gebhart/patrepeval huggingface dataset:
Supervised machine learning and associated algorithms: applications in orthopedic surgery (2022)

Supervised learning is the most common form of machine learning utilized in medical research. It is used to predict outcomes of interest or classify positive and/or negative cases with a known ground truth. Supervised learning describes a spectrum of techniques, ranging from traditional regression modeling to more complex tree boosting, which are becoming increasingly prevalent as the focus on "big data" develops. While these tools are becoming increasingly popular and powerful, there is a paucity of literature available that describe the strengths and limitations of these different modeling techniques. Typically, there is no formal training for health care professionals in the use of machine learning models. As machine learning applications throughout medicine increase, it is important that physicians and other health care professionals better understand the processes underlying application of these techniques. The purpose of this study is to provide an overview of commonly used supervised learning techniques with recent case examples within the orthopedic literature. An additional goal is to address disparities in the understanding of these methods to improve communication within and between research teams.
Analytical study of license-assisted access in 5G networks (2019)

The volume of traffic transmitted over cellular networks is growing significantly every year. To improve LTE network throughput, 3GPP has proposed License-Assisted Access (LTE-LAA), which allows utilizing the unlicensed 5GHz spectrum already used by Wi-Fi and other technologies. To enable fair resource sharing, LTE-LAA adopts CSMA/CA-based channel access with binary exponential backoff similar to Wi-Fi. However, because of the LTE-LAA technology constraints, the LTE-LAA base station may start data transmission only at rather rare licensed carrier slot boundaries. The essential question, what the base station should do between the end of the backoff procedure and the closest licensed slot boundary, is left implementation dependent. In the paper, we have developed analytical models to study the performance of different LTE-LAA implementations and analyze channel sharing fairness between LTE-LAA and Wi-Fi networks in the coexistence scenario. Moreover, we investigate how the performance of LTE-LAA channel access can be improved using New Radio flexible numerology, which is one of the main features of currently developed 5G systems.
Optimization of the radiation dosimetry protocol in Lutetium-177-PSMA therapy: toward clinical implementation (2023)

Accurate lesion dosimetry in [<sup>177</sup>Lu]Lu-PSMA therapy can be performed using a one- or two-time point protocol, making dosimetry assessments more suitable for routine clinical implementation, although dosimetry based om multiple time points is more accurate. Clinical trial registration This study was approved by the Medical Review Ethics Committee Region Arnhem-Nijmegen on January 23, 2018 and was registered on clinicaltrials.gov (NCT03828838).
A New Oxidizing System for Aromatic Alcohols by the Combination of N-Oxoammonium Salt and Electrosynthesized Tetraalkylammonium Tribromide (1991)

Abstract A combination of 2,2,6,6-tetramethyl-1-piperidinyloxyl and tetraalkylammonium tribromides (R4NBr3), which are available from the corresponding tetraalkylammonium bromides via electrooxidation with potassium bromide, has proved to be useful for oxidations of primary and secondary alcohols to the corresponding aldehydes and ketones, respectively. The oxidation reaction proceeds smoothly even with a 0.5–1.0 mol% of N-oxyl compounds and 1.5–2.0 equivalents of tetraalkylammonium tribromides in an aqueous-organic two-phase solution buffered at pH 8.0–8.6. This recyclable oxidant/co-oxidant combination system may involve the formation of N-oxoammonium salts, actual oxidizing agents of alcohols, by the action of hypobromite species generated from R4NBr3 in the binary solution. Utility of the method is highlighted by the selective oxidation of benzylic alcohols bearing electron-releasing groups on the aromatic nucleus to the corresponding aldehydes or ketones without any bromination and overoxidation.
Limited cleavage of cellular fibronectin by plasminogen activator purified from transformed cells. (1987)

