gebhart/patrepeval huggingface dataset:
Microtubules mediate anti-inflammatory and lung vascular barrier protective effects of atrial natriuretic peptide (2014)

Independently on control of circulating fluid, atrial natriuretic peptide (ANP) exhibits anti-inflammatory effects in the lung. We tested a role of microtubule (MT) cytoskeleton in the ANP-induced protection against endothelial cell (EC) barrier dysfunction and acute lung injury induced by Staphylococcus aureus -derived peptidoglican-G (PepG). ANP attenuated PepG-induced Rho signaling via suppression of PepG-induced MT disassembly and inhibition of MT-associated Rho-specific guanine nucleotide exchange factor GEF-H1. ANP-induced EC barrier preservation and MT stabilization was due to phosphorylation and inactivation of MT-associated protein stathmin. Phosphorylation deficient stathmin-S63A mutant abolished ANP protective effects against PepG-induced hyperpermeability, activation of Rho and NFkB signaling, and expression of inflammatory adhesion molecules. In contrast, siRNA-based stathmin knockdown prevented PepG-induced peripheral MT disassembly and EC barrier dysfunction. ANP protective effects in murine model of PepG-induced lung injury were associated with increased acetylated MT and phospho-stathmin levels, while exacerbated lung injury in the ANP knockout mice was accompanied by decreased pool of stable MT. Stathmin knockdown in vivo reversed exacerbation of lung injury in ANP knockout mice. These results show a novel mechanism of EC barrier protection and mitigation of inflammation by ANP in EC culture and in the in vivo model of lung injury via stathmin-dependent MT assembly and MT-regulated Rho signaling. Funding: HL087823, HL107920.
Design and Simulation of a Control System for a Quadcopter with a Safety Harness (2018)

This paper presents a control scheme and simulation results for the quadrotor with a safety harness to prevent crash physically. We propose the mechanical system consisted of the quadrotor and the 2-link passive joint arm as the safety harness. Physical interaction, however, occurs between the safety harness and the fixed object and unstabilize the quadrotor. In order to solve this problem, we design the control scheme including Integral Backstepping Control and Admittance Filter technique. The simulations have been carried out in four case studies on the virtual robot experimentation platform (V-REP). The simulation results show the possibility that the safety harness on the quadrotor performs as the protective equipment.
Sentinel lymph node detection with the use of SPECT-CT in endometrial cancer--analysis of two cases. (2012)

On the basis of two cases we discuss the important issues regarding the sentinel lymph node detection biopsy (SLNB) in endometrial cancer with combined cervical administration of the radiocolloid and the subserosal blue dye injection. The first patient (endometrioid adenocarcinoma G2, invasion > 50% myometrium) had 4 SLNs detected. Three were both hot and blue (detected on SPECT-CT). The fourth, paraaortic SLN was blue only. None of the lymph nodes contained metatstases. The second patient (endometrioid adenocarcinoma G1, invasion > 50% myometrium) had 4 SLNs detected. Three were blue (but two of them had also very low radioactivity). The fourth SLN was hot only. Blue only node contained macrometastasis. In the past patients underwent cervical amputation. Diverse distribution of each tracer confirms the advantages of the combined tracers administration in SLNB. The radiotracer is the crucial component--uptake was present in 6 of 8 SLNs. Although the blue dye is more a complimentary method, its suberosal injection significantly increases the safety of the SLNB procedure. In the first case we have detected blue only SLN in paraaortic region which otherwise would be missed using the cervical approach only. More importantly in the second case the tracer uptake was very limited due to the previous surgery and the blue dye administration allowed correct SLNs detection (including the metastatic node). Presented clinical cases confirms that the combined cervical and subserosal tracers administration together with preoperative SPECT-CT constitute an optimal SLN detection method and correctly provides information about the regional lymph node status.
Abstract W P156: Arterial Stiffness in Older Adults With and Without Stroke (2015)

