gebhart/patrepeval huggingface dataset:
A novel approach to generating disease modifying dendritic cells (DCs) (129.13) (2007)

Abstract Dendritic cells (DCs) bridge the innate and adaptive immune responses and DC immunotherapy has shown promise in the treatment of transplantation, allergic and autoimmune diseases. One primary limitation in studying and utilizing DCs is their small numbers in vivo. While the growth factor Flt3-ligand (FL) is used to expand DCs in vivo, GM-CSF is predominantly used to generate DCs in vitro from bone marrow (BM-DC). The purpose of this study was to explore the use of murine FL (mFL), the mouse homologue of human FL, to generate DCs in vitro. In vivo (tumor-based) mFL delivery reliably expanded splenic DCs comparable to hFL In vitro BM-DCs generated with mFL were more similar to hFL than GM-CSF derived BM-DCs and appeared to be immature. Specifically, CD11c, CD11b and maturation markers CD80 and CD86 were lower in mFL and hFL versus GM-CSF BM-DCs. Maturation of mFL BM-DCs with TNF-α±LPS increased CD80, CD86 and CD40 expression. Transfer of matured mFL BM-DCs to mice prior to immunization with myelin oligodendrocyte glycoprotein (MOG35-55) significantly increased cumulative disease (26.79±10.12) and peak disease scores (3.07±1.16) compared to immature mFL BM-DCs (20.21 ±7.64 and 1.86±0.7, respectively). These results suggest that exposure to mFL in vitro is a novel method to generate large numbers of immature BM-DCs which can modulate the course of autoimmune disease. (Support: NIH AI-43376, K01 R022198 and NMSS RG3272).
QUORUM SENSING: Cell-to-Cell Communication in Bacteria (2005)

Bacteria communicate with one another using chemical signal molecules. As in higher organisms, the information supplied by these molecules is critical for synchronizing the activities of large groups of cells. In bacteria, chemical communication involves producing, releasing, detecting, and responding to small hormone-like molecules termed autoinducers . This process, termed quorum sensing, allows bacteria to monitor the environment for other bacteria and to alter behavior on a population-wide scale in response to changes in the number and/or species present in a community. Most quorum-sensing-controlled processes are unproductive when undertaken by an individual bacterium acting alone but become beneficial when carried out simultaneously by a large number of cells. Thus, quorum sensing confuses the distinction between prokaryotes and eukaryotes because it enables bacteria to act as multicellular organisms. This review focuses on the architectures of bacterial chemical communication networks; how chemical information is integrated, processed, and transduced to control gene expression; how intra- and interspecies cell-cell communication is accomplished; and the intriguing possibility of prokaryote-eukaryote cross-communication.
Singlet exciton fission via an intermolecular charge transfer state in coevaporated pentacene-perfluoropentacene thin films (2019)

Singlet exciton fission is a spin-allowed process in organic semiconductors by which one absorbed photon generates two triplet excitons. Theory predicts that singlet fission is mediated by intermolecular charge-transfer states in solid-state materials with appropriate singlet-triplet energy spacing, but direct evidence for the involvement of such states in the process has not been provided yet. Here, we report on the observation of subpicosecond singlet fission in mixed films of pentacene and perfluoropentacene. By combining transient spectroscopy measurements to nonadiabatic quantum-dynamics simulations, we show that direct excitation in the charge-transfer absorption band of the mixed films leads to the formation of triplet excitons, unambiguously proving that they act as intermediate states in the fission process.
Targeting the USP7/RRM2 axis drives senescence and sensitizes melanoma cells to HDAC/LSD1 inhibitors (2022)

