gebhart/patrepeval huggingface dataset:
Abstract WP115: Cilostazol Addition to Aspirin Does Not Alter the Short-Term Neurological Outcome in Each Clinical Subtype of Acute Stroke (2020)

Hypothesis: Our previous study, ADS reported that dual antiplatelet therapy (DAPT) using cilostazol and aspirin did not reduce the rate of short-term neurological worsening in non-cardioembolic stroke patients. The aim of the present study is to investigate 1) whether the impact of cilostazol addition to aspirin differ among each stroke subtype, and 2) factors associated with neurological deterioration and/or stroke recurrence in order to find therapeutic target. Methods: This is a retrospective analysis using the ADS databank. Neurological worsening and the rates of stroke recurrence within 14 day of onset were evaluated. Stroke subtype included large-artery atherosclerosis (LAA), lacunae infarct (LI), branch atheromatous disease (BAD), other, and undetermined. Results: Data on 1,160 patients (773 [67%] men; median age, 69 [61-77] years, NIHSS score was 2 [1-4]) were analyzed. At discharge, 167 (14%) were diagnoses as having LAA; LI, 532 (46%); BAD, 173 (15%); other, 132 (11%); and undetermined, 156 (14%). Neurological deterioration and/or recurrence were seen in 130 (11%) patients, and the rates were not different between patients treated with DAPT and aspirin in any stroke subtypes: LAA, 19% (DAPT) vs. 11% (aspirin alone), (p=0.185); LI, 4% vs. 3% (p=0.645); BAD, 33% vs.34%, (p=0.872), other, 8% vs.14% (p=0.272); undetermined, 13% vs. 8% (p=0.301). When we evaluated factors related to the deterioration/recurrence, age (p&lt;0.001), NIHSS score (p&lt;0.001), systolic and diastolic blood pressures (p&lt;0.001, and 0.025), whiter matter change (p=0.002), large infarcts &gt;1.5cm (p&lt;0.001), and intracranial stenosis/occlusion (p&lt;0.001) were found. Multivariate regression analysis revealed older age (p=0.003), systolic blood pressure (p=0.013), larger infarct (p=0.001), intracranial stenosis/occlusion (p&lt;0.035) were the independent factors associated with neurological deterioration/stroke recurrence. Conclusions: Dual antiplatelet therapy using cilostazol and aspirin does not reduce the rate of short-term neurological worsening in each clinical stroke subtype. Improvement of hyperacute therapy targeting the elder patients with elevated blood pressure, large infarct and intracranial stenosis/occlusion should be required.
Molecular Phylogeny and Surface Morphology of Colpodella edax (Alveolata): Insights into the Phagotrophic Ancestry of Apicomplexans (2003)

The molecular phylogeny of colpodellids provides a framework for inferences about the earliest stages in apicomplexan evolution and the characteristics of the last common ancestor of apicomplexans and dinoflagellates. We extended this research by presenting phylogenetic analyses of small subunit rRNA gene sequences from Colpodella edax and three unidentified eukaryotes published from molecular phylogenetic surveys of anoxic environments. Phylogenetic analyses consistently showed C. edax and the environmental sequences nested within a colpodellid clade, which formed the sister group to (eu)apicomplexans. We also presented surface details of C. edax using scanning electron microscopy in order to supplement previous ultrastructural investigations of this species using transmission electron microscopy and to provide morphological context for interpreting environmental sequences. The microscopical data confirmed a sparse distribution of micropores, an amphiesma consisting of small polygonal alveoli, flagellar hairs on the anterior flagellum, and a rostrum molded by the underlying (open-sided) conoid. Three flagella were present in some individuals, a peculiar feature also found in the microgametes of some apicomplexans.
Are Fetal-Type Posterior Cerebral Arteries Associated With an Increased Risk of Posterior Communicating Artery Aneurysms? (2018)

PCoAAs were associated with a higher prevalence of ipsilateral F-PCAs. This variant was associated with larger sizes of the aneurysm necks but was not associated with the sizes of the aneurysm domes or with their rupture statuses.
Local Organizing Committee (2013)

