gebhart/patrepeval huggingface dataset:
The occurrence and formation of monoterpenes in herbivore-damaged poplar roots (2018)

Volatiles are often released upon herbivory as plant defense compounds. While the formation of volatiles above-ground has been intensively studied, little is known about herbivore-induced root volatiles. Here, we show that cockchafer larvae-damaged roots of Populus trichocarpa and P. nigra release a mixture of monoterpenes, including (-)-α-pinene, (-)-camphene, (-)-β-pinene, p-cymene, and 1,8-cineole. Three terpene synthases, PtTPS16 and PtTPS21 from P. trichocarpa and PnTPS4 from P. nigra, could be identified and characterized in vitro. PnTPS4 was found to produce 1,8-cineole as sole product. PtTPS16 and PtTPS21, although highly similar to each other, showed different product specificities and produced γ-terpinene and a mixture of (-)-camphene, (-)-α-pinene, (-)-β-pinene, and (-)-limonene, respectively. Four active site residues were found to determine the different product specificities of the two enzymes. The expression profiles of PtTPS16, PtTPS21, and PnTPS4 in undamaged and herbivore-damaged poplar roots generally matched the emission pattern of monoterpenes, indicating that monoterpene emission in roots is mainly determined at the gene transcript level. Bioassays with Phytophtora cactorum (Oomycetes) revealed inhibitory effects of vapor-phase 1,8-cineole and (-)-β-pinene on the growth of this important plant pathogen. Thus herbivore-induced volatile monoterpenes may have a role in defense against pathogens that cause secondary infections after root wounding.
Analysis of a constant voltage anemometer circuit (2002)

A new circuit arrangement using an operational amplifier that maintains a constant voltage across a sensor connected between a node in the circuit and ground is described. The basic circuit consists of a T resistor network in the feedback of an operational amplifier where the sensor is connected in place of the center grounded element. The analysis of such a circuit arrangement is presented for a particular application to a hot-wire/film anemometer. An introduction is given to focus on the requirements of such a circuit to function as an anemometer, considering the hot-wire as a first order low-pass filter due to its thermal inertia. An innovative internal compensation method for the thermal inertia effects is presented which would extend the bandwidth. The expression for the overall bandwidth of such a circuit is derived with its sensitivity. An approach to extend the bandwidth even further using a composite amplifier configuration is presented. Experimental results showing excellent agreement with conventional approaches are presented. The advantage of the constant voltage operation in terms of insignificant cable capacitance effects and higher signal to noise ratio is discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Synthesis of cembrene. 14-Membered ring diterpene (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of cembrene. 14-Membered ring diterpeneW. G. Dauben, G. H. Beasley, M. D. Broadhurst, B. Muller, D. J. Peppard, P. Pesnelle, and C. SuterCite this: J. Am. Chem. Soc. 1974, 96, 14, 4724–4726Publication Date (Print):July 1, 1974Publication History Published online1 May 2002Published inissue 1 July 1974https://pubs.acs.org/doi/10.1021/ja00821a087https://doi.org/10.1021/ja00821a087research-articleACS PublicationsRequest reuse permissionsArticle Views204Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Metal-free polymerization: synthesis and properties of fused benzo[1,2-b:4,5-b′]bis[b]benzothiophene (BBBT) polymers (2020)

A family of fused semiconducting polymers containing the thienoacenes <bold>BBBT</bold> has been synthesized efficiently by non-metal and environmentally benign polymerization.
An Interdisciplinary Approach and Characterization of Neuronal Cells Transdifferentiated from Human Mesenchymal Stem Cells (2007)

