gebhart/patrepeval huggingface dataset:
Visualization of Cell Surface Vasopressin V1a Receptors in Rat Hepatocytes with a Fluorescent Linear Antagonist (1999)

To visualize cell surface V1a vasopressin receptors in rat hepatocytes in the absence of receptor-mediated endocytosis, we used a high-affinity fluorescent linear antagonist, Rhm8-PVA. Epifluorescence microscopy (3CCD camera) and fluorescence spectroscopy were used. Rhm8-PVA alone did not stimulate Ca2+ signals and competitively blocked Ca2+ signals (Kinact of 3.0 nM) evoked by arginine vasopressin (vasopressin). When rat hepatocytes were incubated with 10 nM of Rhm8-PVA for 30 min at 4C, the fluorescent antagonist bound to the surface of cells, presumably the plasma membrane. The V1a receptor specificity of Rhm8-PVA binding was confirmed by its displacement by the nonfluorescent antagonist V4253 and by the natural hormone vasopressin at 4C. Prior vasopressin-mediated endocytosis of V1a receptors at 37C abolished binding of the labeled antagonist, whereas in non-preincubated cells, Rhm8-PVA labeled the cell surface of rat hepatocytes. When cells labeled with Rhm8-PVA at 4C were warmed to 37C to initiate receptor-mediated internalization of the fluorescent complex, Rhm8-PVA remained at the cell surface. Incubation temperature at 4C or 37C had little effect on binding of Rhm8-PVA. We conclude that Rhm8-PVA is unable to evoke receptor-mediated endocytosis and can readily be used to visualize cell surface receptors in living cells.
MR-X1 &#x2014; An AUV equipped with a space distributed CPU system and a satellite telecontrol interface (2009)

JAMSTEC has planned development of a long-rang cruising AUV. This vehicle will enable over 20 days autonomous cruising. To achieve this vehicle, we need various basic technology developments. In this article, a space distributed CPU system development for achieving highly redundant system control and a stationary satellite control system for remote control of the vehicle are described. We carried out sea trial to make sure the each system function using a test bed vehicle. It was shown that the satellite communication system is more effective.
Possibility to Use Product Image and Review Text Based on the Association between Onomatopoeia and Texture (2015)

With the widespread use of online shopping in recent years, consumer search requests for products have become more diverse. Previous web search methods have used adjectives as input by consumers. However, given that the number of adjectives that can be used to express textures is limited, it is debatable whether adjectives are capable of richly expressing variations of product textures. In Japanese, tactile and visual textures are easily and frequently expressed by onomatopoeia, such as ``fuwa-fuwa'' for a soft and light sensation and ``kira-kira'' for a glossy texture. Onomatopoeia are useful for understanding not only material textures but also a user's intuitive, sensitive, and even ambiguous feelings evoked by materials. In this study, we propose a system to rank FMD images corresponding to texture associated with Japanese onomatopoeia based on their symbolic sound associations between the onomatopoeia phonemes and the texture sensations. Our system quantitatively estimates the texture sensations of input onomatopoeia, and calculates the similarities between the users' impressions of the onomatopoeia and those of the images. Our system also suggests the images which best match the input onomatopoeia. An evaluation of our method revealed that the best performance was achieved when the SIFT features, the colors of the images, and text describing impressions of the images were used.
Latrepirdine: molecular mechanisms underlying potential therapeutic roles in Alzheimer’s and other neurodegenerative diseases (2013)

Latrepirdine (Dimebon(TM)) was originally marketed as a non-selective antihistamine in Russia. It was repurposed as an effective treatment for patients suffering from Alzheimer's disease (AD) and Huntington's disease (HD) following preliminary reports showing its neuroprotective functions and ability to enhance cognition in AD and HD models. However, latrepirdine failed to show efficacy in phase III trials in AD and HD patients following encouraging phase II trials. The failure of latrepirdine in the clinical trials has highlighted the importance of understanding the precise mechanism underlying its cognitive benefits in neurodegenerative diseases before clinical evaluation. Latrepirdine has shown to affect a number of cellular functions including multireceptor activity, mitochondrial function, calcium influx and intracellular catabolic pathways; however, it is unclear how these properties contribute to its clinical benefits. Here, we review the studies investigating latrepirdine in cellular and animal models to provide a complete evaluation of its mechanisms of action in the central nervous system. In addition, we review recent studies that demonstrate neuroprotective functions for latrepirdine-related class of molecules including the β-carbolines and aminopropyl carbazoles in AD, Parkinson's disease and amyotrophic lateral sclerosis models. Assessment of their neuroprotective effects and underlying biological functions presents obvious value for developing structural analogues of latrepirdine for dementia treatment.
Simultaneous Design by Means of Evolutionary Computation (2012)

