gebhart/patrepeval huggingface dataset:
Rapid screening of MMACHC gene mutations by high‐resolution melting curve analysis (2020)

The established PCR-HRM method for screening common pathogenic MMACHC variants in Chinese patients with cblC has the advantages of high accuracy, high throughput, low cost, and high speed. It is suitable for the large-sample screening of suspected children with methylmalonic acidemia and carriers in population.
Patterning Microsphere Surfaces by Templating Colloidal Crystals (2004)

By using the upper single or double layers in colloidal crystals as masks during Au vapor deposition, various Au patterns have been successfully constructed on the surfaces of the lower spheres. The dimension and geometry of the Au patterns obtained are dependent on the orientation of the colloidal crystal templates. Our patterning procedure is independent of the curvature and chemical composition of the surfaces, which definitely pave a promising way to pattern highly curved surfaces.
Dimensional Analysis of Dynamic MEMS Micro-Robotic Walking Subject to Orthogonal Actuation and Small-Scale Forces (2013)

This research evaluates simulated walking micro-robots based on orthogonal lateral and vertical actuators, a common restriction on micro-electromechanical system (MEMS) actuation, and subject to small damping effects and certain small-scale external forces operating on robot feet. Dimensional analysis is used to identify scalable trends in a numerical simulation of a simple walking dynamics model in which lateral and vertical motion are treated as dynamically-uncoupled, the limiting case for orthogonal MEMS actuators. The results of this analysis are that the system damping significantly affects walking speed, while small-scale foot-ground interaction forces other than adhesion have significant effects only over large order-of-magnitude variation in robot size. Results are intended to serve as a design tool for walking micro-robots.
Adaptive loss‐less data compression method optimized for GPU decompression (2017)

Summary There is no doubt that data compression is very important in computer engineering. However, most lossless data compression and decompression algorithms are very hard to parallelize, because they use dictionaries updated sequentially. The main contribution of this paper is to present a new lossless data compression method that we call adaptive loss‐less (ALL) data compression. It is designed so that the data compression ratio is moderate, but decompression can be performed very efficiently on the graphics processing unit (GPU). This makes sense for applications such as training of deep learning, in which compressed archived data are decompressed many times. To show the potentiality of ALL data compression method, we have evaluated the running time using five images and five text data and compared ALL with previously published lossless data compression methods implemented in the GPU, Gompresso, CULZSS, and LZW. The data compression ratio of ALL data compression is better than the others for eight data out of these 10 data. Also, our GPU implementation on GeForce GTX 1080 GPU for ALL decompression runs 84.0 to 231 times faster than the CPU implementation on Core i7‐4790 CPU. Further, it runs 1.22 to 23.5 times faster than Gompresso, CULZSS, and LZW running on the same GPU.
Enhanced protective responses to a serotype-independent pneumococcal vaccine when combined with an inactivated influenza vaccine (2016)

Streptococcus pneumoniae and influenza are the world's foremost bacterial and viral respiratory pathogens. We have previously described a γ-irradiated influenza A virus (γ-FLU) vaccine that provides cross-protective immunity against heterosubtypic infections. More recently, we reported a novel non-adjuvanted γ-irradiated S pneumoniae (γ-PN) vaccine that elicits serotype-independent protection. Considering the clinical synergism of both pathogens, combination of a serotype-independent pneumococcal vaccine with a broad-spectrum influenza vaccine to protect against both infections would have a considerable clinical impact. In the present study, we co-immunized C57BL/6 mice intranasally (IN) with a mixture of γ-PN (whole inactivated cells) and γ-FLU (whole inactivated virions) and examined protective efficacy. Co-immunization enhanced γ-PN vaccine efficacy against virulent pneumococcal challenge, which was dependent on CD4<sup>+</sup> T-cell responses. In contrast, vaccination with γ-PN alone, co-immunization enhanced pneumococcal-specific effector T-helper 17 cell (Th17) and Th1 memory cell, promoted development of CD4<sup>+</sup> tissue-resident memory (TRM) cells and enhanced Pneumococcus-specific antibody responses. Furthermore, co-immunization elicited significant protection against lethal influenza challenge, as well as against co-infection with both influenza and S pneumoniae. This is the first report showing the synergistic effect of combining whole cell and whole virion vaccines to both S pneumoniae and influenza as a single vaccine to protect against individual and co-infection, without compromising pathogen-specific immunity.
The <i>P. c.</i> subsp. <i>carotovorum budAB</i> operon affects bacterial modification of pH in tubers slices that are incubated anaerobically. (2015)