Introduction: Silent cerebrovascular infarcts resulting from vascular disease can manifest as a decline in cognitive function. These silent events are also associated with increased risk of clinically overt stroke. Arterial stiffness is a marker that represents atherosclerotic progression and is a predictor of cardiovascular events and mortality. This study examined the relationship between arterial stiffness and cognitive impairment between adults aged 50-80 years old with and without stroke. Hypothesis: We hypothesized that elevated arterial stiffness would be observed in individuals with stroke, and also be associated with increased cognitive impairment across all participants. Methods: Cognition was assessed using the Montreal Cognitive Assessment (MoCA). Arterial stiffness was quantified using carotid-femoral pulse wave velocity (cfPWV, in m/s), calculated as cfPWV=D/Δt, where D was the distance measured between arterial sites and Δt was the pulse transit time. Higher values represent increased stiffness, and values &gt;10 m/s are associated with increased risk for cardiovascular events. Results: Twenty-five participants were assessed: 11 participants 4.7±2.4 years post-stroke and 14 older adults without stroke. The non-stroke group was older (73.1±3.9 vs. 65.2±9.4 years, P=0.009), while the stroke group had lower MoCA scores (21.2±3.2 vs. 24.4±2.8, P=0.01). There were no between-group differences in cfPWV (stroke 9.4 m/s vs. older adults 9.9 m/s, P=0.49), when controlling for age and MoCA scores. In backward regression analysis, age explained 21% of the variance of cfPWV (P=0.03), while MoCA was not a contributor. Conclusions: In conclusion, these results suggest that age is a significant correlate of arterial stiffness, regardless of the presence of stroke or cognitive impairment. Ongoing work will examine whether stroke history also contributes to arterial stiffness when groups are matched for age.
Auf dem Weg zur Forschungsbibliothek (2016)

Michael Knoche, von 1991 bis 2016 Direktor der Herzogin Anna Amalia Bibliothek, Klassik Stiftung Weimar, hat sich in den 25 Jahren seiner Tätigkeit intensiv mit dem Thema Forschungsbibliothek beschäftigt. In diesem Band sind seine Einsichten und Erfahrungen zusammengestellt. Das Buch gliedert sich in drei Teile und einen autobiographischen Epilog. Den Anfang macht ein bibliothekstheoretischer bzw. bibliothekspolitischer Teil. Im zweiten Teil sind es die besonderen Momente der jüngsten Geschichte der Herzogin Anna Amalia Bibliothek (darunter der Brand der Bibliothek im Jahr 2004), die Anlass für die Frage gaben, wie unter den gegebenen Bedingungen das Forschungsbibliothekskonzept verwirklicht werden kann. Im dritten Teil werden Persönlichkeiten vorgestellt, die - sei es als Wissenschaftler, Strategen, Kooperationspartner, Geldgeber oder Kollegen - eine wichtige Rolle im Netzwerk der Bibliothek gespielt haben.
Advanced materials and processes for addressing renewable energy challenges (2018)

This paper highlights the problems associated with forecasted energy demand growth and how they could be met with new energy generation and technologies. Even though, the concepts of renewable energy conversion and storage are known, our ability to implement them on a global scale is limited due to several challenges with scalable manufacturing, efficiency and durability. The challenges with current technologies can be addressed through discovery and development of advanced materials and processes.
A Rapid Method for the Pre‐Enrichment and Detection of Salmonella Typhimurium by Immunomagnetic Separation and Subsequent Fluorescence Microscopical Techniques (2005)

Abstract Detection of food‐borne pathogens is of great importance in order to minimize the risk of infection for customers. These analyses should be as fast as possible. Any detection method requires enrichment and quantitative analysis of the enriched microbes. Conventional enrichment methods, which take several days, need to be replaced by faster techniques such as immunomagnetic separation (IMS). This technique is based on the use of paramagnetic microspheres coated with antibodies as ligands that have specific affinity to the microbes that have to be detected. In the studies reported here, a rapid method for the detection of Salmonella enterica serovar Typhimurium ( Salmonella Typhimurium), combining IMS and Direct Epifluorescence Filter Technique (DEFT), was developed. It was focused on releasing the target cells from the magnetic beads after IMS, because this is a premise for combining IMS, as an alternative pre‐enrichment, with DEFT. Otherwise, the high number of beads form a layer on the filter membrane that makes the following microscopic analysis for the detection of the contaminants impossible. The CELLection TM Dynabeads ® used in this study, are coated with recombinant streptavidin (rSA) via a DNA linker. The rSA binds biotinylated antibodies that are able to capture target cells. The DNA linker provides the cleavable site, so that the beads can be removed from the captured cells after isolation. In this study a releasing procedure was developed. This procedure allows for an average 74 % ± 4 % of the bead‐bound Salmonella Typhimurium cells to be released from the beads after IMS, so that the detection of the separated cells by DEFT will be possible.
Crystal structures of [W(CO)5(PPh3)], [M(CO)5(AsPh3)] and [M(CO)5(SbPh3)](M = Mo or W): a comparative study of structure and bonding in [M(CO)5(EPh3)] complexes (E = P, as or Sb; M = Cr, Mo or W) (1994)

