gebhart/patrepeval huggingface dataset:
Treatment with bone maturation and average lifespan of HPP model mice by AAV8-mediated neonatal gene therapy via single muscle injection (2021)

Hypophosphatasia (HPP) is an inherited skeletal disease characterized by defective bone and tooth mineralization due to a deficiency in tissue-nonspecific alkaline phosphatase (TNALP). Patients with the severe infantile form of HPP may appear normal at birth, but their prognosis is very poor. To develop a practical gene therapy for HPP, we endeavored to phenotypically correct TNALP knockout (<i>Akp2</i> <sup><i>-/-</i></sup> ) mice through adeno-associated virus type 8 (AAV8) vector-mediated, muscle-directed, TNALP expression. Following treatment of neonatal <i>Akp2</i> <sup><i>-/-</i></sup> mice with a single intramuscular injection of ARU-2801 (AAV8-TNALP-D10-vector) at 1.0 × 10<sup>12</sup> vector genomes/body, high plasma ALP levels (19.38 ± 5.02 U/mL) were detected for up to 18 months, and computed tomography analysis showed mature bone mineralization. Histochemical staining for ALP activity in the knee joint revealed ALP activity on the surface of the endosteal bone of mice. Throughout their lives, the surviving treated <i>Akp2</i> <sup><i>-/-</i></sup> mice exhibited normal physical activity and a healthy appearance, whereas untreated controls died within 3 weeks. No ectopic calcification or abnormal calcium metabolism was detected in the treated mice. These findings suggest that ARU-2801-mediated neonatal intramuscular gene therapy is both safe and effective, and that this strategy could be a practical option for treatment of the severe infantile form of HPP.
Difference Raman spectroscopy of DNA molecules (2015)

In this paper the micro-Raman spectra of calf DNA for different points of DNA sample have been recorded. The Raman spectra were made with help of difference Raman spectroscopy technique. Raman spectra were recorded with high spatial resolution from different points of the wet and dry samples in different spectral range (100÷4000cm−1) using two lasers: argon (514.5 nm) and helium -neon (632.8 nm). The significant differences in the Raman spectra for dry and wet DNA and for different points of DNA molecules were observed. The obtained data on difference Raman scattering spectra of DNA molecules may be used for identification of DNA types and for analysis of genetic information associated with the molecular structure of this molecule.
Detachable balloon embolization of an latrogenic aortocoronary arteriovenous fistula combined with aortocoronary bypass PTCA: A case report (1992)

Coronary artery bypass surgery was performed in a 54-year-old male, and one of the grafts was inadvertently sutured to the vena cordis magna instead of to the left anterior descending coronary artery (LAD). Four years later the patient observed a progression of symptoms including episodes of angina pectoris at rest. Coronary angiography showed stenosis of one of the bypass grafts and notable dilatation of an iatrogenic arteriovenous (A-V) fistula. The stenosed bypass graft was dilated and the A-V fistula occluded by use of a detachable balloon. Embolization was performed rather than surgery, as the LAD was found to be a poor surgical target. The patient's symptoms improved after the procedure; he was followed for 18 months during which time his condition remained stable.
Learning 3-D object orientation from images (2009)

We propose a learning algorithm for estimating the 3-D orientation of objects. Orientation learning is a difficult problem because the space of orientations is non-Euclidean, and in some cases (such as quaternions) the representation is ambiguous, in that multiple representations exist for the same physical orientation. Learning is further complicated by the fact that most man-made objects exhibit symmetry, so that there are multiple ldquocorrectrdquo orientations. In this paper, we propose a new representation for orientations-and a class of learning and inference algorithms using this representation-that allows us to learn orientations for symmetric or asymmetric objects as a function of a single image. We extensively evaluate our algorithm for learning orientations of objects from six categories.
Periacetabular osteotomy in the treatment of neurogenic acetabular dysplasia (1999)

