gebhart/patrepeval huggingface dataset:
In vitro Propagation and Genetic Homogeneity Assessment of Dendrobium crepidatum Lindley & Paxton (2022)

In vitro propagation is one of the most dependable methods to develop true-to-type plants for the commercial and conservation aspects. This study aims to produce the genetically identical plantlets of Dendrobium crepidatum Lindley and Paxton using in vitrodeveloped shoot explants. Coconut water (5 - 10%) supplemented Murashige and Skoog (MS) medium responded towards effective shoot formation with BAP (1.0 - 2.0 mg/l) and NAA (0.1 - 0.5 mg/l). NAA (0.5 - 1.0 mg/l) alone or in combination with BAP was found to be effective for root initiation in MS medium. Four RAPD (Random Amplified Polymorphic DNA) primers viz., G04, G10, G17 and G18 showed 100% genetic homogeneity in plants obtained from in vitro-developed shoot explant. However, G10, G17 and G18 RAPD primers showed 60, 25 and 100% genetic polymorphisms respectively in plants obtained from callus. Plants obtained from callus were genetically polymorphic where somaclonal variation might occur. This study reported the successful genetic homogeneity of shoot explant-derived plants of D. crepidatum. Hence, the present study has validated the establishment of standard in vitro protocol for significant propagation of superior genetically indistinguishable D. crepidatum for viable conservation and commercialization. Plant Tissue Cult. &amp; Biotech. 32(1): 1-11, 2022 (June)
Magnetic Iron Oxide Nanoparticles in 10−40 nm Range: Composition in Terms of Magnetite/Maghemite Ratio and Effect on the Magnetic Properties (2011)

Magnetic iron oxide nanoparticles in the 10−40 nm size range and with a reduced distribution in size have been synthesized under argon by using ammonium bases R4NOH (R = CH3, C2H5, C3H7) and a hydrothermal treatment. The size is tuned owing to the base to iron ratio and to the length of the alkyl chain R. We precipitate first ferric hydroxides at pH 1.5−2, then ferrous hydroxide at pH 5.5−6. The rapid increase of pH up to basic pH leads to the formation of magnetic iron oxide particles of 12 nm. For [base] to [Fe] ratio above 3.5, a homogeneous growth occurs during further hydrothermal treatment at 250 °C. The higher the quantity of base added and the longer the alkyl chain used, the smaller the particle size produced. For sizes above 20 nm, the Verwey transition at 120 K, characteristic of magnetite, is observed on the field cooling−zero field cooling magnetization curve. The nanoparticles can be described by a core−shell model, that is, a magnetite core surrounded by an oxidized layer close to maghemite. The fractional volume of maghemite increases as the particle size decreases so that below 20 nm, nanoparticles cannot be properly labeled as “magnetite”.
Interleukin 11 advances progesterone-induced decidualization of human endometrial stromal cells (2002)

Differentiation of endometrial stromal cells into decidual cells is crucial for embryo implantation and placentation. Interleukin (IL)-11 signalling is essential for adequate decidualization in the mouse uterus. We examined the role of IL-11 during progesterone-induced decidualization of human endometrial stromal cells over a 10-12 day period, using prolactin (PRL) production as a decidual marker. These cells produced biologically active IL-11 and expressed IL-11, IL-11Ralpha and PRL mRNA during decidualization. Neutralization of endogenous IL-11 with an anti-human (hu)IL-11 antibody (AB) reduced production of PRL from day 8 and insulin-like growth factor binding protein (IGFBP)-1, another marker of decidualization, from day 10 of culture. Following AB washout, PRL and IGFBP-1 secretion increased. Addition of recombinant (r)huIL-11 (10 or 100 ng/ml) to endometrial stromal cells increased secretion of PRL from day 4 and IGFBP-1 from day 6 compared with progesterone alone. Morphological signs of differentiation accompanied biochemical differentiation in the progesterone-treated cells and were further induced by exogenous rhuIL-11. Our observations demonstrate that human endometrial stromal cells produce biologically active IL-11, which promotes progesterone-induced decidualization. These results suggest that IL-11 has both paracrine and autocrine actions on human endometrial stromal cells and plays an important role in preparing the human endometrium for implantation.
Image Matching Assisted Phase-Sensitive Optical Time Domain Reflectometry (2018)

