gebhart/patrepeval huggingface dataset:
Design and Implementation of a Network Management System for Service Oriented Network (2012)

Today, with the advent of cloud computing and the growth of mobile services, a new Internet paradigm is required that is capable of providing an on-demand service by discovering and aggregating service resources for users. A Service Oriented Network (SON)[1] is focused on the services provided over a network rather than the technologies or their related components. These technologies or components are hidden from the end users and third parties so their only concern is how to get and use the services on any device, anywhere, and at anytime of the day. From a network management point of view, the task of network management for a SON will become increasingly complex since a number of services will be distributed and the required components for the services can be dynamically integrated to fulfill the service requests in a real-time manner. Therefore, an efficient network management system with a highly visualized Graphical User Interface (GUI) and an optimal network architecture must be deployed to enhance the SON. We therefore design and implement a GUI-based network management system to manage the SON.
Pharmacological characterization of small‐conductance Ca2+‐activated K+ channels stably expressed in HEK 293 cells (2000)

Three genes encode the small-conductance Ca(2+)-activated K(+) channels (SK channels). We have stably expressed hSK1 and rSK2 in HEK 293 cells and addressed the pharmacology of these subtypes using whole-cell patch clamp recordings. The bee venom peptide apamin blocked hSK1 as well as rSK2 with IC(50) values of 3.3 nM and 83 pM, respectively. The pharmacological separation between the subtypes was even more prominent when applying the scorpion peptide blocker scyllatoxin, which blocked hSK1 with an IC(50) value of 80 nM and rSK2 at 287 pM. The potent small molecule blockers showed little differentiation between the channel subtypes. The bis-quinolinium cyclophane UCL 1684 blocked hSK1 with an IC(50) value of 762 pM and rSK2 at 364 pM. The antiseptic compound dequalinium chloride blocked hSK1 and rSK2 with IC(50) values of 444 nM and 162 nM, respectively. The nicotinic acetylcholine receptor antagonist d-tubocurarine was found to block hSK1 and rSK2 with IC(50) values of 27 microM and 17 microM when measured at +80 mV. The inhibition by d-tubocurarine was voltage-dependent with increasing affinities at more hyperpolarized potentials. The GABA(A) receptor antagonist bicuculline methiodide also blocked hSK1 and rSK2 in a voltage-dependent manner with IC(50) values of 15 and 25 microM when measured at +80 mV. In conclusion, the pharmacological separation between SK channel subtypes expressed in mammalian cells is too small to support the notion that the apamin-insensitive afterhyperpolarization of neurones is mediated by hSK1.
Collagen turnover is associated with cardiovascular autonomic and peripheral neuropathy in type 1 diabetes: novel pathophysiological mechanism? (2023)

Results show previously undescribed associations between markers of collagen turnover and risk of CAN and to a lesser degree DSPN in T1D.
Conduite à tenir devant un œdème papillaire de l’enfant (2015)

La decouverte d’un œdeme papillaire chez l’enfant constitue une urgence neuro-ophtalmologique, a double titre : un œdeme papillaire peut conduire a une atrophie optique ; de plus, sa cause peut mettre en jeu le pronostic vital de l’enfant. L’histoire clinique et les signes fonctionnels associes permettent souvent une orientation diagnostique rapide.Tout œdeme papillaire ne resulte pas d’une hypertension intracrânienne : le contexte, un examen ophtalmologique specialise avec etude soigneuse des milieux oculaires a la lampe a fente, au besoin des examens complementaires orientes, permettent de poser (ou de suspecter) les diagnostics differentiels classiques : drusen enfouies de la papille optique ; pseudo-œdemes papillaires constitutionnels (generalement asymptomatiques chez l’enfant) ; infiltrations du nerf optique dans un contexte tumoral ou metabolique ; papillite dans le cadre d’une uveite posterieure ; nevrite optique inflammatoire ou infectieuse. Le diagnostic d’œdeme papillaire de stase secondaire a une hypertension intracrânienne est clinique – l’imagerie cerebrale peut etre qualifiee de normale – et la conduite a tenir chez l’enfant bien codifiee.
Determination of an Optimal Threshold Value for Muscle Activity Detection in EMG Analysis. (2010)

