gebhart/patrepeval huggingface dataset:
Urokinase receptor expression on human microvascular endothelial cells is increased by hypoxia: implications for capillary-like tube formation in a fibrin matrix (2000)

Hypoxia stimulates angiogenesis, the formation of new blood vessels. This study evaluates the direct effect of hypoxia (1% oxygen) on the angiogenic response of human microvascular endothelial cells (hMVECs) seeded on top of a 3-dimensional fibrin matrix. hMVECs stimulated with fibroblast growth factor-2 (FGF-2) or vascular endothelial growth factor (VEGF) together with tumor necrosis factor-alpha (TNF-alpha) formed 2- to 3-fold more tubular structures under hypoxic conditions than in normoxic (20% oxygen) conditions. In both conditions the in-growth of capillary-like tubular structures into fibrin required cell-bound urokinase-type plasminogen activator (uPA) and plasmin activities. The hypoxia-induced increase in tube formation was accompanied by a decrease in uPA accumulation in the conditioned medium. This decrease in uPA level was completely abolished by uPA receptor-blocking antibodies. During hypoxic culturing uPA receptor activity and messenger RNA (mRNA) were indeed increased. This increase and, as a consequence, an increase in plasmin formation contribute to the hypoxia-induced stimulation of tube formation. A possible contribution of VEGF-A to the increased formation under hypoxic conditions is unlikely because there was no increased VEGF-A expression detected under hypoxic conditions, and the hypoxia-induced tube formation by FGF-2 and TNF-alpha was not inhibited by soluble VEGFR-1 (sVEGFR-1), or by antibodies blocking VEGFR-2. Furthermore, although the alpha(v)-integrin subunit was enhanced by hypoxia, blocking antibodies against alpha(v)beta(3)- and alpha(v)beta(5)-integrins had no effect on hypoxia-induced tube formation. Hypoxia increases uPA association and the angiogenic response of human endothelial cells in a fibrin matrix; the increase in the uPA receptor is an important determinant in this process. (Blood. 2000;96:2775-2783)
Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens (2015)

Standardization of a Multiplex Magnetic Bead-based for Simultaneous Detection of IgG to Plasmodium Antigens Multiplex assays are currently used to facilitate the evaluation of antibody (Ab) responses to multiple Plasmodium falciparum antigens from large field-based epidemiological studies. The present study aimed at (i) optimizing parameters of a novel cost-effective, compact and reliable magnetic bead-based multiplex immunoassay (MBA) carried out with the MAGPIX®-Luminex system and (ii) comparing the results with those obtained using standard ELISA technology.
Reducing energy dissipation of video memory by adaptive bit-width compression (2003)

A new architectural technique to reduce the energy dissipation of video memory is proposed. Unlike existing approaches, the technique exploits the pixel correlation in video sequences, dynamically adjusting the memory bit-width to the number of bits changed per pixel. Instead of treating the data bits independently, we group the most significant bits together, activating the corresponding group of bit-lines adaptively to data variation. The method is not restricted to the specific bit-patterns nor depends on the storage phase. It works equally well on read and write accesses, as well as during precharging. Simulation results show that using this method we can reduce the total energy consumption of frame memory by 20% without affecting the picture quality.
Issues in automating the extraction of a system model from CAD databases for use in model-based reasoning (1991)

The knowledge acquisition task has proven to be one of the greatest obstacles to the widespread use of knowledge-based systems. Traditional methods for eliciting knowledge from the various sources (usually human experts) are generally labor-intensive. This has made development of such systems quite expensive in many cases. One attractive alternative for breaking the bottleneck is to automate the knowledge acquisition task.
Comparative Analysis of Forest Conservation and Sustainable Forest Management in China and Germany (2013)

Forestry development in Germany and China does not seem to be comparable due to differences in nature, politics, economy, society, culture and demographic situation, but the comparative study on these still has great important theoretical and practical significance. Taking into account the significant differences between the two countries, a comparative analysis was carried out through following aspects from (1) forestry policies, strategies and programs, (2) legal regulatory, administrative and socio-economic framework conditions, (3) tools and provisions and other aspects of bilateral forest protection and sustainable forest management. Researches on forest protection and sustainable forest management framework were carried out by combination of forestry development and historical review discussion in order to learn from the German experiences to promote the development of China's forestry.
Analogues of Cytostatic, Fused Indazolinones: Synthesis, Conformational Analysis and Cytostatic Activity Against HeLa Cells of Some 1‐Substituted Indazolols, 2‐Substituted Indazolinones, and Related Compounds (1996)

