gebhart/patrepeval huggingface dataset:
Algorithms in the Diagnosis and Management of Exotic Diseases. XVIII. Acute Viral Hepatitis (1977)

Journal Article Algorithms in the Diagnosis and Management of Exotic Diseases. XVIII. Acute Viral Hepatitis Get access D. P. Stevens, D. P. Stevens From the Divisions of Geographic Medicine and Gastroenterology, Department of Medicine, Case Western Reserve University and Uniuersity Hospital, Cleveland, Ohio Search for other works by this author on: Oxford Academic PubMed Google Scholar K. S. Warren, K. S. Warren From the Divisions of Geographic Medicine and Gastroenterology, Department of Medicine, Case Western Reserve University and Uniuersity Hospital, Cleveland, Ohio Search for other works by this author on: Oxford Academic PubMed Google Scholar A. A. F. Mahmoud A. A. F. Mahmoud From the Divisions of Geographic Medicine and Gastroenterology, Department of Medicine, Case Western Reserve University and Uniuersity Hospital, Cleveland, Ohio Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 135, Issue 1, January 1977, Pages 126–130, https://doi.org/10.1093/infdis/135.1.126 Published: 01 January 1977
Supplementary Table S2 from Metastatic Tumor-in-a-Dish, a Novel Multicellular Organoid to Study Lung Colonization and Predict Therapeutic Response (2023)

&lt;p&gt;TiD cultures better represent the response of cancer cell lines to chemotherapy than 2D cultures.&lt;/p&gt;
Fatal Spotted Fever Rickettsiosis, Kenya (2004)

We report a fatal case of rickettsiosis in a woman from the United States living in Kenya, who had a history of tick exposure. Immunohistochemical staining of skin, kidney, and liver demonstrated spotted fever group rickettsiae. The clinical findings, severity, and fatal outcome are most consistent with Rickettsia conorii infection.
Statistical Considerations for Quantitative Proteomics Data (2012)

A quantitative proteomics study employing SILAC technology was carried out to identify protein expression level differences between drug resistant and sensitive HeLa cells. Data analysis was performed with the aid of a newly developed SILAC dataset analysis program named SILACtor. Results are presented with an emphasis on the statistical considerations taken into account for study design and data interpretation.
Multiplex single‐cell quantification of rare RNA transcripts from protoplasts in a model plant system (2017)

Here we demonstrate multiplex and simultaneous detection of four different rare RNA species from plant, Arabidopsis thaliana, using surface-enhanced Raman spectroscopy (SERS) and gold nanoprobes at single-cell resolution. We show the applicability of nanoparticle-based Raman spectroscopic sensor to study intracellular RNA copies. First, we demonstrate that gold-nanoparticles decorated with Raman probes and carrying specific nucleic acid probe sequences can be uptaken by the protoplasts. We confirm the internalization of gold nanoprobes by transmission electron microscopy, inductively-coupled plasma-mass spectrometry and fluorescence imaging. Second, we show the utility of a SERS platform to monitor individual alternatively spliced (AS) variants and miRNA copies within single cells. Finally, the distinctive spectral features of Raman-active dyes were exploited for multiplex analysis of AtPTB2, AtDCL2, miR156a and miR172a. Furthermore, single-cell studies were validated by in vitro quantification and evaluation of nanotoxicity of gold probes. Raman tag functionalized gold nanosensors yielded an approach for the tracking of rare RNAs within the protoplasts. The SERS-based approach for quantification of RNAs has the capability to be a highly sensitive, accurate and discerning method for single-cell studies including AS variants quantification and rare miRNA detection in specific plant species.
The local lymph node assay and skin sensitization: a cut‐down screen to reduce animal requirements? (2006)

