gebhart/patrepeval huggingface dataset:
Flexible microelectrode arrays with integrated insertion devices (2002)

Flexible microelectrode arrays (FMAs) allow interfacing to delicate living tissues such as neural tissue with a minimum of physical disruption of that tissue during and after insertion. This physical disruption is minimized since the compliant FMAs can deform along with the tissue. However, a problem with these arrays is the insertion and subsequent precise positioning of the arrays in the tissue. Previous FMAs required hand assembly of the flexible array with another rigid structure. This may not be feasible if the dimensions of the flexible array are too small. In this work, FMAs with integrated rigid insertion devices were designed, fabricated, and assessed. Thin-film technology and electrodeposition were used to create flexible arrays with attached rigid insertion devices in a single sequence of fabrication steps. These arrays can be designed in two different configurations. The first type allows for flexible electrodes to be sewn through a nerve. The second allows for insertion into a surface such as the cerebral cortex or the spinal cord. After insertion, the rigid portion of the FMA is removed from the tissue with the flexible portion remaining behind. These two implantation schemes were tested on tissue models and found to be straightforward and reliable. In addition, comparisons of the potential to cause tissue damage between flexible and rigid arrays of similar dimensions were made under three different conditions of mechanical perturbation. In all cases, FMAs caused no damage to the tissue model above that caused by the original electrode insertion track while rigid arrays caused significant tearing. Finally, FMAs were shown to successfully stimulate neural tissue in an experimental setting.
Sortase-Mediated Phage Decoration for Analytical Applications (2021)

Phage-borne peptides and antibody fragments isolated from phage display libraries have proven to be versatile and valuable reagents for immunoassay development. Due to the lack of convenient and mild-condition methods for the labeling of the phage particles, isolated peptide/protein affinity ligands are commonly removed from the viral particles and conjugated to protein tracers or nanoparticles for analytical use. This abolishes the advantage of isolating ready-to-use affinity binders and creates the risk of affecting the polypeptide activity. To circumvent this problem, we optimized the phage display system to produce phage particles that express the affinity binder on pIII and a polyglycine short peptide fused to pVIII that allows the covalent attachment of tracer molecules employing sortase A. Using a llama heavy chain only variable domain (VHH) against the herbicide 2,4-D on pIII as the model, we showed that the phage can be extensively decorated with a rhodamine-LPETGG peptide conjugate or the protein nanoluciferase (Nluc) equipped with a C-terminal LPETGG peptide. The maximum labeling amounts of rhodamine-LPETGG and Nluc-LPETGG were 1238 ± 63 and 102 ± 16 per phage, respectively. The Nluc-labeled dual display phage was employed to develop a phage bioluminescent immunoassay (P-BLEIA) for the detection of 2,4-D. The limit of detection and 50% inhibition concentration of P-BLEIA were 0.491 and 2.15 ng mL<sup>-1</sup>, respectively, which represent 16-fold and 8-fold improvement compared to the phage enzyme-linked immunosorbent assay. In addition, the P-BLEIA showed good accuracy for the detection of 2,4-D in spiked samples.
Oligonucleotide Immobilization and Hybridization on Aldehyde-Functionalized Poly(2-hydroxyethyl methacrylate) Brushes (2015)

DNA biosensing requires high oligonucleotide binding capacity interface chemistries that can be tuned to maximize probe presentation as well as hybridization efficiency. This contribution investigates the feasibility of aldehyde-functionalized poly(2-hydroxyethyl methacrylate) (PHEMA) brush-based interfaces for oligonucleotide binding and hybridization. These polymer brushes, which allow covalent immobilization of oligonucleotides, are prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) of HEMA followed by a postpolymerization oxidation step to generate side chain aldehyde groups. A series of polymer brushes covering a range of film thicknesses and grafting densities was investigated with regard to their oligonucleotide binding capacity as well as their ability to support oligonucleotide hybridization. Densely grafted brushes were found to have probe oligonucleotide binding capacities of up to ∼30 pmol/cm(2). Increasing the thickness of these densely grafted brush films, however, resulted in a decrease in the oligonucleotide binding capacity. Less densely grafted brushes possess binding capacities of ∼10 pmol/cm(2), which did not significantly depend on film thickness. The oligonucleotide hybridization efficiencies, however, were highest (93%) on those brushes that present the lowest surface concentration of the probe oligonucleotide. These results highlight the importance of optimizing the probe oligonucleotide surface concentration and binding interface chemistry. The versatility and tunability of the PHEMA-based brushes presented herein makes these films a very attractive platform for the immobilization and hybridization of oligonucleotides.
Needle optical coherence elastography for tissue boundary detection (2012)

