gebhart/patrepeval huggingface dataset:
Abstract 2786: Potent inhibition of human liposarcoma cell growth and survival by novel modulators of the MDM2-p53 interaction (2012)

Abstract The tumor suppressor p53 is commonly inactivated in human malignancies. One mechanism is by genomic amplification and consequent overexpression of the ubiquitin E3 ligase MDM2, which targets p53 for proteosome-mediated degradation. The MDM2 gene is amplified in &amp;gt;95% of human liposarcomas, a disease for which the efficacy of systemic therapies is limited. Here, we compared the effects of two novel highly potent and selective inhibitors of the MDM2-p53 interaction, with the classic MDM2 inhibitor Nutlin-3 on the expression and activity of p53 and the resulting changes in human liposarcoma cell proliferation and survival. We determined MDM2 copy number, p53 mutational status, and relative expression levels of MDM2 and p53 proteins in 8 human liposarcoma cell lines and normal human preadipocytes/adipocytes. TP53 status was wild type in 7 of 8 liposarcoma cell lines, which also had genomic amplification of MDM2. MDM2 protein expression correlated with the degree of amplification and was highly expressed in comparison to normal preadipocytes/adipocytes. The two spiro-oxindole derivatives - Compounds A and B- (1) and Nutlin-3 were applied to liposarcoma cell lines with or without p53 siRNA and the biological consequences were determined by immunoblot, cell viability, cell cycle and apoptosis assays. Treatment with MDM2 inhibitors led to increased p53 protein levels and induction of its transcriptional targets MDM2 and p21. Cell viability was markedly decreased in a dose-dependent manner in liposarcoma cells with wild-type p53, with IC50 values of 0.40-4.23 μM, 0.37-1.88 μM and 2.72-9.83 μM for Compound A, Compound B, and Nutlin-3, respectively. These effects were markedly abrogated by siRNA-mediated p53 knockdown and in a cell line harboring a de novo p53 mutation. Compound A and Compound B also induced cell cycle arrest and apoptosis at approximately 5 fold lower concentrations than Nutlin-3. At low concentrations, G0/G1 cell arrest occurred, whereas higher concentrations resulted in G2/M arrest and induction of apoptosis. Cell line and tumor xenograft experiments are ongoing. These data demonstrate that Compounds A and B are highly potent and specific inhibitors of MDM2 with activity in genetically characterized liposarcoma cells. Further study and clinical development in this disease is warranted. 1. Wang S. et al., AACR, Orlando FL, 2011, Abstract LB-204 Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2786. doi:1538-7445.AM2012-2786
TRIPLE - an RDF Rule Language with Context and Use Cases (2005)

TRIPLE was designed as a practical rule language for data manipulation applications. Over the last couple of years the language has been deployed in various applications and use case studies. In this paper we first introduce the design principles of TRIPLE and then present some of the applications for which this language has been used.
Taking the “F” out of forever chemicals (2022)

The right solvent mix breaks down perfluorinated organic acids.
Classification of the nonlinear dynamics in an initially curved bistable micro/nano‐electro‐mechanical system resonator (2015)

The nonlinear dynamics of a bistable micro/nano‐electro‐mechanical system resonator composed of an arch‐shaped microbeam is investigated. The initially curved microbeam is actuated through a combined DC and AC electrostatic parallel plate field. A single degree of freedom model obtained using the Galerkin's decomposition method with distributed electrostatic force is implemented in order to investigate the resonator dynamics near its primary resonance. According to the shape of the potential energy function which depends on the system parameters, the nonlinear dynamics of the system are classified into certain categories. The appearance of various nonlinear phenomena including dynamic snap‐through, dynamic pull‐in, chaotic or large amplitude vibrations, hysteresis and softening‐type behaviours are discussed within the introduced categories. A typical case scrutinised on detail, showing consequent snap‐through instabilities which are responsible for transitions between the present stable configurations of the arch‐shaped microbeam. Details of the resulting hysteresis loop governing these transitions are discussed. Moreover, discussion is provided about the formation of the hysteresis loops which can affect the filtering functionality of the proposed bistable MEMS resonator.
Longitudinal micro-CT-derived biomarkers: the new standard readouts for preclinical evaluation of pulmonary fibrosis and therapy (2019)

