gebhart/patrepeval huggingface dataset:
Eukaryotic topoisomerase II cleavage of parallel stranded DNA tetraplexes (1992)

A guanine-rich single-stranded DNA from the human immunoglobulin switch region was shown by Sen and Gilbert [Nature, (1988) 334, 364-366] to be able to self-associate to form a stable four-stranded parallel DNA structure. Topoisomerase II did not cleave the single-stranded DNA molecule. Surprisingly, the enzyme did cleave the same DNA sequence when it was annealed into the four-stranded structure. The two cleavage sites observed were the same as those found when this DNA molecule was paired with a complementary molecule to create a normal B-DNA duplex. These cleavages were shown to be protein-linked and reversible by the addition of salt, suggesting a normal topoisomerase II reaction mechanism. In addition, an eight-stranded DNA molecule created by the association of a complementary oligonucleotide with the four-stranded structure was also cleaved by topoisomerase II despite being resistant to restriction endonuclease digestion. These results suggest that a single strand of DNA may possess the sequence information to direct topoisomerase II to a binding site, but the site must be base paired in a proper manner to do so. This demonstration of the ability of a four-stranded DNA molecule to be a substrate for an enzyme further suggests that these DNA structures may be present in cells.
107 Mechanical Properties of Post-annealed DLC Films (2000)

The mechanical properties of the post-annealed diamond-like carbon (DLC) films were studied using nano-indentation method and ball-on-disk test. DC ion plating deposition technique has been used to deposit DLC on Si(1OO) substrates. The films were also characterized by Raman spectroscopy and thermal desorptioh spectroscopy (TDS). The hardness of the DLC films was found to decrease above 400℃. The results of Raman spectroscopy and TDS showed that at higher annealing temperatures (400℃ <) graphitization occurred for amorphous DLC with hydrogen desorption. It was found that both friction coefficient and wear rate of these films were unaffected by the post-annealing.
Association study of functional polymorphisms of dopaminergic pathway in epilepsy‐related factors of temporal lobe epilepsy in Brazilian population (2018)

Our findings demonstrated that polymorphisms of the dopaminergic pathway are associated with significant clinical features of this form of epilepsy, such as seizure frequency, family history of epilepsy and status epilepticus.
Case report: Long-term outcome of class II division 1 malocclusion treated with rapid palatal expansion and cervical traction. (2000)

A case of a Class II Division 1 malocclusion with reduced transpalatal width and unfavorable axial inclinations of the posterior teeth is reported. Rapid palatal expansion (RPE) was used for maxillary enhancement and molar distalization therapy to correct the anteroposterior dental discrepancy. This case report illustrates the results of the method of treatment used with a long-term (16-year-posttreatment) follow-up.
High speed adaptive liquid microlens array (2012)

Liquid microlenses are attractive for adaptive optics because they offer the potential for both high speed actuation and parallelization into large arrays. Yet, in conventional designs, resonances of the liquid and the complexity of driving mechanisms and/or the device architecture have hampered a successful integration of both aspects. Here we present an array of up to 100 microlenses with synchronous modulation of the focal length at frequencies beyond 1 kHz using electrowetting. Our novel concept combines pinned contact lines at the edge of each microlens with an electrowetting controlled regulation of the pressure that actuates all microlenses in parallel. This design enables the development of various shapes of microlenses. The design presented here has potential applications in rapid parallel optical switches, artificial compound eye and three dimensional imaging.
Optimized implementation of real-time image processing algorithms on field programmable gate arrays (2002)

We present the algorithm architecture "adequation" methodology for the optimized implementation of real-time image processing algorithms on field programmable gate arrays. This methodology is based on a single factorized graphs model, used from the algorithm specification down to the architecture implementation, through optimizations expressed in terms of defactorization transformations. A simple image processing example is presented to illustrate the methodology.
A novel hyperbranched polyester acrylate used for microfabrications (2004)

