gebhart/patrepeval huggingface dataset:
Identification of Two Essential Phosphorylated Threonine Residues In the Catalytic Domain of Mekk1 (1997)

The 78-kDa protein kinase Mekk1 plays an important role in the stress response pathway that involves the activation of downstream kinases Sek1 and stress-activated protein kinase/c-Jun NH2-terminal kinase. Conserved serine and threonine residues located between the kinase subdomains VII and VIII of many protein kinases are phosphorylated for maximal kinase activation. Two threonine residues within this region in Mekk1 at positions 560 and 572, but not the serine at 557, were shown to be essential for catalytic activity in this study. When these threonine residues were replaced with alanine, there was a significant loss in phosphotransferase activity toward the primary substrate, Sek1, and a large decrease in autophosphorylation activity. Site-directed mutagenesis demonstrated that these threonine residues cannot be replaced with either serine or glutamic acid for preservation of phosphotransferase activity. Further examination of the Mekk1 mutants isolated from 32P-labeled transfected COS cells showed that Thr-560 and Thr-572 were indeed phosphorylated after two-dimensional tryptic-chymotryptic phosphopeptide analysis. Additional determinants in the NH2-terminal domain of Mekk1 also play a role in the regulation of Mekk1 activity. Although Pak3 and PKC can activate Mekk1 in vivo, this interaction is indirect and independent, since there was no direct phosphorylation of Mekk1 by Pak3 or PKC or of Pak3 by PKC, respectively.
High Resolution (Semi-)Automatic Determination of Microseismic Event Locations (2003)

P181 HIGH RESOLUTION (SEMI-)AUTOMATIC DETERMINATION OF MICROSEISMIC EVENT LOCATIONS 1 Introduction 1 K. DE MEESMAN 1 M. VAN DER BAAN 1 J-M. KENDALL 1 and R.H. JONES 2 1 School of Earth Sciences University of Leeds Leeds LS2 9JT UK 2 ABB Offshore Systems Rosemanowes TR10 9DU UK Passive seismic monitoring is becoming a powerful tool for managing and studying hydrocarbon reservoirs. The data provides information on the reservoir’s seismic anisotropy the hydraulic properties of reservoir and fractures and it can pinpoint regions of changing pressure conditions [Jupe et al. 2000]. Crucial for retrieving this information are the locations of
MYOCARDIAL NFKB IS RAPIDLY ACTIVATED FOLLOWING LPS OR TNFA CHALLENGE (1999)

Society Of Critical Care Medicine 29Th Educational And Scientific Symposium; Orlando, Florida, Usa; February 11-15, 2000: Oral Presentations
Tribbles-1 as a Novel Biomarker of Chronic Antibody-Mediated Rejection (2008)

Diagnosis of the specific cause of late allograft injury is necessary if more personalized and efficient immunosuppressive regimens are to be introduced. This study sought previously unrecognized biomarkers for specific histologic diagnoses of late graft scarring by comparison of gene sets from published microarray studies. Tribbles-1 (TRIB1), a human homolog of Drosophila tribbles, was identified to be a potentially informative biomarker. For testing this, mRNA expression in 76 graft biopsies, 71 blood samples, and 11 urine samples were profiled from independent cohorts of renal transplant patients with different histologic diagnoses recruited at two European centers. TRIB1 but not TRIB2 or TRIB3 was found to be a potential blood and tissue biomarker of chronic antibody-mediated rejection, an active immune-mediated form of chronic allograft failure associated with a poor prognosis. TRIB1 mRNA levels in peripheral blood mononuclear cells discriminated patients with chronic antibody-mediated rejection from those with other types of late allograft injury with high sensitivity and specificity. TRIB1 was also upregulated in a rodent model of chronic cardiac vasculopathy, suggesting that this biomarker may be useful in other solid-organ transplants and across species. It was determined that TRIB1 is expressed primarily by antigen-presenting cells and activated endothelial cells. Overall, these data support the potential use of TRIB1 as a biomarker of chronic antibody-mediated allograft failure.
Robust control design for a wheel loader using mixed sensitivity h-infinity and feedback linearization based methods (2005)

