gebhart/patrepeval huggingface dataset:
Endothelium-Mediated Hepatocyte Recruitment in the Establishment of Liver-like Tissue In Vitro (2006)

A major goal of liver tissue engineering is to understand how the constituent cell types interact to achieve liver-specific structure and function. Here we show that hepatocytes migrate toward and adhere to endothelial vascular structures formed on Matrigel in vitro, and that hepatocyte recruitment is dependent on endothelium-derived hepatocyte growth factor. The hepatocyte-decorated endothelial vascular structures resemble In vivo sinusoids containing plate-like structures, bile canaliculi, and a lumen. The sinusoid-like structures retained cytochrome P450 expression and activity, in addition to stable albumin expression and secretion rate for over 2 months in vitro. The stability of the sinusoid-like structures was dependent on the presence of vimentin-positive fibroblasts in culture. The sinusoid-like structures formed by hepatocytes and pure populations of endothelial cells collapsed after 10 days in culture. In contrast, culture of hepatocytes with fibroblast-contaminated human dermal microvascular endothelial cells or a combination of human umbilical vein endothelial cells and normal human dermal fibroblasts resulted in stable sinusoid-like structures surrounded by a fibroblastic capsule that maintained liver specific functions for several months in vitro. These results demonstrate that specification of endothelial cell position ultimately determines hepatocyte position in vitro, suggesting that similar interactions might occur In vivo. The novelty of the culture's sinusoid-like organization and long-term function suggest a new model for the study of liver toxicity, ischemia/reperfusion injury, and fibrosis.
Stress controlled shallow trench isolation technology to suppress the novel ant-isotropic impurity diffusion for 45nm-node high-performance CMOSFETs (2005)

The most suitable STI filling process has been developed for 45nm-node SoC platform. We found that the stress induced anti-isotropic impurity diffusion, which causes the Vth lowering. This novel phenomenon has been controlled by optimizing the SOD/HDP-CVD hybrid STI filling structure. At the same time, 20% drive current improvements of nFET and pFET have been obtained.
Fgf15 is required for proper morphogenesis of the mouse cardiac outflow tract (2005)

Evidence in animal models indicates that signaling networks functioning in the developing pharyngeal arches regulate stereotyped processes critical for proper development of the aortic arch and cardiac outflow tract. Here, we describe the phenotype of mice lacking fibroblast growth factor 15 (Fgf15), which encodes a secreted signaling molecule expressed within the developing pharyngeal arches. Homozygous Fgf15 mutants present heart defects consistent with malalignment of the aorta and pulmonary trunk. These defects correlate with early morphological defects of the outflow tract due to aberrant behavior of the cardiac neural crest. We demonstrate that Fgf15 expression within the pharyngeal arches is unaltered by a loss of Tbx1, a key regulator of pharyngeal arch development implicated in DiGeorge syndrome. In addition, Fgf15 and Tbx1 do not interact genetically, suggesting that Fgf15 operates through a pathway independent of Tbx1. These studies reveal a novel role of Fgf15 during development of the cardiac outflow tract.
Two Types of Mixed Orthogonal Frequency Division Multiplexing (X-OFDM) Waveform for Optical Wireless Communication (2020)

The optical wireless communication (OWC) with the intensity modulation (IM), requires the modulated radio frequency (RF) signal to be real and non-negative. To satisfy the requirements, this paper proposes two types of mixed orthogonal frequency division multiplexing (X-OFDM) waveform. The Hermitian symmetry (HS) character of the sub-carriers in the frequency domain, guarantees the signal in the time domain to be real, which reduces the spectral efficiency to $1/2$. For the odd sub-carriers in the frequency domain, the signal in the time domain after the inverse fast fourier transform (IFFT) is antisymmetric. For the even sub-carriers in the frequency domain, the signal in the time domain after the IFFT is symmetric. Based on the antisymmetric and symmetric characters, the two types of X-OFDM waveform are designed to guarantee the signal in the time domain to be non-negative, where the direct current (DC) bias is not needed. With $N$ sub-carriers in the frequency domain, the generated signal in the time domain has $3N/2$ points, which further reduces the spectral efficiency to $1/3 = 1/2 \times 2/3$. The numerical simulations show that, the two types of X-OFDM waveform greatly enhance the power efficiency considering the OWC channel with the signal-dependent noise and/or the signal-independent noise.
Conceptual Clustering in Database Systems (1991)

