gebhart/patrepeval huggingface dataset:
Functional Nanoparticle-Based Proteomic Strategies for Characterization of Pathogenic Bacteria (2008)

Although matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) can be employed to rapidly characterize pathogenic bacteria, bacterial cultures are generally required to obtain sufficient quantities of the bacterial cells prior to MALDI MS analysis. If this time-consuming step could be eliminated, the length of time required for identification of bacterial strains would be greatly reduced. In this paper, we propose an effective means of rapidly identifying bacteria—one that does not require bacterial culturing—using functional nanoparticle-based proteomic strategies that are characterized by extremely short analysis time. In this approach, we used titania-coated magnetic iron oxide nanoparticles (Fe3O4@TiO2 NPs) as affinity probes to concentrate the target bacteria. The magnetic properties of the Fe3O4@TiO2 NPs allow the conjugated target species to be rapidly isolated from the sample solutions under a magnetic field. Taking advantage of the absorption of the magnetic Fe3O4 NPs in the microwave region of the electromagnetic spectrum, we performed the tryptic digestion of the captured bacteria under microwave heating for only 1−1.5 min prior to MALDI MS analysis. We identified the resulting biomarker ions by combining their MS/MS analysis results with protein database searches. Using this technique, we identified potential biomarker ions representing five Gram-negative bacteria: Escherichia coli O157:H7, uropathogenic E. coli, Shigella sonnei, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Finally, we demonstrated the practical feasibility of using this approach to rapidly characterize bacteria in clinical samples.
Creating Research Corpora for the Computational Study of Music: the case of the CompMusic Project (2014)

Comunicació presentada a la 53rd International Conference: Semantic audio, celebrada els dies 27 a 29 de gener de 2014 a Londres, Regne Unit.
ChemInform Abstract: OPEN‐CHAIN NITROGEN COMPOUNDS. PART III. THE FORMATION OF TRIAZENES IN THE REACTION OF DIAZONIUM SALTS WITH α‐AMINOACETONITRILE AND RELATED α‐SUBSTITUTED ALKYLAMINES (1978)

Abstract Durch Diazotierung der Aniline (Ia) und anschließende Kupplung mit Aminoacetonitril (IIIa) (als Hydrochlorid in wäßriger Lösung) werden im Eintopfverfahren die Triazene (IVa) erhalten.
A second form of infectious bursal disease virus-associated tubule contains VP4 (1997)

Preparations of density gradient-purified infectious bursal disease virus (IBDV) were found to contain full and empty icosahedral virions, type I tubules with a diameter of about 60 nm, and type II tubules 24 to 26 nm in diameter. By immunoelectron microscopy we demonstrate that virions and both types of tubular structures specifically react with anti-IBDV serum. In infected cells intracytoplasmic and intranuclear type II tubules reacted exclusively with an anti-VP4 monoclonal antibody, as did type II tubules in virion preparations. The immunofluorescence pattern with the anti-VP4 antibody correlated with electron microscopical findings. Neither purified extracellular nor intracellular virions were labeled with the anti-VP4 MAb. Our data show that the type II tubules contain VP4 and suggest that VP4 is not part of the virus particle.
The Development of Second Generation HTS Wire at American Superconductor (2007)

Development of the second generation (2G) YBCO high temperature superconducting wire has progressed rapidly and its performance is approaching, and in some areas exceeding, that of first generation (1G) HTS wire. American Superconductor's approach to the low-cost manufacturing of 2G wire is based on a wide-strip (4 cm) process using a metal organic deposition (MOD) process for the YBCO layer and the RABiTS (rolling assisted biaxially textured substrate) process for the template. In addition, the wide-strip RABiTS/MOD-YBCO process provides the flexibility to engineer practical 2G HTS wires with architectures and properties tailored for specific applications and operating conditions through slitting to custom widths and laminating with custom metallic stabilizers. This paper will review the status of the 2G manufacturing scale up at AMSC and describe the properties and architecture of the 2G wire being developed and tested for various applications including in cables, coils and fault current limiters. Performance of 100 meter class, 4 mm wide wires at 77 K, self-field has reached 100 A (250 A/cm-width) with single-coat YBCO and 140 A (350 A/cm-width) with double-coat YBCO. A 5 cm inner diameter coil fabricated from the latter wire achieved 1.5 T at 64 K, confirming the capability of the wire for coil applications.
Platelet‐rich plasma influence on human osteoblasts growth (2005)

