gebhart/patrepeval huggingface dataset:
Sequence of Two Plasmids from Clostridium perfringens Chicken Necrotic Enteritis Isolates and Comparison with C. perfringens Conjugative Plasmids (2012)

Twenty-six isolates of Clostridium perfringens of different MLST types from chickens with necrotic enteritis (NE) (15 netB-positive) or from healthy chickens (6 netB-positive, 5 netB-negative) were found to contain 1-4 large plasmids, with most netB-positive isolates containing 3 large and variably sized plasmids which were more numerous and larger than plasmids in netB-negative isolates. NetB and cpb2 were found on different plasmids consistent with previous studies. The pathogenicity locus NELoc1, which includes netB, was largely conserved in these plasmids whereas NeLoc3, present in the cpb2 containing plasmids, was less well conserved. A netB-positive and a cpb2-positive plasmid were likely to be conjugative, and the plasmids were completely sequenced. Both plasmids possessed the intact tcp conjugative region characteristic of C. perfringens conjugative plasmids. Comparative genomic analysis of nine CpCPs, including the two plasmids described here, showed extensive gene rearrangements including pathogenicity locus and accessory gene insertions around rather than within the backbone region. The pattern that emerges from this analysis is that the major toxin-containing regions of the variety of virulence-associated CpCPs are organized as complex pathogenicity loci. How these different but related CpCPs can co-exist in the same host has been an unanswered question. Analysis of the replication-partition region of these plasmids suggests that this region controls plasmid incompatibility, and that CpCPs can be grouped into at least four incompatibility groups.
3-dimensional nano-CMOS transistors to overcome scaling limits (2004)

Among the several approaches to overcome the scaling limit of silicon devices, in this paper we introduce recent achievements in the fabrication of transistors for SRAM, DRAM and Flash memories as well as the logic devices with high performance requirement. Among them are S3 (stacked single-crystal silicon) cell for SRAMs, RCAT (recessed cell array transistor), PiFET (partially-insulated MOSFET) and FinFET for DRAMs and MBCFET (multi-bridge channel MOSFET) for high performance logic applications. These 3 dimensional devices are easily adapted to the products showing excellent performance.
Spectral Methods for the Estimation of Acoustic Intensity, Energy Density, and Surface Velocity Using a Multimicrophone Probe (1984)

This reproduction was made from a copy of a document sent to us for microfilming.While the most advanced technology has been used to photograph and reproduce this document, the quality of the reproduction is heavily dependent upon the quality of the material submitted.The following explanation of techniques is provided to help clarify markings or notations which may appear on this reproduction.1.The sign or "target" for pages apparently lacking from the document photographed is "Missing Page(s)".I f it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages.This may have necessitated cutting through an image and duplicating adjacent pages to assure complete continuity.2. When an image on the film is obliterated with a round black mark, it is an indication of either blurred copy because of movement during exposure, duplicate copy, or copyrighted materials that should not have been filmed.For blurred pages, a good image of the page can be found in the adjacent frame.If copyrighted materials were deleted, a target note will appear listing the pages in the adjacent frame.3. When a map, drawing or chart, etc., is part of the material being photographed, a definite method of "sectioning" the material has been followed.It is customary to begin filming at the upper left hand comer of a large sheet and to continue from left to right in equal sections with small overlaps.I f necessary, sectioning is continued again-beginning below the first row and continuing on until complete. 4.
Correction to: Towards Quantitatively Understanding the Complexity of Social-Ecological Systems—From Connection to Consilience (2017)

The original publication was published without Acknowledgments section. The missing section is printed here.
Effects of Some Rare Elements on Nicotine Content of the Tobacco Plant (1973)

Fifty-four rare elements were tested for their effects on the nicotine level of tobacco (Nicotiana tabacum L.) plants grown in solution culture. Be, Cu, Pd, Pt, and Sm definitely increased nicotine yield (over 25%), whereas Bi, Co, Ho, Pb, Ni, Rb, Ag, Tl, Sn, U. V. and Zr definitely decreased nicotine yield. Cs, Er, Li, Rh, Ru, Se, Sr, Ti, and Yb possibly increased (less than 25%) nicotine yield, whereas As, Ce, Cr, Dy, Gd, I, Mo, Nd, Re, Ta, and Th possibly decreased nicotine yield. Other elements including Al, Ge, Au, Hf, In, Ir, La, Lu, Hg, Os, Pr, Sc, Te, Tb, Tm, W, and Zn showed no significant effects.
Coexpression of FOXP3 and a Helios isoform enhances the effectiveness of human engineered regulatory T cells (2020)