Deadline was 1.12.2012. Abstracts will be published online in Brain Behavior and Immunity (IF= 4.720, Editor-in-Chief: Keith W. Kelley), the official journal of the PsychoNeuroImmunology Research Society. Please find the abstracts via URL: http://dx.doi.org/10.1016/j.bbi.2013.01.074 Educational Short Course The GEBIN offers an Educational Course organized by Prof. Dr. Adriana del Rey, Marburg. It is intended to teach basic elements of Behavior-Neuro-Endocrino-Immunology to students. Time: March 20, 2013, 14:00 – 18:00, and March 21, 2013, 9:00 – 13:00 Venue: Groser Dollingersaal im Alten Rathaus, Rathausplatz, Regensburg For further details of the course and for registration, please visit the GEBIN homepage: www.gebin.org or contact Adriana del Rey, Dept. of Physiology, Professor at the University of Marburg, e-mail: delrey@mailer.uni-marburg.de
High Breakdown Field Dielectric Elastomer Actuators Using Encapsulated Polyaniline as High Dielectric Constant Filler (2010)

Abstract A novel method allowing rapid production of reliable composites with increased dielectric constant and high dielectric strength for dielectric elastomer actuators (DEA) is reported. The promising approach using composites of conductive particles and insulating polymers generally suffers from low breakdown fields when applied to DEA devices. The present publication shows how to overcome this deficiency by using conductive polyaniline (PANI) particles encapsulated into an insulating polymer shell prior to dispersion. PANI particles are encapsulated using miniemulsion polymerization (MP) of divinylbenzene (DVB). The encapsulation process is scaled up to approximately 20 g particles per batch. The resulting particles are used as high dielectric constant ( ϵ′ ) fillers. Composites in a polydimethylsiloxane (PDMS) matrix are prepared and the resulting films characterized by dielectric spectroscopy and tensile tests, and evaluated in electromechanical actuators. The composite films show a more than threefold increase in ϵ′ , breakdown field strengths above 50 V μm −1 , and increased strain at break. These novel materials allow tuning the actuation strain or stress output and have potential as materials for energy harvesting.
The compositional dependence of thermodynamic interactions in blends of model polyolefins (1994)

We have investigated the thermodynamic interactions as functions of component volume fraction φ and temperature T in six binary polymer blend systems. The components in all cases were model polyolefins, made by saturating the double bonds of nearly monodisperse polydienes. Small-angle neutron scattering measurements for single-phase melts, analyzed with the incompressible random phase approximation, were expressed in terms of the Flory–Huggins interaction parameter χ for 27 °C≤T≤167 °C and 0.1≤φ≤0.9. In most systems we found a characteristic upturn in χ at the composition extremes that diminished with increasing temperature. We also found that the component chain dimensions were unaffected by the strength of the interactions. Several theoretical attempts to explain the dependence of χ on component volume fraction were examined. Most were qualitatively inconsistent with the results, and none were fully satisfactory.
Pectoral Fin Locomotion in Fishes: Testing Drag-based Models Using Three-dimensional Kinematics (1996)

Paired fin propulsion in fishes has classically been divided into two categories which represent biomechanical extremes in the use of appendages for propulsion: lift-based and drag-based mechanisms of thrust production. Theoretical models predict that fishes using drag-based propulsion should have wedge-shaped fins with relatively blunt distal edges, a fin beat cycle that is oriented along the anteroposterior (x) axis, feathering of the fin to reduce drag during the protraction phase, and maximal fin area during the retraction phase as the fin sweeps posteriorly perpendicular to the body. In this paper we use a three-dimensional analysis of pectoral fin propulsion in the largemouth bass, Micropterus salmoides, to (1) evaluate the extent to which bass pectoral fin kinematics fit predictions of drag-based propulsion, and (2)demonstrate the complexity of fin movement when the traditional two-dimensional analysis is extended into three dimensions. We attached small markers to visualize the diaphanous distal fin edge, and we videotaped lateral and ventral views from which we could measure x, y, and z coordinates from the fin and body. We divided the fin into two triangular elements for which we calculated planar (three-dimensional) angles relative to each of three reference planes (XY, YZ and XZ) during the fin beat cycle. We show how angles of attack based only on two-dimensional data may result in gross errors that severely compromise understanding of the mechanics and hydrodynamics of pectoral propulsion. Furthermore, three-dimensional analysis revealed that bass fin kinematics are much more complex than expected on a rowing model of drag-based propulsion, and that the pectoral fins may produce drag-based thrust even during protraction. Three dimensional kinematic data are critical to understanding the hydrodynamics of aquatic animal propulsion. Such data are a necessary foundation for reconstructing patterns of movement, modeling (both theoretical and empirical), and for assessing the extent to which motion is under active control or a passive consequence of fluid resistance.
The VLSI design and implementation of the array processors of a multilayer vision system architecture (2002)

