gebhart/patrepeval huggingface dataset:
The Absence of Cecal Colonization of Chicks by a Mutant of Campylobacter jejuni Not Expressing Bacterial Fibronectin-Binding Protein (1999)

Campylobacter jejuni is a common cause of human gastrointestinal illness throughout the world. Infections with C. jejuni and Campylobacter coli are frequently acquired by eating undercooked chicken. The ability of C. jejuni to become established in the gastrointestinal tract of chickens is believed to involve binding of the bacterium to the gastrointestinal surface. A 37-kD outer membrane protein, termed CadF, has been described that facilitates the binding of Campylobacter to fibronectin. This study was conducted to determine whether the CadF protein is required for C. jejuni to colonize the cecum of newly hatched chicks. Day-of-hatch chicks were orally challenged with C. jejuni F38011, a human clinical isolate, or challenged with a mutant in which the cadF gene was disrupted via homologous recombination with a suicide vector. This method of mutagenesis targets a predetermined DNA sequence and does not produce random mutations in unrelated genes. The parental C. jejuni F38011 readily colonized the cecum of newly hatched chicks. In contrast, the cadF mutant was not recovered from any of 60 chicks challenged, indicating that disruption of the cadF gene renders C. jejuni incapable of colonizing the cecum. CadF protein appears to be required for the colonization of newly hatched leghorn chickens.
Potential of Sugarcane in Modern Energy Development in Southern Africa (2016)

For more than half of the Southern African population, human development is limited by a lack of access to electricity and modern energy for cooking. Modern bioenergy merits consideration as one means to address this situation in areas where sufficient arable land is available. While numerous studies have concluded that Africa has significant biomass potential, they do not indicate by how much it can effectively reduce the use of traditional biomass and provide more accessible energy, especially at a country level. Here, we evaluate the potential of sugarcane to replace traditional biomass and fossil fuel, and enlarge the access to electricity in Southern Africa. By using its current molasses for ethanol production, Swaziland could increase electricity generation by 40% using bagasse, and replace 60% of cooking fuel or 30% of liquid fossil fuel. Sugarcane expansion over 1% of the pasture land in Angola, Mozambique, and Zambia could replace greater than 70% of cooking fuel. Bioelectricity generation from modest sugarcane expansion could be increased by 10% in Malawi, Mozambique and Zambia, and by 20% in Angola. Our results support the potential of sugarcane as a modern energy alternative for Southern Africa.
Global States of a Distributed System (1982)

A global state of a distributed transaction system is consistent if no transactions are in progress. A global checkpoint is a transaction which must view a globally consistent system state for correct operation. We present an algorithm for adding global checkpoint transactions to an arbitrary distributed transaction system. The algorithm is nonintrusive in the sense that checkpoint transactions do not interfere with ordinary transactions in progress; however, the checkpoint transactions still produce meaningful results.
Dual-band CPW-fed G-antenna using an EBG structure (2010)

International audience
Adaptive Target Detection With Application to Through-the-Wall Radar Imaging (2010)

An adaptive detection scheme is proposed for radar imaging. The proposed detector is a postprocessing scheme derived for one-, two-, and three-dimensional data, and applied to through-the-wall imaging using synthetic aperture radar. The target image statistics depend on the target three-dimensional orientation and position. The statistics can also vary with the standoff distance of the imaging system because of the change in the corresponding scene image resolution. We propose an iterative target detection scheme for the cases in which no or partial a priori knowledge of the target image statistics is available. Properties of the proposed scheme, such as conditions of convergence and optimal configurations are introduced. The detector performance is examined under synthetic and real data. The latter is obtained using a synthetic aperture through-the-wall radar indoor imaging scanner implementing wideband delay and sum beamforming.
Poly(ethylene glycol-co-allyl glycidyl ether)s: A PEG-Based Modular Synthetic Platform for Multiple Bioconjugation (2011)