Abstract A novel hyperbranched polyester acrylate (HPEA) was synthesized based upon ethylenediamine tetraacetic acid as a “core” molecule, 5‐hydroxyisophthalic acid as an AB 2 monomer, and 2‐hydroxyethyl acrylate as an endcapping reagent. The obtained oligomer has an unsaturation concentration of 4.10 mmol CC g −1 measured by nuclear magnetic resonance and a wide molecular weight distribution of 1.64 measured by gel permeation chromatography. The two‐photon absorption (TPA) photopolymerization of HPEA under the exposure of a Ti : sapphire femtosecond laser with a wavelength of 800 nm was investigated through laser exposure dose‐dependant spatial resolutions of its resins. The TPA photopolymerization thresholds at the range 1.6–4.3 × 10 7 mJ cm −2 , and exposure dose windows at the range 3.4–4.3 for three formulations were determined. A spatial resolution of 0.85 μm was obtained through the TPA photopolymerization of the formulation containing 1 wt% photoinitiator and 0.3 wt% photoinhibitor. A diffraction grating and real three‐dimensional coupled gear wheel created by TPA photopolymerization were described to demonstrate the unique capability of HPEA in microfabrications. Copyright © 2004 John Wiley &amp; Sons, Ltd.
Translation term weighting and combining translation resources in cross-language retrieval (2001)

In TREC-10 the Berkeley group participated only in the English-Arabic cross-language retrieval (CLIR) track. One Arabic monolingual run and four English-Arabic cross-language runs were submitted. Our approach to the cross-language retrieval was to translate the English topics into Arabic using online EnglishArabic bilingual dictionaries and machine translation software. The five official runs are named as BKYAAA1, BKYEAA1, BKYEAA2, BKYEAA3, and BKYEAA4. The BKYAAA1 is the Arabic monolingual run, and the rest are English-to-Arabic cross-language runs. The same logistic regression based document ranking algorithm without pseudo relevance feedback was applied in all five runs. We refer the readers to the paper in [1] for details.
Synthesis of Phenols from Diazonium Tetrafluoroborates; a Useful Modification (1973)

It is shown that phenols are obtained in good yield by the decomposition of aromatic diazonium tetrafluoroborates in refluxing trifluoroacetic acid containing potassium trifluoroacetate.
Spatial Phase and Amplitude Structuring of Beams Using a Combination of Multiple Orthogonal Spatial Functions with Complex Coefficients (2016)

Analogous to time signals that can be composed of multiple frequency functions, we use uniquely structured orthogonal spatial modes to create different beam shapes. We tailor the spatial structure by judiciously choosing a weighted combination of multiple modal states within an orthogonal basis set, and we can tunably create beam phase and intensity "shapes" that are not otherwise readily achievable. As an example shape, we use a series of orbital-angular-momentum (OAM) functions with adjustable complex weights to create a reconfigurable spatial region of higher localized power as compared to traditional beam combining. We simulate a structured beam created by coherently combining several orthogonal OAM beams with different complex weights, and we achieve a &gt;10X localized power density enhancement with 19 beams. Additionally, we can create unique shapes by passing a single beam through a specially designed phase and intensity mask that contains the combination of multiple OAM functions each with complex weights. Using this approach, we experimentally demonstrate a ~2.5X localized power density increase when utilizing 9 functions.
Quadratic Stark Effect in theP322States of the Alkali Atoms (1971)

The method of pure electric field level-crossing spectroscopy has been used to measure the tensor polarizability of the $5^{2}P_{\frac{3}{2}}$ state of potassium. Our result is ${\ensuremath{\alpha}}_{2}=\ensuremath{-}0.263(40)$ MHz/ ${(\mathrm{k}\mathrm{V}/\mathrm{c}\mathrm{m})}^{2}$. Numerical values of the scalar and tensor polarizabilities of the first, second, and third $^{2}P_{\frac{3}{2}}$ states of Li, Na, K, Rb, and Cs have been calculated using the Bates-Damgaard technique to evaluate the necessary radial integrals. The polarizabilities show remarkable systematic trends among the various states of the atoms. Our present and previously reported results are compared with previous theoretical and experimental work. The agreement between theoretical and experimental values ranges from fair to excellent.
Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways (2003)

