gebhart/patrepeval huggingface dataset:
Synthesis of the Ca2+-mobilizing messengers NAADP and cADPR by intracellular CD38 enzyme in the mouse heart: Role in β-adrenoceptor signaling (2017)

Nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR) are Ca<sup>2+</sup>-mobilizing messengers important for modulating cardiac excitation-contraction coupling and pathophysiology. CD38, which belongs to the ADP-ribosyl cyclase family, catalyzes synthesis of both NAADP and cADPR <i>in vitro</i> However, it remains unclear whether this is the main enzyme for their production under physiological conditions. Here we show that membrane fractions from WT but not <i>CD38</i><sup>-/-</sup> mouse hearts supported NAADP and cADPR synthesis. Membrane permeabilization of cardiac myocytes with saponin and/or Triton X-100 increased NAADP synthesis, indicating that intracellular CD38 contributes to NAADP production. The permeabilization also permitted immunostaining of CD38, with a striated pattern in WT myocytes, whereas <i>CD38</i><sup>-/-</sup> myocytes and nonpermeabilized WT myocytes showed little or no staining, without striation. A component of β-adrenoreceptor signaling in the heart involves NAADP and lysosomes. Accordingly, in the presence of isoproterenol, Ca<sup>2+</sup> transients and contraction amplitudes were smaller in <i>CD38</i><sup>-/-</sup> myocytes than in the WT. In addition, suppressing lysosomal function with bafilomycin A1 reduced the isoproterenol-induced increase in Ca<sup>2+</sup> transients in cardiac myocytes from WT but not <i>CD38</i><sup>-/-</sup> mice. Whole hearts isolated from <i>CD38</i><sup>-/-</sup> mice and exposed to isoproterenol showed reduced arrhythmias. SAN4825, an ADP-ribosyl cyclase inhibitor that reduces cADPR and NAADP synthesis in mouse membrane fractions, was shown to bind to CD38 in docking simulations and reduced the isoproterenol-induced arrhythmias in WT hearts. These observations support generation of NAADP and cADPR by intracellular CD38, which contributes to effects of β-adrenoreceptor stimulation to increase both Ca<sup>2+</sup> transients and the tendency to disturb heart rhythm.
Salvage Therapy of Open, Infected Surgical Wounds: A Retrospective Review Using Techni-Care ® (2000)

Despite severe underlying diseases, all patients were discharged from the hospital with closed or healing wounds. We recommend treatment with PCMX-PL as an adjunctive therapy for infected wounds particularly when standard care measures have failed.
SCALING LAW FOR FILAMENTARY SUPERCONDUCTOR HYSTERESIS LOSSES IN SUPERIMPOSED dc AND ac MAGNETIC FIELDS (1984)

Hyteresis losses of multifilament superconductors have been considered in transverse, superimposed ac and dc magnetic fields. These losses scale following a law in which the ratio of the ac field to the penetration field is the only important parameter. The advantages and the limits of such a scaling law are discussed.
Exploiting Emotional Information for Trust/Distrust Prediction (2016)

Trust and distrust networks are usually extremely sparse and the vast majority of the existing algorithms for trust/distrust prediction suffer from the data sparsity problem. In this paper, following the research from psychology and sociology, we envision that users' emotions such as happiness and anger are strong indicators of trust/distrust relations. Meanwhile the popularity of social media encourages the increasing number of users to freely express their emotions; hence emotional information is pervasively available and usually denser than the trust and distrust relations. Therefore incorporating emotional information could have the potentials to alleviate the data sparsity in the problem of trust/distrust prediction. In this study, we investigate how to exploit emotional information for trust/distrust prediction. In particular, we provide a principled way to capture emotional information mathematically and propose a novel trust/distrust prediction framework ETD. Experimental results on the real-world social media dataset demonstrate the effectiveness of the proposed framework and the importance of emotional information in trust/distrust prediction.
Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules (1989)

