gebhart/patrepeval huggingface dataset:
In Vitro Diagnostic Assay to Detect SARS-CoV-2-Neutralizing Antibody in Patient Sera Using Engineered ACE-2 Mini-Protein (2022)

The recent development and mass administration of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) vaccines allowed for disease control, reducing hospitalizations and mortality. Most of these vaccines target the SARS-CoV-2 Spike (S) protein antigens, culminating with the production of neutralizing antibodies (NAbs) that disrupt the attachment of the virus to ACE2 receptors on the host cells. However, several studies demonstrated that the NAbs typically rise within a few weeks after vaccination but quickly reduce months later. Thus, multiple booster administration is recommended, leading to vaccination hesitancy in many populations. Detecting serum anti-SARS-CoV-2 NAbs can instruct patients and healthcare providers on correct booster strategies. Several in vitro diagnostics kits are available; however, their high cost impairs the mass NAbs diagnostic testing. Recently, we engineered an ACE2 mimetic that interacts with the Receptor Binding Domain (RBD) of the SARS-2 S protein. Here we present the use of this engineered mini-protein (p-deface2 mut) to develop a detection assay to measure NAbs in patient sera using a competitive ELISA assay. Serum samples from twenty-one patients were tested. Nine samples (42.8%) tested positive, and twelve (57.1%) tested negative for neutralizing sera. The data correlated with the result from the standard commercial assay that uses human ACE2 protein. This confirmed that p-deface2 mut could replace human ACE2 in ELISA assays. Using bacterially expressed p-deface2 mut protein is cost-effective and may allow mass SARS-CoV-2 NAbs detection, especially in low-income countries where economical diagnostic testing is crucial. Such information will help providers decide when a booster is required, reducing risks of reinfection and preventing the administration before it is medically necessary.
Neutralizing antibodies to multiple sclerosis treatments. (2004)

In light of these data, the immunogenicity of IFNbeta products should be considered prior to initiating treatment with IFNbeta. Also, ongoing laboratory monitoring of patients treated with higher-dose IFNbeta is recommended for early detection of NAbs.
Vision-Based Detection of Unusual Patient Activity (2011)

Automated patient monitoring in hospital environments has gained increased attention in the last decade. An important problem is that of behaviour analysis of psychiatric patients, where adequate monitoring can minimise the risk of harm to hospital staff, property and to the patients themselves. For this task, we perform a preliminary investigation on visual-based patient monitoring using surveillance cameras. The proposed method uses statistics of optical flow vectors extracted from the patient movements to identify dangerous behaviour. In addition, the method also performs foreground segmentation followed by blob tracking in order to extract shape and temporal characteristics of blobs. Dangerous behaviour includes attempting to break out of safe-rooms, self-harm and fighting. The features considered include a temporal and multi-resolution analysis of blob coarseness, blob area, movement speed and position in the room. This information can also be used to normalise the other features according to estimated position of the patient in the room. In this preliminary study, experiments in a real hospital scenario illustrate the potential applicability of the method.
Vaccine-induced protection against 3 systemic mycoses endemic to North America requires Th17 cells in mice (2016)

The authors recently became aware that the IL-1R mice used for the original Supplemental Figure The experiment with IL1r -/-animals was repeated, and the correct Supplemental Figure The correct text describing the experiments in the Results and Discussion sections appears below.
Genetic evolution of pancreatic cancer: lessons learnt from the pancreatic cancer genome sequencing project (2011)

Pancreatic cancer is a disease caused by the accumulation of genetic alterations in specific genes. Elucidation of the human genome sequence, in conjunction with technical advances in the ability to perform whole exome sequencing, have provided new insight into the mutational spectra characteristic of this lethal tumour type. Most recently, exomic sequencing has been used to clarify the clonal evolution of pancreatic cancer as well as provide time estimates of pancreatic carcinogenesis, indicating that a long window of opportunity may exist for early detection of this disease while in the curative stage. Moving forward, these mutational analyses indicate potential targets for personalised diagnostic and therapeutic intervention as well as the optimal timing for intervention based on the natural history of pancreatic carcinogenesis and progression.
Compressible pairwise interaction extended point-particle model for force prediction of shock-particle bed interaction (2023)

