gebhart/patrepeval huggingface dataset:
Structure of a membrane-attack complex/perforin (MACPF) family protein from the human gut symbiontBacteroides thetaiotaomicron (2010)

Membrane-attack complex/perforin (MACPF) proteins are transmembrane pore-forming proteins that are important in both human immunity and the virulence of pathogens. Bacterial MACPFs are found in diverse bacterial species, including most human gut-associated Bacteroides species. The crystal structure of a bacterial MACPF-domain-containing protein BT_3439 (Bth-MACPF) from B. thetaiotaomicron, a predominant member of the mammalian intestinal microbiota, has been determined. Bth-MACPF contains a membrane-attack complex/perforin (MACPF) domain and two novel C-terminal domains that resemble ribonuclease H and interleukin 8, respectively. The entire protein adopts a flat crescent shape, characteristic of other MACPF proteins, that may be important for oligomerization. This Bth-MACPF structure provides new features and insights not observed in two previous MACPF structures. Genomic context analysis infers that Bth-MACPF may be involved in a novel protein-transport or nutrient-uptake system, suggesting an important role for these MACPF proteins, which were likely to have been inherited from eukaryotes via horizontal gene transfer, in the adaptation of commensal bacteria to the host environment.
Secure Distributed Virtual Conferencing: Multicast or Bust (1999)

We describe a secure distributed virtual conferencing application (SDVC) that provides high quality streaming video and audio using IP multicast for efficient distribution, using strong authentication via cryptographic means and optionally providing fully encrypted communication without sacrificing quality of the medium or the user experience. We summarize our experiences with SDVC in a recent live demonstration and conclude with a discussion of future plans.
High contrast imaging testbed for the terrestrial planet finder coronagraph (2004)

One of the architectures under consideration for Terrestrial Planet Finder (TPF) is a visible coronagraph. To achieve TPF science goals, the coronagraph must have extreme levels of wavefront correction (less than 1 /spl Aring/ rms over controllable spatial frequencies) and stability to get the necessary suppression of diffracted starlight (10/sup -10/ contrast). The High Contrast Imaging Testbed is the TPF platform for laboratory validation of key coronagraph technologies, as well as demonstration of a flight-traceable approach to coronagraph implementation. Various wavefront sensing approaches are under investigation on the testbed, with wavefront control provided by a precision high actuator density deformable mirror. Diffracted light control is achieved through a combination of an occulting or apodizing mask and stop; many concepts exist for these components and are explored. Contrast measurements on the testbed establishes the technical feasibility of TPF requirements, while model and error budget validation are demonstrate implementation viability. This paper describes the current testbed design and preliminary experimental results.
Automatic generation of system test cases from use case specifications (2015)

In safety critical domains, system test cases are often derived from functional requirements in natural language (NL) and traceability between requirements and their corresponding test cases is usually mandatory. The definition of test cases is therefore time-consuming and error prone, especially so given the quickly rising complexity of embedded systems in many critical domains. Though considerable research has been devoted to automatic generation of system test cases from NL requirements, most of the proposed approaches re- quire significant manual intervention or additional, complex behavioral modelling. This significantly hinders their applicability in practice. In this paper, we propose Use Case Modelling for System Tests Generation (UMTG), an approach that automatically generates executable system test cases from use case spec- ifications and a domain model, the latter including a class diagram and constraints. Our rationale and motivation are that, in many environments, including that of our industry partner in the reported case study, both use case specifica- tions and domain modelling are common and accepted prac- tice, whereas behavioural modelling is considered a difficult and expensive exercise if it is to be complete and precise. In order to extract behavioral information from use cases and enable test automation, UMTG employs Natural Language Processing (NLP), a restricted form of use case specifica- tions, and constraint solving.
Mediated voice communication via mobile IP (2002)

Impromptu is a mobile audio device which uses wireless Internet Protocol (IP) to access novel computer-mediated voice communication channels. These channels show the richness of IP-based communication as compared to conventional mobile telephony, adding audio processing and storage in the network, and flexible, user-centered call control protocols. These channels may be synchronous, asynchronous, or event-triggered, or even change modes as a function of other user activity. The demands of these modes plus the need to navigate with an entirely non-visual user interface are met with a number of audio-oriented user interaction techniques.
Thiol–ene click chemistry for the design of diol porous monoliths with hydrophilic surface interaction ability: a capillary electrochromatography study (2016)

