gebhart/patrepeval huggingface dataset:
Inactivation of rat renal phosphate-dependent glutaminase with 6-diazo-5-oxo-L-norleucine. Evidence for interaction at the glutamine binding site. (1979)

Inactivation of rat renal phosphate-dependent glutaminase by 6-diazo-5-oxo-L-norleucine occurs only under conditions where the enzyme is catalytically active. The glutaminase activity and the rate of inactivation by the diazoketone exhibit very similar phosphate concentration-dependent activation profiles. Because of this phosphate dependency, it was not possible to differentiate an apparent protection by glutamine from the strong inhibition of inactivation caused by glutamate. The ability of glutamate to protect the glutaminase against inactivation is reversed by increasing concentrations of phosphate. The observed characteristics of inactivation by 6-diazo-5-oxo-L-norleucine differ considerably from those reported for the inactivation by L-2-amino-4-oxo-5-chloropentanoic acid. In addition, the presence of o-carbamoyl-L-serine was found to stimulate inactivation by 6-diazo-5-oxo-L-norleucine, but to protect the glutaminase against inactivation by the chloroketone. Preinactivation of the glutaminase by the diazoketone only slightly reduced the stoichiometry of binding of [5-14C]chloroketone. These observations suggest that 6-diazo-5-oxo-L-norleucine and L-2-amino-4-oxo-5-chloropentanoic acid interact with different sites on the glutaminase which are specific for binding glutamine and glutamate, respectively.
Neurite Development in PC12 Cells on Nanostructured Substrates (2006)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Insulin increases branched-chain α-ketoacid dehydrogenase kinase expression in Clone 9 rat cells (2002)

The branched-chain amino acids (BCAA) are committed to catabolism by the activity of the branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. BCKD activity is regulated through the action of the complex-specific BCKD kinase that phosphorylates two serine residues in the E1alpha subunit. Greater BCKD kinase expression levels result in a lower activity state of BCKD and thus a decreased rate of BCAA catabolism. Activity state varies among tissues and can be altered by diet, exercise, hormones, and disease state. Within individual tissues, the concentration of BCKD kinase reflects the activity state of the BCKD complex. Here we investigated the effects of insulin, an important regulator of hepatic metabolic enzymes, on BCKD kinase expression in Clone 9 rat cells. Insulin effected a twofold increase in message levels and a twofold increase in BCKD kinase protein levels. The response was completely blocked by treatment with LY-294002 and partially blocked by rapamycin, thus demonstrating a dependence on phosphatidylinositol 3-kinase and mTOR function, respectively. These studies suggest that insulin acts to regulate BCAA catabolism through stimulation of BCKD kinase expression.
Antibody response toStaphylococcus aureuswhole cell, lipase and staphylolysin in patients withS. aureusinfections (1992)

Three assays to measure antibodies against Staphylococcus aureus whole cells, lipase and staphylolysin were used to try to discriminate between complicated and uncomplicated S. aureus septicaemia. Sera were examined from 8 patients with S. aureus endocarditis, 23 patients with complicated S. aureus septicaemia, 12 patients with uncomplicated S. aureus septicaemia and 93 febrile non-septicaemic controls. No single assay could distinguish between complicated and uncomplicated S. aureus septicaemia. If the criterion for a positive result is defined as positive antibody level in the anti-lipase ELISA as well as in at least 1 of the other 2 assays, 10/31 patients with S. aureus endocarditis or complicated septicaemia were positive compared to 0/93 non-septicaemic patients and 0/12 patients with uncomplicated S. aureus septicaemia. Therefore, the combined use of serological assays in the diagnosis of complicated S. aureus septicaemia, one of which is the anti-lipase ELISA, is recommended.
Three-dimensional structure formed by holographic two-photon microfabrication of photoresist (2007)

Holographic two-photon microfabrication method performs high-speed parallel processing inside photosensitive materials using a computer-generated hologram displayed on a liquid crystal spatial light modulator. We demonstrate three-dimensional microfabrication using photoresist.
A perspective on quantifying resilience: Combining community and infrastructure capitals (2022)

