gebhart/patrepeval huggingface dataset:
Characterization and function of catalytic subunit a of H/sup +/-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae (1988)

Subunit a (M/sub r/ 89,000) from vacuolar membrane H/sup +/-translocating adenosine triphosphatase of the yeast Saccharomyces cerevisiae was found to bind 8-azido(..cap alpha..-/sup 32/P)adenosine triphosphate. Labeling by this photosensitive ATP derivative was saturable with an apparent dissociation constant of 10/sup -6/ to 10/sup -5/ M and decreased in the presence of ATP and ADP. The enzyme was inactivated by 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), with about 1 ..mu..M causing half-maximal inactivation in the neutral pH range. This inactivation was prevented by the presence of ATP, ADP, or adenosyl-5'-yl imidodiphosphate (AMP-PNP). The original activity was restored by treating the inactivated enzyme with 2-mercaptoethanol. Kinetic and chemical studies of the inactivation showed that the activity was lost on chemical modification of a single tyrosine residue per molecule of the enzyme. When the enzyme was inactivated with (/sup 14/C)NBD-Cl, subunit a was specifically labeled, and this labeling was completely prevented by the presence of ATP, GTP, ADP, or AMP-PNP. From these results, it was concluded that subunit a of yeast vacuolar H/sup +/-ATPase has a catalytic site that contains a single, essential tyrosine residue. The kinetics of single site hydrolysis of (..gamma..-/sup 32/P)ATP indicated the formation of an enzyme-ATP complex and subsequent hydrolysis of bound ATPmore » to ADP and P/sub i/ at the NBD-Cl-sensitive catalytic site.« less
The Autoimmune Regulator (Aire) transactivates HLA‐G gene expression in thymic epithelial cells (2019)

The Autoimmune Regulator (Aire) protein coordinates the negative selection of developing thymocytes by inducing the expression of hundreds of tissue-specific antigens within the thymic medulla, which is also a primary site of the expression of the immune checkpoint HLA-G molecule. Considering the immunomodulatory properties of Aire and HLA-G, and considering that the role of the constitutive thymus expression of HLA-G has not been elucidated, we studied the effect of AIRE cDNA transfection on HLA-G expression in 4D6 thymic cells and in the HLA-G-positive JEG-3 choriocarcinoma cells. Aire promoted the transactivation of HLA-G gene by increasing the overall transcription, inducing the transcription of at least G1 and G2/G4 isoforms, and incrementing the occurrence and distribution of intracellular HLA-G protein solely in 4D6 thymic cells. Luciferase-based assays and chromatin immunoprecipitation experiments performed in 4D6 cells revealed that Aire targeted at least two regions within the 5'-untranslated regulatory region (5'-URR) extending 1·4 kb from the first ATG initiation codon. The interaction occurs independently of three putative Aire-binding sites. These results indicate that the Aire-induced upregulation of HLA-G in thymic cells is likely to act through the interaction of Aire with specific HLA-G 5'-URR DNA-binding factors. Such a multimeric transcriptional complex might operate in the thymus during the process of promiscuous gene expression.
Boosting the performance of energy harvesters subject to white Gaussian vibrations through impedance matching solutions (2022)

We discuss models of piezoelectric energy harvesters for scavenging energy from random mechanical vibrations, represented by a white Gaussian noise source. We show that circuit theory can be applied to design a matching network, to be interposed between the piezoelectric transducer and the load, able to increase the power transferred to the latter. The second order matching network is compared with a previously proposed solution, based on power factor correction, implemented through a single reactive element. We show that the new architecture boosts the harvested power with respect to the simple, unmatched resistive load, by a factor larger than 9, and with respect to the power factor correction solution by more than 17%.
Abstract WP64: Wrist Proprioception In The Subacute Period After Stroke (2023)

