gebhart/patrepeval huggingface dataset:
The isolation,purity determination by RP-HPLC and structural elucidation of ricinine (2013)

The ricinine was isolated and purified from the Ricinus communis L..The purity(99.9%) was confirmed by RP-HPLC and the structure was characterized by the IR,1H NMR and MS.Subsequently,the optimized structure,charge distribution,molecular total energy,HOMO and LUMO orbital energy were calculated by quantum chemical method at DFT-B3LYP/6-311+G(d) basis.
Modern carbonate ooids preserve ambient aqueous REE signatures (2019)

Skeletal and non-skeletal components of marine sedimentary rocks have been analyzed for the purpose of reconstructing the rare earth element (REE) and yttrium (Y) compositions of paleo-seawater, but skeletal carbonates frequently have proven to be unreliable recorders of seawater chemistry. Here, we present a systematic multi-technique assessment of rare earth and other trace elements in ooid sands from the modern Great Bahama Bank (GBB–marine) and Great Salt Lake (GSL–continental) based on strong-acid (hydrofluoric and nitric) and weak-acid (acetic) digestions, as well as laser ablation (LA) of ooid cortices and nuclei. The results show that Bahamian ooid cortices possess shale-normalized REE + Y features nearly identical to those of shallow seawater, including limited contamination from siliciclastic REEs. An admixture of even 0.2% of detritally sourced material can modify the primary marine REE + Y patterns by, for example, increasing the light REE (LREE) content. Mean values of LA data for Bahamian ooid cortices exhibit similar REE + Y signatures to those produced by acetic acid digestion, but LA data are generally noisier, primarily as a result of low REE concentrations and the small volume of carbonate ablated in analysis. Screening out samples with ΣREE <0.9 ppm reveals more uniform, seawater-like REE + Y patterns in individual ooid cortices as well as high, uniform REE distribution coefficients (116 ± 21, from La to Lu) with respect to ambient seawater. Ooid laminae show no distinct alternation between oxic and anoxic pore fluid characteristics, suggesting that growing ooids primarily formed under oxic conditions in carbonate shoal settings, even when buried during their resting phase. Unlike Bahamian ooids, shale-normalized REE + Y patterns of GSL ooids digested in weak acid show slightly depleted LREE compositions that may deviate from ambient fluids owing to release of REEs from an included clay fraction based on comparison between digestion and LA methods. Multiple ablation spots within the same cortex allowed direct comparison of more and less contaminated portions of a single ooid. Nearly uniform positive Ce anomalies may be related to strongly alkaline water conditions. Individual ablation spots that are partially overprinted by siliciclastic-derived REEs contain variable Zr contents (from 0.096 to ~4 ppm) and flattened shale-normalized LREE patterns. The least-contaminated GSL ooid cortex yields an LREE-depleted REE + Y distribution. Normalization of the REE + Y distributions of least-contaminated GSL ooids using reliable GBB ooid distributions (i.e., with ΣREE > 0.9 ppm) returns a flat pattern, suggesting similar degrees of LREE depletion controlled by carbonate complexation under similar aqueous alkalinity conditions in Great Salt Lake and Bahamian waters. In summary, ooids can be a reliable proxy for REE + Y characteristics of ambient surficial waters when adopting suitable analytical methods, including laser ablation, that allow the identification and isolation of a contamination signal from siliciclastic detritus.
Gap Junctions and Connexon Hemichannels in Human Embryonic Stem Cells (2006)