Euler-Lagrange simulations are often required to study complex compressible multiphase problems. Such systems include volcanic explosions and supernovas. We developed an unsteady neighboring particle interaction framework with the help of the compressible Maxey-Riley-Gatignol equation and the scattering theory, which further improves force predictions used in Euler-Lagrange simulations.
Heterozygote Punktmutationen in der p150 Untereinheit des Dynactin-Gens bei der amyotrophen Lateralsklerose (2004)

Hintergrund: Der molekulare Motor Dynein sowie der assoziierte Multiprotein-Komplex Dynactin sind am retrograden axonalen Transport von Organellen und Vesikeln beteiligt. Eine Funktionsstörung des Dyneins und Dynactins gilt als ein pathogenetischer Aspekt der selektiven Motoneurondegeneration. In diesem Zusammenhang wurde von uns erstmalig bei 250 Patienten mit amyotropher Lateralsklerose (ALS) sowie 150 Kontrollen gezielt nach Sequenzveränderungen in der p150 Untereinheit des Dynein-Gens (DCTN1) und in der schweren Kette des Dynactin-Gens (DNCHC1) gesucht.
Prevalence of enteroaggregative Escherichia coli and its virulence-related genes in a case–control study among children from north-eastern Brazil (2013)

Enteroaggregative Escherichia coli (EAEC) is an important agent that causes endemic and epidemic diarrhoeal diseases worldwide. Several EAEC virulence-related genes (VRGs) have been described but their role in the clinical outcome of infection is not completely defined. This study investigated the prevalence of EAEC and potential associations of its VRGs with risk of or protection from diarrhoeal diseases in children from urban communities in north-eastern Brazil. The case-control study included 166 children, who had their stools evaluated for the EAEC diagnostic genes (aaiC and aatA) using PCR. Positive samples were further analysed by multiplex PCR and identified 18 VRGs. EAEC was found in the same proportion in both groups (41%). The plasmid-borne gene encoding a hexosyltransferase homologue (capU) was the most frequently detected (89.6%), followed by dispersin protein (aap, 58.2%) and EAEC HilA homologue (eilA, 57.8%). The AAF/III fimbrial subunit (agg3A) gene was observed at lower frequency (1.5%). Plasmid-encoded toxin (pet) or AAF/II fimbrial subunit (aafA) was associated significantly with disease. AAF/IV fimbrial subunit (agg4A) or hypothetical plasmid-encoded haemolysin (orf61) was detected significantly more in controls than in children with diarrhoea. In addition, one set of genes in combination, aaiC and agg3/4C but lacking agg4A and orf61, was associated with diarrhoea cases; and another one, orf61 in the absence of pet and aafA, was correlated with control children. These data confirm a high prevalence, endemicity and heterogeneity of EAEC strains in the developing urban areas of north-eastern Brazil. Statistical correlation between cases and controls was seen with either isolated or combined sets of genes, suggesting that the pathophysiology of EAEC infection involves a complex and dynamic modulation of several VRGs.
Advanced metal and dielectric barrier cap films for Cu low k interconnects (2014)

Multi-layer SiN barrier film with high breakdown and low leakage is developed for Cu low k interconnects and is compared with the SiCNH barrier film used at previous technology nodes. Ultra-thin SiN barrier cap film also provides high conformality and fills recess in Cu lines observed post CMP. A significant enhancement in electro migration (EM) performance was obtained by selectively depositing Co on top of Cu lines followed by conformal multi-layer SiN barrier film. Further EM lifetime improvement is obtained by using a Co liner to form a wrap around structure with completely encapsulated Cu. An integrated in-situ preclean/ metal/dielectric cap chamber was used to avoid any oxidation of Cu/Co layers. Kinetic studies of CVD Co liner/Co cap samples show significant increase in EM activation energy (1.7 eV) over samples with dielectric only barrier film (0.9-1 eV). The complete wrap around structure with Co liner and Co cap shows improved device reliability.
Electrocatalytic behavior of freely-diffusing and immobilized synthetic flavins in aqueous media (2016)

The electrocatalytic activity of three synthetic flavins toward the oxygen reduction reaction was compared to establish structure–property relationships.
Mixture of Ivy Leaves and Coptidis rhizoma Suppresses Pulmonary Inflammation Induced by Air Particles- and Lipopolysaccharide-induced Chronic Obstructive Pulmonary Disease in Mice (2019)