The current interest in expression of groups of functionally related genes creates a demand for novel experimental tools. We describe a multiplex ligation-dependent amplification procedure (RT-MLPA), which accurately quantifies up to 45 transcripts of interest in a one-tube assay. The output, a set of fluorescent DNA fragments, is analysed via capillary sequencer and spreadsheet software. The procedure is highly sensitive and reproducible over a 100-fold range of input RNA, with excellent compatibility with RT-PCR and microarrays. We targeted two comprehensive sets of human genes: 35 apoptosis regulators and 30 genes involved in inflammation. Both probe sets accurately assessed specific changes in gene expression in two relevant model systems. Stimulation of lymphocytes with various Toll-like receptor (TLR) ligands induced distinct inflammatory profiles. Furthermore, osteosarcoma cells treated with cytostatic drugs showed as primary response strong up-regulation of the apoptogenic p53-inducible PUMA transcript. Suppression by RNAi validated that indeed Puma expression was responsible for apoptosis induction. Thus, RT-MLPA enables relevant changes in transcription patterns to be quickly pinpointed and guide further experiments. This can be an advantage compared to hypothesis-free whole genome screens where large numbers of differentially expressed genes can obscure functional interpretation.
Un schéma OWL pour la description d'éléments de formation (2019)

International audience
Photodissociation of high molecular weight peptides and proteins in a two-stage linear time-of-flight mass spectrometer (1995)

A two-stage linear time-of-flight mass spectrometer is used to investigate the requirements for performance of laser photodissociation of peptide and protein ions. Results are presented that demonstrate that desorption and dissociation laser pulses can be synchronized to irradiate ions that travel at high velocities down the drift tube of a time-of-flight mass spectrometer. For example, 193-nm photodissociation of bovine insulin and doubly charged lysozyme is demonstrated, and laser power studies suggest that dissociation is initiated by the absorption of a single 193-nm photon. These results are encouraging because they suggest that laser photodissociation of high molecular weight proteins can lead to fragmentation on time scales compatible with time-of-flight mass spectrometry.
Chemistry of some sterically hindered mono- and diketones (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of some sterically hindered mono- and diketonesAe. De Groot, J. A. Boerma, J. De Valk, and Hans WynbergCite this: J. Org. Chem. 1968, 33, 11, 4025–4029Publication Date (Print):November 1, 1968Publication History Published online1 May 2002Published inissue 1 November 1968https://pubs.acs.org/doi/10.1021/jo01275a006https://doi.org/10.1021/jo01275a006research-articleACS PublicationsRequest reuse permissionsArticle Views72Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Detectability of Pore Defect in Wind Turbine Blade Composites Using Image Correlation Technique (2013)

풍력 발전 블레이드의 제조 및 운영 중에 발생하는 결함들은 블레이드의 수명과 안전성에 큰 영향을 미친다. 일반적으로 블레이드의 제조 과정에서는 박리, 기공, 주름, 모재 균열 등과 같은 결함이 발생한다. 본 연구에서는 이미지 상관 기법을 이용하여 변형률 분포를 확인함으로써 블레이드의 제조 과정에서 주로 나타나는 결함 중 하나인 기공 결함의 검출능을 조사하였다. 0°/±45°의 섬유 방향을 가진 4 Ply 로 적층된 GFRP 복합재 시험편에 인공적인 기공 결함을 삽입하여 기공의 크기 및 위치에 따른 검출 의존성을 조사하였다. 기공의 크기는 지름 1, 2, 3 ㎜ 이며, 기공의 위치는 시험편 표면으로부터 0.5, 1.0, 1.5 ㎜ 깊이에 삽입하였다. 부하된 시험 하중은 최대 200 ㎫ 이며, 이미지 상관 기법을 통해 변형률 분포를 획득하여 지름 2, 3 ㎜ 의 기공과 깊이 0.5, 1.0 ㎜ 의 기공 결함을 검출할 수 있었다.
SYNTHESIS AND CHARACTERIZATION OF CONTROLLED SIZES: POLYSTYRENE LATEXES (1996)