Intercellular communication via gap junctions is thought to play an important role in embryonic cell survival and differentiation. Classical studies demonstrated both dye and electrical coupling of cells in the inner cell mass of mouse embryos, as well as the development of restrictions against coupling between cells of the inner cell mass and surrounding trophectoderm. Here we demonstrate extensive gap junctional communication between human embryonic stem (ES) cells, the pluripotent cells isolated from the inner cell mass of preimplantation blastocysts. Human ES cells maintained in vitro expressed RNA for 18 of the 20 known connexins; only connexin 40.1 (Cx40.1) and Cx50 were not detected by reverse transcription-polymerase chain reaction. Cx40, Cx43, and Cx45 were visualized by immunofluorescence at points of contact between adjacent cells. Electron microscopy confirmed that neighboring cells formed zones of tight membrane apposition characteristic of gap junctions. Fluorescent dye injections demonstrated extensive coupling within human ES cell colonies growing on mouse embryonic fibroblast (MEF) feeder cells, whereas dye coupling between human ES cells and adjacent MEFs was extremely rare. Physiological recordings demonstrated electrical and dye coupling between human ES cells in feeder-free monolayers and between isolated human ES cell pairs. Octanol, 18-alpha-glycyrrhetinic acid, and arylaminobenzoates inhibited transjunctional currents. Dye uptake studies on human ES cell monolayers and recordings from solitary human ES cells gave evidence for the surface expression of connexon hemichannels. Human ES cells provide a unique system for the study of human connexin proteins and their potential functions in cellular differentiation and the maintenance of pluripotency.
Novel Thyrointegrin αvβ3 Antagonist for the Treatment of Acute Myeloid Leukemia (2018)

Abstract Acute myeloid leukemia accounts for up to one-third of the more than 60,000 leukemias diagnosed annually in the U.S. Primary AML cells express membrane αvβ3 integrin, which is associated with adverse prognosis and resistance to chemotherapies used in AML. A macromolecule Polyethylene glycol-conjugated bi-TriAzole Tetraiodothyroacetic acid (P-bi-TAT) acts with high affinity (Ki 3.1 nM) and specificity for the thyrointegrin αvβ3 receptors, without nuclear translocation and has demonstrated effective suppression of cancer cell proliferation, NF-kB expression and invasion in leukemic cells. We evaluated P-bi-TAT in two different AML models against two forms of acute leukemia (monocytic and myelocytic) that are largely resistant to existing therapy, by grafting human leukemia cells in immunocompromised male and female mice. IVIS imaging scans revealed that leukemic colonies were extensively established in bone marrow throughout the control (untreated) grafted animals, as well in liver, lung and kidney. Smears of bone marrow aspirates from untreated animals were found to contain multinucleate myeloblast and monoblast leukemic cells, and peripheral blood smears contained blast cells, multinucleated megakaryocytes, giant platelets and platelet aggregates, which are hallmarks of acute leukemia. IVIS imaging scans revealed 95% reduction in bone marrow colonies and resolution of liver, kidney and lung colonies in animals treated with P-bi-TAT at daily doses ranging from 1-10 mg/kg, subcutaneously for 2-3 weeks. Peripheral blood smears from treated animals were normal. Normal myeloblasts, which are the source of functional white blood cells, were found in the marrow smears, but leukemic cells were not detected in P-bi-TAT treated animals. Thus, against two forms of leukemia models, P-bi-TAT was extraordinarily effective, with the potential in treating most AML sub-types because αvβ3 receptors are expressed in the majority of AML. Among genes targeted by multiple laboratories for pharmacological downregulation of expression in AML are BCL2, VEGF, AKT1, KIT, IDH2, CDK4/6, TIMP1, VEGF, EGFR, and PD-L1. In that regard, P-bi-TAT has been shown in various tumor cell models to downregulate transcription of each of the genes listed, which are relevant to AML disease progression. Additionally, the pro-apoptotic P53 gene transcription is enhanced by P-bi-TAT. In conclusion, P-bi-TAT is a promising lead clinical candidate that warrants clinical trials in AML patients. Disclosures Mousa: NanoPharmaceuticals LLC: Equity Ownership, Patents &amp; Royalties. Davis:NanoPharmaceuticals LLC: Employment, Equity Ownership.
Quasi-Optical Theory of Relativistic Cherenkov Oscillators and Amplifiers with Oversized Electrodynamic Structures (2022)

