gebhart/patrepeval huggingface dataset:
Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function (2012)

A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs into the intended transcriptional unit potently stimulated reporter activity when the inserted test sequence contained a 3' splice site (ss). We show that two human sequences, originally reported to be internal ribosome entry sites and later to instead be promoters, mimic both types of element in dicistronic reporter assays by causing these cryptic readthrough transcripts to splice in patterns that allow efficient translation of the downstream cistron. Introduction of test sequences containing 3' ss into monocistronic luciferase reporter vectors widely used in the study of transcriptional regulation also created the false appearance of promoter function via the same mechanism. Across a large number of variants of these plasmids, we found a very highly significant correlation between reporter activity and levels of such spliced readthrough transcripts. Computational estimation of the frequency of cryptic 3' ss in genomic sequences suggests that misattribution of cis-regulatory function may be a common occurrence.
Mineralogical and geochemical analysis of Fe-phases in drill-cores from the Triassic Stuttgart Formation at Ketzin CO2 storage site before CO2 arrival (2017)

Author: Kasina, Monika et al.; Genre: Journal Article; Finally published : 2017; Open Access; Title: Mineralogical and geochemical analysis of Fe-phases in drill-cores from the Triassic Stuttgart Formation at Ketzin CO2 storage site before CO2 arrival
The new alchemists (1999)

This work is an examination in words and pictures of the way particular individuals, like Anita Roddick (Body Shop), Tim Waterstone (Waterstones) or Charles Dunstone (Carphone Warehouse) have created businesses/careers from nothing but their own drive and talent.
Strategies to incorporate a fluorinated acrylate monomer into polymer particles: from particle morphology to film morphology and anticorrosion properties (2022)

Abstract Four strategies to incorporate a fluorinated monomer (perfluoro octyl acrylate, POA) into a waterborne polymeric dispersion are investigated. Due to the very low water solubility of the POA monomer, three of the strategies use miniemulsion droplets containing the whole POA monomer in the initial charge. The rest of the comonomers of the formulation (methyl methacrylate, MMA, and n-butyl acrylate, BA) are partially incorporated in the initial miniemulsion or fed to the reactor as a preemulsion. In the fourth strategy, a conventional seeded semibatch emulsion polymerization is carried out using cyclodextrin in the seed and feeding the POA/MMA/BA preemulsion to the reactor. Each process strategy led to a distinct particle morphology and hence a particular film morphology. We found that the strategy that produced core–shell particles with the core composed by pure polyPOA yielded the films that showed the best corrosion protection as measured in salt-spray test (1200 h standing without damage).
Test performance evaluation of SARS-CoV-2 serological assays (2020)

Our evaluation showed heterogeneous assay performance. Reader training is key to reliable LFA performance, and can be tailored for survey goals. Informed use of serology will require evaluations covering the full spectrum of SARS-CoV-2 infections, from asymptomatic and mild infection to severe disease, and later convalescence. Well-designed studies to elucidate the mechanisms and serological correlates of protective immunity will be crucial to guide rational clinical and public health policies.
Current and Future Drug Targets in Weight Management (2010)

Obesity will continue to be one of the leading causes of chronic disease unless the ongoing rise in the prevalence of this condition is reversed. Accumulating morbidity figures and a shortage of effective drugs have generated substantial research activity with several molecular targets being investigated. However, pharmacological modulation of body weight is extremely complex, since it is essentially a battle against one of the strongest human instincts and highly efficient mechanisms of energy uptake and storage. This review provides an overview of the different molecular strategies intended to lower body weight or adipose tissue mass. Weight-loss drugs in development include molecules intended to reduce the absorption of lipids from the GI tract, various ways to limit food intake, and compounds that increase energy expenditure or reduce adipose tissue size. A number of new preparations, including combinations of the existing drugs topiramate plus phentermine, bupropion plus naltrexone, and the selective 5-HT(2C) agonist lorcaserin have recently been filed for approval. Behind these leading candidates are several other potentially promising compounds and combinations currently undergoing phase II and III testing. Some interesting targets further on the horizon are also discussed.
Mini-probes: a new dimension in offshore in situ testing (2003)