Classes are an integral part of all semantic data models. Despite this, class formation in these data models is ad hoc due to the varied treatment of classes and because the issue of grouping instances into classes is considered an art rather than a science. It is the view of this paper that class formation be based on category theory through the use of an aJtribute-based purpose-dieected conceptual clustering technique. Several issues concerned with category theory, especially exception handling, are discussed. The emphasis in this approach is on reasoning at the instance level. Schema generation occurs as a result of conceptually clustering the underlying data instances and guiding this process by specifying a context in the form of a clustering seed. The use of this approach in the areas of schema integration, schema evolution and querying will be discussed. These facilities have been implemented on a database system based on the CANDIDE [3] semantic data model. CANDIDE is essentially an extended version of the tenn-subsumption languages known as the KL-ONE family of languages.
CRISPR/Cas9-mediated engineering of Escherichia coli for n-butanol production from xylose in defined medium (2019)

Butanol production from agricultural residues is the most promising alternative for fossil fuels. To reach the economic viability of biobutanol production, both glucose and xylose should be utilized and converted into butanol. Here, we engineered a dual-operon-based synthetic pathway in the genome of E. coli MG1655 to produce n-butanol using CRISPR/Cas9 technology. Further deletion of competing pathway followed by fed-batch cultivation of the engineered strain in a bioreactor with glucose-containing complex medium yielded 5.4 g/L n-butanol along with pyruvate as major co-product, indicating a redox imbalance. To ferment xylose into butanol in redox-balanced manner, we selected SSK42, an ethanologenic E. coli strain engineered and evolved in our laboratory to produce ethanol from xylose, for integrating synthetic butanol cassette in its genome via CRISPR/Cas9 after deleting the gene responsible for endogenous ethanol production. The engineered plasmid- and marker-free strain, ASA02, produced 4.32 g/L butanol in fed-batch fermentation in completely defined AM1-xylose medium.
Investigating the feasibility of cerebellar transcranial direct current stimulation to facilitate post-stroke overground gait performance in chronic stroke: a partial least-squares regression approach (2021)

Being retrospectively registered.
Rebound Pain in a Patient Taking Medical Marijuana for Phantom Limb Syndrome (P3.9-054) (2019)

N/A
Nonstoichiometry and the Long‐Range Cation Ordering in Crystals of (Na 1/2 Bi 1/2 ) TiO 3 (1994)

Crystals of (Na 1/2 Bi 1/2 )TiO 3 have grown by the flux technique and the Czochralski method. Nonstoichiometry, twin configurations, and long‐range cation ordering in the crystals have been investigated using X‐ray diffraction and an optical polarizing microscope. It has been found that nonstoichiometry was induced in the crystal grown by the Czochralski method owing to the volatilization of Bi‐rich phase during the crystal growth. This nonstoichiometry in the crystal resulted in less lattice distortion from cubic symmetry, a lower degree of cation ordering, and a larger domain width in twin configuration. Variations in twinning with temperature and isotropization have been investigated.
Conductimetric immunosensor based on poly(3,4-ethylenedioxythiophene) (2002)

A conductimetric reagentless immunosensor using the biospecific binding pair of goat antirabbit IgG and rabbit IgG has been designed and fabricated using poly (3,4-ethylenedioxythiophene) as the immobilization matrix-cumtransducer.
On-chip error correcting techniques for new-generation flash memories (2003)

In new-generation flash memories, issues such as disturbs and data retention become more and more critical as a consequence of reduced cell size and decreased oxide thickness. Furthermore, the progressive increase in the cell count within a single die tends to decrease device reliability. In particular, reliability issues turn out to be more critical in multilevel (ML) flash memories, due to the reduced spacing between adjacent programmed levels. It is therefore deemed that the use of on-chip error correction codes (ECCs) will gain widespread acceptance in large-capacity flash memories. ECCs for flash memories must have very fast and compact encoding/decoding circuitry so as to have a minimum impact on memory access time. The area penalty due to check cells must also be minimized. Moreover, specific codes must be developed for ML storage. This paper presents error control coding techniques and schemes for new-generation flash memories, focusing on ML devices. The basic concepts of error control coding are reviewed, and the on-chip ECC design procedure is analyzed. Dedicated codes such as polyvalent ECCs, able to correct data stored in ML memories working at a variable number of bits per cell, and bit-layer organized ECCs are described.
Lipschitz stability of a non-standard problem for the non-stationary transport equation via a Carleman estimate (2006)

