gebhart/patrepeval huggingface dataset:
Cardiac regenerative potential of adipose tissue-derived stem cells (2009)

Myocyte loss due to ischemia/reperfusion injury leads to cardiac dysfunction and heart failure, and the concentration of current therapy is limited on preventing the progression. Recent interest has focused on transplantation of stem cells to differentiate and replenish the loss of myocytes. Adipose tissue represents an alternative and abundant source of adult stem cells with the ability to differentiate along multiple lineage pathways. Recent studies have demonstrated the potential of adipose tissue-derived stem cells for treatment of acute myocardial infarction. The aim of this review is to discuss the potential therapeutic benefits of adipose tissue-derived stem cells in improving cardiac function post-injury.
Cloning, expression and site-directed mutagenesis of yeast fructose-1,6-bisphosphatase (1987)

In the published version of the above abstract (from the conference Protein Engineering '87), the enzyme was incorrectly printed as ‘fructose-2,6-bisphosphatase’. The correct version appears above.
Contraceptive Efficacy (1995)

Will it work?'' is the question usually asked first and most frequently about any contraceptive method. This question is especially salient for women using teratogenic drugs and their clinicians, particularly dermatologists. As discussed in other articles in this issue of theArchives, dermatologists prescribe two of the most potent teratogenic drugs, isotretinoin (Accutane, Roche Laboratories, Nutley, NJ) and thalidomide.<sup>1,2</sup>Because this question cannot be answered with certainty for any particular couple, most clinicians and counselors try to help patients understand factors influencing contraceptive efficacy.
On the use of the Hilbert transform for processing measured CW data (1992)

The Hilbert transform is a commonly used technique for relating the real and imaginary parts of a causal spectral response. It is found in both continuous and discrete forms and is widely used in circuit analysis, digital signal processing, image reconstruction and remote sensing. One useful application in the area of high-power microwave (HPM) technology is in correcting measured continuous wave (CW) transfer function data, so as to insure causality in reconstructed transient responses. Another application of the Hilbert transform is in the area of complex spectral estimation using magnitude-only data. Here, the applications of the transform to several specific spectral filtering and phase reconstruction problems are illustrated.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Modeling of diamond radiation detectors (2004)

We have built up a computer simulation of the detection mechanism in the diamond radiation detectors. The diamond detectors can be fabricated from a chemical vapour deposition polycrystalline diamond film. In this case, the trapping-detrapping and recombination at the defects inside the grains and at the grain boundaries degrade the transport properties of the material and the charge induction processes. These effects may strongly influence the device’s response. Previous simulations of this kind of phenomena in the diamond detectors have generally been restricted to the simple detector geometries and homogeneous distribution of the defects. In our model, the diamond film (diamond detector) is simulated by a grid. We apply a spatial and time discretization, regulated by the grid resolution, to the equations describing the charge transport and, by using the Shockley-Ramo theorem, we calculate the signal induced on the electrodes. In this way, we can simulate the effects of the nonhomogeneous distributions of the trapping, recombination, or scattering centers and can investigate the differences observed when different particles, energies, and electrode configurations are used. The simulation shows that the efficiency of the detector increases linearly with the average grain size, that the charge collection distance is small compared to the dimensions of a single grain, and that for small grains, the trapping at the intragrain defects is insignificant compared to the effect of the grain boundaries.
An Overview of Clinical Trial Data on a New Formulation of Botulinum Neurotoxin Type A (2009)

According to the American Society for Aesthetic Plastic Surgery, the number of aesthetic procedures involving botulinum neurotoxin type A (BoNT-A) increased 3681% between 1997 and 2008. Almost 2.5 million procedures were performed using this neurotoxin last year, accounting for approximately 25% of all nonsurgical aesthetic procedures performed in 2008.1 Both aesthetic and therapeutic uses of this neurotoxin are likely to increase in the coming years.

In 2009, a new US formulation of BoNT-A (BoNTA-ABO; Dysport [abobotulinumtoxinA]; Medicis Aesthetics, Scottsdale, AZ) was approved by the US Food and Drug Administration (FDA) for the treatment of moderate to severe glabellar lines. Key features of this new BoNT-A formulation are shown in the Table.2 Outside of North America, Dysport has been used for therapeutic applications since 1991 and for aesthetic applications since 2001. Before receiving FDA approval, BoNTA-ABO had been studied in the United States under the proposed trade name Reloxin . At the time of approval, the FDA required Medicis to retain the Dysport name.