In an effort to extend our studies on Ca2+ pumps to animal models, we developed a new monoclonal antibody (5F10) prepared against the human erythrocyte Ca2+-Mg2+-adenosinetriphosphatase (ATPase) that recognizes a protein of approximately 140 kDa in rat kidney homogenates. Enzyme-linked immunosorbent assays show that monoclonal antibody 5F10 binds purified Ca2+-Mg2+-ATPase and rat kidney membrane extracts in a concentration-dependent manner. In paraffin-embedded tissue sections, antibody 5F10 binds to an epitope in the basolateral membranes of rat kidney distal convoluted tubule principal cells. The antibody does not bind to intercalated cells. The latter cells were characterized by the presence of large amounts of carbonic anhydrase C. Polyclonal antibodies directed against chick intestinal 28-kDa vitamin D-dependent calcium binding protein (28-kDa CaBP) also bind epitopes in distal convoluted tubule cells, connecting tubules, and portions of collecting duct but not intercalated cells. Western blot and 45Ca blot analysis of renal cytosolic proteins showed that the polyclonal 28-kDa CaBP-directed antibody detects a protein which also binds calcium. Western blot analysis with monoclonal antibody 5F10 shows binding to both the authentic purified erythrocyte Ca2+ pump (approximately 138 kDa) and to tryptic fragments of this pump. Antibody JA3, previously used for staining of human kidney tubules, reacts with a different set of tryptic fragments, showing that the two antibodies are directed against different regions or conformational determinants on the pump molecule. We show that Ca2+-Mg2+-ATPase and 28-kDa CaBP are present in the principal cells of the distal convoluted tubule of the rat and are absent in intercalated cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Vibrio vulnificus infection--report of 8 cases and review of cases in Taiwan. (1994)

Vibrio vulnificus infection, which is a rare and fatal disease, can be categorized clinically as either primary septicemia or wound infection. The clinical presentation of patients with primary septicemia can vary from fever alone to a more severe illness including high-grade bullous lesions, hypotension, and shock. Wound infection typically results from either injury to the skin in a marine environment or contact of a preexisting wound with sea water. We reported eight cases with Vibrio vulnificus infection in Chang gung Memorial Hospital and reviewed ten other cases previously reported with details in Taiwan. Fourteen patients presented with primary septicemia, and four with wound infection. Thirteen patients had alcoholism or chronic liver disease, two had peptic ulcer disease, one was steroids abuser, and one patient had thalassemia and chronic liver disease. Overall mortality was 55.6% (ten patients). Patients with hypotension within 48 hours of admission had higher mortality than normotensive patients (77% vs. 0%, P = 0.007). Patients with chronic liver disease or liver cirrhosis also had tendency to a higher mortality than not (64% vs. 25%, P = 0.274). Chronic liver diseases and liver cirrhosis are common disease in Taiwan. They take a high risk for Vibrio vulnificus infection. Clinician should keep in mind of this potentially fatal infection in these patients reporting a history of recent raw oyster consumption and presented with sepsis and characterized skin lesions. Prompt empirical antibiotics treatment and aggressive surgical treatment may be lifesaving for this acute and fatal disease.
A deficiency in Syk enhances ceramide‐induced apoptosis in DT40 lymphoma B cells (1998)

Syk deficiency significantly enhanced ceramide-induced apoptosis. Ectopic expression of wild-type or kinase-inactive Syk rendered Syk-negative cells resistant to ceramide-induced apoptosis. Furthermore, ceramide could not activate Syk, indicating that Syk protected DT40 cells from ceramide-induced apoptosis, via a mechanism independent of its activity. In addition, a deficiency in Lyn also resulted in the cells becoming susceptible to ceramide-induced apoptosis. However, no difference of Ara-C-induced apoptosis between wild-type and mutant cells was observed. c-Jun N-terminal kinases appeared not to be important in mediating the enhanced apoptosis, as they were still activated in mutant cells following ceramide treatment.
Identification of a point mutation and germinal mosaicism in a duchenne muscular dystrophy family (1994)

Duchenne and Becker muscular dystrophies (DMD and BMD) are allelic X-linked disorders arising from mutations in the (2.4 Mb) dystrophin gene at Xp21. We have applied the reverse transcriptase-polymerase chain reaction (RT-PCR) to identify a larger than normal dystrophin mRNA from a male with Duchenne muscular dystrophy and his younger affected brother. The increased size of the dystrophin mRNA was due to a splice-site mutation at the exon 26:intron 26 junction where a T to G substitution prevented normal RNA processing. A cryptic splice-site, downstream of the mutation, was activated during processing, resulting in the inclusion of 117 bases of intron 26. This insertion introduced an in-frame stop codon into the mature dystrophin mRNA. An allele-specific test was developed to identify the mutation and was applied to this family. Interestingly, the mother of the two affected boys did not carry the mutation, as determined by allele-specific amplification and direct DNA sequence analysis, indicating gonadal mosaicism. Her eldest daughter, designated as a carrier based upon conventional testing and haplotype analysis, also did not carry the family mutation. Initial haplotyping of the family appeared to be straightforward with gonadal mosaicism becoming evident only after allele-specific analysis. The application of linked markers to identify the disease allele for conventional genetic counselling would have been erroneous in this family and highlights the diagnostic power of precise identification of the disease-causing mutation.
Modern Adaptive Real-Time Controllers for Actively Reacting Flexible Structures (1991)

