gebhart/patrepeval huggingface dataset:
The electromigration investigation of Cu-Ni nanocomposites (2012)

The paper presents the electromigration behavior study of Cu-Ni nanocomposite for low-power electromagnetic microactuator fabrication. The nanocomposite is characterized based the striped with 50μm width and synthesized in the Cu plating bath with the 2g/L concentration of 50nm Ni nanopowders. About 2.03% Ni weight percentage in the Cu-Ni nanocomposite stripe is characterized using inductively coupled plasma mass spectrometer (ICP-MS). The drift velocity, critical length, critical product, and activation energy of the Cu-Ni nanocomposite are 565nm/hr, 14μm, 1714 A/cm, and 0.39eV respectively. In comparison with the values of Cu which are 88nm/hr, 20μm, 2365 A/cm, and 1.09eV, respectively, the poor electromigration behavior of the nanocomposite is dominated by the surface diffusion mechanism of Cu atoms due to the void formation in the interface between itself and the passivation oxide.
Correlates of 25-Hydroxyvitamin D among Chinese Breast Cancer Patients (2014)

In the largest study to date, the prevalence of low vitamin D status was high among Chinese breast cancer patients and associated with higher BMI, smoking, and lower physical activity. Our findings support careful monitoring of vitamin D status and recommendations for supplementation and other lifestyle modifications that may improve vitamin D status in breast cancer patients.
Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli (2016)

Disulfide bonds occurred in majority of secreted protein. Formation of correct disulfide bonds are must for achieving native conformation, solubility and activity. Production of recombinant proteins containing disulfide bond for therapeutic, diagnostic and various other purposes is a challenging task of research. Production of such proteins in the reducing cytosolic compartment of E. coli usually ends up in inclusion bodies formation. Refolding of inclusion bodies can be difficult, time and labor consuming and uneconomical. Translocation of these proteins into the oxidative periplasmic compartment provides correct environment to undergo proper disulfide bonds formation and thus achieving native conformation. However, not all proteins can be efficiently translocated to the periplasm with the help of bacterial signal peptides. Therefore, fusion to a small well-folded and stable periplasmic protein is more promising for periplasmic production of disulfide bonded proteins. In the past decades, several full-length proteins or domains were used for enhancing translocation and solubility. Here, protein fusion tags that significantly increase the yields of target proteins in the periplasmic space are reviewed.
Supplemental Figure 2A from Suppression of Colon Tumorigenesis in Mutant <i>Apc</i> Mice by a Novel PDE10 Inhibitor that Reduces Oncogenic β-Catenin (2023)

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Evaluation of kinetic uncertainty in numerical models of petroleum generation (2006)

Oil-prone marine petroleum source rocks contain type I or type II kerogen having Rock-Eval pyrolysis hydrogen indices greater than 600 or 300–600 mg hydrocarbon/g total organic carbon (HI, mg HC/g TOC), respectively. Samples from 29 marine source rocks worldwide that contain mainly type II kerogen (HI = 230–786 mg HC/g TOC) were subjected to open-system programmed pyrolysis to determine the activation energy distributions for petroleum generation. Assuming a burial heating rate of 1C/m.y. for each measured activation energy distribution, the calculated average temperature for 50% fractional conversion of the kerogen in the samples to petroleum is approximately 136 7C, but the range spans about 30C (121–151C). Fifty-two outcrop samples of thermally immature Jurassic Oxford Clay Formation were collected from five locations in the United Kingdom to determine the variations of kinetic response for one source rock unit. The samples contain mainly type I or type II kerogens (HI = 230–774 mg HC/g TOC). At a heating rate of 1C/m.y., the calculated temperatures for 50% fractional conversion of the Oxford Clay kerogens to petroleum differ by as much as 23C (127–150C). The data indicate that kerogen type, as defined by hydrogen index, is not systematically linked to kinetic response, and that default kinetics for the thermal decomposition of type I or type II kerogen can introduce unacceptable errors into numerical simulations. Furthermore, custom kinetics based on one or a few samples may be inadequate to account for variations in organofacies within a source rock. We propose three methods to evaluate the uncertainty contributed by kerogen kinetics to numerical simulations: (1) use the average kinetic distribution for multiple samples of source rock and the standard deviation for each activation energy in that distribution; (2) use source rock kinetics determined at several locations to describe different parts of the study area; and (3) use a weighted-average method that combines kinetics for samples from different locations in the source rock unit by giving the activation energy distribution for each sample a weight proportional to its Rock-Eval pyrolysis S2 yield (hydrocarbons generated by pyrolytic degradation of organic matter).
Neutron-activated holmium-166-poly (L-lactic acid) microspheres: a potential agent for the internal radiation therapy of hepatic tumors. (1991)

