gebhart/patrepeval huggingface dataset:
Determination and Imaging of Small Biomolecules and Ions Using Ruthenium(II) Complex-Based Chemosensors (2022)

Luminescence chemosensors are one of the most useful tools for the determination and imaging of small biomolecules and ions in situ in real time. Based on the unique photo-physical/-chemical properties of ruthenium(II) (Ru(II)) complexes, the development of Ru(II) complex-based chemosensors has attracted increasing attention in recent years, and thus many Ru(II) complexes have been designed and synthesized for the detection of ions and small biomolecules in biological and environmental samples. In this work, we summarize the research advances in the development of Ru(II) complex-based chemosensors for the determination of ions and small biomolecules, including anions, metal ions, reactive biomolecules and amino acids, with a particular focus on binding/reaction-based chemosensors for the investigation of intracellular analytes' evolution through luminescence analysis and imaging. The advances, challenges and future research directions in the development of Ru(II) complex-based chemosensors are also discussed.
Microstructure of Nano-Crystallized Ni-P Layers Obtained by a Chemical Method (2005)

The present paper is focused on the microstructure of thin layers the Ni-P subjected to nano-crystallization from the amorphous state. The layers in this study have been produced by a chemical reduction method and crystallized in order to obtain mixed amorphous-crystalline and nano-polycrystalline microstructures. The results of investigation of microstructures obtained by annealing at the temperatures 270°C and 320°C, i.e. below the peak of crystallization (370°C), are described. The X-ray study, calorimetric analysis and microstructure observations carried out by the transmission electron microscopy revealed early stages of crystallization in amorphous state and changes in the size and shape of grains. Crystals of different sizes have been observed in crystallized materials, some of them in the nano-metres range. A correlation has been found between the P concentration in amorphous Ni-P material and the microstructure at the front of crystallization. It has been observed that crystallization process starts in the area of lower concentration of the phosphorous. The crystals in these regions have an elongated shape and form bands separated by amorphous material.
Serine/threonine kinase akt activation regulates the activity of retinal serine/threonine phosphatases, PHLPP and PHLPPL (2010)

In our previous studies, we have shown that insulin receptor (IR) activation leads to the activation of phosphoinositide 3-kinase (PI3K) and Akt activation in rod photoreceptors. This pathway is functionally important for photoreceptor survival as deletion of IR and one of the isoforms of Akt (Akt2) resulted in stress-induced photoreceptor degeneration. However, the molecular mechanism of this degeneration is not known. Akt signaling is known to be regulated by the serine/threonine phosphatases, PH domain and leucine-rich repeat protein phosphatases (PHLPP) and PHLPP-like (PHLPPL). In this study, we characterized these two phosphatases in the retina and examined the role of IR, PI3K, and Akt signaling on the activity of PHLPP and PHLPPL. Most of the studies published on PHLPP and PHLPPL are directed toward Akt dephosphorylation; however, there are no studies available to date on how the enzyme activities of these phosphatases are regulated. We made a novel finding in this study that both PHLPP and PHLPPL activities were significantly decreased in the presence of insulin ex vivo. The insulin-induced decrease of phosphatase activities were PI3K-dependent as pre-treatment of ex vivo retinal cultures with LY294002 significantly reversed the insulin-induced inhibition. It has been shown previously that PHLPP and PHLPPL regulate the dephosphorylation of Akt isoforms, and our results demonstrate for the first time that retinal PHLPP and PHLPPL activities are under the control of the IR-activated PI3K/Akt pathway.
Development and experimental validation of fault detection and diagnosis method in SPWM modulated symmetric cascaded H-bridge multilevel inverter (2020)

This paper discusses gate open-circuit fault detection and diagnosis method in SPWM modulated symmetric cascaded H-bridge multilevel inverter. First, an output voltage modelling under fault condition in cascaded H-bridge multilevel inverter is presented. Further, simulations of fault conditions have been carried out for the development of the fault detection and diagnosis method. Based on fault modelling and simulations, a fault detection and diagnosis method have been proposed based on the mean values of the bridge voltages as the diagnostic feature. Further, the proposed method has been validated experimentally on a 5-level symmetric sine pulse width modulated cascaded H-bridge multilevel inverter.
Tolerability of Aquaretic-Related Symptoms Following Tolvaptan for Autosomal Dominant Polycystic Kidney Disease: Results From TEMPO 3:4 (2017)

In this study, AAEs were common but well tolerated in ADPKD patients on tolvaptan. ADPKD patients in earlier stages of disease progression may be more sensitive to aquaretic symptoms, which may help in guiding tolvaptan dosing and titration decisions in the future.
Application of area scaling analysis to identify natural killer cell and monocyte involvement in the GranToxiLux antibody dependent cell‐mediated cytotoxicity assay (2018)

