gebhart/patrepeval huggingface dataset:
Latent human cytomegalovirus enhances HIV-1 infection in CD34+ progenitor cells (2017)

Individuals who have been preinfected by human cytomegalovirus (HCMV) are more prone to AIDS disease progression after subsequent HIV-1 infection but the underlying mechanism remains elusive. HCMV is a ubiquitous DNA virus that commonly establishes lifelong latent infection in CD34<sup>+</sup> progenitor cells, where latency-specific HCMV genes may modulate host restriction to HIV-1 infection. To test this hypothesis, we studied progenitor cells that are known to resist replicative HIV-1 infection because of the intrinsic expression of host restriction factors. Interestingly, in primary CD34<sup>+</sup> cells undergoing latent HCMV infection, an enhanced level of HIV-1 proviral DNA and replication was observed as measured by digital polymerase chain reaction, quantitative polymerase chain reaction, and Gag expression, and confirmed using dual-reporter pseudovirus encoding X4- or R5-tropic envelope and T-cell transfer. This phenomenon may be partially explained by the upregulation of HIV-1 entry coreceptors, including chemokine receptors CXCR4 and CCR5, but not of the primary receptor CD4. Furthermore, latent HCMV infection downregulated the expression of HIV-1 restriction factors SAMHD1, APOBEC3G, tetherin, and Mx2 in CD34<sup>+</sup> progenitor cells, which may confer to enhanced HIV-1 infection. However, this enhancement was abrogated when ultraviolet-inactivated HCMV was used for comparison, suggesting that expression of latent HCMV genes is essential for this effect. Importantly, HCMV gB and HIV-1 p24 can be detected in the same cell by immunofluorescence and flow cytometry; therefore, the establishment of HCMV latency in CD34<sup>+</sup> cells likely leads to host cell gene modulation that favors HIV-1 infection.
Visual and Instrumental Colorimetric Assessments of Small Color Differences on Translucent Dental Porcelain (1989)

The CIELAB colorimetric system was used for the study of the relationship between measured color difference values and human observer responses. This study verified that a specific, visually meaningful and precise relationship exists between the magnitude and direction of the measurements and the average dental-observer responses. The results support the use of this system in dentistry as a means of evaluating color by differences. The further development of such systems for clinical use would be warranted and could serve as a valuable tool for material selection and restoration design, particularly in the area of aesthetic restorative dentistry.
1088: Novel Bispecific Antisense Oligonucleotides Inhibiting both BCL-2 and BCL-XL Expression Induce Apoptosis and Enhance Chemosensitivity in Human Androgen-Independent Prostate Cancer Cells (2004)

You have accessJournal of UrologyDiscussed Poster, Monday, May 10, 2004, 1:00 - 5:00 pm1 Apr 20041088: Novel Bispecific Antisense Oligonucleotides Inhibiting both BCL-2 and BCL-XL Expression Induce Apoptosis and Enhance Chemosensitivity in Human Androgen-Independent Prostate Cancer Cells Kazuki Yamanaka, Hideaki Miyake, Uwe Zangemeister-Wittke, Burkhard Jansen, and Martin E. Gleave Kazuki YamanakaKazuki Yamanaka More articles by this author , Hideaki MiyakeHideaki Miyake More articles by this author , Uwe Zangemeister-WittkeUwe Zangemeister-Wittke More articles by this author , Burkhard JansenBurkhard Jansen More articles by this author , and Martin E. GleaveMartin E. Gleave More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)38325-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "1088: Novel Bispecific Antisense Oligonucleotides Inhibiting both BCL-2 and BCL-XL Expression Induce Apoptosis and Enhance Chemosensitivity in Human Androgen-Independent Prostate Cancer Cells." The Journal of Urology, 171(4S), p. 287 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 171Issue 4SApril 2004Page: 287 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Kazuki Yamanaka More articles by this author Hideaki Miyake More articles by this author Uwe Zangemeister-Wittke More articles by this author Burkhard Jansen More articles by this author Martin E. Gleave More articles by this author Expand All Advertisement PDF downloadLoading ...
The short-term impact of probiotic consumption on the oral cavity microbiome (2018)

