gebhart/patrepeval huggingface dataset:
PILO-SEBACEOUS DUCT OBSTRUCTION AND ACNE (1972)

Summary.— The outermost part of the epidermis, including part of the pilo‐sebaceous apparatus, has been sampled, using a cyanoacryalnte adhesive, in patients with acne of varying severity, controls, and in acne patients treated with long term tetracycline. At the site sampled (left upper hack) patients with acne have an increased amount of inspissated material as compared with controls; this appears increased in relation to the severity of the acne. The inspissated material, which is partly keratin and partly lipid, could possibly obstruct the outflow of sebum from the duct.
Static Charge in Aircraft Fuel Tanks. (1980)

This program investigates electrostatic hazards associated with aircraft fueling. The tribo electric phenomena of reticulated polyurethane foam charging was examined using JP-4. An attempt was made to understand the basic electrostatic phenomena involved when fuel is pumped into aircraft fuel tanks containing reticulated, polyurethane foam. The investigation also involved the development and study of alternative foam materials having enhanced electrical and charge dissipation characteristics as a means of eliminating the electrostatic hazards during refueling. (Author)
In Vivo Imaging With Two-Photon Microscope For Assessing Tumor Selective Binding Of Anti-CD137 Switch Antibody (2021)

Abstract STA551, a novel anti-CD137 switch antibody, binds to CD137 in an extracellular ATP (exATP) concentration dependent manner. Although STA551 was assumed to show higher target binding in tumor than normal tissues, quantitative detection of the target binding of switch antibody in vivo is technically challenging. In this study, we investigated the target binding of STA551 in vivo using intravital imaging with two-photon microscopy. Tumor-bearing human CD137 knock-in mice were intravenously administered 1 mg/kg of fluorescent-labeled antibodies at day 0 and 3. Flow cytometry analysis of antibody-binding cells and intravital imaging using two-photon microscopy was conducted at day4. Higher CD137 expression in tumor than spleen was detected by flow cytometry analysis, and T cells and NK cells were major CD137 expressing cells. In the intravital imaging experiment, conventional and switch anti-CD137 antibody showed binding in tumor. However, in spleen, the fluorescence of switch antibody was much weaker than conventional anti-CD137 antibody and comparable with isotype control. In conclusion, we could assess switch antibody biodistribution in vivo through intravital imaging with two-photon microscopy. These results suggested that the tumor selective binding of STA551 leads to a wide therapeutic window and potent antitumor efficacy without systemic immune activation.
A Numerical Simulation on the Development of Cloud (1992)

Development of cumulus is studied by numerically integrating the equation of motion equations of conservation for water vapor mixing ratio, and the thermodynamic energy equuation. We use the terrain-following coordinate system called z'-coordinate system, in which we can easily treat any calculation domain with terrain configuration such as mountains. The model domain of calculation is restricted vertically to 4.Skin and horizontally to 100 km, has a bell-type mountain in the centeral part. Four cases are considered, one in a neutral environment, second in a slightly stable environment, third in a environment decreasing water content with low value of initial water vapor mixing ratio, the fourth in a case with higher vapor gradient. The more the atmosphere is unstable, the more cumulus develops easily and the more water vapors is abundant, the more cumulus develops easily too. More detailed cloud microphysics parameterizations and wet deposition must be conridered to use in air pollutants prediction model. KEY WORDS : cumulus, cloud water, stability, mixing ratio
Synthesis and characterization of sulfonated poly(phthalazinone ether sulfone ketone) for ultrafiltration and nanofiltration membranes (2000)

Modification of poly(phthalazinone ether sulfone ketone) (PPESK) by sulfonation with concentrated or fuming sulfuric acid as sulfonation agents was carried out to prepare membrane materials with increased hydrophilicity and potentially increased fouling resistance. Sulfonated PPESK (SPPESK) copolymers, with a degree of sulfonation ranging from 10–300%, were prepared and characterized. Factors affecting the sulfonation reaction were studied, and reaction conditions for the preparation of SPPESK with different degrees of sulfonation were determined. Compared with the properties of PPESK, the hydrophilicity of SPPESK was increased, as shown by a reduced contact angle with water. The glass transition temperature was increased from 278°C (PPESK) to a maximum of 323°C for the highly sulfonated derivative, due to the strong polarity of SO3H and hydrogen bonding. Ultrafiltration membranes prepared with PPESK and SPPESK were compared. For a SPPESK asymmetric membrane, the PEG12000 rejection was 98% and the water flux was 876 kg · m−2 · h−1. SPPESK/PPESK composite nanofiltration membranes were also prepared and were shown to have short-term operational stability up to 120°C. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 79: 1685–1692, 2001
Excitation-induced germanium quantum dot formation on Si(100)-(2×1) (2010)

