gebhart/patrepeval huggingface dataset:
Achievable Rates for $K$-User Gaussian Interference Channels (2012)

The aim of this paper is to study the achievable rates for a K-user Gaussian interference channel (G-IFC) for any signal-to-noise ratio using a combination of lattice and algebraic codes. Lattice codes are first used to transform the G-IFC into a discrete input--output noiseless channel, and subsequently algebraic codes are developed to achieve good rates over this new alphabet. In this context, a quantity called efficiency is introduced which reflects the effectiveness of the algebraic coding strategy. This paper first addresses the problem of finding high-efficiency algebraic codes. A combination of these codes with Construction-A lattices is then used to achieve nontrivial rates for the original G-IFC.
ChemInform Abstract: Polyuronide Interactions with Polycoordinative Metal Ions (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Proteomics technology - a powerful tool for the biomedical scientists. (2013)

"Proteomics" refers to the systematic analysis of proteins. It complements other "omics" technologies such as genomics and transcriptomics in elucidating the identity of proteins of an organism, and understanding their functions. Proteomics is used in many areas of research such as discovery of markers for diagnosis and vaccine candidates, understanding pathogenic mechanisms, in the study of expression patterns at different time points and in response to different stimuli, and in elucidating functional protein networks. Proteomics analysis involves sample preparation, protein separation, and protein identification. The 'heart' of current proteomics is mass-spectrometry, with LC-MS/MS and MALDI-TOF/TOF being commonly used equipment. However, the high costs of the equipment, software, databases, and the need for skilled personnel limit the wide utilization of this technology in the less developed countries. Therefore, there need to be sharing of facilities, better networking and collaborations among our scientists and laboratories to take advantage of this powerful technology.
5-Alkoxy(Aralkoxy)-3,6-dihydro-2H-1,3,4-oxadiazin-2-one(thione) aus 2-Hydroxycarbonsäure-hydrazonid-estern / 5-Alkoxy(Aralkoxy)-3,6-dihydro-2H-1,3,4-oxadiazin-2-ones(thiones) from 2-Hydroxycarbohydrazono Esters (1994)

Abstract The sulfhydrolysis of the glycolimidates 3 affords 2-hydroxy thiocarboxylic O-esters (4), which on treatment with phenylhydrazine give glycolohydrazonates of type 5. Under suitable conditions the reaction of 5 with phosgene or 1,1′-thiocarbonyldiimidazole produces 3,6-dihydro-2H -1,3,4-oxadiazin-2-ones(thiones) 6 as stable compounds.
Classification of voltage sags causes in industrial power networks using multivariate time‐series (2023)

Abstract Voltage sags are the most frequent and impactful disturbances in industrial power grids, leading to high financial losses for industrial clients. The identification of the cause and its relative location is crucial for the contractual relation between the energy provider and the industrial customers. This paper proposes a methodology to identify the origins of voltage sags based on instantaneous symmetrical components and dynamic time warping. Short‐Time Fourier and Fortescue transform are implemented in the pre‐processing step using the voltage and current waveforms. Then, a distance‐based classification strategy to identify the sources of voltage sags is used. It relies on a four‐dimension time‐series signature used as features. Moreover, a confidence index associated with the classification output is provided. The proposal offers an easy implementation in industrial applications with no previous recorded data. It has the benefit of using a reduced‐size reference database entirely composed of synthetic data. The main advantages of the proposed method are its generalization capabilities and the possibility of raising an alert based on the confidence index. The obtained classification accuracy on synthetic data with seven causes is 100%. The method reaches a classification F1‐score higher than 99% with field measurements representing five classes obtained from three different industrial sites.
Bone structure and remodelling in stroke patients: Early effects of zoledronate (2008)

Stroke patients had higher resorption indices and lower bone forming surfaces than controls, consistent with uncoupling of bone remodelling. These findings are preliminary and a larger study is required to evaluate the contributions of gender, age and hemiplegic status to the remodelling imbalance. Zoledronate therapy was associated with a reduction in osteoclastic cell numbers consistent with its known mode of action in bone.
Penicillium expansum Volatiles Reduce Pine Weevil Attraction to Host Plants (2013)