We incorporate, for the first time, optical coherence elastography (OCE) into a needle probe and demonstrate its ability to measure the microscopic deformation of soft tissues located well beyond the depth limit of reports to date. Needle OCE utilizes the force imparted by the needle tip as the loading mechanism and measures tissue deformation ahead of the needle during insertion. Measurements were performed in tissue-mimicking phantoms and ex vivo porcine trachea. Results demonstrate differentiation of tissues based on mechanical properties and highlight the potential of needle OCE for in vivo tissue boundary detection.
ON THE PREFERENTIAL INTRA-AGGLOMERATE SINTERING OF CUBIC ZrO_2 CRYSTALLITES (1995)

he preferential intra-agglomerate sintering from 600℃ to 1200℃ of the cubic ZrO_2 crystallites was observed by TEM.When sintering temperatures were≤1000℃,the ZrO_2 crystallites'average diameter was≤100nm.They have an equilibrium morphology of hexagon. The sintering of the crystallites is reached by the coalescence of surfaCes of the same type.After sintered beyond 1100℃,the Zro2 crystallites lose the morphology of hexagon.
Genetic analysis of autoimmune regulator haplotypes in alopecia areata (2008)

Alopecia areata is an immune-mediated disorder, occurring with the highest observed frequency in the rare recessive autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) syndrome caused by mutations of the autoimmune regulator (AIRE) gene on chromosome 21q22.3. We have previously detected association between alopecia areata and a single nucleotide polymorphism (SNP) in the AIRE gene in patients without APECED, and we now report the findings of an extended examination of the association of alopecia areata with haplotype analysis including six SNPs in the AIRE gene: C-103T, C4144G, T5238C, G6528A, T7215C and T11787C. In Caucasian groups of 295 patients and 363 controls, we found strong association between the AIRE 7215C allele and AA [P = 3.8 x 10(-8), OR (95% CI): 2.69 (1.8-4.0)]. The previously reported association between AA and the AIRE 4144G allele was no longer significant on correction for multiple testing. The AIRE haplotypes CCTGCT and CGTGCC showed a highly significant association with AA [P = 6.05 x 10(-6), 9.47 (2.91-30.8) and P = 0.001, 3.51 (1.55-7.95), respectively]. To select the haplotypes most informative for analysis, we tagged the polymorphisms using SNPTag software. Employing AIRE C-103T, G6528A, T7215C and T11787C as tag SNPs, two haplotypes were associated with AA; AIRE CGCT and AIRE CGCC [P = 3.84 x 10(-7), 11.40 (3.53-36.9) and P = 3.94 x 10(-4), 2.13 (1.39-3.24) respectively]. The AIRE risk haplotypes identified in this study potentially account for a major component of the genetic risk of developing alopecia areata.
Single cell RNA sequencing reveals regional heterogeneity of hepatobiliary innate lymphoid cells in a tissue-enriched fashion (2019)