Several different assay methodologies have been described for the evaluation of HIV or SIV-specific antibody-dependent cell-mediated cytotoxicity (ADCC). Commonly used assays measure ADCC by evaluating effector cell functions, or by detecting elimination of target cells. Signaling through Fc receptors, cellular activation, cytotoxic granule exocytosis, or accumulation of cytolytic and immune signaling factors have been used to evaluate ADCC at the level of the effector cells. Alternatively, assays that measure killing or loss of target cells provide a direct assessment of the specific killing activity of antibodies capable of ADCC. Thus, each of these two distinct types of assays provides information on only one of the critical components of an ADCC event; either the effector cells involved, or the resulting effect on the target cell. We have developed a simple modification of our previously described high-throughput ADCC GranToxiLux (GTL) assay that uses area scaling analysis (ASA) to facilitate simultaneous quantification of ADCC activity at the target cell level, and assessment of the contribution of natural killer cells and monocytes to the total observed ADCC activity when whole human peripheral blood mononuclear cells are used as a source of effector cells. The modified analysis method requires no additional reagents and can, therefore, be easily included in prospective studies. Moreover, ASA can also often be applied to pre-existing ADCC-GTL datasets. Thus, incorporation of ASA to the ADCC-GTL assay provides an ancillary assessment of the ability of natural and vaccine-induced antibodies to recruit natural killer cells as well as monocytes against HIV or SIV; or to any other field of research for which this assay is applied. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of ISAC.
ARF: Artistic Radiance Fields (2022)

We present a method for transferring the artistic features of an arbitrary style image to a 3D scene. Previous methods that perform 3D stylization on point clouds or meshes are sensitive to geometric reconstruction errors for complex real-world scenes. Instead, we propose to stylize the more robust radiance field representation. We find that the commonly used Gram matrix-based loss tends to produce blurry results without faithful brushstrokes, and introduce a nearest neighbor-based loss that is highly effective at capturing style details while maintaining multi-view consistency. We also propose a novel deferred back-propagation method to enable optimization of memory-intensive radiance fields using style losses defined on full-resolution rendered images. Our extensive evaluation demonstrates that our method outperforms baselines by generating artistic appearance that more closely resembles the style image. Please check our project page for video results and open-source implementations: https://www.cs.cornell.edu/projects/arf/ .
Inhibition of Varicella-Zoster Virus (VZV) Glycoprotein Expression by a Human Monoclonal Antibody against VZV Glycoprotein III (1993)

Effects of human monoclonal antibodies (MAbs) against varicella-zoster virus (VZV) glycoproteins (gp) on VZV glycoprotein expression were analyzed by flow cytometry. Human embryonic fibroblast (HEF) cells were inoculated with cell-free VZV and cultured in the presence of human MAbs. While human MAbs directed against gpI (V2) and -II (V1) did not affect VZV expression, a human MAb against gpIII (V3) inhibited expression of VZV gpI, -II, -III, and -IV. V3 also inhibited spread of VZV from infected to uninfected cells. Interferon-alpha, -beta, and -gamma and tumor necrosis factor-alpha were able to synergize with V3 and inhibit VZV gp expression. These results suggest that human MAb V3 may be useful for passive prophylaxis against VZV infection and for treatment of severe VZV infection.
Expression profiling and proteomic analysis of isolated photosynthetic cells of the non-Kranz C4 species Bienertia sinuspersici (2010)

Bienertia sinuspersici Akhani represents one form of C4 photosynthesis that occurs without Kranz anatomy in family Chenopodiaceae. Analysis of transcript profiles and proteomics were made to gain information on this single-cell C4 photosynthetic mechanism. Chlorenchyma cells were isolated and purified from mature leaves. From these cells, a cDNA library was made from which sequences were obtained on 2385 clones using conventional methods. To obtain a protein profile, the multi dimensional protein identification technique was used, resulting in identification of 322 unique proteins in chlorenchyma cells. After analysing datasets from the EST library and proteomics, genes and proteins were classified into 23 and 17 categories according to types of biological processes, respectively. These include photosynthesis and photorespiration, other biosynthetic and metabolic processes, cell wall modification, defence response, DNA repair, electron transport, other cellular and developmental processes, protein folding, protein targeting, protein modification, proteolysis, redox and ion homeostasis, response to biotic and abiotic stresses, RNA modification, transcription, translation, transport and unknowns. Sequence and phylogenetic analyses were made of C4 cycle enzymes to characterise the relationship between homologues found in Bienertia with public gene sequences from other chenopods and representative C3 and C4 species from other families. Identified photosynthetic genes and proteins are discussed with respect to the proposed function of an NAD-ME type C4 cycle in this single-cell C4 system.
Entrapping Intermediates of Thermal Aggregation in α-Helical Proteins with Low Concentration of Guanidine Hydrochloride (2000)

