gebhart/patrepeval huggingface dataset:
Test Methodologies for Power Cycling Experiment (2009)

When compared to temperature distributions in an actual application, thermal cycling is not a complete representation of the thermal gradients found in functional electronics under power-on condition. This discrepancy is particularly severe in power electronics and it distorts the thermo-mechanical stresses experienced at the joints and interfaces of power devices. Accelerated stress tests for power electronics are therefore better conducted with accelerated power cycling experiments rather than with accelerated thermal cycling, because the power cycles simulate more closely accelerated versions of an application cycle where the junction temperature of the die rises and falls as the power is turned off and on. However, developing a power cycling test setup can be comparatively more challenging than temperature cycling test setup, because of the complex triggering circuitry and logic needed for rapid power cycling, power circuitry needed to supply the large wattage safely to the devices under test, thermal cooling system to remove the high amount of heat generated, and software/hardware to control the test setup to maintain the right operational parameters. In this study, a test setup has been developed to power cycle IGBT and bipolar semiconductor devices for accelerated durability tests. The test setup is described and the role of each hardware and software component in the test setup is elaborated. Sample test results are presented, to illustrate the capabilities of the test setup. This work adds to the state of the art of power cycling experiments and improves our understanding of ways to develop stable power cycling test setups for various kinds of applications.
Vascular endothelial growth factor (VEGF) in breast cancer: comparison of plasma, serum, and tissue VEGF and microvessel density and effects of tamoxifen. (2000)

The assessment of angiogenesis in breast cancer is of importance as a key indicator of survival and response to therapy. Circulating vascular endothelial growth factor (VEGF) measurements may provide a less subjective analysis than microvessel density (MVD) or immunohistochemical analysis of VEGF expression; however, most studies have used serum, which is now known to largely reflect platelet-derived VEGF concentrations. This study examined for the first time both plasma (VEGFp) and serum (VEGFs) VEGF concentrations in 201 blood samples from pre- and postmenopausal healthy controls and from patients with benign breast disease, localized breast cancer, breast cancer in remission, or metastatic breast cancer and related these to other clinicopathological markers. VEGFp but not VEGFs concentrations of patients with localized disease were significantly elevated compared with normal controls (P = 0.016). Patients with metastatic disease had higher VEGFp and VEGFs levels than normal controls (P < 0.001, P = 0.044 respectively), and higher VEGFp, but not VEGFs, than patients with benign disease (P = 0.009) and patients with localized disease (P = 0.004). However, the highest VEGFp and VEGFs concentrations were seen in patients in remission compared with normal controls (P < 0.001 and P = 0.008, respectively). VEGFp concentrations in patients in remission were also higher than in patients with benign disease (P = 0.01) or patients with localized disease (P = 0.005). Tamoxifen treatment was significantly associated with higher circulating and platelet-derived VEGF levels. Circulating VEGF did not correlate with any clinicopathological factor, including MVD or VEGF expression. VEGF expression was significantly correlated with estrogen receptor status and inversely correlated with tumor grade. MVD correlated with tumor size. Tamoxifen-induced increases in VEGF may be important in clinical prognosis or associated pathologies.
Investigating Confidence Histograms and Classification in FSV: Part I. Fuzzy FSV (2013)

One important aspect of the feature selective validation (FSV) method is that it classifies comparison data into a number of natural-language categories. This allows comparison data generated by FSV to be compared with equivalent “visual” comparisons obtained using the visual rating scale. Previous research has shown a close relationship between visual assessment and FSV generated data using the resulting confidence histograms. In all cases, the category membership functions are “crisp”: that is data on the FSV value axis fall distinctly into one category. An important open question in FSV-based research, and for validation techniques generally, is whether allowed variability in these crisp category membership functions could further improve agreement with the visual assessment. A similar and related question is how robust is FSV to variation in the categorization algorithm. This paper and its associated “part II” present research aimed at developing a better understanding of the categorization of both visual and FSV data using nonsquare or variable boundary category membership functions. This first paper investigates the level of improvement to be expected by applying fuzzy logic to location of the category boundaries. The result is limited improvement to FSV, showing that FSV categorization is actually robust to variations in category boundaries.
Corneal power measurement with Fourier-domain optical coherence tomography (2010)

