gebhart/patrepeval huggingface dataset:
Automated high throughput synthesis of [68]Ga-DOTATOC using the NaCl based labeling method (2012)

A number of bacterial lipases can be immobilized in a rapid and strong fashion on octyl-agarose gels (e.g., lipases from Candida antarctica, Pseudomonas fluorescens, Rhizomucor miehei, Humicola lanuginosa, Mucor javanicus, and Rhizopus niveus). Adsorption rates in absence of ammonium sulfate are higher than in its presence, opposite to the observation for typical hydrophobic adsorption of proteins. At 10 mM phosphate, adsorption of lipases is fairly selective allowing enzyme purification associated with their reversible immobilization. Interestingly, these immobilized lipase molecules show a dramatic hyperactivation. For example, lipases from R. niveus, M. miehei, and H. lanuginosa were 6-, 7-, and 20-fold more active than the corresponding soluble enzymes when catalyzing the hydrolysis of a fully soluble substrate (0.4 mM p-nitrophenyl propionate). Even higher hyperactivations and interesting changes in stereospecificity were also observed for the hydrolysis of larger soluble chiral esters (e.g. (R,S)-2-hydroxy-4-phenylbutanoic ethyl ester). These results suggest that lipases recognize these "well-defined" hydrophobic supports as solid interfaces and they become adsorbed through the external areas of the large hydrophobic active centers of their "open and hyperactivated structure". This selective interfacial adsorption of lipases becomes a very promising immobilization method with general application for most lipases. Through this method, we are able to combine, via a single and easily performed adsorption step, the purification, the strong immobilization, and a dramatic hyperactivation of lipases acting in the absence of additional interfaces, (e.g., in aqueous medium with soluble substrate). Copyright 1998 John Wiley & Sons, Inc.
Development of an advanced cybersecure radio for small unmanned ground vehicles (2017)

The Space and Naval Warfare (SPAWAR) Systems Center (SSC) Pacific is developing a prototype cybersecure network radio for the Man-Transportable Robotic System (MTRS) MK2 Mod 0 explosive-ordnance-disposal (EOD) robot that will also be compatible with the Advanced EOD Robotic System (AEODRS) Increment-2 robot. Both programs of record are managed by the Naval Surface Warfare Center (NSWC) Indian Head EOD Technology Division (IHEODTD). This paper describes SSC Pacific’s solution, the MTRS/AEODRS Radio for Cybersecure Operation with Network Integration (MARCONI) system. MARCONI is a modular networking radio developed based on the Joint Communication Architecture for Unmanned Systems (JCAUS), which is being defined by the Joint Ground Robotics Enterprise (JGRE). The system architecture includes a Payload Host Module, which provides translations and interfaces to the other modules, a Communication Module based on frequency-shifted and amplified 802.11n wireless technology with ad hoc networking, and a Cybersecurity Module, which uses the latest technologies—including Tactical Key Management—to meet the unique requirements for unmanned systems.
Quasi-elastic hadronic scattering at high momentum transfer (1997)

We measured the high-momentum quasi-elastic 12C(p,2p) reaction (at θcm≃90°) for 6 and 7.5 GeV/c incident protons. The validity of the QE picture and the impulse approximation was verified up to large Fermi momenta were it might be questionable. Transverse and longitudinal Fermi momentum distributions of the target proton were measured and compared to independent particle models which do not reproduce the large momentum tails. We also observed that the transverse Fermi distribution gets wider for smaller longitudinal components, in contrast to expectations from a simple Fermi gas model.
Synthesis and Characterization of Photosensitive Phosphorus Based Polymers Containing α,β‐Unsaturated Ketones in the Main Chain (2005)

