gebhart/patrepeval huggingface dataset:
Multidimensional Fractionation Is a Requirement for Quantitation of Golgi-Resident Glycosylation Enzymes from Cultured Human Cells (2014)

Glycosylation results from the concerted action of glycosylation enzymes in the secretory pathway. In general, gene expression serves as the primary control mechanism, but post-translational fine-tuning of glycosylation enzyme functions is often necessary for efficient synthesis of specific glycan epitopes. While the field of glycomics has rapidly advanced, there lacks routine proteomic methods to measure expression of specific glycosylation enzymes needed to fill the gap between mRNA expression and the glycomic profile in a "reverse genomics" workflow. Toward developing this workflow we enriched Golgi membranes from two human colon cancer cell lines by sucrose density centrifugation and further mass-based fractionation by SDS-PAGE. We then applied mass spectrometry to demonstrate a doubling in the number of Golgi resident proteins identified, compared to the unenriched, low speed centrifuged supernatant of lysed cells. A total of 35 Golgi-resident glycosylation enzymes, of which 23 were glycosyltransferases, were identified making this the largest protein database so far of Golgi resident glycosylation enzymes experimentally identified in cultured human cells. We developed targeted mass spectrometry assays for specific quantitation of many of these glycosylation enzymes. Our results show that alterations in abundance of glycosylation enzymes at the protein level were generally consistent with the resultant glycomic profiles, but not necessarily with the corresponding glycosyltransferase mRNA expression as exemplified by the case of O-glycan core 1 T synthase.
Impacts of Balancing Valves on Pump Energy and Control Performance in Variable Water Flow Systems (2005)

There is no common agreement on the use of balance valves in the most popular variable water flow systems. A comprehensive theoretical analysis has been conducted to investigate the impacts of balance valves on initial cost, pump operation power, and system control performance. The investigation finds that eliminating balance valves can (i) reduce by 20–40% pump energy consumption in a typical variable flow water system, (ii) reduce the maximum pressure drop across control valves, and (iii) improve pump efficiency at most partial load conditions. The reduced pressure drops across control valves improve system control performance and may allow selecting some control valves at lower close-off pressures. Consequently, eliminating balancing valves can also potentially reduce system initial cost.
Spatial Application of Southern U.S. Pine Water Yield for Prioritizing Forest Management Activities (2023)

Forest management depends on forest condition data and the ability to quantify the impacts of management activities to make informed decisions. Spatially quantifying water yield (WY) from forests across large landscapes enables managers to consider potential WY changes when designing forest management plans. Current forest water yield datasets are either spatially coarse or too restricted to specific sites with in situ monitoring to support some project-level forest management decisions. In this study, we spatially apply a stand-level southern pine WY model over a forested landscape in the Florida panhandle. We informed the WY model with pine leaf area index inputs created from lidar remote sensing and field data, a spatial and temporal aridity index from PRISM and MODIS data, and a custom depth to groundwater dataset. Baseline WY conditions for the study area were created using the Esri and Python tools we developed to automate the WY workflow. Several timber thinning scenarios were then used to quantify water yield increases from forest management activities. The results of this methodology are detailed (10 m spatial resolution) forest WY raster datasets that are currently being integrated with other spatial datasets to inform forest management decisions.
Accuracy characterization for metropolitan-scale Wi-Fi localization (2005)

Location systems have long been identified as an important component of emerging mobile applications. Most research on location systems has focused on precise location in indoor environments. However, many location applications (for example, location-aware web search) become interesting only when the underlying location system is available ubiquitously and is not limited to a single office environment. Unfortunately, the installation and calibration overhead involved for most of the existing research systems is too prohibitive to imagine deploying them across, say, an entire city. In this work, we evaluate the feasibility of building a wide-area 802.11 Wi-Fi-based positioning system. We compare a suite of wireless-radio-based positioning algorithms to understand how they can be adapted for such ubiquitous deployment with minimal calibration. In particular, we study the impact of this limited calibration on the accuracy of the positioning algorithms. Our experiments show that we can estimate a user's position with a median positioning error of 13-40 meters (depending upon the characteristics of the environment). Although this accuracy is lower than existing positioning systems, it requires substantially lower calibration overhead and provides easy deployment and coverage across large metropolitan areas.
Survival of Multiple Myeloma Patients Aged 65-70 Years in the Era of Novel Agents and Autologous Stem Cell Transplantation (2014)

