gebhart/patrepeval huggingface dataset:
S‐layer protein from Thermus thermophilus HB8 assembles into porin‐like structures (1993)

The cells of the extreme thermophile Thermus thermophilus are surrounded by a regular layer (S-layer) built up by a protein with an apparent molecular mass of 100 kDa (P100). From purified membrane fractions, three different class of two-dimensional crystals can be obtained by following alternative extractive procedures. One of these crystals, with p6 symmetry, clearly represents the native S-layer detected by freeze etching on whole cells, while the other two, showing p2 and p3 symmetries respectively, closely resemble aggregates of bacterial porins. We demonstrate here by limited proteolysis and Western blotting the surprising fact that the protein component of the three crystals is the P100 protein. Our biochemical data also show how this protein forms Ca(2+)-stabilized trimers in each crystal, which support the structural analysis that points to p3 units as the common structural block in all of them, and again resembles the situation found in bacterial porins.
Chemical Studies on the Annonaceae – VI. The Alkaloids of the Stem and Fruit of Monanthotaxis cauliflora1 (1979)

Alkaloids have been isolated, in trace amounts only, from the whole stem and mature fruits of Monanthotaxis (Popowia) cauliflora (Chipp) Verdc. From the . stem four alkaloids, three aporphines and a tetrahydroprotoberberine, were obtained. Two of the three aporphines were identified as nuciferine and asimilobine and evidence is presented that the third is the apparently novel 9–hydroxy–1,2– dimethoxy–noraporphine. The tetrahydroprotoberberine was identified as stepholidine. The two alkaloids from the fruit were partially identified as a 1,2–substituted–9–methoxy–7–hydroxyaporphine and a dihydroxy–dimethoxy–noraporphine, probably laurelliptine.
A 4-in-1 (WiFi/BT/FM/GPS) connectivity SoC with enhanced co-existence performance in 65nm CMOS (2012)

In recent years, the increasing popularity of mobile devices, such as smart- phones and tablets, is driving the demand for integrating multiple radios on a single SoC to reduce cost, form factor and external BOM. These devices require ubiquitous wireless connectivity, which means concurrent operation with different radios. While concurrent operation of multiple radios brings excellent user experiences, there exist great challenges in dealing with radio co-existence in an SoC. For example, concurrent operation between WiFi and BT, both oper- ating in the 2.4GHz ISM band, sets additional requirements in RF front-end cir- cuits and system control. In addition, thermal effect of the integrated WiFi PA needs to be compensated to minimize its impact on the frequency-precise GPS system.
Low‐Temperature Internal Sterilization of Medical Plastic Tubes Using a Linear Dielectric Barrier Discharge (2008)

Abstract The use of a linear DBD for the internal sterilization of thin plastic tubes used in medical facilities is studied. We demonstrated that flexible, long, linear DBD plasmas were produced with a diameter of 0.2–3 mm and a length longer than 1 m. The internal sterilization experiments of medical plastic tubes were carried out using a thin, linear DBD. We confirmed that 10 6 Geobacillus stearothermophilus spores were killed inside the tube at room temperature after 12 min DBD irradiation under atmospheric conditions. The sterilization was attributed to UV radiation of the nitrogen molecules' second positive system, the ozone generated in the air, and/or their synergetic effect. magnified image
A Comparative Study of Multiple Approaches for Predicting the Soil-Water Retention Curve: Hyperspectral Information vs. Basic Soil Properties (2015)

