611 MICRORNA AS NOVEL BLOOD-BASED BIOMARKERS IN CLEAR CELL RENAL CELL CARCINOMA (2013)

You have accessJournal of UrologyKidney Cancer: Basic Research (IV)1 Apr 2013611 MICRORNA AS NOVEL BLOOD-BASED BIOMARKERS IN CLEAR CELL RENAL CELL CARCINOMA A Ari Hakimi, Anders Jacobsen, Nina Mikkilineni, Brandon Fiegoli, Sara Blass, Yevgeniy Grigoryev, Agnes Viale, Nicholas Socci, Martin H Voss, Robert Motzer, Victor E Reuter, Jonathan Coleman, Paul Russo, and James J Hsieh A Ari HakimiA Ari Hakimi New York, NY More articles by this author , Anders JacobsenAnders Jacobsen New York, NY More articles by this author , Nina MikkilineniNina Mikkilineni New York, NY More articles by this author , Brandon FiegoliBrandon Fiegoli New York, NY More articles by this author , Sara BlassSara Blass New York, NY More articles by this author , Yevgeniy GrigoryevYevgeniy Grigoryev New York, NY More articles by this author , Agnes VialeAgnes Viale New York, NY More articles by this author , Nicholas SocciNicholas Socci New York, NY More articles by this author , Martin H VossMartin H Voss New York, NY More articles by this author , Robert MotzerRobert Motzer New York, NY More articles by this author , Victor E ReuterVictor E Reuter New York, NY More articles by this author , Jonathan ColemanJonathan Coleman New York, NY More articles by this author , Paul RussoPaul Russo New York, NY More articles by this author , and James J HsiehJames J Hsieh New York, NY More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.162AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES MicroRNAs (miRNA) are short, non-coding RNAs involved in post-transcriptional gene regulation. Several reports have assessed their role as blood based biomarkers given their tissue and cancer-specific expression. Using an integrative approach we sequenced the miRNA transcriptome of the plasma of several clear cell renal cell carcinoma (ccRCC) patients both before and after surgery as well as several controls. METHODS We performed next generation miRNA sequencing (miRNAseq) on eight pairs (pre and postoperative plasma samples) and four non-cancer controls to identify potential biomarker candidates. We further integrated our data with the miRNAseq tumor data from the Cancer Genome Atlas (TCGA) study to determine whether plasma miRNA levels are representative of tumor miRNA expression in ccRCC. RESULTS Overall, 930 unique miRNAs were detected, including 272 at greater than 10 read counts. There was a global shift of miRNA expression toward the non-cancer controls in the postoperative samples compared to preoperative. We further identified several stably expressed miRNAs across all samples and controls including miR-16, miR-191, and miR-103. We also identified several potential biomarker candidates by looking at differential expression both in terms of preoperative and postoperative status, as well as tumor vs control including miR-378 and miR-660. Intriguingly, the plasma miRNA expression patterns showed no relationship to the tumor expression patterns using the TCGA samples. CONCLUSIONS Plasma miRNA expression patterns are consistently altered in ccRCC and, following surgery, globally revert to the non-cancerous levels of the controls. Several biomarker candidates have been identified and a panel is undergoing validation in a larger cohort. Plasma miRNA levels do not appear to reflect tumor levels in ccRCC. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e250 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information A Ari Hakimi New York, NY More articles by this author Anders Jacobsen New York, NY More articles by this author Nina Mikkilineni New York, NY More articles by this author Brandon Fiegoli New York, NY More articles by this author Sara Blass New York, NY More articles by this author Yevgeniy Grigoryev New York, NY More articles by this author Agnes Viale New York, NY More articles by this author Nicholas Socci New York, NY More articles by this author Martin H Voss New York, NY More articles by this author Robert Motzer New York, NY More articles by this author Victor E Reuter New York, NY More articles by this author Jonathan Coleman New York, NY More articles by this author Paul Russo New York, NY More articles by this author James J Hsieh New York, NY More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...