gebhart/patrepeval huggingface dataset:
Differential Transcription Attenuation of Rabies Virus Genes by Intergenic Regions: Generation of Recombinant Viruses Overexpressing the Polymerase Gene (2000)

Gene expression of nonsegmented negative-sense RNA viruses involves sequential synthesis of monocistronic mRNAs and transcriptional attenuation at gene borders resulting in a transcript gradient. To address the role of the heterogeneous rabies virus (RV) intergenic regions (IGRs) in transcription attenuation, we constructed bicistronic model RNAs in which two reporter genes are separated by the RV N/P gene border. Replacement of the 2-nucleotide (nt) N/P IGR with the 5-nt IGRs from the P/M or M/G border resulted in attenuation of downstream gene transcription to 78 or 81%, respectively. A severe attenuation to 11% was observed for the 24-nt G/L border. This indicated that attenuation in RV is correlated with the length of the IGR, and, in particular, severe downregulation of the L (polymerase) gene by the 24 nt IGR. By reverse genetics, we recovered viable RVs in which the strongly attenuating G/L gene border of wild-type (wt) RV (SAD L16) was replaced with N/P-derived gene borders (SAD T and SAD T2). In these viruses, transcription of L mRNA was enhanced by factors of 1.8 and 5.1, respectively, resulting in exaggerated general gene expression, faster growth, higher virus titers, and induction of cytopathic effects in cell culture. The major role of the IGR in attenuation was further confirmed by reintroduction of the wt 24-nt IGR into SAD T, resulting in a ninefold drop of L mRNA. The ability to modulate RV gene expression by altering transcriptional attenuation is an advantage in the study of virus protein functions and in the development of gene delivery vectors.
Compression behavior of selected laser melted Al/quasicrystal composite lattice structure (2019)

In this work, Al/quasicrystal (QC) composite lattice samples with several cellular sizes ranging from 1.5 to 5 mm were manufactured using selective laser melting (SLM). The porosity of all the designed lattice structure samples is fixed as 50%. The morphology of QC reinforcement on the top surface changes from micro desquamated to nano in situ reacted, as the cellular size increases. The result indicates that cellular size has a significant influence on the compressive behavior of SLM processed lattice samples. As the cellular size increases from 1.5 to 5 mm, the fractural mechanism changes from three stages of linear elastic-plateau-densification to two stages of elastic collapse. Moreover, both the first peak compressive strength and the strain at failure decrease, when the cellular size increases. Furthermore, the SLM processed lattice 3 sample, which possesses a large cellular size, broke into small fragments after the compression test.
Table S3 from Proteomic Profiling of Extracellular Matrix Components from Patient Metastases Identifies Consistently Elevated Proteins for Developing Nanobodies That Target Primary Tumors and Metastases (2023)

&lt;p&gt;Supplementary Table S3&lt;/p&gt;
Distribution of HIV-1 Subtypes Among HIV-Seropositive Patients in the Interior of Côte d'Ivoire (2000)

Araújo, F.*; Miranda, M.†; Koch, C.*; Marques, R.†; Cunha-Ribeiro, L. M.* Author Information
Temporal phase evolution OCT for measurement of tissue deformation in the human retina in-vivo (2021)

We demonstrate the use of temporal phase evolution (TPE-) OCT methods to evaluate retinal tissue deformation <i>in-vivo</i> over time periods of several seconds. A custom built spectral domain (SD)-OCT system with an integrated retinal tracker, ensuring stable imaging with sub-speckle precision, was used for imaging. TPE-OCT measures and images phase differences between an initial reference B-scan and each of the subsequent B-scans of the evaluated temporal sequence. In order to demonstrate the precision and repeatability of the measurements, retinal nerve fiber (RNF) tissue deformations induced by retinal vessels pulsating with the heartbeat were analyzed in several healthy subjects. We show TPE maps (M-scans of phase evolution as a function of position along B-scan trace vs. time) of wrapped phase data and corresponding deformation maps in selected regions of the RNF layer (RNFL) over the course of several cardiac cycles. A reproducible phase pattern is seen at each heartbeat cycle for all imaged volunteers. RNF tissue deformations near arteries and veins up to ∼ 1.6 µm were obtained with an average precision for a single pixel of about 30 nm. Differences of motion induced by arteries and veins are also investigated.
Note. Behaviour of packaging materials during high pressure thawing Nota. Comportamiento del material para el envase durante la descongelación con altas presiones (1998)

