gebhart/patrepeval huggingface dataset:
Hydrophobic nanoparticles improve permeability of cell-encapsulating poly(ethylene glycol) hydrogels while maintaining patternability (2010)

Cell encapsulating poly(ethylene glycol) hydrogels represent a promising approach for constructing 3D cultures designed to more closely approximate in vivo tissue environment. Improved strategies are needed, however, to optimally balance hydrogel permeability to support metabolic activities of encapsulated cells, while maintaining patternability to restore key aspects of tissue architecture. Herein, we have developed one such strategy incorporating hydrophobic nanoparticles to partially induce looser cross-linking density at the particle-hydrogel interface. Strikingly, our network design significantly increased hydrogel permeability, while only minimally affecting the matrix mechanical strength or prepolymer viscosity. This structural advantage improved viability and functions of encapsulated cells and permitted micron-scale structures to control over spatial distribution of incorporated cells. We expect that this design strategy holds promise for the development of more advanced artificial tissues that can promote high levels of cell metabolic activity and recapitulate key architectural features.
Research (2005)

summary The parts of a manuscript in the Journal of Strength and Conditioning Research are important for the practitioner to understand. In this way, the research can be better understood and used to make informed decisions in each professional's practice. The vision of such a journal was part of the commitment of the early leaders in the National Strength and Conditioning Association (NSCA) over a quarter century ago. It provides a dramatic resource today for each strength and conditioning specialist and allows the NSCA to meet its mission of “bridging the gap” between the laboratory and practitioner.
[Benign prostatic hyperplasia--treatment with sabal fruit extract. A treatment study of 1,334 patients]. (1993)

The improvement in the irritative symptoms may be considered relevant in terms of improvement in the quality of the life of the patients, and justifies this form of treatment.
High throughput low power CCMP architecture for very high speed wireless LANs (2010)

Considering the pervasion of wireless portable devices and growing trends in the use of multimedia applications, access to a high speed and, especially, a high throughput wireless channel are of significant importance. In addition to security concerns in wireless devices, deficiency in throughput and also increase in power consumption are introduced to the system by applying security. Therefore, designing a secure, high-speed wireless device with low power consumption would be a suitable response to worldwide demands. In this paper, we propose a solution to reduce encryption overhead and we almost eliminate it. Furthermore, a customized hardware architecture for Counter Mode with Cipher Block Chaining Message Authentication Code Protocol (CCMP) is proposed. This protocol is the fundamental security architecture of IEEE 802.11i standard. To improve throughput and reduce overhead, encryption is accomplished in the spare time intervals, such as DCF Inter-Frame Spaces (DIFS) used in IEEE 802.11i standard. In order to overcome the restrictions in dealing with these time intervals, a multi-core structure is proposed. Moreover, to reduce power consumption, a particular scheduler is implemented for processing cores. In the proposed architecture, we achieve up to 2Gbps throughput in the single core mode for MPDU (MAC Protocol Data Unit) and A-MSDU (Aggregated MAC Service Data Unit) input frames, and 17Gbps throughput in multi core mode for A-MPDU (Aggregated MPDU) input frames.
“Role of RNA N6‐methyladenosine methylation in endothelial response to flow” (2018)

N6‐methyladenosine (m6A) has the most abundant modification among eukaryotic mRNAs, and plays a vital role in cellular responses to many types of physiological stress. Endothelial cells (ECs) are constantly exposed in the shear stress induced by blood flow over a long period of time. Oscillatory shear (OS) can cause inflammatory response and induce altherosclerotic plaque formation. In contrast, pulsatile shear (PS) has anti‐inflammatory effect and is atheroprotective. However, the relationship between RNA modification and the effects of shear stress in endothelial cells is still unclear. In time‐series experiments on endothelial cells subjected to pulsatile and oscillatory shears, we determined m6A modifications in purified mRNA in by RNA dot blot and the expression levels of regulators of m6A by RT‐PCR and western blot. The results show that the changes of m6A modification in endothelial cells in response to different shear stresses are dynamic events and that the m6A modification of mRNA is lesser in response to OS than PS. The expression levels of m6A writers Mettl3 and Mettl14 were up‐regulated by pulsatile shear as compared with oscillatory shear and static state. In summary, this study has shown that different shear stresses can induce distinct changes of m6A modification and that Mettl3 and Mettl14 were induced by pulsatile force and may regulate RNA N6‐methyladenosine methylation in endothelial response to flow. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
Improving phase estimation with enhanced turbo decoders (2001)

