gebhart/patrepeval huggingface dataset:
Staining of Glycoproteins/Proteoglycans on SDS-Gels (1996)

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of mol-wt determinations. In this chapter, staining methods will be described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.
Systematic review of quality of life and functional outcomes in randomized placebo-controlled studies of medications for attention-deficit/hyperactivity disorder (2017)

Children, adolescents and adults with attention-deficit/hyperactivity disorder (ADHD) experience functional impairment and poor health-related quality of life (HRQoL) in addition to symptoms of inattention/hyperactivity-impulsivity. To synthesize qualitatively the published evidence from randomized, double-blind, placebo-controlled trials of the effectiveness of pharmacotherapy on functional impairment or HRQoL in patients with ADHD, a systematic PubMed searching and screening strategy was designed to identify journal articles meeting pre-specified criteria. Post hoc analyses and meta-analyses were excluded. HRQoL outcomes, functional outcomes and the principal ADHD symptom-based outcome were extracted from included studies. An effect size of 0.5 versus placebo was used as a threshold for potential clinical relevance (unreported effect sizes were calculated when possible). Of 291 records screened, 35 articles describing 34 studies were included. HRQoL/functioning was usually self-rated in adults and proxy-rated in children/adolescents. Baseline data indicated substantial HRQoL deficits in children/adolescents. Placebo-adjusted effects of medication on ADHD symptoms, HRQoL and functioning, respectively, were statistically or nominally significant in 18/18, 10/12 and 7/9 studies in children/adolescents and 14/16, 9/11 and 9/10 studies in adults. Effect sizes were ≥0.5 versus placebo for symptoms, HRQoL and functioning, respectively, in 14/16, 7/9 and 4/8 studies in children/adolescents; and 6/12, 1/6 and 1/8 studies in adults. Effect sizes were typically larger for stimulants than for non-stimulants, for symptoms than for HRQoL/functioning, and for children/adolescents than for adults. The efficacy of ADHD medication extends beyond symptom control and may help reduce the related but distinct functional impairments and HRQoL deficits in patients with ADHD.
Determining user specific spectrum usage via sparse channel characteristics (2015)

We consider a device-to-device scenario, where multiple users transmit complex symbols with constant amplitude on exclusive OFDM resources. However, the spectrum usage and therefore the assignment of resources by each user is unknown at the receiver. The number of resources allocated by each user are well chosen, so that the sparse channel characteristics can be coarsely estimated once the correct resource assignment is known. The receiver faces an inverse problem with two unknowns, the resource allocation and the spectral channel power. We propose an objective function, which identifies the correct resource assignment by just observing the received signal and exploiting the sparse channel properties. We show by simulations based on a practical-relevant ITU channel model, that the objective function is well chosen and one can recover the user allocations under the presence of noise. These results enable us to identify non-adjacent spectrum usage belonging to the same transmitter which goes beyond edge-detection strategy usually used in this context.
Low energy electron beam top surface image processing using chemically amplified AXT resist (1997)

High resolution processes are demonstrated with a positive-mode chemically amplified AXT top surface imaging resist system exposed with a low energy electron beam. Top surface imaging is an ideal match to low energy electron beam lithography because it allows thick resist layers to be patterned despite the limited penetration depth of the electron beam. The three key steps of the process are exposure, silylation, and etch development. All three steps influence the final process sensitivity, contrast, and resolution. The AXT has a poly(hydroxy styrene) base resin, and has been formulated both with and without a dye used to enhance optical absorption. We have achieved sub 100 nm resolution both with and without a postexposure bake. Critical area doses below 1 μC/m2 are demonstrated. The edge roughness and density of etch residue from silylation defects have been compared for a variety of oxygen plasma etch systems.
Mechanische Spannung und Valenzabsättigung in Konkurrenz: Nano‐Münzrollen aus Stapeln nanoskaliger Schichten (2010)

