gebhart/patrepeval huggingface dataset:
Detection of four different 11q23 chromosomal abnormalities by multiplex-PCR and fluorescence-based automatic DNA-fragment analysis. (1995)

A nested polymerase chain reaction (PCR) protocol was developed for rapid detection of four different 11q23 abnormalities by a single PCR assay. During each of the two PCR rounds a sense primer located within exon 5 of the MLL gene at 11q23 was combined with four different antisense primers, each located within possible translocation partner genes at chromosomes 4, 6, 9, and 19, respectively. Except for the MLL primer all primers used during the second round of nested-PCR carried a characteristic fluorescence label at their 5'-end. Agarose gel analysis of the PCR products was sufficient to discriminate between the absence of any of the four MLL rearrangements and the presence of at least one of them. Discrimination of the four different MLL translocation partner genes was not possible by agarose gel analysis due to a molecular heterogeneity of the 11q23 breakpoints resulting in PCR products of variable size. For this reason, automatic fluorescence-based DNA-fragment analysis was used to exactly define the MLL translocation partner genes if a positive result had been obtained by agarose gel analysis. In patients with leukemia, this assay may enable a fast and highly sensitive detection of different 11q23 abnormalities, which usually correlate with poor clinical prognosis.
QUARTERLY BOOK - LIST (1943)

Journal Article QUARTERLY BOOK - LIST Get access EDWARD N. WATERS EDWARD N. WATERS Search for other works by this author on: Oxford Academic Google Scholar The Musical Quarterly, Volume XXIX, Issue 2, April 1943, Pages 269–272, https://doi.org/10.1093/mq/XXIX.2.269 Published: 01 April 1943
In Search of Small Molecules That Selectively Inhibit MBOAT4 (2021)

Ghrelin is a 28-residue peptide hormone produced by stomach P/D1 cells located in oxyntic glands of the fundus mucosa. Post-translational octanoylation of its Ser-3 residue, catalyzed by MBOAT4 (aka ghrelin <i>O</i>-acyl transferase (GOAT)), is essential for the binding of the hormone to its receptor in target tissues. Physiological roles of acyl ghrelin include the regulation of food intake, growth hormone secretion from the pituitary, and inhibition of insulin secretion from the pancreas. Here, we describe a medicinal chemistry campaign that led to the identification of small lipopeptidomimetics that inhibit GOAT in vitro. These molecules compete directly for substrate binding. We further describe the synthesis of heterocyclic inhibitors that compete at the acyl coenzyme A binding site.
Molecular characterization of field infectious bursal disease virus isolates from Nigeria (2016)

Phylogenetic analysis of partial sequences of genome segment A and B of IBDV in Nigeria confirmed the existence of vvIBDV in Nigeria. In addition, we noted the existence of reassortant IBDV strains with novel triplet amino acid motifs at positions 145, 146 and 147 in the reassorted Nigerian IBDV.
Chemical Speciation and Dynamics in the Surface Combustion Zone of Energetic Materials (2002)

An overview is presented of the status of chemical measurements of the condensed phase and the near-field gaseous phase of decomposing energetic materials. Various categories of energetic materials are surveyed first. Theexperimental and computational approaches for specifying the most important chemical reactions and rates in the condensed and near-surface gaseous phase are discussed along with the use of these chemical data in qualitative combustion models. Emphasis in the presentation is placed on the nitramines HMX and RDX.
Optimization of Oncolytic Viral Therapy for Hepatocellular Carcinoma (2011)

Oncolytic virotherapy, which involves the use of replication-competent viruses that selectively replicate in tumor cells, represents a novel alternative approach for cancer treatment and has been under intense development over the last two decades. This work focuses on three different strategies for optimizing oncolytic viral therapy for hepatocellular carcinoma, namely combination therapy, viral engineering, and mechanistic investigations to better understand the basis for tumor-specific virus replication. These three approaches resulted in an enhancement of oncolytic viral therapy and provided a potential molecular marker for characterizing a tumor with regard to its amenability to viral therapy.
Paradoxical Effect of Nonphysiological Shear Stress on Platelets and von Willebrand Factor (2015)

