gebhart/patrepeval huggingface dataset:
The CXC Chemokine-degrading Protease SpyCep of Streptococcus pyogenes Promotes Its Uptake into Endothelial Cells (2010)

Streptococcus pyogenes expresses the LPXTG motif-containing cell envelope serine protease SpyCep (also called ScpC, PrtS) that degrades and inactivates the major chemoattractant interleukin 8 (IL-8), thereby impairing host neutrophil recruitment. In this study, we identified a novel function of SpyCep: the ability to mediate uptake into primary human endothelial cells. SpyCep triggered its uptake into endothelial cells but not into human epithelial cells originating from pharynx or lung, indicating an endothelial cell-specific uptake mechanism. SpyCep mediated cellular invasion by an endosomal/lysosomal pathway distinct from the caveolae-mediated invasion pathway of S. pyogenes. Recombinant expression and purification of proteolytically active SpyCep and a series of subfragments allowed functional dissection of the domains responsible for endothelial cell invasion and IL-8 degradation. The N-terminal PR domain was sufficient to mediate endothelial cell invasion, whereas for IL-8-degrading activity, the protease domain and the flanking A domain were required. A polyclonal rabbit serum raised against the recombinant protease efficiently blocked the invasion-mediating activity of SpyCep but not its proteolytic function, further indicating that SpyCep-mediated internalization is independent from its enzymatic activity. SpyCep may thus specifically mediate its own uptake as secreted protein into human endothelial cells.
Accounting for pairwise distance restraints in FFT-based protein–protein docking (2016)

The software is freely available as part of the ClusPro web-based server at http://cluspro.org/nousername.php CONTACT: midas@laufercenter.org or vajda@bu.eduSupplementary information: Supplementary data are available at Bioinformatics online.
Methylsulfonyl Metabolites of PCBs and DDE in Human Milk in Sweden, 1972-1992 (1996)

A multicomponent method used for analysis ob iphenyls (PCBs), napht , dib analysis. of y nIo of d (M `O2-DDE in human Mlk T t partitining. Adtonal p-O and permeation chromography. Te mean reoeri dards, added to the milk before aion, were dur 1972
Vision-Based Drone Flocking in Outdoor Environments (2021)

Decentralized deployment of drone swarms usually relies on inter-agent communication or visual markers that are mounted on the vehicles to simplify their mutual detection. This letter proposes a vision-based detection and tracking algorithm that enables groups of drones to navigate without communication or visual markers. We employ a convolutional neural network to detect and localize nearby agents onboard the quadcopters in real-time. Rather than manually labeling a dataset, we automatically annotate images to train the neural network using background subtraction by systematically flying a quadcopter in front of a static camera. We use a multi-agent state tracker to estimate the relative positions and velocities of nearby agents, which are subsequently fed to a flocking algorithm for high-level control. The drones are equipped with multiple cameras to provide omnidirectional visual inputs. The camera setup ensures the safety of the flock by avoiding blind spots regardless of the agent configuration. We evaluate the approach with a group of three real quadcopters that are controlled using the proposed vision-based flocking algorithm. The results show that the drones can safely navigate in an outdoor environment despite substantial background clutter and difficult lighting conditions. The source code, image dataset, and trained detection model are available at https://github.com/lis-epfl/vswarm.
A practical methodology for experimental fault injection to test complex network-based systems (2009)

A methodology for dependability assessment of complex computer systems, such as fault tolerant grids, is presented in this paper. The methodology uses communication fault injection and was built by adapting a widely accepted approach for performance analysis. To demonstrate its applicability and usefulness, the methodology was applied to a third party grid platform using a fault injector we are developing. The paper reasons about the advantages of this methodology to perform fault injection campaigns in the prototype phase of a system.
FAMC bitstream description (2007)

ISO/IEC JTC1/SC29/WG11, MPEG2007/M14491 , San Jose, CA
THE EFFECT OF RENAL ISCHEMIA ON CYCLOSPORINE CLEARANCE IN RABBITS (1990)