Die dritte Dimension kommt bei der Synthese zweidimensionaler Kristalle mit begrenzter Größe zur Hilfe. Entsprechende graphenartige Einzelschichten von Metall(IV)-Chalkogeniden werden durch Stapelung zu Nanoobjekten stabilisiert, die in ihrer Form an Münzrollen erinnern. Die Spannung infolge der Gitterfehlanpassung zwischen NbS2 und WS2 verhindert dabei die Bildung von intrinsisch stabileren aufgerollten Fulleren- oder Nanoröhren-Strukturen. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Phylogeny of Pimeliinae: progress and surprises (2021)

International audience
LE DIAGNOSTIC PRECOCE DES URGENCES NEUROCHIRURGICALES (1979)

SUR LE PLAN NEUROCHIRURGICAL, LES URGENCES SONT DIVISEES EN DEUX SECTEURS. ON LES RETROUVE D'UNE PART DANS LES LESIONS TRAUMATIQUES PRIMAIRES ET D'AUTRE PART DANS DES SITUATIONS AVEC COMPLICATIONS. CERTAINS CAS COMPLEXES DE LESIONS TRAUMATIQUES GRAVES SONT TRAITES PAR L'AUTEUR, AINSI QUE LE TRAITEMENT THERAPEUTIQUE QUI S'Y APPLIQUE (VOIR DIRR 109902).
Table S1 from Impact of the Anticancer Drug NT157 on Tyrosine Kinase Signaling Networks (2023)

&lt;p&gt;Summary of phosphoproteomic profiling data.&lt;/p&gt;
Work hardening response of M50-NiL case hardened bearing steel during shakedown in rolling contact fatigue (2012)

The work hardening response during shakedown phase for case hardened M50-NiL bearing steel in rolling contact fatigue (RCF) is presented. Rolling contact fatigue testing is performed using a ball rod tester for varying cycles and Hertzian stress of 5·5 GPa on multiple test tracks of a case hardened rod. Longitudinal sectioning and polishing of the test rod reveal the RCF affected zones. Micro-Vickers indentation mapping of the subsurface zones is used to measure workhardening. A maximum increase in hardness from 7·4 to 8·3 GPa was observed at ∼13·5×10 6 cycles, after which hardness saturated. A finite element simulation of the RCF test was performed to predict the increase in hardness. It is hypothesised that plastic strain accumulation around the hard carbide inclusions results in increased hardness. Cyclic hardening likely saturates when the ratchet strain per cycle approaches zero. Plastic shakedown follows with no further accumulation of plastic strain and marks the beginning of steady state response.
518: KINDERGARTEN READINESS ASSESSMENT SCORES AFTER MECHANICAL VENTILATION IN THE PICU BEFORE AGE THREE (2021)

Shein, Steven; Sorensen, Alena; Fischer, Meredith; Steh, Stephen; Richter, Francisca Author Information
ChemInform Abstract: Acidity and Alkylation of 4‐Phenyl‐3,5‐dihydroxypyrazole and Its Derivatives. C versus O and N Alkylation. (1989)

Abstract The tautomers of 4‐phenyl‐3,5‐dihydroxypyrazole (I) are alkylated with diazomethane (II) to give the O‐ and N‐methylation products (III) and (IV).
A Cyber Collaborative Protocol for Real-Time Communication and Control in Human-Robot-Sensor Work (2021)

Real-time communication and control are essential parts of the Cyber Physical System (CPS) to optimize effective performance and reliability. To gain a sustainable competitive advantage with Automation 5.0, as needed in Work-of-the-Future, this article addresses the concept of real-time communication and control in the case of an agricultural work setting, along with a newly designed Cyber Collaborative Protocol, called CCP-RTC2. The developed protocol aims to minimize information delay and maximize JIN (Just In Need) information sharing, to enable collaborative decisions among system agents. Two experiments are conducted to compare the designed protocol’s performance in agricultural CPS against the current non-CPS practice. The results demonstrate that the CCP-RTC2 is superior compared with current practice in terms of information sharing in a normal operation scenario. When the system obtains an unplanned request, the CCP-RTC2 can integrate such a request to the original work plan while minimizing the system’s objective function (lower is better). Hence, the system has relatively smaller information delays, as well as better timely information shared with system agents that need it.
Bioactive Microsphere‐Based Scaffolds Containing Decellularized Cartilage (2015)

