gebhart/patrepeval huggingface dataset:
Nϵ‐Carboxymethyllysine Increases the Expression of miR‐103/143 and Enhances Lipid Accumulation in 3T3‐L1 Cells (2016)

Advanced glycation endproducts, formed in vivo, but also by the Maillard reaction upon thermal treatment of foods, have been associated with the progression of pathological conditions such as diabetes mellitus. In addition to the accumulation with age, exogenous AGEs are introduced into the circulation from dietary sources. In this study, we investigated the effects of addition of free N(ϵ) -carboxymethyllysine (CML), a well-characterized product of the Maillard reaction, on adipogenesis in 3T3-L1 preadipocytes. Treatment with 5, 50, or 500 μM CML resulted in increased lipid accumulation to similar extents, by 11.5 ± 12.6%, 12.9 ± 8.6%, and 12.8 ± 8.5%, respectively. Long-term treatment with 500 μM CML during adipogenesis resulted in increases in miR-103 and miR-143 levels, two miRNAs described to be involved in impaired glucose homeostasis and increased lipid accumulation. Furthermore, the expression of genes associated with these miRNAs, consisting of Akt1, PI3k, and Cav1 was regulated by CML. Short-term treatment of mature 3T3-L1 adipocytes with CML resulted in decreased basal glucose uptake. These results, indicate that the addition of protein-free CML to 3T3-L1 cells influence parameters associated with adipogenesis and glucose homeostasis at transcriptional, and functional level; this indicates that free CML derived from exogenous sources, in addition to protein-bound CML may be relevant in this context. J. Cell. Biochem. 117: 2413-2422, 2016. © 2016 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals, Inc.
Interactive Sculpting of Digital Faces Using an Anatomical Modeling Paradigm (2020)

Abstract Digitally sculpting 3D human faces is a very challenging task. It typically requires either 1) highly‐skilled artists using complex software packages for high quality results, or 2) highly‐constrained simple interfaces for consumer‐level avatar creation, such as in game engines. We propose a novel interactive method for the creation of digital faces that is simple and intuitive to use, even for novice users, while consistently producing plausible 3D face geometry, and allowing editing freedom beyond traditional video game avatar creation. At the core of our system lies a specialized anatomical local face model (ALM), which is constructed from a dataset of several hundred 3D face scans. User edits are propagated to constraints for an optimization of our data‐driven ALM model, ensuring the resulting face remains plausible even for simple edits like clicking and dragging surface points. We show how several natural interaction methods can be implemented in our framework, including direct control of the surface, indirect control of semantic features like age, ethnicity, gender, and BMI, as well as indirect control through manipulating the underlying bony structures. The result is a simple new method for creating digital human faces, for artists and novice users alike. Our method is attractive for low‐budget VFX and animation productions, and our anatomical modeling paradigm can complement traditional game engine avatar design packages.
Metabolic engineering of the initial stages of xylose catabolism in yeast for the purpose of constructing efficient producers of ethanol from lignocellulosics (2008)

Plant biomass possesses huge potential as a source for the production of biofuels. Glucose and the five-carbon sugar xylose are the principal constituents of biomass. The yeast Saccharomyces cerevisiae, which is used for industrial production of ethanol from glucose is not capable of fermenting xylose. Thus, it is necessary to find in Nature or to create microorganisms capable of achieving efficient fermentation of glucose and xylose, as a means of achieving economically feasible biomass conversion into ethanol. Active fermentation of xylose may be achieved if the initial stages of metabolism are efficiently performed [1]. In this review, the enzymes of the initial stages of xylose metabolism in yeast (xylose reductase, xylitol dehydrogenase, and xylulokinase) and bacteria (xylose isomerase and xylulokinase) are characterized. The ways for constructing yeast strains capable of achieving efficient alcoholic xylose fermentation are discussed.
Control with a Compliant Force-Torque Sensor (2012)

There are assembly tasks which require a compliant device at the end-effector since possible disturbances are beyond the bandwidth of robot control. This paper discusses a compliant force-torque sensor for assembly. Two aspects are explained in detail: Force control considering a significant force dependent displacement, and control of an end-effector with an elastic mounting during fast unconstrained motion. The latter uses an adaptive scheme which serves as a further level in a hierarchical position-based control. Experimental results are given which show the limits of industrial robots. 1
Demography of the Koronadal B?la-an: A comparative study of child survival in the Tampakan Highlands, Southern Philippines (2010)