The resilience of communities has emerged as a major goal in policy and practice. Cities, states, and counties within the United States and around the world are passing laws requiring the incorporation of climate-related hazard vulnerability assessments within their master plan updates for resilience planning and design. The resilience of communities under present and future scenarios is thus becoming a cornerstone of decision making and actions. Decisions that would enhance resilience, however, span multiple sectors and involve various stakeholders. Quantifying community resilience is a key step in order to describe the preparedness level of communities, and subsequently locating non-resilient areas to further enhance their capacity to endure disasters. Two main approaches are currently being pursued to evaluate resilience. The first approach is the "community resilience" developed mainly by social scientists and planners, and it captures social resilience using numerous pre-disaster attributes to describe the functioning of a community. This approach subsumes that pre-disaster attributes can predict the community resilience to a disaster. The second approach is adopted for infrastructure resilience, mostly used by engineers, and it focuses on robustness, redundancy, resourcefulness, and rapidity. This approach is appropriate for systems that are operated by highly skilled personnel and where the actions are of engineering type. In this paper, we provide an overview of the two approaches, and we leverage their limitations to propose a hybrid approach that combines community and infrastructure capitals into an Area Resilience metric, called ARez. ARez captures the role/impact of both infrastructure and community and combines five sectors: energy, public health, natural ecosystem, socio-economic, and transportation. We present a proof-of-concept for the ARez metric, showing its practicality and applicability as a direct measure for resilience, over various time scales.
Magnetic suspension and balance system (MSBS) advanced study.I - System design (1987)

A magnetic suspension and balance system is designed to support models of aircraft or other objects in wind tunnels by means of magnetic forces. Major design improvements have been achieved, resulting in reductions of the system size, weight, and cost. These improvements are due to: (1) the use of holmium in the model core to increase its magnetic moment, (2) the use of a powerful new permanent magnet material in the model wings, (3) a new arrangement for the roll coils, and (4) the use of a nonmetallic structure to eliminate eddy current losses. The conceptual design of the holmium core superconductive solenoid and of the new permanent magnet wing assembly is described in detail. The discussion includes comparisons of the pole strengths for different model core magnets, the design of a superconducting solenoid and cryostat, and the analysis of model wing magnetic requirements.
Protein tyrosine kinases in activation signal of human basophils through the immunoglobulin E receptor type I (1996)

Human basophils activated through high-affinity immunoglobulin E (IgE) receptors (Fc epsilon RI) are involved in the late phase of the allergic reaction. To investigate the possible involvement of protein-tyrosine kinases in this activation we used human acute basophilic leukemia (ABL) cells in culture as well as a pure population of normal basophils in vitro-derived from human bone marrow precursor cells (HBMB). ABL cells were 50-80% basophils at various stages of maturation as assessed by staining, morphology, ultrastructure, and flow cytometry analysis, and only basophils in ABL cells expressed Fc epsilon RI. Aggregation of Fc epsilon RI by IgE and anti-IgE, IgE and antigen, or anti-Fc epsilon RI monoclonal antibodies on ABL cells or on HBMB, led to increased tyrosine phosphorylation of 120-, 100-, 80-, 72-, 50- to 65-, and 38-kDa substrates. Tyrosine phosphorylations in ABL cells were in basophils because 1) they were detected after a 5-s stimulation, 2) they were observed under conditions where mediator release is minimal, i.e., in the absence of extracellular calcium, 3) hapten addition during antigen stimulation resulted in almost total disappearance of tyrosine phosphorylations within 30 s. There was correlation between histamine release and tyrosine phosphorylation in anti-IgE dose-responses and in dose-responses of the tyrosine kinase inhibitor genistein. The tyrosine kinase p72syk was detected in the cells. Stimulation of ABL cells for 1 min resulted in extracellular calcium-independent tyrosine phosphorylation and activation of p72syk. Therefore, tyrosine kinases are involved in the early steps of human Fc epsilon RI signaling in basophils. Tyrosine kinases and their substrates could represent new potential therapeutic targets to prevent the development of the allergic reaction.
High surgical volume is associated with a lower rate of secondary therapy after radical prostatectomy for localized prostate cancer (2008)

The use of ST is inversely proportional to SV of up to 24 RPs per year. A higher annual SV might be indicative of less restrictive use of RP in high-risk patients who eventually require combined treatments.
Crystal Chemistry and Dehydrogenation/Rehydrogenation Properties of Perovskite Hydrides RbMgH3 and RbCaH3 (2009)

Crystal structure, lattice dynamics, and bonding environments of RbMgH3 and RbCaH3 were investigated using neutron powder diffraction (NPD), neutron vibrational spectroscopy (NVS), and first-principles calculations. RbMgH3 exhibits a 6H-BaTiO3-type hexagonal perovskite structure, and RbCaH3 forms a Pm-3m simple cubic perovskite. A very short Mg−Mg distance (∼2.77 Å) was found in RbMgH3, and its structural stability was ascribed to H− anion polarization and Mg−Mg bonding. Interestingly, RbCaH3 forms an ideal cubic perovskite despite its small tolerance factor. The inconsistency between the nominal tolerance factor and adopted structure types of RbCaH3 are discussed in terms of anion polarization and covalence effect on the cation−anion bond length deviations. Finally, we show that both RbMgH3 and RbCaH3 can be dehydrogenated and rehydrogenated at 300−400 °C under moderate pressures.
High Assembly Tolerance and Cost-Effective 100-Gb/s TOSA With Silica-PLC AWG Multiplexer (2019)