The aim of this study was to fabricate mechanically functional microsphere-based scaffolds containing decellularized cartilage (DCC), with the hypothesis that this approach would induce chondrogenesis of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro. The DCC was derived from porcine articular cartilage and decellularized using a combination of physical and chemical methods. Four types of scaffolds were fabricated: poly(d,l-lactic-co-glycolic acid) (PLGA) only (negative control), TGF-β-encapsulated (positive control), PLGA surface coated with DCC, and DCC-encapsulated. These scaffolds were seeded with rBMSCs and cultured up to 6 weeks. The compressive modulus of the DCC-coated scaffolds prior to cell seeding was significantly lower than all other scaffold types. Gene expression was comparable between DCC-encapsulated and TGF-β-encapsulated groups. Notably, DCC-encapsulated scaffolds contained 70% higher glycosaminoglyan (GAG) content and 85% more hydroxyproline compared to the TGF-β group at week 3 (with baseline levels subtracted out from acellular DCC scaffolds). Certainly, bioactivity was demonstrated in eliciting a biosynthetic response from the cells with DCC, although true demonstration of chondrogenesis remained elusive under the prescribed conditions. Encapsulation of DCC appeared to lead to improved cell performance relative to coating with DCC, although this finding may be a dose-dependent observation. Overall, DCC introduced via microsphere-based scaffolds appears to be promising as a bioactive approach to cartilage regeneration, although additional studies will be required to conclusively demonstrate chondroinductivity.
Community Extraction in Multilayer Networks with Heterogeneous Community Structure. (2024)

Multilayer networks are a useful way to capture and model multiple, binary or weighted relationships among a fixed group of objects. While community detection has proven to be a useful exploratory technique for the analysis of single-layer networks, the development of community detection methods for multilayer networks is still in its infancy. We propose and investigate a procedure, called Multilayer Extraction, that identifies densely connected vertex-layer sets in multilayer networks. Multilayer Extraction makes use of a significance based score that quantifies the connectivity of an observed vertex-layer set through comparison with a fixed degree random graph model. Multilayer Extraction directly handles networks with heterogeneous layers where community structure may be different from layer to layer. The procedure can capture overlapping communities, as well as background vertex-layer pairs that do not belong to any community. We establish consistency of the vertex-layer set optimizer of our proposed multilayer score under the multilayer stochastic block model. We investigate the performance of Multilayer Extraction on three applications and a test bed of simulations. Our theoretical and numerical evaluations suggest that Multilayer Extraction is an effective exploratory tool for analyzing complex multilayer networks. Publicly available code is available at https://github.com/jdwilson4/MultilayerExtraction.
Assessment of formation damage from drilling fluids dynamic filtration in gas hydrate reservoirs of the North Slope of Alaska (2005)

Thesis (M.S.) University of Alaska Fairbanks, 2005
Molecular dosimetry of N7-(2-hydroxypropyl)guanine in tissues of F344 rats after inhalation exposure to propylene oxide (2003)

Propylene oxide (PO) is a high-volume chemical intermediate that causes a low incidence of nasal tumors in rodents exposed to high concentrations (> or =300 p.p.m.). PO reacts with DNA forming mainly N7-(2-hydroxypropyl)guanine (7-HPG). The exposure-dependent accumulation of 7-HPG in nasal respiratory epithelium (NRE), lung and liver was determined in male F344 rats exposed to PO (0, 5, 25, 50, 300 or 500 p.p.m.) by the inhalation route for 3 or 20 days (6 h/day; 5 days/week). These exposures ranged from low concentrations, such as those potentially occurring in the workplace, to high concentrations that proved to be carcinogenic in rodents. Analysis of 7-HPG in DNA by gas chromatography-high-resolution mass spectrometry (GC-HRMS) showed a linear response in 7-HPG for all three tissues after 3 days of exposure, and for NRE and lung after 20 days of exposure. A slightly sublinear response in 7-HPG was observed in liver after 20 days of exposure. For both exposure periods, the NRE had the highest concentration of 7-HPG, followed by lung and liver. The amount of 7-HPG in NRE was seven and 17 times higher than in lung and liver, respectively, for the 3 day exposures. For the 20 day exposures, the concentration of 7-HPG in NRE was six and 13 times higher than that in lung and liver, respectively, over the concentration range studied. These results demonstrate a much higher extent of DNA alkylation in the target tissue for carcinogenesis, than in non-target tissues. As PO-induced tumor formation was highly sublinear, occurring only at high vapor concentrations, whereas 7-HPG adducts were shown to be linearly dependent on airborne concentration, these results suggest that 7-HPG is not sufficient for PO nasal carcinogenesis and that other factors such as increased cell proliferation may be important in determining the tumor exposure response.
Paraganglioma with High Levels of Dopamine, Dopa Decarboxylase Suppression, Dopamine β-hydroxylase Upregulation and Intra-tumoral Melanin Accumulation: A Case Report with a Literature Review (2022)

