gebhart/patrepeval huggingface dataset:
Chemical Investigation of the Genus Rheedia, V. Biflavonoids and Xanthochymol (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical Investigation of the Genus Rheedia, V. Biflavonoids and XanthochymolB. Botta, M. Marquina Mac-Quhae, G. Delle Monache, F. Delle Monache, and J. F. De MelloCite this: J. Nat. Prod. 1984, 47, 6, 1053Publication Date (Print):November 1, 1984Publication History Published online1 July 2004Published inissue 1 November 1984https://pubs.acs.org/doi/10.1021/np50036a033https://doi.org/10.1021/np50036a033research-articleACS PublicationsRequest reuse permissionsArticle Views210Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Quantification of Multicellular Organization, Junction Integrity, and Substrate Features in Collective Cell Migration (2017)

Quantitative analysis of multicellular organization, cell-cell junction integrity, and substrate properties is essential to understand the mechanisms underlying collective cell migration. However, spatially and temporally defining these properties is difficult within collectively migrating cell groups due to challenges in accurate cell segmentation within the monolayer. In this paper, we present Matlab®-based algorithms to spatially quantify multicellular organization (migration distance, interface roughness, and cell alignment, area, and morphology), cell-cell junction integrity, and substrate features in confocal microscopy images of two-dimensional collectively migrating endothelial monolayers. We used novel techniques, including measuring the migrating front roughness using a parametric curve formulation, automatically binning cells to obtain data as a function of distance from the migrating front, using iterative morphological closings to fully define cell boundaries, quantifying β-catenin localization as a measure of cell-cell junction integrity, and skeletonizing fibronectin to determine fiber length and orientation. These algorithms are widely accessible, as they use common fluorescent markers and Matlab® functions, and provide high-throughput critical feature quantification within collectively migrating cell groups. These image analysis algorithms can help standardize feature quantification among different experimental techniques, cell types, and research groups studying collective cell migration.
Miniature packaged external-cavity semiconductor laser with 50 GHz continuous electrical tuning range (1988)

Miniature, packaged, grating-external-cavity lasers have been developed for use in PSK. and DPSK coherent transmission systems at 1.5μm. The outside dimensions of the package are 30 × 30 × 50 mm and the optical cavity length is nominally 30 mm, resulting in a spectral linewidth &lt; 100 kHz. The lasing wavelength is mechanically adjustable over the range 1510–1560 nm and electrically tunable over a range of 0.8 nm. With appropriate control of the grating piezos, a continuous (hop-free) tuning range of 50 GHz (0.4 nm) can be obtained.
The Regulation of the Na/Ca Exchanger and Plasmalemmal Ca2+ ATPase by Other Proteins (2007)

Na/Ca exchanger (NCX) and plasma membrane Ca2+ ATPase are the Ca2+ efflux mechanisms known in mammalian cells. NCX is the main transporter to efflux intracellular Ca2+ in the heart. NCX protein contains nine putative transmembrane domains and a large intracellular loop joining two sets of the transmembrane domains. The intracellular loop regulates the activity of the NCX by interacting with other proteins and nonprotein factors, such as ions, PIP2. Several proteins that are associated with NCX have been identified recently. Similarly, plasmalemmal Ca2+ ATPase (PMCA) has 10 putative transmembrane domains, and the C-terminal intracellular region inhibits transporter activity. There are several proteins associated with PMCA, and the roles of the associated proteins of PMCA vary from specific localization to involving PMCA in signal transduction. Elucidation of structural and functional roles played by these associated proteins of NCX and PMCA will provide opportunities to develop drugs of potential therapeutic value.
Optical routing and sensing with nanowire assemblies (2005)

