gebhart/patrepeval huggingface dataset:
Specific and Rapid Diagnosis of Edwardsiella tarda by a Novel Loop-Mediated Isothermal Amplification Targeting the Upstream Region of hlyb Gene (2013)

Edwardsiella tarda has become one of the most severe pathogens in aquaculture industries throughout the world; therefore, a specific and rapid identification method for this bacterium is urgently needed. In the present study, a novel loop-mediated isothermal amplification (LAMP) was developed by targeting the upstream region of the hlyb gene of E. tarda, which was then named as UH-LAMP. The Mg(2+) concentrations, the reaction temperature, and the reaction time of UH-LAMP were optimized to 10 mM, 65°C, and 45 min, respectively. The detection limit of the UH-LAMP was 100-times higher than that of conventional polymerase chain reaction (10 versus 1000 CFU/test). Furthermore, the new UH-LAMP assay showed no cross-reactivity to the E. ictaluri belonging to the other species in the genus Edwardsiella. The high specificity of the assay was also confirmed by testing the nine strains of E. tarda collected from different geographical locations and the other 20 bacteria species. The assay can be performed in a simple water bath or a heat block and the detection result can be visualized by adding a fluorescent reagent to the reaction mixture. Taken together, our preliminary results indicate that this UH-LAMP assay provided a rapid, sensitive, and species-specific diagnostic tool for E. tarda and can easily be applied for the diagnosis under clinical or onsite conditions.
A T cell leukemia line produces factor(s) that render rat neutrophils cytotoxic. (1986)

Proteose-peptone-induced intraperitoneal neutrophils from rats were activated in terms of tumor cytotoxicity by pretreatment with culture supernatants from a human T cell leukemia line, Jurkat (culture sup). Culture sup-treated neutrophils showed cytotoxicity against various tumor cell lines. The cytotoxicity of culture sup-treated neutrophils was dependent on the number of neutrophils and the concentration of culture sup. Cytostasis by activated neutrophils was observed very early in the assay incubation period (within 6 hr), but cytolysis first occurred at 24 hr after the start of incubation. Factor(s) in culture sup responsible for the activation of cytotoxic neutrophils were stable to temperature and pH treatments, and their molecular weight was higher than 10,000. The responsible factor(s) for activation of cytotoxic neutrophils were different from interleukin-2, serum-derived factor, and bacterial lipopolysaccharide.
The impact of intraocular pressure on elastic wave velocity estimates in the crystalline lens (2016)

Intraocular pressure (IOP) is believed to influence the mechanical properties of ocular tissues including cornea and sclera. The elastic properties of the crystalline lens have been mainly investigated with regard to presbyopia, the age-related loss of accommodation power of the eye. However, the relationship between the elastic properties of the lens and IOP remains to be established. The objective of this study is to measure the elastic wave velocity, which represents the mechanical properties of tissue, in the crystalline lens ex vivo in response to changes in IOP. The elastic wave velocities in the cornea and lens from seven enucleated bovine globe samples were estimated using ultrasound shear wave elasticity imaging. To generate and then image the elastic wave propagation, an ultrasound imaging system was used to transmit a 600 µs pushing pulse at 4.5 MHz center frequency and to acquire ultrasound tracking frames at 6 kHz frame rate. The pushing beams were separately applied to the cornea and lens. IOP in the eyeballs was varied from 5 to 50 mmHg. The results indicate that while the elastic wave velocity in the cornea increased from 0.96 ± 0.30 m s<sup>-1</sup> to 6.27 ± 0.75 m s<sup>-1</sup> as IOP was elevated from 5 to 50 mmHg, there were insignificant changes in the elastic wave velocity in the crystalline lens with the minimum and the maximum speeds of 1.44 ± 0.27 m s<sup>-1</sup> and 2.03 ± 0.46 m s<sup>-1</sup>, respectively. This study shows that ultrasound shear wave elasticity imaging can be used to assess the biomechanical properties of the crystalline lens noninvasively. Also, it was observed that the dependency of the crystalline lens stiffness on the IOP was significantly lower in comparison with that of cornea.
effect of the parameters of AlN/GaN/AlGaN and AlN/GaN/InAlN heterostructures with a two-dimensional electron gas on their electrical properties and the characteristics of transistors on their basis (2016)

