gebhart/patrepeval huggingface dataset:
Gating the mechanical channel Piezo1 (2012)

Piezo1 is a eukaryotic cation-selective mechanosensitive ion channel. To understand channel function in vivo, we first need to analyze and compare the response in the whole cell and the patch. In patches, Piezo1 inactivates and the current is fit well by a 3-state model with a single pressure-dependent rate. However, repeated stimulation led to an irreversible loss of inactivation. Remarkably, the loss of inactivation did not occur on a channel-by-channel basis but on all channels at the same time. Thus, the channels are in common mechanical domain. Divalent ions decreased the unitary conductance from ~68 pS to ~37 pS, irrespective of the cation species. Mg and Ca did not affect inactivation rates, but Zn caused a 3-fold slowing. CytochalasinD (cytoD) does not alter inactivation rates or the transition to the non-inactivating mode but does reduce the steady-state response. Whole-cell currents were similar to patch currents but also had significant differences. In contrast to the patch, cytoD inhibited the current suggesting that the activating forces were transmitted through the actin cytoskeleton. Hypotonic swelling that prestressed the cytoskeleton and the bilayer greatly increased the sensitivity of both control and cytoD cells so there are two pathways to transmit force to the channels. In contrast to patch, removing divalent ions decreased the whole-cell current. The difference between whole cell and patch properties provide new insights into our understanding of the Piezo1 gating mechanisms and cautions against generalization to in situ behavior.
A Hybrid Structure Dual-Path Step-Down Converter With 96.2% Peak Efficiency Using 250-m$\Omega$ Large-DCR Inductor (2019)

A dual-path step-down converter (DPDC) is presented for achieving high power efficiency in the mobile power management ICs (PMICs). Adopting a hybrid structure using one inductor and one flying capacitor, the proposed DPDC supplies a load current via two parallel paths, relieved an intrinsic problem of the conventional buck converter (CBC) topology, which is a significant power loss from a large DCR of the inductor (R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DCR</sub> ). Therefore, DPDC achieves a high power efficiency and thus also reduces the heating problem, which is another critical issue in the mobile set. Moreover, DPDC can shrink the volume of the PMIC set with a low manufacturing cost by alleviating an RDCR specification of the inductor. In this paper, although a 250 mQ of large R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DCR</sub> inductor is used for our measurements, a 96.2% of peak efficiency was achieved and the power loss of total parasitic resistances can be reduced to up to 30% of that of CBC. Moreover, according to our measurement plots, it is verified that DPDC achieves the efficiency notably higher not only in a wide load current (I <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LOAD</sub> ) range but also in a wide conversion ratio (V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">OUT</sub> /V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">IN</sub> ) range, compared to CBC.
Parallel ray-tracing with a transactional DSM (2004)

Distributed shared memory (DSM) is a well-known alternative to explicit message passing and remote procedure call. Numerous DSM systems and consistency models have been proposed in the past. The Plurix project implements a DSM operating system (OS) storing data and code, within the DSM. Our DSM storage features a new consistency model (transactional consistency) combining restartable transactions with an optimistic synchronization scheme instead of relying on a hard to use weak consistency model. In this paper we evaluate our system for the first time with a real parallel application, a parallel ray-tracer. The measurements show that our DSM scales quite well for this application even though we are using a strong consistency model.
Mesenchymal Stromal Cells Expressing ErbB-2/neu Elicit Protective Antibreast Tumor Immunity In vivo, Which Is Paradoxically Suppressed by IFN-γ and Tumor Necrosis Factor-α Priming (2010)