This paper discusses a realistic turbo coding system when the signal phase has not been perfectly estimated. We propose improved decoding algorithms for the situations when the residual phase error can be modelled by the Gaussian probability distribution and a Markov chain, a model which can be used in many actual phase estimators. It is shown that increasing the state space of the decoders can dramatically improve the bit error probability without the need of increasing transmitted power.
Duration of Ischemia Affects Outcomes Independent of Infarct Size in Stroke (2022)

The impact of OTR on outcomes appears to be mostly through a mechanism that is independent of FI. Our findings suggest that although the field has moved toward imaging infarct core definitions of eligibility for endovascular treatment, time remains an important predictor of outcome, independent of infarct core.
Secure Java class loading (1998)

The class loading mechanism, central to Java, plays a key role in JDK 1.2 by enabling an improved security policy that is permission-based and extensible. The author concludes that JDK 1.2 has introduced a powerful and secure class loading mechanism. It not only enforces type safety and name space separation but also has a significant role in the new security architecture that supports fine grained, permission based access control. The new class loading mechanism's flexibility-through its delegation scheme and the rich set of class loader classes-gives Java applications and applets greater freedom to customize and specify how, when, and from where classes are loaded. Because the class loading mechanism is central to both the correctness and the security of the Java runtime system, we would like to model and define this mechanism, perhaps in a formal verification system. We can then obtain a formal specification and prove (or disprove) that the mechanism as currently designed is sufficient for security.
In vitro profile of the new inhaled pan-JAK inhibitor LAS194046 (2014)

Introduction : Janus kinase (JAK) is a novel anti-inflammatory target for asthma and COPD. In our internal discovery efforts we have identified LAS194046 as a potent pan-JAK inhibitor that blocks multiple cytokine receptor signaling depending on JAK1, JAK2, JAK3 and Tyk2. Aim : To characterize the enzymatic and cellular potency and the selectivity of JAK inhibitor LAS194046 compared with the reference compounds ruxolitinib and tofacitinib. Methods : JAK1, JAK2, JAK3 and Tyk2 kinase activities were assessed by HTRF assays. Inhibition of phosphorylation of downstream STAT (pSTAT) upon cytokine stimulation in human PBMCs was measured by flow cytometry: IL4-induced pSTAT6 (via JAK1/3), GM-CSF-induced pSTAT5 (via JAK2/2), IFNα-induced pSTAT1 (via JAK1/Tyk2) and IL12-induced pSTAT4 (via Jak2/Tyk2).The selectivity profile was assessed at 10 mM in a Millipore Kinase Profiler panel comprising 38 kinases. Results : LAS194046 inhibits JAK1, JAK2 and JAK3 kinase activity with similar potency to ruxolitinib and tofacitinib (IC 50 :>10 nM). The compound also inhibits Tyk2 with higher potency than tofacitinib (8-fold) and lower than ruxolitinib (15-fold less). In PBMCs the three compounds inhibits JAK1/3 and JAK1/Tyk2 assays (IC 50 50 Conclusions : LAS194046 is a potent pan-JAK inhibitor, that inhibits JAK1/2/3 and Tyk2 activities and associated cytokine receptor signaling. The promising in vitro profile of LAS194046 supports to further characterize its ADME properties and in vivo efficacy by inhaled route.
Enzymatic Dissociation Induces Transcriptional and Proteotype Bias in Brain Cell Populations (2020)