This paper describes modern controllers, which are based on digital real- time filters, powerful adaptation algorithms and high speed signal processor systems. Such controllers are required for active shape and vibration control on large flexible space structures.
Standardized ADOS Scores: Measuring Severity of Autism Spectrum Disorders in a Dutch Sample (2010)

The validity of the calibrated severity scores on the ADOS as reported by Gotham et al. (J Autism Dev Disord 39: 693-705, 2009), was investigated in an independent sample of 1248 Dutch children with 1455 ADOS administrations (modules 1, 2 and 3). The greater comparability between ADOS administrations at different times, ages and in different modules, as reached by Gotham et al. with the calibrated severity measures, seems to be corroborated by the current study for module 1 and to a lesser extent for module 3. For module 2, the calibrated severity scores need to be further investigated within a sample that resembles Gotham's sample in age and level of verbal functioning.
RISK MANAGEMENT FOR GRID COMPANIES - CHALLENGES WITH RESPECT TO ECONOMY AND SAFETY (2003)

This paper gives a brief description of risk elements the Norwegian grid companies are facing under the monopoly regime. Further some practical experiences from a grid company are given to illustrate how risk based methods are being applied in order to meet the incentives given by the regulatory authorities. The paper concludes with some future challenges in handling risk elements, with emphasis on how to take into account both economic and safety aspects when planning and operating the grid.
Physiological evaluation of temperature effect on the growth processes of the mysid, Neomysis intermedia Czerniawsky (1987)

Ingestion, respiration, and molting loss rates were measured over the 3 – 29°C range in Neomysis intermedia . Weight specific rates of these physiological processes ranged from 2 to 140% body C day −1 for ingestion, from 2 to 15% body C day −1 for respiration, and from 0.1 to 5% body C day −1 for molting loss. All weight-specific rates showed a logarithmic decrease with a logarithmic increase in body weight, and a logarithmic increase with a linear increase in temperature below 20 or 25°C. The effect of temperature, however, was different between the physiological rates, with a large temperature dependency for ingestion ( Q10 = 2.6 –3.9) and molting loss ( Q10 = 2.9 – 3.6) and a moderate temperature dependency for respiration ( Q10 = 1.9 – 2.1). Calculated assimilation efficiency changed with body size, but was constant over the temperature range examined. Allocation of assimilated materials varied with a change in temperature, reflecting the different temperature dependence between physiological processes. It was deduced that the strong temperature dependency of the growth rate in N. intermedia observed in the previous studies resulted from the large temperature effect on ingestion and assimilation rates, superimposed by the different allocation of assimilated materials.
Ca2+-dependent Conformational Changes in a C-terminal Cytosolic Domain of Polycystin-2 (2009)

The PKD1 and PKD2 genes are the genes that are mutated in patients suffering from autosomal dominant polycystic kidney disease. The human PKD2 gene codes for a 968-amino acid long membrane protein called polycystin-2 that represents a cation channel whose activity can be regulated by Ca(2+) ions. By CD, fluorescence, and NMR spectroscopy, we have studied a 117-amino acid-long fragment of the cytoplasmic domain of polycystin-2, polycystin-2-(680-796) that was proposed to contain a Ca(2+)-binding site. NMR structure determination reveals the existence of two Ca(2+)-binding sites in polycystin-2-(680-796) arranged in a typical and an atypical EF-hand motif. In the absence of Ca(2+) the protein forms a dimer that is dissociated by Ca(2+) binding. This dissociation may be related to the Ca(2+) inactivation observed earlier. The calcium affinity of the protein was determined by fluorescence and NMR spectroscopy. At 293 K, the K(D) values for the high and low affinity sites are 55 mum and 179 mum, respectively.
Dietary Supplement Use and Prostate Cancer Risk in the Carotene and Retinol Efficacy Trial (2009)