It is unknown whether mesenchymal stromal cells (MSC) can regulate immune responses targeting tumor autoantigens of low immunogenicity. We tested here whether immunization with MSC could break immune tolerance towards the ErbB-2/HER-2/neu tumor antigen and the effects of priming with IFN-γ and tumor necrosis factor-α (TNF-α) on this process. BALB/c- and C57BL/6-derived MSC were lentivirally transduced to express a kinase-inactive rat neu mutant (MSC/Neu). Immunization of BALB/c mice with nontreated or IFN-γ-primed allogeneic or syngeneic MSC/Neu induced similar levels of anti-neu antibody titers; however, only syngeneic MSC/Neu induced protective neu-specific CD8(+) T cell responses. Compared to immunization with nontreated or IFN-γ-primed syngeneic MSC/Neu, the number of circulating neu-specific CD8(+) T cells and titers of anti-neu antibodies were observed to be decreased after immunizations with IFN-γ- plus TNF-α-primed MSC/Neu. In addition, syngeneic MSC/Neu seemed more efficient than IFN-γ-primed MSC/Neu at inducing a protective therapeutic antitumor immune response resulting in the regression of transplanted neu-expressing mammary tumor cells. In vitro antigen-presenting cell assays performed with paraformaldehyde-fixed or live MSC showed that priming with IFN-γ plus TNF-α, compared to priming with IFN-γ alone, increased antigen presentation as well as the production of immunosuppressive factors. These data suggest that whereas MSC could effectively serve as antigen-presenting cells to induce immune responses aimed at tumor autoantigens, these functions are critically regulated by IFN-γ and TNF-α.
Formic Acid and Methanol Concentrations in Death Investigations (2008)

Methanol ingestion results in the formation of formic acid, a toxic metabolite that can cause metabolic acidosis. Methanol toxicity is therefore dependent on the amount of methanol ingested, the nature of treatment received, elapsed time since ingestion, and the accumulation of formic acid. Both methanol and formic acid concentrations are determined at this laboratory using headspace gas chromatography. An examination of 12 fatalities attributed to methanol poisoning is presented. Six individuals were found deceased, and their postmortem methanol and formic acid concentrations ranged from 84 to 543 mg/dL and 64 to 110 mg/dL, respectively. In the other six individuals, hospital treatment such as bicarbonate, ethanol infusion, and hemodialysis was administered. Antemortem methanol and formic acid concentrations ranged from 68 to 427 mg/dL and 37 to 91 mg/dL, respectively, whereas corresponding postmortem methanol and formic acid levels ranged from undetectable to 49 mg/dL and undetectable to 48 mg/dL, respectively. Hospital treatment of formic acid toxicity resulted in significantly reduced postmortem methanol and formic acid concentrations. Furthermore, the toxicological relevance of nine methanol-positive cases where postmortem methanol concentrations ranged from 3 to 142 mg/dL, with corresponding formic acid levels of less than 10 mg/dL, is discussed.
Janus kinase 3 regulates adherens junctions and epithelial mesenchymal transition through β-catenin (2017)

Compromise in adherens junctions (AJs) is associated with several chronic inflammatory diseases. We reported previously that Janus kinase 3, a non-receptor tyrosine kinase, plays a crucial role in AJ formation through its interaction with β-catenin. In this report, we characterize the structural determinants responsible for Jak3 interactions with β-catenin and determine the functional implications of previously unknown tyrosine residues on β-catenin phosphorylated by Jak3. We demonstrate that Jak3 autophosphorylation was the rate-limiting step during Jak3 trans-phosphorylation of β-catenin, where Jak3 directly phosphorylated three tyrosine residues, <i>viz.</i> Tyr<sup>30</sup>, Tyr<sup>64</sup>, and Tyr<sup>86</sup> in the N-terminal domain (NTD) of β-catenin. However, prior phosphorylation of β-catenin at Tyr<sup>654</sup> was essential for further phosphorylation of β-catenin by Jak3. Interaction studies indicated that phosphorylated Jak3 bound to phosphorylated β-catenin with a dissociation constant of 0.28 μm, and although both the kinase and FERM (Band <u>4</u>1, <u>e</u>zrin, <u>r</u>adixin, and <u>m</u>oesin) domains of Jak3 interacted with β-catenin, the NTD domain of β-catenin facilitated its interactions with Jak3. Physiologically, Jak3-mediated phosphorylation of β-catenin suppressed EGF-mediated epithelial-mesenchymal transition and facilitated epithelial barrier functions by AJ localization of phosphorylated β-catenin through its interactions with α-catenin. Moreover, loss of Jak3-mediated phosphorylation sites in β-catenin abrogated its AJ localization and compromised epithelial barrier functions. Thus, we not only characterize Jak3 interaction with β-catenin but also demonstrate the mechanism of molecular interplay between AJ dynamics and EMT by Jak3-mediated NTD phosphorylation of β-catenin.
Monolithic metal-containing TiO2 aerogels assembled from crystalline pre-formed nanoparticles as efficient photocatalysts for H2 generation (2020)

