gebhart/patrepeval huggingface dataset:
Proliferation of CD4+ lymphocytes in a patient with chronic graft-versus-host disease after allogeneic bone marrow transplantation. (2000)

Expansion of donor-derived lymphocytes after allogeneic stem cell transplantation is a serious and sometimes fatal complication. Lymphoproliferative disorders are reportedly caused mainly by reactivation of Epstein-Barr virus (EBV) and non-EBV-associated secondary lymphoma or leukemia. In this paper, we report massive proliferation of CD4+ lymphocytes in peripheral blood of a patient with chronic graft-versus-host disease (GVHD) following allogeneic bone marrow transplantation (alloBMT) from an HLA-identical sibling donor. The abnormal lymphocytes showed CD3low, CD4+, CD8-, CD2+, CD5+, CD7+, CD25-, CD19-, CD20-, CD21-, CD16-, CD56low, T-cell receptor (TCR)-alpha/beta- and TCR-gamma/delta- phenotypes, and no rearrangement of either TCR-C beta 1 or IG(H)JH was detected from the lymphocytes by Southern blot analysis. EBV was not found in the nuclei of lymphocytes by an immunofluorescence antibody. The lymphoproliferation was resistant against immunosuppressive drugs, administered for the treatment of chronic GVHD, and it effectively inhibited aggravation of the chronic GVHD. Although antithymocyte globulin and cytosine arabinoside were administered later, the patient died of respiratory failure with bilateral pleural effusion and interstitial pneumonia. Because we found no evidence of monoclonality of the abnormal lymphocytes, we could not conclude that this patient had suffered from malignant lymphoproliferation. To our knowledge, this is the first case report of proliferation of CD4+ lymphocytes in a patient with chronic GVHD following alloBMT. In this paper, we discuss the possible pathophysiology of the patient.
Bioactive compounds isolated from submerged fermentations of the Chilean fungusStereum rameale (2015)

Liquid fermentations of the fungus Stereum rameale (N° 2511) yielded extracts with antibacterial activity. The antibacterial activity reached its peak after 216 h of stirring. Bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. Three known compounds were identified: MS-3 (1), vibralactone (2) and vibralactone B (3). The three compounds showed antibacterial activity as a function of their concentration. Minimal bactericidal concentrations (MBC) of compound 1 against Gram-positive bacteria were as follows: Bacillus cereus (50 μg/mL), Bacillus subtilis (10 μg/mL) and Staphylococcus aureus (100 μg/mL). Compounds 2 and 3 were active only against Gram-negative bacteria. The MBC of compound 2 against Escherichia coli was 200 μg/mL. Compound 3 inhibited significantly the growth of E. coli and Pseudomonas aeruginosa, with MBC values of 50 and 100 μg/mL, respectively.
Urgent Care Centers Delay Emergent Surgical Care Based on Patient Insurance Status in The United States (2020)

Only one-third of UCCs across the United States referred an apparent emergent surgical case to the ED, potentially delaying care. Medicaid patients were more likely to be referred directly to the ED versus privately insured patients. All patients triaged by clinicians were significantly more likely to be referred to the ED; however, the disparity between private and Medicaid patients remained.
Correction for Zhang et al., Small-molecule synergist of the Wnt/β-catenin signaling pathway (2007)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
High-Resolution Structure of the Histidine-Containing Phosphocarrier Protein (HPr) fromStaphylococcus aureusand Characterization of Its Interaction with the Bifunctional HPr Kinase/Phosphorylase (2004)

