gebhart/patrepeval huggingface dataset:
Motion and gravitational wave forms of eccentric compact binaries with orbital-angular-momentum-aligned spins under next-to-leading order in spin–orbit and leading order in spin(1)–spin(2) and spin-squared couplings (2010)

A quasi-Keplerian parameterisation for the solutions of second post-Newtonian (PN) accurate equations of motion for spinning compact binaries is obtained including leading order spin-spin and next-to-leading order spin-orbit interactions. Rotational deformation of the compact objects is incorporated. For arbitrary mass ratios the spin orientations are taken to be parallel or anti-parallel to the orbital angular momentum vector. The emitted gravitational wave forms are given in analytic form up to 2PN point particle, 1.5PN spin orbit and 1PN spin-spin contributions, where the spins are counted of 0PN order.
Anisotropic fibre-reinforced plastics as formworks for single and double-curved textile reinforced concrete (2014)

A new constructive and technological approach was developed for the efficient production of large-dimensioned, curved freeform formworks, which allows the manufacturing of single and double-curved textile reinforced concrete elements. The approach is based on a flexible, multi-layered formwork system, which consists of glass-fibre reinforced plastic (GFRP). Using the unusual structural behavior caused by anisotropy, these GFRP formwork elements permit a specific adjustment of defined curvature. The system design of the developed GFRP formwork and the concrete-lightweight-elements with stabilized spacer fabric was examined exhaustively. Prototypical curved freeform surfaces with different curvature radii were designed, numerically computed and produced. Furthermore, the fabric’s contour accuracy of the fabric was verified, and its integration was adjusted to loads. The developed textile reinforced concrete (TRC) had 3-point bending tensile strength of 41.51 MPa. Beyond that, it was ensured that the TRC had a high durability, which has been shown by the capillary suction of de-icing solution and freeze thaw test with a total amount of scaled material of 1172 g/m² and a relative dynamic E-Modulus of 100% after 28 freeze-thaw cycles.
Comparison of the Adaptive Optics Vision Analyzer and the KR‐1 W for measuring ocular wave aberrations (2016)

The results suggest good agreement between instruments. Accommodation and misalignment of the measurements may play a role in some of the statistically significant differences that were obtained, specifically for defocus C(2,0), vertical coma C(3,-1) and spherical aberration C(4,0) coefficients; however, these differences were clinically irrelevant.
Novel CNBP‐ and La‐based translation control systems for mammalian cells (2002)

Throughout the development of Xenopus, production of ribosomal proteins (rp) is regulated at the translational level. Translation control is mediated by a terminal oligopyrimidine element (TOP) present in the 5' untranslated region (UTR) of rp-encoding mRNAs. TOP elements adopt a specific secondary structure that prevents ribosome-binding and translation-initiation of rp-encoding mRNAs. However, binding of CNBP (cellular nucleic acid binding protein) or La proteins to the TOP hairpin structure abolishes the TOP-mediated transcription block and induces rp production. Based on the specific CNBP-TOP/La-TOP interactions we have designed a translation control system (TCS) for conditional as well as adjustable translation of desired transgene mRNAs in mammalian cells. The generic TCS configuration consists of a plasmid encoding CNBP or La under control of the tetracycline-responsive expression system (TET(OFF)) and a target expression vector containing a TOP module between a constitutive P(SV40) promoter and the human model product gene SEAP (human secreted alkaline phosphatase) (P(SV40)-TOP-SEAP-pA). The TCS technology showed excellent SEAP regulation profiles in transgenic Chinese hamster ovary (CHO) cells. Alternatively to CNBP and La, TOP-mediated translation control can also be adjusted by artificial phosphorothioate anti-TOP oligodeoxynucleotides. Confocal laser-scanning microscopy demonstrated cellular uptake of FITC-labeled oligodeoxynucleotides and their localization in perinuclear organelles within 24 hours. Besides their TOP-based translation-controlling capacity, CNBP and La were also shown to increase cap-independent translation from polioviral internal ribosomal entry sites (IRES) and La alone to boost cap-dependent translation initiation. CNBP and La exemplify for the first time the potential of RNA-binding proteins to exert translation control of desired transgenes and to increase heterologous protein production in mammalian cells. We expect both of these assets to advance current gene therapy and biopharmaceutical manufacturing strategies.
Fibrous Organosilicon Polymers Derived from Silicates (1982)

