gebhart/patrepeval huggingface dataset:
Invasion of cultured human epithelial cells by Klebsiella pneumoniae isolated from the urinary tract (1997)

The mechanisms which enable entry into cultured human epithelial cells by Klebsiella pneumoniae were compared with those of Salmonella typhi Ty2. K. pneumoniae 3091, isolated from a urine sample of a patient with a urinary tract infection, invaded human epithelial cells from the bladder and ileocecum and persisted for days in vitro. Electron microscopic studies demonstrated that K. pneumoniae was always contained in endosomes. The internalization mechanism(s) triggered by K. pneumoniae was studied by invasion assays conducted with different inhibitors that act on prokaryotic and eukaryotic cell structures and processes. Chloramphenicol inhibition of bacterial uptake revealed that bacterial de novo protein synthesis was essential for efficient invasion by K. pneumoniae and S. typhi. Interference with receptor-mediated endocytosis by g-strophanthin or monodansylcadaverine and inhibition of endosome acidification by monensin reduced the number of viable intracellular K. pneumoniae cells, but not S. typhi cells. The depolymerization of microfilaments by cytochalasin D inhibited the uptake of both bacteria. Microtubule depolymerization caused by colchicine, demecolcine, or nocodazole and the stabilization of microtubules with taxol reduced only the invasion ability of K. pneumoniae. S. typhi invasion was unaffected by microtubule depolymerization or stabilization. These data suggest that the internalization mechanism triggered by K. pneumoniae 3091 is strikingly different from the solely microfilament-dependent invasion mechanism exhibited by many of the well-studied enteric bacteria, such as enteroinvasive Escherichia coli, Salmonella, Shigella, and Yersinia strains.
Synthesis and Properties of 6‐Amino‐7‐hetaryl‐5‐R‐5H‐pyrrolo[2,3‐b]pyrazine‐2,3‐dicarbonitriles. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Chronology and morphology of temperature‐dependent sex determination (1991)

Temperature sensitivity and gonadal differentiation were studied in a turtle, Trachemys scripta, with temperature-dependent sex determination. Sex determination was sensitive to both the duration and magnitude of incubation temperature. Temperature exerted an "all or none" effect on the ovarian or testicular nature of most gonads, but affected the length of ovaries in a graded fashion. Collectively, the results indicate that sex determination is controlled by the quantitative effect of temperature during a period beginning prior to (histologically detectable) sexual differentiation and extending to a time when initial sex specific changes are evident in the gonads.
Las bombas de calor: Factores energéticos, ambientales y económicos (2002)

Ossification of the posterior longitudinal ligament of the thoracic spine (T-OPLL) causes symptoms including leg and back pain, and motor and sensory deficits. This study retrospectively reviewed 32 patients who initially underwent posterior decompression with instrumented fusion (PDF) for T-OPLL between 2001 and 2012, with 20 qualifying for the final analysis after applying exclusion criteria. Exclusions included unknown preoperative neurological findings, follow-up less than 10 years, or prior spinal surgeries at other levels. Outcomes were assessed using the Japanese Orthopedic Association (JOA) score, recovery rate, and kyphotic angle. The average preoperative JOA score of 3.6 improved to 7.4 at 1 year post-surgery and remained at 7.4 at 10 years, with a recovery rate of 52%. The kyphotic angle at T4-12 increased from 26 degrees preoperatively to 29 degrees postoperatively and to 37 degrees at 10 years. At the fused levels, the angle remained at 26 degrees immediately post-operation and increased to 32 degrees at 10 years. Forty percent of patients required additional surgery, primarily for conditions related to cervical OPLL, such as myelopathy, or lumbar OPLL, such as radiculopathy, or cauda equina syndrome. In conclusion, PDF effectively reduces T-OPLL symptoms over the long term, but the high rate of additional surgeries calls for careful patient follow-up.
Preface (2002)

This book is intended for all those who struggle with Asperger syndrome in their everyday life. They are many. They range from the people with the diagnosis, and their near and dear, through clinicians faced with the problems of understanding, investigating and intervening in ‘a disorder’ that they heard little about during their education, but read about in the newspapers and magazines almost on a weekly basis.
Improved usability of the minimal model of insulin sensitivity based on an automated approach and genetic algorithms for parameter estimation (2007)

