gebhart/patrepeval huggingface dataset:
Cytotoxic effects of ether lipids and derivatives in human nonneoplastic bone marrow cells and leukemic cells in vitro (1987)

The effects of 2-lysophosphatidylcholine (2-LPC), the alkyl lysophospholipid derivatives (ALP) 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3) and 1-O-hexadecyl-sn-glycero-3-phospho-trimethyl-ammonio-hexanol, the 2-acetamide analog of platelet-activating factor (PAF) 1-O-octadecyl-2-acetamide-sn-glycero-3-phosphocholine, the thioether lysophospholipid derivative (TLP) BM 41.440 and the ether-linked lipoidal amine CP-46,665 on tritiated thymidine uptake and trypan blue dye exclusion were tested in vitro in various freshly explanted cell samples from human nonneoplastic bone marrow and human leukemias. In both assay systems, a dose range of 1-20 micrograms/ml of the compounds was tested after 24, 48 and 72 hr of coincubation with the cells. The trypan blue dye exclusion revealed statistically significant preferential cytotoxicity in leukemic cells for three compounds with the order of quantitative selectiveness: ET-18-OCH3 greater than BM41.440 greater than 2-acetamide analog of PAF. CP-46,665 was the most toxic compound, but did not reveal significant differences between nonneoplastic bone marrow and leukemic cells when added in concentrations greater than 1 microgram/ml. The trimethyl-ammonio-hexanol compound showed only minor activity in the majority of tests, when added at concentrations less than 20 micrograms/ml. 2-LPC was rather ineffective. The tritiated thymidine uptake showed only preferential antiproliferative effects towards leukemic cells of ET-18-OCH3 and, sometimes, within the dose time frame tested of BM 41.440. All compounds tested except 2-LPC and the trimethyl-ammonio-hexanol compound were active also in this assay (inhibition of uptake greater than 50% of the controls).(ABSTRACT TRUNCATED AT 250 WORDS)
A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers (1998)

The cellular protooncogene MYC encodes a nuclear transcription factor that is involved in regulating important cellular functions, including cell cycle progression, differentiation, and apoptosis. Dysregulated MYC expression appears critical to the development of various types of malignancies, and thus factors involved in regulating MYC expression may also play a key role in the pathogenesis of certain cancers. We have cloned one such MYC regulatory factor, termed CTCF, which is a highly evolutionarily conserved-11-zinc finger transcriptional factor possessing multiple DNA sequence specificity. CTCF binds to a number of important regulatory regions within the 5′ noncoding sequence of the human MYC oncogene, and it can regulate its transcription in several experimental systems. CTCF mRNA is expressed in cells of multiple different lineages. Enforced ectopic expression of CTCF inhibits cell growth in culture. Southern blot analyses and fluorescence in situ hybridization (FISH) with normal human metaphase chromosomes showed that the human CTCF is a single-copy gene situated at chromosome locus 16q22. Cytogenetic studies have pointed out that chromosome abnormalities (deletions) at this locus frequently occur in many different human malignancies, suggesting the presence of one or more tumor suppressor genes in the region. To narrow down their localization, several loss of heterozygosity (LOH) studies of chromosome arm 16q in sporadic breast and prostate cancers have been carried out to define the most recurrent and smallest region(s) of overlap (SRO) for commonly deleted chromosome arm 16q material. For CTCF to be considered as a candidate tumor suppressor gene associated with tumorigenesis, it should localize within one of the SROs at 16q. Fine-mapping of CTCF has enabled us to assign the CTCF gene to about a 2 centiMorgan (cM) interval of 16q22.1 between the somatic cell hybrid breakpoints CY130(D) and CY4, which is between markers D16S186 (16AC16-101) and D16S496 (AFM214zg5). This relatively small region, containing the CTCF gene, overlaps the most frequently observed SROs for common chromosomal deletions found in sporadic breast and prostate tumors. In one of four analyzed paired DNA samples from primary breast cancer patients, we have detected a tumor-specific rearrangement of CTCF exons encoding the 11-zinc-finger domain. Therefore, taken together with other CTCF properties, localization of CTCF to a narrow cancer-associated chromosome region suggests that CTCF is a novel candidate tumor suppressor gene at 16q22.1. Genes Chromosomes Cancer 22:26–36, 1998. © 1998 Wiley-Liss, Inc.
624. Organometallic fluorine compounds. Part II. The synthesis of bistrifluoromethylmercury (1949)

