gebhart/patrepeval huggingface dataset:
The ethylene signal transduction pathway inArabidopsis (1997)

The gaseous hormone ethylene is an important regulator of plant growth and development. Using a simple response of etiolated seedlings to ethylene as a genetic screen, genes involved in ethylene signal transduction have been identified in Arabidopsis. Analysis of two of these genes that have been cloned reveals that ethylene signalling involves a combination of a protein (ETR1) with similarity to bacterial histidine kinases and a protein (CTR1) with similarity to Raf-1, a protein kinase involved in multiple signalling cascades in eukaryotic cells. Several lines of investigation provide compelling evidence that ETR1 encodes an ethylene receptor. For the first time there is a glimpse of the molecular circuitry underlying the signal transduction pathway for a plant hormone.
Inside Visual C (1994)

This guide brings together three topics in programming: Windows, application frameworks and C++. Application framework programming, a widely accepted approach on the Macintosh platform, represents a logical, time-saving and next step in programming for Windows, providing an examination of the technology and its implementation. The main part of the book is a complete tutorial for programming in Windows with C++ elements, class libraries and application frameworks. The programming language is Microsoft C/C++ Version 7.
Transcriptional Repression of Protein Kinase Cα via Sp1 by Wild Type p53 Is Involved in Inhibition of Multidrug Resistance 1 P-Glycoprotein Phosphorylation (2004)

The protein kinase C (PKC) family consists of serine/threonine protein kinases that play important roles in signal transduction, cell proliferation, and tumor formation. Recent studies found that PKCs are commonly overexpressed in human tumors, including soft tissue sarcoma (STS). Overexpression of PKCs contributes to invasion and migration of tumor cells and induction of angiogenesis. PKC can also phosphorylate the multidrug resistance (MDR) gene-encoded P-glycoprotein and induce MDR phenotype. Our previous studies showed that mutation of p53 enhanced STS metastasis and mediated the MDR phenotype. Restoring wild type (WT) p53 in STS cells containing mutant p53 sensitized the cells to chemotherapy. In the present study, we found that PKCalpha protein expression is inhibited by WT p53 partly due to reduced PKCalpha mRNA expression in STS cells, but p53 does not affect PKCalpha mRNA stability. Deletion and mutation analysis of the PKCalpha promoter fused to the luciferase reporter gene identified a Sp1 binding site (-244/-234) in the PKCalpha promoter that is required for p53-mediated inhibition of PKCalpha promoter activity. More importantly, PKCalpha phosphorylates and activates MDR1 P-glycoprotein, whereas inhibition of PKCalpha by p53 leads to decreased MDR1 phosphorylation in STS cells, which sensitizes STS cells to chemotherapeutic agents. These data indicate that WT p53 may resensitize STS to chemotherapeutic agents by reducing MDR1 phosphorylation via transcriptional repression of PKCalpha expression. Thus, molecular-based therapies targeting mutant p53 and PKCalpha may be an effective new strategy to improve chemotherapeutic efficacy in STS.
Semisynthetic pathways for the preparation of metabolites of secoiridoids of Olea europea (2014)

Dottorato di Ricerca in Metodologie per lo sviluppo di molecole d'interesse farmacologico, Ciclo XXII.a.a. 2009
R-Type Calcium Channels Are Crucial for Semaphorin 3A–Induced DRG Axon Growth Cone Collapse (2014)