The existing industry practices for the design of control systems in construction machines primarily rely on classical designs coupled with ad-hoc synthesis procedures. Such practices lack a systematic procedure to account for invariably present plant uncertainties in the design process as well as coupled dynamics of the multi-input multi-output (MIMO) configuration. In this paper, an H/sub /spl infin// based robust control design combined with feedback linearization is presented for an automatic bucket leveling mechanism of a wheel loader. With the feedback linearization control law applied, stability robustness is improved. A MIMO nonlinear model for an electro-hydraulically actuated wheel loader is considered. The robustness of the controller designs are validated by using analysis and by simulation using a complete nonlinear model of the wheel loader system.
Challenges and Future of Enterprise Application Integration (2012)

In today's rapid growing business world, organizations are relying on technology and need of the integration of the disparate applications is in high demand.
Safety, tolerability, and immune-biomarker profiling for year-long sargramostim treatment of Parkinson's disease (2021)

The research was supported by community funds to the University of Nebraska Foundation and federal research support from 5 R01NS034239-25.
Unsupervised Reconstruction of Sea Surface Currents from AIS Maritime Traffic Data Using Trainable Variational Models (2021)

The estimation of ocean dynamics is a key challenge for applications ranging from climate modeling to ship routing. State-of-the-art methods relying on satellite-derived altimetry data can hardly resolve spatial scales below ∼100 km. In this work we investigate the relevance of AIS data streams as a new mean for the estimation of the surface current velocities. Using a physics-informed observation model, we propose to solve the associated the ill-posed inverse problem using a trainable variational formulation. The latter exploits variational auto-encoders coupled with neural ODE to represent sea surface dynamics. We report numerical experiments on a real AIS dataset off South Africa in a highly dynamical ocean region. They support the relevance of the proposed learning-based AIS-driven approach to significantly improve the reconstruction of sea surface currents compared with state-of-the-art methods, including altimetry-based ones.
Molecular Cloning and Baculovirus Expression of the Rabbit Corneal Aldehyde Dehydrogenase (ALDH1A1) cDNA (2003)

Most mammalian species express high concentrations of ALDH3A1 in corneal epithelium with the exception of the rabbit, which expresses high amounts of ALDH1A1 rather than ALDH3A1. Several hypotheses that involve catalytic and/or structural functions have been postulated regarding the role of these corneal ALDHs. The aim of the present study was to characterize the biochemical properties of the rabbit ALDH1A1. We have cloned and sequenced the rabbit ALDH1A1 cDNA, which is 2,073 bp in length (excluding the poly(A+) tail), and has 5' and 3' nontranslated regions of 46 and 536 bp, respectively. This ALDH1A1 cDNA encodes a protein of 496 amino acids (Mr = 54,340) that is: 86-91% identical to mammalian ALDH1A1 proteins, 83-85% identical to phenobarbital-inducible mouse and rat ALDH1A7 proteins, 84% identical to elephant shrew ALDH1A8 proteins (eta-crystallins), 69-73% identical to vertebrate ALDH1A2 and ALDH1A3 proteins, 65% identical to scallop ALDH1A9 protein (omega-crystallin), and 55-57% to cephalopod ALDH1C1 and ALDH1C2 (omega-crystallins). Recombinant rabbit ALDH1A1 protein was expressed using the baculovirus system and purified to homogeneity with affinity chromatography. We found that rabbit ALDH1A1 is catalytically active and efficiently oxidizes hexanal (Km = 3.5 microM), 4-hydroxynonenal (Km = 2.1 microM) and malondialdehyde (Km = 14.0 microM), which are among the major products of lipid peroxidation. Similar kinetic constants were observed with the human recombinant ALDH1A1 protein, which was expressed and purified using similar experimental conditions. These data suggest that ALDH1A1 may contribute to corneal cellular defense against oxidative damage by metabolizing toxic aldehydes produced during UV-induced lipid peroxidation.
Robust estimation of the noise variance from background MR data (2006)

In the literature, many methods are available for estimation of the variance of the noise in magnetic resonance (MR) images. A commonly used method, based on the maximum of the background mode of the histogram, is revisited and a new, robust, and easy to use method is presented based on maximum likelihood (ML) estimation. Both methods are evaluated in terms of accuracy and precision using simulated MR data. It is shown that the newly proposed method outperforms the commonly used method in terms of mean-squared error (MSE).
Diffusion model to describe osteogenesis within a porous titanium scaffold (2015)