Minimal model analysis of glucose and insulin data from an IVGTT (intravenous glucose tolerance test) is widely used to estimate insulin sensitivity; however, the use of the model often requires intervention by a trained operator and some problems can occur in the estimation of model parameters. In the present study, a new method for minimal model analysis, termed GAMMOD, was developed based on genetic algorithms for the estimation of model parameters. Such an algorithm does not require the fixing of initial values for the parameters (that may lead to unreliable estimates). Our method also implements an automated weighting scheme not requiring manual intervention of the operator, thus improving the usability of the model. We studied a group of 170 women with a history of previous gestational diabetes. Results obtained by GAMMOD were compared with those obtained by MINMOD (a traditional gradient-based algorithm for minimal model analysis). Insulin sensitivity by GAMMOD was (3.86+/-0.19) compared with (4.33+/-0.20) x 10(-4) micro-units.ml(-1) x min(-1) by MINMOD; glucose effectiveness was 0.0236+/-0.0005 compared with 0.0229+/-0.0005 min(-1) respectively. The difference in the estimation by the two methods was within the precision expected for such metabolic parameters and is probably of no clinical relevance. Moreover, both the coefficient of variation of the estimated parameters and the error of fit were generally lower in GAMMOD, despite the fact that it does not require manual intervention. In conclusion, the GAMMOD approach for parameter estimation in the minimal model provides a reliable estimation of the model parameters and improves the usability of the model, thus facilitating its further use and application in a clinical context.
Arthroscopic Treatment of Osteochondral Lesions of the Talus With Microfracture and Platelet‐Rich Plasma‐Infused Micronized Cartilage Allograft (2020)

Osteochondral lesions of the talus (OLTs) are difficult to treat. Arthroscopic microfracture augmented with micronized cartilage (BioCartilage; Arthrex, Naples, FL) and platelet-rich plasma is emerging as a treatment for moderate-sized, well-contained full-thickness OLTs. This treatment may provide superior histologic results and is less technically demanding and yields less morbidity than an open osteochondral allograft or autograft transfer. This technique guide presents the senior author's preferred strategy for treatment of a moderate-sized OLT with arthroscopic microfracture and placement of micronized cartilage and platelet-rich plasma.
Effects of gonadotropin-releasing hormone administration or a controlled internal drug-releasing insert after timed artificial insemination on pregnancy rates of dairy cows (2016)

This study investigated the effects of gonadotrophin-releasing hormone (GnRH) administration (Experiment 1) and a controlled internal drug-releasing (CIDR) insert (Experiment 2) after timed artificial insemination (TAI) on the pregnancy rates of dairy cows. In Experiment 1, 569 dairy cows that underwent TAI (day 0) following short-term synchronization with prostaglandin F<sub>2α</sub> were randomly allocated into two groups: no further treatment (control, n = 307) or injection of 100 µg of gonadorelin on day 5 (GnRH, n = 262). In Experiment 2, 279 dairy cows that underwent TAI (day 0) following Ovsynch were randomly allocated into two groups: no further treatment (control, n = 140) or CIDR insert treatment from days 3.5 to 18 (CIDR, n = 139). The probability of pregnancy following TAI did not differ between the GnRH (34.4%) and control (31.6%, <i>p</i> > 0.05) groups. However, the probability of pregnancy following TAI was higher (odds ratio: 1.74, <i>p</i> < 0.05) in the CIDR group (51.1%) than in the control group (39.3%). Overall, CIDR insert treatment at days 3.5 to 18 increased pregnancy rates relative to non-treated controls, whereas a single GnRH administration on day 5 did not affect the pregnancy outcomes of dairy cows.
Efficiency Improvement of the Parity-Time-Symmetric Wireless Power Transfer System for Electric Vehicle Charging (2020)

