gebhart/patrepeval huggingface dataset:
Plant-derived virus-like particles as vaccines (2012)

Virus-like particles (VLPs) are self-assembled structures derived from viral antigens that mimic the native architecture of viruses but lack the viral genome. VLPs have emerged as a premier vaccine platform due to their advantages in safety, immunogenicity, and manufacturing. The particulate nature and high-density presentation of viral structure proteins on their surface also render VLPs as attractive carriers for displaying foreign epitopes. Consequently, several VLP-based vaccines have been licensed for human use and achieved significant clinical and economical success. The major challenge, however, is to develop novel production platforms that can deliver VLP-based vaccines while significantly reducing production times and costs. Therefore, this review focuses on the essential role of plants as a novel, speedy and economical production platform for VLP-based vaccines. The advantages of plant expression systems are discussed in light of their distinctive posttranslational modifications, cost-effectiveness, production speed, and scalability. Recent achievements in the expression and assembly of VLPs and their chimeric derivatives in plant systems as well as their immunogenicity in animal models are presented. Results of human clinical trials demonstrating the safety and efficacy of plant-derived VLPs are also detailed. Moreover, the promising implications of the recent creation of "humanized" glycosylation plant lines as well as the very recent approval of the first plant-made biologics by the U. S. Food and Drug Administration (FDA) for plant production and commercialization of VLP-based vaccines are discussed. It is speculated that the combined potential of plant expression systems and VLP technology will lead to the emergence of successful vaccines and novel applications of VLPs in the near future.
A Phase I Study of the Hsp90 Inhibitor AUY922 plus Capecitabine for the Treatment of Patients with Advanced Solid Tumors (2015)

AUY922 plus capecitabine was well-tolerated up to 70 mg/m(2) with encouraging preliminary efficacy.
Síntese de novas amidas graxas a partir da aminólise de ésteres metílicos (2010)

<abstract language="eng">Recent biochemical and pharmacological studies have led to the characterization of different fatty acid amides as a new family of biologically active lipids. Here, we describe the synthesis of new amides from C16:0, 18:0, 18:1 and 18:1, OH fatty acids (FFA) families with cyclic and acyclic amines and demonstrate for the first time that these compounds produce citotoxic effects. Application of this method to the synthesis of fatty acid amides was performed using the esters aminolysis as a key step and various carboxylic amides were prepared in good yield from fatty acid methyl esters (FAMEs).
The classification of cave minerals and speleothems (1995)

The classification scheme of Hill and Forti, as used in the second edition of Cave Minerals of the World, is presented as a "practical" solution to the classification of cave minerals and speleothems. Classification and naming of cave minerals is by crystal class and follows nomenclature approved by the International Mineralogical Association. Classification of speleothems is based on morphology and whatever is known about origin, with division of speleothems into types, subtypes, and varieties. It is proposed that new speleothem types, subtypes, and names be approved by a UIS Commission of cave mineralogists.
The Reaction of Stock Market Returns to Unemployment (2017)

We empirically investigate the short-run impact of anticipated and unanticipated unemployment rates on stock prices. We particularly examine the nonlinearity in the stock market's reaction to the unemployment rate and study the effect at each individual point (quantile) of the stock return distribution. Using nonparametric Granger causality and quantile regression-based tests, we find that only anticipated unemployment rate has a strong impact on stock prices. Quantile regression analysis shows that the causal effects of anticipated unemployment rate on stock returns are usually heterogeneous across quantiles. For the quantile range (0.35, 0.80), an increase in the anticipated unemployment rate leads to an increase in stock market prices. For other quantiles, the impact is generally statistically insignificant. Thus, an increase in the anticipated unemployment rate is, in general, good news for stock prices. Finally, we offer a reasonable explanation for the reason, and manner in which, the unemployment rate affects stock market prices. Using the Fisher and Phillips curve equations, we show that a high unemployment rate is followed by monetary policy action of the Federal Reserve (Fed). When the unemployment rate is high, the Fed decreases the interest rate, which in turn increases the stock market prices.
Case History Review of the Application of Pressure Transient Testing and Production Logging in Monitoring the Performance of the Mars Deepwater Gulf of Mexico Field (2008)

Abstract Proper characterization of a producing well has significant impact on asset management. Production schedules from different wells and further infill drilling in a field depend on the effectiveness and the pattern of the production drive mechanism. Whether the reservoir displays partial aquifer influx or is waterflooded, the flow profile of produced/injected water as well as the reservoir pressure, permeability, fluid saturations, and formation compaction are useful to properly evaluate the overall sweep efficiency. This information is more crucial in a field with multi-layered reservoirs having different permeability, pressures, and structure. Data acquisition should be scheduled and compared to baseline data such as a baseline pulsed neutron capture, production, or compaction logs. There should also be plans to monitor specific wells over the life of the project to observe and optimize the reservoir performance, characterize the reservoir response, and in the case of waterflooded reservoirs monitor the performance of the waterflood. Multi-rate multi-zone production logging and pressure transient testing are two excellent methods to achieve these goals.
An electronically tunable lumped-element bandpass filter with continuous tuning of center frequency and bandwidth (2016)

