gebhart/patrepeval huggingface dataset:
Systemic IL-27 administration prevents abscess formation and osteolysis via local neutrophil recruitment and activation (2022)

Interleukin-27 is a pleiotropic cytokine whose functions during bacterial infections remain controversial, and its role in patients with S. aureus osteomyelitis is unknown. To address this knowledge gap, we completed a clinical study and observed elevated serum IL-27 levels (20-fold higher, P < 0.05) in patients compared with healthy controls. Remarkably, IL-27 serum levels were 60-fold higher in patients immediately following septic death than in uninfected patients (P < 0.05), suggesting a pathogenic role of IL-27. To test this hypothesis, we evaluated S. aureus osteomyelitis in WT and IL-27Rα<sup>-/-</sup> mice with and without exogenous IL-27 induction by intramuscular injection of rAAV-IL-27p28 or rAAV-GFP, respectively. We found that IL-27 was induced at the surgical site within 1 day of S. aureus infection of bone and was expressed by M0, M1 and M2 macrophages and osteoblasts but not by osteoclasts. Unexpectedly, exogenous IL-27p28 (~2 ng·mL<sup>-1</sup> in serum) delivery ameliorated soft tissue abscesses and peri-implant bone loss during infection, accompanied by enhanced local IL-27 expression, significant accumulation of RORγt<sup>+</sup> neutrophils at the infection site, a decrease in RANK<sup>+</sup> cells, and compromised osteoclast formation. These effects were not observed in IL-27Rα<sup>-/-</sup> mice compared with WT mice, suggesting that IL-27 is dispensable for immunity but mediates redundant immune and bone cell functions during infection. In vitro studies and bulk RNA-seq of infected tibiae showed that IL-27 increased nos1, nos2, il17a, il17f, and rorc expression but did not directly stimulate chemotaxis. Collectively, these results identify a novel phenomenon of IL-27 expression by osteoblasts immediately following S. aureus infection of bone and suggest a protective role of systemic IL-27 in osteomyelitis.
Supermicroporous Silica Nanograins: Synthesis and Application (2019)

Supermicroporous silicas having micropores 1-2 nm in diameter were miniaturized to 30-40 nm in diameter using a combination of binary organic agents: cationic surfactant as a porogen and polyethylene glycol as a growth suppressor in a concentrated system. The miniaturized nanograins of the porous silica were effective as a porous and transparent matrix for WO<sub>3</sub> quantum dots that exhibit enhanced photocatalytic activity.
Fatal enhanced respiratory syncytial virus disease in toddlers (2021)

In 1967, two toddlers immunized with a formalin-inactivated vaccine against respiratory syncytial virus (FIRSV) in the United States died from enhanced RSV disease (ERD), a severe form of illness resulting from aberrant priming of the antiviral immune response during vaccination. Up to 80% of immunized children subsequently exposed to wild-type virus were hospitalized. These events hampered RSV vaccine development for decades. Here, we provide a characterization of the clinical, immunopathological, and transcriptional signature of fatal human ERD, outlining evidence for safety evaluation of RSV vaccines and a framework for understanding disease enhancement for pathogens in general.
Assessing the Absorption of New Pharmaceuticals (2001)

The advent of more efficient methods to synthesize and screen new chemical compounds is increasing the number of chemical leads identified in the drug discovery phase. Compounds with good biological activity may fail to become drugs due to insufficient oral absorption. Selection of drug development candidates with adequate absorption characteristics should increase the probability of success in the development phase. To assess the absorption potential of new chemical entities numerous in vitro and in vivo model systems have been used. Many laboratories rely on cell culture models of intestinal permeability such as, Caco-2, HT-29 and MDCK. To attempt to increase the throughput of permeability measurements, several physicochemical methods such as, immobilized artificial membrane (IAM) columns and parallel artificial membrane permeation assay (PAMPA) have been used. More recently, much attention has been given to the development of computational methods to predict drug absorption. However, it is clear that no single method will sufficient for studying drug absorption, but most likely a combination of systems will be needed. Higher throughput, less reliable methods could be used to discover 'loser' compounds, whereas lower throughput, more accurate methods could be used to optimize the absorption properties of lead compounds. Finally, accurate methods are needed to understand absorption mechanisms (efflux-limited absorption, carrier-mediated, intestinal metabolism) that may limit intestinal drug absorption. This information could be extremely valuable to medicinal chemists in the selection of favorable chemo-types. This review describes different techniques used for evaluating drug absorption and indicates their advantages and disadvantages.
Comparative histochemical, biochemical and immunocytochemical studies of cathepsin B in human gingival (1994)