Abstract Regulatory T cells (Tregs) are a subset of immune cells that suppress the immune response. Treg therapy for inflammatory diseases is being tested in the clinic, with moderate success. However, it is difficult to isolate and expand Tregs to sufficient numbers. Engineered Tregs (eTregs) can be generated in larger quantities by genetically manipulating conventional T cells to express FOXP3. These eTregs can suppress in vitro and in vivo but not as effectively as endogenous Tregs. We hypothesized that ectopic expression of the transcription factor Helios along with FOXP3 is required for optimal eTreg immunosuppression. To test this theory, we generated eTregs by retrovirally transducing total human T cells (CD4+ and CD8+) with FOXP3 alone or with each of the 2 predominant isoforms of Helios. Expression of both FOXP3 and the full-length isoform of Helios was required for eTreg-mediated disease delay in a xenogeneic graft-versus-host disease model. In vitro, this corresponded with superior suppressive function of FOXP3 and full-length Helios-expressing CD4+ and CD8+ eTregs. RNA sequencing showed that the addition of full-length Helios changed gene expression in cellular pathways and the Treg signature compared with FOXP3 alone or the other major Helios isoform. Together, these results show that functional human CD4+ and CD8+ eTregs can be generated from total human T cells by coexpressing FOXP3 and full-length Helios.
Development of a Flexible and Robust Synthesis of Tetrahydrofuro[3,4-b]furan Nucleoside Analogues (2021)

In the context of a PRMT5 inhibitor program, we describe our efforts to develop a flexible and robust strategy to access tetrahydrofuro[3,4-<i>b</i>]furan nucleoside analogues. Ultimately, it was found that a Wolfe type carboetherification from an alkenol derived from d-glucofuranose diacetonide was capable of furnishing the B-ring and installing the desired heteroaryl group in a single step. Using this approach, key intermediate <b>1.3-A</b> was delivered on a gram scale in a 62% yield and 9.1:1 dr in favor of the desired <i>S</i>-isomer. After deprotection of <b>1.3-A</b>, a late-stage glycosylation was performed under Mitsunobu conditions to install the pyrrolopyrimidine base. This provided serviceable yields of nucleoside analogues in the range of 31-48% yield. Compound <b>1.1-C</b> was profiled in biochemical and cellular assays and was demonstrated to be a potent and cellularly active PRMT5 inhibitor, with a PRMT5-MEP50 biochemical IC<sub>50</sub> of 0.8 nM, a MCF-7 target engagement EC<sub>50</sub> of 3 nM, and a Z138 cell proliferation EC<sub>50</sub> of 15 nM. This work sets the stage for the development of new inhibitors of PRMT5 and novel nucleoside chemical matter for alternate drug discovery programs.
ABC Transporters in the Development of Multidrug Resistance in Cancer Therapy (2016)

Cancer is one of the leading causes of mortality in the developed countries. The search for novel drug candidates is a priority goal for cancer therapy. However, the emergence of drug resistance reduces the effectiveness of new medications. The ATP binding cassette (ABC) family of proteins are efflux pumps that transport various structurally unrelated and potentially dangerous substances out of the cells. These transporters have evolved as a complex cellular defense system, for the recognition and removal of toxic agents entering the cells from their environment. Several of the ABC transporters are related to chemoresistance in cancer therapy, as the rapidly dividing malignant cells use them to protect themselves from medical interventions. Here we review the members of the ABC transporter families responsible for the multidrug resistance in anticancer therapy.
Effects of a novel 2,3-oxidosqualene cyclase inhibitor on the regulation of cholesterol biosynthesis in HepG2 cells. (1996)

