gebhart/patrepeval huggingface dataset:
Synthesis of 1-Thio-Substituted Isoquinoline Derivatives by Tandem Cyclization of Isothiocyanates (2017)

A copper-catalyzed tandem arylation-cyclization process to access 1-(arylthio)isoquinolines from isothiocyanates and diaryliodonium salts is described. It is the first general method to construct the potentially useful 1-(arylthio)isoquinoline derivatives. Moreover, 1-(methylthio)isoquinoline derivatives were also achieved successfully with MeOTf instead of diaryliodonium salts under metal-free conditions. Mechanistic studies reveal that these two processes proceed in different routes. This method has been successfully applied to the synthesis of quinazolinone alkaloid rutaecarpine.
Characterization of RNA Invasion by 19F NMR Spectroscopy (2010)

(19)F NMR spectroscopy offers an efficient tool for monitoring RNA invasion. The invasion of 2'-O-methyl oligoribonucleotides into a (19)F-labeled HIV-1 TAR RNA model and the temperature-dependent behavior of the complexes obtained have been examined.
Surface plasmon enhanced photoluminescence of conjugated polymers (2007)

To produce surface plasmons, silver nanostructures were fabricated using a thermal evaporation method and the surface plasmon wavelength was tuned via an annealing. These nanostructures were located between the indium tin oxide (ITO) and poly(3,4-ethylenedioxy thiophene)/poly(styrenesulfonate) for the coupling of the surface plasmon resonance with organic fluorophores. To prevent the quenching of emission, spacer was placed between the light emitting polymers and the ITO substrate. As a result, the authors were able to observe an increase in photoluminescence of conjugated polymers using the silver nanostructures.
Remote Sensing Scene Classification Based on Improved GhostNet (2020)

Abstract Nowadays, the design of convolutional neural network (CNN) models is getting deeper and wider. When traditional CNN is used to process limited data of remote sensing images, it will lead to overfitting. We will use lightweight and efficient models to classify remote sensing images. In order to improve the classification accuracy and reduce the intermediate parameters, we improved GhostNet and proposed a smaller CNN named Improved GhostNet. Meanwhile, we use image enhancement methods to enlarge the datasets and dropout, it will reduce the amount of parameters. We experimented on three datasets, such as AID, UC Merced, NWPU-RESISC45. Then, we used MobileNetV3-Small and GhostNet to compare with our CNN model. The classification accuracy of improved GhostNet achieves more than 91%, and the accuracy on the AID is improved by 2.05% compared to the original GhostNet. These results demonstrate the effectiveness and efficiency of improved GhostNet.
Exploring New Scaffolds for the Dual Inhibition of HIV-1 RT Polymerase and Ribonuclease Associated Functions (2021)

Current therapeutic protocols for the treatment of HIV infection consist of the combination of diverse anti-retroviral drugs in order to reduce the selection of resistant mutants and to allow for the use of lower doses of each single agent to reduce toxicity. However, avoiding drugs interactions and patient compliance are issues not fully accomplished so far. Pursuing on our investigation on potential anti HIV multi-target agents we have designed and synthesized a small library of biphenylhydrazo 4-arylthiazoles derivatives and evaluated to investigate the ability of the new derivatives to simultaneously inhibit both associated functions of HIV reverse transcriptase. All compounds were active towards the two functions, although at different concentrations. The substitution pattern on the biphenyl moiety appears relevant to determine the activity. In particular, compound 2-{3-[(2-{4-[4-(hydroxynitroso)phenyl]-1,3-thiazol-2-yl} hydrazin-1-ylidene) methyl]-4-methoxyphenyl} benzamide bromide (<b>EMAC2063</b>) was the most potent towards RNaseH (IC<sub>50</sub> = 4.5 mM)- and RDDP (IC<sub>50</sub> = 8.0 mM) HIV RT-associated functions.
The Effect of Friction Stir Welding Process Parameters on Charpy V-Notch Impact Toughness in HSLA-65 (2012)

