gebhart/patrepeval huggingface dataset:
Gene mapping study for constitutive skin color in an isolated Mongolian population (2012)

To elucidate the genes responsible for constitutive human skin color, we measured the extent of skin pigmentation in the buttock, representative of lifelong non-sun-exposed skin, and conducted a gene mapping study on skin color in an isolated Mongolian population composed of 344 individuals from 59 families who lived in Dashbalbar, Mongolia. The heritability of constitutive skin color was 0.82, indicating significant genetic association on this trait. Through the linkage analysis using 1,039 short tandem repeat (STR) microsatellite markers, we identified a novel genomic region regulating constitutive skin color on 11q24.2 with an logarithm of odds (LOD) score of 3.39. In addition, we also found other candidate regions on 17q23.2, 6q25.1, and 13q33.2 (LOD ≥ 2). Family-based association tests on these regions with suggestive linkage peaks revealed ten and two significant single nucleotide polymorphisms (SNPs) on the linkage regions of chromosome 11 and 17, respectively. We were able to discover four possible candidate genes that would be implicated to regulate human skin color: ETS1, UBASH3B, ASAM, and CLTC.
Practical Considerations in Operation and Scale-up of Spin-Filter Based Bioreactors for Monoclonal Antibody Production (1996)

Minimum spin-filter fouling and optimum cell retention at high specific perfusion rates are important for efficient operation of a spin-filter based continuous perfusion bioreactor. We examined the effect of operation conditions and spin-filter configuration on the performance of continuous perfusion bioreactors using a perfusion recycle scheme. This study showed that single cell suspensions foul a spin-filter screen, partially but irreversibly, in the early stages of the bioreactor run. A high perfusion rate and cell density contribute to screen fouling, while an increase in rotational velocity and screen surface area per reactor volume reduce screen fouling. An empirical model was developed to describe the effects of these parameters on the perfusion capacity of a spin-filter. Examination of screen samples by scanning electron microscopy confirmed that partial screen fouling occurs at relatively early stages of fermentation. Once the initial partial screen fouling has occurred, further fouling continues slowly due to cell growth on the screen surface, and this gradually leads to the overflow state. The rate of this gradual fouling phase probably depends upon the number of cells deposited on the screen surface during the initial partial fouling. The usefulness of this model was confirmed by successful scale-up of high-cell-density (> 10 x 10(6)/mL) long-term (> 30 days) continuous perfusion process for commercial scale production of monoclonal antibodies.
A new method to improve the efficiency of the heat flow path of a micro thermoelectric generator (2012)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Zheng Yuan, Katir Ziouche, Zahia Bougrioua, Pascale Godts, Tuami Lasri, Didier Leclercq; A new method to improve the efficiency of the heat flow path of a micro thermoelectric generator. AIP Conference Proceedings 26 June 2012; 1449 (1): 435–438. https://doi.org/10.1063/1.4731589 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Characterization of CpdC, a Large-Ring Lactone-Hydrolyzing Enzyme from Pseudomonas sp. Strain HI-70, and Its Use as a Fusion Tag Facilitating Overproduction of Proteins in Escherichia coli (2013)

There are few entries of carbon-carbon bond hydrolases (EC 3.7.1.-) in the ExPASy database. In microbes, these enzymes play an essential role in the metabolism of alicyclic or aromatic compounds as part of the global carbon cycle. CpdC is a ω-pentadecalactone hydrolase derived from the degradation pathway of cyclopentadecanol or cyclopentadecanone by Pseudomonas sp. strain HI-70. CpdC was purified to homogeneity and characterized. It is active as a dimer of 56,000 Da with a subunit molecular mass of 33,349. Although CpdC has the highest activity and reaction rate (kcat) toward ω-pentadecalactone, its catalytic efficiency favors lauryl lactone as a substrate. The melting temperature (Tm) of CpdC was estimated to be 50.9 ± 0.1°C. The half-life of CpdC at 35°C is several days. By virtue of its high level of expression in Escherichia coli, the intact CpdC-encoding gene and progressive 3'-end deletions were employed in the construction of a series of fusion plasmid system. Although we found them in inclusion bodies, proof-of-concept of overproduction of three microbial cutinases of which the genes were otherwise expressed poorly or not at all in E. coli was demonstrated. On the other hand, two antigenic proteins, azurin and MPT63, were readily produced in soluble form.
Enhanced Magnetic Properties of Graphene Coated with Fe2O3 Nanoparticles (2015)

