gebhart/patrepeval huggingface dataset:
Pathologic interaction between megakaryocytes and polymorphonuclear leukocytes in myelofibrosis (2000)

Idiopathic myelofibrosis (MF) is a myeloproliferative syndrome characterized by an increase in bone marrow collagen. Megakaryocytes (Mks), which store growth factors in their α granules, are known to be involved in the pathogenesis of MF. Previously, mice given bone marrow grafts infected with a retrovirus carrying murine thrombopoietin (TPO) complementary DNA developed a disease resembling human idiopathic MF. In this study, we used this murine model (TPO mice) to determine whether release of α granules is responsible for fibroblast activation and development of fibrosis. The intracellular trafficking of several α-granule proteins (von Willebrand factor, fibrinogen, and transforming growth factor β (TGFβ), which are stored in the granule matrix; and αIIbβ3 integrin and P-selectin (CD62p), which are located in the α-granule membrane) was studied with immune electron microscopy in bone marrow Mks from TPO mice. P-selectin immunolabeling increased consistently and was occasionally found lining the demarcation membrane system. Evidence of extensive emperipolesis was also found in TPO mouse Mks, involving almost exclusively neutrophil and eosinophil polymorphonuclear (PMN) cells with altered morphologic features. In parallel, the host Mks had myeloperoxidase-positive granules scattered in their cytoplasm, associated with marked ultrastructural cytoplasmic alterations and ruptured α-granule membranes. Similar observations were made in bone marrow biopsy specimens from 12 patients with idiopathic MF; indeed, there was an increased rate of emperipolesis involving mostly PMN cells, abnormal P-selectin expression, and mutual subcellular PMN and Mk alterations. This study indicates that in idiopathic MF, abnormal P-selectin distribution in Mks induces selective sequestration of PMN cells. This results in a release of α-granular proteins and growth factors, which in turn induces fibroblast activation and fibrosis deposition.
Successful Embolization of Persistent Endoleak From a Patent Inferior Mesenteric Artery (1997)

This case, which highlights the importance of documenting a patent IMA prior to AAA endografting, illustrates one option for the management of persistent collateral perfusion of endovascularly excluded aneurysms.
Effect of shearing‐induced lipolysis on foaming properties of milk (2023)

Shearing-induced lipolysis greatly affected the physical properties of milk samples and subsequently their foaming properties and foam structure. At the same FFA level, lipolysis induced by microfluidisation was much less detrimental to the foaming properties of milk than lipolysis induced by ultra-turraxing and homogenisation. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
One-pot preparation of methyl 2-diazo-3-oxopropionates comprising an aqueous ‘sulfonyl-azide-free’ (SAFE) diazo transfer step (2020)

Methyl 2-diazo-3-oxopropionates were obtained by in situ methoxycarbonylation of methyl ketones followed by diazo transfer onto active methylene group of the intermediate β-oxo esters. At the second stage, ‘sulfonyl-azide-free’ (SAFE) diazo transfer protocol in aqueous medium was employed.
A supply chain risk assessment model based on multistage influence diagram (2009)

Supply chain system is a dynamic network system with fuzzy edge, and how to assess the risks of this system has become the bottleneck of enterprises management. In the light of the influence diagram theory, this paper builds a risk assessment model of supply chain from the topology layer and function layer. The model graphically describes the elements of risk assessment process and takes into account both the uncontrollable and the controllable factors in the supply chain risk. This paper can provide help for the supply chain risk management in strategic and tactical decision-making.
Deep-ultraviolet LEDs fabricated in AlInGaN using MEMOCVD (2004)