The pine weevil Hylobius abietis (L.) is a severe pest of conifer seedlings in reforested areas of Europe and Asia. To identify minimally toxic and ecologically sustainable compounds for protecting newly planted seedlings, we evaluated the volatile metabolites produced by microbes isolated from H. abietis feces and frass. Female weevils deposit feces and chew bark at oviposition sites, presumably thus protecting eggs from feeding conspecifics. We hypothesize that microbes present in feces/frass are responsible for producing compounds that deter weevils. Here, we describe the isolation of a fungus from feces and frass of H. abietis and the biological activity of its volatile metabolites. The fungus was identified by morphological and molecular methods as Penicillium expansum Link ex. Thom. It was cultured on sterilized H. abietis frass medium in glass flasks, and volatiles were collected by SPME and analyzed by GC-MS. The major volatiles of the fungus were styrene and 3-methylanisole. The nutrient conditions for maximum production of styrene and 3-methylanisole were examined. Large quantities of styrene were produced when the fungus was cultured on grated pine bark with yeast extract. In a multi-choice arena test, styrene significantly reduced male and female pine weevils' attraction to cut pieces of Scots pine twigs, whereas 3-methylanisole only reduced male weevil attraction to pine twigs. These studies suggest that metabolites produced by microbes may be useful as compounds for controlling insects, and could serve as sustainable alternatives to synthetic insecticides.
GDNF rescues hyperglycemia-induced diabetic enteric neuropathy through activation of the PI3K/Akt pathway (2006)

Diabetes can result in loss of enteric neurons and subsequent gastrointestinal complications. The mechanism of enteric neuronal loss in diabetes is not known. We examined the effects of hyperglycemia on enteric neuronal survival and the effects of glial cell line-derived neurotrophic factor (GDNF) on modulating this survival. Exposure of primary enteric neurons to 20 mM glucose (hyperglycemia) for 24 hours resulted in a significant increase in apoptosis compared with 5 mM glucose (normoglycemia). Exposure to 20 mM glucose resulted in decreased Akt phosphorylation and enhanced nuclear translocation of forkhead box O3a (FOXO3a). Treatment of enteric neurons with GDNF ameliorated these changes. In streptozotocin-induced diabetic mice, there was evidence of myenteric neuronal apoptosis and reduced Akt phosphorylation. Diabetic mice had loss of NADPH diaphorase-stained myenteric neurons, delayed gastric emptying, and increased intestinal transit time. The pathophysiological effects of hyperglycemia (apoptosis, reduced Akt phosphorylation, loss of inhibitory neurons, motility changes) were reversed in diabetic glial fibrillary acidic protein-GDNF (GFAP-GDNF) Tg mice. In conclusion, we demonstrate that hyperglycemia induces neuronal loss through a reduction in Akt-mediated survival signaling and that these effects are reversed by GDNF. GDNF may be a potential therapeutic target for the gastrointestinal motility disorders related to diabetes.
295 mW output, frequency-stabilized erbium silica fiber laser with a linewidth of 5 kHz and a RIN of −120 dB/Hz (2016)

We demonstrate the frequency stabilization of a high output power, erbium silica fiber laser by utilizing a (13)C2H2 (acetylene) absorption line at 1538.8 nm and a H(13)C(14)N (hydrogen cyanide) absorption line at 1549.73 nm. We introduced a novel short ring cavity configuration and pump power feedback control to suppress the intensity noise of the laser output, which is caused by the relaxation oscillation of erbium ions. As a result, we succeeded in simultaneously obtaining a stable single-frequency oscillation with an output power of over 290 mW, a linewidth of 5 kHz, and a low relative intensity noise (RIN) of -120 dB/Hz. The frequency stabilities reached 2.8 × 10(-11) and 6.9 × 10(-11) for an integration time of 1 s with a (13)C2H2 and a H(13)C(14)N absorption line, respectively.
Experiment Document for 01-E077 Microgravity Investigation of Crew Reactions in 0-G (MICRO-G) (2003)

