1370 URINE ANALYSIS OF CONFIRMED UTI PATIENTS REVEAL HIGHER LEVELS OF CXC CHEMOKINES COMPARED TO PATIENTS WITH LUTS WITHOUT UTI (2011)

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Kidney & Bladder1 Apr 20111370 URINE ANALYSIS OF CONFIRMED UTI PATIENTS REVEAL HIGHER LEVELS OF CXC CHEMOKINES COMPARED TO PATIENTS WITH LUTS WITHOUT UTI Pradeep Tyagi, Vikas Tyagi, Harvey Qu, Kenneth Peters, Yao-Chi Chuang, Hsin-Tzu Liu, Hann-Chorng Kuo, and Michael Chancellor Pradeep TyagiPradeep Tyagi Royal Oak, MI More articles by this author , Vikas TyagiVikas Tyagi Royal Oak, MI More articles by this author , Harvey QuHarvey Qu Rochester, MI More articles by this author , Kenneth PetersKenneth Peters Royal Oak, MI More articles by this author , Yao-Chi ChuangYao-Chi Chuang Kaohsiung, Taiwan More articles by this author , Hsin-Tzu LiuHsin-Tzu Liu Hualien, Taiwan More articles by this author , Hann-Chorng KuoHann-Chorng Kuo Hualien, Taiwan More articles by this author , and Michael ChancellorMichael Chancellor Royal Oak, MI More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1194AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Reliable prediction of UTI is hindered by the lack of non-invasive predictive urine biomarkers. Past studies have focused on IL-8 (CXCL-8) with the recruitment of neutrophils after bacterial invasion of uroepithelial cells. In the present study, we investigated whether other members of IL-8 family of chemokines i.e. CXC chemokines are also elevated following UTI and whether they can together form a specific multi-marker panel that is more useful as a surrogate marker for rapid laboratory diagnosis of UTI and prevention of urosepsis. METHODS Single time point urine specimens were collected from 42 patients with confirmed UTI (+ve bacterial culture report) prior to initiation of antibiotic treatment and compared them with 4 other cohorts of either sex without any infection ( ruled out by urine dipstick test) in the age range of (25–90 yrs). There were Overactive bladder (n= 115), interstitial cystitis/painful bladder syndrome ( IC/PBS); n=59 and asymptomatic controls (n=44). Differentiation between IC/ PBS, OAB-wet and OAB-dry was based on absence or presence of pain symptoms and a 3-day voiding diary. Ten chemokines in urine were measured by MILLIPLEX™ MAP Human Cytokine/Chemokine multiplex immunoassay and ELISA. Multivariate logistic regression was used to determine association and Kruskal-wallis ANOVA was used to check for significance. RESULTS The multivariate regression analysis highlighted that existence of UTI was associated with elevated CXCL-10 levels (odds ratio [OR]:1.05; 95% CI, (1.0187, 1.0930), p=0.002). Levels of CXCL-10, CXCL1 and IL-8 in confirmed UTI urine were also higher than in OAB; (1203±537.2 vs 39.31±17.51pg/ml), (96.16±28.19 vs 11.15±7.50pg/ml) and (113±29.45 vs 29.1±13.54pg/ml), respectively. Urine levels of non-CXC chemokines like MCP-1, IL-1ra and PDGF were higher in population without underlying urinary tract infection such as IC/PBS and control subjects, and higher urinary NGF were noted in OAB wet patients. CONCLUSIONS Discriminatory multivariate modeling of 10 urinary chemokines identified UTI associated CXC chemokines in urine and thereby demonstrated the utility of mathematical modeling for identifying disease-associated urine biomarkers. Although a single marker such as urinary IL-8 may be a sensitive for UTI, but it is poorly specific as it is also elevated in a variety of other diseases. A mutimarker panel that also includes CXCL-10 and CXCL-1 can be used as a urinary finger print for UTI with increased specificity. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e547 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Pradeep Tyagi Royal Oak, MI More articles by this author Vikas Tyagi Royal Oak, MI More articles by this author Harvey Qu Rochester, MI More articles by this author Kenneth Peters Royal Oak, MI More articles by this author Yao-Chi Chuang Kaohsiung, Taiwan More articles by this author Hsin-Tzu Liu Hualien, Taiwan More articles by this author Hann-Chorng Kuo Hualien, Taiwan More articles by this author Michael Chancellor Royal Oak, MI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...