The dysbiosis of the oral microbiome is associated with both localized and systemic diseases. Modulating the resident microbial communities by the dietary consumption of probiotics has become an appealing means to promote host health by either restoring host-microbe balance or preventing dysbiosis. Most probiotics strategies target the intestinal microbiome, but little is known about their impact on the oral microbiome. We analyzed here the saliva microbiome from 21 volunteers, longitudinally collected before, during, and after consumption of a commercial probiotic and a standard yoghurt using 16S amplicon sequencing. The alpha diversity of the saliva microbiome had a statistically significant increase (P-value = 0.0011) in one of the groups that consumed the probiotic. The overall structure of the microbiome was however not significantly impacted by the probiotic, although oligotyping analysis revealed that both Streptococci and Lactobacilli present in the probiotic product persisted in the saliva microbiome. In contrast, non-probiotic yoghurt consumption had a lesser impact on the overall diversity and Lactobacillus and Streptococcus persistence. Our results suggest that consumption of commercial probiotics in healthy subjects increase the overall diversity of the oral cavity microbiome in the short term, but such dietary interventions are not able to substantially modify the structure of the microbiome.
Capturing of intracranial pressure treatment during neurointensive care in patients with acute brain injury using a novel tablet-based method (2022)

Critical care is complex and stressful. It is difficult to register in real time data not recorded by automatic systems. Time-specific knowledge of manual measures is important for understanding pathophysiology and for analyzing treatment and quality of care. Therefore, a novel iPad-based method for registration of manual measures was developed, which many can build themselves. Using a configuration for intracranial pressure (ICP) management, the methodology was validated, ICP treatment captured, and the quality of ICP management evaluated. Twenty-two patients with acute brain injuries were studied. The iPad-system was totally used for 2538 h. Thirteen-hundred-five manual measures were entered. Thirty-nine episodes of predefined ICP insults were identified. During 16/39 episodes, ICP treatments were registered. For 4/39 episodes treatments were registered within 90 s before or after the episode. For 3/39 episodes it was registered that treatment was intentionally refrained. In 15/16 episodes without registered treatment, the insult was mild or reasonable explanations were found when medical records and the Patient data management system were reviewed. In one situation without particular circumstances, morphine and clonidine were given to decrease ICP but not registered. No episodes of downtime or loss of data occurred. The developed methodology appears to be stable and robust as well as feasible and user-friendly. It was possible to capture the treatment of ICP insults with high temporal resolution, and to evaluate the quality of ICP management. An own developed novel tablet-based system like our system may be a promising potential tool useful in various future intensive care applications.
Ultimate Turbulent Taylor-Couette Flow (2012)

The flow structure of strongly turbulent Taylor-Couette flow with Reynolds numbers up to Re(i)=2×10(6) of the inner cylinder is experimentally examined with high-speed particle image velocimetry (PIV). The wind Reynolds numbers Re(w) of the turbulent Taylor-vortex flow is found to scale as Re(w)∝Ta(1/2), exactly as predicted by Grossmann and Lohse [Phys. Fluids 23, 045108 (2011).] for the ultimate turbulence regime, in which the boundary layers are turbulent. The dimensionless angular velocity flux has an effective scaling of Nu(ω)∝Ta(0.38), also in correspondence with turbulence in the ultimate regime. The scaling of Nu(ω) is confirmed by local angular velocity flux measurements extracted from high-speed PIV measurements: though the flux shows huge fluctuations, its spatial and temporal average nicely agrees with the result from the global torque measurements.
A peptide composed of tandem analogs of two myasthenogenic T cell epitopes interferes with specific autoimmune responses (1997)

Myasthenia gravis (MG) is a T cell-regulated, antibody-mediated autoimmune disease. Two peptides representing sequences of the human acetylcholine receptor alpha-subunit, p195-212 and p259-271, were previously shown to stimulate peripheral blood lymphocytes of patients with MG and were found to be immunodominant T cell epitopes in SJL and BALB/c mice, respectively. Single amino acid substituted analogs of p195-212 (analog Ala-207) and p259-271 (analog Lys-262) were synthesized. We showed that analogs Ala-207 and Lys-262 inhibited, in vitro and in vivo, the proliferative responses of T cell lines specific to the relevant peptide and lymph node cells of mice immunized to p195-212 and p259-271, respectively. To inhibit T cell responses to both peptides (p195-212 and p259-271), we synthesized dual analogs composed of the tandemly arranged two single (Ala-207 and Lys-262) analogs (dual analog) either sequentially (Ala-207-Lys-262) or reciprocally (Lys-262-Ala-207). In the present study, we report that both dual analogs could bind to major histocompatibility complex class II molecules on antigen-presenting cells of SJL and BALB/c mice. Analog Lys-262-Ala-207, which bound more efficiently to major histocompatibility complex class II molecules, was found to inhibit the proliferative responses of both p195-212- and p259-271-specific T cell lines. Furthermore, the analog inhibited the in vivo priming of lymph node cells of both SJL and BALB/c mice when administered i.v., i.p., or per os. The dual analog Lys-262-Ala-207 could also immunomodulate myasthenogenic manifestations in mice with experimental autoimmune MG induced by inoculation of a pathogenic T cell line. Thus, a single peptide that is composed of analogs to two epitope specificities can be used to regulate T cell responses and disease associated with each epitope.
The Lek-like Mating System of Halichoeres melanochir (Pisces: Labridae) at Miyake-jima, Japan1 (2010)

