gebhart/patrepeval huggingface dataset:
Comparison of a Respiratory Suspension Aerosolized by an Air-Jet and an Ultrasonic Nebulizer (1996)

In the absence of USP standards and performance monographs, this research sought to determine if differences in the aerosolization mechanism (air-jet vs. ultrasonic) affected droplet and insoluble particle deposition of a nebulized model respiratory suspension. Five milliliters of a model suspension containing 0.1% w/v fluorescein (to estimate droplet deposition) and known quantities of 1, 3, and 6 microns latex spheres (representing insoluble drug particles) was aerosolized from an air-jet and an ultrasonic nebulizer. Nebulized output was collected in a modified Andersen impactor. Samples were analyzed spectrophotometrically (490.5 nm) and by a Coulter Counter to estimate droplet and sphere deposition, respectively. The distribution of droplets throughout the modified impactor for both nebulizers suggested that both the air-jet and the ultrasonic nebulizer produced droplets (0.4 to 10 microns in aerodynamic diameter) large enough to incorporate 1, 3, and 6 microns insoluble spheres. However, Coulter Counter analysis of the sphere distribution revealed that while the air-jet nebulized output contained spheres of all sizes, this was not true for the ultrasonic nebulizer. In the ultrasonic nebulizer, 99% of the spheres (irrespective of size) were not aerosolized and were recovered from the nebulizer reservoir at the aerosolization end point. The results highlight the importance of evaluating performance of a respiratory suspension in combination with a specific nebulizer. When conducting in vitro inertial deposition testing of a respiratory suspension, it is inappropriate to assume that deposition trends of droplets will predict the deposition of the insoluble dispersed phase.
Synthesis of UV‐curable/alkali‐soluble dispersants used for black photoresist with a high loading of carbon black (2009)

Abstract This research synthesized a functional dispersant, not only providing a good dispersion of carbon black (CB) but also possessing ultraviolet (UV)‐curable and alkali‐soluble properties, by a two‐step process. Firstly, bisphenol‐A epoxy diacrylate was reacted with benzophenone tetracarboxylic dianhydride at different molar ratios to obtain UV‐curable/alkali‐soluble resins. In the second step, these resins would possess dispersion ability of CB by reaction with an isocyanate‐containing methacrylate. The prepared dispersants were evaluated by their dispersion ability and the light absorption property of CB. The results showed that one of the dispersants was able to disperse CB in the solvent up to 10 wt % with a mean particle size about 100 nm. This particular dispersant had a moderate amount of amino‐containing groups (such as urethane, amide, and imide) serving as anchoring sites on CB, and a sufficiently long chain ( M n ∼ 2600) to provide a steric repulsion among CB particles. The advantage of this CB/dispersant system is that no other curable resins are needed. By using a suitable photoinitiator, the present CB/dispersant system could reach a polymerization rate of 1.05 × 10 −3 (s –1 ), and a black pattern of 10 μm in width on a glass substrate was obtained through an UV‐lithography process. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Feeding of different levels of metabolite combinations produced byLactobacillus plantarumon growth performance, fecal microflora, volatile fatty acids and villi height in broilers (2010)

The effects of feeding different dosages of metabolite combination of L. plantarum RS5, RI11, RG14 and RG11 strains (Com3456) on the performance of broiler chickens was studied. A total of 504 male Ross broilers were grouped into 7 treatments and offered different diets: (i) standard corn-soybean based diet (negative control); (ii) standard corn-soybean based diet +100 ppm neomycin and oxytetracycline (positive control); (iii) standard corn-soybean based diet + 0.1% metabolite combination of L. plantarum RS5, RI11, RG14 and RG11 strains (Com3456); (iv) standard corn-soybean based diet + 0.2% of Com3456; (v) standard corn-soybean based diet + 0.3% of Com3456 (vi) standard corn-soybean based diet + 0.4% of Com3456 and (vii) standard corn-soybean based diet + 0.5% of Com3456. Supplementation of Com3456 with different dosages improved growth performance, reduced Enterobacteriaceae and increased lactic acid bacteria count, and increased villi height of small intestine and fecal volatile fatty acid concentration. Treatment with 0.4% and 0.2% Com3456 had the best results, especially in terms of growth performance, feed conversion ratio and villi height among other dosages. However, the dosage of 0.2% was recommended due to its lower concentration yielding a similar effect as 0.4% supplementation. These results indicate that 0.2% is an optimum level to be included in the diets of broiler in order to replace antibiotic growth promoters.
The absence of serum IgE antibodies indicates non‐type 2 disease in young asthmatics (2018)