Blood can become hypercoagulable by shear-induced platelet activation and generation of microparticles. It has been reported that nonphysiological shear stress (NPSS) could induce shedding of platelet receptor glycoprotein (GP) Ibα, which may result in an opposite effect to hemostasis. The aim of this study was to investigate the influence of the NPSS on platelets and von Willebrand factor (vWF). Human blood was exposed to two levels of NPSS (25 Pa, 125 Pa) with an exposure time of 0.5 s, generated by using a novel blood-shearing device. Platelet activation (P-selectin expression, GPIIb/IIIa activation and generation of microparticles) and shedding of three platelet receptors (GPIbα, GPVI, GPIIb/IIIa) in sheared blood were quantified using flow cytometry. Aggregation capacity of sheared blood induced by ristocetin and collagen was evaluated using an aggregometer. Shear-induced vWF damage was characterized with Western blotting. Consistent with the published data, the NPSS caused significantly more platelets to become activated with increasing NPSS level. Meanwhile, the NPSS induced the shedding of platelet receptors. The loss of the platelet receptors increased with increasing NPSS level. The aggregation capacity of sheared blood induced by ristocetin and collagen decreased. There was a loss of high molecular weight multimers (HMWMs) of vWF in sheared blood. These results suggest that the NPSS induced a paradoxical effect. More activated platelets increase the risk of thrombosis, while the reduction in platelet receptors and the loss of HMWM-vWF increased the propensity of bleeding. The finding might provide a new perspective to understand thrombosis and acquired bleeding disorder in patients supported with blood contacting medical devices.
Origin of Critical Substrate Bias in Variable Threshold Voltage Complementary MOS (VTCMOS) (2002)

The origin of the critical substrate bias (Vo), which is one of the most important parameters for optimum device design in the variable threshold voltage complementary metal oxide semiconductor (VTCMOS), has been systematically investigated and clarified by means of device simulation. Vo is regarded as the substrate bias required to compensate for the degraded drivability in devices with a large body effect factor (γ), by a larger threshold voltage shift (ΔVth). It is found that Vo has two components, which compensate for a large S factor and small transconductance, respectively, and depends on the stand-by off-current (Ioff,standby) and the supply voltage (Vdd). It is also found that as the technology advances, Vo will decrease due to the increase of Ioff,standby and the reduction of Vdd, indicating that VTCMOS with large γ devices will maintain its advantage in the future.
Magnetic properties of quasi one-dimensional vanadium-benzene nanowire affected by gas molecules: a first-principle study (2017)

Magnetic properties of quasi one-dimensional vanadium-benzene nanowires (VBNW) are investigated theoretically with the absorption of gas molecules-NO and NO2. With the increase adsorption of NO on VBNW, the phase transition from half metal to ferromagnetic metal and last to paramagnetic semiconductor can be observed. With the increase of NO2 on VBNW, half metallic property can be enhanced at first and decreased later. Thus, the electronic and magnetic properties of VBNW can be sensitive and selective to NO and NO2, revealing the potential applications in spintronic sensors of these two kinds of molecules.
Fast track endoscopy: Risikofaktoren der ambulanten colorektalen Polypektomie (2009)

Einleitung: Die colorektale Polypektomie hat sich im Zeitalter des ambulanten Operierens zu einer Routinemaßnahme im ambulanten Kliniksektor sowie mehr noch im niedergelassenen Bereich im Sinne einer „fast track endoscopy“ (in Anlehnung an die minimal-invasive Chirurgie) entwickelt. Daher müssen Möglichkeiten und Grenzen der ambulanten endoskopischen Polypektomie abschätzbar sein, um das Risiko der Patientengefährdung zu minimieren.
K. D. Reynolds, Aristocratic women and political society in Victorian Britain . (Oxford: Clarendon Press, 1998.) Pages viii+260. £35.00. (2000)

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Clearance of Heparan Sulfate and Attenuation of CNS Pathology by Intracerebroventricular BMN 250 in Sanfilippo Type B Mice (2017)