Mesenchymal stem cells (MSCs) are mesoderm-derived cells, primarily resident in adult bone marrow (BM). MSCs exhibit lineage differentiation to generate cells such as BM stroma, fat, and cartilage. Recent studies have reported the transdifferentiation of MSCs to cells of ectodermal and endodermal origin. Previously, we have reported transdifferentiation of human (h) MSCs into neuronal cells using retinoic acid (RA) as a differentiating agent. This study presents a more efficient induction method and rigorously characterizes the development using molecular, cellular, and functional approaches. A cocktail of induction agents containing basic fibroblast growth factor (bFGF) and RA generated cells that expressed glial and neuronal progenitor markers (GFAP) at day 2 post-induction. By day 12, 90% of hMSCs differentiated into cells that expressed markers consistent for neurons, including transcription factors linked to the development of differentiated neurons. Furthermore, cell proliferation studies and western blots for cell cycle-specific proteins demonstrated day-12 induced cells to be post-mitotic cells with no evidence of cell death. The cells exhibited spontaneous post-synaptic currents and were capable of neurotransmitter synthesis, packaging, and release. Together, the improved induction protocol, combined with an interdisciplinary approach to verify that hMSCs can differentiate into neuronal cells, provides a step toward translational application with models of regenerative medicine.
Analysis of pitch accent categories in Welsh (1999)

This paper describes the results of a study of pitch accent categories in Welsh, and their distribution in natural speech. In addition to these descriptive findings, it describes the development of a data-driven approach to intonation. To be useful for the analysis of natural speech, an intonational model must provide means for determining an unambiguous phonological representation for any observed speech token. One approach which will be addressed is the Tilt Model [K. Dusterhoff and A. Black, ESCA Workshop on Intonation (1997); P. Taylor, ICSLP (1998)], amended as necessary to account for the accent patterns of Welsh. This will be compared with an alternative of using statistical methods, particularly factor analysis, to determine the underlying descriptive dimensions of pitch accents in the language. The resulting model will be applied to a corpus of spoken Welsh utterances, and initial findings on the distribution of accent types will be reported.
Buffered formalin is the superior fixative for the detection of HPV DNA by in situ hybridization analysis. (1989)

In situ hybridization is used commonly for detection of human papillomavirus (HPV) DNA. There is little information, however, on whether the detection of HPV DNA by in situ hybridization can be affected by the way in which the tissue is fixed. To address this question, the authors compared the hybridization signal using this technique under low stringency conditions for several genital condylomata containing HPV 6 or 11 that were randomly subdivided and fixed in various fixatives for 16 hours. In all cases, the largest proportion of cells with koilocytotic atypia that had detectable HPV DNA was in buffered formalin-fixed tissue (80%), followed by tissue fixed in unbuffered formalin (70%), Hartman's solution (40%), and Bouin's solution (10%). After a high stringency wash, the greatest decrease in the overall hybridization signal was with tissue fixed in Bouin's solution; a minimal decrease was noted with tissue fixed in buffered formalin. Fixation in Bouin's solution for 2 hours gave in situ hybridization results comparable with buffered formalin fixation but with poorer cytologic detail. It is concluded that, of the fixatives studied, buffered formalin is superior for the detection of HPV DNA by in situ hybridization analysis.
The observation of intercombination lines 1s3p3P1→1s21S0of multicharged He-like ions in laser-produced plasmas (1977)

The intercombination lines 1s3p 3P1 to 1s2 1S0 of the ions Mg XI, Al XII, P XIV, S XV and Cl XVI have been observed for the first time in the spectra of laser-produced plasmas. The wavelengths and relative intensities of such lines were measured. The identification of the observed lines was carried out by means of a comparison of the experimental wavelengths and intensities with the theoretical ones. The dependence of the relative intensities of such lines on the plasma parameters was investigated.
Management of Acute Traumatic Central Cord Syndrome (ATCCS) (2013)

ABBREVIATIONS: ASIA, American Spinal Injury Association ATCCS, acute traumatic central cord syndrome
PX‐478, an inhibitor of hypoxia‐inducible factor‐1α, enhances radiosensitivity of prostate carcinoma cells (2008)

