gebhart/patrepeval huggingface dataset:
[Analysis of the secondary structure of urokinase by the circular dichroism method]. (1984)

The secondary structure of urokinase with molecular weight 33 000 dalton was studied by the circular dichroism method. The secondary structure parameters were calculated based on the protein reference CD spectra resulted in the following secondary structure parameters: approximately 30% aminoacid residues constitute the alpha-helical regions, the same amount forms the beta-structure and an essential fractions contributes the beta-turns. Conformational stability of urokinase to alkaline pH (up to 11.6) and high temperature (up to 80 degrees) in 0.1 M phosphate buffer pH 6.6 was found.
The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity (1997)

The mevalonate diphosphate decarboxylase is an enzyme which converts mevalonate diphosphate to isopentenyl diphosphate, the building block of isoprenoids. We used the Saccharomyces cerevisiae temperature-sensitive mutant defective for mevalonate diphosphate decarboxylase previously described (C. Chambon, V. Ladeveve, M. Servouse, L. Blanchard, C. Javelot, B. Vladescu, and F. Karst, Lipids 26:633-636, 1991) to characterize the mutated allele. We showed that a single change in a conserved amino acid accounts for the temperature-sensitive phenotype of the mutant. Complementation experiments were done both in the erg19-mutated background and in a strain in which the ERG19 gene, which was shown to be an essential gene for yeast, was disrupted. Epitope tagging of the wild-type mevalonate diphosphate decarboxylase allowed us to isolate the enzyme in an active form by a versatile one-step immunoprecipitation procedure. Furthermore, during the course of this study, we observed that a high level of expression of the wild-type ERG19 gene led to a lower sterol steady-state accumulation compared to that of a wild-type strain, suggesting that this enzyme may be a key enzyme in mevalonate pathway regulation.
Supplementary Table 1 from LY2109761 Attenuates Radiation-Induced Pulmonary Murine Fibrosis via Reversal of TGF-β and BMP-Associated Proinflammatory and Proangiogenic Signals (2023)

&lt;p&gt;PDF file, 37KB, Regulated GO-terms and Genes.&lt;/p&gt;
Two-dimensional Electronic Spectroscopy of Photosynthetic Light-Harvesting Complexes (2007)

A variety of two-dimensional electronic spectroscopy experiments on natural light-harvesting complexes reveal inter-chromophore coupling and monitor ultrafast dynamics, furthering understanding of the way these proteins function and the underlying design principles of photosynthesis.
Mapping the Cell Binding Site on High Molecular Weight Kininogen Domain 5 (1995)

Investigations mapped the region(s) on the light chain of high molecular weight kininogen (HK) that participates in cell binding. Sequential and overlapping peptides of domain 5 (D5H) were synthesized to determine its cell binding site(s). Three peptides from non-overlapping regions on D5H were found to inhibit biotin-HK binding to endothelial cells. Peptides GKE19 and HNL 21 weakly inhibited biotin-HK binding with IC50 of 792 and 215 microM, respectively. Peptide HKH20 inhibited biotin-HK binding with an IC50 of 0.2 microM. Two peptides, GGH18 and HVL24, which overlapped HKH20, also inhibited biotin-HK binding to endothelial cells with IC50 values of 108 and 0.8 microM, respectively. Biotinylated HKH20 directly bound to endothelial cells. HK and HKH20 bound at or near the same site on endothelial cells because HK inhibited biotin-HKH20 binding (IC50 = 0.2 microM). A polyclonal anti-HKH20 antibody also blocked biotin-HK binding. Peptides HKH20 and HVL24 and anti-HKH20 antibody also inhibited the procoagulant activity of plasma HK. These data indicated that the cell and artificial surface binding sites on D5H overlap. The orientation of HK on endothelial cells may be critical for the assembly and activation of contact system enzymes and the expression of kininogen's anti-thrombin activity.
80-Gb/s optical demultiplexing using modulators (2001)

Optical Time Division Multiplexing (OTDM) is one of the important techniques by which data transmission capacity (aggregate bit rates) can be increased enormously. In this work, we demonstrate an 80 Gb/s time division multiplexing and demultiplexing experiment. By gain switching a DFB laser with 10 GHz RF signal we produced approximately 7 ps FWHM pulses. By using TDM technique we obtained 80 GHz signal. The demultiplexing of this TDM signal is carried out using two cascaded LiNbO<SUB>3</SUB> Mach-Zehnder modulators.
Large area ion beam sputtered dielectric ultrafast mirrors for petawatt laser beamlines (2022)

