gebhart/patrepeval huggingface dataset:
Independent regulation of H-2K and H-2D gene expression in murine teratocarcinoma somatic cell hybrids. (1980)

Cells of two teratocarcinoma stem cell lines (PCC4 azaguanine [aza] 1 and F9 5-bromodeoxyuridine [BrdU]) were fused with normal mouse spleen cells and mouse thymoma-derived cells (BW 5147), respectively. Hybrid clones were tested for the expression of molecules coded by the H-2K and -2D genes both by absorption analysis of conventional H-2 sera and by indirect antibody-binding radioimmunoassay with monoclonal antibodies. Somatic cell hybrids between PCC4 aza 1 and spleen cells morphologically resemble teratocarcinoma stem cells and do not express H-2 antigens. However, after differentiation in vitro, one of these hybrid clones expresses the H-2K and -2D gene products of both parental cell lines, one close expresses H-2-D- but not H-2K-coded antigenic determinants, and one clone remains H-2 negative. Somatic cell hybrids between F9 BrdU and BW 5147 resemble fibroblasts. Analysis of a series of hybrid clones revealed some clones that express both the H-2K- and H-2D-coded antigenic specificities of both parental alleles, some that express H-2D gene products strongly and the H-2K gene products very weakly, and some that express H-2D- but not H-2K-coded molecules. These results imply independent regulation of expression of the H-2K and -2D genes. The H-2D gene products appear to be preferentially expressed if the hybrid cells are capable of expressing H-2. The results suggest complex regulatory mechanisms that are H-2K and H-2D specific.
Multi-Layer Sub-Array Overlapping (2023)

Contiguous sub-array overlapping is investigated for multilayer sub-arrays. The structures are implemented in multiple layers to realize complex sub-array overlapping with a smaller number of crossovers. It is shown that complex sub-array schemes, which might be impossible to realize in one layer, can be implemented in several layers with less complication. The computed results of array factors and Full-wave simulation results are presented. The Full-wave results are acceptably compatible with design computations.
An intelligent contraflow control method for real-time optimal traffic scheduling using artificial neural network, fuzzy pattern recognition, and optimization (2000)

Contraflow operation is frequently used for reducing traffic congestion near tunnels and bridges where traffic demands from the opposite directions vary periodically. In this work, a generic real-time optimal contraflow control method has been introduced. The introduced method integrates two important functional components: 1) an intelligent system with artificial neural network and fuzzy pattern recognition to accurately estimate the current traffic demands and predict the coming traffic demands, and 2) a mixed-variable, multilevel, constrained optimization to identify the optimal control parameters. Application of the developed method to a case study-dynamic contraflow traffic operation at the George Massey Tunnel in Vancouver, BC, Canada-has significantly reduced traffic delay and congestion.
Characterization of a 64-kd protein phosphorylated during chemotactic activation with IL-8 and fMLP of human polymorphonuclear leukocytes. II. Purification and amino acid analysis of phosphorylated 64-kd protein (1993)

Lung giant cell carcinoma-derived chemotactic protein (LUCT)/IL-8 and fMet-Leu-Phe stimulate phosphorylation of a 64-kd protein (p64) in 32P-labeled human polymorphonuclear leukocytes (PMNs). The p64 was purified from cytosol of human PMNs (1.8 x 10(9) cells) by DEAE-Sepharose CL6B column chromatography, hydroxyapatite HPLC, and reverse-phase HPLC. By hydroxyapatite HPLC, p64s were separated and produced two peaks containing both nonphosphorylated and phosphorylated p64. Amino acid composition of each p64 was determined. Each p64 was directly subjected to amino acid sequencing, but the amino acid residue in the amino-terminal of the proteins could not be detected. From the results of amino acid composition and other characters of p64, it is suggested that p64 is identical to l-plastin, which is a leukocyte-specific protein.
Automated Analysis of Structured Online (1996)

