gebhart/patrepeval huggingface dataset:
Abstract 1097: MicroRNA expression in white blood cells of CML patients: Differential expression in responders and non-responders to imatinib (2012)

Abstract Introduction: Treatment of chronic myeloid leukemia (CML) with the BCR/ABL-inhibitor imatinib led to a tremendous progress of over-all survival. However therapy resistance in a significant proportion of patients remains a severe clinical problem. Aside mutations of the BCR/ABL gene, regulation of cellular transporters or downstream factors may contribute to non-response. We aimed to investigate micro-RNA expression profiles in peripheral leukocytes of 21 newly diagnosed CML patients without BCR/ABL-mutations in order to identify biomarkers to treatment-response of imatinib. Methods: Ten responders (molecular remission) and 11 non-responders were included. Expression of 667 microRNAs was analyzed using a TaqMan Low-Density Array system. Relative fold change of microRNAs between groups was calculated according to the 2^-ΔΔCt method. MicroRNAs with a fold change&amp;gt;2 and a P-value&amp;lt;0.01 were considered significant. MicroRNAs with minor expression (Ct-value&amp;gt;20) were not included. Putative targets of dysregulated microRNAs were considered, if predicted by at least three databases, and further analyzed using the DAVID-bioinformatic database. Results: Four microRNAs were significantly deregulated (miR-7, miR-744*, miR-616, miR-212) between responders and non-responders, when being treatment-naïve, predicted to have 97 potential targets. In depth target analysis showed that transcription regulators (21% of the predicted targets) are highly enriched compared to normal gene expression background of humans. Pathway analysis revealed six genes involved in cancer pathways, whereas four of them are directly involved in the chronic myeloid leukemia pathway (SMAD4, NRAS, RB1, RAF1). In responders seven microRNAs were deregulated before and after therapy, whereas five other microRNAs were deregulated within the group of non-responders. Three deregulated microRNAs were identified in both groups. Most predicted target genes are involved in MAPK signaling and exocytosis, 13% of the targets were predicted to be transcription regulators, and 18% cellular (especially uptake) transporters. Conclusion: We identified distinct microRNA pattern comparing blood samples of responders and non-responders prior to imatinib therapy. Predicted target genes were primarily transcription factors and oncogenes. In contrast, transporters and exocytotic pathways are in addition frequent targets of microRNAs deregulated after imatinib therapy. The suitability as biomarkers for prediction of imatinib-response requires further confirmation. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1097. doi:1538-7445.AM2012-1097
Design of Variable Reflectivity Output Coupler for High Power CO2 Lasers (1998)

In order to increase the laser power of CO 2 lasers the active volume has to be enlarged. For CO 2 lasers there are two possibilities: to increase either the resonator length or the tube diameter. A larger tube diameter means a higher Fresnel number and the laser will be most likely operated with an unstable resonator configuration, which extracts a beam with higher beam quality than a multimode stable resonator. Increasing the resonator length while the diameter remains constant leads to a smaller Fresnel number and a stable resonator configuration becomes reasonable. However, in both cases diffraction effects will be observed due to the limiting apertures, which affect the beam quality especially for high laser powers. To reduce these effects so called variable reflectivtiy mirrors (VRM) have been introduced (1) and investigated especially for solid state lasers (2). So far most investigations have been carried out on unstable VRM-resonators.
Role of vascular endothelial growth factor C in classical Hodgkin lymphoma (2014)

