gebhart/patrepeval huggingface dataset:
Coordinated activities of wild-type plus mutant EZH2 drive tumor-associated hypertrimethylation of lysine 27 on histone H3 (H3K27) in human B-cell lymphomas (2010)

EZH2, the catalytic subunit of the PRC2 complex, catalyzes the mono- through trimethylation of lysine 27 on histone H3 (H3K27). Histone H3K27 trimethylation is a mechanism for suppressing transcription of specific genes that are proximal to the site of histone modification. Point mutations of the EZH2 gene (Tyr641) have been reported to be linked to subsets of human B-cell lymphoma. The mutant allele is always found associated with a wild-type allele (heterozygous) in disease cells, and the mutations were reported to ablate the enzymatic activity of the PRC2 complex for methylating an unmodified peptide substrate. Here we demonstrate that the WT enzyme displays greatest catalytic efficiency (k(cat)/K) for the zero to monomethylation reaction of H3K27 and diminished efficiency for subsequent (mono- to di- and di- to trimethylation) reactions. In stark contrast, the disease-associated Y641 mutations display very limited ability to perform the first methylation reaction, but have enhanced catalytic efficiency for the subsequent reactions, relative to the WT enzyme. These results imply that the malignant phenotype of disease requires the combined activities of a H3K27 monomethylating enzyme (PRC2 containing WT EZH2 or EZH1) together with the mutant PRC2s for augmented conversion of H3K27 to the trimethylated form. To our knowledge, this is the first example of a human disease that is dependent on the coordinated activities of normal and disease-associated mutant enzymatic function.
Anti-glycation properties of Illicium verum Hook. f. fruit in-vitro and in a diabetic rat model (2022)

These results suggest that I. verum supplementation might help to reduce the burden of AGEs, and may have potential in preventing diabetes-associated complications.
Constitutive Expression of hrap Gene in Transgenic Tobacco Plant Enhances Resistance Against Virulent Bacterial Pathogens by Induction of a Hypersensitive Response (2002)

Hypersensitive response-assisting protein (HRAP) has been previously reported as an amphipathic plant protein isolated from sweet pepper that intensifies the harpin(Pss)-mediated hypersensitive response (HR). The hrap gene has no appreciable similarity to any other known sequences, and its activity can be rapidly induced by incompatible pathogen infection. To assess the function of the hrap gene in plant disease resistance, the CaMV 35S promoter was used to express sweet pepper hrap in transgenic tobacco. Compared with wild-type tobacco, transgenic tobacco plants exhibit more sensitivity to harpin(Pss) and show resistance to virulent pathogens (Pseudomonas syringae pv. tabaci and Erwinia carotovora subsp. carotovora). This disease resistance of transgenic tobacco does not originate from a constitutive HR, because endogenous level of salicylic acid and hsr203J mRNA showed similarities in transgenic and wildtype tobacco under noninfected conditions. However, following a virulent pathogen infection in hrap transgenic tobacco, hsr203J was rapidly induced and a micro-HR necrosis was visualized by trypan blue staining in the infiltration area. Consequently, we suggest that the disease resistance of transgenic plants may result from the induction of a HR by a virulent pathogen infection.
Testing 10-Bit A/D Converter with a Digital VLSI Tester. (1986)