The latest advances in petawatt laser technology within the ELI Beamlines project have stimulated the development of large surface area dielectrically coated mirrors meeting all demanding requirements for guiding the compressed 30 J, 25 fs HAPLS laser beam at 10 Hz repetition rate and a center wavelength of 810 nm entirely in vacuum. We describe the production and evaluation of Ta<sub>2</sub>O<sub>5</sub>/HfO<sub>2</sub>/SiO<sub>2</sub> ion beam sputtered coated (440 × 290 × 75) mm<sup>3</sup> beam transport mirrors. No crazing was observed after thirty vacuum-air cycles. A laser induced damage threshold of 0.76 J/cm<sup>2</sup> (fluence on mirror surface) was achieved and maintained at high shot rates.
Dual Bone Fixation: A Biomechanical Comparison of 3 Implant Constructs in a Mid‐Diaphyseal Fracture Model of the Feline Radius and Ulna (2016)

DBF significantly increased construct stiffness and strength. Given the high complication rate reported in cat antebrachial fractures when only the radius is stabilized, surgeons should consider DBF.
Total Syntheses of Linear Polythiazole/Oxazole Plantazolicin A and Its Biosynthetic Precursor Plantazolicin B (2014)

Abstract Plantazolicin A, a linear decacyclic natural product, exhibits desirable selective activity against the causative agent of anthrax toxicity. The total synthesis of plantazolicin A and its biosynthetic precursor plantazolicin B was successfully achieved by an efficient, unified, and highly convergent route featuring dicyclizations to form 2,4‐concatenated oxazoles and the mild synthesis of thiazoles from natural amino acids. This report represents the first synthesis of plantazolicin B and includes the first complete characterization data for both natural products.
WHITE PAPER Knowing What We Know: Supporting Knowledge Creation and Sharing in Social Networks (2000)

Abstract Many early knowledge management initiatives focused heavily on informationtechnology and codified knowledge and so missed performance improvementopportunities from interventions targeting knowledge embedded within networks ofemployees. Despite advanced technical solutions employed to manage organizationalknowledge, we continue to find that people are often more reliant on other people thanthey are on databases when seeking answers to unstructured questions. As a result,organizations creating more cohesive networks on knowledge related dimensions arebetter able to collectively solve problems, create new knowledge and transfer explicit andtacit knowledge embodied within employees. The following article reports on a cross-industry research program assessing ways to promote knowledge creation and transfer innetworks of employees. Specifically, we have found four characteristics of relationshipsimportant for knowledge creation in networks: 1) knowing what others know; 2) havingaccess to other people’s thinking; 3) having people be willing to actively engage inproblem solving; and 4) having a safe relationship to promote learning and creativity.Mapping these dimensions in social networks yields targeted social and technicalinterventions managers can employ to improve a network’s ability to create and shareknowledge.
Vision and air flow combine to streamline flying honeybees (2013)

Insects face the challenge of integrating multi-sensory information to control their flight. Here we study a 'streamlining' response in honeybees, whereby honeybees raise their abdomen to reduce drag. We find that this response, which was recently reported to be mediated by optic flow, is also strongly modulated by the presence of air flow simulating a head wind. The Johnston's organs in the antennae were found to play a role in the measurement of the air speed that is used to control the streamlining response. The response to a combination of visual motion and wind is complex and can be explained by a model that incorporates a non-linear combination of the two stimuli. The use of visual and mechanosensory cues increases the strength of the streamlining response when the stimuli are present concurrently. We propose this multisensory integration will make the response more robust to transient disturbances in either modality.
Lightweight Directory Access Protocol (LDAP): Uniform Resource Locator (2006)

This document describes a format for a Lightweight Directory Access
Protocol (LDAP) Uniform Resource Locator (URL). An LDAP URL describes
an LDAP search operation that is used to retrieve information from an
LDAP directory, or, in the context of an LDAP referral or reference,
an LDAP URL describes a service where an LDAP operation may be
progressed. [STANDARDS-TRACK]
Ductile End Connections for Glulam Beams (2008)