The Lipschitz stability estimate for the non-stationary single-speed transport equation with lateral boundary data is obtained. The method of Carleman estimates is used. The uniqueness of the solution follows.
Hole Transport in Nonstoichiometric and Doped Wüstite (2012)

We propose ways to enhance the conductivity of wüstite (i.e., naturally p-type FeO), a visible-light-absorbing, inexpensive, abundant, and nontoxic potential alternative material for solar energy conversion devices. Unfortunately, the conversion efficiency of FeO is inhibited by its low hole conductivity. Increasing the iron vacancy concentration or adding p-type dopants improves FeO conductivity by increasing the number of holes; however, it is not known which strategy introduces larger energy traps that would hinder hole conductivity. Here we employ the small polaron model along with ab initio calculations on electrostatically embedded clusters to analyze the local trapping effects of iron vacancies and several substitutional p-type dopants that are soluble in FeO, including copper, nitrogen, lithium, and sodium, and also hydrogen as an interstitial dopant for comparison. We find that vacancies create stronger traps than dopants and that copper and nitrogen dopants form deeper traps than lithium, sodium, or hydrogen. Furthermore, hydrogen repels the hole and substantially decreases the trap formed by an iron vacancy. Because of the shallower traps formed compared to vacancies, lithium-, sodium-, or hydrogen-doped, nanostructured or alloyed FeO may be worth evaluating as a p-type semiconductor for solar energy conversion applications.
Expression of VEGF and angiopoietins-1 and -2 during ischemia-induced coronary angiogenesis (2003)

The mechanisms underlying coronary capillary growth in response to ischemia are undefined. We hypothesized that the expression of vascular endothelial growth factor (VEGF) and angiopoietin (Ang)/Tie-2 were involved in capillary growth as an adaptation to ischemia. To test this hypothesis we measured capillary density, and the expressions of VEGF, Ang-1, Ang-2, and the Tie-2 receptor and its phosphorylation state during repetitive episodes of myocardial ischemia in chronically instrumented canines. Repetitive episodes of ischemia were induced by multiple (once/hour; 8/day), brief (2 min) occlusions of the left anterior descending coronary artery for 1, 7, 14, or 21 days. A sham group received the same instrumentation as the experimental groups but not the occlusion protocol. Collateral blood flow (microspheres) progressively increased from 9 +/- 3 to 83 +/- 10 ml. min-1. 100 g-1 on day 21. Capillary density increased at day 7 from 2378 +/- 53 (sham) to 2962 +/- 60/mm2, but it decreased to 2594 +/- 39/mm2 at day 21. Both VEGF and Ang-2 expression in myocardial interstitial fluid (Western analyses) peaked at day 3 of the repetitive occlusions but waned thereafter. In contrast the expression of Ang-1 remained relatively constant at all times in the occlusion groups. In shams, the expression of VEGF and Ang-2 was low and constant at all times. Tie-2 phosphorylation myocardial decreased decreased at day 7 but increased at 21 days of occlusions (P < 0.05). Our results indicate that capillary density was augmented by myocardial ischemia, but after development of collaterals and restoration of flow to the ischemic zone, capillary density returned to control levels. The change in capillary density paralleled with VEGF and Ang-2 expression but was inversely related to Tie-2 phosphorylation. We speculate the coronary angiogenesis is a coordinated event involving the expression of both VEGF and Ang-2 and that therapeutic angiogenic strategies may ultimately require treatment with more than a single factor.
Pharmacokinetics of Ampicillin and Sulbactam in Pediatric Patients (1999)

