gebhart/patrepeval huggingface dataset:
The splicing modulator sulfonamide indisulam reduces AR-V7 in prostate cancer cells (2020)

Alternative splicing of the androgen receptor (AR) is frequently observed in castration resistant prostate cancer (CRPC). One AR isoform, the AR-V7 splice variant, is a constitutively active transcription factor which lacks a ligand binding domain and is therefore undruggable. AR-V7 expression correlates with resistance to androgen receptor signaling inhibitors (ARSi) and poor clinical prognoses. The occurrence of the AR-V7 splice variant is driven by alternative splicing of AR pre-mRNA by the spliceosome, however the mechanistic details are poorly understood. We demonstrate that the splicing factor RBM39 is critical for alternative splicing of the AR-V7 splice variant mRNA transcripts from AR pre-mRNA, and that the anti-cancer drug, indisulam, reduces AR-V7 mRNA levels by degrading RBM39. We report that indisulam effectively reduces AR-V7 in in vitro and in vivo models.
Permutation fixed points with application to estimation of minimum distance of turbo codes (2000)

We present a systematic technique for obtaining all the input sequences that are mapped by a given permutation either to themselves or to shifted versions of themselves (generically called permutation fixed points). Such sequences or their subsets, represent the primary candidates for examination in connection with obtaining estimates of the minimum distance of parallel concatenated codes, specially for interleaver lengths for which the determination of the actual minimum distance may be very difficult. Subsequently, we present a new class of permutations that nearly achieve the lower bound on the number of possible fixed points associated with a given permutation of prime length p. Preliminary experimental evidence suggests that certain permutations of this class lead to turbo codes with large minimum distances fur short interleaver lengths.
Formation constants and metal-to-ligand ratios for tris(hydroxymethyl)aminomethane-metal complexes (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation constants and metal-to-ligand ratios for tris(hydroxymethyl)aminomethane-metal complexesP. J. Brignac and Celine. MoCite this: Anal. Chem. 1975, 47, 8, 1465–1466Publication Date (Print):July 1, 1975Publication History Published online1 May 2002Published inissue 1 July 1975https://doi.org/10.1021/ac60358a026RIGHTS & PERMISSIONSArticle Views476Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (239 KB) Get e-Alerts Get e-Alerts
Flexible Gd2O2S:Tb scintillators pixelated with polyethylene microstructures for digital x-ray image sensors (2008)

Flexible scintillators for digital x-ray image sensors were designed, fabricated and characterized. In these scintillaotrs, terbium-doped gadolinium oxysulfide (Gd2O2S:Tb) scintillator pixels were embedded into a polyethylene (PE) substrate. To evaluate the difference in the spatial resolution according to the pixel size, we designed three scintillators with pixels of different pitch sizes: 50 µm pitch size (P50), 100 µm pitch size (P100) and 200 µm pitch size (P200). Because of the high flexibility and good formability, polyethylene was used as the substrate of the scintillator. To fabricate nickel micromolds with high-aspect-ratio microstructures, two microfabrication techniques were employed: silicon dry-etching using a deep reactive ion etching (DRIE) process and nickel electroforming. The pixelated PE microstructures were fabricated by a hot embossing process. Because the solution-type Gd2O2S:Tb precursor can be handled at room temperature, Gd2O2S:Tb was used as the scintillator material. The measured sensitivities of the P50 and P100 models were, respectively, about 65% and 97% of that of the P200 model. The lower sensitivity values of the models with a small pitch size were due to two factors, such as the different pixel heights and the different fill factors. Because a scintillator with a small pixel size has a low fill factor, the sensitivity of the scintillator decreases as the pixel size decreases. The fill factors of the P50, P100 and P200 models were 36%, 49% and 56.25%, respectively. On the other hand, the spatial resolution of the scintillator increases as the pixel size decreases. Therefore, P50 gave the best spatial resolution among the designed models. The spatial frequency at 10% of the modulation transfer function (MTF) with P50 was 13.5 mm−1, while that with P200 was 10.0 mm−1. The resolution pattern and the tooth x-ray images obtained from a scintillator with a smaller pixel size was also clearer than that obtained from a scintillator with a larger pixel size. PE-based flexible Gd2O2S:Tb scintillators can be utilized directly in flexible x-ray image sensors.
Lack of Suppression of Insulin Secretion by Hyperinsulinemia in a Patient with an Insulinoma* (1986)

