gebhart/patrepeval huggingface dataset:
The Clinical Pharmacogenomics Implementation Consortium: CPIC Guideline for SLCO1B1 and Simvastatin-Induced Myopathy (2012)

Cholesterol reduction from statin therapy has been one of the greatest public health successes in modern medicine. Simvastatin is among the most commonly used prescription medications. A non-synonymous coding single-nucleotide polymorphism (SNP), rs4149056, in SLCO1B1 markedly increases systemic exposure to simvastatin and the risk of muscle toxicity. This guideline explores the relationship between rs4149056 (c.521T>C, p.V174A) and clinical outcome for all statins. The strength of the evidence is high for myopathy with simvastatin. We limit our recommendations accordingly.
Purification and Characterization of a Novel Metalloproteinase, Acurhagin, from Agkistrodon acutus Venom (2002)

Summary Acurhagin, a high-molecular mass hemorrhagic metalloproteinase, was purified from the crude venom of Agkistrodon acutus using anionexchange and hydrophobic interaction chromatography. Acurhagin is a monomer with a molecular mass of 51.4 kDa under non-reducing conditions on SDS-PAGE and 48,133 Da by mass spectrometry. Partial amino acid sequence of its metalloproteinase domain is homologous to other high-molecular mass metalloproteinases from snake venoms. It preferentially cleaved Aα. chain of fibrinogen, followed by Bβ chain, while γ chains was minimally affected. Monitored by RP-HPLC, it extensively degraded fibrinogen into various peptide fragments. In aqueous solution, acurhagin autoproteolyzed to a 30 kDa fragment at 37° C. The N-terminal sequence of the 30 kDa fragment of acurhagin showed a high homology to those proteins consisting of disintegrinlike and cysteine-rich domains. Caseinolytic assay showed that the proteinase activity of acurhagin was slightly enhanced by Ca2+ and Mg2+, but completely inhibited by Zn2+. When treated with metal chelators, acurhagin was completely inactivated. Furthermore, acurhagin exerts an inhibitory effect on ADP-induced platelet aggregation of plateletrich plasma in an incubation-time dependent manner. It also impairs collagen- and ristocetin-induced platelet aggregation by cleaving collagen and vWF, respectively.
Experiments and Analyses of Distributed Exhaust Nozzles (2002)

Experimentaland analytical aeroacoustic properties of several distributed exhaust nozzle (DEN) designs are presented.Significant differences between the designs are observed and correlated back to CFD flowfield predictions.Up to 20 dB of noise reduction on a spectral basis and 10 dB on an overall sound pressure level basis are demonstrated from the DEN designs compared to a round reference nozzle.The most successful DEN designs acoustically show a predicted thrust loss of approximately 10% compared to the reference nozzle.Characteristics of the individual mini-jet nozzles that comprise the DEN such as jet-jet shielding and coalescence are shown to play a major role in the noise signature.
The genetics of sports injuries and athletic performance (2019)

Purpose: in the last two decades, several evidences have been provided to support the relationship between single nucleotide polymorphisms and the susceptibility to develop injuries participating in sport and performance related to sports activity. We report up-to-date review of the genetics factors involved in tendon injuries and athletic performance. Methods: we searched PubMed using the terms "sports injuries", "athletic performance" and "genetics" over the period 1990 to the present day. We also included non-English journals. Results: most of the currently established or putative tendinopathy susceptibility loci have been analyzed by candidate gene studies. The genes currently associated with tendon injuries include gene encoding for collagen, matrix metallopeptidase, tenascin and growth factors. Several genes have been related to the physical performance phenotypes affecting endurance capacity and muscle performance. The most studied include ACE and ACTN3 genes. Conclusions: genetics determines the response of an individual to the surrounding environment. Recently, some of the individual genetic variations contributing to the athletic performance and the onset of musculoskeletal injuries, particularly in tendon and ligament tissues, have been identified. However, the identification of the genetic background related to susceptibility to injuries and physical performance of the athletes is challenging yet and further studies must be performed to establish the specific role of each gene and the potential effect of the interaction of these.
Plasma acylcarnitine levels increase with healthy aging (2020)

