gebhart/patrepeval huggingface dataset:
Rapid Analytical Method to Characterize the Freshness of Olive Oils Using Fluorescence Spectroscopy and Chemometric Algorithms (2020)

One of the most important issues in the field of quality assurance of olive oils is the detection of the freshness of olive oil. In this study, 400 nm laser-induced fluorescence spectroscopy was used with supervised and unsupervised multivariate analysis methods to develop a rapid method able to discriminate between freshly produced olive oils and oil that has been stored for a period of time ranging from 12 to 24 months. The fluorescence spectral data were firstly processed by the PCA. This method shows strong discrimination of the three oil classes using the first three components which present 96% of the total variability of the initial data, and then supervised classification models were constructed using the discriminant partial least square regression PLS-DA, support vector machine SVM, and linear discriminant analysis LDA. These methods show a high capacity in the classification of the three classes of olive oil. The validation of these classification models by external samples shows a high capacity of classification of the samples in their class with an accuracy of 100%. This study demonstrated the feasibility of the fluorescence spectroscopy fingerprint (routine technique) for the classification of olive oils according to their freshness and storage time.
Employing oxygen pulses to modulate Lachancea thermotolerans–Saccharomyces cerevisiae Chardonnay fermentations (2017)

Oxygen is sometimes deliberately introduced in winemaking at various stages to enhance yeast biomass formation and prevent stuck fermentation. However, there is limited information on how such interventions affect the dynamics of yeast populations. Our previous study in synthetic grape juice showed that oxygen supply enhances the persistence of Lachancea thermotolerans, Torulaspora delbrueckii and Metschnikowia pulcherrima. The three non-Saccharomyces yeasts showed differences in growth as a function of oxygen. The present study focused on evaluating the influence of short oxygen pulses on population dynamics and the aroma profile of Chardonnay wine inoculated with L. thermotolerans and Saccharomyces cerevisiae. The results confirmed a positive effect of oxygen on the relative performance of L. thermotolerans. The mixed culture fermentation with L. thermotolerans with S. cerevisiae developed a distinct aroma profile when compared to monoculture S. cerevisiae. Specifically, a high concentration of esters, medium chain fatty acids and higher alcohols was detected in the mixed culture fermentation. The data also showed that the longer persistence of L. thermotolerans due to addition of oxygen pulses influenced the formation of major volatile compounds such as ethyl acetate, ethyl butyrate, ethyl hexanoate, ethyl caprylate, ethyl caprate, ethyl-3-hydroxybutanoate, ethyl phenylacetate, propanol, isobutanol, butanol, isoamyl alcohol, hexanol, isobutyric acid, butyric acid, iso-valeric acid, hexanoic acid, octanoic acid, and decanoic acid.
55: HEMODYNAMIC RESPONSE TO IV ACETAMINOPHEN IN PEDIATRIC CARDIAC PATIENTS (2017)

Achuff, Barbara-Jo; Moffett, Brady; Acosta, Sebastian; Lasa, Javier; Checchia, Paul; Rusin, Craig Author Information
O-Linked N-Acetylglucosamine (O-GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1 (2017)