Abstract 1,3‐Bis(4‐hydroxyphenyl)propenone (BHPP) and 3‐(4‐hydroxy‐3‐methoxy phenyl)‐1‐(4‐hydroxyphenyl)propenone (HMPHPP) were used as monomers for preparing photosensitive phosphorus containing polyesters. The photosensitive monomers BHPP and HMPHPP were prepared respectively by refluxing 4‐hydroxybenzaldehyde and 3‐methoxy‐4‐hydroxybenzaldehyde with 4‐hydroxy acetophenone. The polyesters were synthesized by interfacial polycondensation of photosensitive diols with N‐phenylphosphoramidic dichloride using hexadecyltrimethyl ammonium bromide (HDTMAB) as phase‐transfer catalyst. Copolymers were also prepared by incorporating terephthaloyl chloride in the polymer backbone. The synthesized monomers and polymers were characterized by UV, FT‐IR and 1H, 13C and 31P‐NMR spectroscopic techniques. The resulting polymers had inherent viscosities in the range of 0.15–0.51 dL/g and showed good solubility in polar organic solvents. The thermal properties of the polymers were studied by thermogravimetric analysis and differential scanning calorimetry under nitrogen atmosphere. The TGA data revealed that the 10% weight loss occurs at 275–320°C and all the synthesized polymers showed high char residues. DSC studies indicate that these polymers possess Tg in the range of 48 to 64°C. The photosensitive property of the polymers in film and solution state was investigated by ultraviolet spectroscopy. The effect of incorporation of terephthaloyl unit on photocrosslinking and thermal properties of the polymers was also studied. Keywords: photosensitive polymersphosphorus containingphotocrosslinkingpolycondensationinterfacial polymerization Acknowledgements Authors gratefully acknowledge the Principal and Management, PSG College of Technology, Coimbatore for providing the necessary facilities and the Department of Science and Technology, India for financial assistance. Sincere thanks are also due to SIF, Indian Institute of Science, Bangalore for NMR spectral studies and Elgitread (India) Ltd, Coimbatore for TGA studies.
Airborne laser communication terminal for intelligence, surveillance and reconnaissance (2004)

Sensors which collect information for intelligence, surveillance and reconnaissance generate a substantial amount of data. High data rates are required to transmit the information in reasonable time intervals. This paper describes the objectives and progress on the development of laser communication terminals for aircraft, transmitting high speed data over distances greater than 100 kilometers. The terminals are being designed and built under the Air Force Research Laboratory’s EO Sensor Technology & Evaluation Research (ESTER) program. The free space laser communication system consists of three terminals developed around commercial fiber-optic standards. They are designed to be an open system, full duplex, protocol neutral, and operate at 2.5 gigabit per second, capable of expanding to 10 gigabit per second. The terminal’s data rate can be reduced if atmospheric effects cause a significant increase in bit error rate. The free space optics are mounted in a pointing & tracking gimbal which interfaces with an avionics unit that measures aircraft GPS information. Fine pointing and tracking is maintained by using a beacon signal which switches from wide to narrow divergence when signal lock is achieved. The terminals are designed to operate in air-to-ground and air-to-air scenarios. Subsystem laboratory tests are being completed and the terminals are being prototyped. The first set of tests will be conducted between ground sites and the first flight demonstration will involve interfacing with the multi-purpose common data link (MP-CDL) on an aircraft. We provide a description of our terminal design, show our estimated operational performance, describe the results of laboratory testing, and outline the future program activities.
Supplementary Video 3 from Three-Dimensional Breast Cancer Models Mimic Hallmarks of Size-Induced Tumor Progression (2023)

&lt;p&gt;E-cad staining of 600 μm T47D microtumors migrating out of the hydrogel microwells on day 6&lt;/p&gt;
Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis (2009)

The skin as a surface organ is uniquely accessible for whole embryo/foetal analyses of developmental changes, such as gene induction, protein expression, formation of epidermal-derived appendages such as hair follicles and formation of the protective barrier. Such analyses have emphasised the heterogeneous nature of skin development, perhaps not surprisingly because epidermal development is programmed by heterogeneous underlying mesenchyme. It is necessary to account for this heterogeneity by precisely matching body sites when correlating sequential events during development, for example, the activation of gene expression, or comparing wild-type with mutant/knockout animals. In this chapter protocols designed to assay whole-mount in situ hybridisation and whole-mount barrier formation are presented. Formation of the protective barrier is the endpoint of epidermal terminal differentiation and defects in this process are reflected in failure, acceleration, or delay in barrier formation. Hence, these latter assays are of particular value as a rapid initial assay for epidermal developmental defects in genetically modified mice.
Non resonant quasiphase-matching by Fresnel birefringence: Towards an ultrawide tunability (2004)

We present a new quasiphase matching scenario based on the Fresnel birefringence occurring at total internal reflection, Thanks to non resonant conditions on the semiconductor plate thickness, ultrawide tunability is made possible (8-13 /spl mu/m).
Evaluation of load balancing in HSUPA (2015)