Acylcarnitines transport fatty acids into mitochondria and are essential for β-oxidation and energy metabolism. Decreased mitochondrial activity results in increased plasma acylcarnitines, and increased acylcarnitines activate proinflammatory signaling and associate with age-related disease. Changes in acylcarnitines associated with healthy aging, however, are not well characterized. In the present study, we examined the associations of plasma acylcarnitines with age (range: 20-90) in 163 healthy, non-diseased individuals from the predictive medicine research cohort (NCT00336570) and tested for gender-specific differences. The results show that long-chain and very long-chain acylcarnitines increased with age, while many odd-chain acylcarnitines decreased with age. Gender-specific differences were observed for several acylcarnitines, e.g., eicosadienoylcarnitine varied with age in males, and hydroxystearoylcarnitine varied in females. Metabolome-wide association study (MWAS) of age-associated acylcarnitines with all untargeted metabolic features showed little overlap between genders. These results show that plasma concentrations of acylcarnitines vary with age and gender in individuals selected for criteria of health. Whether these variations reflect mitochondrial dysfunction with aging, mitochondrial reprogramming in response to chronic environmental exposures, early pre-disease change, or an adaptive response to healthy aging, is unclear. The results highlight a potential utility for untargeted metabolomics research to elucidate gender-specific mechanisms of aging and age-related disease.
Microarthropod contributions to fitness variation in the common moss Ceratodon purpureus (2021)

The evolution of sustained plant-animal interactions depends critically upon genetic variation in the fitness benefits from the interaction. Genetic analyses of such interactions are limited to a few model systems, in part because genetic variation may be absent or the interacting species may be experimentally intractable. Here, we examine the role of sperm-dispersing microarthropods in shaping reproduction and genetic variation in mosses. We established experimental mesocosms with known moss genotypes and inferred the parents of progeny from mesocosms with and without microarthropods, using a pooled sequencing approach. Moss reproductive rates increased fivefold in the presence of microarthropods, relative to control mesocosms. Furthermore, the presence of microarthropods increased the total number of reproducing moss genotypes, and changed the rank-order of fitness of male and female moss genotypes. Interestingly, the genotypes that reproduced most frequently did not produce sporophytes with the most spores, highlighting the challenge of defining fitness in mosses. These results demonstrate that microarthropods provide a fitness benefit for mosses, and highlight the potential for biotic dispersal agents to alter fitness among moss genotypes.
DeepFaceLab: A simple, flexible and extensible face swapping framework. (2020)

DeepFaceLab is an open-source deepfake system created by \textbf{iperov} for face swapping with more than 3,000 forks and 13,000 stars in Github: it provides an imperative and easy-to-use pipeline for people to use with no comprehensive understanding of deep learning framework or with model implementation required, while remains a flexible and loose coupling structure for people who need to strengthen their own pipeline with other features without writing complicated boilerplate code. In this paper, we detail the principles that drive the implementation of DeepFaceLab and introduce the pipeline of it, through which every aspect of the pipeline can be modified painlessly by users to achieve their customization purpose, and it's noteworthy that DeepFaceLab could achieve results with high fidelity and indeed indiscernible by mainstream forgery detection approaches. We demonstrate the advantage of our system through comparing our approach with current prevailing systems. For more information, please visit: this https URL.
Stress-Induced Potentials in Moist Bone in Vitro (1974)

Moist bovine cortical bone specimens were milled to exact dimensions and subjected to bending stress under carefully controlled conditions. The surface under compression was consistently electronegative with respect to that under tension, and potentials up to 7.6 millivolts were recorded. With loads above one kilogram the relationship of peak voltage to load was nearly linear while the relationship of voltage to rate of load was non-linear, the increments in voltage being small beyond a certain rate. Under constant load there was a steady-state potential that was directly proportional to the load but not dependent on the rate of loading. A mathematical equation was derived relating voltage to time, load, and load rate. Although the potentials were clearly stress-induced, and perhaps more accurately strain-related, their origin could not be determined from these studies.
PROCEDURE FOR PREDICTING OCCURRENCE AND SPACING OF THERMAL-SUSCEPTIBILITY CRACKING IN FLEXIBLE PAVEMENTS (1978)