The manipulation of photons in structures smaller than the wavelength of light is central to the development of nanoscale integrated photonic systems for computing, communications, and sensing. We assemble small groups of freestanding, chemically synthesized nanoribbons and nanowires into model structures that illustrate how light is exchanged between subwavelength cavities made of three different semiconductors. The coupling strength of the optical linkages formed when nanowires are brought into contact depends both on their volume of interaction and angle of intersection. With simple coupling schemes, lasing nanowires can launch coherent pulses of light through ribbon waveguides that are up to a millimeter in length. Also, interwire coupling losses are low enough to allow light to propagate across several right-angle bends in a grid of crossed ribbons. The fraction of the guided wave traveling outside the wire/ribbon cavities is used to link nanowires through space and to separate colors within multiribbon networks. In addition, we find that nanoribbons function efficiently as waveguides in liquid media and provide a unique means for probing molecules in solution or in proximity to the waveguide surface. Our results lay the spadework for photonic devices based on assemblies of active and passive nanowire elements and presage the use of nanowire waveguides in microfluidics and biology.
Plasmon-phonon coupling in graphene (2010)

Collective excitations of coupled electron-phonon systems are calculated for both monolayer and bilayer graphenes, taking into account the nonperturbative Coulomb coupling between electronic excitations in graphene and the substrate longitudinal-optical phonon modes. We find that the plasmon-phonon coupling in monolayer graphene is strong at all densities but in bilayer graphene the coupling is significant only at high densities satisfying the resonant condition ${\ensuremath{\omega}}_{pl}\ensuremath{\approx}{\ensuremath{\omega}}_{ph}$. The difference arises from the peculiar screening properties associated with chirality of graphene. Plasmon-phonon coupling explains the measured quasilinear plasmon dispersion in the long-wavelength limit, thus resolving a puzzle in the experimental observations.
A study of the sulphur amino acids of rat tissues (1967)

1. In a study of the metabolism of l-[(35)S]methionine in vivo, the labelled sulphur compounds of rat liver and brain were separated first by ion-exchange chromatography into two fractions containing (i) free sulphur amino acids such as methionine, cystathionine, cyst(e)ine and homocyst(e)ine and (ii) glutathione. 2. Two-dimensional paper chromatography with butan-1-ol-acetic acid or propionic acid-water in the first direction and 80% acetone or acetone-ethyl methyl ketone-water in the second direction was found superior to other solvent systems for separating the sulphur amino acids. 3. At 10min. after injection of [(35)S]methionine only a small part of the (35)S was found combined in free methionine or other free sulphur amino acids. 4. Evidence was obtained of the presence of adenosyl[(35)S]methionine and adenosyl[(35)S]homocysteine in perchloric acid extracts of rat liver and brain. 5. The trans-sulphuration pathway was active in brain as well as in liver.
A dual polarized circular patch rectifying antenna at 2.45 GHz for microwave power conversion and detection (2002)

A novel circular patch rectifying antenna (rectenna) has been developed which converts microwave energy into DC power at 2.45 GHz. Dual polarization is achieved by two orthogonal microstrip feed lines. Rectification is achieved by GaAs Schottky-barrier diodes located on each feed line. A 48% conversion efficiency from microwave power to DC power was achieved. The design was based on a nonlinear circuit analysis.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Chalcogenide waveguides as a base for engineering broadband light sources in the mid-infrared (2011)

Chalcogenide glasses are important for applications in the mid-infrared (IR) spectral range because of their good mid-IR transparency and their high non-linear refractive indices. Our research is focused on creation of mid-IR supercontinuum sources. Numerical modelling of the ultra-short pulse propagation in various kinds of chalcogenide waveguides is complemented by experimental measurement of pulse spectrum broadening in some particular structures.
Low-power photo-carving of dye-volatile co-crystals: the sublime cutting edge of light-responsive materials (2021)

Laser beam machining (LBM) of ceramics, polymers, or metals is usually performed using high-power femtosecond lasers (4-20 W). Using LBM, micro-or nano-sized patterns can be machined into surfaces of these materials to alter their properties for various applications. A drawback of such high-power techniques is the possibility of considerable chemical damage to the surface of the machined materials.
IL-17-mediated Bcl-2 expression regulates survival of fibroblast-like synoviocytes in rheumatoid arthritis through STAT3 activation (2013)

Our data demonstrate that STAT3 is critical in IL-17-induced survival of FLS from RA patients. Therefore, therapeutic strategies that target the IL-17/STAT3 pathway might be strong candidates for RA treatment modalities.
Trinuclear Rhodium Complexes and Their Relevance for Asymmetric Hydrogenation (2008)