Overexpression of hypoxia-inducible factor-1alpha (HIF-1alpha) in human tumors is associated with poor prognosis and poor outcome to radiation therapy. Inhibition of HIF-1alpha is considered as a promising approach in cancer therapy. The purpose of this study was to test the efficacy of a novel HIF-1alpha inhibitor PX-478 as a radiosensitizer under normoxic and hypoxic conditions in vitro. PC3 and DU 145 prostate carcinoma cells were treated with PX-478 for 20 hr, and HIF-1alpha protein level and clonogenic cell survival were determined under normoxia and hypoxia. Effects of PX-478 on cell cycle distribution and phosphorylation of H2AX histone were evaluated. PX-478 decreased HIF-1alpha protein in PC3 and DU 145 cells. PX-478 produced cytotoxicity in both cell lines with enhanced toxicity under hypoxia for DU-145. PX-478 (20 mumol/L) enhanced the radiosensitivity of PC3 cells irradiated under normoxic and hypoxic condition with enhancement factor (EF) 1.4 and 1.56, respectively. The drug was less effective in inhibiting HIF-1alpha and enhancing radiosensitivity of DU 145 cells compared to PC3 cells with EF 1.13 (normoxia) and 1.25 (hypoxia) at 50 mumol/L concentration. PX-478 induced S/G2M arrest in PC3 but not in DU 145 cells. Treatment of PC3 and DU 145 cells with the drug resulted in phosphorylation of H2AX histone and prolongation of gammaH2AX expression in the irradiated cells. PX-478 is now undergoing Phase I clinical trials as an oral agent. Although the precise mechanism of enhancement of radiosensitivity remains to be identified, this study suggests a potential role for PX-478 as a clinical radiation enhancer.
Pericardial Injection of Kainic Acid Induces a Chronic Epileptic State in Larval Zebrafish (2021)

Epilepsy is a common disorder of the brain characterized by spontaneous recurrent seizures, which develop gradually during a process called epileptogenesis. The mechanistic processes underlying the changes of brain tissue and networks toward increased seizure susceptibility are not fully understood. In rodents, injection of kainic acid (KA) ultimately leads to the development of spontaneous epileptic seizures, reflecting similar neuropathological characteristics as seen in patients with temporal lobe epilepsy (TLE). Although this model has significantly contributed to increased knowledge of epileptogenesis, it is technically demanding, costly to operate and hence not suitable for high-throughput screening of anti-epileptic drugs (AEDs). Zebrafish, a vertebrate with complementary advantages to rodents, is an established animal model for epilepsy research. Here, we generated a novel KA-induced epilepsy model in zebrafish larvae that we functionally and pharmacologically validated. KA was administered by pericardial injection at an early zebrafish larval stage. The epileptic phenotype induced was examined by quantification of seizure-like behavior using automated video recording, and of epileptiform brain activity measured via local field potential (LFP) recordings. We also assessed GFP-labeled GABAergic and RFP-labeled glutamatergic neurons in double transgenic KA-injected zebrafish larvae, and examined the GABA and glutamate levels in the larval heads by liquid chromatography with tandem mass spectrometry detection (LC-MS/MS). Finally, KA-injected larvae were exposed to five commonly used AEDs by immersion for pharmacological characterization of the model. Shortly after injection, KA induced a massive damage and inflammation in the zebrafish brain and seizure-like locomotor behavior. An abnormal reorganization of brain circuits was observed, a decrease in both GABAergic and glutamatergic neuronal population and their associated neurotransmitters. Importantly, these changes were accompanied by spontaneous and continuous epileptiform brain discharges starting after a short latency period, as seen in KA rodent models and reminiscent of human pathology. Three out of five AEDs tested rescued LFP abnormalities but did not affect the seizure-like behavior. Taken together, for the first time we describe a chemically-induced larval zebrafish epilepsy model offering unique insights into studying epileptogenic processes <i>in vivo</i> and suitable for high-throughput AED screening purposes and rapid genetic investigations.
Serum Gastrin in Patients with Peptic Ulceration (1970)

The fasting serum level of gastrin was determined by radioimmunoassay in 41 normal subjects, 27 patients with duodenal ulceration, 12 patients with gastric ulceration, and 8 patients following "complete" vagotomy. The patients with duodenal ulceration had significantly higher serum levels of gastrin (1.3 +/- S.D. 0.7 ng./ml.) than normal subjects (0.4 +/- S.D. 0.3 ng./ml.), patients with gastric ulceration (0.4 +/- S.D. 0.4 ng./ml.), or post-vagotomy patients (0.15 +/- S.D. 0.2 ng./ml.).The gastrin secretion in both normal subjects and patients with duodenal ulceration was responsive to protein ingestion, but a larger incremental secretion of gastrin occurred in the latter group. Hyperglycaemia significantly suppressed the serum level of gastrin in both groups. The patients with gastric ulceration had fasting and postprandial serum gastrin levels not significantly different from normal subjects.
The Field of a Noise Source above an Infinite Plane (1955)