Image matching algorithms are proposed to improve the temporal resolution of dualfrequency phase-sensitive OTDR. Experimental results show that the temporal resolution is improved by six times compared with 1D cross-covariation under the same precision.
Refinements of nerve repair with connector‐assisted coaptation (2016)

Peripheral nerve repair can be very rewarding for both surgeon and patient when expected outcomes are achieved. In many cases, however, the results are suboptimal due to number of possible objective and technical reasons. Although we cannot influence patent's comorbidities, the extent or mechanism of the nerve injury, we may help optimize technical details when aiming towards improved outcomes. While the suture coaptation for primary nerve repair or nerve reconstruction with grafting served as the reconstructive standard for many decades, technical imperfections remain threats to reconstructive goals. Tension, fascicular misalignment generated by over tightening suture coaptation, deeply placed sutures, reactive scarring to foreign material at anastomosis site, may all negatively affect axonal regeneration. As the goal of every nerve repair is to have ideally opposed tension free nerve fascicles, protected from the deleterious effects of the wound bed. The utilization of coaptation aids to overcome the challenges of nerve repair has been suggested as an alternative to the classical suture repair. A review of clinical literature was performed to assess the evidence for this technique and the critical success factors to consider when implementing this technique. Twelve clinical studies met criteria, majority suggesting improved outcomes by the utilization of a coaptation aid. Most commonly reported improvements were improved sensory outcomes, reduced tenderness or pain at the coaptation site and reduced operative time. The current clinical evidence data suggests that utilization of a coaptation aid is a technical innovation to help provide better nerve repair and reconstructive functional outcomes.
A Pilot Trial of Molecularly Tailored Therapy for Patients with Metastatic Pancreatic Ductal Adenocarcinoma (2019)

<b>Purpose:</b> Despite the wide adoption of tumor molecular profiling, there is a dearth of evidence linking molecular biomarkers for treatment selection to prediction of treatment outcomes in patients with metastatic pancreatic cancer. We initiated a pilot study to test the feasibility of designing a larger phase II trial of molecularly tailored treatment for metastatic pancreatic cancer. <b>Methods:</b> Our study aimed to assess the feasibility of following a treatment algorithm based on the expression of three published predictive markers of response to chemotherapy: ribonucleotide reductase catalytic subunit M1 (for gemcitabine); excision repair cross-complementation group 1 (for platinum agents); and thymidylate synthase (for 5-fluorouracil) in patients with untreated, metastatic pancreatic cancer. Results of the tumor biopsy analysis were used to assign patients to one of seven doublet regimens. Key secondary objectives included response rate (RR), disease control rate (DCR), progression-free survival (PFS), and overall survival (OS). <b>Results:</b> Between December 2012 and March 2015, 30 patients were enrolled into the study. Ten patients failed screening primarily due to inadequate tumor tissue availability. Of the remaining 20 patients, 19 were assigned into 6 different chemotherapy doublets, and achieved an RR of 28%, with a DCR rate of 78%. The median PFS and OS were 5.78 and 8.21 months, respectively. <b>Conclusions:</b> The incorporation of biomarkers into a treatment algorithm is feasible and resulted in a PFS and OS similar to other doublet therapies for patients with metastatic pancreatic cancer. Based on the results from this pilot study, a larger phase II randomized trial of molecularly targeted therapy versus physicians' choice of standard of care has been initiated in the second-line setting (NCT02967770).
Anesthesia and Multiorgan Protein Remodeling (2004)