Clinically relevant elevation of type 2 biomarkers was seen in young asthmatics with IgE antibodies <0.35 kU<sub>A</sub> /L, but not those with IgE < 0.10 kU<sub>A</sub> /L. It seems possible to define non-type 2 asthma through sensitive IgE-antibody measurement.
Controlling fluid flow to improve cell seeding uniformity (2018)

Abstract Standard methods for seeding monolayer cell cultures in a multiwell plate or dish do not uniformly distribute cells on the surface. With traditional methods, users find aggregation around the circumference, in the centre, or a combination of the two. This variation is introduced due to the macro scale flow of the cell seeding suspension, and movement of the dish before cells can settle and attach to the surface. Reproducibility between labs, users, and experiments is hampered by this variability in cell seeding. We present a simple method for uniform and user-independent cell seeding using an easily produced uniform cell seeder (UCS) device. This allows precise control of cell density in a reproducible manner. By containing the cell seeding suspension in a defined volume above the culture surface with the UCS, fluctuations in cell density are minimised. Seeding accuracy, as defined by the actual cell density versus the target seeding density is improved dramatically across users with various levels of expertise. We go on to demonstrate the impact of local variation in cell density on the lineage commitment of human embryonic stem cells (hESCs) towards pancreatic endoderm (PE). Variations in the differentiation profile of cells across a culture well closely mirror variations in cell density introduced by seeding method – with the UCS correcting variations in differentiation efficiency. The UCS device provides a simple and reproducible method for uniform seeding across multiple culture systems.
Ataxia-telangiectasia mutated kinase regulates ribonucleotide reductase and mitochondrial homeostasis (2007)

Ataxia-telangiectasia mutated (ATM) kinase orchestrates nuclear DNA damage responses but is proposed to be involved in other important and clinically relevant functions. Here, we provide evidence for what we believe are 2 novel and intertwined roles for ATM: the regulation of ribonucleotide reductase (RR), the rate-limiting enzyme in the de novo synthesis of deoxyribonucleoside triphosphates, and control of mitochondrial homeostasis. Ataxia-telangiectasia (A-T) patient fibroblasts, wild-type fibroblasts treated with the ATM inhibitor KU-55933, and cells in which RR is inhibited pharmacologically or by RNA interference (RNAi) each lead to mitochondrial DNA (mtDNA) depletion under normal growth conditions. Disruption of ATM signaling in primary A-T fibroblasts also leads to global dysregulation of the R1, R2, and p53R2 subunits of RR, abrogation of RR-dependent upregulation of mtDNA in response to ionizing radiation, high mitochondrial transcription factor A (mtTFA)/mtDNA ratios, and increased resistance to inhibitors of mitochondrial respiration and translation. Finally, there are reduced expression of the R1 subunit of RR and tissue-specific alterations of mtDNA copy number in ATM null mouse tissues, the latter being recapitulated in tissues from human A-T patients. Based on these results, we propose that disruption of RR and mitochondrial homeostasis contributes to the complex pathology of A-T and that RR genes are candidate disease loci in mtDNA-depletion syndromes.
Full process control strategy of fuel based on water-coal ratio of ultra supercritical units (2011)

In order to realize the full process control of fuel in ultra supercritical units, fuel control strategy of feeders in start-up stage to guarantee the stability of coal-burning is proposed firstly. The control strategy of coal feeder which can make the bias trend to zero automatically is discussed. Control strategy of base amount of fuel is presented. The base amount of fuel in start-up phase is determined by states of units: cold state, warm state, hot state and extreme hot state. Water-coal ratio controls separator inlet temperature, outlet flue temperature, main steam pressure or separator inlet superheat in different start-up stages. The control strategy has been successfully applied to 1036MW ultra-supercritical units. It is a valuable reference for the design of similar control system.
A Requirement for p120-catenin in the metastasis of invasive ductal breast cancer (2020)

