gebhart/patrepeval huggingface dataset:
Breast stem cells spontaneously fuse with breast cancer cells: Impacts on cancer stem cell formation? (2008)

AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA

5007 

Recent discoveries give rise to the theory that fusion events between stem cells and tumor cells could act as malignant and crucial originators in Cancer Stem Cell (CSC) generation. By now CSCs are known as cancer initiating cells with characteristic features of stem cells like self-renewing, differentiation, tissue restoration at low cell numbers and drug resistance. This rare population of cancer initiating cells attracts attention as a target in cancer therapies. M13SV1-EGFP-Neo breast stem cells were co-cultured with HS578T-Hyg and MDA-MB-435-Hyg, respectively, breast cancer cells. After 24h cell were cultured under selective conditions (+ G418, + Hyg) and cell clones emerged from spontaneous fusion events were isolated and cultivated separately. Short tandem repeat analysis of hybrid cells showed an overlap of the parental alleles indicating that hybrid cells have been originated from real cell fusion events. Characterization of hybrid cells revealed variations among them as well as in comparison to their parental cell lines. Proliferation studies revealed higher proliferation rates of distinct hybrid cell clones of up to 1.5-fold in comparison to the appropriate parental breast cancer cells and up to 10-fold as compared to the breast stem cell line, respectively. RealTime-PCR-based gene expression pattern analyses, which have also been validated by Western-Blot analysis, revealed multiple up- and down-regulation of cancer and drug metabolism genes in hybrid cell clones as compared to their appropriate parental relatives. For instance, distinct M13HS and M13MDA hybrid cells, derived from M13SV1-EGFP-Neo and HS578T-Hyg and M13SV1-EGFP-Neo and MDA-MB-435-Hyg fusion events, respectively, showed an up-regulation of drug resistance transporters such as ABCC6, ABCG2 and Major Vault Protein as well as EGFR up-regulation. We further observed androgen receptor up-regulation, but estrogen receptor-1 down-regulation in distinct M13SV1 and M13MDA hybrid cell lines suggesting that fusion of breast stem cells with breast cancer cells might cause a switch to an estrogen-independent tumor growth. FACS analysis of adhesion molecules revealed differential expression of β1-integrin expression levels in M13MDA hybrid cells, which correlated with differential adhesive properties of these cells. Interestingly, cell migration studies using the 3D-collagen matrix migration assay revealed that both the diverse hybrid cell clones and the parental cells possess similar migratory activities and susceptibilities toward stimulatory agents. Nevertheless, the finding that breast stem cell/ breast cancer cell hybrids exhibit a higher proliferatory activity and increased expression levels of drug resistance transporters indicate that such fusion events might contribute to tumor progression and possible to the evolution of a CSC phenotype.
Guiding security analysis through visualization (2011)

We present a multiple views visualization for the security data in the VAST 2010 Mini Challenge 2. The visualization is used to monitor log event activity on the network log data included in the challenge. Interactions are provided that allow analysts to investigate suspicious activity and escalate events as needed. Additionally, a database application is used to allow SQL queries for more detailed investigation.
Local Principal Component Analysis for Fault Detection in Air-Condensed Water Chillers (2018)

Water chillers play a crucial role in HVAC systems. Faulty operations of chillers can lead to energy wastage, system unreliability and shorter equipment life. Due to the intrinsic complexity of these systems, which are nonlinear with interrelated parameters, and since data regarding unforeseen phenomena and abnormalities are not usually available for air-conditioning installations, the development of fault detection algorithms is a burdensome task. In this paper, a data-driven approach is used in order to develop a fault detection methodology that makes no use of a priori knowledge about abnormal phenomena. In particular, we exploit a local Principal Component Analysis to handle nonlinear cases and to accent novelties with respect to non-faulty operations variability. The performance of the proposed approach is assessed by using a synthetic dataset which is related to an air-condensed water chiller in both fault-free and faulty conditions.
Effectiveness and safety of a home-based cardiac rehabilitation programme of mixed surveillance in patients with ischemic heart disease at moderate cardiovascular risk: A randomised, controlled clinical trial (2017)