Crystal structures have been determined for [W(CO)5(PPh3)], [M(CO)5(AsPh3)] and [M(CO)5-(SbPh3)](M = Mo or W) as part of a systematic study of a series of nine [M(CO)5(EPh3)] complexes (M = Cr, Mo or W; E = P, As or Sb). Trends in bond lengths and angles are rationalised in terms of steric and electronic interactions between the bonded M(CO)5 and EPh3 fragments. Comparison is made between the solid-state geometry of the free and co-ordinated EPh3. Torsion angles defining the disposition of the EPh3 groups in relation to M(CO)5 show little variation within the [M(CO)5(EPh3)] series. For all nine complexes the EPh3 groups have propeller geometry with small deviations from symmetric C3 structures. The studies were extended to include other related [M(CO)5L] complexes. Trends in the structural parameters of 29 such compounds are related to the steric behaviour and π-acceptor capacity of the ligands L. It is shown that M–P bond dimensions are not adequately explained by hybridisation changes of the phosphorus atom in the ligand L.
Yeast iso‐l‐cytochrome c: Genetic analysis of structural requirements (1988)

We describe the use of classical and molecular genetic techniques to investigate the folding, stability, and enzymatic requirements of iso-1-cytochrome c from the yeast Saccharomyces cerevisiae. Interpretation of the defects associated with an extensive series of altered forms of iso-1-cytochrome c was facilitated by the recently resolved three dimensional structure of iso-1-cytochrome c [(1987) J. Mol. Biol. 199, 295-314], and by comparison with the phylogenetic series of eukaryotic cytochromes c. Residue replacements that abolish iso-1-cytochrome c function appear to do so by affecting either heme attachment or protein stability; no replacements that abolish electron transfer function without affecting protein structure were uncovered. Most nonfunctional forms retained at least partial covalent attachment to the heme moiety; heme attachment was abolished only by replacements of Cys19 and Cys22, which are required for thioether linkage, and His23, a heme ligand. Replacements were uncovered that retain function at varying levels, including replacements at evolutionarily conserved positions, some of which were structurally and functionally indistinguishable from wild type iso-1-cytochrome c.
The Critical Role of Concentration for Lidocaine Block of Peripheral Nerve In Vivo (2003)

Blocks of greater depth and longer duration result from injection of smaller volumes and, correspondingly, higher lidocaine concentrations containing the same dose. The corollary is that lower lidocaine doses are required to achieve the same effect when smaller volumes are injected. Curiously, when the equivalent E50 is injected, total drug taken into the nerve is less from the smaller volumes than from the larger volumes, even though the peak functional effects are equal. Total intraneural local anesthetic may not represent the effective drug in the compartment that contains nerve axons, the actual location of neural blockade.
Birkhoff-von Neumann Graphs that are PM-compact (2018)

A well-studied geometric object in combinatorial optimization is the perfect matching polytope of a graph $G$. In any investigation concerning the perfect matching polytope, one may assume that $G$ is matching covered --- that is, it is a connected graph (of order at least two) and each edge lies in some perfect matching. A graph $G$ is Birkhoff-von Neumann (BvN) if its perfect matching polytope is characterized solely by non-negativity and degree constraints. A result of Balas (1981) implies that $G$ is BvN if and only if $G$ does not contain a pair of vertex-disjoint odd cycles $(C_1,C_2)$ such that $G-V(C_1)-V(C_2)$ has a perfect matching. It follows immediately that the corresponding decision problem is in co-NP. However, it is not known to be in NP. The problem is in P if the input graph is planar --- due to a result of Carvalho, Lucchesi and Murty (2004). These authors, along with Kothari (2018), have shown that this problem is equivalent to the seemingly unrelated problem of deciding whether a given graph is $\overline{C_6}$-free. The combinatorial diameter of a polytope is the diameter of its $1$-skeleton graph. A graph $G$ is PM-compact (PMc) if the combinatorial diameter of its perfect matching polytope equals one. A result of Chvátal (1975) implies that $G$ is PMc if and only if $G$ does not contain a pair of vertex-disjoint even cycles $(C_1,C_2)$ such that $G-V(C_1)-V(C_2)$ has a perfect matching. Once again the corresponding decision problem is in co-NP, but it is not known to be in NP. The problem is in P if the input graph is bipartite or is near-bipartite --- due to a result of Wang, Lin, Carvalho, Lucchesi, Sanjith and Little (2013). In this paper, we consider the "intersection" of the aforementioned problems. We give a complete characterization of matching covered graphs that are BvN as well as PMc. (Thus the corresponding decision problem is in P.)
Irreverisble differential block of A‐ and C‐fibres following local nerve heating in the cat. (1980)

