gebhart/patrepeval huggingface dataset:
A Biomechanical Advantage of the Lengthening with an External Fixator Over an Intramedullary Nail: An Experimental Study in Saw Bones and Cadeveric Bones (2005)

Purpose: To know biomechanical differences in methods of limb lengthening between using monolateral external fixator and using external fixator over an intramedullary nail. Materials and Methods: In acryl rods, saw-bone, and cadeveric bone, we created two lengthening models of using monolateral external fixator and using external fixator over an intramedullary nail. The axial compression test was done on the site of osteotomy for lengthening. To fix the models, half pins of 5 mm in diameter and nails of 9 mm in diameter were used. Using MTS (Material Test System) machine, we evaluated the differences of axial stiffness according to the presence of an intramedullary nail or the numbers of half-pins which were fixed at each side of osteotomy. Results: Lengthening over an intramedullary nail, comparing to monolateral external fixator only, increased the axial stiffness by 1.1~1.2 times in acryl rods, 1.2~1.6 times in saw bones, and 15.6~15.9 times in cadeveric bones when the same numbers of half-pins were used. In saw bone and cadaveric bone, the group of two half pins in lengthening over an intramedullary nail was stiffer than the group of three pins in lengthening with monolateral external fixator. Conclusion: In the distraction of the limb, the addition of an intramedullary nail may increase the axial stiffness of the frame of monolateral external fixator. In lengthening over an intramedullary nail, it is enough to distract the bone with fixing two half pins at each sides of osteotomy.
Treatment with natural IFN of hepatitis C patients with or without antibodies to recombinant IFN. (1995)

The purpose of this study was to evaluate if a retreatment with natural human interferon alpha could lead to a recovery of the therapeutic responsiveness in non-responder patients with chronic hepatitis C who did (9 cases) or did not (14 cases) develop anti-interferon neutralizing antibodies while on treatment with recombinant interferon alpha2a. During retreatment, no patient developed detectable levels of neutralizing antibodies to natural interferon. At the end of retreatment, 6/9 positive patients showed a complete response to natural interferon therapy, while only 1/14 negative patients had a partial response. These data suggest that a second course of treatment with the natural preparation may be useful in patients who failed to respond to an earlier course of recombinant interferon, particularly the anti-interferon positive patients who showed hepatitis reactivation after an initial response concomitantly with antibody appearance. Conversely, a second course of natural interferon therapy might be useless in cases in which the lack of response is not associated to antibody development.
The effect of rotator cuff tears on reaction forces at the glenohumeral joint (2002)

The rotator cuff muscles maintain glenohumeral stability by compressing the humeral head into the glenoid. Disruption of the rotator cuff compromises concavity compression and can directly affect the loads on the glenohumeral joint. The purpose of this study was to quantify the effect of rotator cuff tears on the magnitude and direction of glenohumeral joint reaction forces during active shoulder abduction in the scapular plane using nine cadaveric upper extremities. Motion of the full upper extremity was simulated using a dynamic shoulder testing apparatus. Glenohumeral joint reaction forces were measured by a universal force-moment sensor. Five conditions of rotator cuff tears were tested: Intact, Incomplete Supraspinatus Tear, Complete Supraspinatus Tear, Supraspinatus/Infraspinatus Tear, and Global Tear. Reaction forces at the glenohumeral joint were found to steadily increase throughout abduction and peaked at maximum abduction for all conditions tested. There were no significant differences in reaction force magnitude for the intact condition (337 +/- 88 N) or those involving an isolated incomplete tear (296 +/- 83 N) or complete tear (300 +/- 85 N) of the supraspinatus tendon. Extension of tears beyond the supraspinatus tendon into the anterior and posterior aspect of the rotator cuff led to a significant decrease in the magnitude of joint reaction force (126 +/- 31 N). Similarly, such tears resulted in a significant change in the direction of the reaction force at the glenohumeral joint. These results suggest that joint reaction forces are significantly affected by the integrity of the rotator cuff, specifically, by the transverse force couple formed by the anterior and posterior aspects of the cuff. The quantitative data obtained in this study on the effect of rotator cuff tears on magnitude and direction of the reaction force at the glenohumeral joint helps clarify the relationship between joint motion, joint compression and stability.
Open reading frame 3, which is adjacent to the mycocerosic acid synthase gene, is expressed as an acyl coenzyme A synthase in Mycobacterium bovis BCG (1997)