A series of random copolymers comprising ethylene oxide (EO) and 0-100% allyl glycidyl ether (AGE) has been prepared by anionic ring-opening polymerization with molecular weights between 5000 and 13,600 g/mol and polydispersity indices in the range of 1.04-1.19. As key for the homogeneity of the PEG conjugates, real-time ¹H NMR polymerization kinetics, ¹³C NMR analysis of triad sequence distribution, and analysis of the thermal behavior by differential scanning calorimetry (DSC) revealed a distinctive random copolymer structure. Via thiol-ene coupling (TEC), showing mainly "click" characteristics and nearly quantitative yields, PEG derivatives with multiple amino, carboxy, or hydroxy functionalities have been prepared, providing suitable reactivities for further attachment. Without further modification, P(EO-co-AGE)s were conjugated with cysteine or the tripeptide glutathione (GSH) via TEC, resulting in well-defined hybrid materials with multiple peptide units conjugated to the PEG backbone. The results demonstrate superior loading capacity of the copolymers in comparison to the PEG homopolymer.
Identification of Post-translational Modifications on Odorant-Binding Protein Isoforms from Pig Olfactory Secretome by High-Resolution Mass Spectrometry: O-β-N-acetylglucosaminylation and Phosphorylation (2017)

Odorant-Binding Proteins (OBP) are major players of perireceptor events in olfaction. Despite their importance, a molecular mechanism explaining their specificity for odors and pheromones has yet to be proposed. A new approach is provided by the analysis of the pig olfactory secretome that is mainly composed of OBP isoforms, generated from 3 gene products by two types of post-translational modifications (PTM): i) phosphorylation and ii) O--N-acetylglucosaminylation (O-GlcNAcylation), which are unusual for secreted proteins. Although both types of PTM can be demonstrated on OBP isoforms by specific antibodies, they have to be identified by mass spectrometry (MS), as localizing PTM sites and identifying PTM patterns can help predict binding affinities. In this paper, we report the identification of phosphorylation and O-GlcNAcylation sites on peptides coming from trypsin digestion of only OBP (sensu stricto) by nanoLC-nanoESI-HCD-MS/MS-Orbitrap. These PTM were not present on VEG and SAL. PEAKS software analysis of raw MS data allowed selecting spectra that were analyzed manually to identify PTMs. Four peptides corresponding to two different portions of OBP sequence were modified either by a phosphate group or by a hexNAc moiety. Due to the high energy used in HCD, the data did not allow precise localization of the modified sites. The new findings contribute to a better understanding of the mechanisms by which OBP isoforms could extend the binding repertoire of the secreted OBPs. Data are available via ProteomeXchange with identifier PXD007955.
Modulation of vastus medialis motoneuronal excitability by sciatic nerve afferents (1998)

We activated the sciatic nerve afferents by either the discharge of a magnetic coil or a needle inserted near the nerve. Both types of stimulation induced facilitation of the vastus medialis (VM) H reflex, and of the VM response to transcranial magnetic stimulation, at the joint time of arrival of conditioning and test volleys, while a subsequent inhibition was induced only in the H reflex. We conclude that sciatic nerve afferents induce reciprocal inhibition of VM motoneurons presynaptically on the Ia afferent terminals. © 1998 John Wiley & Sons, Inc. Muscle Nerve 21:936–939, 1998.
Supplementary Figure 3 from An Integrated Genomic Screen Identifies LDHB as an Essential Gene for Triple-Negative Breast Cancer (2023)

&lt;p&gt;PDF file - 1026K, LDHA levels do not fluctuate after LDHB knockdown&lt;/p&gt;
Influence of the Linking Number of Template DNA on .SIGMA.32-Dependent Transcription Initiation in Vitro of the Escherichia coli groE Gene. (1996)

Plasmids carrying the groE promoter with a superhelical density of 0 to -0.091 were used for in vitro transcription with RNA polymerase holoenzyme containing sigma 32. The promoter activity of the groE gene in vitro on the plasmid DNA was decreased to 100-fold when the superhelical density of the template DNA decreased from -0.059 to 0. As the superhelical density of DNA inside the cells was estimated to be approximately -0.025, it seems likely that the increase in the linking number of template DNA caused by stress which induces heat shock response may not stimulate the groE promoter activity in vivo on the chromosomal DNA. At higher concentrations of salt, the dependence of the groE promoter activity on negative supercoiling was even more apparent. Since melting of the DNA duplex is inhibited by high salt concentrations, DNA supercoiling may facilitate melting of the groE promoter by an RNA polymerase holoenzyme containing sigma 32.
A 47-Year-Old Man With Chronic Depression (1996)

