gebhart/patrepeval huggingface dataset:
Transfection of Sertoli cells ex vivo; Opening the door to the in vitro study of junction turnover in the seminiferous epithelium (2006)

Sertoli cells interact with adjacent Sertoli cells and with spermatids through elaborate intercellular adhesion junctions termed ectoplasmic specializations (ESs). Among adhesion molecules present at the sites is Nectin‐2. Tubulobulbar complexes have been proposed to play a role in junction internalization. Here we establish a culture model for exploring the role of tubulobulbar complexes during junction turnover. Cell clumps enriched for Sertoli cells were isolated from ten day‐old rat pups and cultured on Matrigel coated Transwells. These cells established a columnar morphology and formed identifiable basal ESs. Significantly, structures resembling tubulobulbar complexes developed at the junction sites. We generated eGFP‐tagged nectin‐2 constructs and transfected them first into Hela cells (cells that do not normally form adherens junctions) and then into our primary cultures of morphologically differentiated Sertoli cells. In Hela cells, eGFP‐Nectin‐2 localized to sites of intercellular contact between adjacent cells indicating that the protein was expressed and targeted to the plasma membrane where it appeared to establish adherens junctions. In Sertoli cells, the protein was localized to areas consistent with the position of ESs and to tubular structures associated with them. Primary Sertoli cell cultures may be a model for studying the role of tubulobulbar complexes in junction turnover. CIHR MOP 62728
Spectroscopic Properties of Tc(I) Tricarbonyl Species Relevant to the Hanford Tank Waste (2015)

Technetium-99 (Tc) exists predominately in soluble forms in the liquid supernatant and salt cake fractions of the nuclear tank waste stored at the U.S. DOE Hanford Site. In the strongly alkaline environments prevalent in the tank waste, its dominant chemical form is pertechnetate (TcO4-, oxidation state +7). However, attempts to remove Tc from the Hanford tank waste using ion-exchange processes specific to TcO4- only met with limited success, particularly processing tank waste samples containing elevated concentrations of organic complexants. This suggests that a significant fraction of the soluble Tc can be present as non-pertechnetate low-valent Tc (oxidation state < +7) (non-pertechnetate). The chemical identities of these non-pertechnetate species are poorly understood. Previous analysis of the SY-101 and SY-103 tank waste samples provided strong evidence that non-pertechnetate can be comprised of [Tc(CO)3]+ complexes containing Tc in oxidation state +1 (Lukens et al. 2004). During the last two years, our team has expanded this work and demonstrated that high-ionic-strength solutions typifying tank waste supernatants promote oxidative stability of the [Tc(CO)3]+ species (Rapko et al. 2013; Levitskaia et al. 2014). It also was observed that high-ionic-strength alkaline matrices stabilize Tc(VI) and potentially Tc(IV) oxidation states, particularly in presence organic chelators, suggesting that the relevant Tc compounds can serve as important redox intermediates facilitating the reduction of Tc(VII) to Tc(I). Designing strategies for effective Tc processing, including separation and immobilization, necessitates understanding the molecular structure of these non-pertechnetate species and their identification in the actual tank waste samples. To-date, only limited information exists regarding the nature and characterization of the Tc(I), Tc(IV), and Tc(VI) species. One objective of this project is to identify the form of non-pertechnetate in the Hanford waste. To do this, we are developing a spectral library of reference non-pertechnetate compounds that can be compared against actual waste samples. The emphasis of the fiscal year 2015 work was Tc(I) tricarbonyl [Tc(CO)3]+ compounds. The key findings are summarized below.
Synthesis of a protected 2-ethoxy-3-hydroxyethylfuran and its regioselectivity as a Diels–Alder diene on reaction with 3,5-dimethoxydehydrobenzene (2002)