Deubiquitinating enzymes are key regulators of the ubiquitin-proteasome system and cell cycle, and their dysfunction leads to tumorigenesis. Our in vivo drop-out screens in patient-derived xenograft models identify USP7 as a regulator of melanoma. We show that USP7 downregulation induces cellular senescence, arresting melanoma growth in vivo and proliferation in vitro in BRAF- and NRAS-mutant melanoma. We provide a comprehensive understanding of targets and networks affected by USP7 depletion by performing a global transcriptomic and proteomics analysis. We show that RRM2 is a USP7 target and is regulated by USP7 during S phase of the cell cycle. Ectopic expression of RRM2 in USP7-depleted cells rescues the senescent phenotype. Pharmacological inhibition of USP7 by P5091 phenocopies the shUSP7-induced senescent phenotype. We show that the bifunctional histone deacetylase (HDAC)/LSD1 inhibitor domatinostat has an additive antitumor effect, eliminating P5091-induced senescent cells, paving the way to a therapeutic combination for individuals with melanoma.
Joint linkage and association mapping of complex traits in shrub willow (Salix purpurea L.) (2019)

This study provides the first comparison of linkage analysis and linkage disequilibrium mapping approaches in Salix, and highlights the complementarity and limits of these two methods for elucidating the genetic architecture of complex bioenergy-related traits of a woody perennial breeding programme.
Synthesis of β‐Substituted γ‐Aminobutyric Acid Derivatives through Enantioselective Photoredox Catalysis (2018)

Abstract β‐Substituted chiral γ‐aminobutyric acids feature important biological activities and are valuable intermediates for the synthesis of pharmaceuticals. Herein, an efficient catalytic enantioselective approach for the synthesis of β‐substituted γ‐aminobutyric acid derivatives through visible‐light‐induced photocatalyst‐free asymmetric radical conjugate additions is reported. Various β‐substituted γ‐aminobutyric acid analogues, including previously inaccessible derivatives containing fluorinated quaternary stereocenters, were obtained in good yields (42–89 %) and with excellent enantioselectivity (90–97 % ee ). Synthetically valuable applications were demonstrated by providing straightforward synthetic access to the pharmaceuticals or related bioactive compounds ( S )‐pregabalin, ( R )‐baclofen, ( R )‐rolipram, and ( S )‐nebracetam.
Adaptive speech enhancement using frequency-specific SNR estimates (2002)

We describe an adaptive speech enhancement technique based on selecting a set of pre-computed FIR filters to process the compressed short-time power spectral trajectories of noisy speech. The responses of the pre-computed filters depend only on the signal to noise ratios (SNRs) and do not depend on the center frequency of the subbands. This allows for a compact design in which the estimate of the SNR at the particular frequency channel is used as the filter selection criterium for that sub-band.
Demystifying machine learning for mortality prediction (2021)

With interest, we read the article by Banoei et al. [1] on machine learning (ML) models to predict mortality among COVID-19 patients. They refer to other studies that failed to predict mortality using ‘conventional statistical analysis’, after which they present a linear ML model as a better suited method for such complex medical problems. We feel such a claim creates an image around ML as an alternative technique that offers solutions where statistical modeling fails. However, ML and statistical modeling are tightly interwoven. [...]
Atomic Emission Spectra of Xenon, Krypton, and Neon: Spectra from 200 to 900 nm by Sealed Inductively Coupled Plasma/Atomic Emission Spectroscopy (1994)

The emission spectra of pure xenon, krypton, and neon are reported over the spectral range of 200 to 900 nm from an enclosed inductively coupled plasma discharge operated at atmospheric pressure and 350 W.
Ionic liquids and continuous flow processes: a good marriage to design sustainable processes (2015)

In the last few years the use of Ionic Liquids (ILs) as alternative solvents for (bio)catalytic processes has increased substantially, and the benefits and different approaches reported to combine continuous flow systems and ILs are at the core of this overview.
Characterization of retroviruses from patients with multiple sclerosis (1997)

