gebhart/patrepeval huggingface dataset:
Tough and Alkaline-Resistant Mussel-Inspired Wet Adhesion with Surface Salt Displacement via Polydopamine/Amine Synergy (2019)

The mussel-inspired catechol-based strategy has been well recognized as a promising alternative to design and exploit new generation adhesive materials applicable in many fields, ranging from biomedical adhesives to coatings of biomedical devices and engineering applications. However, in situ achievement of tough adhesion capability to substrate surfaces (e.g., minerals) is severely limited under the physiological environment or seawater condition (namely, relatively high salinity and mild alkalinity). In this work, a facile and versatile approach is proposed to in situ achieve robust wet adhesion in aqueous solutions of high salinity and mild alkalinity, via integrating primary amines into mussel-inspired polydopamine (PDA). By using a surface forces apparatus (SFA), the corresponding interaction behaviors have been systematically investigated. The strong wet adhesion was demonstrated and achieved via a synergetic effect of amine and PDA to the wet surfaces, including the surface salt displacement assisted by primary amine, strong adhesion to substrates facilitated by the catechol groups on PDA moieties, and enhanced cohesion through their cation-π interactions. Our results provide useful insights into the design and development of high-performance underwater adhesives and water-resistance materials.
ESEM-EDX Microanalysis at Bone-Implant Region on Immediately Loaded Implants Retrieved Postmortem (2022)

The high percentage of BA3 and BA4 in mandibular blocks associated with low presence of BA2 suggested the fast formation of compact bone tissue after 7 months from the implant placement. The presence of BA2 in maxilla blocks suggested active bone remodeling still present after 7 months. Ti particles were observed throughout the ROIs with and without bone remodeling activity. ESEMEDX resulted as a suitable technique to obtain more complete information on microchemistry composition and density/mineralization of bone around implants. For clinical significance, maxillary and mandibular peri-implant bone revealed different mineralization patterns, which means different healing times. The presence of Ti particles at the bone-implant interface and ion translocation, likely due to wear forces in the times following the implant insertion, do not affect the bone remodeling process.
RESEARCH ON ALGAE, BLUE-GREEN ALGAE, AND PHOTOTROPHIC NITROGEN FIXATION AT THE INTERNATIONAL RICE RESEARCH INSTITUTE (1963-81), SUMMARIZATION, PROBLEMS , AND PROSPECTS1 (1982)

This paper provides g eneral i nformation on one of the major research areas of IRRI'S Soil Microbiology Department. Conclusions made after an extensive survey of the literature on the role of blue-green algae in rice cultivation are summarized. algae, and phototrophic nitrogen fixation is reviewed. The paper also provides information on problems encountered and prospects for future research. Microbiological research at IRRI on algae, blue-green
L’extrusion réactive une voie privilégiée pour la synthèse de nouveaux matériaux polymères et composites (2017)

International audience
Expression and characterization of a cytotoxic human-frog chimeric ribonuclease: potential for cancer therapy (1997)

Onconase is a cytotoxic ribonuclease with antitumor properties. A semisynthetic gene encoding the entire protein sequence was constructed by fusing oligonucleotides coding for the first 15 and last six of the 104 amino acid residues to a genomic clone that encoded the remaining amino acid residues. Additionally, the 15 N-terminal amino acid residues of onconase were replaced with the first 21 amino acid residues of the homologous human RNase, eosinophil-derived neurotoxin, EDN. Two versions of the hybrid EDN-onconase protein were cloned, expressed and purified. The chimera that contained a glycine in lieu of the aspartic acid present in native onconase (position 26 in the chimera) exhibited enzymatic activity more characteristic of EDN than native onconase and was considerably more active with respect to both RNase activity and cellular cytotoxicity than recombinant onconase. In contrast to native or recombinant onconase, the EDN chimera was recognized by anti-EDN polyclonal antibodies, demonstrating that the chimera also shared structural antigenic determinants to the human enzyme. These results demonstrate that a chimeric ribonuclease has cytotoxicity comparable to onconase in two out of four cell lines tested. The implications with regard to cancer therapy are presented.
Complex Involvement of Interleukin-26 in Bacterial Lung Infection (2021)