As a potential route for the synthesis of naturally occurring benzisochromanequinones, 2-ethoxy-3-(1'-methoxymethoxyethyl)furan 15 was prepared and shown to react regioselectively with in situ generated 3,5-dimethoxydehydrobenzene to afford, after hydrolysis and alkylation, the target naphthalene 1-(6',8'-dimethoxy-1'-ethoxy-4'-isopropoxy-2'-naphthalenyl)-1-(methoxymethoxy)ethane 27.
<title>Digital image registration using structured genetic algorithm</title> (1992)

This paper describes a new genetic approach called the structured genetic algorithm (sGA) for automatic registration of digital images. The specialty of this genetic model lies primarily in its redundant genetic material and a gene activation mechanism which utilizes a multi-layered structure for the chromosome. The additional genetic material serves to retain multiple optional solution spaces in parameter optimization. The structured genetic model is applied here to minimize the registration measures in image transformations, as investigated by Fitzpatrick and Grefenstatte with the simple GA. The results demonstrate that sGA is a much faster and robust search method that is guaranteed to reach a global optimum by adaptively estimating the subspace from the maximum space during the evolutionary process. Preliminary experimental results are reported.
Mallarme's children: symbolism and the renewal of experience (2000)

In a narrative gracefully combining intellectual and cultural history, Richard Candida Smith unfolds the legacy of Stephane Mallarme (1842-1898), the poet who fathered the symbolist movement in poetry and art. The symbolists found themselves in the midst of the transition to a world in which new media devoured cultural products and delivered them to an ever-growing public. Their goal was to create and oversee a new elite culture, one that elevated poetry by removing it from a direct relationship to experience. Instead, symbolist poetry was dedicated to exploring discourse itself, and its practitioners to understanding how language shapes consciousness. Candida Smith investigates the intellectual context in which symbolists came to view artistic practice as a form of knowledge. He relates their work to psychology, especially the ideas of William James, and to language and the emergence of semantics. Through the lens of symbolism, he focuses on a variety of subjects: sexual liberation and the erotic, anarchism, utopianism, labor, and women's creative role. Paradoxically, the symbolists' reconfiguration of elite culture fit effectively into the modern commercial media. After Mallarme was rescued from obscurity, symbolism became a valuable commodity, exported by France to America and elsewhere in the market-driven turn-of-the-century world. Mallarme's Children traces not only how poets regarded their poetry and artists their art but also how the public learned to think in new ways about cultural work and to behave differently as a result.
Urinary symptom flare after brachytherapy for prostate cancer is associated with erectile dysfunction and more urinary symptoms before implantation (2006)

Radiation reaction and radiation sensitivity contribute to ED and greater LUTS in men who have had brachytherapy for prostate cancer. This contribution is evident, e.g. in men with ataxia-telangiectasia (ATM) gene mutations. Sequence variants in the ATM gene, particularly those that encode for an amino-acid substitution, are associated with adverse radiotherapy responses among patients treated with 125I prostate brachytherapy. Our finding of the association of urinary symptom flare with ED suggests it would be worthwhile to determine whether sildenafil is as effective in men with flare, and if not, whether higher sildenafil doses would be of value. Alternatively, alpha1-selective adrenoceptor-blocking agents, e.g. terazosin, combined with sildenafil, might be of benefit. Also, patients with a high IPSS before brachytherapy can be warned that they have a greater risk of flare and ED.
[Effect of total knee arthroplasty under computer navigation on intraoperative blood loss and joint function recovery]. (2020)

Under the guidance of computer navigation, total knee arthroplasty can prolong the operation time compared with single total knee arthroplasty, but it is more conducive to reduce perioperative blood loss, reduce the rate of postoperative allogeneic blood transfusion, ideal recovery of joint function, less complications, safety and reliability.
Near‐ and supercritical fluid solvents for living anionic polymerizations (1993)

