gebhart/patrepeval huggingface dataset:
A quantum-chemical study of phenol self-association of open and cyclic dimers and trimers (1984)

Itermolecular hydrogen bond in the open/linear and cyclic/closed dimers and trimers of phenol has been studied by means of the quantum-chemical PCILO method. Our calculations have shown that the open /linear associate is more stable in the case of the dimers. On the contrary, the cyclic/closed associate is more stable in the case of the trimers. Positive cooperativity has been observed by formation of both the open/linear and cyclic/closed trimers
A New Method for Studying Stress-Induced Microcracks in Concrete (1998)

The experimental technique described below makes it possible to preserve the compressive stress-induced microcracks in concrete as they exist under applied loads. The results can be used to better understand and quantify the general relationship between stress level and crack development, as well as the effect of confinement on crack behavior. Also, it allows observations into the way small cracks are formed and then propagate in concrete, thereby making the application of fracture mechanics to concrete more accurate. Cylindrical specimens of concrete were subjected to testing under uniaxial and confined compression utilizing special testing equipment designed and developed specifically for this experimental study. An alloy with a low melting point was used as a pore fluid. At a specific stress, this alloy was solidified to preserve the stress-induced microcracks as they exist under load. Scanning electron microscopy was employed to capture images from the cross sections of normal and high-strength concrete specimens.
Hyperthermia‐induced increase in potassium transport in Chinese hamster cells (1983)

Abstract The effect of hyperthermia (42°C) on K + transport has been examined by monitoring the uptake of 42 KCl by Chinese hamster (line CHO) cells at both 37° and 42°C as well as 37°C following a 30‐min treatment at 42°C. Early studies showed that CHO cells exhibit two components of uptake at both 37° and 42°C. Consequently, it was necessary to determine rate equations describing a three‐compartment system (i.e., the bathing medium and two cell‐associated compartments) and apply them to this system. Using the equations describing the behavior of the tracer in each compartment as well as the general solutions given by Robertson et al (1957), a method was developed for obtaining the two components of influx. The two transport rates were determined from equations arising from mathematical analysis and requiring only measurable quantities for their solutions. The results of this study showed that (1) both components of influx at 42°C are increased over influx at 37°C; (2) the increase is significant (by an average 68% for the rapid component and 55% for the slow component); and (3) the increase is due entirely to an increase in active transport. The increased active transport is both immediate and quickly reversible when cells are returned to 37°C; resumption of cell division is delayed for several additional hours, indicating that changes in potassium transport are not directly responsible for the prolonged delay in cell division. Rather, the results suggest that increased activity of the membrane pump may be the initial event in a series of events resulting from heat treatment.
Degradation of n-haloalkanes and alpha, omega-dihaloalkanes by wild-type and mutants of Acinetobacter sp. strain GJ70 (1987)

A 1,6-dichlorohexane-degrading strain of Acinetobacter sp. was isolated from activated sludge. The organism could grow with and quantitatively release halide from 1,6-dichlorohexane, 1,9-dichlorononane, 1-chloropentane, 1-chlorobutane, 1-bromopentane, ethylbromide, and 1-iodopropane. Crude extracts contained an inducible novel dehalogenase that liberated halide from the above compounds and also from 1,3-dichloropropane, 1,2-dibromoethane, and 2-bromoethanol. The latter two compounds were toxic suicide substrates for the organism at concentrations of 10 and 5 microM, respectively. Mutants resistant to 1,2-dibromoethane (3 mM) lacked dehalogenase activity and did not utilize haloalkanes for growth. Mutants resistant to both 1,2-dibromoethane (3 mM) and 2-bromoethanol (30 mM) could no longer oxidize or utilize alcohols and were capable of hydrolytic dehalogenation of 1,2-dibromoethane to ethylene glycol.
Response: Cys186-Cys209 disulfide–mutated tissue factor does not equal cryptic tissue factor: no impairment in decryption of disulfide mutated tissue factor (2010)