Pneumonia is a global cause of mortality, and this provides a strong incentive to improve the mechanistic understanding of innate immune responses in the lungs. Here, we characterized the involvement of the cytokine interleukin (IL)-26 in bacterial lung infection. We observed markedly increased concentrations of IL-26 in lower airway samples from patients with bacterial pneumonia and these correlated with blood neutrophil concentrations. Moreover, pathogen-associated molecular patterns (PAMPs) from both Gram-negative and -positive bacteria increased extracellular IL-26 concentrations in conditioned media from human models of alveolar epithelial cells, macrophages, and neutrophils <i>in vitro</i>. Stimulation with IL-26 inhibited the inherent release of neutrophil elastase and myeloperoxidase in unexposed neutrophils. This stimulation also inhibited the expression of activity makers in neutrophils exposed to <i>Klebsiella pneumoniae.</i> In addition, priming of human lung tissue <i>ex vivo</i> with exogenous IL-26 potentiated the endotoxin-induced increase in mRNA for other cytokines involved in the innate immune response, including the master Th17-regulator IL-23 and the archetype inhibitory cytokine IL-10. Finally, neutralization of endogenous IL-26 clearly increased the growth of <i>Klebsiella pneumoniae</i> in the macrophage culture. These findings suggest that IL-26 is involved in bacterial lung infection in a complex manner, by modulating critical aspects of innate immune responses locally and systemically in a seemingly purposeful manner and by contributing to the killing of bacteria in a way that resembles an antimicrobial peptide. Thus, IL-26 displays both diagnostic and therapeutic potential in pneumonia and deserves to be further evaluated in these respects.
Agricultural activities and risk of treatment for depressive disorders among the entire French agricultural workforce: the TRACTOR project, a nationwide retrospective cohort study (2023)

MIAI@Grenoble Alpes, and Mutualité Sociale Agricole.
Can Accelerated Reactions in Droplets Guide Chemistry at Scale? (2016)

Mass spectrometry (MS) is used to monitor chemical reactions in droplets. In almost all cases, such reactions are accelerated relative to the corresponding reactions in bulk, even after correction for concentration effects, and they serve to predict the likely success of scaled‐up reactions performed in microfluidic systems. The particular chemical targets used in these test studies are diazepam, atropine and diphenhydramine. In addition to a yes/no prediction of whether scaled‐up reaction is possible, in some cases valuable information was obtained that helped in optimization of reaction conditions, minimization of by‐products, and choice of catalyst. In a variant on the spray‐based charged droplet experiment, the Leidenfrost effect was used to generate larger, uncharged droplets and the same reactions were studied in this medium. These reactions were also accelerated but to smaller extents than in microdroplets, and they gave results that correspond even more closely to microfluidics data. The fact that MS was also used for online reaction monitoring in the microfluidic systems further enhances the potential role of MS in exploratory organic synthesis.
Electrical Properties of RF Sputtering Systems (1979)

A theory is developed that gives a relatively complete electrical characterization of rf sputtering systems. Three types of systems are analyzed: tuned substrate, driven substrate, and controlled area ratio of electrode (CARE) systems. The theory is applicable to any of these systems that do not use magnetic fields to confine the plasma. Given the input rf power and voltage at the target, and any other parameters that can be specified as independent variables (e.g., pressure, substrate drive voltage, tuning impedance, and system geometry), the theory provides explicit values for all dc and rf electrical parameters of the system. The dc bias developed at the substrate is explained and related to the resputtering energy. In addition, an approximate calculation is presented for the ion density in the plasma; this calculation allows a semiquantitative estimate of the rf voltage developed at the target for a given value of rf input power. It also shows the influence of pressure and frequency on rf sputtering system operation. Comparisons are made with real rf sputtering systems; these show that the theory is quite successful in predicting the operation of these systems. In addition, a much better understanding is achieved of some of the complex electrical phenomena encountered in these systems. The theory should prove useful both for new system design and for diagnostic work on existing equipment.
Dual busy tone multiple access (DBTMA)-performance results (2003)