The renal handling of cyclosporine was studied in ischemically damaged kidneys in New Zealand White rabbits and nonischemic control animals. CsA, 25 mg/kg/day, was administered intravenously for 10 days starting with the day of operation. Blood CsA (B CsA) was higher in the ischemic group compared with the controls (median: 285 micrograms/L, range 95-785 micrograms/L vs. 170 micrograms/L, range 110-185 micrograms/L, P = 0.05) on day 1 after operation. B CsA dropped rapidly to a level equivalent to the controls by day 4 (median: 105 micrograms/L, range 60-280 micrograms/L vs. 195 micrograms/L, range 70-215 micrograms/L, P = NS). Median CsA clearance (C CsA) as a percentage of creatinine clearance (C Cr) was some ten-fold greater in the ischemic animals (6.32%, range 2.93-18.41% vs. 0.55%, range 0.13-0.78%, P less than 0.001) on day 1. The ratio gradually declined, approaching the value in controls by day 10 (0.86%, range 0.24-7.21% vs. 0.23%, range 0.16-0.73%, P = 0.05). The data suggest that renal impairment has an important effect on CsA blood levels. In the clinical situation this may be of particular importance during both oliguria and the recovery from acute tubular necrosis.
Metabolic Syndrome in Adolescents (2014)

This issue of AM:STARs, Hot Topics in Adolescent Health, presents a wide array of articles exploring some of the most exciting advances and controversies in adolescent health. These topics and other evolving areas are presented to guide the reader toward providing state of the art clinical care to adolescents, as well as reviewing new research that will shape the future of adolescent health.Topics include:Nutritional and metabolic controversies including the diagnosis of gluten intolerance, vitamin D deficiency and metabolic syndrome in adolescents, and the use of bariatric surgery to treat the comorbidities of adolescent obesity.New diagnostic considerations, including updated DSM-5 diagnostic criteria for mental health disorders such as mood dysregulation, eating disorders, and ADHD.Reproductive health advances including new diagnostic techniques and treatment regimens for HIV and other sexually transmitted infections, as well as the expanding use of long-acting reversible contraceptives.New frontiers in adolescent medicine including office-based management of opiate addiction, support of gender nonconforming youth, and the use of mindfulness practices in the care of a variety of conditions.AM:STARs: Adolescent Medicine: State of the Art Reviews is the official publication of the American Academy of Pediatrics Section on Adolescent Health. Published 3 times per year, the journal offers adolescent medicine specialists and other primary care physicians who treat adolescent patients with state of the art information on all matters relating to adolescent health and wellness.
DSS MOSFET With Tunable SDE Regions by Fluorine Pre-Silicidation Ion Implant (2010)

Fluorine pre-silicidation ion implant (F-PSII) is shown to enable reduction of the lateral source/drain extension junction depth (Xj,SDE) in NiSi dopant-segregated Schottky (DSS) source/drain SOI MOSFETs formed by implant-to-silicide (ITS) with P. F segregation at the NiSi/Si interface reduces the amount of Ni rejection from the NiSi during post-ITS anneal, which reduces the spatial vacancy distribution near the NiSi/Si interface, in turn reducing Xj,SDE and therefore short channel effects. The leakage floor l <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">min</sub> and electron Schottky barrier height are also reduced with F-PSII. This finding reveals new opportunities for single silicide DSS CMOS, whereby DSS MOSFETs can be individually tuned with F-PSII dose to obtain the optimal X <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">j,</sub> SDE.
Solid Solubility of Carbon in Copper during Mechanical Alloying (1998)

Solubility of carbon in solid copper during mechanical alloying using an Attritor type of ball mill was investigated by means of X-ray diffraction, microscopy, electron probe microanalysis and chemical analysis. Carbon atoms less than about 28.5 atomic percent are soluble in the solid copper consisting of nano-crystals during mechanical alloying of copper-graphite mixed powders. The supersaturated solute carbon atoms take the interstitial positions in the fcc α-Cu solid solution and result in the lattice expansion of the α-Cu.
Evaluation of renaturation methods for industrial obtaining of recombinant proteins from Escherichia coli inclusion bodies in biologically active form (2004)