The primary focus of our recent report[1][1] is to examine whether Cys186-Cys209 disulfide–mutated tissue factor (TF) mimics cryptic TF and the importance of Cys186-Cys209 disulfide bond formation in TF decryption, 2 key issues that were not examined earlier.[2][2]

In our view, one should be
Technological, Biochemical and Microbiological Evaluation of DehydratedPleurotus ostreatus Powder for Nutraceutical Applications (2022)

Abstract: Mushroom-based products can serve as superior nutraceuticals in both preventing and alleviating human diseases. Dehydrated powder of Pleurotus ostreatus grown in Cuba has a rich nutritional composition and a large number of therapeutic properties. However, technological parameters (e.g. rheological), as well as biochemical and microbiological quality control standards for its use as a raw material in the manufacture of solid dosage forms with nutraceutical and biopharmaceutical applications have not been established yet. Objective: The study aims to determine the technological, biochemical and microbiological quality parameters of dehydrated Pleurotus ostreatus powder. Method: Rheological properties (Carr´s index, Hausner´s ratio, angle of repose and flow rate) were determined in seven batches of mushroom powder. Biochemical and microbiological analyses were performed using standard methods. Other physicochemical properties (mean particle size, pH and moisture) were also determined. Results: Technical parameters showed a mean particle size of 224.08±7.96 μm, Carr´s compressibility index (23.08-26.58%), Hausner´s ratio (1.30-1.36), angle of repose (36.6-41.90°) and flow rate (3.69-6.93 g cm-2 s-1), typical of materials with poor flowability. Biochemical analyses indicated significant nutraceutical composition: protein (25.0-30.4%), carbohydrate (45-60%), ash (6.1-9.8%), fiber (5-9%), β-D-Glucans (1.47-1.61%), iron (21-50 mg/100 g), zinc (3.8-8.0 mg/100 g), and polyphenols (123-160 mg/100 g). Moisture content was ≤3.50% and microbiological analyses were in agreement with ISO standards. Conclusion: Dehydrated P. ostreatus powder exhibits good nutraceutical composition and microbiological quality. Technological parameters evidenced poor flow properties, suggesting the need to include excipients to improve the rheological properties for applications as an active ingredient in solid dosage forms for health benefits.
Automated generation of VNF deployment rules using infrastructure affinity characterization (2016)

As the rollout of NFV in the Telecommunications domain gains momentum, methods that enable the automated and performant deployment of VNFs are required to support large scale deployments. This paper presents a framework that enables the automated characterization of VNFs and modelling of the relationships between resource types, quantities and achieved performance. A workload characterization approach is proposed to identify the most appropriate types of resources to be allocated to a VNF at deployment time. Machine learning modelling was used to identify the appropriate deployment configuration which meets required performance targets, such as network throughput. The proposed framework has been experimentally validated based on the optimization of a Traffic Classifier VNF deployment in an OpenStack cloud environment.
Utility of a rapid assay for Prostaglandin E-major urinary metabolite as a biomarker in pediatric ulcerative colitis (2023)

Abstract Prostaglandin E-major urinary metabolite (PGE-MUM) is a urinary biomarker reflecting ulcerative colitis (UC) activity. This prospective observational study aimed to evaluate the usefulness of PGE-MUM via rapid chemiluminescent enzyme immunoassay in detecting endoscopic remission (ER) and histologic remission (HR) in pediatric UC (6–16 years) in comparison with fecal calprotectin (FCP). ER and HR were defined as Mayo endoscopic score (MES) of 0 and Matts’ histological grades (Matts) of 1 or 2, respectively. A total of 104 UC and 39 functional gastrointestinal disorder (FGID) were analyzed. PGE-MUM levels were significantly higher in the UC group than in the FGID group ( P &lt; 0.001). FCP levels were significantly elevated in the group without ER and HR than in the group with ER and HR ( P &lt; 0.001 and P = 0.001), whereas PGE-MUM levels were significantly higher in the group without ER compared to the group with ER ( P &lt; 0.001). No significant differences were noted in the AUCs for PGE-MUM and FCP in detecting ER and HR. Although PGE-MUM was inferior to FCP for the detection of HR, it might have the potential for application as a biomarker of endoscopic activity in pediatric UC owing to its noninvasive and rapid method.
Total synthesis of sesquicarene (1969)