Sanfilippo syndrome type B (mucopolysaccharidosis IIIB), caused by inherited deficiency of α-<i>N</i>-acetylglucosaminidase (NAGLU), required for lysosomal degradation of heparan sulfate (HS), is a pediatric neurodegenerative disorder with no approved treatment. Intracerebroventricular (ICV) delivery of a modified recombinant NAGLU, consisting of human NAGLU fused with insulin-like growth factor 2 (IGF2) for enhanced lysosomal targeting, was previously shown to result in marked enzyme uptake and clearance of HS storage in the <i>Naglu</i><sup><i>-/-</i></sup> mouse brain. To further evaluate regional, cell type-specific, and dose-dependent biodistribution of NAGLU-IGF2 (BMN 250) and its effects on biochemical and histological pathology, <i>Naglu</i><sup><i>-/-</i></sup> mice were treated with 1-100 μg ICV doses (four times over 2 weeks). 1 day after the last dose, BMN 250 (100 μg doses) resulted in above-normal NAGLU activity levels, broad biodistribution, and uptake in all cell types, with NAGLU predominantly localized to neurons in the <i>Naglu</i><sup><i>-/-</i></sup> mouse brain. This led to complete clearance of disease-specific HS and reduction of secondary lysosomal defects and neuropathology across various brain regions lasting for at least 28 days after the last dose. The substantial brain uptake of NAGLU attainable by this highest ICV dosage was required for nearly complete attenuation of disease-driven storage accumulations and neuropathology throughout the <i>Naglu</i><sup><i>-/-</i></sup> mouse brain.
Design and Realization of Management and Control System of Warehouse Based on VC6 (2014)

The designing and realizing methods of network communication of the management and monitoring system of automatic tridimensional warehouse and proposed,the the main characters and the way of communication program developing with VC6 are expounded based on industrial Ethernet communication.Use the the ARM2440 control panel, the system is divided into the automated warehouse management and control system functional modules.And the Ethernet-based communication model used between the supervisor computer and the ARM2440 is presented. The realization and utilization of the design are described. The information and software integration of the system are illuminated, too. The design has lower cost and achieves system integration of management and control of an AS/RS by common devices and technology.
Calibration of pressure-velocity probes using a progressive plane wave reference field and comparison with nominal calibration filters (2011)

A procedure for calibrating pressure-velocity (p-v) sound intensity probes using a progressive plane wave as reference field is presented here. The procedure has been checked for a microelectromechanical system technology-based Microflown(®) match-size probe by comparing the calibration results with the nominal correction curves available from the manufacturer. The reference field was generated along a wave guide by means of a dual cone loudspeaker supplying acoustic energy in the range 20 Hz-20 kHz through an impedance adaptor. Different from the current in-field procedures, the one proposed here allows the calibration of probes under test to be executed at once up to 10 kHz without any change in the experimental setup. After a detailed review of the general principles of calibration, the procedure has been finalized with three main stages: (a) determination of the full coherence calibration bandwidth of the probe, (b) comparison calibration of the probe built-in pressure microphone over the full coherence frequency range, and (c) relative calibration of the velocity sensor over the calibrated pressure one. Calibration results for the probe under test have been best fitted against the calibration filters modeled by the manufacturer and the direct comparison of the obtained data with the factory ones has been reported.
Automatic focusing of an optical system by extremum control (1967)

A method is described for automatically focusing a high-power optical microscope using an extremum controller. The principle used is that of maximising the amplitudes of certain high-frequency components of a signal derived by scanning the object. The optimum bandpass filter required in selecting these frequency components is derived on the basis of expected power spectra, and the reasons for deviating, somewhat from this optimum are explained on the basis of the statistical variations in harmonic amplitudes of practical objects. A practicable system employing a peak-holding controller is described, in which the average hunting excursion about focus is less than the normally accepted depth of focus of the optical system.
Automatic Segmentation and Measurement of Pleural Effusions on CT (2013)

Pleural effusion is an important biomarker for the diagnosis of many diseases. We develop an automated method to evaluate pleural effusion on CT scans, the measurement of which is prohibitively time consuming when performed manually. The method is based on parietal and visceral pleura extraction, active contour models, region growing, Bezier surface fitting, and deformable surface modeling. Twelve CT scans with three manual segmentations were used to validate the automatic segmentation method. The method was then applied on 91 additional scans for visual assessment. The segmentation method yielded a correlation coefficient of 0.97 and a Dice coefficient of 0.72±0.13 when compared to a professional manual segmentation. The visual assessment estimated 83% cases with negligible or small segmentation errors, 14% with medium errors, and 3% with large errors.
Control of directly connected energy storage in LVDC distribution network (2015)