&lt;p&gt;Potato slices were inoculated with 10&lt;sup&gt;6&lt;/sup&gt; CFU and incubated under aerobic (&lt;b&gt;A&lt;/b&gt;) and anaerobic conditions (&lt;b&gt;B&lt;/b&gt;) for 20 hours at room temperature (∼22°C). &lt;b&gt;PR&lt;/b&gt;: pH indicator phenol red (yellow at pH below 6.4 and red violet at pH above 8.2), &lt;b&gt;BP&lt;/b&gt;: pH indicator bromocresol purple (yellow at pH below 5.2 and purple at a pH above 6.8).&lt;/p&gt;
SENSEI traffic impact on mobile wireless networks (2010)

With an increasing number of connected devices and the coming wave of Internet of Things services, it is important to understand how the modern communication networks will cope with the additional traffic load. In this paper, an analysis of several Internet of things scenarios as envisioned by the FP7 SENSEI project have been performed to understand how the traffic is being generated in each of the scenarios. Based on the analysis, a traffic model is derived and then used to assess its impact on wireless mobile networks from the networks' dimensioning point of view. The results of this analysis are then presented.
Arabidopsis Lectin Receptor Kinases LecRK-IX.1 and LecRK-IX.2 Are Functional Analogs in Regulating Phytophthora Resistance and Plant Cell Death (2015)

L-type lectin receptor kinases (LecRK) are potential immune receptors. Here, we characterized two closely-related Arabidopsis LecRK, LecRK-IX.1 and LecRK-IX.2, of which T-DNA insertion mutants showed compromised resistance to Phytophthora brassicae and Phytophthora capsici, with double mutants showing additive susceptibility. Overexpression of LecRK-IX.1 or LecRK-IX.2 in Arabidopsis and transient expression in Nicotiana benthamiana increased Phytophthora resistance but also induced cell death. Phytophthora resistance required both the lectin domain and kinase activity, but for cell death, the lectin domain was not needed. Silencing of the two closely related mitogen-activated protein kinase genes NbSIPK and NbNTF4 in N. benthamiana completely abolished LecRK-IX.1-induced cell death but not Phytophthora resistance. Liquid chromatography-mass spectrometry analysis of protein complexes coimmunoprecipitated in planta with LecRK-IX.1 or LecRK-IX.2 as bait, resulted in the identification of the N. benthamiana ABC transporter NbPDR1 as a potential interactor of both LecRK. The closest homolog of NbPDR1 in Arabidopsis is ABCG40, and coimmunoprecipitation experiments showed that ABCG40 associates with LecRK-IX.1 and LecRK-IX.2 in planta. Similar to the LecRK mutants, ABCG40 mutants showed compromised Phytophthora resistance. This study shows that LecRK-IX.1 and LecRK-IX.2 are Phytophthora resistance components that function independent of each other and independent of the cell-death phenotype. They both interact with the same ABC transporter, suggesting that they exploit similar signal transduction pathways.
Counting Boundary Paths for Oriented Percolation Clusters (1999)

We shall use analytic methods to prove bounds on the analogue of the connective constant for the oriented percolation on ℤ2. ©1999 John Wiley & Sons, Inc. Random Struct. Alg., 14, 1–28, 1999
Phospholipase A2IVα Regulates Phagocytosis Independent of Its Enzymatic Activity (2012)

Group IVα phospholipase A(2) (PLA(2)IVα) is a lipolytic enzyme that catalyzes the hydrolysis of membrane phospholipids to generate precursors of potent inflammatory lipid mediators. Here, the role of PLA(2)IVα in Fc receptor (FcR)-mediated phagocytosis was investigated, demonstrating that PLA(2)IVα is selectively activated upon FcR-mediated phagocytosis in macrophages and that it rapidly translocates to the site of the nascent phagosome. Moreover, pharmacological inhibition of PLA(2)IVα by pyrrophenone reduces particle internalization by up to 50%. In parallel, fibroblasts from PLA(2)IVα knock-out mice overexpressing FcγRIIA and able to internalize IgG-opsonized beads show 50% lower phagocytosis, compared with wild-type cells, and transfection of PLA(2)IVα fully recovers this impaired function. Interestingly, transfection of the catalytically inactive deleted PLA(2)IVα mutant (PLA(2)IVα(1-525)) and point mutant (PLA(2)IVα-S228C) also promotes recovery of this impaired function. Finally, transfection of the PLA(2)IVα C2 domain (which is directly involved in PLA(2)IVα membrane binding), but not of PLA(2)IVα-D43N (which cannot bind to membranes), rescues FcR-mediated phagocytosis. These data unveil a new mechanism of action for PLA(2)IVα, which demonstrates that the membrane binding, and not the enzymatic activity, is required for PLA(2)IVα modulation of FcR-mediated phagocytosis.
Electrical field impact on the gas adsorptivity of thin metal oxide films (1998)