The dual busy tone multiple access (DBTMA) scheme was designed for distributed multi-hop networks. The protocol uses the RTS/CTS dialogue to reserve the shared channel. In multi-hop networks, some nodes in the range of the transmitter and/or the range of the receiver might not be able to hear a successful RTS/CTS message exchange. This may lead to access collisions and data destruction. Hence we use two narrow bandwidth busy tones, to notify neighbor nodes of the on-going use of the channel. In this paper, we analyze the capacity of the DBTMA protocol. The effects of various parameters on the network utilization in multi-hop networks are also discussed. We compare, through analytical results and simulation means, the performance of the DBTMA protocol with schemes that solely use the RTS/CTS dialogue to prevent collisions and we show that DBTMA provides superior performance to such schemes.
Chitosan Containing Supported Ionic Liquid Phase Materials for CO2 Absorption (2016)

Here we present novel CO2 sorbents based on chitosan ionogels. The powder sorbents called inverse supported ionic liquid phase (SILP) materials were prepared by dissolving chitosan in various ionic liquids (ILs) followed by encapsulation of the ionogel droplets with nanoporous fumed silica. CO2 absorption was determined at 40 °C in the range of 200 to 5500 mbar. At 1 bar, absorption capacities of these materials were 0.1–0.8 mol kg–1; at 5 bar, values of 0.2–1.5 mol kg–1 were reached. A comparison of inverse SILP materials with and without chitosan dissolved in the applied IL indicated that the presence of chitosan increased the CO2 absorption efficiency of the materials. The aim of the study was also to compare the CO2 absorption in pure chitosan and chitosan dissolved in ILs. It was found that dissolution increases the absorption capacity of chitosan about 10 times.
Deletion of carboxypeptidase E in beta cells disrupts proinsulin processing and alters beta cell identity in mice (2022)

Abstract Carboxypeptidase E (CPE) facilitates the conversion of prohormones into mature hormones and is highly expressed in multiple neuroendocrine tissues. Carriers of CPE mutations have elevated plasma proinsulin and develop severe obesity and hyperglycemia. We aimed to determine whether loss of Cpe in pancreatic beta cells disrupts proinsulin processing and accelerates development of diabetes and obesity in mice. Pancreatic beta cell-specific Cpe knockout mice (β Cpe KO; Cpe fl/fl x Ins1 Cre/+ ) lack mature insulin granules and have elevated proinsulin in plasma; however, glucose-and KCl-stimulated insulin secretion in β Cpe KO islets remained intact. High fat diet-fed β Cpe KO mice showed comparable weight gain and glucose tolerance compared to Wt littermates. Notably, beta-cell area was increased in chow-fed β Cpe KO mice and beta-cell replication was elevated in β Cpe KO islets. Transcriptomic analysis of β Cpe KO beta cells revealed elevated glycolysis and Hif1α -target gene expression. Upon high glucose challenge, beta cells from β Cpe KO mice showed reduced mitochondrial membrane potential, increased reactive oxygen species, reduced MafA , and elevated Aldh1a3 transcript levels. Following multiple low-dose streptozotocin treatment, β Cpe KO mice had accelerated hyperglycemia with reduced beta-cell insulin and Glut2 expression. These findings suggest that Cpe and proper proinsulin processing are critical in maintaining beta cell function during the development of diabetes.
A phase III randomized study comparing perioperative nivolumab vs. observation in patients with localized renal cell carcinoma undergoing nephrectomy (PROSPER RCC). (2018)