IL-33 promotes type 2 immunity, epithelial repair, and tissue fibrosis by activating group 2 innate lymphoid cells (ILC2). ILC2 lack all known surface markers of mature T, B, NK, and myeloid cell lineages (Linneg), express the IL-33 receptor ST2, and release type 2 cytokines which contribute to cholangiocyte proliferation and activation of hepatic stellate cells. This pathway results in massive proliferation of the extrahepatic bile duct (EHBD) but also exacerbates liver fibrosis, suggesting that there may be tissue-specific subpopulations of IL-33-induced ILC. To determine the tissue-specific subsets of ILC in the hepatobiliary system, we analyzed CD45+Linneg mononuclear cells from IL-33 treated adult Balb/c mouse liver or EHBD by single cell RNA sequencing. Principal component analysis identified 6 major CD45+Linneg cell classes, two of which were restricted to the EHBD. One of these classes, biliary immature myeloid (BIM) cells, was predicted to interact with ILC2 by a network of shared receptor-ligand pairs. BIM highly expressed Gp49 and ST2 receptors on the cell surface while lacking surface expression of markers for mature myeloid cells. In conclusion, single cell RNA sequencing identified IL-33 responsive cell groups regionally confined to the liver or extrahepatic bile duct, including a novel population of CD45+Linneg Gp49-expressing mononuclear cells.
Seismic Response Analysis of a High-Rise-Building with Seismic Isolation Rubber (2019)

Seismic isolation laminated rubber is used as an effective means for suppressing damage to buildings and people in buildings by earthquakes. The restoring-force characteristic of the rubber has non-linerar-vibration character... | Find, read and cite all the research you need on Tech Science Press
Cardiac small-conductance calcium-activated potassium channels in health and disease (2021)

Small-conductance Ca<sup>2+</sup>-activated K<sup>+</sup> (SK, K<sub>Ca</sub>2) channels are encoded by KCNN genes, including KCNN1, 2, and 3. The channels play critical roles in the regulation of cardiac excitability and are gated solely by beat-to-beat changes in intracellular Ca<sup>2+</sup>. The family of SK channels consists of three members with differential sensitivity to apamin. All three isoforms are expressed in human hearts. Studies over the past two decades have provided evidence to substantiate the pivotal roles of SK channels, not only in healthy heart but also with diseases including atrial fibrillation (AF), ventricular arrhythmia, and heart failure (HF). SK channels are prominently expressed in atrial myocytes and pacemaking cells, compared to ventricular cells. However, the channels are significantly upregulated in ventricular myocytes in HF and pulmonary veins in AF models. Interests in cardiac SK channels are further fueled by recent studies suggesting the possible roles of SK channels in human AF. Therefore, SK channel may represent a novel therapeutic target for atrial arrhythmias. Furthermore, SK channel function is significantly altered by human calmodulin (CaM) mutations, linked to life-threatening arrhythmia syndromes. The current review will summarize recent progress in our understanding of cardiac SK channels and the roles of SK channels in the heart in health and disease.
HoaxItaly: a collection of Italian disinformation and fact-checking stories shared on Twitter in 2019 (2020)

We released over 1 million tweets shared during 2019 and containing links to thousands of news articles published on two classes of Italian outlets: (1) disinformation websites, i.e. outlets which have been repeatedly flagged by journalists and fact-checkers for producing low-credibility content such as false news, hoaxes, click-bait, misleading and hyper-partisan stories; (2) fact-checking websites which notably debunk and verify online news and claims. The dataset, which includes also title and body for approximately 37k news articles, is publicly available at https://doi.org/10.7910/DVN/ PGVDHX.
PS-05-002 Penile Prosthesis can Safely be Inserted in Patients with Peyronie’s Disease: Interim Results of The Propper Study (2017)

Assess efficacy, safety, and overall satisfaction of AMS 700 penile prosthesis (PP) placement in men with Peyronie's disease (PD) versus non−Peyronie’s disease (NPD) using the Prospective Registry of Outcomes with Penile Prosthesis for Erectile Restoration (PROPPER). PD is a difficult condition to treat and has had a history of lower patient satisfaction rates in the literature after PP than NPD patients. The PROPPER registry prospectively evaluates outcomes in men undergoing penile prosthesis. Data was collected at baseline and annually out to 5 years. The goal of this analysis was to compare PD patients (n=259) to NPD patients (n=998). 1257 men underwent insertion of AMS 700 at 11 North American sites as of Sep 13, 2016. This is an ongoing study; one and two year data is available on 855 (68.0%) and 619 (49.2%) patients, respectively. When operative times were reported there was a significant difference between PD and NPD patients (52±34 min vs 45±27 min (p=0.001)). PD patients (45.2%) were more likely to receive a CX cylinder compared to NPD patients (35.7%), while NPD patients (53.9%) were more likely to receive a LGX cylinder compared to PD patients (43.6%), p-value: 0.008. There was no significant difference in depression rates between the two groups at baseline, 1 and 2 years follow-up. Overall, 84.1% and 85.1% of patients were satisfied or very satisfied at 1 and 2 year follow-up respectively, with no significant differences in patient satisfaction scores between PD and NPD patients.
An investigation into rational strategies for the design of a near zero energy commercial office building in the United Kingdom (2013)