We believe that the domain-specific knowledge of the structural organization of information is central to the human ability to deal with large quantities of data efficiently. A better understanding of the computational nature of this ability may lead to solutions to information retrieval problems of practical significance. The paper outlines a Theory of Document Presentation (DPT) which addresses the problems of how information can be structurally organized in the documents of a given domain and how the standards for such organization emerge. The paper describes FAQ Minder, a document processing system whose implementation was guided by DPT. FAQ Minder processes FAQs, files of Frequently Asked Questions associated with USENET newsgroups [1,2]. The system identifies and tags the logical components of FAQs: network headers, tables of contents, sections, glossaries, questions, answers, and bibliographies.
Epigenomic diversity of colorectal cancer indicated by LINE-1 methylation in a database of 869 tumors (2010)

LINE-1 extreme hypomethylators appear to constitute a previously-unrecognized, distinct subtype of colorectal cancers, which needs to be confirmed by additional studies. Our tumor LINE-1 methylation data indicate enormous epigenomic diversity of individual colorectal cancers.
Strengthening of Hearing Ear Representation Reduces Binaural Sensitivity in Early Single-Sided Deafness (2015)

Single-sided deafness initiates extensive adaptations in the central auditory system, with the consequence that a stronger and a weaker ear representation develops in the auditory brain. Animal studies demonstrated that the effects are substantially stronger if the condition starts early in development. Sequential binaural cochlear implantations with longer interimplant delays demonstrate that the speech comprehension at the weaker ear is substantially compromised. A pronounced loss of the ability to extract and represent binaural localisation cues accompanies this condition, as shown in animal models.
Fatal Air Embolism During Radical Retropubic Prostatectomy (1994)

Air embolism during urological surgery only rarely has been reported. We report a case of fatal air embolism occurring during radical retropubic prostatectomy. This entity is discussed in an attempt to raise the level of awareness of this rare but potentially lethal complication, especially in view of the ever increasing popularity of radical retropubic prostatectomy as the treatment for organ confined carcinoma of the prostate.
The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data (2016)

MIRAGE (Minimum Information Required for A Glycomics Experiment) is an initiative that was created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to produce guidelines for reporting results from the diverse types of experiments and analyses used in structural and functional studies of glycans in the scientific literature. As a sequel to the guidelines for sample preparation (Struwe et al. 2016, Glycobiology, 26:907-910) and mass spectrometry data (Kolarich et al. 2013, Mol. Cell Proteomics, 12:991-995), here we present the first version of guidelines intended to improve the standards for reporting data from glycan microarray analyses. For each of eight areas in the workflow of a glycan microarray experiment, we provide guidelines for the minimal information that should be provided in reporting results. We hope that the MIRAGE glycan microarray guidelines proposed here will gain broad acceptance by the community, and will facilitate interpretation and reproducibility of the glycan microarray results with implications in comparison of data from different laboratories and eventual deposition of glycan microarray data in international databases.
Probing the molecular orientation of chemically polymerized polythiophene-polyrotaxane via solid state NMR (2017)

Polyrotaxanes have attracted significant attention of scientific community and are being explored towing to its electro-optical properties. The orientation and the molecular structure of polyrotaxanes influence its final properties. Thus a, detail understanding of the structure of such compounds at the molecular level will provide an opportunity to further tune and optimize their properties. Here chemically polymerized polythiophene polyrotaxane i.e. Thiophene [2]Rotaxane which is the rotaxane like compound consisting of several macrocycle, was studied in the powdered form. This allowed us to investigate the molecular structure of polyrotaxane and to obtain the detail insights into its structure property relationship via solid state NMR. The as-obtained polymer has also been characterized via various other techniques including UV spectroscopy, solution NMR and so on. We elucidated the structure of the Thiophene [2]Rotaxane monomer remained intact even after polymerization.
Identification of FLCN phosphorylation sites at the G2/M boundary. (2015)

