gebhart/patrepeval huggingface dataset:
Overexpression of the Ubiquitin Specific Proteases USP43, USP41, USP27x and USP6 in Osteosarcoma Cell Lines: Inhibition of Osteosarcoma Tumor Growth and Lung Metastasis Development by the USP Antagonist PR619 (2021)

Osteosarcoma (OS) is the most common malignant bone tumor in children and teenagers. In many cases, such as poor response to treatment or the presence of metastases at diagnosis, the survival rate of patients remains very low. Although in the literature, more and more studies are emerging on the role of Ubiquitin-Specific Proteases (USPs) in the development of many cancers, few data exist regarding OS. In this context, RNA-sequencing analysis of OS cells and mesenchymal stem cells differentiated or not differentiated into osteoblasts reveals increased expression of four USPs in OS tumor cells: USP6, USP27x, USP41 and USP43. Tissue microarray analysis of patient biopsies demonstrates the nucleic and/or cytoplasmic expression of these four USPs at the protein level. Interestingly, Kaplan-Meyer analysis shows that the expression of two USPs, USP6 and USP41, is correlated with patient survival. In vivo experiments using a preclinical OS model, finally demonstrate that PR619, a USP inhibitor able to enhance protein ubiquitination in OS cell lines, reduces primary OS tumor growth and the development of lung metastases. In this context, in vitro experiments show that PR619 decreases the viability of OS cells, mainly by inducing a caspase3/7-dependent cell apoptosis. Overall, these results demonstrate the relevance of targeting USPs in OS.
Behaviour of flying oriental fruit moth males during approach to sex pheromone sources (1994)

Abstract. The pheromone‐modulated upwind flight tracks of Grapholita molesta (Busck) males were video recorded as they approached a point‐source of pheromone in a wind tunnel. The field of view of the video recording was divided longitudinally into 33 cm sections and the flight behaviour of the males in these sections was measured and compared as they approached from 233 cm to 50 cm downwind of the pheromone source. As the males approached the source, their mean ground speeds decreased. The mean values of their track angles increased with respect to due upwind (0d̀), indicating movement more across the wind. These changes resulted mainly from the males decreasing their air speeds as they progressed up the plume toward the source. They did not change the average direction of their steering (course angle). Thus, the increase in track angles resulted from the males allowing themselves to drift more in the wind as they approached the odour source. The males also increased their average rate of counterturning as they approached the source. The net result of all these behavioural changes was a track that slowed and grew narrower, giving the impression that the males were ‘homing‐in’ on the pheromone source as they approached. Causes of these systematic changes in behaviour are considered with respect to the known systematic changes in pheromone plume structure as the distance to the source decreases.
Printed S-Band 4 $\times$ 4 Nolen Matrix for Multiple Beam Antenna Applications (2009)

A Nolen matrix design method is proposed as an asymptotic singular case of a recent Blass matrix design algorithm. The procedure is illustrated by a 4 x 4 Nolen matrix, which design is centred at 2.2 GHz, with the amplitude and phase output excitation laws of a Butler matrix using 90deg hybrids. Simulation and measurement results are presented in this paper.
A Review of Risk Assessment Methods for Power System (2017)

In view of the current social background of power system becoming more and more complicated, and the current research situation of related fields, this paper discusses the common methods of power system risk assessment, including the method of hierarchy analysis, Markov chain model, Monte Carlo method, fuzzy comprehensive evaluation and related research progress. The advantages and disadvantages of the four methods are enumerated respectively. It is considered that the power system should be evaluated synthetically in the practical application and the effective risk assessment method should be adopted rationally.
2,4-D Amine Antagonism by Salts (1991)