Novel agents such as thalidomide, lenalidomide and bortezomib have dramatically changed the treatment paradigm of multiple myeloma (MM). However, it is not clear whether these agents improve the prognosis of elderly patients who have undergone autologous stem cell transplantation (auto-SCT). We retrospectively analyzed the outcome of 318 newly diagnosed patients aged 65–70 years who were treated between January 1, 2004, and December 31, 2009. As initial therapy, 192 patients were treated with conventional chemotherapy,88 with novel agent-containing regimens, 21 with conventional chemotherapy plus auto-SCT and the remaining 17 with novel agents plus auto-SCT. The median progression-free survival was 19.1, 24.5, 26.8 and 35.2 months, respectively, and the 5-year overall survival (OS) was 40, 62, 63 and 87%, respectively. Initial therapy with novel agents (p < 0.001) or auto-SCT (p < 0.02) significantly improved OS compared with the group without these treatment modalities. Salvage therapy with novel agents also significantly improved survival after relapse compared with conventional chemotherapy alone (p < 0.04). In a multivariate analysis, the use of novel agents was an independent prognostic factor significantly associated with extended OS(p < 0.003). These results indicate that novel agents and auto-SCT had a major impact on OS in eligible patients in this subgroup of MM.
Two Anthracene-Based Copolymers as the Hole-Transporting Materials for High-Performance Inverted (p-i-n) Perovskite Solar Cells (2018)

Two anthracene-based copolymers, the thiophene-bridged carbazole-anthracene copolymer (abbreviated as PCBZANT) and the thiophene-bridged triphenylamine-anthracene copolymer (abbreviated as PTPAANT), have been developed as the hole-transporting materials (HTMs) for the inverted perovskite solar cells. They were thermally stable with decomposition temperatures of 435 and 420 °C. The High Occupied Molecular Orbitals (HOMO) of −5.15 and −5.24 eV of two copolymers facilitated the hole carriers transfer from the perovskite layer (CH3NH3PbI3, HOMO: −5.4 eV) in contrast to poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS, HOMO: −4.9 eV). The solar cell with PCBZANT (abbreviated as the PCBZANT device) showed the highest power conversion efficiency (PCE) of 15.50%, while the cell with PTPAANT (abbreviated as the PTPAANT device) showed the highest PCE of 14.52%, with increases of 36.2% and 27.6%, respectively, relative to the PEDOT:PSS device. The thorough analysis disclosed that the high performance was mainly ascribed to the enhanced open-circuit voltage (VOC) and short-circuit current density (JSC), being contributed from the efficient hole-carrier extraction, the high hole mobility of two copolymers, and the high-quality perovskite film with large crystal size and less defect. With strong absorption in the range of 350–500 nm, the polymers decreased the destruction of UV-radiation on the perovskite layer as UV-filters and improved the stability of the inverted cells.
Short-term Optic Disc Cupping Reversal in a Patient With Mild Juvenile Open-angle Glaucoma Due to Early Idiopathic Intracranial Hypertension (2018)

An unexplained reduction of optic nerve cup-to-disc ratio should prompt a workup for other etiologies, such as increased intracranial pressure. Baseline photographs not subjected to computerized scan obsolescence are extremely useful in monitoring the long-term appearance of asymmetric optic discs as an adjunct to the clinical examination.
Liquid-Liquid Equilibria for Ternary Mixtures Containing Vegetable Oils, Methanol, and Cosolvents (2010)