Pulmonary fibrosis is life-threatening and effective treatment is still lacking. To improve translation of mouse models to man, we introduced an in vivo µCT protocol to repeatedly assess lung fibrosis progression in a silica-induced mouse model. Mice were endotracheally instilled with crystalline silica or saline and scanned weekly for 15 weeks with respiratory gated high-resolution low radiation-dose µCT. We evaluated the disease onset and progress, using biomarkers derived from the scans. At day 7, 35, 63 and 105 after instillation, imaging data were cross-validated with lung function, cellular, histochemical, molecular and histological readouts. Silica instillation resulted in a significant and progressive increase of non-aerated lung volume (corresponding with inflammatory and fibrotic processes) and total lung volume (corresponding to compensatory host response). The aerated lung volume transiently decreased to increase again, indicating an acute inflammatory response. Imaging results were confirmed by a significantly decreased Tiffeneau index (d35 and d63), increased neutrophilic inflammation (d7 - d105), increased IL-13, MCP-1, MIP-2 and TNF-α concentration in BAL fluid (d7 - d105), increased collagen content (d35 - d105) and granuloma formation (d35 - 105). Our findings indicate that µCT is an excellent technique to longitudinally evaluate pulmonary fibrosis, a novel approach that allows assessment of disease progression and stabilization over several weeks. This will facilitate the evaluation of therapeutic interventions at the level of the individual animal.
Investigation of Inherent Transient Recovery Voltage for Generator Circuit Breakers (1983)

The transient recovery voltage (TRV) for generator circuit breakers is investigated. The TRV for fault currents from the generator as well as from the system through the generator step-up transformers is calculated: A generalized expression for the TRV is developed and dimensionless plots made. Test results on two generating units are presented, showing good agreement with calculated values. A basis of rating for generator breaker TRV is also presented. TRV waveshapes and TRV parameters are suggested as part of the basis of rating.
Intraoperative control of extracranial—intracranial bypass patency by near-infrared indocyanine green videoangiography (2005)

Indocyanine green videoangiography provides a reliable and rapid intraoperative assessment of bypass patency. Thus, ICG videoangiography may help reduce the incidence of early bypass graft failure.
Phosphoribosylpyrophosphate synthetase (PrsA) variants alter cellular pools of ribose 5-phosphate and influence thiamine synthesis in Salmonella enterica (2010)

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. This supports our principal goal to develop, expand and strengthen the networks available to our members so that they can generate new knowledge about microbes and ensure that it is shared with other communities.
Unexpected arterial wall and cellular inflammation in patients with rheumatoid arthritis in remission using biological therapy: a cross-sectional study (2016)

A subset of patients with RA in clinical remission have activated monocytes and increased inflammation in the arterial wall, despite the use of potent TNF blocking therapies. In these subjects, RA disease duration was the most important contributor to the level of arterial wall inflammation. This increased inflammatory state implies higher cardiovascular risk in these patients, who thus may require more stringent CV risk management.
The effect of sodium hexafluoroantimonate on the properties of the conversion coatings formed by plasma electrolytic oxidation of a magnesium alloy Wpływ heksafluoroantymonianu sodu na właściwości powłok konwersyjnych wytworzonych podczas elektrolitycznego utleniania plazmowego stopu magnezu (2016)

Wydawnictwo SIGMA-NOT wydaje czasopisma fachowe informujące swoich czytelników o najnowszych osiągnięciach naukowych i nowoczesnych rozwiązaniach technicznych w Polsce i na świecie, popularyzuje problemy techniczne oraz poszerza wiedzę i kulturę techniczną.
Effect of intraocular pressure on uveoscleral outflow following cyclodialysis in the monkey eye. (1985)

Cyclodialysis was performed in both eyes of five cynomolgus monkeys. Two days later the intraocular pressure (IOP) had fallen from 17.7 +/- 0.8 to 7.1 +/- 1.4 mm Hg (P less than 0.001). At that time, both eyes were perfused for 30 min with fluorescein-isothiocyanate (FITC) dextran (MW 70,000), one at 35 mm Hg and the other at 4 mm Hg. Four pairs of control eyes (without cyclodialysis) were perfused in the same manner. At 4 mm Hg, uveoscleral outflow was 0.02 +/- 0.02 microliter/min in control eyes and 0.05 +/- 0.04 microliter/min in eyes following cyclodialysis. However, at 35 mm Hg, uveoscleral outflow in eyes with cyclodialysis increased to 2.13 +/- 0.47 microliters/min compared to 0.32 +/- 0.10 microliter/min in control eyes. Thus the "facility" of uveoscleral outflow in control eyes is 0.01 microliter/min/mm Hg and in eyes following cyclodialysis is 0.07 microliter/min/mm Hg. It is concluded that cyclodialysis results in a pressure-dependent increase in uveoscleral outflow.
Preparation of microsieves - using sacrificial temp lates (2009)