Although an intramuscular injection of angiogenic cells to ischemic limbs with peripheral artery disease is a therapeutic option to rescue patients by augmenting neovascularization in the limbs, oxidative stress in the limbs may accelerate apoptosis of the injected cells and thereby reduce the therapeutic effect. In this study involving mice with ischemic lower limbs, whether daily oral administration of RTA-dh404, which is an activator of nuclear factor erythroid 2-related factor 2 (Nrf2) with antioxidant activity, could reduce oxidative stress in the limbs and suppress apoptosis of adipose-derived regenerative cells (ADRCs) injected in the limbs, eventually augmenting neovascularization in the limbs, was evaluated. The tissue expression of Nrf2 and concentrations of total antioxidant capacity and superoxide dismutase in the mice ischemic limbs were higher in the RTA-dh404-treated mice than in the control treated mice, and oxidative stress in the limbs of the RTA-dh404 treated mice was decreased. The day after an intramuscular injection of human ADRCs into ischemic lower limbs of immunodeficient mice, the number of apoptotic ADRCs in the ischemic limbs was decreased by approximately 25% in the RTA-dh404-treated mice compared to the control mice. Fourteen days after cell injection, neovascularization and the salvage ratio were increased by approximately 10% and 63%, respectively, in the ischemic limbs in the RTA-dh404-treated mice compared to the control mice. Pretreatment of ischemic limbs by daily oral administration of RTA-dh404 may augment the effect of therapeutic angiogenesis using an intramuscular injection of ADRCs into the ischemic limbs.
Identification of Quantitative Trait Loci responsible for embryonic lethality in mice assessed by ultrasonography (2009)

Recurrent Spontaneous Abortion (RSA) is a frequent pathology affecting 1 to 5% of couples. In approximately 50 % of cases, the aetiology is unknown suggesting a subtle interaction between genetic and environmental factors. Previous attempts to describe genetic factors using the candidate gene approach have been relatively unsuccessful due to the physiological, cellular and genetic complexity of mammalian reproduction. Indeed, fertility can be considered as a quantitative feature resulting from the interaction of genetic, epigenetic and environmental factors. Herein, we identified Quantitative Trait Loci (QTL) associated with diverse embryonic lethality phenotypes and the subsequent embryonic resorption in 39 inter-specific recombinant congenic mice strains, using in vivo ultrasound bio-microscopy. The short chromosomal intervals related to the phenotypes will facilitate the study of a restricted number of candidate genes which are potentially dysregulated in patients affected by RSA.
Possible impact of genetic differences on the development of neuropathic pain-like behaviors after unilateral sciatic nerve ischemic injury in rats (2001)

The development of neuropathic-like behaviors following unilateral ischemic injury to the sciatic nerve was examined and compared in four rat strains: Sprague--Dawley (SD), Wistar--Kyoto (WK), spontaneously hypertensive (SHR) and Dark--Agouti (DA). We have also compared two sub-strains of SD rats supplied from two different vendors (SD-BK and SD-DK). The responses to mechanical, heat or cold stimuli of both hind paws were measured before and regularly after injury for up to 10 weeks. Spontaneous paw lifting and changes in paw posture after nerve injury were also examined. Significant differences in basal sensitivity to mechanical or heat stimulation were seen among the four rat strain studied with SHR and DA rats being less sensitive than the SD and WK rats. All strains of rats developed bilateral mechanical allodynia and ipsilateral heat hyperalgesia after photochemically-induced nerve ischemia, but the time-course and magnitude of the responses were significantly different among the strains. Again, the SHR and DA were found to be least susceptible to the development of abnormal pain-like responses. Cold allodynia occurred only in WK and SD-BK. SD-DK rats on the other hand developed more severe mechanical allodynia than SD-BK. SHR and DA rats showed less deficits in paw posture after nerve injury whereas spontaneous pain lifting, a measure of possible spontaneous pain, was comparable among all strains. Light microscopic study of the injured sciatic nerve showed comparable nerve damage in SHR, WK and two sub-strains of SD rats. The DA rats however exhibited reduced area of intraneural damage. Finally, electronmicroscopic examination revealed that damage to both myelinated and unmyelinated fibers occurred in this model in all strains. These results showed that normal sensitivity and the development of pain-like response after partial nerve injury differ substantially among different strains of rats, supporting the emerging concept that genetic factors affect pain sensitivity under normal conditions and after nerve injury. The apparent resistance of DA rats to nerve ischemia, however, may suggest that genetic factors not directly related to pain modulation also play a role in the diverse outcomes. Our results indicate that sub-strains of rats also showed variable development of neuropathic pain-like behaviors to both the modality and magnitude of the effect. Thus, controlling sub-strains is also important in experimental studies of neuropathic pain in rats.
Observational Study of Spinal Muscular Atrophy Type 2 and 3 (2011)