We performed three different studies to obtain additional data on the biological and prognostic role of vascular endothelial growth factor C (VEGFC) in classical Hodgkin lymphoma (cHL): (1) serum VEGFC levels were measured by enzyme-linked immunosorbent assay in 80 patients; (2) immunohistochemical VEGFC expression in tumor tissue was evaluated using a case-control study in 62 patients; (3) gene expression of VEGFC was investigated in vitro in six cHL cell lines, and was compared with expression in inflammatory lymph nodes. Higher serum VEGFC levels were found in patients with cHL than in healthy volunteers (median, 7675 vs. 1358 pg/mL, p < 0.001) and high VEGFC expression in tissue predicted worse progression-free survival at 7 years (41% vs. 91%, p < 0.0001). VEGFC expression in all Hodgkin lymphoma cell lines was higher than the mean expression level in inflammatory lymph nodes. Our data suggest that Hodgkin and Reed-Sternberg cells produce VEGFC, and VEGFC expression could be a novel prognostic factor in cHL.
Long-term outcomes in patients with AML achieving first complete remission: confronting the double-hit of survivorship (2020)

In 1980, the 5-year overall survival rate for patients with acute myeloid leukemia (AML) in the United States (US) was a truly dismal 7%. Over 30 years later in 2012, approximately 30% of adult pat...
An effective cell-penetrating antibody delivery platform (2019)

Despite their well-recognized success in the clinic, antibodies generally do not penetrate cellular membranes to target intracellular molecules, many of which underlie incurable diseases. Here we show that covalently conjugating phosphorothioated DNA oligonucleotides to antibodies enabled their efficient cellular internalization. Antibody cell penetration was partially mediated by membrane potential alteration. Moreover, without an antigen to bind, intracellular levels of the modified antibodies underwent cellular clearance, which involved efflux and lysosomal degradation, enabling detection of intended intracellular molecules as tested in fibroblasts, tumor cells, and T cells. This target-dependent cellular retention of modified antibodies extended to in vivo studies. Both local and systemic administrations of low doses of modified antibodies effectively inhibited intracellular targets, such as transcription factors Myc, interferon regulatory factor 4, and tyrosine-protein kinase SRC, and expression of their downstream genes in tumors, resulting in tumor cell apoptosis and tumor growth inhibition. This simple modification enables the use of antibodies to detect and modulate intracellular molecules in both cultured living cells and in whole animals, forming the foundation for a new paradigm for antibody-based research, diagnostics, and therapeutics.
Effect of dietary vitamin C and vitamin E on tissue lipid peroxidation of guinea pigs fed with oxidized oil. (1986)

Eighteen male guinea pigs were divided into two groups and fed for 2 weeks with a diet containing 284 ppm of vitamin C and 50 ppm of vitamin E. One group received autoxidized linseed oil (40mg/animal/day) three times during the feeding. Compared with the animals not given the oxidized oil, the body weight gains, liver ascorbic acid levels and α-tocopherol contents were significantly decreased and liver lipid peroxidation was greatly stimulated in the animals dosed with the oxidized oil. In the next experiment, forty male guinea pigs were divided into five groups; only one group received a diet containing 1000 ppm of vitamin C and 50 ppm of vitamin E without oxidized oil, and the other four groups received graded diets of 1000-3000 ppm of vitamin C and 50-200ppm of vitamin E for 35 days. To the latter four groups, the oxidized oil (24 mg/animal/day) was orally administered 12 times during the feeding period. The tocopherol contents of the kidney and lung were found to be lower in the animals fed with a 3000 ppm-vitamin C and 200 ppm-vitamin E diet than those fed with a 1000 ppm-vitamin C and 200 ppm-vitamin E diet. The lung ascorbic acid content was lower in animals fed with a 3000 ppm-vitamin C and 200 ppm-vitamin E diet than in those fed with a 3000 ppm-vitmin C and 50 ppm-vitamin E diet. The effect of dietary vitamins C and E on tissue lipid peroxidation was different among the individual organs. From the results obtained by chemiluminescence analyses, the lipid peroxidation caused in the liver, kidney and heart was recognized to be suppressed effectively when 200 ppm-vitamin E diets were given. Antioxidative synergism of vitamin E (200 ppm) and C (3000 ppm) was found in the liver and lung as estimated by the chemiluminescence.
Broadband Service Delivery: CY.T.A. ADSL Field Trial Experience (2000)