Recent development of a series of miniature probes for acquiring geoscience data is described. Because of the small probe size, pushing mechanisms are also scaled down (1000 kg, typically). Handling equipment onboard oceanographic research vessels is suitable for deployment of the self-contained miniprobe systems. Installation of the systems on remotely-operated-vehicles also appears feasible. Penetrations to 12.2 m have been achieved.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
4-Alkyloxyimino Derivatives of Uridine-5′-triphosphate: Distal Modification of Potent Agonists as a Strategy for Molecular Probes of P2Y2, P2Y4, and P2Y6Receptors (2014)

Extended N(4)-(3-arylpropyl)oxy derivatives of uridine-5'-triphosphate were synthesized and potently stimulated phospholipase C stimulation in astrocytoma cells expressing G protein-coupled human (h) P2Y receptors (P2YRs) activated by UTP (P2Y2/4R) or UDP (P2Y6R). The potent P2Y4R-selective N(4)-(3-phenylpropyl)oxy agonist was phenyl ring-substituted or replaced with terminal heterocyclic or naphthyl rings with retention of P2YR potency. This broad tolerance for steric bulk in a distal region was not observed for dinucleoside tetraphosphate agonists with both nucleobases substituted. The potent N(4)-(3-(4-methoxyphenyl)-propyl)oxy analogue 19 (EC50: P2Y2R, 47 nM; P2Y4R, 23 nM) was functionalized for chain extension using click tethering of fluorophores as prosthetic groups. The BODIPY 630/650 conjugate 28 (MRS4162) exhibited EC50 values of 70, 66, and 23 nM at the hP2Y2/4/6Rs, respectively, and specifically labeled cells expressing the P2Y6R. Thus, an extended N(4)-(3-arylpropyl)oxy group accessed a structurally permissive region on three Gq-coupled P2YRs, and potency and selectivity were modulated by distal structural changes. This freedom of substitution was utilized to design of a pan-agonist fluorescent probe of a subset of uracil nucleotide-activated hP2YRs.
Reduction of interference fringes in small multipass absorption cells by pressure modulation (1990)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Alan Fried, James R. Drummond, Bruce Henry, and Jack Fox, "Reduction of interference fringes in small multipass absorption cells by pressure modulation," Appl. Opt. 29, 900-902 (1990) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
The relative physical and thermal properties of polyurethane elastomers. Effect of chain extenders derived from dihydroxynaphthalene (1997)

Abstract Polyurethane elastomers were synthesized based on polyols such as poly(caprolactonediol) and poly(tetramethylene glycol), diisocyanates such as diphenylmethane‐4,4′‐diisocyanate (MDI) and dicyclohexylmethane‐4,4′‐diisocyanate (HMDI), and two dihydroxynaphthalenes (DHNs) and their brominated derivatives used as chain extenders. The effects of the chemical structures of the chain extenders on the physical and thermal properties of the polyurethanes were investigated. Because of the higher degree of phase mixing of the 1,5‐DHN unit, the 1,5‐DHN‐based polyurethanes have higher T g values and higher dynamic properties than 2,7‐DHN‐based polyurethanes. Introduction of bromine atoms increased the flame retardance. However, the large free volume of bromine atoms restricted the rotation of DHNs, thus, directly decreasing the degree of phase‐mixing and crystallinity of the polyurethanes. Introducing bromine atoms also decreased the mechanical and dynamic properties and increased the solubility of the polyurethanes.
Polymer Chemistry of Synthetic Elastomers (1968)

Polymer chemistry of synthetic elastomers , Polymer chemistry of synthetic elastomers , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Results on a direct digital receiver operated with fast learning networks (2003)

In this paper, learning algorithms are used to decode QPSK modulated signals in a direct conversion microwave/millimetre wave receiver using an application specific six port module. Two different algorithms, K-Means and Online Bayesian Network, are considered for operation of the decoder to recover IQ data from modulated signals. Bit Error Rate (BER) vs. Noise level (Eb/No) results are presented including the case where the Local Oscillator (LO) is not locked to carrier signal.
Newfangled Topical Film-Forming Solution for Facilitated Antifungal Therapy: Design, Development, Characterization, and In Vitro Evaluation (2023)

