gebhart/patrepeval huggingface dataset:
Post-transplant outcome of ovarian tissue cryopreserved after chemotherapy in hematologic malignancies (2019)

International audience
Upscaling Effects on Alkali Metal‐Grafted Ultrastable Y Zeolite Extrudates for Modeled Catalytic Deoxygenation of Bio‐oils (2021)

Abstract Developing efficient solid catalysts is necessary when for example moving from batch chemistry to continuous flow systems. In this work, scale‐up effects of zeolite‐based catalyst materials have been tested in aldol condensation as a model reaction for bio‐oil upgrading via deoxygenation. For this purpose, shaped catalyst bodies were obtained via extrusion of ultrastable Y zeolite (USY) using either attapulgite (Att.) or bentonite (Bent.) as clay binder, followed by post‐alkali metal ion grafting of K + after (rather than before) extrusion. This approach proved essential to preserve the catalysts’ crystallinity. The Att.‐bound catalyst body was more active than its Bent.‐counterpart, correlating well with the observed changes in physicochemical properties. The K‐(USY/Att.) catalyst showed new basic oxygen and strong Lewis acidic sites resulting from clay incorporation, in addition to the Lewis acid (K + ) and basic sites (K−OH) created upon grafting. For K‐(USY/Bent.), the grafting proved less efficient, likely due to pore blockage caused by the binder. Bent. addition resulted in acid sites of moderate Brønsted and strong Lewis acidity, but hardly any of the basicity desired for the aldol condensation reaction. The poor potassium grafting yet led to some cation exchange with the binder (likely with the Na + naturally present in the Bent. material). The results obtained demonstrate the critical importance of the choice of the binder material and the synthesis protocol adopted for upscaling solid base materials in the form of catalyst bodies.
Effect of racemic ibuprofen dose on the magnitude and duration of platelet cyclo‐oxygenase inhibition: relationship between inhibition of thromboxane production and the plasma unbound concentration of S(+)‐ ibuprofen. (1991)

1. Four healthy male subjects received racemic ibuprofen (200, 400, 800 and 1200 mg), orally, on four occasions, 2 weeks apart, according to a four-way Latin-square design, in order to investigate the influence of increasing dose of ibuprofen on the magnitude and duration of its antiplatelet effect as well as on the relationship between such effect and drug concentration. 2. The antiplatelet effect of ibuprofen was assessed by measuring the inhibition of platelet thromboxane B2 (TXB2) generation during the controlled clotting of whole blood. The plasma unbound concentration of S(+)-ibuprofen, the enantiomer shown in an in vitro study to be responsible for the inhibitory effect of platelet TXB2 generation, was measured using an enantioselective method. 3. The maximum percentage inhibition of TXB2 generation increased significantly with dose from a mean +/- s.d. of 93.4 +/- 1.2% after the 200 mg dose to 98.8 +/- 0.3% after the 1200 mg dose, and there was an increase with dose in the duration of inhibition of TXB2 generation. The effect of ibuprofen on platelet TXB2 generation was transient and mirrored the time-course of unbound S(+)-ibuprofen in plasma; on all but one of the 16 occasions, serum TXB2 concentrations returned to at least within 10% of the pretreatment concentrations within 24 h of ibuprofen administration. 4. For each subject, the relationship between the percentage inhibition of TXB2 generation and the unbound concentration of S(+)-ibuprofen in plasma was modelled according to a sigmoidal Emax equation. The mean plasma unbound concentration of S(+)-ibuprofen required to inhibit platelet TXB2 generation by 50% (EC50) was 9.8 +/- 1.0 micrograms l-1.(ABSTRACT TRUNCATED AT 250 WORDS)
Chromosomal Rearrangement Inferred From Comparisons of 12 Drosophila Genomes (2008)

