gebhart/patrepeval huggingface dataset:
Morphological and functional differences in coculture system of keratinocytes and dorsal-root-ganglion-derived cells depending on time of seeding (2011)

Previous study indicated that in a coculture system of keratinocytes and dorsal-root-ganglion-derived (DRG) cells, mechanical stimulation of keratinocytes induced ATP-mediated calcium propagation and excitation of DRG cells. Here, we examined two different coculture systems of keratinocytes and DRG cells. In one, we seeded keratinocytes first and then seeded DRG cells on the keratinocytes. In this system, nerve fibres from DRG cells passed between keratinocytes. Mechanical stimulation of keratinocytes did not induce excitation of DRG cells. In the other, we seeded both cell types together. At first, each cell type grew separately, forming cell aggregates. Then, nerve fibres grew out from the DRG cell aggregates to keratinocyte aggregates and penetrated into them. In this system, mechanical stimulation of keratinocytes induced excitation of the nerve fibres, but the excitation was not completely blocked by apyrase, an ATP-degrading enzyme. These results suggest that coculture of keratinocytes and DRG can generate a variety of structures, depending on the seeding conditions.
Inhibitory Control of Growth Hormone Secretion by Somatostatin in Rat Pituitary GC Cells: sst<sub>2</sub> but Not sst<sub>1</sub> Receptors Are Coupled to Inhibition of Single-Cell Intracellular Free Calcium Concentrations (2002)

Rat pituitary tumor cells (GC cells) exhibit spontaneous oscillations of intracellular free calcium concentration ([Ca(2+)](i)) that allow continuous release of growth hormone (GH). Of the somatostatin (SRIH) receptor subtypes (sst receptors) mediating SRIH action, sst(1) and sst(2) receptors are highly expressed by GC cell membranes. In the present study, the effects of sst(1) or sst(2) receptor activation on single-cell [Ca(2+)](i) were investigated in GC cells by confocal fluorescence microscopy. In addition, the effects of sst(1) or sst(2) receptor activation on GH secretion were also studied. Our results demonstrate that SRIH decreases [Ca(2+)](i) baseline and almost completely blocks Ca(2+) transients through activation of sst(2) but not of sst(1) receptors. In contrast, SRIH effectively inhibits GH secretion through activation of both sst(1) and sst(2) receptors. Blocking Ca(2+) transients is less efficient than SRIH to inhibit GH release. The cyclic octapeptide, CYN-154806, antagonizes sst(2) receptors at [Ca(2+)](i) since it abolishes the sst(2) receptor-mediated inhibition of [Ca(2+)](i) without affecting single-cell Ca(2+) signals. On the other hand, CYN-154806 alone potently inhibits GH secretion through the involvement of pertussis toxin-sensitive G proteins. In conclusion, the present results demonstrate that SRIH inhibition of GH release in GC cells involves mechanisms either dependent or independent on SRIH modulation of [Ca(2+)](i). The implications of CYN-154806 inhibition of GH secretion are discussed.
Activating Mutations of the Transmembrane Domain of MPL In Vitro and In Vivo: Incorrect Sequence of MPL-K, an Alternative Spliced Form of MPL (1998)

To the Editor:

Recently, many gene alterations have been identified as causes of leukemia, most of which are gross rearrangements of transcription factors, receptors, and kinases derived from chromosomal translocations. In addition, mutations of tyrosine kinase receptors such as c- kit 1 and FLT-3[
Pseudocapacitive Contributions to Electrochemical Energy Storage in TiO2 (Anatase) Nanoparticles (2007)

The advantages in using nanostructured materials for electrochemical energy storage have largely focused on the benefits associated with short path lengths. In this paper, we consider another contribution, that of the capacitive effects, which become increasingly important at nanoscale dimensions. Nanocrystalline TiO2 (anatase) was studied over a dimensional regime where both capacitive and lithium intercalation processes contribute to the total stored charge. An analysis of the voltammetric sweep data was used to distinguish between the amount of charge stored by these two processes. At particle sizes below 10 nm, capacitive contributions became increasingly important, leading to greater amounts of total stored charge (gravimetrically normalized) with decreasing TiO2 particle size. The area normalized capacitance was determined to be well above 100 μF/cm2, confirming that the capacitive contribution was pseudocapacitive in nature. Moreover, reducing the particle size to the nanoscale regime led to faster charge/discharge rates because the diffusion-controlled lithium ion intercalation process was replaced by faradaic reactions which occur at the surface of the material. The charge storage and kinetics benefits derived from using nanoscale metal oxides provide an interesting direction for the design of materials that offer both power density and energy density.
Colorectal cancer metastasis determined by carbohydrate-mediated cell adhesion: role of sialyl-LeX antigens. (1993)