Reproductive behavior and social organization of the labrid fish Halichoeres melanochir were studied at Miyake-jima, Izu Islands, Japan. ♂♂ set up contiguous territories from early to mid-afternoon daily during the breeding season, with ♂♂ at the center of the spawning site experiencing significantly higher spawning success than those at the periphery. Unlike other labrids with lek-like mating systems previously reported, H. melanochir is sexually monochromatic and shows only minimal sexual size-dimorphism. The spawning behavior and mating system are described and compared with current theories on labrid reproductive strategies.
Nitric oxide reacts with the ferryl‐oxo catalytic intermediate of the CuB‐lacking cytochrome bd terminal oxidase (2006)

Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized enzyme with nitrite bound to heme d at the active site. Unexpectedly, the NO reaction mechanism of this catalytic intermediate in the Cu(B)-lacking cytochrome bd appears similar to that of beef heart cytochrome c oxidase, where Cu(B) was proposed to play a key role.
Parallel Algorithms for Refutation Tree Problem on Formal Graph Systems (1995)

We define a new framework for rewriting graphs, called a formal graph system (FGS), which is a logic program having hypergraphs instead of terms in first-order logic. We first prove that a class of graphs is generated by a hyperedge replacement grammar if and only if it is defined by an FGS of a special form called a regular FGS. In the same way as logic programs, we can define a refutation tree for an FGS. The classes of TTSP graphs and outerplanar graphs are definable by regular FGSs. Then, we consider the problem of constructing a refutation tree of a graph for these FGSs. For the FGS defining TTSP graphs, we present a refutation tree algorithm of $O left(log^2 n+log m \\right)$ time with $O left(n+m \\right)$ processors on an EREW PRAM. For the FGS defining outerplanar graphs, we show that the refutation tree problem can be solved in $O left(log^2 n \\right)$ time with $Oleft(n+rn)$ processors on an EREW PRAM. Here, n and m are the numbers of vertices and edges of an input graph, respectively.
Influence of respiration on heart rate and blood pressure fluctuations (1993)

The dynamics of the respiratory and cardiovascular systems were studied by continuously slowing respiration from 0.46 to 0.05 Hz. The time-frequency distribution and global spectral analysis were used to assess the R-R interval (R-R) and the systolic and diastolic blood pressure fluctuations in 16 healthy subjects. During rest, the nonrespiratory-to-respiratory frequency ratios were not affected by occasional slow breathing, whereas the low- (0.01-0.15 Hz) to high- (0.15-0.3 Hz) frequency indexes for blood pressure were increased (P < 0.05). The respiratory fluctuations in R-R and the systolic and diastolic pressures were paced over the 0.46- to 0.05-Hz range. As respiration slowed to 0.07-0.09 Hz, the frequency content of the respiration and cardiovascular variables increased sharply and nonlinearly to a maximum that exceeded values at higher frequencies (P < 0.001). The nonrespiratory frequency content remained stable in the 0.01- to 0.05-Hz range and did not significantly differ from that at rest. In contrast, the nonstable 0.05- to 0.1-Hz component was suppressed. A slow 0.012- to 0.017-Hz rhythm modulated respiration and hemodynamic fluctuations at both respiratory and nonrespiratory frequencies. The study indicated that respiration input should be considered in the interpretation of global spectra. Furthermore the time-frequency distributions demonstrated that a close nonlinear coupling exists between the respiratory and cardiovascular systems.
Structure-Based Survey of the Human Proteome for Opportunities in Proximity Pharmacology (2022)

