gebhart/patrepeval huggingface dataset:
Networking Applications on Unix System V Release 4 (1993)

Tapping into the powerful networking capabilities synonymous with UNIX operating systems, author Michael Padovano introduces you to the networking features of UNIX System V Release 4 - a state-of-the-art networking platform. Padovano begins by showing how existing commands work and explains how to incorporate them into new applications. He then demonstrates how to use the lower-level SVR4 networking routines to create C programs that implement more sophisticated services. He also provides actual C code and shell scripts that implement SVR4 networking applications. If you are interested in understanding how SVR4 networking utilities work and/or want to develop network applications using SVR4 routines like TLI, RPC, and Sockets, Networking Applications on UNIX System V Release 4 will be an indispensable addition to your classroom or personal reference library.
A program to develop a high-energy density primary battery with a minimum of 200 watt hours per pound of total battery weight Quarterly report, 1 Oct. - 31 Dec. 1966 (1966)

High energy density primary battery - film formation on cupric fluoride cathodes discharged in organic electrolytes
Reducing motion artifacts in Gadoxetate-enhanced Multiphasic Magnetic Resonance Imaging of the Liver by the use of U-NET network (2018)

Poster: ECR 2019 / C-0208 / Reducing motion artifacts in Gadoxetate-enhanced Multiphasic Magnetic Resonance Imaging of the Liver by the use of U-NET network by: M.-L. Kromrey 1, D. Tamada2, H. Johno2, H. Onishi2, U. Motosugi2; 1Greifswald/DE, 2Yamanashi/JP
Interactions of Concanavalin A with the Membrane of Influenza Virus Infected Cells and with Envelope Components of the Virus Particle (1972)

Treatment of host cells with Concanavalin A prevents the assembly or release of fowl plague virus from chick fibroblasts and inhibits fusion of BHK cells by the paramyxovirus SV5. Metabolic events of the host cells are not greatly impaired during the early phase of infection. There is evidence that the carbohydrate moiety of the Con A receptor of fowl plague virus is associated with the viral neuraminidase.
Facile Control over the Supramolecular Ordering of Self-assembled Peptide Scaffolds by Simultaneous Assembly with a Polysacharride (2017)

Enabling control over macromolecular ordering and the spatial distribution of structures formed via the mechanisms of molecular self-assembly is a challenge that could yield a range of new functional materials. In particular, using the self-assembly of minimalist peptides, to drive the incorporation of large complex molecules will allow a functionalization strategy for the next generation of biomaterial engineering. Here, for the first time, we show that co-assembly with increasing concentrations of a highly charged polysaccharide, fucoidan, the microscale ordering of Fmoc-FRGDF peptide fibrils and subsequent mechanical properties of the resultant hydrogel can be easily and effectively manipulated without disruption to the nanofibrillar structure of the assembly.
Involvement of the PI3K/Akt/Nrf2 Signaling Pathway in Resveratrol-Mediated Reversal of Drug Resistance in HL-60/ADR Cells (2019)

Resistance to chemotherapy drugs, such as adriamycin (ADR), is a common problem in acute myeloid leukemia (AML) patients. We hypothesized that the natural compound resveratrol (Res) may reverse AML drug resistance through the PI3K/Akt/Nrf2 pathway. We investigated the in vitro effect of Res using human promyelocytic leukemia cells (HL-60) and the ADR-resistant cell line (HL-60/ADR) and treated with either Res or ADR + Res. Cellular proliferation inhibition rate, auto-fluorescence intensity of ADR in HL-60/ADR cells and HL-60 cells, mRNA expression of <i>Nrf2</i> and the drug-resistant gene <i>MRP1</i>, and protein expression of PI3K, Akt, p-Akt, Nrf2, and MRP1 were measured. Results showed ADR + Res had a more significant inhibitory effect than ADR alone on HL-60/ADR cells. Auto-fluorescence intensity of ADR in HL-60/ADR cells treated with ADR + Res significantly increased. No difference of the auto-fluorescence intensity of ADR was observed in HL-60 cells treated with ADR and ADR + Res. mRNA expression of <i>Nrf2</i> and <i>MRP1</i> significantly decreased in HL-60/ADR cells treated with both Res and ADR + Res; protein expression of PI3K, p-Akt, Nrf2, and MRP1 significantly decreased in HL-60/ADR cells treated with PI3K inhibitor, Res and ADR + Res. In conclusion, Res reverses the drug resistance of AML HL-60/ADR cells through regulation of the PI3K/Akt/Nrf2 signaling pathway and MRP1 expression.
Strengthening the reporting of genetic risk prediction studies: the GRIPS statement (2011)