Corneal power measured by Fourier-domain OCT achieved good repeatability in all 3 groups. The repeatability was better than slower time-domain OCT systems. Because Fourier-domain OCT directly measures both anterior and posterior corneal surfaces, it may produce more consistent results than standard keratometry in post-LASIK and keratoconic eyes in which the anterior-posterior corneal curvature ratios are altered by surgery or disease.
Protein area occupancy at the center of the red blood cell membrane (2008)

In the Fluid Mosaic Model for biological membrane structure, proposed by Singer and Nicolson in 1972, the lipid bilayer is represented as a neutral two-dimensional solvent in which the proteins of the membrane are dispersed and distributed randomly. The model portrays the membrane as dominated by a membrane lipid bilayer, directly exposed to the aqueous environment, and only occasionally interrupted by transmembrane proteins. This view is reproduced in virtually every textbook in biochemistry and cell biology, yet some critical features have yet to be closely examined, including the key parameter of the relative occupancy of protein and lipid at the center of a natural membrane. Here we show that the area occupied by protein and lipid at the center of the human red blood cell (RBC) plasma membrane is at least approximately 23% protein and less than approximately 77% lipid. This measurement is in close agreement with previous estimates for the RBC plasma membrane and the recently published measurements for the synaptic vesicle. Given that transmembrane proteins are surrounded by phospholipids that are perturbed by their presence, the occupancy by protein of more than approximately 20% of the RBC plasma membrane and the synaptic vesicle plasma membrane implies that natural membrane bilayers may be more rigid and less fluid than has been thought for the past several decades, and that studies of pure lipid bilayers do not fully reveal the properties of lipids in membranes. Thus, it appears to be the case that membranes may be more mosaic than fluid, with little unperturbed phospholipid bilayer.
Connectionist Optimisation of Tied Mixture Hidden Markov Models (1991)

Issues relating to the estimation of hidden Markov model (HMM) local probabilities are discussed. In particular we note the isomorphism of radial basis functions (RBF) networks to tied mixture density modelling; additionally we highlight the differences between these methods arising from the different training criteria employed. We present a method in which connectionist training can be modified to resolve these differences and discuss some preliminary experiments. Finally, we discuss some outstanding problems with discriminative training.
Online monitoring of crevice corrosion with electrochemical noise (2004)

Abstract The time‐related cumulated noise charges extracted from the noise signals between two “identical” crevice electrodes yield a direct quantitative easy‐to‐interpret information on time‐related corrosion intensities in the crevice. A new software‐assisted method of noise data evaluation allows a “counting of coulombs” exchanged between the system electrodes, the results of which directly correlate with the metal dissolution intensity in the crevice. The versatility of this “CoulCount” method is demonstrated in a failure analysis on the cause of crevice corrosion at spigots of automotive cooling water pumps.
Discovery of Novel Allopurinol Derivatives with Anticancer Activity and Attenuated Xanthine Oxidase Inhibition (2016)

A series of pyrazolo[3,4-d]pyrimidine derivatives related to allopurinol has been synthesized and evaluated for its cytotoxicity against a panel of three cancer cell lines as well as its xanthine oxidase (XOD) inhibitory activities. Among them, compound 4 showed potent cytotoxicity with IC50 values of 25.5 and 35.2 μM against human hepatoma carcinoma cell lines, BEL-7402 and SMMC-7221, respectively. The anticancer activity of 4 was comparable to that of Tanespimycin (17-N-allylamino-17-demethoxy geldanamycin, 17-AAG) that inhibited the growth of BEL-7402 and SMMC-7221 cells at IC50 values of 12.4 and 9.85 μM, respectively. However, unlike allopurinol, which is also a strong inhibitor of XOD, compound 4 is a much weaker XOD inhibitor, suggesting that the anticancer activities of the allopurinol derivatives may not be associated with XOD inhibition. Moreover, the cytotoxicity of 4 toward normal cells is significantly lower than that of 17-AAG, making 4 a promising lead compound for further optimization of structure-activity relationships that may lead to anticancer agents of clinical utility.
Particle Size Analysis In Pharmaceutics And Other Industries: Theory And Practice (1992)