This study concluded that PRP promotes osteoblast proliferation and suggested its clinical application to bone graft procedures in implant dentistry.
Synthesis and Antitumor Activity of Fluorocyclopentenyl-Pyrimidines (2007)

Synthesis of fluorocyclopentenyl pyrimidine nucleosides 6-9 was enantiopurely accomplished employing oxidative rearrangement, RCM reaction and electrophilic fluorination starting from d-ribose. Cytosine analog 8 was found to exhibit significant anticancer activity in various human tumor cell lines.
Evaluation of Effects in Fish Gonads (2008)

This chapter contains sections titled: Qualitative (Semiquantitative) versus Quantitative Evaluation Gonadal Staging in the Testis Gonadal Staging in the Ovary Qualitative Assessment of Histopathological Changes References
Stem cell labeling for noninvasive delivery and tracking in cardiovascular regenerative therapy (2010)

Clinical and basic scientific studies of stem cell-based therapies have shown promising results for cardiovascular diseases. Despite a rapid transition from animal studies to clinical trials, the mechanisms by which stem cells improve heart function are yet to be fully elucidated. To optimize cell therapies in patients will require a noninvasive means to evaluate cell survival, biodistribution and fate in the same subject over time. Cell labeling offers the ability to image distinct cell lineages in vivo and investigate the efficacy of these therapies using standard noninvasive imaging techniques. In this article, we will discuss the most promising cell labeling techniques for translation to clinical cardiovascular applications.
Megakaryocyte maturation involves activation of the adaptive unfolded protein response (2023)

Abstract Endoplasmic reticulum stress triggers the unfolded protein response (UPR) to promote cell survival or apoptosis. Transient endoplasmic reticulum stress activation has been reported to trigger megakaryocyte production, and UPR activation has been reported as a feature of megakaryocytic cancers. However, the role of UPR signaling in megakaryocyte biology is not fully understood. We studied the involvement of UPR in human megakaryocytic differentiation using PMA (phorbol 12-myristate 13-acetate)-induced maturation of megakaryoblastic cell lines and thrombopoietin-induced differentiation of human peripheral blood-derived progenitors. Our results demonstrate that an adaptive UPR is a feature of megakaryocytic differentiation, and that this response is not associated with ER stress-induced apoptosis. Differentiation did not alter the response to the canonical endoplasmic reticulum stressors DTT or thapsigargin. However, thapsigargin, but not DTT, inhibited differentiation, consistent with the involvement of Ca 2+ signaling in megakaryocyte differentiation.
Problems and Opportunities in Regulation of Benzodiazepines (1998)

A commentary on current national and international regulation of benzodiazepine-like drugs is presented. The policies and decision making associated with these regulations also are discussed, both in terms of historical mistakes and current problems. Finally, a proposal is made to improve the methods by which regulations are put into effect.
Charge carrier migration and hole extraction from MAPbI3 (2019)

Abstract Linear as well as time resolved absorption measurements were performed on 40 nm and 170 nm thick MAPbL films with PEDOT:PSS hole extraction layer, spin-coated on quartz substrate. From linear absorption measurements exciton binding energy of 18 – 19 meV and band gap of 1.60 - 1.62 eV was deduced. Transient absorption spectra after the excitation at 1.77 eV showed a strong difference in carrier recombination dynamics for the two MAPbI 3 films of different thicknesses. From the analysis on the decay dynamics, hole population lifetime of 0.3 ns and 3.5 ns for 40 nm and 170 nm films, respectively, are determined. A numerical 1D diffusion model was used to model the carrier relaxation dynamics yielding hole diffusion constants of 0.025 - 0.030 cm 2 s −1 , which results in a hole mobility of 1 cm 2 (Vs) −1 in these MAPbI 3 films.
CD40 regulation of death domains containing receptors and their ligands on lymphoma B cells (1998)