Cathepsin B activity was demonstrated histochemically in unfixed cryostat sections of inflamed human gingiva using the 2-methoxy-4-naphthylamide (MNA) substrates Z-Val-Lys-Lys-Arg-MNA and Z-Ala-Arg-Arg-MNA with a post-azo-coupling technique. Enzyme localisation was confirmed by immunocytochemistry with polyclonal sheep anti-human cathepsin B. In both cases, staining was found in connective tissue fibroblasts and also in cells varying in shape from rounded to more irregular forms. The latter were present both in areas of cellular infiltration and in the oral and pocket epithelium. Examination of adjacent sections with monoclonal antibodies directed against leukocyte differentiation antigens showed that the rounded to irregular cells were CD68 positive macrophages and monocytes. The histochemical staining had the form of fine cytoplasmic particles consistent with the known lysosomal occurrence of cathepsin B. Cells stained by the post-coupling method using the tryptase substrates Z-Ala-Ala-Lys-MNA and D-Val-Leu-Arg-MNA showed a different distribution and morphology, with reaction product confined to mast cell granules. The differences between the cathepsin B and tryptase staining patterns were confirmed by differential extraction from cryostat sections with salt-free and high-salt buffers respectively. Biochemical characterisation of activities in the extracts with the 7-amino-4-trifluoromethyl coumarin (AFC) substrates Z-Val-Lys-Lys-Arg-AFC and Z-Ala-Ala-Lys-AFC and protease inhibitors confirmed the identity of the two enzymes. Selective inhibitors could also be used in histochemical incubations to distinguish between cathepsin B and tryptase staining.
The role of the renin-angiotensin-aldosterone system in cardiovascular homeostasis in normal human subjects. (1976)

To examine the role of angiotensin II in the maintenance of blood pressure and the control of aldosterone secretion in man, eight normal subjects were studied on a tilt table in sodium replete and sodium depleted states prior to and subsequent to the intravenous infusion of an angiotensin converting enzyme inhibitor (CEI). In both the sodium replete or sodium depleted state, upright tilting resulted in an increase in heart rate and a narrowing of pulse pressure. None of the sodium replete or depleted subjects fainted. Tilting was accompanied by a rise in plasma renin activity with an associated rise in plasma aldosterone concentration. When converting enzyme inhibitor was administered, which blocked the generation of angiotensin II, sodium replete subjects were able to compensate for an upright tilt, despite the absence of angiotensin II, without significant hemodynamic change when compared to control state. In sodium depleted subjects, after the administration of converting enzyme inhibitor, there was a sharp and significant decrease in systolic and diastolic blood pressure associated with a significant rise in heart rate. All but one sodium depleted subject fainted within seven minutes. Both plasma aldosterone concentration and plasma renin activity rose on tilting in both sodium replete and sodium depleted subjects. After the administration of converting enzyme inhibitor, plasma aldosterone failed to rise in association with a rise in plasma renin activity. In supine subjects, after the administration of converting enzyme inhibitor, plasma renin activity rose but plasma aldosterone concentration fell. In sodium depleted subjects, after the administration of CEI, aldosterone fell to a level significantly lower than that in supine controls and to a level no different from the supine sodium replete subject. These results indicate that angiotensin II is essential for blood pressure maintenance in sodium depleted individuals, that angiotensin II exerts a direct feedback control on renin secretion, and that angiotensin II is the primary stimulus to aldosterone secretion in response to both sodium depletion and to posture.
ChemInform Abstract: Hybrid One‐Dimensional 15‐Crown‐5‐ether‐uranyl‐selenate Polymers in [K@(C10H20O5)] [(UO2)(SeO4)(HSeO4)(H2O)]: Synthesis and Characterization. (2015)

Abstract Yellow‐green prismatic crystals of the title compound are obtained from an aqueous solution containing UO 2 (NO 3 ) 2 , K 2 C 2 O 4 , H 2 SeO 4 , and 15‐crown‐5 by evaporation at room temperature.
Two-Step Generation of Oligodendrocyte Progenitor Cells From Mouse Fibroblasts for Spinal Cord Injury (2018)