This paper describes the VLSI design and simulation of the lower layer processors of the KYDON vision system. KYDON is a completely autonomous, hierarchical, multilayered image understanding system. The VLSI design of the individual components as well as the timing simulation results of the processor array have been presented. The system runs at 50 MHz and promises a high processing rate of 300 image frames/sec.
Evidence supporting distinct functions of three cytosolic glutamine synthetases and two NADH-glutamate synthases in rice (2014)

The functions of the three isoenzymes of cytosolic glutamine synthetase (GS1;1, GS1;2, and GS1;3) and two NADH-glutamate synthases (NADH-GOGAT1 and NADH-GOGAT2) in rice (Oryza sativa L.) were characterized using a reverse genetics approach and spatial expression of the corresponding genes. OsGS1;2 and OsNADH-GOGAT1 were mainly expressed in surface cells of rice roots in an NH4 (+)-dependent manner. Disruption of either gene by the insertion of endogenous retrotransposon Tos17 caused reduction in active tiller number and hence panicle number at harvest. Re-introduction of OsGS1;2 cDNA under the control of its own promoter into the knockout mutants successfully restored panicle number to wild-type levels. These results indicate that GS1;2 and NADH-GOGAT1 are important in the primary assimilation of NH4 (+) taken up by rice roots. OsGS1;1 and OsNADH-GOGAT2 were mainly expressed in vascular tissues of mature leaf blades. OsGS1;1 mutants showed severe reduction in growth rate and grain filling, whereas OsNADH-GOGAT2 mutants had marked reduction in spikelet number per panicle. Complementation of phenotypes seen in the OsGS1;1 mutant was successfully observed when OsGS1;1 was re-introduced. Thus, these two enzymes could be important in remobilization of nitrogen during natural senescence. Metabolite profiling data showed a crucial role of GS1;1 in coordinating metabolic balance in rice. Expression of OsGS1:3 was spikelet-specific, indicating that it is probably important in grain ripening and/or germination. Thus, these isoenzymes seem to possess distinct and non-overlapping functions and none was able to compensate for the individual function of another.
Push–pull extrusion: A new approach for the solid‐state deformation illustrated with high density polyethylene (1981)

Abstract The crystalline state deformation of high density polyethylene has been examined at an extrusion draw ratio of 30 over a range of temperatures and pressures. The experiments involve combined pushing (extrusion) and pulling through a conical die. The pressure dependence of the extrusion rate through conical dies is given by a logarithmic relation and the temperature dependence by an activation energy of ∼95 kcal/mole. An equation established for the total applied force linearly relates the pulling and extrusion pressure components and represents a force balance at the die entrance and exit. Steady‐state extrusion, with or without pulling, was feasible in a pressure range beyond which fractures occurred owing to strain rate and shear or tensile failure. Under some circumstances the extrusion rate was increased by ten times. The mechanical properties and mode of deformation were not affected by pull load and fibers with a tensile modulus of 55 GPa were produced at T &lt; 110°C.
Efficient Array-Based Identification of Novel Cardiac Genes through Differentiation of Mouse ESCs (2008)

Remarkably, although cardiac disease accounts for the largest proportion of adult mortality and morbidity in the industrialized world, the genetic programs controlling early cardiogenesis are largely incompletely understood. To better understand this process, we set out to identify genes whose expression is enriched within early cardiac fated populations, obtaining the transcriptional signatures of mouse embryonic stem cells (mESCs) at defined intervals during their differentiation along a cardiac path. We compared the RNA profiles of cardiac precursors cells (CPCs) with time-matched non-CPCs and undifferentiated mESCs, using a transgenic mESC line harboring an Nkx2-5 cardiac-specific regulatory sequence driving green fluorescent protein (GFP) to facilitate selection of CPCs. We identify 176 transcripts that are significantly elevated in their abundance within CPCs compared with other assayed populations, predicting that they will likely play a role in cardiogenesis. Of note, approximately 24% (43/176) of the cardiogenic candidate transcripts have known roles in cardiac function or development. Importantly, we evaluated the biological relevance of a significant subset 31/133 (23%) of the remaining candidate genes by in situ hybridization at multiple time points during development (embryonic day, E7.5-9.5) and report that all were expressed in key cardiac structures during cardiogenesis. Furthermore 9/31, of which many were previously uncharacterized, were detected as early as the formation of the cardiac crescent. These data demonstrate the potential power of integrating genomic approaches with mESC differentiation to illuminate developmental processes, and provides a valuable resource that may be mined to further elucidate the genetic programs underlying cardiogenesis.
On-orbit damage assessment for large space structures (1988)