TPS710 Background: The anti-PD-1 antibody nivolumab (nivo) improves overall survival (OS) in metastatic treatment refractory RCC and is generally tolerable. In 2017, there is no standard adjuvant therapy proven to increase OS over surgery alone in non-metastatic (M0) disease. Mouse solid tumor models have revealed an OS benefit with a short course of neoadjuvant PD-1 blockade compared to adjuvant therapy. Two ongoing phase 2 studies of perioperative nivo in RCC patients (pts) are showing preliminary feasibility and safety with no surgical delays/complications. PROSPER RCC will examine if the addition of perioperative nivo to radical or partial nephrectomy can improve clinical outcomes in pts with locally advanced RCC. We are implementing a three-pronged, multidisciplinary approach of presurgical priming with nivo followed by resection and adjuvant PD-1 blockade with the goal of increasing cure and recurrence-free survival (RFS) rates in M0 RCC. Methods: Tumor biopsy prior to randomization is mandatory to ensure RCC diagnosis but will also permit unparalleled correlative science in this global, unblinded, phase 3 National Clinical Trials Network randomized study. 766 pts with clinical stage ≥T2 or any node positive M0 RCC of any histology will be enrolled. The study arm will receive nivo 240mg IV for 2 doses prior to surgery followed by adjuvant dosing for 9 mo (q2 wks x 3 mo followed by q4 wks x 6 mo). The control arm will undergo the current standard of care: surgical resection followed by observation. Pts are stratified by clinical T stage, node positivity, and histology. There is 84.2% power to detect a 14.4% absolute increase in the primary endpoint of RFS from the ASSURE historical control of 55.8% to 70.2% at 5 yrs (HR 0.70). The study is also powered to detect a significant OS benefit (HR 0.67). Safety, feasibility, and quality of life are key secondary endpoints. PROSPER RCC exemplifies team science and incorporates a host of correlative work to examine the significance of the baseline immune milieu and changes induced by neoadjuvant priming and to identify predictive gene expression patterns. New collaborations welcomed. Clinical trial information: NCT03055013.
D2 receptors in Huntington's disease: positron emission tomography findings and clinical correlates (1990)

The relationship of dopamine receptor binding in the caudate nucleus and the putamen to neurological and neuropsychological functioning was examined in 21 patients with Huntington's disease (HD) and eight individuals at risk of developing Huntington's disease. A significant reduction in relative binding of [11C]3-N-methylspiperone to the dopamine receptor was found in both the caudate and putamen of HD patients. Binding in the caudate was correlated only with tests of rapid coding and set alternation, while binding in the putamen was correlated only with duration of illness. The findings indicate that the well-described atrophic changes in the striatum of Huntington's disease patients are accompanied by receptor alterations. They also support previous animal and human studies indicating that the caudate nucleus plays a larger role in cognition than in motor functions.
Enhancing Next-Generation Sequencing-Guided Cancer Care Through Cognitive Computing (2017)

The results of this study demonstrate that the interpretation and actionability of somatic next-generation sequencing results are evolving too rapidly to rely solely on human curation. Molecular tumor boards empowered by cognitive computing can significantly improve patient care by providing a fast, cost-effective, and comprehensive approach for data analysis in the delivery of precision medicine. Patients and physicians who are considering enrollment in clinical trials may benefit from the support of such tools applied to genomic data.
The connection between lymphangiogenic signalling and prostaglandin biology: A missing link in the metastatic pathway (2012)

Substantial evidence supports important independent roles for lymphangiogenic growth factor signaling and prostaglandins in the metastatic spread of cancer. The significance of the lymphangiogenic growth factors, vascular endothelial growth factor (VEGF)-C and VEGF-D, is well established in animal models of metastasis, and a strong correlation exits between an increase in expression of VEGF-C and VEGF-D, and metastatic spread in various solid human cancers. Similarly, key enzymes that control the production of prostaglandins, cyclooxygenases (COX-1 and COX-2, prototypic targets of Non-steroidal anti-inflammatory drugs (NSAIDs)), are frequently over-expressed or de-regulated in the progression of cancer. Recent data have suggested an intersection of lymphangiogenic growth factor signaling and the prostaglandin pathways in the control of metastatic spread via the lymphatic vasculature. Furthermore, this correlates with current clinical data showing that some NSAIDs enhance the survival of cancer patients through reducing metastasis. Here, we discuss the potential biochemical and cellular basis for such anti-cancer effects of NSAIDs through the prostaglandin and VEGF signaling pathways.
Pulse compression by coherent Raman scattering (2007)

The width of an ultraviolet pulse is compressed to sub-20 fs from 100 fs using a prism compressor by compensating the group delay dispersion among the Raman sidebands generated by coherent Raman scattering.
Bactericidal Activity and Toxicity of Iodine-Containing Solutions in Wounds (1982)

