gebhart/patrepeval huggingface dataset:
Endogenous naive CD8 T cell precursor frequency and infection type control CD62L gene expression (85.6) (2007)

Abstract CD62L is essential for homing of lymphocytes to lymph nodes. Moreover, CD62L expression identifies distinct lineages of memory CD8 T cells. However, the factors regulating memory cell lineage differentiation are ill defined. Our data show that CD62L gene regulation is controlled by the input number of transferred antigen-specific CD8 T cells. To enable analysis of endogenous antigen-specific CD8 T cells, we have now utilized a method to enrich naïve antigen-specific CD8 T cells using MHC class I tetramers in conjunction with magnetic bead separation. Of three specificities tested thus far, naïve precursor frequencies ranged from ~100–1000 cells/mouse. Interestingly, following infection with recombinant vesicular stomatitis viruses, the naïve precursor frequency inversely correlated with the time required for the resulting memory population to shift to CD62L+ cells. Remarkably, in the case of ova-specific CD8 T cells, the shift toward CD62L+ ova-specific memory cells occurred substantially faster following infection with Listeria monocytogenes expressing ovalbumin than after VSV-ova infection. This system will allow us to examine parameters of the endogenous CD8 T cell response to infection from initial activation to memory development. This work was supported by NIH grants AI41576 and DK45260 (LL) and a Damon Runyon Foundation fellowship (KMK).
LRSpeech: Extremely Low-Resource Speech Synthesis and Recognition (2020)

Speech synthesis (text to speech, TTS) and recognition (automatic speech recognition, ASR) are important speech tasks, and require a large amount of text and speech pairs for model training. However, there are more than 6,000 languages in the world and most languages are lack of speech training data, which poses significant challenges when building TTS and ASR systems for extremely low-resource languages. In this paper, we develop LRSpeech, a TTS and ASR system under the extremely low-resource setting, which can support rare languages with low data cost. LRSpeech consists of three key techniques: 1) pre-training on rich-resource languages and fine-tuning on low-resource languages; 2) dual transformation between TTS and ASR to iteratively boost the accuracy of each other; 3) knowledge distillation to customize the TTS model on a high-quality target-speaker voice and improve the ASR model on multiple voices. We conduct experiments on an experimental language (English) and a truly low-resource language (Lithuanian) to verify the effectiveness of LRSpeech. Experimental results show that LRSpeech 1) achieves high quality for TTS in terms of both intelligibility (more than 98% intelligibility rate) and naturalness (above 3.5 mean opinion score (MOS)) of the synthesized speech, which satisfy the requirements for industrial deployment, 2) achieves promising recognition accuracy for ASR, and 3) last but not least, uses extremely low-resource training data. We also conduct comprehensive analyses on LRSpeech with different amounts of data resources, and provide valuable insights and guidances for industrial deployment. We are currently deploying LRSpeech into a commercialized cloud speech service to support TTS on more rare languages.
Penetrating duodenal trauma (2015)

Therapeutic/care management study, level IV.
Version adaptative d'un égaliseur DFE hybride en bloc utilisant la technique Overlap and Save (2013)

National audience
Intravascular Doppler technique for monitoring renal venous blood flow in man (2003)

The IVD technique applied to the venous side of the renal circulation provides a simple and reliable method for separate measurement of RBF in kidneys with and without renal artery stenosis.
RhoA activation and actin reorganization involved in endothelial CAM-mediated endocytosis of anti-PECAM carriers: critical role for tyrosine 686 in the cytoplasmic tail of PECAM-1 (2008)