This chapter contains sections titled: Simultaneous design of energy systems Evolutionary algorithms and artificial evolution Consideration of multiple objectives Consideration of design constraints Integration of robustness into the simultaneous design process Example applications Conclusions Bibliography
Extremely low-frequency electromagnetic field promotes astrocytic differentiation of human bone marrow mesenchymal stem cells by modulating SIRT1 expression (2017)

It has been shown that extremely low-frequency electromagnetic fields (ELFMF) affect regulation of cell fate and differentiation. Thus, the aim of this study was to investigate the role of ELFMFs in the enhancement of astrocytic differentiation. ELFMF exposure reduced the rate of proliferation and enhanced astrocytic differentiation. The ELFMF-treated cells showed increased levels of the astrocyte marker (GFAP), while those of the early neuronal marker (Nestin) and stemness marker (OCT3/4) were downregulated. The reactive oxygen species (ROS) level was observed to be significantly elevated after ELFMF exposure, which strengthens the modulatory role of SIRT1 and SIRT1 downstream molecules (TLE1, HES1, and MASH1) during astrocytic differentiation. After nicotinamide (5 mM) mediated inhibition of SIRT1, levels of TLE1, HES1, and MASH1 were examined; TLE1 was significantly upregulated and MASH1 was downregulated. These results suggest that ELFMFs induce astrocytic differentiation through activation of SIRT1 and SIRT1 downstream molecules.
The Significance of Preserving the Energy Status and Microcirculation in Liver Grafts from Non-Heart-Beating Donor (2008)

To complete a successful liver transplantation (LTx) from non-heart-beating donors (NHBD), it is necessary to both improve the energy status in liver grafts and to reduce the exposure to free radicals. This study investigated the effects of short perfusion with oxygenated buffer on the grafts prior to cold preservation. In addition, the effects of the antioxidant, biliverdin, for reduction of free radicals was investigated. Male Wistar rats were used. Livers were retrieved, preserved in UW solution, and perfused for 60 min with oxygenated Krebs-Henseleit solution. Rats were allocated to six groups as follows (n=5): (i) control group-no warm ischemia (WI) and cold preservation, (ii) HBD group--no WI with cold preservation for 6 h; (iii) NHBD group--with 30 min of WI and cold preservation, (iv) NM group--with WI including nafamostat mesilate infusion before cardiac arrest and cold preservation; (v) PRE group--with WI, 30-min pre-cold preservation perfusion with oxygenated buffer after cardiac arrest, and cold preservation, (vi) BV group-with the same treatment as the PRE group plus the addition of biliverdin to the pre-cold preservation perfusion. The portal flow volume, bile production, AST, and TNF-alpha in perfusate, energy charge (EC), and ATP level in the tissue, and histological findings were investigated. The portal flow volume in the NM, PRE, and BV groups were higher than in the NHBD group. The bile production in the PRE and BV groups were also higher than in the NHBD group. The EC and ATP level of the BV group after reperfusion were higher than those of the NHBD group. Pre-cold preservation perfusion and addition of biliverdin to perfusate improved viability of grafts from NHBD. The results indicate that the preservation of the energy status and microcirculation of the graft is important for successful LTx from NHBD.
Effects of Teriparatide Compared with Risedronate on Recovery After Pertrochanteric Hip Fracture (2016)

Therapeutic Level II. See Instructions for Authors for a complete description of levels of evidence.
Supplemental Table 1 from The Expression of Four Genes as a Prognostic Classifier for Stage I Lung Adenocarcinoma in 12 Independent Cohorts (2023)