Within the tumour necrosis factor (TNF) family the induction of apoptosis is restricted to some ligand-receptors pairs, including TNF-TNF receptor type I (TNFRI/p55), FasL-Fas, TNF-related apoptosis-inducing ligand (TRAIL) and its death-receptors (DR)-4 and -5. The pair CD40L-CD40 belongs to the same family but rescues B cells from apoptosis. To investigate how these opposing actions are cross-linked, purified follicular lymphoma (FL) cells were activated upon a human CD40L-transfected murine fibroblastic layer, then RNA messengers for the above molecules were analysed using RT-PCR. The observed down-modulation of TRAIL and up-regulation of TNF and Fas transcripts might account for CD40-CD40L-mediated FL cell survival.
Synthesis, Three‐Dimensional Structure, and Specific 15N‐Labelling of the Streptococcal Protein G B1‐Domain (1995)

The 55-amino-acid B1-domain of the streptococcal protein G shows a high binding affinity to IgG isolated from a wide range of mammalian species. Since the B1-domain forms an extremely stable globular folding unit containing the major secondary structure elements and is devoid of proline residues and disulfide bridges, it is also a useful tool for protein folding and stability studies. Its small size makes this protein an ideal candidate for production by chemical synthesis, allowing incorporation of non-natural amino acids with the possibility of assessing the influence of such residues on both the functional and structural characteristics of proteins. In this study, we employed three successive chemical syntheses of the B1-domain in order to define the optimal conditions of coupling and protection. The stepwise solid-phase methodology using the tertbutyloxycarbonyl/benzyl strategy was used for this purpose. First, the sequence assembly difficulties were evaluated. After analyzing of the problems found during assembly, a second optimized synthesis was performed leading to formation of a synthetic B1-domain with a higher yield; the synthetic B1-domain was completely functional in its binding properties to IgG. Three orthogonal purification steps (gel-permeation, reverse-phase and ion-exchange HPLC) were required to obtain a sample suitable for structural analysis by high-resolution NMR. This study led to the conclusion that the synthetic B1-domain adopts a three-dimensional structure identical to that of the molecule obtained by recombinant techniques [Gronenborn, A.M., Filpula, D. R., Essig, N. Z., Achari, A., Whitlow, M., Wingfield, P. T. & Clore, G. M. (1991) Science 253, 657-661]. To demonstrate the usefulness of the chemical approach for the specific introduction of labelled amino acids in the primary structure, fourteen alpha-15N-labelled amino acids were incorporated at selected critical positions during the third synthesis. This analog is the first in a series of molecules planned to study in detail the folding dynamics of the B1-domain.
Segmental relaxation and molecular structure in polybutadienes and polyisoprene. [Erratum to document cited in CA115(14):137101w] (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleSegmental relaxation and molecular structure in polybutadienes and polyisoprene. [Erratum to document cited in CA115(14):137101w]C. M. Roland and K. L. NgaiCite this: Macromolecules 1992, 25, 6, 1844Publication Date (Print):March 1, 1992Publication History Published online1 May 2002Published inissue 1 March 1992https://pubs.acs.org/doi/10.1021/ma00032a038https://doi.org/10.1021/ma00032a038research-articleACS PublicationsRequest reuse permissionsArticle Views68Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Excess Noise in Quartz Crystal Resonators (1983)

Abstract : Frequency and phase noise in quartz crystal resonators are studied as a function of the driving power. At low power, where the crystal behaves linearly, 1/f fluctuations of the resonance frequency are observed. At medium power the nonlinearities of the crystal significantly increase the phase fluctuations at low Fourier frequencies. At high power, thermal instabilities and chaotic behavior occur characterized by the generation of high level white noise.
Molecular and Cellular Insights into the Development of Uterine Fibroids (2021)