The need to monitor the dynamic characteristics of large structural systems for purposes of assessing the potential degradation of structural properties has been established. This paper develops a theory for assessing the occurrence, location, and extent of potential damage using on-orbit response measurements. Feasibility of the method is demonstrated using a simple structural system as an example.
Qualification des détecteurs de fuite frigorigènes - Qualification of refrigerant leak detectors (2009)

CD-Rom
Quantitative Growth of Naegleria in Axenic Culture (1973)

A strain of Naegleria gruberi, isolated from a Vero cell culture and designated TS-1, was axenically cultivated in monolayer and mass aerating suspension culture. Cultural conditions for constant growth parameters and high-exponential cell densities were defined. Serum or other supplemented fractions were found essential in both Trypticase-yeast extract-glucose (TYG) and Casitone (CAS)-based media. Monolayer cultures grown in the CAS medium required lower levels of serum to reach maximum stationary densities of amoebae than cultures grown in the TYG medium. Heat-killed (121 C, 10 min) whole cell and cell lysate bacterial fractions were capable of replacing the serum in both the TYG and CAS media. Heat-killed bacterial fractions provided the same levels of growth as attained with serum in TYG medium, whereas the bacterial lysate supported only minimal growth in the same medium. In the CAS medium, both bacterial fractions resulted in the same level of growth which was equal to that obtained in reduced serum content. Strain TS-1 was established in suspension culture with the CAS medium used in monolayer culture. The addition of sheep red blood cells (RBC) or RBC lysate greatly enhanced growth responses. Further modifications resulted in a final medium for suspension culture consisting of Casitone-yeast extract-glucose-vitamin base, supplemented with serum and RBC lysate. This medium supported growth with a mean generation time of 9 h at 30 C and a stationary phase yield of greater than 5 x 10(6) amoebae per ml.
Sound absorption and transmission through flexible micro-perforated structures (2013)

This work presents a theoretical and experimental study on sound absorption and transmission through structures made up of single and multiple-layer Micro-Perforated Panels (MPPs). As they contribute to improve acoustical comfort, speech intelligibility and comply with lightweight, transparency and fibreless requirements, increasing applications are found in architectural acoustics or in the aeronautic and surface transport industries. A fully coupled modal approach is proposed to calculate the absorption coefficient and the Transmission Loss of finite-sized layouts made up of multiple flexible MPPs separated by air gaps. Validation results are obtained for single and double-layer thin MPPs against the transfer matrix approach and against measurements performed in a standing wave tube and in an anechoic chamber. Analytical approximations are derived from coupled-mode analysis for the Helmholtz-type and structural resonance frequencies of a single layer MPP structure together with relationships on the air-frame relative velocity over the MPP surface at these resonances. Principled guidelines are provided for enhancing both the sound absorption and transmission properties of mutiple-layer MPP structures through suitable setting of the design parameters.
WHIM syndrome myelokathexis reproduced in the NOD/SCID mouse xenotransplant model engrafted with healthy human stem cells transduced with C-terminus–truncated CXCR4 (2006)