Retrospectively registered NCT02796404 (May 23, 2016).
Development of water gas shift/membrane hybrid system for precombustion CO2 capture in a coal gasification process (2011)

In this study, a 2l/min water gas shift/membrane hybrid system for pre-combustion CO2 capture has been developed. To control the concentration of major components such as H2, CO, and CO2, MFCs were used in experimental apparatus. The gas concentration in these experiments was equal with syngas concentration from dry coal gasifiers (H2: 25–35, CO: 60–65, CO2: 5–15 vol%). The operation conditions of WGS/membrane hybrid system were 200–400 °C, 1–15bar. Steam/Carbon ratios were between 2.0 and 5.0. To separate hydrogen from mi xed gas stream, the palladium membrane will be adopted. As steam/carbon ratio increased, the conversion in the HTS reactor increased from 85% to 91% at the condition of 350 °C, 1,000 ml/min, CO: 65, H2: 30, CO2: 5%. However the conversion decreased with increasing of gas flow. In WGS experi ments, the conversion reached 99.5% at the condition of 1,000 ml/min and CO: 65, H2: 30, CO2: 5%. In the experiments of WGS with membrane reactor, the gas concentration before membrane reactor was H2: 56.28, CO2: 43.48, CO: 0.24%. The gas concentration of retentate flow was H2: 35.74, CO2: 63.27, CO: 0.99%.
Identification of Defective TSVs in Pre-Bond Testing of 3D ICs (2011)

Pre-bond testing of TSVs has been identified as a major challenge for yield assurance in 3D ICs. We present a defective-TSV localization technique based on pre-bond probing of a network of TSVs. We describe an efficient algorithm for designing parallel TSV test sessions such that test time is reduced and a given number of faulty TSVs within the TSV network can be uniquely identified. Optimization results for various networks of TSVs highlight significant reduction in test time using the proposed method.
Flexible Fingers Based on Shape Memory Alloy Actuated Modules (2019)

To meet the needs of present-day robotics, a family of gripping flexible fingers has been designed. Each of them consists of a number of independent and flexible modules that can be assembled in different configurations. Each module consists of a body with a flexible central rod and three longitudinally positioned shape memory alloy (SMA) wires. When heated by the Joule effect, one to two SMA wires shorten, allowing the module to bend. The return to undeformed conditions is achieved in calm air and is guaranteed by the elastic bias force exerted by the central rod. This article presents the basic concept of the module and a simple mathematical model for the design of the device. Experimental tests were carried out on three prototypes with bodies made of different materials. The results of these tests confirm the need to reduce the antagonistic action of the inactive SMA wires and led to the realization of a fourth prototype equipped with an additional SMA wire-driven locking/unlocking device for these wires. The preliminary results of this last prototype are encouraging.
Involvement of Extracellular Signal-Regulated Kinases 1/2 in Cardiac Hypertrophy and Cell Death (2002)

In response to pathophysiological stress, the adult heart undergoes hypertrophic enlargement characterized by an increase in the cross-sectional area of individual myofibers. Although cardiac hypertrophy is initially a compensatory response, sustained hypertrophy is a leading predictor for the development of heart failure. At the molecular level, disease-related stimuli invoke endocrine, paracrine, and autocrine regulatory circuits, which directly influence cardiomyocyte hypertrophy, in part, through membrane bound G protein-coupled receptors and receptor tyrosine kinases. These membrane receptors activate intermediate signal transduction pathways within the cytoplasm such as mitogen-activated protein kinases (MAPKs), protein kinase C (PKC), and calcineurin, which directly modify transcriptional regulatory factors promoting alterations in cardiac gene expression. This review will weigh an increasing body of literature implicating the intermediate signaling pathway consisting of MEK1 and extracellular signal-regulated kinases (ERK1/2) as important regulators of cardiac hypertrophy and myocyte survival. The MEK1-ERK1/2 pathway likely occupies a central regulatory position in the signaling hierarchy of a cardiac myocyte given its unique ability to respond to virtually every characterized hypertrophic agonist and stress stimuli examined to date and based on its ability to promote myocyte growth in vitro and in vivo.
Comparative analysis of whole blood lysis methods for flow cytometry (1997)