Uterine leiomyomas represent the most common benign gynecologic tumor. These hormone-dependent smooth-muscle formations occur with an estimated prevalence of ~70% among women of reproductive age and cause symptoms including pain, abnormal uterine bleeding, infertility, and recurrent abortion. Despite the prevalence and public health impact of uterine leiomyomas, available treatments remain limited. Among the potential causes of leiomyomas, early hormonal exposure during periods of development may result in developmental reprogramming via epigenetic changes that persist in adulthood, leading to disease onset or progression. Recent developments in unbiased high-throughput sequencing technology enable powerful approaches to detect driver mutations, yielding new insights into the genomic instability of leiomyomas. Current data also suggest that each leiomyoma originates from the clonal expansion of a single transformed somatic stem cell of the myometrium. In this review, we propose an integrated cellular and molecular view of the origins of leiomyomas, as well as paradigm-shifting studies that will lead to better understanding and the future development of non-surgical treatments for these highly frequent tumors.
Screening the human exome: a comparison of whole genome and whole transcriptome sequencing (2010)

We conclude that as long as a tissue relevant to the trait under study is available and suitable quality control screens are implemented, RNA-Seq is a fast and inexpensive alternative approach for finding coding variants in genes with sufficiently high expression levels.
MP3 for Dummies? [With CDROM] (1999)

From the Publisher:
MP3 has created a sensation both online and in the music world, generating waves of interest from Internet users and notice from the recording industry. MP3 for Dummies sorts through the hype surrounding the hottest technology for sharing music over the Web and shows exactly how to use it. This guide shows users how to download and use PC-based MP3 players, find MP3 files online, make MP3 files from home recordings - and understand what's legal and what's not. CD-ROM contains MP3 tools and sample files that can be heard on a desktop PC.
Highly Efficient Synthesis and Imaging Studies of an Arylpiperazine Derivative as a 5-HT1A Receptor Imaging Agent (2006)

Abstract A novel and straightforward microwave synthesis of a new arylpiperazine derivative 3 which has an N2S2 moiety has been developed and radiolabeled with an optimum radionuclide of technetium in the presence of a borohydride exchange resin (BER) as a reducing agent. According to the present study this new radiolabeled compound 4 could have the potential for a diagnostic application as an imaging agent of a serotonin neuroreceptor.
New Metal-Free One-Pot Synthesis of Substituted Allenes from Enones (2008)

A new metal-free, one-pot synthesis of substituted allenes from enones was discovered for the first time, in which a tertiary amine as a base was found to be an effective promoter in such novel transformations. The present synthetic protocol proceeded readily with high compatibility of sensitive functional groups, and it provides a new efficient way to access a series of synthetically important allenes without the use of metallic reagents or catalysts.
Post-Pleistocene radiation of the pea aphid complex revealed by rapidly evolving endosymbionts (2009)

Adaptation to different resources has the potential to cause rapid species diversification, but few studies have been able to quantify the time scale of recent adaptive radiations. The pea aphid, Acyrthosiphon pisum, a model of speciation for host-specialized parasites, consists of several biotypes (races or species) living on distinct legume hosts. To document this radiation, we used rapidly evolving sequences from Buchnera, the maternally transmitted bacterial endosymbiont of aphids. Analyses of Buchnera pseudogene sequences revealed that 11 host-associated biotypes sort mostly into distinct matrilines despite low sequence divergence. A calibration based on divergence times of 7 sequenced genomes of Buchnera allowed us to date the last maternal ancestor of these biotypes between 8,000 and 16,000 years, with a burst of diversification at an estimated 3,600-9,500 years. The recency of this diversification, which is supported by microsatellite data, implies that the pea aphid complex ranks among the most rapid adaptive radiations yet documented. This diversification coincides with post-Pleistocene warming and with the domestication and anthropogenic range expansion of several of the legume hosts of pea aphids. Thus, we hypothesize that the new availability or abundance of resources triggered a cascade of divergence events in this newly formed complex.
Registration method between ToF and color cameras for face recognition (2011)

