gebhart/patrepeval huggingface dataset:
Use of a Programmed Temperature Injector for On-Line Reversed-Phase Liquid Chromatography-Capillary Gas Chromatography (1995)

A simple procedure is described that allows on-line reversed-phase liquid chromatography-gas chromatography (LC-GC); the procedure involves solvent split elimination using a programmed temperature vaporizer (PTV) packed with a suitable trapping material. Eight experimental variables affecting the PTV transfer of the LC fractions into the GC system are evaluated, and conditions adequate for the analysis of various sterols are established. The proposed procedure does not require a phase switching interface (i.e., an on-line transfer of the analytes from the aqueous phase to an organic phase prior to transfer), thus the experimental setup generally required for on-line reversed-phase LC-GC is simplified. The system allows direct analysis of sample volumes up to 1600 μL with injection speeds up to 1500 µL/min. Relative standard deviations less than 10.5% are obtained even though different glass liners are used for the experimental measurements.
Supplementary Figure 2 from Molecular Imaging of TGFβ-Induced Smad2/3 Phosphorylation Reveals a Role for Receptor Tyrosine Kinases in Modulating TGFβ Signaling (2023)

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TWIST1 interacts with adherens junction proteins during neural tube development and regulates fate transition in cranial neural crest cells (2021)

ABSTRACT Cell fate determination is a necessary and tightly regulated process for producing different cell types and structures during development. Cranial neural crest cells (CNCCs) are unique to vertebrate embryos and emerge from the neural fold borders into multiple cell lineages that differentiate into bone, cartilage, neurons, and glial cells. We previously reported that Irf6 genetically interacts with Twist1 during CNCC-derived tissue formation. Here, we investigated the mechanistic role of Twist1 and Irf6 at early stages of craniofacial development. Our data indicates that TWIST1 interacts with α/β/γ-CATENINS during neural tube closure, and Irf6 is involved in the structural integrity of the neural tube. Twist1 suppresses Irf6 and other epithelial genes in CNCCs during epithelial-to-mesenchymal transition (EMT) process and cell migration. Conversely, a loss of Twist1 leads to a sustained expression of epithelial and cell adhesion markers in migratory CNCCs. Disruption of TWIST1 phosphorylation in vivo leads to epidermal blebbing, edema, neural tube defects, and CNCC-derived structural abnormalities. Altogether, this study describes an uncharacterized function of Twist1 and Irf6 in the neural tube and CNCCs and provides new target genes of Twist1 involved in cytoskeletal remodeling. Furthermore, the association between DNA variations within TWIST1 putative enhancers and human facial morphology is also investigated. SUMMARY STATEMENT This study uncovers a new function of Twist1 in neural tube development and epithelial-to-mesenchymal transition in cranial neural crest cells. Data further shows that Twist1 -interacting Irf6 is involved in regulating neural tube integrity.
Microsample liquid analysis with a wire loop direct sample insertion technique in an inductively coupled plasma (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicrosample liquid analysis with a wire loop direct sample insertion technique in an inductively coupled plasmaEric D. Salin and R. L. A. SingCite this: Anal. Chem. 1984, 56, 13, 2596–2598Publication Date (Print):November 1, 1984Publication History Published online1 May 2002Published inissue 1 November 1984https://pubs.acs.org/doi/10.1021/ac00277a074https://doi.org/10.1021/ac00277a074research-articleACS PublicationsRequest reuse permissionsArticle Views29Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Right Ventricular Plasticity and Functional Imaging (2012)

Right ventricular (RV) function is a strong independent predictor of outcome in a number of distinct cardiopulmonary diseases. The RV has a remarkable ability to sustain damage and recover function which may be related to unique anatomic, physiologic, and genetic factors that differentiate it from the left ventricle. This capacity has been described in patients with RV myocardial infarction, pulmonary arterial hypertension, and chronic thromboembolic disease as well as post-lung transplant and post-left ventricular assist device implantation. Various echocardiographic and magnetic resonance imaging parameters of RV function contribute to the clinical assessment and predict outcomes in these patients; however, limitations remain with these techniques. Early diagnosis of RV function and better insight into the mechanisms of RV recovery could improve patient outcomes. Further refinement of established and emerging imaging techniques is necessary to aid subclinical diagnosis and inform treatment decisions.
Chronic exposure to leucine in vitro induces β-cell dysfunction in INS-1E cells and mouse islets (2012)