The local lymph node assay (LLNA), an alternative approach to skin-sensitizing testing, has made a significant contribution to animal welfare by permitting a reduction and refinement of animal use. Although there is clearly an aspiration to eliminate the use of animals in such tests, it is appropriate also to consider other opportunities for refinement and reduction of animal use. We have therefore explored the use of a modified version of the LLNA for screening purposes when there is a need to evaluate the sensitizing activity of a large number of chemicals, as will be the case under the auspices of registration, evaluation and authorization of chemicals (REACH). Using an existing LLNA database of 211 chemicals, we have examined whether a cut-down assay comprising a single high-dose group and a concurrent vehicle control would provide a realistic approach for screening chemicals for sensitizing potential. The analyses reported here suggest this is the case. We speculate that the animal welfare benefits may be enhanced further by reducing the number of animals per experimental group. However, a detailed evaluation will be necessary to provide reassurance that a reduction in group size would provide adequate sensitivity across a range of skin sensitization potencies.
Cognitive load of navigating without vision when guided by virtual sound versus spatial language. (2006)

A vibrotactile N-back task was used to generate cognitive load while participants were guided along virtual paths without vision. As participants stepped in place, they moved along a virtual path of linear segments. Information was provided en route about the direction of the next turning point, by spatial language ("left," "right," or "straight") or virtual sound (i.e., the perceived azimuth of the sound indicated the target direction). The authors hypothesized that virtual sound, being processed at direct perceptual levels, would have lower load than even simple language commands, which require cognitive mediation. As predicted, whereas the guidance modes did not differ significantly in the no-load condition, participants showed shorter distance traveled and less time to complete a path when performing the N-back task while navigating with virtual sound as guidance. Virtual sound also produced better N-back performance than spatial language. By indicating the superiority of virtual sound for guidance when cognitive load is present, as is characteristic of everyday navigation, these results have implications for guidance systems for the visually impaired and others.
The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC techniques. (1998)

The EnVision++ is an easy to use system, which avoids the possibility of disturbing endogenous biotin and lowers the cost per test by increasing the dilutions of the primary antibodies. Being a two step procedure, it reduces both the assay time and the workload.
Macromol. Rapid Commun. 19/2009 (2009)

Front Cover: Electrohydrodynamic co-jetting can result in microfibers with defined compartments. Here, the selective surface modification of bicompartmental microfibers is reported. This novel technology has the potential to address a so-far unmet requirement of tissue engineering: how to pattern cells in a three-dimensional scaffold framework. Further details can be found in the article by S. Mandal, S. Bhaskar, and J. Lahann* on page 1638.
Effect of substrate-induced strain on the structural, electrical, and optical properties of polycrystalline ZnO thin films (2004)

The effect of substrate-induced strain in polycrystalline ZnO thin films on different substrate, e.g., GaN epilayer, sapphire (0001), quartz glass, Si(111)∕SiO2, and glass deposited by sol-gel process, has been investigated by x-ray diffraction, scanning electron microscope, electrical resistivity, and photoluminescence measurements. A strong dependence of orientation, crystallite size, and electrical resistivity upon the substrate-induced strain along the c axis has been found. The results of structural and morphological studies indicate that relatively larger tensile strain exists in ZnO deposited on sapphire and glass, while a smaller compressive strain appears in film deposited on GaN and the strain is relaxed in larger crystallite size. The electrical resistivity of the films increases exponentially with increasing strain. The excitonic peak positions are found to shift slightly towards lower energy side with increasing strain. The analysis shows that GaN being a closely lattice-matched substrate produces ZnO films of better crystallinity with a lower resistivity.
Quantitative patterns of motor cortex proteinopathy across ALS genotypes (2020)

Degeneration of the primary motor cortex is a defining feature of amyotrophic lateral sclerosis (ALS), which is associated with the accumulation of microscopic protein aggregates in neurons and glia. However, little is known about the quantitative burden and pattern of motor cortex proteinopathies across ALS genotypes. We combined quantitative digital image analysis with multi-level generalized linear modelling in an independent cohort of 82 ALS cases to explore the relationship between genotype, total proteinopathy load and cellular vulnerability to aggregate formation. Primary motor cortex phosphorylated (p)TDP-43 burden and microglial activation were more severe in sporadic ALS-TDP disease than C9-ALS. Oligodendroglial pTDP-43 pathology was a defining feature of ALS-TDP in sporadic ALS, C9-ALS and ALS with OPTN, HNRNPA1 or TARDBP mutations. ALS-FUS and ALS-SOD1 showed less cortical proteinopathy in relation to spinal cord pathology than ALS-TDP, where pathology was more evenly spread across the motor cortex-spinal cord axis. Neuronal pTDP-43 aggregates were rare in GAD67+ and Parvalbumin+ inhibitory interneurons, consistent with predominant accumulation in excitatory neurons. Finally, we show that cortical microglia, but not astrocytes, contain pTDP-43. Our findings suggest divergent quantitative, genotype-specific vulnerability of the ALS primary motor cortex to proteinopathies, which may have implications for our understanding of disease pathogenesis and the development of genotype-specific therapies.
Electrospun Carbon felt-bare and modified with Gold nanoparticles-as electrode materials for biofuel cells (2015)