The aim of this study was to test for expression of a 900-bp open reading frame (ORF), ORF3, located at the 5' end of the mycocerosic acid synthase gene in Mycobacterium bovis BCG and to determine the nature of the ORF3 protein. ORF3 was expressed as a 61-kDa C-terminal fusion protein with glutathione S-transferase in Escherichia coli. Polyclonal rabbit antiserum, prepared against this fusion protein, cross-reacted with a 65-kDa protein in M. bovis BCG crude extracts. Since this protein was larger than that predicted from the nucleotide sequence (32 kDa), ORF3 was resequenced, revealing an ORF of 1,749 bp that encodes a 64.8-kDa protein containing 583 amino acids. Reverse transcription-PCR revealed that ORF3 is expressed in M. bovis BCG. The ORF3 product has a high degree of similarity to the acyladenylate family of enzymes. Immunoaffinity absorption chromatography was used to isolate the 65-kDa cross-reacting protein from M. bovis BCG. This purified protein catalyzed coenzyme A (CoA) ester synthesis of n-C10 to n-C18 fatty acids but not mycocerosic acids. ORF3 antibodies severely inhibited acyl-CoA synthase activities of the purified protein and extracts of M. bovis BCG, Mycobacterium smegmatis, and E. coli. They also showed immunological cross-reactivity with proteins in these extracts. Both the ORF3 protein and the acyl-CoA synthase activity were located in the cell cytosol or were loosely associated with the cell membrane. These results indicate that ORF3 encodes an acyl-CoA synthase-like protein.
Distortion matrix concept for deep imaging in optical coherence microscopy (2019)

33 pages, 8 figures
Utilization of acid hydrolysate of recovered bacterial cell as a novel organic nitrogen source for L-tryptophan fermentation (2019)

In this study, waste bacterial cell (WBC) was recovered and used as an alternative to yeast extract in L-tryptophan fermentation. The effects of sulfuric acid concentration and temperature on the hydrolysis of WBC were optimized and the amino acid content in the waste bacterial cell hydrolysate (WBCH) was increased. Plackett-Burman and Box-Behnken design analysis revealed the optimum composition of the WBCH-based fermentation medium to be 22.47 g/L WBCH, 2.26 g/L KH<sub>2</sub>PO<sub>4</sub>, and 1.25 mg/L vitamin H. L-tryptophan yield and productivity with WBCH as the nitrogen source were 52.3 g/L and 2.16 g/L/h, respectively, which were 13% and 18% higher than those obtained with the yeast extract as the nitrogen source. In addition, WBCH did not affect the growth of Escherichia coli during L-tryptophan fermentation. Cost accounting showed that WBCH could be used as a novel and cheap organic nitrogen source for industrial L-tryptophan production.
ON THE GENERATION OF P AND S WAVE ENERGY IN CRYSTALLINE ROCKS* (1982)

A bstract A seismic source consisting of a 700 kg weight that could be dropped vertically or projected down a ramp inclined at 45° to the vertical was tested as a source of P, SV and SH waves within a crystalline rock body at Chalk River, Ontario. The seismic energy was recorded by arrays of both horizontal and vertical‐component geophones at distances between 30 and 600 m from the source, which was operated over glacial overburden varying in thickness from less than a meter to a few tens of meters. Seismic energy was more efficiently generated when the overburden thickness was at least several meters. The signals identified visually as S are generally true S, though some may be the converted wave PS. The SV amplitudes are generally larger than those of P, regardless of the type of shot, while the signal frequencies are roughly 60 Hz and 90 Hz, respectively. The horizontal‐component seismograms for the inclined shots showed no evidence of SH polarization, and the SH amplitudes were only rarely enhanced relative to P and SV amplitudes on changing from vertical to inclined shots. These unexpected results are attributed to the combined effect of the high velocity and density contrasts and the irregularity of the boundary between the glacial overburden and the rock body.
A Case Study of Passive Piles Failure in Open Excavation (2009)