Abstract A number of indazol‐3‐ol and indazolin‐3‐one derivatives were designed as open‐chain analogues of some previously studied cytostatic tetranuclear indazolinones. The former were prepared by starting with alkylation of the parent indazolinones ( 11 or its 5‐H analogue). Special mention deserves the method we developed for the direct alkylation of position 2 of indazolinone 11 with benzyl or picolyl halides. The prepared compounds were evaluated as cytostatic agents against HeLa cells, but only 1‐benzylindazolol 5a and 1‐(2‐fluorobenzyl)‐3‐methoxy‐ and 1‐benzyl‐3‐methoxyindazoles 14h , i display significant activity. Additional 13 C‐NMR studies and conformational analysis using molecular mechanics and quantum‐mechanical semiempirical techniques confirm that the compounds designed as analogues of the model cytostatic indazolinones are not able to mimic effectively the structural features of the latter.
Remote Class Loading for Mobile Devices (2007)

The CLDC defines the base feature set of a Java Virtual Machine with limited application programming interfaces that aims to be deployed on the most restricted subset of hardware. This paper envisages the design of a platform that will allow remote class loading on mobile devices. Mobile networks are made up of mobile devices without underlying infrastructure, but their features make them intrinsically difficult to manage. Therefore a flexible mechanism is designed based on remote class loading. The proposed virtual machine, MODECO, allows remote class loading in contrast to the CLDC machine that has not such a feature. MODECO is evaluated in relation to the CLDC reference implementation. It is proved that the changes in the class loading mechanism have a small influence in the overall performance of the virtual machine.
Semiconductor ultraviolet detectors (1996)

This paper presents an overview of semiconductor ultraviolet (UV) detectors that are currently available and associated technologies that are undergoing further development. At the beginning, the classification of UV detectors and general requirements imposed on these detectors are presented. Further consideration are restricted to modern semiconductor UV detectors, so the current state-of-the-art of different types of semiconductor UV detectors is presented. Hitherto, the semiconductor UV detectors have been mainly fabricated using Si. Industries such as the aerospace, automotive, petroleum, and others have continuously provided the impetus pushing the development of fringe technologies which are tolerant of increasingly high temperatures and hostile environments. As a result, the main effort are currently directed to a new generation of UV detectors fabricated from wide-band-gap semiconductors between them the most promising are diamond and AlGaN. The latest progress in development of AlGaN UV detectors is finally described in detail.
SOLAR ABSORPTION IN THICK AND MULTILAYERED GLAZINGS (2002)

Thick and multilayered glazings generally have a nonuniform distribution of absorbed solar radiation which is not taken into account by current methods for calculating the center of glass solar gain and thermal performance of glazing systems. This paper presents a more accurate method for calculating the distribution of absorbed solar radiation inside thick and multilayered glazings and demonstrates that this can result in a small but significant difference in steady-state temperature profile and Solar Heat Gain Coefficient for some types of glazing systems when compared to the results of current methods. This indicates that a more detailed approach to calculating the distribution of absorbed solar radiation inside glazings and resulting thermal performance may be justified for certain applications.
The PH Domain and the Polybasic c Domain of Cytohesin-1 Cooperate specifically in Plasma Membrane Association and Cellular Function (1998)