In this paper we will present our recent work aimed at developing deep ultraviolet light-emitting diodes with emission from 250-280 nm. These devices were fabricated using AlGaN multiple quantum wells that were deposited on basal plane sapphire substrates using low-pressure MOCVD. Innovative MEMOCVD grown AlN buffer layers and AlN/AlGaN superalttices were also employed in the device structures to manage strain and allow the deposition of thick AlGaN layers, which was necessary to reduce the lateral spread resistance. Devices with square, multifinger and micro-LED geometries were fabricated and flip-chip mounted on AlN carriers for improved thermal management and light extraction. We have now succeeded in obtaining devices at 275 and 280 nm with cw powers in excess of 1.5 mW and pulsed powers well over 20 mW. Recently we have also succeeded in obtaining nearly milliwatt powers using an innovative micro-LED design at 250 nm. Now, for the first time, we also present dc operation of micro-pixel design deep UV LED.
µJ-level multi-cycle terahertz generation in a periodically poled Rb:KTP crystal (2021)

We demonstrate multi-cycle terahertz (MC-THz) generation in a 15.5 mm long periodically poled rubidium (Rb)-doped potassium titanyl phosphate (Rb:PPKTP) crystal with a poling period of 300 µm. By cryogenically cooling the crystal to 77 K, up to 0.72 µJ terahertz energy is obtained at a frequency of 0.5 THz with a 3 GHz bandwidth. A maximum internal optical-to-terahertz conversion efficiency of 0.16% is achieved, which is comparable with results achieved using periodically poled lithium niobate crystal. Neither photorefractive effects nor damage was observed with up to 900<i>m</i><i>J</i>/<i>c</i><i>m</i><sup>2</sup>, showing the great potential of Rb:PPKTP for multi-millijoule-level MC-THz generation.
Spectra of turbulent static pressure fluctuations in jet mixing layers (1977)

Spectral similarity laws are derived for the power spectra of turbulent static pressure fluctuations by application of dimensional analysis in the limit of large turbulent Reynolds number. The theory predicts that pressure spectra are generated by three distinct types of interaction in the velocity fields: a fourth order interaction between fluctuating velocities, an interaction between the first order mean shear and the third order velocity fluctuations, and an interaction between the second order mean shear rate and the second order fluctuating velocity. Measurements of one-dimensional power spectra of the turbulent static pressure fluctuations in the driven mixing layer of a subsonic, circular jet are presented, and the spectra are examined for evidence of spectral similarity. Spectral similarity is found for the low wavenumber range when the large scale flow on the centerline of the mixing layer is self-preserving. The data are also consistent with the existence of universal inertial subranges for the spectra of each interaction mode.
BAGEL2: mining for bacteriocins in genomic data (2010)

Mining bacterial genomes for bacteriocins is a challenging task due to the substantial structure and sequence diversity, and generally small sizes, of these antimicrobial peptides. Major progress in the research of antimicrobial peptides and the ever-increasing quantities of genomic data, varying from (un)finished genomes to meta-genomic data, led us to develop the significantly improved genome mining software BAGEL2, as a follow-up of our previous BAGEL software. BAGEL2 identifies putative bacteriocins on the basis of conserved domains, physical properties and the presence of biosynthesis, transport and immunity genes in their genomic context. The software supports parameter-free, class-specific mining and has high-throughput capabilities. Besides building an expert validated bacteriocin database, we describe the development of novel Hidden Markov Models (HMMs) and the interpretation of combinations of HMMs via simple decision rules for prediction of bacteriocin (sub-)classes. Furthermore, the genetic context is automatically annotated based on (combinations of) PFAM domains and databases of known context genes. The scoring system was fine-tuned using expert knowledge on data derived from screening all bacterial genomes currently available at the NCBI. BAGEL2 is freely accessible at http://bagel2.molgenrug.nl.
Analyzing Structure–Photophysical Property Relationships for Isolated T8, T10, and T12 Stilbenevinylsilsesquioxanes (2013)