Thiol–ene click chemistry provides an efficient surface grafting strategy for designing diol monoliths meant for hydrophilic interaction capillary electrochromatography.
Faculty Opinions recommendation of The Fanconi anemia pathway promotes replication-dependent DNA interstrand cross-link repair. (2010)

Fanconi anemia is a human cancer predisposition syndrome caused by mutations in 13 Fanc genes. The disorder is characterized by genomic instability and cellular hypersensitivity to chemicals that generate DNA interstrand cross-links (ICLs). A central event in the activation of the Fanconi anemia pathway is the mono-ubiquitylation of the FANCI-FANCD2 complex, but how this complex confers ICL resistance remains enigmatic. Using a cell-free system, we showed that FANCI-FANCD2 is required for replication-coupled ICL repair in S phase. Removal of FANCD2 from extracts inhibits both nucleolytic incisions near the ICL and translesion DNA synthesis past the lesion. Reversal of these defects requires ubiquitylated FANCI-FANCD2. Our results show that multiple steps of the essential S-phase ICL repair mechanism fail when the Fanconi anemia pathway is compromised. PMID: 19965384 Funding information This work was supported by: NIGMS NIH HHS, United States Grant ID: R01 GM062267-09 NIGMS NIH HHS, United States Grant ID: R37 GM044664 Howard Hughes Medical Institute, United States NCI NIH HHS, United States Grant ID: T32 CA009216 NIGMS NIH HHS, United States Grant ID: R37 GM044664-23 NIGMS NIH HHS, United States Grant ID: R01 GM062267 NIGMS NIH HHS, United States Grant ID: GM62267 NCI NIH HHS, United States Grant ID: T32CA09216 More Less keyboard_arrow_down
Eight‐Membered and Larger Rings (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Magnetic structure of Co1-xMnx alloys (2001)

Co1-xMnx thin films epitaxially grown on GaAs(001) have been studied using reflection high-energy electron diffraction, x-ray diffraction, and the magneto-optical Kerr effect. The result shows that Co-rich films are body-centered cubic and ferromagnetic, and Mn-rich ones are face-centered cubic and antiferromagnetic. However, the film structure and magnetism at x similar to0.5 show strongly thickness-dependent behavior. The magnetic phases of ordered Co1-xMnx alloys (x = 0,1/4,1/2,3/4,1) in both bcc and fcc structures have also been investigated by a first-principles linearized augmented plane-wave calculation in the local-spin-density approximation. With the help of the calculated results, the correlation established in experiment between the structure and magnetism of Co1-xMnx alloys is better understood.
Kinetic study of the maltodextrine hydrolysis catalyzed by immobilized glucoamylase (1989)

International audience
Thermoelectric Power and Scattering of Carriers in Bi2?xSnxTe3 with Layered Structure (1997)

Thermoelectric power, electrical resistivity, and Hall effect of p-type Bi2—xSnxTe3 (0 ≤ x ≤ 0.030) single crystals have been measured in the temperature range 4.2 to 300 K. By doping Sn atoms into the host Bi2Te3 lattice, enhancements in the thermopower and resistivity are observed in the intermediate temperature range 30 to 150 K, and activation type behaviors are found in the resistivity versus temperature curves with an activation energy of the order of 10 meV which corresponds to the Sn-induced impurity band located above the second lower valence band. For our experimental results, we have also given a qualitative discussion about the scattering mechanism. Thermospannung, elektrischer Widerstand und Halleffekt von p-Bi2—xSnxTe3 (0 ≤ x ≤ 0,030) Kristallen werden bei Temperaturen von 4,2 bis 300 K gemessen. Die Dotierung mit Sn-Atomen in den Bi-Schichten von Bi2Te3 erhöht die Thermospannung und den Widerstand in einem mittleren Temperaturgebiet von 30 bis 150 K, und die Temperaturabhängigkeit des Hallkoeffizienten oder des Widerstands ergibt eine Aktivierungsenergie in der Größe von 10 meV, die dem Sn-induzierten Fremdband, das oberhalb des zweitniedrigsten Valenzbands liegt, entspricht. Für diese experimentellen Ergebnisse geben wir eine qualitative Diskussion über das Bandmodel und den Einfluß der Sn-Dotierung auf die elektronischen Eigenschaften.
Dynamics of variations of acute phase proteins in pigs fed Bioimmuno and/or immunised with Respisure One vaccine against mycoplasmal pneumonia (2011)