This paper presents a case study in West Malaysia for passive piles failure in very soft marine clay in open excavation. In this particular case, the thickness of the very soft marine clay ranges from 5 m to 7 m (16 ft to 23 ft) from the ground surface. Preliminary site investigation reported SPT ‘N’ value of zero for the very soft marine clay. The design was based on a deep foundation utilizing driven piles to support the superstructure. During pile cap construction, the piles were subjected to uncontrolled open excavation, inducing bending moment in the piles, resulting in some cracked and broken piles. Analyses were carried out using an existing three-dimensional finite element code with a non-linear soil model to predict the response of these piles during excavation. Finally, the predictions of pile responses during excavation were compared satisfactorily with the observed results.
Pesticide (hexachlorocyclohexane) inhibition of growth and nitrogen fixation in blue‐ green algae Anabaenopsis raciborskii and Anabaena aphanizomenoides (1978)

Abstract The effects of the pesticide hexachlorocyclohexane (HCH) on the nitrogen fixing blue‐green algae Anabaenopsis raciborskii and Anabaena aphanizomenoides commonly found as blooms in fish ponds were studied. These algae were very sensitive to HCH, and a distinct decrease in growth rate was observed on prolonged incubation. Lower concentrations (10 μg/ml) were algistatic and higher concentrations (60 μg/ml) were algicidal. The inhibition of nitrogen fixation indicated that the presence of HCH might affect overall nitrogen economy of inland waters.
Cyclofunctionalization of Unsaturated Alcohols, Phenols, Acids, and Sulfonamides with 1‐Benzenesulfinyl Piperidine and Trifluoromethanesulfonic Anhydride. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Hypoglycemic Action of Fenfluramine in Diabetes Mellitus (1973)

Fenfluramine has been demonstrated to have a biphasic effect on glucose removal by skeletal muscle during forearm perfusion. A prompt early increase in glucose removal by skeletal muscle, lasting 90 to 120 minutes, disappeared with the release of free fatty acids. The metabolic effect of fenfluramine on glucose removal occurred without increase in lactate release, suggesting complete oxidation of glucose by skeletal muscle. The effect of fenfluramine on blood glucose was assessed in maturity onset and insulin-requiring diabetics. The drug was most suitable as a hypoglycemic agent when administered immediately before a meal in order that the maximum hypoglycemic action occurred during the period of post-absorption hyperglycemia. When administered before a meal fenfluramine consistently lowered blood glucose levels in maturity onset diabetes mellitus for at least two hours. A similar but less marked action was seen in insulin-requiring diabetics. Blood glucose levels were lowered by fenfluramine in diabetics who were maintained on diet alone or diet plus tolbutamide without the risk of lactic acidosis and without any direct effect on insulin secretion.
SMAD, ERK1/2 AND PI3 KINASE PATHWAYS REGULATE TRANSFORMING GROWTH FACTOR-β-INDUCED AGGRECAN GENE EXPRESSION IN NUCLEUS PULPOSUS CELLS (2005)

To investigate which TGF-β played a crucial roles, we examined expression levels of the TGF-β1, -β2 and -β3 in the human intervertebral discs by real-time PCR. NP cell preparation and analysis of TGF-β−mediated signaling pathways. Lumbar intervertebral discs from four consecutive levels (L2-3, L3-4, L4-5 and L5-S1) were dissected from the spines of adolescent Japanese white rabbits (1-1.5 kg, 6-9 weeks old) after sacrificing by injection of an excess amount of sodium pentobarbital. Tissue was harvested from the NP, and cells were isolated using a sequential proteinase-collagenase digestion. NP cells in monolayer culture were then stimulated with 1 ng/ml TGF-β and treated with cycloheximide. The cells were also treated with SB-431542 (TGF-β type I receptor kinase inhibitor), LY294002 (phosphatidylinositol-3 kinase inhibitor), or U0126 (MEK1/2 inhibitor) at the indicated concentration and the changes in aggrecan gene expression were analyzed by real-time PC Transient transfection and siRNA for R-Smads NP cells were suspended in sodium alginate (1.2% solution in 155 mM NaCl) and maintained for up to 14 days with Chondrocyte Differentiation Media. Cells were then recovered and seeded in a 12-well plate at a density of 1 x 10 5
Cell Autonomous Phosphoinositide 3-Kinase Activation in Oocytes Disrupts Normal Ovarian Function Through Promoting Survival and Overgrowth of Ovarian Follicles (2015)