The paper considers a problem of load balancing, i.e. allocation of single-carrier User Equipment (UE) entities over multiple available carriers in the Wideband Code Division Multiple Access (W-CDMA) High Speed Uplink Packet Access (HSUPA) system. Performance of different load balancing approaches is numerically evaluated by means of System Level Simulations (SLS) of W-CDMA HSUPA. The simulation results demonstrate a sufficient performance gain due to optimal carrier selection at the beginning of a packet call compared to random carrier selection at the beginning of a call. An additional minor gain is achievable for high traffic loads if optimal carrier reassignment is allowed also during the packet calls. The gains in both cases are explained by better equalization of physical resources assigned to different UEs and by suppression of the intra-cell interference at the most loaded carriers.
Photochemical and radiolytic cleavage of 10‐methylacridine dimer in solutions and cryogenic glasses (1993)

Abstract 10‐Methylacridine dimer [(AcrH) 2 ] dissociates into a pair of 10‐methylacridyl radicals (AcrH˙) on laser excitation (308 nm) in solvents of various polarities. Exposure of (AcrH) 2 in methylcyclohexane glass at 77 K to γ‐rays of 60 Co generates the radical cation (AcrH) . Thermal annealing of the matrix to 90 K results in dissociation of (AcrH) into AcrH˙ and 10‐methylacridinium cation (AcrH + ).
Modelling of molecular interactions and inclusion phenomena in substituted β‐cyclodextrin: From simple probes to proteins (1999)

Abstract The ability of β‐cyclodextrin (βCD) to form stable complexes with α‐interferon was investigated. By using simple molecular mechanics approach interaction energy profiles of simple probes passing the center of βCD ring cavity along the main molecular symmetry axis were evaluated first. A computational study of host‐guest inclusion complexes between βCD and L‐α‐aminoacids and some selected pentapeptides was also carried out and aimed at understanding the nature of the driving forces and mechanism, leading to their formation. Relative complexation energies for the complexes and the solvation Gibbs free energies for single L‐α‐aminoacids were calculated. Both the aminoacid residue inside the βCD cavity and neighbouring residues were found to contribute to the stabilization of βCD complexes with the side‐chain of aminoacids present on the surface of α‐interferon. The most appropriate number of host βCD molecules for the encapsulation in the first shell of one α‐interferon molecule resulted to be 25.
Simple Aqueous Solution Route to Luminescent Carbogenic Dots from Carbohydrates (2009)

A simple aqueous solution route has been developed for the preparation of luminescent carbogenic dots from carbohydrates. The optical properties of the carbogenic dots can be tuned by differing the starting materials or the duration of nitric acid treatment. The multicolor emission capability and potential nontoxic nature of these carbogenic dots should enable them to find a wide range of applications in life sciences research.
Study of Metal-Carbon Nanofiber Interfaces for On-chip Interconnect Applications (2005)

Journal Article Study of Metal-Carbon Nanofiber Interfaces for On-chip Interconnect Applications Get access Y Ominami, Y Ominami Santa Clara University Search for other works by this author on: Oxford Academic Google Scholar Q Ngo, Q Ngo Santa Clara University Search for other works by this author on: Oxford Academic Google Scholar H Yoong, H Yoong Santa Clara University Search for other works by this author on: Oxford Academic Google Scholar A J Austin, A J Austin Santa Clara University Search for other works by this author on: Oxford Academic Google Scholar A M Cassell, A M Cassell NASA,Ames Search for other works by this author on: Oxford Academic Google Scholar Q Ye, Q Ye NASA,Ames Search for other works by this author on: Oxford Academic Google Scholar J Li, J Li NASA,Ames Search for other works by this author on: Oxford Academic Google Scholar M Meyyappan, M Meyyappan NASA,Ames Search for other works by this author on: Oxford Academic Google Scholar C Y Yang C Y Yang Santa Clara University Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 11, Issue S02, 1 August 2005, Pages 1964–1965, https://doi.org/10.1017/S1431927605500461 Published: 01 August 2005
Precise Fabrication of Uniform Molecular Gaps for Active Nanoscale Devices (2020)