Одной из широко применяемых стратегий для получения рекомбинантных белков в клетках Е. coli является их накопление при экспрессии в виде нерастворимых цитоплазматических агрега­тов — телец включения. Преимущество такого синтеза обусловлено высокими уровнями накоп­ления и чистоты целевого белка, а также возможностью проведения успешной ренатурации и получения активного продукта из нерастворимых агрегатов в условиях in vitro. Основная проблема, препятствующая индустриализации этого процесса, обусловлена отсутствием универ­сальных схем ренатурации, так как рефолдинг рекомбинантных белков является эмпирическим процессом и требует разработки уникальных протоколов для индивидуальных белков. Изучение основных путей агрегации, а также процессов фолдинга in vivo позволило разработать эффектив­ные подходы для рефолдинга многих рекомбинантных белков. В обзоре дана оценка некоторым современным методам ренатурации белков и описаны основные стратегии, которые могут быть использованы для промышленного получения белков из бактериальных телец включения. Кратко рассмотрены недавние достижения в области ренатурации рекомбинантных белков, содержащих дисульфидные связи (окислительный рефолдинг), а также причины, вызывающие агрегацию белков в процессе ренатурации in vitro, и подходы, применяемые для увеличения выхода правильно ренатурированного продукта.
Culture of prostatic epithelial cells from ultrasound‐guided needle biopsies (1991)

A protocol which was developed for the culture of epithelial cells from radical prostatectomy specimens was slightly modified to permit the culture of cells from ultrasound-guided prostatic needle biopsies. The collagenase digestion step of the standard protocol was omitted, and biopsies were simply minced and allowed to attach to collagen-coated dishes in serum-free medium. Cell outgrowths from biopsies were free of fibroblasts, and expression of keratin, prostate specific antigen, and prostatic acid phosphatase was maintained in vitro. The establishment of a bank of frozen cells from primary cultures permitted repetitive studies with individual cell strains, which could be serially passaged and were capable of clonal growth. The ability to derive cultures from biopsies will facilitate the biological characterization of cells from primary prostate tumors of high malignant grade, which are not commonly available from radical prostatectomy specimens.
Strategies for realizing optical CDMA for dense, high-speed, long span, optical network applications (2000)

Since the mid 1990s, the role of optical CDMA has expanded from local area networks to longer span, telecommunication-type networks. In order to play a significant role in these longer span, denser, higher data rate networks, optical CDMA code set must (1) have at least as many codes as dense wavelength division multiplexing (WDM) (i.e., more than eight codes); (2) operate at high data rates (i.e., greater than 2.5 Gb/s); and (3) propagate with high fidelity over the installed or installable fiber links. Most approaches to optical CDMA require narrow pulses, which are more susceptible to fiber impairments and may have lower spectral efficiency than conventional WDM modulation schemes such as non-return-to-zero (NRZ), so they do not meet these new requirements. Therefore, we have formulated a strategy which simultaneously increases the number of good codes (resulting in higher density) and reduces their code length (i.e., decreasing the number of time slots required thus enabling higher data rates for a given chip time): the strategy of matrix codes. In this paper, we describe the design of a set of eight matrix codes for operation at 2.5 Gb/s and evaluate their propagation over an existing 214 km network link by means of computer simulation. The results indicate that the codes propagate well if dispersion management is used. The paper also discusses a strategy for managing the multiaccess interference (MAI) in a bursty traffic environment.
Lidocaine release from polycaprolactone threads (2010)

Abstract In the field of resorbable devices, drug eluting sutures represent an applied research field on which the reliability of both production processes and mathematical prediction modeling were tested. Indeed, poly‐ε‐caprolactone pellets were compounded with lidocaine and then extruded to obtain highly loaded threads. The complete and rapid release demonstrated that the extrusion process does not alter the drug, which is confirmed to be embedded in an open porous matrix, being free to be solvated by uptaken water and to diffuse. Release profile and polymer degradation were simulated through a mathematical model based on conservation laws that allowed to assess release kinetic and to confirm the understanding of involved phenomena, as it fit experimental data. Reliability and robustness of chosen model allow to monitor the overall quality of manufacturing because any discrepancy between experimental and simulated data can be adopted to assess drug distribution uniformity within the device. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Market structure, firm behavior and performance - subsector evidence (1988)