Abstract Near‐ and supercritical fluids are investigated as solvents for anionic polymerizations of 1,3‐dienes. Solvent strength (solubility parameter) of mixtures of light hydrocarbons containing cyclohexane is shown to be adjustable using solvatochromic probe methods. The results indicate that these mixtures have solubility parameter values that are lower than conventional liquid solvents and represent a regime of solvent strength that has remained unexplored for ionic polymerizations. The anionic initiator 3‐methyl‐1,1‐diphenyl‐pentyllithium (MDPPL) was shown to be soluble in supercritical ethane and that its ultraviolet/visible absorption maximum was sensitive to systematic density changes of the supercritical fluid induced by pressure profiling. These results were extended to the anionic polymerization of isoprene in butane/cyclohexane mixtures.
Oxygen regulation of nitrate uptake in denitrifying Pseudomonas aeruginosa (1987)

Oxygen had an immediate and reversible inhibitory effect on nitrate respiration by denitrifying cultures of Pseudomonas aeruginosa. Inhibition of nitrate utilization by oxygen appeared to be at the level of nitrate uptake, since nitrate reduction to nitrite in cell extracts was not affected by oxygen. The degree of oxygen inhibition was dependent on the concentration of oxygen, and increasing nitrate concentrations could not overcome the inhibition. The inhibitory effect of oxygen was maximal at approximately 0.2% oxygen saturation. The inhibition appeared to be specific for nitrate uptake. Nitrite uptake was not affected by these low levels of aeration, and nitrite reduction was only partially inhibited in the presence of oxygen. The regulation of nitrate respiration at the level of transport by oxygen may represent a major mechanism by which the entire denitrification pathway is regulated in P. aeruginosa.
377. Genome-wide association analyses for various thermotolerance indicators measured during lactation in maternal-line pigs (2022)

Heat stress is a major welfare and economic issue in the swine industry. Genome-wide association studies (GWAS) represent a great tool to investigate the association of candidate genes and quantitative trait loci (QTL) with phenotypes of interest. Therefore, this study aimed to perform GWAS for six indicators of thermotolerance (automatically-measured vaginal temperature; ear, shoulder, rump, and tail temperatures; and respiration rate) in lactating sows to identify genomic regions and candidate genes influencing these traits in commercial Landrace × Large White sows during lactation stage under heat stress conditions. Sixteen genomic regions were identified on chromosomes 1, 2, 3, 5, 6, 7, 8, 10, 12, 13, 14, and 16, with ten of these genomic regions related with known QTL associated with health and production traits. These findings highlight the importance of thermoregulation indicators on the performance and well-being of pigs, and consequently adding such traits to the current selection indexes.
Pricing user-sanctioned dynamic fast-lanes driven by content providers (2015)

The notion of “fast-lanes” for prioritising certain Internet content on residential broadband access links is being vigorously debated today. While Internet Service Providers (ISPs) see fast-lane revenue as an imperative for their economic sustainability, end-users and several Content Providers (CPs) feel threatened by the violation of network neutrality. In this paper we argue that fast-lanes can present a win-win-win situation for ISPs, CPs, and end-users alike, if implemented in a way that gives each party appropriate control knobs. To this end, we present an architecture in which fast-lanes are dynamically created and destroyed on-the-fly on a per-session basis using input from all three parties - this departs from current proposals that have largely focused on static fast-lanes. We then present a simplified economic model that demonstrates the benefits for each entity: users can get enhanced Quality of Experience (QoE) at no extra cost, CPs can monetise user-satisfaction using business-models of their discretion, and the ISP can experiment with two-sided pricing models of their choice. We evaluate our proposal using real traffic traces and multiple pricing models, and set the ground for a deeper study into the economics of dynamic fast-lanes.
Análisis exclusivo de la letra de Zapatero: grafología de un acomplejado (2009)