The number of known genetic markers of risk is increasing but the interpretation of their clinical effect is hampered by poor reporting of prediction studies. These guidelines from the GRIPS group aim to ensure transparent reporting of prediction studies
Production of MPS VII mouse (Gustm(hE540Amiddle dotmE536A)Sly) doubly tolerant to human and mouse beta-glucuronidase (2003)

Mucopolysaccharidosis VII (MPS VII, Sly syndrome) is an autosomal recessive lysosomal storage disease caused by beta-glucuronidase (GUS) deficiency. A naturally occurring mouse model of that disease has been very useful for studying experimental approaches to therapy. However, immune responses can complicate evaluation of the long-term benefits of enzyme replacement or gene therapy delivered to adult MPS VII mice. To make this model useful for studying the long-term effectiveness and side effects of experimental therapies delivered to adult mice, we developed a new MPS VII mouse model, which is tolerant to both human and murine GUS. To achieve this, we used homologous recombination to introduce simultaneously a human cDNA transgene expressing inactive human GUS into intron 9 of the murine Gus gene and a targeted active site mutation (E536A) into the adjacent exon 10. When the heterozygote products of germline transmission were bred to homozygosity, the homozygous mice expressed no GUS enzyme activity but expressed inactive human GUS protein highly and were tolerant to immune challenge with human enzyme. Expression of the mutant murine Gus gene was reduced to about 10% of normal levels, but the inactive murine GUS enzyme also conferred tolerance to murine GUS. This MPS VII mouse model should be useful to evaluate therapeutic responses in adult mice receiving repetitive doses of enzyme or mice receiving gene therapy as adults. Heterozygotes expressed only 9.5-26% of wild-type levels of murine GUS instead of the expected 50%, indicating a dominant-negative effect of the mutant enzyme monomers on the activity of GUS tetramers in different tissues. Corrective gene therapy in this model should provide high enough levels of expression of normal GUS monomers to overcome the dominant negative effect of mutant monomers on newly synthesized GUS tetramers in most tissues.
Using tomography images to study the mixing of wheat straw slurries (2016)

Wheat straw is a good renewable source for the production of bioethanol. However, the mixing of wheat straw slurry is a challenging task due to its complex rheology. This type of slurry behaves as a non-Newtonian fluid possessing a yield stress. In mixing operations, the presence of a yield stress creates a region of active motion (cavern) around the impeller and stagnant zones in the remainder of the vessel. In this paper, electrical resistance tomography (ERT) was employed to measure the dimensions of the cavern around the impeller as a function of the wheat straw concentration, fiber size, impeller speed, and impeller type. These data were then utilized to estimate the yield stress of wheat straw slurries. To test the accuracy of this technique, the yield stress of a xanthan gum solution calculated from the tomography method was compared to that achieved using a rheometer. This study is the first novel application of ERT to estimate the yield stress of wheat straw slurries, as opposed to directly measuring it using rheometry. Average yield stresses of 5, 7, and 10 wt% slurries were found to be 1.31 Pa, 4.2 Pa, and 14.8 Pa, respectively, for fiber size of ≤ 2 mm, and 3.4 Pa, 6.8 Pa, and 16.7 Pa, respectively, when fiber size was 8 mm.
Optimal Coordinated Motions of Multiple Agents Moving on a Plane (2003)

We address the problem of optimal coordinated motions of multiple agents moving in the same planar region. The agents' motions must satisfy a separation constraint throughout the encounter to be conflict-free. The objective is to determine the conflict-free maneuvers (motions) with the least combined energy, while taking into account the fact that agents may have different priorities. A formal classification of conflict-free maneuvers into homotopy types is introduced by using their braid representation. Various local and global optimality conditions are derived through variational analysis in the presence of the separation constraint. In the case of two agents, these optimality conditions allow us to construct the optimal maneuvers geometrically. For the general multi-agent case, a convex optimization algorithm is proposed to compute within each homotopy type a solution to the optimization problem restricted to the class of multilegged maneuvers. Since the number of types grows explosively with the number of agents, a stochastic algorithm is suggested as the "type chooser," thus leading to a randomized optimization algorithm.
Novel inflammatory breast cancer cell line, MDA-IBC-1 expresses and secretes WISP3, a putative tumor suppressor in inflammatory breast cancer. (2009)