We performed a parallel evaluation of six whole blood lysis methods comparing light scatter and quantitative fluorescence intensity based on quantitative flow cytometry, of selected lymphocyte subsets and CD34+ cells. Leukocytes prepared with FACS Lysing Solution (BDIS), Immunolyse (Coulter) and Optilyse B (Immunotech) consistently gave lower forward scatter values than those prepared with ACK (BioWhitaker), Ortho-mune (Ortho) and ImmunoPrep (Coulter). Debris, defined as CD45 negative events with the threshold off, accounted approximately 80% of all events with ACK and Ortho-mune. The other lysing methods consistently yielded less debris (approximately 50%) with Immunolyse generating only approximately 16% debris. Optilyse and FACS lyse consistently displayed the lowest percentage of lymphoid cells (CD45+/CD14-) in the three part differential. The percentage of CD3+, CD20+, CD5+, and CD16/CD56+ cells was consistent with all methods but CD4 and CD8 determinants showed inconsistent variation with ACK and Ortho-mune. In addition, the fluorescence intensity of CD14 PE and CD8 PE staining was markedly decreased on cells prepared with ImmunoPrep. Finally, the clearest separation of CD34+ cells was observed with ACK and Ortho-mune. Our data demonstrate that the method used for red cell lysis can have definite impact on immunophenotyping and selected methods appear to be more suitable for specific applications.
SOS induction in Escherichia coli and Salmonella mutagenicity: a comparison using 330 compounds (1994)

To examine the concordance of two microbial genotoxicity short-term assays, 330 experimental results for the SOS chromotest using tester strain Escherichia coli PQ37 were compared with the results of the Salmonella/mammalian microsome mutagenicity assay with Salmonella typhimurium TA97, TA98, TA100, TA102, TA104, TA1535, TA1537 and/or TA1538. With respect to qualitative features, the concordance between SOS chromotest and Salmonella mutagenicity test results was 86.4% (sensitivity, 78.6%; specificity, 100%; chi 2 = 188.6). None of the non-mutagens (N = 120) were able to induce the SOS system. Additionally, 45 of the 210 S.typhimurium mutagens (21.5%) did not induce the SOS repair system. On closer examination, the majority of these 45 compounds (84%) were mutagens with activities between 0.001 and 10 rev/nmol. Even though the experimental protocols of both systems were not standardized, the correlation coefficient for the experimental results of the two test systems was 0.7 for the 330 chemicals. Except for aliphatic epoxides (r = 0.47), the mutagenicity/SOS induction correlations for congeneric data sets (polycyclic aromatic hydrocarbons, nitroarenes, nitroarenofurans, mycotins) were even better (r = 0.72-0.95). Additionally, computer automated structure evaluation (CASE) analyses of the nature of the structural determinants associated with each endpoint indicate extensive homologies. The data can be taken to indicate that the two phenomena reflect common mechanisms of action.
Output characteristics of a discharge-pumped F2 laser (157 nm) with an injection-seeded unstable resonator (1993)

To improve beam quality, an injection-seeded unstable resonator was applied to a discharge-pumped F2 laser, which is normally dominated by amplified spontaneous emission with a large beam divergence when using a normal stable cavity, due to the laser’s extremely high gain (a small-signal gain-length product ∼29). The time-dependent injection-seeding efficiency was obtained for various injection timings by measuring a ratio of the output laser intensity with the same polarization as the seed laser. Correlation of the injection-seeding efficiency with the temporal evolution of the laser-beam divergence was also studied. Both the time-dependent beam divergence and the injection-seeding efficiency showed degradation at a later part of the laser pulse.
Density-driven damage mechanics (D3-M) model for concrete I: mechanical damage (2020)