&lt;p&gt;Supplemental Table 1. Datasets considered for inclusion in the meta-analysis and the rationale for inclusion or exclusion.&lt;/p&gt;
Long-Term Permeator Experiment PETRA at the Tritium Laboratory Karlsruhe: Commissioning Tests with Tritium (1995)

ABSTRACTDuring commissioning of the PETRA facility all components were tested singly and sequentially using hydrogen isotopes (incl. up to 1.3 g tritium as DT) and relevant impurities. The operation of the facility in conjunction with the required infrastructure systems of the Tritium Laboratory Karlsruhe (TLK) was also demonstrated.To characterize the PETRA PdAg permeator hydrogen break-through curves for H2, De2 and DT as well as He break-through curves for various H2/D2/He gas mixtures were determined at 300 and 400 °C. A suitable method was developed to verify the mechanical integrity of the permeator during runs with tritium. The H2 and D2 permeation losses into the isolation vacuum of the permeator were quantified and compared with model calculations. Hydrogen permeation into the isolation vacuum could be kept at levels low enough to permit an undisturbed continuous operation of the permeator using a ZrCo tritium storage vessel.All pumps and pump combinations were examined with respect to the achievable vacua and compression ratios employing relevant gases and their mixtures.Loop-integrated infrared analysis of high signal and background stability is used to verify the integrity of the permeator and to study the possible occurrence of radiochemical reactions in the gas phase.It was shown that the ZrCo tritium storage vessel of the PETRA facility can be employed avantageously for the handling of tritium when used in combination with a Normetex scroll pump (18 m3/h)/Siemens metal bellows double stage compressor pump sequence. With this combination it is possible to extract at < 320 °C > 98 % of the hydrogen isotopes from the ZrCo storage vessel with a) negligible permeation losses, b) without the danger of disproportionation of the intermetallic compound and c) with minimization of the tritium inventory in the facility.
Superconductivity above 90 K in the compoundYBa2Cu3Ox: Structural, transport, and magnetic properties (1987)

We report the structural, transport, and magnetic properties of the principal phase responsible for superconductivity in the recently discovered Y-Ba-Cu-O compounds with transition temperatures greater than 90 K.
Is quantum capacitance in graphene a potential hurdle for device scaling? (2014)

Transistor size is constantly being reduced to improve performance as well as power consumption. For the channel length to be reduced, the corresponding gate dielectric thickness should also be reduced. Unfortunately, graphene devices are more complicated due to an extra capacitance called quantum capacitance (C Q) which limits the effective gate dielectric reduction. In this work, we analyzed the effect of C Q on device-scaling issues by extracting it from scaling of the channel length of devices. In contrast to previous reports for metal-insulator-metal structures, a practical device structure was used in conjunction with direct radio-frequency field-effect transistor measurements to describe the graphene channels. In order to precisely extract device parameters, we reassessed the equivalent circuit, and concluded that the on-state model should in fact be used. By careful consideration of the underlap region, our device modeling was shown to be in good agreement with the experimental data. C Q contributions to equivalent oxide thickness were analyzed in detail for varying impurity concentrations in graphene. Finally, we were able to demonstrate that despite contributions from C Q, grapheme’s high mobility and low-voltage operation allows for graphene channels suitable for next generation transistors.
Fabrication And Characterization Of Poly-Si Vertical Nanowire Thin Film Transistor (2011)

In this paper, we present a vertical nanowire thin film transistor with gate-all-around architecture, fabricated using CMOS compatible processes. A novel method of fabricating polysilicon vertical nanowires of diameter as small as 30 nm using wet-etch is presented. Both n-type and p-type vertical poly-silicon nanowire transistors exhibit superior electrical characteristics as compared to planar devices. On a poly-crystalline nanowire of 30 nm diameter, high Ion/Ioff ratio of 106, low drain-induced barrier lowering (DIBL) of 50 mV/V, and low sub-threshold slope SS~100mV/dec are demonstrated for a device with channel length of 100 nm.
Proliferation and osteogenic differentiation of human bone marrow stromal cells on alginate-gelatine-hydroxyapatite scaffolds with anisotropic pore structure (2008)