The availability of 12 complete genomes of various species of genus Drosophila provides a unique opportunity to analyze genome-scale chromosomal rearrangements among a group of closely related species. This article reports on the comparison of gene order between these 12 species and on the fixed rearrangement events that disrupt gene order. Three major themes are addressed: the conservation of syntenic blocks across species, the disruption of syntenic blocks (via chromosomal inversion events) and its relationship to the phylogenetic distribution of these species, and the rate of rearrangement events over evolutionary time. Comparison of syntenic blocks across this large genomic data set confirms that genetic elements are largely (95%) localized to the same Muller element across genus Drosophila species and paracentric inversions serve as the dominant mechanism for shuffling the order of genes along a chromosome. Gene-order scrambling between species is in accordance with the estimated evolutionary distances between them and we find it to approximate a linear process over time (linear to exponential with alternate divergence time estimates). We find the distribution of synteny segment sizes to be biased by a large number of small segments with comparatively fewer large segments. Our results provide estimated chromosomal evolution rates across this set of species on the basis of whole-genome synteny analysis, which are found to be higher than those previously reported. Identification of conserved syntenic blocks across these genomes suggests a large number of conserved blocks with varying levels of embryonic expression correlation in Drosophila melanogaster. On the other hand, an analysis of the disruption of syntenic blocks between species allowed the identification of fixed inversion breakpoints and estimates of breakpoint reuse and lineage-specific breakpoint event segregation.
Probability distributions of Correlation and Differentials in Block Ciphers. (2005)

In this paper, we derive the probability distributions of difference propagation probabilities and input-output correlations for random functions and block ciphers, for several of them for the first time. We show that these parameters have distributions that are well-studied in the field of probability such as the normal, Poisson, Gamma and extreme value distributions. For Markov ciphers...
IN SITU THERMAL DESORPTION (ISTD) OF PCBs (1997)

A field demonstration is described in which a new in-situ thermal desorption soil remediation process (ISTD–Thermal Wells) is shown to remove high-concentration PCB contamination from clay soils. The demonstration was conducted at the Missouri Electric Works (MEW) Superfund site in Cape Girardeau, Missouri, from April 21 through June 1, 1997. For this demonstration, twelve heater/vacuum wells were completed in a multiple triangular array with a 5-foot well spacing to a depth of 12 feet. During the remediation, electrical-resistance heating and vacuum were applied to the wells for a period of 42 days. Soil temperatures were monitored throughout the experiment, and soil samples were taken with a split-spoon sampler fitted with six-inch brass coring sleeves to verify the removal of contaminants. Temperatures above 1000°F were achieved in the interwell regions, and PCB concentrations in the treated area were reduced from a maximum concentration of approximately 20,000 ppm to non-detect (i.e., <33 ppb) by EPA Method 8080. The system destruction removal efficiency (DRE) for PCBs was 99.9999998%.
Cardiac Arrest and Neurologic Recovery: Insights from the Case of Mr. Damar Hamlin (2023)

The association between brain injury after cardiac arrest and poor survival outcomes has led to longstanding pessimism. However, the publicly witnessed cardiac arrest, resuscitation, and acute management of Mr. Damar Hamlin and his favorable neurologic recovery provides some optimism. Mr. Hamlin's case highlights the neurologic advances of the last 2 decades and presents the opportunity to improve outcomes for all cardiac arrest patients in key areas: (1) effectively implementing the American Heart Association "Chain of Survival" to prevent initial brain injury and promote neuroprotection; (2) revisiting the process of neurologic prognostication and re-defining the brain recovery during the early periods, and (3) incorporating neurorehabilitation into existing cardiac rehabilitation models to support holistic recovery. ANN NEUROL 2023;93:871-876.
Data from Inhibition of LSD1 with Bomedemstat Sensitizes Small Cell Lung Cancer to Immune Checkpoint Blockade and T-Cell Killing (2023)