El escritor y periodista Cesar Vidal es ademas un experto grafologo, pese a que rara vez ha dado a conocer sus numerosos estudios en esta materia. En rigurosa exclusiva para Epoca, Vidal analiza en estas paginas la caligrafia de Jose Luis Rodriguez Zapatero, un estudio extraordinariamente revelador de la personalidad del presidente del Gobierno
The Mechanism of the Baeyer-Villiger Reaction of Benzaldehydes with Peroxybenzoic Acid (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Mechanism of the Baeyer-Villiger Reaction of Benzaldehydes with Peroxybenzoic AcidYOSHIRO OGATA, IWAO TABUSHI, and HIROYUKI AKIMOTOCite this: J. Org. Chem. 1961, 26, 12, 4803–4807Publication Date (Print):December 1, 1961Publication History Published online1 May 2002Published inissue 1 December 1961https://pubs.acs.org/doi/10.1021/jo01070a006https://doi.org/10.1021/jo01070a006research-articleACS PublicationsRequest reuse permissionsArticle Views342Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Structural and genetic characterization of fish skeletal muscle tropomyosins (2002)

Tropomyosins were purified from the fast skeletal muscle of several fish species. While their behaviors on two-dimensional PAGE (2D-PAGE) were examined, their thermodynamic properties were compared by differential scanning calorimetry (DSC). Furthermore, full- and partial lengths of cDNAs encoding fast skeletal muscle tropomyosins were cloned from white croaker and walleye pollack, respectively, and their nucleotide and deduced amino acid sequences were determined. It was suggested that the differences observed in the above two analytical parameters among fish species were due to only a few amino acid replacements in the molecule.
Feeding colostrum or a 1:1 colostrum:whole milk mixture for 3 days after birth increases serum immunoglobulin G and apparent immunoglobulin G persistency in Holstein bulls (2020)

Conflicting reports exist on whether prolonged IgG consumption can further increase serum IgG in neonatal calves. Given that higher serum IgG in neonates has lifelong benefits, our objective was to determine whether serum IgG can be increased by providing multiple meals containing IgG to neonatal calves. Twenty-seven Holstein bulls were all fed 1 colostrum meal (7.5% body weight; 62 g of IgG/L) at 2 h after birth and randomly assigned to be fed (5% body weight) colostrum (COL; n = 9), whole milk (WM; n = 9), or a 1:1 colostrum:whole milk mixture (MX; n = 9) every 12 h from 12 to 72 h. Serum IgG was measured at 1, 2, 3, 6, 9, 11, and 12 h after birth. After the 12-h meal, IgG was determined at 0.5-h intervals until 16 h and then at 1-h intervals from 16 to 24 h. Serum IgG was then measured at 27 h, then every 6 h from 30 to 60 h. From 60 to 64 h, IgG was measured every 0.5 h, then at 65 and 66 h, and then every 2 h until 72 h. Serum IgG increased rapidly between 2 and 12 h for all calves. A treatment × time interaction occurred as serum IgG began to diverge between treatments after they were fed at 12 h; the interaction was greatest over the entire period for COL compared with both MX and WM and was greater for MX than for WM. Maximum IgG concentrations (C<sub>max</sub>) were 30.4 ± 0.8, 27.2 ± 0.8, and 23.9 ± 0.8 g/L for COL, MX, and WM, respectively. Although MX C<sub>max</sub> was equivalent to both COL and WM C<sub>max</sub>, COL C<sub>max</sub> was greater than WM C<sub>max</sub>. Feeding COL and MX also prolonged the time to reach C<sub>max</sub>. Respectively, these calves achieved C<sub>max</sub> at 29.5 and 27.0 ± 3.4 h, whereas WM IgG peaked at 13.4 ± 3.4 h. No differences were observed for apparent efficiency of absorption between treatments from 0 to 12 h and 0 to 24 h. Immunoglobulin G area under the curve (AUC) was the same for COL and MX calves over the entire experimental period and from when treatments were fed. The IgG AUC for 0 to 72 h for WM calves was 27.4% lesser than that for COL calves but not different from MX calves. However, the IgG AUC for 12 to 72 h for WM calves differed relative to that for both COL (30.8% less) and MX (19.6% less) calves. Serum IgG concentrations were more persistent when COL (88.2 ± 2.4%) and MX (91.2 ± 2.4%) were fed rather than WM (75.3 ± 2.4%). Prolonged IgG consumption increased serum IgG concentrations, corresponding to the mass of IgG fed, and improved apparent IgG persistency in Holstein bulls. Neonatal calves should be fed at least 62 g of IgG at 12 h after birth to further increase serum IgG concentrations.
HIV-1 IgA specific serum antibodies and disease progression during HIV-1 infection. (2002)