H. J. Emeléus and R. N. Haszeldine, J. Chem. Soc., 1949, 2953 DOI: 10.1039/JR9490002953
Struvite formation for enhanced dewaterability of digested wastewater sludge (2013)

One of the main advantages of controlled struvite formation in digested sludge is an improvement in dewaterability of the digested sludge, which eventually leads to lower volumes of dewatered sludge that need to be transported. The effects of the control parameters for struvite formation, magnesium concentration and pH, on digested sludge dewaterability were investigated and are discussed in relation to the efficiency of struvite formation. Laboratory experiments with digested activated sludge were performed in a 20 L batch reactor. CO2 was stripped from the digested sludge using a bubble aerator and magnesium chloride was added to induce struvite formation. The dewaterability of the sludge was determined by gravity filtration tests. In the experiments, either the pH or the molar magnesium to phosphate ratio (Mg:PO4) was varied. The results confirm improved sludge dewaterability after struvite formation. Magnesium to phosphate ratios above 1.0 mol/mol did not further improve dewaterability. The addition of magnesium did not prevent the need for polymer addition for sludge dewatering. An increase in pH led to a deterioration in dewaterability. The best dewaterability results were found at the lowest pH value (pH = 7.0), while stirring the sludge instead of using the bubble aerator. At these settings, an orthophosphate removal of around 80% was achieved.
Protection margins for joint operation of WiMAX 802.16e and WiMedia-defined UWB radio in personal area networks (2009)

In this paper, the radio coexistence between a WiMAX 802.16e transmission operating in the 3.5 GHz band and a WiMedia-defined UWB short-range transmission is experimentally studied in a wireless personal area network scenario. Relevant protection margins related to current UWB and WiMAX coexistence are obtained from on-the-field measurements. The coexistence has been studied evaluating the interference in both directions: First, considering an 802.16e WiMAX system victim of UWB communication and, second, considering the WiMedia-defined UWB link as a victim of the WiMAX 802.16e transmission. The experimental results indicate that a victim WiMAX (with 5 MHz bandwidth) exhibit proper operation up to 5 m transmission in presence of UWB interference if a protection margin of 2.5 m is met. WiMAX 10 MHz and 20 MHz configurations work properly in LOS links up to 10 m with UWB interference in the 2.5 m close area. In the second case, the UWB victim system exhibits proper operation at TFC1 in presence of a 5 MHz WiMAX interference if a protection distance of 5 m is met when both are in joint operation at maximum transmission power.
Reactivity of Diazoalkanes with Tantalum(V) Complexes of a Tridentate Amido-Bis(phenolate) Ligand (2009)

While a dimethyltantalum(V) complex of the [ONOcat]3− ligand ([ONOcat]H3 = N,N-bis(3,5-di-tert-butyl-2-phenol)amine) reacted with diazoalkane to afford products of insertion into the Ta−Me bond, the dichlorotantalum(V) complex [ONOcat]TaCl2(OEt2) reacted with 2 equiv of Ph2CN2 to form the ketazine adduct [ONOcat]TaCl2(η2(N,N′)-Ph2C═NN═CPh2). When Ph2CN2 was added to the dichloride in neat styrene, catalytic carbene transfer was observed to form the corresponding cyclopropane
A New Pre-Driver Design for Improving the ESD Performance of the High Voltage Tolerant I/O (2005)