The purpose of the study was to determine the influence of the Bioimmuno administration in feed and/or immunisation with the ‘Respisure’ One vaccine against mycoplasmal pneumonia of swine on the development of non-specific immune response expressed by the level of acute phase proteins (APP). The study was performed on 28 piglets at the age of 4 weeks, divided into four equal groups. The biopreparations were administered according to the following pattern: group I - Bioimmuno (1 kg/50 kg of feedstuff) for 48 h and then vaccination with Respisure One (2 ml/animal i.m.), group II – Bioimmuno (1 kg/50 kg of feedstuff) for 48 h, group III - Respisure One (2 ml/animal i.m.), and group C (control) – PBS (2 ml/animal i.m.). Serum concentration of APP: Creactive protein, haptoglobin, and ceruloplasmin were determined on days 0, 3, 7, 14, and 21 after immunomodulation and/or immunisation. The statistically significantly lower (P<0.05) levels of all determined APP in the experimental groups in comparison with the control group were demonstrated. This proves the protective value of the biopreparations applied in specific patterns, through their beneficial influence on the decrease in consequences of the respiratory system infections in pigs.
Cerebrospinal Fluid Concentration of Brain-Derived Neurotrophic Factor and Cognitive Function in Non-Demented Subjects (2009)

Reduced CSF BDNF was associated with age-related cognitive decline, suggesting a potential mechanism that may contribute in part to cognitive decline in older individuals.
Supplementary Tables from TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem–Like Cells in a Redox-Sensitive Manner (2023)

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Plasma Immersion Ion Implantation: Innovative Technology for Surface Treatment of Stainless Steels* (2020)

Plasma immersion ion implantation technology was used to produce expanded austenite and to study the influence of treatment conditions and starting material on its formation and structure. Standard materials characterisation techniques, XRD, TEM, conversion electron Mössbauer spectroscopy, and Rutherford back-scatter spectrometry, analysis were used to determine the structure of the layers, thereby revealing the complex structure of expanded austenite. The properties of the expanded austenite layers (hardness, wear, and corrosion) were determined and correlated with the structure. Most work was concentrated on the austenitic stainless steel X6CrNiTil810 after treatments at 300 and 400°C. Based on these results, the possibilities of influencing properties by varying the process conditions are discussed.
Egr‐1 mediates the suppressive effect of IL‐1 on PPARg expression in human OA chondrocytes (2012)

Background Peroxisome proliferator‐activated receptor gamma (PPARg) is a ligand activated transcription factor. Several lines of evidence indicate that PPARg have protective effects in osteoarthritis (OA). We have previously shown that IL‐1 down‐regulated PPARg expression in OA chondrocytes. In the present study we will investigate the mechanisms underlying this effect of IL‐1. Methods Chondrocytes were stimulated with IL‐1, and the level of PPARg and Egr‐1 protein and mRNA were evaluated using WB and Real Time‐RT‐PCR, respectively. The PPARg promoter activity was analyzed in transient transfection experiments. Egr‐1 recruitment to the PPARg promoter was evaluated using ChIP assays. Small interfering RNA (siRNA) approaches were used to silence Egr‐1 expression. Results We demonstrated that the suppressive effect of IL‐1 on PPARg expression requires de novo protein synthesis. ChIP analyses revealed that IL‐1 induced Egr‐1 recruitment at the PPARg promoter. IL‐1 inhibited the activity of PPARg promoter and overexpression of Egr‐1 potentiated the inhibitory effect of IL‐1, suggesting that Egr‐1 may mediate the suppressive effect of IL‐1. Finally, Egr‐1 silencing with siRNA blocked IL‐1‐mediated down‐regulation of PPARg expression. Conclusion These results indicate that Egr‐1 contributes to IL‐1‐mediated down‐regulation of PPARg expression in OA chondrocytes.
Apical Spore Phagocytosis Is Not a Significant Route of Infection of Differentiated Enterocytes by Encephalitozoon intestinalis (2005)

