gebhart/patrepeval huggingface dataset:
Development of a Modular Cadaveric Endoscopic Orbital Surgery Model (2019)

Introduction Endoscopic orbital surgery requires the acquisition of unique skill set including endoscopic bimanual dissection of intra/extraconal lesions adherent to orbital fat and neurovascular structures. Our goal was to develop a modular cadaveric model used to train surgeons to resect orbital pathology within any desired orbital compartment. Methods Expansile superabsorbent polymer (SAP) beads (2 mm) were soaked in Omnipaque™ (Iohexol) solution for 15 minutes prior to transcaruncular orbital implantation using 10-gauge angiocath. Insertion depth was designed to implant beads in predetermined intraconal compartments corresponding to established orbital tumor stages. Beads were left to expand in situ over a period of 1 to 5 hours. Computed tomography scans were performed using the FUSION image guidance protocol. Model utility and learning curves were assessed by quantifying resection time over 8 sequential attempts. Results All 24 beads were successfully implanted in 8 orbits corresponding to CHEER stage II to IV lesions (n = 3 per orbit). Beads expanded from 2 mm to an average of 5.2 mm within 1 hour. During expansion, the beads interpolated into the adjacent fascia similar to in vivo tumors. Average insertion time was 5:53 minutes per orbit (range, 3:24–10:33 min) and average time to bead identification was 10:47 minutes. Across all beads, dissection times decreased in a nonsignificant manner over 8 consecutive attempts. Conclusion The directed implantation of expansile SAP beads in this cadaveric model accurately replicates the approach, identification, and resection of isolated orbital lesions. This orbital model can assist the endoscopic surgical team to develop further knowledge and technical skill sets to approach orbital lesions. Further ongoing studies to validate this model are currently underway.
A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes (2006)

An ultrasensitive sandwich DNA array using highly fluorescent and photostable dyedoped silica nanoparticles is described. Compared to traditional sandwich arrays in which fluorophores have been used to signal target DNA molecules, the developed nanoparticle probes provide a much stronger fluorescent emission. Signal amplification of the dyedoped silica nanoparticles originates from the large number of dye molecules doped inside each individual nanoparticle. In addition, the silica matrix of the nanoparticles protects dye molecules from photobleaching. Thus, the dye-doped nanoparticles provide a constant fluorescent signal that is sufficient for detection of trace amounts of target DNA. By immobilizing a complementary DNA sequence to the target onto the nanoparticle surface, a fluorescent nanoparticle-DNA probe is formed. These nanoparticle probes are then used as superemitting reagents to perform a typical sandwich assay. By using a high-resolution fluorescent microscope, individual nanoparticle-DNA probes that have been hybridized to capture target strands can be observed clearly at low target DNA concentrations. More important, the number of the nanoparticle-DNA probes hybridized to the target DNA is proportional to the target DNA concentration in solution. By counting the number of localized fluorescent "spots" on the array, the target DNA concentration can be determined. In this chapter, detailed methods used to synthesize nanoparticle-DNA probes, fabricate the sandwich array, prepare the substrate, and quantitatively determine DNA concentration are described.
Detection of the fragile X chromosome and other fragile sites (1984)

To assist in cell sample size selection and detect the fragile X chromosome, statistical tables have been presented. A comparable approach had been suggested earlier for the diagnosis of chromosome mosaicism. The cytologic detection of the fragile X or any fragile site is simply a special case in the detection of mosaicism. Minimum numbers of metaphases are recommended for fragile X analysis to have 95% confidence that the fragile X is not manifest in a given proportion of cells and similar recommendations apply to all other fragile sites.
Performance of a new magnetorheological elastomer isolation system (2014)

This paper presents the performance of a new magnetorheological elastomer-based semi-active/passive variable stiffness and damping isolator (VSDI) in a scaled building system. The force of the VSDI can be controlled in real time by varying the applied magnetic field. To demonstrate the performance of the VSDI, four prototypes are built and utilized in a scaled three-story building. A Lyapunov-based control strategy is employed and it is demonstrated that it works well for the scaled building system under the scaled El Centro earthquake motion. Experimental results show that the VSDIs significantly reduce the acceleration and relative displacement of the building floors.
Emerging roles of extracellular vesicles in cardiac repair and rejuvenation (2018)