It is commonly agreed that one needs to use a threshold value in the detection of muscle activity timing in electromyographic (EMG) signal analysis. However, the algorithm for threshold determination lacks an agreement between the investigators. In this study we aimed to determine a proper threshold value in an incremental cycling exercise for accurate EMG signal analysis. Nine healthy recreationally active male subjects cycled until exhaustion. EMG recordings were performed on four low extremity muscle groups; gastrocnemius lateralis (GL), gastrocnemius medialis (GM), soleus (SOL) and vastus medialis (VM). We have analyzed our data using three different threshold levels: 25%, 35% and 45% of the mean RMS EMG value. We compared the appropriateness of these threshold values using two criteria: (1) significant correlation between the actual and estimated number of bursts and (2) proximity of the regression line of the actual and estimated number of bursts to the line of identity. It had been possible to find a significant correlation between the actual and estimated number of bursts with the 25, 35 and 45% threshold values for the GL muscle. Correlation analyses for the VM muscle had shown that the number of bursts estimated with the 35% threshold value was found to be significantly correlated with the actual number of bursts. For the GM muscle, it had been possible to predict the burst number by using either the 35% or 45% threshold value and for the SOL muscle the 25% threshold value was found as the best predictor for actual number of burst estimation. Detailed analyses of the actual and estimated number of bursts had shown that success of threshold estimation may differ among muscle groups. Evaluation of our data had clearly shown that it is important to select proper threshold values for correct EMG signal analyses. Using a single threshold value for different exercise intensities and different muscle groups may cause misleading results. Key pointsα priori accepted threshold value may cause erroneous results in EMG analysis.Using a single threshold value for different exercise intensities and different muscle groups may cause misleading results.The investigators may need to use different threshold selection strategies for different workloads.The investigators have to justify the choice of threshold selection with valid arguments before detailed EMG signal analyses.
Studies on Mutarotases (1969)

Abstract Mutarotase (aldose 1-epimerase) was isolated from bovine kidney cortex in a 10-stage procedure with conventional techniques. The final product which appears homogeneous in gel diffusion patterns and in sedimentation patterns on sucrose density gradients has a molecular weight of approximately 40,000. Turnover numbers and Km values for the five substrate sugars were d-glucose (1.1 x 106 min-1, 25.2 mm), d-galactose (1.3 x 106 min-1, 6.5 mm), d-xylose (1.3 x 106 min-1, 13.2 mm), l-arabinose (2.0 x 106 min-1, 8.3 mm), and d-fucose (0.35 x 106 min-1, 2.0 mm). Dialysis against water inactivated the enzyme while 10-5 m NaCl restored 50% activity. The preparation is completely stable at 4° but loses activity when stored frozen. It is reversibly inactivated by traces of organic solvents. The enzyme is sulfhydryl-dependent and was completely inactivated by mercuric ions or p-chloromercuribenzoate. The sulfhydryl content measured by titration with p-chloromercuribenzoate was 8 moles of cysteine per mole of enzyme and preliminary amino acid analyses indicated the presence of approximately 18 moles of histidine. Structural requirements for substrates are a pyranose ring with free hydroxyl groups on C-2 and C-3 in the equatorial-equatorial (trans) configuration. Many sugars, including enantiomorphs of the substrates, are good competitive inhibitors. The steric requirements indicate that a three-point attachment to the enzyme is required for substrate activity but that a two-point attachment at C-2 or C-3 or the ring oxygen (or both) is sufficient for inhibition.
Particle-size dependence of magnetization relaxation inMn12crystals (2009)