A thin semiconducting NiO film is exposed to NO2 at room temperature. This exposure causes a work function change at the surface of the film due to adsorption of the NO2 molecules. It is found that there is a strong dependence of the adsorptivity, i.e., the amount of work function change per unit time, on the magnitude of an electrical field that is applied perpendicular to the film surface. This induced adsorptivity change is known as the electroadsorptive effect. In order to modulate the adsorptivity significantly, the electrical field strength must exceed 104 V/cm. This requirement can be achieved by using hybrid suspended gate field effect transistors with an air gap height below 1 μm.
A site-specific endodeoxyribonuclease from Streptomyces albus CMI 52766 sharing site-specificity with Providencia stuartii endonuclease

Pst

l (1977)

A class II site-specific endodeoxyribonuclease (SalPI) was identified in cell-free extracts of Streptomyces albus CMI 52766 after high speed centrifugation and fractionation through Bio Gel AO.5M. SalPI cleaves lambda DNA into at least 18 fragments. Five cleavage sites were located in the linear lambda map by the use of double and triple restriction enzyme digests involving EcoRI, HindIII, SalGI and another new Streptomyces enzyme, SacI. The results were indistinguishable from those previously obtained for a Providencia stuartii enzyme, PstI, by Smith, Blattner & Davies (Nucleic Acids Res. 1976 3, 343). SalPI and PstI were shown by a double digest test to have the same site specificity. None of 34 phages tested was obviously restricted by S. albus CMI 52766, and correspondingly DNA from two of them was not cleaved in vitro by SalPI. DNA from Streptomyces phage that does not form plaques on S. albus CMI 52766, and plasmid SCP2 DNA from S. coelicolor A3 (2), were both cleaved.
Overview of the epidemiology of colorectal cancer (1997)

Conclusive evidence of the effectiveness of primary prevention of colorectal cancer via dietary measures or nonsteroidal anti-inflammatory drugs is lacking. Secondary prevention by interrupting the adenoma carcinoma sequence is an actual possibility, its effectiveness, however, needs to be determined. Molecular genetics holds a promise for identifying populations at high risk for colorectal cancer, therefore, targeting the screening to make it more cost-effective.
Antibody Repertoire and Gene Expression Profile: Implications for Different Developmental and Functional Traits of Splenic and Peritoneal B-1 Lymphocytes (2003)

In L2 mice, a high expression level of the transgenic lambda2(315) L chain results in nearly complete exclusion of endogenous L chains and a predominance of B-1a cells. In this study, we show that splenic and peritoneal B-1a cells differ considerably in their Ab repertoire and gene expression profile. Splenic B-1a cells exhibit a more diversified repertoire under L chain limitation. Despite oligoclonal overlaps between both B-1a compartments, some B cell receptor specificities are clearly restricted to the peritoneum. The capacity of peritoneal B-1a cells to enter the splenic B-1a compartment was found to be very limited. Gene expression profiling revealed genes up-regulated in splenic B-1a cells that are involved in mediating specialized first-line-of-defense effector functions and interaction with T cells. Thus, splenic and peritoneal B-1a cells differ not only in their developmental program but also in functional properties.
Microstructural Modification of AZ91 Magnesium Alloy Using Friction Stir Processing and Carbon Fibers (2017)