Object Exclusively dopamine-producing pheochromocytoma/paraganglioma (PPGL) is an extremely rare subtype. In this condition, intratumoral dopamine β-hydroxylase (DBH), which controls the conversion of norepinephrine from dopamine, is impaired, resulting in suppressed norepinephrine and epinephrine production. However, the rarity of this type of PPGL hampers the understanding of its pathophysiology. We therefore conducted genetic and immunohistological analyses of a patient with an exclusively dopamine-producing paraganglioma. Methods Paraganglioma samples from a 52-year-old woman who presented with a 29.6- and 41.5-fold increase in plasma and 24-h urinary dopamine, respectively, but only a minor elevation in the plasma norepinephrine level was subjected to immunohistological and gene expression analyses of catecholamine synthases. Three tumors carrying known somatic PPGL-related gene variants (HRAS, EPAS1) were used as controls. Whole-exome sequencing (WES) was also performed using the patient's blood and tumor tissue. Results Surprisingly, the protein expression of DBH was not suppressed, and its mRNA expression was clearly higher in the patient than in the controls. Furthermore, dopa decarboxylase (DDC), which governs the conversion of 3,4-dihydroxyphenyl-<sub>L</sub>-alanine (<sub>L</sub>-DOPA) to dopamine, was downregulated at the protein and gene levels. In addition, melanin, which is synthesized by <sub>L</sub>-DOPA, accumulated in the tumor. WES revealed no PPGL-associated pathogenic germline variants, but a missense somatic variant (c.1798G>T) in CSDE1 was identified. Conclusion Although pre-operative plasma <sub>L</sub>-DOPA was not measured, our histological and gene expression analyses suggest that <sub>L</sub>-DOPA, rather than dopamine, might have been overproduced in the tumor. This raises the possibility of pathophysiological heterogeneity in exclusively dopamine-producing PPGL.
Diphenylcarbinyl derivatives. II. Solvolysis of some disubstituted benzhydryl chlorides. Examination of the additivity relationship (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDiphenylcarbinyl derivatives. II. Solvolysis of some disubstituted benzhydryl chlorides. Examination of the additivity relationshipShinya NishidaCite this: J. Org. Chem. 1967, 32, 9, 2695–2697Publication Date (Print):September 1, 1967Publication History Published online1 May 2002Published inissue 1 September 1967https://pubs.acs.org/doi/10.1021/jo01284a010https://doi.org/10.1021/jo01284a010research-articleACS PublicationsRequest reuse permissionsArticle Views85Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
EPR/ENDOR, Mössbauer, and Quantum-Chemical Investigations of Diiron Complexes Mimicking the Active Oxidized State of [FeFe]Hydrogenase (2012)