The paper focuses on developing the control for directly connected battery energy storage system (BESS) in low voltage direct current (LVDC) distribution systems. The main purpose of the control is to provide essential connection/disconnection and charge/discharge functions. Additionally, due to direct connection, it is necessary to implement control functions for management of the power flows in the distribution network. Furthermore, the control system is capable of running the system as island and seamlessly disconnecting and reconnecting to the feeding medium voltage (MV) network. Control system enables implementation of the Smart Grid (SG) functionalities and practically eliminates the interruptions seen by the electricity end-users.
Darstellung und Struktur von Na4GeSe4 · 14H2O (1976)

Abstract Selenogermanate(IV) können unter Luftabschluß aus wäßriger Lösung rein dargestellt werden. Das bei hohen pH‐Werten isolierte farblose Na 4 GeSe 4 · 14 H 2 O wurde röntgenstrukturanalytisch und schwingungsspektroskopisch charakterisiert. Es kristallisiert (Raumgruppe C2) mit a = 12,862, b = 11,205, c = 8,141 Å, β = 107,40° und enthält isolierte tetraedrische GeSe 4 4− ‐Ionen (GeSe 2,350 Å), die über Se…HO‐Brücken (Se…O 3,275 bis 3,634 Å) mit den hydratisierten Kationen im Kontakt stehen. Die Valenzschwingungen des GeSe 4 4− ‐Ions werden bei 211 (ν 1 ) und 280 cm −1 (ν 3 ) beobachtet. Die Valenzkraftkonstante beträgt etwa 1,90 mdyn/Å.
Roles of dopamine and serotonin in larval attachment of the barnacle,Balanus amphitrite (1999)

In order to clarify the roles of neurotransmitters including serotonin and dopamine in larval settlement (attachment) and metamorphosis of the barnacle Balanus amphitrite, the effects of lisuride, which acts as both a serotonin agonist/antagonist and a dopamine agonist, were examined. Lisuride did not induce larval attachment and metamorphosis; however, it promoted only larval behavior of searching for attachment sites without actual attachment to substrata which lasted for 5 to 6 days in a dose-dependent manner. Further evidence was obtained with a range of agonists/antagonists; serotonin agonists promoted the attachment, while serotonin antagonists inhibited it. Similarly, dopamine agonists inhibited the attachment. Furthermore, mixtures of serotonin and dopamine showed similar effects to those of lisuride. These results suggested that the promotion effect on larval searching behavior was derived from a combination of activities of serotonin and dopamine. Moreover, both serotonin and dopamine were detected in cyprids by HPLC. Thus, larval attachment process is regulated by both serotonin and dopamine neurons in this species. J. Exp. Zool. 284:746-758, 1999. Copyright 1999 Wiley-Liss, Inc.
A Simple and Rapid Method for Isolation of Retinal S Antigen (1982)

A procedure is described for the rapid isolation of S antigen from retinal tissue, by salt precipitation, gel filtration and final purification by adsorption chromatography on hydroxyapatite-agarose. The method yielded a highly purified material and allowed an excellent preservation of the antigenicity, as shown by high pathogenic activity in induction of experimental autoimmune uveoretinitis.
Developmental Changes in the Anatomy of the Sugarcane Stem in Relation to Phloem Unloading and Sucrose Storage (1992)

Abstract Transverse sections of immature and mature sugarcane internodes were investigated anatomically with white and fluorescence light microscopy. The pattern of lignification and suberization was tested histo‐chemically. Lignification began in the xylem of vascular bundles and progressed through the sclerenchymatic bundle sheath into the storage parenchyma. Suberization began in parenchyma cells adjacent to vascular bundle sheaths and spread to the storage parenchyma and outer sheath cells. In mature internodes most of the storage parenchyma was lignified and suberized to a significant degree, except in portions of walls of isolated cells. The pattern of increasing lignification and suberization in maturing internodes more or less paralleled an increase of sucrose in stem tissue. In mature internodes having a high sucrose concentration, the vascular tissue was surrounded by thick‐walled, lignified and suberized sclerenchyma cells. The apoplastic tracer dyes triso‐dium 3‐hydroxy‐5,8,10‐pyrenetrisulfonate (PTS) and amido black 10 B, fed into cut ends of the stalk, wereconfined to the vascular bundles in all internodes above the one that was cut — with no dye apparently in storage parenchyma tissue. Thus both structural and experimental evidence is consistent with vascular tissue being increasingly isolated from the storage parenchyma as maturation of the tissue proceeds. We conclude that in mature internodes the pathway for sugars from the phloem to the storage parenchyma is symplastic. The data suggest that an increasingly greater role for a symplastic pathway of sugar transfer occurs as the tissue undergoes lignification/suberization.
The prognostic value of histopathological grading systems in oral squamous cell carcinomas (2015)