Chronic hyperglycemia and hyperlipidemia cause deleterious effects on β-cell function. Interestingly, increased circulating amino acid (AA) levels are also a characteristic of the prediabetic and diabetic state. The chronic effects of AAs on β-cell function remain to be determined. Isolated mouse islets and INS-1E cells were incubated with or without excess leucine. After 72 h, leucine increased basal insulin secretion and impaired glucose-stimulated insulin secretion in both mouse islets and INS-1E cells, corroborating the existence of aminoacidotoxicity-induced β-cell dysfunction. This took place concomitantly with alterations in proteins and genes involved in insulin granule transport, trafficking (e.g. collapsin response mediator protein 2 and GTP-binding nuclear protein Ran), insulin signal transduction (proteasome subunit α type 6), and the oxidative phosphorylation pathway (cytochrome c oxidase). Leucine downregulated insulin 1 gene expression but upregulated pancreas duodenum homeobox 1 and insulin 2 mRNA expressions. Importantly, cholesterol (CH) accumulated in INS-1E cells concomitantly with upregulation of enzymes involved in CH biosynthesis (e.g. 3-hydroxy-3-methylglutaryl-CoA reductase, mevalonate (diphospho) decarboxylase, and squalene epoxidase) and LDL receptor, whereas triglyceride content was decreased. Our findings indicate that chronic exposure to elevated levels of leucine may have detrimental effects on both β-cell function and insulin sensitivity. Aminoacidotoxicity may play a pathogenic role in the development of type 2 diabetes.
Vertical sleeve gastrectomy reduces hepatic steatosis while increasing serum bile acids in a weight-loss-independent manner (2013)

VSG in obese mice results in greater hepatic steatosis reduction than seen with caloric restriction alone. VSG surgery increases serum bile acids that correlate with weight lost postsurgery and changes serum bile composition that could explain suppression of hepatic genes responsible for lipogenesis.
HEPATOSIS DIETETICA, NUTRITIONAL MYOPATHY, MULBERRY HEART DISEASE AND ASSOCIATED HEPATIC SELENIUM LEVELS IN PIGS (1979)

Outbreaks of Hepatosis Dietetica (HD), Nutritional Myopathy (NM) and Mulberry Heart Disease (MHD) in Western Australia are described. Hepatic selenium concentrations were low in pigs with HD and NM, but not with MHD. The mean hepatic selenium concentration in pigs unaffected with these conditions was 1.07 +/- 0.12 ppm dry wt. Cereal grain in an area of Western Australia where HD and NM frequently occur was found to be low in selenium and the addition of 0.1 ppm selenium to pig rations appeared to be an effective prophylactic measure. The extensive use of lupin seed as a protein source replacing much of the meat meal in pig rations may have contributed to an increase in the incidence of HD and NM.
Proton acceleration from magnetized overdense plasmas (2017)

Proton acceleration by an ultraintense short pulse circularly polarized laser from an overdense three dimensional (3D) particle-in-cell (PIC) 3D-PIC simulations. The axial magnetic field modifies the dielectric constant of the plasma, which causes a difference in the behaviour of ponderomotive force in case of left and right circularly polarized laser pulse. When the laser is right circularly polarized, the ponderomotive force gets enhanced due to cyclotron effects generating high energetic electrons, which, on reaching the target rear side accelerates the protons via target normal sheath acceleration process. On the other hand, in case of left circular polarization, the effects get reversed causing a suppression of the ponderomotive force at a short distance and lead towards a rise in the radiation pressure, which results in the effective formation of laser piston. Thus, the axial magnetic field enhances the effect of radiation pressure in case of left circularly polarized laser resulting in the generation of high energetic protons at the target front side. The transverse motion of protons get reduced as they gyrate around the axial magnetic field which increases the beam collimation to some extent. The optimum thickness of the overdense plasma target is found to be increased in the presence of an axial magnetic field.
Gene Therapy for Restenosis (1995)

