gebhart/patrepeval huggingface dataset:
Radiation exposure during the implantation of bioabsorbable vascular scaffolds versus drug-eluting stents in non-complex coronary lesions: a matched-cohort study (2016)

The implantation of BVS in combination with a simple four-step protocol is a feasible option for interventional treatment of non-complex coronary lesions without significant impact on radiation exposure or outcome measures in daily clinical routine.
Mapping the Shapes of Phylogenetic Trees from Human and Zoonotic RNA Viruses (2013)

A phylogeny is a tree-based model of common ancestry that is an indispensable tool for studying biological variation. Phylogenies play a special role in the study of rapidly evolving populations such as viruses, where the proliferation of lineages is constantly being shaped by the mode of virus transmission, by adaptation to immune systems, and by patterns of human migration and contact. These processes may leave an imprint on the shapes of virus phylogenies that can be extracted for comparative study; however, tree shapes are intrinsically difficult to quantify. Here we present a comprehensive study of phylogenies reconstructed from 38 different RNA viruses from 12 taxonomic families that are associated with human pathologies. To accomplish this, we have developed a new procedure for studying phylogenetic tree shapes based on the 'kernel trick', a technique that maps complex objects into a statistically convenient space. We show that our kernel method outperforms nine different tree balance statistics at correctly classifying phylogenies that were simulated under different evolutionary scenarios. Using the kernel method, we observe patterns in the distribution of RNA virus phylogenies in this space that reflect modes of transmission and pathogenesis. For example, viruses that can establish persistent chronic infections (such as HIV and hepatitis C virus) form a distinct cluster. Although the visibly 'star-like' shape characteristic of trees from these viruses has been well-documented, we show that established methods for quantifying tree shape fail to distinguish these trees from those of other viruses. The kernel approach presented here potentially represents an important new tool for characterizing the evolution and epidemiology of RNA viruses.
Bistable springs for wideband microelectromechanical energy harvesters (2013)

This paper presents experimental results on a microelectromechanical energy harvester with curved springs that demonstrates an extremely wide bandwidth. The springs display an asymmetrical bistable behavior obtained purely through their geometrical design. The frequency down-sweep shows that the harvester 3-dB bandwidth is about 587 Hz at 0.208-g acceleration amplitude. For white noise excitation at 4×10−3 g2/Hz, we found that the bandwidth reaches 715 Hz, which is more than 250 times wider than in the linear-spring regime. By varying the bias voltage, an output power of 3.4 μW is obtained for frequency down-sweep at 1-g amplitude and 150-V bias.
Improvement of Crystal Solubility and Increasing Toxicity against Caenorhabditis elegans by Asparagine Substitution in Block 3 of Bacillus thuringiensis Crystal Protein Cry5Ba (2012)

The crystal proteins from Bacillus thuringiensis are widely used for their specific toxicity against insects and nematodes. The highly conserved sequence blocks play an important role in Cry protein stability and flexibility, the basis of toxicity. The block 3 in Cry5Ba subfamily has a shorter sequence (only 12 residues) and more asparagine residues than that of others which harbor about 48 residues but only one asparagine. Based on the theoretical structure model of Cry5Ba, all three asparagines in block 3 are closely located in the interface of putative three domains, implying their probable importance in structure and function. In this study, all three asparagines in Cry5Ba2 block 3 were individually substituted with alanine by site-directed mutagenesis. The wild-type and mutant proteins were overexpressed and crystallized in acrystalliferous B. thuringiensis strain BMB171. However, the crystals formed in one of the mutants, designated N586A, abnormally disappeared and dissolved into the culture supernatant once the sporulation cells lysed, whereas the Cry5Ba crystal and the other mutant crystals were stable. The mutant N586A crystal, isolated from sporulation cells by the ultrasonic process, was found to be easily dissolved at wide range of pH value (5.0 to 10.0). Moreover, the toxicity assays showed that the mutant N586A exhibited nearly 9-fold-higher activity against nematodes and damaged the host's intestine more efficiently than the native Cry5Ba2. These data support the presumption that the amide residue Asn586 at the interface of domains might adversely affect the protein flexibility, solubility and resultant toxicity of Cry5Ba.
Formulation of Sustained Release Hydrophilic Matrix Tablets of Tolcapone with the Application of Sedem Diagram: Influence of Tolcapone’s Particle Size on Sustained Release (2020)