Research was conducted to determine the influence of salts on 2,4-D toxicity to kochia. Calcium, magnesium, sodium, potassium, and iron salts except for sulfate and phosphate salts of calcium and sodium were antagonistic to 2,4-D diethanolamine. None of the ammonium salts antagonized 2,4-D control of kochia. Effects of individual ions generally antagonistic to 2,4-D were additive when in mixture. 2,4-D generally controlled kochia better when mixed with various acids than with their ammonium salts in distilled, sodium bicarbonate, or ferric sulfate water carriers, relating to the lower pH with the acids. However, low pH was not essential in overcoming salt antagonism of 2,4-D for kochia control, nor was 2,4-D always effective with low pH. Sulfate and monobasic phosphate anions were most effective in overcoming sodium bicarbonate and calcium chloride antagonism of 2,4-D. The concentration of diammonium sulfate needed to overcome sodium bicarbonate antagonism of 2,4-D increased with sodium bicarbonate concentration. Diammonium sulfate at 2% (w/v) overcame 1200 mg L –1 sodium as sodium bicarbonate. Nonionic surfactants and oil adjuvants also overcame antagonism of 2,4-D caused by water from several sources.
Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles (2003)

The binary Cre-lox conditional knockout system requires an essential part of the target gene to be flanked by loxP sites, enabling excision in vivo upon Cre expression. LoxP sites are introduced by homologous recombination, together with a selectable marker. However, this marker can disturb gene expression and should be removed. The marker is therefore often prepared with a third, flanking loxP site (tri-lox construct), facilitating its selective removal by partial Cre-lox recombination. We have shown that this excision can be achieved in vivo in the germline using EIIaCre transgenic mice, and have described the advantages of in vivo over in vitro removal. We show here that MeuCre40, a new transgenic mouse, more reliably and reproducibly generates an optimal partial mosaic Cre-lox recombination pattern in the early embryo. This mosaicism was transmitted to the germline and to many other tissues. Alleles with partial deletions, in particular floxed alleles from which the selectable marker was removed, were readily recovered in the next generation, after segregation from the transgene. Segregation via paternal or maternal transmission led to successful recovery of the alleles of interest. We also obtained total deletion of the floxed regions in the same experiment, making this transgene a polyvalent Cre-lox tool. We rigorously tested the ability of MeuCre40 to solve tri-lox problems, by using it for the in vivo removal of neo(R)- and hprt-expression cassettes from three different tri-lox mutants.
Experimental validation of a one-dimensional twin-entry radial turbine model under non-linear pulse conditions (2019)

This article presents the experimental validation of a complete integrated one-dimensional twin-scroll turbine model able to be used in reciprocating internal combustion engine unsteady simulations. A passenger car with a twin-entry-type turbine has been tested under engine-like pulse conditions by means of a specifically built gas stand. To obtain high-resolution quality data, the turbine and turbine line pipes have been instrumented with mean and instantaneous pressure sensors as well as temperature and mass flow sensors, employing a uniquely designed rotating valve for the pulse generation. This experimental configuration enables to obtain the pressure decomposition in both inlets and outlets of the turbine. Using the experimental data obtained, the model is fully validated, with special focus on the reflected and transmitted components for analysing the performance of the model and its non-linear acoustics prediction capabilities. The model presents a very high degree of correlation with the experimental results, providing a range of errors similar to the uncertainty of the measurements, even in the medium- and high-frequency spectra.
Long‐term clinical and radiographic evaluation after maxillary sinus floor augmentation with octacalcium phosphate–collagen composite: A retrospective case series study (2022)

The purpose of this study was to evaluate, using clinical and radiological assessments, the stability of dental implants 5 years after maxillary sinus floor augmentation with octacalcium phosphate-collagen composite (OCP/Col). Maxillary sinus floor augmentation was performed through a lateral window approach. Depending on the height of the host bone, a simultaneous approach (≥5 mm) or a staged approach (less than 5 mm) was employed. The primary outcome was the evaluation of clinical dental implant conditions such as infection, peri-implantitis, dental implant stability, pain, and paresthesia. Secondary outcomes were the evaluation of the augmented bone volume, change rate of augmented bone volume, vertical bone height, and marginal bone loss around dental implant fixture. The conditions of all dental implants were uneventful throughout the follow-up period. Augmented bone volume and changing rate of augmented bone volume were essentially unchanged following maturation of the OCP/Col-derived new bone. The change rate of new bone volume was 21.9% in the simulated approach and 16.8% in the staged approach at 1 year and 5 years postoperatively. The reduction rate in vertical bone height was 7.1% in the simultaneous approach and 7.5% in the staged approach between 1 year and 5 years postoperatively. Mean marginal bone loss was 1.76 mm with the simultaneous approach, and 0.50 mm with the staged approach at 5 years postoperatively. In conclusion, the success of dental implants 5 years after sinus floor augmentation by OCP/Col implantation was clarified by both clinical and radiological evaluations.
Data from NOTCH Is Part of the Transcriptional Network Regulating Cell Growth and Survival in Mouse Plasmacytomas (2023)