Cell therapy has received significant attention as a therapeutic approach to restore cardiac function after myocardial infarction. Accumulating evidence supports that beneficial effects observed with cell therapy are due to paracrine secretion of multiple factors from transplanted cells, which alter the tissue microenvironment and orchestrate cardiac repair processes. Of these paracrine factors, extracellular vesicles (EVs) have emerged as a key effector of cell therapy. EVs regulate cellular function through the transfer of cargo, such as microRNAs and proteins, which act on multiple biological pathways within recipient cells. These discoveries have led to the development of cell-free therapies using EVs to improve cardiac repair after a myocardial infarction. Here, we present an overview of the current use of EVs to enhance cardiac repair after myocardial infarction. We also discuss the emerging use of EVs for rejuvenation-based therapies. Finally, future directions for the use of EVs as therapeutic agents for cardiac regenerative medicine are also discussed.
Cocktail watermarking for digital image protection (2000)

A novel image protection scheme called "cocktail watermarking" is proposed in this paper. We analyze and point out the inadequacy of the modulation techniques commonly used in ordinary spread spectrum watermarking methods and the visual model-based ones. To resolve the inadequacy, two watermarks which play complementary roles are simultaneously embedded into a host image. We also conduct a statistical analysis to derive the lower bound of the worst likelihood that the better watermark (out of the two) can be extracted. With this "high" lower bound, it is ensured that a "better" extracted watermark is always obtained. From extensive experiments, results indicate that our cocktail watermarking scheme is remarkably effective in resisting various attacks, including combined ones.
Opportunities and challenges of the 450mm transition (2013)

A close collaboration among IC makers, equipment and material suppliers, facility, and automation providers resulted in better alignment in the technology roadmap and lower development costs for tools and wafers. This would enable the cost-effective operation of future 450mm HVM fabs. The 450mm transition will certainly create another prosperous opportunity for the semiconductor industry.
Insights into Molecular Structures and Optical Properties of Stacked [Au3(RN═CR′)3]n Complexes (2017)

The molecular structure of stacked cyclic trinuclear gold(I) complexes [Au<sub>3</sub>(RN═CR')<sub>3</sub>]<sub>n</sub>, with n = 1-4, where R = H, methyl (Me), cyclopentyl (<sup>c</sup>Pe), and phenyl (Ph) and R' = OH and methoxy (OMe) were studied computationally at the second-order Møller-Plesset (MP2) and density functional theory (DFT) levels of theory. At the DFT level, the aurophilic and dispersion interactions were accounted for by using the TPSS functional in combination with the semiempirical D3 correction. The structure optimizations yielded the lowest energy for a slided stacked structure of the [Au<sub>3</sub>(HN═COH)<sub>3</sub>]<sub>2</sub> dimer, where monomers are slightly shifted relative to one another. At the MP2 level, the slided structure is 32 kJ/mol more stable than the staggered dimer structure, which in turn is energetically 11 kJ/mol below the eclipsed structure. The calculations show that aromatic ligands lead to a planar and prismatic structure of [Au<sub>3</sub>(PhN═COMe)<sub>3</sub>]<sub>4</sub>, whereas for [Au<sub>3</sub>(<sup>c</sup>PeN═COMe)<sub>3</sub>]<sub>4</sub>, a chair conformation is obtained due to steric effects. Excitation energies were calculated for [Au<sub>3</sub>(RN═CR')<sub>3</sub>] and [Au<sub>3</sub>(RN═CR')<sub>3</sub>]<sub>2</sub> with R = H, Me, and <sup>c</sup>Pe and R' = OH and OMe at the time-dependent DFT level using the optimized molecular structures of the singlet ground state. To simulate the luminescence spectra, the lowest triplet excitation energy was also calculated for the molecular structure of the lowest triplet state. The calculated excitation energies of [Au<sub>3</sub>(HN═COH)<sub>3</sub>] and [Au<sub>3</sub>(HN═COH)<sub>3</sub>]<sub>2</sub> are compared with values obtained at the approximate singles and doubles coupled cluster (CC2) and the second-order algebraic diagrammatic construction (ADC(2)) levels of theory. The calculated absorption and emission energies reproduce the experimental trends, with extremely large Stokes shifts. A solvoluminescence mechanism is also proposed.
Transcriptome analysis of well-differentiated laryngeal squamous cell carcinoma cells in below-background environment (2022)