To study the regulation of colorectal adenocarcinoma progression by <i>O</i>-GlcNAc, we have focused on the <i>O</i>-GlcNAc-mediated epigenetic regulation of human colon cancer stem cells (CCSC). Xenograft tumors from colon tumor cells with <i>O</i>-linked <i>N</i>-acetylglucosamine transferase (OGT) knockdown grew significantly slower than those formed from control cells, indicating a reduced proliferation of tumor cells due to inhibition of OGT expression. Significant reduction of the CCSC population was observed in the tumor cells after OGT knockdown, whereas tumor cells treated with the <i>O</i>-GlcNAcase inhibitor showed an increased CCSC population, indicating that <i>O</i>-GlcNAc levels regulated the CCSC compartment. When grown in suspension, tumor cells with OGT knockdown showed a reduced ability to form tumorspheres, indicating a reduced self-renewal of CCSC due to reduced levels of <i>O</i>-GlcNAc. ChIP-sequencing experiments using an anti-<i>O</i>-GlcNAc antibody revealed significant chromatin enrichment of <i>O</i>-GlcNAc-modified proteins at the promoter of the transcription factor <i>MYBL1</i>, which was also characterized by the presence of H3K27me3. RNA-sequencing analysis showed an increased expression of <i>MYBL1</i> in tumor cells with OGT knockdown. Forced overexpression of MYBL1 led to a reduced population of CCSC and tumor growth <i>in vivo</i>, similar to the effects of OGT silencing. Moreover, two CpG islands near the transcription start site of <i>MYBL1</i> were identified, and <i>O</i>-GlcNAc levels regulated their methylation status. These results strongly argue that <i>O</i>-GlcNAc epigenetically regulates <i>MYBL1</i>, functioning similarly to H3K27me3. The aberrant CCSC compartment observed after modulating <i>O</i>-GlcNAc levels is therefore likely to result, at least in part, from the epigenetic regulation of <i>MYBL1</i> expression by <i>O</i>-GlcNAc, thereby significantly affecting tumor progression.
Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature Pig (2009)

Induced pluripotent stem cell (iPS) technology appears to be a general strategy to generate pluripotent stem cells from any given mammalian species. So far, iPS cells have been reported for mouse, human, rat, and monkey. These four species have also established embryonic stem cell (ESC) lines that serve as the gold standard for pluripotency comparisons. Attempts have been made to generate porcine ESC by various means without success. Here we report the successful generation of pluripotent stem cells from fibroblasts isolated from the Tibetan miniature pig using a modified iPS protocol. The resulting iPS cell lines more closely resemble human ESC than cells from other species, have normal karyotype, stain positive for alkaline phosphatase, express high levels of ESC-like markers (Nanog, Rex1, Lin28, and SSEA4), and can differentiate into teratomas composed of the three germ layers. Because porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutic applications of iPS in a large animal model.
Prothetischer Ersatz des Daumensattelgelenkes mit einer zementierten Kugelgelenkprothese (de la Caffinière) (2000)

We analysed the fate of 43 de la Caffinière prostheses implanted for arthrosis of the first carpometacarpal joint after a follow-up of 63 months (range 45 to 81). Ten prostheses failed. Further eight patients were lost for follow up. In the remaining 25 patients the rate of tilting of the cups (92%) was extremely high and the survival rate disappointing: 66% at 68 months. The prostheses were implanted in cancellous bone and the ball and socket reduces the moving axes of the normal joint to one center of rotation. These factors might explain the higher rate of loosening of the cups (28%), compared to 15% for the stems. In contrast, the clinical results were comparable with other operative procedures. The advantage is the brief postoperative rehabilitation period. Accordingly, this prosthesis can not be universally recommended and should be reserved for elderly patients, who must not undertake strenuous work.
Methods for determining Carbon dioxide production in soils (1932)

Many investigators have studied the organic matter in soils, in the attempt to determine its nature and composition, the rate and character of the decomposition which it undergoes, the products of decomposition, and especially the value of various kinds of organic materials which may be applied to soils, as indicated by decomposition studies.
Physically-based statistical analysis of nonlinear circuits through X-parameters (2019)

The active device X-parameters extracted from physics-based TCAD simulations are imported into the Keysight ADS environment following the techniques outlined in [1], leading to an efficient, yet physically sound, circuit-level nonlinear model, retaining a direct link with the device fabrication technology. In this work, the X parameter model is used to demonstrate that a fully statistical analysis of a nonlinear circuit as a function of technological spread of the active device can be efficiently carried out within ADS, with extremely reduced simulation time and excellent agreement with the original TCAD analysis. As a test case, we address the statistical analysis of a deep class AB amplifier as a function of the variations of both the active device doping and the circuit layout. The statistical distribution of the output power significantly depends on the power backoff and exhibits a marked skew, which cannot be fully addressed by standard sensitivity or linearized approaches. The effect of correlated or uncorrelated active device or layout variations is also investigated in a combined stage.
High-pulse-energy, linear optical parametric oscillator with narrow and symmetrical far field (2013)