The growing demand in broadband services, be it for example the Fast Internet, Multimedia Services, Video Broadcast Services, calls for the re-examination of the technology platforms deployed in a future-proof access network Certainly, the cabled medium to carry such services is an all optical Fiber To The Home (FTTH) network Such a high speed access network could theoretically provide the complete range of all currently known and new services being discussed for the residential subscribers and business customers. However, due to the cost of deploying such technology and due to the immaturity of the technology, incumbents are driven towards harnessing existing copper infrastructures to the maximum extent possible. In this paper, we present the work carried out by the Cyprus Telecommunications Authority (CYTA) in the field of broadband service delivery over ADSL and highlight critical issues for doing so in a field trial environment, whereby multi-vendor equipment and multiple Service Provider presence are a reality. In the field trial, both Full Rate ADSL and ADSL Lite are deployed.
Electrocardiographic aspects of deep dives in elite breath-hold divers. (2013)

The cardiac diving response, 12-lead electrocardiogram (ECG) and the prevalence, time of onset, and possible associations of cardiac arrhythmias were examined during deep breath-hold (BH) dives. Nine elite BH divers (33.2 +/- 3.6 years; mean +/- SD) performed one constant-weight dive of at least 75% of their best personal performance (70 +/- 7 meters for 141 +/- 22 seconds) wearing a 12-lead ECG Holter monitor. Diving parameters (depth and time), oxygen saturation (SaO2), blood lactate concentration and ventilatory parameters were also recorded. Bradycardia during these dives was pronounced (52.2 +/- 12.2%), with heart rates dropping to 46 +/- 10 beats/minute. The diving reflex was strong, overriding the stimulus of muscular exercise during the ascent phase of the dive for all divers. Classical arrhythmias occurred, mainly after surfacing, and some conduction alterations were detected at the bottom of the dives. The BH divers did not show any right shift of the QRS electrical axis during their dives.
Purification and Characterization of a Membrane‐Bound Enkephalin‐Forming Carboxypeptidase, “Enkephalin Convertase” (1984)

Enkephalin convertase, the enkephalin-synthesizing carboxypeptidase B-like enzyme, has been purified to apparent homogeneity from bovine pituitary and adrenal chromaffin granule membranes. The membrane-bound enkephalin convertase can be solubilized in high yield with 0.5% Triton X-100 in the presence of 1 M NaCl. Extensive purification is achieved by affinity chromatography with p-aminobenzoyl-L-arginine linked to Sepharose 6B. Enzyme purified from both pituitary and adrenal chromaffin granule membranes shows a single band by sodium dodecyl sulfate polyacrylamide gel electrophoresis with an apparent molecular weight of 52,500, whereas enkephalin convertase purified from soluble extracts of these tissues has an apparent molecular weight of 50,000. The regional distribution of the membrane-bound enzyme in the rat brain differs from that of the soluble enzyme. While the soluble enzyme shows 10-fold variations, resembling somewhat the enkephalin peptides, membrane-bound enkephalin convertase is more homogeneously distributed throughout the brain. In rat pituitary glands, membrane-bound enzyme activity is similar in the anterior and posterior lobes, whereas the soluble enzyme is enriched in the anterior lobe. Membrane-bound and soluble forms of enkephalin convertase isolated from either bovine pituitary glands or adrenal chromaffin granules show identical substrate and inhibitor specificities. As with the soluble enzyme, membrane-bound enkephalin convertase hydrolyzes [Met]- and [Leu]enkephalin-Arg6 and -Lys6 to enkephalin, with no further degradation of the pentapeptide.
Challenges and Opportunities of Fats and Oils (2018)