The Tampakan Highlands of the Central Mindanao Cordillera in the Philippines have received international attention due to the discovery of a copper–gold deposit in the 1990s and subsequent minerals exploration and community development initiatives as part of the Tampakan Project. This book compares infant and child mortality parameters of indigenous B’la-an and Christian Visayan populations residing within the project area over a fifteen-year period (1983–97). In so doing, it uniquely profiles Highlands B'la-an peoples – forest slash-and-burn farmers and hunter–gatherers who have experienced little acculturation but who face potentially irrevocable changes should a large-scale mine be realised. Further, it explores the impact of maternal education and pioneering health-care efforts on infant and child survival in a politically volatile and socioeconomically disadvantaged area. These foci will be of interest to researchers and practitioners in demography, population health, biomedical sciences, social impact assessment, and anthropology, as well as Philippine, indigenous, and development studies.
Co‐presence of a point mutation and a deletion of exon 3 in the glycophorin C gene and concomitant production of a Gerbich‐related antibody (1997)

The specificity of antibody in RW's serum was serologically anti-Ge2. Two genetic events occurred in exon 3 in GYPC of RW and her compatible siblings. The exon 3 deletion confirmed a Ge:-2,-3,4 haplotype. The abnormal tryptic fragment obtained was due to the (A173-->T) base change in exon 3 that resulted in Asp58-->Val in the deduced amino acid sequence at the membrane boundary.
Real-Time Monitoring and Diagnosis of Electrical Submersible Pump (2015)

Abstract Electrical Submersible Pump (ESP) lift is extensively used in offshore production systems. Although real-time pump operating parameters and the production data are commonly available according to the recently developed digital energy technologies, the analysis of the acquired data is still insufficient to monitor, diagnose, interpret, and analyze reservoir performance, wellbore integrity, and ESP operating status and efficiency in a real-time manner. Further, the traditional ammeter card diagnosis cannot identify leakage of tubing, underperforming pump lifting, and low pump working efficiency. This paper summarizes typical characteristics of different downhole malfunctions in ESP wells, provides a novel approach to detect those problems systematically and automatically. The developed management system incorporates real-time ESP current data and interpret ammeter card by neural network analysis, which has been trained through over 900 wells. We also derived the analytical solutions for wellhead pressure buildup analysis as a supplement to neural network analysis. Consequently, this model is able to detect downhole problems that cannot be identified by ammeter card. This diagnosis model also sets thresholds for key parameters, and alarm operation team through satellite in a timely manner. This model is a reliable supplementary to the traditional ammeter card diagnosis method, and the online utility provides complement flexibility to cooperate with field operations in real-time. The early-stage identification and resolution of ESP problems can lead to a great cost-saving and less maintenance requirements owing to this intelligent system. This workflow has been successful in field trials.
Plasma enhanced atomic layer deposition of silicon nitride using neopentasilane (2015)

Progress in transistor scaling has increased the demands on the material properties of silicon nitride (SiNx) thin films used in device fabrication and at the same time placed stringent restrictions on the deposition conditions employed. Recently, low temperature plasma enhanced atomic layer deposition has emerged as a viable technique for depositing these films with a thermal budget compatible with semiconductor processing at sub-32 nm technology nodes. For these depositions, it is desirable to use precursors that are free from carbon and halogens that can incorporate into the film. Beyond this, it is necessary to develop processing schemes that minimize the wet etch rate of the film as it will be subjected to wet chemical processing in subsequent fabrication steps. In this work, the authors introduce low temperature deposition of SiNx using neopentasilane [NPS, (SiH3)4Si] in a plasma enhanced atomic layer deposition process with a direct N2 plasma. The growth with NPS is compared to a more common precursor, trisilylamine [TSA, (SiH3)3 N] at identical process conditions. The wet etch rates of the films deposited with NPS are characterized at different plasma conditions and the impact of ion energy is discussed.
Biochemical and immunological characterization of E. coli expressed 42 kDa fragment of Plasmodium vivax and P. cynomolgi bastianelli merozoite surface protein-1. (2007)