The inability of previous models to accurately predict transverse cracking in pavements in the southwestern United States has led to the laboratory validation of the concept of thermal susceptibility of unstabilized base-course material. Thermal susceptibility is the volumetric contraction of a granular base-course materials when it is frozen. This contraction produces cracking in the base course that is highly dependent on the environment in the general area. This paper presents the results of a study that attempted to quantify this mechanism and to predict crack spacings and the rate of crack appearance. The procedure used involves the combination of linear viscoelasticity and linear visco-elastic fracture mechanics. Crack spacings in the base course are calculated by using the results of a finite-element study of the frozen propertions of the base course. The effect of these cracks (i.e., the rate at which they propagate refelction cracks through the asphalt surface layer) is determined by using a fracture mechanics approach. Intensity factors are calculated for the viscoelastic thermal stress in the asphalt caused by a temperature cycle and for the stress caused by the deformation of a crack in the base course caused by a freeze. The integration of these distributions in the Paris equation for crack growth gives the number of cycles to failure. These calculations were assembled into a computer model that uses daily climatic data and appropriate material properties to calculate the damage caused by daily temperature cycles. The behavior of asphalt and of sulfur asphalt are contrasted when placed over several base-course materials in two different environments, Abiliene and Amarillo, Texas, to illustrate the effects of materials-property variation and environmental influence. This model represents an initial theoretical approach to the problem of combining the effects on flexible pavements of environment and traffic to more accurately predict performance. Implications concerning reflection cracking can be drawn directly from the analysis of the effect of thermal susceptiblity of the base course on the rate of reflection crack appearance. /Author/
Supplementary Figure 1 from <i>In Vitro</i> Sensitivity of CLL Cells to Fludarabine May Be Modulated by the Stimulation of Toll-like Receptors (2023)

&lt;p&gt;PDF file - 2581K, Stratification according to the underlying genetics&lt;/p&gt;
Inhibition of Wee1, AKT, and CDK4 Underlies the Efficacy of the HSP90 Inhibitor XL888 in an In Vivo Model of NRAS -Mutant Melanoma (2013)

The HSP90 inhibitor XL888 is effective at reversing BRAF inhibitor resistance in melanoma, including that mediated through acquired NRAS mutations. The present study has investigated the mechanism of action of XL888 in NRAS-mutant melanoma. Treatment of NRAS-mutant melanoma cell lines with XL888 led to an inhibition of growth, G2-M phase cell-cycle arrest, and the inhibition of cell survival in three-dimensional spheroid and colony formation assays. In vitro, HSP90 inhibition led to the degradation of ARAF, CRAF, Wee1, Chk1, and cdc2 and was associated with decreased mitogen-activated protein kinase (MAPK), AKT, mTOR, and c-jun NH2 kinase (JNK) signaling. Apoptosis induction was associated with increased BIM expression and a decrease in the expression of the prosurvival protein Mcl-1. The critical role of increased BIM and decreased Mcl-1 expression in the survival of NRAS-mutant melanoma cell lines was shown through siRNA knockdown and overexpression studies. In an animal xenograft model of NRAS-mutant melanoma, XL888 treatment led to reduced tumor growth and apoptosis induction. Important differences in the pattern of client degradation were noted between the in vivo and in vitro studies. In vivo, XL888 treatment led to degradation of CDK4 and Wee1 and the inhibition of AKT/S6 signaling with little or no effect observed upon ARAF, CRAF, or MAPK. Blockade of Wee1, using either siRNA knockdown or the inhibitor MK1775, was associated with significant levels of growth inhibition and apoptosis induction. Together, these studies have identified Wee1 as a key target of XL888, suggesting novel therapeutic strategies for NRAS-mutant melanoma.
Supplemental Figure 1 from Exploitation of Castration-Resistant Prostate Cancer Transcription Factor Dependencies by the Novel BET Inhibitor ABBV-075 (2023)

&lt;p&gt;ABBV-075 inhibits AR-dependent transcription and growth.&lt;/p&gt;
HT‐29 and Caco‐2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation (2015)