In this study, we explored the effects of oocytic phosphoinositide 3-kinase (PI3K) activation on folliculogensis by generating transgenic mice, in which the oocyte-specific Cre-recombinase induces the expression of constitutively active mutant PI3K during the formation of primordial follicles. The ovaries of neonatal transgenic (Cre+) mice showed significantly reduced apoptosis in follicles, which resulted in an excess number of follicles per ovary. Thus, the elevation of phosphatidylinositol (3,4,5)-trisphosphate levels within oocytes promotes the survival of follicles during neonatal development. Despite the increase in AKT phosphorylation, primordial follicles in neonatal Cre+ mice remained dormant demonstrating a nuclear accumulation of phosphatase and tensin homolog deleted on chromosome 10 (PTEN). These primordial follicles containing a high level of nuclear PTEN persisted in postpubertal females, suggesting that PTEN is the dominant factor in the maintenance of female reproductive lifespan through the regulation of primordial follicle recruitment. Although the oocytic PI3K activity and PTEN levels were elevated, the activation of primordial follicles and the subsequent accumulation of antral follicles with developmentally competent oocytes progressed normally in prepubertal Cre+ mice. However, mature Cre+ female mice were anovulatory. Because postnatal day 50 Cre+ mice released cumulus-oocyte complexes with developmentally competent oocytes in response to super-ovulation treatment, the anovulatory phenotype was not due to follicular defects but rather endocrine abnormalities, which were likely caused by the excess number of overgrown follicles. Our current study has elucidated the critical role of oocytic PI3K activity in follicular function, as well as the presence of a PTEN-mediated mechanism in the prevention of immature follicle activation.
Dynamic Refinement Network for Oriented and Densely Packed Object Detection (2020)

Object detection has achieved remarkable progress in the past decade. However, the detection of oriented and densely packed objects remains challenging because of following inherent reasons: (1) receptive fields of neurons are all axis-aligned and of the same shape, whereas objects are usually of diverse shapes and align along various directions; (2) detection models are typically trained with generic knowledge and may not generalize well to handle specific objects at test time; (3) the limited dataset hinders the development on this task. To resolve the first two issues, we present a dynamic refinement network that consists of two novel components, i.e., a feature selection module (FSM) and a dynamic refinement head (DRH). Our FSM enables neurons to adjust receptive fields in accordance with the shapes and orientations of target objects, whereas the DRH empowers our model to refine the prediction dynamically in an object-aware manner. To address the limited availability of related benchmarks, we collect an extensive and fully annotated dataset, namely, SKU110K-R, which is relabeled with oriented bounding boxes based on SKU110K. We perform quantitative evaluations on several publicly available benchmarks including DOTA, HRSC2016, SKU110K, and our own SKU110K-R dataset. Experimental results show that our method achieves consistent and substantial gains compared with baseline approaches. The code and dataset are available at https://github.com/Anymake/DRN_CVPR2020.
HACS: Helical Auxetic Yarn Capacitive Strain Sensors with Sensitivity Beyond the Theoretical Limit (Adv. Mater. 10/2023) (2023)

Capacitive Strain Sensors In article number 2209321, Tyler J. Cuthbert, Carlo Menon, and co-workers present a novel capacitive strain sensor that accesses sensitivity beyond the theoretical limit. The helical auxetic capacitive sensor (HACS) employs a two-component yarn structure allowing tuneable sensitivity and accessible strain ranges. The HACS sensitivity is found to be from an engulfment phenomenon, which is predicted with a geometric model and analyzed by X-ray μCT scanning.
Equality of streams is a Π0 over 2-complete problem (2006)

This paper gives a precise characterization for the complexity of the problem of proving equal two streams defined with a finite number of equations: Π0 over 2. Since the Π 0 over 2 class includes properly both the reursively enumerable and the corecursively enumerable classes, this result implies that neither the set of pairs of equal streams nor the set of pairs of unequal streams is recursively enumerable. Consequently, one can find no algorithm for determining equality of streams, as well as no algorithm for determining inequality of streams. In particular, there is no complete proof system for equality of streams and no complete system for inequality of streams.
Induction of WNT inhibitory factor 1 expression by Müllerian inhibiting substance/antiMullerian hormone in the Müllerian duct mesenchyme is linked to Müllerian duct regression. (2014)

