gebhart/patrepeval huggingface dataset:
Caracterização preliminar de um paramixovírus contaminante de cultivos celulares (2018)

Este artigo relata a caracterização preliminar de um vírus detectado como contaminante de cultivos celulares no Setor de Virologia da UFSM. O agente foi inicialmente detectado em células de linhagem de rim fetal bovino (MDBK) e posteriormente disseminou-se para cultivos celulares de outras espécies. A citopatologia, caracterizada por arredondamento celular, citomegalia, desprendimento e formação de sincícios era progressiva e resultava em morte celular e destruição do tapete em poucos dias. Exames iniciais de microscopia eletrônica (ME) de células afetadas revelaram a presença de partículas víricas semelhantes a herpesvírus. No entanto, testes de imunofluorescência e soro-neutralização não revelaram relação antigênica entre o agente e três herpesvírus humanos e dez de animais. Testes subseqüentes demonstraram que o agente possui um ciclo replicativo curto; atividade hemaglutinante com eritrócitos de ovinos e camundongos; e é capaz de replicar e produzir citopatologia em cultivos celulares de humanos e de várias espécies animais. Testes de imunofluorescência revelaram que o agente é relacionado antigenicamente com o vírus da parainfluenza tipo-3 humana (hPI-3) e bovina (bPI-3). Posteriormente, anticorpos com atividade neutralizante contra o agente foram detectados no soro de bovinos (715/847), ovinos (41/59) e humanos (27/95). Uma análise posterior das fotos de ME revelou a presença de partículas víricas e nucleocapsídeos helicoidais compatíveis com paramyxovírus. Esses resultados indicam que um vírus antigenicamente relacionado ao hPI-3 e bPI-3 está associado com a citopatologia observada nos cultivos. A identidade, espécie de origem do agente e a forma de contaminação dos cultivos estão sob investigação. A ocorrência dessa contaminação reforça a necessidade da adoção de medidas rígidas de biossegurança no manuseio de cultivos celulares, pelo risco de amplificação de vírus adventícios.
A method for selection of hybridomas, secreting monoclonal antibodies against tyrosyl-tRNA synthetase, based on monitoring of the enzymatic activity (1990)

Получена линия гибридом T₃, секретирующая моноклональные антитела к тирозил-тРНК синтетазе (КФ 6.1.1.1) из печени быка, с использованием метода селекции гибридом, основанного на определении ферментативной активности. Методом иммуноблотинга показано, что антигенная детерминанта для этих антител сохраняется в денатурирующих условиях.
Commentary (1996)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Ophthalmology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Ophthalmology journal
Efficient Quantum Pseudorandomness (2016)

Randomness is both a useful way to model natural systems and a useful tool for engineered systems, e.g., in computation, communication, and control. Fully random transformations require exponential time for either classical or quantum systems, but in many cases pseudorandom operations can emulate certain properties of truly random ones. Indeed, in the classical realm there is by now a well-developed theory regarding such pseudorandom operations. However, the construction of such objects turns out to be much harder in the quantum case. Here, we show that random quantum unitary time evolutions ("circuits") are a powerful source of quantum pseudorandomness. This gives for the first time a polynomial-time construction of quantum unitary designs, which can replace fully random operations in most applications, and shows that generic quantum dynamics cannot be distinguished from truly random processes. We discuss applications of our result to quantum information science, cryptography, and understanding the self-equilibration of closed quantum dynamics.
The Radiolysis of Pancreatic Ribonuclease: Kinetic and Optical Properties (1965)

Experiments have been undertaken in this laboratory to investigate the effects of Xand y-radiations on dilute aqueous solutions of bovine pancreatic ribonuclease (RNase).2 This enzyme offers several advantages in the investigation of structural changes accompanying radiolytic degradation. The covalent structure of the protein molecule has been completely determined (1), and it has been established that its secondary and tertiary structure is defined by the primary sequence of amino acids only (2). In the following report the effects of X-rays on the kinetic and optical properties of ribonuclease solutions are examined in relation to changes in the enzymatic activity.
In Vitro Self-Training in the Surgical Technique of Aortic Valve Repair and Reconstruction (1998)

A simple device for in vitro assessment of aortic surgery on an isolated heart or aortic root specimen is described. This device was designed as a simple tool to provide surgeons with immediate and direct visual feedback on the results of a procedure performed on the aortic valve in an isolated cardiac specimen. The objectives were to assess the duration of learning new aortic surgical procedures and to instill a greater sense of confidence to the surgeon prior to operating on a patient. Free-hand pericardial aortic valve reconstructions were performed on 9 pig hearts and 5 human homografts, which were tested with this device. In the pig, valve reconstruction was tested using the whole heart as well as the isolated aortic root. Detailed qualitative and quantitative assessments of the performance of the reconstructed aortic valve were possible using this device.
Application of nonlinear system identification to magnetic resonance imaging and computed tomography (2002)