We thank Dr. Heerdt for his comments, which support our hypothesis of persisting effects of volatile anesthetics after the end of anesthesia. However, the approaches chosen by Heerdt et al. and our group were widely divergent.Heerdt et al. 1found changes in the protein expression of the sarcoplasmatic endoreticular calcium adenosine triphosphatase subtype 2a in rabbit hearts after 1, 3, and 5 h of halothane anesthesia. This study differs from ours2with respect to animals (rabbits vs. rats), volatile anesthetic (halothane vs. desflurane), target organ (heart vs. brain), and minimum alveolar concentration (0.7 vs. 1.0). Another major difference is the killing time, which was immediately after 1, 3, or 5 h of anesthesia in the study of Heerdt et al. , whereas it was either immediately or 24 or 72 h after 3 h of anesthesia in our study. These different experimental approaches make it difficult to compare both studies directly, although the line of evidence is similar. Interestingly, both findings may be based on a common principle of interaction between drug and protein expression, challenging the current hypothesis of a complete reversibility of the effects of volatile anesthetics.Because of our strict statistical prerequisites, we cannot exclude that a homologue of sarcoplasmatic endoreticular calcium adenosine triphosphatase subtype 2a is also differentially expressed in the brain. Moreover, the technical limits of our approach did not allow us to investigate integral membrane proteins such as sarcoplasmatic endoreticular calcium adenosine triphosphatase subtype 2a, because our protocol isolates cytosolic proteins.Therefore, our current approach follows the line suggested in the editorial by Dr. Hogan.3Based on our previous exciting findings regarding desflurane anesthesia in the brain, we are currently investigating further volatile anesthetics (i.e. , sevoflurane and isoflurane) in different organs (i.e. , heart, liver, and kidney) with respect to changes in protein expression profiles of specific tissues.* Faculty of Clinical Medicine Mannheim, Mannheim, Germany. c.fuetterer@pio1.uni-heidelberg.de
Comparison and Validation of Drug Loading Parameters of PEGylated Nanoparticles Purified by a Diafiltration Centrifugal Device and Tangential Flow Filtration (2008)

This article describes drug loading validation of nanoparticles. Ultracentrifugation was avoided because of problems arising from small-sized particles. Ultrafiltration was adopted in two different modes followed by monitoring of polyvinyl alcohol (PVA), dextran sulfate (DS), and loperamide HCl contents. Diafiltration centrifugation removed all PVA at the fourth cycle and provided significantly (p = .000, .017) higher drug loading values compared with tangential flow filtration (TFF). This was due to residual PVA associated with the nanoparticles. TFF enabled satisfactory dry weight recovery (101.66 ± 4.45%, n = 3) of nanoparticles during extended purification. Indirect drug loading (from the purification curve) was not significantly different (p = .450, .487) to the direct drug loading values. Encapsulation parameters were obtained from the purification curve once quantitative estimation of the all formulation components was established.
Novel active FET circular patch antenna arrays for quasi-optical power combining (1994)

This paper discusses two-dimensional planar arrays of weakly coupled active circular patch antennas suitable for quasi-optical power combiners. A novel radiating element that has low cross-polarization is described. A two-dimensional array analysis was developed to address radiation pattern and phase problems. Equivalent isotropic radiated power levels of 1.5 W for a two by two FET array and 3.8 W for a two by four FET array have been obtained at X-band.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The Collapse and Expansion of Liquid-Filled Elastic Channels and Cracks (2015)

The rate at which fluid drains from a collapsing channel or crack depends on the interaction between the elastic properties of the solid and the fluid flow. The same interaction controls the rate at which a pressurized fluid can flow into a crack. In this paper, we present an analysis for the interaction between the viscous flow and the elastic field associated with an expanding or collapsing fluid-filled channel. We first examine an axisymmetric problem for which a completely analytical solution can be developed. A thick-walled elastic cylinder is opened by external surface tractions, and its core is filled by a fluid. When the applied tractions are relaxed, a hydrostatic pressure gradient drives the fluid to the mouth of the cylinder. The relationship between the change in dimensions, time, and position along the cylinder is given by the diffusion equation, with the diffusion coefficient being dependent on the modulus of the substrate, the viscosity of the fluid, and the ratio of the core radius to the exterior radius of the cylinder. The second part of the paper examines the collapse of elliptical channels with arbitrary aspect ratios, so as to model the behavior of fluid-filled cracks. The channels are opened by a uniaxial tension parallel to their minor axes, filled with a fluid, and then allowed to collapse. The form of the analysis follows that of the axisymmetric calculations, but is complicated by the fact that the aspect ratio of the ellipse changes in response to the local pressure. Approximate analytical solutions in the form of the diffusion equation can be found for small aspect ratios. Numerical solutions are given for more extreme aspect ratios, such as those appropriate for cracks. Of particular note is that, for a given cross-sectional area, the rate of collapse is slower for larger aspect ratios. With minor modifications to the initial conditions and the boundary conditions, the analysis is also valid for cracks being opened by a pressurized fluid.
The New Publishing Landscape: In Conversation with Iain Sarjeant of Another Place Press (2017)