A new method to obtain a narrow and symmetrical far field from a high-pulse-energy optical parametric oscillator (OPO) with a linear resonator has been tested. The OPO employs two identical nonlinear crystals that are cut for type II phase matching, rotated such that their walk-off planes are orthogonal, and separated by a broadband half-wave plate. The OPO has a simple geometry, can be double-pass pumped, is wavelength tunable and operates stably with high conversion efficiency. The method has been demonstrated in a KTP-based OPO pumped at 1064 nm and a BBO-based OPO pumped at 532 nm, with output pulse energies up to 60 mJ and 75 mJ, respectively.
p16INK4a expression is frequently decreased and associated with 9p21 loss of heterozygosity in sporadic melanoma (1998)

The product of the p16/INK4a/CDKN2/MTS1 tumor-suppressor gene acts as a negative cell cycle regulator by inhibiting G1 cyclin-dependent kinases that phosphorylate the retinoblastoma protein. p16 is inactivated in a wide range of human malignancies, including familial melanoma. However, its expression and function in sporadic melanoma has not been extensively investigated. We studied p16 expression in 62 archival melanomas and 30 archival nevi and lentigines by immunohistochemistry. Eighteen of 26 (69%) superficial spreading melanomas, 17 of 28 (61%) nodular melanomas, all of three lentigo maligna melanomas, and all of five melanoma metastases were found to harbor less than 10% p16-positive tumor cells. In contrast, only six of 24 (25%) nevi had less than 10% positive cells. No correlation between tumor thickness and loss of p16 expression was found. Using DNA from micro-dissected tumor and matched normal tissues, five of seven (71%) p16-negative melanoma cases had 9p21 loss of heterozygosity (LOH), and one of these 9p21 LOH cases had promoter region hypermethylation of the remaining p16 allele. These data demonstrate that partial or complete loss of p16 expression is prevalent in sporadic melanoma and is frequently associated with 9p21 LOH.
Proton-coupled electron transfer between [Ru(bpy)2(py)OH2]2+ and [Ru(bpy)2(py)O]2+. A solvent isotope effect (kH2O/kD2O) of 16.1 (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProton-coupled electron transfer between [Ru(bpy)2(py)OH2]2+ and [Ru(bpy)2(py)O]2+. A solvent isotope effect (kH2O/kD2O) of 16.1Robert A. Binstead, Bruce A. Moyer, George J. Samuels, and Thomas J. MeyerCite this: J. Am. Chem. Soc. 1981, 103, 10, 2897–2899Publication Date (Print):May 1, 1981Publication History Published online1 May 2002Published inissue 1 May 1981https://pubs.acs.org/doi/10.1021/ja00400a083https://doi.org/10.1021/ja00400a083research-articleACS PublicationsRequest reuse permissionsArticle Views1109Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Design of artificial cell–cell communication using gene and metabolic networks (2004)

Artificial transcriptional networks have been used to achieve novel, nonnative behavior in bacteria. Typically, these artificial circuits are isolated from cellular metabolism and are designed to function without intercellular communication. To attain concerted biological behavior in a population, synchronization through intercellular communication is highly desirable. Here we demonstrate the design and construction of a gene-metabolic circuit that uses a common metabolite to achieve tunable artificial cell-cell communication. This circuit uses a threshold concentration of acetate to induce gene expression by acetate kinase and part of the nitrogen-regulation two-component system. As one application of the cell-cell communication circuit we created an artificial quorum sensor. Engineering of carbon metabolism in Escherichia coli made acetate secretion proportional to cell density and independent of oxygen availability. In these cells the circuit induced gene expression in response to a threshold cell density. This threshold can be tuned effectively by controlling DeltapH over the cell membrane, which determines the partition of acetate between medium and cells. Mutagenesis of the enhancer sequence of the glnAp2 promoter produced variants of the circuit with changed sensitivity demonstrating tunability of the circuit by engineering of its components. The behavior of the circuit shows remarkable predictability based on a mathematical design model.
Embryonic Stem Cells (2007)

