gebhart/patrepeval huggingface dataset:
Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane (2021)

Genome editing with sequence-specific nucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is revolutionizing crop improvement. Developing efficient genome-editing protocols for highly polyploid crops, including sugarcane (<i>x</i> = 10-13), remains challenging due to the high level of genetic redundancy in these plants. Here, we report the efficient multiallelic editing of magnesium chelatase subunit I (<i>MgCh</i>) in sugarcane. Magnesium chelatase is a key enzyme for chlorophyll biosynthesis. CRISPR/Cas9-mediated targeted co-mutagenesis of 49 copies/alleles of magnesium chelatase was confirmed via Sanger sequencing of cloned PCR amplicons. This resulted in severely reduced chlorophyll contents, which was scorable at the time of plant regeneration in the tissue culture. Heat treatment following the delivery of genome editing reagents elevated the editing frequency 2-fold and drastically promoted co-editing of multiple alleles, which proved necessary to create a phenotype that was visibly distinguishable from the wild type. Despite their yellow leaf color, the edited plants were established well in the soil and did not show noticeable growth retardation. This approach will facilitate the establishment of genome editing protocols for recalcitrant crops and support further optimization, including the evaluation of alternative RNA-guided nucleases to overcome the limitations of the protospacer adjacent motif (PAM) site or to develop novel delivery strategies for genome editing reagents.
Effectiveness of early switching from intravenous to oral antibiotic therapy in Staphylococcus aureus prosthetic bone and joint or orthopedic metalware-associated infections (2021)

There was a low treatment failure rate in patients with S. aureus prosthetic bone and joint or orthopedic metalware-associated infection with early oral switch from intravenous to oral antibiotic therapy.
Artificial intelligence-assisted chiral nanophotonic designs (2023)

Chiral nanostructures can enhance the weak inherent chiral effects of biomolecules and highlight the important roles in chiral detection. However, the design of the chiral nanostructures is challenged by extensive theoretical simulations and explorative experiments. Recently, Zheyu Fang’s group proposed a chiral nanostructure design method based on reinforcement learning, which can find out metallic chiral nanostructures with a sharp peak in circular dichroism spectra and enhance the chiral detection signals. This work envisions the powerful roles of artificial intelligence in nanophotonic designs.
340 Convection-Enhanced Delivery of Macromolecules to the Brain Using Electrokinetic Transport (2018)

Faraji, Amir H MD, PhD; Jaquins-Gerstl, Andrea PhD; Valenta, Alec C; Ou, Yangguang PhD; Richardson, R. Mark MD; Weber, Stephen G PhD Author Information
A high-precision millimeter-wave navigation system for indoor and urban environment autonomous vehicles (2013)

This paper presents a mm-wave based navigation system capable of measuring and tracking a vehicle's position with centimeter scale accuracy. The system uses two 144 GHz transmitters modulated with 13 and 14 MHz tones combined with quasi-optical lenses to establish a mm-wave beam that defines navigational pathway for a vehicle to follow. The system is suitable for applications where precision guiding of small autonomous vehicles along a precise path is required such as navigating indoors or in cluttered urban environments.
Sorority blessed with the gifts of Mother Brown (2005)

Sigma Kappa housemother Norma Brown ’37 guided her “girls” through the tumultuous 1970's.
Sulphur isotope fractionation during the reduction of elemental sulphur and thiosulphate byDethiosulfovibriospp. (2012)

Stable sulphur isotope fractionation was investigated during reduction of thiosulphate and elemental sulphur at 28°C by growing batch cultures of the sulphur- and thiosulphate-reducing bacteria Dethiosulfovibrio marinus (type strain DSM 12537) and Dethiosulfovibrio russensis (type strain DSM 12538), using citrate as carbon and energy source. The cell-specific thiosulphate reduction rate in the growth phase was 7.4±3.9 fmol cell(-1) d(-1). The hydrogen sulphide produced was enriched in (32)S by 10.3±1 ‰ compared with total thiosulphate sulphur, close to previous experimental results observed for other sulphate- and non-sulphate-reducing bacteria. Elemental sulphur reduction yields sulphur isotope enrichment factors between-1.3 and-5.2 ‰ for D. russensis and-1.7 and-5.1 ‰ for D. marinus. The smaller fractionation effects are observed in the exponential growth phase (cellular rates between 5 and 70 fmol S° cell(-1) d(-1)) and enhanced discrimination under conditions of citrate depletion and cell lysis (cellular rates between 0.3 and 3 fmol S° cell(-1) d(-1)).
Conversational programming in action (2011)