Intravenous ampicillin-sulbactam is effective in the treatment of various infections in adults, but little is known about the pharmacokinetics (PK) of ampicillin-sulbactam in children. The objective of this study was to determine the PK of ampicillin and sulbactam in pediatric patients with intra-abdominal infection, skin and/or skin structure infection, or periorbital-preseptal and facial cellulitis. Intravenous ampicillin and sulbactam (2:1), 40 to 80 mg/kg of body weight, were given every 6 h for 2 to 6 days to 28 pediatric patients. The ages ranged from 1 to 6 years for 10 patients, 6.1 to 10 years for 9 patients, and 10.1 to 12 years for 9 patients. Multiple blood samples were obtained and analyzed for ampicillin and sulbactam in plasma and serum by high-performance liquid chromatography. The mean maximum concentration of drug in serum ranged from 177 to 200 micrograms/ml for ampicillin and 82 to 102 micrograms/ml for sulbactam in the three age groups. The mean total clearance, steady-state distribution volume, and half-life were 4.76 ml/min/kg, 0.32 liter/kg, and 0.77 h, respectively, for ampicillin and 4.95 ml/min/kg, 0.34 liter/kg, and 0.81 h, respectively, for sulbactam. Dose or gender did not affect the PK of ampicillin or sulbactam. The PK of ampicillin and sulbactam in these patients were comparable to those reported in adults. The combination was well tolerated in pediatric patients.
Effect of ascorbic acid on the resistance of the extracellular matrix to hydrolysis by tumor cells. (1980)

Extracellular matrices produced by cultured rat smooth muscle cells in the presence or absence of ascorbic acid wee used as substrates for the human fibrosarcoma cell line HT1080. The matrix elaborated by smooth muscle cells in the presence of ascorbic acid contained glycoproteins, elastin, and collagen, and all of these components were digested by the tumor cells. In contrast, the matrix elaborated in the absence of ascorbic acid which contained glycoproteins and underhydroxylated elastin but no collagen was more resistant to tumor-induced hydrolysis. The underhydroxylated elastin was particularly refractory to the tumor proteases, suggesting that the elastolytic activity produced by HT1080 cells showed a marked preference for the natural substrate containing hydroxyproline. The digestion by HT1080 cells of elastin from living cultures of smooth muscle cells was also retarded if the extracellular proteins were produced under ascorbic acid-deficient conditions. These experiments therefore do not support the notion that connective tissues made under scorbutic conditions are inherently more susceptible to tumor hydrolysis.
Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex (2017)

The innate immune response is critical for host defense and must be tightly controlled, but the molecular mechanisms responsible for its negative regulation are not yet completely understood. In this study, we report that transporter 1, ATP-binding cassette, subfamily B (TAP1), a virus-inducible endoplasmic reticulum-associated protein, negatively regulated the virus-triggered immune response. In this study, we observed upregulated expression of TAP1 following virus infection in human lung epithelial cells (A549), THP-1 monocytes, HeLa cells, and Vero cells. The overexpression of TAP1 enhanced virus replication by inhibiting the virus-triggered activation of NF-κB signaling and the production of IFNs, IFN-stimulated genes, and proinflammatory cytokines. TAP1 depletion had the opposite effect. In response to virus infection, TAP1 interacted with the TGF-β-activated kinase (TAK)1 complex and impaired the phosphorylation of TAK1, subsequently suppressing the phosphorylation of the IκB kinase complex and NF-κB inhibitor α (IκBα) as well as NF-κB nuclear translocation. Our findings collectively suggest that TAP1 plays a novel role in the negative regulation of virus-triggered NF-κB signaling and the innate immune response by targeting the TAK1 complex.
Impact of Changes of the 2020 Consensus Definitions of Invasive Aspergillosis on Clinical Trial Design: Unintended Consequences for Prevention Trials? (2021)

The updated IA consensus definitions may impact future trial designs, especially for antifungal prophylaxis studies.
Parallel hybrid electric vehicle torque distribution method (2002)

The paper deals with the supervisory control used in a Vehicle System Controller (VSC) of a Parallel Hybrid Electric Vehicle (PHEV). A description of the torque distribution algorithm that determines the prorated amount of torque demanded of each of the tractive devices to meet the driver's demand and the energy management scheme is given. The PHEV hybrid operating modes and PHEV dynamic model are presented and simulation results are shown depicting the seamless transitions from one hybrid mode to another as coordinated by this supervisory controller.
Exosomes carrying mycobacterial antigens can protect mice against Mycobacterium tuberculosis infection (2013)