The regulation of insulin secretion in patients with insulinoma is known to be abnormal. For example, physiological and pharmacological stimuli often fail to stimulate insulin in such patients. Recently, insulin has been found to inhibit its own secretion in normal subjects. To determine if insulin has this effect in patients with insulinoma, we infused insulin at rates of 1 and 10 mU/kg X min in such a patient and in eight normal subjects. Euglycemia was maintained by the euglycemic glucose clamp technique, and endogenous insulin secretion was estimated by measuring plasma C-peptide levels. In the normal subjects, plasma C-peptide declined from 1.60 +/- 0.22 (+/- SEM) to 1.16 +/- 0.17 and 0.82 +/- 0.11 ng/ml during the low and high dose insulin infusions, respectively, indicating 27% (P less than 0.01) and 48% (P less than 0.001) decreases in endogenous insulin secretion at moderately elevated and extremely elevated insulin levels, respectively. In the insulinoma patient, plasma C-peptide was 2.6 ng/ml basally, did not change during the low dose insulin infusion, and rose to 3.4 ng/ml during the high dose insulin infusion. We conclude that the feedback regulation of insulin secretion by insulin that occurs in normal subjects is absent in insulinoma patients. This finding could have pathophysiological and possibly diagnostic significance.
[Stress pulsed Doppler echocardiography in the evaluation of diastolic left ventricular function]. (1985)

The effect of sublingual nifedipine on left ventricular (LV) function was assessed by analyzing LV inflow velocity patterns using pulsed Doppler echocardiography (PDE) and radioisotope (RI) angiography in 23 patients with hypertrophic cardiomyopathy (HCM), 13 with hypertensive heart disease (HHD) and 24 with ischemic heart disease (IHD). The results were as follows: LV inflow velocity pattern and M-mode echocardiography after the administration of nifedipine (Study-I). The deceleration time (DT) of the diastolic rapid filling wave was shortened after nifedipine in HCM and HHD, but not in IHD. The acceleration time (AT) of the diastolic rapid filling wave and the A/D ratio (amplitude of an atrial contraction wave/amplitude of a diastolic rapid filling wave) were not significantly changed in all groups. Peak negative Vcf was significantly improved after nifedipine in HCM, but unchanged in the other groups. Fractional shortening and peak Vcf did not change in all groups. LV inflow velocity pattern and RI angiography after nifedipine (Study-II). The mean LV rapid filling rate (V1) based on the LV volume curve by multi-gated blood pool scans increased during nifedipine in HCM and HHD, but other diastolic indices, such as the peak filling rate and 1/3 filling fraction, were unchanged. The DT-V1 relationship during nifedipine shifted toward the right and downward in HCM and HHD. In IHD, however, this relationship shifted toward the left and downward in cases with reduced LV function (V1 less than 1.0 EDC/sec), but toward the right and downward in patients whose LV function was maintained (V1 greater than or equal to 1.0 EDC/sec). Nifedipine therapy was associated with an improved LV filling in nearly all cases of HCM and HHD, and in some cases of IHD. PDE and RI angiography are useful for evaluating LV diastolic function during sublingual nifedipine in various heart diseases.
TMRCC: a congestion control mechanism for tree-based many-to-many reliable multicast protocols (2003)

Congestion control is a key task in reliable multicast along with error control. However, existing tree-based congestion control schemes such as MTCP[11] and TRAMCC[3] are designed for one-to-many reliable multicast and have some drawbacks when they are used for many-to-many reliable multicast. We propose an efficient congestion control mechanism, TMRCC, for tree-based many-to-many reliable multicast protocols. The proposed scheme is based on the congestion windowing mechanism and a rate controller is used in addition. The feedback for error recovery is exploited for congestion control as well to minimize the overhead at the receivers. The ACK timer and the NACK timers are set dynamically reflecting the network condition changes. The rate regulation algorithm in the proposed scheme is designed to help the flows sharing the same link to achieve the fair share quickly. The performance of the proposed scheme is evaluated using ns-2 [2]. The simulation results show that the proposed scheme outperforms TRAMCC in terms of intra-session fairness and shows good level of responsiveness, TCP-friendliness, and scalability.
3D bioprinting using hollow multifunctional fiber impedimetric sensors (2020)

