gebhart/patrepeval huggingface dataset:
The CXXC motif: crystal structure of an active-site variant of Escherichia coli thioredoxin (1999)

The 2.2 A crystalline structure of an oxidized active-site variant of Escherichia coli thioredoxin (Trx) has been solved. Trx is a 12 kDa enzyme which catalyzes the oxidation of dithiols and the reduction and isomerization of disulfides in other proteins. Its active site contains the common structural motif CXXC. Protein-disulfide isomerase (PDI), a 57 kDa homolog of Trx, contains four Trx-like domains. The three-dimensional structure of PDI is unknown. PDI-deficient Saccharomyces cerevisiae are inviable. An active-site variant of Trx which complements PDI-deficient yeast has the active-site sequence Cys32-Val33-Trp34-Cys35 (CVWC). The reduction potential of oxidized CVWC Trx (E degrees ' = -0.230 V) is altered significantly from that of the wild-type enzyme (E degrees ' = -0.270 V). However, the structure of the oxidized CVWC enzyme is almost identical to that of wild-type Trx. The addition of valine and tryptophan in the active site is likely to increase the reduction potential, largely by decreasing the pK(a) of the Cys32 thiol in the reduced enzyme. Unlike in wild-type Trx, significant protein-protein contacts occur in the crystal. Protein molecules related by a crystallographic twofold axis form a dimer in the crystal. The dimer forms as an extension of the twisted mixed beta-sheet which composes the backbone of each Trx structure.
Noncovalent interactions between aromatic compounds and dissolved humic acid examined by nuclear magnetic resonance spectroscopy (2004)

We examined the molecular-level interactions of aromatic compounds with a humic acid that was extensively characterized with one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. Interactions of target compounds were evaluated by monitoring their NMR relaxation properties. Solid-state NMR revealed that the humic sample contains paraffinic carbon (31%), substituted aliphatic carbon (30%), and aromatic carbon (28%). The liquid-state experiments further identified amino acids, a range of carbohydrates, methoxylated aromatics (likely derived from lignin), and a series of aliphatic chains. The 13C spin-lattice relaxation time (T1) of site-specifically labeled naphthalene, 1-naphthol, and quinoline was found to decrease with increasing additions of humic acid. The 1H T1 values also were measured and demonstrated that the association with the humic acid was not specific because all the protons acquired the T1 value of the humic acid at the same rate. The lack of a chemical shift change and an increase in signal line broadening indicates that the interaction between these compounds and humic acid is noncovalent. These interactions were detected at low humic concentrations (5 mg C/L) and suggest that low concentrations of humic material, which are prevalent in both aquatic and terrestrial systems, will significantly affect the fate and transport of contaminants in the environment.
Clinical Use of 24-Hour Gastric pH Monitoring vs O-diisopropyl Iminodiacetic Acid (DISIDA) Scanning in the Diagnosis of Pathologic Duodenogastric Reflux (1990)

The efficacy of o-diisopropyl iminodiacetic acid (DISIDA) scanning was compared with that of computerized analysis of 24-hour gastric pH monitoring to diagnose excessive duodenogastric reflux in 22 normal volunteers and 106 consecutive patients with foregut symptoms. DISIDA scanning had a false-positive rate of 18% in the normal volunteers. Gastric pH monitoring showed an increasing prevalence of duodenogastric reflux in patients with increasing clinical evidence of this condition, which was not seen with DISIDA scanning. Both DISIDA scanning and gastric pH monitoring identified duodenogastric reflux in most patients who had had previous pyloroplasty or antrectomy. Only gastric pH monitoring, however, showed a significantly increased prevalence of duodenogastric reflux in symptomatic patients after previous cholecystectomy compared with those who had not undergone previous surgery. These data suggest that 24-hour gastric pH monitoring is superior to DISIDA scanning in identifying duodenogastric reflux as a cause of foregut symptoms.
Novel Seals and Specialty Component Attachment Mechanisms for Respiratory Protection System (RESPO 21) (1992)

Abstract : This program was conducted to establish the design characteristics of attachment mechanisms for use in the mounting of mask seals and specialty components into the Respiratory Protection System 21 (RESPO 21). These design characteristics include but are not limited to keeping the seal/attachment mechanism low in profile, allowing easy seal or component replacement, and incorporating multiple layers of material within the seal and attachment mechanism. Alternative seal concepts were investigated for the interface between the mask ad the mask wearer. These concepts include pneumatic bladders, encapsulated gels, laminated foams, and combinations of the above. Prototype seals of the encapsulated gel-type and the pneumatic type were fabricated to demonstrate feasibility and assist with the development of attachment mechanisms. Approaches for attaching a seal to a mask, both hard-shell and soft- shell, were identified. None were fabricated due to time limitations and availability of appropriate seals and masks.
Transplantation of Embryonic Stem Cell-Derived Endodermal Cells into Mice with Induced Lethal Liver Damage (2007)

