805 THE MATRICELLULAR PROTEIN HEVIN IS INCREASED IN THE MAJOR PELVIC GANGLION FOLLOWING CAVERNOUS NERVE INJURY AND INHIBITS NEURITE OUTGROWTH (2012)

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2012805 THE MATRICELLULAR PROTEIN HEVIN IS INCREASED IN THE MAJOR PELVIC GANGLION FOLLOWING CAVERNOUS NERVE INJURY AND INHIBITS NEURITE OUTGROWTH Paula J. Hurley, Omer Kutlu, Johanna L. Hannan, Xiaopu Liu, Arthur L. Burnett, Edward M. Schaeffer, and Trinity J. Bivalacqua Paula J. HurleyPaula J. Hurley Baltimore, MD , Omer KutluOmer Kutlu Baltimore, MD , Johanna L. HannanJohanna L. Hannan Baltimore, MD , Xiaopu LiuXiaopu Liu Baltimore, MD , Arthur L. BurnettArthur L. Burnett Baltimore, MD , Edward M. SchaefferEdward M. Schaeffer Baltimore, MD , and Trinity J. BivalacquaTrinity J. Bivalacqua Baltimore, MD View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.894AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Hevin, a member of the secreted protein, acidic and rich cysteine family of matricellular proteins is increased in neurodegenerative diseases. We hypothesize that Hevin may be a component of the matrix response involved in remodeling events associated with neuronal degeneration to injury. The purpose of this study was to: 1) characterize the changes in Hevin in the major pelvic ganglion (MPG) after cavernous nerve injury (CNI), and 2) determine the effect of Hevin on MPG ex vivo neurite outgrowth. METHODS Two groups of rats (8-10 wks) were utilized: 1) sham and 2) bilateral CNI. 14 days after CNI MPGs were assessed for immunolocalization of Hevin and Western blots were also performed. Human prostatectomy specimens were stained for Hevin to determine presence in cavernous nerves. In a separate series of experiments, the dorsocaudal region of the MPG was isolated and grown in growth factor enriched matrigel. MPG's were treated with BDNF, VEGF, or both then neurite outgrowth was determined in the absence or presence of recombinant mouse Hevin (10 μg/ml). RESULTS Hevin immunoreactivity was localized to ganglion cells and nerve fibers with increased (p<0.05) expression after CNI. Western blot analysis showed a significant increase in Hevin expression in MPG following CNI. Human cavernous nerve fibers and ganglion cell bodies were positive for Hevin. Growth factor treatment of MPG ex vivo resulted in neurite outgrowth which was inhibited by recombinant Hevin. There was significantly less (p<0.05) neuritogenesis (total fiber length and area) from the MPG in the presence of Hevin. CONCLUSIONS These data suggest that Hevin can inhibit MPG neurite growth and thus upregulation of Hevin in response to CNI in the MPG may contribute to cavernous neuronal degeneration. An understanding of the detrimental effects of Hevin signaling in the MPG after injury and its influence on physiology of penile nerve function/repair may advance our understanding of post-radical prostatectomy erectile dysfunction. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e329 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Paula J. Hurley Baltimore, MD More articles by this author Omer Kutlu Baltimore, MD More articles by this author Johanna L. Hannan Baltimore, MD More articles by this author Xiaopu Liu Baltimore, MD More articles by this author Arthur L. Burnett Baltimore, MD More articles by this author Edward M. Schaeffer Baltimore, MD More articles by this author Trinity J. Bivalacqua Baltimore, MD More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...