Complexing iodine with povidone (polyvinylpyrrolidone) or surfactants significantly limits the quantity of free iodine. Reduction of the free iodine level eliminates the adverse properties of staining, instability, and irritation and also alters bactericidal activity. Addition of detergents to create surgical scrub solutions further reduces the activity of iodine. In vitro testing indicated that the bactericidal activity of iodophors was inferior to that of uncomplexed aqueous iodine. In vivo tests proved that aqueous iodine significantly potentiated the development of infection. Although the povidone iodophor did not enhance the rate of wound or infection, it offered no therapeutic benefit when compared with control wounds treated with saline solution. Addition of detergents to the povidone iodophor was deleterious, with the wounds exposed to this combination displaying significantly higher infection rates than untreated control wounds. Based on these results, aqueous iodine solutions and iodophor surgical scrub solutions should not be used on broken skin. Aqueous iodophors can be used in wounds, but no therapeutic benefit from such use was found in this study.
Protein allergens of white-faced hornet, yellow hornet, and yellow jacket venoms (1978)

White-faced hornet, yellow hornet, and yellow jacket venoms have very similar protein compositions; each contains mainly three basic proteins. Two of these proteins have hyaluronidase and phospholipase activities and the third one, designated as antigen 5, is of as yet unidentified biochemical function. These three proteins have molecular weights of about 45 000, 35 000, and 25 000, respectively. The three proteins of white-faced hornet venom have been purified to near homogeneity, while this is the case only for antigen 5 of yellow hornet and yellow jacket venoms. Strong antigenic cross-reaction of the hyaluronidase from these three vespid venoms was observed using specific rabbit anti-venom sera, while weak cross-reactions of phospholipases and of antigen 5s were observed. All three proteins are active as allergens to varying degrees in vespid sensitive individuals. With each vespid venom its antigen 5 seems to be the major allergen. The results help to clarify the commonly observed varying degrees of multiple sensitivity of people to different vespids.
Thogoto virus ML protein is a potent inhibitor of the interferon regulatory factor-7 transcription factor (2009)

The tick-transmitted orthomyxovirus Thogoto virus (THOV) encodes the ML protein acting as a viral suppressor of the host interferon (IFN) system. Here, we describe that type I IFN is strongly induced in primary mouse embryo fibroblasts as well as plasmacytoid dendritic cells upon infection with a THOV mutant lacking the ML gene. However, wild-type THOV encoding ML suppresses induction of IFN by preventing the activation of members of the IFN regulatory factor (IRF) family. We found that reporter gene expression dependent on IRF3 and IRF7 was strongly inhibited by ML. Further experiments revealed that ML interacts with IRF7 and prevents dimerization of the transcription factor and its association with the coactivator TRAF6. Interestingly, another IRF7 activation step, nuclear translocation, is not affected by ML. Our data elucidate ML protein as a virulence factor with an IRF-specific IFN-antagonistic spectrum.
Remote Central‐to‐Axial Chirality Conversion: Direct Atroposelective Ester to Biaryl Transformation (2018)

Abstract A strategy for the remote central‐to‐axial chirality conversion by simultaneous planarization of an encoding and a transient stereocenter is presented. Based on a diastereoselective double addition of a chiral 1,5‐bifunctional organomagnesium alkoxide reagent to a broad range of aryl ester substrates, axially chiral biaryls are directly obtained upon in situ reduction. Various structurally distinct atropisomeric biaryl silanes that serve as valuable chiral biaryl anion surrogates are accessible in one step with e.r. values of up to 98:2.
Trace-level reuse (2003)

Trace-level reuse is based on the observation that some traces (dynamic sequences of instructions) are frequently repeated during the execution of a program, and in many cases, the instructions that make up such traces have the same source operand values. The execution of such traces will obviously produce the same outcome and thus, their execution can be skipped if the processor records the outcome of previous executions. This paper presents an analysis of the performance potential of trace-level reuse and discusses a preliminary realistic implementation. Like instruction-level reuse, trace-level reuse can improve performance by decreasing resource contention and the latency of some instructions. However, we show that trace-level reuse is more effective than instruction-level reuse because the former can avoid fetching the instructions of reused traces. This has two important benefits: it reduces the fetch bandwidth requirements, and it increases the effective instruction window size since these instructions do not occupy window entries. Moreover, trace-level reuse can compute all at once the result of a chain of dependent instructions, which may allow the processor to avoid the serialization caused by data dependences and thus, to potentially exceed the dataflow limit.