ADVERTISEMENT RETURN TO ISSUEViewpointNEXTChallenges and Opportunities of Fats and OilsAlejandro G. Marangoni*Alejandro G. MarangoniDepartment of Food Science, University of Guelph, Guelph, Ontario N1G2W1, Canada*E-mail: [email protected]. Phone: +1 519-824-4120 ext. 45340.More by Alejandro G. Marangonihttp://orcid.org/0000-0002-3129-4473Cite this: J. Agric. Food Chem. 2018, 66, 13, 3257–3259Publication Date (Web):March 22, 2018Publication History Received5 February 2018Published online22 March 2018Published inissue 4 April 2018https://pubs.acs.org/doi/10.1021/acs.jafc.8b00659https://doi.org/10.1021/acs.jafc.8b00659in-briefACS PublicationsCopyright © 2018 American Chemical SocietyRequest reuse permissionsArticle Views648Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Food,Lipids,Plant derived food,Redox reactions,Salts Get e-Alerts
A Truncated Form of KlLsm4p and the Absence of Factors Involved in mRNA Decapping Trigger Apoptosis in Yeast (2003)

The LSM4 gene of Saccharomyces cerevisiae codes for an essential protein involved in pre-mRNA splicing and also in mRNA decapping, a crucial step for mRNA degradation. We previously demonstrated that the first 72 amino acids of the Kluyveromyces lactis Lsm4p (KlLsm4p), which contain the Sm-like domains, can restore cell viability in both K. lactis and S. cerevisiae cells not expressing the endogenous protein. However, the absence of the carboxy-terminal region resulted in a remarkable loss of viability in stationary phase cells (). Herein, we demonstrate that S. cerevisiae cells expressing the truncated LSM4 protein of K. lactis showed the phenotypic markers of yeast apoptosis such as chromatin condensation, DNA fragmentation, and accumulation of reactive oxygen species. The study of deletion mutants revealed that apoptotic markers were clearly evident also in strains lacking genes involved in mRNA decapping, such as LSM1, DCP1, and DCP2, whereas a slight effect was observed in strains lacking the genes DHH1 and PAT1. This is the first time that a connection between mRNA stability and apoptosis is reported in yeast, pointing to mRNA decapping as the crucial step responsible of the observed apoptotic phenotypes.
An Integrated One-Cycle Control Buck Converter With Adaptive Output and Dual Loops for Output Error Correction (2004)

An integrated adaptive-output switching converter is presented. This converter adopts one-cycle control for fast line response and dual error correction loops for tight load regulation. A dc level shifting technique is proposed to eliminate the use of negative supply and reference voltages in the controller and make the design compatible with standard digital CMOS process. The design accommodates both continuous and discontinuous conduction operations. To further enhance the efficiency, dynamic loss control on the power transistors is proposed to minimize the sum of switching and conduction losses. The design can be extended to other dc-dc and ac-dc conversions. The prototype of the buck converter was fabricated with a standard 0.5-/spl mu/m digital CMOS process. Experimental results show that the converter is well regulated over an output range of 0.9-2.5 V, with a supply voltage of 3.3 V. The tracking speeds are 12.25 /spl mu/s/V for a 1.6-V step-up output change and 13.75 /spl mu/s/V for a 1.6-V step-down output change, respectively, which are much faster than existing counterparts. Maximum efficiency of 93.7% is achieved and high efficiency above 75% is retained over an output power ranging from 10 to 450 mW.
Group 2 innate lymphoid cells are numerically and functionally deficient in the triple transgenic mouse model of Alzheimer’s disease (2021)

Our results collectively indicate that numerical and functional deficiency of ILC2 might contribute to the cognitive impairment of 3xTg-AD mice.
Plasmon and periodicity assisted scattering and absorption of light by finite nanowire gratings (2012)

International audience
Use of a 90° phase shift detector and sampled-data loop filter in PLL (2003)

A modified architecture of a PLL that permits a fully monolithic integration is proposed. The key features of the architecture are the use of a phase frequency detector (PFD) operating on a 90/spl deg/ phase shift and a switched capacitor filter instead of a passive filter. The PFD operates with relatively long current pulses, thus reducing non-ideality with respect to the conventional PFD. A behavioral model in Matlab-Simulink has been used for different PFDs and low pass filters (LPF) used in the proposed architecture. Results show that the proposed solution achieves the same performances as a conventional solution with 11 times less capacitance.
Entrapment and controlled release of xenon in Cd2+-exchanged zeolite rho (1993)