These studies were performed to characterize retroviruses found in cell lines spontaneously developed from peripheral blood mononuclear cells (PBMNC) from 6 multiple sclerosis patients, a patient with progressive myelopathy and a healthy control. The cell lines are B-lymphoblastoid and produce Epstein-Barr virus (EBV) particles or express EBV proteins. The B-lymphoblastoid cell lines are also characterized by production of low, fluctuating amounts of retrovirus. The low productivity complicates purification and characterization, but implementation of product-enhanced reverse transcriptase (PERT) assays has provided a highly useful tool for monitoring retrovirus production. By electron microscopy, the retroviral particles appear type-C-like. Functional assays indicate the presence of Pol, Gag and Env. Indirect ELISA demonstrates a significant relation between disease activity and reactivity towards retroviral peptides. Molecular characterization is primarily based on RT-PCR, cloning, sequencing and Northern- or Southern analyses. Molecular characterization is continuing.
Self-Supervised Learning of Depth and Ego-motion with Differentiable Bundle Adjustment (2019)

Learning to predict scene depth and camera motion from RGB inputs only is a challenging task. Most existing learning based methods deal with this task in a supervised manner which require ground-truth data that is expensive to acquire. More recent approaches explore the possibility of estimating scene depth and camera pose in a self-supervised learning framework. Despite encouraging results are shown, current methods either learn from monocular videos for depth and pose and typically do so without enforcing multi-view geometry constraints between scene structure and camera motion, or require stereo sequences as input where the ground-truth between-frame motion parameters need to be known. In this paper we propose to jointly optimize the scene depth and camera motion via incorporating differentiable Bundle Adjustment (BA) layer by minimizing the feature-metric error, and then form the photometric consistency loss with view synthesis as the final supervisory signal. The proposed approach only needs unlabeled monocular videos as input, and extensive experiments on the KITTI and Cityscapes dataset show that our method achieves state-of-the-art results in self-supervised approaches using monocular videos as input, and even gains advantage to the line of methods that learns from calibrated stereo sequences (i.e. with pose supervision).
Impacts of Software Agents in eCommerce Systems on Customer's Loyalty and on Behavior of Potential Customers (2011)

Most of the software agents only perform simple product price comparisons; some support the purchase of products or the negotiation over multiple terms of a transaction, such as, e. g., warranties, return policies, delivery times and loan options. Auctions help to find an effective pricing mechanism in electronic commerce. The active technologies enabling customers to purchase efficiently force the merchants to offer high personalized, value-added and complementary services. The techniques such as rule-based matching or collaborative filtering provide contents that are appropriate to the customer’s preferences or analyze past purchases of other clients. The one-to-one marketing may be especially useful for sophisticated products demanding explanation or to enable cross-selling of other products. The merchants might achieve additional reduction of transaction costs (especially transport, storage and safety measures) using electronic money systems.
Enhancing Enzyme Stability Against TiO2-UV Induced Inactivation (2004)

The use of enzymes in conjunction with inorganic photocatalysts requires stability against photooxidation. In this paper, we describe enhanced stabilization of a model enzyme, chymotrypsin, to photooxidation driven by titanium dioxide exposed to ultraviolet light (TiO(2)-UV). Stabilization is achieved conjugating the enzyme with an oligomeric adduct of UV-absorbing (2-[3-(2H-benzotriazol-2-yl)-4-hydroxyphenyl]ethyl methacrylate) (HBMA) and free radical-absorbing 2-methacryloyloxyethyl-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylate (Trolox-HEMA). Juxtaposition of the antioxidant Trolox with the UV absorber HBMA within a single chain reduced the rate of deactivation of the former by TiO(2)-UV. This enables modified enzyme, which is adsorbed on TiO(2), to absorb both UV-light and free radicals and locally reduce the rate of photooxidation. Interestingly, Trolox was more readily deactivated by TiO(2)-UV when it was conjugated separately to chymotrypsin that had been pre-modified with HBMA moieties.
Accuracy and performance of LACIE crop development models (1979)