Microsieves Filtration membranes bearing dense arrays of uniform pores of sub-micrometric diameter and having a thickness smaller than the pore size were first introduced by Cees van Rijn in 1995.[1] Compared to conventional filtration media they offer a lower flow resistance [2], a smaller tendency of fouling and a sharper size selectivity. Usually, microsieves are prepared from inorganic materials using photolithography. The use of inorganic material facilitates cleaning and provides rigidity, the use of photolithography allows precise control of the size and arrangement of the pores and the preparation of hierarchically structured microsieves. [3] Here we show an alternative route for the production of microsieves: sacrificial templates (particles[4], water droplets[5]) are applied to liquid or solid surfaces, embedded in a liquid wetting layer that subsequently is solidifies either via vitrification or photopolymerization and separated from the solid substrate. Finally, the sacrificial templates are removed to yield a microsieve composed of an organic polymer.
On-chip microreactor system for the production of nano-emulsion loaded liposomes: towards targeted delivery of lipophilic drugs (2011)

An on-chip microreactor system for the production of novel nano-biodevices is presented. This nano-biodevice consists of a nano-emulsion loaded with lipophilic drugs, entrapped in liposomes. These nano-biodevices can be equipped with targeting molecules for higher drug efficiency. The microreactor system enables efficient production of the complicated nano-biodevices. We used the anti-inflammatory drug celecoxib dissolved in fish oil for the nanoemulsion. The nano-emulsion is stabilized with a surfactant dissolved in water. The microreactor provides great flexibility, and therefore the nano-biodevices can be produced at an appropriate location and can be customized for the patient.
Reduction of Indigo: Simple Syntheses of 3-Acetoxy-, 1-Acetyl-2,3-dihydro-, 3-Acetoxy-3'-acetyl-, 3-Acetoxy-1,3'-diacetyl-2,2'-bisindoles, and 2,2'-Bisindole (1997)

Indigo was converted to 2,Z-bisindole by the direct re duction with zinc in acetic acid and acetic anhydride under argon or hydrogen atmosphere.Reduction with tin and iron afforded 3-acetoxy-2,Z-bisindole predominantly.Useful building blocks such as I-acetyl-2,3-dihydro-, 3-acetoxy-3'-acetyl-, and 3-acetoxy-13-diacetyl-2.2'-bisindoles were also produced depending on metal and reaction conditions.lndigo (1 ) has been used as the oldest known coloring maner.2The dyeing technique is based on such chemical reactions that 1 is transformed to leucoindigo (2) with proper reducing agents and2 regenerates 1 by oxidation with oxygen on standing (Scheme 1).Various anempts for reduction of 1 ware d o c ~r n e n t e d , ~. ~ but formation of other products except 2 was not reported.Trapping of desoxyindigo as 3-acetoxy-2,Y-bisindole P) by Bergman and c o -~o r k e f f i ~* ~ was the only successful result thus far reported, though the yield was 19% yield.In our synthetic projectlb* directed toward indolo[2.3-a]pyrrolo[3.4-c]carbazole-5.7(6-dionederivat i v e ~~~* ~, ~ (4), we have developed a synthesis of 2,Z-bisindole (5) as a key intermediate through an oxidative coupling of 2-lithio-1-methoxyindole5 (6) followed by catalytic hydrogenation over 10% PdIC.Our need for the simpler synthesis of 5 has led us to examine the direct reduction of 1 expecting production of 5 and other related 2,Z-bisindole derivatives in spite of the above discouraging literatures.2-4Now, we discovered a simple synthesis of either 3-acetoxy-(3), 1 -acetyi-2,3-dihydro-v), 3-acetoxy-3'-acetyl-(a), 3-acetoxy-1.3'-diacetyl-22bisindoles(9).or 2.2'-bisindole (5).We have found that acid sensitive 5 is tolerable to AcOH.Considering the fact and the nature of 2 suceptible to oxidation, we planned toconvert2, immediately as it is generated in a reaction medium, to the more easily reducible diacetyl compound to promote further reduction.Based on the idea, we tried direct reduction of 1
Isolierung und Charakterisierung der homoleptischen NiI und NiII Bis(Benzol)‐Sandwich Kationen (2022)