Within the cholesterol biosynthesis cascade, the enzyme 2,3-oxidosqualene cyclase [EC 5.4.99.7] is of special interest due to its dual function: cyclization of 2,3-monoepoxysqualene to lanosterol and 2,3;22,23-diepoxysqualene to oxylanosterol. Further determination of the significance of this enzyme for the intracellular cholesterol homeostasis was done with BIBX 79, a new potent, specific inhibitor of this enzyme. In HepG2 cells the effects of BIBX 79 on cholesterol biosynthesis, 2,3-oxidosqualene cyclase as well as HMG-CoA reductase activities were studied. BIBX 79 is a potent inhibitor of sterol biosynthesis in HepG2 cells (IC50 4 x 10(-9) M). No other enzyme within the cholesterol biosynthesis cascade was significantly inhibited as was evidenced by a radio HPLC detection system. In contrast to simvastatin, no direct interaction with HMG-CoA reductase was observed. When incubating HepG2 cells for 16 h with the HMG-CoA reductase inhibitor simvastatin (10(-6)-10(-10) M) HMG-CoA reductase activity was increased up to 180%. BIBX 79 did also affect HMG-CoA reductase activity under these conditions: in concentrations of BIBX 79 "> or =" 10(-9) "< or =" 10(-7) M, where a partial inhibition of 2,3-oxidosqualene cyclase is observed, HMG-CoA reductase activity was decreased. However, higher concentrations of BIBX 79 that totally blocked 2,3-oxidosqualene cyclase led to an increase in HMG-CoA reductase activity. This effect of BIBX 79 on HMG-CoA reductase is thought to be mainly mediated by oxysterols that are formed by the cyclization of 2,3;22,23-diepoxysqualene. 2,3;22,23-Diepoxysqualene is preferentially cyclized by the 2,3-oxidosqualene cyclase and, consequently, only high inhibitor concentrations will also block 2,3;22,23-diepoxysqualene cyclization. Thus, by partial blockade of this enzyme, both an inhibition of lanosterol and subsequently cholesterol formation as well as a concomitant effect on HMG-CoA reductase can be achieved. Both effects complement each other and lead to an effective control of cholesterol biosynthesis. It is therefore concluded that 2,3-oxidosqualene cyclase plays a crucial role in the regulation of intracellular cholesterol homeostasis. 2,3-Oxidosqualene cyclase inhibitors offer an attractive approach for novel lipid-lowering agents.
ChemInform Abstract: SYNTHESIS AND ANTIVIRAL ACTIVITY OF CERTAIN 5′‐MONOPHOSPHATES OF 9‐ARABINOFURANOSYLHYPOXANTHINE AND 9‐D‐ARABINOFURANOSYLHYPOXANTHINE (1975)

Abstract Aus Ara‐A (I) erhält man bei der Phosphorylierung die Monophosphate (IIa) und (IIb), die mit Nitrit zu (IIIa) und (IIIb) desaminiert werden können.
Prehospital monitoring of cerebral circulation during out of hospital cardiac arrest ? A feasibility study (2022)

Our results suggest that both INVOS™5100 and ROOT® O3 can distinguish between ROSC and no ROSC in OHCA, and both could be used in the pre-hospital setting and during transport with minimal interference with ALS.
Cerium Oxide Nanoclusters on Graphene/Ru(0001): Intercalation of Oxygen via Spillover (2015)

Cerium oxide is an important catalytic material known for its ability to store and release oxygen, and as such, it has been used in a range of applications, both as an active catalyst and as a catalyst support. Using scanning tunneling microscopy and Auger electron spectroscopy, we investigated oxygen interactions with CeOx nanoclusters on a complete graphene monolayer-covered Ru(0001) surface at elevated temperatures (600-725 K). Under oxidizing conditions (PO2 = 1 × 10(-7) Torr), oxygen intercalation under the graphene layer is observed. Time dependent studies demonstrate that the intercalation proceeds via spillover of oxygen from CeOx nanoclusters through the graphene (Gr) layer onto the Ru(0001) substrate and extends until the Gr layer is completely intercalated. Atomically resolved images further show that oxygen forms a p(2 × 1) structure underneath the Gr monolayer. Temperature dependent studies yield an apparent kinetic barrier for the intercalation of 1.21 eV. This value correlates well with the theoretically determined value for the reduction of small CeO2 clusters reported previously. At higher temperatures, the intercalation is followed by a slower etching of the intercalated graphene (apparent barrier of 1.60 eV). Vacuum annealing of the intercalated Gr leads to the formation of carbon monoxide, causing etching of the graphene film, demonstrating that the spillover of oxygen is not reversible. In agreement with previous studies, no intercalation is observed on a complete graphene monolayer without CeOx clusters, even in the presence of a large number of point defects. These studies demonstrate that the easily reducible CeOx clusters act as intercalation gateways capable of efficiently delivering oxygen underneath the graphene layer.
Tensor Fusion Network for Multimodal Sentiment Analysis (2017)