HSLA-65 steel (6.4 mm thick) was friction stir welded at various welding speeds and spindle speeds. Varying weld parameters provided a range of heat inputs. Impact toughness was evaluated as a function of the different weld parameters and corresponding weld heat inputs. Charpy V-Notch (CVN) tests were conducted in parent material and at both the weld nugget centerline and heat-affected zone (HAZ) locations. The upper shelf CVN impact energy of the weld nugget was above that of the base metal for all weld parameters. The upper shelf impact toughness in the HAZ was largely unaffected by changing weld parameters. The nil-ductility transition (NDT) temperature in the weld nugget increased with increasing heat input. The toughness, with respect to the ductile-to-brittle transition, was negatively affected by the increase in heat input. The NDT temperature in the HAZ did not correlate with heat input. The microstructures and microhardness data were examined. Aspects of variation in the impact energy results were identified as the inhomogeneity of the weld microstructure and the placement of the V-notch. Weld nugget microstructures were more inhomogeneous than base metal. Hardness results showed varying values of hardness from the weld crown to the root, transversely across the weld, and longitudinally along the length. Variation due primarily to the inhomogeneity of the weld microstructure is compounded by the location of the V-notch.
MAGE-D1 Regulates Expression of Depression-Like Behavior through Serotonin Transporter Ubiquitylation (2012)

The ubiquitin-proteasome system (UPS) controls the stability of most cellular proteins. The polymorphism of UPS-related genes is associated with major depression disorder, but less is known about the molecule that plays a role in depression by modulating the UPS. Melanoma antigen gene-D1 (MAGE-D1) interacts with RING E3 ubiquitin ligase and is implicated in protein degradation. MAGE-D1 may thus play an important role in the CNS via ubiquitylation. Here, we clarified a novel role of MAGE-D1 in emotional functions, namely its modulation of ubiquitylation to the serotonin transporter (SERT). The MAGE-D1 knock-out and knockdown by small interfering RNA (siRNA) in the prefrontal cortex showed depression-like behavior, such as a decrease in exploratory behavior in both the home cage and novel apparatus, a decrease in social interaction, increased immobility time during forced swimming and tail suspension, and a decrease in sucrose preference without any anxiety, or cognitive or motor dysfunction. Acute and chronic (28 d) administration of sertraline (10 mg/kg) and imipramine (20 mg/kg) reversed all or part of depression-like behavior in knock-out mice. In these mice, the serotonergic function in the prefrontal cortex and hippocampus was hypoactive, accompanied by hyperexpression of SERT attributable to a decrease in ubiquitylation. Furthermore, MAGE-D1 binds to SERT via the necdin homology domain. MAGE-D1 overexpression in cells resulted in a decrease in serotonin uptake activity and the protein level of SERT but an increase in ubiquitylated SERT. Together, the present findings suggest a novel role for MAGE-D1 in depressive behaviors: modulating SERT ubiquitylation.
Performance, reliability and life issues for components of the Planck sorption cooler (2002)

Continuous-duty hydrogen sorption cryocoolers are being developed for the European Space Agency (ESA) Planck mission. To achieve an acceptable level of performance and robust operation with these hydride refrigerators during flight, detailed investigations have been performed on the sorbent materials and on critical hardware components. The sorbent longevity has been verified for both the compressor alloy and the gas gap actuator alloy. Check valves that isolate the high- and low-pressure sides within the sorption compressor are potential single-point failures as internal leaks would short circuit hydrogen flow to the Joule-Thomson (J-T) expander. Check valves were operated using hydrogen gas for over 43,000 pressure cycles at various orientations and temperatures without leaks or other changes. The filters that will be used to protect check valves and J-T expander from particles were tested. The temperature gradients along the tubular heater elements for the compressor beds were evaluated to assess their impact on the dynamics of compressor element heating and hydrogen desorption. The durability and reliability of low-power heaters used for the gas gap actuators were determined by accelerated temperature cycling.
Mesenchymal stem cells isolated from fibrotic human lung tissue inhibit collagen expression by lung fibroblasts (2016)