We show that reducing the crystal size of $[{\text{Mn}}_{12}{\text{O}}_{12}{({\text{O}}_{2}{\text{C}}_{6}{\text{H}}_{5})}_{16}{({\text{H}}_{2}\text{O})}_{4}]$ single-molecule magnets from 11.5 to $0.4\text{ }\ensuremath{\mu}\text{m}$ strongly affects the molecular magnetic anisotropy and magnetic-relaxation rates. The effective activation energy for the spin reversal of the standard clusters decreases by 13% with decreasing size, whereas it remains approximately constant for the ``fast relaxing'' species. The pre-exponential factor ${\ensuremath{\tau}}_{0}$ increases with decreasing crystal size for both. The observed decrease in the effective energy barrier for the slow relaxing species seems to be associated with the existence of a distribution of second-order transverse anisotropy terms, centered on $E=0$, which broadens as the crystal size decreases. By contrast, the expected changes in the axial anisotropy parameter $D$ with decreasing crystal size are too small to account for the change in $U$. The different effects that the reduction in crystal size has on the fast and slow relaxing species are discussed.
Review on stationary phases and coating methods of MEMs gas chromatography columns (2020)

Abstract Gas chromatography (GC) is an important and widely used technique for separation and analysis in the field of analytical chemistry. Micro gas chromatography has been developed in response to the requirement for on-line analysis and on-site analysis. At the core of micro gas chromatography, microelectromechanical systems (MEMs) have the advantages of small size and low power consumption. This article introduces the stationary phases of micro columns in recent years, including polymer, carbon materials, silica, gold nanoparticles, inorganic adsorbents and ionic liquids. Preparation techniques ranging from classical coating to unusual sputtering of stationary phases are reviewed. The advantages and disadvantages of different preparation methods are analyzed. The paper introduces the separation characteristics and application progress of MEMs columns and discusses possible developments.
A spatially heterogeneous network-based metapopulation software model applied to the simulation of a pulmonary tuberculosis infection (2018)

Tuberculosis (TB) is an ancient disease that, although curable, still accounts for over 1 million deaths worldwide. Shortening treatment time is an important area of research but is hampered by the lack of models that mimic the full range of human pathology. TB shows distinct localisations during different stages of infection, the reasons for which are poorly understood. Greater understanding of how heterogeneity within the human lung influences disease progression may hold the key to improving treatment efficiency and reducing treatment times. In this work, we present a novel <i>in silico</i> software model which uses a networked metapopulation incorporating both spatial heterogeneity and dissemination possibilities to simulate a TB infection over the whole lung and associated lymphatics. The entire population of bacteria and immune cells is split into a network of patches: members interact within patches and are able to move between them. Patches and edges of the lung network include their own environmental attributes which influence the dynamics of interactions between the members of the subpopulations of the patches and the translocation of members along edges. In this work, we detail the initial findings of a whole-organ model that incorporates distinct spatial heterogeneity features which are not present in standard differential equation approaches to tuberculosis modelling. We show that the inclusion of heterogeneity within the lung landscape when modelling TB disease progression has significant outcomes on the bacterial load present: a greater differential of oxygen, perfusion and ventilation between the apices and the basal regions of the lungs creates micro-environments at the apex that are more preferential for bacteria, due to increased oxygen availability and reduced immune activity, leading to a greater overall bacterial load present once latency is established. These findings suggest that further whole-organ modelling incorporating more sophisticated heterogeneities within the environment and complex lung topologies will provide more insight into the environments in which TB bacteria persist and thus help develop new treatments which are factored towards these environmental conditions.
Recombinant Vaccinia Viruses as New Live Vaccines (1984)

Recombinant vaccinia viruses can be constructed that express foreign antigens. These viruses retain their infectivity and synthesise the foreign gene product in tissue culture and in vaccinated animals. Following vaccination, specific antibody and cell-mediated immune responses are generated against the foreign protein and in several cases these have protected the animal against subsequent challenge with the corresponding pathogen. The potential use of recombinant vaccinia viruses as medical or veterinary vaccines is discussed.
Comparing Process Efficiency in Reducing Steryl Glucosides in Biodiesel (2009)

