gebhart/patrepeval huggingface dataset:
Proton-Induced Permeability and Fusion of Large Unilamellar Vesicles by Covalently Conjugated Poly(2-Ethylacrylic Acid) (1999)

Proton sensitive large unilamellar vesicles (LUV) were constructed by immobilization of the pH sensitive synthetic polymer poly(2-ethylacrylic acid) onto the outer monolayer. Thiolated poly(2-ethylacrylic acid) (PEAA-SH) was covalently conjugated to the surface of LUVs composed of egg phosphatidylcholine (EPC) and cholesterol (Choi) through the thiol-reactive maleimide lipid MPB-DSPE (N-(4-(p-maleimidophenyl)butyryl)-1,2-distearoyl-sn-glyc-ero-3-phosphoethanolamine). The resulting PEAA- LUVs were shown to be stable at neutral pH (pH 7.0 to 8.0). Under acidic conditions, however, proton-ation of PEAA resulted in interaction with both the membrane it was linked to and the membrane of target vesicles, causing membrane destabilization and release of vesicle contents. Moreover, conjugated PEAA is shown to mediate fusion with target membranes in a pH dependent manner. PEAA-mediated permeabilization and vesicle-vesicle fusion occurred only when the polymer was covalently linked to the LUV surface. Proton dependent fusion of PEAA-LUVs was also observed with erythrocyte ghosts. This pH-dependent release of vesicle contents and fusion of PEAA-LUVs occurred below pH 6.8, which is well within the pH range expected to be encountered inside the endosomes in the endocytic pathway, indicating the potential of PEAA-LUVs as a drug carrier system for intracellular drug delivery.
Cloning of a Tobacco Apoplasmic Invertase Inhibitor1 (1998)

Higher plants express several isoforms of vacuolar and cell wall invertases (CWI), some of which are inactivated by inhibitory proteins at certain stages of plant development. We have purified an apoplasmic inhibitor (INH) of tobacco (Nicotiana tabacum) CWI to homogeneity. Based on sequences from tryptic fragments, we have isolated a full-length INH-encoding cDNA clone (Nt-inh1) via a reverse transcriptase-polymerase chain reaction. Southern-blot analysis revealed that INH is encoded by a single- or low-copy gene. Comparison with expressed sequence tag clones from Arabidopsis thaliana and Citrus unshiu indicated the presence of Nt-inh1-related proteins in other plants. The recombinant Nt-inh1-encoded protein inhibits CWI from tobacco and Chenopodium rubrum suspension-cultured cells and vacuolar invertase from tomato (Lycopersicon esculentum) fruit, whereas yeast invertase is not affected. However, only in the homologous system is the inhibition modulated by the concentration of Suc as previously shown for INH isolated from tobacco cells. Highly specific binding of INH to CWI could be shown by affinity chromatography of a total cell wall protein fraction on immobilized recombinant Nt-inh1 protein. RNA-blot analysis of relative transcript ratios for Nt-inh1 and CWI in different parts of adult tobacco plants revealed that the expression of both proteins is not always coordinate.
Distinction Between Homozygous and Heterozygous Subjects with Hereditary Haemochromatosis Using Iron Status Markers and Receiver Operating Characteristic (ROC) Analysis (1995)

The purpose of the present study was: 1) to evaluate which of the four iron status markers (serum iron, serum transferrin, serum transferrin saturation, serum ferritin) displayed the highest discriminatory potential in the distinction between homozygous patients with hereditary haemochromatosis and heterozygous relatives, 2) to suggest optimum cut-off values for these iron status markers, and 3) to demonstrate how these cut-off values change if the expected utility from a correct diagnosis is incorporated into the analysis. The patients and relatives were found by a nation-wide epidemiological survey. The study population consisted of 162 patients with clinically overt hereditary haemochromatosis and 84 asymptomatic heterozygous relatives. The statistical evaluation was performed using receiver operating characteristic (ROC) curve analysis. The diagnostic power of the iron markers is expressed as the area under the ROC curve. The optimum cut-off value is at the point where the slope of the ROC curve is equal to one. Changes in the optimum cut-off value at varying expected utility from a correct classification was estimated by changing the scaling of the ROC diagram. Serum iron and serum transferrin had the smallest area under the ROC curve, and were both unsuitable as discriminators. Near complete discrimination was obtained with serum transferrin saturation and serum ferritin concentrations, displaying the largest area under the ROC curve (0.991 and 0.998). The optimum threshold value for transferrin saturation was 61%, and for serum ferritin concentration 800 micrograms/l. The transferrin saturation level reflects the presence of the haemochromatosis allele, whereas the serum ferritin concentration indicates the degree of iron overload.(ABSTRACT TRUNCATED AT 250 WORDS)
Analysis of the Effects of 3DP Parameters on Part Feature Dimensional Accuracy (2007)