In the 1980s Pakistan's manufacturing sector resumed the rapid growth that had characterized the post-independence period of the 1950s and 1960s. This renewal has coincided with a policy of deregulating investment and price controls, beginning in the late 70s. This report supports the reasoning behind the Government's deregulation policy. It analyzes the impact of key regulations on market structure, firm behavior, and industry performance in key subsectors, focusing on investment sanctioning, price controls and policies affecting firm restructuring and closure (i.e. banking and labor regulation). It concludes that these regulations have in the past been at the. root of poor performance and inefficiencies in the industrial sector, but that the deregulation policy is moving to correct these causes and should stimulate improved industrial performance if it is continued. The main objectives of the report are to examine the impact that previous regulatory policies have had on market structure, firm behavior and industry performance, and to evaluate the preliminary supply and competitive responses to recent relaxations in regulatory policies. The study also highlights potential issues of supply response that might arise in the context of further trade policy reforms, and outlines issues of timing and coordination between trade and regulatory policy reforms. The emphasis is on the private sector since it is expected to be the principal source of new industrial investment, although the effects of public ownership on market structure, behavior and performance are reviewed for some subsectors.
The prevalence of sacroiliac joint CT and MRI findings is high in large breed dogs (2022)

Sacroiliac joint (SIJ) disease has been described as one of the possible causes of lumbosacral (LS) region pain in dogs. However, published information is currently lacking for the computed tomographic (CT) and magnetic resonance imaging (MRI) characteristics of canine SIJ disease. The objectives of this retrospective, observational study were to describe and quantify CT and MRI SIJ findings in a sample of large breed dogs and test associations between the numbers of SIJ findings and other variables. Data archives for a veterinary teaching hospital were searched for large breed dogs (≥ 22.7 kg) that had CT or MRI scans of the LS and pelvic regions in 2015-2019. Dogs with a history of acute trauma or scans with incomplete SIJs were excluded. A veterinary student recorded medical record findings. A veterinary radiologist and graduate student recorded CT and MRI findings based on previously published criteria in dogs and humans. Fifty-three dogs were sampled (20 CT, 33 MRI). Categories of findings with the highest prevalence were subchondral erosion (100% CT, 100% MRI) and subchondral sclerosis (95% CT, 97% MRI). The total numbers of SIJ findings per dog were not associated with dog age, sex, weight, or concurrent findings in the LS or pelvic regions. The total number of MRI SIJ findings per dog differed between German Shepherds and Labrador Retrievers (P = 0.0237) as well as between Labrador Retrievers and other breeds (P = 0.0414). These results indicated that CT and MRI findings reported in humans with SIJ disease are common in large breed dogs.
Orthopaedic interventions in patients with psoriatic arthritis: a descriptive report from the SPAR cohort (2016)

The number of surgical interventions has significantly increased in patients with PsA compared with historical cohorts even with a relatively shorter disease duration. There was a significant difference in HAQ score between the patients with or without surgeries.
Synaptic transmission in nucleus tractus solitarius is depressed by Group II and III but not Group I presynaptic metabotropic glutamate receptors in rats (2002)