Oligodendrocyte progenitor cells (OPCs) are attracting attention as the ideal cell therapy for spinal cord injury (SCI). Recently, advanced reprogramming and differentiation techniques have made it possible to generate therapeutic cells for treating SCI. In the present study, we used directly-induced neural stem cells (DNSCs) from fibroblasts to establish OPCs (DN-OPCs) capable of proliferation and confirmed their OPC-specific characteristics. Also, we evaluated the effect of transplanted DN-OPCs on SCI in rats. The DN-OPCs exhibited an OPC-specific phenotype and electrophysiological function and could be differentiated into oligodendrocytes. In the SCI model, transplanted DN-OPCs improved behavior recovery, and showed engraftment into the host spinal cord with expression of myelin basic protein. These results suggest that DN-OPCs could be a new source of potentially useful cells for treating SCI.
Automated microfluidic chromatin immunoprecipitation from 2,000 cells (2009)

Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, cumbersome and require a large number of cells, making it difficult to study rare cell types such as certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000-500,000 cells. This technology may provide a solution to the need for a high sensitivity, rapid, and automated ChIP assay, and in doing so facilitate the use of ChIP for many interesting and valuable applications.
Biomechanical Analysis of Facet and Graft Loading in a Smith-Robinson Type Cervical Spine Model (1994)

In spondylotic specimens (4-5 mm disc heights) disc space distraction in excess of 3.0 mm from preoperative height caused a significant decrease in both the ratio of posterior element to graft loading and posterior element loads. These findings may help explain recent clinical reports of a limit of effective disc space distraction.
Phenotypic characterization of transgenic mice harboring Nf1+/− or Nf1−/− osteoclasts in otherwise Nf1+/+ background (2012)

Skeletal abnormalities in neurofibromatosis type 1 syndrome (NF1) are observed in ∼50% of patients. Here, we describe the phenotype of Nf1(Ocl) mouse model with Nf1-deficient osteoclasts. Nf1Ocl mice with Nf1+/- or Nf1-/- osteoclasts in otherwise Nf1+/+ background were successfully generated by mating parental Nf1flox/flox and TRAP-Cre mice. Contrary to our original hypothesis, osteoporotic or fragile bone phenotype was not observed. The µCT analysis revealed that tibial bone marrow cavity, trabecular tissue volume, and the perimeter of cortical bone were smaller in Nf1 Ocl-/- mice compared to Nf1 Ocl+/+ control mice. Nf1 Ocl-/- mice also a displayed narrowed growth plate in the proximal tibia. In vitro analysis showed increased bone resorption capacity and cytoskeletal changes including irregular cell shape and abnormal actin ring formation in Nf1-/- osteoclasts. Surprisingly, the size of spleen in Nf1 Ocl-/- mice was two times larger than in controls and histomorphometric analysis showed splenic megakaryocytosis. In summary, Nf1Ocl mouse model presented with a mild but specific bone phenotype. This study shows that NF1-deficiency in osteoclasts may have a role in the development of NF1-related skeletal abnormalities, but Nf1-deficiency in osteoclasts in Nf1+/+ background is not sufficient to induce skeletal abnormalities analogous to those observed in patients with NF1.
Physical activity and colon cancer: confounding or interaction? (2002)

Physical activity plays an important role in the etiology of colon cancer. Its significance is seen by its consistent association as an independent predictor of colon cancer as well as by its impact on the odds ratios associated with other factors. Given these observations, it is most probable that physical activity operates through multiple biological mechanisms that influence the carcinogenic process.
Polymerizations with contribution of covalent and ionic species (1995)

Abstract The similarities and dissimilarities between the ring‐opening and vinyl cationic polymerizations are discussed for systems with simultaneous presence of covalent and ionic species. The common denominator in comparing kinetics of polymerization of these systems is the comparison of the total times of polymerization with the time elapsing when a given macromolecule is active (i.e. ionic) and inactive (e.g. covalent in cationic vinyl and ring‐opening polymerization). In the cationic ring‐opening polymerization the reactivation of covalent species proceeds mostly by intramolecular nucleophilic dipole‐dipole reaction (conformationally assisted) involving the chain unit adjacent to growing ester chain end. This reaction is fast (e.g. in THF polymerization) and is responsible for covalent ion‐pair interconversion. In the cationic vinyl polymerization this path does not exist but covalent species (esters) can be faster ionized by using of the nucleophile, increasing thus the rate of exchange between esters and ions. This process provides one of the possible ways for converting the non‐living polymerization into the living‐dormant process. The cationic polymerizations are then compared with covalent (pseudoanionic) polymerization of cyclic esters, initiated with covalent metal alkoxides like R 2 AlOR' or Al(OR) 3 .
Dynamique de l’organisation des triades (2016)