Hyperplasia of smooth muscle cells (SMC) in the artery wall is an essential component of atherosclerosis, restenosis following angioplasty and allograft rejection after heart transplantation. The inhibition of SMC migration, along with proliferation, has long been investigated as a potential strategy for the treatment of cardiovascular disease. In vivo transfer of genetic material into the arterial wall offers the devolopment of a medical intervention to alter the genetic program of SMC for therapeutic purposes. Given that local vascular diseases such as atherosclerosis and restenosis are caused by abnormalities of gene expression, it is logical to attempt to treat these diseases through the gene replacement, the antisense inactivation of gene expressiton or the augmentation of expression of genes that are capable of playing a therapeutic role. To date, however, no therapeutic genes for preventing intimal accumulation of SMC in humans have yet been reported. We summarize recent gene therapy strategies for restenosis with special reference to the biological effects of transfer of the human calponin gene, encoding an actin-binding protein which has sequence similarity with the Vav Ras/Rho nucleotide exchange factor and a yeast gene CDC24, into the arteries of live animals. Our results suggest that overexpression of calponin, through its ability to suppress SMC growth and migration may inhibit arterial myointimal hyperplasia in vivo. Direct gene transfer of calponin into the arterial wall may thus be of potential therapeutic value in cardiovascular disease.
XWRC: externally-loadedweighted random pattern testing for input test data compression (2006)

This paper presents an input test data compression scheme that combines the advantages of weighted pseudorandom testing techniques and LFSR reseeding. The scheme requires low area overhead and the compression achieved is not limited by the LFSR reseeding bounds. The test data storage requirements of both the static and dynamic versions of the proposed scheme are lower than previously published results. The total numbers of test patterns that need to be applied are much lower than that for any weighted pseudorandom testing or hybrid BIST scheme. Further, the method provides an easy way to trade off between test application time and test data compression. The static version of the scheme can be easily implemented without any modification to the current test generation flow while the dynamic version can be used if the ATPG can be modified. Experimental results on a large industry design show that over 100/spl times/ compression is achievable by the proposed scheme.
Domain Adaptive Segmentation In Volume Electron Microscopy Imaging (2019)

In the last years, automated segmentation has become a necessary tool for volume electron microscopy (EM) imaging. So far, the best performing techniques have been largely based on fully supervised encoder-decoder CNNs, requiring a substantial amount of annotated images. Domain Adaptation (DA) aims to alleviate the annotation burden by `adapting' the networks trained on existing groundtruth data (source domain) to work on a different (target) domain with as little additional annotation as possible. Most DA research is focused on the classification task, whereas volume EM segmentation remains rather unexplored. In this work, we extend recently proposed classification DA techniques to an encoder-decoder layout and propose a novel method that adds a reconstruction decoder to the classical encoder-decoder segmentation in order to align source and target encoder features. The method has been validated on the task of segmenting mitochondria in EM volumes. We have performed DA from brain EM images to HeLa cells and from isotropic FIB/SEM volumes to anisotropic TEM volumes. In all cases, the proposed method has outperformed the extended classification DA techniques and the finetuning baseline. An implementation of our work can be found on https://github.com/JorisRoels/domain-adaptive-segmentation.
MiR-339 inhibits proliferation of pulmonary artery smooth muscle cell by targeting FGF signaling (2017)

Pulmonary artery hypertension (PAH) is a fatal disorder. Recent studies suggest that microRNA (miRNA) plays an important role in regulating proliferation of pulmonary artery smooth muscle cells (PASMC), which underlies the pathology of PAH However, the exact mechanism of action of miRNAs remains elusive. In this study, we found that miR-339 was highly expressed in the cardiovascular system and was downregulated by a group of cytokines and growth factors, especially PDGF-BB and FGF2. Functional analyses revealed that miR-339 can inhibit proliferation of PASMC Also, miR-339 inhibited FGF2-induced proliferation, but had no effect on proliferation induced by PDGF-BB The fibroblast growth factor receptor substrate 2 (FRS2) was identified as a potential direct target of miR-339. Consistent with the actions of miR-339, knockdown of FRS2 only inhibited FGF2- but not PDGF-BB-induced proliferation of PASMC In addition, our results showed that inhibition of ERK and PI3K abrogated the downregulation of miR-339 induced by PDGF-BB Finally, miR-339 expression was found to be decreased in the pulmonary arteries of rats with MCT-induced PAH Our study is the first report on the biological role of miR-339 in regulating proliferation of PASMC by targeting FGF signaling, providing new mechanistic insights into PASMC proliferation and pathogenesis of PAH.
Molecular Cloning, Characterization, and Expression of a Human 14-kDa Lectin (1989)