Multimodal sentiment analysis is an increasingly popular research area, which extends the conventional language-based definition of sentiment analysis to a multimodal setup where other relevant modalities accompany language. In this paper, we pose the problem of multimodal sentiment analysis as modeling intra-modality and inter-modality dynamics. We introduce a novel model, termed Tensor Fusion Network, which learns both such dynamics end-to-end. The proposed approach is tailored for the volatile nature of spoken language in online videos as well as accompanying gestures and voice. In the experiments, our model outperforms state-ofthe-art approaches for both multimodal and unimodal sentiment analysis.
Preimplantation genetic diagnosis in an HIV serodiscordant couple carrier for sickle cell disease: a case report (2008)

info:eu-repo/semantics/nonPublished
Marinobufagenin causes endothelial cell monolayer hyperpermeability by altering apoptotic signaling (2009)

Marinobufagenin (MBG) is an endogenous mammalian cardiotonic steroid that is involved in the inhibition of the sodium pump Na(+)/K(+)-ATPase. Increased plasma levels of MBG have been reported in patients with preeclampsia. MBG increases microvascular barrier permeability in an animal model of preeclampsia. However, the mechanism by which MBG impairs endothelial permeability is unknown. We utilized rat lung microvascular endothelial cells (RLMEC) to examine alterations in MBG-induced monolayer permeability and the effect of MBG on the phosphorylation status of ERK1/2, Jnk, and p38. Apoptosis was evaluated by examining alterations in caspases 3/7, 8, and 9 and annexin-V staining. We also examined the effect of MBG on the endothelial adherens junctions of the RLMEC monolayer. MBG inhibited the proliferation, and increased the monolayer permeability, of RLMEC. These actions of MBG were attenuated by ERK, p38, and pan caspase inhibition. MBG significantly decreased the phosphorylation of ERK1/2 and activated the phosphorylation of Jnk and p38. MBG also significantly increased the expression of caspases 3/7, 8, and 9, indicating the activation of apoptosis. MBG-induced apoptosis signaling was not observed in cells pretreated with a p38 inhibitor. MBG treatment induced the disruption of endothelial cell junctions. This effect was prevented by a pan caspase inhibitor. In conclusion, 1) MBG induced an impairment of RLMEC proliferation; 2) the bufadienolide also caused endothelial hyperpermeability; and 3) these effects of MBG were mediated by the downregulation of ERK1/2, the upregulation of Jnk and p38, by the activation of apoptosis, and by the disruption of endothelial cell junctions.
Sizable room-temperature magnetoresistance in cobalt based magnetic tunnel junctions with out-of-plane anisotropy (2008)

Submicron alumina based magnetic tunnel junctions (MTJs) using electrodes with out-of-plane magnetic anisotropy were prepared and characterized. Both electrodes are industry-compatible Co∕Pt multilayers. The magnetic properties of the unpatterned samples have been investigated through superconducting quantum interference device (SQUID) magnetometry and extraordinary Hall effect: both electrodes have fully out-of-plane magnetic moments and nonoverlapping coercive fields. Transport measurements on the submicron MTJs showed a magnetoresistance (MR) ratio reaching 8% at room temperature. Nanopillars with diameters of 800, 400, and 200nm patterned from the same wafer show the expected out-of-plane magnetic properties and similar resistance×area products (RA) and MR ratios. The I(V) characteristics of pillars with diameters of 800 and 400nm could be accounted for with reasonable barrier heights and widths.
[The care of the sexuality among oncologic patients - A research project within the University hospitals of Geneva]. (2018)

Cancer affects sexual function, sexual health and relationships as well as mental health and quality of life. Due to the importance of sexual health for each individual and in order to ensure optimal and quality care, we have assessed the demand for sexual health and advise not only among cancer patients but also among professionals in this area and nursing staff. The results showed that sexuality was important both for patients and caregivers. Also we did find out that carers want to create a better professional network with the aim of redirecting patients to specialists according to their specific needs. Carers have also expressed a desire to have relevant training in sexology and sexual health to achieve this.
Production potential of guava-based hortipastoral system with different In-situ mois ture conservation techniques (2017)