A high-resolution structure of the histidine-containing phosphocarrier protein (HPr) from Staphylococcus aureus was obtained by heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopy on the basis of 1,766 structural restraints. Twenty-three hydrogen bonds in HPr could be directly detected by polarization transfer from the amide nitrogen to the carbonyl carbon involved in the hydrogen bond. Differential line broadening was used to characterize the interaction of HPr with the HPr kinase/phosphorylase (HPrK/P) of Staphylococcus xylosus, which is responsible for phosphorylation-dephosphorylation of the hydroxyl group of the regulatory serine residue at position 46. The dissociation constant Kd was determined to be 0.10 +/- 0.02 mM at 303 K from the NMR data, assuming independent binding. The data are consistent with a stoichiometry of 1 HPr molecule per HPrK/P monomer in solution. Using transversal relaxation optimized spectroscopy-heteronuclear single quantum correlation, we mapped the interaction site of the two proteins in the 330-kDa complex. As expected, it covers the region around Ser46 and the small helix b following this residue. In addition, HPrK/P also binds to the second phosphorylation site of HPr at position 15. This interaction may be essential for the recognition of the phosphorylation state of His15 and the phosphorylation-dependent regulation of the kinase/phosphorylase activity. In accordance with this observation, the recently published X-ray structure of the HPr/HPrK core protein complex from Lactobacillus casei shows interactions with the two phosphorylation sites. However, the NMR data also suggest differences for the full-length protein from S. xylosus: there are no indications for an interaction with the residues preceding the regulatory Ser46 residue (Thr41 to Lys45) in the protein of S. xylosus. In contrast, it seems to interact with the C-terminal helix of HPr in solution, an interaction which is not observed for the complex of HPr with the core of HPrK/P of L. casei in crystals.
Radar plots: A novel modality for displaying disparate data on the efficacy of eluxadoline for the treatment of irritable bowel syndrome with diarrhea (2018)

Data presentation in radar plot format facilitates interpretation across multiple domains, demonstrating that eluxadoline treatment led to improvements vs placebo across 13 endpoints representing the range of symptoms experienced by patients with IBS-D.
Bacteriocin of a Group B Streptococcus: Partial Purification and Characterization (1975)

The production of bacteriocin-like inhibition by two of 135 strains of group B streptococci was demonstrated during the growth of these organisms on solid nutrient media. Although a variety of gram-positive organisms was susceptible, none of a wide range of different gram-negative strains was inhibited. The active substance produced by one of these strains was extracted from cultures grown on solid media (Todd-Hewitt agar) and was shown to be bactericidal in action. This bacteriocin (streptocin B(1)) was partially purified by ammonium sulfate precipitation followed by gel filtration and ion-exchange chromatography. Production of the bacteriocin was repressed by addition of certain fermentable carbohydrates to the basic medium and enhanced by the addition of yeast extract. The bacteriocin was shown to exist in two distinct and interconvertible physical forms: a basic unit of molecular weight 10,000 and an aggregate having a molecular weight of over 200,000. The bacteriocin was inactivated by proteolytic enzymes and, although labile in alkali, it was stable to boiling in mild acids.
Complexions of compliance (2012)

Division of Hematology Oncology Massachusetts General Hospital Boston, Massachusetts Financial disclosure/conflicts of interest: R.T.P. has served in the scientific advisory boards of Genentech Inc., AstraZeneca, Lifecore Biomedical, Biomarin Pharmaceuticals Inc., Clovis Oncology, and Seattle Genetics, and has received research funding from ImClone Systems Inc., Endocyte Inc., AstraZeneca, Eisai Inc., and Amgen Inc.
Design of a digital, ultra-broadband electro-optic switch for reconfigurable optical networks-on-chip (2009)

We present a novel design for a noise-tolerant, ultra-broadband electro-optic switch, based on a Mach-Zehnder lattice (MZL) interferometer. We analyze the switch performance through rigorous optical simulations, for devices implemented in silicon-on-insulator with carrier-injection-based phase shifters. We show that such a MZL switch can be designed to have a step-like switching response, resulting in improved tolerance to drive-voltage noise and temperature variations as compared to a single-stage Mach-Zehnder switch. Furthermore, we show that degradation in switching crosstalk and insertion loss due to free-carrier absorption can be largely overcome by a MZL switch design. Finally, MZL switches can be designed for having an ultra-wide, temperature-insensitive optical bandwidth of more than 250 nm. The proposed device shows good potential as a broadband optical switch in reconfigurable optical networks-on-chip.
Detection of Specific RNA Targets by Multimerization (2023)