Below-background radiation can lead to transcriptional changes in FD-LSC-1 cells cultured in the deep underground. The inhibitory growth effect is associated with multiple biological processes as well as canonical pathways of proliferation.
Polymorphism in Solid Dispersions (2019)

Solid dispersions embed active pharmaceutical ingredients in polymeric carriers to improve their solubility. Three solid dispersion preparation techniques are typically employed: solvent evaporation, solvent-fusion, and fusion methods. Although these are also widely recommended as preparative methods for phase diagram determination, few examples exist concerning their effect on the resulting polymorph, once the solid dispersion is produced. In this study, the influence of these methods on the polymorphic form obtained in crystalline solid dispersions (CSDs) composed of flufenamic acid (FFA) and poly(ethylene glycol) was investigated. The physical mixtures and CSDs were characterized by powder X-ray diffraction, infrared spectroscopy, and differential scanning calorimetry. The results reveal that the fusion method leads to concomitant polymorphs (mainly FFA I and III) in the CSDs. In contrast, the solvent evaporation and solvent-fusion methods lead to FFA III. Collectively, these results demonstrate that preparative methods have a significant influence on the phase diagrams determined (average relative deviation ≤8%), which are often used to justify the design space of manufacturing processes, including those deemed "continuous." Consequently, choosing a preparation method that results in the desired polymorph is crucial to ensure accurate determination of phase diagrams and critical quality attributes of formulations.
The EDN1 Missense Variant rs5370G > T Regulates Adaptation and Maladaptation under Hypobaric Hypoxia (2022)

Endothelin 1 (<i>EDN1</i>) encodes a potent endogenous vasoconstrictor, ET1, to maintain vascular homeostasis and redistribution of tissue blood flow during exercise. One of the <i>EDN1</i> missense polymorphisms, rs5370 <i>G/T</i>, has strongly been associated with cardiopulmonary diseases. This study investigated the impact of rs5370 polymorphism in high-altitude pulmonary oedema (HAPE) disorder or maladaptation and adaptation physiology in a well-characterized case-control study of high-altitude and low-altitude populations comprising 310 samples each of HAPE-patients, HAPE-free controls and native highlanders. The rs5370 polymorphism was genotyped, and the gene expression and plasma level of EDN1 were evaluated. The functional relevance of each allele was investigated in the human embryonic kidney 293 cell line after exposure to hypoxia and computationally. The T allele was significantly more prevalent in HAPE-p compared to HAPE-f and HLs. The <i>EDN1</i> gene expression and ET1 bio-level were significantly elevated in HAPE-p compared to controls. Compared to the G allele, the T allele was significantly associated with elevated levels of ET-1 in all three study groups and cells exposed to hypoxia. The <i>in silico</i> studies further confirmed the stabilizing effect of the T allele on the structural integrity and function of ET1 protein. The ET1 rs5370 T allele is associated with an increased concentration of ET-1 in vivo and in vitro, establishing it as a potent marker in the adaptation/maladaptation physiology under the high-altitude environment. This could also be pertinent in endurance exercises at high altitudes.
Research “Values” in the Humanities: Funding Policies, Evaluation, and Cultural Resources. Some Introductory Remarks (2015)

In her capacity as guest editor, the author introduces a set of essays examining the trends, risks, needs, pressures, and prospects of the humanities after recent reforms to tertiary education throughout Europe. By focusing on the educational, cultural, and social value of research in the humanities, which also provide economic and democratic benefits, this special issue focuses on three key topics: “funding policies”, “evaluation”, and “cultural resources”. This article provides the background to the subject matter (Section 1); a reflection on the controversial issues of quality control, measures of research productivity, and funding decisions as key drivers changing the humanities (Section 2); an overview of the current difficulties and prospects for “modernizing” the humanities (Section 3); the rationale for this special issue (Section 4); the context and a synopsis of the contributions, showing how and why these position papers by members of the humanities cluster of the Academia Europaea can provide this debate with new tools of analysis and diagnosis (Section 5). Finally, the concluding remarks highlight the Academia Europaea’s actions for the humanities (Section 6).
Parametric and non-parametric models of short-term plasticity (2002)