We carried out the Bernese periacetabular osteotomy for the treatment of 13 dysplastic hips in 11 skeletally mature patients with an underlying neurological diagnosis. Seven hips had flaccid paralysis and six were spastic. The mean age at the time of surgery was 23 years and the mean length of follow-up was 6.4 years. Preoperatively, 11 hips had pain and two had progressive subluxation. Before operation the mean Tönnis angle was 33 degrees, the mean centre-edge angle was -10 degrees, and the mean extrusion index was 53%. Postoperatively, they were 8 degrees, 25 degrees and 15%, respectively. Pain was eliminated in 7 patients and reduced in four in those who had preoperative pain. One patient developed pain secondary to anterior impingement from excessive retroversion of the acetabulum. Four required a varus proximal femoral osteotomy at the time of the pelvic procedure and one a late varus proximal femoral osteotomy for progressive subluxation. Before operation no patient had arthritis. At the most recent follow-up one had early arthritis of the hip (Tönnis grade I) and one had advanced arthritis (Tönnis grade III). Our results suggest that the Bernese periacetabular osteotomy can be used successfully to treat neurogenic acetabular dysplasia in skeletally mature patients.
Synthesis and Anti-HIV-Evaluation of Novel Andrographolide Derivatives (2012)

A series of Andrographolide derivatives were described and evaluated for their in vitro anti-HIV activity. Compound 11 and 21 showed significant anti-HIV-1 activity in C8166 cells. Since compound 11 had no cytotoxicity, it was expected to be new lead for further development of anti-AIDS agents. Keywords: Andrographolide, Derivatives, Anti-HIV, C8166 cells, Natural products, Synthesis, Acquired immune deficiency syndrome, human immunodeficiency virus, RDCSY- I capillary apparatus, lymphocyte counts
Supplementary Data from Targeting Multiple EGFR-expressing Tumors with a Highly Potent Tumor-selective Antibody–Drug Conjugate (2023)

&lt;p&gt;In vivo Efficacy of ABBV-321 tumor targeting.&lt;/p&gt;
Kakkonda Geothermal Power Plant (1979)

A brief general description is given of a geothermal resource. Geothermal exploration in the Takinoue area is reviewed. Geothermal drilling procedures are described. The history of the development at the Takinoue area (the Kakkonda Geothermal Power Plant), and the geothermal fluid characteristics are discussed. The technical specifications of the Kakkonda facility are shown. Photographs and drawings of the facility are included. (MHR)
[Sonography for monitoring kidney transplants]. (1982)

Ultrasonography is very useful for judging the position and course of renal transplants. With this method extra- and intrarenal complications can be detected very accurately. The sonographic examination is quite harmless and puts little strain on the patient. It can be carried out a few days after the operation. This initial sonogram, obtained as early as possible, makes it easier to recognize and to interpret later changes more promptly and correctly. The results of sonographic examinations in 18 patients with renal transplants are presented. They show that a normal sonogram reliably excludes extrarenal complications such as fluid collection or obstruction as well as rejection of the transplant. All cases of rejection correlated to clearly abnormal sonographic findings.
Synthetic tracheal mucus with native rheological and surface tension properties (2013)

In this study, the development of a model tracheal mucus with chemical composition and physical properties (bulk viscoelasticity and surface tension) matched to that of native tracheal mucus is described. The mucus mimetics (MMs) were formulated using components that are abundant in tracheal mucus (glycoproteins, proteins, lipids, ions, and water) at concentrations similar to those found natively. Pure solutions were unable to achieve the gel behavior observed with native mucus. The addition of a bifunctional cross-linking agent enabled control over the viscoelastic properties of the MMs by tailoring the concentration of the cross-linking agent and the duration of cross-linking. Three MM formulations with different bulk viscoelastic properties, all within the normal range for nondiseased tracheal mucus, were chosen for investigation of surfactant spreading at the air-mimetic interface. Surfactant spread quickly and completely on the least viscoelastic mimetic surface, enabling the surface tension of the mimetic to be lowered to match native tracheal mucus. However, surfactant spreading on the more viscoelastic mimetics was hindered, suggesting that the bulk properties of the mimetics dictate the range of surface properties that can be achieved.
Reducing the chattering using the generalized variable structure control applied to a manipulator arm (2002)