Abstract Ein stabiles Salz des in der kondensierten Phase bisher unbekannten Metalloradikals [Ni(C 6 H 6 ) 2 ] + wurde aus [Ni(CO) 4 ] + [WCA] − und Benzol synthetisiert ([WCA] − =[F{Al(OR F ) 3 } 2 ] − ; R F =C(CF 3 ) 3 ). Die Kristallstruktur zeigt eine bemerkenswerte asymmetrische η 3 ,η 6 ‐Sandwich‐Struktur. Die magnetischen Eigenschaften des [Ni(C 6 H 6 ) 2 ] + ‐Kations wurden in Lösung und in der Gasphase untersucht. Die Oxidation mit dem synergistischen Ag + /0.5 l 2 ‐System führte zu dem Salz [Ni(C 6 H 6 ) 2 ] 2+ ([WCA] − ) 2 . Alle Produkte wurden mittels IR, Raman, NMR/EPR, Einkristall‐ und Pulver‐XRD vollständig charakterisiert.
Live web search experiments for the rest of us (2010)

There are significant barriers to academic research into user Web search preferences. Academic researchers are unable to manipulate the results shown by a major search engine to users and would have no access to the interaction data collected by the engine. Our initial approach to overcoming this was to ask participants to submit queries to an experimental search engine rather than their usual search tool. Over several different experiments we found that initial user buy-in was high but that people quickly drifted back to their old habits and stopped contributing data. Here, we report our investigation of possible reasons why this occurs. An alternative approach is exemplified by the Lemur browser toolbar, which allows local collection of user interaction data from search engine sessions, but does not allow result pages to be modified. We will demonstrate a new Firefox toolbar that we have developed to support experiments in which search results may be arbitrarily manipulated. Using our toolbar, academics can set up the experiments they want to conduct, while collecting (subject to human experimentation guidelines) queries, clicks and dwell times as well as optional explicit judgments.
A workload-aware-design of 3D-NAND flash memory for enterprise SSDs (2014)

Solid-state drives (SSDs) have a growing trend of replacing hard disk drives (HDDs) in large computing systems to meet the requirements of power and space. Data in SSD are stored in NAND flash memory cells. Since 2-dimensional (2D) scaling is facing various limitations, 3D-NAND flash memory architectures have been proposed to maintain the trend of bit density increase and bit cost reduction, which prefers large block sizes and page sizes. However, overly large block and page sizes flash memory harm the throughput of NAND flash devices. The actual page size and block size of the NAND flash memory product are as small as 8KB and 2MB respectively to avoid the performance degradation. Alternatively, emerging nonvolatile memory devices named storage class memories (SCMs) feature in high speed, low power consumption and high endurance. By combining SCM, large block sizes and page sizes, required especially for the 3D-NAND flash device case, are acceptable for SCM/NAND flash hybrid SSD. In this paper, a workload-aware NAND organization design is investigated for enhancing the performance of both SSD with only NAND flash memory and SCM/NAND flash hybrid SSD. From the experimental results, a 16MB NAND block size that corresponds to 512 layers and 16KB page size in a 512Gbit P-BiCS 3D-NAND flash memory can be acceptable for applications like relational database and financial online transaction processing. Additionally, a large NAND flash page size of 512KB is also acceptable for the firewall/web proxy, relational database and project directories applications. With SCM, the acceptable page and block sizes of the 3D-NAND flash memory can be magnified up to 64-times and 4-times, respectively, compared with the conventional SSD composed of only NAND flash memory.
New Topical Agents for Treatment of Partial-thickness Burns in Children: A Review of Published Outcome Studies. (2008)