Abstract A significant obstacle to the commercial acceptance of biodiesel is the potential for filter plugging due to precipitates in the fuel. The majority of these precipitates can be attributed to either steryl glucosides (SGs) or monoacylglycerols in biodiesel. A GC–FID method to quantify minor components content in biodiesel is presented. The effectiveness of room temperature and cold soak filtration, adsorbent treatment, centrifuge, and vacuum distillation processes for SG removal was studied. The vacuum distillation process is the most effective method of removing the SG from biodiesel.
Name-It: association of face and name in video (2002)

This paper proposes a novel approach to extract meaningful content information from video by collaborative integration of image understanding and natural language processing. As an actual example, we developed a system that associates faces and names in videos, called Name-It, which is given news videos as a knowledge source, then automatically extracts face and name association as content information. The system can infer the name of a given unknown face image, or guess faces which are likely to have the name given to the system. This paper explains the method with several successful matching results which reveal effectiveness in integrating heterogeneous techniques as well as the importance of real content information extraction from video, especially face-name association.
Cell-free translocation of recombinant p47-phox, a component of the neutrophil NADPH oxidase: effects of guanosine 5'-O-(3-thiotriphosphate), diacylglycerol, and an anionic amphiphile (1992)

We reported previously that diacylglycerol (diC8) and GTP gamma S synergize with an anionic amphiphile such as sodium dodecyl sulfate (SDS) to produce high rates of superoxide generation in a cell-free system consisting of neutrophil plasma membrane plus cytosol [Burnham, D. N., Uhlinger, D. J., & Lambeth, J. D. (1990) J. Biol. Chem. 265, 17550-17559]. Here we investigate the effects of these activating factors on the plasma membrane association in an in vitro translated radiolabeled recombinant p47-phox protein. Apparent translocation, assayed by cosedimentation with plasma membranes, required the presence of excess cytosol and an anionic amphiphile, was enhanced by both GTP gamma S and diC8, and was inhibited by high salt, correlating qualitatively with activation; up to 70% cosedimentation was observed with the combination of activators (compared with less than 20% in their absence). Similar results were obtained using heat-inactivated cytosol, wherein another oxidase component, p67-phox, has been inactivated. Unexpectedly, from 50 to 80% of the apparent translocation occurred in the absence of membranes, indicating that protein aggregation accounted for a significant part of the observed translocation. Nevertheless, the percent translocation was increased in all cases by the presence of membranes, indicating some degree of protein-membrane interaction. While a control in vitro translated protein failed to translocate, cosedimentation of p47-phox occurred equally well when red blood cell or neutrophil plasma membranes lacking cytochrome b558 were used. Also, the peptide RGVHFIF, which is contained within the C-terminus of the large subunit of cytochrome b558, failed to inhibit translocation/aggregation of p47-phox, despite its ability to inhibit cell-free activation of the oxidase. The data are consistent with the following: (a) SDS, diC8, and GTP gamma S all act on cytosolic components to alter protein-protein and/or protein-membrane associations, and these changes are necessary (but not sufficient) for activation; (b) these altered associations are likely to function by increasing the local concentration of p47-phox and other components at the plasma membrane; (c) a high background of nonspecific associations in the cell-free activation system is likely to obscure any specific, functionally relevant associations (e.g., with cytochrome b558); and (d) the mechanism of translocation in the cell-free system differs from that seen in intact neutrophils.
Robust servo-system based on two-degree-of-freedom control with sliding mode (2002)

A robust-servo-system, based on a combination of disturbance observer and sliding mode control have been proposed. In this paper, disturbance observer is designed on the parametrization of two-degree-of-freedom control system. Through the simulations and experiments, it was proved that the introduction of nonlinear control (sliding mode) improves the disturbance suppression characteristics of a linear system with a disturbance observer.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Discriminative Stimulus Properties of Opioid Ligands: Progress and Future Directions (2016)

