gebhart/patrepeval huggingface dataset:
Chicken-derived RSPO1 and WNT3 contribute to maintaining longevity of chicken intestinal organoid cultures (2022)

Intestinal organoids are advanced cellular models, which are widely used in mammalian studies to mimic and study in vivo intestinal function and host-pathogen interactions. Growth factors WNT3 and RSPO1 are crucial for the growth of intestinal organoids. Chicken intestinal organoids are currently cultured with mammalian Wnt3a and Rspo1, however, maintaining their longevity has shown to be challenging. Based on the limited homology between mammalian and avian RSPO1, we expect that chicken-derived factors are required for the organoid cultures. Isolated crypts from embryonic tissue of laying hens were growing in the presence of chicken WNT3 and RSPO1, whereas growth in the presence of mammalian Wnt3a and Rspo1 was limited. Moreover, the growth was increased by using Prostaglandin E2 (PGE<sub>2</sub>) and a Forkhead box O1-inhibitor (FOXO1-inhibitor), allowing to culture these organoids for 15 passages. Furthermore, stem cells maintained their ability to differentiate into goblets, enterocytes and enteroendocrine cells in 2D structures. Overall, we show that chicken intestinal organoids can be cultured for multiple passages using chicken-derived WNT3 and RSPO1, PGE<sub>2</sub>, and FOXO1-inhibitor.
Long - term Storage and Cannabis Oil Stability (2012)

IRENNE GABRIELA TROFIN1*, GABRIEL DABIJA1, DANUT–IONEL VAIREANU2, LAURENTIU FILIPESCU3 1General Inspectorate of Romanian Police, Central Laboratory for Drug Analysis and Profiling, Bucharest, Romania. 2University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Applied Physical Chemistry and Electrochemistry, 313 Splaiul Independentei, 060042, Bucharest, Romania 3University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department Technology of Inorganic Substances and Environmental Protection, 313 Splaiul Independentei, 060042, Bucharest, Romania
Wave Energy Research at Uppsala University and The Lysekil Research Site, Sweden : A Status Update (2015)

This paper provides a summarized status update ofthe Lysekil wave power project. The Lysekil project is coordinatedby the Div. of Electricity, Uppsala University since 2002, with theobjective to de ...
Vibrational Density of States of Thin Films Measured Scattering of Synchrotrons Radiation (1998)

Tbe submitted manuscript has been created by the University of Chicago as Operator of Argonne Na!ional Laboratory (“Argoorre”) under Cantract No. W-31-109-ENG-38 with the U.S. Department of Energy. The U.S. Government retains for itsclfj and others acdng on its behalfj a paid-up, nonexclusive, irrevocable wnrldwide Iiccaac in said article to reproducq prepare derivative wok distribute copies to the public, and pcrfomo publicly aod disp[ay publicly, by or on behatf of the Government.
Unifying Input-Output and State-Space Perspectives of Predictive Control (1998)

Predictive control refers to the concept where the current control decision is based on a prediction of the system controlled response a number of times into the future. Originally derived from input-output models, recent years have seen efforts to interpret and develop predictive control from the state-space domain. This paper presents a formulation that unifies the two perspectives. Although a general state-space model is used as the starting point of the derivation, the predictive controller gains are ultimately synthesized from input-output data. There is no need for explicit model identification, and no observer is required in the implementation. Central to this development is a special interaction matrix which can be explained in the context of a “generalized” Cayley-Hamilton theorem. Experimental results are provided to illustrate this unified perspective of predictive control, including a comparison between data-based and modelbased predictive controller designs.
On the role of representation in the analysis of visual spacetime (2010)

