gebhart/patrepeval huggingface dataset:
Tailoring surface properties of liposomes for dexamethasone intraocular administration (2023)

Diseases of the posterior eye segment are often characterized by intraocular inflammation, which causes, in the long term, severe impairment of eye functions and, ultimately, vision loss. Aimed at enhancing the delivery of anti-inflammatory drugs to the posterior eye segment upon intravitreal administration, we developed liposomes with an engineered surface to control their diffusivity in the vitreous and retina association. Hydrogenated soybean phosphatidylcholine (HSPC)/cholesterol liposomes were coated with (agmatinyl)<sub>6</sub>-maltotriosyl-acetamido-N-(octadec-9-en-1-yl)hexanamide (Agm<sub>6</sub>-M-Oleate), a synthetic non-peptidic cell penetration enhancer (CPE), and/or 5% of mPEG<sub>2kDa</sub>-DSPE. The zeta potential of liposomes increased, and the mobility in bovine vitreous and colloidal stability decreased with the Agm<sub>6</sub>-M-Oleate coating concentration. Oppositely, mPEG<sub>2kDa</sub>-DSPE decreased the zeta potential of liposomes and restored both the diffusivity and the stability in vitreous. Liposomes with 5 mol% Agm<sub>6</sub>-M-Oleate coating were well tolerated by ARPE-19 retina cells either with or without mPEG<sub>2kDa</sub>-DSPE, while 10 mol% Agm<sub>6</sub>-M-Oleate showed cytotoxicity. Agm<sub>6</sub>-M-Oleate promoted the association of liposomes to ARPE-19 cells with respect to plain liposomes, while mPEG<sub>2kDa</sub>-DSPE slightly reduced the cell interaction. Dexamethasone hemisuccinate (DH) was remotely loaded into liposomes with a loading capacity of ∼10 wt/wt%. Interestingly, mPEG<sub>2kDa</sub>-DSPE coating reduced the rate of DH release and enhanced the disposition of Agm<sub>6</sub>-M-Oleate coated liposomes in the ARPE-19 cell cytosol resulting in a more efficient anti-inflammatory effect. Finally, mPEG<sub>2kDa</sub>-DSPE enhanced the association of DH-loaded Agm<sub>6</sub>-M-Oleate coated liposomes to explanted rat retina, which reflected in higher viability of inner and outer nuclear layer cells.
Factorization of Block Triangular Matrix Functions with Off-diagonal Binomials (2005)

Factorizations of Wiener-Hopf type are considered in the abstract framework of Wiener algebras of matrix-valued functions on connected compact abelian groups, with a non-archimedean linear order on the dual group. A criterion for factorizability is established for 2 × 2 block triangular matrix functions with elementary functions on the main diagonal and a binomial expression in the off-diagonal block.
Structure of a novel twist-boat spiroketal (1992)

C 29 H 40 O 5 Si cristallise dans C2/c avec a = 38,29, b = 7,774 et c = 20,65 A, β = 109,05 °, Z = 8; affinement jusqu'a R = 0,075. Un cycle tetrahydropyrane est dans une conformation chaise, l'autre dans une conformation plus inhabituelle bateau tordu.
Reflection ellipsometry for in-situ measurements of complex permittivity and thickness of a single-layer material at microwave frequencies : theory and experiments (2002)

For in-situ measurement of the complex permittivity of planar materials, we have developed a free-space experimental setup based on reflection ellipsometry and extended to microwave frequencies. Different angles of incidence were studied in the range [35 ; 50°]. Original numerical methods, derived from analytical relations based on contour line charts and least-square optimization, allow to highlight the influence of measurement uncertainties. Novel developments concerning the simultaneous determination of both complex permittivity and thickness of a single-layer sample are shown.
Corpus Luteum Function and Adenylyl Cyclase Stimulability in the Rat after an Estradiol Benzoate-Induced Ovulatory Surge of Luteinizing Hormone: Role of Prolactin* (1980)