&lt;div&gt;AbstractPurpose:&lt;p&gt;The addition of immune checkpoint blockade (ICB) to platinum/etoposide chemotherapy changed the standard of care for small cell lung cancer (SCLC) treatment. However, ICB addition only modestly improved clinical outcomes, likely reflecting the high prevalence of an immunologically “cold” tumor microenvironment in SCLC, despite high mutational burden. Nevertheless, some patients clearly benefit from ICB and recent reports have associated clinical responses to ICB in SCLC with (i) decreased neuroendocrine characteristics and (ii) activation of NOTCH signaling. We previously showed that inhibition of the lysine-specific demethylase 1a (LSD1) demethylase activates NOTCH and suppresses neuroendocrine features of SCLC, leading us to investigate whether LSD1 inhibition would enhance the response to PD-1 inhibition in SCLC.&lt;/p&gt;Experimental Design:&lt;p&gt;We employed a syngeneic immunocompetent model of SCLC, derived from a genetically engineered mouse model harboring &lt;i&gt;Rb1/Trp53&lt;/i&gt; inactivation, to investigate combining the LSD1 inhibitor bomedemstat with anti-PD-1 therapy. &lt;i&gt;In vivo&lt;/i&gt; experiments were complemented by cell-based studies in murine and human models.&lt;/p&gt;Results:&lt;p&gt;Bomedemstat potentiated responses to PD-1 inhibition in a syngeneic model of SCLC, resulting in increased CD8&lt;sup&gt;+&lt;/sup&gt; T-cell infiltration and strong tumor growth inhibition. Bomedemstat increased MHC class I expression in mouse SCLC tumor cells &lt;i&gt;in vivo&lt;/i&gt; and augmented MHC-I induction by IFNγ and increased killing by tumor-specific T cells in cell culture.&lt;/p&gt;Conclusions:&lt;p&gt;LSD1 inhibition increased MHC-I expression and enhanced responses to PD-1 inhibition &lt;i&gt;in vivo&lt;/i&gt;, supporting a new clinical trial to combine bomedemstat with standard-of-care PD-1 axis inhibition in SCLC.&lt;/p&gt;&lt;/div&gt;
Specific residues of the cytoplasmic domains of cardiac inward rectifier potassium channels are effective antifibrillatory targets (2010)

Atrial and ventricular tachyarrhythmias can be perpetuated by up-regulation of inward rectifier potassium channels. Thus, it may be beneficial to block inward rectifier channels under conditions in which their function becomes arrhythmogenic (e.g., inherited gain-of-function mutation channelopathies, ischemia, and chronic and vagally mediated atrial fibrillation). We hypothesize that the antimalarial quinoline chloroquine exerts potent antiarrhythmic effects by interacting with the cytoplasmic domains of Kir2.1 (I(K1)), Kir3.1 (I(KACh)), or Kir6.2 (I(KATP)) and reducing inward rectifier potassium currents. In isolated hearts of three different mammalian species, intracoronary chloroquine perfusion reduced fibrillatory frequency (atrial or ventricular), and effectively terminated the arrhythmia with resumption of sinus rhythm. In patch-clamp experiments chloroquine blocked I(K1), I(KACh), and I(KATP). Comparative molecular modeling and ligand docking of chloroquine in the intracellular domains of Kir2.1, Kir3.1, and Kir6.2 suggested that chloroquine blocks or reduces potassium flow by interacting with negatively charged amino acids facing the ion permeation vestibule of the channel in question. These results open a novel path toward discovering antiarrhythmic pharmacophores that target specific residues of the cytoplasmic domain of inward rectifier potassium channels.
Production of growth factors by malignant lymphoma cell lines (1989)