Stem cells are unique cell populations with the ability to choose between self-renewal and differentiation. Embryonic stem (ES) cells have the ability to form any fully differentiated cell of the body. To date, only three species of mammals have yielded long-term cultures of self-renewing ES cells- mice, monkeys, and humans. These cells have some special requirements to maintain their undifferentiated state in culture, e.g., presence of feeder cells, serum, or cytokines. Many scientific studies have tried to manipulate the growth and differentiation conditions with varied success. Studies of development in model systems, such as mice help our efforts to manipulate human stem cells in vitro. Data are now emerging that ES cells can be directed toward lineage-specific differentiation programs. On the basis of this property, it is likely that human ES cells will provide a useful differentiation culture system to study the mechanisms of human development. Recent advances in culturing ES cells and success in exploiting their pluripotency brings great hope for using human ES cell-based reparative therapy in future.
Molecular Cloning of a Monoclonal Anti-Tumor Antibody Specific for the Tn Antigen and Expression of an Active Single-Chain Fv Fragment (1997)

We report here the first amino acid sequence of an anti-Tn monoclonal antibody raised against human breast cancer cells and show that a single chain Fv fragment of this IgM retains the Tn-binding specificity as defined by functional assays with asialo-OSM and membrane extracts from MCF-7 cells. Sequence comparisons and molecular modeling of 83D4 indicate that the antibody combining site displays a cavity-like feature primarily defined by the CDR H1 and H2 loops. This pocket could accommodate a single Tn molecule, thus, suggesting a structural explanation for the predominant expression of a particular VH gene segment in a group of antibodies that recognize tumor-associated antigens arising from an aberrant O-glycosylation.
Evaluation of Organ Weights for Rodent and Non-Rodent Toxicity Studies: A Review of Regulatory Guidelines and a Survey of Current Practices (2007)

The Society of Toxicologic Pathology convened a working group to evaluate current practices regarding organ weights in toxicology studies. A survey was distributed to pharmaceutical, veterinary, chemical, food/nutritional and consumer product companies in Europe, North America, and Japan. Responses were compiled to identify organs routinely weighed for various study types in rodent and non-rodent species, compare methods of organ weighing, provide perspectives on the value of organ weights and identify the scientist(s) responsible for organ weight data interpretation. Data were evaluated as a whole as well as by industry type and geographic location. Regulatory guidance documents describing organ weighing practices are generally available, however, they differ somewhat dependent on industry type and regulatory agency. While questionnaire respondents unanimously stated that organ weights were a good screening tool to identify treatment-related effects, opinions varied as to which organ weights are most valuable. The liver, kidneys, and testes were commonly weighed and most often considered useful by most respondents. Other organs that break were commonly weighed included brain, adrenal glands, ovaries, thyroid glands, uterus, heart, and spleen. Lungs, lymph nodes, and other sex organs were weighed infrequently in routine studies, but were often weighed in specialized studies such as inhalation, immunotoxicity, and reproduction studies. Organ-to-body weight ratios were commonly calculated and were considered more useful when body weights were affected. Organ to brain weight ratios were calculated by most North American companies, but rarely according to respondents representing veterinary product or European companies. Statistical analyses were generally performed by most respondents. Pathologists performed interpretation of organ weight data for the majority of the industries.
Immunocastration of Farm Animals (2005)

In the farm animal species, the control of fertility and of sexual and aggressive behaviour are important factors of productivity. Traditionally, they have been reduced by surgical approaches. But, castration becomes unacceptable generating stress and decreasing animal efficiency and carcass quality. Therefore, alternative castration method such as immunocastration has been considered. This alternative method involves active immunisation against a hormone or factor, which is implicated in the cascade of events of the hypothalamic-pituitary-gonadal axis. Finally, a last procedure which consists in the application of recombinant DNA technologies to produce viral vector for hormone and other factors is under studies.
Efficient Control of the Rheological and Surface Properties of Surfactant Solutions Containing C8–C18 Fatty Acids as Cosurfactants (2013)