Accelerated by the Do-It-Yourself mindset of the Web 2.0 culture, end-user programming, which is programming by end users with limited, if any, formal programming background, is growing rapidly. Especially in educational settings, children are exposed to computational thinking by making games, building scientific simulations and creating stories. Early educational programming languages such as Logo have made programming substantially more accessible to end users. More recent approaches include visual programming with drag-and-drop style of programming making it nearly impossible to compose syntactically incorrect programs. However, as the syntactic challenges of end-user programming are gradually fading into the past, the new frontier of semantic programming support emerges. This demonstration introduces Future Trace, a system to make programming more conversational. A conversational programming agent runs programs one step into the future in order to visualize discrepancies between the programs users intended to write and the actual programs.
Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge (2016)

In recent years, elk (Cervus canadensis) have been implicated as the source of Brucella abortus infection for numerous cattle herds in the Greater Yellowstone Area. In the face of environmental and ecological changes on the landscape, the range of infected elk is expanding. Consequently, the development of effective disease management strategies for wild elk herds is of utmost importance, not only for the prevention of reintroduction of brucellosis to cattle, but also for the overall health of the Greater Yellowstone Area elk populations. In two studies, we evaluated the efficacy of B. abortus strain RB51 over-expressing superoxide dismutase and glycosyltransferase for protecting elk from infection and disease caused by B. abortus after experimental infection with a virulent B. abortus strain. Our data indicate that the recombinant vaccine does not protect elk against brucellosis. Further, work is needed for development of an effective brucellosis vaccine for use in elk.
Nuclear And Electronic Spin Magnetic Resonance (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear And Electronic Spin Magnetic ResonanceJohn E. WertzCite this: Chem. Rev. 1955, 55, 5, 829–955Publication Date (Print):October 1, 1955Publication History Published online1 May 2002Published inissue 1 October 1955https://pubs.acs.org/doi/10.1021/cr50005a002https://doi.org/10.1021/cr50005a002research-articleACS PublicationsRequest reuse permissionsArticle Views613Altmetric-Citations104LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Part I: Physical Insights Into Dynamic R ON Behavior and a Unique Time-Dependent Critical Stress Voltage in AlGaN/GaN HEMTs (2021)

We report a unique OFF-state drain-to-source critical stress voltage above which the dynamic performance of AlGaN/GaN HEMTs is significantly deteriorated. Physical insights are developed through detailed experiments conducted for different design variants, increased substrate bias, varying temperature, varying stress time, and experimenting buffers with lower C-doping. The surface leakage measurements on dedicated test structures and experimentation of devices realized over GaN buffer with different carbon doping reveal that the observed phenomenon is solely caused by traps present in carbon-doped GaN buffer. Surface traps have no role to play as far as they do not modulate the electric field in the GaN buffer (discussed in Part-II). Stress time, substrate temperature, substrate bias, electric-field shape, and depletion regions formed under the gate as well as field plate region were found to play a key role in governing trap-assisted transport and carrier trapping in the GaN buffer. A deeper physical insight to explain the observed phenomena and its dependency on various conditions are developed through detailed measurements, TCAD simulations, electroluminescence, and photoluminescence spectroscopy. With the help of the physical insight gained into the underlying mechanism, a novel surface passivation scheme using a p-type oxide is reported in Part-II, which helps relaxing the field distribution across the access region and buffer, and in turn improves the dynamic performance of the HEMTs. Besides, the Part-II also unifies the mechanism across different gate-stack designs and reveals the interplay between the surface and buffer, which governs the dynamic performance of these devices.
Production of Potato Cyst Nematode Hatching Stimulus by Hairy Root Cultures of Tomato (1991)

Hairy root clones of tomato (Lycopevsicon esculentum) were obtained by inoculation of the stems of sterile plants with Agrobacterium rhizogenes. The transformed root cultures produced a potato cyst nematode hatching stimulus, which was detected both in the extract of the cultured roots and in th culture medium. The hatching stimulus was also produced by non-transformed tomato root cultures. Large portions of the hatching stimuli synthesized in the hairy and non-transformed roots were found to be released into the medium. Five independent clones of the tomato hairy root cultures displayed considerable variation in the production of stimulus.
ChemInform Abstract: Copper Chloride‐Cyanopyridine Complexes as Catalysts for the Decomposition of Fluorophosphonate Esters. (1989)

Abstract Several copper(II) pyridine complexes such as (IV) are prepared.
NFC Enabled Smart Postal System (2010)

