1681 PURINERGIC P2X1 RECEPTOR GENE EXPRESSION IN DETRUSOR TISSUE FROM PATIENTS WITH BLADDER OUTLET OBSTRUCTION (BOO) AND DETRUSOR INSTABILITY (DI) (2010)

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Non-Neurogenic Voiding Dysfunction II1 Apr 20101681 PURINERGIC P2X1 RECEPTOR GENE EXPRESSION IN DETRUSOR TISSUE FROM PATIENTS WITH BLADDER OUTLET OBSTRUCTION (BOO) AND DETRUSOR INSTABILITY (DI) Christian Hampel, Jon Jones, Joachim W. Thüroff, and Christoph Wiesner Christian HampelChristian Hampel More articles by this author , Jon JonesJon Jones More articles by this author , Joachim W. ThüroffJoachim W. Thüroff More articles by this author , and Christoph WiesnerChristoph Wiesner More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1506AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Purinergic P2X1 receptors on the detrusor muscle mediate efferent ATP driven detrusor excitability. In case of patients with LUTS refractory to common anticholinergic therapy, inhibition of P2X1 receptors may be an pharmacological target. We investigate the molecular gene expression of purinergic P2X1 receptors in human detrusor tissue associated with BOO and/or DI METHODS 73 detrusor specimens of prostate cancer patients were obtained from the anterior bladder wall during radical retropubic prostatectomy after the patients had undergone preoperative cystometry and pressure-flow-studies. According to their specific urodynamic pattern, the patients were stratified into four groups (normal: n=23, obstructive non-irritative: n=25, irritative non-obstructive: n=10, obstructive irritative: n=15). After extraction of tissue RNA, a P2X1 primer set was designed for 2-step Light Cycler PCR. Amplification values were equalized for RNA quality (GAPDH amplification as external standard), correlated with the specimen specific urodynamic group and statistically analysed (Mann-Whitney-U test). RESULTS Overall, the changes of P2X1 receptor gene expression in correlation with the urodynamic findings were insignificant (Tab. 1). However, when all irritative patients were compared to all non-irritative patients (regardless of their obstruction status), the decrease of P2X1 gene expression reached statistical significance (Irritative group: 0.91 ± 0.54, Non-Irritative group: 1.11 ± 0.5; p<0.05). Looking at the obstruction only there was no significant difference between the obstructive group (1.01 ± 0.59) and the non-obstructive group (1.06 ± 0.43). Tab. 1. Quantitative RT-PCR results of P2X1 purinergic receptor gene expression in correlation with urodynamic pattern (mean ± SD) P2X1/GAPDH amplification ratio Obstructive Urodynamics Non-obstructive Urodynamics Irritative Urodynamics 1.05±0.71 0.98±0.3 Non-irritative Urodynamics 1.1±0.54 1.09±0.48 CONCLUSIONS As P2X1 receptors act on the efferent control of bladder function, inhibition of those receptors might be a therapeutic option, although the changes in receptor gene expression are not too impressive. Severe side effects due to the ubiquitous presence of purinergic receptors will limit the use of P2X1 antagonists. However, since more and more attention is drawn on the afferent limb of the micturition reflex as the predominant cause for LUTS, further investigation of the changes of P2X2 or P2X3 receptor gene expression may be worthwhile. Mainz, Germany© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183 Issue 4S April 2010 Page: e649-e650 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.Metrics Author Information Christian Hampel More articles by this author Jon Jones More articles by this author Joachim W. Thüroff More articles by this author Christoph Wiesner More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...