View this table:

Table 
Characteristics of Dysport in the treatment of moderate to severe glabellar lines2



Several reports of pivotal US trial data regarding the safety and efficacy of this agent in patients with moderate to severe glabellar lines have been published recently in major journals.3–7 This supplement provides an up-to-date and comprehensive overview of the safety, efficacy, immunogenicity, and manufacturing of this new US botulinum neurotoxin formulation.

Food poisoning by Clostridium botulinum neurotoxin has probably been a cause of death since humans first began storing food.8 C botulinum is a Gram-positive, anaerobic bacteria that is categorized on the basis of the toxins it secretes. Of the eight known …
Microstructure and Optical Properties of Oxygen-Annealed c-Si/a-SiO2 Core–Shell Silicon Nanowires (2012)

Core–shell silicon nanowires (Si-NWs) were synthesized by chemical vapor deposition (CVD) using SiH4 and H2 as precursors. The electron microscopy results revealed that the core shell Si-NW consists of core Si surrounded by thick amorphous silicon oxide (a-SiOx). The diameter of core Si in a core–shell Si-NW was controlled by varying the oxygen flow rate and annealing time. The morphology of core shell Si-NWs is changed to silicon nanocoils (Si-NCs)/silicon nanonoodles (Si-NNs) after being annealed in oxygen atmosphere. The Fourier transform infrared spectroscopy (FTIR) study confirmed the changes of Si–Si and Si–O–Si vibration band and the intensity of Si–O–Si vibration mode increases with decreasing diameter of Si-NWs. The optical properties of core–shell Si-NWs are improved after being annealed in oxygen atmosphere. The chemical bonding and photoluminescence properties of oxygen annealed Si-NWs are also discussed in this article.
Highly-efficient terahertz emission from hydrogen-bonded single molecular crystal 4-nitro-2,5-bis(phenylethynyl)aniline (2021)

We report terahertz electromagnetic wave emission by optical rectification from hydrogen-bonded single molecular crystal 4-nitro-2,5-bis(phenylethynyl)aniline designed to be polar via the hydrogen bonding between nitroaniline cores. The terahertz emission efficiency is comparable to the representative inorganic terahertz emitter ZnTe. We show terahertz emission characteristics, optical spectrum, and theoretical molecular orbital calculations. Another three kinds of nitroaniline-based organic molecules are revealed to form polar crystal structure, and they have large hyperpolarizabilities and have potential for terahertz photonics.
Capturing a Musician's Groove: generation of realistic accompaniments from single song recordings (2015)

This demonstration presents a concatenative synthesis engine for the generation of musical accompaniments, based on chord progressions. The system takes a player's song recording as input, and generates the accompaniment for any other song, based on the input content. We show that working on accompaniment requires a special care about temporal deviations at the border of the sliced chunks, because they make most of the rhythmic groove. We address it by discriminating accidental deviations against intentional ones, in order to correct the first while keeping the second. We will provide a full demonstration of the system, from the recording process to the generation, in various conditions, inviting the audience to participate.
Selection of antibodies to cell surface determinants on mouse thymic epithelial cells using a phage display library (1997)

The network of thymic epithelium contributes significantly to the thymic stromal cell environment, which plays a vital role in the generation and maturation of thymocytes. Monoclonal antibodies (mAb) have revealed considerable heterogeneity within this epithelial component of the mouse thymic microenvironment, but many of these antibodies recognize epitopes that are located inside the cell and so cannot be used in functional studies. As an alternative approach to isolate antibodies specific to thymic epithelium, we used a phage display library expressing single chain Fv antibodies. For selection, a thymic cell suspension was incubated with the phage display library, and major histocompatibility complex class II positive cells, the majority of which are epithelial, were then specifically selected. Phage bound to these cells were eluted and the selection procedure was repeated for a further five rounds. Immunohistochemical analysis revealed that these phage antibodies show differential staining of thymic epithelial subsets. Flow cytometric analysis of a thymic epithelial cell line using a panel of these antibodies demonstrated that they recognize epitopes on the cell surface. Furthermore, some of these antibodies also labelled human thymic epithelium, suggesting that the epitopes recognized by these antibodies are conserved between human and rodent thymus. Our approach therefore provides a rapid method to select antibodies specific for thymic epithelial cell surface determinants in their native configuration.
The 5′ flanking region of the gene for the Epstein-Barr virus-encoded nuclear antigen 2 contains a cell type specificcis-acting regulatory element that activates transcription in transfected B-cells (1988)