The Experiment Document (ED) serves the following purposes: a) It provides a vehicle for Principal Investigators (PIS) to formally specify the requirements for performing their experiments. b) It provides a technical Statement of Work (SOW). c) It provides experiment investigators and hardware developers with a convenient source of information about Human Life Sciences (HLS) requirements for the development and/or integration of flight experiment projects. d) It is the primary source of experiment specifications for the HLS Research Program Office (RPO). Inputs from this document will be placed into a controlled database that will be used to generate other documents.
Dual-Function Reporter Protein for Analysis of Gene Expression in Living Cells (1998)

The firefly luciferase (Luc) protein and the jellyfish green fluorescent protein (GFP) are two commonly used molecular reporters that can be detected noninvasively in living cells. The properties that make GFP or Luc useful for a particular experimental application are quite distinct. A recombinant protein with both fluorescent and bioluminescent characteristics might take advantage of the strengths of both reporters. An expression vector encoding a chimeric protein in which GFP was tethered to Luc through a 19-amino acid linker was prepared and characterized. Western blotting with antibodies specific for either GFP or Luc showed that a protein of appropriate size was expressed in transfected cells. Fluorescence microscopy revealed bright green fluorescence from transfected cells, indicating proper formation of the GFP chromophore. Luc enzymatic activity in protein extracts from transfected cells showed that Luc was fully functional. The treatment of living cell cultures stably expressing the GFP-Luc fusion protein with the protein translation-inhibitor cycloheximide (Chx) was used to show that the half-life for Luc protein activity was approximately 2 h at 37 degrees C. The utility of this dual-function reporter protein was shown by the identification of single living cells expressing the chimeric protein within a population by fluorescence microscopy, followed by quantification of Luc activity from the same living cells.
Precoded OFDM - an idea whose time has come (2004)

This paper discusses the effect of spectral nulls on conventional OFDM systems. Analytical and simulated results are provided for both uncoded unprecoded OFDM and uncoded precoded OFDM. Although known about for some time, orthogonal precoders for OFDM have largely been ignored in the literature and have not been incorporated into any major standards. It is demonstrated how previously unreported orthogonal, channel-independent precoders provide a dramatic improvement to the BER of conventional OFDM systems. We explain how orthogonal precoders achieve improved BER for OFDM systems and propose the use of a number of transforms not previously considered.
The Transcriptional Activator Hypoxia Inducible Factor 2 (HIF-2/EPAS-1) Regulates the Oxygen-Dependent Expression of Erythropoietin in Cortical Astrocytes (2006)

In the ischemic or hypoxic brain, astrocytes appear to be one of the main sources of erythropoietin (EPO). In this study, we investigated the differential contribution of hypoxia inducible factor (HIF) isoforms to the regulation of hypoxic EPO expression in cultured astrocytes. In addition, using an in vitro model of oxygen-glucose deprivation (OGD), we studied the role of HIF-1alpha and HIF-2alpha in the generation of paracrine protective signals by astrocytes that modulate the survival of neurons exposed to OGD. Expression of HIF-1alpha or HIF-2alpha was abrogated by infecting astrocytes with lentiviral particles encoding small interference RNA specific for HIF-1alpha or HIF-2alpha (siHIF-1alpha or siHIF-2alpha). Astrocytes infected with siHIF-1alpha showed abrogated hypoxic induction of vascular endothelial growth factor (VEGF) and lactate dehydrogenase (LDH) but normal EPO induction. In contrast, reduction of HIF-2alpha expression by siHIF-2alpha led to a drastic decrease of EPO hypoxic expression, but it did not affect LDH or VEGF upregulation. To further test whether HIF-2 is sufficient to drive EPO upregulation, we expressed oxygen-insensitive mutant forms of HIF-1alpha (mtHIF-1alpha) (P402A/P577A) and HIF-2alpha (mtHIF-2alpha) (P405A/P530A). Expression of mtHIF-2alpha but not mtHIF-1alpha in normoxic astrocytes resulted in a significant upregulation of EPO mRNA and protein. Accordingly, HIF-2alpha but not HIF-1alpha was found to be associated with the EPO hypoxia-response element by a chromatin immunoprecipitation assay. Interestingly, conditioned medium from astrocytes challenged by sublethal OGD improved neuronal survival to OGD; however, this effect was abolished during the downregulation of astrocytic HIF-2alpha using siHIF-2alpha. These results indicate that HIF-2alpha mediates the transcriptional activation of EPO expression in astrocytes, and this pathway may promote astrocytic paracrine-dependent neuronal survival during ischemia.
Stem Cell Properties in Cell Cultures From Different Stage of Melanoma Progression (2013)