&lt;p&gt;(A) U2OS cells were cultured in the presence (lane 2) or absence (lane 1) of nocodazole overnight and immunoprecipitated FLCN protein was detected by Coomassie Blue staining of SDS-PAGE gels. (B) LC-MS/MS analysis of FLCN peptides. Extracted ion chromatograms for FLCN peptide AHSPAEGASVESSSPGPK with no phosphorylation (a), one phosphorylation (b) and 2 phosphorylation sites (c). Intensity corresponds to the absolute intensity of the highest peak in the chromatogram. No normalization was performed. (C) FLCN deficient UOK257 cells were reconstituted with FLCN WT, FLCN double phosphoinactivating mutant S62/73A or FLCN double phosphomimetic mutant S62/73E. Exogenous WT FLCN and the phosphomutants (S62/73A and S62/73E) are expressed in both the nuclear and the cytoplasmic cellular fractions. (D) FLCN deficient UOK257 cells and the isogenic cell lines reconstituted with either FLCN WT, a tumor-associated mutant (FLCN ?F157) or the phosphomutants (S62/73A and S62/73E) were cultured overnight in the absence or presence of nocodazole. (E) UOK257 FLCN WT cells growing asynchronously (Asyn) or arrested during G2/M with nocodazole were treated with either DMSO or cycloheximide (10 μg/ml). Protein stability was determined by Western blot at various time points over the course of 8 hours (V – UOK257 vector only cells; WT – UOK257 FLCN WT cells). Lysates were resolved in SDS-PAGE and FLCN protein detected with anti-FLCN antibody. Actin serves as a loading control.&lt;/p&gt;
[Treatment of solitary renal cysts in children]. (2001)

The authors formulate therapeutic policy basing on clinical, ultrasound and parenchymal morphological studies in 34 children with solitary renal cysts. Operative intervention is indicated in renal function decline manifesting with enlargement of the cyst and reduction of the cystic-renal coefficient. The kind of the operation is decided by location of the cyst and its characteristics obtained at radiodiagnosis. In slow growth of the cyst and no further decline in its function the patients should be followed up as a risk group.
The effects of sodium tanshinone II ^A sulfonate on activation of NF-#dappa#B on hypertrophic myocardial cells (2004)

To explore the effects of sodium tanshinone II A slfonate on hypertrophy induced by angiotensin II (AngiI in cultured neonatal rat mycarial cells. An in vitro cells culture technique was used. The effects of Solium tanshinone II A sulfonate on expression of uclear transciripton factor #kappa#B(NF-#kappa#B) were studied by immunohistochemstry on angiotensin II-induced hypertrophic myoctes. COmpared with normal cultured myocytes, cardiomyocyte diameter in Ang II group were higher and NF-#kappa#B expression increased significanlty (P<0.01). Sodium tanshinone II A sulfonate signifcantly suppressed angiotensin II-induced hypertrophy of cells and reduced NF-#kappa#B expression (P<0.05). Sodium tanshinone IIA sulfonate may be beneficial in prodtecting the myocardium against hypertrophy. The suppression of myocardial hypertrophy might be associated with the suppression of NF-#kappa#B expression caused by sodium tanshinone IIA sulfonate.
<title>Fluorescence line-narrowing spectroscopy in the study of chemical carcinogenesis</title> (1991)

A laser spectroscopic technique known as 'fluorescence line narrowing spectroscopy' (FLNS) provides an unprecedented level of detail in the study of cellular macromolecular damage to DNA due to chemical reactions in vitro and in vivo with organic carcinogens. The reactions are believed to be the first step in the induction of tumors. Different stereoisomers of the metabolite benzo[a]pyrene diol epoxide (BPDE) which binds to DNA bases and the same stereoisomers in different DNA site configurations can be directly distinguished. By comparing BPDE adducts formed from synthetic polynucleotides of specific base composition, insights into the nature of BPDE-DNA adduct structure types can be obtained. It is shown that FLNS can be used to study metabolic pathways and DNA damage routes from benzo[a]pyrene and 7,12- dimethylbenzanthracene. BPDE-human hemoglobin adducts can also be identified. Due to its high sensitivity and superior selectivity, FLNS could be the basis for a practical and reliable body burden assessment methodology.
Pulse Electroplating of Silver-Tin Alloys and the Formation of Ag[sub 3]Sn (1973)