In this study, we develop a two-dimensional finite element model, which is derived from an animal experiment and allows simulating osteogenesis within a porous titanium scaffold implanted in ewe's hemi-mandible during 12 weeks. The cell activity is described through diffusion equations and regulated by the stress state of the structure. We compare our model to (i) histological observations and (ii) experimental data obtained from a mechanical test done on sacrificed animal. We show that our mechano-biological approach provides consistent numerical results and constitutes a useful tool to predict osteogenesis pattern.
A gibberellin‐regulated xyloglucan endotransglycosylase gene is expressed in the endosperm cap during tomato seed germination (2002)

Xyloglucan endotransglycosylases (XETs) modify xyloglucans, major components of primary cell walls in dicots. A cDNA encoding an XET (LeXET4) was isolated from a germinating tomato (Lycopersicon esculentum Mill.) seed cDNA library. DNA gel blot analysis showed that LeXET4 is a single-copy gene in the tomato genome. LeXET4 mRNA was strongly expressed in germinating seeds, was much less abundant in stems, and was not detected in roots, leaves or flower tissues. During germination, LeXET4 mRNA was detected in seeds within 12 h of imbibition with maximum mRNA abundance at 24 h. Tissue prints showed that LeXET4 mRNA was localized exclusively to the endosperm cap region. Expression of LeXET4 was dependent on exogenous gibberellin (GA) in GA-deficient (gib-1 mutant) tomato seeds, while abscisic acid, a seed germination inhibitor, had no effect on LeXET4 mRNA expression in wild-type seeds. LeXET4 mRNA disappeared after radicle emergence, even though degradation of the lateral endosperm cell walls continued. The temporal, spatial and hormonal regulation pattern of LeXET4 gene expression suggests that XET has a role in endosperm cap weakening, a key process regulating tomato seed germination.
Synthetic Procedure for Various Selenium-Containing Electron Donors of the Bis(Ethylenedithio)tetrathiafulvalene (BEDT-TTF) Type (2002)

Six selenium variants of BEDT-TTF have been successfully synthesized by a newly developed synthetic method that involves a combination of two key reactions for the construction of two kinds of heterocyclic rings: the first is a one-pot formation of 1,3-dichalcogenole-2-chalcogenones from a common starting material, THP-protected 2-(ethynylthio)ethanol, leading to the inner five-membered rings, and the other is the annelation of the outer six-membered heterocyclic ring onto the inner ring by an intramolecular transalkylation reaction on a chalcogen atom. This method turned out to be widely applicable to the syntheses of the electron donors of bis(ethylenedithio)- and bis(ethyleneselenothio)-substituted types. However, synthetic attempts to form analogous donors of the bis(ethylenediseleno)-substituted type from THP-protected 2-(ethynylseleno)ethanol were unsuccessful. This is attributable to the predominance of side-reactions via a seleniranium (episelenonium) salt over the desired six-membered ring formation by transalkylation via a seleninium salt.
Ions in the Brain: Normal Function, Seizures, and Stroke (2004)

PART I: IONS AND WATER IN THE BRAIN 1. The blood-brain barrier 2. The regulation of brain ions 3. Osmotic stress and the brain 4. The influence of ion concentrations on neuron function PART II: CEREBRAL SEIZURES 5. A primer of epileptiform seizures 6. Experimental seizures 7. Electrophysiology of seizures 8. Ions and seizures 9. Solving seizure mechanisms by simulations 10. Seizures, channels, genes 11. Mechanisms of action of antiepileptic drugs PART III: SPREADING DEPRESSION OF LEAO 12. Introduction to spreading depression: Extracellular electric signs 13. Ion fluxes during spreading depression 14. Membrane potential and input resistance during spreading depression 15. The mechanism of spreading depression 16. Solving the puzzle of spreading depression by computer simulation PART IV: PATHOLOGICAL BIOPHYSICS OF CEREBRAL HYPOXIA AND STROKE 17. Hypoxia, asphyxia and ischemia 18. Mechanism of early, reversible hypoxic synaptic failure 19. Irreversible hypoxic (ischemic) neuron injury
Larger Acetabular Labrum Is Associated With Hip Dysplasia, Joint Incongruence, and Clinical Symptoms (2020)

Level Ⅳ, retrospective cross-sectional study.
Gold nanoparticle bioconjugates labelled with 211At for targeted alpha therapy (2017)

Gold nanoparticles labeled with <sup>211</sup>At are very effective in radionuclide therapy.
A novel process-variation insensitive network for on-chip impedance matching (2005)