Nanoparticle-based aerogels are 3-dimensional (3D) assemblies of macroscopic size that maintain the intrinsic properties of the initial nanoparticles. Accordingly, they bear immense potential to become an emerging platform for designing new and efficient photocatalysts. However, to take full advantage of this strategy, understanding of the multiscale processes occurring in such 3D-architectures is essential. Here, we prepared aerogels by co-assembling spherical Au, Pd, and PdAu with TiO2 nanoparticles and investigated their photocatalytic properties for hydrogen generation. During gelation, the anatase nanoparticles undergo oriented attachment, homogeneously entrapping the metal nanoparticles in the growing network. The aerogels offer a high porosity with a mean pore size of ca. 34 nm and a large surface area of about 450 m2 g−1. The porous structure enhances the light-harvesting, reagent transport, and electron migration process, generating 3.5-fold more hydrogen in comparison to the corresponding powders.
[Differential gene expression profile in ischemic myocardium of Wistar rats with acute myocardial infarction: the study on gene construction, identification and function]. (2008)

AMI causes a series of changes in gene expression, in which the abnormal expression of genes related to energy metabolism could be one of the molecular mechanisms of AMI. The intervention of the expressions of COX5a and ATP5e may be a new target for AMI therapy.
Optimization and Physicochemical Characterization of Thermosensitive Poloxamer Gel Containing Puerarin for Ophthalmic Use (2006)

The purpose of this study was to systematically optimize an ophthalmic thermosensitive poloxamer analogs gel containing puerarin that was a free flowing liquid below the room temperature and could shift to a gel with an eligible gel strength and bioadhesive force in physiological condition (dilution by the simulated tear fluid and at 35.0 degrees C). A two-factor, five-level central composite design (CCD) was employed to the optimization procedure. The effect of formulation variables (the w/v concentration of poloxamer 407 (X1) and poloxamer 188 (X2)) on a number of response variables (the gelation temperature before (Y1) and after (Y2) the simulated tear fluid diluted, the difference between them (Y3)) was systemically investigated. A second order polynomial equation was fitted to the data. The resulting equation and response surface plots were used to predict the responses in the optimal region. Finally, 21.0% (w/v) poloxamer 407 and 5.0% (w/v) poloxamer188 were chosen as the optimal poloxamer gel matrix. The influence of the other ingredients on the physicochemical properties of the formulation was also investigated. Hydroxypropyl-beta-cyclodextrin (HPCD) enhanced the gelation temperature and reduced the gel strength and the bioadhesive force, while puerarin and benzalkonium chloride (BC) had a comparatively smaller influence. All the isotonicity agents studied had the gelation temperatures lowered, and the gel strengths and the bioadhesive forces enhanced. But only sodium chloride appears to be a promising isotonicity agent for the poloxamer gel containing puerarin, HPCD and BC.
Leak Location in Subsea Umbilicals (2007)