The effect of the layer thickness and composition in AlGaN/AlN/GaN and InAlN/AlN/GaN transistor heterostructures with a two-dimensional electron gas on their electrical and the static parameters of test transistors fabricated from such heterostructures are experimentally and theoretically studied. It is shown that the use of an InAlN barrier layer instead of AlGaN results in a more than twofold increase in the carrier concentration in the channel, which leads to a corresponding increase in the saturation current. In situ dielectric-coating deposition on the InAlN/AlN/GaN heterostructure surface during growth process allows an increase in the maximum saturation current and breakdown voltages while retaining high transconductance.
Outcome of Irrigation and Debridement after Failed Two-Stage Reimplantation for Periprosthetic Joint Infection (2018)

Success rates of irrigation and debridement for failed two-stage procedures are similar to the success rates of irrigation and debridement in primary implant infections. According to the current paper, irrigation and debridement are an acceptable treatment for acute reinfections after failed two-stage revision if performed within the first 30 postoperative days after failed two-stage procedure or if symptoms are present for less than 3 weeks in the presence of a susceptible organism.
Stimulatory effect of certain amino acids on xylanase production by alkalophilic Bacillus sp. (1987)

We reported that the addition of glycine to the culture medium increased the production of intracellular enzymes and their release by various microorganisms.1>2) Therefore, glycine and various amino acids may also be effective for the production of extracellular enzymes. The present report describes the effects of glycine and amino acids on the production of xylanase, an extracellular enzyme, by alkalophilic Bacillus No. C-125. The xylanase of this strain was purified and the properties of the enzyme were studied.3) As to the effects of glycine on extracellular
Rancang Bangun Home Automation Berbasis Ethernet Shield Arduino (2020)

AbstrakHome Automation system adalah sistem otomatisasi rumah yang dapat membuat rumah menjadi lebih hemat dengan penggunaan energi listrik yaitu dengan cara meng-on/off-kan berbagai peralatan seperti lampu , ac, kipas angin, tv, pompa air, motor pagar, motor garasi, motor gorden dan lain-lain. Home automation pada penelitian ini menggunakan Ethernet shield Arduino, empat buah lampu, dan sensor DHT22 untuk mengkontrol temperatur dan kelembaban. Home Automation yang telah dibuat dapat bekerja baik dan lancar dengan kecepatan respon waktu rata-rata 2 detik antara saat penekanan tombol saklar ON/OFF pada tampilan broswer dengan rangkaian home automation. Dan hasil pengujian pada sensor DHT22 memiliki ketelitian sebesar 98.65% untuk pengukuran temperatur dan ketelitian sebesar 98.72% untuk pembacaan kelembaban. AbstractThe home Automation system is a home automation system that makes your home more efcient than the use of electrical energy, giving you the ease to turn on / off various equipment such as electric lights, air conditioners, fans, TVs, water pumps, fencing motors, garage motors, Motor Curtains, etc. Home automation in this study uses an Arduino Ethernet shield, four lamps, and a DHT22 sensor to control temperature and humidity. The home automation test results have a response time of 2 seconds between pressing the ON / OFF switch on the browser display and the home automation device circuit. And the test results on the DHT 22 sensor have an accuracy of 98.65% for the measurement of air temperature and accuracy of 98.72% for the reading of air humidity
Clinical and pathological features of solid pseudopapillary neoplasm (SPN) of the pancreas: A retrospective review of cases at Mayo Clinic. (2017)