We have recently identified the promoter that positions the initiation (cap) site for RNA encoding the Epstein-Barr virus (EBV) determined nuclear antigen 2 (EBNA2) in transfected COS-1 cells. The cells were transfected with recombinant vectors that contained the BamHI WYH region of the EBV genome. In order to delineate regulatory DNA sequences required for the expression of EBNA2 the 5' flanking region of the gene was linked to reporter genes in expression vectors and transfected into EBV genome-negative lymphoid DG75 cells. We demonstrate that several cis-acting elements contribute to a transcriptional enhancer activity found in the region between nucleotides-553 and -86 relative to the cap site. The enhancer was active in lymphoid DG75 cells but not in HeLa cells and stimulated transcription also from the heterologous thymidine kinase (TK) and beta-globin promoters. Nuclear extracts of lymphoid cells contained protein factors that bound to the enhancer. The in vitro introduction of a mutation in the enhancer sequence that substantially reduced the transcription stimulatory activity concurrently blocked the binding of one of the factors.
Functionalized Deep-Cavity Cavitands (1999)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTFunctionalized Deep-Cavity CavitandsHuaping Xi, Corinne L. D. Gibb, and Bruce C. GibbView Author Information Department of Chemistry, University of New Orleans, New Orleans, Louisiana 70148 Cite this: J. Org. Chem. 1999, 64, 25, 9286–9288Publication Date (Web):November 17, 1999Publication History Received22 June 1999Published online17 November 1999Published inissue 1 December 1999https://pubs.acs.org/doi/10.1021/jo9909913https://doi.org/10.1021/jo9909913brief-reportACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views2009Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Anions,Functional groups,Materials,Reaction products,Scaffolds Get e-Alerts
Participant-Partners in Genetic Research: An Exome Study with Families of Children with Unexplained Medical Conditions (Preprint) (2017)

<sec> <title>BACKGROUND</title> Unlike aggregate research on groups of participants with a particular disorder, genomic research on discrete families’ rare conditions could result in data of use to families, their healthcare, as well as generating knowledge on the human genome. </sec> <sec> <title>OBJECTIVE</title> In a study of families seeking to rule in/out genetic causes for their children’s medical conditions via exome sequencing, we solicited their views on the importance of genomic information. Our aim was to learn the interests of parents in seeking genomic research data and to gauge their responsiveness and engagement with the research team. </sec> <sec> <title>METHODS</title> At enrollment, we offered participants options in the consent form for receiving potentially clinically relevant research results. We also offered an option of being a “partner” versus a “traditional” participant; partners could be re-contacted for research and study activities. We invited adult partners to complete a pre-exome survey, attend annual family forums, and participate in other inter-family interaction opportunities. </sec> <sec> <title>RESULTS</title> Of the 385 adults enrolled, 79% opted for “partnership” with the research team. Nearly all (99.2%) participants opted to receive research results pertaining to their children’s primary conditions. A majority indicated the desire to receive additional clinically relevant outside the scope of their children’s conditions (92.7%) and an interest in non-clinically relevant genetic information (82.7%). </sec> <sec> <title>CONCLUSIONS</title> Most participants chose partnership, including its rights and potential burdens; however, active engagement in study activities remained the exception. Not surprisingly, the overwhelming majority of participants—both partners and traditional—expected to receive all genetic information resulting from the research study. </sec>
Atomic layer deposition of BN as a novel capping barrier for B2O3 (2019)

The deposition of boron oxide (B2O3) films on Si and SiO2 substrates by atomic layer deposition (ALD) is of growing interest in microelectronics for shallow doping of high aspect ratio transistor structures. B2O3, however, forms volatile boric acid (H3BO3) upon ambient exposure, requiring a passivation barrier, for which BN was investigated as a possible candidate. Here, the authors demonstrate in situ deposition of BN by sequential BCl3/NH3 reactions at 600 K on two different oxidized boron substrates: (a) B2O3 deposited using BCl3/H2O ALD on Si at 300 K (“B2O3/Si”) and (b) a boron-silicon oxide formed by sequential BCl3/O2 reactions at 650 K on SiO2 followed by annealing to 1000 K (“B-Si-oxide”). X-ray photoelectron spectroscopy (XPS) data demonstrate layer-by-layer growth of BN on B2O3/Si with an average growth rate of ∼1.4 Å/cycle, accompanied by some B2O3 removal during the first BN cycle. In contrast, continuous BN growth was observed on B-Si-oxide without any reaction with the substrate. XPS data also indicate that the oxide/nitride heterostructures are stable upon annealing in ultrahigh vacuum to &amp;gt;1000 K. XPS data, after the exposure of these heterostructures to ambient, indicate a small amount of BN oxidation at the surface NHx species, with no observable hydroxylation of the underlying oxide films. These results demonstrate that BN films, as thin as 13 Å, are potential candidates for passivating boron oxide films prepared for shallow doping applications.
Signaling Mechanisms Controlling Cell Fate and Embryonic Patterning (2012)