Abstract Leaks in subsea umbilical systems are highly problematic as they are difficult to locate and the eventual egress of fluid may be a considerable distance from the actual line failure due to the presence of additional outer sheath(s). However, in late 2006, by applying their knowledge of how leaks affect the pressure and flow characteristics in a pipeline, Brinker Technology developed an analytical tool which enables them to calculate the approximate location of a leak in a single line of an umbilical bundle. This technique was effectively put to use in November 2006 when, on behalf of Norsk Hydro, Brinker Technology were able to successfully calculate the location of a leak in an Ormen Lange main umbilical. Further to an analytical leak location assessment Brinker can deploy their proven core product, Platelet Technology®, to seal the leak and add further assurance and a greater degree of accuracy to the location estimate. Platelet Technology® involves the remote injection of discrete particles known as "Platelets®" into a pipeline which are carried to the leak site due to the flow. When they reach the vicinity of the leak fluid forces entrain them into the leak and hold them against the pipe wall thus facilitating sealing. By doping the Platelets® with a radioisotope prior to deployment an ROV mounted detection device can then be used in this region to pin-point the exact location of the entrained Platelets® and hence the leak. Both these techniques, the analytical leak location tool and Platelet Technology®, will be presented in this paper with emphasis on the recent operation for Norsk Hydro.
Effect of high stretching rates on the piezoelectric behavior and the structure of polyvinylidene difluoride films (1984)

It has been shown that both the stretch rate and the ambient temperature during stretching affect the ultimate piezoactivity of the PVF2 film. The highest piezoactivity was obtained for films stretched at an 80–90 °C ambient. The piezoactivity went through a minimum at a stretch rate of 1300 mm/min and then increased. This behavior was also observed in structural changes in the films.
Constitutive expression of the granulocyte-macrophage colony-stimulating factor gene in human solid tumors. (1987)

We detected constitutive expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in 3 human solid tumors by Northern blot analysis. Two of them were also found to secrete the GM-CSF protein by colony forming unit-culture assay. Southern blot analysis of each tumor DNA showed no gross rearrangement of the GM-CSF gene. This is the first report that demonstrates expression of the GM-CSF gene in solid tumors at the mRNA level.
Planck2013 results. XXXI. Consistency of thePlanckdata (2014)

The Planck design and scanning strategy provide many levels of redundancy that can be exploited to provide tests of internal consistency. One of the most important is the comparison of the 70 GHz (amplifier) and 100 GHz (bolometer) channels. Based on different instrument technologies, with feeds located differently in the focal plane, analysed independently by different teams using different software, and near the minimum of diffuse foreground emission, these channels are in effect two different experiments. The 143 GHz channel has the lowest noise level on Planck, and is near the minimum of unresolved foreground emission. In this paper, we analyse the level of consistency achieved in the 2013 Planck data. We concentrate on comparisons between the 70, 100, and 143 GHz channel maps and power spectra, particularly over the angular scales of the first and second acoustic peaks, on maps masked for diffuse Galactic emission and for strong unresolved sources. Difference maps covering angular scales from 8° to 15′ are consistent with noise, and show no evidence of cosmic microwave background structure. Including small but important corrections for unresolved-source residuals, we demonstrate agreement (measured by deviation of the ratio from unity) between 70 and 100 GHz power spectra averaged over 70 ≤ ℓ ≤ 390 at the 0.8% level, and agreement between 143 and 100 GHz power spectra of 0.4% over the same ℓ range. These values are within and consistent with the overall uncertainties in calibration given in the Planck 2013 results. We also present results based on the 2013 likelihood analysis showing consistency at the 0.35% between the 100, 143, and 217 GHz power spectra. We analyse calibration procedures and beams to determine what fraction of these differences can be accounted for by known approximations or systematicerrors that could be controlled even better in the future, reducing uncertainties still further. Several possible small improvements are described. Subsequent analysis of the beams quantifies the importance of asymmetry in the near sidelobes, which was not fully accounted for initially, affecting the 70/100 ratio. Correcting for this, the 70, 100, and 143 GHz power spectra agree to 0.4% over the first two acoustic peaks. The likelihood analysis that produced the 2013 cosmological parameters incorporated uncertainties larger than this. We show explicitly that correction of the missing near sidelobe power in the HFI channels would result in shifts in the posterior distributions of parameters of less than 0.3σ except for As, the amplitude of the primordial curvature perturbations at 0.05 Mpc-1, which changes by about 1σ. We extend these comparisons to include the sky maps from the complete nine-year mission of the Wilkinson Microwave Anisotropy Probe (WMAP), and find a roughly 2% difference between the Planck and WMAP power spectra in the region of the first acoustic peak.
Retrovirus‐mediated Gene Transfer Targeted to Malignant Glioma Cells in Murine Brain (1992)