<b>Introduction:</b> Mesenchymal stem cells have been identified in the human lung; however, their role in lung disease is not clear. In animal models of lung fibrosis, beneficial effects of exogenously administrated stem cells have been demonstrated. In this study we isolated and characterized mesenchymal stem cells from fibrotic human lung tissue and studied their properties <i>in vitro</i>. <b>Methods:</b> Mesenchymal stem cells were cultured from lung tissue obtained from patient with fibrotic lung diseases (n=10). The characterization of cells was performed by immunofluorescence stainings. The effect of mesenchymal stem cell-conditioned media on collagen mRNA expression by fibroblasts was studied by real time RT-PCR. Stem cell-conditioned medium was subjected to size-exclusion filtration and filtrates were used to determine their effect on collagen mRNA expression in fibroblasts. <b>Results:</b> Expression of CD44, CD90, CD105, Oct3/4, and NANOG characterized the cells as pluripotent cells of mesenchymal origin. Compared to control medium, mesenchymal stem cell-conditioned medium significantly inhibited the expression of collagen type I mRNA by fibroblasts by 40% (p&lt;0.0001). Filter experiments demonstrated that the stem cell-derived mediator(s) of the observed inhibitory effect on collagen expression must be &lt; 30 kDa. <b>Conclusions:</b> Our data demonstrate that conditioned medium obtained from lung mesenchymal stem cells exhibits anti-fibrotic properties via the inhibition of collagen expression by fibroblasts, suggesting that lung resident mesenchymal stem cells have fibro-protective properties and might therefore be a novel therapeutic approach for patients with idiopathic pulmonary fibrosis.
Calpain 3 Is a Rapid-Action, Unidirectional Proteolytic Switch Central to Muscle Remodeling (2010)

Calpain 3 (CAPN3) is a cysteine protease that when mutated causes Limb Girdle Muscular Dystrophy 2A. It is thereby the only described Calpain family member that genetically causes a disease. Due to its inherent instability little is known of its substrates or its mechanism of activity and pathogenicity. In this investigation we define a primary sequence motif underlying CAPN3 substrate cleavage. This motif can transform non-related proteins into substrates, and identifies >300 new putative CAPN3 targets. Bioinformatic analyses of these targets demonstrate a critical role in muscle cytoskeletal remodeling and identify novel CAPN3 functions. Among the new CAPN3 substrates are three E3 SUMO ligases of the Protein Inhibitor of Activated Stats (PIAS) family. CAPN3 can cleave PIAS proteins and negatively regulates PIAS3 sumoylase activity. Consequently, SUMO2 is deregulated in patient muscle tissue. Our study thus uncovers unexpected crosstalk between CAPN3 proteolysis and protein sumoylation, with strong implications for muscle remodeling.
Applied Materials And Methods For Surface Modification (2014)

Surface modification is very important in tailoring materials properties. Various methods and materials have been explored and developed to provide unique properties on different substrates for practical applications. One of the most effective methods is to use surface-grafting procedures to introduce functional coatings on various substrates. Here in this thesis, grafting techniques are applied to grow polymer brushes on substrates with selected surface morphologies, e.g. flat and spherical surfaces. By tailoring the functionalities of the polymers, desirable properties such as antifouling behavior and enhanced solubility can be specifically designed and achieved. These as-modified functional materials can be successfully applied to complex working environments, indicating the methods and materials can be used for additional promising surface modification processes. The first example is to functionalize reverse osmosis membranes with antifouling polymer brushes using a 'layer-by-layer' mediated method. This specific method not only protects the membrane surface from potential chemical damage, but also amplifies the reaction sites on the surface. Different antifouling polymer brushes were successfully grown on the flat membrane surfaces through both 'grafted to' and 'grafted from' approaches. The membrane exhibits improved antifouling properties against different kinds of bio-foulants with no change of water flux and salt rejection ratios. The second example is the synthesis of hybrid nanoparticles as potential tracers for enhanced oil recovery and carbon dioxide (CO2) sequestration. Fluorinated polymers were modified onto different nanoparticle spherical cores through a surface-initiated atomic transfer radical polymerization (SI-ATRP) reaction. Incorporation of fluorescent labels makes the nanoparticles easy to be detected. The as-prepared nanohybrids have diameters around 40 nanometers and the solubility of these nanoparticles in scCO2 has been significantly improved. Further application of the nanopraticles in diffusion tests shows that they can be applied as potential tracers in real geological oil fields. While surface-grafting techniques are one type of modification method, which in principal is to add coatigs on the substrates, another kind of modification methods is to remove coatings from a substrate using methods such as plasma or chemical etching. In this thesis, new etching processes are also investigated. Supercritical carbon dioxide is used as a processing medium to remove post-etch organic residue on silicon wafers and to etch titanium nitride (TiN) as well. scCO2 compatible quaternary ammonium salts were synthesized and applied with co-solvents to enhance the cleaning efficiency. Strong organic acids and peroxides, together with alcohols as stabilizers, were used to successfully etch TiN from silicon surfaces in both organic solvents and scCO2.
Role of Intraoperative Scrape Cytology in Diagnosis of Palpable Breast Lump (2019)