<h3>Dr Delbanco:</h3> Mr N is a 47-year-old man who has struggled with depression for approximately 15 years. Divorced from his first wife and now separated from his second, he is the father of two teenaged children. He works for a local human service institution and receives private managed care health insurance through his employer. At 32 years of age, Mr N sought marriage counseling with his first wife. After his marriage ended, he continued with individual counseling; a diagnosis of depression was established, and he began taking an antidepressant he believes to have been imipramine. In 1980, he moved to Massachusetts and was denied coverage for individual psychotherapy by his new health plan. He was offered group counseling but after three meetings found it an inappropriate setting for his problems. Between 1983 and 1984, Mr N felt markedly worse and quit his job. During this time his divorce became final.
Tumor-selective, adenoviral-mediated GFP genetic labeling of human cancer in the live mouse reports future recurrence after resection (2011)

We have previously developed a telomerase-specific replicating adenovirus expressing GFP (OBP-401), which can selectively label tumors in vivo with GFP. Intraperitoneal (i.p.) injection of OBP-401 specifically labeled peritoneal tumors with GFP, enabling fluorescence visualization of the disseminated disease and real-time fluorescence surgical navigation. However, the technical problems with removing all cancer cells still remain, even with fluorescence-guided surgery. In this study, we report imaging of tumor recurrence after fluorescence-guided surgery of tumors labeled in vivo with the telomerase-dependent, GFP-containing adenovirus OBP-401.. Recurrent tumor nodules brightly expressed GFP, indicating that initial OBP-401-GFP labeling of peritoneal disease was genetically stable, such that proliferating residual cancer cells still express GFP. In situ tumor labeling with a genetic reporter has important advantages over antibody and other non-genetic labeling of tumors, since residual disease remains labeled during recurrence and can be further resected under fluorescence guidance.
CD44 protein levels and its biological activity are regulated in Balb/c 3T3 fibroblasts by serum factors and by transformation with the ras but not with the sis Oncogene (1996)

CD44s (standard isoform) levels and hyaluronan-binding activity were investigated in Balb/c 3T3 cells and their derivatives transformed with ras or sis oncogenes as a function of serum concentration in the medium. 3T3 cells contained low levels of CD44 and did not bind hyaluronan when grown in medium containing 0.5 or 10% serum. In 5% serum, however, the cells had much higher levels of CD44 and were able to bind hyaluronan. CD44 levels also increased in 3T3 cells restimulated with either 5 or 10% serum after prior maintenance in low serum. In cells restimulated with 5% serum, high levels of CD44 were sustained for at least 72 hr. In cells restimulated with 10% serum, however, the increase in CD44 levels reverted by 48 hr. Transformation of 3T3 cells with ras (but not with sis) oncogene rendered CD44 levels insensitive to serum modulation: ras-transformed cells contained high levels of CD44 and bound hyaluronan at all serum concentrations and at all time points tested. Sis-transformed cells behaved like 3T3 cells in these modulatory changes. Platelet-derived growth factor (PDGF), when supplementing 0.5% serum, mimicked the effects of serum on the levels and hyaluronan-binding capacity of CD44 in 3T3 cells and the CD44-upregulating activity of serum was neutralized by incubation with anti-PDGF antibodies. These data demonstrate that serum factors, specifically PDGF, mediate regulation of CD44 levels in Balb/c 3T3 cells and that transformation of 3T3 cells by ras renders CD44 expression insensitive to the modulating effects of serum in vitro. These results correlate with the metastatic capacity of these cells in vivo. © 1996 Wiley-Liss, Inc.
A Theoretical Model for Investigating the Structural Dynamics of a Rolling Tyre (2022)

Abstract The noise generated by the rolling tyre contributes significantly to the car's interior noise. This is caused by the tyre-road contact, and at low frequencies (0–500 Hz) is mostly transmitted inside the cockpit through the structure-borne transmission path. In support of the studies in this research field, an interpretative model of the tyre-wheel system accounting for the effects induced by the angular speed represents a useful tool. To this aim, we implemented an analytical model based on a flexible ring on an elastic foundation to analyze the dynamics of the tyre-wheel system, in both static and rotating configurations. We fine-tuned the parameters of the tyre based on data coming from experimental modal analysis of the static tyre. Particular attention has been paid to the system's free and forced responses, commonly analyzed with the so-called cleat test. The results are discussed interpreting the behavior in different reference systems.
특집(特輯) : 2007년 국민편의를 위한 법조 개혁 방안 ; 공판중심주의의 구현 방안 (2007)