A new, inherently fibrous organosilicon polymer having pendent trimethylsilyl groups is reported. It has been made from the rare ladder or tube silicate litidionite using a Lentz-type extraction-substitution process. The constituent fibers of this polymer have very small diameters, often 40-60 Å, and are flexible and inert. They are believed to have frameworks closely related to that of the parent silicate ion. A polymer which appears to be the same as this one has been made from the synthetic silicate Na2CuSi4O10, a close structural analog of litidionite. In addition a related polymer carrying dimethylvinyl groups has been made from litidionite by a like Lentz-type procedure. Both silicates have been made by simple thermal procedures.
Biochemical characterization of rice trehalose‐6‐phosphate phosphatases supports distinctive functions of these plant enzymes (2007)

Substantial levels of trehalose accumulate in bacteria, fungi, and invertebrates, where it serves as a storage carbohydrate or as a protectant against environmental stresses. In higher plants, trehalose is detected at fairly low levels; therefore, a regulatory or signaling function has been proposed for this molecule. In many organisms, trehalose-6-phosphate phosphatase is the enzyme governing the final step of trehalose biosynthesis. Here we report that OsTPP1 and OsTPP2 are the two major trehalose-6-phosphate phosphatase genes expressed in vegetative tissues of rice. Similar to results obtained from our previous OsTPP1 study, complementation analysis of a yeast trehalose-6-phosphate phosphatase mutant and activity measurement of the recombinant protein demonstrated that OsTPP2 encodes a functional trehalose-6-phosphate phosphatase enzyme. OsTPP2 expression is transiently induced in response to chilling and other abiotic stresses. Enzymatic characterization of recombinant OsTPP1 and OsTPP2 revealed stringent substrate specificity for trehalose 6-phosphate and about 10 times lower K(m) values for trehalose 6-phosphate as compared with trehalose-6-phosphate phosphatase enzymes from microorganisms. OsTPP1 and OsTPP2 also clearly contrasted with microbial enzymes, in that they are generally unstable, almost completely losing activity when subjected to heat treatment at 50 degrees C for 4 min. These characteristics of rice trehalose-6-phosphate phosphatase enzymes are consistent with very low cellular substrate concentration and tightly regulated gene expression. These data also support a plant-specific function of trehalose biosynthesis in response to environmental stresses.
Active-feedback frequency-compensation technique for low-power multistage amplifiers (2003)

An active-feedback frequency-compensation (AFFC) technique for low-power operational amplifiers is presented in this paper. With an active-feedback mechanism, a high-speed block separates the low-frequency high-gain path and high-frequency signal path such that high gain and wide bandwidth can be achieved simultaneously in the AFFC amplifier. The gain stage in the active-feedback network also reduces the size of the compensation capacitors such that the overall chip area of the amplifier becomes smaller and the slew rate is improved. Furthermore, the presence of a left-half-plane zero in the proposed AFFC topology improves the stability and settling behavior of the amplifier. Three-stage amplifiers based on AFFC and nested-Miller compensation (NMC) techniques have been implemented by a commercial 0.8-μm CMOS process. When driving a 120-pF capacitive load, the AFFC amplifier achieves over 100-dB dc gain, 4.5-MHz gain-bandwidth product (GBW) , 65° phase margin, and 1.5-V/μs average slew rate, while only dissipating 400-μW power at a 2-V supply. Compared to a three-stage NMC amplifier, the proposed AFFC amplifier provides improvement in both the GBW and slew rate by 11 times and reduces the chip area by 2.3 times without significant increase in the power consumption.
Development of a 384-Well Colorimetric Assay to Quantify Hydrogen Peroxide Generated by the Redox Cycling of Compounds in the Presence of Reducing Agents (2008)