A field experiment (2007–2016) was conducted to evaluate the performance of guava based hortipasture system in under rainfed situation in semi-arid condition. The experiment consisted 12 treatments i.e. 2 cultivars (Shweta and Lalit) with pasture (Cenchrus ciliaris + Stylosanthes hamata) under 6-treatment viz., vegetative barrier, staggered trenches (2m x 0.50m x 0.50m), stone mulch in basin (locally available stone in 50 cm radius), guava + pasture (without soil and water conservation), sole pasture and sole guava. The cultivar Lalit produced better yield (20 percent higher) as compared to Cv. Shweta. Over the nine years the average yield of pasture production with staggered trenches was higher (7.0 t DM/ha) followed by vegetative barriers (6.4 t DM/ha) as compared to control (T4). The minimum pasture production (5.3 t DM/ha) was recorded with stone mulch in basin. The pasture production in association with tree was little higher (6.1 t DM/ha) as compared to sole pasture (6.0 t DM/ha).
Inhibitors of appressorium formation inMagnaporthe grisea: a new approach to control rice blast disease (1998)

Fungi were screened for the production of inhibitors of appressorium formation in germinating conidiospores of Magnaporthe grisea on inductive and non-inductive surfaces. Bioactivity-guided isolation yielded glisoprenins A, C, D and E from Gliocladium roseum and oleic acid from three fungi. The glisoprenins were active only on a hydrophobic surface, whereas oleic acid inhibited appressorium formation on a hydrophilic surface when 1,16-hexadecanediol, but not 8-(4-chlorophenylthio)adenosine-3′,5′-monophosphate, was used as inducer. The inhibition by glisoprenins could be reversed competitively by 1,2-dioctanoylglycerol but not by 1-oleoyl-2-acetyl-glycerol, both effective activators of protein kinase C in mammalian cells. Other mono-unsaturated fatty acids also inhibited appressorium formation. The corresponding methyl esters were inactive. The results agree with previous findings that at least two signal-transducing pathways are involved in appressorium formation. In addition, the differences observed between fungal signalling via PKC and the pathway used in mammalian cells could be used for the search for new and selective fungicides for rice blast disease. © 1998 Society of Chemical Industry
Evaluation of Three Live Attenuated H2 Pandemic Influenza Vaccine Candidates in Mice and Ferrets (2013)

Influenza pandemics arise when novel influenza viruses are introduced into a population with little prior immunity to the new virus and often result in higher rates of illness and death than annual seasonal influenza epidemics. An influenza H2 subtype virus caused a pandemic in 1957, and H2 viruses circulated in humans till 1968. H2 influenza viruses continue to circulate in birds, and the development of an H2 influenza vaccine candidate is therefore considered a priority in preparing for future pandemics. However, we cannot predict whether a human H2 virus will reemerge or a novel avian H2 virus will emerge. We identified three viruses as suitable candidates for further evaluation as vaccines to protect against H2 influenza viruses and evaluated the immune responses and protection that these three vaccines provided in mice and ferrets.
Chromosomal breaks at the origin of small tandem DNA duplications (2022)

Abstract Small tandem DNA duplications in the range of 15 to 300 base‐pairs play an important role in the aetiology of human disease and contribute to genome diversity. Here, we discuss different proposed mechanisms for their occurrence and argue that this type of structural variation mainly results from mutagenic repair of chromosomal breaks. This hypothesis is supported by both bioinformatical analysis of insertions occurring in the genome of different species and disease alleles, as well as by CRISPR/Cas9‐based experimental data from different model systems. Recent work points to fill‐in synthesis at double‐stranded DNA breaks with complementary sequences, regulated by end‐joining mechanisms, to account for small tandem duplications. We will review the prevalence of small tandem duplications in the population, and we will speculate on the potential sources of DNA damage that could give rise to this mutational signature. With the development of novel algorithms to analyse sequencing data, small tandem duplications are now more frequently detected in the human genome and identified as oncogenic gain‐of‐function mutations. Understanding their origin could lead to optimized treatment regimens to prevent therapy‐induced activation of oncogenes and might expose novel vulnerabilities in cancer.
Time-Resolved Luminescence Characteristics of Cerium Doped YAG Nanocrystals (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.