Different cell isolation techniques exist for transcriptomic and proteotype profiling of brain cells. Here, we provide a systematic investigation of the influence of different cell isolation protocols on transcriptional and proteotype profiles in mouse brain tissue by taking into account single-cell transcriptomics of brain cells, proteotypes of microglia and astrocytes, and flow cytometric analysis of microglia. We show that standard enzymatic digestion of brain tissue at 37 °C induces profound and consistent alterations in the transcriptome and proteotype of neuronal and glial cells, as compared to an optimized mechanical dissociation protocol at 4 °C. These findings emphasize the risk of introducing technical biases and biological artifacts when implementing enzymatic digestion-based isolation methods for brain cell analyses.
An MPSoC Performance Estimation Framework Using Transaction Level Modeling (2007)

To use the tremendous hardware resources available in next generation multiprocessor systems-on-chip (MPSoC) efficiently, rapid and accurate design space exploration (DSE) methods are needed to evaluate the different design alternatives. In this paper, we present a framework that makes fast simulation and performance evaluation of MPSoC possible early in the design flow, thus reducing the time-to-market. In this framework and within the transaction level modeling (TLM) approach, we present a new definition of the timed programmer's view (PVT) level by introducing two complementary modeling sublevels. The first one, PVT transaction accurate (PVT-TA), offers a high simulation speedup factor over the cycle accurate bit accurate (CABA) level modeling. The second one, PVT event accurate (PVT-EA), provides a better accuracy with a still acceptable speedup factor. An MPSoC platform has been developed using these two sublevels including performance estimation models. Simulation results show that the combination of these two sublevels gives a high simulation speedup factor of up to 18 with a negligible performance estimation error margin.
Phage Display-Derived Binders Able to Distinguish Listeria monocytogenes from Other Listeria Species (2013)

The objective of this study was to produce phage display-derived binders with the ability to distinguish Listeria monocytogenes from other Listeria spp., which may have potential utility to enhance detection of Listeria monocytogenes. To obtain binders with the desired binding specificity a series of surface and solution phage-display biopannings were performed. Initially, three rounds of surface biopanning against gamma-irradiated L. monocytogenes serovar 4b cells were performed followed by an additional surface biopanning round against L. monocytogenes 4b which included prior subtraction biopanning against gamma-irradiated L. innocua cells. In an attempt to further enhance binder specificity for L. monocytogenes 4b two rounds of solution biopanning were performed, both rounds included initial subtraction solution biopanning against L. innocua. Subsequent evaluations were performed on the phage clones by phage binding ELISA. All phage clones tested from the second round of solution biopanning had higher specificity for L. monocytogenes 4b than for L. innocua and three other foodborne pathogens (Salmonella spp., Escherichia coli and Campylobacter jejuni). Further evaluation with five other Listeria spp. revealed that one phage clone in particular, expressing peptide GRIADLPPLKPN, was highly specific for L. monocytogenes with at least 43-fold more binding capability to L. monocytogenes 4b than to any other Listeria sp. This proof-of-principle study demonstrates how a combination of surface, solution and subtractive biopanning was used to maximise binder specificity. L. monocytogenes-specific binders were obtained which could have potential application in novel detection tests for L. monocytogenes, benefiting both the food and medical industries.
Bacillus anthracisEdema Toxin Suppresses Human Macrophage Phagocytosis and Cytoskeletal Remodeling via the Protein Kinase A and Exchange Protein Activated by Cyclic AMP Pathways (2009)