HIV-1 IgA serum antibodies did not predict the progression of the disease. Correlation between HIV-1 IgA antibodies titer, viral load, and CD4 cell counts was not detected.
Detection of Presentation Attacks in Face Recognition Systems with Non-contact Photoplethysmography (2020)

Presentation attack detection (PAD) is a challenging task for biometric identity verification systems based on face recognition. A presentation attack could be done using a 3D mask, a printed static image obtained from a laser or inkjet printer, or a video replay to exploit the vulnerabilities of the target face recognition system. In order to detect presentation attacks, features, which are useful for liveness detection could be used as clues. Non-contact (remote) photoplethysmography (rPPG) methods could be used to estimate physiological signals such as the heart rate, respiratory rate and heart rate variability that indicate liveness features using only a camera recording without any additional hardware. These physiological signals could be used as features to train a machine learning classifier to differentiate fake faces from genuine faces. In this study, we first provide a literature survey of presentation attack detection methods using non-contact photoplethysmography. We also provide an experimental comparison of PAD using different PPG methods on the 3DMAD dataset.
Advanced Ceramics Electronics Application (2008)

Ceramics materials infringe human life from early years of the humankind. Together with the progress of the science and engineering, various materials and technologies have been used. Suitable materials and technologies have been used in the proper applications. This paper deals with ceramics materials classification. Some progressive technologies and their application are presented especially.
Prior Knowledge Representation for Self-Attention Networks (2021)

Self-attention networks (SANs) have shown promising empirical results in various natural language processing tasks. Typically, it gradually learning language knowledge on the whole training dataset in parallel and stacked ways, thereby modeling language representation. In this paper, we propose a simple and general representation method to consider prior knowledge related to language representation from the beginning of training. Also, the proposed method allows SANs to leverage prior knowledge in a universal way compatible with neural networks. Furthermore, we apply it to one prior word frequency knowledge for the monolingual data and other prior translation lexicon knowledge for the bilingual data, respectively, thereby enhancing the language representation. Experimental results on WMT14 English-to-German and WMT17 Chinese-to-English translation tasks demonstrate the effectiveness and universality of the proposed method over a strong Transformer-based baseline.
Editorial: Universal adhesives - to etch or not to etch? (2021)

status: Published
Technical note: Time lag correction of aquatic eddy covariance data measured in the presence of waves (2015)

Abstract. Extracting benthic oxygen fluxes from eddy covariance time series measured in the presence of surface gravity waves requires careful consideration of the temporal alignment of the vertical velocity and the oxygen concentration. Using a model based on linear wave theory and measured eddy covariance data, we show that a substantial error in flux can arise if these two variables are not aligned correctly in time. We refer to this error in flux as the time lag bias. In one example, produced with the wave model, we found that an offset of 0.25 s between the oxygen and the velocity data produced a 2-fold overestimation of the flux. In another example, relying on nighttime data measured over a seagrass meadow, a similar offset reversed the flux from an uptake of −50 mmol m−2 d−1 to a release of 40 mmol m−2 d−1. The bias is most acute for data measured at shallow-water sites with short-period waves and low current velocities. At moderate or higher current velocities (&gt; 5–10 cm s−1), the bias is usually insignificant. The widely used traditional time shift correction for data measured in unidirectional flows, where the maximum numerical flux is sought, should not be applied in the presence of waves because it tends to maximize the time lag bias or give unrealistic flux estimates. Based on wave model predictions and measured data, we propose a new time lag correction that minimizes the time lag bias. The correction requires that the time series of both vertical velocity and oxygen concentration contain a clear periodic wave signal. Because wave motions are often evident in eddy covariance data measured at shallow-water sites, we encourage more work on identifying new time lag corrections.