Wireless power transfer (WPT) is a promising technology for charging electric vehicles (EVs) due to its intelligence, convenience, and reliability. However, achieving high efficiency and high power with wide misalignment tolerance is still a major challenge in commercializing wireless charging technology in EVs. Here, a novel parity-time-symmetric-based WPT (PT-based WPT) system with multiple decoupled receiving coils is developed to address the above issues. The circuit model of this PT-based WPT system is established. Our theoretical analysis shows that the output power and transfer efficiency of the proposed system also remain constant over a coupling coefficient variation. More importantly, compared to the conventional one-receiving-coil PT-based WPT system, the use of multiple receiving coils can effectively improve the transfer efficiency and reduce the current stress of system. In order to evaluate the proposed WPT system, a 1-kW laboratory prototype is built and tested. Experimental results show that a stable of power transfer is maintained with constant transfer efficiency of 96.1% within a range of 100-200-mm air gap. In particular, the same transfer efficiency is achieved at 100-mm air gap within a horizontal tolerance of 240 mm, while a horizontal tolerance of 100 mm at 200-mm air gap.
Supplementary Figure 2 from Distinct Effects of Ligand-Induced PDGFRα and PDGFRβ Signaling in the Human Rhabdomyosarcoma Tumor Cell and Stroma Cell Compartments (2023)

&lt;p&gt;PDF file - 46K, Gene set enrichment analysis associates PDGF-D expression with genes involved in ECM organization, leukocyte transendothelial migration and cell adhesion in human RMS tumors. A, biological processes associated with genes expressed in high PDGF-D expressing tumors compared to low PDGF-D expressing tumors, based on GO annotations. The first 17 categories that reached a p-value &lt;0.01 are shown. B, pathways associated with genes expressed in high PDGF-D expressing tumors compared to low PDGF-D expressing tumors, using KEGG annotations. The first 11 categories that reached a p-value &lt;0.01 are shown.&lt;/p&gt;
Multitask Instruction-based Prompting for Fallacy Recognition (2023)

Fallacies are used as seemingly valid arguments to support a position and persuade the audience about its validity. Recognizing fallacies is an intrinsically difficult task both for humans and machines. Moreover, a big challenge for computational models lies in the fact that fallacies are formulated differently across the datasets with differences in the input format (e.g., question-answer pair, sentence with fallacy fragment), genre (e.g., social media, dialogue, news), as well as types and number of fallacies (from 5 to 18 types per dataset). To move towards solving the fallacy recognition task, we approach these differences across datasets as multiple tasks and show how instruction-based prompting in a multitask setup based on the T5 model improves the results against approaches built for a specific dataset such as T5, BERT or GPT-3. We show the ability of this multitask prompting approach to recognize 28 unique fallacies across domains and genres and study the effect of model size and prompt choice by analyzing the per-class (i.e., fallacy type) results. Finally, we analyze the effect of annotation quality on model performance, and the feasibility of complementing this approach with external knowledge.
Shadowgraphic and spectroscopic diagnostics of the nanosecond underwater discharges (2011)

International audience
On the Stability of Biped Locomotion (1970)

The stability of legged machines in locomotion is considered. The general machine has a rigid body to which legs are attached. Locomotion is performed on level smooth surfaces.
Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity (2005)

A lipase from Pseudomonas aeruginosa was subjected to directed molecular evolution for increased amide-hydrolyzing (amidase) activity. A single round of random mutagenesis followed by screening for hydrolytic activity for oleoyl 2-naphthylamide as compared with that for oleoyl 2-naphthyl ester identified five mutants with 1.7-2.0-fold increased relative amidase activities. Three mutational sites (F207S, A213D and F265L) were found to affect the amidase/esterase activity ratios. The combination of these mutations further improved the amidase activity. Active-site titration using a fluorescent phosphonic acid ester allowed the molecular activities for the amide and the ester to be determined for each mutant without purification of the lipase. A double mutant F207S/A213D gave the highest molecular activity of 1.1 min(-1) for the amide, corresponding to a 2-fold increase compared with that of the wild-type lipase. A structural model of the lipase indicated that the mutations occurred at the sites near the surface and remote from the catalytic triad, but close to the calcium binding site. This study is a first step towards understanding why lipases do not hydrolyze amides despite the similarities to serine proteases in the active site structure and the reaction mechanism and towards the preparation of a general acyl transfer catalyst for the biotransformation of amides.
Cold Rolling Technique for Eliminating Cutting Process in Manufacturing Precise Product Using Non-Heat-Treated Micro Alloys (2005)