The liquid-liquid equilibrium (LLE) phase boundaries were determined experimentally for the ternary systems containing refined sunflower oil, methanol, and one of ten potential cosolvents at 308.2 K under atmospheric pressure by using cloud point method. n-Butylamine was found to be one of the best cosolvents, which could substantially enhance the miscibility between the oil and methanol. The LLE measurements were then extended to the ternary systems of methanol + refined sunflower oil, soybean oil, or canola oil in the presence of the auxiliary cosolvent n-butylamine at temperatures from 298.2 K to 318.2 K. The LLE data were utilized for estimating the model parameters of the NRTL and the UNIQUAC, respectively. In general, these two models can reasonably represent the LLE phase boundaries.
Effect of meconium on the surface properties of perflubron* (2004)

These data are consistent with reported observations of increased lung compliance in perfluorocarbon-treated animals with meconium aspiration. In addition to these clinical implications, the novel interfacial tension technique described here could prove useful for assessing the interfacial properties of other poorly miscible biological fluids.
Luminescence of Sr[sub 2]SiO[sub 4−x]N[sub 2x∕3]:Eu[sup 2+] Phosphors Prepared by Spark Plasma Sintering (2007)

Spark plasma sintering (SPS) was employed to synthesize , , and for use in white light emitting diodes (LED) lightings. The SPS technique enabled a complete, rapid synthesis of these phosphors with ease, whereas it is difficult to produce nitridosilicate phosphors by any other conventional synthesis methods. The photoluminescent (PL) spectrum of had two emission peaks, one at 420 and the other at . There existed a single peak at in the case of . The PL spectrum of also had two peaks, one at 529 and the other at . Even though the exact structure was not identified, it was revealed that the X-ray diffraction pattern of , , and was consistent with the PL data. The phosphor with the highest nitrogen content, , showed a broad emission band spanning almost the entire visible range, which may work best if coupled with either UV or blue LEDs.
Dual Effects of Ethylene on Potato Dormancy and Sprout Growth (1974)

Dormant potato tubers (Solanum tuberosum L.) of two cultivars were treated with various concentrations of ethylene gas for various exposure periods. As has been shown by others, ethylene caused a rapid but transient increase in respiration rate, which appeared to be independent of any effects on dormancy. All concentrations tested caused accelerated sprouting, 2 microliters per liter being the most effective. Ethylene exerts a dual effect on potato tubers: it markedly shortens the duration of rest, but it inhibits elongation of the sprouts during extended treatment. Comparing these results with published work on seeds, bulbs, and corms suggests that ethylene must have a significant but as yet unexplained role in rest and dormancy. However, since the most effective ethylene treatment did not equal the response elicited by treatment with ethylene chlorhydrin, other factors must also contribute to termination of rest.
Renal Function–Based Contrast Threshold Predicts Kidney Injury in Transcatheter Aortic Valve Replacement (2022)

CV in the context of renal function administrated during TAVR is a robust tool to predict AKI. AKI after TAVR is associated with an increased risk of mortality. Incorporation of thresholds of >2× and > 3× eGFR into procedural planning should be considered as a quality initiative.
Risk Factors for Postoperative Retropharyngeal Hematoma After Anterior Cervical Spine Surgery (2013)