Julie Brocard Oriana Sarrault Didier Grunwald Isabelle Marty Cellular Myology and Pathologies, Grenoble Institute of Neurosciences, Inserm U1216, Grenoble, France Eric Denarier Physiopathology of the Cytoskeleton, Grenoble Institute of Neurosciences, Inserm U1216, Grenoble, France Julien Faure Cellular Myology and Pathologies, Grenoble Institute of Neurosciences, Inserm U1216, Grenoble, France CHU Grenoble, Biochemistry and Molecular Genetics, Grenoble, France 13 JOURNEES DE LA SOCIETE FRANCAISE DE MYOLOGIE Communication orale
Experimental studies of high-confinement mode plasma response to non-axisymmetric magnetic perturbations in ASDEX Upgrade (2016)

Here, the interaction of externally applied small non-axisymmetric magnetic perturbations (MP) with tokamak high-confinement mode (H-mode) plasmas is reviewed and illustrated by recent experiments in ASDEX Upgrade. The plasma response to the vacuum MP field is amplified by stable ideal kink modes with low toroidal mode number n driven by the H-mode edge pressure gradient (and associated bootstrap current) which is experimentally evidenced by an observable shift of the poloidal mode number m away from field alignment (m = qn, with q being the safety factor) at the response maximum. A torque scan experiment demonstrates the importance of the perpendicular electron flow for shielding of the resonant magnetic perturbation, as expected from a two-fluid MHD picture. Two significant effects of MP occur in H-mode plasmas at low pedestal collisionality, $\nu _{\text{ped}}^{\ast}\leqslant 0.4$ : (a) a reduction of the global plasma density by up to $61 \% $ and (b) a reduction of the energy loss associated with edge localised modes (ELMs) by a factor of up to 9. A comprehensive database of ELM mitigation pulses at low ${{\nu}^{\ast}}$ in ASDEX Upgrade shows that the degree of ELM mitigation correlates with the reduction of pedestal pressure which in turn is limited and defined by the onset of ELMs, i. e. a modification of the ELM stability limit by the magnetic perturbation.
Evaluation of Dual Injection Methods for Multiple Zone Steamflooding (1989)

ABSTRACT For multiple oil zone reservoirs, it is advantageous to steam flood two zones at the same time to take advantage of overburden heating. To have adequate control over injection rates to each zone and provide injection streams with different steam qualities or water temperatures, two separate injection streams are required. With injection wells already in place, the most economical method is to use the same injection well to flood two zones simultaneously. Three dual injection methods were tried and tested at Texaco's Kern River Field in Bakersfield, California. Because dual injection results in an excessive amount of heat transfer between injection streams, it was imperative to predict steam quality or water temperature at the sandface for each injection stream. To adequately evaluate the merits of each method, a computer model was written to predict downhole temperatures, steam qualities, and pressures of each injection stream. Field tests of the three injection configurations provided measurements necessary for the validation of the model. This paper describes the computer model, the field test and the results from the three dual injection scenarios.
Cell polarity, cell adhesion, and spermatogenesis: role of cytoskeletons (2017)

In the rat testis, studies have shown that cell polarity, in particular spermatid polarity, to support spermatogenesis is conferred by the coordinated efforts of the Par-, Crumbs-, and Scribble-based polarity complexes in the seminiferous epithelium. Furthermore, planar cell polarity (PCP) is conferred by PCP proteins such as Van Gogh-like 2 (Vangl2) in the testis. On the other hand, cell junctions at the Sertoli cell-spermatid (steps 8-19) interface are exclusively supported by adhesion protein complexes (for example, α6β1-integrin-laminin-α3,β3,γ3 and nectin-3-afadin) at the actin-rich apical ectoplasmic specialization (ES) since the apical ES is the only anchoring device in step 8-19 spermatids. For cell junctions at the Sertoli cell-cell interface, they are supported by adhesion complexes at the actin-based basal ES (for example, N-cadherin-β-catenin and nectin-2-afadin), tight junction (occludin-ZO-1 and claudin 11-ZO-1), and gap junction (connexin 43-plakophilin-2) and also intermediate filament-based desmosome (for example, desmoglein-2-desmocollin-2). In short, the testis-specific actin-rich anchoring device known as ES is crucial to support spermatid and Sertoli cell adhesion. Accumulating evidence has shown that the Par-, Crumbs-, and Scribble-based polarity complexes and the PCP Vangl2 are working in concert with actin- or microtubule-based cytoskeletons (or both) and these polarity (or PCP) protein complexes exert their effects through changes in the organization of the cytoskeletal elements across the seminiferous epithelium of adult rat testes. As such, there is an intimate relationship between cell polarity, cell adhesion, and cytoskeletal function in the testis. Herein, we critically evaluate these recent findings based on studies on different animal models. We also suggest some crucial future studies to be performed.
Effect of Job Characteristics on Trait Anxiety and Task Performance of Private Security Workers (2011)