ESCs are a potential cell source for cell therapy. However, there is no evidence that cell transplantation using ESC-derived hepatocytes is therapeutically effective. The main objective of this study was to assess the therapeutic efficacy of the transplantation of ESC-derived endodermal cells into a liver injury model. The beta-galactosidase-labeled mouse ESCs were differentiated into alpha-fetoprotein (AFP)-producing endodermal cells. AFP-producing cells or ESCs were transplanted into transgenic mice that expressed diphtheria toxin (DT) receptors under the control of an albumin enhancer/promoter. Selective damage was induced in the recipient hepatocytes by the administration of DT. Although the transplanted AFP-producing cells had repopulated only 3.4% of the total liver mass 7 days after cell transplantation, they replaced 32.8% of the liver by day 35. However, these engrafted cells decreased (18.3% at day 40 and 7.9% at day 50) after the cessation of DT administration, and few donor cells were observed by days 60-90. The survival rate of the AFP-producing cell-transplanted group (66.7%) was significantly higher in comparison with that of the sham-operated group (17.6%). No tumors were detected by day 50 in the AFP-producing cell-transplanted group; however, splenic teratomas did form 60 days or more after transplantation. ESC transplantation had no effect on survival rates; furthermore, there was a high frequency of tumors in the ESC-transplanted group 35 days after transplantation. In conclusion, this study demonstrates, for the first time, that ESC-derived endodermal cells improve the survival rates after transplantation into mice with induced hepatocellular injury. Disclosure of potential conflicts of interest is found at the end of this article.
Incidence of placental abruption in relation to cigarette smoking and hypertensive disorders during pregnancy: a meta-analysis of observational studies (1999)

Our meta-analyses showed an increased risk for placental abruption in relation to both cigarette smoking and hypertensive disorders during pregnancy. Because cigarette smoking is a modifiable risk factor, and hypertensive disorders are potentially treatable if diagnosed early in pregnancy, patient education, smoking cessation programs, and early prenatal care may be important factors in the prevention of placental abruption.
Relationship between obesity and the increased risk of major complications in non‐insulin‐dependent diabetes mellitus (1998)

Obesity and non-insulin-dependent diabetes mellitus (NIDDM) are closely linked. They frequently occur together in patients, and body mass index (BMI) is the strongest risk factor for the development of NIDDM. Both obesity and NIDDM are also major causes of morbidity and mortality from atherogenic macrovascular disease, and they are independent risk factors for coronary heart disease. The risk of developing NIDDM and cardiovascular disease is affected by the regional distribution of body fat. Visceral obesity is associated with a higher degree of risk than peripheral obesity. The metabolic and circulatory changes associated with visceral obesity lead to the development of insulin resistance and increased lipoprotein synthesis. For example, the change in the population profile of lipoproteins in the blood, and alterations in the levels of oxidative stress lead to an increased cardiovascular and macrovascular risk. The changes in lipid metabolism also affect haemorrheological function. They have been linked to decreased fibrinolysis (a serious cardiovascular risk factor) through elevated levels of plasminogen activator inhibitor factor, high blood viscosity, and increased erythrocyte aggregability. Increased BMI also appears to be associated with endothelial dysfunction, which is a major factor in atheroma plaque formation and development of thrombosis. Visceral obesity therefore adds a significant burden to the already increased cardiovascular risk inherent in NIDDM. However, even moderate weight loss may successfully reverse the majority of changes seen with visceral obesity.
Novel selenium-oxygen and silane-nitrogen bond metatheses. Mechanistic probes, spectroscopic studies, and structural evidence for reactive oligomeric selenoazyl intermediates (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel selenium-oxygen and silane-nitrogen bond metatheses. Mechanistic probes, spectroscopic studies, and structural evidence for reactive oligomeric selenoazyl intermediatesDerek H. R. Barton and Shyamal I. ParekhCite this: J. Am. Chem. Soc. 1993, 115, 3, 948–955Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://pubs.acs.org/doi/10.1021/ja00056a019https://doi.org/10.1021/ja00056a019research-articleACS PublicationsRequest reuse permissionsArticle Views378Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Coherent combining of high brightness tapered amplifiers for efficient non-linear conversion (2019)