The effect of nanosecond pulsed laser excitation on the self-assembly of Ge quantum dots grown by pulsed laser deposition on Si(100)-(2×1) was studied. In situ reflection high-energy electron diffraction and ex situ atomic force microscopy were used to probe the quantum dot structure and morphology. At room temperature, applying the excitation laser decreased the surface roughness of the grown Ge film. With surface electronic excitation, crystalline Ge quantum dots were formed at 250 °C, a temperature too low for their formation without excitation. At a substrate temperature of 390 °C, electronic excitation during growth was found to improve the quantum dot crystalline quality, change their morphology, and decrease their size distribution almost by half. A purely electronic mechanism of enhanced surface hopping of the Ge adatoms is proposed.
Association of mutant spectra of SARS-CoV-2 with COVID-19 disease severity (2022)

Introduction: Replication of RNA virus, as SARS-CoV-2, leads to composition of sequences which are at different frequencies in the infected host. Minority variants present in mutant spectra can have an epidemiological importance generating more infectious variants, resistance to vaccines and drugs and affecting to the resolution of the disease. How different are the mutant spectra of virus isolated from patients with different disease outcome has not yet been studied. Material and Methods: To approach this question, 30 patients infected with SARS-CoV-2 during the first pandemic wave in Hospital Universitario Fundación Jiménez Díaz, Madrid, Spain were classified as mild, moderate and exitus according to the disease severity of COVID-19. RNA presents in the nasopharyngeal swabs of these patients was extracted. Four amplicons of nsp12 (polymerase) and two amplicons of spike (S) coding regions were amplified. PCR products were sequenced by ultra-deep sequencing (UDS) using MiSeq platform (Illumina). Sequences were analyzed with two pipelines and differences between the mutant spectra of each virus were determined. Possible structural and functional alterations of mutations detected have been studied. Results: Most of the substitutions were found at frequencies between 0.5% to 30% in the mutant spectra. The number of mutations was significantly higher in patients with mild symptoms. The analysis of the mutant spectra resulted in a higher number of transitions and non-synonymous substitutions for in all COVID-19 categories. A major complexity of mutant spectra of virus isolated from mild patients were reflected in the study of several diversity indices, with significant statistical differences between COVID-19 categories. Structural analysis of nsp12 and spike substitutions, detected in the mutant spectra, showed possible alterations in the structure or function of the proteins. Conclusion: The study of mutant spectra shows a positive association between the number of point mutations and the complexity of mutant spectra with a mild disease outcome. Possible models for this association are discussed. Structural and functional alterations are under study right now.
Effect of a complementary horse feed on nervous horse behaviour (2011)

Master CALM’ horse feed reduced intensity of behavioural responses to startle and novelty reactions. Moreover, feeding horses with Master CALM’ influenced heart rate parameters. The lower maximal heart rates and weaker increase percentages indicated a reduction of stress level. Horses were less excited and quickly calmed down after exposure to fearful situations.
Prospective Audit to Study urokinaSe use to restore Patency in Occluded centRal venous caTheters (PASSPORT 1) (2019)

In this study, urokinase was safe and efficacious; there was no difference between dwell and push locks. There was some benefit with high-dose infusion of urokinase compared to the dwell and push lock.
Combined cervical swab and urine specimens for PCR diagnosis of genital Chlamydia trachomatis infection (2000)

Combining a cervical swab with a urine specimen is acceptable for PCR testing for genital C trachomatis infection, and has the potential to increase further the cost effectiveness of DNA based screening for C trachomatis genital infection.
The DPSM method and its application to NDE problems with interfaces modelling (2007)