Abstract Abstract #1051 Wnt-1-induced secreted protein 3 (WISP3) is a member of the CCN family of proteins, which also includes connective tissue growth factor (CTGF), Cy61, Nov, WISP1, and WISP2. WISP3, as well as the other CCN family members, plays important roles in cellular processes such as proliferation, migration, and survival. WISP3 was originally isolated as Lost in Inflammatory Breast Cancer (LIBC) based on its expression in only 20% of human IBC tumors. Lack of WISP3 expression has also been reported in the IBC-derived cell line SUM-149. In SUM149 cells WISP3 has been reported to function as a tumor suppressor capable of decreasing proliferation and maintaining an epithelial phenotype. Here we examine the expression of WISP3 in a novel aggressive IBC cell line with epithelial-mesenchymal transition (EMT) morphology and abundant WISP3 expression. MDA-IBC-1 cells were isolated from the pleural fluid of a patient with ER+, PR-, HER2/neu- IBC. In 2-dimensional (2D) adherent culture, MDA-IBC-1 cells exhibit fibroblast-like EMT morphology and express EMT-associated proteins such as N-cadherin, vimentin, and fibronectin. Correspondingly, E-cadherin protein expression is absent in 2D cultures. MDA-IBC-1 cells derived from 2D cultures form mammospheres when cultured in 3-dimensional (3D) progenitor promoting conditions. Interestingly, the expression of EMT molecular markers is absent in 3D progenitor promoting mammosphere culture. MDA-IBC-1 cells exhibit robust WISP3 protein expression in both 2D and 3D culture (1.8-fold higher in 3D). In addition, MDA-IBC-1 cells secrete WISP3 as indicated by the presence of WISP3 protein in MDA-IBC-1 derived conditioned media. WISP3 binding is reported to decrease insulin-like growth factor-1 (IGF-1) activation of the IGF1R signaling cascade in SUM-149 cells. IGFR1 protein is present in 2D and 3D cultured MDA-IBC-1 cells. IGF-1 protein overlay assays using conditioned media demonstrate a WISP3:IGF-1 interaction, suggesting functional folding. Serum-free MDA-IBC-1 conditioned media did not increase basal SUM149 proliferation and it inhibited IGF-1 stimulated proliferation to serum-free (basal) levels (p≤0.05). In addition, culturing SUM-149 cells in conditioned media obtained from IBC-1 cells significantly alters SUM-149 to a completely fibroblastic morphology. These data demonstrate that MDA-IBC-1 cells represent an IBC cell line with novel expression and regulation of WISP3. MDA-IBC-1 cells may better represent the cohort of IBC patients whose tumors express WISP3. This data suggest WISP3 may not function as a tumor suppressor in all IBC patients. Citation Information: Cancer Res 2009;69(2 Suppl):Abstract nr 1051.
Differential expression of sphingolipids in MRP1 overexpressing HT29 cells (2000)

We have obtained a novel multidrug resistant cell line, derived from HT29 G(+) human colon carcinoma cells, by selection with gradually increasing concentrations of the anti-mitotic, microtubule-disrupting agent colchicine. This HT29(col) cell line displayed a 25-fold increase in colchicine resistance and exhibited cross-resistance to doxorubicin, VP16, vincristine and taxol. Immunoblotting, combined with RT-PCR showed that the multidrug resistance phenotype was conferred by specific overexpression of the multidrug resistance protein 1. Confocal scanning laser microscopy revealed that multidrug resistance protein 1 specifically localized in the plasma membrane of HT29(col) cells. In a functional assay, using the fluorescent multidrug resistance protein 1 substrate 5-carboxyfluorescein, an increased efflux activity of HT29(col) cells was measured, as compared to the wild-type HT29 G(+) cells. MK571, a specific inhibitor of multidrug resistance protein 1, blocked the 5-carboxyfluorescein efflux, but only partially reversed resistance to colchicine, indicating that additional multidrug resistance mechanisms operate in HT29(col) cells. In conclusion, these results show for the first time overexpression of a functional multidrug resistance protein 1 under colchicine pressure, indicating that colchicine is not a P-glycoprotein-specific substrate. Colchicine-induced overexpression of multidrug resistance protein 1 is accompanied by a changed sphingolipid composition, i.e., enhanced levels of glucosylceramide and galactosylceramide. In addition, ceramide, a lipid messenger molecule involved in apoptosis-related signal transduction processes, was much more abundant in HT29(col) cells, which is indicative of a stress response.
Uno studio sul comportamento statico non-lineare dei ponti di grande luce (2012)

Dottorato di Ricerca in Ingegneria dei Materiali e delle Strutture, Ciclo XXV, 2009-2012
Gap-null free spectral response of asymmetric ring heads for longitudinal and perpendicular recording (1990)