e15757 Background: SPN is a rare neoplasia of the pancreas, defined by the World Health Organization (WHO) as an epithelial low-grade malignant neoplasm, with a high-grade malignant transformation potential. Literature in this subject is scarce and limited only to small retrospective series. Methods: This is a retrospective study aiming to characterize patients with the diagnosis of SPN at Mayo Clinic between 1992 and 2016. Demographics, clinical and pathological features, recurrence rate and overall survival were recorded. Malignant SPN was defined as per the WHO 2010 classification (Perineural invasion, angioinvasion or deep tissue invasion) and compared to Benign SPN. Results: 44 patients with SPN were identified. 39 patients (88.6%) were female. Median age at diagnosis was 27.5 (Range: 13-64). The most frequent presentation was abdominal pain (52.6%), whereas 36.8% were diagnosed based on incidental imaging findings. Majority (60.4%) of the tumors were located at the pancreatic tail, and 48.5% were solid-cystic lesions. Vascular and lymph node involvement were infrequent. 15 cases (30.23%) were classified as malignant. Tumor size was associated with malignant behavior (Table). Three patients had a metastatic presentation. All but 1 patient were treated with surgery. R1 resection was more frequent in malignant SPN, and 2 patients experienced disease recurrence. Two Stage IV patients were treated with surgery and experienced long term survival, one of them without evidence of disease. There was only 1 death recorded. Conclusions: SNP presents predominantly on young women. The prognosis is excellent even in presence of malignant features, and a complete surgery remains as the main treatment. [Table: see text]
Energy processing circuits for low-power applications (2009)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2009.
Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops (2022)

The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops are not tolerant to extreme climatic environments. The aim in the near future, in a world with hunger and an increasing population, is to breed and/or engineer crops to tolerate abiotic stress with a higher yield. Some crop varieties display a certain degree of tolerance, which has been exploited by plant breeders to develop varieties that thrive under stress conditions. Moreover, a long list of genes involved in abiotic stress tolerance have been identified and characterized by molecular techniques and overexpressed individually in plant transformation experiments. Nevertheless, stress tolerance phenotypes are polygenetic traits, which current genomic tools are dissecting to exploit their use by accelerating genetic introgression using molecular markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe plant mechanisms to sense and tolerate adverse climate conditions and examine and discuss classic and new molecular tools to select and improve abiotic stress tolerance in major crops.
Spatial relationship preserving character motion adaptation (2010)

This paper presents a new method for editing and retargeting motions that involve close interactions between body parts of single or multiple articulated characters, such as dancing, wrestling, and sword fighting, or between characters and a restricted environment, such as getting into a car. In such motions, the implicit spatial relationships between body parts/objects are important for capturing the scene semantics. We introduce a simple structure called an interaction mesh to represent such spatial relationships. By minimizing the local deformation of the interaction meshes of animation frames, such relationships are preserved during motion editing while reducing the number of inappropriate interpenetrations. The interaction mesh representation is general and applicable to various kinds of close interactions. It also works well for interactions involving contacts and tangles as well as those without any contacts. The method is computationally efficient, allowing real-time character control. We demonstrate its effectiveness and versatility in synthesizing a wide variety of motions with close interactions.
Orbicularis Oculi Myocutaneous Island Flap for Upper Eyelid Reconstruction (2012)

The cosmetic and functional outcomes of a reconstruction of an upper eyelid defect due to trauma or neoplasm are very important. This article reports the technique of an orbicularis oculi myocutaneous island flap for covering small- to moderate-sized defects of the upper eyelid. Between March 2008 and March 2010, 5 patients underwent surgery for an upper eyelid reconstruction. After tumor excision and debridement, the eyelid margin was closed directly before the reconstruction. The flap was designed just beside the defect considering the amount of tissue remaining for the reconstruction. The flap was elevated by splitting the muscle by the direction of its fibers without damaging the pedicle. The elevated flap was then transposed to the defect without tension.The orbicularis oculi myocutaneous flap is a single-stage reconstruction with reliable vascularity. It can be used to cover a defect without compromising the primary closure of the donor site and can be customized to reconstruct the defect with minimal tissue. Therefore, an orbicularis oculi myocutaneous island flap can be an alternative for a single-stage reconstruction of small- to moderate-sized defects of the upper eyelid.
Direct Conversion of Aldehydes to Acyl Azides Using tert‐Butyl Hypochlorite. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Low-Insertion-Loss Fin-Line Filters for Millimetre-Wave Applications (1981)