Silsesquioxanes (SQs) are of considerable interest for hybrid electronic and photonic materials. However, to date, their photophysical properties have not been studied extensively, thus their potential remains conjecture. Here we describe the first known efforts to map structure-photophysical properties as a function of cage symmetry and size by comparing identically functionalized systems. Our focus here is on the solution photophysical properties of the title stilbenevinyl-SQs, which were characterized using single photon absorption, two-photon absorption, fluorescence emission, and fluorescence lifetime kinetics. We offer here the first detailed photophysical study of the larger pure T10 and T12 silsesquioxanes and show photophysical properties that differ as a function of size, especially in their fluorescence behavior, indicating that cage size and/or symmetry can strongly affect photophysical properties. We also find that they offer excitation-dependent emission (evidence of rare "red-edge" effects). The T10 stilbenevinyl-SQ offers up to a 10-fold increase in two-photon absorption cross section per chromophore over a free chromophore, signifying increased electronic coupling. The SQ cage compounds show "rise times" of 700-1000 fs and low anisotropy (~0.1) in fluorescence lifetime kinetic studies. These results indicate excited state energy transfer, unobserved for the free chromophores and unexpected for systems with "inert" silica cores and for 3-D hybrid molecular species. These findings provide the first detailed photophysical study of chromophore-functionalized T10 and T12 silsesquioxanes and show that SQs may be considered a separate class of compounds/materials with anticipated novel properties of value in developing new components for electronic and photonic applications.
Receptor-Mediated Endocytosis and Lysosomal Processing of Insulin-Like Growth Factor I by Mitogenically Responsive Cells* (1988)

The processing of insulin-like growth factor I (IGF-I) by MG-63, an IGF-I-responsive human osteosarcoma cell line, has been investigated. At 37 C, the binding of [125I] IGF-I to monolayers of MG-63 cells reaches a maximum after about 1 h and slowly declines thereafter. The addition of the lysosomotropic agents methylamine, chloroquine, and monensin to the binding medium prevents the decline in [125I]IGF-I binding observed in the untreated cells and causes a 1.5- to 3-fold increase in cell-associated radioactivity after 4 h. Leupeptin, an inhibitor of lysosomal proteases, and colchicine, an inhibitor of endosomal transport, also increase cell-associated [125I]IGF-I. Three observations indicate that the increased radioactivity associated with the treated cells is the result of intracellular accumulation of the ligands and not the result of an increase in cell surface IGF binding. First, no increase in [125I]IGF-I binding is observed in cells preincubated with methylamine at 37 C but transferred to 4 C (where endocytosis is inhibited) before the addition of the radiolabeled ligands. Second, the increased radioactivity bound by methylamine-treated cells is not removed by washing the cells with dilute acid, a treatment that removes surface-bound [125I]IGF-I. Third, in leupeptin-treated cells [125I]IGF-I accumulates in a subcellular fraction with properties characteristic of lysosomes. Both alpha IR-3 (100 nM), an antibody that specifically inhibits binding to the type I IGF receptor, and high concentrations of insulin (900 nM) inhibit the accumulation of [125I]IGF-I by methylamine-treated cells, indicating that internalization of IGF-I occurs through the type I IGF receptor and not through the type II IGF receptor or the IGF-binding protein(s) that is also present on these cells. These results demonstrate that in MG-63 cells IGF-I is endocytosed via the type I IGF receptor and that the endocytosed hormone is degraded, at least in part, in lysosomes. These findings are similar to those described for the processing of insulin and other growth factors by their target cells and extend further the homology between IGF-I and these other agents.
Reviewing Large LAMA2 Deletions and Duplications in Congenital Muscular Dystrophy Patients (2014)

Our findings show that this type of mutation is fairly frequent (18.4% of mutated alleles) and is underestimated in the literature. It is important to include the screening of large deletions/duplications as part of the genetic diagnosis strategy.
A case of Enteropathy Associated T-cell Lymphoma of small intestine diagnosed by double-balloon-endoscopy –A case report and brief review of literature- (2018)