The head field distribution and the signal reproducibility of asymmetric ring heads at several large inclination angles are examined. It is shown that the asymmetric ring heads with large inclination can generate sharp spike-like sensitivity functions and a good smooth spectral-response curve which has no gap-nulls up to very high k(=2 pi / lambda ) values for both perpendicular and longitudinal recording modes. Due to the sharpness in the sensitivity function distribution, the phase angles of the readout voltage vary linearly with k. The importance of symmetry and sharpness in the sensitivity distribution function is discussed in relation to the reading process. It is concluded that an asymmetric ring head with a 30 degrees inclination angle from the horizontal is very appropriate for both high-density perpendicular and longitudinal recording.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Perceived health moderates the association between smoking rate and panic vulnerability variables among daily smokers (2006)

Our aim in this investigation was to evaluate the moderating role of perceived health in the relation between smoking rate and panic variables in a community-based sample of 220 daily smokers (98 females; M(age)=23.76 years, SD=8.76). As hypothesized, the interaction between smoking and perceived health incrementally predicted anxiety sensitivity (fear of anxiety) and anxious arousal symptoms, but not depressive symptoms. Individuals who had higher smoking rates and lower perceived health reported higher levels of anxiety sensitivity and anxious arousal, but not depressive symptoms. The primary implication of these findings is that there may be segments of the cigarette smoking population who are at relatively greater risk for anxiety symptoms and fear of bodily sensations by virtue of individual differences in perceived health. The identification of such moderating effects is clinically important, because it helps to refine our understanding of complex associations between drug behavior and panic vulnerability.
Leukemia Inhibitory Factor Enhances Endometrial Stromal Cell Decidualization in Humans and Mice (2011)

Adequate differentiation or decidualization of endometrial stromal cells (ESC) is critical for successful pregnancy in humans and rodents. Here, we investigated the role of leukemia inhibitory factor (LIF) in human and murine decidualization. Ex vivo human (H) ESC decidualization was induced by estrogen (E, 10(-8) M) plus medroxyprogesterone acetate (MPA, 10(-7) M). Exogenous LIF (≥50 ng/ml) induced STAT3 phosphorylation in non-decidualized and decidualized HESC and enhanced E+MPA-induced decidualization (measured by PRL secretion, P<0.05). LIF mRNA in HESC was down-regulated by decidualization treatment (E+MPA) whereas LIF receptor (R) mRNA was up-regulated, suggesting that the decidualization stimulus 'primed' HESC for LIF action, but that factors not present in our in vitro model were required to induce LIF expression. Ex vivo first trimester decidual biopsies secreted >100 pg/mg G-CSF, IL6, IL8, and MCP1. Decidualized HESC secreted IL6, IL8, IL15 and MCP1. LIF (50 ng/ml) up-regulated IL6 and IL15 (P<0.05) secretion in decidualized HESC compared to 0.5 ng/ml LIF. In murine endometrium, LIF and LIFR immunolocalized to decidualized stromal cells on day 5 of gestation (day 0 = day of plug detection). Western blotting confirmed that LIF and the LIFR were up-regulated in intra-implantation sites compared to inter-implantation sites on Day 5 of gestation. To determine the role of LIF during in vivo murine decidualization, intra-peritoneal injections of a long-acting LIF antagonist (PEGLA; 900 or 1200 µg) were given just post-attachment, during the initiation of decidualization on day 4. PEGLA treatment reduced implantation site decidual area (P<0.05) and desmin staining immuno-intensity (P<0.05) compared to control on day 6 of gestation. This study demonstrated that LIF was an important regulator of decidualization in humans and mice and data provides insight into the processes underlying decidualization, which are important for understanding implantation and placentation.
scAAVengr: Single-cell transcriptome-based quantification of engineered AAVs in non-human primate retina (2020)

Abstract Adeno-associated virus (AAV)-mediated gene therapies are rapidly advancing to the clinic, and AAV engineering has resulted in vectors with increased ability to deliver therapeutic genes. Although the choice of vector is critical, quantitative comparison of AAVs, especially in large animals, remains challenging. Here, we developed an efficient single-cell AAV engineering pipeline (scAAVengr) to quantify efficiency of AAV-mediated gene expression across all cell types. scAAVengr allows for definitive, head-to-head comparison of vectors in the same animal. To demonstrate proof-of-concept for the scAAVengr workflow, we quantified – with cell-type resolution – the abilities of naturally occurring and newly engineered AAVs to mediate gene expression in primate retina following intravitreal injection. A top performing variant, K912, was used to deliver SaCas9 and edit the rhodopsin gene in macaque retina, resulting in editing efficiency similar to infection rates detected by the scAAVengr workflow. These results validate scAAVengr as a powerful method for development of AAV vectors.
A thermodynamic analysis of propagating subcritical cracks with cohesive zones (1993)

