gebhart/patrepeval huggingface dataset:
Unmasking of Deuterium Kinetic Isotope Effects on the Methane Monooxygenase Compound Q Reaction by Site-Directed Mutagenesis of Component B (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTUnmasking of Deuterium Kinetic Isotope Effects on the Methane Monooxygenase Compound Q Reaction by Site-Directed Mutagenesis of Component BBrian J. Brazeau, Bradley J. Wallar, and John D. LipscombView Author Information Department of Biochemistry, Molecular Biology, and Biophysics and Center for Metals in Biocatalysis University of Minnesota, Minneapolis, Minnesota 55455 Cite this: J. Am. Chem. Soc. 2001, 123, 42, 10421–10422Publication Date (Web):September 28, 2001Publication History Received16 July 2001Published online28 September 2001Published inissue 1 October 2001https://pubs.acs.org/doi/10.1021/ja016632ihttps://doi.org/10.1021/ja016632irapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views566Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Hydrocarbons,Isotope effects,Kinetic parameters,Reaction mechanisms,Surface interactions Get e-Alerts
Genetic Music: When Genes Code for Melodies Instead of Proteins (2020)

In the present work we present a methodology for teaching the basis of the genetic code through music composition, with the aim to combine science and arts learning. The project was carried out by 155 students, the so-called MARGA Consortium, with ages comprised between 10 and 17 years from different public schools located in the Principality of Asturias, Spain. The different groups generated 8 different music works using a short genetic sequence obtained from the human notch1 gene, receptor of mutations leading to chronic lymphocytic leukemia.
Application of engineered chimeric antibodies to the calibration of human antibody standards. (1991)

Immunoassay quantitation of human antibody depends on the availability of reference sera that have been characterized in terms of their antibody content. This report expands upon the concept of "cross-calibration", using reference (dose-response) curves that have been generated with heterologous antibody as a means of calibrating prospective human antibody standards. Engineered human-mouse chimeric antibody, with a single human isotype and specificity, is used to construct the heterologous dose-response curve from which test antibody response data are interpolated. Transfer experiments are used to define and correct for the percentage of the total chimeric antibody that is immunoreactive and bound to solid phase antigen. The chimeric antibody-based calibration procedure is illustrated using H. influenzae Type B as the model antigen and an engineered chimeric IgG1 antibody that has a known mass per volume content and specificity for the hapten, 4-hydroxy-3-nitro-phenylacetyl (NP).
Renal Injury and Recovery in Partial Ureteric Obstruction (1989)

Partial ureteric obstruction was produced by insertion of an obstructing stent into the left ureter of 21 mongrel dogs and the duration of obstruction was varied as follows: Group A (n = 7) for 60 days; Group B (n = 7) for 28 days; Group C (n = 7) for 14 days. Intrapelvic pressure monitoring confirmed that obstruction had been produced by stent insertion and relieved by reimplantation of the left ureter following the designated obstruction period. The extent of recovery of renal function was assessed by creatinine clearance, with group C maintaining normal function, group B recovering 31% and group A recovering 8% of control function. Methyl-methacrylate extrusion casts of the renal microvasculature were made in group B and C kidneys and studied by scanning electron microscopy. During obstruction there was evidence of arteriolar constriction. These changes provide a morphological basis for the renal functional alterations and support the concept that post-obstructive renal failure is a vascular injury.
Supplementary Table 3 from Tumor Genetic Analyses of Patients with Metastatic Melanoma Treated with the BRAF Inhibitor Dabrafenib (GSK2118436) (2023)

&lt;p&gt;Supplementary Table 3 - PDF file 47K, Gene specific list used for correlation, with aggregate percentages of gains and losses in the archival/pre-treatment samples and PFS analysis&lt;/p&gt;
Trans-signaling mediates some key actions of interleukin-6 in the central nervous system (117.6) (2011)

Abstract IL-6 which is implicated in the pathogenesis of immunoinflammatory disorders of the CNS, signals via binding to either the membrane bound IL-6Rα (classical signaling) or soluble (s)IL-6Rα (trans-signaling) that then form a complex with gp130 to activate the JAK/STAT and SHP2/MAPK pathways. Here we determined the relative importance of classical- versus trans-signaling in mediating IL-6 actions in the CNS. Bigenic mice were generated with CNS-restricted, astrocyte-targeted production of IL-6 and the specific inhibitor of IL-6 trans-signaling, sgp130-Fc (termed GFAP-IL6/sgp130 mice). Transgene-encoded IL-6 mRNA levels were similar in the brain of GFAP-IL6 and GFAP-IL6/sgp130 mice. However, GFAP-IL6/sgp130 mice had decreased pY-STAT3 in the brain due to a marked reduction in the total number of pY-STAT3-positive cells and the absence of detectable pY-STAT3 in specific cell types. These changes in pY-STAT3 were associated with disproportionate reductions in various inflammatory markers as well as reduced vascular pathology and blood-brain barrier leakage. Degenerative changes in the cerebellum characteristic of GFAP-IL6 mice were almost absent in GFAP-IL6/sgp130 mice. The findings suggest that in the CNS: (i) sgp130-Fc is able to block IL-6 trans-signaling, (ii) trans-signaling is important for IL-6 cellular communication with selective cellular targets and, (iii) the blocking of trans-signaling may prove to be an effective means for alleviating the detrimental effects of IL-6.
Haptics Assisted Training (HAT) System for children's handwriting (2013)

