gebhart/patrepeval huggingface dataset:
Proteomic Analysis of Proteins Associated with Lipid Droplets of Basal and Lipolytically Stimulated 3T3-L1 Adipocytes (2004)

Adipocytes hold the body's major energy reserve as triacylglycerols packaged in large lipid droplets. Perilipins, the most abundant proteins on these lipid droplets, play a critical role in facilitating both triacylglycerol storage and hydrolysis. The stimulation of lipolysis by beta-adrenergic agonists triggers rapid phosphorylation of perilipin and translocation of hormone-sensitive lipase to the surfaces of lipid droplets and more gradual fragmentation and dispersion of micro-lipid droplets. Because few lipid droplet-associated proteins have been identified in adipocytes, we isolated lipid droplets from basal and lipolytically stimulated 3T3-L1 adipocytes and identified the component proteins by mass spectrometry. Structural proteins identified in both preparations include perilipin, S3-12, vimentin, and TIP47; in contrast, adipophilin, caveolin-1, and tubulin selectively localized to droplets in lipolytically stimulated cells. Lipid metabolic enzymes identified in both preparations include hormone-sensitive lipase, lanosterol synthase, NAD(P)-dependent steroid dehydrogenase-like protein, acyl-CoA synthetase, long chain family member (ACSL) 1, and CGI-58. 17-beta-Hydroxysteroid dehydrogenase, type 7, was identified only in basal preparations, whereas ACSL3 and 4 and two short-chain reductase/dehydrogenases were identified on droplets from lipolytically stimulated cells. Additionally, both preparations contained FSP27, ribophorin I, EHD2, diaphorase I, and ancient ubiquitous protein. Basal preparations contained CGI-49, whereas lipid droplets from lipolytically stimulated cells contained several Rab GTPases and tumor protein D54. A close association of mitochondria with lipid droplets was suggested by the identification of pyruvate carboxylase, prohibitin, and a subunit of ATP synthase in the preparations. Thus, adipocyte lipid droplets contain specific structural proteins as well as lipid metabolic enzymes; the structural reorganization of lipid droplets in response to the hormonal stimulation of lipolysis is accompanied by increases in the relative mass of several proteins and the recruitment of additional proteins.
Outcomes of Double Unit Umbilical Cord Blood Transplantation Using Fludarabine/Busulfan Based Reduced Intensity Conditioning Regimen (2014)

Umbilical cord blood (UCB) is increasingly used as a source of unrelated donor hematopoietic cells. Advantages of UCB include immediate graft availability, higher likelihood of acceptable match, and reduced likelihood and severity of graft vs. host disease (GVHD). Incorporation of Busulfan (Bu) with Fludarabine (Flu) in a reduced intensity conditioning (RIC) regimen has the potential advantages of improved disease control and acceptable toxicity. Eighteen patients (pts), median age 57 years (yrs) (range 27-70), median weight 83 kg (range 54-127), Karnofsky performance status 80-100%, underwent double unit UCB transplantation for hematologic malignancies (AML 44%; ALL 22%) between January 2012 and February 2013. Early disease was seen in 44%, intermediate 12%, and advanced 44%. Adverse cytogenetics were present in 39%. CMV seropositivity was seen in 16/18 (89%) pts. Hematopoietic cell transplantation comorbidity index (HCT-CI) scores were HCT-CI score 0, 44%; score 1-2, 44%; and score > 3, 12%. UCB units were 4-6/6 HLA-A, -B antigen, and DRB1 allele matched to the pt, with median 4.36 x 107 total nucleated cells(TNC)/kg (range 2.86–6.93) per transplant. RIC regimen included Bu 0.8 mg/kg IV q6h x 8 doses, Flu 35 mg/m2 IV/day x 5, TBI 300-450cGy total, and ATG 1.5 mg/kg/day x 3. Cyclosporine and mycophenolate mofetil were given for GVHD prophylaxis. Median follow up of surviving pts was 12 months. Day 180 overall survival (OS) was 72%. One yr OS and non-relapse mortality (NRM) were 45% and 49%, respectively. Relapse occurred in 1 pt. Cumulative incidence of myeloid and platelet engraftment was 83% and 72%, median 20 and 47 days (d), respectively. First chimerism evaluation occurred at median 21 d, all evaluable patients had ≥95% donor chimerism. At day 100, 79% of evaluable pts had single UCB unit dominance. Acute GVHD grades II-IV occurred in 39% and grades III-IV in 6% of patients. Chronic GVHD occurred in 17% during the follow up period. CMV reactivation occurred in 9/15 at risk pts, median 41 d post-transplant (range 18-54). A median of 3 CMV reactivations per pt was observed. Two died with CMV disease during first reactivation, while 2 others developed ganciclovir resistance. EBV reactivation occurred in 5/17 pts, 1 pt developed Post Transplant Lymphoproliferative Disorder (PTLD) unresponsive to therapy. One death was due to disseminated mucor. Flu/Bu based RIC regimen for double unit UCB transplantation is associated with rapid donor chimerism and possibly reduced disease relapse. Acute GVHD incidence was low. Failure to achieve neutrophil and platelet engraftment and high incidence of viral reactivation led to significant morbidity and high 1 yr NRM. Definitive conclusions based on the data are limited by small patient numbers and short follow up. Further strategies are needed to improve immune function after RIC double unit UCB transplant.
Scanning two-photon continuous flow lithography for synthesis of high-resolution 3D microparticles (2018)