Our results confirm previous clinical reports suggesting that SMA types 2 and 3 represent chronic phenotypes that have relatively stable clinical courses. We did not detect any measurable clinical disease progression in SMA types 2 and 3 over 12 months, suggesting that clinical trials will have to be designed to measure improvement rather than stabilization of disease progression.
Anti-Inflammatory Effect of Lupinalbin A Isolated from Apios americana on Lipopolysaccharide-Treated RAW264.7 Cells (2018)

Apios americana, a leguminous plant, is used as food in some countries. Although the biological activities of Apios extract have been reported, there have been no reports about the anti-inflammatory mechanism of lupinalbin A on the RAW264.7 cells. In this study, we investigated the anti-inflammatory effect of A. americana lupinalbin A on lipopolysaccharide (LPS)-treated RAW264.7 cells. Lupinalbin A significantly inhibited nitric oxide production and inducible nitric oxide synthase expression in LPS-treated RAW264.7 cells. The expression of cytokines, including interleukin-6, tumor necrosis factor-α, and chemokine of monocyte chemoattractant protein, was reduced under lupinalbin A exposure in LPS-treated RAW264.7 cells. In addition, lupinalbin A significantly decreased LPS-induced interferon (IFN)-β production and STAT1 protein levels in RAW264.7 cells. Taken together, these results suggest that A. americana lupinalbin A exerts anti-inflammatory effects via the inhibition of pro-inflammatory cytokines and blocking of IFN-β/STAT1 pathway activation.
On the Neuroprotective Effects of Naringenin: Pharmacological Targets, Signaling Pathways, Molecular Mechanisms, and Clinical Perspective (2019)

As a group of progressive, chronic, and disabling disorders, neurodegenerative diseases (NDs) affect millions of people worldwide, and are on the rise. NDs are known as the gradual loss of neurons; however, their pathophysiological mechanisms have not been precisely revealed. Due to the complex pathophysiological mechanisms behind the neurodegeneration, investigating effective and multi-target treatments has remained a clinical challenge. Besides, appropriate neuroprotective agents are still lacking, which raises the need for new therapeutic agents. In recent years, several reports have introduced naturally-derived compounds as promising alternative treatments for NDs. Among natural entities, flavonoids are multi-target alternatives affecting different pathogenesis mechanisms in neurodegeneration. Naringenin is a natural flavonoid possessing neuroprotective activities. Increasing evidence has attained special attention on the variety of therapeutic targets along with complex signaling pathways for naringenin, which suggest its possible therapeutic applications in several NDs. Here, in this review, the neuroprotective effects of naringenin, as well as its related pharmacological targets, signaling pathways, molecular mechanisms, and clinical perspective, are described. Moreover, the need to develop novel naringenin delivery systems is also discussed to solve its widespread pharmacokinetic limitation.
Stark and field-born resonances of an open square well in a static external electric field (2005)

The resonance positions, widths (inverse lifetimes), and wave functions of a square-potential well in the presence of a static electric field are calculated by using the outgoing boundary conditions. Our study concentrates on the field-born states that, unlike the well-known Stark resonances, are not associated with the field-free bound states. The effect of a lower cutoff of the static field on the field-born resonance phenomena is studied. The feasibility of experiments, where the isolated long-lived and overlapping short-lived field-born resonances can be explored, is discussed.
Nitrone cycloaddition in the stereoselective synthesis of .beta.-carbolines from N-hydroxytryptophan (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNitrone cycloaddition in the stereoselective synthesis of .beta.-carbolines from N-hydroxytryptophanRalf Plate, Pedro H. H. Hermkens, Jan M. M. Smits, and Harry C. J. OttenheijmCite this: J. Org. Chem. 1986, 51, 3, 309–314Publication Date (Print):February 1, 1986Publication History Published online1 May 2002Published inissue 1 February 1986https://pubs.acs.org/doi/10.1021/jo00353a007https://doi.org/10.1021/jo00353a007research-articleACS PublicationsRequest reuse permissionsArticle Views332Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Comparison of theAl27(He3,He3′)Al27andAl27(He3,t)Si27Reactions at 18 MeV (1973)