The authors present experimental results, in step response, of the generalized (GVS) and the classical (CVS) variable structure control law for control of a manipulator. The methodology of synthesis has been given for the implementation of this new control law GVS. Experimental results have been presented for the three axes of robot. A comparison of performances of the CVS and GVS algorithms is been undertaken for parameter variations of the plant and for external disturbances. Obtained results show that the two controls are robust to parameter variations of the plant (payload variation) and insensitive to external disturbances, since the dynamics of system is fixed by the sliding surface. In sliding mode, the system evolves as a system of first order. The rapidity of the two control laws is comparable (the time response is practically the same). The chattering due to the discontinuity of control has been reduced by the GVS control in comparison to the CVS control. Finally, obtained results from implementation show advantages of this new technique of GVS control of viewpoint of the chattering, as well as the robustness of the two algorithms of variable structure control to errors of modelization and to external disturbances; this despite the linear approximation made on the dynamic model of robot. Therefore, the variable structure controllers present a solution to problems of the control of nonlinear systems and especially in the domain of robotics.
Efficient removal of caesium ions from aqueous solution using a calix crown ether in ionic liquids: mechanism and radiation effect (2010)

The removal of radioactive (137)Cs from nuclear waste is of great importance for both the environment and energy saving. Herein, we report a study on the removal of Cs(+) using a calix crown ether bis(2-propyloxy)calix[4]crown-6 (BPC6) in ionic liquids [C(n)mim][NTf(2)], where [C(n)mim](+) is 1-alkyl-3-methylimidazolium and [NTf(2)](-) is bis(trifluoromethylsulfonyl)imide. The BPC6/[C(n)mim][NTf(2)] system is highly efficient in removing Cs(+) from aqueous solution, even at a low concentration of BPC6. HNO(3) and metal ions such as Na(+), Al(3+) in the aqueous phase interfered with the extraction of Cs(+) by competitive interaction with BPC6 and/or salting-out effect. UV analysis confirmed that the extraction of Cs(+) by the BPC6/[C(n)mim][NTf(2)] system involves a dual extraction mechanism, i.e., via exchange of BPC6.Cs(+) complex or Cs(+) by [C(n)mim](+). Irradiation of [C(4)mim][NTf(2)] dramatically decreases Cs(+) partitioning in the ionic liquid phase by the competitive interaction of radiation-generated H(+) with BPC6, while irradiation of BPC6/[C(4)mim][NTf(2)] decreases Cs(+) partitioning more markedly due to the radiolysis of BPC6.
The Mechanism of the Suppressive Action of Bromocriptine on Adrenocorticotropin Secretion in Patients with Cushing′s Disease and Nelson′s Syndrome* (1980)

The oral administration of 2.5 mg bromocriptine elicited a significant suppression of plasma ACTH and cortisol in 6 of 13 patients with untreated Cushing′s disease. In the responding patients, mean plasma ACTH levels were higher and pituitary tumor size was larger than in the nonresponders. In two patients with Nelson′s syndrome, plasma ACTH was diminished by 70% and 30%, respectively, from 4-6 h after 2.5 mg bromocriptine. In one patient with ectopic ACTH secretion from a bronchial carcinoid, plasma ACTH was suppressed by 32% from 3-5 h after 2.5 mg bromocriptine. The effects of bromocriptine and cyproheptadine on the secretion of ACTH from trypsin-dispersed tumor cells prepared from the ACTH-secreting rat pituitary tumor 7315a were investigated in vitro. Bromocriptine (1 µM) inhibited ACTH secretion by 40% (P < 0.01), while 1 JUM cyproheptadine inhibited ACTH secretion by 25% (P < 0.01). On the basis of 1) the direct effects of bromocriptine and cyproheptadine on ACTH secretion by rat pituitary tumor cells, 2) the suppressive effect of bromocriptine in a patient with ectopic ACTH secretion, and 3) the increasing sensitivity of ACTH secretion to bromocriptine with increasing pituitary tumor size, we suggest that one mechanism of action of bromocriptine is a direct effect on the ACTH-secreting pituitary microadenoma n patients with Cushing′s disease and Nelson′s syndrome.
Vinylic polymerization of bicyclo[2.2.1]hept-2-ene by Co(II)-catalysis (1998)

