gebhart/patrepeval huggingface dataset:
Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells (2021)

Seasonal influenza epidemics occur both in northern and southern hemispheres every year. Despite the differences in influenza virus surface antigens and virulence of seasonal subtypes, manufacturers are well-adapted to respond to this periodical vaccine demand. Due to decades of influenza virus research, the development of new influenza vaccines is relatively straight forward. In similarity with the ongoing coronavirus disease 2019 pandemic, vaccine manufacturing is a major bottleneck for a rapid supply of the billions of doses required worldwide. In particular, egg-based vaccine production would be difficult to schedule and shortages of other egg-based vaccines with high demands also have to be anticipated. Cell culture-based production systems enable the manufacturing of large amounts of vaccines within a short time frame and expand significantly our options to respond to pandemics and emerging viral diseases. In this study, we present an integrated process for the production of inactivated influenza A virus vaccines based on a Madin-Darby Canine Kidney (MDCK) suspension cell line cultivated in a chemically defined medium. Very high titers of 3.6 log<sub>10</sub> (HAU/100 µl) were achieved using fast-growing MDCK cells at concentrations up to 9.5 × 10<sup>6</sup> cells/ml infected with influenza A/PR/8/34 H1N1 virus in 1 L stirred tank bioreactors. A combination of membrane-based steric-exclusion chromatography followed by pseudo-affinity chromatography with a sulfated cellulose membrane adsorber enabled full recovery for the virus capture step and up to 80% recovery for the virus polishing step. Purified virus particles showed a homogenous size distribution with a mean diameter of 80 nm. Based on a monovalent dose of 15 µg hemagglutinin (single-radial immunodiffusion assay), the level of total protein and host cell DNA was 58 µg and 10 ng, respectively. Furthermore, all process steps can be fully scaled up to industrial quantities for commercial manufacturing of either seasonal or pandemic influenza virus vaccines. Fast production of up to 300 vaccine doses per liter within 4-5 days makes this process competitive not only to other cell-based processes but to egg-based processes as well.
Modulation of Phosphoenolpyruvate Carboxylase Phosphorylation in Leaves of Amaranthus hypochondriacus, a NAD-ME Type of C<sub>4</sub> Plant (2000)

PEP carboxylase (PEPC) in leaves of C4 plants is activated by phosphorylation of enzyme by a PEPC-protein kinase (PEPC-PK). We reevaluated the pattern of PEPC phosphorylation in leaf extracts of Amaranthus hypochondriacus. It was dependent on Ca2+, the optimum concentration of which for stimulation was 10 mM. The extent of stimulation was inhibited by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), a Ca2+ chelator. The inhibition by BAPTA was relieved by the addition of Ca2+ but not by the addition of Mg2+. The stimulation by Ca2+ of PEPC phosphorylation was marginally enhanced by calmodulin (CaM), but not by diacylglycerol (DAG). Phosphorylation was strongly restricted by Ca2+ or Ca2+-CaM-dependent protein kinase inhibitors. Thus phosphorylation of PEPC is Ca2+-dependent in leaves of A. hypochondriacus and a calcium-dependent protein kinase (CDPK) may modulate PEPC-PK and subsequently the phosphorylation status of PEPC.
Influence of Hot Band Annealing on Cold-Rolled Microstructure and Recrystallization in AA 6016 (2022)

