gebhart/patrepeval huggingface dataset:
Assays for Toxicity Studies in <i>C. elegans</i> With Bt Crystal Proteins (2006)

Caenorhabditis elegans is well suited for toxicological studies owing to its established biology, short generation time, large brood size, and readily scorable life traits. Quantitative parameters of C. elegans that can be assayed include growth, size, progeny production, behavior, and mortality. Qualitative parameters of toxicity, such as changes in appearance or movement, can also be determined. This chapter describes four assays we have used for analyzing the toxic effects of Bacillus thuringiensis crystal proteins toward C. elegans. The assays are quantitative growth measurement, brood size measurement, and determination of lethal concentration, as well as a qualitative health assay based on worm appearance. Although these assays are described for crystal proteins, the approaches are suited for the studies of other toxins with C. elegans.
Efficient Resource Optimization in Wireless Networks: A Deep-Learning Assisted Approach (2018)

peer reviewed
Deux bibliothèques (2016)

Une entente conclue en 2008 entre Québec et la Ville de Montréal comportait un programme de rénovation, d’agrandissement et de construction de bibliothèques dans les arrondissements de Montréal. Des concours d’architecture ont été lancés par le Bureau du design de Montréal ; cinq projets sont terminés ou en chantier, dont une nouvelle bibliothèque à Pierrefonds qui doit ouvrir ses portes d’ici 2017.La bibliothèque du BoiséL’utilisatio
Piezoelectric ceramic mechanical and electrical stress study (1994)

A study of published literature and information from piezoelectric ceramic vendors and underwater sound transducer designers has been undertaken to establish mechanical and electrical operating limits for transducers. It appears that operation up to 3.9–5.9 kV/cm (10–15 V/mil) rms and 69–103 MPa (10–15 kpsi) peak compression is achievable in practical sonar transducers.
Two-photon fluorescence cross-correlation spectroscopy as a potential tool for high-throughput screening of DNA repair activity (2005)

Several lines of evidence indicate that differences in DNA repair capacity are an important source of variability in cancer risk. However, traditional assays for measurement of DNA repair activity in human samples are laborious and time-consuming. DNA glycosylases are the first step in base excision repair of a variety of modified DNA bases. Here, we describe the development of a new sensitive DNA glycosylase assay based on fluorescence cross-correlation spectroscopy (FCCS) with two-photon excitation. FCCS was applied to the measurement of uracil DNA glycosylase activity of human cell extracts and validated by comparison with standard gel electrophoresis assay. Our results indicate that FCCS can be adapted to efficient assays for DNA glycosylase activity in protein extracts from human cells. This method has a potential for the development of automated screening of large number of samples.
Chlorpropamide-Induced Immune Hemolytic Anemia (1970)

Acute intravascular hemolysis developed while a patient was taking the oral sulfonylurea, chlorpropamide. An IgG antibody was present in the patient's serum that in the presence of chlorpropamide and fresh serum, brought about the lysis of complement-sensitive red cells from patients with paroxysmal nocturnal hemoglobinuria. The antibody was found to cross-react with other sulfonylureas but not with sulfisoxazole. Red cells from some patients with paroxysmal nocturnal hemoglobinuria were not lysed by this "innocent-bystander" reaction.
Updates in sepsis management: new guidelines for sepsis care (2012)

Actualización en
Reversal in fatigued athletes of a defect in interferon γ secretion after administration ofLactobacillus acidophilus (2006)

This is the first evidence of a T cell defect in fatigued athletes, and of its reversal following probiotic therapy.
Adsorption of diclofenac onto different biochar microparticles: Dataset – Characterization and dosage of biochar (2017)