3D bioprinting is an emerging biofabrication process for the production of adherent cell-based products, including engineered tissues and foods. While process innovations are rapidly occurring in the area of process monitoring, which can improve fundamental understanding of process-structure-property relations as well as product quality by closed-loop control techniques, in-line sensing of the bioink composition remains a challenge. Here, we report that hollow multifunctional fibers enable in-line impedimetric sensing of bioink composition and exhibit selectivity for real-time classification of cell type, viability, and state of differentiation during bioprinting. Continuous monitoring of the fiber impedance magnitude and phase angle response from 10<sup>2</sup> to 10<sup>6</sup> Hz during microextrusion 3D bioprinting enabled compositional and quality analysis of alginate bioinks that contained fibroblasts, neurons, or mouse embryonic stem cells (mESCs). Fiber impedimetric responses associated with the bioinks that contained differentiated mESCs were consistent with differentiation marker expression characterized by immunocytochemistry. 3D bioprinting through hollow multifunctional fiber impedimetric sensors enabled classification of stem cells as stable or randomly differentiated populations. This work reports an advance in monitoring 3D bioprinting processes in terms of in-line sensor-based bioink compositional analysis using fiber technology and provides a non-invasive sensing platform for achieving future quality-controlled bioprinted tissues and injectable stem-cell therapies.
Increase in cardiac myosin binding protein-C plasma levels is a sensitive and cardiac-specific biomarker of myocardial infarction. (2013)

Earlier studies have shown that cardiac myosin binding protein-C (cMyBP-C) is easily releasable into the circulation following myocardial infarction (MI) in animal models and patients. However, since its release kinetics has not been clearly demonstrated, no parameters are available to judge its efficacy as a bona fide biomarker of MI in patients with MI. To make this assessment, plasma levels of cMyBP-C and six known biomarkers of MI were determined by sandwich enzyme-linked immunosorbent assay in patients with MI who had before and after Percutaneous Transcoronary Angioplasty (PTCA), as well as healthy controls. Compared to healthy controls (22.3 ± 2.4 ng/mL (n=54)), plasma levels of cMyBP-C were significantly increased in patients with MI (105.1 ± 8.8 ng/mL (n=65), P<0.001). Out of 65 patients, 24 had very high levels of plasma cMyBP-C (116.5 ± 13.3 ng/mL), indicating high probability of MI. Importantly, cMyBP-C levels were significantly decreased in patients (n=40) at 12 hours post-PTCA (41.2 ± 9.3 ng/mL, P<0.001), compared to the patients with MI. Receiver operating characteristic analysis revealed that a plasma cMyBP-C reading of 68.1 ng/mL provided a sensitivity of 66.2% and a specificity of 100%. Also, myoglobin, carbonic anhydrase and creatine kinase-MB levels were significantly increased in MI patients who also had higher cMyBP-C levels. In contrast, levels of cardiac troponin I, glycogen phosphorylase and heart-type fatty acid binding protein were not significantly changed in the samples, indicating the importance of evaluating the differences in release kinetics of these biomarkers in the context of accurate diagnosis. Our findings suggest that circulating cMyBP-C is a sensitive and cardiac-specific biomarker with potential utility for the accurate diagnosis of MI.
Thermal and Electrical Characterization of Silicon Photomultiplier (2008)

Detection of low levels of light is one of the key aspects in medical and space applications. Silicon photomultiplier, a novel type of avalanche photodetector which operates in Geiger mode, shows promising results and offer superior design options. The performance characteristics of the SiPM realized in FBK-irst are studied and presented in this paper. The leakage current, dark rate and internal gain are characterized as a function of temperature. The investigation has been carried out in the framework of the DASiPM Collaboration and the INFN/FBK-irst MEMS project.
ESTIMATION OF CARBOXYL, ALDEHYDE, AND KETONE GROUPS IN CHROMIUM TRIOXIDE OXYSTARCHES (1953)