This paper deals with the new results of the distributed point source method (DPSM). This method has been first developed at the SATIE lab, located at the École Normale Supérieure of Cachan, France. This method now allows the modelling of interactions between an incident wave and an obstacle. It can be applied in the non destructive testing area, using eddy currents or ultrasonic waves.
Resolving Object and Attribute Coreference in Opinion Mining (2010)

Coreference resolution is a classic NLP problem and has been studied extensively by many researchers. Most existing studies, however, are generic in the sense that they are not focused on any specific text. In the past few years, opinion mining became a popular topic of research because of a wide range of applications. However, limited work has been done on coreference resolution in opinionated text. In this paper, we deal with object and attribute coreference resolution. Such coreference resolutions are important because without solving it a great deal of opinion information will be lost, and opinions may be assigned to wrong entities. We show that some important features related to opinions can be exploited to perform the task more accurately. Experimental results using blog posts demonstrate the effectiveness of the technique. 1
Identifying Substrates for Endothelium-Specific Tie-2 Receptor Tyrosine Kinase from Phage-Displayed Peptide Libraries for High Throughput Screening (2001)

The peptide substrate specificity of Tie-2 was probed using the phage display method in order to identify efficient substrate for high throughput screening. Two random peptide libraries, pGWX3YX4 and pGWX4YX4, were constructed, in which all twenty amino acid residues were represented at the X positions flanking the fixed tyrosine residue Y. A fusion protein of GST and the catalytic domain of human Tie-2 was used to perform the phage phosphorylation. The phosphorylated phage particles were enriched by panning over immobilized anti-phosphotyrosine antibody pY20 for a total of 5 rounds. Four phage clones (3T61, 3T68, C1-90 and D1-15) that express a peptide sequence that can be phosphorylated by the recombinant catalytic domain of human Tie-2 were identified. Synthetic peptides made according to the sequences of the 4 selected clones from the two libraries, which had widely different sequences, were active substrates of Tie-2. Kinetic analysis revealed that D1-15 had the best catalytic efficiency with a k(cat)/K(m) of 5.9x10(4) M(-1) s(-1). Three high throughput screening assay formats, dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA), radioactive plate binding (RPB) and time-resolved fluorescent resonance energy transfer (TR-FRET) were developed to assess the suitability of these phage display selected peptides in screening Tie-2 inhibitors. Three out of four peptides were functional in the DELFIA assay and D1-15 was functional in the TR-FRET assay.
Nucleotide Sequence and Phylogenetic Analysis of Long Terminal Repeats of Human Endogenous Retrovirus K Family (HERV-K) on Human Chromosomes (2000)

It has been suggested that human endogenous retroviruses K family (HERV-K) has a role in disease, and solitary long terminal repeats (LTRs) of HERV-K have been potentially capable of affecting the expression of closely located genes. Using the human monochromosomes 8, 9, 17, and 18, with specific PCR primers, we identified thirty-four sequences of new HERV-K LTRs. Those LTR elements were analyzed phylogenetically with the human-specific HERV-K LTRs using neighbor-joining and maximum parsimony methods. Clones HKL8-5, HKL9-5, and HKL9-8 are related by more than 99% homology with the human-specific HERV-K LTRs. The HKL9-5 clone on chromosome 9 was 100% identical with the sequences of human-specific LTR, AC002400, on chromosome 16. The findings suggest that there has been recent proliferation, transposition, or chromosomal translocation of HERV-K LTR elements on human chromosomes.
Performance Analysis of IEEE 802.11p Preamble Insertion in C-V2X Sidelink Signals for Co-Channel Coexistence (2022)

Spectrum scarcity is one of the main challenges of future wireless technologies. When looking at vehicle-to-everything (V2X), this is amplified as spectrum sharing could impact road safety and traffic efficiency. It is therefore of particular importance to study solutions that allow the coexistence, in the same geographical area and in the same channels, of what are today the main V2X access technologies, namely IEEE 802.11p and sidelink LTE-V2X Mode 4. In this work, in addition to investigating the impact of the reciprocal interference, which we demonstrate to have a strong impact especially on the first and in congested channel conditions, a mitigation solution is extensively studied, which is based on the insertion of the IEEE 802.11p preamble at the beginning of the LTE-V2X sidelink transmission. The proposal, which is also under discussion within the standardization bodies, requires no modifications to the IEEE 802.11p protocol stack and minor changes to LTE-V2X sidelink. This solution is directly applicable to upcoming IEEE 802.11bd and extendable to NR-V2X sidelink. The paper shows, through analysis and simulations in free-flow and dense scenarios, that the proposal allows for a mitigation of collisions caused by co-channel coexistence under low to high-load channel conditions and that the improvement is also granted in congested cases when combined with additional countermeasures. Regarding the latter aspect, in particular, different approaches are compared, demonstrating that acting on the congestion control mechanisms is a simple but effective solution.
Supplementary Figures S1-7 and Tables S1-2 from Urokinase Receptor Promotes Skin Tumor Formation by Preventing Epithelial Cell Activation of Notch1 (2023)