Graphene, with its unique 2D nanostructure and excellent electrical, thermal, and mechanical properties, is considered an alternative to carbon nanotubes in nanocomposites. In this study, we present a one step approach for the deposition of iron oxide (Fe2O3) nanoparticles onto graphene sheets through solution mixture. The morphology, crystallinity, and magnetic properties of as-synthesized composites were investigated. It was shown that highly crystalline Fe2O3 nanoparticles were densely and uniformly coated on graphene surface. Magnetic measurements reveal that, as compared to weak diamagnetism of pristine graphene, graphene-Fe2O3 nanocomposites display ferromagnetic behavior with coercivity of 101 Oe, saturation magnetization of 12.6 emu g(-1), and remanent magnetization of 3.13 emu g(-1) at room temperature. The enhanced magnetic performance was attributed to the homogeneous dispersion of Fe2O3 nanoparticles in graphene matrix and such nanocomposites are promising materials for applications in magnetic media and energy storage.
On-orbit parameter and disturbance identification for Space Station (1985)

This paper describes recent efforts directed at the problem of on-orbit parameter and disturbance identification for the Space Station. Techniques are developed for identification of rigid body parameters, flexible body parameters, and quasi-static disturbances relevant to the Power Tower configuration currently under investigation by NASA. The paper concludes with a case study investigating the identification of modal frequencies in a 15 degree of freedom planar model of the Space Station under a variety of operational settings.
Ipilimumab and nivolumab in advanced hepatocellular carcinoma after failure of prior immune checkpoint inhibitor-based combination therapies: a multicenter retrospective study (2022)

This retrospective study demonstrates that combined ipilimumab and nivolumab can be effective and tolerable after prior ICI-based combination therapies and provides a rationale for the prospective clinical evaluation of this treatment sequencing.
HER‐2/neu expression in extramammary Paget disease: a clinicopathologic and immunohistochemistry study of 47 cases with and without underlying malignancy (2009)

Extramammary Paget disease (EMPD) is an infrequent skin cancer sometimes representing a secondary event caused by extension of an underlying carcinoma. Her-2/neu overexpression in breast cancer is correlated with a more aggressive behavior, but anti-Her-2/neu therapy improves survival in these patients. We investigated Her-2/neu expression by immunohistochemistry in cases of EMPD with and without underlying malignancy to try to correlate with tumor recurrence, progression and possible targeted therapy. Forty-seven cases were analyzed (6 from the scrotum, 7 perianal region, 1 axilla and 33 vulva). Two cases had invasive EMPD (one from vulva and one from scrotum). The overall Her-2/neu expression was 31.9%. Of the noninvasive EMPD of the vulva (32 cases), Her-2/neu was shown in 38%. The case of invasive vulvar EMPD was negative. All six scrotal EMPD lacked Her2/neu expression. Her-2/neu was expressed in two of seven perianal cases (33.3%). The EMPD on the axilla (one case) was negative. Eighteen cases had recurrence, and of these, 44.4% expressed Her-2/neu in the initial lesion. A high proportion of EMPD showed Her-2/neu expression (31.9%), indicating that these patients may benefit from targeted therapy. The proportion of positive cases was higher in lesions that had recurred at last follow up (44.4%), suggesting a more aggressive behavior.
In Vitro Study of Odor-Evoked Behavior in a Terrestrial Mollusk (2004)