Detection of specific RNA targets via amplification-mediated techniques is widely used in fundamental studies and medicine due to essential role of RNA in transfer of genetic information and development of diseases. Here, we report on an approach for detection of RNA targets based on the particular type of isothermal amplification, namely, reaction of nucleic acid multimerization. The proposed technique requires only a single DNA polymerase possessing reverse transcriptase, DNA-dependent DNA polymerase, and strand-displacement activities. Reaction conditions that lead to efficient detection of the target RNAs through multimerization mechanism were determined. The approach was verified by using genetic material of the SARS-CoV-2 coronavirus as a model viral RNA. Reaction of multimerization allowed to differentiate the SARS-CoV-2 RNA-positive samples from the SARS-CoV-2 negative samples with high reliability. The proposed technique allows detection of RNA even in the samples, which were subjected to multiple freezing-thawing cycles.
Unnaturally configured 13‐epi steroids: full 1H and 13C assignments and ring C–D conformations from 1H, 1H vicinal couplings (2001)

Abstract Six steroids with inverted stereochemistry at C‐13 (13‐ epi ) were studied. Measurement of vicinal proton–proton couplings was facilitated by a 1D TOCSY experiment, which provided efficient deconvolution of the ring D protons from other signals. A simple semi‐quantitative analysis of the ring D couplings was found to be sufficient to determine the conformation of ring D. Examples of 13α, 14β half‐chair, 17β envelope and 16β envelope conformations were found. Copyright © 2001 John Wiley &amp; Sons, Ltd.
Relations between structure and nicotine‐like activity: X‐ray crystal structure analysis of (−)−cytisine and (−)−lobeline hydrochloride and a comparison with (−)−nicotine and other nicotine‐like compounds (1989)

1. Although (-)-cytisine is a rigid structure, it occurs in the crystal in two distinct but very similar conformations in which the pyridone ring is tilted relative to the charged nitrogen atom at much the same angle as the pyridine ring is in (-)-nicotine hydrogen iodide. The carbonyl group in the pyridone ring of (-)-cytisine, however, is on the side of the ring opposite to pyridine nitrogen in (-)-nicotine. 2. The pKa of (-)-lobeline HCl at 25 degrees C is 8.6 (approx), indicating that (-)-lobeline is at least 90% in the protonated form at physiological pH (7.6). It is probably the phenyl 2-keto-ethyl part of (-)-lobeline, rather than the phenyl 2-hydroxy-ethyl part, which interacts with the receptor. 3. The combination within one molecule of a charged ('onium') nitrogen atom lying out of the plane of, and some distance (4.5-6.5 A) from, an aromatic ring is common to many compounds with nicotine-like activity (e.g. nicotine, cytisine, choline phenyl ether bromide, dimethyl-phenyl-piperazinium (DMPP) iodide, coryneine iodide and m-hydroxyphenylpropyl trimethyl ammonium iodide). In some molecules the aromatic ring can be replaced by an unsaturated group, such as carbonyl (e.g. acetylcholine) or double-bonds (e.g. anatoxin). 4. Activity at nicotinic receptors appears to involve interactions between the positively charged nitrogen atom and a negatively charged group, probably close to cysteine residues 192 and 193 in the receptor. It is suggested that rather than specific groups in the molecule also being involved, activity at nicotinic receptors depends on interactions between a flat part of the drug containing double-bonds, or systems of double bonds, and a planar area in the receptor, possibly tyrosine or phenylalanine residues.
A system of conservative regridding for ice–atmosphere coupling in a General Circulation Model (GCM) (2014)

Abstract. The method of elevation classes, in which the ice surface model is run at multiple elevations within each grid cell, has proven to be a useful way for a low-resolution atmosphere inside a general circulation model (GCM) to produce high-resolution downscaled surface mass balance fields for use in one-way studies coupling atmospheres and ice flow models. Past uses of elevation classes have failed to conserve mass and energy because the transformation used to regrid to the atmosphere was inconsistent with the transformation used to downscale to the ice model. This would cause problems for two-way coupling. A strategy that resolves this conservation issue has been designed and is presented here. The approach identifies three grids between which data must be regridded and five transformations between those grids required by a typical coupled atmosphere–ice flow model. This paper develops a theoretical framework for the problem and shows how each of these transformations may be achieved in a consistent, conservative manner. These transformations are implemented in Glint2, a library used to couple atmosphere models with ice models. Source code and documentation are available for download. Confounding real-world issues are discussed, including the use of projections for ice modeling, how to handle dynamically changing ice geometry, and modifications required for finite element ice models.
Intensive Conventional Insulin Therapy for Type II Diabetes: Metabolic effects during a 6-mo outpatient trial (1993)