Hydrophilic matrix tablets are a type of sustained release dosage form characterized by distributing a drug in a matrix that is usually polymeric. Tolcapone is a drug that inhibits the enzyme catechol-<i>O</i>-methyl transferase. In recent years, it has been shown that tolcapone is a potent inhibitor of the amyloid aggregation process of the transthyretin protein, and acts by stabilizing the structure of the protein, reducing the progression of familial amyloid polyneuropathy. The main objective of this study was to obtain a sustained release tablet of tolcapone for oral administration with a preferred dosage regimen of 1 administration every 12 or 24 h and manufactured, preferably, by direct compression. The SeDeM Diagram method has been used for the formulation development of hydrophilic matrix tablets. Given the characteristics of tolcapone, the excipient selected for the formation of the polymeric matrix was a high viscosity hydroxypropylmethylcellulose (Methocel<sup>®</sup> K100M CR). A decrease in the particle size of tolcapone resulted in a slower dissolution release of the formulation when the concentration of the polymer Methocel<sup>®</sup> K100M CR was below 29%. These surprising and novel results have given rise to patent number WO/2018/019997.
Increased Susceptibility For Csa-Induced Hepatotoxicity In Kidney Graft RecipieNTSF WITH CHRONIC VIRAL HEAPATITIS C (1993)

CsA-induced hepatotoxicity is a rare disorder in renal transplant recipients when low doses are administered and whole blood trough levels of CsA are regularly monitored. However, there is controversy about the clinical value of measuring CsA-metabolites, whose contribution to immunosuppression and toxicity is not fully understood. To assess the relation between low-dose CsA therapy and hepatotoxicity, we studied 128 renal transplant recipients attending our nephrology clinic. Eight of these patients had markedly elevated liver function tests. Three patients while receiving very low doses of oral CsA (< 3.8 mg/kg of body weight) presented marked derangements of CsA metabolism with abnormally increased CsA-metabolite levels. Parent drug levels were in the normal range. All 3 patients had chronic infection with hepatitis C virus and revealed histomorphologic evidence of hepatotoxicity. Hepatic dysfunction normalized when CsA was withdrawn or reduced by 50%. It is likely that hepatitis C virus infection interferes with CsA metabolism and/or biliary CsA-excretion and thus is responsible for CsA and/or metabolite-induced hepatotoxicity despite very low doses of CsA.
Tracking defectivity of EUV and SADP processing using bright-field inspection (2014)

The purpose of this study is to understand EUV+SADP defectivity in 15nm line and space (L&amp;S) pattern, and to examine bright-field inspection capabilities at the 1Xnm node. Programmed defects of known size, shape, and location were printed in dense patterned areas using EUV lithography at IMEC. To track these defects throughout development, a defectivity study was conducted using bright-field inspection after four SADP processing steps. The smallest defect routinely detected had a programmed size of 14nm, and the defect signal was enhanced using polarized light. By comparing defect locations at the beginning and end stages of development, it was found that 95% of defects remained the same. This illustrates the importance of post-lithography wafer inspection. This research shows how defect characteristics on the EUV mask affect the final pattern and demonstrate the sensitivity of bright-field inspection at the 1Xnm node.
From the ICU to the operating room: how to manage the patient? (2019)

ICU patients who require surgery may benefit from appropriate perioperative management.
A cell‐based location‐sensing method for wireless networks (2003)

Abstract One of the most important applications for mobile commerce is location‐based application and the core technology of location‐based applications is the location‐determination technology. In this paper, we present a location‐sensing method, called cell‐based location‐sensing method and its positioning accuracy for the wireless networks with hexagonal structure and mesh structure. In addition, the accuracy of the method is optimized by tuning the transmitting power of base stations. In the optimal transmitting power, an accuracy of within 9.1667% (7.2182%) cell area for hexagonal structure (mesh structure) can be achieved by the cell‐based location‐sensing method. We believe that the results are useful for deploying wireless networks for location‐based applications. Copyright © 2003 John Wiley &amp; Sons, Ltd.
Changing the Face of Multicultural Counselling with Principles of Change (2001)