Biodegradable Poly(L-lactic acid) microspheres containing neutron-activable 165Ho were designed for internal radiation therapy of hepatic tumors. Spheres composed of Poly(L-lactic acid) (PLA) were prepared with excellent reproducibility containing up to 36% of a holmium complex. The prepared spheres were irradiated in a high neutron flux converting 165Ho to 166Ho (Emax = 1.84 MeV, half-life = 26.9 hr). Thus, these microspheres can be prepared under conditions that do not require the handling of a hazardous radionuclide, and then irradiated just prior to administration. In vitro studies in plasma (n = 6) revealed 97.3% (+/- 1.9) retention of 166Ho in the microspheres after 240 hr. PLA spheres administered via the portal vein in rabbits (n = 6) show 94.5% (+/- 3.4) retention of the original 166Ho activity in the liver after 6 days.
Unity in Variety--The Pan-Genome of the Chlamydiae (2011)

Chlamydiae are evolutionarily well-separated bacteria that live exclusively within eukaryotic host cells. They include important human pathogens such as Chlamydia trachomatis as well as symbionts of protozoa. As these bacteria are experimentally challenging and genetically intractable, our knowledge about them is still limited. In this study, we obtained the genome sequences of Simkania negevensis Z, Waddlia chondrophila 2032/99, and Parachlamydia acanthamoebae UV-7. This enabled us to perform the first comprehensive comparative and phylogenomic analysis of representative members of four major families of the Chlamydiae, including the Chlamydiaceae. We identified a surprisingly large core gene set present in all genomes and a high number of diverse accessory genes in those Chlamydiae that do not primarily infect humans or animals, including a chemosensory system in P. acanthamoebae and a type IV secretion system. In S. negevensis, the type IV secretion system is encoded on a large conjugative plasmid (pSn, 132 kb). Phylogenetic analyses suggested that a plasmid similar to the S. negevensis plasmid was originally acquired by the last common ancestor of all four families and that it was subsequently reduced, integrated into the chromosome, or lost during diversification, ultimately giving rise to the extant virulence-associated plasmid of pathogenic chlamydiae. Other virulence factors, including a type III secretion system, are conserved among the Chlamydiae to variable degrees and together with differences in the composition of the cell wall reflect adaptation to different host cells including convergent evolution among the four chlamydial families. Phylogenomic analysis focusing on chlamydial proteins with homology to plant proteins provided evidence for the acquisition of 53 chlamydial genes by a plant progenitor, lending further support for the hypothesis of an early interaction between a chlamydial ancestor and the primary photosynthetic eukaryote.
Impact of Cleaning Procedures on Adhesion of Living Cells to Three Abutment Materials (2017)

All cleaning methods provided comparable cell detachment forces for LS abutments. AP/PF or ultrasonic cleaning were the most suitable methods for strong cell adhesion on Zr. UD provided the best cell adhesion for Ti.
In vivofluorescence imaging for tissue diagnostics (1997)

Non-invasive fluorescence imaging has the potential to provide in vivo diagnostic information for many clinical specialties. Techniques have been developed over the years for simple ocular observations following UV excitation to sophisticated spectroscopic imaging using advanced equipment. Much of the impetus for research on fluorescence imaging for tissue diagnostics has come from parallel developments in photodynamic therapy of malignant lesions with fluorescent photosensitizers. However, the fluorescence of endogenous molecules (tissue autofluorescence) also plays an important role in most applications. In this paper, the possibilities of imaging tissues using fluorescence spectroscopy as a mean of tissue characterization are discussed. The various imaging techniques for extracting diagnostic information suggested in the literature are reviewed. The development of exogenous fluorophores for this purpose is also presented. Finally, the present status of clinical evaluation and future directions are discussed.
Quantitative mass spectrometry of technical polymers: a comparison of several ionization methods (1998)