The substrate specificity and direct catalytic activity of plasminogen activator (PA) was examined under conditions where its natural substrate, plasminogen, was missing or inhibited. PA, purified from cultures of transformed chicken fibroblasts, was incubated with purified preparations of potential substrates. The adhesive glycoprotein fibronectin, isolated from normal chicken fibroblast extracellular matrix, underwent limited but specific cleavage by PA in the absence of plasminogen. Analysis of the cleavage products by polyacrylamide gels under both reducing and nonreducing conditions indicated that PA-mediated cleavage occurred near the carboxyl terminus of fibronectin but on the amino-terminal side of the interchain disulfide bridge, thus disrupting the native dimeric fibronectin molecule. Under the identical conditions, chicken ovalbumin was not cleaved while the established substrate, chicken plasminogen, was extensively converted to plasmin. A monoclonal antibody, directed against avian PA and shown to inhibit plasminogen-free, cell-mediated matrix degradation, specifically inhibited the fibronectin cleavage. A human PA, urokinase, also cleaved fibronectin under plasminogen-free conditions yielding a limited number of high molecular weight cleavage products.
Genome-wide Mapping of PiggyBac Transposon Integrations in Primary Human T Cells (2009)

The piggyBac transposon system represents a promising nonviral tool for gene delivery and discovery, and may also be of value for clinical gene therapy. PiggyBac is a highly efficient integrating vector that stably transfects (approximately 40%) of primary human T cells for potential adoptive immunotherapy applications. To evaluate the potential genotoxicity of piggyBac, we compared 228 integration sites in primary human T cells to integrations in 2 other human-derived cell lines (HEK293 and HeLa) and randomly simulated integrations into the human genome. Our results revealed distinct differences between cell types. PiggyBac had a nonrandom integration profile and a preference for transcriptional units (approximately 50% into RefSeq genes in all cell types), CpG islands (18% in T cells and 8% in other human cells), and transcriptional start sites (<5 kb, 16% to 20% in all cell types). PiggyBac also preferred TTAA but not AT-rich regions of the human genome. We evaluated the expression of mapped genes into which piggyBac integrated, and found selection of more active genes in primary human T cells compared with other human cell types, possibly due to concomitant T-cell activation during transposition. Importantly, we found that in comparison to what has been reported for gammaretroviral and human lenitviral vectors, piggyBac had decreased integration frequency into or within 50 kb of the transcriptional start sites of known proto-oncogenes. Hence the piggyBac nonviral gene delivery system seems to represent a promising gene transfer system for clinical applications using human T lymphocytes.
Butyl rubber O-ring seals: Revision of test procedures for stockpile materials (1996)

Extensive testing showed little correlation between test slab and O-ring performance. New procedures, comparable to those used with the traditional test slabs, were defined for hardness, compression set, and tensile property testing on sacrificial O-ring specimens. Changes in target performance values were made as needed and were, in one case, tightened to reflect the O-ring performance data. An additional study was carried out on O-ring and slab performance vs cure cycle and showed little sensitivity of material performance to large changes in curing time. Aging and spectra of certain materials indicated that two sets of test slabs from current vendor were accidently made from EPDM rather than butyl rubber. Random testing found no O-rings made from EPDM. As a result, and additional spectroscope test will be added to the product acceptance procedures to verify the type of rubber compound used.
Interfacial properties of kevlar-49 fiber-reinforced thermoplastics (1976)

A single-filament pull-out test was used to study adhesion of Kevlar-49 fibers to thermoplastic polymers. The test involved pulling a partially embedded fiber out of a polymer film. Kevlar-49 fibers with three different surface treatments were used with five thermoplastic materials. The test resulted in the measurement of two properties, an interfacial bond strength and a frictional shear strength. The interfacial bond strength is an essential factor in determining the critical aspect ratio of discontinuous fibers in a composite. The frictional shear strength was found to correlate with the tensile strength of discontinuous fiber composites which fail by fiber pull-out. Scanning electron microscopy was used to examine the fiber pull-out specimens after testing. Observations of the fiber showed that the failure mode at the fiber–matrix interface was complex. The predominant failure mode was fracture at the interface (or in some weak boundary layer). In some cases, cohesive failure of the fiber surface was observed, with the result that strips of material were torn from the fiber surface.
Analytical and Numerical Results for an Adhesively Bonded Joint Subjected to Pure Bending (2006)