This paper presents a smart postal application, where the Near Field Communication (NFC) technology is applied into the traditional postal system in order to create a new mailing experience. The newly invented smart postal system is capable of facilitating the mail procedure, thus improving the efficiency of the traditional postal system. Moreover by attaching a Radio Frequency Identification (RFID) tag into an envelop, a stamp or a parcel package, it permits users to combine digital media with a physical object, which creates a cross-media tangible mailing experience. The smart postal system consists of a local mobile postal application running on an NFC enabled mobile handheld and a network subsystem. The network subsystem comprises a smart postal agent service, a user profile service, a RFID correlation service and a payment service, where the smart postal agent service is the key invented network component. This is a loosely coupled network architecture that allows the functions of each network component to be split and re-arranged to fit into any specific existing system.
In vivo myocardial kinetics of air-filled albumin microbubbles during myocardial contrast echocardiography. Comparison with radiolabeled red blood cells. (1994)

Myocardial contrast echocardiography (MCE) is a new technique for assessing myocardial perfusion that uses intracoronary injections of microbubbles of air. Because these microbubbles have a mean diameter of 4.3 +/- 0.3 microns and an intravascular rheology similar to that of red blood cells (RBCs), we hypothesized that their mean myocardial transit rates recorded on echocardiography would provide an estimation of regional myocardial blood flow in the in vivo beating heart. Accordingly, blood flow to the left anterior descending coronary artery (LAD) of 12 open-chest anesthetized dogs (group I) was adjusted to 4 to 6 flows (total of 60 flows), and microbubbles and radiolabeled RBCs were injected into the LAD in a random order at each stage. The mean myocardial RBC transit rates were measured by fitting a gamma-variate function to time-activity plots generated by placing a miniature CsI2 probe over the anterior surface of the heart, and the mean myocardial microbubble transit rates were measured from time-intensity plots derived from off-line analysis of MCE images obtained during the injection of microbubbles. An excellent correlation was noted between flow (measured with an extracorporeal electromagnetic flow probe) and mean myocardial RBC transit rate (y = 2.83 x 10(-3)x + 0.01, r = .96, SEE = 0.02, P < .001). A close correlation was also noted between mean RBC and microbubble myocardial transit rates (y = 1.01x + 0.01, r = .89, SEE = 0.02, P < .001). Despite its theoretical advantages, a lagged normal density function did not provide a better fit to the MCE data than the gamma-variate function.(ABSTRACT TRUNCATED AT 250 WORDS)
Accurate and Robust Indoor Localization Systems using Ultra-wideband Signals (2013)

Abstract—Indoorlocalizationsystems that are accurate and robust with respect to propagation channel conditions are still a technical challenge today. In particular, for systemsbasedon rangemeasurementsfromradio signals, non-line-of-sight (NLOS) situations can result in large position errors. In this paper, we address these issues using measurements in a representative indoor environment. Results show that conventional tracking schemes using high- and a low-complexityranging algorithms are strongly impaired by NLOS conditions unless a very large signal bandwidth is used. Furthermore, we discuss and evaluate the performance of multipath-assisted indoor navigation and tracking (MINT), that can overcome these impairments by making use of multipath propagation. Across a wide range of bandwidths, MINT shows superior performance comparedto conventionalschemes,andvirtuallyno degradation in its robustness due to NLOS conditions.
Analysis of pectin mutants and natural accessions of Arabidopsis highlights the impact of de-methyl-esterified homogalacturonan on tissue saccharification (2013)

We propose to use the level of HGA domains as a biochemical marker of the cell wall recalcitrance to saccharification. This may be utilized for selecting, on a large scale, natural variants or mutants with improved bioconversion features.
Conflict of Interest Form from Inflammation-Related IL1β/IL1R Signaling Promotes the Development of Asbestos-Induced Malignant Mesothelioma (2023)

Conflict of Interest Form from Inflammation-Related IL1β/IL1R Signaling Promotes the Development of Asbestos-Induced Malignant Mesothelioma
Use of Very-Short-Lived Isotopes in Activation Analysis (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of Very-Short-Lived Isotopes in Activation AnalysisO. U. AndersCite this: Anal. Chem. 1961, 33, 12, 1706–1709Publication Date (Print):November 1, 1961Publication History Published online1 May 2002Published inissue 1 November 1961https://pubs.acs.org/doi/10.1021/ac60180a025https://doi.org/10.1021/ac60180a025research-articleACS PublicationsRequest reuse permissionsArticle Views32Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Enantioselective Intramolecular Copper‐Catalyzed Aziridination of Sulfamates. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Use of the bovine leukaemia virus LTR U3 promoter for expressing antisense antiviral RNAs and competitive inhibition of viral infection in cell culture. (1997)