Plasmodium vivax is one of the most widely distributed human malaria parasites and due to drug-resistant strains, its incidence and prevalence has increased, thus an effective vaccine against the parasites is urgently needed. One of the major constraints in developing P. vivax vaccine is the lack of suitable in vivo models for testing the protective efficacy of the vaccine. P. vivax and P. cynomolgi bastianelli are the two closely related malaria parasites and share a similar clinical course of infection in their respective hosts. The merozoite surface protein-1 (MSP-1) of these parasites has found to be protective in a wide range of host-parasite systems. P. vivax MSP-1 is synthesized as 200 kDa polypeptide and processed just prior to merozoite release from the erythrocytes into smaller fragments. The C- terminal 42 kDa cleavage product of MSP-1 (MSP-1(42)) is present on the surface of merozoites and a major candidate for blood stage malaria vaccine. In the present study, we have biochemically and immunologically characterized the soluble and refolded 42 kDa fragment of MSP-1 of P. vivax (PvMSP-1(42)) and P. cynomolgi B (PcMSP-1(42)). SDS-PAGE analysis showed that both soluble and refolded E. coli expressed P. vivax and P. cynomolgi B MSP-1(42) proteins were homogenous in nature. The soluble and refolded MSP-1(42) antigens of both parasites showed high reactivity with protective monkey sera and conformation-specific monoclonal antibodies against P. cynomolgi B and P. vivax MSP-1(42) antigens. Immunization of BALB/c mice with these antigens resulted in the production of high titres of cross-reactive antibodies primarily against the conformational epitopes of MSP-1(42) protein. The immune sera from rhesus monkeys. immunized with soluble and refolded MSP-1(42) antigens of both parasites also showed high titered cross-reactive antibodies against MSP-1(42) conformational epitopes. These results suggested that the soluble and refolded forms of E. coli expressed P. vivax MSP-1(42) antigens were highly immunogenic and thus a viable candidate for vaccine studies.
Glucose-Based Fluorinated Surfactants as Additives for the Crystallization of Membrane Proteins: Synthesis and Preliminary Physical–Chemical and Biochemical Characterization (2021)

We report herein the synthesis of a series of fluorinated surfactants with a glucose moiety as a polar head group and whose alkyl chain was varied in length and in fluorine/hydrogen ratio. They were synthesized in two or four steps in 20 to 50% overall yields allowing gram-scale synthesis. Their solubility in water is between 0.2 and 13.8 g/L, which indicates low water solubility. Two derivatives of the series were found to form micelles in water at ∼11 mM. Their hydrophilic-lipophilic balance was determined both by Griffin's and Davies' methods; they may exhibit a "harsh" character toward membrane proteins. This, combined with their low water solubility, suggest that they could advantageously be used in detergent mixtures containing a "mild" detergent. Finally, the potency of one of the derivatives, F<sub>3</sub>H<sub>5</sub>-β-Glu, to act as an additive for the crystallization of AcrB was evaluated in detergent mixtures with <i>n</i>-dodecyl-β-d-maltopyranoside (DDM). Among the six crystallization conditions investigated, adding F<sub>3</sub>H<sub>5</sub>-β-Glu improved the crystallization for three of them, as compared to control drops without additives. Moreover, preliminary tests with other compounds of the series showed that none of them hampered crystallization and suggested improvement for three of them. These novel glucose-based fluorinated detergents should be regarded as potential additives that could be included in screening kits used in crystallization.
Effects of the two-step ageing treatment on the microstructure and properties of 7B04 alloy pre-stretched thick plates (2007)

The effects of the two-step ageing parameters (temperature and time) on the mechanical properties and electrical conductivity of 7B04 (Al-Zn-Mg-Cu) pre-stretched thick plates were studied. The results reveal that the initial T1 ageing contributes a major increase of the tensile strength, and the 0.2% proof stress value reaches 482 MPa after ageing for 7 h at 115°C. Behavioral differences in the tensile properties of the alloy after the two-step ageing treatment were less with the first-step ageing at 115°C for different time periods (7, 14, and 21 h). The effects of the second ageing parameters on the properties and microstructure of the 7B04 alloy were remarkable. TEM analysis of the samples aged at Temper I (7 h at 115°C + 12 h at 160°C) and Temper II (7 h at 115°C + 16 h at 165°C) indicates that two kinds of phases, i.e. η' and η phases, precipitate from the matrix and efficiently improve the tensile strength of the alloy, and the grain boundary precipitates are coarse and discrete. There are obvious precipitate free zones (PFZs) along the grain boundary in the microstructure of the alloy after the two-step ageing treatment.
Interaction of a fluorescent ligand with membrane-bound cholinergic receptor from Torpedo marmorata (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction of a fluorescent ligand with membrane-bound cholinergic receptor from Torpedo marmorataJonathan B. Cohen and Jean P. ChangeuxCite this: Biochemistry 1973, 12, 24, 4855–4864Publication Date (Print):November 1, 1973Publication History Published online1 May 2002Published inissue 1 November 1973https://pubs.acs.org/doi/10.1021/bi00748a008https://doi.org/10.1021/bi00748a008research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
An Efficient Boundary-Based Approach for Shape Representation (2006)

Content-based image retrieval (CBIR) has become a major area of research and received increasing attention. Shape representation and description is one of the major problems in CBIR. This paper outlines and presents a novel boundary-based approach for shape representation and description, which is capable of extracting reliable information of the object outline. Our technique is translation, scale, and rotation invariant. It works well on many types of shapes and fast enough for use in real-time. This technique produces a concise representation of the image that can be used to search an image database for a similar image
Morphometric and Quantitative Immunohistochemical Analysis of Disease-Related Changes in the Upper (Suburothelial) Lamina Propria of the Human Bladder Dome (2015)