In general micro alloy steel have the higher strength relative to conventional steels, which limits the utilization of conventional plastic forming processes. Incremental forming processes are more suitable for cold forming of such a high strength material. In particular, cold cross wedge rolling (CWR) can be a potential tool to fabricate axi-symmetric components with multi steps using high strength micro alloy steel. Obviously, optimization of die shape design is a crucial factor to apply cold cross wedge rolling to micro alloy steels. In this regards, a simulation-based process design using an elasto-plastic FEM has been carried out in order to obtain an optimum die shape for cold cross wedge rolling in this paper. Analysis results provided that the stretching angle and the shoulder angle at knifing and guiding zones were significant parameters for the stable forming process. It was demonstrated that proper stretching and shoulder angles reduced an excessive slip between a work piece and die in CWR process despite the condition of the low friction coefficient.
Supplementary Figures 1-4 from Marked Genomic Differences Characterize Primary and Secondary Glioblastoma Subtypes and Identify Two Distinct Molecular and Clinical Secondary Glioblastoma Entities (2023)

Supplementary Figures 1-4 from Marked Genomic Differences Characterize Primary and Secondary Glioblastoma Subtypes and Identify Two Distinct Molecular and Clinical Secondary Glioblastoma Entities
Endoglin in liver fibrosis (2011)

Liver fibrosis occurs in most types of chronic liver diseases and is characterized by excessive accumulation of extracellular matrix proteins, leading to disruption of tissue function and eventually organ failure. Transforming growth factor (TGF)-β represents an important pro-fibrogenic factor and aberrant TGF-β action has been implicated in many disease processes of the liver. Endoglin is a TGF-β co-receptor expressed mainly in endothelial cells that has been shown to differentially regulates TGF-β signal transduction by inhibiting ALK5-Smad2/3 signalling and augmenting ALK1-Smad1/5 signalling. Recent reports demonstrating upregulation of endoglin expression in pro-fibrogenic cell types such as scleroderma fibroblasts and hepatic stellate cells have led to studies exploring the potential involvement of this TGF-β co-receptor in organ fibrosis. A recent article by Meurer and colleagues now shows that endoglin expression is increased in transdifferentiating hepatic stellate cells in vitro and in two different models (carbon tetrachloride intoxication and bile duct ligation) of liver fibrosis in vivo. Moreover, they show that endoglin overexpression in hepatic stellate cells is associated with enhanced TGF-β-driven Smad1/5 phosphorylation and α-smooth muscle actin production without altering Smad2/3 signaling. These findings suggest that endoglin may play an important role in hepatic fibrosis by altering the balance of TGF-β signaling via the ALK1-Smad1/5 and ALK-Smad2/3 pathways and raise the possibility that targeting endoglin expression in transdifferentiating hepatic stellate cells may represent a novel therapeutic strategy for the treatment of liver fibrosis.
Plasma pharmacokinetics of warfarin enantiomers in cats (2000)

The purpose of this study was to determine the dispositions of S-warfarin and R-warfarin in normal cats following intravenous and oral administrations of racemic warfarin. Citrated blood samples were collected from 10 cats prior to and at times 5, 15, and 30 min, 1, 2, 3, 4, 5, 6, 12, 24, 36, 48, 72, 96, and 120 h following a single intravenous bolus of 0.5 mg/kg of racemic warfarin. After a 21-day washout period, samples were then similarly collected in three groups of four cats for 120 h following oral administration of 0.1, 0.25, and 0.5 mg/kg racemic warfarin. S-warfarin and R-warfarin were detected using a high-performance liquid chromatography assay validated for cat plasma. Drug concentration-time curves were subjected to non-compartmental analysis. Median pharmacokinetic parameters associated with the intravenous administration of 0.5 mg/kg racemic warfarin were as follows: t1/2 (S:28.2, R:18.3 h), area under the plasma concentration-time curve (AUC; S:33.0, R:24.6 h*microg/mL), area under the moment curve (AUMC; S:1889, R:527.8 h*h*microg/mL), and mean residence time (MRT; S:38.7, R:20.9 h). For each parameter, S-warfarin was significantly different from R-warfarin (P<0.05). Warfarin was absorbed rapidly after oral administration, and the dosage did not affect the time to maximum concentration (S:0.87, R:0.75 h). Oral dosage significantly influenced maximum plasma concentration (ng/mL, S:1267, R:1355 at 0.5 mg/kg; S:614.9, R:679.4 at 0.25 mg/kg; S:250.5, R:367.6 at 0.1 mg/kg), AUC (h*microg/mL, S:45.12, R:30.91 at 0.5 mg/kg; S:22.98:, R:18.99 at 0.25 mg/kg; S:3.922, R:3.570 at 0.1 mg/kg) and AUMC (h*h*microg/mL, S:2135, R:1062 at 0.5 mg/kg; S:943.1, R:599.9 at 0.25 mg/kg; S:132.2, R:59.03 at 0.1 mg/kg), but not t1/2 (S:23.5, R:11.6 h) nor MRT (S:26.3, R:13.5 h). Both warfarin enantiomers were highly (>96.5%) protein-bound. Quantitation of the warfarin content in commercially available tablets indicated an unequal distribution of the drug throughout the tablet.
ChemInform Abstract: Transformation of Dialkynylelementa Species to Exoalkylidene‐1‐ elementacyclobut‐2‐enyl Ligands: A Novel Route to Unsaturated Four‐ Membered Heterocycles. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Synthesis and Purification of Oligonucleotides (1998)