Estradiol benzoate (E; 20 μg, sc) was given on diestrus day 1 to 4-day cycling rats to advance the ovulatory LH surge by 1 day. Estrogen treatment is known to result in the maintenance of two apparently functional sets of corpora lutea (CL). Previously, our laboratory has demonstrated that E treatment results in the maintenance of LH-stimulable adenylyl cyclase activity in the 4-day-old CL on the day of expected proestrus. We investigated whether the effect of E was mediated by PRL by using an eryocryptine analog CB-154 to inhibit PRL release. We measured LH- and isoproterenol (Iso)-stimulable adenylyl cyclase activity and progesterone levels in 4- and 1-day-old CL and progesterone levels in serum. Iso was used as a control hormone to monitor receptor-mediated regulation of adenylyl cyclase activity. Progesterone was measured by RIA to examine whether LH-stimulable adenylyl cyclase activity correlated with CL function. We found that E treatment resulted in increased LH-stimulable adenylyl cyclase activity and elevated luteal progesterone in the 4-day-old CL when compared to control CL; serum progesterone was also elevated after E treatment. CB-154 plus E treatment prevented the elevation of tissue and serum progesterone and prevented the increase in LH stimulability of adenylyl cyclase activity in 4-day-old CL. PRL plus CB-154 plus E reversed the effect of CB-154 plus E and resulted in increased LH stimulability of CL cyclase and in elevated tissue and serum progesterone. LH-stimulable adenylyl cyclase activity and tissue progesterone content were low in all 1-day-old CL. Interestingly, Iso-stimulable adenylyl cyclase activity was high (8- to 10-fold) in all 4-day-old CL. We confirm that E treatment 1) advanced the LH surge and ovulation by 1 day, 2) resulted in pseudopregnancy, and 3) maintained LH-stimulable adenylyl cyclase activity after a desensitizing LH surge. We conclude that PRL is part of the means by which estrogen acts to maintain LH-stimulable adenylyl cyclase activity and functionality in 4-day-old CL. (Endocrinology106: 382, 1980)
Evaluation d'un système multirobot. Cas d'une constellation de satellites (2009)

Multisatellite systems are particular multiagent systems in so far as adding a satellite agent into the system is very costly. Therefore evaluating a multisatellite system through simulations is a major issue. However it is of great importance to set relevant measures so as to be able to make a trade-off between the eficiency and the size of the system. A pratical case is presented in this paper: the evaluation of decentralized coordination algorithms for a constellation of autonomous observation satellites. An experiment notion and metrics concerning performance, stability and scalability are defined and the corresponding results are discussed.
Lactosylceramide is synthesized in the lumen of the Golgi apparatus (1994)

Recently, synthesis of lactosylceramide has been described to occur on the cytosolic face of the Golgi [(1991) J. Biol. Chem. 266, 20907-20912]. The reactions following in the biosynthesis of higher glycosphingolipids are known to take place in the lumen of the Golgi. For our understanding of the functional organization of the multiglycosyltransferase system of glycosphingolipid synthesis in the Golgi, the knowledge of the topology of individual reactions is a prerequisite. We have developed a simple and quick assay system for sphingolipid biosynthesis and have obtained evidence that lactosylceramide is synthesized in the lumen of the Golgi. Because lactosylceramide is generated by galactosylation of glucosylceramide which, in turn, is synthesized from ceramide and UDP-Glc on the cytosolic surface of the Golgi apparatus, further efforts will be directed to the characterization of a glucosylceramide-translator in the Golgi membranes rather than a lactosylceramide-translocator.
A Semi-Automated Method for Monitoring Dam Passage of Upstream Migrant Yellow-Phase American Eels (2014)

Abstract Fish passage facilities at dams have become an important focus of fishery management in riverine systems. Given the personnel and travel costs associated with physical monitoring programs, automated or semi-automated systems are an attractive alternative for monitoring fish passage facilities. We designed and tested a semi-automated system for eel ladder monitoring at Millville Dam on the lower Shenandoah River, West Virginia. A motion-activated eel ladder camera (ELC) photographed each yellow-phase American Eel Anguilla rostrata that passed through the ladder. Digital images (with date and time stamps) of American Eels allowed for total daily counts and measurements of eel TL using photogrammetric methods with digital imaging software. We compared physical counts of American Eels with camera-based counts; TLs obtained with a measuring board were compared with TLs derived from photogrammetric methods. Data from the ELC were consistent with data obtained by physical methods, thus supporting the semi-automated camera system as a viable option for monitoring American Eel passage. Time stamps on digital images allowed for the documentation of eel passage time—data that were not obtainable from physical monitoring efforts. The ELC has application to eel ladder facilities but can also be used to monitor dam passage of other taxa, such as crayfishes, lampreys, and water snakes. Received December 31, 2013; accepted March 11, 2014
TH‐EF‐207A‐02: Imaging Pancreatic Î2‐Cell Function with 51/52Mn‐PET (2016)