Luliconazole is a broad-spectrum topical antifungal agent that acts by altering the synthesis of fungi cell membranes. Literature suggests that the recurrence of fungal infection can be avoided by altering the pH of the site of infection. Studies have also suggested that fungi thrive by altering skin pH to be slightly acidic, i.e., pH 3-5. The current study is aimed to design, develop, characterize, and evaluate an alkaline pH-based antifungal spray solution for antifungal effects. Luliconazole was used as an antifungal agent and an alkaline spray was formulated for topical application by using Eudragit RS 100, propylene glycol (PG), water, sodium bicarbonate, and ethanol via solubilization method. Herein, sodium bicarbonate was used as an alkalizing agent. Based on DSC, FTIR, PXRD, scanning electron microscopy (SEM), and rheological analysis outcomes, the drug (luliconazole) and polymer were found to be compatible. F-14 formulation containing 22% Eudragit RS 100 (ERS), 1.5% PG, and 0.25% sodium bicarbonate was optimized by adopting the quality by design approach by using design of experiment software. The viscosity, pH, drying time, volume of solution post spraying, and spray angle were, 14.99 ± 0.21 cp, 8 pH, 60 s, 0.25 mL ± 0.05 mL, and 80 ± 2, respectively. In vitro drug diffusion studies and in vitro antifungal trials against <i>Candida albicans</i> revealed 98.0 ± 0.2% drug diffusion with a zone of inhibition of 9 ± 0.12 mm. The findings of the optimized luliconazole topical film-forming solution were satisfactory, it was compatible with human skin, and depicted sustained drug release that suggests promising applicability in facilitated topical antifungal treatments.
HB-EGF promotes intestinal restitution by affecting integrin–extracellular matrix interactions and intercellular adhesions (2013)

Restitution is a critical form of intestinal epithelial cell (IEC) healing. We have previously shown that heparin-binding epidermal-like growth factor (HB-EGF) is necessary for IEC restitution; however, the mechanisms by which HB-EGF promotes restitution remain poorly understood. This study was designed to investigate whether HB-EGF promotes intestinal restitution by affecting integrin-extracellular matrix (ECM) interactions and intercellular adhesions. The effect of HB-EGF administration was examined in a murine necrotizing enterocolitis (NEC) model in vivo and an IEC line scrape-wound healing model in vitro. We evaluated the effect of HB-EGF on the expression of integrins, E-cadherin/β-catenin, and integrin α5β1-dependent cell-ECM interactions. We found that HB-EGF promoted intestinal restitution and the expression of integrin α5β1. HB-EGF promoted integrin α5β1-dependent cell adhesion and spreading. In addition, HB-EGF decreased the expression E-cadherin/β-catenin, via the activation of v-erb-b2 erythroblastic leukemia viral oncogene homolog (ErbB-1). We conclude that HB-EGF promotes intestinal restitution by affecting integrin-ECM interactions and intercellular adhesions.
Influence of sterilisation methods on collagen-based devices stability and properties (2014)

Sterilisation is essential for any implantable medical device in order to prevent infection in patients. The selection of the most appropriate sterilisation method depends on the nature and the physical state of the material to be sterilised; the influence of the sterilisation method on the properties of the device; and the type of the potential contaminant. In this context, herein we review the influence of ethylene oxide, γ-irradiation, e-beam irradiation, gas plasma, peracetic acid and ethanol on structural, biomechanical, biochemical and biological properties of collagen-based devices. Data to-date demonstrate that chemical approaches are associated with cytotoxicity, whilst physical methods are associated with degradation, subject to the device physical characteristics. Thus, the sterilisation method of choice is device dependent.
A new method for the design of two-channel perfect-reconstruction linear-phase FIR filter banks (2000)