A low-cost hybrid-integrated transmitter optical sub-assembly (TOSA) for a 100-Gb/s coarse wavelength division multiplexing (CWDM) transceiver with high assembly tolerance has been demonstrated. We have proposed a novel two-lens configuration for the coupling between distributed feedback laser diodes and a silica-based planar lightwave circuit arrayed waveguide grating to relax the lenses assembly requirement. For this TOSA, the 1-dB assembly tolerances of all lenses are over ±15 μm. Furthermore, we use a flexible printed circuit to connect the interior and exterior of the TOSA for radiofrequency signal transmission instead of the conventional ceramic feed-through box. Finally, a TOSA was packaged with a size of 22 mm × 10.7 mm × 4.5 mm. Error-free transmission over a 10-km single-mode fiber was successfully achieved when all four lanes were driven simultaneously with a total bit rate of 4 × 25.78125 Gb/s at an operating temperature of 25 °C.
Anti-inflammatory activities of Aller-7, a novel polyherbal formulation for allergic rhinitis. (2004)

Allergic rhinitis is an immunological disorder and an inflammatory response of nasal mucosal membranes. Allergic rhinitis, a state of hypersensitivity, occurs when the body overreacts to a substance such as pollens or dust. A novel, safe polyherbal formulation (Aller-7/NR-A2) has been developed for the treatment of allergic rhinitis using a unique combination of extracts from seven medicinal plants including Phyllanthus emblica, Terminalia chebula, Terminalia bellerica, Albizia lebbeck, Piper nigrum, Zingiber officinale and Piper longum. Since inflammation is an integral mechanistic component of allergy, the present study aimed to determine the anti-inflammatory activity of Aller-7 in various in vivo models. The efficacy of Aller-7 was investigated in compound 48/80-induced paw edema both in Balb/c mice and Swiss Albino mice, carrageenan-induced paw edema in Wistar Albino rats and Freund's adjuvant-induced arthritis in Wistar Albino rats. The trypsin inhibitory activity of Aller-7 was also determined and compared with ovomucoid. At a dose of 250 mg/kg, Aller-7 demonstrated 62.55% inhibition against compound 48/80-induced paw edema in Balb/c mice, while under the same conditions prednisolone at an oral dose of 14 mg/kg exhibited 44.7% inhibition. Aller-7 significantly inhibited compound 48/80-induced paw edema at all three doses of 175, 225 or 275 mg/kg in Swiss Albino mice, while the most potent effect was observed at 225 mg/kg. Aller-7 (120 mg/kg, p.o.) demonstrated 31.3% inhibition against carrageenan-induced acute inflammation in Wistar Albino rats, while ibuprofen (50 mg/kg, p.o.) exerted 68.1% inhibition. Aller-7 also exhibited a dose-dependent (150-350 mg/kg) anti-inflammatory effect against Freund's adjuvant-induced arthritis in Wistar Albino rats and an approximately 63% inhibitory effect was observed at a dose of 350 mg/kg. The trypsin inhibitory activity of Aller-7 was determined, using ovomucoid as a positive control. Ovomucoid and Aller-7 demonstrated IC50 concentrations at 1.5 and 9.0 microg/ml, respectively. These results demonstrate that this novel polyherbal formulation is a potent anti-inflammatory agent that can ameliorate the symptoms of allergic rhinitis.
The surgical treatment of exertional compartment syndrome in athletes. (1983)

We studied the cases of twelve patients who had bilateral exertional compartment syndrome involving one or all of the compartments of the lower extremities. The patients were divided into three groups based on the clinical findings. Group I consisted of seven patients with symptoms involving the anterior or lateral compartments only; Group II, of three patients with involvement of the deep posterior compartments alone; and Group III, of two patients with involvement of both the anterior and deep posterior compartments. On the basis of their clinical signs and symptoms each patient underwent dynamic tissue-pressure measurements using a slit catheter before, during, and for as long as thirty minutes after exercise. All patients had elevated resting and peak-to-peak dynamic pressure measurements. Treatment consisted of bilateral compartmental fasciotomy, as indicated. All of the patients in Group I had complete relief of symptoms; however, one patient in Group II and one in Group III had a recurrence of symptoms in the deep posterior compartment.
Validation of a T1and T2* leakage correction method based on multiecho dynamic susceptibility contrast MRI using MION as a reference standard (2015)

Correcting for both T1 and T2* leakage effects yielded more accurate measures of CBV. The combined correction method yields more reliable CBV measures than either correction method alone, but for certain brain tumor types (e.g., gliomas), the simplified biophysical method may provide a robust and computationally efficient alternative. Magn Reson Med 76:613-625, 2016. © 2015 Wiley Periodicals, Inc.
Haploscleridamine, a Novel Tryptamine-Derived Alkaloid from a Sponge of the Order Haplosclerida:  An Inhibitor of Cathepsin K (2002)