An error in a reference is reported and corrected.
Evidence for the presence of chondrule‐ and CAI‐derived material in an isotopically anomalous Antarctic micrometeorite (2020)

Abstract We report the discovery of a unique, refractory phase‐bearing micrometeorite (WF1202A‐001) from the Sør Rondane Mountains, East Antarctica. A silicate‐rich cosmic spherule (~400 µm) displays a microporphyritic texture containing Ca‐Al‐rich inclusion (CAI)‐derived material (~5–10 area%), including high‐Mg forsterite (Fo 98‐99 ) and enstatite (En 98‐99 , Wo 0‐1 ). The micrometeorite also hosts a spherical inclusion (~209 µm), reminiscent of chondrules, displaying a barred olivine texture. Oxygen isotopic compositions of the micrometeorite groundmass (δ 17 O = –3.46‰, δ 18 O = 10.43‰, ∆ 17 O = –1.96‰) are consistent with a carbonaceous chondrite precursor body. Yet, a relict forsterite grain is characterized by δ 17 O = –45.8‰, δ 18 O = –43.7‰, ∆ 17 O = –23.1‰, compatible with CAIs. In contrast, a relict low‐Ca pyroxene grain (δ 17 O = –4.96‰, δ 18 O = –4.32‰, ∆ 17 O = –2.71‰) presumably represents a first‐generation silicate grain that accreted 18 O‐rich gas or dust in a transient melting scenario. The spherical inclusion displays anomalous oxygen isotope ratios (δ 17 O = –0.98‰, δ 18 O = –2.16‰, ∆ 17 O = 0.15‰), comparable to anhydrous interplanetary dust particles (IDPs) and fragments from Comet 81P/Wild2. Based on its major element geochemistry, the chondrule size, and oxygen isotope systematics, micrometeorite WF1202A‐001 likely sampled a carbonaceous chondrite parent body similar to, but distinct from CM, CO, or CV chondrites. This observation may suggest that some carbonaceous chondrite bodies can be linked to comets. The reconstructed atmospheric entry parameters of micrometeorite WF1202A‐001 suggest that the precursor particle originated from a low‐inclination, low‐eccentricity source region, most likely either the main belt asteroids or Jupiter family comets (JFCs).
Comparison of 2 endothelin-receptor antagonists on in vitro responses of equine palmar digital arterial and venous rings to endothelin-1. (2006)