3D printing (3DP) is a widely investigated scaffold manufacturing process for Tissue Engineering (TE). Useful scaffold geometries should have high porosity (60-80%) with small (100-500 μm) interconnected pores. Therefore dimensional accuracy on the micron level is one of the crucial parameters of the bone scaffolds. Previously it was shown that the behavior of scaffold geometries can be well simulated with Finite Element Modeling (FEM) however the prediction of actual strength and stiffness values are dependent on dimensional accuracy. This accuracy is in turn dependent on several parameters including particle size and shape, powderbinder interaction, and machine setup. In this work different scaffold strut sizes (0.3 - 0.5 mm) have been fabricated using two different plaster powders (zp102 and zp130) with variations in shell saturation levels, part print position, and part print orientation. The parameters for each powder were analyzed using a full 35 factorial experimental design. It was found that the part size and orientation had a significant effect on the dimensional accuracy while the influence of the shell saturation and position was relatively small. The results allow for better dimensional specification for scaffold geometry fabrication by defining the process parameters in 3DP that may be used further in scaffold accuracy optimization.
A Horizontal Well in Gas Storage: A Case Study (1993)

ABSTRACT Enhancement of deliverability has always been a top priority for storage field operators. Historically, all available enhancement techniques have been tried, with varying and occasionally negative results. The recent and rapid development of horizontal drilling technologies has given storage operators another method for consideration. There are several geological and reservoir engineering aspects in evaluating a potential horizontal storage well project. If favorable conditions exist, a multidisciplinary approach is needed for the design and implementation of a successful project. This paper discusses the methodology and results of a successful horizontal storage well project conducted jointly by Oklahoma Natural Gas Company ("ONG") and the Gas Research Institute ("GRI").
Towards A Graphical And Real-time NetworkSimulation Toolset (1970)

Using simulation to model complex engineering systems can provide cost-savings for testing, while being able to stretch boundaries not normally feasible on actual system tests. The authors have been involved in researching and applying a toolset to provide such testing, in terms of behaviour analysis, for SDH Multiplexer telecommunications systems. This actual simulation has been implemented on transputers to provide scaleable and flexible topology emulation and a KBS tool has been prototyped to perform intelligent mapping between the desired SDH topology and transputer simulation.
Effect of Passive Immunotherapy on Murine Gut-Derived Sepsis Caused by Pseudomonas Aeruginosa (1999)

The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampicillin (200 mg/kg) to disrupt the normal bacterial flora. Cyclophosphamide was then administered to induce translocation of P. aeruginosa that had colonised the gastrointestinal tract so that gut-derived septicaemia was produced. In this model, intraperitoneal administration of antiserum against heat-killed bacteria, 100 microl/mouse, twice a day for 3 consecutive days significantly increased the survival rate over that of mice treated with normal rabbit serum. Antiserum against elastase, alkaline protease, or a combination of these two antisera, failed to provide significant protection. In contrast, antiserum against exotoxin A significantly increased the survival rate over that of mice treated with normal rabbit serum. These results indicate that passive immunisation with antiserum against heat-killed bacteria and exotoxin A, but not with antiserum against either elastase or alkaline protease, protects mice against gut-derived sepsis caused by P. aeruginosa.
Breather molecular complexes in a passively mode-locked fibre laser (2021)