Tunability of lumped-element bandpass filters on series LC-tanks with variable capacitors is analyzed. The filters are shown capable to tune continuously the center frequency and bandwidth. Conditions and constraints for keeping absolute bandwidth (ABW) and fractional bandwidth (FBW) constant are presented. Design of a three-pole varactor-tuned bandpass filter that allows a continuous tuning of the center frequency and bandwidth as well as keeping either ABW or FWB constant is demonstrated.
ChemInform Abstract: Photochemistry and Photophysics of Carbocations (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The Role of Basal Cells in Adhesion of Columnar Epithelium to Airway Basement Membrane (1988)

In this report, we present a new concept of the role of the basal cell in airway epithelium. Previously, the basal cell was thought to be the progenitor cell for the columnar epithelium. However, several studies have shown that this concept may not be correct. The morphologic aspects of the basal cell suggest that it could play a role in adhesion of the columnar epithelium to the basement membrane. Basal cells form attachments with columnar cells (desmosomes) and with the basement membrane (hemidesmosomes). Columnar cells do not form hemidesmosome attachments with the basement membrane. Basal cells could strengthen the adhesion of columnar cells to the basement membrane by forming hemidesmosome attachments to the basement membrane and desmosome attachments with adjacent columnar cells. Incidental evidence from 2 existing publications concerning airway microanatomy support this concept. As columnar cells grow taller, the proportion of the cell surface in contact with the basement membrane becomes progressively smaller, and thus the cell surface area related to adhesion also becomes smaller. It was found that the number of basal cells per millimeter of basement membrane was closely related to the height of the columnar cell epithelium (r = 0.98), but not to the number of columnar cells (r = 0.42). The consistency of the relationship between increased columnar cell height (and thus decreased surface area for adhesion) and the number of basal cells present (r = 0.98) supports the concept that the basal cell plays a role in adhesion of columnar cells to the basement membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
Growth and dissolution of potash alum crystals in the subsieve size range (1976)

Abstract Overall growth and dissolution rates of potash alum crystals have been measured over the size range 3 to 70 μm. The derived surface integration growth kinetics, which are found to be linear with respect to supersaturation and strongly size dependent, are evaluated in terms of the step model of crystal growth. The results emphasize the importance of independently measuring growth kinetics before secondary nucleation kinetics can be calculated from MSMPR experiments.
Structure of a paramagnetic supermicroporous silica‐based material via a multinuclear solid‐state NMR monitoring (2006)

The paramagnetic Si/Al material doped with 19.8% (weight) of Mn2+ has been characterized by the 1H, 13C, 27Al, 29Si MAS NMR spectra and the 1H, 29Si T1MAS relaxation measurements as a gel dried at 130 degrees C and as samples calcined at 504, 540 and 650 degrees C. It has been demonstrated that in spite of strong paramagnetic effects, NMR monitoring in combination with 29Si T1 relaxation experiments can be successful for structural descriptions of porous materials.
[Automated analysis of cardiotocographic signals]. (1992)

Cardiotocographic signals, used as a perinatal diagnostic tool, comprise fetal heart beat signals (FHR), uterine contractions (UC) and fetal movements (FM). Visual inspection of these three signals is limited, subjective, time consuming and with low reproducibility. The present paper illustrates an application of signal processing techniques to the automatic analysis of cardiotocographic signals, allowing to overcome visual inspection limitations. Porto system of cardiotocograms automatic analysis acquires the signals from a conventional cardiotocograph. The signals are stored and processed by a personal computer. System software allows the user to perform several operations such as signal acquisition, signal storage and retrieval from files, signal analysis and display. Signal analysis is preceded by various signal conditioning operations (filtering, spike removal, etc.) and consists in the estimation of several parameters with diagnostic value: FHR baseline; FHR accelerations and decelerations; uterine contractions; long and short term FHR variability. The system prototype is working on a routine basis at the Obstetrics Department of S. João Hospital (main Oporto Hospital) and is being evaluated using a large data set. A preliminary evaluation performed by 3 experts on a 50 cases set, yielded an agreement rate with computer measurements of 98% for the baseline, 72% to 82% for accelerations and decelerations and 76% for the uterine contractions.
Direct Injection of Ultrashort Electron Bunches Into a Solid Material Using Terahertz-Driven Electron Field Emission (2018)