To explore neural mechanisms how olfactory information is processed in the brain and finally converted into behavior, it would be useful to have isolated whole brains that include both olfactory organs and motor output. In the present study, we identified an in vitro index of odor-evoked behavior in the terrestrial mollusk Limax and also studied the modulation of this in vitro index of the behavior. We determined that shortening of the mantle muscles is one of the withdrawal responses selectively induced by aversive odors and that the shortening is mediated by a pair of parietal nerves. We also identified a motoneuron (named the posterior visceral neuron, p-VN) that projects to the parietal nerve and innervates the mantle muscles. When we applied various odors to the nose in these isolated molluscan brains, only aversive odors induced discharges in the p-VN. These results indicate that p-VN discharges can serve as an in vitro index of odor-induced aversive behavior. We also identified a novel serotonergic neuron (named the posterior cerebral serotonergic cell, p-CSC). Discharges in the p-CSC released serotonin to the tentacle ganglion (TG); serotonin in the TG then inhibited odor-induced discharges in the p-VN, the in vitro index of aversive behavior. These results suggest that the serotonergic system is involved in the regulation of approach and avoidance behavior in Limax.
Model-Based Well Location Optimization – A Robust Approach (2017)

Abstract Well Location Optimization (WLO) plays a critical role in maximizing recovery from hydrocarbon reservoirs. Identification of well locations typically relies on expert subsurface knowledge and ad-hoc flow simulation scenarios. Such approaches, due to the complexity and uncertainty of subsurface descriptions, can easily miss highly profitable possibilities. This situation has motivated the development of model-based optimization workflows that can assist subsurface specialists in choosing improved well locations. In practice, model-based optimization workflows are implemented in a deterministic fashion and applied to low, mid and high simulation scenarios. The workflow proposed in this paper takes into account model uncertainty in the optimization outcomes. Deterministic WLO entails optimizing the easting and northing coordinates of wells with respect to an objective function (e.g. net present value or ultimate recovery). The workflow applies to fixed well trajectories. For each new proposed well location, there is a mapping of the new well connections to the reservoir simulation grid. The approach has shown resilience in handling realistic scenarios including non-uniform areal gridding, pinch out features, local grid refinements or tartan gridding, and non-uniform layering dimensions. Deterministic optimization techniques may anchor the results to particular portions of the uncertainty space, while robust optimization techniques propagate the effect of uncertain parameters on the optimization outcomes. The trade-off between the investigated span of uncertainty space and available computational capability needs to be made. Due to the continuous growth in computational capabilities, it is ever more attainable. Robust optimization incorporates the effect of uncertain parameters in the optimization process. It is a natural extension of the history matching process in brownfields, but it is also applicable to green fields where dynamic data is yet to be received. For such cases, a robust optimization workflow will yield outcomes that take into account the uncertainty of parameters described by a prior distribution, e.g. only conditioned to static data. The WLO workflow involves (i) executing deterministic WLO, (ii) examining the deterministic WLO results on a set of reservoir simulation models that capture the uncertainty space, and (iii) executing robust WLO. This workflow was tested with gradient-based optimization methods, derivative-free methods, and hybrid methods, the latter being the most optimal of the three. The robust implementation of this workflow handles realistic reservoir simulation scenarios by exploiting coarse-grained parallelism and has been applied successfully to field cases with up to fifty wells. In this paper, WLO is showcased on one synthetic case and one field case to illustrate the capabilities of the workflow.
Wind Tunnel Tests on a Nonaxisymmetric Projectile Shape at Mach Numbers 2.5 to 6.0. (1983)

Wing tunnel tests on spinning nonaxisymmetric projectile configurations at Mach numbers of 2.5 to 6.0 are reported. The projectile shapes are ogive- cylinder bodies which have been modified by machining three forward facing surfaces and three rearward facing surfaces on the projectile. The machined surfaces are twisted at a rate corresponding to a typical rifling twist. The forward facing surfaces produce a helical or corkscrew type effect and the rearward surfaces form a nonconical boattail and a triangular base. The objective of the test program was to study shapes which have improved stability characteristics which require less gyroscopic stabilization. A more slender, longer L/d projectile can then be flown which has lower drag and shorter time of flight to a moving target.
Quantum oscillations in rotationally inelastic molecule–surface scattering: Energy dependence of transition probabilities (1988)