International audience
Structure of bovine carbonic anhydrase II at 1.95 Å resolution (2004)

Carbonic anhydrase (CA) is a zinc-containing enzyme that catalyzes the reversible hydration of CO2 to HCO3-. In eukaryotes, the enzyme plays a role in various physiological functions, including interconversion between CO2 and HCO3- in intermediary metabolism, facilitated diffusion of CO2, pH homeostasis and ion transport. The structure of bovine carbonic anhydrase II (BCA II) has been determined by molecular replacement and refined to 1.95 A resolution by simulated-annealing and individual B-factor refinement. The final R factor for the BCA II structure was 19.4%. BCA II has a C-terminal knot structure similar to that observed in human CA II. It contains one zinc ion in the active site coordinated to three histidines and one putative water molecule in a tetrahedral geometry. The structure of BCA II reveals a probable alternative proton-wire pathway that differs from that of HCA II.
A generalized inverse Nyquist stability criterion (1977)

In this paper the inverse Nyquist stability criterion, for single-input single-output linear feedback sy3tems, is generalized to the multi variable case. This is based on an association of a transfer function matrix with a set of inverse Nyquist diagrams, which will be termed the inverse characteristic gain loci. A simple example is given to illustrate the stability tests, and also to show how the inverse characteristic gain loci may be used in feedback system design
A Comparison of Analog and Digital Controls for Rotor Dynamic Vibration Reduction Through Active Magnetic Bearings (1991)

Active magnetic bearings are implemented using analog and digital controllers to achieve vibration reduction for multimass flexible rotors. Various models are developed for the rotor-bearing system, and the first three critical speeds (resonant frequencies) are shown to be unaffected by inclusion of the higher order shaft dynamics in the model. Higher order rotor-bearing models reveal the presence of “shaft modes,” the excitation of which is a function of the position of the magnetic bearing proximity probe (these modes are effectively damped by the flexible coupling employed in the test apparatus). Rotor dynamic behavior is investigated for various analog and digital controllers. Rotor response in the presence of proportional-derivative control is similar for both analog and digital control. Higher order digital control algorithms (second derivative and integral) affect the rotor response in a frequency dependent manner: Second derivative feedback is effective at reducing third mode vibration, and integral feedback, while rejecting any steady-state rotor position error, slightly accentuates the vibration at the first critical speed. Increasing the sampling rate of the digital controller has a similar effect to increasing the amount of second derivative feedback employed.
Arthroscopic Removal of Intra-articular Bone Fragment in Patient with Traumatic Posterior Fracture-dislocation of Hip Joint. A Case Report. (1999)

A twenty one-year old man was hit by a car and his left hip joint was dislocated posteriorly.After manual reduction, a small bone fragment was found between the joint space. Arthroscopic removal of the fragment was carried out successfully. The osteochondral fragment was 4cm long, 1cm wide, and 0.5cm thick, and derived from the posterior rim of the acetabulum. Full weight-bearing was permitted one week after the operation, and his hip function was recovered completely at a follow-up of four months post-operatively. Arthroscopic surgery proved to be less invasive and more useful than open surgery for the extraction of free bodies in the hip joint.
916. Sustained Whole-Body Functional Rescue by Systemic Delivery of AAV8 Vectors in Heart Failure and Muscular Dystrophy Hamsters (2005)

The success of muscular dystrophy gene therapy requires widespread and stable gene delivery with minimal invasiveness. Using the delta-sarcoglycan (|[delta]|SG)-deficient hamster (TO-2), a congestive heart failure and muscular dystrophy hamster model, here we show that a single injection of double-stranded adeno-associated virus serotype 8 vector carrying human delta-sarcoglycan gene (dsAAV8-|[delta]|SG), without the need of any physical or pharmaceutical interventions, achieved nearly complete gene transfer and tissue-specific expression in the heart and skeletal muscles of the diseases hamsters.
The V-Y Advancement Flap Is Equivalent to the Mustardé Flap for Ectropion Prevention in the Reconstruction of Moderate-Size Lid-Cheek Junction Defects (2012)