Aggregation of proteins is a problem with serious medical implications and economic importance. To develop strategies for preventing aggregation, the mechanism(s) and pathways by which proteins aggregate must be characterized. In this study, the thermally induced aggregation processes of three alpha-helix proteins (myoglobin, cytochrome c, and lysozyme) in the presence and absence of 1.0 m guanidine hydrochloride (GdnHCl) were investigated by means of infrared spectroscopy. In the absence of GdnHCl, intensities of the alpha-helix bands (approximately 1656 cm(-1)) decrease as a function of temperature at above 50 degrees C. With myoglobin and cytochrome c, the loss of helix bands was accompanied by the appearance of two new bands at 1694 and 1623 cm(-1), indicative of the formation of intermolecular beta-sheet aggregates. For lysozyme, bands indicative of intermolecular beta-sheet aggregates did not appear in any significant intensity. In the presence of 1.0 m GdnHCl, two major intermediate states rich in 3(10)-helix (represented by the band at 1663 cm(-1)) and beta-turn structure (represented by the band at 1667 cm(-1)), respectively, were observed. These findings demonstrated that IR spectroscopic studies of protein aggregation using a combination of thermal and chemical denaturing factors could provide a means to populate and characterize aggregation intermediates.
Differential proliferative effects of transforming growth factor- beta on human hematopoietic progenitor cells. (1988)

Transforming growth factor-beta (TGF beta) regulates cell growth and differentiation in numerous cell systems, including several hematopoietic lineages. We used in vitro cultures of highly enriched hematopoietic progenitor cells stimulated by natural and recombinant growth factors to investigate the biologic effects of TGF beta 1 and TGF beta 2 on erythroid (CFU-E and burst-forming unit (BFU)-E), granulocyte-macrophage (CFU-GM) and multilineage (i.e., granulocyte, erythroid, macrophage, and megakaryocyte; CFU-GEMM) colony-forming cells. In the absence of exogenous CSF, neither TGF beta 1 nor TGF beta 2 supported progenitor cell growth. In the presence of recombinant or natural CSF, picomolar concentrations of TGF beta 1 inhibited growth of CFU-E, BFU-E, and CFU-GEMM and enhanced growth of day 7 CFU-GM. Inhibition of CFU-E and BFU-E by human and porcine TGF beta 1 was similar, ranging from 17 to 73% over a concentration range of 0.05 to 1.0 ng/ml, and was largely independent of the type of burst-promoting activity used (rIL-3 vs cell line 5637-conditioned medium). Inhibition of CFU-GEMM ranged from 79 to 98% over a concentration range of 0.25 to 1.0 ng/ml. The inhibitory effect of TGF beta 1 was progressively lost when its addition was delayed for 40 to 120 h, suggesting a mode of action during early cell divisions. In contrast, growth of CFU-GM stimulated by plateau concentrations of human rG-CSF, rGM-CSF, and rIL-3 was enhanced up to 154 +/- 22% by human TGF beta 1. Porcine platelet-derived TGF beta 2 was essentially without effect on the progenitor populations examined. These results support the hypothesis that TGF beta may play role in the regulation of hematopoietic progenitor cell proliferation by differentially affecting individual lineages and is apparently capable of doing so in the relative absence of marrow accessory cells.
Derivation of the multiply‐branched ergot alkaloid pathway of fungi (2023)

Ergot alkaloids are a large family of fungal specialized metabolites that are important as toxins in agriculture and as the foundation of powerful pharmaceuticals. Fungi from several lineages and diverse ecological niches produce ergot alkaloids from at least one of several branches of the ergot alkaloid pathway. The biochemical and genetic bases for the different branches have been established and are summarized briefly herein. Several pathway branches overlap among fungal lineages and ecological niches, indicating activities of ergot alkaloids benefit fungi in different environments and conditions. Understanding the functions of the multiple genes in each branch of the pathway allows researchers to parse the abundant genomic sequence data available in public databases in order to assess the ergot alkaloid biosynthesis capacity of previously unexplored fungi. Moreover, the characterization of the genes involved in the various branches provides opportunities and resources for the biotechnological manipulation of ergot alkaloids for experimentation and pharmaceutical development.
Bound to Plan: Exploiting Classical Heuristics via Automatic Translations of Tail-Recursive HTN Problems (2016)