1. Local heating of a nerve was used to block conduction in the myelinated or A-fibres while preserving normal function in most of the non-myelinated or C-fibres. Compound action potentials of A- and C-fibres, and impulses in single C-fibres were recorded to measure the block. 2. Most experiments were done on the posterior tibial nerve of the cat's hind limb. Conducted heat was applied in successive periods of about 2 min each to 15 mm of the exposed nerve. 3. The range of temperatures used was between 45 and 52 degrees C. Below 46 degrees C no conduction block was obtained. With increasing temperature the cumulative time of heating required to produce a block in at least 99% of A-fibres decreased from around 110 min at 46.5 degrees C to 10 min at 51 degrees C. 4. In the posterior tibial nerve the C-fibre compound action potential was reduced to 0.66 of control (mean of twenty-two experiments) when the A-fibre action potential was less than 1% of control. The corresponding value of this selectivity coefficient was 0.20 in seven superficial peroneal and sural nerves. 5. Once a conduction block of A-fibres was reached the remaining C-fibres showed normal functioning, including conduction of repetitive impulses at up to 30 Hz evoked by electrical nerve and adequate skin stimulation. C-fibre conduction persisted for at least 33 h, but not longer than 3 days. 6. This pure C-fibre nerve might be useful for studying central nervous system effects of C-fibres.
Digital Background Calibration of Capacitor-Mismatch Errors in Pipelined ADCs (2006)

A digital background calibration technique is proposed to correct for the linearity error due to capacitor mismatches in pipelined analog-to-digital converters (ADCs). During the normal ADC operation, it randomly swaps the feedback capacitor with the sampling capacitor(s) in the multiplying digital-to-analog converter (MDAC) of each pipeline stage in the pipelined ADC. The capacitor-mismatch errors in all pipeline stages are then concurrently measured and corrected in the digital domain. The proposed technique can be utilized in both single-bit and multibit MDACs. Owing to its simple iterative algorithm for capacitor-mismatch error calibration, its implementation requires minimal additional digital hardware. Behavioral simulation results show that, using the proposed calibration technique, the signal-to-noise-plus-distortion ratio is improved from 10 to 12.5bits and the spurious-free dynamic range is increased from 65 to 95 dB, in a 13-bit pipelined ADC with sigma=0.25% capacitor mismatches
A Novel Synthesis for Glyoxylic Acid with O_2 Catalysed by Nitrogen Oxide (2005)

A novel method was reported for the synthesis of glyoxylic acid from glyoxal oxidized by O_2 under the catalysis of NO_n (n=1,2). A possible reaction mechanism was proposed. Under the optimized reaction conditions (catalyst 0.13～0.15 mol(per mole glyoxal), mass fraction of glyoxylic acid 25%, Lewis acid 0.11～0.13 mol(per mole glyoxal), 40～50 ℃, 0.5 MPa), a good result was achieved with the conversion 98.7%, selectivity 79% and yield 78%. Compared with the industrial method using nitric acid, the present contribution is more safe, economical and less contaminating the environment.
Are Sutureless and Rapid-Deployment Aortic Valves a Serious Alternative to TA-TAVI? A Matched-Pairs Analysis (2021)

Since sutureless and rapid-deployment aortic valve implantation is as safe and effective as transapical transcatheter aortic valve implantation, combining the advantage of standard diseased-valve removal with shorter procedural times, sutureless and rapid-deployment aortic valve replacement may be considered as an alternative for patients with elevated operative risk considered to be in the "gray zone" between transcatheter aortic valve implantation and conventional surgery, especially if concomitant myocardial revascularization is required.
Yeast‐Based Microporous Carbon Materials for Carbon Dioxide Capture (2012)