Background: Diseases of the breast constitute a significant proportion of surgical cases and frequently, the need arises to distinguish benign from malignant lesions prior to definitive treatment. So far, Frozen Section (FS) is the standard technique with high level of validity. However, intraoperative scrape cytology can be adopted when special facilities of FS are not available.&#x0D; Methods: This study designed with the aim to evaluate the value of Intraoperative Scrape Cytology (IOSC) in providing rapid and accurate diagnosis for breast lump and to compare its diagnostic yield with that of paraffin sections. This cross sectional descriptive study was carried out in the Department of Pathology, Chittagong Medical College in collaboration with Department of Surgery of this institution from October 2013 to September 2014. The study conducted on 123 patients with breast lumps who underwent operative treatment. Scrapings were taken from each specimen before formalin fixation and stained by rapid Papanicolaou staining. In each case their accuracy was compared to histopathological diagnosis. Statistical analysis done employing c2 test.&#x0D; Results: Out of 123 cases studied, 122 could be correctly differentiated into benign and malignant tumors with an accuracy rate of 99.19%.&#x0D; Conclusion: Intraoperative Scrape Cytology (IOSC) is a simple, accurate, rapid and cost-effective diagnostic tool, not requiring specific instruments in contrast to frozen section, can be used peroperatively for both diagnosis and management of breast lump.&#x0D; Chatt Maa Shi Hosp Med Coll J; Vol.17 (2); Jul 2018; Page 26-30
Nucleophile-assisted cleavage of silane cation radicals (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleophile-assisted cleavage of silane cation radicalsJoseph P. Dinnocenzo, S. Farid, J. L. Goodman, I. R. Gould, S. L. Mattes, and W. P. ToddCite this: J. Am. Chem. Soc. 1989, 111, 24, 8973–8975Publication Date (Print):November 1, 1989Publication History Published online1 May 2002Published inissue 1 November 1989https://pubs.acs.org/doi/10.1021/ja00206a061https://doi.org/10.1021/ja00206a061research-articleACS PublicationsRequest reuse permissionsArticle Views804Altmetric-Citations138LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Plasmodium falciparum protein associated with the invasion junction contains a conserved oxidoreductase domain (1995)

The merozoite cap protein-1 (MCP-1) of Plasmodium falciparum follows the distribution of the moving junction during invasion of erythrocytes. We have cloned the gene encoding this protein from a cDNA library using a monoclonal antibody. The protein lacks a signal sequence and has no predicted transmembrane domains; none of the antisera reacts with the surfaces of intact merozoites, indicating that the cap distribution is submembranous. MCP-1 is divided into three domains. The N-terminal domain includes a 52-amino-acid region that is highly conserved in a large family of bacterial and eukaryotic proteins. Based on the known functions of two proteins of this family and the pattern of amino acid conservation, it is predicted that this domain may possess oxido-reductase activity, since the active cysteine residue of this domain is invariant in all proteins of the family. The other two domains of MCP-1 are not found in any other members of this protein family and may reflect the specific function of MCP-1 in invasion. The middle domain is negatively charged and enriched in glutamate; the C-terminal domain is positively charged and enriched in lysine. By virtue of its positive charge, the C-terminal domain resembles domains in some cytoskeleton-associated proteins and may mediate the interaction of MCP-1 with cytoskeleton in Plasmodium.
Oxygen uptake kinetics as a determinant of very‐heavy intensity exercise tolerance (2008)