Intensive CIT, when combined with CBG measurements, can be used to rapidly improve glycemic control in type II diabetes without development of unacceptable hypoglycemia. This degree of metabolic improvement, however, requires large doses of exogenous insulin to overcome peripheral insulin resistance and results in greater hyperinsulinemia with progressive weight gain.
[Year-round antibody profile of groups of horses of a herd kept in isolation after differently terminating use of an experimental viral combination vaccine]. (1991)

The commercial vaccine "Resequin F Konz." devised against viral respiratory infections of horses contains the abortigenic Equine Herpesvirus-1 (EHV-1). Therefore we had used it in our protection project of the Austrian Lipizzaners+ primarily to prevent abortions. Taking into account the recent perception that for young horses the respiratory-pathogenic EHV-4 type is essential Behringwerke Marburg added this particular virus to their market product to produce a multicomponent experimental vaccine. We examined this vaccine for its antibody induction as well as their persistence against each of its viral components. On groups of foals we did this regarding its prophylactic effect against respiratory infections. Furthermore, we investigated its immunogenicity in adult horses, hoping for a potentiating effect of EHV-4 against EHV-1, mediating enhanced protection against abortion caused by the latter virus. This experimental vaccine proved excellently tolerable, its immunogenicity against either equine herpesvirus type was considerable, was very good against both equine influenza subtypes, was low, however, against retroviruses types 1 and 3. Recommendations are made for seasonal optimal spacing of vaccinations, taking into account the prevalent dissemination phases of the viruses involved, the different age groups of horses and their respective use.
Nutrition Standards for Foods in Schools: Leading the Way Toward Healthier Youth (2007)

Food choices and eating habits are learned from many sources. The school environment plays significant role in teaching and modeling health behaviors. For some children, foods consumed at school can provide major portion of their daily nutrient intake. Foods and beverages consumed at school can come from two major sources: (1) Federally funded programs that include the National School Lunch Program (NSLP), the School Breakfast Program (SBP), and after-school snacks and (2) competitive sources that include vending machines, a la carte sales in the school cafeteria, or school stores and snack bars. Foods and beverages sold at school outside of the federally reimbursable school nutrition programs are referred to as /competitive foodsa because they compete with the traditional school lunch as nutrition source. There are important concerns about the contribution of nutrients and total calories from competitive foods to the daily diets of school-age children and adolescents. Nutrition Standards for Foods in Schools offers both reviews and recommendations about appropriate nutrition standards and guidance for the sale, content, and consumption of foods and beverages at school, with attention given to foods and beverages offered in competition with federally reimbursable meals and snacks. It is sure to be an invaluable resource to parents, federal and state government agencies, educators and schools, health care professionals, food manufacturers, industry trade groups, media, and those involved in consumer advocacy.
Energy density active noise control in an earthmoving machine cab (2005)

Conventional active noise control (ANC) systems attempt to minimize squared pressure at an error sensor (or sensors) to achieve control, and often result in a relatively small zone of control. Considering a practical application in a vehicle, this approach is acceptable provided the operator’s head remains within the control zone. In the case of an earthmoving machine, an operator’s head regularly moves about the cab, either because of the motion of the machine during operation or as the operator moves about to perform various tasks. By minimizing energy density (ED), instead of squared-pressure, a potentially larger control zone may be achieved. Active control of the sound field within an earthmoving machine cab was attempted using the ED technique. The scope was limited to controlling the engine firing frequency tonal within the cab with the machine running, but in a static condition (engine idle). Frequency response and spatial results show the method achieved nearly global control within the cab and effectively reduced the A-weighted sound-pressure level by a few decibels, despite only reducing a single low frequency tone. In this sense, active control using ED has the potential to be more suitable to earthmoving machine applications than squared-pressure techniques.
Playable Environments: Video Manipulation in Space and Time (2022)