We report on a coherent beam combination of three high-brightness tapered amplifiers, which are seeded by a single-frequency laser at λ = 976 nm in a simple architecture with efficiently cooled emitters. The maximal combined power of 12.9 W is achieved at a combining efficiency of > 65%, which is limited by the amplifiers' intrinsic beam quality. The coherent combination cleans up the spatial profile, as the central lobe's power content increases by up to 86%. This high-brightness infrared beam is converted into the visible by second harmonic generation. This results in a high non-linear conversion efficiency of 4.5%/W and a maximum power over 2 W at 488 nm, which is limited by thermal effects in the periodically poled lithium niobate (PPLN).
Rapid Genetic Engineering of Human Cytomegalovirus by Using a Lambda Phage Linear Recombination System: Demonstration that pp28 (UL99) Is Essential for Production of Infectious Virus (2003)

A highly efficient lambda phage recombination system previously utilized for studies of bacterial artificial chromosome (BAC)-maintained mouse chromosomal DNA was adapted for the study of the role of human cytomegalovirus (HCMV)-encoded pp28 (UL99) in virus replication. Incorporating a two-step mutagenesis strategy with blue/white selection in Escherichia coli containing a HCMV AD169 BAC, we have shown that we can rapidly introduce point mutations into the HCMV BAC using linear PCR fragments. All manipulations were carried out in bacteria, which greatly accelerated the introduction and analysis of mutations in the viral genome. Our results indicated that HCMV pp28 was essential for the production of infectious virus and that introduction of a single base change that resulted in loss of the myristylation site on pp28 was also associated with the lack of production of infectious virus. Although the block in the viral morphogenesis cannot be determined from these studies, the latter finding suggested that authentic intracellular localization of pp28, not only the expression of the protein, is required for virus assembly.
P1‐018: HIPPOCAMPAL GREY MATTER ATROPHY IS ASSOCIATED WITH THE FREE AND CUED SELECTIVE REMINDING TEST (2014)

One morphological hallmark of Alzheimer's disease (AD) pathology is early atrophy of the hippocampal formation. The Free and Cued Selective Reminding Test (FCSRT) and The Consortium to Establish a Registry for Alzheimer's Disease neuropsychological battery (CERAD-NB) are widely used cognitive tests for the assessment of episodic memory in patients with AD and subjects at-risk of AD. It has been postulated that the FCSRT is more specific for memory impairment of the hippocampal type than other memory tests. One previous study showed a greater association of the FCSRT with cerebrospinal fluid (CSF) AD-type biomarker profiles compared with the CERAD memory tests. This study aims to determine the association of the FCSRT and CERAD memory tests with hippocampal atrophy in a memory clinic patient sample. The CERAD-NB and FCSRT were performed in 72 subjects [46 males; age= 71 ±8 (years)]. The sample consists of patients with AD (n=14), with mild cognitive impairment (MCI) (n= 24), with subjective cognitive decline (SCD) (n= 26) and of controls (n=8). All participants underwent 3-D T1-weighted magnetic resonance imaging (MRI) using 3-T scanners. Data processing, including segmentation, normalization and smoothing (8mm) were performed with SPM8. Voxel-wise regression analyses were undertaken, with grey matter (GM) volume as dependent variable and each memory score as independent variable (FCSRT free recall, FCSRT total recall (free+cued), CERAD immediate recall, and CERAD delayed recall), either in the entire sample or in the combined MCI/SCD subsample. Age, sex, total intracranial volume and scanner type were added as nuisance covariates. We restricted our analysis in the anatomical region of interest (bilateral hippocampus). Table 1 lists the results of the associations between each memory test score and bilateral hippocampal GM volume. The association between FCSRT scores with GM volume is greater than that of the CERAD scores with GM volume in the entire sample and also in the MCI/SCD subsample. Our results suggest that the FCSRT-scores predict hippocampal atrophy in AD and AD at-risk conditions better than the CERAD-scores. This supports the FCSRT as an instrument with greater sensitivity for hippocampal pathology than standard word list learning tests.
Comparison of oral bioavailability of two dosage-forms of progesterone in women. (1991)

For poorly water soluble drugs, the dissolution process in biological fluids the rate limiting step in absorption. However, the utilization of some galenic processes such as solid dispersions (SD) leads to an improvement in quality and intensity of the drug gastro-intestinal absorption. In a previous work, the in vitro studies of the dissolution curves of both the pure micronized progesterone (MP) and the progesterone-PEG 6000 SD revealed marked increases in the progesterone dissolution rates for all the SD investigated compared to the pure MP. The aim of this work was to investigate the in vitro results after oral administration of the two pharmaceutical forms to menopaused volunteer women.
A New Decision Rule for Model Structure Identification of A Class of Nonlinear Dynamic Systems (1989)