Recruitment of intracellular proteins to the plasma membrane is a commonly found requirement for the initiation of signal transduction events. The recently discovered pleckstrin homology (PH) domain, a structurally conserved element found in approximately 100 signaling proteins, has been implicated in this function, because some PH domains have been described to be involved in plasma membrane association. Furthermore, several PH domains bind to the phosphoinositides phosphatidylinositol-(4,5)-bisphosphate and phosphatidylinositol-(3,4,5)-trisphosphate in vitro, however, mostly with low affinity. It is unclear how such weak interactions can be responsible for observed membrane binding in vivo as well as the resulting biological phenomena. Here, we investigate the structural and functional requirements for membrane association of cytohesin-1, a recently discovered regulatory protein of T cell adhesion. We demonstrate that both the PH domain and the adjacent carboxyl-terminal polybasic sequence of cytohesin-1 (c domain) are necessary for plasma membrane association and biological function, namely interference with Jurkat cell adhesion to intercellular adhesion molecule 1. Biosensor measurements revealed that phosphatidylinositol-(3,4,5)-trisphosphate binds to the PH domain and c domain together with high affinity (100 nM), whereas the isolated PH domain has a substantially lower affinity (2-3 microM). The cooperativity of both elements appears specific, because a chimeric protein, consisting of the c domain of cytohesin-1 and the PH domain of the beta-adrenergic receptor kinase does not associate with membranes, nor does it inhibit adhesion. Moreover, replacement of the c domain of cytohesin-1 with a palmitoylation-isoprenylation motif partially restored the biological function, but the specific targeting to the plasma membrane was not retained. Thus we conclude that two elements of cytohesin-1, the PH domain and the c domain, are required and sufficient for membrane association. This appears to be a common mechanism for plasma membrane targeting of PH domains, because we observed a similar functional cooperativity of the PH domain of Bruton's tyrosine kinase with the adjacent Bruton's tyrosine kinase motif, a novel zinc-containing fold.
Emergence of Electronic Home Monitoring in Chronic Heart Failure: Rationale, Feasibility, and Early Results With the HomMed Sentry™‐Observer™ System (2000)

Electronic home monitoring for chronic heart failure is emerging as an available option to add to our armamentarium as a vital part of the multidisciplinary care process. This investigation describes the early clinical results of a multicenter study that suggests that important trends in medical resource utilization may be attained by the use of this modality. (c)2000 by CHF, Inc.
Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae (2015)

Caveolae are invaginations of the cell surface thought to regulate membrane tension, signalling, adhesion and lipid homeostasis owing to their dynamic behaviour ranging from stable surface association to dynamic rounds of fission and fusion with the plasma membrane. The caveolae coat is generated by oligomerisation of the membrane protein caveolin and the family of cavin proteins. Here, we show that cavin3 (also known as PRKCDBP) is targeted to caveolae by cavin1 (also known as PTRF) where it interacts with the scaffolding domain of caveolin1 and promote caveolae dynamics. We found that the N-terminal region of cavin3 binds a trimer of the cavin1 N-terminus in competition with a homologous cavin2 (also known as SDPR) region, showing that the cavins form distinct subcomplexes through their N-terminal regions. Our data shows that cavin3 is enriched at deeply invaginated caveolae and that loss of cavin3 in cells results in an increase of stable caveolae and a decrease of caveolae that are only present at the membrane for a short time. We propose that cavin3 is recruited to the caveolae coat by cavin1 to interact with caveolin1 and regulate the duration time of caveolae at the plasma membrane.
ChemInform Abstract: A New Versatile Synthesis of 4‐Nitroindoles. (1984)

Abstract Die 3‐Nitro‐o‐toluidide (I) werden mit Oxalsäurediethylester (II) über die Stufe der Ketoester (III) zu den 4‐Nitroindolen cyclisiert.
ChemInform Abstract: Methyl 2‐Benzoylamino‐3‐dimethylaminopropenoate in the Synthesis of Heterocyclic Systems (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Monoclonal Antibodies to Rabbit Brain Acetylcholinesterase: Selective Enzyme Inhibition, Differential Affinity for Enzyme Forms, and Cross‐Reactivity with Other Mammalian Cholinesterases (1985)