Chronic obstructive pulmonary disease (COPD) is characterized by an elevated number of immune cells and inflammatory cytokines leading to inflammation of the lungs. Air particles including fine dust and sand dust cause respiratory problems and severe inflammation along with lipopolysaccharide (LPS) to induce COPD. HHCR consists of hederacoside C of Ivy leaf dry extract (HH) and berberine of Coptidis rhizoma dry extract (CR). Also, HHCR is a herbal product approved in Republic of Korea for the relief of cough and sputum formation due to acute respiratory infection and inflammation. Although the mechanism of action of hederacoside C and berberine are known, details of the therapeutic effect of their mixture (HHCR) in COPD are not known. Therefore, we aimed to clarify the effect of HHCR on LPSinduced COPD in a mouse model co-exposed to air particles (LPS/air particle-induced COPD mouse model). We used western blotting, ELISA and PCR analyses to quantify the inflammatory proteins that were involved in these effects. Air particles caused significant inflammation in the airway and pathological changes in the lung tissue in mice by activating of NF-κB signaling proteins such as IκB and p65. In contrast, HHCR significantly decreased the infiltration of immune cells into the lung and NF-κB signaling proteins, thereby suppressing inflammation. These data suggest that HHCR has anti-inflammatory effect on COPD mouse model through inactivation of NF-κB signaling.
The pH-Responsive PacC Transcription Factor of Aspergillus fumigatus Governs Epithelial Entry and Tissue Invasion during Pulmonary Aspergillosis (2014)

Destruction of the pulmonary epithelium is a major feature of lung diseases caused by the mould pathogen Aspergillus fumigatus. Although it is widely postulated that tissue invasion is governed by fungal proteases, A. fumigatus mutants lacking individual or multiple enzymes remain fully invasive, suggesting a concomitant requirement for other pathogenic activities during host invasion. In this study we discovered, and exploited, a novel, tissue non-invasive, phenotype in A. fumigatus mutants lacking the pH-responsive transcription factor PacC. Our study revealed a novel mode of epithelial entry, occurring in a cell wall-dependent manner prior to protease production, and via the Dectin-1 β-glucan receptor. ΔpacC mutants are defective in both contact-mediated epithelial entry and protease expression, and significantly attenuated for pathogenicity in leukopenic mice. We combined murine infection modelling, in vivo transcriptomics, and in vitro infections of human alveolar epithelia, to delineate two major, and sequentially acting, PacC-dependent processes impacting epithelial integrity in vitro and tissue invasion in the whole animal. We demonstrate that A. fumigatus spores and germlings are internalised by epithelial cells in a contact-, actin-, cell wall- and Dectin-1 dependent manner and ΔpacC mutants, which aberrantly remodel the cell wall during germinative growth, are unable to gain entry into epithelial cells, both in vitro and in vivo. We further show that PacC acts as a global transcriptional regulator of secreted molecules during growth in the leukopenic mammalian lung, and profile the full cohort of secreted gene products expressed during invasive infection. Our study reveals a combinatorial mode of tissue entry dependent upon sequential, and mechanistically distinct, perturbations of the pulmonary epithelium and demonstrates, for the first time a protective role for Dectin-1 blockade in epithelial defences. Infecting ΔpacC mutants are hypersensitive to cell wall-active antifungal agents highlighting the value of PacC signalling as a target for antifungal therapy.
Photoelectrochemical etching of semiconductors (1998)

Photoelectrochemical (PEC) etching of III–V semiconductors has been used to fabricate unique structures in electronic and photonic devices, such as integral lenses on light-emitting diodes, gratings on laser structures, and through-wafer via connections in field-effect transistors. The advantages and characteristics of PEC etching are reviewed, and the extension of this processing technique to silicon is addressed. Three-dimensional structures are of great interest in silicon for electronic and micromechanical devices. Silicon is a challenging material to PEC-etch because the oxides formed during etching inhibit the dissolution rate and decrease the spatial resolution. In addition, the long carrier lifetime permits holes to react at unilluminated sites. Nonaqueous solvents provide a processing environment where oxides do not interfere with the spatial resolution and free fluoride is no longer needed in the dissolution of silicon.
A mutation in the human apolipoprotein A-I gene. Dominant effect on the level and characteristics of plasma high density lipoproteins (1991)