Due to its wide occurrence in water resources and toxicity, pharmaceuticals and personal care products are becoming an emerging concern throughout the world. Application of residual/waste materials for water remediation can be a good strategy in waste management as well as in waste valorization. Herein, this dataset provides information on biochar application for the removal of emerging contaminant, diclofenac from water matrices. The data presented here is an extension of the research article explaining the mechanisms of adsorption diclofenac on biochars (Lonappan et al., 2017 [1]). This data article provides general information on the surface features of pine wood and pig manure biochar with the help of SEM and FTIR data. This dataset also provides information on XRD profiles of pine wood and pig manure biochars. In addition, different amounts of biochars were used to study the removal of a fixed concentration of diclofenac and the data is provided with this data set.
Allergenic .alpha.-methylene-.gamma.-butyrolactones. Stereospecific synthesis of (+)- and (-)-.gamma.-methyl-.alpha.-methylene-.gamma.-butyrolactones. A study of the specificity of (+) and (-) enantiomers in inducing allergic contact dermatitis (1982)

The enantiomers of gamma-methyl-alpha-methylene-gamma-butyrolactone have been prepared stereospecifically from (R)- and (S)-glutamic acid. Three groups of guinea pigs have been sensitized (Feund complete adjuvant technique) to the (+) isomer, the (-) isomer, and the (+/-) mixture. The animals have been tested with each of the enantiomers and with a mixture of the compounds. Only the (-) enantiomer showed some specificity: guinea pigs sensitized to this enantiomer react weakly to the other compound; in turn, animals sensitized to the (+) enantiomer react similarly to both antipodes. Interestingly, reaction to the (+/-) mixture in each group of guinea pigs was the sum of skin responses to the individual enantiomer. These results should be contrasted with sensitization to (+)- and (-)-frullanolides, sesquiterpene lactones for which strong stereospecificity was observed.
On complexity theory, exergy, and industrial ecology (2003)

Industrial ecology has been defined by Graedel and Allenby (1995) as “… the means by which humanity can deliberately and rationally approach and maintain a desirable carrying capacity, given continued economic, cultural and technological evolution. The concept requires that an industrial system be viewed not in isolation from its surrounding systems, but in concert with them.” The Institute of Electrical and Electronic Engineers (IEEE) Electronics and the Environment Committee (1995) has defined industrial ecology as “… the objective, multidisciplinary study of industrial and economic systems, and their linkages with fundamental natural systems.” Tibbs (1992) describes industrial ecology as follows: “… industrial ecology involves designing industrial infrastructures as if they were a series of interlocking manmade ecosystems interfacing with the natural global ecosystem”. And O’Rourke et al. (1996) characterize industrial ecology as “… bringing systems thinking in ecology together with systems engineering (for design of products and processes) and economics”While much of the literature on industrial ecology suggests that it originated in the early 1990s, Erkman (1997) and O’Rourke et al. (1996) trace its roots back to the early 1970s. One thread of development, which has been largely overlooked, can be traced to the Systems, Man, and Cybernetics Society of the Institute of Electrical and Electronic Engineers. In the early 1970s, Koenig, and later his son and their students at Michigan State, in collaboration with Chandrashekar and his students at the University of Waterloo, Canada, developed analytical tools, based on systems theory. These tools are used to analyze and design engineering systems so that they are as efficient and effective as possible, while minimizing their environmental impact. These tools are more sophisticated than any this author has seen in the industrial ecology literature. (Koenig and Tummala 1972; Koenig et al. 1972, 1975; Wong 1979; Wong and Chandrashekar 1982; Chinneck 1983; Chinneck and Chandrashekar 1984; Koenig and Tummala 1991; Tummala and Koenig 1993; Saama et al. 1994; Koenig and Cantlon 1998, 1999).
Shotgun proteomics study of early biofilm formation process of Acidithiobacillus ferrooxidansATCC 23270 on pyrite (2013)