The goals of this study were to determine the concentration-response (C-R) relationship of endothelin-1 (ET-1), compare 2 ET-receptor antagonists and determine the antagonist concentrations that block the vasomotor effects of ET-1, and compare the effectiveness of ET-1 and previously studied vasoconstrictors in equine palmar digital arterial and venous rings in vitro. Vessel rings from 8 nonlaminitic horses were placed in Tyrode's solution, 1 side fixed to the floor of an organ bath and the other side fixed to a force-displacement transducer. Two separate studies were conducted: (I) incubation with a single ET-receptor antagonist (PD142893 or PD145065 at a concentration of 10(-7), 10(-6), or 10(-5) M), followed by determination of an ET-1 C-R curve (using concentrations of 10(-10) to 10(-6) M) for medial vessel rings; and (II) comparison of ET-1 with norepinephrine and histamine (10(-10) to 10(-6) M) and comparison of contractile responses of medial and lateral vessel rings. In study I, ET-1 administration caused pronounced and sustained concentration-dependent contraction of vessel rings; these contractile responses were decreased by 10(-5) M PD142893 and were completely blocked by 10(-5) M PD145065. Venous rings had greater apparent maximum contraction in response to ET-1 than arterial rings. In study II, the relative sensitivity of norepinephrine was found to be equivalent to that of ET-1, whereas that of histamine was lower. No significant differences were observed between responses of medial versus lateral vessel rings. Thus, ET-1 is a potent vasoconstrictor of equine palmar digital arteries and veins, and the ET-receptor antagonist PD145065 is more effective than PD142893 in inhibiting these contractile effects in vitro.
Querying Evolving Graphs with Portal (2016)

Graphs are used to represent a plethora of phenomena, from the Web and social networks, to biological pathways, to semantic knowledge bases. Arguably the most interesting and important questions one can ask about graphs have to do with their evolution. Which Web pages are showing an increasing popularity trend? How does influence propagate in social networks? How does knowledge evolve? This paper proposes a logical model of an evolving graph called a TGraph, which captures evolution of graph topology and of its vertex and edge attributes. We present a compositional temporal graph algebra TGA, and show a reduction of TGA to temporal relational algebra with graph-specific primitives. We formally study the properties of TGA, and also show that it is sufficient to concisely express a wide range of common use cases. We describe an implementation of our model and algebra in Portal, built on top of Apache Spark / GraphX. We conduct extensive experiments on real datasets, and show that Portal scales.
Geographic distribution and evolution of yellow fever viruses based on direct sequencing of genomic cDNA fragments (1994)

International audience
Development and Validation of a Highly Sensitive and Specific Automated Algorithm to Evaluate the Abundance of Bubbles in Small Bowel Capsule Endoscopy (2017)

International audience
Pharmacologic P2X purinergic receptor antagonism in the treatment of collagen‐induced arthritis (2011)

Our findings indicate that P2X receptor antagonism has important therapeutic potential in chronic inflammatory rheumatic disorders. Taken together, our results underscore the value of the P2X receptor signaling pathway as a potential pharmacologic target for the modulation of adaptive immunity in CIA.
An architecture for automatic gesture analysis (2000)

The field of human-computer interaction has been widely investigated in the last years, resulting in a variety of systems used in different application fields like virtual reality simulation environments, software user interfaces, and digital library systems.A very crucial part of all these systems is the input module which is devoted to recognize the human operator in terms of tracking and/or recognition of human face, arms position, hand gestures, and so on.In this work a software architecture is presented, for the automatic recognition of human arms poses. Our research has been carried on in the robotics framework. A mobile robot that has to find its path to the goal in a partially structured environment can be trained by a human operator to follow particular routes in order to perform its task quickly. The system is able to recognize and classify some different poses of the operator's arms as direction commands like “turn-left”, “turn-right”, “go-straight”, and so on.A binary image of the operator silhouette is obtained from the gray-level input. Next, a slice centered on the silhouette itself is processed in order to compute the eigenvalues vector of the pixels co-variance matrix. This kind of information is strictly related to the shape of the contour of the operator figure, and can be usefully employed in order to assess the arms' position. Finally, a support vector machine (SVM) is trained in order to classify different poses, using the eigenvalues array.A detailed description of the system is presented along with some remarks on the statistical analysis we used, and on SVM. The experimental results, and an outline of the usability of the system as a generic shape classification tool are also reported.
Principles of 3′ splice site selection and alternative splicing for an unusual group II intron from Bacillus anthracis (2004)