The results of the so-called energetic approach to fracture with particular attention to the issue of energy dissipation due to crack propagation are applied to the case of a crack with cohesive zone. The thermodynamic admissibility of subcritical crack growth (SCG) is discussed together with some hypotheses that lead to the derivation of SCG laws. A two-phase cohesive zone model for discontinuous crack growth is presented and its thermodynamics analyzed, followed by an example of its possible application.
Aziridine‐Mediated Asymmetric Synthesis of Quaternary β‐Amino Acids Using 2H‐Azirine 2‐Carboxylate Esters. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Studies on Recombination within the Mouse H‐2 Gene Complex. III. Further Serological Analyses of the H‐2t Haplotypes* (1975)

The five H-2t haplotypes are all intra-H-2 recombinants which have identical K and D regions, but differ in the central regions of the complex. Analyses of H-2, Ia and Ss-Slp types indicate that the crossover in H-2t1 took place between the K and I regions, in the H-2t2 between G and D, in H-2t3 and H-2t4 between the I-B and I-C subregions and in H-2t5 between I and S. The set of recombinants will be very valuable for studies of traits which map in the I and S regions.
Ion energy effects in AlN thin films grown on Si(111) (1996)

We report growth of epitaxial AlN thin films on Si(111) in a N2 glow discharge using a modified direct current magnetron sputter source in ultrahigh vacuum. Using such a device, we show that the plasma potential of the glow discharge can be varied over a wide range without significant changes in film growth rate and ion density. We show further that changing the plasma potential significantly and systematically affects the lattice strain and the mosaic structure of the AlN films. We show significant straining of the AlN lattice and suggest the occurrence of AlN lattice damage when N+2 ions acquire energies in excess of +50 eV.
Cellular Uptake and Transport of Zein Nanoparticles: Effects of Sodium Caseinate (2013)

Cellular evaluation of zein nanoparticles has not been studied systematically due to their poor redispersibility. Caseinate (CAS)-stabilized zein nanoparticles have been recently developed with better redispersibility in salt solutions. In this study, zein-CAS nanoparticles were prepared with different zein/CAS mass ratios. The prepared nanoparticles demonstrated good stabilities to maintain particle size (120-140 nm) in cell culture medium and HBSS buffer at 37 °C. The nanoparticles showed no cytotoxicity for Caco-2 cells for 72 h. CAS not only significantly enhanced cell uptake of zein nanoparticles in a concentration- and time-dependent manner but also remarkably improved epithelial transport through Caco-2 cell monolayer. The cell uptake of zein-CAS nanoparticles indicated an energy-dependent endocytosis process as evidenced by cell uptake under blocking conditions, that is, 4 °C, sodium azide, and colchicine. Fluorescent microscopy clearly showed the internalization of zein-CAS nanoparticles. This study may shed some light on the cellular evaluations of hydrophobic protein nanoparticles.
NORTHWESTERN UNIVERSITY'S PILOT UROLOGY RESIDENCY CURRICULUM: IMPACT OF FLEXIBLE TRAINING AND RESIDENCY ELECTIVES ON EDUCATIONAL OUTCOMES (2009)

You have accessJournal of Urology1 Apr 2009NORTHWESTERN UNIVERSITY'S PILOT UROLOGY RESIDENCY CURRICULUM: IMPACT OF FLEXIBLE TRAINING AND RESIDENCY ELECTIVES ON EDUCATIONAL OUTCOMES Stephanie J Kielb and Anthony J Schaeffer Stephanie J KielbStephanie J Kielb More articles by this author and Anthony J SchaefferAnthony J Schaeffer More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(09)60549-8AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "NORTHWESTERN UNIVERSITY'S PILOT UROLOGY RESIDENCY CURRICULUM: IMPACT OF FLEXIBLE TRAINING AND RESIDENCY ELECTIVES ON EDUCATIONAL OUTCOMES." The Journal of Urology, 181(4S), pp. 190–191 © 2009 by American Urological AssociationFiguresReferencesRelatedDetails Volume 181Issue 4SApril 2009Page: 190-191 Advertisement Copyright & Permissions© 2009 by American Urological AssociationMetricsAuthor Information Stephanie J Kielb More articles by this author Anthony J Schaeffer More articles by this author Expand All Advertisement PDF downloadLoading ...