Understanding the catalytic process of the heterolytic splitting and formation of molecular hydrogen is one of the key topics for the development of a future hydrogen economy. With an interest in elucidating the enzymatic mechanism of the [Fe(2)(S(2)C(2)H(4)NH)(CN)(2)(CO)(2)(μ-CO)] active center uniquely found in [FeFe]hydrogenases, we present a detailed spectroscopic and theoretical analysis of its inorganic model [Fe(2)(S(2)X)(CO)(3)(dppv)(PMe(3))](+) [dppv = cis-1,2-bis(diphenylphosphino)ethylene] in two forms with S(2)X = ethanedithiolate (1edt) and azadithiolate (1adt). These complexes represent models for the oxidized mixed-valent Fe(I)Fe(II) state analogous to the active oxidized "H(ox)" state of the native H-cluster. For both complexes, the (31)P hyperfine interactions were determined by pulse electron paramagnetic resonance and electron nuclear double resonance (ENDOR) methods. For 1edt, the (57)Fe parameters were measured by electron spin-echo envelope modulation and Mössbauer spectroscopy, while for 1adt, (14)N and selected (1)H couplings could be obtained by ENDOR and hyperfine sublevel correlation spectroscopy. The spin density was found to be predominantly localized on the Fe(dppv) site. This spin distribution is different from that of the H-cluster, where both the spin and charge densities are delocalized over the two Fe centers. This difference is attributed to the influence of the "native" cubane subcluster that is lacking in the inorganic models. The degree and character of the unpaired spin delocalization was found to vary from 1edt, with an abiological dithiolate, to 1adt, which features the authentic cofactor. For 1adt, we find two (14)N signals, which are indicative for two possible isomers of the azadithiolate, demonstrating its high flexibility. All interaction parameters were also evaluated through density functional theory calculations at various levels.
Near-Hover Flapping Wing MAV Aerodynamic Modelling: A linear model approach (2013)

Flapping Wing Micro Air Vehicles typically have to be controlled actively in order to fly autonomously. Such active control benefits significantly from a model relating the flapper’s state and control actions to the resulting forces and moments. This article presents the first results on system identification of a 17g ornithopter, the Delfly II. In particular, the Delfly II was programmed to perform specific flight manoeuvres in a high-fidelity sub-millimetre resolution external tracking system flight arena. The states were reconstructed and used to calculate the aerodynamic forces and moments that acted on the Delfly, under the assumptions of a rigid body and constant inertia properties, using general aircraft equations of motion. These forces and moments were used to identify parameters of a linear model around a trimmed hover condition. Two linear models are devised and compared: (1) a full model that incorporated state variables that were reconstructed from the tracking system, and (2) a reduced model that only included state variables that can be determined from onboard sensors. Using the linear time-invariant models it has been possible to estimate the aerodynamic forces with a very good approximation. However, neither the full nor the reduced models reveal to be able to estimate the aerodynamic moments as well as they do for the aerodynamic forces. The results point to the possibility of linear model use to control the Delfly in close-to-hover regime in closed loop.
Autoregressive parameters for a suite of strong-motion accelerograms (1979)

abstract The time-adaptive technique of Jurkevics and Ulrych (1978) has been used to analyze a suite of rock-site accelerograms recorded during two ML = 6.4 earthquakes in California. One of the parameters which models the earthquake, the innovation variance σe2, has been found to vary with epicentral distance according to (σe2)max ∝ D-3.23. The shaking duration exhibits a linear variation with log(D) after a finite distance away from the fault. The above results allow the construction of artificial accelerograms for arbitrary epicentral distances ML = 6.4 earthquakes in California.
Imparting Antimicrobial and Antifouling Properties to Anion Exchange Membrane through the Modification with Gentamicin‐Based Polymer (2021)

Abstract A series of gentamicin‐modified anion exchange membranes (AEMs) immobilized by mussel‐inspired dopamine coating is prepared in this work. The purpose is to improve antibacterial activity and fouling resistance of the commercial AEMs (AEM Type I, Fujifilm Co.). As a result of the applied modification, the hydrophilicity of membrane surface improved significantly. Thus, the as‐prepared modified AEMs exhibit the good antifouling property, in electrodialysis (ED) experiments by using the sodium dodecylbenzenesulfonate and albumin from bovine serum as model foulants. Furthermore, taking into account the results of the antibacterial test, the resulting modified AEMs exhibit improved antibacterial property for Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). After a brief contact with bacteria, the antibacterial rates of modified AEMs coating with gentamicin‐based polymer are 80.11% for E. coli and 90.66% for S. aureus , which are much higher than pristine AEM (6.28% and 9.07%), respectively. In addition, the modified membrane has good operational stability during ED process and recovery ability after contamination, and also maintains satisfactory electrochemical properties. This investigation highlights a design of simple strategy for the preparation of antimicrobial and antifouling AEM, which can be used for ED.