A key event during mammalian sexual development is regression of the Mullerian ducts (MDs) in the bipotential urogenital ridges (UGRs) of fetal males, which is caused by the expression of Mullerian inhibiting substance (MIS) in the Sertoli cells of the differentiating testes. The paracrine signaling mechanisms involved in MD regression are not completely understood, particularly since the receptor for MIS, MISR2, is expressed in the mesenchyme surrounding the MD, but regression occurs in both the epithelium and mesenchyme. Microarray analysis comparing MIS signaling competent and Misr2 knockout embryonic UGRs was performed to identify secreted factors that might be important for MIS-mediated regression of the MD. A seven-fold increase in the expression of Wif1, an inhibitor of WNT/β-catenin signaling, was observed in the Misr2-expressing UGRs. Whole mount in situ hybridization of Wif1 revealed a spatial and temporal pattern of expression consistent with Misr2 during the window of MD regression in the mesenchyme surrounding the MD epithelium that was absent in both female UGRs and UGRs knocked out for Misr2. Knockdown of Wif1 expression in male UGRs by Wif1-specific siRNAs beginning on embryonic day 13.5 resulted in MD retention in an organ culture assay, and exposure of female UGRs to added recombinant human MIS induced Wif1 expression in the MD mesenchyme. Knockdown of Wif1 led to increased expression of β-catenin and its downstream targets TCF1/LEF1 in the MD mesenchyme and to decreased apoptosis, resulting in partial to complete retention of the MD. These results strongly suggest that WIF1 secretion by the MD mesenchyme plays a role in MD regression in fetal males.
Hirudisins. Hirudin-derived thrombin inhibitors with disintegrin activity. (1992)

Recombinant hirudin variants have been designed which inhibit alpha-thrombin by the hirudin mechanism and which in addition exhibit disintegrin activity. These proteins, called "hirudisins," have been engineered by replacing the Ser-Asp-Gly-Glu sequence at the tip of hirudin's finger-like structure (residues 32-35) by Arg-Gly-Asp-Ser (RGDS) to yield hirudisin and Lys-Gly-Asp-Ser (KGDS) to obtain hirudisin-1. Comparison of thrombin inhibition activities showed that hirudisin is 2-fold more potent (K(i) = 160 +/- 70 fM) than hirudisin-1 (K(i) = 370 +/- 44 fM) and recombinant (r)-hirudin (K(i) = 270 +/- 50 fM). alpha-Thrombin-stimulated platelet aggregation was effectively inhibited by r-hirudin, hirudisin, and hirudisin-1 with IC50 of 5.7 to 6.8 nM. Unlike r-hirudin, hirudisin inhibits ADP-induced platelet aggregation (IC50 = 65 microM) 3- to 5-fold stronger than the linear GRGDS- and RGDS-peptide. Direct interaction of hirudisin with purified glycoprotein IIb-IIIa demonstrated that antiplatelet aggregation activity is due to the integrin-directed RGD motif. Disintegrin activity of hirudisin relative to that of reduced and carboxymethylated hirudisin suggests that the conformational strain favors binding to integrins. On the basis of these results, hirudisins appear to be interesting molecules for the design of potential antithrombotic agents with antithrombin as well as antiplatelet aggregation activities.
The Effects of Culture Conditions on the Secretion of Human Lysozyme bySaccharomyces cerevisiaeA2-1-1A (1989)

The effects of initial glucose concentrations on the cell growth, glucose usage, and human lysozyme (HLY) secretion under the ENOl promoter were examined in the Saccharomyces cerevisiae strain A2–1–1A harboring a multicopy plasmid. By increasing the initial glucose from 2 % to 10 %, the HLY secretion increased 7 ~ 8 fold although the cell growth was not affected. By adding a mixture of mineral salts to the basal medium, the HLY secretion was increased about twice due to the continuity of the HLY expression at the stationary phase of cell growth. The high HLY secretion (5.5 mg per liter, 47-fold higher than the original level) was achieved by the strain A2–1–1A grown in the synthetic basal medium containing 10% initial glucose, and supplemented with mineral salts containing ammonium sulfate, potassium phosphate, potassium chloride, magnesium sulfate, and iron sulfate.