Human colorectal carcinomas with increased metastatic potential and with poor prognosis are characterized by the high content of sialyl-LeX carbohydrate antigens. The levels of these carbohydrate antigens apparently increase during colorectal carcinoma progression from non-metastatic to metastatic tumors. The levels of tumor-associated sialyl-LeX antigens are inversely correlated to the post surgical survival of colon carcinoma patients as revealed by retrospective studies. Cell lines selected for high levels of cell surface sialyl-LeX antigens metastasize to livers when they are injected intrasplenically into nude mice. The highly expressing cells also strongly adhere to activated endothelial cells apparently through E-selectin. We conclude that sialyl-LeX carbohydrate antigen is a unique molecular phenotype that determines colorectal cancer metastasis.
Laser-Induced Breakdown Spectroscopy in a High-temperature and High-pressure Spray (2015)

Mixture composition in a spray formed by injecting n-heptane into high-temperature and high-pressure gas was measured by laser-induced breakdown spectroscopy (LIBS). The results show that LIBS has a potential for the measurement in a diesel spray.
5‐2: MEMS Mirror Based Dynamic Solid State Lighting Module (2016)

A novel dynamic solid state lighting (SSL) module is demonstrated, allowing users to program the direction, brightness, divergence and shape of a projected white light in a variety of lighting applications. The module is enabled by a fast beams‐teering MEMS mirror with high optical power handling. In order to produce and project bright white light, the module illuminates a phosphor target with a high power laser of several Watts. A projection lens projects the phosphor target into a cone of light of up to 60°. This light projection is arbitrarily shaped and dynamically programmable by controlling a MEMS mirror scan head to address specific portions of the proper target. An adjustable focus lens in the scan head further allows control of projected beam divergence. Successful prototypes were demonstrated with both a single 445nm laser diode source (2.5W laser power). While the prototypes have to date been limited by available power of the laser sources, it is estimated that the novel MEMS mirror scan head design allows &gt;10W (CW) operation on a 2mm without multilayer dielectric coatings. The introduction of dynamic laser beam steering is a paradigm shift toward a new generation of solid state lighting (SSL) with real‐time control of ”lighting content”.
Inhibition by Rapamycin of PDGF- and bFGF-Induced Human Tenon Fibroblast Proliferation in Vitro (1996)

Rapamycin potently inhibits platelet-derived growth factor-induced fibroblast proliferation in vitro without any apparent cytotoxicity. It may eventually prove to be a useful adjunct to glaucoma filtration surgery.
TxBox: Building Secure, Efficient Sandboxes with System Transactions (2011)

TxBox is a new system for sand boxing untrusted applications. It speculatively executes the application in a system transaction, allowing security checks to be parallelized and yielding significant performance gains for techniques such as on-access anti-virus scanning. TxBox is not vulnerable to TOCTTOU attacks and incorrect mirroring of kernel state. Furthermore, TxBox supports automatic recovery: if a violation is detected, the sand boxed program is terminated and all of its effects on the host are rolled back. This enables effective enforcement of security policies that span multiple system calls.
Cats produce analgesia in rats on the tail-flick test: naltrexone sensitivity is determined by the nociceptive test stimulus (1990)

Previous research has demonstrated that exposure to a cat produces a naltrexone-reversible antinociception as assessed in the formalin test in rats. Because different neurochemical mechanisms inhibit different forms of nociception, the present study examined whether presentation of a cat would also produce a naltrexone-reversible antinociception in the tail-flick response to radiant heat and electric shock. Exposure to the cat produced antinociception in both tail-flick paradigms. Naltrexone blocked the inhibition of the thermally evoked tail-flick response, but had no effect in the electric shock tail-flick paradigm. These results indicate that opioid mediation of stress-induced analgesia is determined, in part, by the nociceptive test employed.
Globalization and Consolidation of Peruvian Neoliberal States: From the Critical Theory Perspective (2021)