WHIM(warts, hypogammaglobulinemia, recurrent bacterial infection, and myelokathexis) syndrome is a rare immunodeficiency caused in many cases by autosomal dominant C-terminal truncation mutations in the chemokine receptor CXCR4. A prominent and unexplained feature of WHIM is myelokathexis (hypercellularity with apoptosis of mature myeloid cells in bone marrow and neutropenia). We transduced healthy human CD34(+) peripheral blood-mobilized stem cells (PBSCs) with retrovirus vector encoding wild-type (wt) CXCR4 or WHIM-type mutated CXCR4 and studied these cells ex vivo in culture and after engraftment in a nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse xenograft model. Neither wt CXCR4 nor mutated CXCR4 transgene expression itself enhanced apoptosis of neutrophils arising in transduced PBSC cultures even with stimulation by a CXCR4 agonist, stromal cell-derived factor-1 (SDF-1 [CXCL12]). Excess wt CXCR4 expression by transduced human PBSCs enhanced marrow engraftment, but did not affect bone marrow (BM) apoptosis or the release of transduced leukocytes into PB. However, mutated CXCR4 transgene expression further enhanced BM engraftment, but was associated with a significant increase in apoptosis of transduced cells in BM and reduced release of transduced leukocytes into PB. We conclude that increased apoptosis of mature myeloid cells in WHIM is secondary to a failure of marrow release and progression to normal myeloid cell senescence, and not a direct effect of activation of mutated CXCR4.
Biochemical and genomic analysis of the denitrification pathway within the genus Neisseria (2009)

Since Neisseria gonorrhoeae and Neisseria meningitidis are obligate human pathogens, a comparison with commensal species of the same genus could reveal differences important in pathogenesis. The recent completion of commensal Neisseria genome draft assemblies allowed us to perform a comparison of the genes involved in the catalysis, assembly and regulation of the denitrification pathway, which has been implicated in the virulence of several bacteria. All species contained a highly conserved nitric oxide reductase (NorB) and a nitrite reductase (AniA or NirK) that was highly conserved in the catalytic but divergent in the N-terminal lipid modification and C-terminal glycosylation domains. Only Neisseria mucosa contained a nitrate reductase (Nar), and only Neisseria lactamica, Neisseria cinerea, Neisseria subflava, Neisseria flavescens and Neisseria sicca contained a nitrous oxide reductase (Nos) complex. The regulators of the denitrification genes, FNR, NarQP and NsrR, were highly conserved, except for the GAF domain of NarQ. Biochemical examination of laboratory strains revealed that all of the neisserial species tested except N. mucosa had a two- to fourfold lower nitrite reductase activity than N. gonorrhoeae, while N. meningitidis and most of the commensal Neisseria species had a two- to fourfold higher nitric oxide (NO) reductase activity. For N. meningitidis and most of the commensal Neisseria, there was a greater than fourfold reduction in the NO steady-state level in the presence of nitrite as compared with N. gonorrhoeae. All of the species tested generated an NO steady-state level in the presence of an NO donor that was similar to that of N. gonorrhoeae. The greatest difference between the Neisseria species was the lack of a functional Nos system in the pathogenic species N. gonorrhoeae and N. meningitidis.
ChemInform Abstract: Convenient Synthesis of 3,5‐Biscarbamoyl‐pyridine Derivatives. (2012)

Abstract The described procedure provides a facile work‐up.
ERα17p, an ERα P295-T311 fragment, modifies the migration of breast cancer cells, through actin cytoskeleton rearrangements (2011)

Recently, our knowledge on estrogen receptor alpha (ERα) functions and fate has progressed: ERα enters in repeated transcription-modulating cycles (nucleus/cytoplasm/membrane trafficking processes and proteasomal degradation) that are governed by specific protein-protein interactions. Receptor fragments, especially those resulting from the proteolysis of its ligand binding domain, as well as corresponding synthetic peptides, have been studied with respect to their estrogenic/antiestrogenic potency. A peptide, corresponding to the human ERα P(295) -T(311) sequence (ERα17p) has been shown to alter breast cancer cell fate, triggering proliferation, or apoptosis. The aim of this work was to explore the effect of ERα17p on breast cancer cell migration and actin cytoskeleton dynamics and further analyze the mechanism of its membrane action. We show that ERα17p increases (MCF-7 and SK-BR-3 cells) or decreases (T47D and MDA-MB-231 cells) migration of breast cancer cells, in an ERα-independent manner, by mechanism(s) depending on Rho/ROCK and PI3K/Akt signaling pathways. Moreover, the peptide enhances the association of both estrogens and androgens to membranes and modifies cell migration, induced by E(2) -BSA. Additionally, initial evidence of a possible agonistic action of the peptide on GPR30 is also provided. ERα17p can be considered as a cell migration-modulator and could therefore constitute a therapeutic challenge, even in anti-estrogen-resistant tumors.