Short-term plasticity (STP) was recorded with voltage-clamp technique and modeled with both parametric and nonparametric approaches. In the parametric approach, we simulated STP by calculating facilitation and refractory depression driven by presynaptic residual calcium. In the nonparametric approach, STP was modeled using Laguerre expansions of Volterra kernels. Our results showed that the 3/sup rd/ order non-parametric model sufficiently captured the nonlinearities of the parametric model.
Dose-related effects of epinephrine on glucose production in conscious dogs (1991)

The effects of increases in plasma epinephrine from 78 +/- 32 to 447 +/- 75, 1,812 +/- 97, or 2,495 +/- 427 pg/ml on glucose production, including gluconeogenesis, were determined in the conscious, overnight-fasted dog, using a combination of tracer [( 3-3H]glucose and [U-14C]alanine) and arteriovenous difference techniques. Insulin and glucagon were fixed at basal levels using a pancreatic clamp. Plasma glucose levels rose during the 180-min epinephrine infusion by 47 +/- 7, 42 +/- 22, and 74 +/- 25 mg/dl, respectively, in association with increases in hepatic glucose output of 1.04 +/- 0.22, 1.87 +/- 0.23, and 3.70 +/- 0.83 mg.kg-1.min-1 (at 15 min). Blood lactate levels rose by 1.52 +/- 0.24, 4.29 +/- 0.49, and 4.60 +/- 0.45 mmol/l, respectively, by 180 min, despite increases in hepatic uptake of lactate of 3.47 +/- 5.73, 12.83 +/- 3.46, and 37.00 +/- 4.20 mumol.kg-1.min-1. The intrahepatic gluconeogenic efficiency with which the liver converted the incoming alanine to glucose had risen by 84 +/- 40, 77 +/- 24, and 136 +/- 34% at 180 min, respectively. The latter effect plus the effect on net hepatic lactate uptake point to an intrahepatic action of high levels of the hormone in vivo. In conclusion, epinephrine produces dose-dependent increments in overall glucose production, which involve a progressive stimulation of both glycogenolysis (as assessed by glucose production at 15 min) and gluconeogenesis (assessed in the last 30 min of the study). The latter involves a peripheral action of the catecholamine to increase gluconeogenic substrate supply to the liver and may also involve a hepatic effect when high epinephrine levels are present.
ChemInform Abstract: METHOD FOR THE SYNTHESIS O‐CARBOXYMETHYLHYDROXYLAMINE HYDROCHLORIDE (1982)

Abstract Aceton‐oxim (I) reagiert mit dem Na‐Salz von Chloressigsäure zum Derivat (III), dessen Hydrolyse die im Titel genannte Verbindung (IV) liefert.
Importance of Sample Preparation for Molecular Diagnosis of Lyme Borreliosis from Urine (2002)

Urine PCR has been used for the diagnosis of Borrelia burgdorferi infection in recent years but has been abandoned because of its low sensitivity and the irreproducibility of the results. Our study aimed to analyze technical details related to sample preparation and detection methods. Crucial for a successful urine PCR were (i) avoidance of the first morning urine sample; (ii) centrifugation at 36,000 x g; and (iii) the extraction method, with only DNAzol of the seven different extraction methods used yielding positive results with patient urine specimens. Furthermore, storage of frozen urine samples at -80 degrees C reduced the sensitivity of a positive urine PCR result obtained with samples from 72 untreated erythema migrans (EM) patients from 85% in the first 3 months to <30% after more than 3 months. Bands were detected at 276 bp on ethidium bromide-stained agarose gels after amplification by a nested PCR. The specificity of bands for 32 of 33 samples was proven by hybridization with a GEN-ETI-K-DEIA kit and for a 10 further positive amplicons by sequencing. By using all of these steps to optimize the urine PCR technique, B. burgdorferi infection could be diagnosed by using urine samples from EM patients with a sensitivity (85%) substantially better than that of serological methods (50%). This improved method could be of future importance as an additional laboratory technique for the diagnosis of unclear, unrecognized borrelia infections and diseases possibly related to Lyme borreliosis.
136 MRI MICROSCOPY OF THE DEVELOPING CHICK EMBRYO (1986)