우리 형사소송법 제정 당시부터 근본이념으로 되어 있는 공판중심주의는 형사사법의 중심이 공판에 있다는 것을 말한다. 종래 우리 형사소송절차 전반에 나타난 축약과 간이화, 조서재판의 실무관행을 극복하고, 법정에서 이루어진 생생한 증거조사결과를 토대로 심증을 형성하자는 것이다. 공판중심주의는 적법절차와 피고인의 인권보장, 법정심리의 충실화와 절차적 정당성 제고, 집중심리, 소송관계인의 절차적 만족감 제고, 합리적이고 적정한 양형을 기본방향으로 하고, 선택과 집중의 원리, 알기 쉽고 신속·정확한 심리를 그 구현원리로 하고 있다. 먼저, 공판준비에서는 피고인과 변호인의 수사기록 열람·등사(즉, 증거개시)가 중요한데, 이는 무기대등의 원칙과 공판절차의 효율, 신속·공정한 재판을 위해 필수적인 것이며, 법의 일반원칙으로까지 승화된 당연한 강제규범이라고까지 할 수 있다. 모두절차에서는 검사의 기소요지 진술과 피고인·변호인의 모두진술을 충실하게 운영해 나갈 필요가 있다. 피고인신문절차의 기능 중 증거조사의 기능을 지나치게 강조하게 되면 피고인을 절차의 객체로 취급하게 되는 결과를 가져올 우려가 있으므로, 답변기능을 강화하는 방향으로 개선되어야 한다. 증거조사는 가급적 집중되어야 하며, 증거조사에 관한 현행 형사소송법의 절차는 그대로 준수되어야 한다. 특히 증인신문시 수사기관의 진술과 배치되는 진술을 하는 경우에는 그 신빙성 유무를 면밀하게 따져 보아 법정에서 형성된 심증을 토대로 유무죄를 판단하여야 할 것이다. 무분별한 탄핵증거나 참고자료 등의 제출에 관해서는 보다 엄격한 심사가 필요하며, 양형에 관한 증거조사도 충실하게 할 필요가 있다. 공판중심주의가 법원의 배타적·독선적인 편의주의로 오해되어서는 아니 되며, 법원과 검찰, 변호사 단체가 함께 힘을 모아 더욱 발전해 나가도록 노력해 나가야 할 것이다.
APPLICATIONOFCOMPUTING ANALYSISTO REAL-TIMERAILROAD TRACK INSPECTION (2006)

Prevention offailures intrack hascontinued tobeamajor railroad need. Theproblem is worsening, duetotechnical/cost limitations ininspection technology coupled withcontinuing increase intrack usage/stress. Typically, railroads useanultrasonics, on-track, computer-based, regularly scheduled, real-time inspection. However, thesystemdevelopment emphasis has focused primarily ontheultrasonics, whileincorporating morecomputer science baseddata analysis could helpgaininsight andreduce thetesting errors. Baseduponapplied research atthe University ofConnecticut, together withlocal industry, thispaperdescribes thepattern recognition needs, andproposes aspecific solution approach.
Time‐resolved flow cytometry for the measurement of lanthanide chelate fluorescence: II. Instrument design and experimental results (1994)

A time-resolved flow cytometer capable of measuring a luminescence with a decay time in the range of 10 microseconds to 2 ms, typical for some lanthanide chelates, is presented. The instrument permits acquisition of conventional light scatter and prompt fluorescence signals as well as detection of slowly decaying luminescence by a photon counting unit for a selectable time period of 1 microsecond to 1 ms. During photon counting, the laser beam is turned off by an acoustooptic deflector. The design of a flow chamber with an average geometrical light collection efficiency of 35% over a distance of 1.7 mm is presented and analyzed by ray tracing. A pulse processing system featuring digital integration of the conventional signals and a transputer system for the acquisition and the transfer of the measured parameter values to a host computer is described. Instrument function is verified with lyophilized human lymphocytes stained for the CD8 antigen with dye-loaded liposomes. Quantitation of cell-associated europium chelate fluorescence, displaying a decay time of 1.6 ms, is demonstrated. Elimination of fast decaying background emission generated by DNA-associated ethidium bromide is shown. The background generated by instrument components in the time-gated measurement channel is characterized, and measures for its complete elimination are discussed.
Towards automated calculation of evidence-based clinical scores (2017)

Few clinical scores were deemed "very important" for automated calculation. Future efforts towards score calculator automation should focus on technically feasible "nice to have" scores.
An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery (2016)