The $^{27}\mathrm{Al}$($^{3}\mathrm{He}$, $^{3}\mathrm{He}$\ensuremath{'})$^{27}\mathrm{Al}$ and $^{27}\mathrm{Al}(^{3}\mathrm{He},t)^{27}\mathrm{Si}$ reactions have been studied at an incident-beam energy of 18 MeV. Angular distributions were measured for many low-lying states. The transitions to states below 3.00 MeV excitation in the two reactions were compared using the macroscopic-interaction model of the distorted-wave Born approximation, using a generalized isospin-dependent optical-model potential. The observed $L=4$ strength in the ($^{3}\mathrm{He}$,$t$) reaction is inconsistent with both the inelastic scattering results of this work and other measurements of the hexadecapole deformation in this region, indicating that the inelastic scattering and charge-exchange processes may be microscopically more distinct than is suggested by the macroscopic model. Angular distributions for states in $^{27}\mathrm{Si}$ above 3 MeV excitation are also presented and discussed. The ($^{3}\mathrm{He}$,$t$) study also showed that the $^{27}\mathrm{Si}$ state of ${E}_{x}=4.46$ MeV is an unresolved doublet, confirming other recent work.
Episodic sampling: Towards energy-efficient patient monitoring with wearable sensors (2009)

Energy efficiency presents a critical design challenge in wireless, wearable sensor technology, mainly because of the associated diagnostic objectives required in each monitoring application. In order to maximize the operating lifetime during real-life monitoring and maintain sufficient classification accuracy, the wearable sensors require hardware support that allows dynamic power control on the sensors and wireless interfaces as well as monitoring algorithms to control these components intelligently. This paper introduces a context-aware sensing technique known as episodic sampling - a method of performing context classification only at specific time instances. Based on Additive-Increase/Multiplicative-Decrease (AIMD), episodic sampling demonstrates an energy reduction of 85 percent with a loss of only 5 percent in classification accuracy in our experiment.
Local Fisher embedding (2004)

In recent work, several supervised spectral embedding procedures have been proposed. Although experimentally validated, the construction of these algorithms was rather ad hoc. This paper shows how supervised locally linear embedding can be seen as performing a local Fisher mapping. A new formulation, combining linear and Fisher embedding, is proposed and experimentally validated.
<title>dc: a live Webcast control system</title> (2000)

Live Internet streaming media programs, called webcasts, can adopt techniques developed by television to obtain higher quality. We have developed a general webcast production model composed of three stages (i.e., sources, broadcast, and transmission) and a tool, called the Director's Console (dc), to control live webcasts. The tool is one component of a distributed service architecture, which adapts to varying physical infrastructure and broadcast configurations.
A case study: A leader's commitment to transparency and accountability through a serious reportable event (2013)

Analysis reveals that most preventable adverse events result from systemic causes, not human error. The senior patient care executive at a leading hospital recounts the unnecessary death of a patient and the investigation that followed. Citing the critical importance of a “just culture,” this case study offers a blueprint for managing a serious reportable event.
Association of HIV neutralizing antibody with lower viral load after treatment interruption in a prospective trial (A5170) (2012)

Nicastri et al.[1] raise an interesting point that perhaps the neutralizing antibody response is increased only against the autologous isolate because it is a ‘recall’ response and induction might be more rapid. We did not evaluate this in our study. There is convincing evidence indicating that autologous neutralizing antibodies lag behind virus evolution [2–6]. On the contrary, broadly neutralizing antibodies are often identified using heterologous isolates. Further, they are generally specific for more conserved regions of the envelope and have been associated with protection in nonhuman primates and are found in long-term nonprogressors and a fifth of all chronically infected persons [7–9]. We had previously reported increases in neutralizing antibody with long-term combination antiretroviral therapy [10]. We postulated that this might have an effect in controlling virus after discontinuation of combination antiretroviral therapy; however, we only saw an effect on viral load. It may be, as Nicastri et al.[1] suggest, that the use of both heterologous and autologous viruses might be a better measure of the breadth of neutralizing antibody responses to consider in the setting of structured treatment interruption. Acknowledgements Conflicts of interest There are no conflicts of interest.
Liver venous deprivation versus portal vein embolization before major hepatectomy: future liver remnant volumetric and functional changes (2020)