Bacillus anthracis, the etiological agent of anthrax, is a gram-positive spore-forming bacterium. It produces edema toxin (EdTx), a powerful adenylate cyclase that increases cyclic AMP (cAMP) levels in host cells. Because other cAMP-increasing agents inhibit key macrophage (MPhi) functions, such as phagocytosis, it was hypothesized that EdTx would exhibit similar suppressive activities. Our previous GeneChip data showed that EdTx downregulated MPhi genes involved in actin cytoskeleton remodeling, including protein kinase A (PKA). To further examine the role of EdTx during anthrax pathogenesis, we explored the hypothesis that EdTx treatment leads to deregulation of the cAMP-dependent PKA system, resulting in impaired cytoskeletal functions essential for MPhi activity. Our data revealed that EdTx significantly suppressed human MPhi phagocytosis of Ames spores. Cytoskeletal changes, such as decreased cell spreading and lowered F-actin content, were also observed for toxin-treated MPhis. Further, EdTx altered the protein levels and activity of PKA and exchange protein activated by cAMP (Epac), a recently identified cAMP-binding molecule. By using PKA- and Epac-selective cAMP analogs, we confirmed the involvement of both pathways in the inhibition of MPhi functions elicited by EdTx-generated cAMP. These results suggested that EdTx weakened the host immune response by increasing cAMP levels, which then signaled via PKA and Epac to cripple MPhi phagocytosis and interfered with cytoskeletal remodeling.
Machine Translation Evaluation Meets Community Question Answering (2016)

We explore the applicability of machine translation evaluation (MTE) methods to a very different problem: answer ranking in community Question Answering. In particular, we adopt a pairwise neural network (NN) architecture, which incorporates MTE features, as well as rich syntactic and semantic embeddings, and which efficiently models complex non-linear interactions. The evaluation results show state-of-the-art performance, with sizeable contribution from both the MTE features and from the pairwise NN architecture.
Viscoelastic properties of polybutadiene rings (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTViscoelastic properties of polybutadiene ringsJacques RooversCite this: Macromolecules 1988, 21, 5, 1517–1521Publication Date (Print):May 1, 1988Publication History Published online1 May 2002Published inissue 1 May 1988https://pubs.acs.org/doi/10.1021/ma00183a049https://doi.org/10.1021/ma00183a049research-articleACS PublicationsRequest reuse permissionsArticle Views844Altmetric-Citations146LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Neutrophil-Mediated Mycobacteriocidal Immunity in the Lung duringMycobacterium bovisBCG Infection in C57BL/6 Mice (2002)

Although neutrophils have been identified as sources of inflammatory cytokines and chemokines, little is known about their immunologic function during mycobacterial infection in the lungs. In this study, we examined the growth of Mycobacterium bovis BCG in the lungs under experimental conditions that altered neutrophil recruitment to the lungs. Depletion and recruitment of neutrophils was associated with respective increases and decreases in M. bovis BCG growth. Thus, neutrophils may enhance mycobacteriocidal immunity in the lungs.
Comparison of the rate of urea degradation by acid urease in European and American wine and the relationships between the degradation rate and the wine constituents (2001)

The rate of urea degradation by acid urease in European and American wines were compared and it was shown that the average degradation rate in European wine was 6-9 fold higher than that of American wine, in both white and rose wine. Consequently, urea reduction by urease treatment is more difficult in American wine than in European wine. There was little difference in the urea degradation rates for red wine. A significant negative correlation was observed between fluoride concentration and the degradation rate, in addition, a significant difference in fluoride concentration was found in European and American white/rose wines. Therefore, it was suggested that the high concentration of fluoride in American wine made urea reduction difficult. A significant positive correlation was found between Llactate concentration and the degradation rate.
Quantum Boltzmann equation for atomic hydrogen (1985)

The quantum Boltzmann equation governing the evolution of the one-body internal density matrix of an atomic hydrogen (or alkali) gas is established. Indistinguishability of both nuclei and electrons explains the presence in the collision term of a large number (eleven) of « exchange cross sections ». The different collision terms fall into two categories : these which contribute to the damping of perturbations (similar to the usual collision term in the classical regime) and those which are Hamiltonian like and originate from the « identical spin rotation effect ». Due to those numerous exchange terms, all transport properties in atomic hydrogen are very sensitive to the internal state of the gas.
Clinical and genetic studies of fatal familial insomnia (1995)