Of the three principal phenological crop calendar models evaluated for LACIE, Robertson's triquadratic model which predicts the rate of progression of wheat through its biological development, was selected. Daily maximum and minimum temperatures and day length are the input variables, and the principal output is a daily increment of development through six physiological growth stages. Because wheat corresponds differently to the environment during each growth stage, five different equations are required. The estimated and observed crop development data were compared in order to establish a measure of confidence in the model and to identify consistent discrepancies that would adversely affect LACIE operation. Although the model provided reliable estimates for various wheat growing regions of the world, it was found that there are still areas in need of further model improvement or development.
Coating Produced by Deposition of Nanoparticles Blended Wires (2011)

Gas shield electric arc coating of carbon steel surface is considered in relation to repair applications. Two types of wires are incorporated in the experiments including standard and nano-particle blended. The coating structure and the metallurgy are examined using the Scanning Electron Microscopy (SEM), and Energy Dispersive Spectroscopy (EDS). The microhardness of the resulting coating is measured and compared with the results obtained from the conventional wire. It is found that fine structures are formed in the coating due to the presence of nanoparticles. In this case, microhardness increases significantly in the coating.
Isolation and Structure of Sesangolin, a Constituent of Sesamum angolense (Welw.) (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation and Structure of Sesangolin, a Constituent of Sesamum angolense (Welw.)William A. Jones, Morton Beroza, and Edwin D. BeckerCite this: J. Org. Chem. 1962, 27, 9, 3232–3235Publication Date (Print):September 1, 1962Publication History Published online1 May 2002Published inissue 1 September 1962https://pubs.acs.org/doi/10.1021/jo01056a058https://doi.org/10.1021/jo01056a058research-articleACS PublicationsRequest reuse permissionsArticle Views164Altmetric-Citations40LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Chemotherapy-related hyperbilirubinemia in pediatric acute lymphoblastic leukemia: a genome-wide association study from the AIEOP-BFM ALL study group (2023)

gov ; #NCT00430118.
The Spectrum of Constipation-Predominant Irritable Bowel Syndrome and Chronic Idiopathic Constipation: US Survey Assessing Symptoms, Care Seeking, and Disease Burden (2015)

CIC respondents with abdominal symptoms experience greater disease burden compared with CIC respondents without frequent abdominal symptoms and have a disease burden profile that is similar to IBS-C respondents.
Constructing Web-based legacy index card archives-architectural design issues and initial data acquisition (2002)

Presents a progress report (after 1 year of a 3 year project) on the overall design for a flexible archive conversion system, intended eventually for widespread use as a tool to convert legacy typescript and handwritten archive card indexes into Internet-accessible and searchable databases. The VIADOCS system is being developed and evaluated on a demonstrator archive of 30,000 pyraloid moth cards at the UK Natural History Museum, and has already demonstrated a successful and efficient mechanism for image acquisition using a modified bank cheque scanner. Document image processing and analysis techniques, defined by an XML validating document type definition (DTD), are being used to correct defects in the acquired images and parse card sequences to match the hierarchical taxonomy of pyraloid moth species. Parsed data is processed by offline OCR engines augmented by field-specific subject dictionaries to produce a 'draft' online archive. This archive will then be validated interactively via a Web browser as it is used. It is hoped eventually to provide an efficient and configurable legacy archive document conversion system not only for the Natural History Museum, but also for all museums, libraries and archives where there is a need to interrogate legacy documents via computer.
ClKβ(K-V) emission of CFCl3excited by synchrotron radiation below and above Cl-1sbinding energy: perturbation effects in a highly excited neutral molecule (1987)

