gebhart/patrepeval huggingface dataset:
Novel device prototyping for endoscopic cell sheet transplantation using a three-dimensional printed simulator (2020)

We developed a novel prototype of endoscopic cell sheet delivery device for minimally-invasive cardiac regenerative therapy utilizing a 3D printed simulator. The commercialization of the prototype may provide a safe minimally-invasive method to deliver potential cardiac regenerative therapy in the future.
Promotion of Micrometastasis Proliferation in a Rat Rhabdomyosarcoma Model by Epidermal Growth Factor (1989)

Using a rat rhabdomyosarcoma 9-4/0, we investigated the role of epidermal growth factor (EGF) in tumor dissemination. In vitro, we detected high-affinity EGF receptors on tumor cells and stimulation of their proliferation by EGF. When injected iv, EGF-pretreated cells demonstrated an increased capacity to form lung colonies and to invade lymphatic tissue. In vivo, EGF treatment led to increased metastatic spread of subcutaneous tumors. When primary tumors were ablated, and the treatment was given from the time of graft until ablation (seeding step), no effect on metastatic spread was noticed. When treatment was given from the time of ablation until death (growth step), EGF increased the number of lung metastases and of invaded lymph node sites.
La práctica de la evaluación del riesgo de violencia en España (2015)

La valoración del riesgo de violencia es un requisito fundamental en la toma de decisiones profesionales que implican prevenir, intervenir o informar sobre la conducta de las personas. El uso de herramientas estructuradas mejora la precisión de las evaluaciones basadas en el juicio clínico en contextos psiquiátricos, penitenciarios y jurídicos. Este estudio presenta resultados de la primera encuesta sobre el uso de herramientas de evaluación del riesgo de violencia y sobre su utilidad percibida en España. Las escalas de psicopatía (PCL-R y PCL:SV) y el HCR-20 encabezaron la lista de las herramientas más usadas tanto por elección personal como por requisito institucional. Se ofrecen datos novedosos sobre las prácticas profesionales de evaluación del riesgo de violencia que pueden orientar a los profesionales que desempeñan su tarea en contextos sanitarios, correccionales y forenses, donde los instrumentos estructurados son frecuentemente usados para asistirlos en la toma de&lt;br /&gt;decisiones.
Data from Forced Activation of β-Catenin Signaling Supports the Transformation of <i>hTERT</i>-Immortalized Human Fetal Hepatocytes (2023)

&lt;div&gt;Abstract&lt;p&gt;Hepatocarcinogenesis is a multistep process driving the progressive transformation of normal liver cells into highly malignant derivatives. Unlimited proliferation and telomere maintenance have been recognized as prerequisites for the development of liver cancer. Moreover, recent studies identified illegitimate β-catenin signaling as relevant hit in a considerable subset of patients. To further investigate the currently not well-understood malignant evolution driven by telomerase and β-catenin, we monitored cytogenetic and phenotypic alterations in untransformed telomerase-immortalized human fetal hepatocytes following forced activation of β-catenin signaling. As expected, constitutive activation of β-catenin signaling significantly enhanced proliferation with decreasing serum dependence. Previously intact contact inhibition was almost completely eliminated. Interestingly, after several passages in cell culture, immortalized clones with dominant-positive β-catenin signaling acquired additional chromosomal aberrations, in particular translocations, anchorage-independent growth capabilities, and formed tumors in athymic nude mice. In further support for the driving role of β-catenin during hepatocarcinogenesis, improved colony growth in soft agar and accelerated tumor formation was also confirmed in Huh7 cells following stable expression of the constitutively active S33Y β-catenin mutant. Telomerase inhibition showed that short-term expansion of transformed clones was not telomerase dependent. Finally, cancer pathway profiling in derived tumors revealed upregulation of characteristic genes associated with invasion and angiogenesis. In conclusion, illegitimate activation of β-catenin signaling enhances the transformation from immortalization to malignant growth in human fetal hepatocytes. Our data functionally confirm a permissive role for β-catenin signaling in the initial phase of hepatocarcinogenesis. &lt;i&gt;Mol Cancer Res; 9(9); 1222–31. ©2011 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Two-stage reverse osmosis: optimal element configuration and energy savings (2017)