Hierarchical Task Network (HTN) planning is a formalism that can express constraints which cannot easily be expressed by classical (non-hierarchical) planning approaches. It enables reasoning about procedural structures and domain-specific search control knowledge. Yet the cornucopia of modern heuristic search techniques remains largely unincorporated in current HTN planners, in part because it is not clear how to estimate the goal distance for a partially-ordered task network. When using SHOP2-style progression, a task network of yet unprocessed tasks is maintained during search. In the general case it can grow arbitrarily large. However, many — if not most — existing HTN domains have a certain structure (called tail-recursive) where the network's size is bounded. We show how this bound can be calculated and exploited to automatically translate tail-recursive HTN problems into non-hierarchical STRIPS representations, which allows using both hierarchical structures and classical planning heuristics. In principle, the approach can also be applied to non-tail-recursive HTNs by incrementally increasing the bound. We give three translations with different advantages and present the results of an empirical evaluation with several HTN domains that are translated to PDDL and solved by two current classical planning systems. Our results show that we can automatically find practical bounds for solving partially-ordered HTN problems. We also show that classical planners perform similarly with our automatic translations versus a previous hand-bounded HTN translation which is restricted to totally-ordered problems.
Investigation of the Interaction Between Dentifrice Flavor and Product Base by Principal Component Analysis of Headspace Gas Chromatography (1996)

The extent to which dentifrice flavor interacts with the product's continuous phase and the abrasive profoundly affects how flavor will be perceived. Static headspace-GC analysis was used to examine dentifrice in the neat form and as 50% and 25% (v/v) suspensions in water to simulate the effect of dilution upon brushing. To aid in our investigations of the vapor-liquid equilibria of flavor, data were examined by principal component analysis. Two abrasives and two continuous phase compositions were considered. The two abrasives differ in their ability to complex flavor thus affecting the headspace profile of the neat dentifrice. However, flavor release upon dilution is related to continuous phase composition and is independent of the abrasive composition.
SIGIBE: Solving random bilinear equations via gradient descent with spectral initialization (2016)

We investigate the problem of finding the real-valued vectors h, of size L, and x, of size P, from M independent measurements y <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> = 〈a <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> , h〉〈b <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> , x〉, where a <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> and b <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> are known random vectors. Recovery of the unknowns entails solving a set of bilinear equations, a challenging problem encountered in signal processing tasks such as blind deconvolution for channel equalization or image deblurring. Inspired by the Wirtinger flow approach to the related phase retrieval problem, we propose a solver that proceeds in two steps: (i) first a spectral method is used to obtain an initial guess; which is then (ii) refined using simple and scalable gradient descent iterations to minimize a natural non-convex formulation of the recovery problem. Our method - which we refer to as SIGIBE: Spectral Initialization and Gradient Iterations for Bilinear Equations - can accommodate arbitrary correlations between a <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> and b <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> . Different from recent approaches to blind deconvolution using convex relaxation, SIGIBE does not require matrix lifting that could hinder the method's scalability. Numerical tests corroborate SIGIBE's effectiveness in various data settings, and show successful recovery with as few as M ≳ (L + P) measurements.
Using a Wrist-Worn Device Based on Peripheral Arterial Tonometry to Diagnose Obstructive Sleep Apnea: In-Laboratory and Ambulatory Validation (2004)

In a population of patients suspected of having obstructive sleep apnea, the Watch_PAT can quantify an ODI that compares very well with Medicare criteria for defining respiratory events and an RDI that compares favorably with Chicago criteria for defining respiratory events. The device can be used with a low failure rate for single use in the lab and home for self-administered testing.
Energy supply for remote base transceiver stations of telecommunication (2004)

This paper presents software with graphical user interfaces, developed to make economic studies and a reliability analyse about power supply for remote base transceiver station. These works deal with remote supply like solar panels, wind turbines, fuel cells, microturbines and diesel motors. The main thematic is to compare two solutions witch are supply these telecommunication stations with the electric network and so bring a LV (low voltage) line to this one, or, use remote power supply structures. Different structures with one or several generators can be tested and solutions will be different according to the establishment site and to the ratio cost/reliability desired
The 2nd 3D Face Alignment in the Wild Challenge (3DFAW-Video): Dense Reconstruction From Video (2019)

3D face alignment approaches have strong advantages over 2D with respect to representational power and robustness to illumination and pose. Over the past few years a number of research groups have made rapid advances in dense 3D alignment from 2D video and obtained impressive results. How these various methods compare is relatively unknown. Previous benchmarks addressed sparse 3D alignment and single image 3D reconstruction. No commonly accepted evaluation protocol exists for dense 3D face reconstruction from video with which to compare them. The 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">nd</sup> 3D Face Alignment in the Wild from Videos (3DFAW-Video) Challenge extends the previous 3DFAW 2016 competition to the estimation of dense 3D facial structure from video. It presented a new large corpora of profile-to-profile face videos recorded under different imaging conditions and annotated with corresponding high-resolution 3D ground truth meshes. In this paper we outline the evaluation protocol, the data used, and the results. 3DFAW-Video is to be held in conjunction with the 2019 International Conference on Computer Vision, in Seoul, Korea.