Under identical conditions, corn starch amylose was more readily oxidized than the amylopectin by chromium trioxide dissolved in acetic acid – acetic anhydride, which was a nonswelling system. These differences in rate nearly vanished for samples dried through solvent exchange when the oxidant was dissolved in a swelling medium, 0.2 M aqueous sulphuric acid. The absolute rate of oxidation, however, was greatly reduced.Carboxyl groups in the oxystarches were satisfactorily estimated either by ion-exchange with calcium acetate or sodium bromide solutions, or by direct titration to about pH 8.5 with aqueous alkali. The samples retained chromium compounds which grossly interfered with the determination of total carbonyl groups by condensation with hydroxylamine hydrochloride; condensation with excess sodium cyanide, and estimation of the ammonia from the hydrolysis of the cyanohydrins, gave better results.Aldehyde groups in an oxystarch containing 0.16 M. of carboxyl and 0.14 M. of carbonyl groups were selectively oxidized with chlorous acid or alkaline hypoiodite, or were selectively condensed with sodium bisulphite solution. All three estimations indicated that about one-third of the carbonyl groups were aldehydes probably occupying the sixth positions in the glucose residues. The cyanohydrin of the oxystarch, when saponified and then hydrolyzed and reduced with boiling hydriodic acid, yielded the lactone of 2-methyl-4-hydroxyhexanoic acid, the recovery of which showed that at least 17% of the carbonyl groups occurred as 2-ketoglucose residues.
Ion‐Selective Electrodes – The Early Years (2011)

Abstract As we near the 50th anniversary of the development of modern ion‐selective electrodes (ISEs), the Matrafüred Conference seemed to be the ideal venue to reminisce about the early years, since Hungary is generally regarded as the birthplace of these new electrodes. Those of us, who worked with ISEs in the 1960s and ′70s, can recall the excitement of discovery existing at the time. In this reminiscence, I shall first provide a few vignettes of the leaders in the early years followed by a description of some of my personal research to demonstrate the variety of novel approaches open to investigators of that era.
Inducible nitric oxide synthase upregulates cyclooxygenase-2 in mouse cholangiocytes promoting cell growth (2004)

Both inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) have been implicated in the biliary tract carcinogenesis. However, it is not known whether these inflammatory mediators are induced by interdependent or parallel pathways. Because iNOS activity has been associated with diverse gene expression, the aim of this study was to determine whether iNOS induces COX-2. To address this objective, immortalized, but nonmalignant, murine cholangiocytes, 603B cells were employed for these studies. Both iNOS and COX-2 protein and mRNA were expressed in these cells. However, iNOS inhibition with either N-[3-(aminomethyl) benzyl]acetamidine or stable transfection with an iNOS antisense construct inhibited COX-2 mRNA and protein expression, an effect that was reversed by NO donors. COX-2 mRNA expression in 603B cells was reduced by pharmacological inhibitors of the p38 MAPK and JNK1/2 pathways. In contrast, neither inhibitors of the soluble guanylyl cyclase inhibitor/protein kinase G nor p42/44 MAPK pathways attenuated COX-2 mRNA expression. Finally, 603B cells grew at a rate threefold greater than 603B-iNOS antisense cells. The low growth rate of 603B-iNOS antisense cells could be restored to near that of the parent cell line with exogenous PGE(2.) In conclusion, iNOS induces COX-2 expression in cholangiocytes, which promotes cell growth. COX-2 induction may contribute to iNOS-associated carcinogenesis.
Improving accuracy in the grading of renal cell carcinoma by combining the quantitative description of chromatin pattern with the quantitative determination of cell kinetic parameters (2000)