Using the quasi-optical approach, we investigate wave propagation along the periodically corrugated surfaces and their interaction with rectilinear relativistic electron beams (REBs). At the periodical structure, the field can be expanded into a series of spatial harmonics, which, in the case of shallow corrugations, represent paraxial wavebeams with mutual coupling described within the method of effective surface magnetic currents. We present the dispersion equation for the normal waves. Two limit cases can be recognized: in the first one, the frequency is far from the Bragg resonance and the wave propagation can be described within the impedance approximation with the field presented as a sum of the fundamental slow wave and its spatial harmonics. In the interaction with a rectilinear REB, this corresponds to the convective instability of particles’ synchronism with the fundamental (0th) or higher spatial harmonics (TWT regime), or the absolute instability in the case of synchronism with the −1st harmonic of the backward wave (BWO regime). In the latter case, at the frequencies close to the Bragg resonance, the field is presented as two antiparallel quasi-optical wavebeams, leading to the absolute instability used in the surface-wave oscillators operating in the π-mode regime. Based on the developed theory, we determine the main characteristics of relativistic Cherenkov amplifiers and oscillators with oversized electrodynamical systems. We demonstrate the prospects for the practical implementation of relativistic surface-wave devices in submillimeter wavebands.
Silicon-29 NMR evidence of alkoxy substituted aqueous silicate anions (1999)

Silicon-29 NMR provides the first direct evidence for the presence of stable alkoxy substituted silicate anions in aqueous alkaline silicate solutions to which aliphatic mono- or poly-hydroxy alcohols have been added.
RESPONSE OF GROWTH HORMONE AGGREGATES TO HIGH-INTENSITY INTERMITTENT EXERCISE (2002)

The purpose of this study was to determine the impact of high-intensity intermittent exercise on the presence of circulating growth hormone (GH) aggregates measured using two different assay techniques. Six male subjects participated in this study. After resting blood sampling, subjects completed an intermittent treadmill exercise protocol at four speeds predicted to elicit a specific VO2: 60% VO2max for 10 min, 75% for 10 min, 90% for 5 min, and 100% for 2 min. After each workload was completed treadmill speed was reduced to a walk (3.5-4 min) for blood sampling. Sampling continued every 15 minutes for one hour into recovery. All samples were then measured for GH concentrations using Nichols IRMA and DSL's immunofunctional assay (IFA). A second set of samples was chemically reduced using reduced glutathione (GSH) (10 mM for 18 hrs at room temp.) to break disulfide bonds between possible oligomeric GH complexes, and subsequently assayed using the same GH assays. With the IRMA, GH was significantly elevated (P < 0.05) after the 75% workload and remained elevated through 30 min post-exercise. After adding GSH to the sample, the IRMA indicated significant increases in GH as early as the 60% workload and remained elevated through 45 min into recovery. At 75%, the GSH assay run was significantly higher than the non-GSH assay run. With the IFA, GH was significantly elevated at 60% in the non-GSH condition, whereas the GSH assay run indicated significant elevations at 75%. Both GSH and non-GSH conditions remained elevated through 30 min into recovery. These data indicate that the addition of GSH to serum samples prior to assay via an IRMA may break existing disulfide bonds between aggregated GH molecules, thus altering the apparent assay signal to reveal greater total GH in the sample.
Tissue Culture in Ornamental Plant Breeding: A Review (2001)

Ornamental plant breeding, as other crop breeding, is considered the art and science of genetic alteration/transmutation of plants for human consumption. This paper presents a review of the literature on tissue culture in ornamental plant breeding and its applications.
Closed solutions of wave propagation in finite–periodic strings (2004)

A novel method is presented to exactly solve the progressive and standing waves for N–periodic stepwise strings. A new two–way state–flow graph model is introduced to describe the relations of waves propagating in a periodic system. Based on the model, general solutions of the wave response in the periodic string are derived by a topology scheme. Thus, the harmonic driving waves in the string with one end fixed and one end free are determined and represented in a concise closed form in terms of the properties of a single period. Analytical standing waves of the string with free–free, free–fixed and fixed–fixed constraints are also presented. Finally, examples of a three–period string with two segments in each period and a special uniform string are examined to demonstrate the present method.
Thermal conductivity measurements of DMSO and pure water ice. (2015)

&lt;p&gt;The Cahill-Pohl model for thermal conductivity of amorphous solids is calculated with Eq (&lt;a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125862#pone.0125862.e008" target="_blank"&gt;8&lt;/a&gt;) for 10 M DMSO. Based on cryomacroscope observations, DMSO concentrations of 6M or less underwent crystallization while concentrations of 7.05M and above vitrified.&lt;/p&gt;
Disruption of an M. tuberculosis Membrane Protein Causes a Magnesium-dependent Cell Division Defect and Failure to Persist in Mice (2015)