Approximately 2 billion people are infected with M ycobacterium tuberculosis , the etiological agent of tuberculosis ( TB ), and an estimated 1.5 million individuals die annually from TB. Presently, M ycobacterium bovis BCG remains the only licensed TB vaccine; however, previous studies suggest its protective efficacy wanes over time and fails in preventing pulmonary TB . Therefore, a safe and effective vaccine is urgently required to replace BCG or boost BCG immunizations. Our previous studies revealed that mycobacterial proteins are released via exosomes from macrophages infected with M . tuberculosis or pulsed with M . tuberculosis culture filtrate proteins ( CFP ). In the present study, exosomes purified from macrophages treated with M . tuberculosis CFP were found to induce antigen‐specific IFN ‐γ and IL ‐2‐expressing CD 4 + and CD8 + T cells. In exosome‐vaccinated mice, there was a similar T H 1 immune response but a more limited T H 2 response compared to BCG ‐vaccinated mice. Using a low‐dose M . tuberculosis mouse aerosol infection model, exosomes from CFP ‐treated macrophages were found to both prime a protective immune response as well as boost prior BCG immunization. The protection was equal to or superior to BCG . In conclusion, our findings suggest that exosomes might serve as a novel cell‐free vaccine against an M . tuberculosis infection.
Highly Regio-, Enantio-, and Exo-selective Diels–Alder Reactions Enabled by a Bispyrrolidine Diboronate (2021)

Catalytic asymmetric Diels−Alder reaction is one of the most powerful reactions in organic chemistry. It is still a challenge to achieve high and general exo-selectivity. A novel bispyrrolidine diboronate compound has been derived from the reaction of two molecules of oxazaborolidine with one molecule of water. Upon activation, it effectively catalyzes (0.4-5 mol % loading) the Diels–Alder reaction between a variety of mono- and di-carbonyl activated dienophiles and dienes with better than 20:1 regioselectivity, up to &gt;99:1 enantioselectivity, and better than 20:1 exo/endo selectivity. Mechanistic studies show a remarkable nonlinearity in enantioselectivity, and a second-order kinetics with respect to the catalyst concentration. A model is proposed to rationalize the above observations. The reaction is expected to find wide applications in organic synthesis.
A decoupled Method for Lidar-based Close-range Relative Navigation of Tumbling Non-cooperative Target (2019)

Close-range relative navigation for non-cooperative spacecraft is the critical technique for the on-orbit servicing mission, the research on which has theoretical value and engineering significance. Based on the requirement for the autonomous relative navigation, a decoupled method of lidar-based close-range relative navigation scheme for tumbling non-cooperative target is proposed in this paper to initially estimate the position, velocity, attitude and angular velocity of the target. First, the attitude and orbit motions of target as well as the measurement from the lidar are modeled. Then, two navigation filters for rotational and translational motion of target respectively are proposed, the structure and initialization method of which are designed. The validity of the proposed relative navigation method is proved by the numerical simulation results.
Integrating XQuery and P2P in MonetDB/XQuery* (2007)

Abstract. MonetDB/XQuery * is a fully functional publicly available XML DBMS that has been extended with distributed and P2P data management functionality. Our (minimal) XQuery language extension XRPC adds the concept of RPC to XQuery, and exploits the set-at-a-time database processing model to optimize the networking cost through a technique called Bulk RPC. We describe our approach to include the services offered by diverse P2P network structures (such as DHTs), in a way that avoids any further intrusion in the XQuery language and semantics, and show how this, similarly to Bulk RPC, will lead to further query optimization opportunities where the XDBMS interacts with the underlying P2P network. We also discuss some P2P data management applications were MonetDB/XQuery* is being used (an in-home small scenario and a wide-area collaborative application). As this research is work-in-progress, we outline some research questions on our path towards defining and realizing P2P XDBMS technology. 1
Fourier analysis of videokeratography data: Clinical usefulness in grade I and subclinical keratoconus (2016)

None of the authors has a financial or proprietary interest in any material or method mentioned.
A Thematic Segmentation Procedure for Extracting Semantic Domains from Texts (1998)

International audience