The vinylic polymerization of bicyclo[2.2.1]hept-2-ene (norbornene) with Co(II) compounds, such as Co(II) stearate, substituted bis(1,3-diketo)cobalt(II), Co(dppe)Cl2, and the metallocene [η5-(C5Me5)Co-η2-Cl]2, in chlorobenzene activated with methylaluminoxane (MAO) is reported. MAO* synthesized by the hydrolysis of trimethylaluminium in chlorobenzene instead of toluene increases the catalytical activity strongly, and a turn over of 2.7 tons of poly(2,3-bicyclo[2.2.1]hept-2-ene) per mol cobalt per hour was achieved. The polymers obtained are amorphous (WAXS). They show weight-average molecular weights up to M̄w = 1.5 · 106 and are soluble in chlorobenzene, 1,2-dichlorobenzene, cyclohexane, and decahydronaphthalene.
Microwave holography: Applications and techniques (1977)

The techniques and applications of microwave holography are described. The connections with related fields, acoustical holography, and quasi-holography are summarized.
“Hands free for intervention”, a new approach for transoral endoscopic surgery (2015)

Abstract A standard method for transoral diagnostic and dissection of tumour is the endoscopic examination plus microscopic and laser supported surgery. For endoscopic examination a set of rigid endoscopes with different angles of view are on the market and in use. To simplify the diagnostic examination, a first idea was to use an endoscope with flexible angles of view. A further step of this approach is the electrical angulation of the endoscopic view to enable an adaption on the surgery site without using the hands. Thus the hands are free for intervention. This paper shows a first implementation of a prototype of an electrical operated endoscope for a free hand imaging in transoral interventions.
Rapid non-destructive detection of sub-surface Cu in silicon-on-insulator wafers by optical second harmonic generation (2015)

Time dependent second harmonic optical signals were measured across silicon-on-insulator (SOI) wafer coupons contaminated by Cu-63 ion implanted into the buried oxide (BOX) and near the SOI/BOX and BOX/Bulk interfaces. Average signals after 1 second of exposure for all spatial points were compared between wafers and used to differentiate contamination levels post ion-implantation.
Genotyping of clinical isolates of fluconazole-resistant Candida albicans (2013)

The aim of the study was to compare the discriminatory power of RAPD-PCR method with using RSD10 primer and microsatellite analysis with using (GACA)4 primer for genotyping clinical isolates of fluconazole-resistant C. albicans. Isolates were received from patients of Children's Memorial Health Institute in Warsaw. However in the case of both tested methods low number of amplified fragments was generated, nine and six different genotypes were distinguished from microsatellite analysis and RAPD-PCR respectively. An evident increase in the number of generated amplicons was achieved when PCR amplification was performed with both primers simultaneously. Surprisingly, such increase did not resulted in better discriminatory power. Only six different genotypes were distinguished, and 31 out of 44 (70%) strains tested were classified as one genotype.
Endoplasmic reticulum–resident protein 57 (ERp57) oxidatively inactivates human transglutaminase 2 (2018)

Transglutaminase 2 (TG2) is a ubiquitously expressed, intracellular as well as extracellular protein with multiple modes of post-translational regulation, including an allosteric disulfide bond between Cys-370-Cys-371 that renders the enzyme inactive in the extracellular matrix. Although recent studies have established that extracellular TG2 is switched "on" by the redox cofactor protein thioredoxin-1 (TRX), it is unclear how TG2 is switched "off." Here, we demonstrate that TG2 oxidation by small-molecule biological oxidants, including glutathione, cystine, and hydrogen peroxide, is unlikely to be the inactivation mechanism. Instead, endoplasmic reticulum (ER)-resident protein 57 (ERp57), a protein in the ER that promotes folding of nascent proteins and is also present in the extracellular environment, has the cellular and biochemical characteristics for inactivating TG2. We found that ERp57 colocalizes with extracellular TG2 in cultured human umbilical vein endothelial cells (HUVECs). ERp57 oxidized TG2 with a rate constant that was 400-2000-fold higher than those of the aforementioned small molecule oxidants. Moreover, its specificity for TG2 was also markedly higher than those of other secreted redox proteins, including protein disulfide isomerase (PDI), ERp72, TRX, and quiescin sulfhydryl oxidase 1 (QSOX1). Lastly, siRNA-mediated ERp57 knockdown in HUVECs increased TG2-catalyzed transamidation in the extracellular environment. We conclude that, to the best of our knowledge, the disulfide bond switch in human TG2 represents the first example of a post-translational redox regulatory mechanism that is reversibly and allosterically modulated by two distinct proteins (ERp57 and TRX).
In Vitro Degradation of a Poly(Propylene Fumarate)/ β -Tricalcium Phosphate Composite Orthopaedic Scaffold (1997)