The identification of Mycobacterium tuberculosis genes necessary for persistence in vivo provides insight into bacterial biology as well as host defense strategies. We show that disruption of M. tuberculosis membrane protein PerM (Rv0955) resulted in an IFN-γ-dependent persistence defect in chronic mouse infection despite the mutant's near normal growth during acute infection. The perM mutant required increased magnesium for replication and survival; incubation in low magnesium media resulted in cell elongation and lysis. Transcriptome analysis of the perM mutant grown in reduced magnesium revealed upregulation of cell division and cell wall biosynthesis genes, and live cell imaging showed PerM accumulation at the division septa in M. smegmatis. The mutant was acutely sensitive to β-lactam antibiotics, including specific inhibitors of cell division-associated peptidoglycan transpeptidase FtsI. Together, these data implicate PerM as a novel player in mycobacterial cell division and pathogenesis, and are consistent with the hypothesis that immune activation deprives M. tuberculosis of magnesium.
Preferred conformation of peptides based on cycloaliphatic C?,?-disubstituted glycines: 1-amino-cycloundecane-1-carboxylic acid (Ac11c) (2000)

Two complete series of N-protected oligopeptide esters to the pentamer level from 1-amino-cycloundecane-1-carboxylic acid (Ac11c), an alpha-amino acid conformationally constrained through a medium-ring C(i)alpha<-->C(i)alpha cyclization, and either the L-Ala or Aib residue, along with the N-protected Ac11c monomer and homo-dimer alkylamides, have been synthesized by solution methods and fully characterized. The preferred conformation of these model peptides has been assessed in deuterochloroform solution by FT-IR absorption and 1H-NMR techniques. Furthermore, the molecular structures of one derivative (Z-Ac11c-OH) and two peptides (the tripeptide ester Z-Aib-Ac11c-Aib-OtBu and the pentapeptide ester Z-Ac11c-(Aib)2-Ac11c-Aib-OtBu) have been determined in the crystal state by X-ray diffraction. The experimental results support the view that beta-bends and 3(10)-helices are preferentially adopted by peptides rich in Ac11c, the second largest cycloaliphatic C(alpha,alpha)-disubstituted glycine known. This investigation has allowed the authors to approach the completion of a detailed conformational analysis of the whole 1-amino-cycloalkane-1-carboxylic acid (Ac(n)c, with n = 3-12) series, which represents the prerequisite for their recent proposal of the 'Ac(n)c scan' concept.
Tumor-Derived Extracellular Mutations of PTPRT/PTPρ Are Defective in Cell Adhesion (2008)

Receptor protein tyrosine phosphatase T (PTPRT/PTPrho) is frequently mutated in human cancers including colon, lung, gastric, and skin cancers. More than half of the identified tumor-derived mutations are located in the extracellular part of PTPrho. However, the functional significance of those extracellular domain mutations remains to be defined. Here we report that the extracellular domain of PTPrho mediates homophilic cell-cell aggregation. This homophilic interaction is very specific because PTPrho does not interact with its closest homologue, PTPmu, in a cell aggregation assay. We further showed that all five tumor-derived mutations located in the NH(2)-terminal MAM and immunoglobulin domains impair, to varying extents, their ability to form cell aggregates, indicating that those mutations are loss-of-function mutations. Our results suggest that PTPrho may play an important role in cell-cell adhesion and that mutational inactivation of this phosphatase could promote tumor migration and metastasis.
Visually steerable sound beam forming system based on face tracking and speaker array (2004)

This paper presents a novel human-machine interface, named invisible messenger. It integrates real time visual tracking of face and sound beam forming by speaker array. Direction towards a target person can be obtained by the face tracking on real-time. By continuously updating the sound beam direction with the face tracking output, the system can keep transmitting sounds towards the target person selectively, even if he or she moves around. Thus, it realizes remote whispering effect as if an invisible messenger were standing by him. Construction of a working system and actual measurement using the system prove the feasibility and effectiveness of the proposed idea.
Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae (2016)