Ultrasound is a well-established imaging modality used in clinics worldwide for many different applications. It has also experienced recent growth in interest for therapeutic applications in the form of high-intensity focused ultrasound (HIFU) or focused ultrasound (focused-US), including in capsules for use in drug delivery in the gut. The work described here aimed to support the investigation of its usefulness for drug delivery further. An in vitro sonication system was designed, comprising a focused-US transducer, a cell plate on a vertical stage and transepithelial electrical resistance (TER) meter. Miniature focused-US transducers were fabricated with PZ26 and PZ54 materials (Meggitt Sensing Systems, Kvistgaard, Denmark). The active elements in both transducers have outer diameter, OD = 5 mm, and radius of curvature, R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">C</sub> = 15 mm. US field mapping indicated that output acoustic pressures are in the range 25 kPa <; P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">ac</sub> <; 210 kPa and acoustic powers in the range 8 mW <; Power <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">ac</sub> <; 80 mW. Feasibility testing with Caco-2 colon cancer cells involved measuring TER during and after sonication. Sonication produced a drop in TER of 6.7% after 6 min. Recovery to the starting TER value was observed 14 min after sonication indicating that the sonication system can be used to measure the effect of ultrasound on tissue barrier function as expected.
Severe infective endocarditis with systemic embolism due to community associated methicillin-resistant Staphylococcus aureus ST630 (2014)

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) are increasingly causing infective endocarditis over the past decade. Here we report a healthy man who developed a severe acute infective endocarditis with systemic embolism caused by CA-MRSA. The strain was recovered from repeated blood cultures and was characterized using molecular detection and genotyping. The S. aureus isolate was typed as ST630 SCCmecV with spa-type t4549, agrI/IV and was PVL-negative. This is the only case report, to our knowledge, of CA-MRSA infective endocarditis in China. This case highlights the emergence and geographical spread of life-threatening CA-MRSA infection within China.
Thymidine Phosphorylase: A Possible Marker of Cell Maturation in Acute Myeloid Leukaemia (1980)

Thymidine Phosphorylase activity has been measured in two populations of human Acute Myeloid Leukaemia cells (AML) and in normal human macrophages. During short-term culture in vitro of AML cells the activity of this enzyme increases progressively and approaches that of normal non-dividing macrophages at a time when the leukaemia cells in culture show evidence of maturation as assayed by functional, enzymatic and morphological criteria. It is suggested that the level of Thymidine Phosphorylase activity may be a marker of maturation in AML.
Implementation design for a kernelized trusted DBMS (2003)

The implementation design of the Mitre kernelized trusted database management system (TDBMS) prototype is described. The prototype is intended to be a compartmented-mode DBMS, based on the Naval Surveillance DBMS security model. The TDBMS has been implemented by retrofitting security functionality onto the Mistress commercial database product. The TDBMS security kernel controls read and write access to the database. Mandatory access control is provided down to the data element level of granularity. The prototype supports a set of interactive user-supplied database commands. The TDBMS runs as an application on the prototype Mitre compartment mode workstation (CMW), and has incorporated much of the available CMW security functionality.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Comparative Electrochemical Study of Low Temperature Fluorinated Graphites used as Cathode in Primary Lithium Batteries (2007)

In the present study we show the electrochemical performances of three series of fluorinated graphites CFx-MFn-T used as cathode materials in primary lithium batteries. Li/1M LiClO4 or LiBF4 PC /CFx were cells were discharged at different rates up to 1C. The electrochemical properties are correlated to physico- chemical characterizations. When the fluorine content increases, the capacity increases up to 900 Ah.kg-1. With increased covalency of the C-F bonding, the average discharge potential decreases from 3.15 to 2.31 V. The specific energy peaked at 2277 Wh.kg-1. Thermal storage was performed on cells and capacity losses upon ageing were found satisfactory.
A Case of Intravascular Lymphoma with Mixed Lineage Antigen Expression in a Cat (2007)

A 3-year-old neutered male British shorthair cat was presented to the Clinic for Small Animal Medicine, Internal Medicine and Clinical Pathology, at the Justus-Liebig-University Giessen with a short history of lethargy, severe dyspnoea, fever, anaemia, thrombocytopenia and hyperbilirubinaemia. In blood smears and lymph node aspirates, large pleomorphic lymphatic blasts were detected. On necropsy splenic infarcts and moderate hydrothorax as well as moderate ascites could be observed. Histological examination revealed large numbers of lymphoid blasts within the lumen of the vessels of the lung, lymph nodes, the liver, the chorioid coat of the eyes, in meningeal vessels, arteries and veins of the brain, and within the larger splenic vessels. Neoplastic cells immunohistochemically stained positive for CD45R and CD3. Based on these findings, an intravascular lymphoma was diagnosed.