Cutaneous melanoma is an extremely heterogenous human cancer. The most aggressive melanoma may contain deregulated cells with undifferentiated/stem cell-like phenotype. A critical mechanism by which melanoma cells enhance their invasive capacity is the dissolution of the intercellular adhesion and the acquisition of mesenchymal features as a part of an epithelial-to-mesenchymal transition. The aim of this study was to clarify the role of a stem cell-like population in human melanomas by means of melanocytic cell culture analysis obtained from distinct histotypes of primary and metastatic malignant melanoma. Patients with advanced melanoma >2 cm in diameter and/or >300 mm surface were enrolled. The melanoma cells were isolated from skin biopsies of lentigo maligna melanoma, superficial spreading melanoma, nodular melanoma, and metastatic melanoma. The colony forming unit assay and alkaline phosphatase stain were evaluated. Cells were subsequently cultured and maintained in different media to evaluate their ability to differentiate into osteogenic and adipogenic lineages. Immunohistochemistry and flow cytometry analysis were performed to evaluate antigenic markers CD90, CD73, CD105, CD146, CD20, CD166, and Nestin. This study confirms that melanoma can include heterogenous cell populations with the ability both to self-renew and to a give rise to differentiated progeny. Melanoma cells displayed intratumoral heterogeneity and dynamic antigen phenotypes. Histologically, transitions from normal skin to melanoma were associated with a gradual increase in the expression of CD146, CD20, CD133, Nestin, and CD73. These molecular profiles could be further analyzed and, in the future, used for the development of novel biomolecular targeted-therapy approaches.
Myocardin and myocardin-related transcription factor-A synergistically mediate actin cytoskeletal-dependent inhibition of liver fibrogenesis (2020)

Activation of hepatic stellate cells (HSCs), characterized by development of a robust actin cytoskeleton and expression of abundant extracellular matrix (ECM) proteins, such as type 1 collagen (COL.1), is a central cellular and molecular event in liver fibrosis. It has been demonstrated that HSCs express both myocardin and myocardin-related transcription factor-A (MRTF-A). However, the biological effects of myocardin and MRTF-A on HSC activation and liver fibrosis, as well as the molecular mechanism under the process, remain unclear. Here, we report that myocardin and MRTF-A's expression and nuclear accumulation are prominently increased during the HSC activation process, accompanied by robust activation of actin cytoskeleton dynamics. Targeting myocardin and MRTF-A binding and function with a novel small molecule, CCG-203971, led to dose-dependent inhibition of HSC actin cytoskeleton dynamics and abrogated multiple functional features of HSC activation (i.e., HSC contraction, migration and proliferation) and decreased COL.1 expression in vitro and liver fibrosis in vivo. Mechanistically, blocking the myocardin and MRTF-A nuclear translocation pathway with CCG-203971 directly inhibited myocardin/MRTF-A-mediated serum response factor (SRF), and Smad2/3 activation in the COL.1α2 promoter and indirectly abrogated actin cytoskeleton-dependent regulation of Smad2/3 and Erk1/2 phosphorylation and their nuclear accumulation. Finally, there was no effect of CCG-203971 on markers of inflammation, suggesting a direct effect of the compound on HSCs and liver fibrosis. These data reveal that myocardin and MRTF-A are two important cotranscriptional factors in HSCs and represent entirely novel therapeutic pathways that might be targeted to treat liver fibrosis.<b>NEW & NOTEWORTHY</b> Myocardin and myocardin-related transcription factor-A (MRTF-A) are upregulated in activated hepatic stellate cells (HSCs) in vitro and in vivo, closely associated with robustly increased actin cytoskeleton remodeling. Targeting myocardin and MRTF-A by CCG-203971 leads to actin cytoskeleton-dependent inhibition of HSC activation, reduced cell contractility, impeded cell migration and proliferation, and decreased COL.1 expression in vitro and in vivo. Dual expression of myocardin and MRTF-A in HSCs may represent novel therapeutic targets in liver fibrosis.
Microwave-Assisted γ-Valerolactone Production for Biomass Lignin Extraction: A Cascade Protocol (2016)