We investigated the self-splicing properties of two introns from the bacterium Bacillus anthracis. One intron (B.a.I1) splices poorly in vitro despite having typical structural motifs, while the second (B.a.I2) splices well while having apparently degenerated features. The spliced exons of B.a.I2 were sequenced, and splicing was found to occur at a 3' site shifted one nucleotide from the expected position, thus restoring missing gamma-gamma' and IBS3-EBS3 pairings, but leaving the two conserved exonic ORFs out of frame. Because of the unexpected splice site, the principles for 3' intron definition were examined, which showed that the 3' splice site is flexible but contingent on gamma-gamma' and IBS3-EBS3 pairings, and can be as far away as four nucleotides from the wild-type site. Surprisingly, alternative splicing occurs at position +4 for wild-type B.a.I2 intron, both in vitro and in vivo, and the alternative event fuses the two conserved exon ORFs, presumably leading to translation of the downstream ORF. The finding suggests that the structural irregularities of B.a.I2 may be an adaptation to facilitate gene expression in vivo.
The OECD Program to Validate the Rat Hershberger Bioassay to Screen Compoundsfor in Vivo Androgen and Antiandrogen Responses. Phase 1: Use of a Potent Agonistand a Potent Antagonist to Test the Standardized Protocol (2006)

The Organisation for Economic Cooperation and Development (OECD) has completed phase 1 of the Hershberger validation intended to identify in vivo activity of suspected androgens and antiandrogens. Seventeen laboratories from 7 countries participated in phase 1, and results were collated and evaluated by the OECD with the support of an international committee of experts. Five androgen-responsive tissues (ventral prostate, paired seminal vesicles and coagulating glands, levator ani and bulbocavernosus muscles, glans penis, and paired Cowper's or bulbourethral glands) were evaluated. The standardized protocols used selected doses of a reference androgen, testosterone propionate (TP), and an antiandrogen, flutamide (FLU). All laboratories successfully detected TP-stimulated increases in androgen-responsive tissue weight and decreases in TP-stimulated tissue weights when FLU was co-administered. The standardized protocols performed well under a variety of conditions (e.g., strain, diet, housing protocol, bedding). There was good agreement among laboratories with regard to the TP doses inducing significant increases in tissue weights and the FLU doses decreasing TP-stimulated tissue weights. Several additional procedures (e.g., weighing of the dorsolateral prostate and fixation of tissues before weighing) and serum component measurements (e.g., luteinizing hormone) were also included by some laboratories to assess their potential utility. The results indicated that the OECD Hershberger protocol was robust, reproducible, and transferable across laboratories. Based on this phase 1 validation study, the protocols have been refined, and the next phase of the OECD validation program will test the protocol with selected doses of weak androgen agonists, androgen antagonists, a 5alpha-reductase inhibitor, and chemicals having no androgenic activity.
Increased nausea and dizziness when using tramadol for post-operative patient-controlled analgesia (PCA) compared with morphine after intraoperative loading with morphine (1998)

Thirty-eight ASA I-III patients undergoing lower abdominal operations were randomly allocated to receive either morphine (group M, patient-controlled analgesia bolus = 1 mg of morphine) or tramadol (group T, patient-controlled analgesia bolus = 10 mg of tramadol) for post-operative patient-controlled analgesia (PCA) after receiving morphine intraoperatively. There were no between-group differences in the pain, sedation or vomit scores. The nausea scores were significantly higher in group T in the initial 20 h and between 32 and 36 h (P < 0.01, 0-4 and 8-12 h; P < 0.05, 4-8, 12-16, 16-20 and 32-36 h). The incidence of dizziness was also significantly higher in group T (68.4% vs. 31.6%, group T vs. group M, P < 0.05). There was no difference in the overall satisfaction. We conclude that the use of tramadol, compared with morphine, for post-operative PCA after intraoperative loading with morphine is associated with more nausea and dizziness, but with similar sedation, quality of analgesia and patient satisfaction.
Efficient Complexation of N-Acetyl Amino Acid Carboxylates in Water by an Artificial Receptor:  Unexpected Cooperativity in the Binding of Glutamate but Not Aspartate (2005)