Eleven unique monoclonal IgG antibodies were raised against rabbit brain acetylcholinesterase (AChE, EC 3.1.1.7), purified to electrophoretic homogeneity by a two-step procedure involving immunoaffinity chromatography. The apparent dissociation constants of these antibodies for rabbit AChE ranged from about 10 nM to more than 100 nM (assuming one binding site per catalytic subunit). Species cross-reactivity was investigated with crude brain extracts from rabbit, rat, mouse cat, guinea pig, and human. One antibody bound rabbit AChE exclusively; most bound AChE from three or four species; two bound enzyme from all species tested. Identical, moderate affinity for rat and mouse brain AChE was displayed by two antibodies; two others were able to distinguish between these similar antigens. Nine of the antibodies had lowered affinity for AChE in the presence of 1 M NaCl, but two were salt resistant. Analysis of mutual interferences in AChE binding suggested that certain of the antibodies were competing for nearby epitopes on the AChE surface. One antibody was a potent AChE inhibitor (IC50 = 10(-8) M), blocking up to 90% of the enzyme activity. Most of the antibodies were less able to bind the readily soluble AChE of detergent-free brain extracts than the AChE which required detergent for solubilization. The extreme case, an antibody that was unable to recognize nearly half of the "soluble" AChE, was suspected of lacking affinity for the hydrophilic enzyme form.
Drug metabolic enzyme genotype-phenotype discrepancy: High phenoconversion rate in patients treated with antidepressants (2022)

Cytochromes from the P450 family (CYP) play a central role in the primary metabolism of frequently prescribed antidepressants, potentially affecting their efficacy and tolerance. There are however important differences in the drug metabolic capacities of each individual resulting from a combination of intrinsic and environmental factors. This variability can present an important risk for patients and increases the difficulty of drug prescription in clinical practice. Pharmacogenetic studies have uncovered a number of alleles defining the intrinsic metabolizer status, however, additional factors affecting cytochrome activity can modify this activity and result in a phenoconversion. The present study investigates the discrepancy between the genetically predicted and actually measured activities for the six most important liver cytochromes (CYP1A2, CYP2B6, CYP2C9, CYP2C19, CYP2D6 and CYP3A4) in a cohort of patients under antidepressant treatment, previously shown to have a high proportion of patients with low metabolizing activities. We now performed the genetic characterization of this cohort to determine the extent of the genetic versus environmental contribution in these decreased activities. For all enzyme tested, we observed an important rate of phenoconversion, affecting between 33 % and 65 % of the patients, as well as a significant (p < 1E-06) global reduction in the effective but not predicted activities of CYP2D6, CYP2C9 and CYP2C19 compared to the general population. Our results highlight the advantages of phenotyping versus genotyping as well as the increased risk of treatment failure or adverse effect occurrence in a polymedicated population.
Reducing the impurity incorporation from residual gas by ion bombardment during high vacuum magnetron sputtering (2006)

The influence of ion energy on the hydrogen incorporation has been investigated for alumina thin films, deposited by reactive magnetron sputtering in an Ar∕O2∕H2O environment. Ar+ with an average kinetic energy of ∼5eV was determined to be the dominating species in the plasma. The films were analyzed with x-ray diffraction, x-ray photoelectron spectroscopy, and elastic recoil detection analysis, demonstrating evidence for amorphous films with stoichiometric O∕Al ratio. As the substrate bias potential was increased from −15V (floating potential) to −100V, the hydrogen content decreased by ∼70%, from 9.1to2.8at.%. Based on ab initio calculations, these results may be understood by thermodynamic principles, where a supply of energy enables surface diffusion, H2 formation, and desorption [Rosén et al., J. Phys.: Condens. Matter 17, L137 (2005)]. These findings are of importance for the understanding of the correlation between ion energy and film composition and also show a pathway to reduce impurity incorporation during film growth in a high vacuum ambient.
Phytochemical and Ecological Analysis of Two Varieties of Hemp (Cannabis sativa L.) Grown in a Mountain Environment of Italian Alps (2019)