Information about the soil–water retention curve is necessary for modeling water flow and solute transport processes in soils. Soil spectroscopy in the visible, near-infrared, and shortwave infrared (Vis-NIR-SWIR) range has been widely used as a rapid, cost-effective and nondestructive technique to predict soil properties. However, less attention has been paid to predict soil hydraulic properties using soil spectral data. In this paper, spectral reflectances of soil samples from the Zanjanrood watershed, Iran, were measured in the Vis-NIR-SWIR ranges (350–2500 nm). Stepwise multiple linear regression coupled with the bootstrap method was used to construct predictive models and to estimate the soil–water retention curve. We developed point and parametric transfer functions based on the van Genuchten (VG) and Brooks-Corey (BC) soil hydraulic models. Three different types of transfer functions were developed: (i) spectral transfer functions (STFs) that relate VG/BC hydraulic parameters to spectral reflectance values, (ii) pedotransfer function (PTFs) that use basic soil data as input, and (iii) PTFs that consider spectral data and basic soil properties, further referred to as spectral pedotransfer functions (SPTFs). We also derived and evaluated point transfer functions which estimate soil–water contents at specific matric potentials. The point STFs and SPTFs were found to be accurate at low and intermediate water contents (R2 > 0.50 and root mean squared error [RMSE] < 0.018 cm3 cm−3), while the point PTFs performed better close to saturation. The parametric STFs and SPTFs of both the VG and BC models performed similarly to parametric PTFs in estimating the retention curve. The best predictions of soil–water contents were obtained for all the three transfer functions when the VG and BC retention models were fitted to the retention points estimated by the point transfer functions. Overall, our findings indicate that spectral data can provide useful information to predict soil—water contents and the soil–water retention curve. However, there is a need to extend and validate the derived transfer functions to other soils and regions.
Metal ion levels following resurfacing arthroplasty of the hip (2010)

We report serum metal ion level data in patients with unilateral and bilateral hip resurfacing over a ten-year period. In these patients there is an increase in both cobalt and chromium levels above the accepted reference ranges during the first 18 months after operation. Metal ion levels remain elevated, but decline slowly for up to five years. However, the levels then appear to start rising again in some patients up to the ten-year mark. There was no significant difference in cobalt or chromium levels between men and women. These findings appear to differ from much of the current literature. The clinical significance of a raised metal ion level remains under investigation.
Issues with measurements of the convective velocity of large-scale structures in the compressible shear layer of a free jet (2008)

Experiments were conducted to measure convective velocity of large-scale structures in the shear layer of a Mach 2.0 free jet with a convective Mach number of 0.87 and Reynolds number based on a nozzle diameter of 2.6×106. Real-time flow visualization and planar Doppler velocimetry (PDV) measurements along with space-time correlation were used. The results reveal that regardless of the measurement technique employed, whether qualitative flow visualization or quantitative PDV, the method of seeding can have a significant effect on the ability to accurately identify, track, and measure convective velocity of large-scale structures. This is apparent by the relatively large spread in convective velocity results obtained in the same jet by using various methods of seeding and qualitative and quantitative visualization. Convective velocity measurements made under the best seeding conditions available with PDV indicate a convective velocity of structures that is close to the value predicted by theory up to the convective Mach number of 0.87 used in the current work. This is in contrast to previously reported convective velocity measurements (including those of the present authors) that indicated a deviation from the theoretically predicted value but were based on measurements made under less than ideal seeding conditions.
LSD1: A single target to combat lineage plasticity in lethal prostate cancer (2018)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Low-Cost Readout Electronics for Piezoresistive MEMS-Based Transducers (2019)

The design, fabrication and testing of low-cost readout electronics for piezoresistive MEMS-based transducers are presented in this paper. The proposed circuit architecture has a low input-referred noise power spectral density of 60nV/√Hz, a latency of 2.52μs and provides offset compensation with a resolution of 72μV for piezoresistive MEMS-based transducers such as microcantilevers and micromembranes. Measurement results performed with a microcantilever used for force measurements and a micromembrane used for pressure measurements show the excellent performance of the proposed readout electronics for a frequency range from 10Hz to 9.6kHz.
Is Increased Intracellular Calcium in Red Blood Cells a Common Component in the Molecular Mechanism Causing Anemia? (2017)