Abstract Platelet-endothelial cell adhesion molecule-1 (PECAM-1), a transmembrane glycoprotein involved in leukocyte transmigration, represents a good target for endothelial drug delivery (eg, using antibody-directed nanocarriers, anti-PECAM/NCs). Although endothelial cells do not internalize PECAM antibodies, PECAM-1 engagement by multivalent anti-PECAM conjugates and nanocarriers causes endocytosis via a nonclassic CAM-mediated pathway. We found that endothelial uptake of multivalent anti-PECAM complexes is associated with PECAM-1 phosphorylation. Using model REN cells expressing a series of PECAM-1 deletion and point mutants, we found that the PECAM-1 cytoplasmic domain and, more precisely, PECAM-1 tyrosine 686, is critical in mediating RhoA activation and recruitment of EGFP-RhoA to anti-PECAM/NC binding sites at the plasmalemma, actin polymerization into phalloidin-positive stress fibers, and finally CAM endocytosis of anti-PECAM/NCs. Endothelial targeting and endocytosis of anti-PECAM/NCs were markedly efficient and did not compromise endothelial barrier function in vitro (determined by immunostaining of VE-cadherin and 125I-albumin transport across endothelial monolayers) or in vivo (determined by electron microscopy imaging of pulmonary capillaries and 125I-albumin transport from the blood into the lung tissue after intravenous injection of anti-PECAM/NCs in mice). These results reveal PECAM-1 signaling and interactions with the cytoskeleton, which are required for CAM-endocytosis, and may provide safe intra-endothelial drug delivery by anti-PECAM/NCs.
Synergistic therapeutic effects of cytokine-induced killer cells and temozolomide against glioblastoma (2010)

The presence of active immunity within the brain supports the possibility of effective immunotherapy for glioblastoma (GBM). To provide a clinically-relevant adoptive immunotherapy for GBM using ex vivo expanded cytokine-induced killer (CIK) cells, the treatment capability of CIK cells, either alone or in combination with temozolomide (TMZ) were evaluated. Human CIK (hCIK) cells were cultured from PBMC using activating anti-CD3 antibody and IL-2, which were 99% CD3+, 91% CD3+CD8+ and 29% CD3+CD56+. In vitro, hCIK cells showed tumor-specific cytotoxicity against U-87MG human GBM cells. When hCIK cells were injected into tail veins of immune-compromised mice bearing U-87MG tumors in their brains, numerous CIK cells infiltrated into the brain tumors. CIK treatments (1x10(5), 1x10(6) or 1x10(7), once a week for four weeks) inhibited the tumor growth significantly in a dose-dependent manner; 44, 54 and 72% tumor volume reduction, respectively, compared with the control group (P<0.05). Moreover, hCIK cells (1x10(7), once a week for four weeks) and TMZ (2.5 mg/kg, daily for 5 days) combination treatment further increased tumor cell apoptosis and decreased tumor cell proliferation and vessel density (P<0.05), creating a more potent therapeutic effect (95% reduction in tumor volume) compared with either hCIK cells or TMZ single therapy (72% for both, P<0.05). Taken together, CIK cell-immunotherapy and TMZ chemotherapy have synergistic therapeutic effects and could be combined for a successful treatment of GBM.
Estimation of gas-phase hydroxyl radical rate constants for organic chemicals (1988)

Abstract A structure—activity relationship developed for the estimation of rate constants for the gas-phase reactions of the hydroxyl radical with organic compounds is described and extended to certain organophosphorus compounds. The use of this estimation method is illustrated.
The "Vertigo Effect" on Your Smartphone: Dolly Zoom via Single Shot View Synthesis (2020)

Dolly zoom is a technique where the camera is moved either forwards or backwards from the subject under focus while simultaneously adjusting the field of view in order to maintain the size of the subject in the frame. This results in perspective effect so that the subject in focus appears stationary while the background field of view changes. The effect is frequently used in films and requires skill, practice and equipment. This paper presents a novel technique to model the effect given a single shot capture from a single camera. The proposed synthesis pipeline based on camera geometry simulates the effect by producing a sequence of synthesized views. The technique is also extended to allow simultaneous captures from multiple cameras as inputs and can be easily extended to video sequence captures. Our pipeline consists of efficient image warping along with depth-aware image inpainting making it suitable for smart-phone applications. The proposed method opens up new avenues for view synthesis applications in modern smart-phones.
Robust adaptive optimal control modification with large adaptive gain (2009)