Hemp (<i>Cannabis sativa</i> L.) is a multifunctional crop that is capable of prompt environmental adaptation. In this study, a monoecious cultivar (Futura 75) and a dioecious one (Finola) were tested in a mountain area in Valsaviore (Rhaetian Alps, Italy; elevation: 1,100 m a.s.l.) during the growing season 2018. Phytochemical behavior was evaluated by different analytical approaches: HPLC-high-resolution mass spectrometry, SDS-PAGE LC-MS/MS, HS-SPME GC-MS, and GC-FID in order to obtain complete profile of two varieties cultivated in altitude. CSR functional strategy used for ecological evaluation revealed that both genotypes are mainly competitors, although Finola is more stress tolerator (C:S:R = 57:26:17%) than Futura (C:S:R = 69:15:16%). The Finola inflorescences were characterized by higher quantities of β-ocimene and α-terpinolene, while α- and ß-pinene accompanied by extremely high ß-myrcene were found as predominant in Futura. Both varieties were rich in sesquiterpenes (45 recognized) among which <i>trans</i>-caryophyllene and α-humulene were the most abundant. Total tetrahydrocannabinol level was lower than 0.1%, while the most abundant cannabinoid was cannabidiolic acid (CBDA): 2.3% found in Finola vs. 2.7% revealed for Futura. The level of corresponding neutral form, cannabidiol, varied drastically: 0.27% (Finola) vs. 0.056% (Futura). Finola showed the unique cannabinoid profile with unexpectedly high cannabidivarin, 2-fold higher that corresponding acidic analogue, whereas the particularity of Futura 75 was the occurrence of cannabigerolic acid (CBGA) in the quantities that was double than those exposed for Finola. The seeds from both chemovars proved to be rich in polyunsaturated fatty acids, and Finola showed a higher ratio ω6/ω3. No difference was found in the protein content, and the SDS-PAGE profile was similar. The most abundant protein was edestin, followed by heat shock protein 70, ß-conglycinin, and vicilin. In conclusion, comprehensive phytochemical and ecological study of two fiber-type varieties cultivated in Italian Alps displayed specific, legal, and safe cannabinoids profile, followed by particular terpene composition, polyunsaturated fatty acids content, and favorable protein profile. This postulates that geographical provenience of hemp should be considered in selecting a variety that would be suitable for a specific end-use nutraceutical application.
Full duplex MIMO radios (2014)

This paper presents the design and implementation of the first in-band full duplex WiFi-PHY based MIMO radios that practically achieve the theoretical doubling of throughput. Our design solves two fundamental challenges associated with MIMO full duplex: complexity and performance. Our design achieves full duplex with a cancellation design whose complexity scales almost linearly with the number of antennas, this complexity is close to the optimal possible. Further we also design novel digital estimation and cancellation algorithms that eliminate almost all interference and achieves the same performance as a single antenna full duplex SISO system, which is again the best possible performance. We prototype our design by building our own analog circuit boards and integrating them with a WiFi-PHY compatible standard WARP software radio implementation. We show experimentally that our design works robustly in noisy indoor environments, and provides close to the expected theoretical doubling of throughput in practice.
A noise-based IC random number generator for applications in cryptography (2000)

The design of a mixed-signal random number generator (RNG) integrated circuit (IC) suitable for integration with hardware cryptographic systems is presented. Certain applications in cryptography require the use of a truly RNG, a device which produces unpredictable and unbiased digital signals derived from a fundamental noise mechanism. For IC-based cryptographic systems, an RNG must harness randomness from a low-power noise signal yet remain insensitive to deterministic influences such as crosstalk, power supply noise, and clock signal coupling through the substrate. An RNG IC utilizing established analog IC design techniques was designed and fabricated in a 2-/spl mu/m CMOS technology. Sequences generated by the experimental system repeatedly passed many standard randomness tests for bit rates up to 1.4 MHz. No changes in randomness performance were observed as the system was exposed to power supply noise and substrate signal coupling. The system occupies a total chip area of 1.5 mm/sup 2/ and dissipates 3.9 mW of power.
Catalisadores de platina suportada em materiais de carbono com diferentes propriedades de adsorção (2004)

1 Laboratorio de Catalise e Materiais/Departamento de Engenharia Quimica, Faculdade de Engenharia da Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto 2 Departamento de Tecnologia Quimica, Escola Superior de Tecnologia e de Gestao do Instituto Politecnico de Braganca, Campus de Santa Apolonia, 5300-857 Braganca 3 Laboratoire de Catalyse, Chimie Fine et Polymeres-Ecole Nationale Superieure d’Ingenieurs en Arts Chimiques et Technologiques, 118 route de Narbonne, 31077 Toulouse Cedex 4, France htgomes@ipb.pt