Molecules with versatile functionalities and well-defined structures, can serve as building blocks for extreme nanoscale devices. This requires their precise integration into functional heterojunctions, most commonly in the form of metal-molecule-metal architectures. Structural damage and nonuniformities caused by current fabrication techniques, however, limit their effective incorporation. Here, we present a hybrid fabrication approach enabling uniform molecular gaps. Template-stripped lithographically-patterned gold electrodes with sub-nanometer roughness are used as the bottom contacts upon which the molecular layer is formed through self-assembly. The top contacts are assembled using dielectrophoretic trapping of colloidal gold nanorods, resulting in uniform sub-5 nm junctions. In these electrically-active designs, we further explore the possibility of mechanical tunability. The presence of molecules may help control sub-nanometer mechanical modulation which is conventionally difficult to achieve due to instabilities caused by surface adhesive forces. Our approach is versatile, providing a platform to develop and study active molecular gaps towards functional nanodevices.
Data from PD-1 Blockade in Solid Tumors with Defects in Polymerase Epsilon (2023)

&lt;div&gt;Abstract&lt;p&gt;Missense mutations in the polymerase epsilon (&lt;i&gt;POLE&lt;/i&gt;) gene have been reported to generate proofreading defects resulting in an ultramutated genome and to sensitize tumors to checkpoint blockade immunotherapy. However, many &lt;i&gt;POLE&lt;/i&gt;-mutated tumors do not respond to such treatment. To better understand the link between &lt;i&gt;POLE&lt;/i&gt; mutation variants and response to immunotherapy, we prospectively assessed the efficacy of nivolumab in a multicenter clinical trial in patients bearing advanced mismatch repair–proficient &lt;i&gt;POLE&lt;/i&gt;-mutated solid tumors. We found that only tumors harboring selective &lt;i&gt;POLE&lt;/i&gt; pathogenic mutations in the DNA binding or catalytic site of the exonuclease domain presented high mutational burden with a specific single-base substitution signature, high T-cell infiltrates, and a high response rate to anti–PD-1 monotherapy. This study illustrates how specific DNA repair defects sensitize to immunotherapy. POLE proofreading deficiency represents a novel agnostic biomarker for response to PD-1 checkpoint blockade therapy.&lt;/p&gt;Significance:&lt;p&gt;POLE proofreading deficiency leads to high tumor mutational burden with high tumor-infiltrating lymphocytes and predicts anti–PD-1 efficacy in mismatch repair–proficient tumors. Conversely, tumors harboring &lt;i&gt;POLE&lt;/i&gt; mutations not affecting proofreading derived no benefit from PD-1 blockade. POLE proofreading deficiency is a new tissue-agnostic biomarker for cancer immunotherapy.&lt;/p&gt;&lt;p&gt;&lt;i&gt;See related video: &lt;a href="https://vimeo.com/720727355" target="_blank"&gt;https://vimeo.com/720727355&lt;/a&gt;&lt;/i&gt;&lt;/p&gt;&lt;p&gt;&lt;i&gt;&lt;a href="https://aacrjournals.org/cancerdiscovery/article/doi/10.1158/2159-8290.CD-12-6-ITI" target="_blank"&gt;This article is highlighted in the In This Issue feature, p. 1397&lt;/a&gt;&lt;/i&gt;&lt;/p&gt;&lt;/div&gt;
Reflectivity of buried slab waveguides (2004)

The scattering properties of an abruptly ended buried slab waveguide for both TE and TM modes are examined by an improved iteration technique that is based on the integral equation method with "accelerating" parameters. The waveguide is considered a symmetrical slab, for which the weakly guiding conditions are invalid, and it is embedded in a different dielectric material. The tangential electric field distribution on the terminal plane, the reflection coefficient of the first TE and TM guided modes, and the far-field radiation pattern are computed. Numerical results are presented for several ended waveguides, while special attention is given to the far-field radiation pattern rotation and the terminal field distributions.
Nazumazoles D–F, Cyclic Pentapeptides That Inhibit Chymotrypsin, from the Marine Sponge Theonella swinhoei (2016)

Nazumazoles D-F (1-3) were isolated from the marine sponge Theonella swinhoei. The compounds gave extremely broad peaks by reversed-phase HPLC using an ODS column. HPLC using a gel permeation column was instrumental for the separation of the three compounds. Their planar structures were determined by interpretation of NMR data to be cyclic pentapeptides. Nazumazoles D-F contained one residue each of α-keto-l-norvaline (l-Knv) {or α-keto-d-leucine (l-Kle)}, l-alanyloxazole (l-Aox), d-Abu (or d-Ser), N-α-CHO-β-l-Dpr, and cis-4-methyl-l-proline. The absolute configuration of each amino acid residue was determined by Marfey's method in combination with conversion of the α-keto-β-amino acid to the α-amino acid by oxidation. Nazumazoles D-F are not cytotoxic against P388 cells at 50 μM, but inhibit chymotrypsin.
Flagellar dynamics of a connected chain of active, Brownian particles (2012)