We present here how terahertz (THz)-driven electron field emission from gold antennas can be directly injected into a solid material without any intermediate vacuum path. Simulations show that an ultrashort sub-ps driving THz laser pulse results in even shorter sub-ps electron bunches with up to 420 eV energies under vacuum conditions. An experiment shows that these electron bunches can be formed and accelerated directly inside the bulk of a solid under standard laboratory conditions (SLC). This is observed via the interaction of the electrons with scintillating molecules, which are placed inside the solid. Our result is a key step towards building a novel platform for femtochemistry driven by ultrashort electron bunches.
Real Life Radio-Location Examples For Enhanced Signal Processing Teaching (2018)

Publication in the conference proceedings of EUSIPCO, Kos island, Greece, 2017
Long-Term Planning (2005)

The objective of this chapter is to provide an overview of the transportation planning process and highlights the considerations inherent in effective long-term planning. Long-term transportation planning assesses existing conditions, identifies the future demand for travel, generates alternative strategies to support the projected demand, evaluates those alternatives and recommends to decision-makers a practical portfolio of policies, programs, and investments that would maintain and improve the mobility of people and the movement of goods, while supporting the area’s goals and objectives. In order to be effective, a long-term transportation plan must be grounded in the community’s vision, must reflect early and frequent public involvement, must have a comprehensive systems perspective, must be developed with strong technical methods and the cooperation of the host of transportation agencies, and must rest on realistic financial projections.
Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract (2018)

These results suggest that Pg-C-EE may have antimelanogenesis properties and skin-protective properties through regulation of activator protein 1 and cyclic adenosine monophosphate response element-binding protein signaling. Pg-C-EE may be used as a skin-improving agent, with moisture retention and whitening effects.
Leveraging mouse genetics to generate heavy-chain only antibodies for therapeutic application (2022)

Abstract Conventional antibodies (Abs) consist of two heavy and two light immunoglobulin chains, while heavy-chain only antibodies (HCAbs), like those found in camelid and shark species, are unique because they lack a light chain binding partner. The unique structure of these HCAbs impart advantageous properties to these smaller Abs, including greater accessibility to their target antigens, higher hydrophilicity, and greater conformational stability under environmental stresses. We have generated a mouse model, the IgG3dCH1 strain, that can produce HCAbs from the endogenous murine heavy chain locus. We have shown that these mice undergo efficient B cell development in the bone marrow, and generate a diverse repertoire of B cells that populate all peripheral immune compartments. While single-cell sequencing analysis of B cells revealed some biases in VH and JH usage, it also highlighted the tremendous diversity of rearrangements that give rise to B cells bearing these single chain antibodies. We’ve been able to show that upon immunization, IgG3dCH1 animals are protected from lethal bacterial challenge with a pathogenic strain of Enterobacter hormaechei and generate pathogen specific IgG. Further, immunization with SARS-CoV2 stabilized prefusion spike protein elicited HCAbs with a KD in the nM range, measured via bio-layer interferometry. These data support this mouse being utilized as a platform for the generation of pathogen-specific HCAbs that can be further engineered for testing and application for translational outcomes. Supported by grants from NIH (T32 AI007180, R43 AI136141).
Acute and subacute effects of the ultrasonic blade and electrosurgery on nerve physiology (2015)

Ultrasonic blades have been shown to cause less acute electrophysiological damage when applied near nerves than monopolar electrosurgery (ES). This study was performed to determine whether the acute nerve damage observed for ES, as well as the relative lack of damage observed for ultrasonic dissection, extends through a subacute timeframe. Muscle incisions were made in rat with the Harmonic(®) Blade (HB) and ES at a distance of 2 mm from the sciatic nerve. Sham surgery was also performed which consisted of similar exposure of the sciatic nerve without use of an energized device. Electrophysiological function was assessed acutely over a 3-h period, and subacutely after a 7-day survival, by monitoring the sciatic nerve compound action potential (CAP), conduction velocity (CV), von Frey hair (VFH) stimulation force, leukocyte infiltration, and impaired axonal transport via β-amyloid precursor protein (β-APP) immunocytochemistry. During the acute period, ES produced significantly lower CAP and CV, and higher levels of leukocytes and β-APP than sham, whereas the ultrasonic blade was not significantly different from sham, and had significantly lower VFH force than ES. After the subacute survival, ES continued to display significantly lower CAP and CV, and higher levels of leukocytes and β-APP than sham, whereas ultrasonic blade had higher CAP and CV than sham, and lower VFH than ES. This study confirms that incisions made with an ultrasonic blade cause less acute nerve damage than monopolar ES, and are comparable to sham surgery at a distance of 2 mm from the sciatic nerve. The negative effects of electrosurgery extend through at least a 7-day survival period, whereas subacute recovery after application of the ultrasonic blade was comparable to that of sham surgery. For surgical procedures in the vicinity of vital nerves, use of the ultrasonic blade represents a lower risk than ES for both acute and subacute neural trauma.
VALIDATION OF THE COUPLED PC-CRASH - MADYMO OCCUPANT SIMULATION MODEL. IN: OCCUPANT AND VEHICLE RESPONSES IN ROLLOVERS (2004)