R. M. Coates and R. M. Freidinger, J. Chem. Soc. D, 1969, 871 DOI: 10.1039/C29690000871
Resilient Modulus Testing for Pavement Components (2003)

Description Nineteen peer-reviewed papers address the latest issues affecting the resilient modulus test and use of the test results. Resilient modulus indicates the stiffness of a soil under controlled confinement conditions and repeated loading. The test is intended to simulate the stress conditions that occur in the base and subgrade of a pavement system. Papers cover: • Repeatability of the test by testing replicated test specimens under the same conditions. • Background of test startup and QC procedures • Review of the LTPP test program • Effect of water content and pore water pressure buildup on the resilient modulus of unsaturated and saturated cohesive soils • Importance of the value of resilient modulus on required pavement thickness This book is a valuable resource for highway engineers, geotechnical engineers, laboratory personnel who conduct pavement tests, pavement designers, and foundation engineers.
Precipitation of copper in ferrite: Prediction of the strengthening kinetics (2004)

A physically based model to predict the strengthening kinetics induced by copper precipitation in the system Fe-Cu has been developed. The effects of aging time, temperature and composition are integrated in the model. The results of the calculations are in good agreement with the experimental observations.
Interacciones entre las poblaciones de acaros tetraniquidos y fitoseidos y el desherbado quimico de los vinedos en Francia. Resultados de campo (2a Parte) (1992)

Extraordinario Nutri fitos
An Analysis of Creep Crack Growth of Interface Cracks in Layered/Graded Materials (2008)

In this study, the growth behavior of interface cracks in bimaterials and in layered materials resulting from the creep cavitation was studied. The growth model includes the effects of material deposition resulting from the growth of creep cavities on the crack tip stress fields. The results indicate that in layered materials under identical applied loading, the location of the interface crack strongly influence the amplitude of the stress field (C*) at steady-state. Due to large variation in the distribution of the stresses ahead of the interface cracks at creep regime, depending upon the crack location, the creep crack growth rates will be significantly different from each other under identical loading for a given layered material.
The effect of caffeine on diaphragmatic muscle force in normal hamsters. (1982)

The effect of caffeine on the tension generated by fresh and fatigued diaphragmatic muscle was examined in 32 Syrian hamsters. Studies were performed in vitro on innervated diaphragmatic muscle strips activated by electrical stimuli applied either directly or via the phrenic nerve. The degree of activation of the muscle was varied by altering the frequency of stimulation. When the stimulus frequency was low (less than 50 Hz), caffeine (1 mM and 5 mM) augmented the tension generated by fresh muscle in response to both phrenic nerve and direct stimulation in a dose-dependent fashion. At high stimulus frequencies (greater than 50 Hz), however, the response to caffeine was different in directly activated and phrenic-nerve-activated muscles. Caffeine augmented tension production at all stimulus frequencies in directly activated muscles, but decreased the tension developed in response to phrenic stimulation. The increase in tension elicited by caffeine during direct stimulation was relatively smaller at high compared with at low stimulus frequencies. Caffeine augmented peak tension by increasing the rate of tension development and prolonging the period during which tension increased. Similar effects were observed in muscle strips fatigued by prolonged activity. We conclude that caffeine augments tension production in both fresh and fatigued diaphragmatic muscle by a direct effect on the processes that activate contraction and that this effect is greatest when the muscle is minimally activated (low stimulus frequencies). Caffeine, however, may adversely affect muscle tension by impairing neuromuscular transmission.
Substitution of two histidine residues in YadA protein of Yersinia enterocolitica abrogates collagen binding, cell adherence and mouse virulence (1995)