Eukaryotic flagella are active structures with a complex architecture of microtubules, motor proteins and elastic links. They are capable of whiplike motions driven by motors sliding along filaments that are themselves constrained at an end. Here, we show that active, self-propelled particles that are connected together to form a single chain that is anchored at one end can produce the graceful beating motions of flagella. We use a combination of numerical simulations, scaling analysis and mean field continuum elastic theory to demarcate the phase diagram for this type of oscillatory motion as a function of the filament length, passive elasticity, propulsion force and longitudinal persistence of propulsion directions. Depending on the nature of the anchoring, we show that filament either undergoes flagella-like beating or assumes a steadily rotating coiled conformation. Our system is simpler than its biological inspiration, and thus could be experimentally realized using a variety of self-propelled particles.
Coordination−Insertion Copolymerization of Allyl Monomers with Ethylene (2011)

Coordination-insertion copolymerization of allyl monomers with ethylene was developed by using a palladium/phosphine-sulfonate catalyst. A variety of allyl monomers, including allyl acetate, allyl alcohol, protected allylamines, and allyl halides, were copolymerized with ethylene to form highly linear copolymers that possess in-chain -CH(2)CH(CH(2)FG)- units.
Targeted Molecular Dynamics Simulations of Protein Unfolding (2000)

The usefulness of targeted molecular dynamics (TMD) for the simulation of large conformational transitions is assessed in this work on the unfolding process of chymotrypsin inhibitor 2 (CI2). In TMD the force field is supplemented with a harmonic restraint which promotes either the increase of the conformational distance from the native state or the decrease of the distance from a target unfolded structure. As a basis of comparison, unfolding is also simulated by conventional, i.e., unrestrained, molecular dynamics at 375 and 475 K. In all simulations, an implicit approximation of solvation is used to adiabatically model the solvent response, which is appropriate for the nanosecond unfolding simulation method used here. In total, 44 TMD and 25 unrestrained high-temperature molecular dynamics simulations of CI2 unfolding were performed with an implicit solvation model that allowed more than 150 ns to be sampled. Qualitative agreement is found between the results of the TMD and unrestrained molecular dynamics at high temperature. The energies of the conformations sampled during TMD unfolding at 300 and 475 K are comparable to the ones obtained by conventional molecular dynamics at 375 and 475 K, respectively. The sequence of events, i.e., secondary and tertiary structure disruption, is similar in all unfolding simulations, despite the diversity of the pathways. Previous simulations of CI2 performed with different force fields and solvation models showed a similar sequence of events. This indicates that the TMD pathways are realistic even for very large conformational transitions such as protein unfolding.
Direct Casted LLZO-LITFSI Composite Solid-State Electrolyte for Lithium Ion Batteries (2021)

All-solid-state batteries (ASSBs) based on solid state electrolytes (SSE) has a promising future for safer lithium ion batteries (LIB) with high energy density. However, it has been challenging in large-scale fabricating an SSE separator with transport properties and comparable thickness to a traditional polypropylene separator used in lithium-ion batteries. This problem is especially exaggerated with oxide-based solid electrolytes, such as Li 7-3x Al x La 3 Zr 2 O 12 (LLZO), where thin films with micrometer scale in thickness using scalable techniques have not been realized. LLZO SSE separator is commonly made by fabricating pellet and followed by sintering. The resulting film must be thick enough to provide reasonable mechanical strength. However, the thick SSE separator will cause high resistance and low energy density. Here we present a direct casting technique to coat ultrathin LLZO-LITFSI composite electrolyte on the LiFePO 4 cathode. Through this technique, the electrolyte layers with different thickness ranging from 50 to 150 µm were prepared and studied electrochemically in ASSB using lithium as the anode. Without any further process and treatment, the ASSBs show a stable cycling performance with a reversible capacity &gt;150 mA h/g (~90% of the theoretical value). This work provides a potential scale-up strategy to manufacture stable and ultrathin garnet-based SSE for all-solid-state batteries. Acknowledgement We gratefully acknowledge the support from the U.S. Department of Energy's Vehicle Technologies Office. This work is conducted under the Cell Analysis, Modeling, and Prototyping (CAMP) Facility at Argonne National Laboratory. Argonne National Laboratory is operated for DOE office of Science by UChicago Argonne, LLC, under contract number DE-AC02-06CH11357.