This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.
Mechanical Properties of Small-scale Laminated Wood Composite Poles: Effects Of Taper and Webs (2006)

Laminated hollow wood composite poles represent an efficient utilization of the timber resource and a promising alternative for solid wood poles that are commonly used in the power transmission and telecommunication lines.The objective of this study was to improve the performance of composite poles by introducing the bio-mimicry concept into the design of hollow wood composite poles.Five laminated hollow wood composite poles with taper and plywood-made webs, acting like the nodes in the bamboo, were made and tested in cantilevered static bending.Results indicated that node-like webs had a positive effect on the integrity, static bending properties, and shear resistance of the members tested, and their strength performance is comparable to that of the solid wood composite poles.However, the laminated hollow wood composite poles with taper showed slightly lower resistance to horizontal shear as compared to the members without taper.
Spin transport properties of 3d transition metal(ii) phthalocyanines in contact with single-walled carbon nanotube electrodes (2010)

The spin transport properties of a series of 3d transition metal(ii) phthalocyanines (MPc, M = Mn, Fe, Co, Ni, Cu and Zn) sandwiched between two semi-infinite armchair single-walled carbon nanotube electrodes are investigated by using a self-consistent ab initio approach that combines the non-equilibrium Green's function formalism with spin density functional theory. Our calculations show that among the six molecules only MnPc and FePc can act as nearly perfect spin filters and at the same time have a large transmission around the Fermi level. This is dominated by the highest occupied molecular orbital (HOMO) of the corresponding MPc molecule. In contrast to the other four MPc molecules, whose HOMO is the a(1u) orbital located over the Pc ring, the HOMO of MnPc and FePc is a doubly degenerate pi-type orbital composed of the 3d(xz) and 3d(yz) atomic orbitals of the metal center. The spin polarization of MnPc and FePc is independent of the size of the SWCNT electrodes and can be tuned by chemisorption at the metal center, demonstrating that MPc and carbon nanotubes are a promising materials platform for applications in molecular spintronics.
Complex Reconstruction for the Treatment of Dorsolateral Peritalar Subluxation of the Foot. Early Results After Distraction Arthrodesis of the Calcaneocuboid Joint in Conjunction with Stabilization of, and Transfer of the Flexor Digitorum Longus Tendon to, the Midfoot to Treat Acquired Pes Planovalgus in Adults* (1999)

Despite the high prevalence of postoperative complications, most of our patients were satisfied with the result of the procedure after the short duration of follow-up. We believe that the relief of pain and the restoration of function achieved through effective correction of the severe pes planovalgus deformity account for the satisfactory outcomes in our patients.
Cryoanalgesia (1981)

The introduction of cryoanalgesia has reawakened interest in the therapeutic use of cold as a means of producing pain relief. The historical background to both local cooling and nerve freezing is recorded and the advances since the introduction of cryoprobes in 1961 are described. The possible mechanisms of freeze damage, the physical changes occurring in tissues as a result of ice crystal formation and the influence of the probe design on the size of individual cryolesions are all discussed. Gas expansion cryoprobes are described and the application of nerve freezing in the control of both post operative and chronic pain reported.