Discussion in multicultural counselling has focused on whether the culture specific (emic) or universal position (etic) is more useful. More recently a common factors approach has been introduced. This article provides a critical analysis of each of these positions and introduces the principles of change approach as a way of unifying multicultural counselling and moving the conversation to a more applied level. Directions for future research are provided.
The results of identification of bacteria from positive blood cultures using MALDI-TOF mass spectrometry (2018)

Objective. To evaluate the results of accelerated identification of microorganisms isolated from positive hemocultures obtained from patients of various departments of a large multi-profile hospital by MALDI-TOF MS and to compare them with classical cultural methods. Materials and Methods. In the period from 2017 to 2018, the study included 109 positive blood cultures from 73 patients from UIA GKB number 40 at the age of 1 to 83 years. Blood samples were taken in aseptic conditions from 2 peripheral veins according to the standard protocol in 12 departments of the health facility.
Survey of bacteria associated with western corn rootworm life stages reveals no difference between insects reared in different soils (2018)

Abstract Western corn rootworm ( Diabrotica virgifera virgifera LeConte) is a serious pest of maize ( Zea mays L.) in North America and parts of Europe. With most of its life cycle spent in the soil feeding on maize root tissues, this insect is likely to encounter and interact with a wide range of soil and rhizosphere microbes. Our knowledge of the role of microbes in pest management and plant health remains incomplete. An important component of an effective pest management strategy is to know which microorganisms are present that could play a role in life history or management. For this study, insects were reared in soils from different locations. Insects were sampled at each life stage to determine the possible core bacteriome. Additionally, soil was sampled at each life stage and resulting bacteria were identified to determine the contribution of soil to the rootworm bacteriome, if any. We analyzed the V4 hypervariable region of bacterial 16S rRNA genes with Illumina MiSeq to survey the different species of bacteria associated with the insects and the soils. The bacterial community associated with insects was significantly different from that in the soil. Some differences appear to exist between insects from non-diapausing and diapausing colonies while no significant differences in community composition existed between the insects reared on different soils. Despite differences in the bacteria present in immature stages and in male and female adults, there is a possible core bacteriome of approximately 16 operational taxonomic units ( i . e ., present across all life stages). This research may give insights into how resistance to Bt develops, improved nutrition in artificial rearing systems, and new management strategies.
New aryl Schiff bases of thiadiazole derivative of ibuprofen as DNA binders and potential anticancer drug candidates (2020)

The work presented in this paper describes the synthesis of two new aryl Schiff bases [(<i>E</i>)-<i>N</i>-(4-(benzyloxy)-3-methoxybenzylidene)-5-(1-(4-isobutylphenyl)ethyl)-1,3,4-thiadiazol-2-amine] (ASB-1) and [(<i>E</i>)-<i>N</i>-(4-(benzyloxy)benzylidene)-5-(1-(4-isobutylphenyl)ethyl)-1,3,4-thiadiazol-2-amine] (ASB-2). These compounds were characterized by different analytical techniques and then studied for DNA binding. Binding studies were carried out at neutral pH (7.0) and at 37 °C by theoretical and experimental methods including DFT, molecular docking, spectroscopy (UV-visible, fluorescence), cyclic voltammetry (CV) and viscometry. Further investigations of these compounds were done on hepatocellular carcinoma; Huh-7 cancer cell line. Binding constant, free energy change and binding site size, i.e. K<sub>b</sub>, ΔG and <i>n</i> were evaluated which indicated that both ASB-1 and ASB-2 bind significantly and spontaneously with the DNA. However, data revealed relatively greater binding of ASB-1 with DNA. Spectral and voltammetric results were found supportive of each other. Binding site sizes and viscosity measurements verified the mixed binding mode of interactions as observed in molecular docking analysis, i.e. intercalation with groove binding. DNA binding studies were very well correlated with the <i>in-vitro</i> studies performed on Huh-7 cell line as well as normal HEK-293 cell lines. The compound ASB-1 not only showed greater binding affinity toward DNA but also showed greater anticancer potency with least IC<sub>50</sub> value as compared to ASB-2.
SketchSpace (2011)