Background: Proprioception is critical to motor control. The prevalence, severity, and clinical correlates of deficits in wrist proprioception early after stroke have received limited attention and were examined in patients admitted to an inpatient rehabilitation facility (IRF). Methods: Patients admitted for stroke to our IRF (n=18) and older healthy controls (n=12) completed the Wrist Position Sense Test (WPST), a validated, quantitative proprioception measure, as well as motor and cognitive testing. Patients were serially tested when available (n=12). Results: In controls, mean (± SD) WPST error was 9.1 ± 3.6 deg in the dominant wrist and 9.2 ± 4.5 deg in the nondominant wrist. In patients with stroke, WPST error was 18.6 ± 9 deg in the more affected wrist, with abnormal values present in 88.2%; and 11.5 ± 5.6 deg in the less affected wrist, which was abnormal in 55.6% of patients. Error in the more affected wrist was higher than in the less affected wrist (p=0.003), dominant wrist of controls (p=0.001), and nondominant wrist of controls (p=0.003). Retesting in patients a mean of 11 days later found error was not significantly changed (17 ± 7.7 deg in more affected wrist and 15.6 ± 16.3 deg in less affected). Among controls, higher dominant hand WPST error correlated with poorer 9-hole peg (rho=0.75, FDR-corrected p=0.048) and Box &amp; Blocks (r=-0.77, FDR-corrected p=0.048) scores; these relationships were not seen in patients with stroke. WPST error in either hand of patients with stroke was not related to MoCA, NIHSS, or Fugl-Meyer scores. ConclusionS: Deficits in wrist proprioception are common early after stroke, with average error twice that of healthy controls. These differences are not explained by cognitive or motor deficits. Relationships that proprioception has with motor function in healthy subjects are disrupted early after stroke.
Starting a regional collaborative research group for COVID-19 in pregnancy: the Southern Michigan experience (2020)

The outbreak of the SARS-CoV-2 elicited a surge in publications. Obstetric reports were with few exceptions characterized by small sample sizes with potentially limited generalizability. In this review, evidence suggests increased susceptibility to COVID-19 in pregnancy; common pregnancy comorbidities may help explain worse outcomes. While the risk of death is low, pregnancy may be associated with increased need for ventilation. Prematurity rates seem to be increased but may be accounted for in part by higher cesarean rates, to a large degree accounted for by elective decision to shorten the course of the labor. Though fetal/neonatal complication rates may be higher in the presence of COVID-19 infection, survival rates seem unaffected and vertical transmission is rare. As the outbreak continues in the USA with resurgence in many other western countries that achieved initial success in suppressing the virus, much remains to be learned. For example, the question related to the degree to pregnancy modifying symptomatology remains open. Currently, routine polymerase chain reaction testing remains limited by supply shortages possibly delaying diagnosis until later in the course of the disorder and thus altering the symptom complex at presentation. To add to the knowledge base, we initiated a regional COVID-19 in pregnancy collaborative observational study with a coordinating center, standardized data collection and a shared database. This was facilitated by a longstanding tradition of collaboration among regional obstetric services. Over an anticipated two-year study duration, we expect to study 400 documented and suspected COVID-19 pregnancies with time and site of services controls for cohort effect and high power to detect several adverse maternal/infant outcomes. We include a complete listing of variables in our database, which, along with our experience in setting up our regional collaborative, we hope and believe will be of use in other settings.
Body dysmorphic disorder: the distress of imagined ugliness (1991)

More research on the nosology, clinical features, and treatment response of body dysmorphic disorder is important, given the distress and impairment this often secret disorder can cause.
A unique starting scheme of linear-engine tubular PM linear generator system using position feedback controlled PWM inverter (2006)

The paper provides a novel method to start the linear engine coupled linear generator from dead stop to its final steady state operation. This method depends mainly to use the linear generator mounted on the shaft of the linear engine to provide the required thrust force to move and oscillate the linear engine from bottom to top dead centers. It is a cost effective approach to start the internal linear combustion engine using its coupled tubular permanent magnet linear generator proposed here. This linear generator operates in this case in motoring mode, providing the required thrust force by feeding this linear generator phases with currents by using a three phase PWM inverter controlled by position feedback scheme. In order to provide the desired thrust force with speciflc value and direction, a position feedback is required to control the free piston engine motion through controlling the inverter switches using PWM control scheme.
Master equation approach to the central spin decoherence problem: Uniform coupling model and role of projection operators (2011)