Encephalitozoon intestinalis is a microsporidian species that infects the intestinal mucosal epithelium, primarily in immunodeficient individuals. The present study employed undifferentiated and differentiated human colonic carcinoma cell lines to determine if this parasite species infected polarized epithelial cells by spore phagocytosis or by impalement with the deployed spore polar tube. Apical surface spore attachment differed between cell lines such that SW480>HT-29>Caco-2>HCT-8, with attachment being greater to undifferentiated Caco-2 cells than differentiated cells and greater to partially differentiated HCT-8 cells than differentiated HCT-8 cells. Attachment was inhibited by chondroitin sulfate A, suggesting that it was mediated by host cell sulfated glycoaminoglycans. Infection rates 3 days postinfection paralleled spore attachment in the various cell lines. The undifferentiated cell line SW480 and undifferentiated Caco-2 and HCT-8 cells exhibited modest spore phagocytosis while the more differentiated cell line HT29 and differentiated Caco-2 and HCT-8 cells did not. All cell lines were impaled by the polar tubes of germinating spores. When normalized to the number of spores attached to the apical membrane, such impalement was greatest in the more differentiated Caco-2 and HCT-8 cells. The host cell apical surface influenced parasite spore germination, as in populations of large undifferentiated Caco-2 cells to which >3 spores had attached, the frequency distribution of the percentages of spores germinated per cell was bimodal, indicating that the surface of some cells favored germination, while others did not. This study suggests that phagocytosis is not a biologically significant mode of infection in differentiated intestinal epithelial cells.
Pressure intelligent control strategy of Waste heat recovery system of converter vapors (2013)

The converter gas evaporative cooling system is mainly used for absorbing heat in the high temperature exhaust gas which produced by the oxygen blowing reaction. Vaporization cooling steam pressure control system of converter is a nonlinear, time-varying, lagging behind, close coupling of multivariable control object. This article based on the analysis of converter operation characteristics of evaporation cooling system, of vaporization in a production run of pipe pressure variation and disturbance factors.For the dynamic characteristics of the controlled objects,we have improved the conventional PID control scheme.In Oxygen blowing process, we make intelligent control by using fuzzy-PID cascade control method and adjusting the Lance,that it can realize the optimization of the boiler steam pressure control.By design simulation, results show that the design has a good control not only ensures drum steam pressure in the context of security, enabling efficient conversion of waste heat.And the converter of 1800 flue gas through pipes and cool and dust removal also can be cooled to about 800. Therefore the converter haze evaporative cooling system has achieved to the converter haze temperature decrease effect and enhanced to the coal gas returns-ratio.
Identification and Solution Structure of a Highly Conserved C-terminal Domain within ORF1p Required for Retrotransposition of Long Interspersed Nuclear Element-1 (2007)

Long interspersed nuclear element-1 (LINE-1 or L1) retrotransposons comprise a large fraction of the human and mouse genomes. The mobility of these successful elements requires the protein encoded by open reading frame-1 (ORF1p), which binds single-stranded RNA with high affinity and functions as a nucleic acid chaperone. In this report, we have used limited proteolysis, filter binding, and NMR spectroscopy to characterize the global structure of ORF1p and the three-dimensional structure of a highly conserved RNA binding domain. ORF1p contains three structured regions, a coiled-coil domain, a middle domain of unknown function, and a C-terminal domain (CTD). We show that high affinity RNA binding by ORF1p requires the CTD and residues within an amino acid protease-sensitive segment that joins the CTD to the middle domain. Insights in the mechanism of RNA binding were obtained by determining the solution structure of the CTD, which is shown to adopt a novel fold consisting of a three-stranded beta sheet that is packed against three alpha-helices. An RNA binding surface on the CTD has been localized using chemical shift perturbation experiments and is proximal to residues previously shown to be essential for retrotransposition, RNA binding, and chaperone activity. A similar structure and mechanism of RNA binding is expected for all vertebrate long interspersed nuclear element-1 elements, since residues encoding the middle, protease-sensitive segment, and CTD are highly conserved.
Fluconazole and Risk for Adverse Pregnancy Outcomes (2019)