In Brief Study Design. Retrospective review of prospective database. Objective. To investigate risk factors involved in the development of anterior cervical hematomas and determine any impact on patient outcomes. Summary of Background Data. Postoperative (PO) hematomas after anterior cervical spine surgery require urgent recognition and treatment to avoid catastrophic patient morbidity or death. Current studies of PO hematomas are limited. Methods. Cervical spine surgical procedures performed on adults by the senior author at a single academic institution from 1995 to 2012 were evaluated. Demographic data, surgical history, operative data, complications, and neck disability index (NDI) scores were recorded prospectively. Cases complicated by PO hematoma were reviewed, and time until hematoma development and surgical evacuation were determined. Patients who developed a hematoma (HT group) were compared with those that did not (no-HT group) to identify risk factors. NDI outcomes were compared at early (<11 mo) and late (>11 mo) time points. Results. There were 2375 anterior cervical spine surgical procedures performed with 17 occurrences (0.7%) of PO hematoma. In 11 patients (65%) the hematoma occurred within 24 hours PO, whereas 6 patients (35%) presented at an average of 6 days postoperatively. All underwent hematoma evacuation, with 2 patients (12%) requiring emergent cricothyroidotomy. Risk factors for hematoma were found to be (1) the presence of diffuse idiopathic skeletal hyperostosis (relative risk = 13.2, 95% confidence interval = 3.2–54.4), (2) presence of ossification of the posterior longitudinal ligament (relative risk = 6.8, 95% confidence interval = 2.3–20.6), (3) therapeutic heparin use (relative risk 148.8, 95% confidence interval = 91.3–242.5), (4) longer operative time, and (5) greater number of surgical levels. The occurrence of a PO hematoma was not found to have a significant impact on either early (HT: 30, no-HT: 28; P = 0.86) or late average NDI scores (HT: 28, no-HT 31; P = 0.76). Conclusion. With fast recognition and treatment, no long-term detriment from PO anterior cervical hematoma was found. We identified risk factors to be (1) presence of diffuse idiopathic skeletal hyperostosis, (2) presence of ossification of the posterior longitudinal ligament, (3) therapeutic heparin use, (4) longer operative time, and (5) greater number of surgical levels. Level of Evidence: 4 A review of 2375 anterior cervical surgical procedures revealed 17 occurrences (0.7%) of postoperative (PO) hematoma. Identified risk factors were (1) diffuse idiopathic skeletal hyperostosis, (2) ossification of the posterior longitudinal ligament, (3) therapeutic heparin use, (4) longer operative time, and (5) greater number of surgical levels. When appropriately treated, the occurrence of a PO hematoma was not found to impact neck disability index outcome scores.
A Family of Potato Genes That Encode Kunitz-Type Proteinase Inhibitors: Structural Comparisons and Differential Expression (1994)

Potato tubers contain a complex group of proteins of 20 to 24 kDa that exhibit homology to Kunitz-type proteinase inhibitors. We isolated three cDNAs and two genomic clones that encode members of the potato Kunitz-type proteinase inhibitor (PKPI) family. Comparison of the structures of these and other cloned genes indicated that genes of the PKPI family can be classified into three major homology groups, namely, A, B and C. The PKPI-A and -B genes exhibit higher homology to one another than to the PKPI-C genes. Determination of the N-terminal amino acid sequences of 18 polypeptides from the complex group of 20- to 24-kDa proteins that had been separated by column chromatography and subsequently gel electrophoresis revealed three different sequences that corresponded to PKPI-A, -B, and -C. PKPI-A genes include those coding for a cathepsin D inhibitor, while PKPI-B and -C genes include those coding for trypsin and/or chymotrypsin inhibitors and a subtilisin inhibitor. Precursors to PKPIs are synthesized with an N-terminal extra peptide that appears to contain, in addition to the signal peptide, a short propeptide with a highly conserved Asn-Pro-Ile-Xxx-Leu-Pro motif that is identical to the potential vacuolar-sorting determinant in the N-terminal propeptide of a precursor to sporamin of sweet potato. Expression of the PKPI-A and -B genes is differentially regulated: PKPI-A mRNA but not PKPI-B mRNA were induced in leaves after wounding or upon treatment with methyl jasmonate. Nuclear genes for PKPI-A and -B do not contain introns, and the homology between the two types of gene extends only 72 bp upstream from the site of initiation of transcription.
Photochemical Rearrangement of N-Mesyloxylactams: Stereospecific Formation of N-Heterocycles (2010)