We present Haptics Assisted Training (HAT) System, a force feedback workstation for transferring and improving handwriting skill. The HAT system is the first practical handwriting training system whose usability was tested both in typical and special education classrooms of a local school district. Simulating the role of occupational therapists (OT), the HAT system guides the user's hand along the sequence of strokes of the reference handwriting recorded by an expert or teacher. Since the handwriting data is formatted as a set of ordered paths, it can teach any form of pen strokes, such as words, shapes, and even drawings. Training tasks are presented as a 3D game which captures children's attention and more effective visual motor integration (VMI) is anticipated than traditional paper materials. In this paper we describe the design and implementation of the prototype. We also report a comparative study on handwriting performance of twenty participants grouped into four categories by education types (special vs. typical) and haptic devices (Phantom Omni™ vs. Novint Falcon™). As a result, we observed that the children quickly adjusted themselves to the system, and they sustained engagement at the word-writing tasks without any intervention. Results also showed improvements in tracing precision on the HAT system over time. Details of the results and lessons learned from the study are discussed.
Automatic methods for determining nitrate and nitrite in water and soil extracts (1970)

Automatic methods for determining nitrate and nitrite in fresh and sea water and in soil extracts with the AutoAnalyzer are described, together with details of the analytical systems. The methods are based on a modification of the manual procedure by Wood, Armstrong and Richards, which consists in reduction of nitrate with copperised cadmium and, with the nitrite thus produced, diazotisation of sulphanilamide, the product being coupled with N-1-naphthylethylenediamine to form a highly coloured azo dye, which is measured at 520 nm. The methods are capable of analysing twenty samples per hour.
An Intramolecular Interaction between Src Homology 3 Domain and Guanylate Kinase-Like Domain Required for Channel Clustering by Postsynaptic Density-95/SAP90 (2000)

Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membrane-associated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. MAGUK proteins contain PDZ, Src homology 3 (SH3), and guanylate kinase (GK)-like domains, all of which can function as sites for specific protein-protein interactions. We report here a direct protein-protein interaction between the SH3 domain and the GK region in the PSD-95 family of MAGUKs. The SH3 domain of the PSD-95 family appears to have an atypical binding specificity, because the classical SH3 binding (-P-X-X-P-) motif is absent from the GK domain. Although SH3-GK binding can occur in either an intramolecular or intermolecular manner, the intramolecular mode is preferred, possibly because of additional tertiary interactions available when the SH3 and GK domains are adjacent in the same polypeptide. Mutations disrupting the intramolecular SH3-GK interaction do not interfere with PSD-95 association with the K(+) channel Kv1.4 or with the GK domain-binding protein GKAP. The same mutations, however, inhibit the clustering of Kv1.4 by PSD-95, suggesting that the intramolecular SH3-GK interaction may modulate the clustering activity of PSD-95.
CONTRIBUTION OF AERODYNAMICS TO FUEL ECONOMY IMPROVEMENTS IN FUTURE PASSENGER CARS (1980)

The influence of aerodynamic drag on the fuel economy of passenger cars is analyzed and compared to other parameters, such as weight and engine size. It is shown that for cars marketed today, more than a 30% difference in drag exists between poorly designed and efficiently designed cars, resulting in differences of more than 12% in fuel economy. A research program was designed to show how the aerodynamic drag of future passenger cars can be reduced. It is concluded that aerodynamics alone can contribute a 10% to 15% fuel economy improvement in future passenger cars, and that when aerodynamics is properly matched to vehicle weight and engine size reductions, its contribution is even greater. It is shown that drag reduction can be achieved with body shapes not too different from those of today's cars, without loss of passenger comfort, and within the scope of current legal restrictions, but with some increase in cost.
Three-dimensional hydrogen bonding between Landers and planar molecules facilitated by electrostatic interactions with Ni adatoms (2018)