A murine model for meningeal metastasis of malignant glioma was developed to study selective gene transfer into tumor cells and to establish a reliable means of determining the rate of tumor cell infection. A murine ecotropic retroviral vector was created in which the Escherichia coli beta-galactosidase gene served as a marker for gene expression from the integrated retrovirus. This retrovirus exhibited a high rate of infectivity in RSV-M mouse glioma cells in vitro. The recombinant retrovirus was injected directly into the cisterna magna of the mice. Staining of beta-galactosidase showed that the rate of gene integration was high in the disseminated glioma cells. These results suggest the possibility of retrovirus-mediated gene therapy for meningeal dissemination of malignant glioma.
Nontypeable Haemophilus influenzae and childhood pneumonia. (2010)

Nontypeable Haemophilus influenzae (NTHi) is a common microbe frequently isolated from the nasopharynx of children. Bacterial pneumonia is a major cause of morbidity and mortality in children less than 5 years of age, with the burden of disease being greatest in developing countries. Determination of the bacterial aetiology of pneumonia is difficult due to sampling constraints. However, with a combination of sampling approaches, trans-thoracic fine-needle aspiration, blood culture and screened sputum, the evidence strongly suggests that NTHi is a significant causative pathogen of pneumonia in young children. However, further studies are required. The development of a new pneumococcal conjugate vaccine containing H. influenzae protein D has the potential to be beneficial against disease caused by NTHi, including pneumonia. With the implementation of this vaccine in many regions of the world where NTHi disease is endemic, it will be critical to introduce surveillance programs wherever it is used.
Characterization of fluid flow using low‐spatial‐resolution velocity spectra from NMR images (1989)

Flow velocity distributions or spectra may be obtained by NMR imaging using an anisotropic three-dimensional Fourier transform pulse sequence in which the low-resolution direction encodes perpendicular velocity. Velocity spectra from regions of interest covering the entire lumens of pipes in a flow phantom containing straight sections, a jet, and a constriction suggest that velocity spectra provide useful information even when the vessels are not spatially well-resolved. The flow in the phantom was characterized and limited measurements were made.
A Noise-Reduction Program in a Pediatric Operation Theatre Is Associated With Surgeon's Benefits and a Reduced Rate of Complications (2013)

Spontaneous noise during pediatric operations attains the magnitude of a lawn mower and peaks resemble a passing truck. The sound intensity could be reduced by 50% by specific measures. This reduction was associated with a significantly lowered number of postoperative complications. The surgeon's benefits are idiosyncratic with "responders" experiencing marked improvements.
Gettering of Au to dislocations and cavities in silicon (1995)

The gettering of ion implanted Au to defects in Si has been studied using Rutherford backscattering and channeling and transmission electron microscopy. Damage from a Si implant anneals into dislocations which can efficiently trap diffusing Au. The damage introduced by a H implant evolves during annealing into cavities which getter close to 100% of the Au, leaving very little Au in solution. This process is driven by the diffusion of a supersaturated solid solution of Au to a favorable sink. The internal surfaces of cavities are the most favorable sink, followed by dislocations and then the Si surface.
Threshold Voltage Tuning for Faster Activation With Lower Noise in Tri-Mode MTCMOS Circuits (2011)

A new threshold voltage tuning methodology is explored in this paper to minimize the peak power/ground bouncing noise with smaller sleep transistors in multi-threshold CMOS (MTCMOS) circuits. Different circuit techniques with the threshold voltage tuning strategy lower the activation noise, the activation delay, and the size of the additional sleep transistors by up to 27.76%, 32.66%, and 85.71%, respectively, as compared to a previously published noise-aware MTCMOS circuit with standard zero-body-biased high threshold voltage sleep transistors in a UMC 80-nm CMOS technology.