이 연구는 비판이론의 시각에서 초국가적 생산관계에 기초한 신자유주의 세계화와 이에 조응하는 ‘국가의 국제화’에 따른 페루 국가형태의 성격을 분석하고자 했다. 이를 위해 후지모리(1990-2000), 톨레도(2001-2006), 가르시아(2006-2011), 우말라(2011-2016) 정부를 중심으로 주요 정책, 정치이념 및 수사, 정치전략을 검토했다. 1990년 이후, 페루는 신자유주의 세계화의 주변부에서 권위적이고 배타적인 정치전략과 워싱턴 컨센서스로 대표되는 안정화 및 구조조정 정책을 추진했다. 그러나 온전한 헤게모니를 구축하지 못한 신자유주의는 많은 비판에 직면하게 되고 1990년대 중반 이후 좀 더 보편적인 동의를 획득하고자 ‘포섭적 신자유주의’로의 변화를 보였다. 이는 포스트 워싱턴 컨센서스로 구체화되었고 개별 국가 주도적 빈곤 감축 및 사회정책을 강조하는 정치적 전략의 결합으로 나타났다. 이 논문에서 살펴본 정부들의 정책적, 정치수사적 차별성은 이러한 변화에 조응하는 외양일 뿐이라 할 수 있다. 또한 페루의 사례는 배타적 신자유주의 국가에서 포섭적 신자유주의 국가로 전환되었을 때 오히려 경제주의적 신자유주의가 정치적, 사회적 영역까지 확장되고 제도화될 수 있음을 보여준다.
MDs bill for these routine nursing tasks (1991)

Georgia A. Moncada, RN, CPSN, is Clinical Coordinator for Specialties at Plastic, Hand, and Microsurgery, P.C in Pittsburgh, PA.
Cardanols detected in non-polar propolis extracts from Scaptotrigona aff. postica (Hymenoptera, Apidae, Meliponini) (2019)

Abstract The propolis produced by stingless bees of the tribe Meliponini is a viscous product that contains the resin collected from buds, leaves and plant exudates, mixed with salivary secretions, wax and soil. The species Scaptotrigona aff. postica (Latreille, 1807), (Hymenoptera, Apidae, Meliponinae) popularly known as “tubi” in Maranhão State, Brazil, does not mix soil to produce its propolis. The propolis from S. postica harvested in Barra do Corda, Maranhão State, is popularly used in the treatment of wounds and respiratory illnesses. The hydroalcoholic extract of this propolis, rich in flavone-6,8-di-C-glycosides (vicenin-2 and schaftoside), pyrrolizidine alkaloids derived from retronecine, catechin and caffeoylquinic acid derivatives exhibited antiviral activity against the herpes simplex and rubella viruses. The aim of this study was to increase knowledge about the chemical composition of the S. postica propolis by analyzing non-polar extracts obtained using hexane and chloroform as the solvents, by GC-EI-MS. A total of 15 constituents were identified comparing their respective mass spectral data with those available in the NIST data bases and those reported in the literature. The main constituents detected were the phenolic lipids, known as cardanols, 3-(4,7-heptadecadienyl) phenol (5), 3-(10-heptadecenyl) phenol (7), 3-heptadecylphenol (9) and 3-pentadecyl phenol or hydrocardanol (13), which predominated in the hexane extract, while the predominant constituents in the chloroform extract were 3-pentadecyl phenol or hydrocardanol (13) and 3-(8-pentadecenyl) phenol (12). The antioxidant, antitumoral, antifeedant, cytotoxic, anticarcinogenic, antiproliferative, antimicrobial, antileishmanial and larvicidal activities of the cardanols have been demonstrated in many studies.
Low-Power Circuits for the Bidirectional Wireless Monitoring System of the Orthopedic Implants (2009)