This paper focuses on the resistance of packaging materials to piercing during high pressure thawing and delamination due to residual air. Six different packaging materials were tested, the influence of temperature, different depressurization rates and freezing were studied. Among the packaging ma terials tested, only one, BB4L®, containing EVA copolymer, was found to be resistant to piercing.
Hereditary prostate cancer in African-American families. (1998)

The recognition that family history is an important risk factor for the development of prostate cancer has led to the search for prostate cancer susceptibility genes. Although most prostate cancer linkage studies have focused on Caucasian families, there is epidemiological evidence indicating that the relative risk attributable to a positive family history is similar in African-American and Caucasian-American families. There are data to support the existence of two potential prostate cancer susceptibility genes both on the long arm of chromosome 1; these have been called HPC1 and HPC2. Studies from us and others have shown that HPC1 may contribute to the clustering of prostate cancer in some African-American families. African-American families with multiple cases of prostate cancer should be encouraged to participate in genetic research studies with the goal of identifying the molecular basis for inherited prostate cancer susceptibility in this population.
Salbutamol and inhibition of uterine contractions (1973)

Journal Article Salbutamol and inhibition of uterine contractions Get access M Hollingsworth, M Hollingsworth Department of Pharmacology, Materia Medica and Therapeutics, Medical School, Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK Search for other works by this author on: Oxford Academic Google Scholar H Schnieden H Schnieden Department of Pharmacology, Materia Medica and Therapeutics, Medical School, Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK Search for other works by this author on: Oxford Academic Google Scholar Journal of Pharmacy and Pharmacology, Volume 25, Issue 12, December 1973, Pages 996–998, https://doi.org/10.1111/j.2042-7158.1973.tb09992.x Published: 12 April 2011 Article history Received: 20 June 1973 Published: 12 April 2011
Application and research progress of lipoxygenase (2013)

Lipoxygenase widely exists in plants and microorganisms.As a potential substitutes of flour conditioner such as potassium bromate and benzoyl peroxide,it has been the research focus of green food additives.This paper reviewed the discovery history,structure,properties,application,extraction methods of lipoxygenase and its effects on the preservation of fruits and vegetables and research status.
Abstract 13502: Impact of Udenafil on Echocardiographic Indices of Single Ventricle Size and Function in Fuel Study Participants (2020)

Introduction: The Pediatric Heart Network’s Fontan Udenafil Exercise Longitudinal (FUEL) Trial (Mezzion Pharma Co. Ltd., NCT 02741115) demonstrated improvements in exercise capacity following 6 months of treatment with udenafil (87.5 mg po BID). The effect of udenafil on echocardiographic measures of single ventricle (SV) function in this cohort has not been studied. Methods: Protocol echocardiograms were obtained at baseline and 26 weeks after initiation of udenafil/placebo. Linear regression compared change from baseline in indices of SV systolic, diastolic and global function, atrioventricular valve (AVV) regurgitation and mean Fontan fenestration gradient in the udenafil cohort vs placebo, controlling for ventricular morphology (LV vs. RV/other). Effects of ventricular morphology on echo measures and its interaction over time was also evaluated. Difficult imaging windows limited consistent capture of all measures. Results: The 191 udenafil participants had significantly improved myocardial performance index (p=0.03), AVV inflow peak E and A velocities (p = 0.007 and 0.03), and annular DTI-derived peak e’ velocity (p = 0.008) compared to 195 placebo participants (Table). There were no significant differences in change in SV size, systolic function, AVV regurgitation severity or mean fenestration gradient. Although LV morphology participants had significantly more favorable indices of SV size and function (lower volumes and areas, E/e’ ratio, systolic:diastolic time and AVV regurgitation, and higher annular s’ and e’ velocity) at baseline, there was no differential effect of udenafil by ventricular morphology at 26 weeks. Conclusions: FUEL participants who received udenafil demonstrated a significant improvement in global and diastolic echo indices. The changes in diastolic function suggest improvement in pulmonary venous return and/or augmented ventricular relaxation, which may help explain improved exercise performance in that cohort.
Fabrication of albumin-micropatterned surfaces by colloidal microcontact printing technique (2013)