RO desalination can help to ensure secure water resources now and in the future, but the process remains energy intensive. Improving RO’s energy efficiency is thus an important step towards achieving a sustainable water supply. While innovations in membrane and pump technology are not likely to substantially decrease the energy consumption of the RO process, improved system designs have real potential to bring RO closer to its thermodynamic performance limit. Two-stage systems can substantially lower RO energy consumption. In a fixed size two-stage reverse osmosis (RO) system with eight membrane elements, the elements can be shared between the two stages in seven distinct element configurations. In this work, we investigate the optimal element configuration (system design) of a two-stage RO system. We isolate the energetic benefits of staging by comparing the energy consumption of a two-stage RO system to that of a single-stage RO system with the same system size and freshwater productivity. The optimal element configuration will place at least half of the elements in the first stage; the exact configuration depends on feed salinity, recovery ratio, and membrane permeability. Previous studies on the energetic benefits of two-stage RO have not accounted for both the system size and the effects of concentration polarization. We evaluate systems with an average system flux comparable to today's systems and account for frictional losses and the effects of concentration polarization. This results in a more realistic evaluation of the energetic benefits of two-stage RO. More energy can be saved by adding a stage when the thermodynamic least work of separation is larger. Therefore, energy savings from adding a second stage grow as recovery ratio increases. Significant energy can be saved with high salinity feeds at relatively low recovery ratios. We find that significant energy can be saved with the simplest two-stage RO design, at a system flux similar to today's RO plants and accounting for the effects of concentration polarization. We perform a brief economic analysis to compare the relative capital expenses to the reduction in specific energy consumption (SEC) associated with a two-stage RO plant. We find that two-stage RO is probably not viable for seawater desalination at today’s typical recovery ratios. If recovery ratios can be pushed up to 60%, two-stage RO could become viable with favorable financing terms and high cost of electricity.
Reversible aggregation of gold nanoparticles driven by inclusion complexation (2007)

In this work, gold nanoparticles with an appreciably narrow size distribution are prepared by reduction of HAuCl4 in the presence of mono-6-SH-β-cyclodextrins (CDs). The β-CD modified gold nanoparticles (β-CD-AuNPs) tend to aggregate together upon addition of diazo, water soluble ditopic guest molecules with a double-azobenzene structure, due to the host–guest inclusion complexation between β-CD and diazo. The aggregation leads to significant shifts in the absorption spectrum with concomitant visible color changes from pinkish-red to purple. Furthermore, the average number of gold particles in each gold aggregate is controllable as it depends on the molar ratio of diazo to β-CD. Further disassociation of the gold aggregation can be realized by addition of excess competitive host α-CD which favors forming inclusion complexes with the guest more than β-CD does. The aggregation–dissociation of β-CD-AuNPs is confirmed to be reversible. This simple, yet highly efficient way to control the self-assembly of gold nanoparticles could be helpful in repeated usage of the gold nanoparticles applied to diverse technologies.
Osteoarthritis in rhesus macaque knee joint: quantitative magnetic resonance imaging tissue characterization of articular cartilage. (1995)

Topological quantitative MRI relaxation time assessment demonstrates increasing cartilage matrix quality variation with OA progression.
Evaluation of Antiviral Efficacy Against Human Respiratory Syncytial Virus Using Cotton Rat and Mouse Models (2013)

Infection with human respiratory syncytial virus (hRSV) causes a wide spectrum of respiratory disease in infants, young children, and elderly persons. No vaccine is available today and hRSV treatment options are limited. As a consequence, the treatment of hRSV infection remains largely supportive and new therapeutic options are needed to treat severe lower respiratory tract hRSV disease. Several animal models have been developed to study hRSV disease and evaluate novel therapies or preventive measures such as vaccines. However, each of these models reproduces different aspects of hRSV disease, and therefore, an appropriate model should be selected on the basis of the scientific question under investigation. In this chapter, we describe how cotton rats and Balb/c mice are used in our laboratory to test the in vivo efficacy of small-molecule inhibitors against hRSV.
Panel of Engineered Ubiquitin Variants Targeting the Family of Human Ubiquitin Interacting Motifs (2022)