LVD is safe and provides greater FLR-V and FLR-F increase than PVE. These results are now evaluated in the HYPERLIV-01 multicenter randomized trial.
Cytofluorometric analysis of macrophages activated in vivo or in vitro (1978)

The relative RNA content of single macrophages was measured by cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. This method allowed the differentiation of two major groups in the adherent macrophage population differing in their RNA content. After in vivo stimulation of mice (by injection of thioglycollate medium) or guinea pigs (by injection of oil), an increasing percentage of macrophages possessing a high RNA content is recovered. In vitro stimulation of macrophage cultures with lipopolysaccharide had the same enhancing effect on cellular RNA content. Cytofluorometric measurement of RNA content may possibly become an efficient and rapid method of measuring macrophage activation.
Hardware lab tests on dynamic frequency support by converters applying different inertia constants (2017)

Today's electrical power systems are facing a continuous loss of conventional power plants and their mechanical inertia stabilizing the grid frequency during the first seconds of disturbance. Renewables and energy storages are usually converter connected and do not inherently provide any frequency support. Therefore, new converter control methods are required to maintain frequency stability despite increasing renewable penetration. This paper proposes a P(f)-droop control strategy for converters in which the droop parameters directly relate to the inertia constant in the grid. In this regard, high renewable penetration implies high frequency support by converters and vice versa. The concept was implemented and tested in a hardware lab with energy storages and converters and power ratings up to 100 kW. The test cases involved grid emulation with different inertia constants in order to analyze the impact of P(f)-droop-controlled converters on frequency dynamics. The test results showed that dynamic frequency deviations after a disturbance are significantly reduced with higher renewable penetration levels, when the loss of inertia is compensated by the proposed frequency support strategy.
The ERATO Systems Biology Workbench: An Integrated Environment for Multiscale and Multitheoretic Simulations in Systems Biology (2001)

Over the years, a variety of biochemical network modeling packages have been developed and used by researchers in biology. No single package currently answers all the needs of the biology community; nor is one likely to do so in the near future, because the range of tools needed is vast and&#13;\nnew techniques are emerging too rapidly. It seems unavoidable that, for the foreseeable future, systems biology researchers are likely to continue using multiple packages to carry out their work. &#13;\n In this chapter, we describe the ERATO Systems Biology Workbench (SBW) and the Systems Biology Markup Language (SBML), two related efforts directed at the problems of software package interoperability. The goal of the SBW project is to create an integrated, easy-to-use software environment that enables sharing of models and resources between simulation and analysis tools for systems biology. SBW uses a modular, plug-in architecture that permits easy introduction of new components. SBML is a proposed standard XML-based language for representing models communicated between software packages; it is used as the format of models communicated between components in SBW.
Synthesis of α‐Stereogenic Amides and Ketones by Enantioselective Conjugate Addition of 1,4‐Dicarbonyl But‐2‐enes (2009)

In the conjugate addition reaction of a alpha,beta-unsaturated compound, the new stereogenic center is created in the beta-position. In contrast, conjugate addition to 1,4-dicarbonyl but-2-enes will generate an alpha-stereogenic center with respect to one of the carbonyl groups, which informally, can be considered as an inversion of normal reactivity patterns or Umpolung. In this paper, we demonstrate that chiral bicyclic guanidine can catalyze the addition of 1,3-dicarbonyl compounds to 1,4-dicarbonyl but-2-enes [(E)-4-oxo-4-arylbutenamides and (E)-4-oxo-4-arylbutenones] with high regioselectivity and enantioselectivity (ee values up to 97%).