D. R. Corbin, L. Abrams, G. A. Jones, M. L. Smith, C. R. Dybowski, J. A. Hriljac and J. B. Parise, J. Chem. Soc., Chem. Commun., 1993, 1027 DOI: 10.1039/C39930001027
Characteristics of selective PMMA etching for forming a PS mask (2013)

The characteristics of poly(methyl methacrylate) (PMMA) etching of self-assembled poly(styrene-<i>block</i>-methyl methacrylate) (PS-<i>b</i>-PMMA) thin film for forming a polystyrene (PS) mask were investigated. In this investigation, first, the etching selectivity of PMMA to PS under argon- and oxygen-plasma processes was evaluated. Higher selectivity was obtained in the case of argon plasma (3.9) compared to that of oxygen plasma (1.7). Second, to investigate the argon process in detail, the time dependence of etching depth was evaluated. It was found that PMMA etching rate decreases by more than half after etching to a depth of around 15 nm. To investigate the mechanism of this decrease in PMMA etching rate, the surface composition of PMMA was measured by X-ray photoelectron spectroscopy (XPS). The XPS result revealed that the reduction of etching rate is caused by a depletion of oxygen by argon ions, and the depleted oxygen attaches to the PMMA film in air exposure. In accordance with these results, to compensate the decrease in oxygen concentration, oxygen was added to the argon plasma at a composition of 1%. As a result of this oxygen addition, constant PMMA etching rate was confirmed, even beyond etching depth of 50 nm. It is thus concluded from these results that a PS lamellar mask pattern with a pitch from 25.5 to 70 nm could be successfully formed by using selective PMMA etching.
Transfusion-Induced Hepatitis C Virus Infection without Detectable Antivirus Antibodies in the Recipient (1998)

A case of infection with hepatitis C virus is reported, caused by platelets donated in the window-phase of donor serocon-version. The recipient, a patient with acute myelogenous leukemia, had never developed any detectable antivirus antibodies; the infection could only be demonstrated by polymerase chain reaction.
Electroactive biofilms on surface functionalized anodes: The anode respiring behavior of a novel electroactive bacterium, Desulfuromonas acetexigens (2020)

Surface chemistry is known to influence the formation, composition, and electroactivity of electron-conducting biofilms. However, understanding of the evolution of microbial composition during biofilm development and its impact on the electrochemical response is limited. Here we present voltammetric, microscopic and microbial community analysis of biofilms formed under fixed applied potential for modified graphite electrodes during early (90 h) and mature (340 h) growth phases. Electrodes modified to introduce hydrophilic groups (-NH<sub>2</sub>, -COOH and -OH) enhance early-stage biofilm formation compared to unmodified or electrodes modified with hydrophobic groups (-C<sub>2</sub>H<sub>5</sub>). In addition, early-stage films formed on hydrophilic electrodes are dominated by the gram-negative sulfur-reducing bacterium Desulfuromonas acetexigens while Geobacter sp. dominates on -C<sub>2</sub>H<sub>5</sub> and unmodified electrodes. As biofilms mature, current generation becomes similar, and D. acetexigens dominates in all biofilms irrespective of surface chemistry. Electrochemistry of pure culture D. acetexigens biofilms reveal that this microbe is capable of forming electroactive biofilms producing considerable current density of > 9 A/m<sup>2</sup> in a short period of potential-induced growth (~19 h following inoculation) using acetate as an electron donor. The inability of D. acetexigens biofilms to use H<sub>2</sub> as a sole source electron donor for current generation shows promise for maximizing H<sub>2</sub> recovery in single-chambered microbial electrolysis cell systems treating wastewaters.