Ubiquitin (Ub)-binding domains embedded in intracellular proteins act as readers of the complex Ub code and contribute to regulation of numerous eukaryotic processes. Ub-interacting motifs (UIMs) are short α-helical modular recognition elements whose role in controlling proteostasis and signal transduction has been poorly investigated. Moreover, impaired or aberrant activity of UIM-containing proteins has been implicated in numerous diseases, but targeting modular recognition elements in proteins remains a major challenge. To overcome this limitation, we developed Ub variants (UbVs) that bind to 42 UIMs in the human proteome with high affinity and specificity. Structural analysis of a UbV:UIM complex revealed the molecular determinants of enhanced affinity and specificity. Furthermore, we showed that a UbV targeting a UIM in the cancer-associated Ub-specific protease 28 potently inhibited catalytic activity. Our work demonstrates the versatility of UbVs to target short α-helical Ub receptors with high affinity and specificity. Moreover, the UbVs provide a toolkit to investigate the role of UIMs in regulating and transducing Ub signals and establish a general strategy for the systematic development of probes for Ub-binding domains.
Development of New Anchor Impellers for Non-Newtonian Fluid in a Rotating Vessel (2018)

塑性流体や粘弾性流体などの非ニュートン流体の混合に適した新しいアンカー翼を開発した．この新しい混合システムは，非定常な回転槽と新しいアンカー翼を組み合わせることにより，非ニュートン流体を容易に混合することができた．また，粘弾性流体に対し，偏心板型シャフトを使って混合すると特異的なフローパターンが発生することを見出し，新型アンカー翼のシャフト形状に反映した．
Transient Stability Analysis of AC-DC Hybrid Power Systems Using Direct Method (2018)

Large-scale AC-DC hybrid power systems have become a basic pattern for power transmission in China. Currently, time-domain simulation is widely used for transient stability analysis, which has the disadvantages of high computational burdens and lack of system stability margin information. An alternative method for transient stability analysis is the direct method, which assesses transient stability from the energy point of view. Direct method requires much less computation efforts. In addition, the stability margin of the system can be obtained. Due to the challenges in system modelling, energy function construction, and determination of critical energy value, it is challenging to apply direct method for transient stability analysis of AC-DC hybrid power systems. This paper is a step in exploring the application of direct method in AC-DC hybrid power systems.
Data from Wnt5a Drives an Invasive Phenotype in Human Glioblastoma Stem-like Cells (2023)

&lt;div&gt;Abstract&lt;p&gt;Brain invasion by glioblastoma determines prognosis, recurrence, and lethality in patients, but no master factor coordinating the invasive properties of glioblastoma has been identified. Here we report evidence favoring such a role for the noncanonical WNT family member Wnt5a. We found the most invasive gliomas to be characterized by Wnt5a overexpression, which correlated with poor prognosis and also discriminated infiltrating mesenchymal glioblastoma from poorly motile proneural and classical glioblastoma. Indeed, Wnt5a overexpression associated with tumor-promoting stem-like characteristics (TPC) in defining the character of highly infiltrating mesenchymal glioblastoma cells (Wnt5a&lt;sup&gt;High&lt;/sup&gt;). Inhibiting Wnt5a in mesenchymal glioblastoma TPC suppressed their infiltrating capability. Conversely, enforcing high levels of Wnt5a activated an infiltrative, mesenchymal-like program in classical glioblastoma TPC and Wnt5a&lt;sup&gt;Low&lt;/sup&gt; mesenchymal TPC. In intracranial mouse xenograft models of glioblastoma, inhibiting Wnt5a activity blocked brain invasion and increased host survival. Overall, our results highlight Wnt5a as a master regulator of brain invasion, specifically TPC, and they provide a therapeutic rationale to target it in patients with glioblastoma. &lt;i&gt;Cancer Res; 77(4); 996–1007. ©2016 AACR&lt;/i&gt;.&lt;/p&gt;&lt;/div&gt;
Problem definition in a participatory design process. (1995)