A design theory is described for low-insertion-loss fin-line filters with fin-line gap widths over the total height of the waveguide housing. The theory includes both higher order mode propagation and the finite thicknesses of the dielectric and the metallic fins. An evolution strategy synthesis method yields optimum design data for three- and five resonator-type fin-line filters with several substrate thicknesses. The midband frequencies chosen are about 13, 34, 66, and 75 GHz. Measured minimum insertion-losses in the passband are about 0.2 dB (13 GHz), 0.5 dB (34 GHz), and 1.9 dB (65 GHz) for three-resonator-type filters as an example.
Thermal Actuated Solid Tunable Lens (2006)

In this letter, the concept of driving tunable solid lens using microthermal actuator is presented. This microoptical device is composed of a flexible polydimethylsiloxane (PDMS) lens, silicon conducting ring, and silicon heater. The mismatching of coefficient of thermal expansion and stiffness between PDMS and silicon will lead to the deformation of polymer lens during heating, so as to further change its focal length. To demonstrate the feasibility of this approach, a microfabrication processes have been established to monolithically fabricate the present microoptical device. The typical experiment results show that the tunable focal length was up to 834 mum with an input current of 70 mA
Three-dimensional laser optoacoustic and laser ultrasound imaging system for biomedical research (2015)

In this work, we introduce an improved prototype of the imaging system that combines three-dimensional optoacoustic tomography (3D-OAT) and laser ultrasound tomography slicer (2D-LUT) to obtain coregistered maps of tissue optical absorption and speed of sound (SOS). The imaging scan is performed by a 360 degree rotation of a phantom/mouse with respect to a static arc-shaped array of ultrasonic transducers. A Q-switched laser system is used to establish optoacoustic illumination pattern appropriate for deep tissue imaging with a tunable (730-840 nm) output wavelengths operated at 10 Hz pulse repetition rate. For the LUT slicer scans, the array is pivoted by 90 degrees with respect to the central transducers providing accurate registration of optoacoustic and SOS maps, the latter being reconstructed using waveform inversion with source encoding (WISE) technique. The coregistered OAT-LUT modality is validated by imaging a phantom and a live mouse. SOS maps acquired in the imaging system can be employed by an iterative optoacoustic reconstruction algorithm capable of compensating for acoustic wavefield aberrations. The most promising applications of the imaging system include 3D angiography, cancer research, and longitudinal studies of biological distributions of optoacoustic contrast agents (carbon nanotubes, metal plasmonic nanoparticles, fluorophores, etc.).
Helium and neon in interplanetary dust particles (1994)

A summary is given of experiments performed to determine the amounts and isotopic composition of the helium and neon found in individual interplanetary dust particles collected in the Earth’s stratosphere. The role of step‐heating and pulse‐heating techniques for performing the extractions is discussed together with the application of the techniques to determination of the thermal history of the particles. The practicality of using these methods for distinguishing between particles of cometary and asteroidal origin is considered.
ChemInform Abstract: Regioselective Protonation of Allyllithium Compounds. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
High resolution traveltime imaging (1995)

It is well known that traveltime tomography does not provide sufficient image quality of the earth. There are two major reasons: (1) resolution in traveltime inversion critically depends on the completeness of the recording aperture; (2) the number of independent traveltime equations is usually much less than the number of unknowns. The latter situation leads to the existence of different solutions which perfectly suit the same traveltime equations. Correct solutions cannot then be distinguished unless independent conditions (constraints) are imposed.
On the Covalency of Silver–Fluorine Bonds in Compounds of Silver(I), Silver(II) and Silver(III) (2003)