Full length cDNAs coding for a 14-kDa beta-galactoside binding lectin have been isolated from HL-60 cells and human placenta. Oligonucleotide probes based on a pentapeptide present in several partial sequences of homologous human lectins were used to screen a lambda GT10 HL-60 cDNA library. The HL-60 cDNA clones that were isolated were used to design a synthetic primer representing the 3'-untranslated region of the HL-60 lectin. This primer was then used to synthesize a lambda GT10 human placenta cDNA library, and restriction fragments of the HL-60 cDNA clones were used to screen the library. The cDNA clones for both HL-60 and placenta lectin had identical sequences with short 5'- and 3'-untranslated regions and coded for a 135-amino acid protein which lacks a hydrophobic signal peptide sequence. Biochemical data show that, despite the presence of a possible N-linked glycosylation site, the protein is not glycosylated. Northern and Southern blot analyses indicate that the 14-kDa lectin is encoded for by a single gene. The lectin cDNA was expressed in Escherichia coli and biologically active protein was purified from cell lysates by affinity chromatography.
Inorganic Semiconductor Nanowires: Rational Growth, Assembly, and Novel Properties (2002)

Rationally controlled growth of inorganic semiconductor nanowires is important for their applications in nanoscale electronics and photonics. In this article, we discuss the rational growth, physical properties, and integration of nanowires based on the results from the authors' laboratory. The composition, diameter, growth position, and orientation of the nanowires are controlled based on the vapor-solid-liquid (VLS) crystal growth mechanism. The thermal stability and optical properties of these semiconductor nanowires are investigated. Particularly, ZnO nanowires with well-defined end surfaces can function as room-temperature ultraviolet nanolasers. In addition, a novel microfluidic-assisted nanowire integration (MANI) process was developed for the hierarchical assembly of nanowire building blocks into functional devices and systems.
On the viscous boundary layer near the center of the resistive reconnection region (1998)

This paper studies the behavior of the magnetic field near the center of the reconnection layer in the framework of two-dimensional incompressible resistive magnetohydrodynamics with uniform resistivity in a steady state. Priest and Cowley [J. Plasma Phys. 14, 271 (1975)] have presented an argument showing that when the viscosity ν is zero, the magnetic separatrices do not cross at a finite angle but osculate at the X-point. In the present paper it is shown that this conclusion is in fact not correct. First, some results of numerical simulations of the reconnection layer are presented. These results contradict the conclusions of Priest and Cowley. To explain this contradiction, an analytical theory for the neighborhood of the X-point is developed. When viscosity ν is small, a narrow boundary layer develops near the neutral point. Some of the higher derivatives of the stream function Φ become very large near the X-point, leading to a nonzero angle between the separatrices. As ν→0, the boundary layer shrinks and nonanalytic logarithmic terms emerge in the expansion of Φ in the outer region. This makes the Taylor expansion of Priest and Cowley invalid in the case ν=0. The results of our boundary layer analysis are in good agreement with the numerical simulations.
Differing effects of 2 active dried yeast (Saccharomyces cerevisiae) strains on ruminal acidosis and methane production in nonlactating dairy cows (2011)