Since 3D data has additional depth information than 2D image, it can be used various researches and applications such as robot vision, car navigation and human computer interaction. Especially, 3D face recognition is robust against facial pose and light variation since it can use 3D face shape information such as facial profile, curvature and depth map. However, it is difficult to acquire 3D face data in real-time since conventional 3D data acquisition systems like laser scanner or structured light system are very slow for the data capturing. Recently, Time-of-Flight (ToF) camera provides full range distance data at high framerates and thus comes into the spotlight as an alternative to conventional 3D acquisition systems. ToF camera provides both distance images and gray-scale images simultaneously in realtime. However, it has drawbacks which are low resolution and gray-scale image, not color image. In this paper, a registration method of color and ToF cameras is presented to find precise corresponding color pixel information with range distance data from ToF camera.
Recent Advances in Nanoscale Bioinspired Materials (2014)

Natural materials have been a fundamental part of human life since the dawn of civilization. However, due to exploitation of natural resources and cost issues, synthetic materials replaced bio-derived materials in the last century. Recent advances in bio- and nano-technologies pave the way for developing eco-friendly materials that could be produced easily from renewable resources at reduced cost and in a broad array of useful applications. This feature article highlights structural and functional characteristics of bio-derived materials, which will expedite the design fabrication and synthesis of eco-friendly and recyclable advanced nano-materials and devices.
Homogeneous quantum cascade lasers operating as terahertz frequency combs over their entire operational regime (2021)

We report a homogeneous quantum cascade laser (QCL) emitting at terahertz (THz) frequencies, with a total spectral emission of about 0.6 THz, centered around 3.3 THz, a current density dynamic range Jdr = 1.53, and a continuous wave output power of 7mW. The analysis of the intermode beatnote unveils that the devised laser operates as an optical frequency comb (FC) synthesizer over the whole laser operational regime, with up to 36 optically active laser modes delivering ∼200 μW of optical power per opticalmode, a power level unreached so far in any THz QCL FC. A stable and narrow single beatnote, reaching a minimum linewidth of about 500 Hz, is observed over a current density range of 240 A/cm2 and even across the negative differential resistance region. We further prove that the QCL FC can be injection locked with moderate radio frequency power at the intermode beatnote frequency, covering a locking range of 1.2 MHz. The demonstration of stable FC operation, in a QCL, over the full current density dynamic range, and without any external dispersion compensation mechanism, makes our proposed homogenous THz QCL an ideal tool for metrological applications requiring mode-hop electrical tunability and a tight control of the frequency and phase jitter.
Near-Optimal Codebook Constructions for Limited Feedback Beamforming in Correlated MIMO Channels with Few Antennas (2006)

Transmit beamforming with receive combining is a low-complexity solution that achieves the full diversity afforded by a multi-antenna channel. Building on our recent result which shows that even channel statistics are sufficient to achieve perfect feedback performance (in the limit of antenna dimensions) with beamforming and combining, we propose near-optimal codebook designs for correlated channels with a focus on few antennas at the transmitter and the receiver. In the process, we refine the answer to the question: When are channel statistics sufficient to achieve near perfect feedback performance? We show that the condition number of the transmit and receive covariance matrices hold the key to this question. We partition the transmit and receive covariance spaces into 4 regions based on well and ill-conditioning of the covariance matrices and show that the number of bits required for near perfect feedback performance is dependent on the condition numbers of these matrices
A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types (2016)

There is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since genes are often expressed in many cell types, many of these lines have relatively broad expression patterns. We report an alternative transgenic approach capturing distal enhancers for more focused expression. We identified an enhancer trap probe often producing restricted reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon. We established more than 200 lines and found many lines that label small subsets of neurons in brain substructures, including known and novel cell types. Images and other information about each line are available online (enhancertrap.bio.brandeis.edu).