Using a combination of UHV-STM and molecular mechanics calculations, we investigate the surface self-assembly of a complex multi-component metal-molecule system with synergistic non-covalent interactions. Hydrogen bonding between three-dimensional Lander-DAT molecules and planar PTCDI molecules, adsorbed closer to the surface, is found to be facilitated by electrostatic interactions between co-adsorbed Ni adatoms and the flexible molecular DAT groups.
Innovation in metabolomics to improve personalized healthcare (2015)

Metabolomics is the systemic study of all small molecules (metabolites) and their concentration as affected by pathological and physiological alterations or environmental or other factors. Metabolic alterations represent a "window" on the complex interactions between genetic expression, enzyme activity, and metabolic reactions. Techniques, including nuclear magnetic resonance spectroscopy, mass spectrometry, Fourier-transform infrared, and Raman spectroscopy, have led to significant advances in metabolomics. The field is shifting from feasibility studies to biological and clinical applications. Fields of application range from cancer biology to stem cell research and assessment of xenobiotics and drugs in tissues and single cells. Cross-validation across high-throughput platforms has allowed findings from expression profiling to be confirmed with metabolomics. Specific genetic alterations appear to drive unique metabolic programs. These, in turn, can be used as biomarkers of genetic subtypes of prostate cancer or as discovery tools for therapeutic targeting of metabolic enzymes. Thus, metabolites in blood may serve as biomarkers of tumor state, including inferring driving oncogenes. Novel applications such as these suggest that metabolic profiling may be utilized in refining personalized medicine.
An in vitro method for testing synergistic action of parasiticides using malachite green and formalin as a model system (1979)

Abstract. An in vitro technique was developed to screen drugs for toxicity to laboratory cultured protozoans. Drugs were diluted using standard microtitration equipment. An easily cultured protozoan, Tetrahymena pyriformis , was added to the dilutions of drugs and viability was checked daily for 10 days. Drugs used as a model system to test the technique were formalin and malachite green. Effective concentrations of formalin determined by this in vitro technique were close to recommended therapeutic treatment levels for Ichthyophthirius . Malachite green was effective at concentrations in excess of recommended therapeutic treatment levels for Ichthyophthirius . The synergistic effect of formalin and malachite green was demonstrated. This technique can he of use in determining effectiveness of new drugs as parasiticides.
More the Merrier: Towards Multi-Emotion and Intensity Controllable Response Generation (2021)

The focus on conversational systems has recently shifted towards creating engaging agents by inculcating emotions into them. Human emotions are highly complex as humans can express multiple emotions with varying intensity in a single utterance, whereas the conversational agents convey only one emotion in their responses. To infuse human-like behaviour in the agents, we introduce the task of multi-emotion controllable response generation with the ability to express different emotions with varying levels of intensity in an open-domain dialogue system. We introduce a Multiple Emotion Intensity aware Multi-party Dialogue (MEIMD) dataset having 34k conversations taken from 8 different TV Series. We finally propose a Multiple Emotion with Intensity-based Dialogue Generation (MEI-DG) framework. The system employs two novel mechanisms: viz. (i) determining the trade-off between the emotion and generic words, while focusing on the intensity of the desired emotions; and (ii) computing the amount of emotion left to be expressed, thereby regulating the generation accordingly. The detailed evaluation shows that our proposed approach attains superior performance compared to the baseline models.
The diabetic pregnancy and offspring adiposity in infancy and childhood: a meta-analysis (2014)

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
Monolithic quartz structure vibrating beam accelerometer (VBA) (2002)

This paper describes an accelerometer for which the vibrating beam sensor, the vibration isolation system, the proof mass, the proof mass suspension system, and the mounting means are all machined from a single rectangular quartz crystal blank. As such, this sensor represents a significant advancement in combining the sensor art with the quartz crystal resonator art. The design uses the well-known force-frequency modulation effect of a vibrating beam in axial tension or compression.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Classification of Time-Series Data Using a Generative/Discriminative Hybrid (2004)

Classification of time-series data using discriminative models such as SVMs is very hard due to the variable length of this type of data. On the other hand generative models such as HMMs have become the standard tool for modeling time-series data due to their efficiency. This paper proposes a general generative/discriminative hybrid that uses HMMs to map the variable length time-series data into a fixed p-dimensional vector that can be easily classified using any discriminative model. The hybrid system was tested on the MNIST database for unconstrained handwritten numerals and has achieved an improvement of 1.23% (on the test set) over traditional 2D discrete HMMs.
Factors Influencing Elastic Stresses in Double Cantilever Beam Specimens (1988)