Exact quantum mechanical and quasiclassical trajectory results for rotationally inelastic N2 -corrugated surface collisions are compared over the energy range 0.01–0.04 eV. It is found that the degeneracy averaged, diffraction summed, rotationally inelastic transition probabilities display quantum oscillations.
Infrared photostimulation of the crista ampullaris (2011)

The present results show that the semicircular canal crista ampullaris of the toadfish, Opsanus tau, is sensitive to infrared radiation (IR) applied in vivo. IR pulse trains (∼1862 nm, ∼200 μs pulse⁻¹) delivered to the sensory epithelium by an optical fibre evoked profound changes in phasic and tonic discharge rates of postsynaptic afferent neurons. Phasic afferent responses to pulsed IR occurred with a latency of <8 ms while tonic responses developed with a time constant (τ) of 7 ms to 10 s following the onset or cessation of the radiation. Afferents responded to direct optical radiation of the sensory epithelium but did not respond to thermal stimuli that generated nearly equivalent temperature increases of the whole organ. A subset of afferent neurons fired an action potential in response to each IR pulse delivered to the sensory epithelium, at phase-locked rates up to 96 pulses per second. The latency between IR pulses and afferent nerve action potentials was much greater than synaptic delay and spike generation, demonstrating the presence of a signalling delay interposed between the IR pulse and the action potential. The same IR stimulus applied to afferent nerve axons failed to evoke responses of similar magnitude and failed to phase-lock afferent nerve action potentials. The present data support the hypothesis that pulsed IR activates sensory hair cells, thus leading to modulation of synaptic transmission and afferent nerve discharge reported here.
IEEE 802.1ah Mode for Ethernet Over MPLS (2006)

Ethernet has been widely deployed in metro-networks, and there are
more and more customers select Ethernet PW services. With the
deployment of IEEE 802.1ah network, there is a need to support 802.1ah
attachment circuit. Based on the two modes defined in RFC 4448, this
document introduces a new Ethernet encapsulation mode to support the
new AC type. This document also describes some scenarios and
procedures pertaining to the new mode. Some considerations relating to
multi-segment PW are also discussed.
A rapid enzymatic procedure for "fingerprinting" bacteria by using pattern recognition of two-dimensional fluorescence data. (1986)

We describe a rapid fluorescence technique for bacterial "fingerprinting," based on the differences in enzyme content and activity of various bacteria. Bacterial cells are incubated with a mixture of carefully chosen fluorogenic enzyme substrates to produce fluorophores with unique emission and excitation properties. The resulting two-dimensional fluorescence spectrum of the product mixture produces a pattern characteristic of the bacterium. A Fourier-transform-based pattern recognition algorithm is used for spectral matching and for differentiating visually similar spectra. This sensitive technique is potentially applicable to differentiation and identification of bacteria in clinical laboratories.
The role of Ki-67 in determining optimal chemotherapy in high grade neuroendocrine tumors. (2018)