Use of viral inducible promoters which can be activated by virus-specific transactivator proteins to drive expression of antisense (as)RNA genes appears to be an attractive approach to inhibit virus infections in vivo. To this end, we have constructed an asRNA gene expressed from the bovine leukaemia virus (BLV) U3 promoter that is complementary to the R-U5 region of the BLV genome. This is the region that is most susceptible to inhibition by asRNA. With plasmid pLU, which expresses the asRNA gene under the control of the BLV U3 promoter, 75% inhibition of virus replication was attained in CC81 cells (the molar ratio of pLU DNA over BLV proviral DNA in the transfection mixture was 5:1). Plasmid pLT, which contains only the BLV U3 promoter without any asRNA-coding region, also efficiently (up to 60%) inhibited virus replication when cotransfected with BLV proviral DNA at a ratio of 20:1. It was suggested that competition between functional and 'empty' viral promoters for the viral transactivator protein p38tax could account for this inhibition. An immunoblotting assay showed that in the presence of nuclear extracts from CC81 cells exogenous BLV p38tax specifically associates with its responsive sequence located in the BLV U3 promoter. Moreover, the additional expression of p38tax in CC81 cells abolishes the inhibitory effect of the empty viral promoter. These observations suggest a new mechanism of BLV inhibition caused, most probably, by sequestering of the viral transactivator protein.
Enhanced Electrocatalytic Activity of Pt Subnanoclusters on Graphene Nanosheet Surface (2009)

Graphene nanosheet (GNS) gives rise to an extraordinary modification to the properties of Pt cluster electrocatalysts supported on it. The Pt/GNS electrocatalyst revealed an unusually high activity for methanol oxidation reaction compared to Pt/carbon black catalyst. The Pt/GNS electrocatalyst also revealed quite a different characteristic for CO oxidation among the measured catalyst samples. It is found that Pt particles below 0.5 nm in size are formed on GNS, which would acquire the specific electronic structures of Pt, modifying its catalytic activities.
Role of 53BP1 oligomerization in regulating double-strand break repair (2013)

Tumor suppressor p53-binding protein 1 (53BP1) regulates the repair of dysfunctional telomeres lacking the shelterin protein TRF2 by promoting their mobility, their nonhomologous end-joining (NHEJ), and, as we show here, by blocking 5' resection by CtIP. We report that these functions of 53BP1 required its N-terminal ATM/ATR target sites and its association with H4K20diMe, but not the BRCT domain, the GAR domain, or the binding of 53BP1 to dynein. A mutant lacking the oligomerization domain (53BP1(oligo)) was only modestly impaired in promoting NHEJ of dysfunctional telomeres and showed no defect with regard to the repression of CtIP. This 53BP1(oligo) allele was previously found to be unable to support class switch recombination or to promote radial chromosome formation in PARP1 inhibitor-treated Brca1-deficient cells. The data therefore support two conclusions. First, the requirements for 53BP1 in mediating NHEJ at dysfunctional telomeres and in class switch recombination are not identical. Second, 53BP1-dependent repression of CtIP at double-strand breaks (DSBs) is unlikely to be sufficient for the generation of radial chromosomes in PARP1 inhibitor-treated Brca1-deficient cells.
Clinical Study Protocol for the ARCH Project Computational Modeling for Improvement of Outcome after Vascular Access Creation (2011)

Despite clinical guidelines and the possibility of diagnostic vascular imaging, creation and maintenance of a vascular access (VA) remains problematic: avoiding short- and long-term VA dysfunction is challenging. Although prognostic factors for VA dysfunction have been identified in previous studies, their potential interplay at a systemic level is disregarded. Consideration of multiple prognostic patient specific factors and their complex interaction using dedicated computational modeling tools might improve outcome after VA creation by enabling a better selection of VA configuration. These computational modeling tools are developed and validated in the ARCH project: a joint initiative of four medical centers and three industrial partners (FP7-ICT-224390). This paper reports the rationale behind computational modeling and presents the clinical study protocol designed for calibrating and validating these modeling tools. The clinical study is based on the pre-operative collection of structural and functional data at a vascular level, as well as a VA functional evaluation during the follow-up period. The strategy adopted to perform the study and for data collection is also described here.