Unconventional covalence: For the higher silver fluorides the silver–fluorine chemical bonding is significantly covalent, in contrast to the conventional “ionic” picture most frequently used in the chemistry of inorganic fluorides. For KAgF4, an Ag(III) fluoride, the Ag(4d) states (see picture) are more within the “ligand band” (60 %) than within the “metal band” (40 %) reflecting significant covalency of the AgF bond, and an electronic band with significant hole production within the anionic F(2p) states.
Triethylborane from Triethylaluminum and Borazines (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTriethylborane from Triethylaluminum and BorazinesW. G. Woods and A. L. McCloskeyCite this: Inorg. Chem. 1963, 2, 4, 861–863Publication Date (Print):August 1, 1963Publication History Published online1 May 2002Published inissue 1 August 1963https://pubs.acs.org/doi/10.1021/ic50008a048https://doi.org/10.1021/ic50008a048research-articleACS PublicationsRequest reuse permissionsArticle Views122Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Molecular diversity of Ca2+ channel alpha 1 subunits from the marine ray Discopyge ommata. (1993)

In many neurons, transmitter release from presynaptic terminals is triggered by Ca2+ entry via dihydropyridine-insensitive Ca2+ channels. We have looked for cDNAs for such channels in the nervous system of the marine ray Discopyge ommata. One cDNA (doe-2) is similar to dihydropyridine-sensitive L-type channels, and two cDNAs (doe-1 and doe-4) are similar to the subfamily of dihydropyridine-insensitive non-L-type channels. doe-4, which encodes a protein of 2326 aa, most closely resembles a previously cloned N-type channel. doe-1, which encodes a protein of 2223 aa, is a member of a separate branch of the non-L-type channels. Northern blot analysis reveals that doe-1 is abundant in the forebrain. doe-4 is more plentiful in the electric lobe and, therefore, may control neurotransmitter release in motor nerve terminals. These results show that the familial pattern of Ca(2+)-channel genes has been preserved from a stage in evolution before the divergence of higher and lower vertebrates > 400 million years ago. The cloning of these channels may be a useful starting point for elucidating the role of the Ca2+ channels in excitation-secretion coupling in nerve terminals.
Feasibility of CT angiography acquired with low tube voltage and low iodine retrospective evaluation of radiation dose, image quality and iodine load (2015)

Poster: ECR 2015 / C-1347 / Feasibility of CT angiography acquired with low tube voltage and low iodine retrospective evaluation of radiation dose, image quality and iodine load by: Bavaharan1, A. Bherwani2, R. Anand Kumar3; 1Trichy/IN, 2Mumbai/IN, 3Erode/IN
The Federal Reserve and Financial Regulation: The First Hundred Years (2013)

This paper surveys the role of the Federal Reserve within the financial regulatory system, with particular attention to the interaction of the Fed's role as both a supervisor and a lender-of-last-resort (LOLR). The institutional design of the Federal Reserve System was aimed at preventing banking panics, primarily due to the permanent presence of the discount window. This new system was successful at preventing a panic in the early 1920s, after which the Fed began to discourage the use of the discount window and intentionally create "stigma" for window borrowing -policies that contributed to the panics of the Great Depression. The legislation of the New Deal era centralized Fed power in the Board of Governors, and over the next 75 years the Fed expanded its role as a supervisor of the largest banks. Nevertheless, prior to the recent crisis the Fed had large gaps in its authority as a supervisor and as LOLR, with the latter role weakened further by stigma. The Fed was unable to prevent the recent crisis, during which its LOLR function expanded significantly. As the Fed begins its second century, there are still great challenges to fulfilling its original intention of panic prevention.