Ductility, which is the capability of a structure to deform significantly beyond the elastic limit without brittle failure, is a fundamental property which can be an important requirement in many instances. In particular, the behaviour of structures under seismic actions depends on the capacity to withstand large displacements in the plastic field without reaching the collapse. Wood suffers from the unfavourable material characteristic that exhibits no (tension) and limited (compression) post-elastic deformation capacity. Therefore timber structures rely on steel dowel-type connections to provide ductility and energy dissipation. In general, good performance can be achieved by light timber structures with a large amount of steel connectors, designed to favour steel yielding as the governing mechanism. For heavy timber structures, because of the lower redundancy of the structure, a more “engineered” joint design is needed to ensure a safe performance during seismic events. The research reported in the present paper has been devoted to the investigation of an innovative steel-to-timber joint suitable for different joint configurations within a frame, where a timber element is connected to a steel stub by means of an end-plate and glued-in steel rods. The proposed system has been investigated by means of numerical models and experimental tests, in order to determine strength values and moment-rotation relationships of the joint. Some interesting conclusions can already be drawn from the available experimental tests, suggesting a possible full implementation of such techniques in seismic resistant timber structures, which is the ultimate goal of the research.
Long-circulating PEG-PE micelles co-loaded with paclitaxel and elacridar (GG918) overcome multidrug resistance (2012)

Overexpression of drug efflux pump P-gp is one of the major reasons to cause multidrug resistance (MDR). To overcome P-gp mediated MDR, modulators, so called P-gp inhibitors, can be used to block efflux pump activity. Elacridar is one of the most potent P-gp inhibitors, which can cause irreversible and total P-gp blockage. Elacridar, among with other P-gp inhibitors, can be used in combination with anticancer drugs to enhance the effectiveness of chemotherapy against resistant tumor cells. On the other hand, P-gp is presented in normal tissues, thus non-selective blockage of P-gp can cause undesired side effects. Therefore, it is important to deliver P-gp inhibitor only to the tumor cells (along with anticancer drug) and limit its distribution in the body. In this study, we have developed PEG-PE-based long-circulating ca. 15 nm micelles co-loaded with elacridar and paclitaxel, and investigated their ability to overcome paclitaxel resistance in two cancer cell lines. Vitamin E, a common solubility enhancer for PEG-PE micelles, was found to have a negative effect on both particle size and encapsulation efficiencies. The human MDR1 gene-transfected and thus paclitaxel-resistant MDCKII-MDR1 P-gp overexpressing cells were used for cytotoxicity evaluation. Even though PEG-PE based micelles itself have a potential to enhance the cytotoxicity of paclitaxel, elacridar/paclitaxel-co-loaded micelles demonstrated the highest cytotoxicity compared to both free and micellar paclitaxel. The obtained results suggest that co-loading of paclitaxel and elacridar into micellar drug carriers results in promising preparations capable of overcoming paclitaxel resistance.
Signal mechanisms in photoacoustic remote sensing microscopy(Conference Presentation) (2016)

We recently introduced photoacoustic remote sensing (PARS) microscopy as an all-optical non-contact optical-resolution modality with absorption-based photoacoustic contrast. A pulsed excitation beam is optically focused into a sample then the resulting photoacoustic signal is sensed using a confocal long-coherence probe beam right at the source of the large pressures generated. Several mechanisms are proposed to explain the source of these large signals, including surface-displacements, local refractive-index step-modulation, scatterer displacements, and photothermal mechanisms. We carefully model each of these mechanisms and predict the fraction of modulated light from each. Experimental measurements detect ~0.1% of the incident interrogation light is modulated and this is confirmed with theoretical modulation calculations. We provide experimental evidence that pressure-induced refractive-index step modulation and scatter position modulation may be highly significant modulation mechanisms. We also model theoretical limitations of signal-to-noise and discuss future system optimization opportunities.
Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate (2019)