We investigated dietary supplement use and prostate cancer risk in the Carotene and Retinol Efficacy Trial (CARET). CARET was a randomized, double-blinded, placebo-controlled trial testing a daily dose of 30 mg beta-carotene + 25,000 IU retinyl palmitate for lung cancer prevention (1985-1996; active follow-up occurred through 2005). Secondary outcomes, including prostate cancer, were also assessed. Participants were queried about dietary supplements, health history, family history of cancer, smoking, and lifestyle habits. Cox proportional hazards regression estimated multivariate-adjusted relative risk [and 95% confidence intervals (95% CI)] of prostate cancer for dietary supplement users and nonusers with or without the high-dose CARET vitamins during the intervention and postintervention phases. After an average of 11 years of follow-up, 890 prostate cancer cases were reported. Neither the CARET nor other supplements were associated with total prostate cancer risk. For aggressive prostate cancer, men in the CARET intervention arm who used additional supplements had a relative risk for aggressive prostate cancer (Gleason >or=7 or stage III/IV) of 1.52 (95% CI, 1.03-2.24; P < 0.05), relative to all others. These associations disappeared in the postintervention period (0.75; 95% CI, 0.51-1.09). Conversely, there was no association of CARET + other supplements with nonaggressive disease, relative to all others. There was no effect modification by smoking or time on CARET intervention in any analyses. CARET only included smokers, so findings reported here may not apply to nonsmokers. Our results are consistent with other studies suggesting that dietary supplements may influence prostate cancer risk.
Transgenic Expression of Cacna1f Rescues Vision and Retinal Morphology in a Mouse Model of Congenital Stationary Night Blindness 2A (CSNB2A) (2020)

These data have relevance in the treatment of CSNB2A and in understanding how photoreceptor integration might be achieved in retinas in which photoreceptors have been lost, such as retinitis pigmentosa, age-related macular degeneration, and other degenerative conditions.
Highly luminescent InP/GaP/ZnS QDs emitting in the entire color range via a heating up process (2016)

InP-based quantum dots (QDs) have attracted much attention for use in optical applications, and several types of QDs such as InP/ZnS, InP/ZnSeS, and InP/GaP/ZnS have been developed. However, early synthetic methods that involved rapid injection at high temperatures have not been able to reproducibly produce the required optical properties. They were also not able to support commercialization efforts successfully. Herein, we introduce a simple synthetic method for InP/GaP/ZnS core/shell/shell QDs via a heating process. The reaction was completed within 0.5 h and a full color range from blue to red was achieved. For emitting blue color, t-DDT was applied to prevent particle growth. From green to orange, color variation was achieved by adjusting the quantity of myristic acid. Utilizing large quantities of gallium chloride led to red color. With this method, we produced high-quality InP/GaP/ZnS QDs (blue QY: ~40%, FWHM: 50 nm; green QY: ~85%, FWHM: 41 nm; red QY: ~60%, FWHM: 65 nm). We utilized t-DDT as a new sulfur source. Compared with n-DDT, t-DDT was more reactive, which allowed for the formation of a thicker shell.
Antagonistic Effect of C/EBPα and Pax5 in Myeloid or Lymphoid Lineage Choice in Common Lymphoid Progenitors. (2005)

Abstract Lymphoid lineage committed progenitors, such as common lymphoid progenitors (CLPs), maintain a latent myeloid differentiation potential, which can be initiated by stimulation through exogenously expressed cytokine receptors including IL-2 receptors. The transcription factor C/EBPa is promptly upregulated in CLPs upon ectopic IL-2 stimulation. We show that enforced C/EBPa expression is sufficient to initiate myeloid differentiation from CLPs, as well as from proT and proB cells, even though proB cells do not give rise to myeloid cells following ectopic IL-2 stimulation. Expression of Pax5, a B lymphoid affiliated transcription factor, is completely suppressed by enforced C/EBPa but not by ectopic IL-2 stimulation in proB cells. Introduction of Pax5 blocks ectopic IL-2 receptor-mediated myeloid lineage conversion in CLPs. These data suggest that C/EBPa is a proximal target of cytokine induced lineage conversion in lymphoid progenitors. Furthermore, complete loss of Pax5 expression triggered by upregulation of C/EBPa is a critical event for lineage conversion from lymphoid to myeloid lineage in CLPs and proB cells.
For your eye only (2009)

A contact lens with one LED that is powered wirelessly with radiofrequency (RF) is built. The conventional contact lenses made up of polymers are integrated with control circuits, communication circuits, and miniature antennas using custom-built optoelectronic components that include hundreds of LEDs. The lenses are successfully tested with live rabbits and can be a multipurpose, whether used as biosensors, displays, etc.
<i>abo8</i> mutants are hypersensitive to ABA in seed germination and root growth. (2015)