Fluconazole is most commonly prescribed for vaginal yeast infections, which can be resolved with a single 150-mg dose. Higher doses are indicated for
A study on an adaptive gait for a quadruped walking robot under external forces (2002)

In this presentation we propose an algorithm for the adaptive gait control by which a quadruped walking robot can walk against external disturbances. The proposed gait mechanism makes it possible for a quadruped walking robot to change its gait pattern in response to external disturbances from various external forces acting on the body of the walking robot. More specifically, we propose a new criterion for the stability margin of a walking robot under unknown external forces by using the effective mass center based on the zero moment point. And for the solution of the adaptive gait algorithm against external disturbances, a method of altitude control and reflexive direction control is suggested. Based on the algorithmic search method, an optimal stride of the quadruped walking robot that walks from any leg configuration to the standard configuration is also proposed. To verify the efficiency of the proposed approach, some simulation results are provided.
Titanium‐Mediated Domino Radical Cyclization/β Elimination of Phosphine Oxides (2004)

A phosphinoyl radical leaving group in the β position is a key feature of a new TiIII-mediated domino radical cyclization/β elimination reaction of vinylic phosphine oxides, which affords alkylidenepyrrolidines in good yields (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z460099_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Abnormal sodium transport in synaptosomes from brain of uremic rats. (1985)

The causes of central nervous system (CNS) dysfunction in uremia are not well known and are not completely reversed by dialysis. This problem was investigated in synaptosomes, which are membrane vesicles from synaptic junctions in the brain. We measured Na uptake under conditions of control, veratridine stimulation, and tetrodotoxin inhibition, in synaptosomes from normal and acutely uremic (blood urea nitrogen, 250 mg/dl) rats. In the control state, maximal Na uptake was 2.2 +/- 0.2 and 1.9 +/- 0.3 nmol/mg of protein in normal and uremic synaptosomes, respectively. With veratridine stimulation, Na uptake was increased by 1.9 and 3.6 nmol/mg of protein in normal vs. uremic rats (P less than 0.001). The increased veratridine-stimulated Na uptake observed in uremia could be due either to increased membrane permeability to Na or decrease in the Na-K ATPase pump activity. To investigate this, we studied the Na-K ATPase pump function by evaluating uptake of K (using rubidium as a tracer), uptake of Na during ATP stimulation, and inhibition of Rb and Na uptake by ouabain. In uremic rats both Rb uptake and ATP-stimulated Na uptake were significantly less than in normals (P less than 0.005). This suggests a defect in the Na-K ATPase pump. Membrane permeability for Na was then evaluated both by measuring initial Na uptake, and with addition of valinomycin. No change in Na uptake pattern was observed with valinomycin, and initial Na uptake was not significantly different in normal versus uremic synaptosomes. These data show that (a) in uremic rats veratridine-stimulated Na accumulation is significantly greater than normal; (b) the increased Na accumulation observed in uremia appears to be due to alterations in Na-K ATPase pump activity; and (c) the altered Na accumulation observed is probably not due to a uremic environment, but may be secondary to a physiologic alteration in synaptosomal function due to the uremic state. These abnormalities may affect neurotransmission and may be associated with the CNS alterations observed in uremia.
Towards External Varieties To Internal Varieties − Modular Perspective (2009)

Product customization is an essential requirement for manufacturing firms to achieve higher customers- satisfaction and fulfill business target. In order to achieve these objectives, firms need to handle both external varieties such as customer preference, government regulations, cultural considerations etc and internal varieties such as functional requirements of product, production efficiency, quality etc. Both of the varieties need to be accumulated and integrated together for the purpose of producing customized product. These varieties are presented and discussed in this paper along with the perspectives of modular product design and development process. Other development strategies such as modularity, component commonality, product family design and product platform are presented with a view to achieve product variety quickly and economically. A case example both for the concept of modular design and platform based product development process is also presented with the help of design structure matrix (DSM) tool. This paper is concluded with several managerial implications and future research direction.