A new tris-cation 1 binds N-acetyl amino acid carboxylates in water even at millimolar salt concentrations with Kass approximately 103 M-1 due to a clustering of electrostatic interactions. Binding is efficient enough to allow a naked-eyed detection using an indicator displacement assay. Furthermore, receptor 1 shows an unexpected 2:1 complex formation with strong positive cooperativity with glutamate but not aspartate.
Polyphenols in mung bean (Vigna radiata (L.) Wilczek): determination and removal (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticlePolyphenols in mung bean (Vigna radiata (L.) Wilczek): determination and removalCharlene F. Barroga, Antonio C. Laurena, and Evelyn Mae T. MendozaCite this: J. Agric. Food Chem. 1985, 33, 5, 1006–1009Publication Date (Print):September 1, 1985Publication History Published online1 May 2002Published inissue 1 September 1985https://pubs.acs.org/doi/10.1021/jf00065a056https://doi.org/10.1021/jf00065a056research-articleACS PublicationsRequest reuse permissionsArticle Views820Altmetric-Citations80LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Rethinking The Data Collection Problem In Cyber Ranges . (2020)

Abstract not provided.
Anionic O → α- and β-Vinyl Carbamoyl Translocation of 2-(O-Carbamoyl) Stilbenes (2004)

[reaction: see text] New anionic oxygen to alpha- and beta-vinyl carbamoyl migration reactions, 17a and 26a-c --> 18 and 30a-c, proceed under LDA-mediated conditions leading stereoselectively to highly substituted stilbenes bearing electron-donating and -withdrawing substituents. Compounds 17a and 26a-c are prepared by combination of efficient, directed ortho metalation, Sonogashira, and Suzuki-Miyaura cross-coupling procedures.
Modelling local scour near structures with combined mesh movement and mesh optimisation (2018)

This paper develops a new implementation coupling optimisation-based anisotropic mesh adaptivity algorithms to a moving mesh numerical scour model, considering both turbulent suspended and bedload sediment transport. The significant flexibility over mesh structure and resolution, in space and time, that the coupling of these approaches provides makes this framework highly suitable for resolving individual marine structure scales with larger scale ocean dynamics. The use of mesh optimisation addresses the issue of poor mesh quality and/or inappropriate resolution that have compromised existing modelling approaches that apply mesh movement strategies alone, especially in the case of extreme scour. Discontinuous Galerkin finite element-based discretisation methods and a Reynolds Averaged Navier–Stokes-based turbulent modelling approach are used for the hydrodynamic fluid flow. In this work the model is verified in two dimensions for current-dominated scour near a horizontal pipeline. Combined adaptive mesh movement and anisotropic mesh optimisation is found to maintain both the quality and validity of the mesh in response to morphological bed evolution changes, even in the case where it is severely constrained by nearby structures.
The Palestinian Refugees’ “Right to Return” and the Peace Process (1997)

Within five years we will have six to seven million Arabs living on the West Bank and Jerusalem.All Palestinian Arabs will be welcomed by us.If the Jews can import all kinds of Ethiopians, Russians, Uzbekians, and Ukranians as Jews, we can import all kinds of Arabs.We plan to eliminate the State of Israel and establish a Palestinian state.We will make life unbearable for Jews by psychological warfare and population explosion.Jews will not want to live among Arabs.
ChemInform Abstract: Thermal Reactivity of Trimethylvinylsilane with Aryl Azides: First Examples of C‐Silylated 1,2,3‐Triazoline. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Frequency tuning of piezoelectric energy harvesters by magnetic force (2012)

A piezoelectric energy harvester is an electromechanical device that converts ambient mechanical vibration into electric power. Most existing vibration energy harvesting devices operate effectively at a single frequency only, dictated by the design of the device. This frequency must match the frequency of the host structure vibration. However, real world structural vibrations rarely have a specific constant frequency. Therefore, piezoelectric harvesters that generate usable power across a range of exciting frequencies are required to make this technology commercially viable. Currently known harvester tuning techniques have many limitations, in particular they miss the ability to work during harvester operation and most often cannot perform a precise tuning. This paper describes the design and testing of a vibration energy harvester with tunable resonance frequency, wherein the tuning is accomplished by changing the attraction force between two permanent magnets by adjusting the distance between the magnets. This tuning technique allows the natural frequency to be manipulated before and during operation of the harvester. Furthermore the paper presents a physical description of the frequency tuning effect. The experimental results achieved with a piezoelectric bimorph fit the calculated results very well. The calculation and experimental results show that using this tuning technique the natural frequency of the harvester can be varied efficiently within a wide range: in the test setup, the natural frequency of the piezoelectric bimorph could be increased by more than 70%.