Various trinuclear rhodium complexes of the type [Rh(3)(PP)(3)(mu(3)-OH)(x)(mu(3)-OMe)(2-x)]BF(4) (where PP = Me-DuPhos, dipamp, dppp, dppe; different ligands and mu-bridging anions) are presented, which are formed upon addition of bases such as NEt(3) to solvate complexes [Rh(PP)(solvent)(2)]BF(4). They were extensively characterized by X-ray diffraction and NMR spectroscopy ((103)Rh, (31)P, (13)C, (1)H). Their in situ formation resulting from basic additives (NEt(3)) or basic prochiral olefins (without addition of another base) can cause deactivation of the asymmetric hydrogenation. This effect can be reversed by means of acidic additives.
Electrical Characteristics and Reliability of Wafer-on-Wafer (WOW) Bumpless Through-Silicon Via (2021)

Electrical characteristics and reliability of the wafer-on-wafer (WOW) bumpless through-silicon via (TSV) structure are investigated, and the new lumped circuit model is proposed to simulate the performance of the structure. Including the actual contact resistance in the model, the integrity of the high-frequency signal can be accurately simulated. For the 12-layer stacked bumpless TSV structure with plasma cleaning at the via bottom, the transmission loss of the signal up to 20 GHz can be lower than 2 dB. High eye height and low jitter at 3.2 Gbps show excellent signal integrity for high bandwidth memory (HBM) applications. In addition, a complete reliability study, including thermal cycling test (TCT), highly accelerated stress test (HAST), and electromigration test, reveals excellent electrical and mechanical properties, indicating that the structure is robust with great potential for 3-D integration.
Confidence Regions for Parameters of Errors-In-Variables Systems Using Sign-Perturbed Sums (2019)

In this paper, the problem of constructing non-asymptotic confidence regions for Errors-In-Variables (EIV) systems is considered. In EIV systems both the input and the output measurements are noisy. The proposed method is based upon extending the Sign-Perturbed Sums (SPS) method with instrumental variables in [1] to EIV systems. The SPS method is a state-of-the-art identification method which provides non-asymptotic confidence regions containing the true parameter with a user-chosen probability under mild assumptions on noise. The existence of two noise sources in EIV problems makes the prediction error sequence dependent, and consequently the standard SPS method is not applicable. By adopting a new regression model and perturbing the input instead of the prediction error a new method for constructing a guaranteed non-asymptotic confidence region is proposed in this paper. The method is demonstrated through a numerical experiment.
Companion recovery model to reduce the effects of profound catastrophic trauma for former child soldiers in Ganta, Liberia. (2008)

This article describes a companion recovery model designed to reduce the symptoms of PTSD in 130 former child soldiers in Ganta, Liberia. Male and female participants were conscripted by force between 6 and 13 years of age. The article discusses a model applied to a two-weeks group training and one-on-one companion intervention. The model teaches nine conceptual modules (overwhelming events, encapsulation, somatization, recognition, release, resilience, integration, new-self, and rebuilding) and a commencement ceremony to help reintegrate participants into their communities. Two case studies involving male and female participants highlight the application of this model. The study findings reveal that the participants showed significant (p ≤ .001) recovery from profound catastrophic trauma (PCT).
Studies on the structure of haptoglobin and the haptoglobin-haemoglobin complex by spin and fluorescence labelling. (1978)

Human haptoglobin (Hp) of the 1-1 type incorporated one spin or fluorescence marker per molecule; the markers were found in the beta chain. Formation of the complex between spin-labelled Hp and haemoglobin or antibody caused conformational changes in the Hp molecular, evidenced by increased participation in the electron paramagnetic resonance spectrum of the component bound with the slowly rotating marker. From fluorescence-labelled Hp, the beta chain was isolated and cleaved by CNBr; only in one of the obtained peptides, one out of 4 histidine residues was modified with the marker.
Tracing glacier changes in Austria from the Little Ice Age to the present using a lidar-based high-resolution glacier inventory in Austria (2015)