The NF-κB is a transcription factor which plays a key role in regulating biological processes. In response to signals, NF-κB activation occurs via phosphorylation of its inhibitor, which dissociates from the NF-κB dimer allowing the translocation to the nucleus, inducing gene expression. NF-κB activation has direct screening applications for drug discovery for several therapeutic indications. Thus, pathway-specific reporter cell systems appear as useful tools to screen and unravel the mode of action of probiotics and natural and synthetic compounds. Here, we describe the generation, characterization, and validation of human epithelial reporter cell lines for functional studies of NF-κB activation by different pro- and anti-inflammatory agents. Caco-2 and HT-29 cells were transfected with a pNF-κB-hrGFP plasmid which contains the GFP gene under the control of NF-κB binding elements. Three proinflammatory cytokines (TNF-α, IL-1β, and LPS) were able to activate the reporter systems in a dose-response manner, which corresponds to the activation of the NF-κB signaling pathway. Finally, the reporter cell lines were validated using lactic acid bacteria and a natural compound. We have established robust Caco-2-NF-κB-hrGFP and HT-29-NF-κB-hrGFP reporter cell lines which represent a valuable tool for primary screening and identification of bacterial strains and compounds with a potential therapeutic interest.
Johnny-0, a compliant, force-controlled and interactive humanoid autonomous robot (2012)

Johnny-0, shown in Figure 1, is our new humanoid robot which integrates an expressive face on an orientable head, two arms with 4 degrees of freedom (DOF) each and grippers, mounted on an omnidirectional, non-holonomic mobile platform. Our underlying goal with Johnny-0 is to design a platform capable of natural reciprocal interaction (motion, language, touch, affect) with humans, to address integration issues associated with advanced motion, interaction and cognition capabilities on the same platform, and their use in unconstrained real world conditions. To do so, compliance is a necessity to provide natural and safe interactions.
Robo-Chargers: Optimal Operation and Planning of a Robotic Charging System to Alleviate Overstay (2022)

Charging infrastructure availability is a major concern for plug-in electric vehicle users. Nowadays, the limited public chargers are commonly occupied by vehicles which have already been fully charged. Such phenomenon, known as overstay, hinders other vehicles' accessibility to charging resources. In this paper, we analyze a charging facility innovation to tackle the challenge of overstay, leveraging the idea of Robo-chargers - automated chargers that can rotate in a charging station and proactively plug or unplug plug-in electric vehicles. We formalize an operation model for stations incorporating Fixed-chargers and Robo-chargers. Optimal scheduling can be solved with the recognition of the combinatorial nature of vehicle-charger assignments, charging dynamics, and customer waiting behaviors. Then, with operation model nested, we develop a planning model to guide economical investment on both types of chargers so that the total cost of ownership is minimized. In the planning phase, it further considers charging demand variances and service capacity requirements. In this paper, we provide systematic techno-economical methods to evaluate if introducing Robo-chargers is beneficial given a specific application scenario. Comprehensive sensitivity analysis based on real-world data highlights the advantages of Robo-chargers, especially in a scenario where overstay is severe. Validations also suggest the tractability of operation model and robustness of planning results for real-time application under reasonable model mismatches, uncertainties and disturbances.
Ultrasensitive Label-free MiRNA Sensing Based on a Flexible Graphene Field-Effect Transistor without Functionalization (2020)

A flexible graphene field-effect transistor (Gr-FET) biosensor for ultrasensitive and specific detection of miRNA without labeling and functionalization is reported. The flexible biosensor presents robust performance even after multiple cycles of bending to a cylinder with an 8 mm radius. A DNA probe is designed with partial segment complementary to target miRNA, and immobilized on the graphene surface though π–π stacking interaction. After capture of target miRNA, a Dirac point shift in Gr-FET is induced, which shows a linear relationship with the target miRNA concentration on a semi-log scale. The Gr-FET-based biosensor finishes miRNA detection in 20 min, and is able to achieve a miRNA detection limit as low as 10 fM without any functionalization and labeling. The interaction processes of DNA-graphene and DNA-miRNA are confirmed through surface-enhanced Raman scattering technology. The proposed biosensor will have prospective applications in wearable electronics for health monitoring and disease diagnosis.
Power consumption analysis of Bluetooth Low Energy, ZigBee and ANT sensor nodes in a cyclic sleep scenario (2013)