For many hereditary disorders, although the underlying genetic mutation may be known, the molecular mechanism leading to hemolytic anemia is still unclear and needs further investigation. Previous studies revealed an increased intracellular Ca<sup>2+</sup> in red blood cells (RBCs) from patients with sickle cell disease, thalassemia, or Gardos channelopathy. Therefore we analyzed RBCs' Ca<sup>2+</sup> content from 35 patients with different types of anemia (16 patients with hereditary spherocytosis, 11 patients with hereditary xerocytosis, 5 patients with enzymopathies, and 3 patients with hemolytic anemia of unknown cause). Intracellular Ca<sup>2+</sup> in RBCs was measured by fluorescence microscopy using the fluorescent Ca<sup>2+</sup> indicator Fluo-4 and subsequent single cell analysis. We found that in RBCs from patients with hereditary spherocytosis and hereditary xerocytosis the intracellular Ca<sup>2+</sup> levels were significantly increased compared to healthy control samples. For enzymopathies and hemolytic anemia of unknown cause the intracellular Ca<sup>2+</sup> levels in RBCs were not significantly different. These results lead us to the hypothesis that increased Ca<sup>2+</sup> levels in RBCs are a shared component in the mechanism causing an accelerated clearance of RBCs from the blood stream in channelopathies such as hereditary xerocytosis and in diseases involving defects of cytoskeletal components like hereditary spherocytosis. Future drug developments should benefit from targeting Ca<sup>2+</sup> entry mediating molecular players leading to better therapies for patients.
Controllable Engineering and Functionalizing of Nanoparticles for Targeting Specific Proteins towards Biomedical Applications (2021)

Nanoparticles have been widely used in important biomedical applications such as imaging, drug delivery, and disease therapy, in which targeting toward specific proteins is often essential. However, current targeting strategies mainly rely on surface modification with bioligands, which not only often fail to provide desired properties but also remain challenging. Here an unprecedented approach is reported, called reverse microemulsion-confined epitope-oriented surface imprinting and cladding (ROSIC), for facile, versatile, and controllable engineering coreless and core/shell nanoparticles with tunable monodispersed size as well as specific targeting capability toward proteins and peptides. Via engineering coreless imprinted and cladded silica nanoparticles, the effectiveness and superiority over conventional imprinting of the proposed approach are first verified. The prepared nanoparticles exhibit both high specificity and high affinity. Using quantum dots, superparamagnetic nanoparticles, silver nanoparticles, and upconverting nanoparticles as a representative set of core substrates, a variety of imprinted and cladded single-core/shell nanoparticles are then successfully prepared. Finally, using imprinted and cladded fluorescent nanoparticles as probes, in vitro targeted imaging of triple-negative breast cancer (TNBC) cells and in vivo targeted imaging of TNBC-bearing mice are achieved. This approach opens a new avenue to engineering of nanoparticles for targeting specific proteins, holding great prospects in biomedical applications.
BLADDER TUMOR MARKERS, INTRAVESICAL THERAPY AND SYSTEMIC CHEMOTHERAPY (2001)

No AccessJournal of UrologyCLINICAL UROLOGY: Original Articles: Editorial1 Aug 2001BLADDER TUMOR MARKERS, INTRAVESICAL THERAPY AND SYSTEMIC CHEMOTHERAPY Mark S. Soloway Mark S. SolowayMark S. Soloway More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65968-XAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "BLADDER TUMOR MARKERS, INTRAVESICAL THERAPY AND SYSTEMIC CHEMOTHERAPY." The Journal of Urology, 166(2), pp. 488–489 References 1 : Transitional cell carcinoma of the prostate. J Urol1991; 146: 1207. Link, Google Scholar 2 : Current bladder tumor tests: does their projected utility fulfill clinical necessity?. J Urol2001; 165: 1067. Link, Google Scholar 3 : De novo muscle invasive bladder cancer: is there a change in trend?. J Urol2001; 165: 47. Link, Google Scholar 4 : Effect of chemotherapeutic agents on bladder cancer: a new animal model. Surg Forum1973; 24: 542. Google Scholar 5 : Single and combination chemotherapy for primary murine bladder cancer. Cancer1975; 36: 333. Google Scholar 6 : Cis-dichlorodiammineplatinum(II) in advanced bladder cancer. Cancer Treat Rep1976; 60: 917. Google Scholar 7 : Preliminary results of M-VAC (methotrexate, vinblastine, doxorubicin and cisplatin) for transitional cell carcinoma of the urothelium. J Urol1985; 133: 403. Link, Google Scholar 8 : Gemcitabine and cisplatin versus methotrexate, vinblastine, doxorubicin, and cisplatin in advanced or metastatic bladder cancer: results of a large, randomized, multinational, multicenter, phase III study. J Clin Oncol2000; 18: 3068. Google Scholar 9 : Phase II trial of gemcitabine plus cisplatin in patients with metastatic urothelial cancer. J Clin Oncol2000; 18: 1921. Google Scholar Department of Urology, University of Miami School of Medicine, Miami, Florida© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 166Issue 2August 2001Page: 488-489 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.MetricsAuthor Information Mark S. Soloway More articles by this author Expand All Advertisement PDF downloadLoading ...
Probucol Promotes Functional Reendothelialization in Balloon-Injured Rabbit Aortas (2003)