Purpose: To image β‐cells noninvasively using radio‐manganese PET and to develop efficient small cyclotron production of 51 Mn (t1/2=46m, β + =97%) and 52 Mn (t1/2=5.6d, β + =29%). Methods: 51 Mn and 52 Mn were produced by 16 MeV proton irradiation (GE PETtrace) of electrodeposited 54 Fe on silver and Cr metal pressed into a silver disc, respectively. 51 Mn was radiochemically isolated from target material by anion exchange chromatography and 52 Mn was isolated by ethanolic anion exchange trap‐and‐release. A final injectable product of 51 Mn 2+ or 52 Mn 2+ was obtained in 0.01M pH 6.0 NaOAc. To assess pancreatic uptake, fasted ICR mice were administered and intravenous bolus or infusion of 52 Mn 2+ . Additionally, to demonstrate the correlation between β‐cell function and 52 Mn 2+ pancreatic uptake, prior to tracer administration groups of ICR mice were administered glibenclamide (5mg/kg) and diazoxide (20 mg/kg) as an insulin release stimulator and blocker, respectively. To validate PET ROI quantification, ex vivo biodistribtution studies were conducted on each subject after the final imaging time‐point. Results: Dynamic PET data using a left atrium ROI revealed that 52 Mn 2+ cleared from the blood with a 10 second half‐life. Significant uptake was seen in the pancreas (approximately 20% ID/g, SUVmean= 5.5), liver, kidneys, intestine, heart, and thyroid. Pancreatic uptake was found to be highly sensitive to volatile anesthesia administration (p=0.0002), insulin release stimulation by glibenclamide (p=0.017), and by insulin release inhibition by diazoxide (p=0.046). Excellent agreement was found between in vivo PET ROI quantification and ex vivo biodistribution measurements. Conclusion: This work demonstrates the feasibility of using radiomanganese‐PET for measuring functional β‐cell mass in vivo. The decay characteristics and dosimetric properties of 51 Mn are well suited for clinical PET, which will allow for rapid translation and application.
QS45. Parallels And Deviations in Mesenchymal Progenitor Cell Activation in Regenerative and Fibrotic Injuries (2022)