The gate voltage-induced current crowding (GVICC) effect (Lee, J.H., et al., IRPS Proc., p.269-76, 2003) has been found to be the root cause of the failure of the high voltage tolerant I/O (HVT I/O) at a low-voltage ESD event. Based on this finding, a new pre-driver design is proposed to pull down the voltages of the top gate and the bottom gate of the cascode NMOS to 0 V during an ESD zapping event in order to eliminate the GVICC effect. The new pre-driver design can improve the ESD performance of the fully silicided HVT I/O from 500 V to 5 kV during HBM ESD zapping.
Movements of the Eye (1989)

R. H. S. Carpenter's second edition is a delight. As was the case with the original, it is written in an engaging style and is filled with even more insights into the "how" and "why" of the ocular motor system. The original text has been considerably expanded and revised. The book is about as up to date as a single-authored textbook can be in a field in which each year's presentations and abstracts at the Neuroscience and Association for Research in Vision and Ophthalmology meetings make the previous year's knowledge of ocular motor physiology nearly obsolete. I believe<i>Movements of the Eye</i>should be required cover-to-cover reading for ophthalmologists, neurologists, optometrists, psychologists, physiologists and others who want a detailed but readable overview of the field of eye movements and their implications for vision. The book is much more than a compendium of facts. It is suffused with Carpenter's own refreshingly
Inside Out Transobturator Vaginal Tape for the Treatment of Female Stress Urinary Incontinence: Interim Results of a Prospective Study After a 1-Year Minimum Followup (2006)

The results of this study, which suggest that the TVT-O procedure is a safe and efficient surgical treatment for female SUI, warrant further comparative evaluation of this procedure with retropubic and outside in transobturator approaches in appropriately designed, prospective, randomized trials.
A bile acid-induced apoptosis assay for colon cancer risk and associated quality control studies. (1999)

Bile acids are important in the etiology of colorectal cancer. Bile acids induce apoptosis in colonic goblet cells at concentrations comparable to those found in fecal water after high-fat meals. Preliminary evidence indicated that cells of the normal-appearing (nontumorous) portion of the colon epithelium of colon cancer patients are more resistant to bile salt-induced apoptosis than are cells from normal individuals. In the present study, 68 patients were examined, and biopsies were taken at 20 cm from the anal verge, cecum, and descending colon. The patients included 17 individuals with a history of colorectal cancer, 37 individuals with adenomas, and 14 individuals who were neoplasia free. The mean bile salt-induced apoptotic index among normal individuals was 57.6 +/- 3.47 (SE), which differed significantly (P < 0.05) from the mean value of 36.41 +/- 3.12 in individuals with a history of colon cancer. The correlation between independent observers was 0.89 (P < 0.001), indicating good interobserver reliability. Components of variance comparing interindividual versus intraindividual sources of variation suggested that site-to-site variability, both between regions of the colon and for adjacent biopsies, was larger than the interpatient variability for individuals with a history of neoplasia. Therefore, there was "patchiness" of the susceptibility of regions of the colon to bile acid-induced apoptosis in individuals with a history of neoplasia (a patchy field effect). There was no obvious correlation of low-apoptotic index regions with regions in which previous neoplasias had been found and removed. On the other hand, for normal, i.e., neoplasia-free, individuals, there was relatively less intraindividual variation compared to interindividual variation. Our assay shows an association between resistance to bile acid-induced apoptosis, measured at 20 cm from the anal verge, and colon cancer risk. Thus, this assay may prove useful as a biomarker of colon cancer risk.
Measurement of the synthesis of liver-produced plasma proteins with particular reference to dietary protein and amino acid supply (1972)

Research Article| September 01 1972 Measurement of the synthesis of liver-produced plasma proteins with particular reference to dietary protein and amino acid supply R Hoffenberg R Hoffenberg Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd © 1972 London: The Biochemical Society1972 Biochem J (1972) 129 (2): 3P. https://doi.org/10.1042/bj1290003Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation R Hoffenberg; Measurement of the synthesis of liver-produced plasma proteins with particular reference to dietary protein and amino acid supply. Biochem J 1 September 1972; 129 (2): 3P. doi: https://doi.org/10.1042/bj1290003Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1972 London: The Biochemical Society1972 Article PDF first page preview Close Modal You do not currently have access to this content.
DNA Molecular Motor Driven Micromechanical Cantilever Arrays (2005)