Abstract The influence of an intermediate heat treatment at the end of hot rolling and before cold rolling on Cube texture formation during the final solution annealing of AA 6016 is investigated. Three hot bands with different initial grain sizes and textures are considered: the first one without annealing before cold rolling, while the other two hot bands are heat treated at 540 °C for 1 hour in air before being cold rolled. One of the heat-treated hot bands was left to cool down in air and the other inside the furnace. Electron-backscatter diffraction (EBSD) maps of the cold-rolled specimens and crystal plasticity simulations show no difference in the amount of Cube remaining in the microstructure at the end of cold rolling for all three specimens. The initial grain size of the hot band has no influence on the Cube texture fraction left in the microstructure at the end of cold rolling for thickness reductions higher than 65 pct. Nevertheless, the grain size of the hot band affects the shape and distribution of the Cube grains left in the microstructure and the kernel average misorientation in the cold-rolled specimens. Moreover, the heat treatment decreases the intensity of the beta fiber components (Brass, Copper, and S) in the hot band and promotes the formation of a cold-rolled microstructure with a low kernel average misorientation. Both these factors lower the probability of preferential Cube nucleation during solution annealing and keep the Cube volume fraction after recrystallization below 10 pct, while it reaches 25 pct without intermediate annealing.
Lamotrigine in the treatment of resistant bipolar disorder. (1998)

Antiepileptic drugs, such as carbamazepine and sodium valproate, are integral to psychopharmacologic therapy over the last decades. Lamotrigine is a new antiepileptic drug that acts on amino acid neurotransmitters/neuromodulators, most prominently, glutamine, which has been reported to have potential mood-stabilizing effects. The reports on this effect are limited and sparse, and systematic investigations have not been published. Here we present two clinical cases representing atypical patient groups in which lamotrigine was successfully used as an add-on therapy for resistant bipolar depression.
New pharmacologic options in the treatment of acute coronary syndromes and myocardial ischemia-reperfusion injury: potential role of levosimendan. (2007)

Modern and effective therapeutic possibilities have improved the management and outcomes in acute coronary syndromes and acute myocardial infarction. However, substantial morbidity and mortality still remain. Myocardial ischemia-reperfusion injury may contribute to additional damage to myocardial necrosis and apoptosis. Therefore, it has been focused on attention and field of therapeutic actions in the last years. The main mechanisms involved in the pathogenesis of ischemia-reperfusion injury are depressed energy metabolism, elevated oxidative damage, and altered calcium homeostasis. In experimental trials, a variety of drugs have proved effectiveness for the prevention and treatment of the ischemia-reperfusion injury. However, its efficacy, not always confirmed, has not yet been established in clinical practice. On the basis of the strong evidence linking potassium ATP dependent channels opening in the myocardium and its proved cardioprotective role during ischemia, these channels have been pointed out as possible and promising pharmacological targets in this setting. Some evidences suggest that the calcium sensitizing agent levosimendan may have of beneficial and exerts cardioprotective effects on myocardial ischemia and ischemia-reperfusion injury. Further investigation is warranted on this novel application of levosimendan.
Einführung in die Systemtheorie (1997)

Das Werk einschließlich aller seiner Teile ist urheberrechtIich geschützt
<title>Direct writing of luminescent nanostructures in lithium fluoride films by x-ray microprobe scanning</title> (2005)

The continuously shrinking of the photonic structures has stimulated the research and development of low-dimensionality photoluminescent patterns based on visible-emitting color centers (CCs) in thin films. In particular, Lithium Fluoride (LiF) treated with ionizing radiation by using versatile lithographic techniques, represents a very promising candidate for the realization of innovative photonic devices. Permanent tluorescent patterns based on CCs have been realized in LiF films by direct writing with a X ray microprobe -of the ESCA Microscopy beamline at ELETTRA synchrotron in different configuration. Luminescent nanostructures, written by scanning the LW specimen with respect to the X-ray beam, have been investigated by near-field optical microscope and a sub-wavelength spatial resolution is demonstrated.
Experimental vaccination of young chickens with a live, non‐pathogenic strain ofEscherichia coli (1994)