Breathing solitons are nonlinear waves in which the energy concentrates in a localized and oscillatory fashion. Similarly to stationary solitons, breathers in dissipative systems can form stable bound states displaying molecule-like dynamics, which are frequently called breather molecules. So far, the experimental observation of optical breather molecules and the real-time detection of their dynamics have been limited to diatomic molecules, that is, bound states of only two breathers. In this work, we report on the observation of different types of breather complexes in a mode-locked fibre laser: multi-breather molecules, and molecular complexes originating from the binding of two breather-pair molecules or a breather pair molecule and a single breather. The inter-molecular temporal separation of the molecular complexes attains several hundreds of picoseconds, which is more than an order of magnitude larger than that of their stationary soliton counterparts and is a signature of long-range interactions. Numerical simulations of the laser model support our experimental findings. Moreover, non-equilibrium dynamics of breathing solitons are also observed, including breather collisions and annihilation. Our work opens the possibility of studying the dynamics of many-body systems in which breathers are the elementary constituents.
Bit Fusion: Bit-Level Dynamically Composable Architecture for Accelerating Deep Neural Networks (2017)

Fully realizing the potential of acceleration for Deep Neural Networks (DNNs) requires understanding and leveraging algorithmic properties. This paper builds upon the algorithmic insight that bitwidth of operations in DNNs can be reduced without compromising their classification accuracy. However, to prevent accuracy loss, the bitwidth varies significantly across DNNs and it may even be adjusted for each layer. Thus, a fixed-bitwidth accelerator would either offer limited benefits to accommodate the worst-case bitwidth requirements, or lead to a degradation in final accuracy. To alleviate these deficiencies, this work introduces dynamic bit-level fusion/decomposition as a new dimension in the design of DNN accelerators. We explore this dimension by designing Bit Fusion, a bit-flexible accelerator, that constitutes an array of bit-level processing elements that dynamically fuse to match the bitwidth of individual DNN layers. This flexibility in the architecture enables minimizing the computation and the communication at the finest granularity possible with no loss in accuracy. We evaluate the benefits of BitFusion using eight real-world feed-forward and recurrent DNNs. The proposed microarchitecture is implemented in Verilog and synthesized in 45 nm technology. Using the synthesis results and cycle accurate simulation, we compare the benefits of Bit Fusion to two state-of-the-art DNN accelerators, Eyeriss and Stripes. In the same area, frequency, and process technology, BitFusion offers 3.9x speedup and 5.1x energy savings over Eyeriss. Compared to Stripes, BitFusion provides 2.6x speedup and 3.9x energy reduction at 45 nm node when BitFusion area and frequency are set to those of Stripes. Scaling to GPU technology node of 16 nm, BitFusion almost matches the performance of a 250-Watt Titan Xp, which uses 8-bit vector instructions, while BitFusion merely consumes 895 milliwatts of power.
Increased tubular flow induces resetting of tubuloglomerular feedback in euvolemic rats (1996)

As single-nephron glomerular filtration rate (SNGFR) and late proximal flow (VLP) increase during growth or following volume expansion, the tubuloglomerular feedback (TGF) function (defined as the decrement in SNGFR due to the process of TGF) shifts rightward in the plane defined by VLP and SNGFR as required to maintain the homeostatic efficiency of TGF. It is not known whether this resetting of TGF requires changes in the systemic hormonal milieu or results from prolonged activation of TGF itself. We employed micropuncture and videometric flow velocitometry (an optical technique for measuring flow in unobstructed nephrons) to address this issue in Inactin-anesthetized euvolemic rats. The fractional compensation (C) of TGF for perturbations [late proximal flow perturbation (VH) = +/- 5 nl/min] in VLP was assessed repeatedly before and during a sustained increase in flow imposed by adding 20 nl/min to early proximal flow (VEP). Augmenting VEP initially saturated TGF, thus suppressing C. Over the next 30 min, C recovered to 70% of its original value, suggesting a rightward resetting of the TGF function to match the increase imposed on VLP. Resetting was confirmed by documenting an evolving asymmetry of C about VH = 0 by testing C vs. VH for -12 < or = VH < or = 12 in increments of 4 nl/min. Beyond 30 min of augmented VEP, C gradually declined due to desensitization of TGF. A sustained increase in VLP is sufficient to include TGF resetting, independent of any change in the systemic neurohumoral milieu.
Increased expression of the β1, α5 and αv integrin adhesion receptor subunits occurs coincident with remodeling of stress‐deprived rabbit anterior cruciate and medial collateral ligaments (1995)