The sound field from a random noise source above ground as measured by a receiver with finite band width is studied both theoretically and experimentally. For simplicity, only the far field has been considered. The special case of a perfectly reflecting plane is discussed first, and nondimensional curves are presented of sound pressure level versus distance for two different receiver band widths. The analysis is then extended to a plane of arbitrary normal impedance, and pressure level versus distance curves are given for typical field operating conditions.
Highly twisted supercoils for superelastic multi-functional fibres (2019)

Highly deformable and electrically conductive fibres with multiple functionalities may be useful for diverse applications. Here we report on a supercoil structure (i.e. coiling of a coil) of fibres fabricated by inserting a giant twist into spandex-core fibres wrapped in a carbon nanotube sheath. The resulting supercoiled fibres show a highly ordered and compact structure along the fibre direction, which can sustain up to 1,500% elastic deformation. The supercoiled fibre exhibits an increase in resistance of 4.2% for stretching of 1,000% when overcoated by a passivation layer. Moreover, by incorporating pseudocapacitive-active materials, we demonstrate the existence of superelastic supercapacitors with high linear and areal capacitance values of 21.7 mF cm<sup>-1</sup> and 92.1 mF cm<sup>-2</sup>, respectively, that can be reversibly stretched by 1,000% without significant capacitance loss. The supercoiled fibre can also function as an electrothermal artificial muscle, contracting 4.2% (percentage of loaded fibre length) when 0.45 V mm<sup>-1</sup> is applied.
Communities of design practice in electronic government (2003)

As we move towards more online governmental services-electronic government or e-government-our information technology (IT) and human-computer interaction (HCI) designs must both broaden and deepen. In Georgia, we have fostered communities of design practice (CDPs) that focus on IT and HCI design at the statewide enterprise level through the Georgia Digital Academy (GDA). In this study, we utilized observational methods and a participatory analysis of the CDP formation in the GDA. Our findings and conclusions converge with the growing body of knowledge on HCI communities of practice. We report our results and recommendations on how CDPs might be utilized to build effective e-government.
Deferred autologous stem cell transplantation in systemic AL amyloidosis (2018)

High-dose melphalan with autologous stem cell transplantation (ASCT) can induce durable haematological and organ responses in systemic AL amyloidosis (AL). Stringent selection criteria have improved safety of ASCT in AL but most patients are transplant-ineligible. We report our experience of deferred ASCT in AL patients who were transplant-ineligible at presentation but had improvements in organ function after induction chemotherapy, enabling them to undergo ASCT. Twenty-two AL patients underwent deferred ASCT from 2011 to 2017. All had serial organ function and clonal response assessment. Organ involvement and responses were defined by amyloidosis consensus criteria. All patients were transplant-ineligible at presentation, predominantly due to advanced cardiac involvement. All received bortezomib-based therapy, with 100% haematologic response (86% complete response (CR)/very good partial response (VGPR)), enabling reversal of ASCT exclusion criteria. Patients underwent deferred ASCT for haematologic progression (45%) or consolidation (55%). There was no transplant-related mortality. Haematologic responses post-ASCT: CR 50%, VGPR 27%, PR 18%, non-response 5%. In all, 85.7% achieved cardiac responses. Median overall survival (OS) was not reached. Median progression-free survival (PFS) was 54 months. This selected cohort achieved excellent haematologic responses, organ responses, PFS and OS with deferred ASCT. If larger studies confirm these findings, this may widen the applicability of ASCT in AL.
Cultivation of immortalized human hepatocytes HepZ on macroporous CultiSpher G microcarriers (2000)