Porous mineralized scaffolds are required for various applications in bone engineering. In particular, tube-like pores with controlled orientation inside the scaffold may support homogeneous cell seeding as well as sufficient nutrient supply and may facilitate blood vessel ingrowth. Scaffolds with parallely orientated tube-like pores were generated by diffusion-controlled ionotropic gelation of alginate. Incorporation of hydroxyapatite (HA) during the gelation process yielded stable scaffolds with an average pore diameter of approximately 90 microm. To evaluate the potential use of alginate-gelatine-HA scaffolds for bone tissue engineering, in vitro tests with human bone marrow stromal cells (hBMSCs) were carried out. We analysed biocompatibility and cell penetration into the capillary pores by microscopic methods. hBMSCs were also cultivated on alginate-gelatine-HA scaffolds for 3 weeks in the presence and absence of osteogenic supplements. We studied proliferation and osteogenic differentiation in terms of total lactate dehydrogenase (LDH) activity, DNA content and alkaline phosphatase (ALP) activity and found a 10-14-fold increase of cell number after 2 weeks of cultivation, as well as an increase of specific ALP activity for osteogenic-induced hBMSCs. Furthermore, the expression of bone-related genes [ALP, bone sialoprotein II (BSPII)] was analysed. We found an increase of ALP as well as BSPII expression for osteogenic-induced hBMSCs on alginate-gelatin-HA scaffolds.
Enhancement of the nuclear reactivity of alkylating agents by prednisolone. (1979)

We have compared the nuclear reactions of the alkylating agents chlorambucil, melphalan, and cyclophosphamide used alone, with those produced when the same drugs were used in combination with the corticosteroid prednisolone. Studies were performed in vivo in Wistar rats bearing ascites forms of either the Yoshida sarcoma or the Walker carcinosarcoma. Strains of both tumor lines, which were sensitive to alkylating agents, showed loss of condensed chromatin, increased phosphorylation of nuclear proteins, and increased DNA cross-linking after exposure to the alkylating agents alone; these changes were generally enhanced in the presence of prednisolone. When paired tumor lines which exhibited acquired resistance to alkylating agents were studied, no nuclear changes were detected when individual alkylating agents were given alone. However, in combination with prednisolone, each of the three alkylating agents produced loss of condensed chromatin, increased nuclear protein phosphorylation, and DNA cross-linking. These chromatin changes were associated with tumor cell death and may determine drug sensitivity in the tumors studied.
Fertility Assessment in Sorraia Stallions by Sperm‐Fish and Fkbp6 Genotyping (2016)

The Sorraia, a critically endangered indigenous Iberian horse breed, is characterized by low genetic variability, high rate of inbreeding, bad sperm quality and subfertility. Here, we studied 11 phenotypically normal but subfertile Sorraia stallions by karyotyping, sex chromosome sperm-FISH and molecular analysis of FKBP6 - a susceptibility locus for impaired acrosome reaction (IAR). The stallions had normal sperm concentration (>300 million cells/ml), but the numbers of progressively motile sperm (21%) and morphologically normal sperm (28%) were invariably low. All stallions had a normal 64,XY karyotype. The majority of sperm (89%) had normal haploid sex chromosome content, although 11% of sperm carried various sex chromosome aneuploidies. No correlation was found between the percentage of sperm sex chromosome abnormalities and inbreeding, sperm morphology or stallion age. Direct sequencing of FKBP6 exon 4 for SNPs g.11040315G>A and g.11040379C>A revealed that none of the stallions had the susceptibility genotype (A/A-A/A) for IAR. Instead, all animals had a G/G-A/A genotype - a testimony of low genetic variability. The findings ruled out chromosomal abnormalities and genetic predisposition for IAR as contributing factors for subfertility. However, low fertility of the Sorraia stallions could be partly attributed to relatively higher rate of sex chromosome aneuploidies in the sperm.
T-Cell Receptor Vα Spectratype Analysis of a CD4-Mediated T-Cell Response against Minor Histocompatibility Antigens Involved in Severe Graft-versus-Host Disease (2006)