ABSTRACT Several parameters and their limitations to prepare monodisperse polystyrene particles were discussed. Polystyrene latexes B, C and D of diameter 129.8, 142.8 and 264.5 nm, respectively, were synthesized by emulsion polymerization method. The latex D is almost monodisperse with very low polydispersity of 0.005 while B and C are having polydispersity of 0.1837 and 0.1601. The particle size and particle size distribution of the latexes were determined by a TEM and Brookhaven particle size analyzer. It was observed that the particle size of the latexes decreased with increasing initiator (ammonium persulfate) or surfactant (SDS) concentrations. The surface area of the latexes were calculated by using BET equation on the basis of the amount of nitrogen gas adsorbed at the surface of the particles to form a monolayer. It was observed that the surface area of latex D is less than B and C because of the bigger size of the particles. The average molecular weights, zeta potentials and densities of the latexes were also presented.
Polyadenylate Polymerase (PAP) and 3' End pre-mRNA Processing: Function, Assays, and Association with Disease (2002)

Polyadenylate polymerase (PAP) is one of the enzymes involved in the formation of the polyadenylate tail of the 3' end of mRNA. Poly (A) tail formation is a significant component of 3' processing, a link in the chain of events, including transcription, splicing, and cleavage/polyadenylation of pre-mRNA. Transcription, capping, splicing, polyadenylation, and transport take place as coupled processes that can regulate one another. The poly(A) tail is found in almost all eukaryotic mRNA and is important in enhancing translation initiation and determining mRNA stability. Control of poly(A) tail synthesis could possibly be a key regulatory step in gene expression. PAP-specific activity values are measured by a highly sensitive assays and immunocytochemical methods. High levels of PAP activity are associated with rapidly proliferating cells, it also prevents apoptosis. Changes of PAP activity may cause a decrease in the rate of polyadenylation in the brain during epileptic seizures. Testis-specific PAP may play an important role in spermiogenesis. PAP was found to be an unfavorable prognostic factor in leukemia and breast cancer. Furthermore, measurements of PAP activity may contribute to the definition of the biological profile of tumor cells. It is crucial to know the specific target causing the elevation of serum PAP, for it to be used as a marker for disease. This review summarizes the recently accumulated knowledge on PAP including its function, assays, and association with various human diseases, and proposes future avenues for research.
Supervision in Clinical Practice (2007)

Context: Although psychotherapy supervision is accepted as acontinuing education activity by the RANZCP, and is recommendedin many Practice Guidelines, few mental health professionals-including psychiatrists-engage in such an activity.Objectives: To explain and clarify the role of psychotherapysupervision in enhancing continuing education and practice expertise.To further the appreciation of both the presence of and theeffects of psychodynamics in all interactions with patients, teamsand other mental health care providers. To encourage the acquisitionof further knowledge of psychodynamics and psychotherapiesby practitioners.Key messages: The role of supervision in clinical practice will behighlighted, as will overcoming barriers to obtaining supervision.As well, the different modalities of psychotherapy supervision aredescribed. Sexual orientation, race, and body peculiarities amongstother issues are often overlooked or unrecognized as affectingtreatment; supervision can elucidate these factors and thus improvetreatment. Individual, group or peer group supervision can bebeneficial, and experiences of each type of supervision and how toobtain them will be discussed. Becoming a supervisor is oftenresisted by individual psychiatrists, and discussion of the experiencesof supervisors both novice and long-term will clarify, andhopefully, affirm the process.Conclusion: Psychotherapy supervision is an invaluable aspect ofclinical practice and continuing education. It enhances knowledgeand treatment skills beyond what is learned in training, and thusalso assists in the avoidance of b.
Coupling of waveguide with resonator through diaphragm with slot with dielectric slab (2017)

The problem of coupling of a semi-infinite rectangular waveguide with a resonator through the narrow slot in their common end wall is solved. The slot is completely or partially filled with a dissipative dielectric. The changes in the behavior of the reflection and absorption coefficients of the feeding power are studied as a function of the dielectric constant and the thickness of the diaphragm.
Outcomes of Transcatheter Aortic Valve Replacement in Patients With Bicuspid Aortic Valve Disease (2020)

