old patients with knee OA (OAC) and five young patients with recurrent shoulder dislocation (non-OAC) were used. The chondrocytes from passages 1 to 3 were analyzed for the expression of cell surface markers (CD73, CD90, CD105, and CD44) by flow cytometric analysis. Chondrocytes of passage 4 were cultured as pellets for re-differentiation and evaluated histologically. Real-time PCR were performed to measure the chondrogenic related genes transcriptional levels. Results: OAC and non-OAC had comparable positive ratios for CD44, CD73, CD90, and CD105. The expression of CD105 was upregulated from passage 1 to passage 3 in OAC, and it increased at the same level as in non-OAC during passage 2 and 3. The expression of COL2 decreased from passage 1 to passage 3 in both the groups. There were no statistical differences in the Bern Scores between OAC and non-OAC. Conclusion: The chondrocytes from OA patients and young adult patients had chondrogenic re-differentiation potential. The changes in cell surface markers and chondrogenic related genes showed similarity for both the groups. Our findings suggest that OAC can become the cell source for ACI.
Japanese Film and Women : The Works of Mizoguchi Kenjiand Naruse Mikiko ( Cultural and SocialChanges in Respect of Asian Women : Women and Culture) (1996)

Garlic is a polyphenolic and organosulfur enriched nutraceutical spice consumed since ancient times. Garlic and its secondary metabolites have shown excellent health-promoting and disease-preventing effects on many human common diseases, such as cancer, cardiovascular and metabolic disorders, blood pressure, and diabetes, through its antioxidant, anti-inflammatory, and lipid-lowering properties, as demonstrated in several in vitro, in vivo, and clinical studies. The present review aims to provide a comprehensive overview on the consumption of garlic, garlic preparation, garlic extract, and garlic extract-derived bioactive constituents on oxidative stress, inflammation, cancer, cardiovascular and metabolic disorders, skin, bone, and other common diseases. Among the 83 human interventional trials considered, the consumption of garlic has been reported to modulate multiple biomarkers of different diseases; in addition, its combination with drugs or other food matrices has been shown to be safe and to prolong their therapeutic effects. The rapid metabolism and poor bioavailability that have limited the therapeutic use of garlic in the last years are also discussed.
Wedge Dislocation as the Elastic Counterpart of a Crystal Deformation Twin (1968)

A crystal deformation twin may be visualized to form and grow the movement of partial dislocations only if the twinning dislocations especially distributed to give an invariant shear. One consequence of this requirement is that, if a critical resolved twinning stress exists in the same sense twinning as for slip, this stress depends on the reciprocal thickness of the twin. This type of model for twinning may be developed through the use of relatively unknown disclinations, in particular, the wedge dislocation.
Sopravvivenza di Micrococcus spp. e Penicillium spp. isolati da capsule per bottiglie ed inoculati in acque minerali naturali (2003)

E' stata studiata la capacita' di sopravvivenza di un ceppo batterico (Micrococcus spp.) e di uno stipite fungino (Penicillium spp.), isolati dalla superficie di capsule in plastica e inoculati in diversi campioni di acqua minerale naturale. Micrococcus spp. ha mostrato una scarsa capacita' di sopravvivenza, risultando completamente abbattuto sei giorni dopo un inoculo di 9 x 10*[4) ufc/ml in tutti i tipi di acqua minerale utilizzati per lo studio ed entro le 24 ore in acqua addizionata di CO2; Penicillium spp. nelle acque minerali non carbonate e' risultato in grado di sopravvivere a lungo, ma non di moltiplicarsi, mantenendo pressoche' costante la carica durante tutta la sperimentazione (120 giorni), indipendentemente dalla carica iniziale. Al contrario, in acqua minerale addizionata di CO2, dopo una iniziale conservazione della carica, si e' osservato un abbattimento seguito dalla totale scomparsa del ceppo, con tempi differenti in relazione alle cariche iniziali utilizzate
Delivery of services and media: Using keyword-bubble interaction in personal healthcare management (2012)

This paper integrates the interpretation of data into an intuitive HMI addressing the growing demand for personalization in web applications. The resulting K-BMP uses live, interactive bubbles to present information correlated to the intent with which it is used. The behavior of bubbles is controlled through touch or voice commands, and a dual user interface with personalized keywords helps to navigate the user to the target multimedia content. Keyword-bubble combinations can then be uploaded to a central repository from which they may be accessed and cross-referenced with the personalized keywords of others. This essentially converts keywords into a commodity to be traded or manipulated according to individual needs.
Artificial Intelligence–Based Traditional Chinese Medicine Assistive Diagnostic System: Validation Study (2020)