The unique ability of living systems to translate biochemical reactions into mechanical work has inspired the design of synthetic DNA motors which generate nanoscale motion via controlled conformational change. However, while Nature has evolved intricate mechanisms to convert molecular shape change into specific micrometer-scale mechanical cellular responses, the integration of artificial DNA motors with mechanical devices presents a major challenge. Here we report the direct integration between an ensemble of DNA motors and an array of microfabricated silicon cantilevers. The forces exerted by the precise duplex to nonclassical i-motif conformational change were probed via differential measurements using an in-situ reference cantilever coated with a nonspecific sequence of DNA. Fueled by the addition of protons, the open to close stroke of the motor induced 32 +/- 3 mN/m compressive surface stress, which corresponds to a single motor force of approximately 11 pN/m, an order of magnitude larger than previous classical hybridization studies. Furthermore, the surface-tethered conformational change was found to be highly reversible, in contrast to classical DNA motors which typically suffer rapid system poisoning. The direction and amplitude of motor-induced cantilever motion was tuneable via control of buffer pH and ionic strength, indicating that electrostatic forces play an important role in stress generation. Hybrid devices which directly harness the multiple accessible conformational states of dynamic oligonucleotides and aptamers, translating biochemical energy into micromechanical work, present a radical new approach to the construction of "smart" nanoscale machinery and mechano-biosensors.
Green Provisioning for Optical WDM Networks (2010)

Since the Internet consumes a large (and increasing) amount of energy, “green” strategies are desirable to help service providers (SP) operate their networks and provision services more energy efficiently. We focus on green provisioning strategies for optical wavelength-division multiplexing networks. A number of approaches from component layer to network layer are discussed, which should help improve the energy efficiency of the networks. Then, we consider a typical optical backbone network architecture, and minimize the operational power for provisioning. Typically, operational power depends on strategy (e.g., optical bypass versus traffic grooming), operations (e.g., electronic domain versus optical domain), and route. We analyze the constituents of operational power in various scenarios, and discuss the opportunities for energy savings. We propose a novel auxiliary graph, which can capture the power consumption of each provisioning operation. Based on the auxiliary graph, we develop a power-aware provisioning scheme to minimize the total operational power. Performance evaluation shows that our scheme always needs the least operational power, with comparison to a direct-lightpath approach and a traffic-grooming approach. The result also suggests proportional power consumption by operations (network equipment) and end-node traffic grooming to fully exploit the power-saving potential of optical networks.
The normal menstrual cycle has no effect on gastric emptying (1985)

Recent studies have demonstrated that orocaecal transit time is prolonged both in the luteal phase of the ovulatory cycle in normal women and during pregnancy, but have made no attempt to differentiate between the individual effects of gastric emptying and small intestinal transit. We have assessed the effect of the normal menstrual cycle on gastric emptying of solids and liquids in 10 women, using a dual isotope scintigraphic technique. In each subject gastric emptying was measured on 2 days: first during the follicular phase (day 8-10) and then during the luteal phase (day 18-20) of one ovulatory menstrual cycle (where day 1 was the first day of menstrual bleeding). Measures of solid and liquid gastric emptying did not change significantly between the follicular and luteal phases indicating that the normal menstrual cycle has no effect on gastric emptying.
Allowance for Spatial Dispersion of Dielectric Permittivity in Polyelectrolyte Model of DNA (1991)

In order to allow for real dielectric properties of a solvent in calculating of electrostatic characteristics of strongly charged polyions such as DNA in salt solution we consider a simple model of linear dielectric response of a medium. The interactions between charged particles are treated in the framework of self-consistent-field approximation. The basic characteristic of the problem, electrostatic potential, can be found from the solution of non-linear integro-differential equation. Specifically we consider so-called quasimacroscopic model where dielectric response of a medium depends only on the distance from the polyion. Application of the approach for calculating of the B-to-Z free energy qualitatively retains the main conclusion obtained previously within the model with fixed dielectric constant: non-monotonous behavior of the free energy differences as a function of ionic strength. At the same time, essential sensitivity of the results to specific values of dielectric parameters is observed.
Functional defects in CD4+ CD25high FoxP3+ regulatory cells in ankylosing spondylitis (2016)