The development of soft ionization methods such as matrix-assisted laser desorption/ionization (MALDI), electrospray ionization (ESI) and secondary ion mass spectrometry (SIMS) has led to an increased use of mass spectrometry in characterizing technical (synthetic) polymers. In this paper, we compare the relative performance of these three ionization methods for characterizing the molecu lar weights, polydispersities and quantification of relative amounts of polymer components in mixtures. Two polymers used in biomaterials, poly(dimethylsiloxane) and poly(ethylene glycol), are employed as the model polymer systems for our survey because of their well-defined molecular weights and importance as surfactants in biomaterials and because many of their surface and solutionphase properties are well understood. Matrix-assisted laser desorption/ionization can be used to examine the surface and bulk com position of biomaterials, whereas secondary ion mass spectrometry is used for examining monolayer and submonolayer coverage of polymers on surfaces and electrospray ionization is suited for examination of extractables from biomaterials. Secondary ion mass spectrometry and electrospray show discriminate behavior against ionization of higher molecular weight oligomers, especially of poly(dimethylsiloxane). Matrix-assisted laser desorption/ionization appears to exhibit the best performance for reliable molecular weight determination at higher masses and polydispersity characterization as well as for quantification of components in polymer mixtures. The results are discussed within the context of the ionization mechanisms by which each soft ionization technique operates and by the attributes of the mass analyzers (time-of-flight and Fourier transform mass spectrometers) employed.
Formation of Water-Free Cavity in the Process of Nafion Swelling in a Cell of Limited Volume; Effect of Polymer Fibers Unwinding (2020)

When Nafion swells in water, colloidal particles are repelled from the polymer surface; this effect is called the formation exclusion zone (EZ), and the EZ size amounts to several hundred microns. However, still no one has investigated the EZ formation in a cell whose dimension is close to the EZ size. It was also shown that, upon swelling in water, Nafion fibers "unwind" into the water bulk. In the case of a cell of limited volume, unwound fibers abut against the cell windows, and water is completely pushed out from the region between the polymer and the cell window, resulting in a cavity appearance. The temporal dynamics of the collapse of this cavity was studied depending on the cell size. It is shown that the cavity formation occurs due to long-range forces between polymer strands. It turned out that this scenario depends on the isotopic composition of the water, ionic additives and water pretreatment. The role of nanobubbles in the formation and collapse of the cavity were analyzed. The results obtained allowed us to conclude that the EZ formation is precisely due to the unwinding of polymer fibers into the liquid bulk.
A practical guide to the UNIX system: 2nd ed (1989)

A combination of physician billing and hospital discharge abstracts can be used to identify patients with IHD. Population prevalence of IHD can be measured using administrative data.
Preliminary evaluation of compressed air membrane dryers for exhibit case desiccation (1999)

An exhibition case displaying ancient bronzes was found to have a high leak-rate and was subject to a variety of environmental factors. Because of the original case design, passive means of control were not proving effective. A simple, low-output (below 28l.min−1) drying system based on hollow fibre (polysulfone) technology was tested and installed in the case to maintain the microenvironment below 43% relative humidity (RH). The membrane was tested below industrially-specified pressure parameters (less than 4 bar) and found to produce a range of consistent RH outputs suited to museum use. After some years in service, the system has proved reliable and simple to operate; optimum performance was achieved with more continuous operation.
Growth of Sn-Doped β-Ga2O3 Nanowires and Ga2O3−SnO2 Heterostructures for Gas Sensing Applications (2009)