The main contributions of the artificial intelligence-based traditional Chinese medicine assistive diagnostic system proposed in this paper are that 187 commonly known traditional Chinese medicine diseases can be diagnosed and a novel prediction method called an integrated learning model is demonstrated. This new prediction method outperformed all four existing methods in our preliminary experimental results. With further improvement of the algorithms and the availability of additional electronic health record data, it is expected that a wider range of traditional Chinese medicine disease-types could be diagnosed and that better diagnostic accuracies could be achieved.
Volitive Clan PSO - An Approach for Dynamic Optimization Combining Particle Swarm Optimization and Fish School Search (2012)

Volitive Clan PSO - An Approach for Dynamic Optimization Combining Particle Swarm Optimization and Fish School Search
A PC-controlled microwave tomographic scanner for breast imaging (2011)

This article presents the design and development of a personal computer based controller for a microwave tomographic system for breast cancer detection. The system uses motorized, dual-polarized antennas and a custom-made GUI interface to control stepper motors, a wideband vector network analyzer (VNA) and to coordinate data acquisition and archival in a local MDSPlus database. Both copolar and cross-polar scattered field components can be measured directly. Experimental results are presented to validate the various functionalities of the scanner.
Mouse molecular genetics (2010)

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology. The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text: o Lentivirus transgenesis o Germline modification using mouse stem cells o Electroporation o Applications of transposons in mouse genetics o Functional genomics using transposon systems o The use of DNA transposons in detecting cancer genes in mice o Recombination, conditional mutagenesis and induction of tamoxifen o Genetic fate mapping using recombinases o Genetic screens mouse ES cells o Gene trap mutagenesis o Mouse mutagenesis o Self- renewal and pluripotency o Transgenic RNAi applications o Gene knockdowns o Tetracycline-controlled transcription o Gene expression profiling of mouse embryos The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. It comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.
Computer-based Physician Order Entry: The State of the Art (1994)

Direct computer-based physician order entry has been the subject of debate for over 20 years. Many sites have implemented systems successfully. Others have failed outright or flirted with disaster, incurring substantial delays, cost overruns, and threatened work actions. The rationale for physician order entry includes process improvement, support of cost-conscious decision making, clinical decision support, and optimization of physicians' time. Barriers to physician order entry result from the changes required in practice patterns, roles within the care team, teaching patterns, and institutional policies. Key ingredients for successful implementation include: the system must be fast and easy to use, the user interface must behave consistently in all situations, the institution must have broad and committed involvement and direction by clinicians prior to implementation, the top leadership of the organization must be committed to the project, and a group of problem solvers and users must meet regularly to work out procedural issues. This article reviews the peer-reviewed scientific literature to present the current state of the art of computer-based physician order entry.
Non-Cooperative Power Control for Faded Wireless Ad Hoc Networks (2007)

The problem of non-cooperative power control is studied for wireless ad hoc networks supporting data services. We develop a maximum throughput based non-cooperative power control game (MT-NPG), where each transmitter node requires only the <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">effective</i> <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">interference</i> measurement from its corresponding receiver. An expression for the optimal power control to maximize the average transmission rate for each individual link is derived. Then, we design a distributed iterative power updating algorithm to approximate the optimal solution. The existence and uniqueness of Nash equilibrium for the proposed game is investigated. Simulation results show that the proposed MT-NPG yields a significant improvement in the sum of average achievable rates when compared with existing approaches.
Serum leptin levels in children and adolescents with insulin-dependent diabetes mellitus in relation to metabolic control and body mass index (1998)