The problems under consideration in this dissertation centre around the representation of visual spacetime, i.e., (visual) image intensity (irradiance) as a function of two-dimensional spatial position and time. In particular, the overarching goal is to establish a unified approach to representation and analysis of temporal image dynamics that is broadly applicable to the diverse phenomena in the natural world as captured in two-dimensional intensity images. Previous research largely has approached the analysis of visual dynamics by appealing to representations based on image motion. Although of obvious importance, motion represents a particular instance of the myriad spatiotemporal patterns observed in image data. A generative model centred on the concept of spacetime orientation is proposed. This model provides a unified framework for understanding a broad set of important spacetime patterns. As a consequence of this analysis, two new classes of patterns are distinguished that have previously not been considered directly in terms of their constituent spacetime oriented structure, namely multiplicative motions (e.g., translucency) and stochastic-related phenomena (e.g.,
Endoscopie Techniques in Spinal Surgery (1997)

Minimally invasive techniques are becoming more widespread in the surgical subspecialties. Standard open surgical procedures are being modified to become less invasive, with the hopes of decreased recovery time, lessened morbidity, and ultimately, cost savings. Improvements in technology have allowed the surgeon to peer into body cavities and create potential spaces such as the retroperitoneum and the neuroforaminal space without the need for traditional extensile surgical approach. Improved fiberoptics, light sources, and the advent of the 3-chip camera and the 3-dimensional camera have resulted in improvements in visualization of the structures surrounding the spine. Although the goals of endoscopic surgery are to maintain or improve visualization and minimize the approach related trauma, procedures must also prove efficacious and safe with at least equivalent results compared with their open surgical counterpart. Not all procedures may be applicable to minimally invasive approaches and just because a procedure can be done does not mean that it should be done. Laparoscopic and thoracoscopic spine procedures also depend on the partnership of the spine surgeon with the thoracic or general surgeon with endoscopic experience to ensure patient safety. Proficiency in minimally invasive spinal techniques takes devotion and does not occur after taking minicourses. Practice with cadaver and in vivo models, preceptorship and proctorship training, and ultimately the teaching of these techniques in residency and spinal fellowship programs will undoubtedly lead to favorable outcomes and reduced medical expenditure. Preliminary results are encouraging for endoscopic spinal surgery, but further testing of these new techniques against conventional open procedures will be important in documenting not only the efficacy of the procedure, but also its value in patient satisfaction and cost.
Longitudinal immunological analyses reveal inflammatory misfiring in severe COVID-19 patients (2020)

Abstract Recent studies have provided insights into the pathogenesis of coronavirus disease 2019 (COVID-19) 1-4 . Yet, longitudinal immunological correlates of disease outcome remain unclear. Here, we serially analysed immune responses in 113 COVID-19 patients with moderate (non-ICU) and severe (ICU) disease. Immune profiling revealed an overall increase in innate cell lineages with a concomitant reduction in T cell number. We identify an association between early, elevated cytokines and worse disease outcomes. Following an early increase in cytokines, COVID-19 patients with moderate disease displayed a progressive reduction in type-1 (antiviral) and type-3 (antifungal) responses. In contrast, patients with severe disease maintained these elevated responses throughout the course of disease. Moreover, severe disease was accompanied by an increase in multiple type 2 (anti-helminths) effectors including, IL-5, IL-13, IgE and eosinophils. Unsupervised clustering analysis of plasma and peripheral blood leukocyte data identified 4 immune signatures, representing (A) growth factors, (B) type-2/3 cytokines, (C) mixed type-1/2/3 cytokines, and (D) chemokines that correlated with three distinct disease trajectories of patients. The immune profile of patients who recovered with moderate disease was enriched in tissue reparative growth factor signature (A), while the profile for those with worsened disease trajectory had elevated levels of all four signatures. Thus, we identified development of a maladapted immune response profile associated with severe COVID-19 outcome and early immune signatures that correlate with divergent disease trajectories.
Determination of a novel anticonvulsant drug GIZh-290 in rat plasma by liquid chromatography–tandem mass spectrometry (2022)