A new adaptive control modification is presented that can achieve robust adaptation with a large adaptive gain without incurring high-frequency oscillations as with the standard model-reference adaptive control. The modification is based on an optimal control formulation that minimizes the L <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> norm of the tracking error. The optimality condition is used to derive the modification using the gradient method. The adaptive optimal control modification results in a stable adaptation and allows a large adaptive gain to be used for better tracking performance with improved stability robustness. Simulations demonstrate the effectiveness of the proposed modification.
Wastage and Self-Plugging by a Potential CO2 Ingress in a Supercritical CO2 Power Conversion System of an SFR (2010)

For a CO2 ingress accident into liquid sodium in a supercritical CO2 power conversion system coupled with a sodium-cooled fast reactor, we investigated two major design issues: i) a wastage phenomenon in regard to structural damage adjacent to the leaking position, and ii) potential channel plugging due to the formation of a particulate reaction product. In order to understand the factors affecting the occurrence of these issues, two kinds of experiments were carried out: a wastage effect test and a self-plugging test. All experimental conditions were chosen to reasonably represent the normal operating conditions and realistic design parameters of the reference plant. The test results indicate the absence of wastage, which will not lead to additional tube ruptures and damage propagation. In the current experiment, the self-plugging of PCHE channels only took place under two limited conditions: i) the sodium temperature is over 500°C and ii) the equivalent diameter of the crack opening is less than 1.5mm with a small leakage rate of far less than 1 g/s of CO2 ingress.
Antibodies to watch in 2022 (2022)

In this 13th annual installment of the annual 'Antibodies to Watch' article series, we discuss key events in commercial antibody therapeutics development that occurred in 2021 and forecast events that might occur in 2022. Regulatory review of antibody therapeutics that target the SARS-CoV-2 coronavirus proceeded at an unprecedented pace in 2021, resulting in both emergency use authorizations and full approvals for sotrovimab, regdanvimab, REGEN-COV2, as well as others, in numerous countries. As of November 1, a total of 11 antibody therapeutics had been granted first approvals in either the United States or European Union in 2021 (evinacumab, dostarlimab loncastuximab tesirine, amivantamab, aducanumab, tralokinumab, anifrolumab, bimekizumab, tisotumab vedotin, regdanvimab, REGEN-COV2). The first global approvals of seven products, however, were granted elsewhere, including Japan (pabinafusp alfa), China (disitamab vedotin, penpulimab, zimberelimab), Australia (sotrovimab, REGEN-COV2), or the Republic of Korea (regdanvimab). Globally, at least 27 novel antibody therapeutics are undergoing review by regulatory agencies. First actions by the Food and Drug Administration on the biologics license applications for faricimab, sutimlimab, tebentafusp, relatlimab, sintilimab, ublituximab and tezepelumab are expected in the first quarter of 2022. Finally, our data show that, with antibodies for COVID-19 excluded, the late-stage commercial clinical pipeline of antibody therapeutics grew by over 30% in the past year. Of those in late-stage development, marketing applications for at least 22 may occur by the end of 2022.
ChemInform Abstract: Efficient Intramolecular Cyclizations of Phenoxyethynyl Diols into Multisubstituted α,β‐Unsaturated Lactones. (2013)

Abstract In the presence of AgOTf as the catalyst the title cyclization proceeds under mild conditions to give α‐unsubstituted lactones.
Evaluation of Contrast Enhancement by Digital Equalization in Digital Mammography (2000)