Probucol inhibits intimal thickening in balloon-damaged arteries of rabbits by promoting the regeneration of functional endothelium, without affecting the proportion of aortic lipids that was oxidized. This novel in vivo finding helps explain how probucol inhibits restenosis after coronary angioplasty and highlights potential new targets for therapeutic intervention.
Assessment of Negative Gingival Recession: A Critical Component of Periodontal Diagnosis (2022)

Accurate measurement of negative gingival recession (GR) is essential to accurately determine the clinical attachment loss, which leads to an accurate diagnosis and optimal therapy of periodontal disease. However, the accuracy of measuring the negative GR has been shown to be low and highly variable between examiners. The position of the gingiva margin in relation to the cemento-enamel junction (CEJ) varies among different stages of passive eruption. The amount of negative GR is about 2 mm on average at the mid-facial sites and ranges from 2 to 3.5 mm at interproximal sites in periodontally healthy patients. Some other clinical conditions may change the gingival dimension coronal to the CEJ, such as altered passive eruption and gingival enlargement. In addition to the traditional approach using a periodontal probe to assess the negative GR, nowadays dental ultrasound imaging may be able to assist in accurately measuring the amount of negative GR. This narrative review will discuss the existing evidence of the dimension of dentogingival tissue and the clinical assessment of negative GR using different clinical tools.
A Novel Aerosol Method for the Production of Hydrogel Particles (2011)

A novel method of generating hydrogel particles for various applications including drug delivery purposes was developed. This method is based on the production of hydrogel particles from sprayed polymeric nano/microdroplets obtained by a nebulization process that is immediately followed by gelation in a crosslinking fluid. In this study, particle synthesis parameters such as type of nebulizer, type of crosslinker, air pressure, and polymer concentration were investigated for their impact on the mean particle size, swelling behavior, and morphology of the developed particles. Spherical alginate-based hydrogel particles with a mean particle size in the range from 842 to 886 nm were obtained. Using statistical analysis of the factorial design of experiment it was found that the main factors influencing the size and swelling values of the particles are the alginate concentration and the air pressure. Thus, it was demonstrated that the method described in the current study is promising for the generation of hydrogel particles and it constitutes a relatively simple and low-cost system.
Structural basis for antigenic peptide precursor processing by the endoplasmic reticulum aminopeptidase ERAP1 (100.57) (2011)

Abstract ERAP1 trims antigen precursors to fit into MHC class I proteins. To perform this function, ERAP1 has unique substrate preferences, trimming long peptides while sparing shorter ones. To identify the structural basis for ERAP1’s unusual properties, we determined the X ray crystal structure of human ERAP1 bound to bestatin. The structure reveals an open conformation with a large interior compartment. An extended groove originating from the enzyme’s catalytic center can accommodate long peptides and has features that explain ERAP1’s broad specificity for antigenic peptide precursors. Structural and biochemical analysis suggest a mechanism for ERAP1’s length-dependent trimming activity, whereby binding of long but not short substrates induces a conformational change with reorientation of a key catalytic residue towards the active site. ERAP1’s unique structural elements suggest how a generic aminopeptidase structure has been adapted for the specialized function of trimming antigenic precursors.
Pressure effects on relative retention in capillary gas‐liquid chromatography (1996)