Purpose: The formation of ectopic bone during heterotopic ossification (HO) and formation of new tissues after digit tip amputation (DTA) have been shown to progress through a Pdgfra+ mesenchymal progenitor cell (MPC). Both processes result in the formation of de novo bone at the injury site. Here, we sought to compare gene expression and differentiation trajectories of the MPCs in HO formation and DTA repair in order to identify similarities and differences in these processes that may be beneficial for future wound regeneration strategies. Methods: Single cell RNA sequencing (scRNAseq) datasets from multiple time points in the burn/tenotomy model of achilles tendon HO, amputation of the P3 digit (regenerative) and P2 digit (fibrotic) were analyzed. MPCs were identified using known markers (Pdgfra, Prrx1), subsetted and re-analyzed for more in-depth analysis. Computational assessments were carried out using the R packages Seurat and Monocle2. Results: Our analyses point to a common response to injury, including expansion of the progenitor pool, deposition and interaction with rebuilding matrix components, and regulation of major morphogen pathways such as TGFβ and WNT within Pdgfra+ MPCs. However, these common injury responses were regulated via unique mechanisms. Surprisingly, MPCs of the non-fibrotic P2 DTA showed more transcriptional similarity with injury-induced tendon HO than with the MPCs present within the blastema of the regenerative P3 DTA. In addition to divergent regulations of the TGFβ and WNT pathways, analyses show a unique fingerprint of inflammatory signaling activation within the MPCs of P2 and P3 DTA, as well as HO. This included preferential activation of the IL1 and IL6/10 signaling in regenerative P3 DTA, Toll-like receptor signaling in the non-regenerative P2 DTA, NFκB in both DTA models and the classical complement pathway in HO. Conclusion: Combined, this data suggests that while similar mechanisms are employed to overcome injury, these pathways are alternatively regulated to meet their individual injury requirements. Modulating these pathways may prove valuable tools to shift the balance between regenerative and fibrotic healing response.
Inhibition of Glycosphingolipid Biosynthesis:  Application to Lysosomal Storage Disorders (2000)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInhibition of Glycosphingolipid Biosynthesis: Application to Lysosomal Storage DisordersTerry D. Butters, Raymond A. Dwek, and Frances M. PlattView Author Information Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K. Cite this: Chem. Rev. 2000, 100, 12, 4683–4696Publication Date (Web):November 3, 2000Publication History Received21 March 2000Published online3 November 2000Published inissue 1 December 2000https://pubs.acs.org/doi/10.1021/cr990292qhttps://doi.org/10.1021/cr990292qresearch-articleACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1596Altmetric-Citations261LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Carbohydrates,Inhibition,Inhibitors,Peptides and proteins,Rodent models Get e-Alerts
Regional-Scale Monitoring of Rice Flood Disaster Based on Multi-Temporal Remote Sensing Images (2018)

Rice flood disaster causes wide range of damage in the rice production worldwide. To obtain the spatial distribution of damage area and access the damage severity, a fast and accurate loss assessment method is of great importance for effective administration. The objective of this study was to determine suitable spectral features for rice flood disaster detecting and to develop a mapping method at regional scale based on satellite remote sensing image data. Within a number of vegetation indices (VIs) that were examined for their sensitivity to the rice flood disaster, Normalized Difference Water Index (NDWI) and enhanced vegetation index (EVI) were identified as the optimal VIs for detecting rice flood disaster. Based on the two-date satellite images and multi-temporal analysis, a mapping method for monitoring the rice flood disaster at a regional scale were developed based on support vector machine (SVM) and maximum likelihood classifier (MLC). And the model that relies on SVM method significantly outperformed the MLC method, with the overall accuracy increased from 0.64 to 0.82. The successful application of this method in a typical rice flood disaster in Anhui Province, China demonstrated the feasibility of the method and showed its promising potential to be implemented in practice.
Construction and Application of Membrane-Bound Angiotensin-I Converting Enzyme System: A New Approach for the Evaluation of Angiotensin-I Converting Enzyme Inhibitory Peptides (2020)

The effect of the plasma membrane on the activity of angiotensin-I converting enzyme (ACE) plays a crucial role in the evaluation of food-derived ACE inhibitory peptides, although these peptides are commonly evaluated in the system with ACE in its free state. In this study, we constructed an in vitro membrane-bound ACE C domain system to simulate the presence of the plasma membrane. The resultant <i>K</i><sub>m</sub> and <i>V</i><sub>max</sub> suggested that the presence of the membrane reduced the affinity between ACE C domain and hippuryl-histidyl-leucine, while it increased the reaction velocity. The ACE inhibitory activity of four egg white peptides and five structurally modified peptides suggested that a moderate hydrophobicity/hydrophilicity of the peptide is beneficial for the improvement of their ACE inhibitory activity in a membrane-bound system. These results also indicated that the N terminal plays a significant role in the ACE inhibitory activity of peptides in the membrane-bound system.
Venue2Vec: An Efficient Embedding Model for Fine-Grained User Location Prediction in Geo-Social Networks (2019)