﻿ Evidence-based choices for treating burns in children are not well defined. Skin substitutes and contemporary dressings offer potential advantages over traditional treatment with topical antimicrobial agents in treating partial-thickness burns. Newer treatment modalities may reduce morbidity, financial burdens, and scarring by accelerating healing. Reports of pediatric burn management from 1997 to 2007 were reviewed to compare agent performance with outcome measures such as healing time, pain moderation, cosmetic results, and hospital costs. Transcyte™ (Smith & Nephew, London), Biobrane® (Bertek Pharmaceuticals Inc, Morgantown, WV), beta-glucan collagen, and Mepitel® (Mölnlycke, Göteborg, Sweden) have been reported as superior to silver sulfadiazine (SSD) in achieving faster healing times and decreased pain in pediatric patients. Initial reports describing the outcomes achieved with these new agents indicate that they may offer clinical advantages in the treatment of partial-thickness burns in children. Increased costs of the new products appeared to be offset by decreases in hospital stay, nursing care time and pain medications. The existing literature is not conclusive, and prospective trials with standardized outcome measures are needed to better define the role of these agents. ﻿﻿﻿.
FET-based planar circuits for quasi-optical sources and transceivers (1989)

The circuits that are simple in construction and exhibit isotropic conversion gain are discussed. Both single-device and balanced transmitter and transceiver circuits are described. Balanced sources are presented that can be operated in push-pull mode for frequency doubling. A single-FET quasi-optical oscillator circuit using a microstrip patch linear array and having an effective radiated power of 31.6 dBm at 10 GHz is reported. A dual-FET frequency-doubling oscillator circuit using a coupled rampart line antenna with an effective radiated power of 21.6 dBm at 19.7 GHz is also described. Quasi-optical transceiver elements are presented that use the FETs as both signal sources and self-oscillating mixers for down-conversion of the received signal. The application of these transceiver elements to Doppler motion detection is reported.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Quantitative expression of the human kallikrein gene 9 (KLK9) in ovarian cancer: a new independent and favorable prognostic marker. (2001)

Many members of the human kallikrein gene family were found to be differentially expressed in various malignancies and some are useful cancer diagnostic/prognostic markers. KLK9 is a newly discovered human kallikrein gene that is expressed in several tissues including thymus, testis, spinal cord, salivary gland, ovary, and skin. Like other kallikreins, the KLK9 gene was found to be regulated by steroid hormones in cancer cell lines. Our purpose is to examine whether quantitative analysis of KLK9 expression has prognostic value in ovarian cancer. We studied the expression of KLK9 by quantitative reverse transcription-PCR in 168 consecutive ovarian tumors of different stages, grades, and histological types, and correlated the expression with clinicopathological parameters, response to chemotherapy, and patients' survival. We found that KLK9 expression was significantly higher in patients with early disease stages (I or II; P = 0.044) and in patients with optimal debulking (P = 0.019). Kaplan-Meier survival curves demonstrated that patients with KLK9-positive tumors have substantially longer progression-free and overall survival (P < 0.001 and P = 0.016, respectively). When the Cox proportional hazard regression analysis was applied to subgroups of patients, KLK9 expression was found to be a significant predictor of progression-free survival in the subgroup of patients with low-grade tumors [hazard ratio (HR), 0.13; P = 0.0015], early stage (HR, 0.099; P = 0.031); and those with optimal debulking (HR, 0.26; P = 0.012). After adjusting for other known prognostic variables, KLK9 retained its independent prognostic value in all of these subgroups of patients. A negative correlation was found between the expression levels of CA125 and KLK9 (rs, 0.350; P = 0.002). Our results indicate that KLK9 is under steroid hormone regulation in ovarian and breast cancer cell lines. Immmunohistochemically, human kallikrein protein (hK9) was localized in the cytoplasm, but not in the nuclei, of the epithelial cells of ovarian cancer tissues. We conclude that KLK9 is a potential new independent favorable prognostic marker for early stage, low-grade, optimally debulked ovarian cancer patients.
Influence of a magnetic seed line on the switching behaviour of submicrometre sized magnetic tunnel junctions (2001)

Magnetic tunnel junctions in the sub-micrometre range have been patterned by electron beam lithography. The hard reference electrode is composed of an artificial antiferromagnetic subsystem while the soft magnetic detection layer is Permalloy (NiFe). The magnetic switching characteristic is studied for various seed layers. For a polycrystalline iron seed line the magnetoresistance switching curve is irregular and not well behaved, while for a nonmagnetic seed layer (Ru) the reproducibility is good and the switching can be related to the shape of the element. We discuss possible coupling mechanisms and show that the magnetization reversal of the soft layer is mainly driven by the large stray fields of moving Néel-type domain walls in the Fe seed layer.