&lt;p&gt;This file include Supplementary Figures S1-7 and Tables S1-2. Figure S1. Inflammation and neo-angiogenesis are not impaired in TPA-treated uPAR-/- skin. Figure S2. Distinct stages of hair cycling in uPAR+/+ and uPAR-/- littermates. Figure S3. FACS isolation of K14-GFP-actin/uPAR+/+ and K14-GFP-actin/uPAR-/- mouse epidermal cell sub-populations. Figure S4. Long-term growth of FACS-sorted epidermal cell subpopulations. Figure S5. Determination of "differentiated" and "proliferating" human Keratoacanthomas. Figure S6. uPAR promotes skin epithelial cell response to a proliferative stimulus by TACE-dependent Notch1 activation. Figure S7. uPAR sequesters the shedding activity of TACE in lipid rafts, thus inhibiting differentiation of primary keratinocytes. Table S1. Estimates, corresponding standard errors and P values of the fitted zero-inflated regression models. Table S2. Number of uPAR+/+ and uPAR-/- hair follicles/section in the telogen, anagen, and catagen stages.&lt;/p&gt;
Abstract 3381: Identifying tumor antigen-specific CD4+ T cell dysfunctional states by single cell transcriptomics in immunotherapy-treated cancer patients (2020)

Abstract Dysfunctional T cell states resulting from continuous exposure to self shared tumor antigens or neoantigens are key to understanding immune mechanisms that prevent proper antitumor immunity. We previously showed that repeated administration of recombinant tumor antigen vaccines without immunological adjuvant emulates a tolerized-like immune response that cannot be rescued even after in vivo re-challenge with adjuvant. We used bulk and single-cell RNA-seq of purified antigen-specific CD4+ T-cells (i.e., the orchestrator of immunity) from 12 ovarian and non-small cell lung cancer patients receiving NY-ESO-1 or MAGE-A3 vaccines with or without adjuvant, over a period of 13 weeks. First, using bulk RNA-seq data, we derived novel temporal, tolerization CD4+ T cell expression and clonotype evolution signatures using differential expression and VDJ-deconvolution analyses, highlighting significant association with cell cycle and immune activation. Then, we projected these signatures onto several recently published single-cell RNA-seq data profiling exhausted tumor-infiltrating and peripheral blood lymphocytes, finding reasonable predictive power (AUC~0.8) to classify dysfunctional T-cell states that could distinguish exhaustion from tolerance. Next, we characterized in depth the transcriptional profiles and heterogeneity of our antigen-specific CD4+ T-cells pre- and post-treatment at the single-cell level using combined scRNAseq and scTCRseq. Finally, we computationally inferred the regulatory axis of tolerized T-cells by deriving transcription factor signaling networks upstream of gene expression. In conclusion, we characterized transcriptional signatures of tumor antigen-specific CD4+ T-cells and derived a specific tolerization profile, distinguishable from exhaustion, and conserved across datasets. Through comprehensive identification of genes and pathways in a human experimental setting for induction of tolerization and exhaustion, these findings provide relevant and promising avenues to reverse dysfunctional CD4+ T cell states. Citation Format: Edgar Kozlova, Shingo Eikawa, Sacha Gnjatic, Bojan Losic. Identifying tumor antigen-specific CD4+ T cell dysfunctional states by single cell transcriptomics in immunotherapy-treated cancer patients [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 3381.
Silicon cap structure development and microassembly for forward tactile sensing in endovascular guidewire intervention (2015)