We report here the development and optimization of a simple 384-well colorimetric assay to measure H(2)O(2) generated by the redox cycling of compounds incubated with reducing agents in high-throughput screening (HTS) assay buffers. The phenol red-horseradish peroxidase (HRP) assay readily detected H(2)O(2) either added exogenously or generated by the redox cycling of compounds in dithiothreitol (DTT). The generation of H(2)O(2) was dependent on the concentration of both the compound and DTT and was abolished by catalase. Although both DTT and tris(2-carboxyethyl) phosphine sustain the redox cycling generation of H(2)O(2) by a model quinolinedione, 6-chloro-7-(2-morpholin-4-yl-ethylamino)-quinoline-5,8-dione (NSC 663284; DA3003-1), other reducing agents such as beta-mercaptoethanol, glutathione, and cysteine do not. The assay is compatible with HTS. Once terminated, the assay signal was stable for at least 5 h, allowing for a reasonable throughput. The assay tolerated up to 20% dimethyl sulfoxide, allowing a wide range of compound concentrations to be tested. The assay signal window was robust and reproducible with average Z-factors of > or =0.8, and the redox cycling generation of H(2)O(2) by DA3003-1 in DTT exhibited an average 50% effective concentration of 0.830 +/- 0.068 microM. Five of the mitogen-activated protein kinase phosphatase (MKP) 1 inhibitors identified in an HTS were shown to generate H(2)O(2) in the presence of DTT, and their inhibition of MKP-1 activity was shown to be time dependent and was abolished or significantly reduced by either 100 U of catalase or by higher DTT levels. A cross-target query of the PubChem database with three structurally related pyrimidotriazinediones revealed active flags in 36-39% of the primary screening assays. Activity was confirmed against a number of targets containing active site cysteines, including protein tyrosine phosphatases, cathepsins, and caspases, as well as a number of cellular cytotoxicity assays. Rather than utilize resources to conduct a hit characterization effort involving several secondary assays, the phenol red-HRP assay provides a simple, rapid, sensitive, and inexpensive method to identify compounds that redox cycle in DTT or tris(2-carboxyethyl)phosphine to produce H(2)O(2) that may indirectly modulate target activity and represent promiscuous false-positives from a primary screen.
Characterization of Morphology and Mechanical Properties of Glass Interior Irradiated by Femtosecond Laser (2010)

Femtosecond laser pulses were focused in the interior of a single fused silica piece. Proper use of optical and laser processing parameters generated structural rearrangement of the material through a thermal accumulation mechanism, which could be potentially used for the transmission welding process. The morphology of generated features was studied using differential interference contrast optical microscopy. In addition, the predictive capability of the morphology is developed via a finite element analysis. The change in mechanical properties was studied through employment of spatially resolved nanoindentation. The specimen was sectioned and nanoindents were applied at the cross section to examine mechanical responses of the laser-modified region. Fracture toughness measurements are carried out to investigate the effects of the laser treatment on strength of the glass.
Cherenkov radiation from electrons passing through human tissue (2016)

A method for investigating the optical properties of human tissues is suggested. The method is based on the measurement of Cherenkov radiation produced by relativistic electrons passing through the tissue. Monte-Carlo simulation of visible photon emission and propagation is carried out taking into account multiple electron and photon scattering processes. Sensitivity of the Cherenkov radiation to the optical characteristics of human tissues is demonstrated.
Platinum(II) O,S Complexes Inhibit the Aggregation of Amyloid Model Systems (2019)

Platinum(II) complexes with different cinnamic acid derivatives as ligands were investigated for their ability to inhibit the aggregation process of amyloid systems derived from Aβ, Yeast Prion Protein Sup35p and the C-terminal domain of nucleophosmin 1. Thioflavin T binding assays and circular dichroism data indicate that these compounds strongly inhibit the aggregation of investigated peptides exhibiting IC<sub>50</sub> values in the micromolar range. MS analysis confirms the formation of adducts between peptides and Pt(II) complexes that are also able to reduce amyloid cytotoxicity in human SH-SY5Y neuroblastoma cells. Overall data suggests that bidentate ligands based on β-hydroxy dithiocinnamic esters can be used to develop platinum or platinoid compounds with anti-amyloid aggregation properties.
Alkylsulfamoyl Chlorides as Key Units in the Synthesis of Novel Biologically Active Compounds for Crop Protection (1981)