This paper proposes an architecture of the wireless monitoring system for the real-time monitoring of the orthopedic implants, which monitors the implant duty cycle, detects abnormal asymmetry, high amounts of force, and other conditions of the orthopedic implants. Data for diagnosis are communicated wirelessly by the radio-frequency (RF) signal between the embedded chip and the remote circuit. In different working modes, the system can be powered by the RF signal or stiff lead zirconate-titanate (PZT) ceramics which are able to convert mechanical energy inside the orthopedic implant into electrical energy. The power circuits with a variable ratio switched-capacitor (SC) dc-dc converter have been taped out with 0.35-mum complementary metal-oxide semiconductor (CMOS) technology. The test results show that the SC converter can transfer the input voltage that ranges from 5 V to 14 V from the PZT ceramics into the voltage ranging from 2 V to 2.5 V which will be dealt with by a low drop-out circuit in the future work. The total efficiency of the SC converter is from 28% to 42% at full-time working mode. The analog-to-digital converter (ADC) circuits have been fabricated in a 0.18-mum 1P6M CMOS process. The test results show that the ADC chip consumes only 12.5 muW in working mode and 150 nW in the sleep mode. The circuits, including RF circuits, ADC, and the microcontrol unit, have been implemented in a 0.18-mu m CMOS process. Future work includes some clinical experiments test in the application where PZT elements are used for power generation in total knee-replacement implants.
Hydrodesulfurization of petroleum distillates. [catalyst comprised of Group VI-B hydrogenating component, at least one iron group hydrogenating component, and alumina] (1975)

Sulfur-containing petroleum distillates are desulfurized with hydrogen and with a catalyst composite containing a Group VI-B hydrogenating component, at least one iron group hydrogenating component and alumina. The catalyst is prepared by calcining alumina having a bayerite content of 0-50 wt. percent, at a temperature of 800/sup 0/-1600/sup 0/F, impregnating the calcined alumina with the hydrogenating components and calcining the impregnatd alumina at 800/sup 0/-1600/sup 0/F. The composite catalyst is characterized as having a pore volume of at least 0.5 cc/g, a surface area of at least 150 m/sup 2//g and an average pore radius of 45 to 75 Angstrom units. At least 65 percent of the pore volume of the composite catalyst is in pores having a radius in the range of 50-300 Angstrom units. 4 Claims, No Drawings.
A Framework for Knowledge Management in Higher Education Using Social Networking (2012)

Recent years show an increase in the popularity of online social networking among the younger generation that opens up possibilities for educators to use it as a higher education platform. The focus of this paper is to highlight some open research questions in the context of knowledge management in higher education with the use of online social networking. Analysis of current research reveals that social networking sites are a useful tool in teaching and learning as well as in employability and career management of students. However, research is limited in terms of the applicability of social networking sites in other parts of the student life cycle including lifelong learning. The possibility of using online social networking in knowledge management, particularly in the area of knowledge accumulation and knowledge sharing is yet to be properly addressed by researchers. Therefore, the purpose of this paper is to draw attention to some implications of exploiting knowledge resources with online social networking for HE institutions.
A low power high power supply rejection ratio bandgap reference for portable applications (2008)

Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2008.
Diazirine-Based Multifunctional Photo-Probes for Affinity-Based Elucidation of Protein-Ligand Interaction (2014)

Diazirine-based photoaffinity labeling is recognized as one of the most reliable methods for analyzing protein-ligand interactions.Despite its excellent chemical and physical properties, the labeling process consists of an inefficient series of analytical steps: probe preparation, detection and isolation of the target protein, and identification of the labeled site within the active site.Firstly, this review introduces a versatile method for derivatization of trifluoromethylphenyldiazirine, the most common photocross-linker, then discusses its use in optimizing the probe for the simplification of labeled protein analyses including selective detection and purification, and the resulting acceleration of target protein identification by structural analysis.The present review reflects recent achievements in the chemistry and biological application of diazirine photoaffinity probes.CONTENTS 1.Chemical Approaches in the Analysis of Biomolecular Interaction 2. Photoaffinity Labeling 3. Diazirine Photophore for PAL-Based Protein Capture 3-1.Advantage over Other Photophores 3-2.Versatile Synthetic Intermediates Leading to a Library of Various Compounds 3-3.Probes for Biomolecules 4. Detection and Purification of Captured Proteins 4-1.General Features of PAL-Based Target Identification 4-2.Biotin Tag 4-3.Fluorescent Tag 4-4.Fluorous Tag 4-5.Cleavable Tag 4-6
Learning a correlated model of identity and pose-dependent body shape variation for real-time synthesis (2006)

We present a method for learning a model of human body shape variation from a corpus of 3D range scans. Our model is the first to capture both identity-dependent and pose-dependent shape variation in a correlated fashion, enabling creation of a variety of virtual human characters with realistic and non-linear body deformations that are customized to the individual. Our learning method is robust to irregular sampling in pose-space and identity-space, and also to missing surface data in the examples. Our synthesized character models are based on standard skinning techniques and can be rendered in real time.
Cancer phylogenetic tree inference at scale from 1000s of single cell genomes (2023)