N-Mesyloxylactams undergo an efficient ring-contraction to N-heterocycles of various ring sizes. Yields increase with the degree of substitution α to the carbonyl. The stereochemical information of a chiral migrating carbon is conserved making this reaction a synthetically useful complement to the well-known Hofmann, Curtius, Lossen, and Schmidt rearrangements.
Polyvinyl Isobutyll Ethers (1948)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPolyvinyl Isobutyll EthersC. E. Schildknecht, S. T. Gross, H. R. Davidson, J. M. Lambert, and A. O. ZossCite this: Ind. Eng. Chem. 1948, 40, 11, 2104–2115Publication Date (Print):November 1, 1948Publication History Published online1 May 2002Published inissue 1 November 1948https://pubs.acs.org/doi/10.1021/ie50467a021https://doi.org/10.1021/ie50467a021research-articleACS PublicationsRequest reuse permissionsArticle Views454Altmetric-Citations142LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Finite element model updating with quantified uncertainties using point cloud data (2023)

Abstract While finite element (FE) modeling is widely used for ultimate strength assessments of structural systems, incorporating complex distortions and imperfections into FE models remains a challenge. Conventional methods typically rely on assumptions about the periodicity of distortions through spectral or modal methods. However, these approaches are not viable under the many realistic scenarios where these assumptions are invalid. Research efforts have consistently demonstrated the ability of point cloud data, generated through laser scanning or photogrammetry-based methods, to accurately capture structural deformations at the millimeter scale. This enables the updating of numerical models to capture the exact structural configuration and initial imperfections without the need for unrealistic assumptions. This research article investigates the use of point cloud data for updating the initial distortions in a FE model of a stiffened ship deck panel, for the purposes of ultimate strength estimation. The presented approach has the additional benefit of being able to explicitly account for measurement uncertainty in the analysis. Calculations using the updated FE models are compared against ground truth test data as well as FE models updated using standard spectral methods. The results demonstrate strength estimation that is comparable to existing approaches, with the additional advantages of uncertainty quantification and applicability to a wider range of application scenarios.
Understanding Drivers’ Stress and Interactions With Vehicle Systems Through Naturalistic Data Analysis (2021)

Today, and probably for a long time to come, humans will remain an integral part of vehicles for driving tasks. Therefore, it is essential to understand how vehicles and drivers interact with each other and how drivers’ behavior and physical and mental states affect vehicle performance and traffic safety. This article explores the relationship between driver and vehicle in real-world driving conditions by analyzing large-scale naturalistic data collected from cars and drivers. Specifically, more than 800 hours of driving data from 16 drivers were collected using three different data sources (telematics data, video frames, and physiological data) and two types of analysis were done. The first one analyzes different types of driver-vehicle interactions during driving. The second one investigates the effect of different driving conditions on drivers’ stress and explores the relationship between driver and vehicle in different driving conditions. Our experimental results show that drivers’ physiological signals are correlated with some variables from vehicle kinematics and influenced by drivers’ behavior inside the vehicle. These findings could be used to help manage comfort-related in-vehicle intervention systems and could provide a continuous measure of how different external conditions (traffic, road, weather, etc.) affect drivers.
Two-Phase Flow-Induced Vibration of Parallel Triangular Tube Arrays With Asymmetric Support Stiffness (2009)

Laboratory experiments were conducted to determine the flow-induced vibration response and fluidelastic instability threshold of model heat exchanger tube bundles subjected to a cross-flow of refrigerant 11. Tube bundles were specially built with tubes cantilever-mounted on rectangular brass support bars so that the stiffness in the streamwise direction was about double that in the transverse direction. This was designed to simulate the tube dynamics in the U-bend region of a recirculating-type nuclear steam generator. Three model tube bundles were studied, one with a pitch ratio of 1.49 and two with a smaller pitch ratio of 1.33. The primary intent of the research was to improve our understanding of the flow-induced vibrations of heat exchanger tube arrays subjected to two-phase cross-flow. Of particular concern was to compare the effect of the asymmetric stiffness on the fluidelastic stability threshold with that of axisymmetric stiffness arrays tested most prominently in literature. The experimental results are analyzed and compared with existing data from literature using various definitions of two-phase fluid parameters. The fluidelastic stability thresholds of the present study agree well with results from previous studies for single-phase flow. In two-phase flow, the comparison of the stability data depends on the definition of two-phase flow velocity.