Although there exist substantial literatures on individual design processes and upon problem definition, there is little attention to how participatory design groups define the problems on which they work. This lack occurs because of the difficulty in learning about what goes on inside design groups. However, participatory and collaborative design is a widespread and growing mode of design practice that demands a better understanding. This dissertation consists of an in depth study of one participatory design process that occurred in a federal laboratory setting. It was a multi-disciplinary activity that initially involved representatives from five research and development departments. These representatives collaborated over seven months to create a project proposal that would incorporate technology and design contributions from each of their specialities. The final proposal comprised a design problem definition including specific design components, schedule, budget, and system integration requirements. The central thesis states that collaborative or participatory design is especially appropriate for defining a design problem, as opposed to systematic design methods that are more appropriate for seeking a solution to that design problem. Several propositions devolve from this thesis, notably the idea of alternative paths toward a common goal. The research approach was research-in-action. The author served as a facilitator of the participatory design process, recording their activities and the outcomes of their efforts. The facilitators developed and employed a number of participatory design methods to address specific issues and to encourage the participants to build a consensus upon a proposal. Most of these methods failed to produce the intended results but at least one succeeded for each issue, and eventually the participants took the initiative to forge the essential consensus. This consensus proved critical to the proposal's success. The analysis of the results relies upon direct observation and upon an extensive literature review of precedents in design participation. The attendance data reveal that most of the facilitator and participant interaction occurred outside the formal meetings. A meta-analysis of the observations shows they are largely consistent with precedent, plus a few important new insights. The findings indicate that the design problem definition moved through a sequence of states known in the literature: from "condition of irresolution," to, "wicked problem," to "ill-defined" problem, to a final "well-structured" problem state. The participatory design group's consensus upon the project content and internal relationships proved a crucial step in forging the final design problem definition. The conclusion presents a new model of design participation, in which the design group's consensus upon a problem definition acts as a gateway between problem-defining and solution-seeking.
Seabed Surveys to Document Groundings from the 2000 Grand Bank Iceberg Season (2003)

The 2000 iceberg season provided the first recent opportunity to investigate and develop case histories of reported iceberg groundings on Grand Bank. In 2001, the locations for eight of nine icebergs reported as grounded were investigated with sidescan sonar, Huntec sub-bottom profiler, a towed underwater camera and a light Work Class ROV. In addition to the new scours, re- surveys in 2001 extended the time series for three groundings from the 1980s. In 2002, swath bathymetric surveys were conducted to provide detailed scour depth and shape information. The 2001 and 2002 seabed surveys documented six seabed scour features attributable to year 2000 groundings. Three icebergs produced both a furrow and a terminal pit, and three produced isolated circular pits with little evidence of significant furrows leading into or out of the pit. One of the pitting icebergs produced one pit; the other two produced two pits each. Methods applied to measure scour depths from multibeam data are described for a pit and for a furrow.
On Digital Power Transmission Systems (2019)

In this paper, the integration of digital transmission system and power transmission system using switching power converter is presented. The proposed concept can be referred as a Digital Power Transmission system. The aim is to provide electric power for residential, commercial and industrial loads as well as to deliver data, audio or video information to customers. This paper focused on Digital DC Power Transmission system based on buck converter topology. The proposed digital power transmission system has specification of 400 V dc input voltage, 2 Mbps data rate with unipolar NRZ (Non-Return to Zero) line coding and three point of loads in the range of 1.1 - 1.9 kW. The voltage and current stresses of semiconductor devices will be analyzed in this paper. In addition, the behavior of current and voltage waveforms will be simulated and discussed. The analysis of the converter in CCM (Continuous Conduction Mode) operation based on digital input signal is also provided. The method to extract and separate DC output power from digital output signal is also discussed. Simulation results confirm that the digital output signal with data rate of 2 Mbps is the same as the digital input signal, and the output power can be extracted to the loads without introducing harmful functions.
Mobile robot localization using circular correlations of panoramic images (2002)