A-54-year-old male presented epigastric pain, loss of appetite and body weight loss 3 months prior to admission. An abdominal CT showed the dilatation of small intestine. We diagnosed as Enteropathy Associated T-cell Lymphoma (EATL) / Monomorphic epitheliotropic intestinal TCL (MEITL) by biopsy specimens under double-balloon-endoscopy. He was evaluated as stage IVB according to Ann Arbor staging classification. He was treated with CHOP therapy for 2 cycles. EATL is one of the rare diseases in Japan and known of poor prognosis. Here we show the usefulness of double-balloon-endoscopy for the diagnosis of EATL with brief review of literature.
212 Driving Control of an Artificial Myocardium Driven by a Linear Actuator (2006)

Heart transplantation is reliable way to patients with severe cardiac failure. The limited number of available donor hearts promotes the establishment of alternative treatments for these patients. An artificial hearts has been used for such patient and has shown reliable results as a bridge to heart transplantation. However, direct contact between the material and the blood causes embolism from thrombus. Blood pressure is maintained by pushing the heart. The place which must be helped in the patient of severe congestive heart failure is contraction of the heart. The purpose of study is to develop an artificial myocardium that compress the dysfunctional heart from its surface.
A Study of Carry-Over and Histopathological Effects after Chronic Dietary Intake of Citrinin in Pigs, Broiler Chickens and Laying Hens (2020)

Citrinin (CIT) is a polyketide mycotoxin occurring in a variety of food and feedstuff, among which cereal grains are the most important contaminated source. Pigs and poultry are important livestock animals frequently exposed to mycotoxins, including CIT. Concerns are rising related to the toxic, and especially the potential nephrotoxic, properties of CIT. The purpose of this study was to clarify the histopathological effects on kidneys, liver, jejunum and duodenum of pigs, broiler chickens and laying hens receiving CIT contaminated feed. During 3 weeks, pigs (n = 16) were exposed to feed containing 1 mg CIT/kg feed or to control feed (n = 4), while 2 groups of broiler chickens and laying hens (n = 8 per group) received 0.1 mg CIT/kg feed (lower dose group) and 3 or 3.5 mg CIT/kg feed (higher dose group), respectively, or control feed (n = 4). CIT concentrations were quantified in plasma, kidneys, liver, muscle and eggs using a validated ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method. Kidneys, liver, duodenum and jejunum were evaluated histologically using light microscopy, while the kidneys were further examined using transmission electron microscopy (TEM). Histopathology did not reveal major abnormalities at the given contamination levels. However, a significant increase of swollen and degenerated mitochondria in renal cortical cells from all test groups were observed (p < 0.05). These observations could be related to oxidative stress, which is the major mechanism of CIT toxicity. Residues of CIT were detected in all collected tissues, except for muscle and egg white from layers in the lowest dose group, and egg white from layers in the highest dose group. CIT concentrations in plasma ranged between 0.1 (laying hens in lower dose group) and 20.8 ng/mL (pigs). In tissues, CIT concentrations ranged from 0.6 (muscle) to 20.3 µg/kg (liver) in pigs, while concentrations in chickens ranged from 0.1 (muscle) to 70.2 µg/kg (liver). Carry-over ratios from feed to edible tissues were between 0.1 and 2% in pigs, and between 0.1 and 6.9% in chickens, suggesting a low contribution of pig and poultry tissue-derived products towards the total dietary CIT intake for humans.
Deep Proteome Profiling of Circulating Granulocytes Reveals Bactericidal/Permeability-Increasing Protein as a Biomarker for Severe Atherosclerotic Coronary Stenosis (2012)