Therapeutic, III.
223 Practical application of Twin-roll type slag indirect cooling equipment (2006)

JFE developed a new slag cooling method suitable for molten slag from the ash vitrification process using an electric-resistance furnace. The system consists of twin drum type copper rolls cooled indirectly by coolant flowing inside. Slag is poured between the turning twin rolls and solidified. This paper describes the practical application of Twin-roll type slag indirect cooling equipment. The operating result shows that this method solves the conventional problems of space and water treatment.
Noisy feedback improves the Gaussian channel reliability function (2014)

For information transmission a discrete time channel with independent additive Gaussian noise is used. There is also a feedback channel with independent additive Gaussian noise, and the transmitter observes without delay all outputs of the forward channel via that noisy feedback channel. Transmission of nonexponential number of messages is considered and the achievable decoding error exponent for such a combination of channels is investigated. Transmission/decoding method used in the paper generalizes and strengthens the earlier method used by authors for BSC and Gaussian channel. It allows to improve essentially earlier results. In particular, for small feedback noise, it allows to gain 33.3% (instead of 23.6% in earlier papers).
Biophysical Properties of Purified Morphologic Forms of Hepatitis B Antigen (1974)

The morphological forms of hepatitis B antigen (HBsAg) present in an HBsAg-positive plasma (15–25 nm spheres, filaments 20–22 nm in diameter and 80–300 nm in length, and 42 nm Dane particles) were isolated and characterized biophysically. The HBsAg in this plasma was fractionated into five distinct populations, containing predominately 15–19 nm spheres, 20–22 nm spheres, 20 × 300 nm filaments, a mixture containing 23–25 nm spheres and rare filaments, and 42 nm Dane particles, respectively. These populations were labeled with 125I and electrofocused in a pH ampholyte gradient. Each population had a distinct isoelectric pH with values ranging from 3.65 to 4.70. Sodium dodecyl sulfate polyacrylamide gel electrophoretic analysis of each of the above labeled populations revealed the presence of 7, 10, 9, 5, and 9 polypeptides, respectively. The population containing predominately 23–25 nm spheres failed to react with antibody specific for HBsAg and probably was composed of inner cores of the Dane particles.
Lossless Source Coding for a Heegard-Berger Problem with Two Sources and Degraded Reconstruction sets (2015)

International audience
FOXO1 Competes with Carbohydrate Response Element-binding Protein (ChREBP) and Inhibits Thioredoxin-interacting Protein (TXNIP) Transcription in Pancreatic Beta Cells (2013)

Thioredoxin-interacting protein (TXNIP) has emerged as an important factor in pancreatic beta cell biology, and tight regulation of TXNIP levels is necessary for beta cell survival. However, the mechanisms regulating TXNIP expression have only started to be elucidated. The forkhead boxO1 transcription factor (FOXO1) has been reported to up-regulate TXNIP expression in neurons and endothelial cells but to down-regulate TXNIP in liver, and the effects on beta cells have remained unknown. We now have found that FOXO1 binds to the TXNIP promoter in vivo in human islets and INS-1 beta cells and significantly decreases TXNIP expression. TXNIP promoter deletion analyses revealed that an E-box motif conferring carbohydrate response element-binding protein (ChREBP)-mediated, glucose-induced TXNIP expression is necessary and sufficient for this effect, and electromobility shift assays confirmed FOXO1 binding to this site. Moreover, FOXO1 blocked glucose-induced TXNIP expression and reduced glucose-induced ChREBP binding at the TXNIP promoter without affecting ChREBP expression or nuclear localization, suggesting that FOXO1 may compete with ChREBP for binding to the TXNIP promoter. In fact, a FOXO1 DNA-binding mutant (FOXO1-H215R) failed to inhibit TXNIP transcription, and the effects were not restricted to TXNIP as FOXO1 also inhibited transcription of other ChREBP target genes such as liver pyruvate kinase. Together, these results demonstrate that FOXO1 inhibits beta cell TXNIP transcription and suggest that FOXO1 confers this inhibition by interfering with ChREBP DNA binding at target gene promoters. Our findings thereby reveal a novel gene regulatory mechanism and a previously unappreciated cross-talk between FOXO1 and ChREBP, two major metabolic signaling pathways.
Myocardial Relaxin Counteracts Hypertrophy in Hypertensive Rats (2005)