Although β-Ga2O3 thin films and nanowires (NWs) show promise as very stable and reliable active components for high temperature gas sensors, their use at room temperatures is limited due to poor electrical conductivity. To address this problem, we grew Sn-doped β-Ga2O3 nanowires by the vapor−liquid−solid (VLS) approach. Sn-doped β-Ga2O3 NWs with diameters of 100−250 nm retained the monoclinic β-Ga2O3 structure, though photoluminescence (PL) emission was red-shifted by up to 50 nm relative to the deep defect band typically observed for pure β-Ga2O3 NWs. When higher amounts of Sn were introduced, individual Ga2O3−SnO2 heterostructures (HS) self-assembled, to form three distinctive parts: monocrystalline Sn-doped β-Ga2O3, poorly crystalline Sn-doped β-Ga2O3, and polycrystalline Ga-doped SnO2, thus realizing a p-n junction within a single HS. Factors responsible for the self-assembly of Ga2O3−SnO2 HS are the different vapor pressures of Sn and Ga and different growth kinetics of Ga2O3 and SnO2. Inhomogeneity in chemical content and structural composition correlated with distinct optical properties along the length of single HS. When diameters of these HS were less than 100 nm, Sn-doped Ga2O3 sections of the HS exhibited the rarely observed orthorhombic ε-Ga2O3 phase.
The crystal structure of the MPN domain from the COP9 signalosome subunit CSN6 (2012)

The COP9 signalosome (CSN) is a multiprotein complex containing eight subunits and is highly conserved from fungi to human. CSN is proposed to widely participate in many physiological processes, including protein degradation, DNA damage response and signal transduction. Among those subunits, only CSN5 and CSN6 belong to JAMM family. CSN5 possesses isopeptidase activity, but CSN6 lacks this ability. Here we report the 2.5Å crystal structure of MPN domain from Drosophila melanogaster CSN6. Structural comparison with other MPN domains, along with bioinformation analysis, suggests that MPN domain from CSN6 may serve as a scaffold instead of a metalloprotease.
Insulin analogues display IGF‐I‐like mitogenic and anti‐apoptotic activities in cultured cancer cells (2009)

Glargine, detemir and lispro, unlike regular insulin, exhibit in vitro proliferative and anti-apoptotic activities in a number of cancer cell lines. These actions resemble some of the effects of IGF-I, a growth factor involved in cancer initiation and progression. Insulin had no increased IGF-I activity. The specific receptor/s involved in mediating analogues actions remains to be identified.
Static Critical Behavior of the Spin-Freezing Transition in the Geometrically Frustrated Pyrochlore AntiferromagnetY2Mo2O7 (1997)

Some frustrated pyrochlore antiferromagnets, such as ${\mathrm{Y}}_{2}$M${\mathrm{o}}_{2}$${\mathrm{O}}_{7}$, show a spin-freezing transition and magnetic irreversibilities below a temperature ${T}_{f}$ similar to what is observed in randomly frustrated spin-glasses. We present results of nonlinear dc magnetization measurements on ${\mathrm{Y}}_{2}$M${\mathrm{o}}_{2}$${\mathrm{O}}_{7}$ that provide strong evidence that there is an underlying thermodynamic phase transition at ${T}_{f}$, which is characterized by critical exponents $\ensuremath{\gamma}\ensuremath{\approx}2.8$ and $\ensuremath{\beta}\ensuremath{\approx}0.8$. These values are typical of those found in random spin-glasses, despite the fact that the level of random disorder in ${\mathrm{Y}}_{2}$M${\mathrm{o}}_{2}$${\mathrm{O}}_{7}$ is immeasurably small.
Exon size and sequence conservation improves identification of splice-altering nucleotides (2019)

Pre-mRNA splicing is regulated through multiple <i>trans</i>-acting splicing factors. These regulators interact with the pre-mRNA at intronic and exonic positions. Given that most exons are protein coding, the evolution of exons must be modulated by a combination of selective coding and splicing pressures. It has previously been demonstrated that selective splicing pressures are more easily deconvoluted when phylogenetic comparisons are made for exons of identical size, suggesting that exon size-filtered sequence alignments may improve identification of nucleotides evolved to mediate efficient exon ligation. To test this hypothesis, an exon size database was created, filtering 76 vertebrate sequence alignments based on exon size conservation. In addition to other genomic parameters, such as splice-site strength, gene position, or flanking intron length, this database permits the identification of exons that are size- and/or sequence-conserved. Highly size-conserved exons are always sequence-conserved. However, sequence conservation does not necessitate exon size conservation. Our analysis identified evolutionarily young exons and demonstrated that length conservation is a strong predictor of alternative splicing. A published data set of approximately 5000 exonic SNPs associated with disease was analyzed to test the hypothesis that exon size-filtered sequence comparisons increase detection of splice-altering nucleotides. Improved splice predictions could be achieved when mutations occur at the third codon position, especially when a mutation decreases exon inclusion efficiency. The results demonstrate that coding pressures dominate nucleotide composition at invariable codon positions and that exon size-filtered sequence alignments permit identification of splice-altering nucleotides at wobble positions.
0.1 mu m contact metallization with SiH/sub 2/F/sub 2/-reduced CVD W (2003)