The plasmid-encoded surface protein YadA of Yersinia enterocolitica mediates binding to diverse extracellular matrix (ECM) proteins, adherence to epithelial cell lines, resistance to complement lysis, autoagglutination, and is required for mouse virulence. Using site-directed mutagenesis we attempted to analyse the relationship between structural domains and functions of YadA. In a first approach we could abrogate collagen binding by chemical modification of histidyl residues of YadA protein. This result prompted us to substitute histidyl residues (His) of conserved regions of YadA protein of Y. enterocolitica O8 by tyrosine residues using site-directed mutagenesis. Substitution of His-156 and His-159 (YadA-2 mutant) resulted in abrogation of binding to ECM proteins, of cell adherence, and in reduction of mouse virulence, whereas autoagglutination, serum complement resistance and oligomer formation remained unaffected. A striking result was obtained from the orogastric mouse-infection model: the YadA-2 mutant retained the ability to colonize the small intestine and to invade and multiply within the Peyer's patches but was impaired in colonizing mesenteric lymph nodes and spleen in comparison to the wild-type strain.
PHYSICAL DEVELOPMENT OF PRESCHOOL CHILDREN OF RURAL AREA OF KRASNODAR KRAI (2017)

Aim. The aim of the work was evaluation of physical development of preschool children of rural area of Krasnodar Krai. Materials and methods. Research was conducted on the base of six municipal preschool establishments of rural area of Krasnodar Krai. 538 preschool children (282 boys and 256 girls) aged from 3 to 7 years took part in it. Their physical development was estimated by means of sigma deviation method on the basis of height and bodyweight, a result was compared to the evaluation tables of physical development of preschool children ofKrasnodar. Results. It was shown that the majority of children evaluated are normally physically developed (76,02±1,84%, of which there were 196 girls (out of the total number of the evaluated girls 76,56±2,09%) and 213 boys (75,53±2,13% out of the total number of the evaluated boys), but the percent of children with disorders of physical development was rather high (23,98±1,84%). It has also been discovered, that a small percent of preschool children has a general physical developmental delay. Conclusion. The results of research demonstrated gender distinctions in the indexes of physical development of preschool children of rural area. A compromised physical development is observed in girls more often than in boys, that testifies that girls are in a high-risk group of health status deviations.
Splitting and lasing of whispering gallery modes in quantum dot micropillars (2011)

Whispering gallery mode splitting in high quality-factor InAs quantum dot micropllars is observed and attributed to resonant scattering from the dots themselves. Low-threshold multimode lasing with beta-factors approaching unity is demonstrated.
Preliminary observations on excretion of 37Ar from man following whole-body neutron activation--an indicator of total-body calcium. (1975)

Measurements of exhaled 37-Ar produced by total-body neutron irradiation of 40-Ca were used to determine total-body calcium in 16 human subjects. There was a good correlation between body calcium using the 30-min postirradiation breath sample of 37-Ar and body calcium determined by measurement of 49-Ca.
[The mechanisms of recombination in human immunodeficiency virus]. (2006)

The importance of recombination in retroviral evolution has been acknowledged for several decades. After the identification of HIV as the etiological agent of AIDS, it was suspected that recombination could also play a central role in the evolution of this virus. However, only recently extensive epidemiological studies of HIV infections worldwide have provided an estimate for the occurrence of recombination in vivo, unveiling recombination frequencies that dwarf those initially expected. Nowadays, recombination is regarded as an integral part of the infectious cycle of this retrovirus, demonstrating its major role in HIV evolution. Retroviral recombination can occur when two genetically divergent genomic RNA molecules are present in the same viral particle, and arises during reverse transcription. Here we focuse on the mechanisms that have been proposed to account for the occurrence of recombination in retroviruses, from the strand displacement model, according to which recombination occurs during second DNA strand synthesis; to the description of the factors responsible for copy-choice recombination during first DNA strand synthesis, such as the presence of breaks, pause sites, or secondary structures in the genomic RNA. Most of these models have been supported by experimental data obtained from in vitro reconstituted systems or from cell infection studies using academic model sequences. The situation in vivo must be more complex, since several factors come into play when recombination involves relatively distant isolates, as in the case of inter-subtype recombination. At present, it is clear that further studies are needed in order to evaluate whether a prevailing mechanism exists for in vivo recombination, and will also be essential for understanding how the underlying mechanisms of recombination contribute to the evolution of HIV.
Reactions of indolic nitrones and N-heteroaromatic bases under irradiation and chemical oxidation (2003)