The microstructure of cast magnesium (Mg) alloy, AZ91, was modified by a friction stir process (FSP) technique. FSP was applied to AZ91 plate with a narrow slit, in which carbon fibers (CFs) were filled. The rotating FSP tool consisting of probe and shoulder could modify the microstructure of the material by sever plastic deformation (SPD) and simultaneously disperse CFs into the matrix. Microstructural observation revealed that sever stirring of material by a tool resulted in the grain refinement due to the dynamic recrystallization and distribution of CFs into the stir zone (SZ). The distribution was not uniform depending on the plastic flow in the SZ, but any worm-hole defects were not formed. Hardness of FSPed AZ91 in the SZ was higher than the as-casted AZ91 because of the grain refinement and distribution of hard CFs.
β‐ Alanine, a Possible Neurotransmitter in the Visual System? (1981)

The chemically evoked efflux of endogenous amino acids from perfused rabbit superior colliculus (SC) slices was studied. Amino acids in the perfusates were determined fluorimetrically with a precolumn derivatisation method and subsequent separation on an HPLC column. Potassium-induced depolarisation caused a calcium-dependent release of beta-alanine, GABA, glutamate, and aspartate. Veratridine-evoked efflux was essentially similar in selectivity and could be blocked by tetrodotoxin (TTX). The results are indicative of a neurotransmitter role of beta-alanine, GABA, glutamate, and aspartate in the SC of the rabbit.
Supplementary Figure Legends 1-7 from KLF6-SV1 Is a Novel Antiapoptotic Protein That Targets the BH3-Only Protein NOXA for Degradation and Whose Inhibition Extends Survival in an Ovarian Cancer Model (2023)

Supplementary Figure Legends 1-7 from KLF6-SV1 Is a Novel Antiapoptotic Protein That Targets the BH3-Only Protein NOXA for Degradation and Whose Inhibition Extends Survival in an Ovarian Cancer Model
Syngas Derived from Lignocellulosic Biomass Gasification as an Alternative Resource for Innovative Bioprocesses (2020)

A hybrid system based on lignocellulosic biomass gasification and syngas fermentation represents a second-generation biorefinery approach that is currently in the development phase. Lignocellulosic biomass can be gasified to produce syngas, which is a gas mixture consisting mainly of H2, CO, and CO2. The major challenge of biomass gasification is the syngas’s final quality. Consequently, the development of effective syngas clean-up technologies has gained increased interest in recent years. Furthermore, the bioconversion of syngas components has been intensively studied using acetogenic bacteria and their Wood–Ljungdahl pathway to produce, among others, acetate, ethanol, butyrate, butanol, caproate, hexanol, 2,3-butanediol, and lactate. Nowadays, syngas fermentation appears to be a promising alternative for producing commodity chemicals in comparison to fossil-based processes. Research studies on syngas fermentation have been focused on process design and optimization, investigating the medium composition, operating parameters, and bioreactor design. Moreover, metabolic engineering efforts have been made to develop genetically modified strains with improved production. In 2018, for the first time, a syngas fermentation pilot plant from biomass gasification was built by LanzaTech Inc. in cooperation with Aemetis, Inc. Future research will focus on coupling syngas fermentation with additional bioprocesses and/or on identifying new non-acetogenic microorganisms to produce high-value chemicals beyond acetate and ethanol.
Comparison of Meniscal Cell-Mediated and Chondrocyte-Mediated Calcification (2017)

These findings suggest that OA meniscal cells have a similar calcifying potential as OA chondrocytes, supporting a pathogenic role of OA menisci in OA.
Aberrant DNA methylation of WNT pathway genes in the development and progression of CIMP-negative colorectal cancer (2016)