Epidemiologic and genetic data suggest an inverse relationship between plasma high density lipoprotein (HDL) cholesterol and the incidence of premature coronary artery disease. Some of the defects leading to low levels of HDL may be a consequence of mutations in the genes coding for HDL apolipoproteins A-I and A-II or for enzymes that modify these particles. A proband with plasma apoA-I and HDL cholesterol that are below 15% of normal levels and with marked bilateral arcus senilis was shown to be heterozygous for a 45-base pair deletion in exon four of the apoA-I gene. This most likely represents a de novo mutation since neither parent carries the mutant allele. The protein product of this allele is predicted to be missing 15 (Glu146-Arg160) of the 22 amino acids comprising the third amphipathic helical domain. The HDL of the proband and his family were studied. Using anti-A-I and anti-A-II immunosorbents we found three populations of HDL particles in the proband. One contained both apoA-I and A-II, Lp(A-I w A-II); one contained apoA-I but no A-II, Lp(A-I w/o A-II); and the third (an unusual one) contained apoA-II but no A-I. Only Lp(A-I w A-II) and (A-I w/o A-II) were present in the plasma of the proband's parents and brother. Analysis of the HDL particles of the proband by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed two protein bands with a molecular mass differing by 6% in the vicinity of 28 kDa whereas the HDL particles of the family members exhibited only a single apoA-I band. The largely dominant effect of this mutant allele (designated apoA-ISeattle) on HDL levels suggests that HDL particles containing any number of mutant apoA-I polypeptides are catabolized rapidly.
Multi-FPGA implementation of a Network-on-Chip based many-core architecture with fast barrier synchronization mechanism (2010)

In this paper, we propose a fast barrier synchronization mechanism, targeting Network-on-Chip based many-core architectures. Its salient feature is that, once the barrier condition is reached, the “barrier release” acknowledgement is routed to all processor nodes in a broadcast way in order to save area by avoiding storing source node information and to minimize completion time by eliminating serialization of barrier releasing. Then, we construct a multi-FPGA platform using Xilinx <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">®</sup> Virtex 5 as FPGA chips and implement a NoC based many-core architecture on it. FPGA utilization and simulation results show that our mechanism demonstrates both area and performance advantages over the barrier synchronization counterpart with unicast barrier releasing.
A comparison of tilt current meters and an acoustic doppler current meter in vineyard sound, Massachusetts (2015)

The relatively high cost of acoustic current meters has placed practical limits on the use of these meters in marine research projects. One approach to reducing the cost of current measurements has been the re-invention of the Tilt Current Meter (TCM). Recent developments in accelerometers, magnetometers and low-power non-volatile memory have made it possible to make small, accurate and inexpensive TCMs. Here we describe the design and calibration of the Lowell Instruments TCM and validate its performance in Vineyard Sound, Massachusetts. The performance of four TCMs was compared to an acoustic Doppler current profiler reference meter. The TCMs exhibited an R squared correlation of 0.97-0.98 as well as low slope and offset errors. Based on this demonstrated accuracy we believe the TCM would be useful for a variety of research projects especially where there is a risk of instrument loss or where increased spatial density of measurements is desired.
MEXICANA Y UNIVERSAL ES NUESTRA REVOLUCION (1963)

EN LOS CURSOS DE INVIERNO DE ESCUELA NACIONAL DE CIENCIAS POLITICAS Y SOCIALES, JESUS SILVA HERZOG DIJO QUE LA REVOLUCION FUE UN MOVIMIENTO SOCIAL Y REFUTO TESIS DE ORIGINALIDAD ORIGINALISIMA DE ESTE MOVIMIENTO PUES AUNQUE FUE MEXICANO A VEZ ES UNIVERSAL. TAMBIEN HIZO REFERENCIA A CONSTITUCION DE 1917 DONDE HAY ARTICULOS DE CORTE LIBERAL, POR EJEMPLO EL ARTICULO 27, EL CUAL SENALA QUE HAY QUE ACEPTAR EXPROPIACION DE RIQUEZA A CAUSA DE UTILIDAD PUBLICA COMO DERECHO NACIONAL.
Interleukin-1 beta regulates human cytotrophoblast metalloproteinase activity and invasion in vitro. (1994)