&lt;p&gt;A. The root growth phenotype of &lt;i&gt;abo8&lt;/i&gt; mutants on MS medium or MS medium supplemented with different concentrations of ABA. Four-day-old seedlings grown on MS were transferred to MS medium containing 0, 10, or 30 µM ABA for 5 days before they were photographed. Bars = 5 mm. B. Statistical analysis of the root length in different growing times. C. Relative root growth. Root length is expressed relative to that of the wild type or &lt;i&gt;abo8&lt;/i&gt; mutants without ABA. In (B) and (C), three independent experiments were done with similar results, each with three biological repeats. Five roots from one plate were measured for each repeat. Values are means ±SE, n = 3. **: P&lt;0.01. D. Seed germination greening of &lt;i&gt;abo8&lt;/i&gt; mutants after 7 days on MS medium supplemented with 0.0, 0.3, or 0.5 µM ABA. Bars = 2 mm. E. Statistical analysis of seed germination greening rate in (D). Three independent experiments were done, each with three replicates (from three different plates). About 24 seeds were counted for each plate. Values are means ±SE, n = 3. **: P&lt;0.01.&lt;/p&gt;
Ultrathin g-C3N4/TiO2composites as photoelectrochemical elements for the real-time evaluation of global antioxidant capacity (2014)

Using utg-C<sub>3</sub>N<sub>4</sub>/TiO<sub>2</sub>, a photoelectrochemical platform was designed for the sensing of global antioxidant capacity, which presented a rapid response, and anti-fouling and colour-interference-proof properties.
Variation in the vulnerability of mice expressing human superoxide dismutase 1 to prion-like seeding: a study of the influence of primary amino acid sequence (2021)

Misfolded forms of superoxide dismutase 1 (SOD1) with mutations associated with familial amyotrophic lateral sclerosis (fALS) exhibit prion characteristics, including the ability to act as seeds to accelerate motor neuron disease in mouse models. A key feature of infectious prion seeding is that the efficiency of transmission is governed by the primary sequence of prion protein (PrP). Isologous seeding, where the sequence of the PrP in the seed matches that of the host, is generally much more efficient than when there is a sequence mis-match. Here, we used paradigms in which mutant SOD1 seeding homogenates were injected intraspinally in newborn mice or into the sciatic nerve of adult mice, to assess the influence of SOD1 primary sequence on seeding efficiency. We observed a spectrum of seeding efficiencies depending upon both the SOD1 expressed by mice injected with seeds and the origin of the seed preparations. Mice expressing WT human SOD1 or the disease variant G37R were resistant to isologous seeding. Mice expressing G93A SOD1 were also largely resistant to isologous seeding, with limited success in one line of mice that express at low levels. By contrast, mice expressing human G85R-SOD1 were highly susceptible to isologous seeding but resistant to heterologous seeding by homogenates from paralyzed mice over-expressing mouse SOD1-G86R. In other seeding experiments with G85R SOD1:YFP mice, we observed that homogenates from paralyzed animals expressing the H46R or G37R variants of human SOD1 were less effective than seeds prepared from mice expressing the human G93A variant. These sequence mis-match effects were less pronounced when we used purified recombinant SOD1 that had been fibrilized in vitro as the seeding preparation. Collectively, our findings demonstrate diversity in the abilities of ALS variants of SOD1 to initiate or sustain prion-like propagation of misfolded conformations that produce motor neuron disease.
A New Test Facility for Testing of Cooled Gasturbine Components (1998)

A high pressure hot test facility for cooled gas turbine components has been developed for use in turbine cooling research. In this facility, heat transfer tests for a sector of real turbine vanes can be performed under continuous operation. The heat transfer tests are performed at an operating point that is scaled down from the real engine operating point. The compressor can deliver air at the rate of up to 10 kg/s at 20 bars. Air temperatures of up to 1170 K can be achieved by using an oil-fired combustor. Besides conventional instrumentation such as thermocouples and pressure probes, the facility is equipped with an IR-camera to map two-dimensional wall temperature fields. Hot wire anemometry and an LDV system are used to determine mean and fluctuating velocity components. This paper describes design and performance of the test facility as well as the control and measurement equipment. The test and evaluation procedures used for testing of cooled gas turbine vanes are also presented.