GIZh-290 is a new derivative of 4-phenylpyrrolidone, based on the structures of racetams that combine anticonvulsant and nootropic effects. The method of high-performance liquid chromatography with mass spectrometric detection was developed and validated for the quantitative determination of a new potential anticonvulsant compound GIZh-290 in rat blood plasma. For the extraction of the analyte, liquid-liquid extraction with diethyl ether was used. The method was validated based on its selectivity, linearity, accuracy, precision, and stability. The linearity of the method was confirmed by a high correlation coefficient of 0.999. The percent recovery of GIZh-290 from blood plasma was 79.2 %. The intra- and inter-batch accuracy were 19.32 and 12.14 %. It was found that blood plasma samples containing GIZh-290 can be stored at ambient temperature during the working day.
Tangent-ball techniques for shape processing (2009)

Shape processing defines a set of theoretical and algorithmic tools for creating, measuring and modifying digital representations of shapes. Such tools are of paramount importance to many disciplines of computer graphics, including modeling, animation, visualization, and image processing. Many applications of shape processing can be found in the entertainment and medical industries.&#13;\n&#13;\nIn an attempt to improve upon many previous shape processing techniques, the present thesis explores the theoretical and algorithmic aspects of a difference measure, which involves fitting a ball (disk in 2D and sphere in 3D) so that it has at least one tangential contact with each shape and the ball interior is disjoint from both shapes.&#13;\n&#13;\nWe propose a set of ball-based operators and discuss their properties, implementations, and applications. We divide the group of ball-based operations into unary and binary as follows:&#13;\n&#13;\nUnary operators include:&#13;\n* Identifying details (sharp, salient features, constrictions)&#13;\n* Smoothing shapes by removing such details, replacing them by fillets and roundings&#13;\n* Segmentation (recognition, abstract modelization via centerline and radius variation) of tubular structures&#13;\n&#13;\nBinary operators include:&#13;\n* Measuring the local discrepancy between two shapes&#13;\n* Computing the average of two shapes&#13;\n* Computing point-to-point correspondence between two shapes&#13;\n* Computing circular trajectories between corresponding points that meet both shapes at right angles&#13;\n* Using these trajectories to support smooth morphing (inbetweening)&#13;\n* Using a curve morph to construct surfaces that interpolate between contours on consecutive slices&#13;\n&#13;\nThe technical contributions of this thesis focus on the implementation of these tangent-ball operators and their usefulness in applications of shape processing. We show specific applications in the areas of animation and computer-aided medical diagnosis. These algorithms are simple to implement, mathematically elegant, and fast to execute.
Rolling-circle plasmids fromBacillus subtilis: complete nucleotide sequences and analyses of genes of pTA1015, pTA1040, pTA1050 and pTA1060, and comparisons with related plasmids from Gram-positive bacteria (1998)

Most small plasmids of Gram-positive bacteria use the rolling-circle mechanism of replication and several of these have been studied in considerable detail at the DNA level and for the function of their genes. Although most of the common laboratory Bacillus subtilis 168 strains do not contain plasmids, several industrial strains and natural soil isolates do contain rolling-circle replicating (RCR) plasmids. So far, knowledge about these plasmids was mainly limited to: (i) a classification into seven groups, based on size and restriction patterns; and (ii) DNA sequences of the replication region of a limited number of them. To increase the knowledge, also with respect to other functions specified by these plasmids, we have determined the complete DNA sequence of four plasmids, representing different groups, and performed computer-assisted and experimental analyses on the possible function of their genes. The plasmids analyzed are pTA1015 (5.8 kbp), pTA1040 (7.8 kbp), pTA1050 (8.4 kbp), and pTA1060 (8.7 kbp). These plasmids have a structural organization similar to most other known RCR plasmids. They contain highly related replication functions, both for leading and lagging strand synthesis. pTA1015 and pTA1060 contain a mobilization gene enabling their conjugative transfer. Strikingly, in addition to the conserved replication modules, these plasmids contain unique module(s) with genes which are not present on known RCR plasmids of other Gram-positive bacteria. Examples are genes encoding a type I signal peptidase and genes encoding proteins belonging to the family of response regulator aspartate phosphatases. The latter are likely to be involved in the regulation of post-exponential phase processes. The presence of these modules on plasmids may reflect an adaptation to the special conditions to which the host cells were exposed.
A Robust 6-D Pose Tracking Approach by Fusing a Multi-Camera Tracking Device and an AHRS Module (2021)