During early human pregnancy, fetal cytotrophoblasts rapidly invade the uterus. This process has many similarities to tumor invasion, except that the extent and the timing of cytotrophoblast invasion are carefully regulated. Therefore, this system is particularly useful for studying mechanisms that regulate invasive processes. Previously, we showed that production and activation of the 92-kDa type IV collagenase (matrix metalloproteinase(MMP)-9) is necessary for cytotrophoblast invasion in vitro. In other systems, interleukin (IL)-1 beta is an important regulator of matrix-degrading metalloproteinases. Therefore, we investigated trophoblast production of IL-1 beta and its receptors, as well as the effects of this cytokine on cytotrophoblast metalloproteinase activity and invasion. The results showed that release of IL-1 beta parallels the invasive potential of the cytotrophoblasts; the highest levels are produced by first trimester cells and the lowest levels by term cells. Immunoprecipitation showed that cytotrophoblasts express the 80-kDa type I IL-1 receptor, suggesting that autocrine effects are possible. IL-1 beta stimulated trophoblast MMP-9 secretion (by a mechanism that required nascent mRNA and protein synthesis) as well as metalloproteinase activity and invasion of Matrigel. Increasing (by lipopolysaccharide treatment) or decreasing (by glucocorticoid treatment) IL-1 beta production had parallel effects on MMP-9 secretion, metalloproteinase activity, and invasion. Because IL-1 beta and corticosteroids are present in high concentrations at the maternal-fetal interface, normal trophoblast invasion may be regulated, in part, by their opposing actions. In contrast, stimulation of cytotrophoblast IL-1 beta secretion by lipopolysaccharide may play a role in the sequela of infected fetal membranes.
Att handla med hjärta eller hjärna – En empirisk studie av konsumenters värderingar vid livsmedelsinköp online (2018)

Titel: Att handla med hjarta eller hjarna – En empirisk studie av konsumenters varderingar vid
livsmedelsinkop 

Seminariedatum: 2018-01-12

Kurs: FEKH29 Foretagsekonomi: Examensarbete i marknadsforing pa kandidatniva (15 hogskolepoang)

Forfattare: Hedvig Franzen-Brunius, Mattias Palm & Daniel Thorner Handledare: Clara Gustafsson

Nyckelord: Customer experience, online customer experience, aterkopsintention, hedonistiska variabler och utilitaristiska variabler

Syfte: Syftet med studien ar att utifran hedonistiska- samt utilitaristiska nyttor undersoka vad konsumenter varderar nar de handlar livsmedel online. Sedemera ar intentionen att identifiera de variabler som har storst paverkan pa customer experience vid livsmedelsinkop online, och darigenom erhalla kunskap om vilka aspekter som genererar aterkommande kunder.

Metod: Studien ar kvantitativ och anvander en abduktiv forskningsmetod for att med bakgrund i relevant teori utveckla och testa hypoteser for att sedan dra logiska slutsater. Inledningsvis arbetade vi induktivt for att utveckla och modifiera en konceptuell modell sa att den battre skulle omfatta domanen for livsmedelsbutiker online. Darefter arbetade vi deduktivt for att bekrafta eller forkasta hypoteser gallande modellen. For insamling av primardata distribuerades en webbenkat och for insamling av sekundardata nyttjades tidigare forskning kring customer experience. Alla insamlade data har genomgatt en kritisk granskning.

Teoretiska perspektiv: Studien ar baserad pa teorier och tidigare forskning om online customer experience (OCE), med utgangspunkt i en konceptuell OCE-modell som presenterats av Rose et al. (2012). Vidare diskuteras hedonistiska och utilitaristiska variablers tankbara betydelse vid livsmedelsinkop online utifran ett resonemang om det kognitiva beslutsfattandet (Kahneman, 2011) och produkters olika grad av engagemang (Dahlen & Lange, 2009).

Empiri: Den empiriska datan baseras pa en strukturerad webbenkat som har undersokt konsumenters attityd i forhallande till en uppsattning aspekter som ar associerade med att handla livsmedel online. Vidare innehaller empirin deskriptiv data i form av demografiska data och hur ofta som konsumenter handlar livsmedel online.

Slutsats: Vi fann att bekvamlighetsaspekten med att handla livsmedel online ar det viktigaste for att konsumenter ska kunna tanka sig att gora aterkop. Vidare ar det storsta hotet for att konsumenter ska gora sina livsmedelsinkop online att de inte kan klamma eller kanna pa sina varor fore inkop, i kombination med att sensoriska intryck ar begransade online. Anvandarvanliga hemsidor, en hog grad av kundanpassning och stora produktsortiment ska ocksa betraktas som hygienfaktorer vid livsmedelsinkop online. Slutligen visade det sig att de utilitaristiska aspekterna har storst betydelse for att konsumenter ska handla livsmedel online. Det kan daremot argumenteras for att enkatens utformning har snedvridit betydelsen av utilitaristiska varden.