A one-dimensional, semi-analytical methodology that was previously developed for evaluating adhesively bonded joints composed of anisotropic adherends and adhesives that exhibit inelastic material behavior is further verified in the present paper.A summary of the first-order differential equations and applied joint loading used to determine the adhesive response from the methodology are also presented.The method was previously verified against a variety of single-lap joint configurations from the literature that subjected the joints to cases of axial tension and pure bending.Using the same joint configuration and applied bending load presented in a study by Yang, the finite element analysis software ABAQUS was used to further verify the semi-analytical method.Linear static ABAQUS results are presented for two models, one with a coarse and one with a fine element meshing, that were used to verify convergence of the finite element analyses.Close agreement between the finite element results and the semi-analytical methodology were determined for both the shear and normal stress responses of the adhesive bondline.Thus, the semi-analytical methodology was successfully verified using the ABAQUS finite element software and a single-lap joint configuration subjected to pure bending.
Development of a self-inactivating tet-on retroviral vector expressing bone morphogenetic protein 4 to achieve regulated bone formation (2004)

The aims of this study were to explore the possibility of improving the design of self-inactivating (SI) retroviral vectors and to develop an SI vector that would allow optimal tet-on-regulated therapeutic gene expression. To minimize any interference between the viral promoter and the inducible promoter, we deleted different regulatory elements in the 3'LTR and examined their effects on transgene expression in transfected or transduced cells. In transfected cells, such deletions reduced the transgene expression. The insertion of a polyadenylation sequence could not completely compensate for this effect. We observed three patterns of transgene expression in cells transduced with these tet-on retroviral vectors: (1) high levels of both basal and inducible expression, (2) low levels of both basal and inducible expression, and (3) low levels of basal and high levels of inducible expression. After using the optimal vector to transduce muscle-derived stem cells, we were able to regulate the strong in vitro expression of transgenes-including enhanced green fluorescent protein and bone morphogenetic protein 4-via the addition or withdrawal of doxycycline (Dox). Implantation of the transduced cells and subsequent Dox-dependent induction of gene expression resulted in bone formation in vivo. Thus, we have developed an optimal SI retroviral vector that maintains a high titer, efficiently transduces muscle-derived stem cells, and enables both high levels of inducible gene expression in vitro and robust regulated bone formation in vivo.
Measurement of Brown Adipose Tissue Activity Using Microwave Radiometry and 18F-FDG PET/CT (2018)

The aim of this study was to evaluate the operating characteristics of a microwave radiometry system in the noninvasive assessment of activated and nonactivated brown adipose tissue (BAT) and normal-tissue temperatures, reflecting metabolic activity in healthy human subjects. The radiometry data were compared with <sup>18</sup>F-FDG PET/CT images in the same subjects. <b>Methods:</b> Microwave radiometry and <sup>18</sup>F-FDG PET/CT were sequentially performed on 19 participants who underwent a cold intervention to maximize BAT activation. The cold intervention involved the participants' intermittently placing their feet on an ice block while sitting in a cool room. Participants exhibiting BAT activity qualitatively on PET/CT were scanned again with both modalities after undergoing a BAT minimization protocol (exposure to a warm room and a 20-mg dose of propranolol). Radiometry was performed every 5 min for 2 h before PET/CT imaging during both the warm and the cold interventions. A grid of 15-20 points was drawn on the participant's upper body (data were collected at each point), and a photograph was taken for comparison with PET/CT images. <b>Results:</b> PET/CT identified increased signal consistent with BAT activity in 11 of 19 participants. In 10 of 11 participants with active BAT, radiometry measurements collected during the cold study were modestly, but significantly, higher on points located over areas of active BAT on PET/CT than on points not exhibiting BAT activity (<i>P</i> < 0.01). This difference lessened during the warm studies: 7 of 11 participants showed radiometry measurements that did not differ significantly between the same set of points. The mean radiometry result collected during BAT maximization was 33.2°C ± 1.5°C at points designated as active and 32.7°C ± 1.3°C at points designated as inactive (<i>P</i> < 0.01). <b>Conclusion:</b> Passive microwave radiometry was shown to be feasible and, with substantial improvements, has the potential to noninvasively detect active brown adipose tissue without a radiotracer injection.
Yellow Light–Emitting Poly(phenylenevinylene) Incorporated with Pendant Ruthenium Bipyridine and Terpyridine Complexes (1999)