A hierarchical microporous carbon material with a Brunauer-Emmett-Teller surface area of 1348 m(2) g(-1) and a pore volume of 0.67 cm(3) g(-1) was prepared from yeast through chemical activation with potassium hydroxide. This type of material contains large numbers of nitrogen-containing groups (nitrogen content >5.3 wt%), and, consequently, basic sites. As a result, this material shows a faster adsorption rate and a higher adsorption capacity of CO(2) than the material obtained by directly carbonizing yeast under the same conditions. The difference is more pronounced in the presence of N(2) or H(2)O, showing that chemical activation of discarded yeast with potassium hydroxide could afford high-performance microporous carbon materials for the capture of CO(2).
Shift of the reaction equilibrium at high pressure in the continuous synthesis of neuraminic acid (2022)

The importance of a compound that helps fight against influenza is, in times of a pandemic, self-evident. In order to produce these compounds in vast quantities, many researchers consider continuous flow reactors in chemical industry as next stepping stone for large scale production. For these reasons, the synthesis of <i>N</i>-acetylneuraminic acid (Neu5Ac) in a continuous fixed-bed reactor by an immobilized epimerase and aldolase was investigated in detail. The immobilized enzymes showed high stability, with half-life times > 173 days under storage conditions (6 °C in buffer) and reusability over 50 recycling steps, and were characterized regarding the reaction kinetics (initial rate) and scalability (different lab scales) in a batch reactor. The reaction kinetics were studied in a continuous flow reactor. A high-pressure circular reactor (up to 130 MPa) was applied for the investigation of changes in the position of the reaction equilibrium. By this, equilibrium conversion, selectivity, and yield were increased from 57.9% to 63.9%, 81.9% to 84.7%, and 47.5% to 54.1%, respectively. This indicates a reduction in molar volume from <i>N</i>-acetyl-ᴅ-glucosamine (GlcNAc) and pyruvate (Pyr) to Neu5Ac. In particular, the circular reactor showed great potential to study reactions at high pressure while allowing for easy sampling. Additionally, an increase in affinity of pyruvate towards both tested enzymes was observed when high pressure was applied, as evidenced by a decrease of <i>K</i> <sub>I</sub> for the epimerase and <i>K</i> <sub>M</sub> for the aldolase from 108 to 42 mM and 91 to 37 mM, respectively.
Splicing is required for rapid and efficient mRNA export in metazoans (1999)

Pre-mRNA splicing is among the last known nuclear events before export of mature mRNA to the cytoplasm. At present, it is not known whether splicing and mRNA export are biochemically coupled processes. In this study, we have injected pre-mRNAs containing a single intron or the same mRNAs lacking an intron (Deltai-mRNAs) into Xenopus oocyte nuclei. We find that the spliced mRNAs are exported much more rapidly and efficiently than the identical Deltai-mRNAs. Moreover, competition studies using excess Deltai-mRNA indicate that different factor(s) are involved in the inefficient export of Deltai-mRNA vs. the efficient export of spliced mRNA. Consistent with this conclusion, spliced mRNA and Deltai-mRNA, though identical in sequence, are assembled into different messenger ribonucleoprotein particles (mRNP) in vitro. Strikingly, the mRNA in the spliced mRNP, but not in the Deltai-mRNP, is exported rapidly and efficiently. We conclude that splicing generates a specific nucleoprotein complex that targets mRNA for export. Our results, revealing a link between splicing and efficient mRNA export, may explain the reports that an intron is required for efficient expression of many protein-coding genes in metazoans.
ChemInform Abstract: THE SYNTHESIS OF 8‐OXOBERBINES CONTAINING A HETEROCYCLIC D‐RING BY ENAMIDE PHOTOCYCLIZATIONS (1979)

Abstract Bei der Behandlung mit entsprechenden Säurechloriden erhält man aus dem 1‐Methyl‐3,4‐dihydro‐isochinolin die 1‐Methylen‐Derivate (IIa) und (IIb), deren Photolyse über diskutierte Zwischenstufen zu den Kondensaten (III) führt.
One-Pot o-Nitrobenzenesulfonylhydrazide (NBSH) Formation−Diimide Alkene Reduction Protocol (2009)

A one-pot protocol for the formation of 2-nitrobenzenesulfonylhydrazide (NBSH) from commercial reagents and subsequent alkene reduction is presented. The transformation is operationally simple and generally efficient for effecting diimide alkene reductions. A range of 16 substrates have been reduced, highlighting the unique chemoselectivity of diimide as a reduction system.