The authors have examined the nonlinear behavior of MRI and CT using the Wiener-Volterra model of nonlinear systems, which allows evaluation of the type and quantitative significance of nonlinearities. The calculated nonlinear kernels revealed at least second order quantitatively significant nonlinearities in both CT and MRI related to spatial factors, such as geometric distortions and edge effects, and grey map transformations between modalities. Kernel calculations were complicated in CT image transformation mappings by partial volume effects, and in MRI mappings by magnetic susceptibility related image distortion.
Unsupervised Online Adaptation for Speaker Verification Over The Telephone (2004)

This paper presents experiments of unsupervised adaptation for a speaker detection system. The system used is a standard speaker verification system based on cepstral features and Gaussian mixture models. Experiments were performed on cellular speech data taken from the NIST 2002 speaker detection evaluation. There was a total of about 30.000 trials involving 330 target speakers and more than 90% of impostor trials. Unsupervised adaptation significantly increases the system accuracy, with a reduction of the minimal detection cost function (DCF) from 0.33 for the baseline system to 0.25 with unsupervised online adaptation. Two incremental adaptation modes were tested, either by using a fixed decision threshold for adaptation, or by using the a posteriori probability of the true target for weighting the adaptation. Both methods provide similar results in the best configurations, but the latter is less sensitive to the actual threshold value.
Fibroblastic niches prime T cell alloimmunity through Delta-like Notch ligands (2017)

Alloimmune T cell responses induce graft-versus-host disease (GVHD), a serious complication of allogeneic bone marrow transplantation (allo-BMT). Although Notch signaling mediated by Delta-like 1/4 (DLL1/4) Notch ligands has emerged as a major regulator of GVHD pathogenesis, little is known about the timing of essential Notch signals and the cellular source of Notch ligands after allo-BMT. Here, we have shown that critical DLL1/4-mediated Notch signals are delivered to donor T cells during a short 48-hour window after transplantation in a mouse allo-BMT model. Stromal, but not hematopoietic, cells were the essential source of Notch ligands during in vivo priming of alloreactive T cells. GVHD could be prevented by selective inactivation of Dll1 and Dll4 in subsets of fibroblastic stromal cells that were derived from chemokine Ccl19-expressing host cells, including fibroblastic reticular cells and follicular dendritic cells. However, neither T cell recruitment into secondary lymphoid organs nor initial T cell activation was affected by Dll1/4 loss. Thus, we have uncovered a pathogenic function for fibroblastic stromal cells in alloimmune reactivity that can be dissociated from their homeostatic functions. Our results reveal what we believe to be a previously unrecognized Notch-mediated immunopathogenic role for stromal cell niches in secondary lymphoid organs after allo-BMT and define a framework of early cellular and molecular interactions that regulate T cell alloimmunity.
A key-management scheme based on control vectors (1991)

This paper presents a cryptographic key-management scheme based on control vectors. This is a new concept that permits cryptographic keys belonging to a cryptographic system to be easily, securely, and efficiently controlled. The new key-management scheme—built on the cryptographic architecture and key management implemented in a prior set of IBM cryptographic products—has been implemented in the newly announced IBM Transaction Security System.
Dislocation of a posterior stabilized total knee prosthesis. A report of two cases. (1990)

Posterior stabilized knee prostheses have been recommended for knees with posterior cruciate deficiency, as well as for knees with prior patellectomy. Two cases are presented in which a complete dislocation of a Kinematic II Stabilizer prosthesis occurred after primary knee arthroplasty. The mechanism of dislocation was a varus or valgus stress while the knee was flexed. This previously unreported complication of dislocation after primary knee arthroplasty with a posterior stabilized knee prosthesis was, we believe, due, in part, to the design of this prosthesis, which provides little mediolateral stability in flexion, in combination with a mild degree of laxity of the collateral ligaments. This complication could be prevented by use of a prosthesis with greater inherent mediolateral stability.
Nucleophilic adducts of N-tert-butyloxycarbonyl-1,1,1,3,3,3-hexafluoroisopropylimine. Facile hydrolysis of imidazole-based adducts (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleophilic adducts of N-tert-butyloxycarbonyl-1,1,1,3,3,3-hexafluoroisopropylimine. Facile hydrolysis of imidazole-based adductsHerbert H. Seltzman and Toby M. ChapmanCite this: J. Org. Chem. 1975, 40, 16, 2414–2416Publication Date (Print):August 1, 1975Publication History Published online1 May 2002Published inissue 1 August 1975https://pubs.acs.org/doi/10.1021/jo00904a037https://doi.org/10.1021/jo00904a037research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Prediction of Cancer Drug Resistance and Implications for Personalized Medicine (2015)