An efficient two-step approach is presented for designing two-channel perfect-reconstruction linear-phase FIR filter banks. The first step involves finding a good solution by using an iterative procedure. This iterative procedure is generated by properly modifying the Lagrange-Newton method proposed by Horng and Willson (1992). In the second step, the resulting solution is then used as a good initial solution for further optimization that is carried out by the second algorithm of Dutta and Vidyasagar (1977). Several examples are included illustrating the efficiency of the proposed approach and the resulting two-channel filter banks.
Two Human Neonatal IgM Antibodies Encoded by Different Variable-Region Genes Bind the Same Linear Peptide: Evidence for a Stereotyped Repertoire of Epitope Recognition (1999)

Two monoclonal IgM Abs have been produced from lymphocytes isolated from two human umbilical cord bloods. These mAbs recognize a conformational epitope present in a CNBr digestion fraction of lactoferrin. Linear epitopes recognized by each mAb were selected from several phage display peptide libraries. In each case, phages displaying a peptide with a motif defined by [WF],G,[EQS],N were recovered. Phages displaying that motif bound equally well to either mAb but did not bind to control IgM. A peptide bearing this motif competed with the phage-displayed peptides for binding to either mAb. The same peptide also competes with a component of the CNBr digestion fraction of lactoferrin for Ab binding in ELISA. The Abs use different families of VH, JH, and VK gene cassettes but use the same JK cassette. All segments are virtually identical to their germline gene counterparts. This work provides further evidence that certain innate specificities are stereotyped among individuals.
Adenine Derived Inhibitors of the Molecular Chaperone HSP90—SAR Explained Through Multiple X‐Ray Structures. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Testing and spot-checking of data streams (2000)

We consider the tasks of testing and spot-checking for data streams. These testers and spotcheckers are potentially useful in real-time or near real-time applications that process huge datasets. Crucial aspects of the computational model include the space complexity of the testers and spotcheckers (ideally much lower than the size of the input stream) and the number of passes that the tester or spot-checker must make over the input stream (ideally one, because the original stream may be too large to store for a second pass). A sampling-tester [GGR98] for a property P samples some (but usually not all) of its input and, with high probability, outputs PASS if the input has property P and FAIL if the input is far from having P , for an appropriate sense of &amp;quot;far.&amp;quot; A streaming-tester for a property P of one or more input streams takes as input one or more data streams and, with high probability, outputs PASS if the streams have property P and FAIL if the streams are far from havin...
Blurred Lines (2017)

Where writers like James, Howells, and Wharton disdained illustrations, regarding them as a distraction from the psychological realism of their fiction, Jack London welcomed the visual embellishment. He recognized how pictures helped sell books and magazines. Throughout his career he lobbied for favorite artists and criticized others, argued for the usefulness of pictures as reading guides and marketing tools, and requested pieces of original artwork for his private collection. His motivation, however, wasn’t strictly commercial. The discursive and visual elements surrounding any publication inevitably leave their impress on our experience of the work. In London’s case, they suggest a more collaborative relationship between texts and paratexts than has previously been recognized. Equally important, they point to a postmodern self-referentiality that becomes increasingly pronounced as London’s career progresses.
Specificity of Human Milk Bile Salt (1985)

A crude preparation of the bile salt-stimulated lipase in human milk did not differentiate between the primary and secondary esters of several synthetic triacylglycerols. The dioleoyl ester of 2,3-butanediol was also digested, unequivocal confirmation of the absence of positional specificity. Preferential release of 18:2 relative to 16:0 and 18:1 as compared to 18:1t (trans) was observed. The lipase did not differentiate between 16:0 and 18:1 or 16:0 and 18:0.
The Reproductive Biology of Hippelates collusor (Diptera: Chloropidae). I. Morphology of the Reproductive Systems, with Notes on Physiological Aging1,2 (1967)

The external male genitalia are composed of the genital capsule, the articulated first surstyli, the nonarticulated second surstyli, the aedeagus, the parameres, and the ejaculatory apodeme. The internal male reproductive system consists of the ejaculatory duct, vas efferens, accessory glands, and the testes. The ovipositor is the primary external genitalic structure of the female. The vagina, seminal receptacle, a paired coiled spermatheca, common oviduct, lateral oviducts, and the ovaries comprise the internal reproductive system of the female. Parous females were distinguished from nulliparous ones by a distended common oviduct, loose appearing ovarioles, follicle cell residues in the ovarioles, and sperm in the spermatheca.
Chloramphenicol Resurrected: A Journey from Antibiotic Resistance in Eye Infections to Biofilm and Ocular Microbiota (2019)

