MP19-12 INJECTION OF MESENCHYMAL STEMS CELLS IN RATS WITH CHRONIC CYSTITIS IMPROVES DETRUSOR CONTRACTILITY (2015)

You have accessJournal of UrologyStem Cell Research1 Apr 2015MP19-12 INJECTION OF MESENCHYMAL STEMS CELLS IN RATS WITH CHRONIC CYSTITIS IMPROVES DETRUSOR CONTRACTILITY Jessica Hammett, Cody Thorson, Felicia Lane, Gamal Ghoniem, Os Steward, and Huiyi Chang Jessica HammettJessica Hammett More articles by this author , Cody ThorsonCody Thorson More articles by this author , Felicia LaneFelicia Lane More articles by this author , Gamal GhoniemGamal Ghoniem More articles by this author , Os StewardOs Steward More articles by this author , and Huiyi ChangHuiyi Chang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1004AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Chronic cystitis or inflammation of the bladder can lead to structural changes of the detrusor resulting in a small, contracted, low compliance, hemorrhagic, or irritative bladder with detrusor over- or under-activity. Unfortunately, there are few treatment options for improving bladder function and severely contracted bladders may have to be removed or augmented in order to preserve upper urinary tract function. In this experiment we aim to investigate the effect of rat derived mesenchymal stem cells on a chronic cyclophosphamide (CYP) induced cystitis in a rodent model. METHODS A chronic cystitis was induced through intraperitoneal injection of CYP (75 mg/kg) every other day for a total of 8 days in 40 Sprague Dawley rats. 20 animals (Groups 2 and 4) received the detrusor injections of mesenchymal rat stem cells (1 × 10^6) labeled with Green Fluorescent Protein (GFP). At 48 hours Group 1 (CYP controls) and Group 2 (stem cell Rx) were terminated. Urodynamic evaluation was conducted prior to termination on day 10 in Groups 3 (CYP control) and 4 (stem cell Rx). Immunohistochemistry and morphology of the bladder was performed. RESULTS At 48 hours there no significant difference in bladder wall thickness (p=0.167) or CGRP expression (p=0.645) between groups 1 (CYP controls) and 2 (stem cell Rx). H&E staining and GFP expression at 48 hours and 10 days showed the stem cells survived and did not spread from the injection sites. Groups 3 (CYP controls) and 4 (stem cell Rx) underwent urodynamic evaluation on day 10. Group 4 had significantly improved bladder compliance (p=0.047) and contraction amplitude (p=0.041) compared to Group 3. In addition, there was a significant decrease (p<0.0001) of non-voiding contractions between Groups 3 and 4. No significant difference was seen when analyzing maximum intravesical pressure, contraction duration, resting pressure, intracontraction interval, and bladder capacity (Table1). The bladder wall thickness was not significantly different between Groups 3 and 4 (p=0.605). Compared to Group 3, the expression of CGRP integrated density (p=0.036) at 10 days showed a significantly increase in Group 4. CONCLUSIONS In conclusion, we have provided evidence that mesenchymal stem cell therapy in the setting of a severe inflammatory cystitis improves bladder compliance and detrusor contractility. Statistical analysis of urodynamic measurements in Group 3 (CYP controls) and 4 (stem cell Rx) at 10 days CYP controls Stem cell Rx P value N=8 N=9 Max IVP (cm H2O) 48 ± 4 65 ± 9 0.114 RP (cm H20) 8 ± 4 9 ± 2 0.903 Abs Amp (cm H20) 40 ± 2 56 ± 6 0.041* CD (s) 31 ± 7 27 ± 2 0.912 ICI (s) 107 ± 16 108 ± 13 0.984 NVC 1 ± 1 7 ± 1 < 0.0001* Bladder capacity (mL) 0.33 ± 0.11 0.28 ± 0.05 0.695 Bladder compliance 0.65 ± 0.23 0.21 ± 0.08 0.047* Max IVP - maximum intravesical pressure. RP - resting pressure. Abs Amp - absolute amplitude. CD - contraction duration. ICI - intercontraction intervals. NVC - non-voiding contractions © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193 Issue 4S April 2015 Page: e219-e220 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.Metrics Author Information Jessica Hammett More articles by this author Cody Thorson More articles by this author Felicia Lane More articles by this author Gamal Ghoniem More articles by this author Os Steward More articles by this author Huiyi Chang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...