Systematic experimental study is performed about the effects of chain length (varied between C8 and C18) and concentration of fatty acids (FAc), used as cosurfactants to the mixture of the anionic surfactant SLES and the zwitterionic surfactant CAPB. The following properties are studied: bulk viscosity of the concentrated solutions (10 wt % surfactants), dynamic and equilibrium surface tensions, surface modulus, and foam rheological properties for the diluted foaming solutions (0.5 wt % surfactants). The obtained results show that C8-C10 FAc induce formation of wormlike micelles in the concentrated surfactant solutions, which leads to transformation of these solutions into viscoelastic fluids with very high apparent viscosity. The same FAc shorten the characteristic adsorption time of the diluted solutions by more than 10 times. In contrast, C14-C18 FAc have small effect on the viscosity of the concentrated solutions but increase the surface modulus above 350 mN/m, which leads to higher friction inside sheared foams and to much smaller bubbles in the formed foams. The intermediate chain C12 FAc combines some of the properties seen with C10 FAc and other properties seen with C14 FAc. These results clearly demonstrate how appropriate cosurfactants can be used for efficient control of the rheological properties of concentrated surfactant solutions and of some important foam attributes, such as bubble size and foam rheology.
Split shell open pit design concept applied at De Beers Venetia Mine South Africa using the Whittle and Gemcom software (2001)

In todayi?½s high tech world, systems are getting bigger, faster, better. To remain at the leading edge, in any business, depends on accurate but flexible design thereby allowing operational managers to provide alternatives to improve their operations. The ultimate goal is to deliver to plan, with optimum economics. Mining is no different, with falling prices and escalating costs, improved margins become the focal objective. There are many ways to achieve this, ranging from operational efficiency improvements through production scenarios to planning initiatives. Of all, planning usually receives the least attention, yet it is the area of most influence on the NPV of the reserve. With computer technology, numerous concepts and iterations are possible thereby closing the gap between optimal and actual design. This paper discusses the application of the split shell design at the De Beers Venetia open pit in South Africa.
Chronic pneumonia with Pseudomonas aeruginosa and impaired alveolar fluid clearance (2005)

From these results, we observe an adequate initial alveolar response to increased permeability with an increase of DAFC. However, DAFC increase does not persist after the 5th day and remains unresponsive to stimulation. This impairment of DAFC may partly explain the higher susceptibility of chronically infected patients to subsequent lung injury.
Human Liver Stem Cell-Derived Microvesicles Inhibit Hepatoma Growth in SCID Mice by Delivering Antitumor MicroRNAs (2012)

Microvesicles (MVs) play a pivotal role in cell-to-cell communication. Recent studies demonstrated that MVs may transfer genetic information between cells. Here, we show that MVs derived from human adult liver stem cells (HLSC) may reprogram in vitro HepG2 hepatoma and primary hepatocellular carcinoma cells by inhibiting their growth and survival. In vivo intratumor administration of MVs induced regression of ectopic tumors developed in SCID mice. We suggest that the mechanism of action is related to the delivery of microRNAs (miRNAs) from HLSC-derived MVs (MV-HLSC) to tumor cells on the basis of the following evidence: (a) the rapid, CD29-mediated internalization of MV-HLSC in HepG2 and the inhibition of tumor cell growth after MV uptake; (b) the transfer by MV-HLSC of miRNAs with potential antitumor activity that was downregulated in HepG2 cells with respect to normal hepatocytes; (c) the abrogation of the MV-HLSC antitumor effect after MV pretreatment with RNase or generation of MVs depleted of miRNAs; (d) the relevance of selected miRNAs was proven by transfecting HepG2 with miRNA mimics. The antitumor effect of MV-HLSC was also observed in tumors other than liver such as lymphoblastoma and glioblastoma. These results suggest that the delivery of selected miRNAs by MVs derived from stem cells may inhibit tumor growth and stimulate apoptosis.