We report here the effects of targeted p120-catenin (encoded by <i>CTNND1</i>; hereafter denoted p120) knockout (KO) in a PyMT mouse model of invasive ductal (mammary) cancer (IDC). Mosaic p120 ablation had little effect on primary tumor growth but caused significant pro-metastatic alterations in the tumor microenvironment, ultimately leading to a marked increase in the number and size of pulmonary metastases. Surprisingly, although early effects of p120-ablation included decreased cell-cell adhesion and increased invasiveness, cells lacking p120 were almost entirely unable to colonized distant metastatic sites <i>in vivo</i> The relevance of this observation to human IDC was established by analysis of a large clinical dataset of 1126 IDCs. As reported by others, p120 downregulation in primary IDC predicted worse overall survival. However, as in the mice, distant metastases were almost invariably p120 positive, even in matched cases where the primary tumors were p120 negative. Collectively, our results demonstrate a strong positive role for p120 (and presumably E-cadherin) during metastatic colonization of distant sites. On the other hand, downregulation of p120 in the primary tumor enhanced metastatic dissemination indirectly via pro-metastatic conditioning of the tumor microenvironment.
Comparison of clinical outcomes between HIV-infected patients with and without HCV co-infection in a resource-limited setting. (2012)

Hepatitis C virus (HCV) co-infection is common among HIV-infected patients; its treatment is not affordable in resource-limited settings. This study aimed to compare the morbidity, mortality, immunological and virological outcomes of antiretroviral therapy (ART) between HIV-infected patients with and without HCV co-infection in a setting where HCV infection is rarely treated. A retrospective cohort study was conducted among HIV-infected patients attending Ramathibodi Hospital between 1998 and 2008. We studied 171 HIV-infected patients 57 with and 114 without HCV co-infection. The mean age of patients was 34.6 years and 67.3% were males. There were no differences in demographics, HIV staging, CD4 counts, ART use and ART regimens between the two groups (p>0.05). All patients who had a CD4 count <200 cells/mm3 or had an AIDS-defining illness during following-up were given ART; these consisted of 84.2% and 88.6% of patients with and without HCV co-infection, respectively. Only 4 out of 57 (7%) HCV co-infected patients were treated for HCV infection. During a median (range) follow-up time of 2.9 (1.2-9.8) years, no patients died in either group. The rates of AIDS-defining illnesses and hospitalization in the two groups were similar (p>0.05). In a resource-limited setting where HCV treatment is not affordable, HCV co-infection does not appear to affect morbidity, mortality or treatment responses to ART. ART may have a greater impact than HCV co-infection on the survival of HCV/HIV co-infected patients. Further studies are needed to assess the long-term impact of HCV co-infection on clinical outcomes in HIV-infected patients without HCV treatment.
Theoretical and methodological pathways for research on elites (2018)

In this introductory essay to our special issue on elites, we outline some of the major challenges to research in this area and propose a series of theoretical and methodological pathways to address them. Theoretically we make four recommendations: (a) greater attentiveness to and specificity about the relationship between elites and power; (b) a clearer articulation of the relationships between elites and the varieties of capitalism; (c) far more attention to diversity within elites and the use of elites to understand forms of domination like white supremacy and masculine domination and (d) expanding beyond the orthodox form of Bourdieusian theoretical frameworks. Methodologically we outline how research using survey instruments, social network analysis (SNA) (and multiple correspondence analysis), interviews, ethnographic observation, experiments, archival research, administrative data and content analysis can each be deployed, built upon or redirected to help bring elites into greater focus.
T cell recognition of fibrinogen. A determinant on the A alpha-chain does not require processing. (1988)

Abstract Murine T cell hybridomas were used to examine the requirements for processing and presentation of human fibrinogen. In contrast to most protein Ag, fibrinogen (Mr 340,000) did not need to be processed by an APC, inasmuch as intact fibrinogen was presented by both pre-fixed and chloroquine-treated macrophages. Through the use of a variety of protease inhibitors, no involvement of proteases either on the macrophage surface or in the culture medium in the presentation of fibrinogen was observed. The epitope recognized by two T cell hybridomas was localized to the peptide, A alpha (551-578), which was located on the carboxy portion of the A alpha-chain. This portion of the A alpha-chain has no defined secondary structure and must possess the conformational flexibility which allows it to directly associate with an I-Ek molecule. Thus conformational flexibility may be a major factor in determining the processing requirements of a protein Ag.
An analysis of a gyro-free inertial system for INS/GNSS navigation (2016)