Fourteen Epstein-Barr virus (EBV)-negative cell lines were raised from bone marrow (BM), peripheral blood (PB), or lymph node samples of patients with intermediate- or high-grade malignant lymphoma. The cell lines were propagated in liquid suspension culture. They contain clonogenic progenitors capable of forming lymphoma colonies in semi- solid culture medium. Cells of these lines were used to examine the growth factor requirements of their clonogenic progenitors and to assess their ability to produce their own growth factors. Two of the cell lines (OCI-Ly9 and OCI-Ly13.1) required addition of exogenous factors for colony growth. These factors were routinely provided by media conditioned by phytohemagglutinin-stimulated leukocytes (PHA- LCM). Three lines formed some and nine lines gave rise to optimal numbers of colonies without addition of growth factors. Eight of these factor-independent lines were able to function as feeder cells and promoted colony formation by both factor-dependent lines. Cell lines that displayed feeder cell function released activities into supernatants able to replace their cellular source. Some of these endogenously produced growth-promoting activities could be replaced by known hematopoietic growth factors. Both factor-dependent cell lines were cultured with recombinant IL-1 alpha, IL-2, IL-3, IL-6, and GM colony-stimulating factor (CSF) and semipurified B-cell growth factor (BCGF) interleukin-4 (IL-4). A heterogeneous response pattern was observed. Both lines formed colonies with IL-4. The colonies were comparable in frequency and size with colonies observed with (PHA-LCM). OCI-Ly9 responded to IL-6 but showed no growth with IL-2. In contrast, the TAC-positive line OCI-Ly13.1 gave rise to colonies with IL-2 while remaining unresponsive to IL-6. A moderate number of colonies was observed when cells of this line were cultured with GM-CSF. Colony formation of both lines was uninfluenced by IL1 alpha or IL-3.
Gliding edge dislocations in proteins as a mechanism for active ion transport (1978)

A mechanism for the translocation of ions across biological membranes is proposed in which transconformational changes occur in the membrane-bound ion pump. In particular, it is suggested that such conformational changes are effected by means of the propagation of crystal defects in the form of edge-dislocation glide.
Image-based reconstruction and modeling of geometry with complex reflectance in non-diffusive media (2007)

Reconstruction and modeling of 3-D geometry are among the core issues in computer vision. While geometry composed of radiometrically simple materials is currently relatively easy to reconstruct and model, geometry with complex reflectance properties presents a substantial challenge. The research work presented here is an investigation of this problem. Two methods are proposed that are specifically designed to handle geometry with complex photometric properties.
The first proposed method is designed to reconstruct large-scale geometry with arbitrary and possibly anisotropic BRDFs. Existing reconstruction techniques typically make explicit or implicit assumptions about the reflectance properties of a surface. The proposed method uses the idea of photometric ranging, where no such assumptions are necessary. In a photometric stereo-like setup of multiple images obtained from a single viewpoint under controlled illumination, photometric ranging recovers the depth directly for each camera pixel, rather than through surface normal field integration. It exploits the basic concept of radiant energy density falloff with distance from a point light source. Double-covering the incident light field allows to find sets of coincidental pairs of light directions where this can be used to align the reflected light fields and directly reconstruct the depth of a scene. Unlike photometric stereo, in photometric ranging no assumptions are required about the surface smoothness, the presence or absence of shadowing, or the nature of the BRDF, which may vary over the surface. Once the depth of a scene is known, the reflected light field can also be resampled to relight the scene, that is to render the same scene from the camera view, but under novel lighting, including nearby and distant sources. 
The second proposed method aims to model small-scale geometry of volumetric surface materials with complex reflectance. Instead of recovering the intricate geometry itself, it uses an appearance-based approach of volumetric texturing, which is a popular technique for rendering rich 3-D detail when a polygonal surface representation would be ineffective. Although efficient algorithms for rendering volumetric textures have been known for years, capturing the richness of real volumetric materials remains a challenging problem. The proposed technique generates a volumetric representation of a complex 3-D texture with unknown reflectance and structure. From the acquired reflectance data in the form of a 6-D Bidirectional Texture Function (BTF), the proposed method creates an efficient volumetric representation in the form of a stack of semi-transparent layers each representing a slice through the texture's volume. In addition to negligible storage requirements, this representation is ideally suited for hardware-accelerated real-time rendering.
Rate-visual-distortion optimized extraction with Quality Layers for scalable coding of stereo videos (2008)