Department of Radiology, Duke Medical Center, Durham, NC National Institute of Environmental Health Science, Research Triangle Park, NC
The pharmacological management of hypertension in pregnancy (1992)

Decisions regarding the need for antihypertensive treatment during pregnancy and the selection of a specific antihypertensive agent should be based upon an assessment of the relative benefits and risks for the individual patient. In future studies, the effects of antihypertensive agents upon the underlying pathophysiological processes involved in PIH/preeclampsia may guide therapeutic decision making.
Storage of buffy-coat-derived platelets in additive solution: in vitro effects on platelets of the air bubbles and foam included in the final unit. (2011)

This study shows that storage with air bubbles/foam causes considerable enhancement of disintegration of platelets. In addition, various in vitro parameters of the platelets remaining seem to be negatively affected. The results of this study suggest that platelets should be stored without air bubbles/foam, given that these cause increased disintegration of platelets.
Drying characteristics of starch in an inert medium fluidized bed (1993)

Abstract The effects of gas velocity (0.32 to 0.67 m/s), inlet gas temperature (25 to 100 °C) and the mass ratio of starch to inert particles (0.1 to 0.4) on the drying rate of starch in a 0.083 m‐ID × 0.80 m‐high inert medium fluidized bed were investigated. The drying mechanism in an inert medium fluidized bed can be classified into adhesion‐dispersion, evaporation and disintegration steps. The drying rate increases with the increasing inlet gas temperature and velocity; the rates being about 10 times those reported for an agitated pan dryer. However, the drying rate decreases slightly as the mass ratio of starch to inert particles increases. Also, the drying rate exhibits a maximum at an optimum bed porosity. The drying rate data obtained in an inert medium fluidized bed have been correlated with the relevant dimensionless groups, i.e. Stefan and particle Reynolds numbers based on the theory of isotropic turbulence.
Big Data for Sensing (2017)

This chapter considers a sensing system as a big data system and models the massive amount of data with the aid of large random matrices. An emerging field is the use of wireless sensor networks (WSNs) as a support for smart grids. In such a case, a WSN is useful to: monitor and predict energy production from renewable sources of energy such as wind or solar energy, monitor energy consumption, and detect anomalies in the network. The proper way to describe the interactions among the network nodes is to introduce the graph model of the network. Euclidean random matrices play an important role in description of many physical models including the electronic levels in a morphous systems, very diluted impurities, and the spectrum of vibrations in glasses. The chapter talks about the connection between, the Euclidean random matrix and the massive MIMO (or WSN).
TOPOLOGY AND DISTINCT FEATURES OF FLASHING FLOW IN AN INJECTOR NOZZLE (2016)

The effect of thermodynamic non-equilibrium conditions (liquid superheat) on the two-phase flow field developing inside an axisymmetric, single-orifice nozzle is numerically investigated by means of different variations of a two-phase mixture model. A number of "hybrid" mass-transfer models that take into account both the effect of inertial forces (cavitation) and liquid superheat have been proposed and evaluated against widely used, pure-cavitation models, in order to pinpoint the flow conditions necessary for flash boiling to occur and to elucidate the distinct features of the phase and velocity fields that characterize flashing flows. The effect of the number of nucleation sites, required as an input by the models, on the developing two-phase flow has also been looked into. The numerical results have shown that incorporation of an additional term corresponding to liquid superheat into the mass-transfer rate leads to increased evaporation rate, compared to pure-cavitation models with liquid vaporization taking place within the entire nozzle cross section. The cavitation nucleation sites have been confirmed to act as the necessary flow perturbations required for flash boiling to occur. In addition, the developing velocity field has been found to be in close correlation to the mass-transfer rate imposed. It has been established that increased liquid evaporation leads to choked-flow conditions prevailing in a larger part of the nozzle and accompanied by a more significant expansion of the two-phase mixture downstream of the injector exit that results to increased jet cone angle. Finally, the results demonstrated that liquid cooling due to the increased mass-transfer rate is not significant within the nozzle and thus consider that a constant liquid temperature produces adequately accurate results with a decreased computational cost.