The ob protein, termed leptin, is produced by adipocytes and is thought to act as an afferent satiety signal regulating weight through suppressing appetite and stimulating energy expenditure in humans and/or rodents. Insulin has been found to be a potent stimulator of leptin expression in rodents. It is unclear at present whether this insulin action is a direct or an indirect effect. To investigate whether leptin concentrations in children and adolescents with type 1 diabetes (IDDM) were related to metabolic status, body weight, body mass index and insulin treatment, we have measured leptin concentrations in serum from 13 newly diagnosed IDDM patients before the beginning of insulin treatment (8 girls, 5 boys, aged 4.7-17.5 years) and in 134 patients with IDDM during treatment (64 girls, 70 boys, aged 2.6-20.1 years) using a specific radioimmunoassay. The data from patients with diabetes were compared with normative data that were derived from a large cohort of healthy children and adolescents. Serum from children with newly diagnosed diabetes had significantly lower levels of leptin (mean 1.28+/-1.60 ng/ml, range 0.14-6.13 ng/ml) compared with healthy children (n=710) (mean 2.2 ng/ml, range 0.26-14.4ng/ml) and compared with insulin-treated children and adolescents (mean 5.18+/-5.48 ng/ml, range 0.26-29.77 ng/ml) (P<0.0001) even after adjustment for gender and body mass index (BMI). Serum leptin levels in patients with IDDM were significantly correlated with BMI (r=0.42, P<0.0001). Multiple regression analysis showed that age and BMI were significantly correlated with leptin levels, while duration of diabetes, mean HbA1c levels, insulin dose and plasma glucose, triglyceride and cholesterol levels were not. Females had higher serum leptin concentrations than males even when adjusted for BMI (P<0.0001). Surprisingly and most importantly, leptin levels in insulin-treated young adult (Tanner stage 5) patients were significantly higher than values found in the healthy nondiabetic reference population when adjusted for sex, Tanner stage and BMI. These findings suggest that leptin levels in IDDM patients show a similar dependency on adipose tissue and age as in healthy, normal children. The data provide evidence that insulin may be of importance as a regulator of serum leptin levels in vivo not only in rodents but also in humans. It is hypothesized that the elevated BMI-adjusted leptin levels in adolescents with IDDM could indicate either that these patients may be oversubstituted by the intensified insulin therapy that they are receiving or that their body composition and body fat content may differ from that of healthy adolescents in the sense that they have a relative increase in fat mass.
Succinate prodrugs in combination with atropine and pralidoxime protect cerebral mitochondrial function in a rodent model of acute organophosphate poisoning (2022)

Pesticides account for hundreds of millions of cases of acute poisoning worldwide each year, with organophosphates (OPs) being responsible for the majority of all pesticide-related deaths. OPs inhibit the enzyme acetylcholinesterase (AChE), which leads to impairment of the central- and peripheral nervous system. Current standard of care (SOC) alleviates acute neurologic-, cardiovascular- and respiratory symptoms and reduces short term mortality. However, survivors often demonstrate significant neurologic sequelae. This highlights the critical need for further development of adjunctive therapies with novel targets. While the inhibition of AChE is thought to be the main mechanism of injury, mitochondrial dysfunction and resulting metabolic crisis may contribute to the overall toxicity of these agents. We hypothesized that the mitochondrially targeted succinate prodrug NV354 would support mitochondrial function and reduce brain injury during acute intoxication with the OP diisopropylfluorophosphate (DFP). To this end, we developed a rat model of acute DFP intoxication and evaluated the efficacy of NV354 as adjunctive therapy to SOC treatment with atropine and pralidoxime. We demonstrate that NV354, in combination with atropine and pralidoxime therapy, significantly improved cerebral mitochondrial complex IV-linked respiration and reduced signs of brain injury in a rodent model of acute DFP exposure.
A Recurrent Encoder-Decoder Network for Sequential Face Alignment (2016)

We propose a novel recurrent encoder-decoder network model for real-time video-based face alignment. Our proposed model predicts 2D facial point maps regularized by a regression loss, while uniquely exploiting recurrent learning at both spatial and temporal dimensions. At the spatial level, we add a feedback loop connection between the combined output response map and the input, in order to enable iterative coarse-to-fine face alignment using a single network model. At the temporal level, we first decouple the features in the bottleneck of the network into temporal-variant factors, such as pose and expression, and temporal-invariant factors, such as identity information. Temporal recurrent learning is then applied to the decoupled temporal-variant features, yielding better generalization and significantly more accurate results at test time. We perform a comprehensive experimental analysis, showing the importance of each component of our proposed model, as well as superior results over the state-of-the-art in standard datasets.
Biomimetic optical filter based chemical sensing techniques (2014)

Biomimetic, filter-based chemical detectors have been designed to detect various chemicals of interest. We present some of the existing approaches, and a new comparative-discrimination spectral detection approach for the detection of overlapping chemical spectral signatures.
An Optimal Pricing Scheme for the Energy-Efficient Mobile Edge Computation Offloading With OFDMA (2018)