ABSTRACT Proximity pharmacology (ProxPharm) is a novel paradigm in drug discovery where a small molecule brings two proteins in close proximity to elicit a signal, generally from one protein onto another. The potential of ProxPharm compounds as a new therapeutic modality is firmly established by proteolysis targeting chimeras (PROTACs) that bring an E3 ubiquitin ligase in proximity to a target protein to induce ubiquitination and subsequent degradation of the target protein. The concept can be expanded to induce other post-translational modifications via the recruitment of different types of protein-modifying enzymes. To survey the human proteome for opportunities in proximity pharmacology, we systematically mapped non-catalytic drug binding pockets on the structure of protein-modifying enzymes available from the Protein Databank. In addition to binding sites exploited by previously reported ProxPharm compounds, we identified putative ligandable non-catalytic pockets in 188 kinases, 42 phosphatases, 26 deubiquitinases, 9 methyltransferases, 7 acetyltransferases, 7 glycosyltransferases, 4 deacetylases, 3 demethylases and 2 glycosidases, including cavities occupied by chemical matter that may serve as starting points for future ProxPharm compounds. This systematic survey confirms that proximity pharmacology is a versatile modality with largely unexplored and promising potential, and reveals novel opportunities to pharmacologically rewire molecular circuitries.
Synchronized Operation of DSP-Based Generalized Impedance Controller With Variable-Speed Isolated SEIG for Novel Voltage and Frequency Control (2014)

In this paper, the performance of a novel two-loop control strategy for a three-phase self-excited induction generator (SEIG), used in wind/microhydro/biogas-based isolated generation schemes, is presented. The implementation of the grid-interactive synchronization of the generalized impedance controller (GIC), an impedance-controlled operation of pulse width modulation (PWM) voltage-source converter, with SEIG is described. The SEIG frequency is tracked using digital phase-locked loop implemented on a dSpace DSP platform. The open-loop SEIG-GIC system is experimentally analyzed for the sensitivity of the SEIG voltage and frequency to GIC modulation index m and phase angle δ. Based on this, a simple and practical control strategy with proportional-integral (PI)-based voltage and frequency feedback loops is evolved for the regulation of the SEIG voltage and frequency. A novel design procedure for both the PI controllers for stable closed-loop operation is presented. The performance of the proposed closed-loop system is experimentally tested under speed and load perturbations.
Orthotopic Neobladder Following Cystectomy (2001)

In Brief Gross painless hematuria often prompts patients to seek medical attention. For some persons, especially those aged 55 to 80 years, this hematuria will prove to be the result of bladder cancer. Patients found to have muscle-invasive disease commonly undergo radical cystectomy and urinary diversion. The decision about which urinary diversion will be best suited to the individual patient is complex and depends on multiple factors relating to the patient and the tumor as well as a clear understanding about the risks and benefits of each diversion. This article will discuss issues such as cancer control, preoperative and postoperative care, management of the condition at home, and quality of life associated with the various urinary diversion options, with a special emphasis on orthotopic neobladder reconstruction. Awareness of the special issues associated with this procedure is needed to safely counsel and care for patients with these forms of bladder substitution. The preoperative and postoperative client teaching and the surgical procedure for an orthotopic neobladder reconstruction.
Intramolecular Hydrogen Bonding and Molecular Structure of 2,5-Dihydroxyterephthalaldehyde and 4,6-Dihydroxyisophthalaldehyde:  A Gas-Phase Electron Diffraction and ab Initio Molecular Orbital Study (1996)

The molecular structure of 2,5-dihydroxyterephthalaldehyde has been determined from a joint electron diffraction/ab initio investigation, and the molecular structure of 4,6-dihydroxyisophthalaldehyde has been obtained from ab initio calculations at the MP2/6-31G* level. There is considerable intramolecular hydrogen bonding in these structures manifested by the O···H and O···O distances as well as by the structural changes in the rest of the molecule. These changes are consistent with the notion of resonance-assisted hydrogen bonding. The hydrogen bonding is somewhat stronger in 4,6-dihydroxyisophthalaldehyde than in 2,5-dihydroxyterephthalaldehyde, and this difference may be linked to the difference in the mutual positioning of the interacting formyl and hydroxy groups in these molecules.
Food waste treatment using Bacillus species isolated from food wastes and production of air-dried Bacillus cell starters (2018)