The generalized master equation of Nakajima-Zwanzig (NZ) type has been used extensively to investigate the coherence dynamics of the central spin model with a nuclear bath in a narrowed state characterized by a well-defined value of the Overhauser field. We reconsider the perturbative NZ approach and apply it to the exactly solvable case of a system with uniform hyperfine couplings. This is motivated by the fact that the effective-Hamiltonian-based theory suggests that the dynamics of the realistic system at low magnetic fields and short times can be mapped onto the uniform coupling model. We show that the standard NZ approach fails to reproduce the exact solution of this model beyond very short times, while the effective-Hamiltonian calculation agrees very well with the exact result on time scales during which most of the coherence is lost. Our key finding is that, in order to extend the time scale of applicability of the NZ approach in this case, instead of using a single projection operator one has to use a set of correlated projection operators which properly reflect the symmetries of the problem and greatly improve the convergence of the theory. This suggests that the correlated projection operators are crucial for a proper description of narrowed-state free-induction decay at short times and low magnetic fields. Our results thus provide important insights toward the development of a more complete theory of central spin decoherence applicable in a broader regime of time scales and magnetic fields.
Changes in Protein Patterns and Translatable Messenger RNA Populations during Cold Acclimation of Alfalfa (1987)

Changes in the rate and pattern of protein synthesis and in translatable mRNA population during cold acclimation of alfalfa (Medicago falcata cv Anik) seedlings have been examined. There appears to be a positive correlation between the increase in ability to synthesize proteins at 4 degrees C and the increase in freezing resistance (survival at -10 degrees C). Results obtained with three different approaches using sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern visualized by (a) staining, (b) immunoblotting and autoradiography, and (c) fluorography of in vivo labeled proteins, show that at least eight polypeptides are newly synthesized during cold acclimation. Results of analysis of in vitro translation products of mRNA from nonacclimated and acclimated seedlings show the appearance of new translatable mRNAs. It is concluded that changes in gene expression occur during cold acclimation, most probably at the transcriptional level.
Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin Wounds (2023)

Microporous annealed particle (MAP) scaffolds are injectable granular materials comprised of micron sized hydrogel particles (microgels). The diameter of these microgels directly determines the size of the interconnected void space between particles where infiltrating or encapsulated cells reside. This tunable porosity allows us to use MAP scaffolds to study the impact of spatial confinement (SC) on both cellular behaviors and the host response to biomaterials. Despite previous studies showing that pore size and SC influence cellular phenotypes, including mitigating the macrophage inflammatory response, there is still a gap in knowledge regarding how SC within a biomaterial modulates immune cell recruitment <i>in vivo</i> in wounds and implants. Thus, we studied the immune cell profile within confined and unconfined biomaterials using small (40 μm), medium (70 μm), and large (130 μm) diameter spherical microgels, respectively. We discovered that MAP scaffolds imparted regenerative wound healing with an IgG1-biased Th2 response. MAP scaffolds generated from 130 μm diameter microgels have a median pore size that can accommodate ∼40 µm diameter spheres induced a more balanced pro-regenerative macrophage response and better wound healing outcomes with more mature collagen regeneration and reduced levels of inflammation.
On integrated radar and communication systems using Oppermann sequences (2008)

In this paper, we consider the design of integrated radar and communication systems that utilize weighted pulse trains with the elements of Oppermann sequences serving as complex-valued weights. An analytical expression of the ambiguity function for weighted pulse trains with Oppermann sequences is derived. Given a family of Oppermann sequences, it is shown that the related ambiguity function depends only on one sequence parameter. This property simplifies the design of the associated weighted pulse trains as it constrains the degrees of freedom. In contrast to the single polyphase pulse compression sequences that are typically deployed in radar applications, the families considered in this paper form sets of sequences. As such, they readily facilitate also multiple-access in communication systems. Numerical examples are provided that show the wide range of options offered by Oppermann sequences in the design of integrated radar and communication systems.
Studies on the synthesis of novel carbohydrates with sulphur in the ring. Part II. Analogues of derivatives of unsaturated deoxy-ulopyranosidonic acids via Diels–Alder reactions with methyl cyanodithioformate (1975)