Purpose: This study evaluated an algorithm based on a method of contrast enhancement by digital equalization (CEDE). Method: The algorithm was designed to enhance image contrast by employing digital equalization of digital mammograms. The CEDE algorithm was tested using ten mammograms with cancer (13 lesions) taken the University of South Florida data base, together with eight mammograms which only contained benign lesions. Three readers compared the processed images with the original mammograms for lesion conspicuity. A five point ranking scale was employed where a score of 3 corresponded to equal lesion visibility, ranks &gt; 3 corresponded to superior lesion visibility, whereas ranks &lt; 3 corresponded to markedly inferior lesion visibility. Results: The mean observer score for all lesions was always at least equal to that of the original digital mammogram (i.e., 3 or greater), and there was no evidence of any image distortion or other image processing artefacts. The mean rank (± standard deviation) for the 13 malignant lesions was 3.52 ± 0.38. The corresponding rank for the eight benign lesions was 3.33 ± 0.26. These differences were statistically significant in terms of standard error. Conclusion: The CEDE algorithm is capable of significantly enhancing lesion contrast in digital mammograms and our preliminary results indicate that this algorithm merits additional refinement and further (objective) evaluation.
An Orthogonal Similarity Reduction of a Matrix into Semiseparable Form (2005)

An algorithm to reduce a symmetric matrix to a similar semiseparable one of semiseparability rank 1, using orthogonal similarity transformations, is proposed in this paper. It is shown that, while running to completion, the proposed algorithm gives information on the spectrum of the similar initial matrix. In fact, the proposed algorithm shares the same properties of the Lanczos method and the Householder reduction to tridiagonal form. Furthermore, at each iteration, the proposed algorithm performs a step of the QR method without shift to a principal submatrix to retrieve the semiseparable structure. The latter step can be considered a kind of subspace-like iteration method, where the size of the subspace increases by one dimension at each step of the algorithm. Hence, when during the execution of the algorithm the Ritz values approximate the dominant eigenvalues closely enough, diagonal blocks will appear in the semiseparable part where the norm of the corresponding subdiagonal blocks goes to zero in the subsequent iteration steps, depending on the corresponding gap between the eigenvalues. A numerical experiment is included, illustrating the properties of the new algorithm.
The economic value of a new technology in growing potatoes: the hilling method (2013)

Combining finance knowledge, agricultural and farm machinery, this study analyzes the economic value of a new technology of growing potatoes-the hilling method compared to the traditional method without hilling. The authors find that this environmentally friendly technology has a large economic value measured in billions of Chinese yuans (CNY) for Shanxi Province in China. Our analysis also allows us to identify the breakeven price of around CNY 79,000 which is considerably higher than the current price. Therefore, the substantial potential profit margin is sustainable and creates a strong incentive for the technology's developers and business owners. The analysis in this study helps in determine the long-term economic value of the hilling method of potato farming. It also provides key information for policy makers as well as serves as a framework for valuating farming technology in general.
New Metallocene Catalyst Having an Indenyl Group and a Fluorenyl Group for Ethylene–Polar Monomer Copolymerization (2001)

Abstract New stereorigid bridged zirconocene having an indenyl group and a fluorenyl group as ligand was synthesized and investigated as an ethylene/1-hydroxy-10-undecene copolymerization catalyst. This catalyst using MAO as a cocatalyst promoted polymerization to produce copolymers having high molecular weight (Mw:121000) and high polar monomer content (36.7 mol%), displaying higher polymerization activities at higher temperature.
933. Closed-Chest Cell Injections into Mouse Myocardium Guided by High-Resolution Echocardiography (2005)