Simultaneous emission from PPV and ruthenium (II) complexes is demonstrated in the title materials (see Fig). The PPV backbone emits green light and the ruthenium polypyridine complexes emit red light resulting in overall yellow emission. At low metal complex content the hexamethylene spacer of the pendant ruthenium complexes prevents quenching of the backbone emission but at higher loading quenching becomes dominant, allowing the emission color of the materials to be tuned by adjusting the metal complex content.
A Genetic Screen For DNA Double-Strand Break Repair Mutations in Drosophila (2007)

The study of DNA double-strand break (DSB) repair has been greatly facilitated by the use of rare-cutting endonucleases, which induce a break precisely at their cut sites that can be strategically placed in the genome. We previously established such a system in Drosophila and showed that the yeast I-SceI enzyme cuts efficiently in Drosophila cells and those breaks are effectively repaired by conserved mechanisms. In this study, we determined the genetic requirements for the repair of this I-SceI-induced DSB in the germline. We show that Drosophila Rad51 and Rad54 are both required for homologous repair by gene conversion, but are dispensable for single-strand annealing repair. We provided evidence suggesting that Rad51 is more stringently required than Rad54 for intersister gene conversion. We uncovered a significant role of DNA ligase IV in nonhomologous end joining. We conducted a screen for candidate mutations affecting DSB repair and discovered novel mutations in genes that include mutagen sensitive 206, single-strand annealing reducer, and others. In addition, we demonstrated an intricate balance among different repair pathways in which the cell differentially utilizes repair mechanisms in response to both changes in the genomic environment surrounding the break and deficiencies in one or the other repair pathways.
Method of contents cache control in a BML/HTML integrated browser (2004)

We have developed a new method of contents cache control to improve performance of response to user operations in a BML/HTML integrated browser on Digital TV. By using our method, our browser can use as much memory as possible for contents cache without the lack of memory when other applications start, and pre-fetch the data which viewers might watch. We have shown that our method is effective from our experiments. Keywords-browser;digital TV; data carousel; DSM-CC; cache; BML; data broadcasting;
Type inference and type checking for queries on execution traces (2008)

This paper studies, for the first time, the management of type information for an important class of semi-structured data: nested DAGs (Directed Acyclic Graphs) that describe execution traces of business processes (BPs for short). Specifically, we consider here type inference and type checking for queries over BP execution traces. The queries that we consider select portions of the traces that are of interest to the user; the types describe the possible shape of the execution traces in the input/output of the query. We formally define and characterize here three common classes of BP execution traces and their respective notions of type inference and type checking. We study the complexity of the two problems for query languages of varying expressive power and present efficient type inference/checking algorithms whenever possible. Our analysis offers a nearly complete picture of which combinations of trace classes and query features lead to PTIME algorithms and which to NP-complete or undecidable problems.
Tannin-Rich Carob Pod for the Treatment of Acute-Onset Diarrhea (1989)

Infants aged 3-21 months with acute diarrhea of bacterial and viral origin were treated as inpatients with oral rehydration fluid and randomly received for up to 6 days either a tannin-rich carob pod powder (40% tannins or 21.2% polyphenols and 26.4% dietary fiber), 1.5 g/kg/day (n = 21) to a maximum of 15 g, or an equivalent placebo (n = 20). The duration of the diarrhea from admission was 2.0 +/- 0.27 days in the test group and 3.75 +/- 0.30 days in the placebo group (p less than 0.001). Normalized defecation, body temperature, and weight and cessation of vomiting were reached more quickly by the patients who received the test substance. The test substance was well accepted and tolerated.
Correction: Schütt et al. Simulating the Hydrodynamic Conditions of the Human Ascending Colon: A Digital Twin of the Dynamic Colon Model. Pharmaceutics 2022, 14, 184 (2022)