Abstract. Glacier inventories provide the basis for further studies on mass balance and volume change, relevant for local hydrological issues as well as for global calculation of sea level rise. In this study, a new Austrian glacier inventory has been compiled, updating data from 1969 (GI 1) and 1998 (GI 2) based on high-resolution lidar digital elevation models (DEMs) and orthophotos dating from 2004 to 2012 (GI 3). To expand the time series of digital glacier inventories in the past, the glacier outlines of the Little Ice Age maximum state (LIA) have been digitalized based on the lidar DEM and orthophotos. The resulting glacier area for GI 3 of 415.11 ± 11.18 km2 is 44% of the LIA area. The annual relative area losses are 0.3% yr−1 for the ~119-year period GI LIA to GI 1 with one period with major glacier advances in the 1920s. From GI 1 to GI 2 (29 years, one advance period of variable length in the 1980s) glacier area decreased by 0.6% yr−1 and from GI 2 to GI 3 (10 years, no advance period) by 1.2% yr−1. Regional variability of the annual relative area loss is highest in the latest period, ranging from 0.3 to 6.19% yr−1. The mean glacier size decreased from 0.69 km2 (GI 1) to 0.46 km2 (GI 3), with 47% of the glaciers being smaller than 0.1 km2 in GI 3 (22%).
Identification of a Peroxisomal Acyl-activating Enzyme Involved in the Biosynthesis of Jasmonic Acid in Arabidopsis (2006)

Jasmonic acid (JA) is a lipid-derived signal that regulates a wide variety of developmental and defense-related processes in higher plants. JA is synthesized from linolenic acid via an enzymatic pathway that initiates in the plastid and terminates in peroxisomes. The C18 JA precursor 12-oxo-phytodienoic acid (OPDA) is converted in the peroxisome to 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid (OPC-8:0), which subsequently undergoes three rounds of beta-oxidation to yield JA. Although most JA biosynthetic enzymes have been identified, several key steps in the pathway remain to be elucidated. To address this knowledge gap, we employed co-expression analysis to identify genes that are coordinately regulated with known JA biosynthetic components in Arabidopsis. Among the candidate genes uncovered by this approach was a 4-coumarate-CoA ligase-like member of the acyl-activating enzyme (AAE) gene family, which we have named OPC-8:0 CoA Ligase1 (OPCL1). In response to wounding, opcl1 null mutants exhibited reduced levels of JA and hyperaccumulation of OPC-8:0. Recombinant OPCL1 was active against both OPDA and OPC-8:0, as well as medium-to-long straight-chain fatty acids. Subcellular localization studies with green fluorescent protein-tagged OPCL1 showed that the protein is targeted to peroxisomes. These findings establish a physiological role for OPCL1 in the activation of JA biosynthetic precursors in leaf peroxisomes, and further indicate that OPC-8:0 is a physiological substrate for the activation step. The results also demonstrate the utility of co-expression analysis for identification of factors that contribute to jasmonate homeostasis.
Antibodies to Low-Copy Number RBC Alloantigen Convert a Tolerogenic Stimulus to an Immunogenic Stimulus in Mice (2021)

Red blood cells expressing alloantigens are well known to be capable of inducing robust humoral alloantibody responses both in transfusion and pregnancy. However, the majority of transfusion recipients and pregnant women never make alloantibodies, even after repeat exposure to foreign RBCs. More recently, RBCs have been used as a cellular therapeutic-very much like transfusion, engineered RBCs are highly immunogenic in some cases but not others. In animal models of both transfusion and RBC based therapeutics, RBCs that do not induce an immune response also cause tolerance. Despite a robust phenomenology, the mechanisms of what regulates immunity vs. tolerance to RBCs remains unclear. However, it has been reported that copy number of alloantigens on the RBCs is a critical factor, with a very low copy number causing non-responsiveness (in both humans and mice) and also leading to tolerance in mice. Recently, we reported that an IgG2c specific for an RBC antigen can substantially enhance the humoral immune response upon transfusion of RBCs expressing that antigen. Herein, we report that an IgG2c converts RBCs with low antigen copy number from a tolerogenic to an immunogenic stimulus. These findings report the first known stimulus that induces humoral alloimmunization to a low copy number RBC alloantigen and identify a previously undescribed molecular switch that has the ability to affect responder vs. non-responder phenotypes of transfusion recipients.
Determination of the Maximum Tolerated Dose of Cyclophosphamide in Conjunction with High-Dose Etoposide and Carboplatin Followed by Autologous CD34+ Hematopoietic Rescue in Pediatric Patients with Recurrent/Refractory Solid Tumors. (2006)