Drug resistance still impedes successful cancer chemotherapy. A major goal of early concepts in individualized therapy was to develop in vitro tests to predict tumors' drug responsiveness. We have developed an in vitro short-term test based on nucleic acid precursor incorporation to determine clinical drug resistance. This test detects inherent and acquired resistance in vitro and transplantable syngeneic and xenografted tumors in vivo. In several clinical trials, clinical resistance was predictable with more than 90% accuracy, while drug sensitivity was detected with less accuracy (~60%). Remarkably, clinical cross-resistance to numerous drugs (multidrug resistance, broad spectrum resistance) was detectable by a single compound, doxorubicin, due to its multifactorial modes of action. The results of this predictive test were in good agreement with predictive assays of other authors. As no predictive test has been established as yet for clinical diagnostics, the identification of sensitive drugs may not reach sufficiently high reliability for clinical routine. A meta-analysis of the literature published during the past four decades considering test results of more than 15,000 tumor patients unambiguously demonstrated that, in the majority of studies, resistance was correctly predicted with an accuracy between 80 and 100%, while drug sensitivity could only be predicted with an accuracy of 50-80%. This synopsis of the published literature impressively illustrates that prediction of drug resistance could be validated. The determination of drug resistance was reliable independent of tumor type, test assay, and drug used in these in vitro tests. By contrast, chemosensitivity could not be predicted with high reliability. Therefore, we propose a rethinking of the "chemosensitivity" concept. Instead, predictive in vitro tests may reliably identify drug-resistant tumors. The clinical consequence imply to subject resistant tumors not to chemotherapy, but to other new treatment options, such as antibody therapy, adoptive immune therapy, hyperthermia, gene therapy, etc. The high accuracy to predict resistant tumors may be exploited to develop new strategies for individualized cancer therapy. This new concept bears the potential of a revival of predictive tests for personalized medicine.
Quantitative Contrast-Enhanced Magnetic Resonance Lymphangiography of the Upper Limbs in Breast Cancer Related Lymphedema: An Exploratory Study (2015)

This work demonstrated the feasibility of CE-MRL of the upper limbs in patients with BRCL, introducing an advanced imaging and analysis protocol suitable for anatomical and functional study of the lymphatic system.
Measuring the Impact of Tinnitus on Aided Listening Effort Using Pupillary Response (2018)

Tinnitus can have serious impact on a person's life and is a common auditory symptom that is especially comorbid with hearing loss. This study investigated processing effort required for speech recognition in a group of hearing-impaired people with tinnitus and a control group (CG) of hearing-impaired people without tinnitus by means of pupillary response. Furthermore, the relationship between the pupillary response, self-rating measures of tinnitus severity, and fatigue was examined. Participants performed a speech-in-noise task with a competing four-talker babble at two speech intelligibility levels (50% and 95%) with either an active or inactive noise-reduction scheme while the pupillary response was recorded. Tinnitus participants showed significantly smaller time-dependent pupil dilations and significantly higher fatigue ratings. No correlation was found for the tinnitus severity and pupillary response, but a significant correlation was found between the tinnitus severity and fatigue. As participants with tinnitus generally reported higher fatigue and showed smaller task-evoked pupil dilations, it was speculated that this may suggest an increased activity of the parasympathetic nervous system, which governs the bodily response during rest. The finding that tinnitus participants showed higher fatigue has clinical implications, highlighting the importance of taking steps to decrease the risk of developing long-term fatigue. Finally, the tinnitus participants showed reduced pupillary responses when noise reduction was activated, suggesting a reduced effort from hearing aid signal processing.
Fiber-based adsorbents having high adsorption capacities for recovering dissolved metals and methods thereof (2023)

A fiber-based adsorbent and a related method of manufacture are provided. The fiber-based adsorbent includes polymer fibers with grafted side chains and an increased surface area per unit weight over known fibers to increase the adsorption of dissolved metals, for example uranium, from aqueous solutions. The polymer fibers include a circular morphology in some embodiments, having a mean diameter of less than 15 microns, optionally less than about 1 micron. In other embodiments, the polymer fibers include a non-circular morphology, optionally defining multiple gear-shaped, winged-shaped or lobe-shaped projections along the length of the polymer fibers. A method for forming the fiber-based adsorbents includes irradiating high surface area polymer fibers, grafting with polymerizable reactive monomers, reacting the grafted fibers with hydroxylamine, and conditioning with an alkaline solution. High surface area fiber-based adsorbents formed according to the present method demonstrated a significantly improved uranium adsorption capacity per unit weight over existing adsorbents.
Genetic variation of root and shoot traits at early stage in a lentil (Lens culinaris Medik) recombinant inbred lines (RILs) population under drought: towards Quantitative Trait Loci (QTL) mapping (2014)