In the original publication [...].
Middleware support for non-repudiable business-to-business interactions (2006)

The wide variety of services and resources available over the Internet presents new opportunities for organisations to collaborate to reach common goals. For example, business partners wish to access each other’s services and share information along the supply chain in order to compete more successfully in the delivery of goods or services to the ultimate customer. This can lead to the investment of significant resources by business partners in the resulting collaboration. In the context of such high value business-to-business (B2B) interactions it is desirable to regulate (monitor and control) the behaviour of business partners to ensure that they comply with agreements that govern their interactions. Achieving this regulation is challenging because, while wishing to collaborate, organisations remain autonomous and may not unguardedly trust each other. Two aspects must be addressed: (i) the need for high-level mechanisms to encode agreements (contracts) between the interacting parties such that they can be used for run-time monitoring and enforcement, and (ii) systematic support to monitor a given interaction for conformance with contract and to ensure accountability. This dissertation concerns the latter aspect — the definition, design and implementation of underlying middleware support for the regulation of B2B interactions. To this end, two non-repudiation services are identified — non-repudiable service invocation and non-repudiable information sharing. A flexible nonrepudiation protocol execution framework supports the delivery of the identified services. It is shown how the services can be used to regulate B2B interactions. The non-repudiation services provide for the accountability of the actions of participants; including the acknowledgement of actions, their run-time validation with respect to application-level constraints and logging for audit. The framework is realised in the context of interactions with and between components of a J2EE application server platform. However, the design is sufficiently flexible to apply to other common middleware platforms.
Large-Scale Isolation and Crystallization of Epothilone D From Myxococcus xanthus Cultures (2002)

The introduction of the epothilone polyketide synthase (PKS) into Myxococcus xanthus has enabled the heterologous production of epothilone D (1) on a large scale. To isolate this valuable product from the fermentation medium, an economical, scalable, and high-yielding purification process was developed. With the crystallization of 1 from a binary solvent system that consisted of ethanol and water, the product was recovered as white crystals with a final purity of > or =97% (w/w). This is the first reported crystallization of 1.
One step fabrication of aligned carbon nanotubes using gas rectifier (2022)

We report the one-step fabrication of aligned and high-quality carbon nanotubes (CNTs) using floating-catalyst chemical vapor deposition (FCCVD) with controlled fluidic properties assisted by a gas rectifier. The gas rectifier consists of one-dimensional straight channels for regulating the Reynolds number of the reaction gas. Our computational fluid dynamics simulation reveals that the narrow channels of the gas rectifier provide steady and accelerated laminar flow of the reaction gas. In addition, strong shear stress is induced near the side wall of the channels, resulting in the spontaneous formation of macroscopic CNT bundles aligned along the direction of the gas flow. After a wet-process using chlorosulfonic acid, the inter-tube voids inherently observed in as-grown CNT bundles are reduced from 16 to 0.3%. The resulting CNT fiber exhibits a tensile strength of 2.1 ± 0.1 N tex<sup>-1</sup> with a Young's modulus of 39 ± 4 N tex<sup>-1</sup> and an elongation of 6.3 ± 0.6%. FCCVD coupled with the strong shear stress of the reaction gas is an important pre-processing route for the fabrication of high-performance CNT fibers.
Beyond amyloid: a diverse portfolio of novel drug discovery programs for Alzheimer's disease and related dementias (2011)

Although the molecular mechanisms underlying the pathogenesis of Alzheimer's disease and other related neurodegenerative diseases remain unclear, accumulation of misfolded proteins, neuroinflammation, mitochondrial dysfunction and perturbed calcium homeostasis have been identified as key events leading to neuronal loss during neurodegeneration. Evidence for 'druggable' targets for each of these key mechanisms was presented by the Alzheimer's Drug Discovery Foundation-funded investigators at the 12th International Conference on Alzheimer's Drug Discovery, Jersey City, NJ, 26-27 September 2011 http://www.worldeventsforum.com/addf/addrugdiscovery.