This paper presents SketchSpace, a system that allows designers to interactively sketch [3] device's interactive behaviors by imbuing digital functionality to passive materials. SketchSpace requires no augmentation of the device itself, but instead it uses a depth-sensing Kinect camera to simulate the use of hardware sensors by using depth information to infer an object's three-dimensional position, motion, proximity, shape, deformations, and touch events on its surface. A designer can map these inputs to desktop applications in real-time and thus experiment with different interactions. We showcase how SketchSpace can be used to prototype two devices: from tilt sensitive mice to bendable displays. In general, we discuss how this simplifies the process of generating an interactive device sketch and supports rapid exploration of design solutions.
Synergistic interaction of lovastatin and paclitaxel in human cancer cells. (2001)

The hydroxymethylglutaryl-CoA reductase inhibitor lovastatin is used widely to treat hypercholesterolemia and has been shown to have cell cycle-specific effects. In these studies, we have examined the effects of combining lovastatin and paclitaxel (Taxol), a microtubule-stabilizing agent, in the human leukemia K562 and HL-60 cell lines. Isobologram analysis of cytotoxicity assays revealed that there is a synergistic interaction between the two agents in both cell lines. Cell cycle analyses showed that lovastatin enhances paclitaxel-induced G2-M arrest in both cell lines. In addition, Annexin V apoptotic studies revealed that lovastatin enhances paclitaxel-induced apoptosis in HL-60 cells. Lovastatin did not affect levels of [3H]paclitaxel in cells. Whereas lovastatin induced an accumulation of unmodified Ras and caused an up-regulation of both RhoB and Rap1A, paclitaxel was found to have no effect on the isoprenylated proteins. Studies of the centromere-associated protein mitosin revealed that treatment with lovastatin and paclitaxel resulted in increased mitosin levels and that lovastatin altered the association of mitosin with condensed chromosomes. These findings provide insight into the mechanisms underlying the cell cycle effects of lovastatin and support the development of a novel therapeutic strategy directed toward altering deleterious cell proliferation.
Natural Course of Diabetic Nephropathy (2001)

The natural progression of diabetic nephropathy is essentially characterised by a rise of albumin excretion in the urine, a decrease of glomerular filtration rate and an increase in blood pressure. As a rule, terminal renal failure takes years to develop and is classified in five stages depending on the clinical and functional changes. The risk of nephropathy is similar in patients with type 1 and type 2 diabetes: 30% to 50% develop an advanced stage of nephropathy. The propensity to develop nephropathy differs in the population groups and therefore also appears to be genetically determined. The progression of nephropathy development is influenced by numerous factors that can be modified by therapeutic intervention: control of blood sugar and blood pressure, smoking, protein intake, hyperlipidemia and proteinuria.
Hyperspectral imaging for intraoperative diagnosis of colon cancer metastasis in a liver (2019)

Hyperspectral imaging (HSI) is being shown as an emerging modality with a great potential in disease diagnosis and surgical cancer resection. Herein, we evaluate feasibility of the HSI to discriminate and diagnose colon cancer metastasis in a liver from five hematoxylin and eosin stained histopathological specimens. They were collected from the same patient during intraoperative frozen section analysis. Cancer and non-cancer spectra along with corresponding spatial maps were estimated from hyperspectral images by means of spectral unmixing. It was found that maximal angle between cancer spectra is 1.02 degrees less than minimal angle between cancer vs. non-cancer spectra. Thus, spectrum angle mapper was used for pixel-based diagnosis of cancer yielding sensitivity between 81.23% and 97.12%, specificity between 85.85% and 97.3%, and accuracy between 86.85% and 96.92%.
A New Digital Coherent Demodulator (1973)

A coherent demodulator is described which is particularly well suited to digital implementation. Its important features are that no low-pass filter is required to eliminate double-frequency terms and that the passband signal need be sampled at only twice the highest frequency of the baseband signal, rather than at twice the highest frequency of the passband signal. A demodulator of this type will be useful in those systems that do not require passband digital signal processing for other purposes.
Serial Lymphocyte Subpopulation Analysis in Peripheral Blood from a Patient with Amyopathic Dermatomyositis (1994)