Silver‐tin alloys were electrodeposited at 50° by pulse plating from a bath containing ; ; ; and . The major variables studied were pulse cycle and current density. In the current density range of 1.5–3 A/dm2 with pulse on‐times of 5–100 msec and off‐times of 50–200 msec, the major constituent in the deposit was the intermetallic compound Ag3Sn. Several experimental conditions were determined in which the elemental tin and silver contents were small. The Ag3Sn coatings showed excellent integrity at thicknesses up to cm and were highly ductile. The tarnish resistance of the coatings was superior to that of pure silver.
A polyphosphoester-conjugated camptothecin prodrug with disulfide linkage for potent reduction-triggered drug delivery (2015)

A new kind of reduction-cleavable polymer-camptothecin (CPT) prodrug has been developed, in which the polymer backbone consists of a biodegradable diblock polyphosphoester (PBYP-b-PEEP), and a modified CPT is linked onto the pendant alkynes of PBYP via Cu(i)-catalyzed azide-alkyne cycloaddition (CuAAC) "click" reaction to yield the polymeric prodrug, abbreviated as (PBYP-g-ss-CPT)-b-PEEP. The resulting prodrug could self-assemble into uniform prodrug micelles in aqueous solution. Since the releasable disulfide carbonate between the CPT and the polyphosphoester would be disrupted under an intracellular reducing environment, the disassociation of prodrug micelles could result in a rapid release of the CPT parent drug. The chemical structures of the intermediate polymers and a polymeric prodrug have been fully characterized by <sup>1</sup>H NMR and FT-IR analyses, while the molecular weights and molecular weight distributions were measured by gel permeation chromatography (GPC). The self-assembly behavior of the prodrug was investigated by the fluorescence probe method, dynamic light scattering (DLS) and transmission electron microscopy (TEM) analyses. The DLS results indicated that these prodrug micelles were relatively stable in neutral pH media, but could be degraded under the reductive conditions. The in vitro drug release studies showed that the CPT release from prodrug micelles was proceeded in a glutathione (GSH)-dependent manner. A methyl thiazolyl tetrazolium (MTT) assay demonstrated that the prodrug micelles could efficiently inhibit the proliferation of HepG2 cells. In addition, the intracellular uptake of prodrug micelles could efficiently release CPT into HepG2 cells, which was observed using a live cell imaging system. All these results indicated that this GSH-responsive polymeric prodrug has high potential for reduction-triggered cancer chemotherapy.
Foundations of Modern Query Languages for Graph Databases (2017)

We survey foundational features underlying modern graph query languages. We first discuss two popular graph data models: edge-labelled graphs , where nodes are connected by directed, labelled edges, and property graphs , where nodes and edges can further have attributes. Next we discuss the two most fundamental graph querying functionalities: graph patterns and navigational expressions . We start with graph patterns, in which a graph-structured query is matched against the data. Thereafter, we discuss navigational expressions, in which patterns can be matched recursively against the graph to navigate paths of arbitrary length; we give an overview of what kinds of expressions have been proposed and how they can be combined with graph patterns. We also discuss several semantics under which queries using the previous features can be evaluated, what effects the selection of features and semantics has on complexity, and offer examples of such features in three modern languages that are used to query graphs: SPARQL, Cypher, and Gremlin. We conclude by discussing the importance of formalisation for graph query languages; a summary of what is known about SPARQL, Cypher, and Gremlin in terms of expressivity and complexity; and an outline of possible future directions for the area.
ChemInform Abstract: Total Syntheses of (+)‐Trienomycins A and F via a Unified Strategy. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Mode of protective action of fosfomycin against dibekacin-induced nephrotoxicity in the dehydrated rats. (1982)

We studied the mechanism on protective effect of fosfomycin against experimental nephrotoxicity induced by dibekacin. In order to simplify an experimental model, the dehydrated Wistar rats were used, because a single injection of dibekacin at 30 mg/kg induced acute renal failure in the dehydrated rats, characterized by alteration of urinalytic parameters and BUN values, and retarded elimination of dibekacin from blood. When the rats were administered simultaneously with fosfomycin at 120 mg/kg, the rate of elimination was restored almost to normal, accompanied with improvement of the nephrotoxic parameters. However, markedly accelerated elimination over normal one was not observed, indicating that the improved elimination was not the reason of protection but a result of normal kidney function. On the other hand, fosfomycin protected the proximal tubular lysosomes from the injury of aminoglycoside, as evidenced a) in vivo by suppression of myeloid body formation and protection of lysosomal membrane integrity of the rats treated with dibekacin, and b) in vitro by dose-dependent protection of the lysosomal membrane integrity of the kidney cells. A study of structure-protective activity relation revealed that phosphonate anion possessing an epoxy function was important for protection, and that the mechanism of protection differed from the antibacterial mechanism.
Use of Data Compression in Gaussian Beam Migration Algorithm (2018)