A non-pathogenic, piliated strain of Escherichia coli (BT-7; Frommer et al., 1990), isolated from a meat-type chicken flock, was studied as a candidate for a live vaccine to protect chickens from E. coli infection. Active immunization provided substantial protection of chicks vaccinated at 14 or 21 days of age, resulting in better resistance to challenge than in those vaccinated at 1 or 7 days. Chicks vaccinated at 21 days of age and challenged 1 week later with pathogenic E. coli strains 01-.K1, 02:K1 or 078:K80, exhibited good protection for at least 2 weeks against all strains. Three vaccination routes were found to give the highest resistance to challenge with pathogenic E. coli strain 078:K80. Intramuscular (i.m.) at 7 and 21 days of age, i.m. at 21 days of age and spray at 7, followed by per os at 21 days of age. Vaccination per os once at 7 or twice at 7 and 21 days resulted in good protection. Chicks exhibiting high antibody titres by ELISA were well-protected against challenge.
Pedagogy of Tarot: simultaneity of past, present, and future (2020)

A three card tarot spread can represent the past, present, and future. As a reflective practice, tarot does not divine the future; rather it invites the practitioner to consider context and imagine multiple futures. Simultaneously experiencing the past, present, and future of education is valuable and is possible through a pedagogy of tarot. A pedagogy of tarot connects fxminist and democratic approaches to education through non-hierarchical relationships that honor lived experiences - calling teachers and learners to remain conscious and awake to one another. By acknowledging the possibility of multiple truths within current sociopoliticial and hxstorical contexts, we can make space for multiple paths forward.
New technologies for site selection and evaluation of salt-cavern underground gas storages (2020)

In order to meet the demand of constructing salt-cavern underground gas storages (UGSs) in favorable salt mines for the regions that are neither major oil and gas production zones nor major natural gas consumer markets in South China, and solve the problems of unclear conditions for selecting salt-cavern gas storage sites and of inconsistent content and depth in evaluating gas storage conditions, based on investigation and analysis of salt-cavern UGS data in China and other countries, we proposed the principles of selecting gas storage sites in combination with geology and ground surface conditions, and developed a set of technologies in evaluating construction conditions for salt-cavern gas storages. By applying the selection principles of salt-cavern UGSs, we selected and sorted some salt mines in the three major natural gas consumer markets (the Yangtze River Delta, Central–South China, and Southeast Coast of China), and evaluated the conditions of constructing a gas storage in the Huai'an salt mine with better conditions. The following findings were obtained. First, the principles of selecting gas storage sites can be applied to quickly optimize and sort the selected targets, including 6 items of geological factors such as structure, burial depth, salt-bearing layer thickness, NaCl content, top rock, and reserves, and 3 other items such as ground surface conditions, water source and brine, distance from pipeline networks. Second, the evaluation system of UGS construction conditions integrates features of structure and salt-bearing strata, tightness, firmness, dissolubility evaluation, prediction of gas storage scale, and analysis of cavity making condition. In conclusion, the new evaluation technologies can not only effectively guide the site selection and evaluation of salt-cavern UGSs but provide foundation and power support for their scheme designs, and the presented pilot proposal for utilizing thick interlayers has achieved a perfect on-site application effect. This study is of great significance for constructing UGSs in China.
Preparation and characterization of superionic conducting Li7P3S11 crystal from glassy liquids (2010)

Superionic Li+ ion conducting Li7P3S11 crystal was prepared by crystallization of the 70Li2S·30P2S5 (mol %) glass and melt. In the case of crystallization of the 70Li2S·30P2S5 glass, the crystallized electrolyte heated at 360°C for 1 h showed a conductivity of 4.1 × 10−3 S cm−1 at room temperature. In the case of crystallization from the 70Li2S·30P2S5 melt, a single phase of the Li7P3S11 crystal was obtained by crystallization of the melt at 700°C for 48 h and then quenching in ice water. The crystallized electrolyte showed the conductivity of 2.9 × 10−3 S cm−1 at room temperature. The precipitated crystal depended on the heat treatment temperature, holding time, and cooling rate. In both crystallization processes from the glass and melt, the Li7P3S11 crystal was precipitated as a primary phase from supercooled liquids and thus the superionic conducting Li7P3S11 crystal is a high temperature phase at the composition 70Li2S·30P2S5.
HST-LSTM: A Hierarchical Spatial-Temporal Long-Short Term Memory Network for Location Prediction (2018)