As part of a search for novel inhibitors of cathepsin K, the MeOH extract of a Micronesian sponge of the order Haplosclerida was shown to be active. Bioassay-guided fractionation of the extract yielded halitoxins, tryptamine, and a novel tryptamine-derived alkaloid, haploscleridamine (1). The tetrahydro-beta-carboline structure of haploscleridamine (1) was elucidated through spectral techniques. Haploscleridamine (1) was found to be an inhibitor of cathepsin K with an IC(50) of 26 microM.
Universal Screening for Mismatch-Repair Deficiency in Endometrial Cancers to Identify Patients With Lynch Syndrome and Lynch-like Syndrome (2016)

Although consensus has yet to be reached on universal mismatch-repair (MMR) protein immunohistochemical (IHC) screening for Lynch syndrome (LS) in endometrial cancer (EC), an increasing number of institutions have adopted universal screening protocols similar to those used for colorectal carcinoma. Here we describe our institution's experience with a prospective universal screening protocol in which all ECs resected over a period of 19 months (n=242) were screened for MLH1, PMS2, MSH2, and MSH6 deficiencies using IHC, followed by MLH1 promoter methylation testing when appropriate. When consent was obtained, tumor samples underwent next-generation sequencing. A total of 11 unmethylated MMR-deficient cases (4.5% of cohort) were identified through IHC screening. Germline testing was performed in 10 cases and confirmed LS in 4 patients (1.7% of cohort). Of our 4 confirmed LS cases, 1 did not meet traditional LS screening criteria (eg, age below 50 y, Revised Bethesda criteria). In addition, universal screening identified 6 germline-negative MMR-deficient nonmethylated cases, 4 of which occurred in women older than 50. Although our next-generation sequencing data suggest somatic mutations in 4 of these cases, it is possible that these cases may represent cases of "Lynch-like syndrome." We conclude that a subset of LS cases could be missed using traditional screening guidelines. The value of screening for Lynch-like syndrome has yet to be determined. Although the cost-effectiveness of universal screening in EC has yet to be elucidated, we conclude that universal IHC screening is currently a reasonable, and arguably superior, approach to screening for LS.
Characterisation of lymphocyte migration following DNA vaccination. (2005)

Infection with influenza virus generally produces an immune response in immunocompetent individuals that will protect from further infection of the same strain of virus. The experiments carried out in this study were designed to identify correlates of protection induced by primary experimental influenza virus infection and to compare to/and assess Particle Mediated Immunotherapeutic Delivery (PMID) as a method of DNA immunisation. Initial experiments characterised the protective immune response observed following intranasal influenza virus infection with regard to the humoral and cellular responses generated. It became clear that a combination of systemic and mucosal antibody responses and strong virus-specific CD8+ T cell response in the lungs were important in the control of viral replication. The data on the immune responses generated following influenza virus infection was used as a "gold standard" to compare the responses observed following PMID immunisation of influenza virus nucleoprotein (NP) DNA. When PMID was assessed for its ability to generate an immune response, a mucosal immune response was observed in the D-NALT as well as a more general systemic response. Whilst a single immunisation induced specific cellular responses, it proved inefficient at generating a humoral response. Protection studies showed that priming by PMID of NP DNA, resulted in a level of protection that reduced viral replication in the lungs by 2 logs. Investigation into the phenotype of virus-specific T cells generated by either infection or immunisation showed that they have a common CD44hiCD11ahlCD62L10 phenotype in various systemic and mucosal tissues. The cells isolated from the D-NALT displayed an intriguing intermediate level of CD44 expression. In vivo migration studies showed a tendency for activated splenic virus-specific CD8+ T cells to home to the D-NALT perhaps implying a role for the D-NALT as a site where effector/memory T cells actively home as part of their role in immune surveillance.
Marketing computational intelligence in industry (2010)

The paper gives guidelines for a systematic marketing approach for promoting computational intelligence in industry. The proposed research marketing strategy includes three key steps. The first step identifies the target market (industry, government, or academia). The second step defines and protects the product. The third step implements the promotional strategy by publishing, conference presentations, and technical and business advertising. Different mechanisms of marketing computational intelligence through interactions between academia and industry, such as protecting intellectual property, publishing, conference advertising, technology development, and interactions with vendors are discussed. Specific directions for targeting technical and nontechnical audience are given as well.
Crosslinked Capsules of Quantum Dots by Interfacial Assembly and Ligand Crosslinking (2005)

Robust crosslinked capsules are produced from water droplets stabilized by oil/water interfacial assembly of norbornene-functionalized CdSe/ZnS core–shell quantum dots that can be crosslinked by ring-opening metathesis polymerization (see Figure). The mechanical integrity of the capsules is maintained after removal of the interface. An amphiphilic polyethylene glycol-substituted ruthenium benzylidene catalyst proved ideal for this interfacial polymerization.