Acidithiobacillus ferrooxidans is a chemolithoautotrophic, mesophilic Gram-negative bacterium able to oxidize ferrous iron, sulfur, and metal sulfides. It forms monolayer biofilms where extracellular polymeric substances are essential for cell attachment and metal sulfide leaching. High-throughput proteomics has been applied to study the early process of biofilm formation on pyrite by At. ferrooxidans ATCC 23270. After 24 h contact with the mineral, planktonic and sessile (biofilm) cell subpopulations were separated and proteins extracted. In total, 1319 proteins were detected in both samples. Sixty-two of these were found to be increased in biofilms. Additionally, 25 proteins were found to be decreased in the biofilm cell subpopulation. Three transcriptional factors were found to be increased or decreased among both cell subpopulations, suggesting their potential involvement in the regulation of these processes. Although no significant differences were observed for the known proteins related to ferrous iron and sulfur oxidation pathways among both cell subpopulations, the results presented here show that the early steps of At. ferrooxidans biofilm formation consist of a set of metabolic adaptations following cell attachment to the mineral surface. Functions such as extracellular polymeric substances biosynthesis seem to be pivotal. This first high-throughput proteomic study may also contribute to the annotation of several unknown At. ferrooxidans proteins found.
Virtual Screening for Transition State Analogue Inhibitors of IRAP Based on Quantum Mechanically Derived Reaction Coordinates (2015)

Transition state and high energy intermediate mimetics have the potential to be very potent enzyme inhibitors. In this study, a model of peptide hydrolysis in the active site of insulin-regulated aminopeptidase (IRAP) was developed using density functional theory calculations and the cluster approach. The 3D structure models of the reaction coordinates were used for virtual screening to obtain new chemical starting points for IRAP inhibitors. This mechanism-based virtual screening process managed to identify several known peptidase inhibitors from a library of over 5 million compounds, and biological testing identified one compound not previously reported as an IRAP inhibitor. This novel methodology for virtual screening is a promising approach to identify new inhibitors mimicking key transition states or intermediates of an enzymatic reaction.
Development and Validation of a Liquid Chromatography Tandem Mass Spectrometry Method for Simultaneous Determination of Four Anthocyanins in Human Plasma after Black Currant Anthocyanins Ingestion (2009)

Black currant anthocyanins consist of delphinidin-3-glucoside (1), delphinidin-3-rutinoside (2), cyanidin-3-glucoside (3), and cyanidin-3-rutinoside (4). A liquid chromatography tandem mass spectrometry method for simultaneous determination of four anthocyanins in human plasma was developed and validated. Samples were prepared using solid phase extraction, followed by chromatographic separation with a reverse phase C(18) column with gradient elution using mobile phases containing water, acetonitrile, and formic acid. The quantification of four anthocyanins was determined by multiple reaction monitoring using electrospray ionization. The method showed good selectivity, sensitivity (limits of quantification for four anthocyanins were 0.2 nmol/L), linearity (0.2-20 nmol/L; r > 0.999), intra- and interday precision, accuracy (<14%), and recovery (62.5-85.7%). Analyte stability was investigated in detail. This method was successfully applied to the determination of delphinidin-3-glucoside (1), delphinidin-3-rutinoside (2), cyanidin-3-glucoside (3), and cyanidin-3-rutinoside (4) concentrations in human plasma after ingestion of a single dose of black currant anthocyanins (87.9 micromol (58.8 mg) total anthocyanins).
Reversal of the transformed phenotype and inhibition of peptidylglycine α‐monooxygenase in Ras‐transformed cells by 4‐phenyl‐3‐butenoic acid (2004)

Recent studies have shown that the proliferation of some tumor cells is dependent on autocrine growth loops that require amidated autocrine growth factors. Peptidylglycine alpha-monooxygenase (PAM) is required for amidation of these growth factors and, therefore, this enzyme is an attractive target for anti-tumor compounds. 4-Phenyl-3-butenoic acid (PBA) is an irreversible turnover-dependent inhibitor of PAM in vitro and has been shown to decrease lung cancer cell proliferation by inhibiting the synthesis of amidated growth factors. We show here that PBA (0.1 mg/mL) inhibits the growth of Ras-transformed epithelial cells (WB-Ras) but has little effect on the proliferation of normal epithelial cells (WB-Neo). The methyl ester derivative of PBA (PBA-Me) at 10-fold lower concentration also exhibits a selective inhibition of Ras-transformed cell growth compared to normal epithelial cell growth. In addition, PBA produces a significant upregulation of gap junctional communication between WB-Ras cells following 2-5 day treatments, with a corresponding increase in the degree of connexin 43 phosphorylation and an increase in the number of connexin 43-containing plasma membrane gap junction plaques. Western blot analyses indicate no effect of PBA on the proportion of p21 Ras in the membrane versus cytosolic fractions or on p44/42 MAP kinase phosphorylation. Furthermore, the cell morphology of PBA-treated WB-Ras cells is altered, so as to more closely resemble that of non-transformed WB-Neo cells. PAM activity was assayed in both WB-Ras and WB-Neo cells, and we demonstrate that PBA at long treatment times (4 days) inhibits PAM activity in both cell types at concentrations that produce selective growth inhibition of WB-Ras cells. Shorter PBA treatment times (24 h), however, inhibit PAM activity in WB-Ras but not WB-Neo cells, an effect that was mimicked by PBA-Me. Taken together, these results clearly demonstrate that PBA returns Ras-transformed cells to a more normal phenotype, a finding consistent with the known increased dominance of the Ras signaling pathway in transformed epithelial cells.
Realization of a Distributed Bragg Reflector for Propagating Guided Matter Waves (2011)