The advent of multidrug resistance among pathogenic bacteria is devastating the worth of antibiotics and changing the way of their administration, as well as the approach to use new or old drugs. The crisis of antimicrobial resistance is also due to the unavailability of newer drugs, attributable to exigent regulatory requirements and reduced financial inducements. The emerging resistance to antibiotics worldwide has led to renewed interest in old drugs that have fallen into disuse because of toxic side effects. Thus, comprehensive efforts are needed to minimize the pace of resistance by studying emergent microorganisms and optimize the use of old antimicrobial agents able to maintain their profile of susceptibility. Chloramphenicol is experiencing its renaissance because it is widely used in the treatment and prevention of superficial eye infections due to its broad spectrum of activity and other useful antimicrobial peculiarities, such as the antibiofilm properties. Concerns have been raised in the past for the risk of aplastic anemia when chloramphenicol is given intravenously. Chloramphenicol seems suitable to be used as topical eye formulation for the limited rate of resistance compared to fluoroquinolones, for its scarce induction of bacterial resistance and antibiofilm activity, and for the hypothetical low impact on ocular microbiota disturbance. Further in-vitro and in vivo studies on pharmacodynamics properties of ocular formulation of chloramphenicol, as well as its real impact against biofilm and the ocular microbiota, need to be better addressed in the near future.
Liver uptake of gold nanoparticles after intraperitoneal administration in vivo: A fluorescence study (2011)

The fluorescence intensity of GNPs varied with the number, size and shape of particles and with the ratio of surface area to volume in a given sample. Fluorescence intensity changes during infusion depended on the size and shape of GNPs, with smaller particles experiencing larger changes during the infusion time in addition to the quenching produced by the larger GNPs. It is likely that smaller particles, which have a much higher ratio of surface area to volume compared to larger particles, are more prone to aggregation and surface interaction with biological components. This study suggests that fluorescence intensity can be used to evaluate bioaccumulation and the toxicity of gold nanoparticles in rats.
Impact of radiotherapy delay following biopsy for patients with unresected glioblastoma (2022)

In patients with unresected glioblastoma, a longer time interval from biopsy to radiotherapy does not appear to be associated with worse overall survival. However, external validation of these findings is necessary given that selection bias is a significant limitation of this study.
Advanced Industrial Lubricants and Future Development Trends of Tribo-Systems for Tribological Performance Evaluation (2023)

It is possible to solve challenges in the global automotive and manufacturing industries by using a multidisciplinary approach to advanced industrial lubricants, their tribological performance evaluation, and new surface engineering techniques for prospective tribo-systems [...]
One more step toward a warmer Arctic (2005)

This study was motivated by a strong warming signal seen in mooring‐based and oceanographic survey data collected in 2004 in the Eurasian Basin of the Arctic Ocean. The source of this and earlier Arctic Ocean changes lies in interactions between polar and sub‐polar basins. Evidence suggests such changes are abrupt, or pulse‐like, taking the form of propagating anomalies that can be traced to higher‐latitudes. For example, an anomaly found in 2004 in the eastern Eurasian Basin took ∼1.5 years to propagate from the Norwegian Sea to the Fram Strait region, and additional ∼4.5–5 years to reach the Laptev Sea slope. While the causes of the observed changes will require further investigation, our conclusions are consistent with prevailing ideas suggesting the Arctic Ocean is in transition towards a new, warmer state.
Coherent population trapping for the realization of a small, stable, atomic clock (2003)

The coherent population trapping phenomenon (CPT) has created interesting avenues in the practical realization of atomic frequency standards using laser optical pumping. In practice two different types of standards can be implemented: a passive clock based on the dark state created in the ensemble by the CPT phenomenon and a maser based on the ground state oscillating magnetization induced by the same phenomenon. The paper describes the basic physics involved in the approach and summarizes the progress made thus far in the practical implementation of such frequency standards.