Fifteen ruminally cannulated, nonlactating Holstein cows were used to measure the effects of 2 strains of Saccharomyces cerevisiae, fed as active dried yeasts, on ruminal pH and fermentation and enteric methane (CH(4)) emissions. Nonlactating cows were blocked by total duration (h) that their ruminal pH was below 5.8 during a 6-d pre-experimental period. Within each block, cows were randomly assigned to control (no yeast), yeast strain 1 (Levucell SC), or yeast strain 2 (a novel strain selected for enhanced in vitro fiber degradation), with both strains (Lallemand Animal Nutrition, Montréal, QC, Canada) providing 1 × 10(10) cfu/head per day. Cows were fed once daily a total mixed ration consisting of a 50:50 forage to concentrate ratio (dry matter basis). The yeast strains were dosed via the rumen cannula daily at the time of feeding. During the 35-d experiment, ruminal pH was measured continuously for 7 d (d 22 to 28) by using an indwelling system, and CH(4) gas was measured for 4 d (d 32 to 35) using the sulfur hexafluoride tracer gas technique (with halters and yokes). Rumen contents were sampled on 2 d (d 22 and 26) at 0, 3, and 6h after feeding. Dry matter intake, body weight, and apparent total-tract digestibility of nutrients were not affected by yeast feeding. Strain 2 decreased the average daily minimum (5.35 vs. 5.65 or 5.66), mean (5.98 vs. 6.24 or 6.34), and maximum ruminal pH (6.71 vs. 6.86 or 6.86), and prolonged the time that ruminal pH was below 5.8 (7.5 vs. 3.3 or 1.0 h/d) compared with the control or strain 1, respectively. The molar percentage of acetate was lower and that of propionate was greater in the ruminal fluid of cows receiving strain 2 compared with cows receiving no yeast or strain 1. Enteric CH(4) production adjusted for intake of dry matter or gross energy, however, did not differ between either yeast strain compared with the control but it tended to be reduced by 10% when strain 2 was compared with strain 1. The study shows that different strains of S. cerevisiae fed as active dried yeasts vary in their ability to modify the rumen fermentative pattern in nonlactating dairy cows. Because strain 2 tended (when compared with strain 1) to lower CH(4) emissions but increase the risk of acidosis, it may be prudent to further evaluate this strain in cattle fed high-forage diets, for which the risk of acidosis is low but CH(4) emissions are high.
Affibody molecules: new protein domains for molecular imaging and targeted tumor therapy. (2007)

Molecular imaging shows promise as a useful tool to aid drug discovery and development and also to provide important prognostic and predictive diagnostic information affecting patient management in the clinic. However, the use of molecular imaging diagnostically is not widely adopted, in part due to the lack of suitable targeting agents. Affibody molecules are a class of small and very stable protein domains, which can be used to selectively address a wide range of protein targets. Their small size enables high contrast radionuclide imaging and they can be produced by conventional peptide synthesis methods. Their potential utility in molecular imaging is highlighted in a large number of animal studies using anti-HER2 Affibody tracers and has recently been validated in breast cancer patients with HER2-expressing metastases. The therapeutic efficacy of the Affibody molecules in this indication was demonstrated in preclinical models using a targeted radionuclide as the effector function. This review will focus on the recent use of Affibody molecules for molecular imaging and their application for radioimmunotherapy.
Electrochemical Synthesis and Fast Electrochromics of Poly(3,4-ethylenedioxythiophene) Nanotubes in Flexible Substrate (2005)

We describe a new electrochemical synthetic method and characterization of poly(3,4-ethylenedioxythiophene) (PEDOT) nanotubes in a polycarbonate membrane. The very thin nanotubular structures in the highly flexible template provide fast electrochromic response (20 ms for oxidation or decoloration) even in a rolled state.
1,3,6,8-Tetrasubstituted Pyrenes: Solution-Processable Materials for Application in Organic Electronics (2010)

A series of star-shaped organic semiconductors have been synthesized from 1,3,6,8-tetrabromopyrene. The materials are soluble in common organic solvents allowing for solution processing of devices such as light-emitting diodes (OLEDs). One of the materials, 1,3,6,8-tetrakis(4-butoxyphenyl)pyrene, has been used as the active emitting layer in simple solution-processed OLEDs with deep blue emission (CIE = 0.15, 0.18) and maximum efficiencies and brightness levels of 2.56 cd/A and &gt;5000 cd/m&lt;sup&gt;2&lt;/sup&gt;, respectively.