Abstract Due to their bifunctional character, alkylsulfamoyl chlorides are versatile units for the synthesis of heterocycles, polar sulfamates, and sulfonamides. In the last decade, synthetic methods of general preparative use have been developed, by means of which amine hydrochlorides, isocyanates, aziridines or tertiary alcohols can be reacted with suitable sulfuric acid derivatives to give novel, variously substituted alkylsulfamoyl chlorides. These compounds can subsequently be converted either to previously unobtainable N ‐alkoxyalkyl‐ N ‐alkylsulfamoyl chlorides or to novel heterocycles of the type 1 H ‐2,1,3‐benzothiadiazin‐4‐one‐2,2‐dioxide, 2 H ‐1,2,6‐thiadia‐zin‐3‐one‐1,1‐dioxide and 2 H ‐1,2,4,6‐thiatriazin‐5‐one‐1,1‐dioxide; these compounds are examples of interesting models which illustrate the relation between the structure and the action of the compound, and in some cases lead to highly selective, ecologically unobjectionable herbicides. On the other hand, the alkylsulfamoyl chlorides themselves can be N ‐acylated to give further 3‐ to 5‐atom bifunctional synthesis units, with which novel heterocyclic syntheses can be carried out. Further uses of the alkylsulfamoyl chlorides include the preparation of biologically active sulfamates, and cycloaddition reactions of N ‐sulfonylamines prepared in situ .
Corneal epithelial limbal stem cell transplantation for ocular surface reconstruction (2011)

Abstract Abstract not provided
Estimation of ground reaction force waveforms during fixed pace running outside the laboratory (2023)

In laboratory experiments, biomechanical data collections with wearable technologies and machine learning have been promising. Despite the development of lightweight portable sensors and algorithms for the identification of gait events and estimation of kinetic waveforms, machine learning models have yet to be used to full potential. We propose the use of a Long Short Term Memory network to map inertial data to ground reaction force data gathered in a semi-uncontrolled environment. Fifteen healthy runners were recruited for this study, with varied running experience: novice to highly trained runners (<15 min 5 km race), and ages ranging from 18 to 64 years old. Force sensing insoles were used to measure normal foot-shoe forces, providing the standard for identification of gait events and measurement of kinetic waveforms. Three inertial measurement units (IMUs) were mounted to each participant, two bilaterally on the dorsal aspect of the foot and one clipped to the back of each participant's waistband, approximating their sacrum. Data input into the Long Short Term Memory network were from the three IMUs and output were estimated kinetic waveforms, compared against the standard of the force sensing insoles. The range of RMSE for each stance phase was from 0.189-0.288 BW, which is similar to multiple previous studies. Estimation of foot contact had an <i>r</i> <sup>2</sup> = 0.795. Estimation of kinetic variables varied, with peak force presenting the best output with an <i>r</i> <sup>2</sup> = 0.614. In conclusion, we have shown that at controlled paces over level ground a Long Short Term Memory network can estimate 4 s temporal windows of ground reaction force data across a range of running speeds.
Possible Association between Expression of Chemokine Receptor-2 (CCR2) and Amyotrophic Lateral Sclerosis (ALS) Patients of North India (2012)

Downregulation of PBMCs CCR2 may indicate its etio-pathological relevance in ALS pathogenesis. Reduced PBMCs CCR2 may result in decreased infiltration of leukocytes at the site of degeneration as a compensatory response to ALS. CCR2 levels measurements in hematopoietic stem cells and estimation of comparative PBMCs count among ALS, disease controls and normal controls can unveil its direct neuroprotective role. However, the conclusions are restricted by the absence of neurological/non-neurological disease controls in the study.
Renforcement des interactions nonlinéaires par excitation cohérente d’une micro-cavité (orale) (2016)

National audience
Intraepithelial T cell phenotype and function diverge by intestinal location with age (2020)