The biomechanical, biochemical, and morphological properties of the anterior cruciate and medial collateral ligaments are dramatically altered in response to deprivation of normal physical forces and joint motion. Integrin adhesion receptors are known to play important roles in the tissue remodeling that occurs in the course of normal wound repair. We propose that integrins play a similar role in the remodeling of the extracellular matrix in stress-deprived periarticular ligaments. This study tests the hypothesis that altered expression of integrins on ligament fibroblasts accompanies this remodeling. The left knees of 15 New Zealand White rabbits were surgically immobilized in acute flexion and the right knees served as controls (no operation). The anterior cruciate and medial collateral ligaments were harvested at 1, 3, 5, 9, or 12 weeks after immobilization. Sections from the ligaments were immunostained with monoclonal antibodies specific for the integrin subunits beta 1, alpha 5, alpha 6, and alpha v, as well as with a negative control antibody. Fibroblasts within both the stress-deprived anterior cruciate and medial collateral ligaments demonstrated markedly increased staining for the beta 1, alpha 5, and alpha v subunits, as compared with the controls. The increased staining was greatest at 9 weeks in the anterior cruciate ligament and at 12 weeks in the medial collateral ligament. Western blot study of ligament proteins extracted with sodium dodecyl sulfate demonstrated an increased amount of beta 1 subunit protein in both ligaments from knees that were stress deprived for 9 and 12 weeks, as compared with the control ligaments.(ABSTRACT TRUNCATED AT 250 WORDS)
Using Change Point Analysis for Image Processing of Developing Adiabatic Two-Phase Flow After Expansion Valve (2007)

This paper presents a method of analyzing images obtained from a high speed camera in a study of developing two-phase refrigerant flow after an expansion valve, using a technique known as Change Point Analysis. Specifically, a method for automating the determination of separation distance and the location of the liquid/vapor interface in stratified type flows is outlined. The experiments were conducted in transparent PVC tubes, with inner diameters of 8.7mm and 15.3mm. A working fluid of oil free R134a was used. Dynamic flow parameters of inlet quality and mass flux were varied from 5–35% and 15–40g/s, respectively. Flow visualization was achieved through the use of a high speed camera.
Supramolecular synergy in the boundary lubrication of synovial joints (2015)

Hyaluronan, lubricin and phospholipids, molecules ubiquitous in synovial joints, such as hips and knees, have separately been invoked as the lubricants responsible for the remarkable lubrication of articular cartilage; but alone, these molecules cannot explain the extremely low friction at the high pressures of such joints. We find that surface-anchored hyaluronan molecules complex synergistically with phosphatidylcholine lipids present in joints to form a boundary lubricating layer, which, with coefficient of friction μ≈0.001 at pressures to over 100 atm, has a frictional behaviour resembling that of articular cartilage in the major joints. Our findings point to a scenario where each of the molecules has a different role but must act together with the others: hyaluronan, anchored at the outer surface of articular cartilage by lubricin molecules, complexes with joint phosphatidylcholines to provide the extreme lubrication of synovial joints via the hydration-lubrication mechanism.
High-level production of alpha-particle-emitting (211)At and preparation of (211)At-labeled antibodies for clinical use. (2001)

These results show that it is feasible to produce clinically relevant activities of (211)At-labeled antibodies and have permitted the initiation of a phase I trial of (211)At-labeled chimeric 81C6 administered directly into the tumor resection cavities of brain tumor patients.
Long-Acting Intranasal Insulin Detemir Improves Cognition for Adults with Mild Cognitive Impairment or Early-Stage Alzheimer's Disease Dementia (2015)