The tolerance profile for very‐heavy intensity exercise is well described by a hyperbola with two parameters: a curvature constant (W′) and a power asymptote (CP). During cycle ergometry, where work efficiency is similar between subjects, the tolerable duration (t lim ) is highly dependent on the rate at which oxidative ATP synthesis rises to meet the demand. We were therefore interested in the relationship between pulmonary oxygen uptake (VO 2 ) kinetics and the power‐duration hyperbola. We hypothesized that CP (the upper limit for steady‐state VO 2 ) would relate to the fundamental VO 2 time‐constant (τ), and W′ to the slow component magnitude (ΔVO 2sc ). Eight males performed repeats of constant work‐rate (WR) cycle ergometry to t lim : 1) over a range of WRs to estimate CP and W′ and 2) with WR normalized to induce intolerance in 6 min (WR 6 ; for τ and ΔVO 2sc ). VO 2 was measured breath‐by‐breath using a turbine and mass spectrometer. Tolerance at WR 6 averaged 365 ± 17 s. CP (range 181–289 W) was inversely correlated (R 2 = 0.37) with τ (21.3‐30.5 s); and W′ (12.8‐27.3 kJ) was strongly and directly correlated (R 2 = 0.97) with ΔVO 2sc (370–940 ml/min). Therefore, a fast τVO 2 was associated with a high CP thereby maximizing the WR for a given O 2 deficit, whilst a high rate of anaerobiosis ‐ reflected in W′ ‐ resulted in a large ΔVO 2sc . Together, this suggests that VO 2 kinetics are a major determinant of tolerance during very‐heavy intensity exercise.
High-Reflectivity Two-Axis Vertical Comb Drive Microscanners for Confocal Imaging Applications (2006)

We present 500times700 mum metal-coated scanning micromirrors fabricated from bonded SOI-Si wafers with ~90% reflectivity at 633 nm. Confocal images with 1 mum resolution were generated using single axis actuation of plusmn2.5deg at 1.87 KHz
Incorporation of CNF with Different Charge Property into PVP Hydrogel and Its Characteristics (2021)

Cellulose nanofibril (CNF)-added polyvinylpyrrolidone (PVP) hydrogels were prepared using different types of CNFs and their properties were investigated. CNFs with different morphology and surface charge properties were prepared through quaternization and carboxymethylation pretreatments. The quaternized CNF exhibited the narrow and uniform width, and higher viscoelastic property compared to untreated and carboxymethylated CNF. When CNF was incorporated to PVP hydrogel, gel contents of all hydrogels were similar, irrespective of CNF addition quantity or CNF type. However, the absorptivity of the hydrogels in a swelling medium increased by adding CNF. In particular, the quaternized CNF-added PVP hydrogel exhibited the highest swelling ability. Unlike that of hydrogels with untreated and carboxymethylated CNFs, the storage modulus of PVP hydrogels after swelling significantly increased with an increase in the content of the quaternized CNF. These indicate that a PVP hydrogel with a high absorptivity and storage modulus can be prepared by incorporating the proper type of CNF.
Alarm Fatigue: A Risk Assessment (2013)

Alarm fatigue is the phenomenon which occurs when nurses become overwhelmed by the high number of alarms in the clinical environment. This is a significant patient safety issue as delayed or inappropriate responses can and have resulted in patient harm. The purpose of the observational study was to conduct a risk assessment of alarm fatigue at an acute care teaching hospital in Providence, RI. Observations of telemetry alarms and response were conducted, utilizing a standardized tool on two medical surgical units over a six week time period. Participants were 36 nurses working on the two units at time of observations. Alarms were quantified to determine the percentage of false, technical, valid, and nuisance alarms. Alarm frequency was calculated and average response time to critical and leads off alarms were determined. Nurses were found to be at risk for and experiencing alarm fatigue based on high alarm frequency, increased number of false and nuisance alarms, and a delayed response to leads off conditions. The findings in this study are consistent with what is occurring in healthcare organizations nationally, as evidenced by a recent Joint Commission Sentinel Event Alert about medical device alarm safety that cited alarm fatigue as a major contributing factor. Recommendations and implications are presented and discussed.