Presynaptic metabotropic glutamate receptors (mGluRs) serve as autoreceptors throughout the CNS to inhibit glutamate release and depress glutamatergic transmission. Both presynaptic and postsynaptic mGluRs have been implicated in shaping autonomic signal transmission in the nucleus tractus solitarius (NTS). We sought to test the hypothesis that activation of presynaptic mGluRs depresses neurotransmission between primary autonomic afferent fibres and second-order NTS neurones. In second-order NTS neurones, excitatory postsynaptic currents (EPSCs) synaptically evoked by stimulation of primary sensory afferent fibres in the tractus solitarius (ts) and currents postsynaptically evoked by alpha-amino-3-hydroxy-4-isoxazoleproprionic acid (AMPA) were studied in the presence and absence of mGluR agonists and antagonists. Real-time quantitative RT-PCR (reverse transcription-polymerase chain reaction) was used to determine whether the genes for the mGluR subtypes were expressed in the cell bodies of the primary autonomic afferent fibres. Agonist activation of Group II and III but not Group I mGluRs reduced the peak amplitude of synaptically (ts) evoked EPSCs in a concentration-dependent manner while having no effect on postsynaptically (AMPA) evoked currents recorded in the same neurones. At the highest concentrations, the Group II agonist, (2S,3S,4S)-CCG/(2S,1'S,2'S)-2-carboxycyclopropyl (L-CCG-I), decreased the amplitude of the ts-evoked EPSCs by 39 % with an EC50 of 21 microM, and the Group III agonist, L(+)-2-amino-4-phosphonobutyric acid (L-AP4), decreased the evoked EPSCs by 71 % with an EC50 of 1 mM. mRNA for all eight mGluR subtypes was detected in the autonomic afferent fibre cell bodies in the nodose and jugular ganglia. Group II and III antagonists ((2S,3S,4S)-2-methyl-2-(carboxycyclopropyl)glycine (MCCG) and (RS)-alpha-methylserine-O-phosphate (MSOP)), at concentrations that blocked the respective agonist-induced synaptic depression, attenuated the frequency-dependent synaptic depression associated with increasing frequencies of ts stimulation by 13-34 % and 13-19 %, respectively (P < 0.05, for each). We conclude that Group II and III mGluRs (synthesized in the cell bodies of the primary autonomic afferent fibres and transported to the central terminals in the NTS) contribute to the depression of autonomic signal transmission by attenuating presynaptic release of glutamate.
User Cooperation for Energy-Efficient Cellular Communications (2010)

User cooperation improves performance in wireless systems, but it requires other users to expend energy acting as relays. When energy is scarce, users have incentive to refuse to cooperate in order to conserve resources. Therefore, we investigate cooperative communications from an energy- efficiency perspective. We present a cellular framework in which two mobile users, who desire to communicate with a common base station, may cooperate via decode-and-forward relaying. We maximize users' bits-per-energy efficiency by defining the achievable bits-per-energy region and finding the power allocations that achieve its Pareto boundary. To find an efficient approach that gives selfish users incentive to cooperate, we apply game theory, finding the power allocations that achieve the Nash bargaining solution. Numerical results indicate that the Nash bargain provides a fair and efficient compromise and that both users obtain noticeably improved bits-per-energy efficiency via cooperation.
Hydrogeochemistry of the Northern Barbados Accretionary Complex Transect: Ocean Drilling Project Leg 110 (1990)

Detailed studies of the major element geochemistry, oxygen and hydrogen isotope ratios of pore fluids, and the 87 Sr/ 86 Sr ratio of dissolved strontium have made it possible to unravel physical and chemical processes that affect the pore fluid chemistry in a transect of drill holes across the northern Barbados accretionary complex. These processes include (1) alteration of volcanic ash buried in the Pleistocene‐Pliocene sediment column; (2) alteration of underlying basalts of layer 2 of the oceanic crust; (3) movement of fluids from deep in the accretionary complex along fault zones (particularly the décollement) and minor permeable layers; these fluids from deeper in the complex are characterized by low chloride concentrations and increased δ 18 O(H 2 O) values, presumably as a result of dehydration of smectite interlayers; and (4) mixing processes involving the migrating fluids cause incongruities in the geochemical anomalies of these fluids.
Urinary calculi in Lewis and Wistar rats. (1984)

A high incidence (8/892) of urinary tract calculi was encountered during a study involving rats being fed diets supplemented with retinoids. When the incidence was compared to that observed in earlier studies without retinoid supplements and spanning several years (2/2998), the incidence in the current study was significantly higher. Possible causative factors such as age, sex, strain, diet and carcinogen or retinoid treatment have been analyzed without a clear explanation for the increased incidence. The data suggest that retinoid treatment increased the risk of stone formation, but that retinoids were less important than some other unidentified factor(s) that operated during the recent period.
Ohm's law for mean magnetic fields (1986)

The magnetic fields associated with plasmas frequently exhibit small-amplitude MHD fluctuations. It is useful to have equations for the magnetic field averaged over these fluctuations, the so-called mean field equations. Under very general assumptions, it is shown that the effect of MHD fluctuations on a force-free plasma can be represented by one parameter in Ohm's law, which is effectively the coefficient of electric current viscosity.