Well-developed roots and vigorous shoots at early-seedling stage are important for drought tolerance in lentil (Lens culinaris Medik). 135 RILs derived from a cross between two contrasting parents ILL6002 and ILL5888 (obtained from JF Muehlbauer, Washington State University) is under phenotyping and genotyping in order to assess the genetic control behind root and shoot traits at early growth stage. The phenotyping was carried out in a plastic pot experiment under well-watered and progressive drought-stressed treatments. ILL6002 is a vigorous line with a well developed root system showing drought tolerance, while ILL5888 has a less developed root system and vegetative biomass showing drought sensitivity. The analysis of variance shows significant differences between the RILs for all the studied traits. Significant correlations with drought tolerance were observed for dry root weight (0,616), root surface area (0,516), lateral root number (0,476), root-shoot ratio (0,377), chlorophyll content (0,452) and dry shoot weight (0,282). The frequency distributions of dry root weight, lateral root number, taproot length, mean taproot diameter, root surface area, dry shoot weight, shoot length at 12 and 22 days after sowing, seedling vigour, chlorophyll content, root-shoot ratio and drought score in the RILs were quantitative, continuous and normally distributed. Thus, suggesting polygenic control of these traits involving probably many QTLs of variable effects. The existing genetic map is being saturated by Amplified Fragment Length Polymorphism and microsatellite markers in order to enhance the resolution of QTL mapping.
Characteristics of saxitoxin binding to the sodium channel of sarcolemma isolated from rat skeletal muscle. (1979)

1. The characteristics of saxitoxin (STX) binding to the mammalian Na channel have been studied in purified sarcolemma isolated from rat skeletal muscle. 2. STX binds specifically to isolated sarcolemma with a Kd of 1.43 x 10(‐9) M and Bmax of 7‐8 p‐mole STX bound/mg membrane protein at 0 degrees C in the presence of 140 mM‐NaCl. In rat muscle homogenate under the same conditions the corresponding values are Kd = 1.53 x 10(‐9) M and Bmax = 0.15‐0.20 p‐mole/mg protein (18‐20 p‐mole/g wet wt.). Membrane purification produced a fortyfold increase in STX binding site concentration per milligram protein. Calculated binding site density in isolated sarcolemma was about 30 sites/micron 2 of membrane surface. 3. Denervation (10‐14 days) results in a 43% reduction in the density of high‐affinity STX binding sites in purified sarcolemma, but the Kd for this class of sites is not changed. 4. In sarcolemma, the apparent Kd for STX binding is dependent on temperature pH and ionic strength. The Q10 for Kd between 0 and 40 degrees C is 1.3. Protonation of a group having a pK of 6.0 markedly raises Kd without affecting Bmax. Apparent Kd increases eightfold when ionic strength is raised from 20 to 600 mM. 5. Dissociation and association rate constants for STX binding are temperature dependent with Q10 of 2.6 and 1.9 respectively between 0 and 20 degrees C. 6. STX binding is competitively inhibited by monovalent and divalent cations under conditions of constant total ionic strength. An affinity sequence of Tl+ greater than Li+ greater than Na+ greater than K+ greater than Rb+ greater than Cs+ is seen for the monovalent cation‐binding site. 7. The STX binding site is relatively stable to heat and to enzymic degradation. A specific modifier of carboxyl residues inactivates subsequent STX binding. This process can be prevented by the presence of STX during the reaction. 8. Characteristics of the STX binding site in isolated sarcolemma are compared to those reported for other isolated excitable membranes and for studies of whole muscle and muscle homogenate. Sarcolemma provides a potential source of enriched Na channels for further purification efforts in a mammalian system.
Automated Place Classification Using Object Detection (2010)

Places in an environment can be described by the objects they contain. This paper discusses the completely automated integration of object detection and place classification in a single system. We first perform automated learning of object-place relations from an online annotated database. We then train object detectors on some of the most frequently occurring objects. Finally, we use detection scores as well as learned object-place relations to perform place classification of images. We also discuss areas for improvement and the application of this work to informed visual search. As a whole, the system demonstrates the automated acquisition of training data containing labeled instances (i.e. bounding boxes) and the performance of a state-of-the-art object detection technique trained on this data to perform place classification of realistic indoor scenes.