The objectives of this research were to 1) isolate and identify thermophilic bacteria for food waste treatment; 2) investigate the capability of food waste treatment using Bacillus species; and 3) develop air-dried Bacillus starters for food waste treatment. Five Bacillus species were isolated from food wastes and identified as Bacillus licheniformis (B. licheniformis) G1, Bacillus circulans C2, Bacillus subtilis (B. subtilis) E1, Bacillus vanillea F1, and Bacillus atrophaeus G2 based on 16S rDNA sequencing. Each identified Bacillus and the mixture of Bacillus species were cultivated in the standard food waste at 45C for 8 d. Changes in cell count, solid contents, and pH of the food waste were monitored during cultivation. Air-dried Bacillus cell powders were prepared using wheat flour and lactomil as excipients, and the cell count and survival rate were determined. The cell count of B. licheniformis G1 exhibited the highest number among the tested Bacillus (~10 8 CFU/mL). The greatest reduction in solid contents of food waste was achieved by B. subtilis E1 (22.6%). The mixture of B. licheniformis G1 and B. subtilis E1 exhibited a synergistic effect on the reduction of solid contents. Lactomil was determined as better excipient than wheat flour based on the greatest survival rate of 95%.
Nb ‐Modified 0.9 BaTiO 3 –0.1( Bi 0.5 Na 0.5 ) TiO 3 Ceramics for X 9 R High‐Temperature Dielectrics Application Prepared by Coating Method (2012)

0.9 BaTiO 3 –0.1 Bi 0.5 Na 0.5 TiO 3 ‐based (0.9 BT –0.1 BNT ) ceramics with T c = 180°C were prepared using a novel chemical coating method. The relationships among compositions, microstructures, and properties were investigated. TEM morphologies displayed the Nb ‐coated powders, which were confirmed by energy dispersive spectroscopy ( EDS ). Temperature‐dependence of permittivity for Nb ‐modified 0.9 BT –0.1 BNT ceramics measured at different frequencies showed no relaxor characteristic at high temperature, but frequency dispersion at low temperature. The microstructures were characterized by two types of grains: Nb ‐rich grains and Nb ‐free grains, which were responsible for temperature‐stable dielectric properties. The sample with 5 mol% Nb addition satisfied the X 9 R specification. The permittivity was 1934 and the dissipation factor was 1.53% at room temperature.
Slx1—Slx4 is a second structure-specific endonuclease functionally redundant with Sgs1—Top3 (2003)

The RecQ DNA helicases human BLM and yeast Sgs1 interact with DNA topoisomerase III and are thought to act on stalled replication forks to maintain genome stability. To gain insight into this mechanism, we previously identified SLX1 and SLX4 as genes that are required for viability and for completion of rDNA replication in the absence of SGS1-TOP3. Here we show that SLX1 and SLX4 encode a heteromeric structure-specific endonuclease. The Slx1-Slx4 nuclease is active on branched DNA substrates, particularly simple-Y, 5'-flap, or replication fork structures. It cleaves the strand bearing the 5' nonhomologous arm at the branch junction and generates ligatable nicked products from 5'-flap or replication fork substrates. Slx1 is the founding member of a family of proteins with a predicted URI nuclease domain and PHD-type zinc finger. This subunit displays weak structure-specific endonuclease activity on its own, is stimulated 500-fold by Slx4, and requires the PHD finger for activity in vitro and in vivo. Both subunits are required in vivo for resistance to DNA damage by methylmethane sulfonate (MMS). We propose that Sgs1-Top3 acts at the termination of rDNA replication to decatenate stalled forks, and, in its absence, Slx1-Slx4 cleaves these stalled forks.
Ethno-marketing of pottery : Fieldwork in the Faro department, Northern Cameroon (1996)

L'etude des systemes d'ethno-marketing de la production ceramique prend place dans le Ceramic and Society Project initie en 1994 par l'Universite de Bruxelles. Cette etude consiste a analyser, d'un point de vue ethno-archeologique, la vie d'un objet, en l'occurrence de la ceramique, de sa production a son abandon, en evaluant l'impact de facteurs tels que la production, la vente, l'echange et les modalites de repartition sur l'ensemble des modeles de consommation. L'article presente les resultats d'une telle etude appliquee au materiel ceramique collecte dans le departemet de Faro au Cameroun en 1996