Impedance matching networks are used widely in RF circuit design for many purposes such as maximum power transfer or optimal noise match. Passive impedance matching networks involving capacitors and inductors are often employed, but they are subject to process variation. The paper presents a process-variation insensitive network with matched passive components that has a low sensitivity to process variation. This network,, when applied in impedance matching, can lead to a reliable network with high immunity to process variation. The improvement on immunity as compared to a conventional passive network is revealed in Monte Carlo simulation. This approach can be extended to broadband impedance matching by cascading the proposed network.
Nonlinear low-to-high-frequency energy cascades in diatomic granular crystals (2015)

We study wave propagation in strongly nonlinear one-dimensional diatomic granular crystals under an impact load. Depending on the mass ratio of the "light" to "heavy" beads, this system exhibits rich wave dynamics from highly localized traveling waves to highly dispersive waves featuring strong attenuation. We demonstrate experimentally the nonlinear resonant and antiresonant interactions of particles, and we verify that the nonlinear resonance results in strong wave attenuation, leading to highly efficient nonlinear energy cascading without relying on material damping. In this process, mechanical energy is transferred from low to high frequencies, while propagating waves emerge in both ordered and chaotic waveforms via a distinctive spatial cascading. This energy transfer mechanism from lower to higher frequencies and wave numbers is of particular significance toward the design of novel nonlinear acoustic metamaterials with inherently passive energy redistribution properties.
Referenceless phase velocity mapping using balanced SSFP (2009)

Phase contrast MRI (PC-MRI) is an established technique for measuring blood flow velocities in vivo. Although spoiled gradient recalled echo (GRE) PC-MRI is the most widely used pulse sequence today, balanced steady state free precession (SSFP) PC-MRI has been shown to produce accurate velocity estimates with superior SNR efficiency. We propose a referenceless approach to flow imaging that exploits the intrinsic refocusing property of balanced SSFP, and achieves up to a 50% reduction in total scan time. With the echo time set to exactly one half of the sequence repetition time (TE = TR/2), we show that non-flow-related image phase tends to vary smoothly across the field-of-view, and can be estimated from static tissue regions to produce a phase reference for nearby voxels containing flowing blood. This approach produces accurate in vivo one-dimensional velocity estimates in half the scan time compared with conventional balanced SSFP phase-contrast methods. We also demonstrate the feasibility of referenceless time-resolved 3D flow imaging (called "7D" flow) in the carotid bifurcation from just three acquisitions.
Receptor protein tyrosine phosphatase mu expression as a marker for endothelial cell heterogeneity; analysis of RPTPmu gene expression using LacZ knock-in mice (2003)

The receptor-like protein tyrosine phosphatase mu (RPTPmu) belongs to the subfamily of meprin, A5, RPTPmu (MAM) domain-containing RPTPs, which are thought to play an important role in cell-cell adhesion mediated processes. The current study was designed to examine the expression pattern of RPTPmu in mice. We have generated RPTPmu-LacZ knock-in mice that express the beta-galactosidase (LacZ) reporter gene under the control of the RPTPmu promoter. LacZ expression patterns were analysed in embryos and adult mice by whole mount LacZ staining. Analysis of beta-galactosidase activity of heterozygous embryos and adult tissues revealed RPTPmu expression in endothelial cells of arteries and capillaries. In contrast, expression was virtually absent in endothelial cells of veins and in fenestrated endothelial cells in the adult liver and spleen. Moreover, RPTPmu expression was found in endothelial cells from the endocardium and the aorta in embryos, but not in adult mice. In addition to heterogeneous expression in endothelial cells, RPTPmu expression was found in cardiac muscle cells but not in skeletal muscle cells or smooth muscle cells. Expression was also found in Type II pneumonocytes in the lung alveoli and in Purkinje cells and other neurons in the brain. The specific expression of RPTPmu in arterial endothelial cells and in cardiac myocytes suggests that RPTPmu may play a role in the regulation of cardiovascular functions.
Mechanical Properties of an Fe‐Based SAM2×5‐630 Metallic Glass Matrix Composite with Tungsten Particle Additions (2018)

We present the role of tungsten additions on the mechanical properties of a Fe‐based structural amorphous metal (SAM2×5‐630) containing crystalline tungsten. Matrix cracking by microindentation is inhibited by the addition of tungsten and indicates that tungsten improves the fracture toughness. Response surfaces from nanoindentation arrays indicate that the hardness and modulus of the matrix phase are increased by tungsten additions. Bulk composites with 30 vol% tungsten subjected to 4‐point flexure exhibited brittle fracture behavior and the characteristic strength and Weibull modulus were 165 and 8.7 MPa, respectively. The addition of tungsten did not cause devitrification of the matrix phase.
Thermal Analysis of a TREAT Fuel Assembly (2014)