We report on the experimental study of a Bragg reflector for guided, propagating Bose-Einstein condensates. A one-dimensional attractive optical lattice of finite length created by red-detuned laser beams selectively reflects some velocity components of the incident matter wave packet. We find quantitative agreement between the experimental data and one-dimensional numerical simulations and show that the Gaussian envelope of the optical lattice has a major influence on the properties of the reflector. In particular, it gives rise to multiple reflections of the wave packet between two symmetric locations where Bragg reflection occurs. Our results are a further step towards integrated atom-optics setups for quasi-cw matter waves.
Real-time partitioned convolution for Ambiophonics surround sound (2002)

Ambiophonics, as one of the most realistic music reproduction methods, requires multi-channel convolution with very long impulse responses for creation of believable reverberation. It is shown how the heavy processing task required for a real-time Ambiophonics system, or similar convolution-intensive system, can be handled by a low-cost personal computer, by means of partitioned convolution. The only theoretical advantage of partitioned convolution is that it provides low input/output delay. However, since the intensive part of it is very easily made optimal for the target platform, which normally is not the case for the standard overlap-and-save algorithm, it often provides the fastest convolution as well.
Design issues of a multi-functional intelligent thermal test die (2002)

Thermal characterization of IC packages and packaging technologies is becoming a key task in thermal engineering. To support this by measurements, we developed a family of thermal test chips that allows a wide range of possible applications. Our chips are based on the same basic cell that is mainly covered by dissipating resistors and also contains a frequency output temperature sensor. These basic cells are organized into arrays of different size. The arrays are designed so that larger arrays can also be built for tiling up larger package cavities. The first member of the family, TMC81, has been manufactured and measurements show that the goals aimed for at the design stage have been achieved.
SiP module mold flowability experiment result and simulation study (2018)

SiP turns to be complex due to increasing the number of components in a given space, narrow gap between die-to-passive or passive-to-passive and narrow clearance between mold top to components. Therefore, molding process becomes a critical process to have better reliability due to complex topography to minimize incomplete filling, air trap due to unbalanced flow during the process and solder extrusion or bump bridge due to voiding between solder interconnection. For these challenges, iNEMI has initiated SiP Module Moldability project which is to understand the filling characteristics for several different SiP module designs with 4 types of mold resin systems. This paper presents the outcome from a comprehensive study on the performance of SiP module moldability with various components geometries (different components densities on substrate, component gap & clearance) and 4 different mold resins through simulation and experiments. This study resulted in that all 9 different SiP modules could molded without any significant failures such as incomplete filling issues for different type of 4 mold resins. This is verified by not only from the experiment but also simulation result that no strong correlation between SiP module designs over mold resin systems for moldability performance.
Development of an Automatic Generation System for BWR Re-Startup Plans. (1995)

system to automatically generate re-startup plans for BWR plants has been developed. By including consideration of high speed re-startup after reactor shutdown, where xenon By including consideration of high speed re-startup after reactor shutdown, where xenon By including consideration of high speed re-startup after reactor shutdown, where xenon current reactor conditions. For this purpose, not only BWR core managing expertise in a knowledge base, but also core simulation programs are used. To realize both higher accuracy and shorter computing time of core characteristics predictions by the simulation programs, the error tendency between the simulation results and actual plant data is learned using an artificial neural network. To make the plan for re-startup operation, first, allowable xenon concentrations and corresponding control rod patterns that satisfy thermal constraints at the rated power are estimated. Secondly, based on the estimation results, control rod withdrawal sequence, reactor power, control rod pattern and core flow rate for each time are determined. A prototype system was developed to evaluate its performance. The system was capable of generating a plan automatically within 30min.
Chapter 7. Stimuli-responsive Supramolecular Gels (2018)