Although CD4(+) T cells can have an important role in mediating lethal graft-versus-host disease (GVHD) directed to multiple minor histocompatibility antigens (miHA) after bone marrow transplantation, their precise characterization and effector function remains elusive. In this regard, T cell receptor (TCR) Vbeta spectratype analysis has been a powerful tool for identifying donor CD4(+) T cell populations expanding to host miHA after bone marrow transplantation in the major histocompatibility complex-matched C57BL/6 (B6) --> C.B10-H2(b) (BALB.B) model of lethal GVHD. Removal of all of the Vbeta(+) T cell families containing these responding cells from the donor inoculum has proven to be an effective means of preventing the development of GVHD. Previous studies have also found that of the 11 miHA-responsive B6 CD4(+) Vbeta(+) T cell families, transplantation of Vbeta2(+) and Vbeta11(+) T cells together into lethally irradiated BALB.B mice appeared to be primarily responsible for the severity of resultant GVHD. Further focusing on these critical CD4 responses, in this study we demonstrate that B6 CD4(+)Vbeta11(+) T cells alone can induce lethal GVHD in BALB.B recipients. In addition, immunohistochemical staining of host lingual and intestinal epithelial tissues supported the capacity of Vbeta11(+) T cells to infiltrate typical GVHD-associated target areas. To further characterize the specific CD4(+)Vbeta11(+) T cells involved in this anti-miHA response, TCR Valpha spectratype analysis was performed and indicated that 6 Valpha chains were used by this reactive population. These results provide further evidence that a restricted repertoire of T cell specificities, presumably recognizing a correspondingly low number of miHA, is sufficient for the induction of severe GVHD.
Cluster reserves (2000)

In network (e.g., Web) servers, it is often desirable to isolate the performance of different classes of requests from each other. That is, one seeks to achieve that a certain minimal proportion of server resources are available for a class of requests, independent of the load imposed by other requests. Recent work demonstrates how to achieve this performance isolation in servers consisting of a single, centralized node; however, achieving performance isolation in a distributed, cluster based server remains a problem. This paper introduces a new abstraction, the cluster reserve, which represents a resource principal in a cluster based network server. We present a design and evaluate a prototype implementation that extends existing techniques for performance isolation on a single node server to cluster based servers. In our design, the dynamic cluster-wide resource management problem is formulated as a constrained optimization problem, with the resource allocations on individual machines as independent variables, and the desired cluster-wide resource allocations as constraints. Periodically collected resource usages serve as further inputs to the problem. Experimental results show that cluster reserves are effective in providing performance isolation in cluster based servers. We demonstrate that, in a number of different scenarios, cluster reserves are effective in ensuring performance isolation while enabling high utilization of the server resources.
Isolation and characterization of lymphocystis virus (FLDV) from gilt-head sea bream (Sparus aurata, L.) using a new homologous cell line (1999)

Five strains of lymphocystis virus (FLDV) isolated from gilt-head sea bream cultured in several fish farms of Andalusia (South of Spain) were studied. The culture characteristics of all viruses were compared using a new homologous cell line from Sparus aurata. In addition, the serological properties of the viral isolates were also determined comparatively by means of the indirect fluorescent antibody test (IFAT).
Lean Limit A/F Control System by Using Engine Speed Variation (1986)

Systeme de regulation du rapport limite air-carburant (melange pauvre) base sur les variations de la vitesse du moteur
Effect of Smoking on the Pharmacokinetics of Inhaled Loxapine (2014)

Sedation profiles and visual analog scale scores at each time point were similar for nonsmokers and smokers. It was concluded that inhaled loxapine does not require dosage adjustment based on smoking behavior.
Impact of gravity on the bubble‐to‐pulse transition in packed beds (2013)

A model based on two‐phase volume‐averaged equations of motion is proposed to examine the gravity dependence of the bubble‐to‐pulse transition in gas‐liquid cocurrent down‐flow through packed beds. As input, the model uses experimental correlations for the frictional pressure drop under both normal gravity conditions and in the limit of vanishing gravity, as well as correlations for the liquid‐gas interfacial area per unit volume of bed in normal gravity. In accordance with experimental observations, the model shows that, for a given liquid flow, the transition to the pulse regime occurs at lower gas‐flow rates as the gravity level or the Bond number is decreased. Predicted transition boundaries agree reasonably well with observations under both reduced and normal gravity. The model also predicts a decrease in frictional pressure drop and an increase in total liquid holdup with decreasing gravity levels. © 2013 American Institute of Chemical Engineers AIChE J 60: 778–793, 2014