We report a 42-year-old man who, for 8 months, had intermittent motor abnormalities and mild difficulty falling asleep. A diagnosis of fatal familial insomnia (FFI) became evident over the next 6 months when he developed progressive insomnia, myoclonus, sympathetic hyperactivity, and dementia. The amyloid or prion protein (PrP) genotype showed features typically seen in FFI, with a 178Asn mutation and a 129Met polymorphism. There was also a deletion of one octapeptide repeat, suggesting that the association of 178Asn mutation with the 129Met polymorphism is not due to "founder effect." Western immunoblot showed a trace of protease-resistant PrP in the thalamus--which had the most significant neuronal loss and gliosis--a moderate amount of PrP in the fronto-temporal area, and no detectable protein elsewhere in the brain. Endocrine studies showed that a circadian modulation of hormonal levels could be maintained despite a near-total absence of sleep. Administration of gamma-hydroxybutyrate induced a remarkable increase in slow-wave sleep.
RNS60, a Novel Therapeutic, Modifies T-Helper Cells and Inhibits Experimental Allergic Encephalomyelitis: Implications for Treating Multiple Sclerosis (P05.116) (2012)

Objective: Despite intense investigations, no fully effective treatment is available to halt the progression of multiple sclerosis (MS). The current study explores the efficacy of RNS60, a novel anti-inflammatory therapeutic, in a relapsing-remitting disease model of MS. Background MS is a chronic autoimmune demyelinating disease of the central nervous system (CNS). Existing therapies are limited and have significant side effects. Since RNS60 has demonstrated broad anti-inflammatory efficacy and extraordinarily low toxicity in multiple inflammatory disease models, we tested its effectiveness in relapsing-remitting experimental allergic encepahalomyelitis (RR-EAE). Design/Methods: RNS60 was generated by subjecting normal saline to Taylor-Couette-Poiseuille flow and cavitation forces under elevated oxygen pressure. RR-EAE was induced by adoptive transfer of MBP-primed T cells. Recipient mice were treated with RNS60 intraperitoneally from the onset of the acute or relapsing phase through 42 days post transfer. Alternatively, primed T cells were treated ex vivo prior to injection into mice. Various disease parameters including clinical score, T helper cell markers and cytokine expression, infiltration of mononuclear cells, and demyelination in the CNS were assessed following treatment. Results: Treatment with RNS60 (100-300 μL) ameliorated clinical symptoms and disease progression of EAE in recipient mice (n=5, p Conclusions: RNS60 significantly alters the disease course of EAE via immune-modulation. The ability to influence disease parameters with a physically-modified saline opens possibilities for the generation of a new class of therapeutics for MS. Supported by: Revalesio Corporation, Tacoma, WA, has supported this study. Disclosure: Dr. Mondal has nothing to disclose. Dr. Ghosh has received personal compensation for activities with Revalesio Corporation as an employee. Dr. Watson has received personal compensation for activities with Revalesio Corporation as an employee. Dr. Pahan has received research support from Revalesio Corporation.
Ring-Closing Metathesis Reactions on Azinium Salts: Straightforward Access to Quinolizinium Cations and Their Dihydro Derivatives (2009)

The ring-closing metathesis reaction of 1-butenyl-2-vinylpyridinium salts and 2-butenyl-1-vinylpyridinium salts using Grubbs second generation and Hoveyda-Grubbs catalysts proved to be an efficient approach to 3,4-dihydro- and 1,2-dihydroquinolizinium salts and the corresponding quinolizinium derivatives by an improved thermal oxidation in the presence of Pd/C without solvent. A comparative study showed that the quinolizinium system was obtained in better yields through the 3,4-dihydroquinolizinium route, thus allowing the synthesis of quinolizinium derivatives or improvements in the yields of some examples reported previously.