The objective of this study was to explore options as to reduce peak cladding temperatures despite an increase in peak fuel temperatures. A 3D thermal-hydraulic model for a single TREAT fuel assembly was benchmarked to reproduce results obtained with previous thermal models developed for a TREAT HEU fuel assembly. In exercising this model, and variants thereof depending on the scope of analysis, various options were explored to reduce the peak cladding temperatures.
An algorithm for associating the features of two images (1991)

In this paper we describe an algorithm that operates on the distances between features in the two related images and delivers a set of correspondences between them. The algorithm maximizes the inner product of two matrices, one of which is the desired 'pairing matrix' and the other a 'proximity matrix' with elements exp (-rij2/2 sigma 2), where rij is the distance between two features, one in each image, and sigma is an adjustable scale parameter. The output of the algorithm may be compared with the movements that people perceive when viewing two images in quick succession, and it is found that an increase in sigma affects the computed correspondences in much the same way as an increase in interstimulus interval alters the perceived displacements. Provided that sigma is not too small the algorithm will recover the feature mappings that result from image translation, expansion or shear deformation--transformations of common occurrence in image sequences--even when the displacements of individual features depart slightly from the general trend.
Survival and reproduction of enchytraeid worms, Oligochaeta, in different soil types amended with energetic cyclic nitramines (2005)

Abstract Hexanitrohexaazaisowurtzitane (CL-20), a new polycyclic polynitramine, has the same functional nitramine groups (N-NO2) as the widely used energetic chemicals hexahydro-1,3,5-trinitro-1,3,5-triazacyclohexane (royal demolition explosive [RDX]) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (high-melting explosive [HMX]). Potential impacts of CL-20 as an emerging contaminant must be assessed before its use. The effects of CL-20, RDX, or HMX on adult survival and juvenile production by potworms Enchytraeus albidus and Enchytraeus crypticus were studied in three soil types, including Sassafras sandy loam (1.2% organic matter [OM], 11% clay, pH 5.5), an agricultural soil (42% OM, 1% clay, pH 8.2), and a composite agricultural-forest soil (23% OM, 2% clay, pH 7.9) by using ISO method 16387 (International Standard Organization, Geneva, Switzerland). Results showed that CL-20 was toxic to E. crypticus with median lethal concentration values for adult survival ranging from 0.1 to 0.7 mg/kg dry mass (DM) when using the three tested soils. In addition, CL-20 adversely affected juvenile production by both species in all soils tested, with median effective concentration (EC50) values ranging from 0.08 to 0.62 mg/kg DM. Enchytraeus crypticus and E. albidus were similarly sensitive to CL-20 exposure in the composite agricultural-forest soil, which supported reproduction by both species and enabled comparisons. Correlation analysis showed weak or no relationship overall among the soil properties and reproduction toxicity endpoints. Neither RDX nor HMX affected (p &amp;gt; 0.05) adult survival of either species below 658 and 918 mg/kg DM, respectively, indicating that CL-20 is more toxic to enchytraeids than RDX or HMX. Examination of data shows that CL-20 should be considered as a potential reproductive toxicant to soil invertebrates, and that safeguards should be considered to minimize the potential for release of CL-20 into the environment.
Power Management for Hospital Combined Distributed Power with Load Prediction Using Deep Learning in Islanded Operation Mode (2020)

The purpose of this paper is to propose a power management method in a hospital for a combination of emergency generators (EGs) with photovoltaic power generation (PV). The power balance of the grid not only influences the droop control for the generator but also the output fluctuations of the PV. Frequency control and a load control by a load prediction are necessary for the system grid combined with EGs and PV in an islanded operation mode. When the PV system is installed in the grid, the EG system should distribute power to small generators, the reason is because when the EG is too large, the power balance cannot be maintained to stabilize the system frequency in all power ranges. Since the distributed generation system needs the demand for each generator, it is important to predict the load. This paper proposes a new method for power energy management for stabilization with the islanded operation mode in a hospital power grid with load prediction using deep learning. The proposed method can realize operation by using a power emulator with the hospital power grid model. The verification of results show that the power emulator is effective in the energy management strategies.