Coronary atherosclerosis represents the major cause of death in Western societies. As atherosclerosis typically progresses over years without giving rise to clinical symptoms, biomarkers are urgently needed to identify patients at risk. Over the past decade, evidence has accumulated suggesting cross-talk between the diseased vasculature and cells of the innate immune system. We therefore employed proteomics to search for biomarkers associated with severe atherosclerotic coronary lumen stenosis in circulating leukocytes. In a two-phase approach, we first performed in-depth quantitative profiling of the granulocyte proteome on a small pooled cohort of patients suffering from chronic (sub)total coronary occlusion and matched control patients using stable isotope peptide labeling, two-dimensional LC-MS/MS and data-dependent decision tree fragmentation. Over 3000 proteins were quantified, among which 57 candidate biomarker proteins remained after stringent filtering. The most promising biomarker candidates were subsequently verified in the individual samples of the discovery cohort using label-free, single-run LC-MS/MS analysis, as well as in an independent verification cohort of 25 patients with total coronary occlusion (CTO) and 19 matched controls. Our data reveal bactericidal/permeability-increasing protein (BPI) as a promising biomarker for severe atherosclerotic coronary stenosis, being down-regulated in circulating granulocytes of CTO patients.
CMOS Silicon Physical Unclonable Functions Based on Intrinsic Process Variability (2011)

This paper presents an extreme-low-power mixed-signal CMOS integrated circuit for product identification and anti-counterfeiting, which implements a physical unclonable function operating with a challenge-response scheme. We devise a series of circuits and algorithmic solutions based on the use of a process monitor and on the prediction of the erratic response bits which allow to suppress the effects of temperature, voltage supply and process variations in order to obtain a robust and reliable behavior.
Abstraction and representation of continuous variables in connectionist networks (1986)

A method is presented for using connectionist networks of simple computing elements to discover a particular type of constraint in multidimensional data. Suppose that some data source provides samples consisting of n-dimensional feature-vectors, but that this data all happens to lie on an m-dimensional surface embedded in the n-dimensional feature space. Then occurrences of data can be more concisely described by specifying an m-dimensional location on the embedded surface than by reciting all n components of the feature vector. The recoding of data in such a way is a form of abstraction. This paper describes a method for performing this type of abstraction in connectionist networks of simple computing elements. We present a scheme for representing the values of continuous (scalar) variables in subsets of units. The backpropagation weight updating method for training connectionist networks is extended by the use of auxiliary pressure in order to coax hidden units into the prescribed representation for scalar-valued variables.
Evolutionary Patterns in Chemical Composition and Biomechanics of Articulated Coralline Algae (2022)

Seaweeds inhabiting wave-battered coastlines are generally flexible, bending with the waves to adopt more streamlined shapes and reduce drag. Coralline algae, however, are firmly calcified, existing largely as crusts that avoid drag altogether or as upright branched forms with uncalcified joints (genicula) that confer flexibility to otherwise rigid thalli. Upright corallines have evolved from crustose ancestors independently multiple times, and the repeated evolution of genicula has contributed to the ecological success of articulated corallines worldwide. Structure and development of genicula are significantly different across evolutionary lineages, and yet biomechanical performance is broadly similar. Because chemical composition plays a central role in both calcification and biomechanics, we explored evolutionary trends in cell wall chemistry across crustose and articulated taxa. We compared the carbohydrate content of genicula across convergently-evolved articulated species, as well as the carbohydrate content of calcified tissues from articulated and crustose species, to search for phylogenetic trends in cell wall chemistry during the repeated evolution of articulated taxa. We also analysed the carbohydrate content of one crustose coralline species that evolved from articulated ancestors, allowing us to examine trends in chemistry during this evolutionary reversal and loss of genicula. We found several key differences in carbohydrate content between calcified and uncalcified coralline tissues, though the significance of these differences in relation to the calcification process requires more investigation. Comparisons across a range of articulated and crustose species indicated that carbohydrate chemistry of calcified tissues was generally similar, regardless of morphology or phylogeny; conversely, chemical composition of genicular tissues was different across articulated lineages, suggesting that significantly different biochemical trajectories have led to remarkably similar biomechanical innovations.
High Dielectric Films Prepared By The Nanotransfer Method (2007)

Fabrication technologies of high-density capacitor onto polymer substrate is shown using nanotransfer method. The formed PZT film capacitor has a dielectric constant of around 1000 that is over 10 times higher than that of previous one.