Recent major advances in particle size analysis, particularly with regard to its application in the pharmaceutical and related industries, provides justification for this title. It is a book for technicians and senior technicians, project and development managers, and formulation More...development scientists in a wide range of industries, pharmace
We don't Need Thousand Proposals: Single Shot Actor-Action Detection in Videos (2021)

We propose SSA2D, a simple yet effective end-to-end deep network for actor-action detection in videos. The existing methods take a top-down approach based on region-proposals (RPN), where the action is estimated based on the detected proposals followed by post-processing such as non-maximal suppression. While effective in terms of performance, these methods pose limitations in scalability for dense video scenes with a high memory requirement for thousands of proposals. We propose to solve this problem from a different perspective where we don't need any proposals. SSA2D is a unified network, which performs pixel level joint actor-action detection in a single-shot, where every pixel of the detected actor is assigned an action label. SSA2D has two main advantages: 1) It is a fully convolutional network which does not require any proposals and post-processing making it memory as well as time efficient, 2) It is easily scalable to dense video scenes as its memory requirement is independent of the number of actors present in the scene. We evaluate the proposed method on the Actor-Action dataset (A2D) and Video Object Relation (VidOR) dataset, demonstrating its effectiveness in multiple actors and action detection in a video. SSA2D is 11x faster during inference with comparable (sometimes better) performance and fewer network parameters when compared with the prior works. Code available at https://github.com/aayushjr/ssa2d.
Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells (1990)

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation. An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO 2 . The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.
Thermal modelling investigation of heat paths due to iron losses in synchronous machines (2008)

The prediction and distribution of operational iron losses in synchronous machines are of great importance in order to improve the design procedure and yield more efficient generators. To achieve this, finite element analysis was utilised to analyse a Cummins Generator Technologies alternator and, using the required test data and material properties, the iron loss distribution across the machine's cross-section was established. From this, Lumped Circuit Coefficients (LCCs) were created. LCCs are used to translate the generator's thermal model to a lumped circuit thermal network.
Abstract 331: Dynamic kinome targeting reveals kinases involved in acquired resistance to tyrosine kinase inhibitors in EGFR-driven glioblastomas (2019)

Abstract Glioblastoma (GBM) is a devastating primary brain tumor with limited treatment options. Extensive molecular characterization has revealed two particularly frequent mutations: CDKN2A deletion (50-60%) and EGFR (40-50%). EGFRvIII (~35%) is a constitutively active truncation mutant with exons 2-7 deleted. EGFR is a particularly attractive therapeutic target due to frequent activating mutations, such as EGFRvIII, and ready availability of multiple targeted inhibitors. Several EGFR tyrosine kinase inhibitors (TKI) have failed clinically, due in part to acquired resistance. To mechanistically examine this type of resistance, we used genetically-engineered mouse astrocytes harboring homozygous deletions of Cdkn2a, as well as EGFRvIII (CEv3). CEv3 astrocytes were made intrinsically resistant to the EGFR TKI gefitinib or erlotinib via long-term exposure, both in vitro and in vivo. We found that long-term gefitinib or erlotinib exposure conferred variable levels of cross resistance to a panel of second- and third-generation EGFR TKI (ΔIC50 1.12-36.1-fold), relative to non-resistant parent lines. We have previously shown that dynamic kinome reprogramming may be responsible for TKI resistance in glioblastoma. Therefore, we used a chemical proteomics method, multiplexed inhibitor beads and mass spectrometry (MIB-MS), to examine changes in the expressed and functional kinome, in both the presence or absence of one of several EGFR TKI known to penetrate the blood-brain barrier. Additionally, we performed RNA sequencing (RNA-seq) to inspect transcriptomic alterations in response to these drugs. RNA-seq showed that resistant CEv3 mouse astrocytes clustered separately from their non-resistant in vitro and in vivo counterparts. Acquired resistance also induced transcriptome alterations governing cellular metabolism, including upregulation of metabolic pathways and downregulation of RNA processing genes. Importantly, the kinase transcriptome was rewired, as 67 kinases were differentially expressed across parental and resistant cell lines (Q&amp;lt;0.001). Probing the dynamic kinome response to afatinib, an EGFR TKI, using RNA-seq identified two potential kinases involved in acute, adaptive resistance to afatinib, Bmx and Ntrk3. Integrated kinome profiling using RNA-seq and MIB-MS in murine models of GBM with defined mutational profiles provides a powerful framework to define novel therapeutic targets that could significantly alter current treatment paradigms. Citation Format: Abby Shelton, Erin Smithberger, Madison Butler, Alex Flores, Ryan Bash, Steve Angus, Noah Sciaky, Harshil Dhruv, Gary L. Johnson, Michael E. Berens, Frank Furnari, C. Ryan Miller. Dynamic kinome targeting reveals kinases involved in acquired resistance to tyrosine kinase inhibitors in EGFR-driven glioblastomas [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 331.
The Role of Innate Immune Cells in Nonalcoholic Fatty Liver Disease (2021)