In order to determine both location and orientation of a mobile robot, we use omni-directional view images from a camera mounted on top of the robot. Images are sampled circularly on rings at various radii. Correlation values between rings of different images are regarded as the similarity of the image, therefore the similarity of locations. Using real images taken at different times in navigation experiments, we computed correlation values normalized by brightness, and confirmed the robot can be localized in 50 cm to 100 cm range with 3-10 degree orientation accuracy. The idea of using aggregated sum of circular samples for representing landmarks at multiple levels is also presented.
Catalytic Action of Azolium Salts. IX. Synthesis of 6-Aroyl-9H-purines and Their Analogues by Nucleophilic Aroylation Catalyzed by Imidazolium or Benzimidazolium Salt. (1998)

In the presence of 1, 3-dimethylimidazolium iodide (1), 6-chloro-9-phenyl-9H-purine (7) and 4-chloro-5, 6-dimethylpyrrolo[2, 3-d]pyrimidines 40-42 undrwent uncleophilic aroylation with arenecarbaldehydes (5) to give the corresponding fused aroylpyrimidines 8 and 43-45. 1, 3-Dimethylbenzimidazolium iodide (2) was an effective catalyst for the similar synthesis of 7-aroyl-3-phenyl-3H-1, 2, 3-triazolo[4, 5-d]pyrimidines 16-21. In the synthesis of 4-aroyl-1H-pyrazolo[3, 4-d]pyrimidines 26-32, both azolium salts 1 and 2 were effective as catalysts. Moreover, 4-aroyl-7H-pyrrolo[2, 3-d]pyrimidines 43-45 were obtained in good yields via the 4-tosyl derivatives, in the presence of catalytic amounts of sodium p-toluenesulfinate (46) and the imidazolium salt 1. This catalytic aroylation was found to be a facile and useful method for the synthesis of 6-aroyl-9H-purines and their analogues.
Headache (2012)

The ICHD-II divides headaches into primary and secondary disorders. With ageing, not only is there a change in prevalence of the primary headache disorder but also a shift to new or organic causes of headache. Evaluation of the elderly patient with headache must rule out serious secondary causes of headache, such as tumour, subdural haematoma, stroke, transient ischaemic attack and temporal arteritis. Medications that are more commonly used in the elderly may exacerbate or trigger migraine. In the elderly, migraine aura without headache (acephalic migraine) is also called late-life migraine accompaniments. Hypnic headache is a rare, strictly nocturnal headache that occurs in older persons. Giant cell arteritis occurs in 3–9/100 000 patients over the age of 50 years, with women affected three times more often than men. The association between Parkinson's disease and headache is controversial. Trigeminal neuralgia is the most common neuralgic syndrome in the elderly, with a peak incidence in the sixth and seventh decades.
Automatic control of constant highly reflective loads in active load-pull systems for the characterization of compression effects and IM3 in SSPAs (1993)

Optimization of trade-offs between power added efficiency and third order intermodulation (IM3) in transistors is the most critical point encountered in the design of communication power amplifiers. Power transistors are generally highly mismatched components. The characterization of such devices by load-pull techniques is a very difficult task. We have recently proposed a solution to this problem. It consists in using appropriate mismatched power sources to drive the device under test (DUT) during the measurement process [1]. Prediction of IM3 can be achieved by computing the measured single tone complex gain of the DUT for any load impedance of interest. Nevertheless, in active load-pull systems the load impedance doesn't remain constant when the power level driving the input of the DUT varies. Therefore, it is necessary to compute measurement data files in order to extract the complex envelope gain function of the DUT. The modified active load-pull set-up proposed in this paper allows the control of any constant load impedance of interest. Any desired load impedance is automatically kept constant independently of the input driving signal of the DUT. In consequence, complex gain can be directly extracted from measurements and IM3 quickly predicted.
Human motion energy harvesting using a piezoelectric MFC patch (2015)

The improvements in efficiency of electronic components and miniaturization is quickly pushing wearable devices. Kinetic human energy harvesting is a way to power these components reducing the need of batteries replacement since walking or running is how humans already expend much of their daily energy. This work explores the case of kinetic energy from bending of a piezoelectric patch. For assessing the quality of the system, a testing setup has been designed and controlled by means of knee joint recordings obtained from a large motion dataset. The promising result of the chosen patch is an output power of 2.6μW associated to a run activity.