A new generation of scalable single cell whole genome sequencing (scWGS) methods allows unprecedented high resolution measurement of the evolutionary dynamics of cancer cell populations. Phylogenetic reconstruction is central to identifying sub-populations and distinguishing the mutational processes that gave rise to them. Existing phylogenetic tree building models do not scale to the tens of thousands of high resolution genomes achievable with current scWGS methods. We constructed a phylogenetic model and associated Bayesian inference procedure, sitka, specifically for scWGS data. The method is based on a novel phylogenetic encoding of copy number (CN) data, the sitka transformation, that simplifies the site dependencies induced by rearrangements while still forming a sound foundation to phylogenetic inference. The sitka transformation allows us to design novel scalable Markov chain Monte Carlo (MCMC) algorithms. Moreover, we introduce a novel point mutation calling method that incorporates the CN data and the underlying phylogenetic tree to overcome the low per-cell coverage of scWGS. We demonstrate our method on three single cell datasets, including a novel PDX series, and analyse the topological properties of the inferred trees. Sitka is freely available at https://github.com/UBC-Stat-ML/sitkatree.git
Multi-robot Localization and Mapping Based on Signed Distance Functions (2015)

This publication describes a 2D Simultaneous Localization and Mapping approach applicable to multiple mobile robots. The presented strategy uses data of 2D LIDAR sensors to build a dynamic representation based on Signed Distance Functions. A multi-threaded software architecture performs registration and data integration in parallel allowing for drift-reduced pose estimation of multiple robots. Experiments are provided demonstrating the application with single and multiple robot mapping using simulated data, public accessible recorded data as well as two actual robots operating in a comparably large area.
TGF-Beta Downregulation of Distinct Chloride Channels in Cystic Fibrosis-Affected Epithelia (2014)

TGF-beta is sufficient to downregulate two critical chloride transporters in two CF-affected tissues that precedes expression changes of two distinct TGF-beta regulated proteins. Our results provide a plausible mechanism for CF-disease modification by TGF-beta through effects on CaCC.
Determination of Caffeine, Theophylline and Theobromine in Serum and Saliva Using High-Performance Liquid Chromatography (1984)

A method is described for the measurement of theobromine, theophylline and caffeine in serum and saliva by high-performance liquid chromatography (HPLC). A chloroform/isopropanol extract (85:15, v/v) is evaporated to dryness and chromatographed on a 100 X 4.5 mm id Hypersil octadecylsilane column with UV detection at 280 nm. Theobromine, theophylline, caffeine and the internal standard proxyphylline are satisfactorily resolved with an elution system of acetonitrile/tetrahydrofuran/50 mM acetate buffer, pH 4.0, (4:1:95, v/v). No interference is observed from the presence of xanthine metabolites or any of a number of common drugs examined. A good correlation was observed between the concentrations of caffeine in serum and in saliva suggesting that salivary measurements may be useful for the study of caffeine pharmacokinetics in man. Caffeine levels determined by the HPLC procedure described here agreed well with those obtained by a radioimmunoassay method. The method is also suitable for determining the xanthine content of beverages by direct injection of diluted samples.
Low power self refresh mode DRAM with temperature detecting circuit (1993)

To reduce self refresh mode current, a temperature detecting circuit, back bias generator and voltage down convertor, are developed. Using these circuits in a 16M DRAM, 33 /spl mu/A consuming current in self refresh mode has been realized at Vcc=5V, Ta=25 /spl deg/C.
Matrix metalloproteinases and processing of pro-TNF-α (1995)

Tumor necrosis factor-alpha (TNF-alpha) is released from a cell membrane-anchored precursor by proteolytic cleavage. We have shown that broad spectrum synthetic inhibitors of matrix metalloproteinases (MMPs) prevent the processing of the TNF precursor but do not inhibit the release of other cytokines. Purified MMPs, stromelysin, matrilysin, collagenase, and the gelatinases can all cleave a recombinant pro-TNF substrate to yield mature TNF. MMP inhibitors prevent the rise in blood levels of TNF after endotoxin administration in rats and are effective in animal models of inflammatory disease such as adjuvant arthritis. Drugs that inhibit MMP action and TNF release show great promise for the treatment of autoimmune inflammatory diseases.