Using current-generation devices, procedural, postprocedural, and 1-year outcomes were comparable following TAVR for bicuspid AV versus tricuspid AV disease. With newer-generation devices, TAVR is a viable treatment option for patients with bicuspid AV disease.
Bio-Inspired Robust Membranes Nanoengineered from Interpenetrating Polymer Networks of Polybenzimidazole/Polydopamine (2019)

Marine mussel inspired polydopamine (PDA) has received increased attention due to its good thermal and chemical stability as well as strong adhesion on most materials. In this work, high-performance nanofiltration membranes based on interpenetrating polymer networks (IPN) incorporating PDA and polybenzimidazole (PBI) were developed for organic solvent nanofiltration (OSN). Generally, in order to obtain solvent stability, polymers need to be covalently cross-linked under harsh conditions, which inevitably leads to losses in permeability and mechanical flexibility. Surprisingly, by in situ polymerization of dopamine within a PBI support, excellent solvent resistance and permeance of polar aprotic solvents were obtained without covalent cross-linking of the PBI backbone due to the formation of an IPN. The molecular weight cutoff and permeance of the membranes can be fine-tuned by changing the polymerization time. Robust membrane performance was achieved in conventional and emerging green polar aprotic solvents (PAS) in a wide temperature range covering -10 °C to +100 °C. It was successfully demonstrated that the in situ polymerization of PDA-creating an IPN-can provide a simple and green alternative to covalent cross-linking of membranes. To elucidate the nature of the solvent stability, a detailed analysis was performed that revealed that physical entanglement along with strong secondary interaction synergistically enable solvent resistance with as low as 1-3% PDA content.
Analysis of software safety and reliability methods in cyber physical systems (2017)

The software-based systems, alone do not cause any risk; but the risk is posed when the software-based systems are considered in the context of general systems where potential risks or hazards exist. Cyber-physical systems are cited as instances of software-based systems. Nowadays, safety and reliability of cyber-physical systems are considerably important due to the increasing complexity of these systems. Risk management techniques are required to reduce the risk to an acceptable level. Generally, safety and reliability methods are important in a risk management process among them software fault tree analysis (SFTA) and software failure modes and effects analysis (SFMEA) methods can be utilised. The main purpose of this article is to provide a comprehensive survey and evaluation of the currently available approaches for software safety and reliability methods in cyber-physical systems in order to reflect the state of the art of this active area.
Interval Analysis for Guaranteed Nonlinear Parameter Estimation (1998)

Estimating the parameters of nonlinear models from experimental data often involves optimizing nonconvex cost functions. This introductory paper illustrates how interval analysis can be used to perform this task in a guaranteed way, in contrast with the usual local iterative methods.
Small-molecule screen identifies reactive oxygen species as key regulators of neutrophil chemotaxis (2010)

Neutrophil chemotaxis plays an essential role in innate immunity, but the underlying cellular mechanism is still not fully characterized. Here, using a small-molecule functional screening, we identified NADPH oxidase-dependent reactive oxygen species as key regulators of neutrophil chemotactic migration. Neutrophils with pharmacologically inhibited oxidase, or isolated from chronic granulomatous disease (CGD) patients and mice, formed more frequent multiple pseudopodia and lost their directionality as they migrated up a chemoattractant concentration gradient. Knocking down NADPH oxidase in differentiated neutrophil-like HL60 cells also led to defective chemotaxis. Consistent with the in vitro results, adoptively transferred CGD murine neutrophils showed impaired in vivo recruitment to sites of inflammation. Together, these results present a physiological role for reactive oxygen species in regulating neutrophil functions and shed light on the pathogenesis of CGD.
Birth Defects (2001)

Abstract According to the March of Dimes, the parents of one out of every 28 babies will receive the frightening news that their child has a birth defect. Web sites offer parents and caregivers of children with birth defects reliable information as well as a sense of community. In fact, some of the best Web resources are created and maintained by parents of children with birth defects. These sites are fostering a connection among families who, either because they live in remote areas or because they are coping with such rare diseases, would otherwise feel isolated. In addition, there are a number of impressive sites produced by nationally recognized charities and organizations, professional associations and societies, government agencies, research institutes, and universities. This article examines sites that are focused on birth defects in general, rather than sites devoted to specific conditions or disabilities.