&lt;div&gt;Abstract&lt;p&gt;Aside from &lt;i&gt;Myc&lt;/i&gt;-activating translocations characteristic of plasmacytomas (PCT), little is known about genetic factors and signaling pathways responsible for the development of spontaneous B-cell lineage lymphomas of mice. Here, we characterized the transcriptional profiles of PCT, centroblastic diffuse large B-cell lymphomas (CBL), and high-grade splenic marginal zone B-cell lymphoma (MZL++) using high-throughput quantitative reverse transcription-PCR. Expression profiles of CBL and MZL++ were strikingly similar and quite unlike that of PCT. Among the genes expressed at significantly higher levels by PCT were a number involved in NOTCH signaling, a finding supported by gene set enrichment analyses of microarray data. To investigate the importance of this pathway, NOTCH signaling was blocked in PCT cell lines by treatment with a γ-secretase inhibitor (GSI) or transduction of a dominant-negative mutant of MAML1. These treatments resulted in reduced expression of NOTCH transcriptional targets in association with impaired proliferation and increased apoptosis. GSI treatment of transformed plasma cells in a primary PCT also induced apoptosis. These results integrate NOTCH activation with oncogenic signaling pathways downstream of translocated &lt;i&gt;Myc&lt;/i&gt; in the pathogenesis of mouse PCT, two signaling pathways also implicated in development of human multiple myeloma and T-cell lymphoblastic lymphoma. [Cancer Res 2008;68(22):9202–11]&lt;/p&gt;&lt;/div&gt;
Isotope Labeling-Assisted Evaluation of Hydrophilic and Hydrophobic Liquid Chromatograph–Mass Spectrometry for Metabolomics Profiling (2018)

High throughput untargeted metabolomics usually relies on complementary liquid chromatography-mass spectrometry (LC-MS) methods to expand the coverage of diverse metabolites, but the integration of those methods is not fully characterized. We systematically investigated the performance of hydrophilic interaction liquid chromatography (HILIC)-MS and nanoflow reverse-phase liquid chromatography (nRPLC)-MS under 8 LC-MS settings, varying stationary phases (HILIC and C18), mobile phases (acidic and basic pH), and MS ionization modes (positive and negative). Whereas nRPLC-MS optimization was previously reported, we found in HILIC-MS (2.1 mm × 150 mm) that the optimal performance was achieved in a 90 min gradient with 100 μL/min flow rate by loading metabolite extracts from 2 mg of cell/tissue samples. Since peak features were highly compromised by contaminants, we used stable isotope labeled yeast to enhance formula identification for comparing different LC-MS conditions. The 8 LC-MS settings enabled the detection of a total of 1050 formulas, among which 78%, 73%, and 62% formulas were recovered by the best combination of 4, 3, and 2 LC-MS settings, respectively. Moreover, these yeast samples were harvested in the presence or absence of nitrogen starvation, enabling quantitative comparisons of altered formulas and metabolite structures, followed by validation with selected synthetic metabolites. The results revealed that nitrogen starvation downregulated amino acid components but upregulated uridine-related metabolism. In summary, this study introduces a thorough evaluation of hydrophilicity and hydrophobicity based LC-MS and provides information for selecting complementary settings to balance throughput and efficiency during metabolomics experiments.
Proteomics in Nephrology (2004)