The Cl Kβ (K-V) fluorescent emission and K absorption spectra from CFCl3 vapor were measured using monochromatic synchrotron radiation. Weak supra-threshold features were observed in the Cl K absorption spectrum. The Cl 1s binding energy of CFCl3 was estimated to be 2829.4eV. A comparison of the Cl Kβ (K-V) emission spectrum excited at 2833.5eV (K + 4eV) photon energy with the Kβ spectrum excited at 2880eV (K + 50eV) photon energy indicated an increase of emission intensity from multiple vacancy transitions. The Cl K absorption has a discrete absorption maximum (or a "white-line") at 2822.6eV, the transition of a Cl 1s electron to the first allowed unoccupied MO. For the first time an X-ray emission spectrum was obtained with excitation below the ionization threshold. Features in the 2822.6eV excited (an exciton state, i.e., a highly excited state of the neutral molecule) Cl Kβ (K-V) spectrum were observed to have significant intensity changes and relative energy position shifts from the emission spectrum excited above the Cl 1s threshold. These differences are explained in terms of perturbation effects due to the presence of an electron in the first unoccupied MO.
EXTH-43. GENERATION OF A B-CELL-BASED VACCINE FOR THE TREATMENT OF GLIOBLASTOMA (2020)

Abstract Immunotherapy has revolutionized the treatment of many tumors. However, most glioblastoma (GBM) patients have not, so far, benefited from such successes. With the goal of exploring ways to boost anti-GBM immunity, we developed a B-cell-based vaccine (BVax) that consists of 4-1BBL+ B cells activated with CD40 agonism and IFNg stimulation. BVaxmigrate to key secondary lymphoid organs and are proficient at antigen cross-presentation, which promotes both the survival and functionality of CD8+ T cells. A combination of radiation, BVax, and PD-L1 blockade conferred tumor eradication in 80% of treated tumor-bearing animals. This treatment elicited immunologic memory that prevented the growth of new tumors upon subsequent re-injection in cured mice. GBM patient-derived BVax were successful in activating autologous CD8+ T cells; these T cells showed a strong ability to kill autologous glioma cells. In addition to the role in activating CD8+ T cells, BVax produce tumor-specific antibodies able to control tumor growth via antibody-mediated cell cytotoxicity. In conclusion, BVax tackles GBM immunosurveillance escape by using both cellular (CD8+ T-cell activation) and humoral (anti-tumor antibody production) immunity. Our study provides an efficient alternative to current immunotherapeutic approaches that can be readily translated to the clinic.
MO‐C‐18A‐01: Advances in Model‐Based 3D Image Reconstruction (2014)

Recent years have seen the emergence of CT image reconstruction techniques that exploit physical models of the imaging system, photon statistics, and even the patient to achieve improved 3D image quality and/or reduction of radiation dose. With numerous advantages in comparison to conventional 3D filtered backprojection, such techniques bring a variety of challenges as well, including: a demanding computational load associated with sophisticated forward models and iterative optimization methods; nonlinearity and nonstationarity in image quality characteristics; a complex dependency on multiple free parameters; and the need to understand how best to incorporate prior information (including patient‐specific prior images) within the reconstruction process. The advantages, however, are even greater – for example: improved image quality; reduced dose; robustness to noise and artifacts; task‐specific reconstruction protocols; suitability to novel CT imaging platforms and noncircular orbits; and incorporation of known characteristics of the imager and patient that are conventionally discarded. This symposium features experts in 3D image reconstruction, image quality assessment, and the translation of such methods to emerging clinical applications. Dr. Chen will address novel methods for the incorporation of prior information in 3D and 4D CT reconstruction techniques. Dr. Pan will show recent advances in optimization‐based reconstruction that enable potential reduction of dose and sampling requirements. Dr. Stayman will describe a “task‐based imaging” approach that leverages models of the imaging system and patient in combination with a specification of the imaging task to optimize both the acquisition and reconstruction process. Dr. Samei will describe the development of methods for image quality assessment in such nonlinear reconstruction techniques and the use of these methods to characterize and optimize image quality and dose in a spectrum of clinical applications. Learning Objectives: Learn the general methodologies associated with model‐based 3D image reconstruction. Learn the potential advantages in image quality and dose associated with model‐based image reconstruction. Learn the challenges associated with computational load and image quality assessment for such reconstruction methods. Learn how imaging task can be incorporated as a means to drive optimal image acquisition and reconstruction techniques. Learn how model‐based reconstruction methods can incorporate prior information to improve image quality, ease sampling requirements, and reduce dose.