The dependence of retention values (capacity factor, relative retention, Kovats' retention index) of some organic compounds (different chemical classes) on average column pressure has been experimentally studied for three carrier gases (hydrogen, nitrogen, and carbon dioxide). Linear equations for the relative retention dependence on average pressure were theoretically deduced. The equations obtained conformed well to the experiment. Even at low pressures (2–5 atm), carrier gas pressure influence on relative retention values is usually greater than experimental error. © 1996 John Wiley & Sons, Inc.
Fifteen Years Of Laser Recording-Where We've Been And Where We're Going (1981)

One of the earliest practical applications of lasers was in the field of recording. The laser provides a source of radiation that is convenient to modu-late and sufficiently intense to affect permanent changes in materials (recording). Both signal and image recording systems have been built using silver halide film as the storage media. Now under development are optical disc techniques that ablate special coatings on the disc surface. The laser work has been funded in general because the requirements at hand could not be met by more conventional technology. The engineering of these systems has resulted in working with the ultimate in mechanical precision, the highest optical quality available, and at electrical bandwidths that push the state of the art. This paper describes the early work in laser signal recording, the significant accomplishments in image recording, and the current efforts in optical disc recording.
Mildly Increased Mechanical Nociceptive Sensitivity in REV-ERBα Knock-out Mice (2016)

Nociception is one of the most complex senses that is affected not only by external stimulation but also internal conditions. Previous studies have suggested that circadian rhythm is important in modulating nociception. REV-ERBα knock-out (KO) mice have disrupted circadian rhythm and altered mood-related phenotypes. In this study, we examined the role of REV-ERBα in inflammatory nociception. We found that the nociceptive sensitivity of KO mice was partially enhanced in mechanical nociception. However, this partial alteration was independent of the circadian rhythm. Taken together, deletion of REV-ERBα induced a mild change in mechanical nociceptive sensitivity but this alteration was not dependent on the circadian rhythm.
Selective Formation of Substituted Pyridines from Two Different Alkynes and a Nitrile:  Novel Coupling Reaction of Azazirconacyclopentadienes with Alkynes (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSelective Formation of Substituted Pyridines from Two Different Alkynes and a Nitrile: Novel Coupling Reaction of Azazirconacyclopentadienes with AlkynesTamotsu Takahashi, Fu-Yu Tsai, and Martin KotoraView Author Information Catalysis Research Center and Graduate School of Pharmaceutical Sciences, Hokkaido University CREST, Science and Technology Corporation (JST) Sapporo 060-0811, Japan Cite this: J. Am. Chem. Soc. 2000, 122, 20, 4994–4995Publication Date (Web):May 5, 2000Publication History Received8 February 2000Published online5 May 2000Published inissue 1 May 2000https://pubs.acs.org/doi/10.1021/ja000474yhttps://doi.org/10.1021/ja000474yrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1710Altmetric-Citations104LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Coupling reactions,Hydrocarbons,Nitrogen compounds,Pyridines,Selectivity Get e-Alerts
论八十年代的现实经济与经济理论——答意大利《政治和经济》杂志编辑问 (1984)

问:近10年中,不论是现实经济形势或是经济理论都发生了重大变化。请问,战后的新凯恩斯理论以何种形式存在,七十年代新的宏观经济思想取得哪些成果和还存在哪些主要问题? 答:在七十年代,宏观经济不论是在理论上还是在实践方面都发生了严重危机。首先是五十年代和六十年代出现的对凯恩斯政策盲目赞同和崇信的思潮陷入了危机。原因归纳起来有两点,一是外部经济因素(石油危机),另一个是占主导地位的经济模式内部