We report a versatile method for selective patterning of solid surfaces with albumin by a combination of microcontact printing of polymer colloids and surface charge aided protein adsorption. The technique allows formation of biocomposite arrays including colloid particles with different albumin functionalities. Micropatterns of functionalised monodisperse colloid particles were formed on the solid surfaces by using a microcontact printing method. We used polyelectrolyte multilayers (PEMs) as a coating system for poly (methylmethacrylate) acrylic acid (PMMA-AAc) patterning on glass substrate and albumin deposition onto colloid patterns by electrostatic interactions. We demonstrate that the colloid micropattern greatly improves the fluorescence imaging contrast for the deposited albumin due to its larger surface area and its immobilisation compared with patterning of albumin on a plane glass surface. This technique can be used to enhance the fluorescence imaging of biomarkers and other assays.
Valuing Bioactive Lipids from Green, Red and Brown Macroalgae from Aquaculture, to Foster Functionality and Biotechnological Applications (2020)

Marine edible macroalgae have functional proprieties that might improve human health and wellbeing. Lipids represent a minor fraction of macroalgae, yet with major interest as main carriers of omega 3 polyunsaturated fatty acids and intrinsic bioactive properties. In this study, we used lipid extracts from the green macroalgae <i>Ulva rigida</i> and <i>Codium tomentosum</i>; the red <i>Gracilaria gracilis,</i><i>Palmaria palmata</i> and <i>Porphyra dioica;</i> and the brown <i>Fucus vesiculosus</i>, produced in a land-based integrated multitrophic aquaculture (IMTA) system. We determined the lipid quality indices based on their fatty acid profiles and their bioactivities as putative antioxidant, anti-inflammatory and antiproliferative agents. The results reveal to be species-specific, namely <i>U. rigida</i> displayed the lowest atherogenicity and thrombogenicity indices. <i>Palmaria palmata</i> and <i>F. vesiculosus</i> lipid extracts displayed the lowest inhibitory concentration in the free radical scavenging antioxidant assays. <i>Ulva rigida, C. tomentosum, P. palmata</i> and <i>P. dioica</i> inhibited COX-2 activity by up to 80%, while <i>P. dioica</i> and <i>P. palmata</i> extracts showed the highest cytotoxic potential in the MDA-MB-231 breast cancer cells. This work enhances the valorization of macroalgae as functional foods and promising ingredients for sustainable and healthy diets and fosters new applications of high-valued algal biomass, in a species-specific context.
MetaMorphMagi: From Offline to Online Software Upgrades in Large-Scale IT Infrastructures (2018)

Software upgrades are one of the leading causes of downtime in IT infrastructures. Long running data migration processes require intensive up-front preparation, extended maintenance windows and close monitoring, and they impose a significant burden on the system administrators. Even worse, major upgrades sometimes fail due to complex, hidden dependencies within the system, causing unplanned downtime and loss of critical data. In this paper, we propose a technique for converting an offline data-migration process into a dependency-agnostic online upgrade that requires minimal administrative intervention and that eliminates the need for planned downtime. We illustrate our technique by walking the reader through a hypothetical, but realistic online upgrade scenario in a medium-sized IT infrastructure – namely, hot-swapping the wiki software that underlies Wikipedia with an entirely different wiki engine.
Arbitrary Area Filling in a Fast Procedure (1985)