3,6-Dihydro-2-methylthio-2H-thiopyran-2-carbonitrile (5) and its 3-methoxy-derivative (11) were obtained directly from the Diels–Alder reactions of methyl cyanodithioformate (4) with buta-1,3-diene and trans-1-methoxy-buta-1,3-diene, respectively. The regioselectivity of the reagent (4) is opposite to that of carbonyl dienophiles with 1-alkoxybuta-1,3-dienes. The structure of compound (11) was established by 1H n.m.r. studies involving compounds (5) and (11) and their selectively oxidized and/or reduced derivatives. The results indicated that (11) exists predominantly in the 0H2 conformation in chloroform.
Geometric correction of airborne scanner imagery using orthophotos and triangulated feature point matching (1993)

Abstract We present here a new scheme that performs geometrical correction for airborne multi-spectral scanner imagery provided that an adequate reference image, i.e., an orthophoto, is available. Instead of the ray-tracing method, we determine the corresponding ground coordinates for each pixel of an input image by integrating feature point matching under Delaunay triangulated networks. The major components of the scheme include progressive generation of registration control points, and image registration. Comprehensive investigation for quantitative error analysis is also included. Experimental results indicate that the corrected images attain an accuracy of better than 09 pixels.
FROM MICROMECHANICS TO STRUCTURAL ENGINEERING (1993)

This paper reviews the development of pseudo strain-hardening cement based short fiber composites employing the Performance Driven Design Approach. The micromechanics theory behind the design concept is reviewed, and the unique mechanical properties of the resulting composite are summarized. The translation of material properties to structural properties is demonstrated with the structural response of the Ohno shear beam.
Preparation of poly(ethylene glycol) methacrylate coated CuInS2/ZnS quantum dots and their use in cell staining (2012)

A simple synthesis of poly(ethylene glycol) methacrylate (PEGMA) coated CuInS2/ZnS quantum dots (QDs) has been developed. X-ray diffraction, transmission electron microscopy, and atomic force microscopy observations demonstrated that uniform CuInS2/ZnS QDs were successfully prepared. Fourier transform infrared spectroscopy indicated no new peak after the coating process, indicating that only physical interactions occurred during coating and that PEGMA did not disturb the crystal structure of the CuInS2/ZnS QDs. After PEGMA coating, the photoluminescence spectra of the CuInS2/ZnS QDs red shifted (from 566 nm to 589 nm) and the QD particle size increased. The concentration and molecular weight of PEGMA play important roles in the water solubility and hydrodynamics of the particles. The PEGMA-coated CuInS2/ZnS QDs showed stable emissions for up to 3 weeks. As a demonstration of a potential biomedical application, PEGMA-coated CuInS2-ZnS QDs were used in labeling human liver carcinoma (HepG2) tumor cells.
Force of Single Kinesin Molecules Measured with Optical Tweezers (1993)

Isometric forces generated by single molecules of the mechanochemical enzyme kinesin were measured with a laser-induced, single-beam optical gradient trap, also known as optical tweezers. For the microspheres used in this study, the optical tweezers was spring-like for a radius of 100 nanometers and had a maximum force region at a radius of approximately 150 nanometers. With the use of biotinylated microtubules and special streptavidin-coated latex microspheres as handles, microtubule translocation by single squid kinesin molecules was reversibly stalled. The stalled microtubules escaped optical trapping forces of 1.9 +/- 0.4 piconewtons. The ability to measure force parameters of single macromolecules now allows direct testing of molecular models for contractility.
Chemical Offense by Means of Toxicysts in the Freshwater Ciliate, Coleps hirtus (2014)