Abstract Intestinal intraepithelial T cells (IETs) provide a first line of defense against intestinal pathogens and play an important role in immune tolerance versus activation. In pigs, intestinal T cell populations stabilize at ~8 weeks of age, but IET phenotypes and function are poorly understood during the early postnatal period. Better understanding age- and location-dependent characteristics of porcine IETs may be useful in developing strategies to maintain intestinal integrity and improve health. To assess impacts of age and intestinal location on abundance and composition of IETs, sections of jejunum, ileum, cecum, and colon from 4-, 6-, and 8-week-old pigs were analyzed by histologic staining and flow cytometric analyses. IET abundance increased with age, and CD2+CD8α+γδ and CD4−CD8α+αβ T cells were the primary IET populationsin all samples (&amp;gt;78%). Compositions of IETs were similar at all locations in 4-week-old pigs. As pigs aged, cecal and colonic IET compositions remained similar to each other but diverged from small intestinal IETs, while jejunal and ileal IET compositions diverged from both large intestinal IETs and each other. These findings indicate both intestinal location and age are drivers of IET heterogeneity. Small intestinal IETs had lower percentages of γδ IETs in 6- and 8-week-old pigs, and CD4−CD8α+αβ IETs increased in abundance at all intestinal locations as pigs aged. Percentages of CD27+IETs in ileum and large intestine decreased over time, but decreases were more drastic in large intestine, suggesting IETs in the distal but not proximal intestinal tract were activated as pigs aged. Small intestinal IET compositions closely resembled human IET populations, highlighting pigs as a biomedical model for IET research.
Efficient Expression, Purification and Functional Evaluation of Human Epidermal Growth Factor-Collagen Binding Domain Hybrid Protein on Growth Stimulation of Human Fibroblast Cells (2019)

Growth factors such as human epidermal growth factor (hEGF) have recently received high interest in regenerative medicine and pharmaceutical industries mainly due to their ability to restore tissues proliferation and improvement of their biological functions. In spite of various hEGF applications, its efficient expression in Escherichia coli could not yet reach an industrial reality mainly due to the lack of the ability of folding into the correct 3D structure because of three disulfide bonds in monomer hEGF. To address these challenges, here a fusion hEGF protein with a C terminus of collagen binding domain (CBD) along with intein protein with self-splicing property and ELP sequence was constructed by a three-step cloning procedure. This enabled us to purify recombinant hEGF without using chromatography columns. Following the confirmation of the construct by colony PCR, restriction enzymes analysis and sequencing, the 62[Formula: see text]kDa band of ELP-INTEIN-hEGF-CBD were observed on SDS-PAGE and confirmed by western blotting. Subsequently, the mitotic activity in Balb/c 3T3 cells proliferation in presence of recombinant hEGF-CBD compared with commercial hEGF using methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay which showed that our purified recombinant protein stimulates the cell proliferation similar to the commercial protein. Our strategy could be considered as a new feasible approach to produce hEGF in E. coli for pharmaceutical and clinical applications.
In-Process Monitoring of Continuous Fiber Additive Manufacturing through Force/Torque Sensing on the Nozzle (2020)

Additive manufacturing with continuous fiber reinforcement through the Fused Filament Fabrication process is an emerging composite manufacturing technology that can be used to manufacture functional parts. Parts are made through a continuous addition of reshaped, re-melted feedstock. In the case of continuous fiber additive processes, the continuous fiber is a solid connection between the nozzle and the part. This means that process variations may exert a force between the part and the deposition head. Excessive forces can result in a part defect, such as fiber pullout. In this paper, a prototype of a solution for monitoring the printing process during continuous fiber fused filament fabrication is presented. If the forces are excessive, fiber pullout or shearing may occur, leading a failed print or part defects. To monitor this, a force-torque sensor was implemented on the head of a 3-axis continuous fiber additive manufacturing platform. By recording and timing the measured force and torque data from the sensor onto the numerical code of the printed part, a force and torque intensity map can be generated on the geometry. This projection is adjusted for the nominal, expected, shear forces on the nozzle during the printing process. Any anomalies or out-ofbound conditions can be used both in real-time and off-line to quantify process health,consistency and reliability. The functionality of mapping nozzle forces and torques on a part is demonstrated for several test cases in this manuscript.
Prevalência de alta reatividade plaquetária em pacientes tratados com aspirina encaminhados para angiografia coronária (2013)

In a general population of ASA-treated patients referred for coronary angiography, elevated platelet reactivity is prevalent (13%) with 50% related to noncompliance and 50% related to reduced aspirin efficacy.