Damage in concrete has been modelled using various approaches such as fracture mechanics, continuum damage mechanics and failure envelope theories. This study proposes a new approach to model the initiation of damage in concrete that addresses some limitations associated with the existing approaches. The proposed approach defines damage in terms of changes in the density of the material at the microscopic level, where such changes are induced by mechanical loading. The suggested approach is used to simulate the response of 2D concrete bodies to uni-axial tension and uni-axial compression. The simulation results indicate that the proposed model, by means of a single constitutive function, is able to correctly predict failure patterns and aptly capture the damage mechanisms under both uni-axial tension and uni-axial compression loadings using only the information related to the microstructure, the density field and the stiffness field. As a continuation, in Part II, the ability of the D3-M approach to model fully coupled chemo-mechanical damage in concrete using a single constitutive equation will be demonstrated.
Heart Valve Biomechanics: The Frontiers of Modeling Modalities and the Expansive Capabilities of Ex Vivo Heart Simulation (2021)

The field of heart valve biomechanics is a rapidly expanding, highly clinically relevant area of research. While most valvular pathologies are rooted in biomechanical changes, the technologies for studying these pathologies and identifying treatments have largely been limited. Nonetheless, significant advancements are underway to better understand the biomechanics of heart valves, pathologies, and interventional therapeutics, and these advancements have largely been driven by crucial in silico, ex vivo , and in vivo modeling technologies. These modalities represent cutting-edge abilities for generating novel insights regarding native, disease, and repair physiologies, and each has unique advantages and limitations for advancing study in this field. In particular, novel ex vivo modeling technologies represent an especially promising class of translatable research that leverages the advantages from both in silico and in vivo modeling to provide deep quantitative and qualitative insights on valvular biomechanics. The frontiers of this work are being discovered by innovative research groups that have used creative, interdisciplinary approaches toward recapitulating in vivo physiology, changing the landscape of clinical understanding and practice for cardiovascular surgery and medicine.
The Safety of Sublingual Immunotherapy With One or Multiple Pollen Allergens in Children (2009)

Agostinis F, Foglia C, Landi M, et al. Allergy. 2008;63(12):1637–1639

PURPOSE OF THE STUDY. To evaluate the rate and type of adverse events experienced by children receiving sublingual immunotherapy (SLIT) for pollen allergy with either single or multiple allergen extracts.

STUDY POPULATION. Prospective postmarketing survey of 433 children receiving SLIT for respiratory allergies attributable to pollen.

METHODS. Consecutive children with respiratory allergies attributable to pollens who were receiving SLIT with multiple or single allergens were enrolled. Parents recorded adverse events (eye symptoms, rhinitis/ear …
Role of P-selectin and PSGL-1 in coagulation and thrombosis (2004)

P-selectin and PSGL-1 are cell adhesion molecules, regulating the initial interactions between leukocytes and the blood vessel wall as well as between activated platelets and leukocytes. P-selectin is expressed on activated endothelial cells and platelets, while its major ligand PSGL-1 is expressed on leukocytes. Multiple studies have shown that these adhesion molecules are required for the normal recruitment of leukocytes during an inflammatory reaction. More recently, these adhesion molecules have been implicated in recruitment of leukocytes and leukocyte microparticles to thrombi. The P-selectin- and PSGL-1-dependent delivery of circulating microparticles to thrombi appears to be important for normal tissue factor accumulation and fibrin generation in thrombi.
Expression of catalytically active human multifunctional glycogen‐debranching enzyme and lysosomal acid alpha‐glucosidase in insect cells (1996)