The United Kingdom government has set the target that all new non-domestic buildings should be ?zero carbon? in operation by 2019. The challenge of implementing this vision of a more energy efficient and less emissions intensive building stock is both technological and organizational in nature. While exemplar buildings have achieved this level of performance in the UK the proliferation of such designs within the building stock at large has not been realised, due to the drive to meet interim emissions targets at the minimum of cost premium. In order to achieve the necessary performance improvement in commercial buildings at large to meet this target a step change in the design methodologies applied to these buildings is required.An extensive review of the literature available to the non-domestic building design team was used to identify four distinct design strategies which aspired to levels of operational efficiency beyond the current standards. These strategies were then developed into methodologies which could be applied to case study buildings. The testing of these methodologies was conducted through the emulation of a dynamic building simulation led design process with the target of optimal operational energy efficiency. The comparison and evaluation of these test case buildings and the design methodologies which were applied to them offered an insight into rational strategies for near zero energy buildings going forward. The robustness of both the final design concepts and the methodologies themselves were tested through a series changes to the external and internal conditions under which the buildings were expected to operate.The result of these investigative measures was a demonstration that the performance improvement of commercial office buildings beyond what is currently typical is possible using a commercial design process if the depth of investigation is sufficient. It also identified significant risks associated with these design methodologies and in particular the response of highly specified building designs to changes in use and local climate.
Disruption of a Guard Cell–Expressed Protein Phosphatase 2A Regulatory Subunit, RCN1, Confers Abscisic Acid Insensitivity in Arabidopsis (2002)

Pharmacological studies have led to a model in which the phytohormone abscisic acid (ABA) may be positively transduced via protein phosphatases of the type 1 (PP1) or type 2A (PP2A) families. However, pharmacological evidence also exists that PP1s or PP2As may function as negative regulators of ABA signaling. Furthermore, recessive disruption mutants in protein phosphatases that function in ABA signal transduction have not yet been identified. A guard cell-expressed PP2A gene, RCN1, which had been characterized previously as a molecular component affecting auxin transport and gravity response, was isolated. A T-DNA disruption mutation in RCN1 confers recessive ABA insensitivity to Arabidopsis. The rcn1 mutation impairs ABA-induced stomatal closing and ABA activation of slow anion channels. Calcium imaging analyses show a reduced sensitivity of ABA-induced cytosolic calcium increases in rcn1, whereas mechanisms downstream of cytosolic calcium increases show wild-type responses, suggesting that RCN1 functions in ABA signal transduction upstream of cytosolic Ca(2+) increases. Furthermore, rcn1 shows ABA insensitivity in ABA inhibition of seed germination and ABA-induced gene expression. The PP1 and PP2A inhibitor okadaic acid phenocopies the rcn1 phenotype in wild-type plants both in ABA-induced cytosolic calcium increases and in seed germination, and the wild-type RCN1 genomic DNA complements rcn1 phenotypes. These data show that RCN1 functions as a general positive transducer of early ABA signaling.
ChemInform Abstract: What Can We Learn About Battery Materials from Their Magnetic Properties? (2011)

Abstract Review: 91 refs.
Review on Dynamic Contour Tonometry and Ocular Pulse Amplitude (2015)