The fabrication of fine contact metallization down to 0.08 mu m in diameter for future 0.1- mu m-level ULSIs is discussed, and the electrical characteristics are evaluated. A two-layered etch mask, PMMA/poly-Si, was used for electron-beam delineation. Low-temperature dry etching permits the accurate patterning of the poly-Si layer in accordance with the PMMA mask, by increasing the PMMA etch-rate selectivity from 0.63 in ordinary dry etching to 15. Contact holes as small as 0.08 mu m in diameter are opened following application of the poly-Si as a mask. The combination of SiH/sub 2/F/sub 2/-reduced blanket CVD W (0.2 mu m thick)/sputtered W (30 nm thick) is used for metallization. Typical resistances are 1.5 k Omega on a 0.13- mu m-diameter contact to p/sup +/-Si and 107 Omega on a 0.18- mu m-diameter contact to n/sup +/-Si. Contact resistivities of these 0.1- mu m-level contacts are of the same levels as those of holes with diameters larger than 0.25 mu m.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Undoing Gender (2004)

Undoing Gender constitutes Judith Butler's recent reflections on gender and sexuality, focusing on new kinship, psychoanalysis and the incest taboo, transgender, intersex, diagnostic categories, social violence, and the tasks of social transformation. In terms that draw from feminist and queer theory, Butler considers the norms that govern--and fail to govern--gender and sexuality as they relate to the constraints on recognizable personhood. The book constitutes a reconsideration of her earlier view on gender performativity from Gender Trouble. In this work, the critique of gender norms is clearly situated within the framework of human persistence and survival. And to "do" one's gender in certain ways sometimes implies "undoing" dominant notions of personhood. She writes about the "New Gender Politics" that has emerged in recent years, a combination of movements concerned with transgender, transsexuality, intersex, and their complex relations to feminist and queer theory.
Voltage-dependent block by neomycin of the ATP-induced whole cell current of guinea-pig outer hair cells (1993)

1. The effects of externally applied ATP and neomycin on whole cell currents of isolated guinea pig cochlear outer hair cells (OHCs) were studied using the whole cell voltage-clamp technique. In OCHs held at -70 mV, ATP activated a large inward current. In the presence of neomycin, the ATP-induced whole cell current activated along a relatively unaltered time course, but the current then decreased to a reduced steady level. The neomycin inhibition of the ATP-induced current was dose dependent. The half-inhibitory concentration (IC50) of neomycin measured at steady state was estimated to be 90 microM. 2. Neomycin inhibition of the ATP response could not be reversed by increasing the concentration of ATP, indicating that the effect was noncompetitive. The inhibition was voltage dependent and was greatly reduced when OHCs were held at positive potentials. 3. Cells treated with 100 microM ATP gave maximal current responses. Addition of neomycin substantially increased membrane current noise of the 100 microM ATP responses. When neomycin concentration was varied from 10 to 500 microM, the current noise level peaked between 50 and 100 microM. The noise increase was observed at negative holding potentials but not at positive potentials. 4. The neomycin-induced whole cell current noise was used to estimate the size of the underlying elementary current. The ATP-induced single channel current of OHCs at -70 mV was estimated to be approximately 0.3 pA. The number of ATP-activated channels in a single OHC was estimated to be in the range of a few thousand. 5. The characteristics of the neomycin inhibition of ATP-induced currents were consistent with an open channel blocking mechanism. Analysis of the voltage dependence of the steady state neomycin inhibition suggested a neomycin binding site at an electrical distance of 0.3 from the extracellular side.