A detailed study of vehicle and occupant movement during an accident is often of great interest. It allows investigators to validate injuries and determine their origin and offers detailed information regarding the influence of different restraint systems on the injury potential for occupants. This chapter, from a comprehensive text on vehicle and occupant responses in rollovers, discusses the validation of the coupled PC-CRASH and MDAYMO occupant simulation model. The authors report on a special series of sled tests that were performed to study the movement of the passenger during and after the collision; the tests were performed on an active sled at various test speeds. In addition, the authors report on a set of full scale tests that were performed with a volunteer stunt driver overturning the vehicles under various conditions. These test results were complemented by data from a standard Hybrid III test dummy sitting beside the stunt driver. Results from these tests demonstrated a large difference in the movement between human and the Hybrid III, primarily due to the active movement of the human driver and the long duration of the roll over. The authors conclude with a brief discussion of the use of this simulation method on multiple occupants and for a more flexible design of the interior.
G77-378 Odor Controls as Affected by Nuisance Laws (1977)

Confined feeding operations are subject to both public and private air pollution regulation. Public regulation is focused primarily on preventing measurable levels of emissions to the atmosphere. On the other hand, subjective human tolerance levels serve as the basis for private regulation of the environment. The impact of public and private regulatory schemes on feedlots will be reviewed in this NebGuide.
Liver Abscess Caused by Infection with Community-AcquiredKlebsiella quasipneumoniaesubsp.quasipneumoniae (2016)

We report a case of pyogenic liver abscess caused by community-acquired Klebsiella quasipneumoniae subsp. quasipneumoniae. The infecting isolate had 2 prominent features of hypervirulent K. pneumoniae strains: the capsular polysaccharide synthesis region for K1 serotype and the integrative and conjugative element ICEKp1, which encodes the virulence factors yersiniabactin, salmochelin, and RmpA.
Self-consistent estimation of inter-residue protein contact energies based on an equilibrium mixture approximation of residues (1999)

Pairwise contact energies for 20 types of residues are estimated self-consistently from the actual observed frequencies of contacts with regression coefficients that are obtained by comparing "input" and predicted values with the Bethe approximation for the equilibrium mixtures of residues interacting. This is premised on the fact that correlations between the "input" and the predicted values are sufficiently high although the regression coefficients themselves can depend to some extent on protein structures as well as interaction strengths. Residue coordination numbers are optimized to obtain the best correlation between "input" and predicted values for the partition energies. The contact energies self-consistently estimated this way indicate that the partition energies predicted with the Bethe approximation should be reduced by a factor of about 0.3 and the intrinsic pairwise energies by a factor of about 0.6. The observed distribution of contacts can be approximated with a small relative error of only about 0.08 as an equilibrium mixture of residues, if many proteins were employed to collect more than 20,000 contacts. Including repulsive packing interactions and secondary structure interactions further reduces the relative errors. These new contact energies are demonstrated by threading to have improved their ability to discriminate native structures from other non-native folds.
Vibrio vulnificus RtxA1 cytotoxin targets filamin A to regulate PAK1- and MAPK-dependent cytoskeleton reorganization and cell death (2019)

Cytoskeletal rearrangement and acute cytotoxicity occur in Vibrio vulnificus-infected host cells. RtxA1 toxin, a multifunctional autoprocessing repeats-in-toxin (MARTX), is essential for the pathogenesis of V. vulnificus and the programmed necrotic cell death. In this study, HeLa cells expressing RtxA1 amino acids 1491–1971 fused to GFP were observed to be rounded. Through yeast two-hybrid screening and subsequent immunoprecipitation validation assays, we confirmed the specific binding of a RtxA11491–1971 fragment with host-cell filamin A, an actin cross-linking scaffold protein. Downregulation of filamin A expression decreased the cytotoxicity of RtxA1 toward host cells. Furthermore, the phosphorylation of JNK and p38 MAPKs was induced by the RtxA1-filamin A interaction during the toxin-mediated cell death. However, the phosphorylation of these MAPKs was not observed during the RtxA1 intoxication of filamin A-deficient M2 cells. In addition, the depletion of pak1, which appeared to be activated by the RtxA1-filamin A interaction, inhibited RtxA1-induced phosphorylation of JNK and p38, and the cells treated with a pak1 inhibitor exhibited decreased RtxA1-mediated cytoskeletal rearrangement and cytotoxicity. Thus, the binding of filamin A by the RtxA11491–1971 domain appears to be a requisite to pak1-mediated MAPK activation, which contributes to the cytoskeletal reorganization and host cell death.