We present Playable Environments-a new representation for interactive video generation and manipulation in space and time. With a single image at inference time, our novel framework allows the user to move objects in 3D while generating a video by providing a sequence of desired actions. The actions are learnt in an unsupervised manner. The camera can be controlled to get the desired viewpoint. Our method builds an environment state for each frame, which can be manipulated by our proposed action mod-ule and decoded back to the image space with volumetric rendering. To support diverse appearances of objects, we extend neural radiance fields with style-based modulation. Our method trains on a collection of various monocular videos requiring only the estimated camera parameters and 2D object locations. To set a challenging benchmark, we in-troduce two large scale video datasets with significant cam-era movements. As evidenced by our experiments, playable environments enable several creative applications not at-tainable by prior video synthesis works, including playable 3D video generation, stylization and manipulation <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> willi-menapace.github.io/playable-environments-website.
The B isozyme of creatine kinase is active as a fusion protein in Escherichia coli: In vivo detection by 31P NMR (1989)

A cDNA encoding the B isozyme of creatine kinase (CKB) has been expressed in Escherichia coli from a fusion with lacZ carried by lambda gt11. Western blots indicate that a stable polypeptide with the appropriate mobility for the beta-galactosidase-creatine kinase (beta-gal-CKB) fusion protein cross-reacts with both beta-gal and CKB antiserum. No significant CK activity is detected in control E. coli; however, extracts from cells containing the lambda gt11-CKB construct have a CK activity of 1.54 +/- 0.07 mumol/min per mg protein. The fusion protein appears to provide this activity because immunoprecipitation of protein with beta-gal antiserum leads to a loss of CK activity from extracts. That the enzyme is active in vivo was demonstrated by detection of a phosphocreatine (PCr) peak in the 31P NMR spectrum from E. coli grown on medium supplemented with creatine. As in mammalian brain and muscle, the PCr peak detected was sensitive to the energy status of the E. coli.
Simultaneous quantification of triterpenoic acids by high performance liquid chromatography method in the extracts of gum resin of Boswellia serrata obtained by different extraction techniques (2016)

The eight triterpenoic acids coded as BS-1 (11-keto-beta-boswellic acid), BS-2 (3-O-acetyl-11-keto-beta-boswellic acid), BS-3 (3-keto tirucallic acid), BS-4 (3-O-acetyl-alpha-tirucallic acid), BS-5 (3-O-acetyl-beta-tirucallic acid), BS-6 (alpha-boswellic acid), BS-7 (beta-boswellic acid) and BS-8 (3-O-acetyl-beta-boswellic acid) were isolated from the processed gum resin of Boswellia serrata by column chromatography. The proposed HPLC method is simple, reliable and has been very useful for the qualitative as well as quantitative analysis of boswellic acids in the gum resin of Boswellia serrata. The proposed method allows to quantify boswellic acids in appreciable amounts by HPLC-UV (DAD) method in the extracts and the available marketed formulations.Graphical abstractIsolation & separation of eight Triterpenoic acids from Boswellia serrata.
Physiological and molecular mechanisms of bacterial phytostimulation. (2017)

Given the comprehensive reviews already published summarizing the suitability of bacteria as bioinoculants in horticultural crops, for enhanced crop resistance to abiotic, or biotic stresses, this review focuses on what is known about the plant-bacteria (such as plant growth promoting rhizobacteria) recognition mechanisms, the recently discovered molecules from bacteria influencing root growth and plant development, and the genes and proteins whose expression changes in plants and bacteria during the interaction.
HLA Antigens in Two Iranian Populations: The Armenians and The Jews (1978)

The distribution of HLA phenotype, genotype and haplotype frequencies was studied in two Iranian populations, the Armenians and the Jews. Although the frequencies of most antigens in the Armenians have approximately the average Jewish values, haplotype frequencies are quite different between the two populations. The frequencies of antigens in Iranian Jews are more similar to those in the Mazandaranies, a group of non‐Jewish Iranians, than to the Jewish populations from Yemen and Cochin, India.