Although the concept of determining angular velocity out of the readings of multiple, displaced accelerometers is not new, apparently it has not yet matured into practical technologies. A new generation of low-cost MEMS accelerometers, which is expected to breakthrough in the next years, may enable gyro-free navigation in small platforms such as UAVs, ground vehicles and small planes. In this work we analyze a Gyro-Free Inertial Navigation System (GF-INS) employing four triads of accelerometers in a Distributed Redundant IMU configuration (DRIMU). We investigate the statistical properties of the angular velocity estimator by means of simulations and we speculate on the noise level requirements for the accelerometers that would allow a gyro-free navigation system to match the performances of a conventional IMU. Furthermore, we investigate the performance of using the gyro-free angular velocities estimator in combination with GNSS for navigation in a classical error-state EKF.
Real-Time Measurement of End-to-End Available Bandwidth using Kalman Filtering (2006)

This paper presents a new method, BART (bandwidth available in real-time), for estimating the end-to-end available bandwidth over a network path. It estimates bandwidth quasi-continuously, in real-time. The method has also been implemented as a tool. It relies on self-induced congestion, and repeatedly samples the available bandwidth of the network path with sequences of probe packet pairs, sent at randomized rates. BART requires little computation in each iteration, is lightweight with respect to memory requirements, and adds only a small amount of probe traffic. The BART method uses Kalman filtering, which enables real-time estimation (a.k.a. tracking). It maintains a current estimate, which is incrementally improved with each new measurement of the inter-packet time separations in a sequence of probe packet pairs. The measurement model has a strong non-linearity, and would not at first sight be considered suitable for Kalman filtering, but we show how this non-linearity can be handled. BART may be tuned according to the specific needs of the measurement application, such as agility vs. stability of the estimate. We have tested an implementation of BART in a physical test network with carefully controlled cross traffic, with good accuracy and agreement. Test measurements have also been performed over the Internet. We compare the performance of BART with that of pathChirp, a state-of-the-art tool for measuring end-to-end available bandwidth in real-time
Comparative Sequence Analysis of the Sorghum RphRegion and the Maize Rp1 Resistance Gene Complex (2002)

A 268-kb chromosomal segment containing sorghum (Sorghum bicolor) genes that are orthologous to the maize (Zea mays) Rp1 disease resistance (R) gene complex was sequenced. A region of approximately 27 kb in sorghum was found to contain five Rp1 homologs, but most have structures indicating that they are not functional. In contrast, maize inbred B73 has 15 Rp1 homologs in two nearby clusters of 250 and 300 kb. As at maize Rp1, the cluster of R gene homologs is interrupted by the presence of several genes that appear to have no resistance role, but these genes were different from the ones found within the maize Rp1 complex. More than 200 kb of DNA downstream from the sorghum Rp1-orthologous R gene cluster was sequenced and found to contain many duplicated and/or truncated genes. None of the duplications currently exist as simple tandem events, suggesting that numerous rearrangements were required to generate the current genomic structure. Four truncated genes were observed, including one gene that appears to have both 5' and 3' deletions. The maize Rp1 region is also unusually enriched in truncated genes. Hence, the orthologous maize and sorghum regions share numerous structural features, but all involve events that occurred independently in each species. The data suggest that complex R gene clusters are unusually prone to frequent internal and adjacent chromosomal rearrangements of several types.
Inside Front Cover: Polyvinylpyrrolidone‐Directed Crystallization of ZnO with Tunable Morphology and Bandgap (Adv. Funct. Mater. 18/2007) (2007)