The general need to slow the depletion of fossil resources and reduce carbon footprints has led to tremendous effort being invested in creating "greener" industrial processes and developing alternative means to produce fuels and synthesize platform chemicals. This work aims to design a microwave-assisted cascade process for a full biomass valorisation cycle. GVL (γ-valerolactone), a renewable green solvent, has been used in aqueous acidic solution to achieve complete biomass lignin extraction. After lignin precipitation, the levulinic acid (LA)-rich organic fraction was hydrogenated, which regenerated the starting solvent for further biomass delignification. This process does not requires a purification step because GVL plays the dual role of solvent and product, while the reagent (LA) is a product of biomass delignification. In summary, this bio-refinery approach to lignin extraction is a cascade protocol in which the solvent loss is integrated into the conversion cycle, leading to simplified methods for biomass valorisation.
NCAM POLYSIALIC ACID (1999)

Polysialic acid (PSA) was discovered as a major carbohydrate component of vertebrate brains largely on the basis of its unusual composition and size (Finne, 1982). As a linear homopolymer (n=8 to over 100) of alpha-2,8-linked sialic, PSA is a remarkably simple yet unique structure (Figure 1) (Finne and Makela, 1985; Livingston et al., 1988). This unique conformation allows for two very specific tools in the study of PSA, monoclonal antibodies (Acheson et al., 1991; Frosch et al., 198 5) and a fortuitous bacteriophagederived enzyme, endoneuraminidase (endo N) (Vimr et al., 1984). The value of this enzyme lies in the fact that it does not alter any other known sialic acid-containing structure (Hallenbeck et al., 1987) and is suitable for both in vitro and in vivo studies (Rutishauser et al., 1985).
Combinatorial tissue engineering partially restores function after spinal cord injury (2019)

Hydrogel scaffolds provide a beneficial microenvironment in transected rat spinal cord. A combinatorial biomaterials-based strategy provided a microenvironment that facilitated regeneration while reducing foreign body reaction to the three-dimensional spinal cord construct. We used poly lactic-co-glycolic acid microspheres to provide sustained release of rapamycin from Schwann cell (SC)-loaded, positively charged oligo-polyethylene glycol fumarate scaffolds. The biological activity and dose-release characteristics of rapamycin from microspheres alone and from microspheres embedded in the scaffold were determined in vitro. Three dose formulations of rapamycin were compared with controls in 53 rats. We observed a dose-dependent reduction in the fibrotic reaction to the scaffold and improved functional recovery over 6 weeks. Recovery was replicated in a second cohort of 28 animals that included retransection injury. Immunohistochemical and stereological analysis demonstrated that blood vessel number, surface area, vessel diameter, basement membrane collagen, and microvessel phenotype within the regenerated tissue was dependent on the presence of SCs and rapamycin. TRITC-dextran injection demonstrated enhanced perfusion into scaffold channels. Rapamycin also increased the number of descending regenerated axons, as assessed by Fast Blue retrograde axonal tracing. These results demonstrate that normalization of the neovasculature was associated with enhanced axonal regeneration and improved function after spinal cord transection.