Opioid receptors (MOP-r, KOP-r, DOP-r, as well as NOP-r) and their endogenous neuropeptide agonist systems are involved in diverse neurobiological and behavioral functions, in health and disease. These functions include pain and analgesia, addictions, and psychiatric diseases (e.g., depression-, anxiety-like, and stress-related disorders). Drug discrimination assays have been used to characterize the behavioral pharmacology of ligands with affinity at MOP-r, KOP-r, or DOP-r (and to a lesser extent NOP-r). Therefore, drug discrimination studies with opioid ligands have an important continuing role in translational investigations of diseases that are affected by these neurobiological targets and their pharmacotherapy.
Chlorpromazine binding to the PAS domains uncovers the effect of ligand modulation on EAG channel activity (2020)

Ether-a-go-go (EAG) potassium selective channels are major regulators of neuronal excitability and cancer progression. EAG channels contain a Per-Arnt-Sim (PAS) domain in their intracellular N-terminal region. The PAS domain is structurally similar to the PAS domains in non-ion channel proteins, where these domains frequently function as ligand-binding domains. Despite the structural similarity, it is not known whether the PAS domain can regulate EAG channel function via ligand binding. Here, using surface plasmon resonance, tryptophan fluorescence, and analysis of EAG currents recorded in <i>Xenopus laevis</i> oocytes, we show that a small molecule chlorpromazine (CH), widely used as an antipsychotic medication, binds to the isolated PAS domain of EAG channels and inhibits currents from these channels. Mutant EAG channels that lack the PAS domain show significantly lower inhibition by CH, suggesting that CH affects currents from EAG channels directly through the binding to the PAS domain. Our study lends support to the hypothesis that there are previously unaccounted steps in EAG channel gating that could be activated by ligand binding to the PAS domain. This has broad implications for understanding gating mechanisms of EAG and related ERG and ELK K<sup>+</sup> channels and places the PAS domain as a new target for drug discovery in EAG and related channels. Up-regulation of EAG channel activity is linked to cancer and neurological disorders. Our study raises the possibility of repurposing the antipsychotic drug chlorpromazine for treatment of neurological disorders and cancer.
Optimization of Hydro-Fracturing Fluid for Enhanced Well Performance in Deep Volcanic Gas Reservoir (2016)

Abstract The Raageshwari Deep Gas (RDG) field in Barmer Basin was discovered in 2003 by Cairn India. The RDG field has excellent gas quality with low CO2 and no H2S. The average well depth is 3300m with bottomhole pressure of 5500 psi and bottomhole temperature of 123degC – 135 degC. Typical gas production rate is up to 12 MMSCFD with CGR of 50-60 bbl/MMSCF during initial well testing. The field is developed with 3.5" monobore vertical wells suitable for multistage hydraulic fracturing. Hydraulic fracturing treatment is required as thin laminated volcanic comprise most of the reservoir section having permeability of ~ 0.1 mD. Till date, Cairn India the operator has undertaken four hydraulic fracturing campaigns in Raageshwari gas field with over 100 successful fracs pumped in 25 wells. One of the critical components that lead to a successful hydro fracturing job is fracturing fluid. The properties of the fracturing fluid impact the fracture geometry, the proppant pack conductivity, and ultimately the productivity of the well. Extensive lab tests were done to determine the optimum fracturing fluid recipe to achieve multiple objectives such as minimum viscosity requirement, clay stabilization, effective breaking of fluid, increased fluid recovery, etc. Concentration of various additives were determined in conjunction through extensive laboratory testing. Further flowback samples were collected and tested to evaluate the correct recipe which was most suitable for Raageshwari gas wells hydraulic fracturing treatments. The current frac fluid design is the result of continuous improvement/learnings from the successive frac campaigns in Raageshwari gas field and has resulted in improved frac fluid recovery and overall well performance. This paper summarizes the basis of fluid design and various imporovements made in the frac fluid that was used in Raageshwari field. Key results and findings are discussed from over 65 lab tests performed for fluid optimization.