Semaphorin 3A (Sema3A) is a secreted protein involved in axon path-finding during nervous system development. Calcium signaling plays an important role during axonal growth in response to different guidance cues; however it remains unclear whether this is also the case for Sema3A. In this study we used intracellular calcium imaging to figure out whether Sema3A-induced growth cone collapse is a Ca2+ dependent process. Intracellular Ca2+ imaging results using Fura-2 AM showed Ca2+ increase in E15 mice dorsal root ganglia neurons upon Sema3A treatment. Consequently we analyzed Sema3A effect on growth cones after blocking or modifying intracellular and extracellular Ca2+ channels that are expressed in E15 mouse embryos. Our results demonstrate that Sema3A increased growth cone collapse rate is blocked by the non-selective R- and T- type Ca2+ channel blocker NiCl2 and by the selective R-type Ca2+ channel blocker SNX482. These Ca2+ channel blockers consistently decreased the Sema3A-induced intracellular Ca2+ concentration elevation. Overall, our results demonstrate that Sema3A-induced growth cone collapses are intimately related with increase in intracellular calcium concentration mediated by R-type calcium channels.
The Hi-NOON neural simulator and its applications to animal, animat and humanoid studies (2000)

This paper describes the Hi-NOON neural simulator, originally conceived as a general-purpose, object-oriented software system for the simulation of small systems of biological neurons, as an aid to the study of links between neurophysiology and behaviour in lower animals. As such, the artificial neurons employed are spiking in nature: to effect an appropriate compromise between computational complexity and biological realism, modeling was at the transmembrane potential level of abstraction. Further, since real neural systems incorporate different types of neurons specialized to some what different functions, the software was written to accommodate a non-homogeneous population of neurons. Hi-NOON has been used in animat (crick et phono-taxis) and biologically-based robot studies. In particular, it was employed to implement the nervous system of our ARBIB robot. A simple model of synaptogenesis has been added so improving the stability of its learning in the light of the stability-plasticity dilemma, and as a mechanism for long-term memory. The efficacy of the simulator is illustrated with respect to some recent applications to situated systems studies. Now that Hi-NOON has been expanded to simulate large nervous systems in a concurrent environment, it can be applied to humanoid robotics in the future.
Learning to separate vocals from polyphonic mixtures via ensemble methods and structured output prediction (2016)

Separating the singing from a polyphonic mixed audio signal is a challenging but important task, with a wide range of applications across the music industry and music informatics research. Various methods have been devised over the years, ranging from Deep Learning approaches to dedicated ad hoc solutions. In this paper, we present a novel machine learning method for the task, using a Conditional Random Field (CRF) approach for structured output prediction. We exploit the diversity of previously proposed approaches by using their predictions as input features to our method - thus effectively developing an ensemble method. Our empirical results demonstrate the potential of integrating predictions from different previously-proposed methods into one ensemble method, and additionally show that CRF models with larger complexities generally lead to superior performance.
Selection of appropriate candidates for a type position using C4.5 decision tree (2017)

Recruitment and selection of new employees rank to the important processes of human potential management and development. Especially the process of employee selection prepares proper conditions for a successful work performance and decides on a future progress-ability of the organizations. In a unique sector of private security, the precise realization of employee selection can solve one of the most frequent problem of the private security organizations: high fluctuation/employee turnover. The paper focuses on an experimental possibility to assign systematically, and with the high measure of exactness, the required competences of candidates to the specification of clients' protected interests or objects. In presented experiment study, built on the basis of benchmarking, the paper presents the concept of well-known decision trees to choose best candidates. Demonstration of proposed system and method is done by public available data, where we achieved 97.27% accuracy of classification.
Erratum: Manipulating quantum materials with quantum light [Phys. Rev. B 99, 085116 (2019)] (2019)

Received 7 March 2019DOI:https://doi.org/10.1103/PhysRevB.99.099907©2019 American Physical SocietyPhysics Subject Headings (PhySH)Research AreasFermionsLight-matter interactionMagnetic interactionsMagnetic susceptibilityNonlinear opticsOptical conductivityPolaritonsPhysical SystemsMott insulatorsQuantum cavitiesTechniquesCavity methodsHubbard modelNonperturbative methodsPerturbation theoryCondensed Matter, Materials & Applied PhysicsAtomic, Molecular & Optical
Ontogeny of 6-keto-PGF1 alpha synthesis in rabbit aorta and the effect of premature weaning (1987)