Journal Article Serial Lymphocyte Subpopulation Analysis in Peripheral Blood from a Patient with Amyopathic Dermatomyositis Get access S. WAKINO, S. WAKINO *Department of Medicine, Keio University School of MedicineTokyo, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar M. KUWANA, M. KUWANA *Department of Medicine, Keio University School of MedicineTokyo, Japan Correspondence to: M. Kuwana, Division of Rheumatology and Clinical Immunology, Department of Medicine, University of Pittsburgh School of Medicine, 985 Scaife Hall, Pittsburgh, Pennsylvania 15261, U.S.A. Search for other works by this author on: Oxford Academic PubMed Google Scholar T. TOJO, T. TOJO *Department of Medicine, Keio University School of MedicineTokyo, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Y. KAWAI Y. KAWAI †Department of Laboratory Medicine, Keio University School of MedicineTokyo, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Rheumatology, Volume 33, Issue 5, May 1994, Pages 498–499, https://doi.org/10.1093/rheumatology/33.5.498 Published: 01 May 1994 Article history Accepted: 25 November 1993 Published: 01 May 1994
Exploratory study of effective chemotherapy to clear cell carcinoma of the ovary. (2000)

Although clear cell carcinoma of the ovary is considered to be a tumor with poor prognosis, the clinical characteristics has not been defined. The aim of this study was to evaluate the response of clear cell carcinoma of the ovary to first and second-line chemotherapy and explore effective chemotherapy. Fifty-three patients with clear cell carcinoma of the ovary were enrolled between 1988 and 1997 at our department. Since taxol was not available in Japan at that time, cisplatin-based combination chemotherapy has been exclusively used as a standard first-line chemotherapy. Retrospective analyses of clinical characteristics and the response to first or second-line chemotherapy were performed. Median age was 52 years (range 27-71 years). Tumors were 34% (18/53) stage I, 19% (5/53) stage II, 38% (20/53) stage III, and 9% (5/53) stage IV. All patients with I or II stage disease had optimal cytoreduction. Out of 25 patients with III or IV stage disease 20% (5/25) had negative residual tumor, 36% (9/25) had <2 cm residual tumor, and 44% (11/25) had >/=2 cm residual tumor. All patients received postoperative platinum-based chemotherapy. Of 23 patients with measurable residual tumor 8.7% (2/23) completely and 13% (3/23) partially responded to first-line chemotherapy consisting of cisplatin, adriamycin and cyclophosphamide (CAP) or cisplatin and cyclophosphamide (CP) by CT scan or second look laparotomy. Presence of endometriosis was 55% (29/53) but was not a prognostic factor. Although overall response rate of ovarian clear cell carcinoma to first-line chemotherapy by CAP or CP was about 22%, EP or EJ consisting of etoposide and cisplatin or carboplatin used as a second-line chemotherapy showed 29% response rate, while CPT-P consisting of CPT-11 and cisplatin showed 40% response rate. Clear cell carcinomas were frequently present at early stage, with association of endometriosis and with poor overall prognosis. Although patients with advanced ovarian clear cell carcinoma seemed to have better response to CPT-P than conventional platinum-based chemotherapy, further studies are required with larger number of patients to draw firm conclusions.
Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dystrophy (2009)

Duchenne muscular dystrophy (DMD) is a devastating neuromuscular disease caused by mutations in the gene encoding dystrophin. Loss of dystrophin results in reduced sarcolemmal integrity and increased susceptibility to muscle damage. The alpha(7)beta(1)-integrin is a laminin-binding protein up-regulated in the skeletal muscle of DMD patients and in the mdx mouse model. Transgenic overexpression of the alpha(7)-integrin alleviates muscle disease in dystrophic mice, making this gene a target for pharmacological intervention. Studies suggest laminin may regulate alpha(7)-integrin expression. To test this hypothesis, mouse and human myoblasts were treated with laminin and assayed for alpha(7)-integrin expression. We show that laminin-111 (alpha(1), beta(1), gamma(1)), which is expressed during embryonic development but absent in normal or dystrophic skeletal muscle, increased alpha(7)-integrin expression in mouse and DMD patient myoblasts. Injection of laminin-111 protein into the mdx mouse model of DMD increased expression of alpha(7)-integrin, stabilized the sarcolemma, restored serum creatine kinase to wild-type levels, and protected muscle from exercised-induced damage. These findings demonstrate that laminin-111 is a highly potent therapeutic agent for the mdx mouse model of DMD and represents a paradigm for the systemic delivery of extracellular matrix proteins as therapies for genetic diseases.