4100 Background: High grade (HG) neuroendocrine tumors (NETs) are rare neoplasms with limited data about optimal treatment. This retrospective analysis was performed to determine the most efficacious chemotherapy regimen in HGNETs. Methods: A single center, retrospective review of patients (pts) who received at least one chemotherapy regimen for HGNET between June 1, 2012 and June 1, 2017 was conducted. Data collection included demographics, pathologic characteristics, imaging results and treatment data. Progression free survival (PFS) was defined as date from initial chemotherapy until date of disease progression based on imaging or death. Median overall survival (mOS) was also analyzed. Chemotherapy regimens included combination capecitabine and temozolomide (CAPTEM), platinum-based therapy (cisplatin or carboplatin) or other (fluorouracil, or capecitabine based chemotherapy). Response to treatment was evaluated using RECIST 1.1. Subset analysis was performed based on Ki-67 ( < 55% or ≥55%). Results: Fifty-five pts received chemotherapy. Median number of treatment for those with a Ki-67 of < 55% and ≥55% was 3 and 1, respectively. MOS was 18 months irrespective of chemotherapy regimen; mOS by Ki-67 was 40 months for Ki-67 < 55% versus 16 months for Ki-67 ≥ 55% (Logrank test p = 0.0016). Among all pts undergoing first line therapy, CAPTEM significantly improved PFS (p = 0.041). In a sub analysis, Ki-67 < 55 had a longer PFS with CAPTEM (12-mo), followed by other (7-mo) and platinum (4-mo). In pts with Ki-67 ≥ 55%, PFS was highest in platinum-based therapy (5-mo), followed by CAPTEM (2-mo) and other (1-mo). Kaplan-Meier 3- and 6-month PFS rates based on chemotherapy regimen for pts with Ki-67 < 55% and Ki-67 ≥55 are shown in the table. Conclusions: This study demonstrates optimal first line chemotherapy treatment is dependent on Ki-67. Pts with a Ki-67 < 55 and Ki-67 ≥55 have improved PFS with CAPTEM and platinum-based therapy, respectively. Ki-67 values should be considered when determining first line chemotherapy regimen among pts with HGNET. 1st Line Regimen N Median PFS (mo) 3-mo PFS rate by Ki-67 (%) 6-mo PFS rate by Ki-67 (%) < 55% ≥55% < 55% ≥55% CAPTEM 15 12 77 50 77 50 Other 5 7 67 50 67 50 Platinum-based 35 4 51 58 25 36 Overall 55 5 65 57 54 38
Reconstruction of multi-energy X-ray computed tomography images of laboratory mice (1999)

A new X-ray computed tomography (CT) system is being developed at Oak Ridge National Laboratory to image laboratory mice for the purpose of rapid phenotype screening and identification. One implementation of this CT system allows simultaneous capture of several sets of sinogram data, each having a unique X-ray energy distribution. The goals of this paper are to (1) identify issues associated with the reconstruction of this energy-dependent data and (2) suggest preliminary approaches to address these issues. Due to varying numbers of photon counts within each set, both traditional (filtered backprojection, or FBP) and statistical (maximum likelihood, or ML) tomographic image reconstruction techniques have been applied to the energy-dependent sinogram data. Results of reconstructed images using both algorithms on sinogram data (high- and low-count) are presented. Also, tissue contrast within the energy-dependent images is compared to known X-ray attenuation coefficients of soft tissue (e.g. muscle, bone, and fat).
Coenzyme Q. 151. Antimetabolites of coenzyme Q. 14. Quinolinequinone analogs which inhibit mitochondrial DPNH-oxidase and succinoxidase (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCoenzyme Q. 151. Antimetabolites of coenzyme Q. 14. Quinolinequinone analogs which inhibit mitochondrial DPNH-oxidase and succinoxidaseChristine M. Bowman, Frederick S. Skelton, Thomas H. Porter, and Karl FolkersCite this: J. Med. Chem. 1973, 16, 3, 206–209Publication Date (Print):March 1, 1973Publication History Published online1 May 2002Published inissue 1 March 1973https://pubs.acs.org/doi/10.1021/jm00261a008https://doi.org/10.1021/jm00261a008research-articleACS PublicationsRequest reuse permissionsArticle Views46Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Diffusion in gas separation membrane materials: A comparison and analysis of experimental characterization techniques (1998)

Typically, materials with high-performance transport properties such as zeolites, carbon molecular sieves, or hyper rigid polymers are inherently difficult or impossible to characterize by steady-state membrane permeation experiments used for conventional polymers. Diffusion coefficients determined by transient sorption, a measurement easily performed on brittle media, are analyzed here and compared to those determined by steady-state permeation/sorption and transient permeation for a glassy polymer and a carbon molecular sieve. Average and local diffusion coefficients are extrapolated to zero upstream partial pressure to eliminate effects caused by concentration dependence. Good agreement between the techniques was observed for the glassy polymer. On the other hand, carbon molecular sieves, possessing a more complex morphology, exhibit a greater difference in diffusion coefficients determined by the various techniques. Nevertheless, comparison of the analysis techniques is shown to provide potentially valuable insights into the morphological features of such carbon molecular sieves. © 1998 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 36: 1747–1755, 1998