Glycogen debranching enzyme and acid alpha-glucosdase are responsible for glycogen degradation in human. The formal enzyme is a multifunctional enzyme with two independent catalytic activities occurring on a single polypeptide, while the latter is a lysosomal enzyme which matures through extensive glycosylation and phosphorylation and proteolytic processing. Deficiency of glycogen debranching enzyme and acid alpha-glucosidase cause glycogen storage disease type III and II, respectively. Baculovirus/insect expression system was used to produce both GDE and GAA. Both enzymes were found to be catalytically and antigenically active. The majority of recombinant GDE is present in the medium (70%). Uptake experiment indicated that GAA produced in the insect cells could not be absorbed into the GSD type II patient fibroblasts through mannose-6-phosphate receptor mediated endocytosis. Uptake experiment combined with immunoblot analysis indicated there are differences in the posttranslational modification and processing between insect cells and mammalian cells.
N-Carboxybiotin formation by pyruvate carboxylase: the stereochemical consequence at phosphorus (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTN-Carboxybiotin formation by pyruvate carboxylase: the stereochemical consequence at phosphorusDavid E. Hansen and Jeremy R. KnowlesCite this: J. Am. Chem. Soc. 1985, 107, 26, 8304–8305Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/ja00312a105https://doi.org/10.1021/ja00312a105research-articleACS PublicationsRequest reuse permissionsArticle Views121Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Separation of 1,3-Propanediol from Aqueous Solutions Using Pervaporation through an X-type Zeolite Membrane (2001)

1,3-Propanediol was separated from aqueous solutions by pervaporation through an X-type zeolite membrane on a γ-Al2O3 support. Binary, ternary, and quaternary mixtures and a cell-free fermentation broth were used. At 308 K, the total fluxes for the quaternary solution and the fermentation broth were 2.1 and 1.2 kg/(m2 h), respectively. Pervaporation fluxes of all components increased with temperature, but the water flux increased at the fastest rate. From 308 to 328 K, the 1,3-propanediol/glycerol selectivity for the quaternary model solution increased from 59 to 67; it increased from 61 to 110 for the broth. The 1,3-propanediol/glucose selectivity increased from 1900 to 2200. The 1,3-propanediol/glycerol selectivity was mainly controlled by preferential adsorption, and the 1,3-propanediol/glucose selectivity was mainly controlled by differences in diffusion rates. The membrane was stable during the pervaporation of the quaternary mixture for at least one week. In contrast, after 60 h, the broth started to foul the membrane, and the 1,3-propanediol permeate concentration decreased. The membrane was regenerated by calcination at 653 K for 4 h.
Similarity and stability of face network across populations and throughout adolescence and adulthood (2021)

The ability to extract cues from faces is fundamental for social animals, including humans. An individual's profile of functional connectivity across a face network can be shaped by common organizing principles, stable individual traits, and time-varying mental states. In the present study, we used data obtained with functional magnetic resonance imaging in two cohorts, IMAGEN (N = 534) and ALSPAC (N = 465), to investigate - both at group and individual levels - the consistency of the regional profile of functional connectivity across populations (IMAGEN, ALSPAC) and time (Visits 1 to 3 in IMAGEN; age 14 to 22 years). At the group level, we found a robust canonical profile of connectivity both across populations and time. At the individual level, connectivity profiles deviated from the canonical profile, and the magnitude of this deviation related to the presence of psychopathology. These findings suggest that the brain processes faces in a highly stereotypical manner, and that the deviations from this normative pattern may be related to the risk of mental illness.
FACTORS AFFECTING THE THYROID GLAND UPTAKE OF I131OF THERANA CATESBEIANATADPOLE1 (1959)

Various factors affecting thyroid gland uptakes of I131 of the tadpole have been studied. The species of Rana catesbeiana was used since they were widely available the year around in the larval form. All observations, except for one study, were made on non-metamorphosing tadpoles fasted 7 to 14 days. Temperature had a marked effect on the uptake and must be critically controlled to compare responses of these poi kilo thermic animals. The responses varied during the months of the year being greatest in April and least in October, November and December. When single injections of TSH and I131 were given the greatest difference in the mean uptake between the stimulated and unstimulated tadpoles was observed when TSH was given 68 hoursand I131 24 hours before sacrifice. Twentyfive /xg. triiodothjTonine placed in the tadpole water bathof approximately 1200 ml. suppressed the 24 hour uptake by 50%. This effect occurred within 24 hours and lasted at least 96 hours. The response to TSH was not decreased by the tri...