Cultivation of the new immortalized hepatocyte cell line HepZ was performed with a 1:1 mixture of DMEM and Ham's F12 media containing 5% FCS. The cells were grown in their 40th passage in 100 mL and 1 L volumes in spinner flasks and in a bioreactor, respectively. For the production of adherently growing HepZ cells macroporous CultiSpher G gelatin microcarriers were used in various concentrations from 1 to 3 g/L. The cells were seeded in a density of 2 x 10(5) cells/mL when using a microcarrier concentration of 1 g/L and 5 x 10(5) cells/mL at a microcarrier concentration of 3 g/L. After 7 days of cultivation a maximum cell concentration of 4.5 x 10(6) cells/mL was obtained in the spinner culture using a microcarrier concentration of 1 g/L. With bubble-free aeration and daily medium exchange from day 7, 7.1 x 10(6) cells/mL were achieved in the bioreactor using a microcarrier concentration of 3 g/L. The cells exhibited a maximum specific growth rate of 0.84 per day in the spinner system and 1.0 per day in the bioreactor, respectively. During the growth phase the lactate dehydrogenase (LDH) activity rose slightly up to values of 200 U/L. At the end of cultivation the macroporous carriers were completely filled with cells exhibiting a spherical morphology whereas the hepatocytes on the outer surface were flat-shaped. Concerning their metabolic activity the cells predominantly consumed glutamine and glucose. During the growth phase lactate was produced up to 19.3 mM in the spinner culture and up to 9.1 mM in the bioreactor. Maximal oxygen consumption was 1950 nmol/(10(6) cells. day). HepZ cells resisted a 4-day long chilling period at 9.5 degrees C. The cytochrome P450 system was challenged with a pulse of 7 microgram/mL lidocaine at a cell density of 4.5 x 10(6) cells/mL. Five ng/mL monoethylglycinexylidide (MEGX) was generated within 1 day without phenobarbital induction compared to 26 ng/mL after a preceded three day induction period with 50 microgram/mL of phenobarbital indicating hepatic potency. Thus, the new immortalized HepZ cell line, exhibiting primary metabolic functions and appropriate for a mass cell cultivation, suggests its application for a bioartificial liver support system.
The edentulous ridge expansion (ERE) technique an experimental study in the dog (2013)

Implants installed by applying an ERE technique may osseointegrate similarly to conventional implant installation. However, vertical and horizontal resorption of the displaced buccal bony wall occurred as well.
A robust multisource image automatic registration system based on the SIFT descriptor (2011)

Image registration is an essential step in many remote-sensing (RS) applications. This article presents a study of a multisource image automatic registration system (MIARS) based on the scale-invariant feature transform (SIFT), which has been demonstrated to be the most robust local invariant feature descriptor for automatically registering various RS images. The SIFT descriptor has two shortcomings: it is unsuitable for extremely large images and has an irregular distribution of feature points. Therefore, three steps are proposed for the MIARS: image division, histogram equalization and the elimination of false point matches by a subregion least squares iteration. Image division makes it possible to use the SIFT descriptor to extract control points from an extremely large RS image. Histogram equalization in prematching improves the contrast sensitivity of RS images. The subregion least squares iteration refines the registration accuracy. Images from multisensor systems, including Quickbird, IRS-P6, Landsat/TM, HJ-CCD, HJ-IRS, light detection and ranging (LiDAR) intensity images and aerial data, were selected to test the reliability of the MIARS. The results indicated that better registration accuracy was achieved, which will be very helpful in the future development of a registration model.
Performance of distance protection of e.h.v. feeders utilising shunt - reactor arrangements for arc suppression and voltage control (1980)

Digital techniques for modelling the faulted response of any arbitrary linear shunt-reactor compensated e.h.v. transmission-line system are described. The methods are specifically developed to meet the requirements of offline or programmable test-equipment-based feeder-protection studies. The essential differences in the faulted response of single and 3-section feeders are established by reference to 500 kV interconnections involving 4-reactor linear, shunt compensators. An extensive study of modern distance protection applied to various feeder configurations is reported and the paper concludes with an evaluation of protection performance in a typical sectionalised interconnector of length 550 km.
Two different cationic positions in Cu-SSZ-13? (2012)

H(2)-TPR and FTIR were used to characterize the nature of the Cu ions present in the Cu-SSZ-13 zeolite at different ion exchange levels. The results obtained are consistent with the presence of Cu ions at two distinct cationic positions in the SSZ-13 framework.