Abstract Cyclophosphamide (Cy) is an alkylating agent utilized in almost all preparative regimens for hematopoietic stem cell transplantation, and in vitro sensitivity has been demonstrated with carboplatin and etoposide. Multiple previous studies have involved this combination of agents; however, they almost exclusively utilized a fixed dose of Cy and/or bolus administration. The potential fatal cardiac toxicity of Cy is likely related to high peak concentrations of active metabolites from bolus dosing. Additionally, some evidence exists that the increased metabolism of Cy with continuous infusion has a therapeutic benefit. The objective of this study was to determine the maximum tolerated dose (MTD) of continuous infusion Cy with fixed dose etoposide (2400 mg/m2) and carboplatin (2175 mg/m2) followed by autologous CD34+ hematopoietic rescue in pediatric patients with recurrent/refractory solid tumors. Twenty patients were enrolled, and fourteen patients completed therapy on study. The MTD of Cy was 60 mg/kg/24 hr by continuous 60 hr infusion on days -4 to -2 for a total dose of 150 mg/kg. Alopecia, stomatitis and nausea were the most common toxicities. The single episode of direct cardiac toxicity was reversible. Two Grade IV toxicities occurred, consisting of diarrhea in one patient and coma in another patient who recovered and remains a long-term survivor. All patients were evaluable for neutrophil engraftment, with a median time to ANC &gt; 200/μl of 11 days (range 7–30 days). Thirteen of the fourteen patients were evaluable for platelet engraftment, with a median time to platelet count of 20,000/μl independent of transfusion of 21 days (range 11–72 days). Six out of the fourteen patients remain alive greater than five years after study completion. The MTD of Cy was lower than expected. These results suggest that autoinduction of Cy metabolism (repeated administration of Cy at 24 hour intervals leads to increased hepatic cytochrome enzymes) could be a primary factor in the determination of the MTD. Future studies should aim to further characterize the pharmacokinetics and effectiveness of continuous infusion Cy in pediatric patients.
A case report of early diagnosis of asymptomatic hairy cell leukemia using flow cytometry (2023)

Hairy Cell Leukemia is an infrequent leukemia that can be recognized both microscopically and flow cytometrically once the patient develops symptoms. We present a case where early diagnosis was achieved using flow cytometry long before the patient became symptomatic. This was achieved by focusing on a small percentage (0.9%) of total leukocytes that exhibited a higher side scatter and brighter CD19/CD20 than the remaining lymphocytes. A bone marrow aspirate three weeks later confirmed the presence of malignant B-cells. Shortly after, the patient presented splenomegaly and complained of fatigue.
Inhibition of activation-induced death in T cell hybridomas by thiol antioxidants: oxidative stress as a mediator of apoptosis (1994)

N-Acetylcysteine (NAC) is a well established thiol antioxidant which, after uptake, deacylation and conversion to glutathione functions as both a redox buffer and a reactive oxygen intermediate scavenger. We report here that NAC completely blocks activation induced death and associated DNA fragmentation of myelin basic protein (MBP) specific T cell hybridomas. Conversely, NAC had very little effect on the antigen driven proliferation of a MBP specific T cell line similar to that from which the hybridomas were derived. NAC displayed an analogous absolute inhibition of mitogen mediated activation induced death, even if added up to 3 h post activation. Although glutathione was as efficient as NAC at blocking activation induced death, dithiothreitol displayed minimal inhibition while L-cysteine had no effect at all. The observation that certain thiol antioxidants such as NAC and glutathione can completely block the activation induced death of T cell hybridomas implicates redox regulation in this process.