The upper (suburothelial) lamina propria (ULP) is a distinct region in the human bladder with dense populations of interstitial cells (IC), fine vascular networks and variable development of muscularis mucosae (MM). It is more and more obvious that the ULP plays an important role in bladder physiology and bladder disease, and in the present study we have quantified changes in the cellular key players of the ULP in bladders from patients with carcinoma in situ (CIS), multiple sclerosis (MS) and bladder pain syndrome (BPS). Tissue samples for the different patient groups were obtained from radical cystectomy-specimens. Standardized immunohistochemistry with a panel of specific cell markers was used to characterise the ULP cellular structures, followed by digitalised morphometry and quantitative staining analysis. Alterations in the ULP area were most pronounced in MS bladders, but also present in BPS and CIS bladders. We observed an increased thickness and increased variability in thickness of the ULP IC area in MS and BPS bladders; a significantly increased development of MM in MS bladders; a changed organization of vascular plexuses in the lamina propria in most pathologic bladders and a changed phenotype of ULP IC: a significantly decreased expression of progesterone receptor in MS bladders and a trend towards decreased expression of alpha-smooth muscle actin in BPS bladders. We show here for the first time the presence of disease-specific changes in organisation and/or phenotype of the different key players of the ULP area in human bladder. The present findings further support the hypothesis that the ULP area is involved and altered in different bladder diseases.
A novel thrombopoietin signaling defect in polycythemia vera platelets (1998)

The pathogenesis of polycythemia vera (PV), a disease involving a multipotent hematopoietic progenitor cell, is unknown. Thrombopoietin (TPO) is a newly characterized hematopoietic growth factor which regulates the production of multipotent hematopoietic progenitor cells as well as platelets. To evaluate the possibility that an abnormality in TPO-mediated signal transduction might be involved in the pathogenesis of PV, we examined TPO-induced protein tyrosine phosphorylation using platelets as a surrogate model system. Platelets were isolated from the blood of patients with PV as well as from patients with other chronic myeloproliferative disorders and control subjects. Impaired TPO-mediated platelet protein tyrosine phosphorylation was a consistent observation in patients with PV as well as those with idiopathic myelofibrosis (IMF), in contrast to patients with essential thrombocytosis, chronic myelogenous leukemia, secondary erythrocytosis, iron deficiency anemia, hemochromatosis, or normal volunteers. Thrombin-mediated platelet protein tyrosine phosphorylation was intact in PV platelets as was expression of the appropriate tyrosine kinases and their cognate substrates. However, expression of the platelet TPO receptor, Mpl, as determined by immunoblotting, chemical crosslinking or flow cytometry was markedly reduced or absent in 34 of 34 PV patients and also in 13 of 14 IMF patients. Impaired TPO-induced protein tyrosine phosphorylation in PV and IMF platelets was uniformly associated with markedly reduced or absent expression of Mpl. We conclude that reduced expression of Mpl is a phenotypic characteristic of platelets from patients with PV and IMF. The abnormality appears to distinguish PV from other forms of erythrocytosis and may be involved in the platelet function defect associated with PV.
CHRONIC EFFECTS OF ATRAZINE ON TOLERANCE OF A BENTHIC DIATOM (1999)

The effects of prior chronic exposure to atrazine on responses to subsequent acute exposures were investigated using a common benthic diatom.Clonal, axenic cultures of Craticula cuspidata were established from the Platte River (Nebraska) and obtained from a culture collection (unlikely prior exposure to atrazine).All cultures received a chronic 67-d treatment of 1 g/L atrazine, and growth was monitored using fluorometric detection of chlorophyll a. Chronic atrazine exposure significantly reduced growth rate only during the first day of treatment (p ϭ 0.0001); no significant effect was detected throughout the remainder of the 67-d period.Following the chronic treatment, clones were exposed to six atrazine concentrations (83, 188, 402, 860, 1,782, and 3,250 g/L) to ascertain whether prior exposure influenced the tolerance of this diatom.Prior chronic exposure had a negative effect on growth following subsequent exposures to higher concentrations.A significant decline in growth was detected on days 7, 9, and 12 between previously exposed and control clones at 83 g/L of atrazine.The lack of increased tolerance in C. cuspidata after a realistic chronic exposure indicates that the levels of atrazine presently found in many lotic systems may inhibit the growth of periphyton during periods of higher pulses of atrazine characteristic of spring runoff events.