Optical tracking technique based on multiple cameras is widely employed in robots, virtual reality, and industrial measurements due to its excellent position accuracy. However, its 6-D tracking practicability is impaired by the line-of-sight issue and the complicated tracker based on multiple markers. Hence, we implemented a custom-built hybrid tracking system consisting of four-camera equipment and a 6-D tracker comprising a single optical marker and an automatic heading reference system (AHRS). AHRS provides the 3-D orientation of the tracked object directly and compensates for the 3-D position when the optical tracking is occluded. A two-stage cascaded adaptive unscented Kalman filtering (CAUKF) was proposed to enhance real-time fusion tracking performance. The CAUKF not only provides a reliable frequency enhancement solution for the optical tracking adapting to various frequencies, but also improves the continuality of prediction on fusion state variables and the corresponding covariance matrix, which helps initialize the occlusion tracking accurately. When an occlusion occurs, a learning-based adaptive unscented Kalman filter (LAUKF) module can adaptively adjust noise estimation matrices in the unscented transformation according to the AHRS data, thereby significantly reducing the position estimation error. Experimental results reveal that the proposed tracking approach achieved 6-D tracking at 100 Hz with 0.32-mm position error (mean absolute error) and 0.1° orientation error. This study furnishes a novel implementation method for the full 6-D pose tracking with a simplified tracker structure and improved accuracy, continuity, and stability.
Data Acquisition at CERN: A Future Challenge (2016)

How did the universe look in the first moments after the Big Bang? Why does matter dominate over antimatter? What are the fundamental particles that make up the world as we see it today? To find answers to those and similar questions, the European Organization for Nuclear Research (CERN) is operating the world's largest particle accelerator, the Large Hadron Collider (LHC). The LHC accelerates protons to a velocity close to the speed of light and makes hundreds of them collide. The circumstances shortly after those collisions are representative to the universe's conditions only moments after the Big Bang. By analyzing thousands of such collisions, a steps can be made toward answering the previous questions.
Understanding COVID-19 Pathophysiology: What Defines Progress? (2021)

TEST 02 - Elsevier's Scopus, the largest abstract and citation database of peer-reviewed literature. Search and access research from the science, technology, medicine, social sciences and arts and humanities fields.
An Unusual Ocular Finding Associated With Chromosome lq Deletion Syndrome (1986)

To the Editor.— We report an unusual ocular finding associated with the chromosome lq deletion syndrome in a full-term black girl for whom there was no family history of congenital anomalies, fetal wastage, consanguinity, or drug ingestion. The infant was overtly microcephalic (third percentile) with a sloping forehead, metopic sutures open to the brow, and a large posterior fontanel. She had a low anterior hair line, depressed nasal bridge, bulbous nose, thin down-turned lips, prominent philtrum, malformed ears, and a webbed neck.
An Equalization Method based on W-KNN for PON with PAM4 (2019)

We proposed a machine learning equalization technique based on weighted KNN for 50Gbps PAM4 PON systems. The simulation results show that 35dB loss budget is achieved by Weighted-KNN equalization technique.
Surface Structuring of Synthetic Fibers by UV Laser Irradiation Simulation of the Temperature Profile at the Polymer Surface (2000)

Abstract An investigation of the mechanism of the UV laser induced temperature field in a PETP-polymer is presented. We discuss a specific mathematical solution of the heat equation for large-area (uniform) irradiation on the PETP-substrate in relation to laser and substrate parameters. Depending on the order of magnitude of the absorption coefficient of the polymer for different UV-light surface temperatures of approx. 16,000°C (193 nm), 11,000°C (248 nm) and 520°C (308 nm) are calculated by simulation at the end of the laser pulse. The temperature input within the polymer layer is limited to only a very small penetration depth (< 0.4 μm at 193 nm, 248 nm and<8 μm for 308 nm). Besides the effect of surface structuring, material ablation processes also occur simultaneously during laser irradiation. Theoretically calculated results of the material removal depth on the PETP-polymer agree largely with the experiments.
Myocardial Injury After Noncardiac Surgery (MINS) in Vascular Surgical Patients (2017)