This study involves investigating the in vitro degradation of a poly(propylene fumarate) (PPF) based composite material for orthopaedic applications. The effects of PPF molecular weight, PPF to vinyl monomer ratio, and solid phase content were studied. Mechanical properties, pore morphology, and sample mass loss were analyzed over a 12-week period of degradation. An initial increase in both compressive modulus and strength was seen for all formulations incorporating high molecular weight PPF. The PPF/monomer ratio was not seen to have a significant effect on any observations. Incorporation of β-tricalcium phosphate (β-TCP) resulted in an increase in mechanical properties and had no effect on weight loss. A composite formulation with an initial PPF/β-TCP ratio of 1.0 g/0.66 g exhibited an initial compressive strength of 2.60 MPa, which rose to 9.38 MPa at 3 weeks, and fell to 3.24 MPa at 7 weeks into the study. The initial modulus of 62.0 MPa for the same formulation increased to 250 MPa at 3 weeks, and fell to 63.7 MPa at 7 weeks. These studies further show that PPF/β-TCP composite scaffolds can be fabricated exhibiting initial mechanical properties similar to human trabecular bone and maintain these properties over several weeks of degradation.
Early versus delayed autologous transplantation after immunomodulatory agents‐based induction therapy in patients with newly diagnosed multiple myeloma (2011)

The current results indicated that, in transplantation-eligible patients who receive IMiDs as initial therapy followed by early stem cell mobilization, delayed SCT results in similar overall survival compared with early SCT. It is noteworthy that an excellent 4-year survival rate of >80% was observed among transplantation-eligible patients who received initial therapy with LD regardless of the timing of transplantation.
Drones-aided Asset Maintenance in Hospitals (2022)

The rapid outbreak of COVID-19 pandemic invoked scientists and researchers to prepare the world for future disasters. During the pandemic, global authorities on healthcare urged the importance of disinfection of objects and surfaces. To implement efficient and safe disinfection services during the pandemic, robots have been utilized for indoor assets. In this paper, we envision the use of drones for disinfection of outdoor assets in hospitals and other facilities. Such heterogeneous assets may have different service demands (e.g., service time, quantity of the disinfectant material etc.), whereas drones have typically limited capacity (i.e., travel time, disinfectant carrying capacity). To serve all the facility assets in an efficient manner, the drone to assets allocation and drone travel routes must be optimized. In this paper, we formulate the capacitated vehicle routing problem (CVRP) to find optimal route for each drone such that the total service time is minimized, while simultaneously the drones meet the demands of each asset allocated to it. The problem is solved using mixed integer programming (MIP). As CVRP is an NP-hard problem, we propose a lightweight heuristic to achieve sub-optimal performance while reducing the time complexity in solving the problem involving a large number of assets.
3D video: Depth coding based on inter-component prediction of block partitions (2012)

This paper presents a new approach for 3D video coding, where the video and the depth component of an MVD representation are jointly coded in an integrated framework. This enables a new type of prediction for exploiting the correlations of video and depth signals in addition to existing methods for temporal and inter-view prediction. Our new method is referred to as inter-component prediction and we adopt it for predicting non-rectangular partitions in depth blocks. By dividing the block into two regions, each represented with a constant value, such block partitions are well-adapted to the characteristics of depth maps. The results show that this approach reduces the bit rate of the depth component by up to 11% and leads to an increased quality of rendered views.
An ISFET based analogue ratiometric method for DNA methylation detection (2014)

This paper presents the concept of a ratiometric approach for DNA methylation detection using the “Methylation Cell” for the indication of epigenetic abnormalities related to cancer. The “Methylation Cell” allows real-time detection of pH signals resulting from DNA based reactions using ISFET sensors, performing continuous computation of the ratio of DNA methylation, giving a discrete output signal when DNA methylation exceeds a certain predefined percentage. Fabricated in a typical 0.35μm CMOS process, it uses current-mode translinear circuits to perform computation in low power. Experimental results are presented, demonstrating its capabilities through an integration in a Lab-on-Chip (LoC) set-up using a microfluidic assembly.