Coleps hirtus is a small common freshwater ciliate belonging to the protostomatid group, its body covered by calcified plates assembled to form an armor. Coleps feeds on bacteria, algae, flagellates, living and dead ciliates, animal and plant tissues. To assist its carnivorous feeding the ciliate is equipped with offensive extrusomes (toxicysts), clustering mainly in and around its oral aperture. In this study, we isolated the discharge of the toxicysts from living cells, evaluating its cytotoxic effects against various ciliate species, and demonstrating that it is essential for the effectiveness of Coleps' predatory behavior. The analysis of the toxicyst discharge performed by liquid chromatography-electrospray-mass spectrometry and gas chromatography-mass spectrometry, revealed the presence of a mixture of 19 saturated, monounsaturated and polyunsaturated free fatty acids with the addition of a minor amount of a diterpenoid (phytanic acid).
Modeling Fully Coupled Oil–Gas Flow in a Dual-Porosity Medium (2005)

A finite element model has been developed to simulate two-phase, (i.e., oil and gas) flow and solid deformation in a dual-porosity medium. The model accounts for coupling between solid deformations and fluid flow in both the primary medium (representing the matrix pores and solid) and the secondary medium (used to represent fractures in the present study). The model is verified against relevant analytical solutions and then applied to the problem of an inclined wellbore under generalized plane strain conditions, subjected to a three-dimensional in situ state of stress in a fractured formation saturated with oil and gas. A parametric study has been carried out to demonstrate the effect of dual-porosity parameters, phase saturations, and interaction between the two media. The implementation of double effective stress laws in the present study is a significant deviation from some classical dual-porosity models and helps to incorporate the effect of deformation of the secondary medium (representing the fractures).
Improvement of a Vortex Shedding Flowmeter Performance by Placing a Narrow Gap around a Bluff Body Base (1999)

By placing a gap around the bases of the bluff body, a linearity of a vortex shedding flowmeter has been significantly improved. Measurement error caused by an upstream pipe fitting has also been remarkably reduced.
Sparse correlation matching-based spectrum sensing for open spectrum communications (2012)

To deal with the current spectrum scarcity problem and exploiting the fact that exclusive access through tightly regulated licensing leads to idle spectrum, cognitive radio has been proposed as a way to reuse this underutilized spectrum in an opportunistic manner, i.e., allowing the use of temporarily unused licensed spectrum to secondary users who have no spectrum licenses. To protect the licensed users from the cognitive users' interference, the opportunistic user requires knowledge of the original license holder activity. In this article, a feature-based approach for spectrum sensing based on periodic non-uniform sampling is addressed. In particular, we face the compressed-sampling version of detecting predetermined spectral shapes in sparse wideband regimes by means of a correlation-matching procedure.
Use of an Alternative Light Source to Assess Strangulation Victims (2013)

Alternative light sources (ALSs) are commonly used at crime scenes and in forensic laboratories to collect evidence such as latent fingerprints, body fluids, hair, and fibers. This article describes the use of this technology to reveal soft tissue injuries that are not visible to the naked or unaided eye in patients who report strangulation. The value of this information to the medical, nursing, and judicial systems is discussed. The records of the 172 strangulation patients seen in our forensic nurse examiner program between 2009 and 2010 were reviewed. The SPEX Crimescope (SPEX Forensics, Edison, New Jersey) was used during the assessment of all of them. Ninety-three percent of the patients had no visible evidence of external injuries on physical examination. The ALS revealed positive findings of intradermal injuries in 98% of that group. Information obtained with ALS devices helps medical and nursing practitioners understand the gravity of patients' injuries, influences medical treatment decisions and follow-up care, and supports the prosecution of the perpetrators of crimes of violence. Educational programs about the application of ALS and the interpretation of its findings are valuable for medical, nursing, and other forensic disciplines.
Nucleotide Sequence of the Gene Encoding Pepstatin-insensitive Acid Protease B, Scytalidopepsin B, ofScytalidium lignicolum (1996)

A chromosomal DNA fragment of Scytalidium lignicolum that encodes the mature enzyme region of acid protease B (Scytalidopepsin B), was cloned and its nucleotides sequenced. The fragment contained a 76-bp intron at the middle of the mature enzyme-coding region. The mature enzyme was composed of 206 amino acid residues with a molecular weight of 21,550. There were some discrepancies between the amino acid sequence deduced from these results and that previously established by protein sequencing.