Abstract A representation for area filling is described which allows shading of an area that may have both straight lines and circular arc edges and include holes within its boundaries. For efficiency an elimination algorithm which has a waiting line list and a currently active line list is adopted. For calculating intersections, a more time saving method is used. The algorithm and some practical examples are discussed. The main features of the algorithm are execution with high speed and less storage requirement. The program is written both in BASIC and FORTRAN 77 and could be executed on a microcomputer such as APPLE II, IBM‐P/C etc. The sample outputs shown in this paper are generated by the APPLE II with high efficiency.
Virtual Servants: Stereotyping Female Front‐Office Employees on the Internet (2005)

This article focuses on the service providers of the future: virtual assistants on the Internet. Recent technological developments, supported by intensive research on artificial intelligence, have enabled corporations to construct ‘virtual employees’ who can interact with their online customers. The number of virtual assistants on the Internet continues to grow and most of these new service providers are human‐like and female. In this article I profile virtual employees on the Internet — who they are, what they do and how they present themselves. I demonstrate that the Internet suffers from the same gender stereotyping characteristic of customer services in general and that the unreflective choice of female images is, at the minimum, a symbolic reinforcement of the real circumstances of gender divisions in customer service.
Iron carbonyl complexes of some perfluoromethyl-substituted poly(phosphines) (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIron carbonyl complexes of some perfluoromethyl-substituted poly(phosphines)A. H. Cowley and K. E. HillCite this: Inorg. Chem. 1973, 12, 6, 1446–1448Publication Date (Print):June 1, 1973Publication History Published online1 May 2002Published inissue 1 June 1973https://pubs.acs.org/doi/10.1021/ic50124a053https://doi.org/10.1021/ic50124a053research-articleACS PublicationsRequest reuse permissionsArticle Views24Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Proliferative effects of insulin analogues on mammary epithelial cells (2008)

Structural modification of insulin results in the generation of insulin analogues that show altered binding affinities to the insulin receptor and/or IGF-I receptor. As a consequence these insulin analogues may have increased mitogenic potency. Nine benign or malignant human mammary epithelial cells, which show different insulin receptor and IGF-I receptor expression patterns, were studied regarding mitogenicity of insulin and insulin analogues. Only insulin glargine showed a significantly higher proliferative effect on MCF-7 breast cancer cells compared to regular insulin among a panel of short- or long-acting insulin analogues, that are in clinical use.
Liposomal Meningococcal B Vaccination: Role of Dendritic Cell Targeting in the Development of a Protective Immune Response (2003)

ABSTRACT The effect of targeting strategies for improving the interaction of liposomal PorA with dendritic cells (DC) on the immunogenicity of PorA was investigated. PorA, a major antigen of Neisseria meningitidis , was purified and reconstituted in different types of (targeted) liposomes, i.e., by using mannose or phosphatidylserine as targeting moieties, or with positively charged liposomes. We studied the efficiency of liposome uptake and its effect on the maturation of and interleukin 12 (IL-12) production by murine DC. Moreover, mice were immunized subcutaneously to study the localization and immunogenicity of PorA liposomes. Uptake of liposomes by DC was significantly increased for targeted liposomes and resulted in the maturation of DC, but to various degrees. Maturation markers (i.e., CD80, CD86, major histocompatibility complex class II, and CD40) showed enhanced expression on DC incubated with targeted PorA liposomes relative to those incubated with nontargeted PorA liposomes. Moreover, only the uptake of targeted PorA liposomes induced production of IL-12 by DC, with levels similar to those produced by lipopolysaccharide (LPS)-pulsed DC. Mannose-targeted PorA liposomes administered subcutaneously had an increased localization in draining lymph nodes compared to nontargeted PorA liposomes. Liposomes in draining lymph nodes interacted preferentially with antigen-presenting cells, an effect that was enhanced with targeted PorA liposomes. Immunization studies showed an improvement of the bactericidal antibody response (i.e., increased number of responders) generated by targeted PorA liposomes compared to that generated by nontargeted ones or LPS-containing outer membrane vesicles. In conclusion, the use of targeted PorA liposomes results in an improved uptake by and activation of DC and an increased localization in draining lymph nodes. These effects correlate with an enhanced immune response toward the vaccine.
Painful Neuromas (1999)