Guidewire procedure for peripheral artery disease treatment is heavily dependent on the surgeon's skills and experiences. In order to reduce such dependency, sensor-enhanced forward sensing guidewire is proposed. In this paper, we proposed a silicon cap structure that increases the robustness and efficient force transfer of the existing sensor-enhanced forward sensing guidewire. Silicon cap structure of 0.32 × 0.32 × 0.40mm has been fabricated using two masks process. Assembly of silicon structure with sensor having a 30μm overloading protection limit and resistance changes of 25kΩ with applied force ranging from 0∼40mN has been demonstrated.
The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants (2019)

Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated assembly of protein complexes, composed of both imported and locally synthesized subunits, are vital to plastid functionality. Protein biogenesis requires the action of cotranslationally acting molecular chaperones. One such chaperone is trigger factor (TF), which is known to cotranslationally bind most newly synthesized proteins in bacteria, thereby assisting their correct folding and maturation. However, how these processes are regulated in chloroplasts remains poorly understood. We report here functional investigation of chloroplast-localized TF (TIG1) in the green alga (<i>Chlamydomonas reinhardtii</i>) and the vascular land plant Arabidopsis (<i>Arabidopsis thaliana</i>). We show that chloroplastic TIG1 evolved as a specialized chaperone. Unlike other plastidic chaperones that are functionally interchangeable with their prokaryotic counterpart, TIG1 was not able to complement the broadly acting ortholog in <i>Escherichia coli.</i> Whereas general chaperone properties such as the prevention of aggregates or substrate recognition seems to be conserved between bacterial and plastidic TFs, plant TIG1s differed by associating with only a relatively small population of translating ribosomes. Furthermore, a reduction of plastidic TIG1 levels leads to deregulated protein biogenesis at the expense of increased translation, thereby disrupting the chloroplast energy household. This suggests a central role of TIG1 in protein biogenesis in the chloroplast.
Experimental Investigation of Turbulent Boundary Layer Flashback Limits for Premixed Hydrogen-Air Flames Confined in Ducts (2012)

This article discusses the results of experimental investigation of turbulent boundary layer flashback limits for premixed hydrogen-air flames confined in ducts. A tube burner experiment was set-up to double-check the findings of the channel rig. Unconfined flashback experiments were carried out by stabilizing the flame on top of the pilot burner in free atmosphere. A confined flame configuration was achieved by simply fixing a ceramic ring with a diameter higher by 4 mm on top of the pilot burner. Flashback measurements with unconfined flame holding neatly reproduced literature values for fully premixed, atmospheric H2–air mixtures and turbulent flow. The results of unconfined and confined tube burner experiments were plotted. The results showed that the drastic decrease of wall flashback stability for confined flames was the very same for both, tube and channel.
Supplemental Table 2 from A <i>BRCA1/2</i> Mutational Signature and Survival in Ovarian High-Grade Serous Carcinoma (2023)

&lt;p&gt;High grade serous carcinomas based on BRCA1/2 mutational signature and BRCA1/2 mutational status.&lt;/p&gt;
Cloning, Sequencing, and Expression of the Gene Encoding an Intracellular β-D-Xylosidase from Streptomyces thermoviolaceus OPC-520 (2001)

The intracellular beta-xylosidase was induced when Streptomyces thermoviolaceus OPC-520 was grown at 50 degrees C in a minimal medium containing xylan or xylooligosaccharides. The 82-kDa protein with beta-xylosidase activity was partially purified and its N-terminal amino acid sequence was analyzed. The gene encoding the enzyme was cloned, sequenced, and expressed in Escherichia coli. The bxlA gene consists of a 2,100-bp open reading frame encoding 770 amino acids. The deduced amino acid sequence of the bxlA gene product had significant similarity with beta-xylosidases classified into family 3 of glycosyl hydrolases. The bxlA gene was expressed in E. coli, and the recombinant protein was purified to homogeneity. The enzyme was a monomer with a molecular mass of 82 kDa. The purified enzyme showed hydrolytic activity towards only p-nitrophenyl-beta-D-xylopyranoside among the synthetic glycosides tested. Thin-layer chromatography analysis showed that the enzyme is an exo-type enzyme that hydrolyze xylooligosaccharides, but had no activity toward xylan. High activity against pNPX occurred in the pH range 6.0-7.0 and temperature range 40-50 degrees C.