Current findings in male relaxin knockout mice indicate antifibrotic and positive lusitropic actions of relaxin on the myocardium. We investigated in 12-month-old male spontaneously hypertensive rats (SHR) and age-matched male Wistar-Kyoto rats (WKY) whether RLX shows antihypertrophic actions as well. SHR showed left heart hypertrophy and a selective elevation of left atrial and ventricular relaxin peptide which inversely correlated with the degree of hypertrophy. In adult rat cardiomyocytes, relaxin blunted phenylephrine-induced hypertrophy by inhibiting activated ERK-1/2 kinases. In conclusion, endogenous myocardial relaxin, upregulated in left heart hypertrophy, exerts antihypertrophic effects by inhibition of activated ERK-1/2 kinases.
Why HeLa cells do not produce interferon? (1977)

The investigation was carried out with HeLa cells which were not sensitive to antiviral action of human diploid cell interferon and do not produce interferon after induction with Newcastle disease virus. Identity of this line was confirmed by determination of stable genetic markers such as isoenzymes and histocompatibility antigens. On electrophoresis of lysed HeLa cells the following human red cell enzymes phenotypes were found: PGM (1-1), AP, EsD, and GTP. It was shown that on the surface of HeLa cells membranes HLA antigens are expressed: A28, BW35, A3. The results are in agreement with those which were obtained from Henrietta Lack's family study. Chromosome G-banding analysis revealed disproportion between the number of chromosomes connected with production and action of interferon and chromosomes which are supposed to control these processes.
V1390: Laparoscopic Partial Nephrectomy: Technical Considerations and Pitfalls in Complex Cases (2007)

You have accessJournal of UrologyVideo Session 5, Tuesday, May 22, 2007, 10:00 am - 12:00 pm1 Apr 2007V1390: Laparoscopic Partial Nephrectomy: Technical Considerations and Pitfalls in Complex Cases Erik P. Castle, Michael E. Woods, Raju Thomas, and Rodney Davis Erik P. CastleErik P. Castle More articles by this author , Michael E. WoodsMichael E. Woods More articles by this author , Raju ThomasRaju Thomas More articles by this author , and Rodney DavisRodney Davis More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)32188-8AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "V1390: Laparoscopic Partial Nephrectomy: Technical Considerations and Pitfalls in Complex Cases." The Journal of Urology, 177(4S), p. 458 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 177Issue 4SApril 2007Page: 458 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Erik P. Castle More articles by this author Michael E. Woods More articles by this author Raju Thomas More articles by this author Rodney Davis More articles by this author Expand All Advertisement PDF downloadLoading ...
The Catalytic Enantioselective, Protecting Group-Free Total Synthesis of (+)-Dichroanone (2006)

Herein we report the first enantioselective total synthesis of (+)-dichroanone, confirming the absolute configuration of the natural product. This protecting group-free route features the first application of our enantioselective Tsuji allylation in the context of a natural product total synthesis. Additionally, this 11-step preparation of the molecule from commercial material features a novel Kumada-aromatization strategy and a rapid sequence for the conversion of a phenol to a hydroxy-p-benzoquinone.
Acoustic point source scattering by a spherical elastic shell submerged beneath a free surface (1996)

The scattering of incident acoustic plane waves by a submerged spherical elastic shell near a free surface has previously been analyzed in detail using an exact, classical separation of variables solution. The problem is studied here again for cases where the incident waves are emitted from point sources at arbitrary positions in space. The result demonstrates that for a nearby point source, the scattered wave field is significantly different from that due to a plane incident wave, and that when the distance from the point source to the shell is more than 20 shell radii, the backscattered echo can be closely approximated by that of a plane incident wave. The scattered field due to an array of point sources can also be obtained by linear superpositions of the solution developed here.