Iain Sarjeant is a photographer based in the Highlands of Scotland who founded the Another Place Press as a small independent publisher to showcase contemporary landscape photography. To date he has published nine books that explore landscape covering themes documenting the land, place, journey, city and environment from the remotest corners of the globe to the centre of the largest cities. It’s an ambitious premise and even more so as the imprint consists of just one employee. Iain is that employee and his responsibilities include all aspects of publishing the books, running the blog, all social media and continuing to work as a commissioned photographer as well as progressing his personal projects. Despite this he spared sometime to talk with Grant Scott about the current independent publishing landscape and his personal experiences in establishing his own publishing company.
Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment (2001)

Because human CD34+ and murine Sca-1+ hematopoietic stem-progenitor cells (HSPCs) express platelet-binding sialomucin P-selectin (CD162) and integrin Mac-1 (CD11b-CD18) antigen, it was inferred that these cells might interact with platelets. As a result of this interaction, microparticles derived from platelets (PMPs) may transfer many platelet antigens (CD41, CD61, CD62, CXCR4, PAR-1) to the surfaces of HSPCs. To determine the biologic significance of the presence of PMPs on human CD34+ and murine Sca-1+ cells, their expressions on mobilized peripheral blood (mPB) and on nonmobilized PB- and bone marrow (BM)-derived CD34+ cells were compared. In addition, the effects of PMPs on the proliferation of CD34+ and Sca-1+ cells and on adhesion of HSPCs to endothelium and immobilized SDF-1 were studied. Finally, the hematopoietic reconstitution of lethally irradiated mice receiving transplanted BM mononuclear cells covered or not covered with PMPs was examined. It was found that PMPs are more numerous on mPB than on BM CD34+ cells, do not affect the clonogenicity of human and murine HSPCs, and increase adhesion of these cells to endothelium and immobilized SDF-1. Moreover, murine BM cells covered with PMPs engrafted lethally irradiated mice significantly faster than those not covered, indicating that PMPs play an important role in the homing of HSPCs. This could explain why in a clinical setting human mPB HSPCs (densely covered with PMPs) engraft more rapidly than BM HSPCs (covered with fewer PMPs). These findings indicate a new role for PMPs in stem cell transplantation and may have clinical implications for the optimization of transplantations.
The fiber reorientation problem revisited in the context of Eshelbian micromechanics: theory and computations (2015)

Abstract A multiscale micromechanical model for soft tissue is presented. This model accounts for the progressive stiffening of the mechanical response due to load‐induced microstructural rearrangements of the fibers within the microstructure. (© 2015 Wiley‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
The Visual Decision Maker - A Movie Recommender for Co-Located Users (2002)

We present the Visual Decision Maker (VDM), an application that gives movie recommendations to groups of people sitting together. The VDM provides a TV like user experience: a stream of movie stills flows towards the center of the screen, and users press buttons on remote controls to vote on the currently selected movie. A collaborative filtering engine provides recommendations for each user and for the group as a whole based on the votes. Three principles guided our design of the VDM: shared focus, dynamic pacing, and encouraging conversations. In this paper we present the results of a four month public installation and a lab study showing how these design choices affected people’s usage and people’s experience of the VDM. Our results show that shared focus is important for users to feel that the group’s tastes are represented in the recommendations.
Increased pyruvate dehydrogenase kinase activity in response to sepsis (1991)