Soft materials that respond to external stimuli such as light, heat, voltage, magnetic field, mechanical stress, ions, or pH are important in a wide range of applications. Supramolecular gels derived from low-molecular-weight organic molecules (LMWGs) have generated significant attention because of their interesting stimuli-responsive physicochemical properties. For instance, by virtue of π-conjugation, gelators containing aromatic units exhibit excellent optical and electronic properties, which can be further modulated or controlled using external stimuli. We discuss recent developments in stimuli-responsive supramolecular gels with the help of selected examples.
Uropathogenic Escherichia coli Flagella Aid in Efficient Urinary Tract Colonization (2005)

In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxing) from the intracellular environment. IBC dispersal is a key step that results in the spread of bacteria over the epithelial surface to initiate additional rounds of IBC formation. We investigated the role of flagella in mediating adherence and motility during UTI, hypothesizing that the dispersion of the IBC would be incomplete in the absence of motility, thus interrupting the IBC pathway and attenuating the infection. Using gfp reporter fusions, the expression of the flagellar class I flhDC and class III fliC genes was monitored to track key points of regulation throughout the pathogenic cascade. In vitro, growth under conditions promoting motility resulted in the robust expression of both fusions. In contrast, only the class I fusion produced significant expression throughout early stages of IBC development including the dispersion stage. Thus, unlike in vitro modeling of motility, the regulatory cascade appeared incomplete in vivo. Throughout IBC formation, nonmotile DeltafliC mutants achieved the same number of IBCs as the wild-type (wt) strain, demonstrating that flagella are neither essential nor required for first- or second-generation IBC formation. However, in competition experiments between wt and DeltafliC strains, the wt was shown to have a fitness advantage in persisting throughout the urinary tract for 2 weeks, demonstrating a subtle but measurable role for flagella in virulence.
Treatment of port-wine stains at 577 nm: clinical results. (1987)

A pulsed, dye laser at 577 nm was used to treat 65 patients with port-wine stains. Results clearly indicate that the laser parameters of dose, wavelength, and pulse duration contribute to the outcome of laser treatment of port-wine stains.
Self-Assembly of Stimuli-Responsive Water-Soluble [60]Fullerene End-Capped Ampholytic Block Copolymer (2005)

A well-defined, water-soluble, pH and temperature stimuli-responsive [60]fullerene (C(60)) containing ampholytic block copolymer of poly((methacrylic acid)-block-(2-(dimethylamino)ethyl methacrylate))-block-C(60) (P(MAA-b-DMAEMA)-b-C(60)) was synthesized by the atom transfer radical polymerization (ATRP) technique. The self-assembly behavior of the C(60) containing polyampholyte in aqueous solution was characterized by potentiometric and conductometric titration, dynamic light scattering (DLS), and transmission electron microscopy. This amphiphilic mono-C(60) end-capped block copolymer shows enhanced solubility in aqueous medium at room and elevated temperatures and at low and high pH but phase separates at intermediate pH between 5.4 and 8.8. The self-assembly of the copolymer is different from that of P(MAA-b-DMAEMA). Examination of the association behavior using DLS revealed the coexistence of unimers and aggregates at low pH at all temperatures studied, with the association being driven by the balance of hydrophobic and electrostatic interactions. Unimers and aggregates of different microstructures are also observed at high pH and at temperatures below the lower critical solution temperature (LCST) of PDMAEMA. At high pH and at temperatures above the LCST of PDMAEMA, the formation of micelles and aggregates coexisting in solution is driven by the combination of hydrophobic, electrostatic, and charge-transfer interactions.