Geo-Social Networks (GSN) significantly improve location-aware capability of services by offering geo-located content based on the huge volumes of data generated in the GSN. The problem of user location prediction based on user-generated data in GSN has been extensively studied. However, existing studies are either concerning predicting users’ next check-in location or predicting their future check-in location at a given time with coarse granularity. A unified model that can predict both scenarios with fine granularity is quite rare. Also, due to the heterogeneity of multiple factors associated with both locations and users, how to efficiently incorporate these information still remains challenging. Inspired by the recent success of word embedding in natural language processing, in this paper, we propose a novel embedding model called Venue2Vec which automatically incorporates temporal-spatial context, semantic information, and sequential relations for fine-grained user location prediction. Locations of the same type, and those that are geographically close or often visited successively by users will be situated closer within the embedding space. Based on our proposed Venue2Vec model, we design techniques that allow for predicting a user’s next check-in location, and also their future check-in location at a given time. We conduct experiments on three real-world GSN datasets to verify the performance of the proposed model. Experimental results on both tasks show that Venue2Vec model outperforms several state-of-the-art models on various evaluation metrics. Furthermore, we show how the Venue2Vec model can be more time-efficient due to being parallelizable.
How to Predict Conformations Accessible to a Molecule in Solution: Validation of a Force Field-Based Prediction of NOE Distances by Comparison with the Experimental Data for the Series of Compounds CH3C[CH2P(Bzl)R]3Mo(CO)3 (R = Ph,m-Xyl) (1998)

NMR-NOE analysis of the three compounds (RRS/SSR)-CH3C(CH2PPhBz)3Mo(CO)3 (1), (RRR/SSS)-CH3C(CH2PPhBz)3Mo(CO)3 (2), and (RRR/SSS)-CH3C[CH2P(m-Xyl)Bz]3Mo(CO)3 (3), leads to experimental values for a number of intramolecular H···H contacts in each case. By the very nature of the NOE method, and with the type of molecules studied here, these values have to be understood as a Boltzmann weighted average over all the conformations accessible to the molecules in solution. – A Boltzmann weighted force field approach is used to predict these values on the basis of sets of force field parameters derived earlier for this class of compounds. The agreement between observed and calculated NOE distances is highly satisfactory in each case (rms = 0.2 Å to 0.3 Å). By a statistical analysis it is shown that the predictions made by applying this Boltzmann weighted force field approach are highly significant. There is only a chance of 3 in 100 000 that this quality of prediction might be obtained by chance. This is taken as a validation, albeit indirect, of the energy scale reproduced by the force field parameters as developed. The methods described give a detailed insight into the conformational flexibility of molecules of the type studied. Solid state structures of the molecules are also correctly modelled by the force field used.
In vitro anticomplementary activity of phenylpropanoids from Agastache rugosa (1996)

In searching for anticomplementary compounds, three phenylpropanoids were isolated from the roots of Agastache rugosa and identified as rosmarinic acid (RA), rosmarinic acid methyl ester (RAM) and caffeic acid methyl ester (CAM) by NMR analyses. RA and RAM exhibited strong inhibitory activity on both the classical pathway (CP) and the alternative pathway (AP) of the complement system, in vitro, but CAM did far less than RA and RAM. RAM-MI~-M5, the methylated derivatives from the RAM, showed that the inhibitory activity was decreased in inverse proportion to the number of methylated groups and RAM-M 2~-M4, the isomers of two methylated hydroxyl groups, exhibited different inhibition activity.
Utilizing AgCl:Ag and AgCl mesostructures as solid precursors in the formation of highly textured silver nanomaterials via electron-beam induced decomposition (2012)

Herein, we report on the facile synthesis of AgCl:Ag and AgCl mesocubes with edge lengths up to 500 nm. When these mesostructures were placed under an electron beam, localized heating resulted in the decomposition of the AgCl regions of the respective materials leading to the formation of highly textured silver nanostructures. Furthermore, we utilized the AgCl:Ag and AgCl mesocubes along with their respective silver analogs for use in surface enhanced Raman spectroscopy (SERS) applications.
Interactions between a Plasma-Activated Medium and Cancer Cells (2016)

A plasma-activated medium (PAM) is a medium that is irradiated with plasma; PAMs have garnered much attention from the plasma medicine community. PAMs induce apoptosis in many types of cancer cells and have a clinical benefit, especially in disseminated cancers such as peritoneal disseminated ovarian cancer and gastric cancer. Although the effectiveness of PAMs has been extensively reported, the mechanisms of the selective killing of cancer cells by PAMs still remain to be elucidated. Systems biology is a key to understanding the origins of the differences in cellular sensitivities to PAM.