Intraocular pressure (IOP) measurement is the cornerstone of the management of glaucoma patients. The gold standard for assessing IOP is Goldmann applanation tonometry (GAT). Recently, the dynamic contour tonometer (DCT) has become available. While both devices provide reliable IOP measurements, the results are not interchangeable. DCT has the advantage of measuring an additional parameter: ocular pulse amplitude (OPA). OPA is defined as the difference between systolic and diastolic IOP and represents the pulsatile wave front produced by the varying amount of blood in the eye during the cardiac cycle. It has been shown to vary with ocular structural parameters, such as axial length, corneal thickness, and ocular rigidity, as well as with systemic variables like heart rate, blood pressure, and left ventricular ejection fraction. Although the existence of some of these associations is still controversial, the clinical relevance of OPA has been consistently suggested, especially in glaucoma. Further research on this intriguing parameter could not only provide insight into glaucoma pathophysiology but also help integrate this variable into clinical practice.
Pyroelectricity of biomaterials (1984)

Abstract Pyroelectricity has been observed in animal tissues as diverse as those of amphibians and mammals, and in plant tissues ranging from algae to trees. Its presence in sensory organs and the existence of biological ferroelectricity have also been reported. Extensive reviews, which summarize the available data and present many possible physiological implications, have been published.1,2 Although present knowledge is fragmentary and largely qualitative, it is likely that pyroelectricity is an essential and universal property of all biological systems.
En el Centenario del Colegio Regional del Centro de Árbitros; después Colegio Castellano y Comité Madrileño. (2014)

En el Centenario del Colegio Regional del Centro de Arbitros; despues Colegio Castellano y Comite Madrileno. Autor: Luis Javier Bravo Mayor Cuadernos de futbol, no 53, abril 2014. ISSN: 1989-6379 Fecha de recepcion: 05-03-2014, Fecha de aceptacion: 17-03-2014. URL: http://www.cihefe.es/cuadernosdefutbol/2014/04/en-el-centenario-del-colegio-regional-delcentro-de-arbitros-despues-colegio-castellano-y-comite-madrileno/ Resumen
TravInfo Field Operational Test Evaluation: Information Service Providers Customer Survey (2000)

This paper presents the findings of the customer study of Information Service Providers. TheInformation Service Provider customer study is part of the traveler response evaluation for theTravInfo Field Operational Test. Presently, three private partners of the TravInfo project deploytraffic Web sites using exclusively the TravInfo database. A survey of Web site users wasconducted over seven months from August 1998 – March 1999. A survey instrument wasincorporated into the Web site. The study addressed the usage of Web site information and thetravel behavior of Web site users. The key finding of the survey is that Web site service maysignificantly influence travel behavior.&amp;nbsp; The vast majority of those who retrieved incident reportsmodified their trips.&amp;nbsp; The study showed that drivers who encounter traffic congestion frequentlyare likely to access information on a regular basis. Most traffic Web site users are repeatcustomers because they are satisfied with the information they have obtained through theInternet. People who retrieve Web site information have more travel options than those whoobtain information from other sources. Web site information is more understandable than theinformation disseminated through other media.&amp;nbsp; People perceived that the quality of Web siteinformation is better than other sources in terms of its usefulness and reliability. Web site usersare more receptive to traffic reports than are those who use other information sources. Keywords:traffic Web site, travel behavior.
An improved phenanthrene synthesis: a simple route to (±)-tylophorine (1977)

A. J. Liepa and R. E. Summons, J. Chem. Soc., Chem. Commun., 1977, 826 DOI: 10.1039/C39770000826
Association of Circulating Caprylic Acid with Risk of Mild Cognitive Impairment and Alzheimer's Disease in the Alzheimer's Disease Neuroimaging Initiative (ADNI) Cohort (2023)

The inverse associations were present only among subgroups of CN participants, including female individuals, those with one or more cardiometabolic diseases, or one APOE ε4 allele, or higher ADAS-Cog 13 scores. If confirmed, this finding will facilitate precision prevention of MCI, in turn, AD among CN older adults.
Preterm Infants With Congenital Heart Disease and Bronchopulmonary Dysplasia: Postoperative Course and Outcome After Cardiac Surgery (2005)

BPD has significant implications for children who have CHD and undergo cardiac surgery, leading to prolonged ICU and hospital stays, although most survivors are not O2 dependent. Postoperative mortality was highest among patients with univentricular hearts and severe BPD. Optimal timing of surgery and strategies to improve outcome remains to be delineated.