The Chikungunya virus (CHIKV) belongs to the Alphavirus genus of Togaviridae family and contains a positive-sense single stranded RNA genome. Infection by this virus mainly causes sudden high fever, rashes, headache, and severe joint pain that can last for several months or years. CHIKV, a mosquito-borne arbovirus, is considered a re-emerging pathogen that has become one of the most pressing global health concerns due to a rapid increase in epidemics. Because handling of CHIKV is restricted to Biosafety Level 3 (BSL-3) facilities, the evaluation of prophylactic vaccines or antivirals has been substantially hampered. In this study, we first iden-tified the whole structural polyprotein sequence of a CHIKV strain isolated in South Korea (KNIH/2009/77). Phylogenetic analysis showed that this sequence clustered within the East/ Central/South African CHIKV genotype. Using this sequence information, we constructed a CHIKV-pseudotyped lenti-virus expressing the structural polyprotein of the Korean CHIKV isolate (CHIKVpseudo) and dual reporter genes of green fluorescence protein and luciferase. We then developed a pseudovirus-based neutralization assay (PBNA) using CHIKVpseudo. Results from this assay compared to those from the conventional plaque reduction neutralization test showed that our PBNA was a reliable and rapid method to evaluate the efficacy of neutralizing antibodies. More importantly, the neutralizing activities of human sera from CHIKV-infected individuals were quantitated by PBNA using CHIKVpseudo. Taken together, these results suggest that our PBNA for CHIKV may serve as a useful and safe method for testing the neutralizing activity of antibodies against CHIKV in BSL-2 facilities.
Microwave Irradiation Assisted Preparation of Chitosan Composite Microsphere for Dye Adsorption (2017)

Chitosan-activated carbon composite microspheres were prepared by emulsion cross-linking method and its adsorption properties for methyl orange were studied. Chitosan solution was mixed with activated carbon powder and then chitosan was cross-linked by epichlorohydrin under microwave irradiation. SEM photos show that the composite microspheres have diameters of 200–400 μ m and activated carbon powder dispersed on the surface of composite microsphere. FTIR spectrum indicates chitosan is successfully cross-linked. Microwave irradiation can effectively shorten the cross-linking time. Composite microspheres have enhanced dye adsorption capacity for methyl orange compared to chitosan microspheres. Kinetic studies showed that the adsorption followed a pseudo-second-order model. Isotherm studies show that the isotherm adsorption equilibrium is better described by Freundlich isotherm. Regeneration results show that adsorption capacity of composite microsphere decreased about 5.51% after being reused for three times. These results indicated that chitosan-activated carbon composite microsphere has potential application in the removal of dye from wastewaters.
Supplementary Figure 1 from Vascular Adhesion Protein-1 Enhances Tumor Growth by Supporting Recruitment of Gr-1<sup>+</sup>CD11b<sup>+</sup> Myeloid Cells into Tumors (2023)

Supplementary Figure 1 from Vascular Adhesion Protein-1 Enhances Tumor Growth by Supporting Recruitment of Gr-1&lt;sup&gt;+&lt;/sup&gt;CD11b&lt;sup&gt;+&lt;/sup&gt; Myeloid Cells into Tumors
Kleider machen Leute: Heck‐Reaktion im neuen Gewand (1994)

Abstract Die von Richard F. Heck Ende der sechziger Jahre entdeckte Palladium‐katalysierte Kupplung von Aryl‐ und Alkenylhalogeniden mit Alkenen hat sich nach gelegentlichem Auf‐ und Abschwellen des darauf gerichteten Interesses in den letzten sechs Jahren nachhaltig gemausert. Durch geschickte Auswahl der Substrate und sorgfältige Anpassung der Reaktionsbedingungen gelingen beeindruckende Sequenzen auch unterschiedlicher Reaktionstypen nicht nur nacheinander, sondern vielfach in einem einzigen Verfahrensschritt. Die mittlerweile etablierte Heck‐Reaktion – und eine Reihe mit ihr mechanistisch verwandter Palladium‐katalysierter Umwandlungen an Aren‐, Alken‐ und Alkinderivaten – bietet ungezählte Möglichkeiten, elegant und hochkonvergent komplexe Moleküle aufzubauen; dabei bereiten Sauerstoff‐ und Stickstoffatome (mit Einschränkungen auch Schwefel‐ und Phosphoratome) in den Reaktionen keine Probleme. Das Spektrum der neueren Erfolge beginnt mit den chemo‐ und regioselektiven Einfachkupplungen hochfunktionalisierter Substrate mit unsymmetrisch mehrfach substituierten Reaktionspartnern. Es reicht allerdings viel weiter über Kaskadenreaktionen mit Knüpfung von drei, vier, fünf oder gar acht neuen CC‐Bindungen unter Bildung von oligofunktionellen und oligocyclischen Produkten von beeindruckender Molekülkomplexität bis hin zum enantioselektiven Aufbau von anspruchsvollen Naturstoffmolekülen mit quartären stereogenen Zentren, wie die Beispiele Crinan, Picrotoxinin, Morphin und viele mehr belegen. Zweifellos läßt sich schon heute die Heck‐Reaktion aus dem Methodenarsenal der präparativen Organischen Chemie nicht mehr wegdenken; abzuwarten bleibt lediglich, wann sie Einzug in ein industrielles Produktionsverfahren halten wird.
GEDANKENSTEUERUNG DER GREIFFUNKTION EINES TETRAPLEGIKERS: I. FUNKTIONELLE ELEKTROSTIMULATION (FES) (2003)