The novel discipline of proteomics has experienced a rapid growth in the recent past and has a great potential for the future. Involving several fields, it offers various advantages: The study of proteins on a genomic scale allows to examine a large number of them simultaneously. Moreover, proteomic analysis provides information about post-translational modifications and functions of gene products or proteins that cannot be obtained by genomic analysis. The knowledge thus gained is also important for a better understanding of renal physiology and pathophysiology, to identify disease biomarkers, and to develop new target therapies.This book is the first one that applies the science of proteomics to nephrology: It sets out to give an overview of the discipline, providing detailed background information and outlining scope, advantages, and limitations of proteomics.The second section reviews principles of commonly used proteomic techniques, including sample preparation, two-dimensional gel electrophoresis, various types of mass spectrometry, expression proteomics, quantitative proteomics, practical bioinformatics, and clinical proteomics. In the last section, examples and perspectives are provided of how proteomics can be applied to various fields in nephrology, including nephrotoxicity, sodium transport, diabetic nephropathy, renal Fanconi syndrome, IgA nephropathy, physiology of podocytes and mesangial cells, hypertension, urological malignancies, and dialysis. The publication ends with a discussion of biomarker discovery in glomerular diseases and drug discovery.Written by acclaimed experts in both proteomics and nephrology, this truly remarkable book is essential reading for students, residents, fellows, scientists, and nephrologists who are interested in or devoted to research, as well as for general health professionals and clinicians
Broadband spectroscopy of Bi(Mn0.33Nb0.67)O3.1ceramics (2019)

Results of broadband dielectric/electric investigations of Bi(Mn0.33Nb0.67)O3.1 ceramics are presented in wide frequency range 20 Hz–3 GHz. The electric properties of Bi(Mn0.33Nb0.67)O3.1 ceramics are mainly governed by high conductivity. The relaxation process, related to electrical conductivity, was detected. No polar phase transition was detected in Bi(Mn0.33Nb0.67)O3.1 ceramics by broadband spectroscopy. Bi(Mn0.33Nb0.67)O3.1 ceramics also not exhibit ferroelectric relaxor behavior.
Developmental Effect of Egg Pod Foam in the Desert Locust Schistocerca gregaria (Caelifera: Acrididae) (2002)

During the act of egg laying, in the desert locust Schistocerca gregaria (Forskal 1775), ♀♀ cover their eggs with a substance originating from the accessory glands. It hardens into a foamy material used as a chimney through which the delicate eclosed larvae can climb to the surface. The foam contains an as yet unidentified factor that promotes gregarisation. To distinguish the effect of this factor from that caused by another gregarisation promotory agent, namely the neuropeptide [His 7 ]-corazonin, a comparative developmental study was undertaken. Egg foam significantly influenced the development of both crowd- and isolated-reared hoppers. The development was longer if the eggs were washed and the factor was absent. The morphometrics were only slightly affected, and there was no effect on the coloration of the locusts.
Oceanibaculum nanhaiense sp. nov., isolated from surface seawater (2017)

A taxonomic study was carried out on strain L54-1-50<sup>T</sup>, which was isolated from surface seawater of the South China Sea. Cells of strain L54-1-50<sup>T</sup> were Gram-stain-negative, rod-shaped, oxidase-positive and catalase-positive. Growth was observed at salinities from 0 to 9 % (optimum 2 %, w/v), at pH 6.0-10.0 (optimum 8.0-9.0) and at temperatures from 10 to 45 °C (optimum 25-37 °C), but not at 4 or 50 °C. The 16S rRNA gene sequence analysis indicated that strain L54-1-50<sup>T</sup> was a member of the genus Oceanibaculum, related to Oceanibaculum indicum P24<sup>T</sup> (98.8 %) and Oceanibaculum pacificum MC2UP-L3<sup>T</sup> (97.7 %). The digital DNA-DNA hybridization values between strain L54-1-50<sup>T</sup> and the two type strains O. indicum P24<sup>T</sup> and O. pacificum MC2UP-L3<sup>T</sup> were 35.4±2.5 and 23.7±2.5 %, respectively. The average nucleotide identity values between strain L54-1-50<sup>T</sup> and two type strains were 79.7 and 88.3 %, respectively. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C18 : 1 2-OH. The respiratory quinone was Q-10. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, two unidentified phospholipids and three unidentified lipids. The G+C content of the chromosomal DNA was 65.1 mol%. The combined genotypic and phenotypic data showed that strain L54-1-50<sup>T</sup> represents a novel species of the genus Oceanibaculum, for which the name Oceanibaculumnanhaiense sp. nov. is proposed, with the type strain L54-1-50<sup>T</sup> (=KCTC 52312<sup>T</sup>=MCCC 1A05150<sup>T</sup>).
Electron spin resonance observation of a P-associated defect in HfO2 films on (100)Si (2007)