The mouse is an important model for the development of therapeutic stem cell implantation and DNA injection to treat ischemic myocardium. However, its small size hampers accurate implantation into the left ventricular (LV) wall. Precise positioning of injections requires surgical visualization of the heart, subject to complications and impractical for delayed or repeated injections. Furthermore, the thickness of the myocardium is comparable to the length of a needle bevel; so surgical exposure does not prevent inadvertent injection into the LV cavity. We describe the use of high resolution echocardiography to guide non-surgical injections accurately into the mouse myocardial wall. We developed and optimized this system using injections of a mixture of ultrasound contrast agent and fluorescent microspheres into the hearts of anesthetized mice, allowing us to learn how to interpret the ultrasound image of the needle in the heart during injection. We were successfully able to use this system in a mouse myocardial infarction model to target the injection of labeled cells to a region adjacent to the infarct. Intentional injection of tracer into the LV cavity resulted in a small accumulation of tracer in the myocardium, suggesting that non-guided cell injections into mouse hearts may appear to have been successful even if the majority of the injectate was lost in the chamber. Use of this system will allow precise injections of genes or cells into mouse myocardium by accurately guiding non-surgical injections to the desired locations in a clinically relevant time-frame.
Immobilization of yeast on delignified cellulosic material for room temperature and low-temperature wine making (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImmobilization of yeast on delignified cellulosic material for room temperature and low-temperature wine makingE. P. Bardi and A. A. KoutinasCite this: J. Agric. Food Chem. 1994, 42, 1, 221–226Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/jf00037a040https://doi.org/10.1021/jf00037a040research-articleACS PublicationsRequest reuse permissionsArticle Views592Altmetric-Citations98LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ultrastructural Evidence of Exosome Secretion by Progenitor Cells in Adult Mouse Myocardium and Adult Human Cardiospheres (2012)

The demonstration of beneficial effects of cell therapy despite the persistence of only few transplanted cells in vivo suggests secreted factors may be the active component of this treatment. This so-called paracrine hypothesis is supported by observations that culture media conditioned by progenitor cells contain growth factors that mediate proangiogenic and cytoprotective effects. Cardiac progenitor cells in semi-suspension culture form spherical clusters (cardiospheres) that deliver paracrine signals to neighboring cells. A key component of paracrine secretion is exosomes, membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane. Exosomes have been identified as the active component of proangiogenic effects of bone marrow CD34⁺ stem cells in mice and the regenerative effects of embryonic mesenchymal stem cells in infarcted hearts in pigs and mice. Here, we provide electron microscopic evidence of exosome secretion by progenitor cells in mouse myocardium and human cardiospheres. Exosomes are emerging as an attractive vector of paracrine signals delivered by progenitor cells. They can be stored as an "off-the-shelf" product. As such, exosomes have the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
Impact of aeration strategies on fed-batch cell culture kinetics in a single-use 24-well miniature bioreactor (2013)

The need to bring new biopharmaceutical products to market more quickly and to reduce final manufacturing costs is driving early stage, small scale bioprocess development. This work describes a comprehensive engineering characterisation of a novel, single-use 24-well parallel miniature bioreactor system. Cell culture performance is also investigated, with particular focus on the aeration strategies adopted at this small scale (7 mL) either by headspace sparging alone or by direct gas sparging into the culture medium. Apparent volumetric oxygen mass transfer coefficient (kLa) values ranged between 3–22 h−1 and 4–53 h−1 for headspace aeration and direct gas sparging respectively. The higher kLa values with direct gas sparging correlated directly with the increase in gas–liquid interfacial area per unit volume. Mixing times (tm) over a range of conditions were generally in the range 1–13 s and decreased with increasing shaking frequency (500–800 rpm). Direct gas sparging also served to reduce tm values by a factor of up to 19 fold. The impact of aeration strategies on cell culture kinetics of a model CHO cell line was also determined. Cultures performed with head space aeration alone showed the highest viable cell density (VCD) (15.2 × 106 cells mL−1), viability retention and antibody titre (1.58 g L−1). These were greater than in conventional shake flask cultures due to the improved control of the μ24 bioreactor system. In all cases the miniature bioreactor managed good control of process parameters such as pH 6.95 ± 0.4, temperature T°C 37 ± 0.4 and DO% 57 ± 32. Cultures performed with direct gas sparging showed a 25–45% reduction in VCD (depending on the aeration strategy used) and a similar reduction in antibody titre. Overall this work shows the successful application of the miniature bioreactor system for industrially relevant fed-batch cultures and highlights the impact of the dispersed gas phase on cell culture performance at the small scale.