An elastic stress analysis was conducted for a double cantilever beam (DCB) specimen using finite-element methods. The purpose of this study was to identify the important parameters that influence stresses ahead of the delamination front. The study focused on an aluminum DCB specimen, typical of adhesively-bonded joints, and on a graphite/epoxy specimen representing a co-cured composite. Opening mode σy stresses ahead of the crack tip were calculated and compared with those for a monolithic reference specimen. Beyond the singularity-dominated region very near the crack tip, the σy distribution was elevated compared to the monolithic case. Both the adhesive thickness and the adherend transverse (thickness-direction) stiffness were found to influence the elevation of σy. In contrast, the thickness of the adherend and its longitudinal stiffness had very little effect on this stress distribution. Estimates for adhesive yielding beyond the aluminum DCB crack tip showed that both the area and height of the plastic zone increased to a peak value for increasing adhesive thicknesses. Results from this study should provide insight for comparing data from different DCB specimens and for designing new DCB specimens.
Balanced distributed coding of omnidirectional images (2007)

This paper presents a distributed coding scheme for the representation of 3D scenes captured by stereo omni-directional cameras. We consider a scenario where images captured from two different viewpoints are encoded independently, with a balanced rate distribution among the different cameras. The distributed coding is built on multiresolution representation and partitioning of the visual information in each camera. The encoder transmits one partition after entropy coding, as well as the syndrome bits resulting from the channel encoding of the other partition. The decoder exploits the intra-view correlation and attempts to reconstruct the source image by combination of the entropy-coded partition and the syndrome information. At the same time, it exploits the inter-view correlation using motion estimation between images from different cameras. Experiments demonstrate that the distributed coding solution performs better than a scheme where images are handled independently, and that the coding rate stays balanced between encoders.
The delivery of surgical cleft care in the United Kingdom. (2001)

A national survey of cleft services has demonstrated a need for reorganisation. This is now in process and once established will require continual monitoring and assessment.
Photochemical reaction of Si-substituted ethynylsilanes with 1,2-ethanedithiol (1985)

The authors investigate the chief products of the photochemical reactions of Si-substituted ethynylsilanes with 1,2,-ethanedithiol at 60-70 C. It is found that the chief products are 2-triorganylsilyl-substituted 1,4-dithiacyclopentanes and 1,4-dithiacyclohexanes. On lowering the temperature to -30 C, formation of bis (triorganylsilyl)-substituted 1,4,7,10-tetrathiacyclododecanes occurs along with the abo ve-mentioned five- and six-membered heterocycles.
Faculty Opinions recommendation of Effects of estradiol with micronized progesterone or medroxyprogesterone acetate on risk markers for breast cancer in postmenopausal monkeys. (2006)

Read the full review for this Faculty Opinions recommended article: Effects of estradiol with micronized progesterone or medroxyprogesterone acetate on risk markers for breast cancer in postmenopausal monkeys.
A novel technique for detecting single nucleotide polymorphisms by analyzing consumed allele-specific primers (2001)

We present a simple and rapid polymerase chain reaction (PCR)-based technique, termed consumed allele-specific primer analysis (CASPA), as a new strategy for single nucleotide polymorphism (SNP) analysis. The method involves the use of labeled allele-specific primers, differing in length, with several noncomplementary nucleotides added in the 5'-terminal region. After PCR amplification, the amounts of the remaining primers not incorporated into the PCR products are determined. Thus, nucleotide substitutions are identified by measuring the consumption of primers. In this study, the CASPA method was successfully applied to ABO genotyping. In the present method, the allele-specific primer only anneals with the target polymorphic site on the DNA, so it is not necessary to analyze the PCR products. Therefore, this method is only little affected by modification of the PCR products. The CASPA method is expected to be a useful tool for typing of SNPs.
Three-dimensional organization of primary lens fiber cells. (2000)

The three-dimensional cellular organization of GFP-expressing cells is consistent with previous analyses of fiber cell morphology in the embryonic nucleus of adult human and bovine lenses. The present data confirm that the disorganized appearance of primary fiber cells observed in adult lenses is largely a reflection of developmental processes rather than a consequence of aging.
Simultaneous application of differential servo-oscillometry and volume-clamp plethysmography for continuous non-invasive recording of the finger blood pressure response to a hand postural change (2006)

Continuous blood pressure recordings obtained by the volume-clamp method (Finapres 2300 BP monitor, Ohmeda, USA) were compared with measurements made by using the modified oscillometric method (UT9201 beat-to-beat monitor, University of Tartu, Estonia) in normal subjects during a hand postural change. Changes in the local blood pressure were induced in 11 healthy volunteers by passive elevation of the hand by 40 cm and lowering it again after one minute. We did not reveal a statistically significant difference between the group-averaged responses of the oscillometric finger mean blood pressure measurement device and those of Finapres during this test. The difference UT9201 minus Finapres equalled -1.3 +/- 3.1 mm Hg (p = 0.15). Blood pressure responses were similarly tracked by both beat-to-beat finger blood pressure monitors despite the considerable variability in the shape of the subjects' individual responses.