During development, signaling pathways specify cell fates by activating transcriptional programs in response to extracellular signals. Extensive studies in the past 30 years have revealed that surprisingly few pathways exist to regulate developmental programs and that dysregulation of these can lead to human diseases, including cancer. Although these pathways use distinct signaling components and signaling strategies, a number of common themes have emerged regarding their organization and regulation in time and space. Examples from Drosophila, such as Notch, Hedgehog, Wingless/WNT, BMP (bone morphogenetic proteins), EGF (epidermal growth factor), and FGF (fibroblast growth factor) signaling, illustrate their abilities to act either at a short range or over a long distance, and in some instances to generate morphogen gradients that pattern fields of cells in a concentration-dependent manner. They also show how feedback loops and transcriptional cascades are part of the logic of developmental regulation.
Impact of Pacemaker Failover Configuration on Mean Time to Recovery for Small Cloud Clusters (2014)

In cloud environments High Availability characteristics are established by the usage of failover software (like e.g. HAProxy, Keepalive or Pacemaker). Though these tools enable automatic recovery of cloud services from outages, the recovery can still be very slow if it is not configured adequately. In this paper we developed a "Recovery Time Test" to determine if recovery time depends on configuration of the failover software and how recovery time depends on configuration settings. Another goal of the Recovery Time Test is to determine the factor by which recovery time can be decreased by a given configuration. As proof of concept, we applied the Recovery Time Test to an OpenStack cloud environment which is controlled by the Pacemaker failover software. Pacemaker mean recovery time can take a value between 110 and 160 seconds, if the tool is configured badly. We found that with a proper configuration Pacemaker mean recovery time can be reduced significantly to a value between 15 and 20 seconds.
Synthesis of Vitamin D (Calciferol) (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Vitamin D (Calciferol)Gui-Dong Zhu and William H. OkamuraCite this: Chem. Rev. 1995, 95, 6, 1877–1952Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/cr00038a007https://doi.org/10.1021/cr00038a007research-articleACS PublicationsRequest reuse permissionsArticle Views5319Altmetric-Citations301LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Association of the 1q25 diabetes-specific coronary heart disease locus with alterations of the γ-glutamyl cycle and increased methylglyoxal levels in endothelial cells (2020)

A chromosome 1q25 variant (rs10911021) has been associated with coronary heart disease (CHD) in type 2 diabetes (T2D). In human umbilical vein endothelial cells (HUVECs), the risk allele ‘C’ is associated with lower expression of the adjacent gene &lt;i&gt;GLUL&lt;/i&gt; encoding glutamine synthase, converting glutamic acid to glutamine. To further investigate the mechanisms through which this locus affects CHD risk, we measured 35 intracellular metabolites involved in glutamic acid metabolism and g-glutamyl cycle in 62 HUVEC strains carrying different rs10911021 genotypes. Eight metabolites were positively associated with the risk allele (17%-58% increase/allele copy, p=0.046-0.002), including five g-glutamyl amino acids, b-citryl-glutamate, N-acetyl-aspartyl-glutamate, and ophthalmate - a marker of g-glutamyl cycle malfunction. Consistent with these findings, the risk allele was also associated with decreased glutathione/glutamate ratio (-9%, p=0.012), decreased S-lactoylglutathione (-41%, p=0.019), and reduced detoxification of the atherogenic compound methylglyoxal (+54%, p=0.008). &lt;i&gt;GLUL&lt;/i&gt; down-regulation by shRNA caused a 40% increase in methylglyoxal level, which was completely prevented by glutamine supplementation. In summary, we have identified intracellular metabolic traits associated with the 1q25 risk allele in HUVECs, including impairments of the g-glutamyl cycle and methylglyoxal detoxification. Glutamine supplementation abolishes the latter abnormality, suggesting that such treatment may prevent CHD in 1q25 risk allele carriers.