Abstract A biomimetic approach for the shape‐selective synthesis of ZnO particles with controlled band gaps and morphologies at low temperatures is reported by Jianhui Zhang and co‐workers on p. 3897. Shape‐selective synthesis of ZnO is achieved by selective passivation of specific ZnO facets. Up to fifteen types of high‐purity ZnO structures were produced in this manner, allowing adjustment of room‐temperature photoluminescence and band gap. A novel polyvinylpyrrolidone (PVP)‐directed crystallization route is successfully developed for the shape‐selective synthesis of ZnO particles with distinctive shapes, including monolayer, bilayer, and multilayer structures, gears, capped pots, hemispheres, and bowls, at temperatures as low as 32 °C. This route is based on exploiting a new water/PVP/ n ‐pentanol system. In the system, PVP can greatly promote ZnO nucleation by binding water and direct ZnO growth by selectively capping the specific ZnO facets, which is confirmed by IR absorption spectra. The bandgap of the ZnO particles is readily tuned by modifying the product morphology by adjusting the PVP chain length, PVP amount, water volume, and reaction temperature. The remarkable ZnO structures and the biomimetic method demonstrated here not only expand the structures and applications of ZnO but also provide a new approach to explore the unusual structures for novel physicochemical properties and technological applications. Furthermore, the novel ZnO/Au/ZnO sandwich structure is successfully fabricated by inserting a Au plate into the bilayer ZnO structure.
Crystal orientation of GaN layers on (1010) m‐plane sapphire (2011)

Abstract Based on the atomic arrangement of the $(10{\bar {1}}0)$ m ‐plane sapphire surface, we have developed a model for the initial nucleation process of gallium‐nitride (GaN). This model describes why $(11{\bar {2}}2)$ and $(10{\bar {1}}{\bar {3}})$ are the preferred orientations of GaN on the $(10{\bar {1}}0)$ sapphire. The experimental results from high‐resolution X‐ray diffraction measurements, like the crystallographic relations and the twinning of the $(1{\bar {1}}0{\bar {3}})$ orientation are explained by the model too. Our model also predicts that $(11{\bar {2}}2)$ thin films are metal‐polar and $(1{\bar {1}}0{\bar {3}})$ thin films are nitrogen‐polar.
CFD-Guided Heavy Duty Mixing-Controlled Combustion System Optimization with a Gasoline-Like Fuel (2017)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;A computational fluid dynamics (CFD) guided combustion system optimization was conducted for a heavy-duty compression-ignition engine with a gasoline-like fuel that has an anti-knock index (AKI) of 58. The primary goal was to design an optimized combustion system utilizing the high volatility and low sooting tendency of the fuel for improved fuel efficiency with minimal hardware modifications to the engine. The CFD model predictions were first validated against experimental results generated using the stock engine hardware. A comprehensive design of experiments (DoE) study was performed at different operating conditions on a world-leading supercomputer, MIRA at Argonne National Laboratory, to accelerate the development of an optimized fuel-efficiency focused design while maintaining the engine-out NO&lt;sub&gt;x&lt;/sub&gt; and soot emissions levels of the baseline production engine. Compared to the base engine, the optimized results showed a significant improvement in closed-cycle, indicated specific fuel consumption (ISFC) across different engine speed and load points. When combined with modified injector configurations, the optimized piston bowl designs showed better in-cylinder air utilization and shorter combustion duration, thereby leading to improved fuel efficiency of up to 2.8%. In particular, increasing the injector hydraulic flow rate (larger nozzle diameter) was found to be beneficial by shortening the combustion duration while producing a higher incylinder combustion temperature for enhanced soot oxidation. A lower swirl ratio was also seen to be beneficial and the effects were attributed to the lower heat transfer loss and reduced need for fuel-air mixing. Increasing the compression ratio from 18.9 to 20.5 was also important for improving the fuel efficiency according to the relative contributions from key design parameters on ISFC.&lt;/div&gt;&lt;/div&gt;
Progressive coding of palette images and digital maps (2003)

A 2D version of PPM (Prediction by Partial Matching) coding is introduced simply by combining a 2D template with the standard PPM coding scheme. A simple scheme for resolution reduction is given and the 2D PPM scheme extended to resolution progressive coding by placing pixels in a lower resolution image layer. The resolution is increased by a factor of 2 in each step. The 2D PPM coding is applied to palette images and street maps. The sequential results are comparable to PWC. The PPM results are a little better for the palette images with few colors (up to 4-5 bpp) and a little worse for the images with more colors. For street maps the 2D PPM is slightly better. The PPM based resolution progressive coding provides a better result than coding the resolution layers as individual images. Compared to GIF the resolution progressive 2D PPM's coding efficiency is significantly better. An example of combined content-layer/spatial progressive coding is also given.