The determination of grade and stage in renal cell carcinomas (RCCs) often fails to predict the actual clinical outcome for individual patients. The aim of the present work was to investigate whether it is possible to significantly improve the prognostic accuracy of the grading system by using the combination of two independent computer-assisted microscopy techniques. The first technique relates to the quantitative description of morphonuclear and nuclear DNA content features by means of the image analysis of Feulgen-stained cell nuclei, and the second quantitatively characterizes tumor growth by means of different cell kinetic parameters. These parameters consist of a duplication of a time-related parameter determined by means of the technique of using silver-stained proteins in interphase nucleolar organizer regions (AgNOR), a proliferation index determined by means of MIB-1 immunohistochemistry, and an apoptotic index determined by means of the terminal dUTP nick end labeling technique. The prognostic value of these quantitative features was investigated in a series of 60 RCCs. The quantitative analysis of Feulgen-stained nuclei made it possible to identify subgroups of patients with significantly different prognoses in both grade II and grade III RCCs. We labeled the RCCs associated with the most favorable prognoses as grade II- and III- and those with the least favorable ones as grade II+ and III+. The two most important kinetic variables to identify patients with different clinical outcomes were the MIB-1 index and the mean AgNOR area in the MIB-1-positive cells. Three significantly different survival curves were obtained for the 53 grade II and III RCC patients. Our results show that conventional RCC grading can be significantly improved by the quantitative analysis of Feulgen-stained nuclei, by cell kinetic parameter determination, and, more importantly, by combining the proliferation index with the mean AgNOR area parameter.
Flavor of Palm Oil (1980)

It is known that palm oil has a peculiar flavor. However, little information has been obtained on this flavor. To know the flavor compounds characteristic for palm oil, palm oil was fractionated into several fractions by vacuum distillation and saponification, and the organoleptic test of each fraction was carried out. The results indicate that carbonyl-free fractions separated from volatile compounds have distinct palm oil-like flavor. In an other experiment, β-carotene was heated at 200°C for 20min and fractionated into eight fractions by TLC and it was recognized that some fractions showed palm oil-like taste. It seemed to be important for reducing palm oil flavor to remove thermal decomposition products of β-carotene as much as possible.
Blind sparse inpainting reveals cytoskeletal filaments with sub-Nyquist localization (2017)

Single-molecule localization microscopy (SMLM), such as stochastic optical reconstruction microscopy and (fluorescence) photoactivated localization microscopy, has enabled superresolution microscopy beyond the diffraction limit. However, the temporal resolution of SMLM is limited by the time needed to acquire sufficient sparse single-molecule activation events to successfully construct a superresolution image. Here, a novel fast SMLM technique is developed to achieve superresolution imaging within a much shortened duration. This technique does not require a faster switching rate or a higher activation density, which may cause signal degradation or photodamage/bleaching, but relies on computational algorithms to reconstruct a high-density superresolution image from a low-density one using the concept of blind image inpainting. Our results demonstrate that the technique reduces the acquisition time by up to two orders of magnitude compared to the conventional method while achieving the same high resolution. We anticipate our technique to enable future real-time live cell imaging with even higher resolution.
Linear and bilinear magnetoelectric effects in magnetically biased magnetite (1976)

In a synthetic magnetite (Fe3O4) crystal at 4.2°K the macroscopic anisotropy coefficients depend1 on an external electric field E↘. We now show that this dependence gives rise to linear as well as to two kinds of bilinear magnetoelectric (ME) effects provided the crystal is biased by means of an external static magnetic field H↘0. Upon expanding the previously proposed1 free energy density to F̃ to third order in the perturbing fields E↘ and h↘=H↘−H↘0, we obtain terms (among others) of the form −PoiEi, −αijEihj, −1/2βijkEihjhk and −1/2γijkEiEjhk, where, i,j,k denote a,b or c. By requiring the magnetization M↘ to be in equilibrium in each order of the perturbation, we calculate, without adjustable parameters, the dependence on H↘0 of P0i (obtained previously1) and of the ME susceptibilities αij, βijk and γijk. We measure the dependence on H↘0 of these three susceptibilities by using 1 kHz fields for E↘ and h↘ and observing the 2 kHz as well as the 1 kHz parts of both P↘ (=−∂F̃/∂E↘) and M↘ (=−∂F̃/∂H↘). For h↘∥c↘ (= easy axis) and H↘0 oriented arbitrarily in the bc plane, the measured H↘0‐dependence of αac agrees very well with the single‐domain‐rotation version of the theory whenever H0 is sufficiently large and H↘0 is not parallel to b↘. For h↘∥H↘0∥b↘ (=intermediate axis), the measured H0b‐dependences of αab and βabb all agree reasonably well with the two‐domain‐rotation version of the theory at all values of H0b. Our measured ‖βabb‖ at H0=0 is also calculable and is the largest ‖βijk‖ ever observed in any material.