Approximately 1 in 5 patients experienced MINS after vascular surgery. MINS was independently associated with 30-day mortality. The majority of patients with MINS were asymptomatic and would have gone undetected without routine postoperative troponin measurement.
Capacitor Condition Monitoring Based on an Adaptive Observer of the Low-Frequency Capacitor Voltage Ripples for Modular Multilevel Converters (2019)

Capacitor condition monitoring in modular multilevel converters (MMCs) has become a must in some critical industry applications. This is because capacitors represent a vulnerable component in power electronic converters, and MMCs usually integrate hundreds of them. This paper presents a new submodule (SM) capacitor condition monitoring method for MMCs based on an adaptive observer of the low-frequency capacitor voltage ripple. The proposed method does not require any additional hardware cost, it is intuitively simple, and computationally efficient. Moreover, it can provide capacitor condition monitoring of all the SMs in the converter simultaneously without affecting its operation. The operation principle of the proposed method consists in using the error between the measured SM capacitor voltage and its estimated value to derive the parametric uncertainty between the practical system SM capacitance and its nominal value assumed in the observer model. Therefore, if the SM capacitor exhibits a variation in its capacitance value, the proposed algorithm processes the consequent error signal to determine the actual capacitance. Subsequently, the observer model is updated, which brings the estimation error towards zero in the steady state. Simulation results on a 800-W MMC confirm the effectiveness of the proposed method.
An ion-channel-containing model membrane: structural determination by magnetic contrast neutron reflectometry (2009)

To many biophysical characterisation techniques, biological membranes appear as two-dimensional structures with details of their third dimension hidden within a 5 nm profile. Probing this structure requires methods able to discriminate multiple layers a few Ångströms thick. Given sufficient resolution, neutron methods can provide the required discrimination between different biochemical components, especially when selective deuteration is employed. We have used state-of-the-art neutron reflection methods, with resolution enhancement via magnetic contrast variation to study an oriented model membrane system. The model is based on the Escherichia coli outer membrane protein OmpF fixed to a gold surface via an engineered cysteine residue. Below the gold is buried a magnetic metal layer which, in a magnetic field, displays different scattering strengths to spin-up and spin-down neutrons. This provides two independent datasets from a single biological sample. Simultaneous fitting of the two datasets significantly refines the resulting model. A β-mercaptoethanol (βME) passivating surface, applied to the gold to prevent protein denaturation, is resolved for the first time as an 8.2 ± 0.6 Å thick layer, demonstrating the improved resolution and confirming that this layer remains after OmpF assembly. The thiolipid monolayer (35.3 ± 0.5 Å), assembled around the OmpF is determined and finally a fluid DMPC layer is added (total lipid thickness 58.7 ± 0.9 Å). The dimensions of trimeric OmpF in isolation (53.6 ± 2.5 Å), after assembly of lipid monolayer (57.5 ± 0.9 Å) and lipid bilayer (58.7 ± 0.9 Å), are precisely determined and show little variation.
Reflective Optical Fiber Refractometer Based on Long-Period Grating Tailored Active Bragg Grating (2015)

A reflective optical fiber refractometer based on a long-period grating (LPG) followed by an active fiber Bragg grating (AFBG) is proposed. The AFBG is inscribed in a short section of cobalt-doped optical fiber, which can be heated by pump laser and acts as a sensing signal indicator. The LPG functions as a bridge between the surrounding refractive index (RI) and the AFBG reflection, tailoring the power of pump laser for heating. Experimental results demonstrate that the wavelength response of this fiber grating-based reflective refractometer can be easily adjusted, and a significantly improved sensitivity within the RI range from 1.3403 to 1.4654 has been achieved.
Late-night salivary cortisol (LNSC) levels in a Phase III study of long-acting pasireotide in patients with Cushing's disease (CD) (2018)

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)