We report on the observation by electron spin resonance of P-related point defects in nm-thick HfO 2 films on (100)Si -an oxide prominent in current high-K insulator research. Based on the principal g matrices and hyperfine tensors inferred from consistent K and Q-band spectra simulations and comparison with established P-associated defects in silica, the center is assigned to a P 2 -type defect -a P substituting a Hf atom. The center is observed in the monoclinic phase of the HfO 2 oxide with the unpaired electron strongly localized on the P atom. Within the concern about dopant penetration out of Si into the high-K layers on top, identification of the dopant-associated defects in the latter appears crucial to which the present basic results provide fundamental access. The centers may operate as detrimental charge trapping sites.
Supramolecular polymers of 4,5-bis(bromomethyl)-1,3-dithiole-2-thione-dihalogen adducts (2004)

The title compound has been reacted with I2, IBr and ICl to afford the charge-transfer adducts 4·2I2, 4·IBr and 4·ICl·½I2, respectively. The products have been characterised by Raman and IR spectroscopy and X-ray crystallography. The adducts contain linear S⋯I–X (X = I, Br or Cl) units (174–178°) in which the S⋯I contacts range from 2.534–2.597 Å. In 4·2I2 and 4·ICl·½I2, the materials contain additional ‘free’ diiodine units which are held within a matrix of intermolecular contacts, predominantly through non-covalent halogen–halogen and sulfur–halogen interactions. Although the functional groups in the parent molecule (4) are inert at the supramolecular level, the bromomethyl groups in the adducts contribute strong interactions through Br⋯Br, Br⋯I, Br⋯S and Br⋯H close contacts. The structures of the complexes are polymeric in nature, providing interactions in two and three dimensions.
MATHEMATICAL SIMULATION OF HALOCARBON DISTRIBUTIONS IN BOSTON HARBOR. (1987)

In an effort to better understand the sources and transport of effluents, a combined modeling and field measurement program has been initiated. The modeling uses a pair of 2-D (depth-averaged) finite element models (one to compute circulation and one to compute transport) plus a number of supporting programs to facilitate grid generation, model interfacing, and plotting. Field measurements are made of a suite of volatile halocarbons discharged with the sewage effluent from the Deer and Nut Is. treatments plants and are used for model calibration. This paper reports on early results in a continuing study.
Anomalous Paramagnetism in Doped Carbon Nanostructures (2006)

The magnetic current flux induced in various types of carbon nanotori is studied and the paramagnetic and diamagnetic properties of carbon systems are explained as a function of electronic-band filling. By adding electrons or holes into the carbon structure it is possible to alter the band-filling configuration, thus modifying the magnetic response. When the electronic gap of these systems approaches zero, the local ring currents (either diamagnetic or paramagnetic, see image) are enhanced.
Joint Network/Channel Coding for Multi-User Hybrid-ARQ (2008)

We present a novel multi-user hybrid-ARQ scheme for a wireless multiple-unicast scenario where a base station supplies a group of users with individual messages. Considering that all users have acc ...
Back Cover: Highly Selective Condensation of Biomass‐Derived Methyl Ketones as a Source of Aviation Fuel (ChemSusChem 10/2015) (2015)

The Back Cover shows a depiction of the carbon cycle for a tunable set of chemistry that can be used to synthesize exceptional jet fuel via n-alkyl methyl ketones. In this work, methyl ketones, which can be derived from biomass in either chemical or hybrid biological–chemical pathways, are selectively transformed to cyclic trimers by aldol condensation and Michael addition. By altering the ratio of starting methyl ketones, an alkane blend can be generated after hydrodeoxygenation that not only satisfies the low freezing point necessary for jet fuel, but also the broad volatility distribution typical of petroleum-derived fuels. The carbon generated by combustion of these fuels then produces further biomass, completing the carbon cycle. More details can be found in the Full Paper by Sacia et al. (DOI: 10.1002/cssc.201500002).