A systematic study of the ontogeny of aortic 6-ketoprostaglandin F1 alpha synthesis from birth to adult life and the effect of premature weaning on this process was investigated in rabbits. Prostacyclin (PGI2) synthesis by both endogenous and exogenous arachidonic acid and its relation to aortic arachidonic acid content was determined. It was found that 1) PGI2 synthesis from endogenous arachidonic acid increased with age, whereas 2) PGI2 synthesis from exogenous arachidonic acid decreased. This correlated with a decrease in the incorporation of [14C]arachidonic acid into phospholipids with age. Aortic arachidonic acid concentration did not change from birth until 3 wk of life but increased markedly by 5 wk of age. Premature weaning caused a decrease in the synthesis of aortic PGI2 and in aortic arachidonic acid concentration initially, but the changes did not persist in later life. These studies suggest that the utilization of exogenous arachidonic acid by aorta decreases after birth perhaps due to maturity of the enzyme systems that synthesize and utilize endogenous substrates.
Seismic Surveillance and Earthquake Monitoring in Italy (2021)

Abstract The Istituto Nazionale di Geofisica e Vulcanologia (INGV) is an Italian research institution with focus on earth sciences. Moreover, the INGV is the operational center for seismic surveillance and earthquake monitoring in Italy and is a part of the civil protection system as a center of expertise on seismic, volcanic, and tsunami risks.INGV operates the Italian National Seismic Network and other networks at national scale and is a primary node of the European Integrated Data Archive for archiving and distributing strong-motion and weak-motion seismic recordings. In the control room in Rome, INGV staff performs seismic surveillance and tsunami warning services; in Catania and Naples, the control rooms are devoted to volcanic surveillance. Volcano monitoring includes locating earthquakes in the regions around the Sicilian (Etna, Eolian Islands, and Pantelleria) and the Campanian (Vesuvius, Campi Fregrei, and Ischia) active volcanoes. The tsunami warning is based on earthquake location and magnitude (M) evaluation for moderate to large events in the Mediterranean region and also around the world. The technologists of the institute tuned the data acquisition system to accomplish, in near real time, automatic earthquake detection, hypocenter and magnitude determination, and evaluation of several seismological products (e.g., moment tensors and ShakeMaps). Database archiving of all parametric results is closely linked to the existing procedures of the INGV seismic surveillance environment and surveillance procedures. Earthquake information is routinely revised by the analysts of the Italian seismic bulletin. INGV provides earthquake information to the Department of Civil Protection (Dipartimento di Protezione Civile) to the scientific community and to the public through the web and social media. We aim at illustrating different aspects of earthquake monitoring at INGV: (1) network operations; (2) organizational structure and the hardware and software used; and (3) communication, including recent developments and planned improvements.
Modeling and Analysis of Engine Torque Modulation for Shift Quality Improvement (2006)

&lt;div class="htmlview paragraph"&gt;An engine torque modulation methodology is developed for use in engines equipped with electronic throttle control, ETC. It is shown through simulation that an engine with ETC is capable of following a transmission torque modulation command that includes a linearly increasing torque during the torque phase, a torque reduction during the inertia phase and a post-shift torque increase. It is also shown that such a strategy can be used to minimize the transmission output torque variation during a shift. The impact of engine indicated mean effective pressure, IMEP, variation on vehicle longitudinal acceleration is analyzed using Monte-Carlo simulation. It is shown that the IMEP induced variation in the vehicle acceleration is largely due to end of shift engine torque output timing errors. It is then recommended that during a shift the engine IMEP variation be held to a one-sigma variation of three percent or less. Appendices A and B summarize the derivation of a low order planetary gear driveline model that is used to illustrate the torque relationships and dynamics associated with shifting an automatic transmission.&lt;/div&gt;
Green light emission from InGaN multiple quantum wells grown on GaN pyramidal stripes using selective area epitaxy (2008)