Article GEDANKENSTEUERUNG DER GREIFFUNKTION EINES TETRAPLEGIKERS: I. FUNKTIONELLE ELEKTROSTIMULATION (FES) was published on January 1, 2003 in the journal Biomedical Engineering / Biomedizinische Technik (volume 48, issue s1).
An approach for T-learning content generation based on a social media environment (2012)

The T-Learning paradigm facilitates to the viewer/learner to take an active and leading role in his/her teaching-learning process through the digital interactive television. In this sense, quality educational contents and simple interactive mechanisms with them are required, but there aren't strategies that facilitate the composition of educational material and procure continuity of education across platforms. To solve this lack, a model for generating educational content for cross-platform is presented in this article. This article propose a social media environment where their users can search, share and argue video format resources available on the Web and then combine them with interactive mechanisms to generate interactive multimedia educational content. A real blended learning experience was made to evaluate this proposal.
Experimental Endophthalmitis Treated With an Implantable Osmotic Minipump (1979)

A rabbit model of endophthalmitis was used to test the efficacy of an osmotic minipump delivery system as compared to standard intravitreal injection of the antibiotic, Staphylococcus aureus was inoculated into the vitreous cavity, and elimination of microorganisms was compared with use of intravitreal administration of 100 micrograms of gentamicin sulfate by either single pars plana injection or the continuous four-day administration of drugs to the vitreous via pars plana tubing connected to the minipump implanted subcutaneously near the rabbit's ear.
The 5'-triphosphates of the (-) and (+) enantiomers of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolane-5-yl]cytosine equally inhibit human immunodeficiency virus type 1 reverse transcriptase (1993)

The (-) enantiomer of cis-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolane-5-yl]cytosine is a potent inhibitor of human immunodeficiency virus type 1 in vitro. The 5'-triphosphates of the (-) and (+) enantiomers equally inhibited the production of full-length minus-strand DNA in an endogenous reverse transcriptase reaction, each competitively inhibited DNA synthesis, and each was used as a chain-terminating substrate.
The influence of storing alginate impressions sprayed with disinfectant on dimensional accuracy and deformation of maxillary edentulous stone models (2010)

This study investigated the effects of storing impressions for 3 hours after spraying them with a disinfectant solution on dimensional change and deformation of maxillary edentulous stone models. Three brands of alginate impression materials, characterized by a small degree of contraction in 100% relative humidity, were used. The spray disinfectants used were 1% sodium hypochlorite solution and 2% glutaraldehyde solution. A stone model taken from an impression that had not been sprayed or stored was prepared as a control. The results indicated that the differences in dimensional change between the control and disinfected stone models were less than 24 mum, and that no deformation was observed in the stone models.
Performance Enhancement in 5G Cellular Networks Using Priorities in Network Slicing (2019)

Network Slicing is one of the key features of the new 5G cellular network communication, it proposes the division of one physical network into multiple virtual networks to achieve specific goals such as security, flexibility and control over the network that will provide logical isolation in the devices, services and core networks set up for different characteristics and different types of services. Our proposed work uses end-to-end network slicing concept in 5G networks to solve the key issue of isolating the slices via prioritizing them in order to increase performance and decrease latency for high priority applications. Simulation results using NS-3 network simulator prove our claims and show enhancements in latency and performance.