The spin adducts formed under UVA irradiation or in the presence of mild oxidants in the reaction of a number of heteroaromatic bases and two indolic nitrones have been characterized by means of ESR spectroscopy. The formation of the spin adducts is explained via the Forrester–Hepburn mechanism, while the occurrence of inverted spin trapping is excluded. Photolysis of nitrones 1 and 2 or of the corresponding cyclic hydroxamic acids resulted in the formation of the related acyl nitroxides.
Long-term Outcomes of Immediate Loading of Short Implants: A Controlled Retrospective Cohort Study (2016)

The immediate loading of short implants is not a risk factor for treatment success. This could be related to the good bone quality and the achievement of adequate primary stability.
Gamma-Aminobutyric Acid Production Using Immobilized Glutamate Decarboxylase Followed by Downstream Processing with Cation Exchange Chromatography (2013)

We have developed a gamma-aminobutyric acid (GABA) production technique using his-tag mediated immobilization of Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamate to GABA. The GAD was obtained at 1.43 g/L from GAD-overexpressed E. coli fermentation and consisted of 59.7% monomer, 29.2% dimer and 2.3% tetramer with a 97.6% soluble form of the total GAD. The harvested GAD was immobilized to metal affinity gel with an immobilization yield of 92%. Based on an investigation of specific enzyme activity and reaction characteristics, glutamic acid (GA) was chosen over monosodium glutamate (MSG) as a substrate for immobilized GAD, resulting in conversion of 2.17 M GABA in a 1 L reactor within 100 min. The immobilized enzymes retained 58.1% of their initial activities after ten consecutive uses. By using cation exchange chromatography followed by enzymatic conversion, GABA was separated from the residual substrate and leached GAD. As a consequence, the glutamic acid was mostly removed with no detectable GAD, while 91.2% of GABA was yielded in the purification step.
Neurophysiological mechanisms of temporal discrimination in the goldfish (1980)

Following the observation that phase-locking error in auditory neurons accounts for pure tone frequency discrimination behavior in the goldfish (R. Fay, Nature 275, 320–322), it was hypothesized that temporal discrimination using a broad class of quasiperiodic signals could be similarly explained. Temporal discrimination was studied psychophysically by classically conditioning goldfish to suppress respiration when presented with a slow sinusoidal change in the modulation rate (fm) of sinusoidally amplitude modulated (SAM) signals. The jnd for modulation period (ΔP ms) was measured as a function of fm (20–200 Hz) and of modulation depth, m, (100%-6.25%), for 800-Hz tone and noise carriers at 35 dB SL. Modulation period histograms were obtained for 60 auditory (saccular) neurons in response to the same set of SAM signals used in the psychophysical studies. For all modulated signals, the psychophysical ΔPs were linearly related to period histogram variance, indicating that measures of temporal neural error can predict temporal discriminability for a wide range of quasiperiodic signals. However, these results did not conform to the prediction from pure tone studies that ΔP is a linear function of the standard deviation of neural error. [Work supported by NIH grants 5 R01 NS 15268 and 1 K04 NS 00479.]
Supplementary Figure 1 from <i>NOTCH</i> Signaling Is Required for Formation and Self-Renewal of Tumor-Initiating Cells and for Repression of Secretory Cell Differentiation in Colon Cancer (2023)

Supplementary Figure 1 from &lt;i&gt;NOTCH&lt;/i&gt; Signaling Is Required for Formation and Self-Renewal of Tumor-Initiating Cells and for Repression of Secretory Cell Differentiation in Colon Cancer
ChemInform Abstract: C‐Glucopyranosyl Derivatives from Readily Available 2,3,4,6‐Tetra‐O‐ benzyl‐α‐D‐glucopyranosyl Chloride. (1987)

Abstract Silver(I)‐induced coupling of the α‐D‐glucopyranosyl chloride (I) with the silyl enol ethers (II) or m‐dimethoxybenzene (V) produces the α‐ and β‐C‐glucopyranosyl derivatives (IV) and (VI).