The widely use of positioning technology has made mining the movements of people feasible and plenty of trajectory data have been accumulated. How to efficiently leverage these data for location prediction has become an increasingly popular research topic as it is fundamental to location-based services (LBS). The existing methods often focus either on long time (days or months) visit prediction (i.e., the recommendation of point of interest) or on real time location prediction (i.e., trajectory prediction). In this paper, we are interested in the location prediction problem in a weak real time condition and aim to predict users' movement in next minutes or hours. We propose a Spatial-Temporal Long-Short Term Memory (ST-LSTM) model which naturally combines spatial-temporal influence into LSTM to mitigate the problem of data sparsity. Further, we employ a hierarchical extension of the proposed ST-LSTM (HST-LSTM) in an encoder-decoder manner which models the contextual historic visit information in order to boost the prediction performance. The proposed HST-LSTM is evaluated on a real world trajectory data set and the experimental results demonstrate the effectiveness of the proposed model.
ChemInform Abstract: STRUCTURAL CRITERIA FOR HYDRAZONE PHOTOCHROMISM IN SOLUTION (1976)

Abstract Bei Bestrahlung der nach üblichen Methoden erhaltenen Salicylaldehydchinolylhydrazone (I) in Äthanol bei Zimmertemperatur entstehen die (Z)‐Benzylidenhydrazonochinoline (II) als farbige Form.
ChemInform Abstract: Briarenolides H and I: New 8‐Hydroxybriarane Diterpenoids from a Formosan Octocoral Briareum sp. (Briareidae). (2013)

Abstract Two new 8‐hydroxybriarane diterpenoids, briarenolide H (I) and I (II), are isolated.
Starch Digestibility as Affected by Polyphenols and Phytic Acid (1984)

ABSTRACT The rate of wheat starch digestibility in the presence or absence of polyphenols (catechin or tannic acid) and/or phytic acid at concentrations found in legumes was determined in an in vitro dialysis system. Addition of tannic acid and phytic acid reduced the starch digestibility 13 and 60% respectively, at 5 hr. Combined tannic and phytic acid reduced the digestibility at a level (63%) which did not differ significantly from that with only phytic acid. Catechin had no significant effect on rate of starch digestibility.
Bulk Growth of SiC – Review on Advances of SiC Vapor Growth for Improved Doping and Systematic Study on Dislocation Evolution (2009)

Summary

This chapter contains sections titled: 



Introduction




Experiments




Results and discussions




Spatial distribution of dislocations in SiC




Conclusions




References
Cell Membrane Oscillations under Radiofrequency Electromagnetic Modulation (2023)

Cell responses to external radiofrequencies (RF) are a fundamental problem of much scientific research, clinical applications, and even daily lives surrounded by wireless communication hardware. In this work, we report an unexpected observation that the cell membrane can oscillate at the nanometer scale in phase with the external RF radiation from kHz to GHz. By analyzing the oscillation modes, we reveal the mechanism behind the membrane oscillation resonance, membrane blebbing, the resulting cell death, and the selectivity of plasma-based cancer treatment based on the difference in the membrane's natural frequencies among cell lines. Therefore, a selectivity of treatment can be achieved by aiming at the natural frequency of the target cell line to focus the membrane damage on the cancer cells and avoid normal tissues nearby. This gives a promising cancer therapy that is especially effective in the mixing lesion of the cancer cells and normal cells such as glioblastoma where surgical removal is not applicable. Along with these new phenomena, this work provides a general understanding of the cell coupling with RF radiation from the externally stimulated membrane behavior to the cell apoptosis and necrosis.
Object Detection and Geometric Profiling through Dirty Water Media Using Asymmetry Properties of Backscattered Signals (2018)