The effect of sterile inflammation and sepsis on the proportion of active pyruvate dehydrogenase complex (PDH) in mitochondria isolated from skeletal muscle has been investigated. The proportion of active PDH in mitochondria isolated from septic animals was significantly reduced compared with control under all incubation conditions examined, even in the presence of inhibitors of the PDH kinase. There was no significant difference between control and sterile inflammation in any of the incubations examined. The rate constant for ATP-dependent inactivation of the PDH complex in mitochondrial extracts from control animals was -0.42 min-1 (r = 0.993; P less than 0.001) and was not altered in mitochondrial extracts from sterile inflammatory animals (-0.43 min-1; r = 0.999; P less than 0.001). However, rate constants for inactivation in septic animals was significantly increased over twofold to -1.08 min-1 (r = 0.987; P less than 0.001) (P less than 0.001 vs. control or sterile inflammation). In the presence of inhibitors of the PDH kinase reaction (2.5 mM pyruvate or 1 mM dichloroacetate), inactivation of PDH after addition of ATP was significantly greater in mitochondrial extracts from septic than either control or sterile inflammatory animals. These results suggest that sepsis, but not sterile inflammation, induces a stable factor in skeletal muscle mitochondria that increased PDH kinase activity.
Simplified design of rock cavern concrete lining to resist shock loading (2010)

一个简化方法对装载的吃惊在地下的岩石大洞 / 隧道为具体衬里的设计被建议。装载可以在地面表面或邻近大洞 / 隧道的爆炸的地面穿入射弹上源于炸药的爆炸。产生问题要求一致地在跨度上散布的一个短暂压力装载的一根横梁的动力学的答案。根据有岩石门栓和 shotcrete 的系统的机械特征，一个动态支持设计方法基于自由(SDOF ) 的相等的单个度被提出。SDOF 方法被用于获得最大的排水量在横梁中间跨度，它经常是在强风抵抗的设计的控制因素。在这个问题的明确的表达，建议方法联合 spalling 和结构的动力学理论的现象。一个例子被提供表明这个简化方法的适用性。
Results of Catheter Ablation of Ventricular Tachycardia Using Direct Current Shocks (1989)

Thirty-three patients with recurrent ventricular tachycardia (VT) underwent catheter ablation with direct-current shocks. One to four shocks of 100-300 joules were delivered to the presumed VT exit sites as identified by endocardial mapping and pace mapping. Fifteen patients (45%) had no recurrence of symptomatic VT during a follow-up interval of 15.5 +/- 10 months (mean +/- standard deviation). Five patients experienced six nonfatal complications (new VT or ventricular fibrillation, transient neurological deficit, atrioventricular block, brachial artery thrombosis). In conclusion, catheter ablation in selected patients with recurrent VT has the potential for preventing recurrences of VT over the long-term and is relatively safe.
A Novel Cascade Benzyne Nucleophilic Addition/Fries Rearrangement for Entry into 2,3-Disubstituted Phenols (2017)

Correction to: A Novel Cascade Benzyne Nucleophilic Addition/Fries Rearrangement for Entry into 2,3-Disubstituted PhenolsSynlett eFirstDOI: 10.1055/s-0036-1588938
Visualization of an acoustic stationary wave by optical feedback interferometry (2018)

This paper presents an experimental technique for two-dimensional imaging of dynamic acoustic pressure changes that is applied to visualize a stationary acoustic wave. This technique uses the optical feedback interferometry sensing scheme with a near-infrared laser diode and a two-axis scanning system. The stationary acoustic wave is generated by using a 40 kHz piezoelectric transducer pointing toward a concave acoustic reflector. The acoustic pressure dynamic changes are measured due to its impact on the propagating medium refractive index, which variation is integrated along the laser optical path from the laser diode to a distant mirror and back. The imaging system records a 100×50 pixels image of the acoustic pressure in 66 min.
ChemInform Abstract: Synthesis of Benzothiophene Analogues of Ketoconazole and Itraconazole. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.