The WNT signaling pathway has an essential role in colorectal carcinogenesis and progression, which involves a cascade of genetic and epigenetic changes. We aimed to analyze DNA methylation affecting the WNT pathway genes in colorectal carcinogenesis in promoter and gene body regions using whole methylome analysis in 9 colorectal cancer, 15 adenoma, and 6 normal tumor adjacent tissue (NAT) samples by methyl capture sequencing. Functional methylation was confirmed on 5-aza-2'-deoxycytidine-treated colorectal cancer cell line datasets. In parallel with the DNA methylation analysis, mutations of WNT pathway genes (APC, β-catenin/CTNNB1) were analyzed by 454 sequencing on GS Junior platform. Most differentially methylated CpG sites were localized in gene body regions (95% of WNT pathway genes). In the promoter regions, 33 of the 160 analyzed WNT pathway genes were differentially methylated in colorectal cancer vs. normal, including hypermethylated AXIN2, CHP1, PRICKLE1, SFRP1, SFRP2, SOX17, and hypomethylated CACYBP, CTNNB1, MYC; 44 genes in adenoma vs. NAT; and 41 genes in colorectal cancer vs. adenoma comparisons. Hypermethylation of AXIN2, DKK1, VANGL1, and WNT5A gene promoters was higher, while those of SOX17, PRICKLE1, DAAM2, and MYC was lower in colon carcinoma compared to adenoma. Inverse correlation between expression and methylation was confirmed in 23 genes, including APC, CHP1, PRICKLE1, PSEN1, and SFRP1. Differential methylation affected both canonical and noncanonical WNT pathway genes in colorectal normal-adenoma-carcinoma sequence. Aberrant DNA methylation appears already in adenomas as an early event of colorectal carcinogenesis.
Solubility of hydrogen in alcohols and esters (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolubility of hydrogen in alcohols and estersMark S. Wainwright, Thomas Ahn, David L. Trimm, and Noel W. CantCite this: J. Chem. Eng. Data 1987, 32, 1, 22–24Publication Date (Print):January 1, 1987Publication History Published online1 May 2002Published inissue 1 January 1987https://pubs.acs.org/doi/10.1021/je00047a006https://doi.org/10.1021/je00047a006research-articleACS PublicationsRequest reuse permissionsArticle Views1767Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Role of Crystallinity in Retention of Polymer Particle Morphology in the Presence of Compressed Carbon Dioxide (1999)

The effects of compressed carbon dioxide absorption into poly(l-lactide), poly(dl-lactide), poly(glycolide), and their copolymers were observed using EPR and mechanical creep experiments to determine the correlation between the glass transition pressure and material failure pressure. Results show polymers can retain particle morphology above the carbon dioxide suppressed glass transition pressure because of a moderate crystalline volume fraction that reduces creep rates enough to limit particle agglomeration during precipitation with a compressed antisolvent.
24. The Clinical Development of Lumateperone (ITI-007) for the Treatment of Schizophrenia (2017)

Background: Schizophrenia affects about 1% of the population and exerts a great burden on patients and caregivers. Newer treatments are needed to provide broad symptom control and improved tolerability. Lumateperone is an investigational agent in late-stage development for schizophrenia, bipolar depression and agitation associated with dementia. Through synergistic actions via serotonergic, dopaminergic and glutamatergic systems, lumateperone represents a novel therapeutic approach. Methods: The late-stage schizophrenia program comprised three well-controlled trials in patients during an acute exacerbation. ITI-007-005 included 335 patients randomized to receive either lumateperone (60 mg or 120 mg), risperidone (positive control) or placebo for 4 weeks. ITI-007-301 included 450 patients randomized to receive either lumateperone (60 mg or 40 mg) or placebo for 4 weeks. ITI-007-302 included 696 patients randomized to receive either lumateperone (60 mg or 20 mg), risperidone (positive control) or placebo for 6 weeks. The primary efficacy end point in all studies was PANSS total score change from baseline versus placebo. Results: In studies ITI-007-005 and ITI-007-301, lumateperone 60 mg met the primary end point and demonstrated efficacy with statistically significant superiority over placebo at Day 28. Lumateperone 60 mg, which requires no dose titration, also showed early efficacy (week 1) on both the PANSS total score and the PANSS Positive Symptom subscale score in ITI-007-301, and maintained efficacy throughout the study. In ITI-007-301, lumateperone 60 mg also met the key secondary endpoint of statistically significant improvement on the CGI-S. In this study, lumateperone 40 mg separated significantly from placebo on the PANSS positive subscale and the CGI-S, but not on the primary endpoint. Pro-social benefits were also observed with lumateperone in both studies. In ITI-007-302, neither dose of lumateperone separated from placebo on the primary endpoint in the pre-defined patient population. Risperidone did separate from placebo. While the same magnitude and trajectory of improvement with lumateperone 60 mg was observed in all 3 studies (005, 301 and 302), an unusually high placebo response was observed in this last study. Post-hoc analyses revealed improved treatment effects for lumateperone when placebo response was controlled for. Across all studies, lumateperone was well tolerated and exhibited a safety profile similar to placebo. In 2 studies that included risperidone as an active control, lumateperone demonstrated statistically significant advantages over risperidone on key safety and tolerability parameters. Conclusion: Lumateperone represents a new approach to the treatment of schizophrenia with unique pharmacology and a differentiating clinical profile.