There are numerous methods cited in the literature on the treatment of painful neuroma. Nonsurgical methods range from injections with various materials into the nerve end to desensitization of nerve pain conduction pathways. Some surgical treatments aim to alter the environment of the amputated nerve end by transposing it into muscle or bone, others have designed various flaps to protect truncated nerve ends from scar tissue, and still others try to "cap" the nerve with silicon, a nerve graft, or epineurium to prevent nerve regeneration. All of these methods have proved efficient. However, none of these methods work universally. The authors review the common treatments for painful neuromas. In addition, they review the preliminary results of the extended autologous venous nerve conduit as a novel technique of treating painful neuromas. They also report recent investigations into the pathophysiology of injured nerves.
Lithium-ion conducting oxide single crystal as solid electrolyte for advanced lithium battery application (2018)

Today, all-solid-state secondary lithium-ion batteries have attracted attention in research and development all over the world as a next-generation energy storage device. A key material for the all-solid-state lithium batteries is inorganic solid electrolyte, including oxide and sulfide materials. Among the oxide electrolytes, garnet-type oxide exhibits the highest lithium-ion conductivity and a wide electrochemical potential window. However, they have major problems for practical realization. One of the major problems is an internal short-circuit in charging and discharging. In the polycrystalline garnet-type oxide electrolyte, dendrites of lithium metal easily grow through the void or impurity in grain boundaries of the sintered body, which causes serious internal short-circuits in the battery system. To solve these problems, we present an all-solid-state battery system using a single-crystal oxide electrolyte. We are the first to successfully grow centimeter-sized single crystals of garnet-type by the floating zone method. The single-crystal solid electrolyte exhibits an extremely high lithium-ion conductivity of 10<sup>-3</sup> S cm<sup>-1</sup> at 298 K. The garnet-type single-crystal electrolyte has an advantageous bulk nature to realize the bulk conductivity without grain boundaries such as in a sintered polycrystalline body, and will be a game-changing technology for achieving highly safe advanced battery systems.
radEq Add-On Module for CFD Solver Loci-CHEM (2013)

Loci-CHEM to be applied to flow velocities where surface radiation due to heating from compression and friction becomes significant. The module adds a radiation equilibrium boundary condition to the computational fluid dynamics (CFD) code to produce accurate results. The module expanded the upper limit for accurate CFD solutions of Loci-CHEM from Mach 4 to Mach 10 based on Space Shuttle Orbiter Re-Entry trajectories. Loci-CHEM already has a very promising architecture and performance, but absence of radiation equilibrium boundary condition limited the application of Loci-CHEM to below Mach 4. The immediate advantage of the add-on module is that it allows Loci-CHEM to work with supersonic flows up to Mach 10. This transformed Loci-CHEM from a rocket engine- heritage CFD code with general subsonic and low-supersonic applications, to an aeroheating code with hypersonic applications. The follow-on advantage of the module is that it is a building block for additional add-on modules that will solve for the heating generated at Mach numbers higher than 10.
Low-order dynamic models for two coupled distillation columns (1999)

Nonlinear low-order models are developed for two coupled distillation columns separating a ternary mixture of methanol, ethanol and 1-propano!. The approach is based on well-known spatio temporal pattern formation phenomena in distillation processes also termed as nonlinear wave propagation. In a first step, the energy balance is neglected assuming constant molar flow rates in each column section. Further, constant molar holdups are assumed. In a second step, the wave approach is extended for columns with variable flow rates and constant volumetric holdups. It is shown that these assumptions are more realistic for the process considered here. In both cases the low-order models are in good agreement with the corresponding reference models with constant and variable flow rates, respectively. Further, the reduction of the system order is considerable in both cases.