While a number of allergens from house dust mites have been described, much remains to be discovered about allergens from storage mites. Here, next-generation sequencing was combined with MS/MS shotgun proteomics to identify proteins, especially potential allergens from Tyrophagus putrescentiae, commonly found in stored food products, especially flour. cDNAs of suspected allergens were cloned and expressed from bacterial cells, and recombinant allergens were tested for binding to IgE in sera from T. putrescentiae-sensitive patients. These analyses identified three previously uncharacterized allergens, Tyr p 28, Tyr p 35, and Tyr p 36, which have been officially assigned by the WHO/IUIS Allergen Nomenclature Sub-committee. Recombinant proteins rTyr p 28, rTyr p 35, and rTyr p 36 bound with 47.1%, 82.4%, and 70.6% of T. putrescentiae-sensitive sera. We provide here a new method to identify allergens by the combination of transcriptomic and proteomic approaches.
Transfer of Cardiac Mitochondria Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in a Preclinical Model of Ischemic Heart Disease (2023)

The preconditioning of MSCs with the artificial transfer of cardiac mitochondria appears to be promising strategy to improve the efficacy of MSC-based cell therapy in ischemic heart disease.
Collaborative fingerprint authentication by smart card and a trusted host (2002)

The smart card is an important component in e-commerce security. In a previous CCECE paper, we introduced the idea for verification of the ownership of a smart card using fingerprints. An owner's fingerprint is registered into a smart card. When using a smart card on a computer, the card software will match the user's fingerprint with that stored in the card. This paper describes a continuation of this research. Our goal is to extend the role of the smart card to become an active authenticator for participation in the fingerprint authentication process. The heart of the problem lies in the limited computing power of the card's processor. This paper reports the detailed descriptions of the design, implementation and experiments.
Fractalkine suppression during hepatic encephalopathy promotes neuroinflammation in mice (2016)

These findings suggest that fractalkine-mediated signaling is suppressed in the brain following the development of hepatic encephalopathy. Supplementation of AOM-treated mice with soluble fractalkine led to improved outcomes, which identifies this pathway as a possible therapeutic target for the management of hepatic encephalopathy following acute liver injury.
Taking heart failure seriously (2000)

General practice p 1113 

Heart failure is common, serious, and treatable, 1 2 so great efforts should be made to manage it correctly. In this issue Mason et al point out that fear of side effects may be a major barrier to the use of angiotensin converting enzyme inhibitors by general practitioners (p 1113).3 However, management is not just about prescribing the correct treatment; it also involves obtaining a proper diagnosis.

An estimated 10 million patients in Europe have heart failure secondary to left ventricular systolic dysfunction. 1 2 They are dwarfed by the estimated additional 40 million who have symptoms and signs of heart failure2 but in whom the diagnosis is subsequently refuted by investigation4 or attributed to another cause such as “diastolic” heart failure,2 a condition that is not known to respond to angiotensin converting enzyme inhibitors.5 Only once a diagnosis of heart failure has been established and underlying left ventricular systolic dysfunction confirmed is treatment with a combination of angiotensin converting enzyme inhibitors and β blockers (Cochrane reviews in preparation) generally appropriate.

Who then should manage patients with suspected or confirmed heart failure? The diagnostic burden of suspected heart failure is about 50 000 patients per million population, of which …
Supplementary Figures 1-4 from The Metabolomic Signature of Malignant Glioma Reflects Accelerated Anabolic Metabolism (2023)

&lt;p&gt;PDF file - 237K, Figure S1. RF analysis was performed to determine the accuracy of metabolomic profiles in predicting glioma grade. Figure S2. Intra-sample concordance of glycolytic intermediates PEP and 3-PG in glioma. Figure S3. Glioma subtype classification based on transcriptional profiles was performed using unsupervised hierarchical clustering from a total of 35 probe sets, as described as Phillips et al. (15). Figure S4. PCA of each metabolic profile by plotting the first two components, indicating no discernable separation between transcriptional subtypes or grade.&lt;/p&gt;