Nonalcoholic fatty liver disease (NAFLD) is a very common hepatic pathology featuring steatosis and is linked to obesity and related conditions, such as the metabolic syndrome. When hepatic steatosis is accompanied by inflammation, the disorder is defined as nonalcoholic steatohepatitis (NASH), which in turn can progress toward fibrosis development that can ultimately result in cirrhosis. Cells of innate immunity, such as neutrophils or macrophages, are central regulators of NASH-related inflammation. Recent studies utilizing new experimental technologies, such as single-cell RNA sequencing, have revealed substantial heterogeneity within the macrophage populations of the liver, suggesting distinct functions of liver-resident Kupffer cells and recruited monocyte-derived macrophages with regards to regulation of liver inflammation and progression of NASH pathogenesis. Herein, we discuss recent developments concerning the function of innate immune cell subsets in NAFLD and NASH.
Trajectory clustering and its applications for video surveillance (2005)

In this paper we present a trajectory clustering method suited for video surveillance and monitoring systems. The clusters are dynamic and built in real-time as the trajectory data is acquired, without the need of an off-line processing step. We show how the obtained clusters can be successfully used both to give proper feedback to the low-level tracking system and to collect valuable information for the high-level event analysis modules.
Decorin, erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 regulation of semaphorin 3A in central nervous system scar tissue (2010)

Scar tissue at sites of traumatic injury in the adult central nervous system presents a combined physical and molecular impediment to axon regeneration. Of multiple known central nervous system scar associated axon growth inhibitors, semaphorin 3A has been shown to be strongly expressed by invading leptomeningeal fibroblasts. We have previously demonstrated that infusion of the small leucine-rich proteoglycan decorin results in major suppression of several growth inhibitory chondroitin sulphate proteoglycans and growth of adult sensory axons across acute spinal cord injuries. Furthermore, decorin treatment of leptomeningeal fibroblasts significantly increases their ability to support neurite growth of co-cultured adult dorsal root ganglion neurons. In the present study we show that decorin has the ability to suppress semaphorin 3A expression within adult rat cerebral cortex scar tissue and in primary leptomeningeal fibroblasts in vitro. Infusion of decorin core protein for eight days resulted in a significant reduction of semaphorin 3A messenger RNA expression within injury sites compared with saline-treated control animals. Both in situ hybridization and immunostaining confirmed that semaphorin 3A messenger RNA expression and protein levels are significantly reduced in decorin-treated animals. Similarly, decorin treatment decreased the expression of semaphorin 3A messenger RNA in cultured rat leptomeningeal fibroblasts compared with untreated cells. Mechanistic studies revealed that decorin-mediated suppression of semaphorin 3A critically depends on erythroblastic leukaemia viral oncogene homologue B4 and signal transducer and activator of transcription 3 function. Collectively, our studies show that in addition to suppressing the levels of inhibitory chondroitin sulphate proteoglycans, decorin has the ability to suppress semaphorin 3A in the injured central nervous system. Our findings provide further evidence for the use of decorin as a potential therapy for promoting axonal growth and repair in the injured adult mammalian brain and spinal cord.
Latent class analysis of two-way contingency tables by Bayesian methods (1989)

Latent class analysis in two-way contingency tables usually suffers from unidentifiability problems. These an be overcome by using Bayesian techniques in which prior distributions are assumed on the latent parameters. Application of these techniques, however, involves some unusual computational difficulties. Bayesian techniques suitable for latent class analyses together with a remedy to the computational difficulties are discussed in this paper.