This paper is intended to guide developers of wireless systems who are puzzled by the vast number of radio configuration parameters and options. We provide experimental data comparing power consumption of Bluetooth Low Energy (BLE), ZigBee and ANT protocols for a cyclic sleep scenario, in which a short-range and low-power wireless sensor node periodically sends a data packet to a remote `hub' with intervening sleep intervals. Devices such as wearable health monitors often use this scenario when interfacing with a mobile phone-based hub. For all measured sleep intervals BLE achieved lower power consumption (10.1 uA, 3.3 V supply at 120 s interval), compared with ZigBee (15.7 uA), and ANT (28.2 uA). Most of the power consumption differences can be attributed to the time taken for a node to connect to the hub after waking up and the use of sleep between individual RF packets. For the three protocols we determined a sleep interval at which the tradeoff between power consumption and data rate is optimized.
Board # 17 :An Educational Kit for Introducing Microfluidics-based Cell Adhesion Assay in Undergraduate Laboratory (Work in Progress) (2018)

in 1996 with a bachelor's degree in precision instruments and a minor in electronics and computer yechnology
Leptin and insulin in young adulthood are associated with weight in infancy (2019)

Low weight in early infancy is a known risk factor for cardio-metabolic syndrome in adult life. However, little is known either about developmental programming in subjects of normal birthweight or about events between the ages which separate early programming and the occurrence of disease at late adulthood. We tested the hypothesis that circulating concentrations of leptin, adiponectin and insulin in young, healthy adults, born with a birth size within the normal range, are influenced by early life growth patterns. In an observational study of 188 healthy volunteers aged 18-25 years (97 males, 91 females) we investigated the association of metabolic function with their birth size, their growth during childhood and their body composition. High plasma leptin in early adulthood, a risk factor for cardio-metabolic syndrome, was associated with low weight at age 2 years (correlation coefficient controlled for adult weight = -0.21, P < 0.01). It was also positively associated with pre-prandial insulin and with HOMA (Homeostasis Model Assessment) insulin resistance. Leptin, leptin-adiponectin ratio and insulin correlated with lean mass, fat mass and percent fat (P < 0.0001). In conclusion, high leptin in early adulthood was associated with both low weight at age 2 years and insulin resistance. We speculate that high leptin is developmentally programmed and can contribute to the association between low weight in early infancy and increased cardio-metabolic risk in adulthood in healthy subjects.
Isoform‐specific mechanisms of a3b4*‐nicotinic acetylcholine receptor modulation by the prototoxin lynx1 (2017)

This study investigates-for the first time to our knowledge-the existence and mechanisms of functional interactions between the endogenous mammalian prototoxin, lynx1, and α3- and β4-subunit-containing human nicotinic acetylcholine receptors (α3β4*-nAChRs). Concatenated gene constructs were used to express precisely defined α3β4*-nAChR isoforms (α3β4)<sub>2</sub>β4-, (α3β4)<sub>2</sub>α3-, (α3β4)<sub>2</sub>α5(398D)-, and (α3β4)<sub>2</sub>α5(398N)-nAChR in <i>Xenopus</i> oocytes. In the presence or absence of lynx1, α3β4*-nAChR agonist responses were recorded by using 2-electrode voltage clamp and single-channel electrophysiology, whereas radioimmunolabeling measured cell-surface expression. Lynx1 reduced (α3β4)<sub>2</sub>β4-nAChR function principally by lowering cell-surface expression, whereas single-channel effects were primarily responsible for reducing (α3β4)<sub>2</sub>α3-nAChR function [decreased unitary conductance (≥50%), altered burst proportions (3-fold reduction in the proportion of long bursts), and enhanced closed dwell times (3- to 6-fold increase)]. Alterations in both cell-surface expression and single-channel properties accounted for the reduction in (α3β4)<sub>2</sub>α5-nAChR function that was mediated by lynx1. No effects were observed when α3β4*-nAChRs were coexpressed with mutated lynx1 (control). Lynx1 is expressed in the habenulopeduncular tract, where α3β4*-α5*-nAChR subtypes are critical contributors to the balance between nicotine aversion and reward. This gives our findings a high likelihood of physiologic significance. The exquisite isoform selectivity of lynx1 interactions provides new insights into the mechanisms and allosteric sites [α(-)-interface containing] by which prototoxins can modulate nAChR function.-George, A. A., Bloy, A., Miwa, J. M., Lindstrom, J. M., Lukas, R. J., Whiteaker, P. Isoform-specific mechanisms of α3β4*-nicotinic acetylcholine receptor modulation by the prototoxin lynx1.