Selective area epitaxy has been used to grow pyramidal GaN stripes, followed by InGaN multiple quantum well (MQW) structures, in order to produce long-wavelength green light emission. Stripes oriented along ⟨112¯0⟩ produce smooth {11¯01} sidewall facets. The room-temperature optical properties are investigated by cathodoluminescence spectroscopy using a scanning electron microscope. MQWs grown in unmasked reference regions exhibit emission at 450 nm. The stripe sidewalls emit light with peak wavelength of 500 nm with consistent linewidth and intensity. The stripe ridge emits light with peak intensity at wavelength of ∼550 nm. Based on the spatial extent of the 550 nm emission, the ridge is estimated to be ∼250 nm wide. The large redshift is produced by the enhanced presence of indium species due to lateral vapor diffusion and surface migration in selective area epitaxy.
Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module (2016)

Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues.
Phosphoinositide-3-kinases p110α and p110β mediate S phase entry in astroglial cells in the marginal zone of rat neocortex (2013)

In cells cultured from neocortex of newborn rats, phosphoinositide-3-kinases of class I regulate the DNA synthesis in a subgroup of astroglial cells. We have studied the location of these cells as well as the kinase isoforms which facilitate the S phase entry. Using dominant negative (dn) isoforms as well as selective pharmacological inhibitors we quantified S phase entry by nuclear labeling with bromodeoxyuridine (BrdU). Only in astroglial cells harvested from the marginal zone (MZ) of the neocortex inhibition of phosphoinositide-3-kinases reduced the nuclear labeling with BrdU, indicating that neocortical astroglial cells differ in the regulation of proliferation. The two kinase isoforms p110α and p110β were essential for S phase entry. p110α diminished the level of the p27(Kip1) which inactivates the complex of cyclin E and CDK2 necessary for entry into the S phase. p110β phosphorylated and inhibited glycogen synthase kinase-3β which can prevent S-phase entry. Taken together, both isoforms mediated S phase in a subgroup of neocortical astroglial cells and acted via distinct pathways.
Cardiac Involvement in a Patient With Coronavirus Disease 2019 (COVID-19) (2020)

<h3>Importance</h3> Virus infection has been widely described as one of the most common causes of myocarditis. However, less is known about the cardiac involvement as a complication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. <h3>Objective</h3> To describe the presentation of acute myocardial inflammation in a patient with coronavirus disease 2019 (COVID-19) who recovered from the influenzalike syndrome and developed fatigue and signs and symptoms of heart failure a week after upper respiratory tract symptoms. <h3>Design, Setting, and Participant</h3> This case report describes an otherwise healthy 53-year-old woman who tested positive for COVID-19 and was admitted to the cardiac care unit in March 2020 for acute myopericarditis with systolic dysfunction, confirmed on cardiac magnetic resonance imaging, the week after onset of fever and dry cough due to COVID-19. The patient did not show any respiratory involvement during the clinical course. <h3>Exposure</h3> Cardiac involvement with COVID-19. <h3>Main Outcomes and Measures</h3> Detection of cardiac involvement with an increase in levels of N-terminal pro–brain natriuretic peptide (NT-proBNP) and high-sensitivity troponin T, echocardiography changes, and diffuse biventricular myocardial edema and late gadolinium enhancement on cardiac magnetic resonance imaging. <h3>Results</h3> An otherwise healthy 53-year-old white woman presented to the emergency department with severe fatigue. She described fever and dry cough the week before. She was afebrile but hypotensive; electrocardiography showed diffuse ST elevation, and elevated high-sensitivity troponin T and NT-proBNP levels were detected. Findings on chest radiography were normal. There was no evidence of obstructive coronary disease on coronary angiography. Based on the COVID-19 outbreak, a nasopharyngeal swab was performed, with a positive result for SARS-CoV-2 on real-time reverse transcriptase–polymerase chain reaction assay. Cardiac magnetic resonance imaging showed increased wall thickness with diffuse biventricular hypokinesis, especially in the apical segments, and severe left ventricular dysfunction (left ventricular ejection fraction of 35%). Short tau inversion recovery and T2-mapping sequences showed marked biventricular myocardial interstitial edema, and there was also diffuse late gadolinium enhancement involving the entire biventricular wall. There was a circumferential pericardial effusion that was most notable around the right cardiac chambers. These findings were all consistent with acute myopericarditis. She was treated with dobutamine, antiviral drugs (lopinavir/ritonavir), steroids, chloroquine, and medical treatment for heart failure, with progressive clinical and instrumental stabilization. <h3>Conclusions and Relevance</h3> This case highlights cardiac involvement as a complication associated with COVID-19, even without symptoms and signs of interstitial pneumonia.
Mantle Cell Lymphoma Can Be Cured by Intensive Immunochemotherapy with In-Vivo Purged Stem-Cell Support; Final Report of the Nordic Lymphoma Group MCL2 Study. (2007)