The quality layers concept is proposed and adopted in the JSVM in order to ensure an optimal adaptation in a rate-distortion sense. In this paper it is extended to multiview case for scalable coding of stereo videos. Rate-visual-distortion optimized rate allocation among the views is necessary for efficient transport of 3DTV data over the Internet. This paper addresses a standard compatible approach to solve the problem. Experimental results are presented that demonstrate the effectiveness of the proposed method for several stereo videos.
Thionucleotides in transfer ribonucleic acid. Addition of N-ethylmaleimide and formation of mixed disulfides with thiol compounds (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThionucleotides in transfer ribonucleic acid. Addition of N-ethylmaleimide and formation of mixed disulfides with thiol compoundsJohn. Carbon and Harold. DavidCite this: Biochemistry 1968, 7, 11, 3851–3858Publication Date (Print):November 1, 1968Publication History Published online1 May 2002Published inissue 1 November 1968https://pubs.acs.org/doi/10.1021/bi00851a010https://doi.org/10.1021/bi00851a010research-articleACS PublicationsRequest reuse permissionsArticle Views214Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Requirement of the Fc receptor common γ‐chain forγδ T cell‐mediated promotion of murine experimental autoimmune encephalomyelitis (2005)

Immunoglobulin Fcgamma receptors (FcgammaR) are comprised of a ligand-binding alpha-chain that sometimes associates with a cell signaling common gamma-chain. These receptors comprise an important family of effector molecules that link humoral and cell-mediated adaptive immunity and regulate innate immunity. Recent animal studies suggest that FcgammaR in general, and FcR alpha-chains in particular, are required for full development of experimental autoimmune encephalomyelitis (EAE). We show here that deletion of the gamma-chain renders mice resistant to EAE, whereas deletion of the alpha-chains of FcgammaRI, FcgammaRIIB and FcgammaRIII has no protective effect. Susceptibility to EAE is fully restored in common gamma-chain-/- mice into which wild-type splenocytes are adoptively transferred, but EAE is not restored in common gamma-chain-/- mice given wild-type splenocytes depleted of gammadelta T cells. These data indicate that although the common gamma-chain is required for full development of EAE in mice, this requirement is likely FcgammaR alpha-chain-independent. Expression of the common gamma-chain by gammadelta T cells, probably in conjunction with the T cell receptor/CD3 complex, is likely the key requirement for full development of EAE.
A Comparative Severity Assessment of Impaired Balance due to Cerebellar Ataxia using Regression Models (2020)

Cerebellar ataxia (CA) refers to the impaired balance and coordination resulting from injury or degeneration of the cerebellum. Testing balance is one of the simplest means of assessing CA. This study compares instrumented assessment and clinical assessment scales of the balance test called Romberg's test. Inertial Measurement Unit (IMU) data were collected from a sensor attached to their chest of 53 subjects while they performed the test. The corresponding clinical scores were also tabulated. Using this data, 99 features were extracted to quantify acceleration, tremor and displacement of body sway. These features were filtered to identify the subset that better characterize the distinctive behavior of CA subjects. Elastic Net Regression model resulted a greater agreement (0.70 Pearson coefficient) with the clinical SARA scores. The overall results indicated that data from a single IMU sensor is sufficient to accurately assess balance in CA. The significance of this study is that evaluation of balance using Recurrence Quantification Analysis produces a comprehensive framework for the assessment of CA.
Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model (2004)

Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment.
Audit of investigation and follow-up of patients with raised monoclonal proteins. (1989)

Nine per cent of monoclonal proteins detected were not followed up at all. A urine examination to detect Bence-Jones protein was not requested in 12% of those regularly followed up. The mean age of the patients studied was 69 years, but there were eight patients under 60 years who had multiple myeloma.