Forkhead box P3 (FoxP3)-positive regulatory T cells (Tregs) play a pivotal role in the preservation of self-tolerance, and Treg dysfunction has been implicated in many autoimmune diseases. Whether and how Tregs participate in the pathogenesis of ankylosing spondylitis (AS) has not been fully elucidated. Here, we investigated Treg function and found that Tregs in peripheral blood (PB) from patients with active AS had lower FoxP3 mean fluorescence intensity (MFI) than those from healthy controls and could not fully suppress naïve T cell (Tn) proliferation. We also studied the mechanisms underlying PB Treg dysfunction in this context and found that PB Tregs failed to effectively utilize IL-2 and had relatively little STAT5 phosphorylation in active AS. Moreover, PB Tregs from patients with active AS exhibited greater CpG island methylation in the CNS2 region of the FOXP3 gene. Therefore, our findings indicate that functional defects in Tregs are present in AS. Abnormal IL-2 signalling and aberrant CNS2 epigenetic control induced functional defects in PB Tregs and represents a potential new mechanism for AS pathogenesis. These findings may aid the design of new treatment approaches for AS.
Full-wave analysis of integrated transmission lines on layered lossy media (1982)

Cohn&apos;s method of analysis of the slot line on a dielectric substrate is extended to the case of integrated lines on a lossy inhomogeneous substrate. The slow-wave characteristics of the metal-insulator-semiconductor coplanar waveguide for MMIC applications are calculated and found to be in good agreement with the experiments.
Analysis of contention tree algorithms (2000)

The Capetanakis-Tsybakov-Mikhailov (1978, 1979) contention tree algorithm provides an efficient scheme for multiaccessing a broadcast-communication channel. This paper studies the statistical properties of multiple-access contention tree algorithms with ternary feedback for an arbitrary degree of node. The particular quantities under investigation are the number of levels required for a random contender to have successful access, as well as the number of levels and the number of contention frames required to provide access for all contenders. Through classical Fourier analysis approximations to both the average and the variance are calculated as a function of the number of contenders n. It is demonstrated that in the limit of large n these quantities do not converge to a fixed mode, but contain an oscillating term as well.
Antitumor effects of regorafenib and sorafenib in preclinical models of hepatocellular carcinoma (2017)

The purpose of this study was to investigate the antitumor activity of regorafenib and sorafenib in preclinical models of HCC and to assess their mechanism of action by associated changes in protein expression in a HCC-PDX mouse model. Both drugs were administered orally once daily at 10 mg/kg (regorafenib) or 30 mg/kg (sorafenib), which recapitulate the human exposure at the maximally tolerated dose in mice. In a H129 hepatoma model, survival times differed significantly between regorafenib versus vehicle (p=0.0269; median survival times 36 vs 27 days), but not between sorafenib versus vehicle (p=0.1961; 33 vs 28 days). Effects on tumor growth were assessed in 10 patient-derived HCC xenograft (HCC-PDX) models. Significant tumor growth inhibition was observed in 8/10 models with regorafenib and 7/10 with sorafenib; in four models, superior response was observed with regorafenib versus sorafenib which was deemed not to be due to lower sorafenib exposure. Bead-based multiplex western blot analysis was performed with total protein lysates from drug- and vehicle-treated HCC-PDX xenografts. Protein expression was substantially different in regorafenib- and sorafenib-treated samples compared with vehicle. The pattern of upregulated proteins was similar with both drugs and indicates an activated RAF/MEK/ERK pathway, but more proteins were downregulated with sorafenib versus regorafenib. Overall, both regorafenib and sorafenib were effective in mouse models of HCC, although several cases showed better regorafenib activity which may explain the observed efficacy of regorafenib in sorafenib-refractory patients.