This paper is concerned with the problem of deriving a statistical decision to rule for model structure identification of a class of nonlinear dynamic systems. The concept of a basis to describe the model structure is introduced and the model structure space corresponding algebra structure are defined. Then, based on the Kullback-Leibler mean information, a new model structure decision rule is developed by maximising the average log-likelihood function. Some analytical and simulated comparisons of this decision rule with Akaike's FPE and AIC, F-test and Bayes aposteriori decision rule are given.
A General PWM Strategy for Four-Switch Three-Phase Inverters (2006)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">A general pulsewidth modulation (PWM) method for control of four-switch three-phase inverters is presented. The proposed vector PWM offers a simple method to select three or four vectors that effectively synthesize the desired output voltage, even in presence of voltage oscillations across the two dc-link capacitors. The method utilizes the so called space vector modulation, and includes its scalar version. Different vector combinations are compared. The effect of Wye and delta motor winding connections over the pulse width modulator is also considered. The common mode voltage generated by the four-switch three-phase converter is evaluated and compared to that provided by the standard six-switch three-phase inverter. Simulation and experimental results are presented to demonstrate the feasibility of the proposed approach.</para>
Smart Virological Modelling of YouTube Videos (2023)

It takes few hours for a new topic to be uploaded in social media and it spread like a forest fire. So it is important to study the flow of the content in social media. YouTube is one of the most growing social media platforms so the aim lies in the fact that which content uploaded in YouTube is likely to be viewed and which is likely to flow. So it would be really a task of interest that if we are able to figure out the common underlying dimensions from a whole bunch of dimensions on which the viewing factors depend we could know how to change values in order to get better view count. The social media platforms lack or somewhere deviate to provide quality features and accessibility like them. This paper brings in the concept of a complete integrated system that works on YouTube and lets the user to get a complete assistance on his/her content.
From Lignin to Three-Dimensional Interconnected Hierarchically Porous Carbon with High Surface Area for Fast and Superhigh-Efficiency Adsorption of Sulfamethazine (2017)

A novel three-dimensional lignin-based interconnected hierarchical porous carbon (3DLHPC) with very high specific surface areas (2784 m2 g–1) and large pore volumes (1.382 cm3 g–1) was prepared using sodium lignin sulfonate as carbon precursor, via confinement carbonization, etching silica-template, and in situ alkali activation, for fast and super highly efficient removal of sulfamethazine (SMZ) antibiotics from water. By batch adsorption experiments test, 3DLHPC showed a strong adsorption affinity for SMZ with the maximum monolayer adsorption capacity of 869.6 mg g–1 at 308 K. Owing to this well-defined 3D interconnected hierarchical porous structure, the adsorption equilibrium could be reached within 30 min at 298 K. The adsorption mechanism might be involved in van der Waals force, π–π EDA interaction, electronic interaction, and hydrophobic interaction, as well as hydrogen bonding interaction. Meanwhile, it was demonstrated that 3DLHPC exhibited excellent regeneration ability, showing the potential possibility for antibiotic wastewater treatments.
Room Temperature Creep and its Effect on Fatigue Crack Growth in a X70 Steel with Various Microstructures (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Exploiting D2D Communications at the Network Edge for Mission-Critical IoT Applications (2017)

The advent of the Internet of Things (IoT) is boosting a wide range of new multimedia applications driven by an ecosystems of smart and highly heterogeneous devices. This introduces new challenges for industries and network operators in the design of next-to-come fifth generation (5G) wireless systems, where stringent performance requirements in terms of high data rate, improved reliability, and ultra-low latency are to be met. A viable way of development is the integration of local clouds at the edge of the network as part of the recent Edge Computing paradigm. However, poor channel conditions experienced by the devices towards the serving edge node may impede the effectiveness of managing mission-critical IoT applications. In this context, Device-to-Device (D2D) communications represent a key enabling technology, which offers decisive benefits for future mobile 5G scenarios. As proposed in this paper, edgebased IoT applications may rely on D2D transmissions between the IoT devices also in the presence of mobility. In particular, a forwarding scheme is proposed showing that whenever collaborating IoT devices fall under the coverage of neighboring cellular edge nodes, D2D communications can guarantee a significant reduction in delay and traffic load across the network. The proposed solution is validated through simulations that indicate significant improvements in terms of latency, percentage of served tasks, energy efficiency, and traffic load w.r.t. the case where all communications are forwarded over the edge nodes.
40‐2: Invited Paper: Polymer‐Diluted Small Molecule Organic Semiconductors with Extreme Thermal Stability (2019)

We show that diluting small molecule hole transport materials through co‐evaporation with the amorphous fluoropolymer Teflon AF dramatically improves their thermal stability and lowers their refractive index without sacrificing electrical performance.