이 연구는 민간경비업 종사자의 직무특성이 특성불안 및 과업수행에 미치는 영향을 규명하고자 하였다. 이 연구는 2011년 서울 소재 경찰청에 등록된 민간경비 회사에 재직하고 있는 민간경비원을 대상으로 선정한 후 판단표집법(judgment sampling method)을 이용하여 총 300명을 표집하였으나, 최종 분석에 투입된 사례수는 266명이다. 이 연구에서는 설문지의 타당도 및 신뢰도를 검증하기 위하여 신뢰도분석의 알파값과 공변량구조분석의 최대우도법을 이용하였다. 이상과 같은 연구방법 및 절차를 통해 이 연구에서 도출한 결론은 다음과 같다. 첫째, 민간경비업 종사자의 과업중요성은 특성불안에 부적인 영향을 미친다. 둘째, 민간경비업 종사자의 피드백은 특성불안에 부적인 영향을 미친다. 셋째, 민간경비업 종사자의 직무자율성은 특성불안에 부적인 영향을 미친다. 넷째, 민간경비업 종사자의 피드백은 과업수행에 정적인 영향을 미친다. 다섯째, 민간경비업 종사자의 직무자율성은 과업수행에 정적인 영향을 미친다. 여섯째, 민간경비업 종사자의 기술다양성은 과업수행에 정적인 영향을 미친다. 일곱째, 민간경비업 종사자의 특성불안은 과업수행에 정적인 영향을 미친다. The study defines the job characteristics anxiety of the private security workers and the effect of the performance. While the study selected the private guards working at the private security companies registered at the National Police Agency in Seoul 2011 and sampled total 300 people by using the judgment sampling method, the final input case number is 266 people. The study used alpha value of the reliability analysis and the maximum-likelihood classification of the covariation structure analysis in order to verify the validity of the survey and the reliability. With the research method and the process the result of the study is as follows. First, the task importance of the private security workers affects the minus influence to the characteristic anxiety. Second, the feedback of the private security workers affects the minus influence to the characteristic anxiety. Third, the job autonomy of the private security workers affects the minus influence to the characteristic anxiety. Fourth, the feedback of the private security workers affects the plus influence to the task performance. Fifth, the job autonomy of the private security workers affects the plus influence to the task performance. Sixth, the skill variety of the private security workers affects the plus influence to the task performance. Seventh, the characteristic anxiety of the private security workers affects the plus influence to the task performance.
The TERAFLUX Project: Exploiting the DataFlow Paradigm in Next Generation Teradevices (2013)

Thanks to the improvements in semiconductor technologies, extreme-scale systems such as teradevices (i.e., composed by 1000 billion of transistors) will enable systems with 1000+ general purpose cores per chip, probably by 2020. Three major challenges have been identified: programmability, manageable architecture design, and reliability. TERAFLUX is a Future and Emerging Technology (FET) large-scale project funded by the European Union, which addresses such challenges at once by leveraging the dataflow principles. This paper describes the project and provides an overview of the research carried out by the TERAFLUX consortium.
Design of Simulation Test Platform for Charging Pile Control System (2020)

Abstract A fully functional, safe and stable electric vehicle charging pile is a necessary condition to ensure the widespread promotion of electric vehicles. At present, due to lack of testing methods, a large number of charging pile manufacturers have not performed a systematic test on their charging piles before leaving the factory. These defects make the real availability of charging piles put into operation in the market very low, and it is difficult to meet the charging needs of a large number of charged cars. To solve this problem, this research chooses to develop a simulation testing platform for charging pile control system based on semi-physical simulation technology.