A significant association between the BD model and outcome of OSCC patients was observed, indicating this new histopathological grading system as a possible prognostic tool.
High‐Throughput Optimization of Photochemical Reactions using Segmented‐Flow Nanoelectrospray ‐ Ionization Mass Spectrometry** (2023)

Within the realm of drug discovery, high-throughput experimentation techniques enable the rapid optimization of reactions and expedited generation of drug compound libraries for biological and pharmacokinetic evaluation. Herein we report the development of a segmented flow mass spectrometry-based platform to enable the rapid exploration of photoredox reactions for early-stage drug discovery. Specifically, microwell plate-based photochemical reaction screens were reformatted to segmented flow format to enable delivery to nanoelectrospray ionization-mass spectrometry analysis. This approach was demonstrated for the late-stage modification of complex drug scaffolds, as well as the subsequent structure-activity relationship evaluation of synthesized analogs. This technology is anticipated to expand the robust capabilities of photoredox catalysis in drug discovery by enabling high-throughput library diversification.
Characterization of Multiple Line Interconnection Structures from Time Domain Measurements (1993)

An experimental method for the time domain characterization of a class of nonuniformly coupled interconnection lines is presented. Electrical circuit models for constant velocity structures are extracted from time domain reflection (TDR) measurements. >
Interlayer exchange in (Ga,Mn)As/(Al,Ga)As/(Ga,Mn)As semiconducting ferromagnet/nonmagnet/ferromagnet trilayer structures (1998)

Magnetic properties of all-semiconductor (Ga,Mn)As/(Al,Ga)As/(Ga,Mn)As trilayer structures are studied. The interactions between the two ferromagnetic (Ga,Mn)As layers are investigated by magnetotransport measurements in a number of samples with different GaAs thickness or with different Al content in the intermediary nonmagnetic (Al,Ga)As layer. The results indicate that carriers present in the nonmagnetic layer mediate the coupling between the two ferromagnetic layers.
Atmospheric Pressure CO2 Pulsed Laser with Semiconducting Plastic Electrodes (1972)

The characteristics of a TEA laser with a new type of electrode have been investigated in a preliminary study. The method used consisted of replacing the discrete discharge elements by smooth semiconducting plastic electrodes which allow good control of the discharge, greater injection of CO 2 , and greater excitation cross section than the discrete discharge elements, and produce a plasma with good optical properties.
Gap Filling Strategies for 3-D-FBP Reconstructions of High-Resolution Research Tomograph Scans (2008)

The High-Resolution Research Tomograph (HRRT) is a dedicated human brain positron emission tomography scanner. Currently available iterative reconstruction algorithms show bias due to nonnegativity constraints. Consequently, implementation of 3-D filtered backprojection (3-D-FBP) is of interest. To apply 3-D-FBP all missing data including those due to gaps between detector heads need to be estimated. The aim of this study was to evaluate various gap filling strategies for 3-D-FBP reconstructions of HRRT data, such as linear and bilinear interpolation or constraint Fourier space gap filling (confosp). Furthermore, missing planes were estimated using segment 0 image data only (noniterative) or by using reconstructed images based on all previous segments (iterative method). Use of bilinear interpolation showed worst correspondence between reconstructed and true activity concentration, especially for small structures. Moreover, phantom data indicated that use of linear interpolation resulted in artifacts in planes located near the edge of the field-of-view. Use of confosp did not show these artifacts. Iterative estimations of the missing planes for |segments| 0 improved image quality at the cost of more computation time. Therefore, use of confosp for filling sinogram gaps with both iterative and noniterative estimation of missing planes are recommended for quantitative 3-D-FBP of HRRT studies.