Summary Petroleum industry is facing a raise of data volume. Especially in seismic where survey areas are increasing and new acquisition techniques are becoming affordable such as WATS or OBS, workarounds has to be implemented to face this increasing amount of data. In this paper we propose an approach to run a Gaussian beam migration algorithm using lossy data compression by taking advantage of the data size reduction and the IO bandwidth saved, without affecting the final output gather quality. This compression algorithm offers enough efficient to even reduce the time dedicated to access the data.
The future risk of primary biliary cholangitis (PBC) is low among patients with incidental anti‐mitochondrial antibodies but without baseline PBC (2022)

Anti-mitochondrial autoantibodies (AMAs) are highly specific for the diagnosis of primary biliary cholangitis (PBC) but are also occasionally found in other diseases. In the present study, we evaluated the incidence of and predictors for PBC development in AMA-positive patients with other liver or non-liver diseases at baseline. In this retrospective study, we screened patients who tested positive for AMA and/or anti-mitochondrial M2 antibody (AMA-M2) at Beijing Friendship Hospital, Capital Medical University, from October 2005 to January 2017. They were categorized by their diagnosis at the baseline as patients with PBC or non-PBC cases. We followed up on the non-PBC cases through telephone interviews and reviewing of medical records to obtain laboratory results and clinical outcomes. In total, 139 patients were AMA-positive but did not fulfill the diagnostic criteria of PBC at baseline, including 51 patients with non-PBC liver diseases and 88 cases with non-liver diseases. The titers of AMA-M2, alkaline phosphatase, gamma-glutamyl transpeptidase, and immunoglobulin M were significantly higher in patients with PBC compared to those with non-PBC liver diseases and non-liver diseases. After a median follow-up of 4.6 (interquartile range: 2.4-7.6) years, 4.3% (6 of 139) developed PBC, with an accumulative 5-year incidence rate of 4.2%. None of the patients with non-PBC liver diseases developed PBC, whereas the 5-year incidence rate of PBC was 7.8% among 88 patients with non-liver diseases. Lower alanine aminotransferase and higher immunoglobulin M were independent predictors for developing PBC. Conclusion: Our results suggest a low risk of developing PBC over time in AMA-positive patients with other liver and non-liver diseases.
Creep and Stress Rupture Behavior of an Advanced Silicon Nitride: Part III, Stress Rupture and the Monkman–Grant Relationship (1994)

The applicability of the Monkman–Grant relationship to predict the stress rupture life of NT154 silicon nitride is examined. The data show that the Monkman–Grant lines relating rupture life to minimum creep rate are stratified with respect to temperature. A modification to the current expression for the Monkman–Grant relation is proposed to accommodate this temperature dependence. A phenomenological approach based on crack growth as the failure mechanism is presented to explain this temperature dependence. The results can be interpreted as suggesting that the stress dependence of the failure process is greater than that for creep.
Co-infections with multiple genotypes of Anaplasma marginale in cattle indicate pathogen diversity (2018)

Anaplasma marginale was prevalent in the Western Cape, KwaZulu-Natal and Mpumalanga and absent in the Northern Cape. Anaplasma centrale was prevalent in the Western Cape and KwaZulu-Natal and absent in the Northern Cape and Eastern Cape. None of the cattle in the study were known to be vaccinated with A. centrale, so finding positive cattle indicates that this organism appears to be naturally circulating in cattle. A diverse population of A. marginale strains are found in South Africa, with some msp1α genotypes widely distributed across the country, and others appearing only once in one province. This diversity should be taken into account in future vaccine development studies.