Abstract Mantle cell lymphoma (MCL) is considered incurable, with a median survival of 4 years. Intensive immunochemotherapy and autologous stem-cell (ASC) support has appeared promising in small patient cohorts, but has not been tested in large, consecutive series. Here we report the final results of the 2nd Nordic MCL (MCL2) trial after a median of 3 years follow-up from study entry. Methods: This unrandomized phase-II trial included 159 untreated patients younger than 66 years, 84% stage IV, 128 with classical, 31 with blastoid/pleomorphic cytology. Following 6 cycles of intensive induction immunochemotherapy with alternating cycles of rituximab (R) + maxi-CHOP and R+ high-dose AraC, responders received BEAM/BEAC with in-vivo purged (R) ASC support. Results: 153 patients (96%) responded to induction therapy with CR in 55% and PR in 41%. The 5-year event-free (EFS) and overall survival (OAS) are 63% and 74% respectively on intention-to-treat, and the 144 (91%) responders who completed treatment had 72% 5-year response duration, with plateaus emerging in all three curves at these levels. Figure Figure There were 6 treatment-related deaths (3,8%). Of 77 patients with available primers, 90% had become PCR-negative two months posttransplant; those who remained PCR-negative more than 1 year posttransplant had a significantly longer clinical response duration than patients who did not (P&lt;0.0001). Of 42 stem-cell products assessed 88% were PCR-negative as compared to only 12% in the MCL1 study (P&lt;0.001). In a multivariate analysis including age, sex, international prognostic index (IPI), cytological variants and Ki-67 proliferation index, only IPI and Ki-67 were independent predictors of EFS and response duration respectively, and only IPI and cytological variant of OAS. Compared to the 41 patients of 1st Nordic MCL study who received 4 cycles of maxi-CHOP without rituximab before BEAM or BEAC + ASCT (1), the OAS, EFS and response duration were highly significantly increased. Conclusion: The demonstration of long-term event-free survival in a large, consecutive prospective series now for the first time indicates that intensive immunochemotherapy including AraC and Rituximab with in-vivo purged stem-cell support may cure mantle cell lymphoma.
Storage of red blood cells with improved maintenance of 2,3‐bisphosphoglycerate (2006)

Improvements in the maintenance of 2,3-DPG were achieved with 175 mL of a chloride-free storage solution with familiar additives at nontoxic concentrations to increase pHi. Adding, instead, 400 mL of storage solution followed by the removal of 300 mL reduced the chloride concentration, increasing the pHi and extending the maintenance of 2,3-DPG to 4 weeks.
Observation of localisation and interaction corrections to the conductivity and thermopower of a two-dimensional electron gas (1989)

The authors have measured the conductivity and thermopower of silicon-on-sapphire MOSFETs at low temperatures as a function of perpendicular and parallel magnetic fields. The magnitude of the thermopower was found to decrease for perpendicular fields up to about 1 T. An explanation of this effect is offered in terms of the quenching of weak localisation and the dominance of phonon-drag effects. At higher fields, both perpendicular and parallel, the thermopower was found to increase again, which they attribute to enhancement of electron-electron interactions by the field.