The scattering of light observed through the turbid underwater channel is often regarded as the leading challenge when designing underwater electro-optical imaging systems. There have been many approaches to address the effects of scattering such as using pulsed laser sources to reject scattered light temporally, or using intensity modulated waveforms and matched filters to remove the scattered light spectrally. In this paper, a new method is proposed which primarily uses the backscattering asymmetry property for object detection and geometric profiling. In our approach, two parallel and identical continuous wave (CW) laser beams with narrow beam widths (~2mm) are used as active illumination sources. The two beams also have controllable spacing and aiming angle, as well as initial phase difference for convenience of scanning and profiling a target. Through theory and experimental results, it will be shown that when an object leans or tilts towards one of the beam's central trajectory, the asymmetry in the backscattered signals can be used to indicate the location or slope of the target's surface, respectively. By varying the spacing or aiming angle of the two beams, a number of surface samples can be collected to reconstruct the object's shape geometrically. The resolution and range limit of our approach are also measured and reported in this work. In application, our proposed method provides an economic solution to perform imaging through turbid underwater environments. Additionally, the idea can be combined with the pulsed or modulated laser signals for enhanced imaging results.
Programming 1 and sustainability (2022)

Computer programming is an essential skill for today's engineers, and sustainability plays a role of growing interest in any of the design phases of an engineering project. The fundamentals of sustainability, with basic concepts such as "carbon intensity", must be covered in any engineering curriculum. We propose a basic computer programming course in which the lab sessions incorporate exercises related to the computation of environmental impacts. For instance, an exercise might request the computation of the carbon footprint of the lab sessions during a whole year. Lab sessions use an automatic evaluation server, so called Jutge.org that assesses about the correctness of the programs submitted by the students. New exercises concerning sustainability topics are included in the Jutge.org course. The course involves the effort of a multidisciplinary team. First, lecturers on basic programming are required. An expert on automatic evaluation of computer programs is essential to prepare the statements and the test sets of the proposed exercises. Finally, the advice of economists and sustainability experts is crucial to guarantee judicious conclusions are drawn from each exercise. Forming a team with this profile is a challenging task. Our Computer Science (CS) department lectures basic programming courses to more than 1700 students/year. The success of this approach could bring a substantial social impact in our ecosystem.
Intentional Replantation to Treat Apical Periodontitis of Maxillary First Molar with Foreign Body Located Outside Apical Foramen Using CBCT: A Case Report (2020)

The recent use of cone beam computed tomography (CBCT) in the field of dentistry to obtain 3-dimensional (3D) images has enabled more effective examination and diagnosis in endodontic treatment. Such information has also been reported to be of benefit in surgical endodontic treatment such as intentional replantation. Here, we report a case of intentional replantation with the assistance of CBCT in which a good therapeutic outcome was achieved. The patient was a 30-year-old woman who visited our hospital with the chief complaint of spontaneous pain in the right maxillary first molar. Dental radiographs revealed a radiolucent area in the apical portion of the distal root, and a radiopaque area thought to be a broken shard from a small surgical instrument measuring approximately 1.5 mm in length. The shard was located outside the distal apical foramen. Based on these findings, the diagnosis was acute suppurative apical periodontitis of the right maxillary first molar. Infected root canal therapy was subsequently commenced. The patient's symptoms showed no improvement, however, and the pain persisted. Therefore, dental CBCT was performed to obtain 3D images, which confirmed a radiopaque area thought to be a broken shard from a small surgical instrument located outside the apical foramen of the distal root and facing in a direction that made it impossible to remove from the root canal. Because the patient's symptoms had shown no improvement and a foreign body was observed outside the apical foramen, intentional replantation combined with root resection was performed with informed consent. At a 1-year follow-up visit, progress was good, and there were no reported symptoms or signs. When reaching a diagnosis is difficult based on clinical findings and dental radiographs alone, the 3D images provided by CBCT offer a means of securing a more reliable diagnosis, allowing planning of treatment to be more effective.