Previous trials have shown promising effects of intranasally administered insulin for adults with Alzheimer's disease dementia (AD) or amnestic mild cognitive impairment (MCI). These trials used regular insulin, which has a shorter half-life compared to long-lasting insulin analogues such as insulin detemir. The current trial examined whether intranasal insulin detemir improves cognition or daily functioning for adults with MCI or AD. Sixty adults diagnosed with MCI or mild to moderate AD received placebo (n = 20), 20 IU of insulin detemir (n = 21), or 40 IU of insulin detemir (n = 19) for 21 days, administered with a nasal drug delivery device. Results revealed a treatment effect for the memory composite for the 40 IU group compared with placebo (p < 0.05). This effect was moderated by APOE status (p < 0.05), reflecting improvement for APOE-ε4 carriers (p < 0.02), and worsening for non-carriers (p < 0.02). Higher insulin resistance at baseline predicted greater improvement with the 40 IU dose (r = 0.54, p < 0.02). Significant treatment effects were also apparent for verbal working memory (p < 0.03) and visuospatial working memory (p < 0.04), reflecting improvement for subjects who received the high dose of intranasal insulin detemir. No significant differences were found for daily functioning or executive functioning. In conclusion, daily treatment with 40 IU insulin detemir modulated cognition for adults with AD or MCI, with APOE-related differences in treatment response for the primary memory composite. Future research is needed to examine the mechanistic basis of APOE-related treatment differences, and to further assess the efficacy and safety of intranasal insulin detemir.
ChemInform Abstract: A Novel Transformation of 4‐Arylaminocoumarins to 6H‐1‐Benzopyrano‐(4,4‐b)quinolin‐6‐ones Under Vilsmeier‐Haack Conditions. (1987)

Abstract The 4‐aminocoumarins (III) are obtained from 4‐hydroxycoumarin (I) by reaction with the amines (II).
Dual axis operation of a micromachined rate gyroscope (2002)

Since micromachining technology hs raised the prospect of fabricating high performance sensors without the associated high cost and large size, many researchers have investigated micromachined rate gyroscopes. The vast majority of research has focused on single input axis rate gyroscopes, but this paper presents work on a dual input axis micromachined rate gyroscope. The key to successful simultaneous dual axis operation is the quad symmetry of the circular oscillating rotor design. Untuned gyroscopes with mismatched modes yielded random walk as low as 10/spl deg///spl radic/(Hour) with cross axis sensitivity ranging from 3% to 16%. Subsequent mode frequency matching via electrostatic tuning allowed performance of 2/spl deg///spl radic/(Hour), but at the expense of excessive cross axis sensitivity.
ADDRESSING POLICY CHALLENGES TO WOODY BIOPOWER PRODUCTION: SOCIAL ACCEPTANCE, BIOMASS CERTIFICATION AND LIMITED POLICY SUPPORT (2018)

Forestlands have been identified as a valuable resource to mitigate climate change due to the biome’s capacity to both sequester greenhouse gases and substitute for fossil fuels. Woody biomass has been proposed as a substitutable input for coalgenerated electricity as economies attempt to transition to renewable power while addressing economic development goals. However, increasing the intensity of forest management for energy production has the potential to result in significant ecological, economic and social consequences at local, regional and global scales. In this context, my dissertation explores the capacity of existing policy frameworks to stimulate and support sustainable power production from forest biomaterials. In Chapter Two, I explore the interactions between shifting goals, actors and institutions in influencing incentives that shape today’s policy mix for woody biopower production in Wisconsin. The study’s results reveal that the state’s shifting focus away from using renewable energy as a means to pursue climate change mitigation and energy security goals combined with an absence of supportive coalitions has resulted in the dismantling of support for the woody biopower policy framework. In Chapter Three, I use data from a household survey of Tomahawk, Wisconsin residents to evaluate support for woody biomass production for power generation. Results show that respondents in biomass producing communities are more supportive of biomass sources such as forestry residues and forestry thinnings than dedicated harvesting operations. In addition, the results indicate that using an xiii ecosystem services approach can help explain differences in support between these respondents and provide insights into socially acceptable forms of biomass harvesting operations. Chapter Four evaluates the use of sustainable forest management certification programs as a policy instrument to source sustainable woody biomass. The study evaluated the Forest Stewardship Council (FSC) and Sustainable Forestry Initiative (SFI) certification programs using bioenergy sustainability criteria found in the academic literature. The analysis shows a deficiency in these programs to address key criteria pertaining to climate change mitigation and would be improved by coupling sustainable forest management programs with bioenergy sustainability schemes such as designed by the Roundtable on Sustainable Biomaterials.