The mobile edge computation offloading (MECO) system has emerged as a promising technology to address several problematic issues in cloud computing such as data throughput, capacity, and security. In this letter, we consider a single-cloud-multi-user MECO system with orthogonal frequency-division multiple access and propose an optimal pricing scheme to consider each mobile user's (MU) need for resources. The proposed model was formulated as a single-leader-multi-user Stackelberg game model, and the Stackelberg equilibrium was provided to optimize the utility of the MUs and the edge cloud. Here, the utility of the MU implies the energy efficiency and payment for using the computation capacity of the edge cloud, and the utility of the edge cloud implies the revenue for serving computation offloading. The optimal strategies of the MUs are uniquely given by closed-form expressions, and that of the edge cloud is given by gradient descent methods. Some numerical examples are provided to verify our approach.
Confronting Genetic Testing Disparities (2005)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Rate of Somatic Mutation in Immunoglobulin Production by Mouse Myeloma Cells (1971)

Cultures of mouse myeloma cells which secrete both heavy (H) and light (L) immunoglobulin chains were cloned in soft agar. Variants that synthesized neither chain, or only light chains, were detected by overlaying the growing colonies with antiserum specific for the individual immunoglobulin chains. The rate of conversion of heavy plus light chain producers to light chain producers was 1.1 x 10(-3) per cell per generation, as determined by fluctuation analysis.
Role of early‐ or late‐phase activation of p38 mitogen‐activated protein kinase induced by tumour necrosis factor‐α or 2,4‐dinitrochlorobenzene during maturation of murine dendritic cells (2003)

Dendritic cells (DCs) are maturated by a variety of stimuli. However, the precise mechanisms underlying the maturation of DCs are not fully understood. In the present study, we analysed the effects of tumour necrosis factor-alpha (TNF-alpha) and 2,4-dinitrochlorobenzene (DNCB) on phenotypic maturation and p38 mitogen activated protein kinase (MAPK) activity, using a murine DC line. TNF-alpha markedly increased the surface expression of major histocompatibility complex (MHC) and costimulatory molecules, CD86 and CD80, on DCs. DNCB more markedly enhanced the surface expression of costimulatory molecules, but showed less stimulatory capability on MHC molecules, compared with TNF-alpha. Simultaneous treatment of DCs with TNF-alpha and DNCB showed additive enhancement of costimulatory molecule expression. TNF-alpha activated p38 MAPK in DCs only at an early time-point (15 min). In contrast, DNCB activated p38 MAPK at later time-points (3-6 hr). SB203580, a specific inhibitor of p38 MAPK, partially or markedly inhibited the phenotypic changes of DCs induced by TNF-alpha or DNCB, respectively. In addition, N-acetyl-l-cysteine, a reducing supplier, completely inhibited the DNCB-induced expression of MHC and costimulatory molecules, but not those induced by TNF-alpha. These findings demonstrate that TNF-alpha and DNCB activate the p38 MAPK pathway at an early and a late phase, respectively, and thereby induce DC maturation through different signal pathways.
Assessment of physiological state of microorganisms in activated sludge with flow cytometry: application for monitoring sludge production minimization (2008)

Many sludge reduction processes have been studied for the minimization of sludge production in biological wastewater treatment. The investigations on most of these processes have monitored the increase of the soluble chemical oxygen demand, the sludge mass reduction, or the decrease of the floc size, but little information has been obtained on cell lysis and the change of the biological cell activity. However, employing any strategy for reducing sludge production may have an impact of microbial community in biological wastewater treatment process. This impact may influence the sludge characteristics and the quality of effluent. The objective of this study concerns the determination of the physiological state of activated sludge microorganisms during a sludge minimization process. A thermal treatment at 80 degrees C for 5, 20, 40 and 60 min was chosen in this study. Staining bacteria with CTC and SYTOX green was used to evaluate biological cell activity and viability of cell types contained in activated sludge, respectively. The monitoring of cell activity and viability was performed using flow cytometry (FCM) analysis before and after thermal treatment of activated sludge. Results indicated an increase in the number of permeabilized cells and a decrease in the number of active cells, subsequent to the thermal treatment. The study also confirms the potential of FCM to successfully evaluate the physiological heterogeneity of an activated sludge bacterial population. Moreover, the experimentally observed correlations between the FCM results and the organic matter solubilization in activated sludge samples during thermal treatment revealed that the increase in the soluble organic matter concentration was predominantly due to an intracellular material release. Identifying the increase in activated sludge hydrolysis requires a precise knowledge of the involved mechanisms, and this study indicated that the FCM, used in conjunction with specific probes, could be a useful tool.