gebhart/patrepeval huggingface dataset:
Dynamic changes of interleukin 2 (IL-2) and circulating tumor cells (CTCs) in patients with advanced breast cancer (BCa) after systemic therapies. (2018)

1090 Background: CTCs play a critical role in BCa metastasis and worse prognosis even after systemic treatments such as chemotherapy and biological therapy. The mechanisms that contribute to CTCs migration and tumor aggressiveness remain unknown. Herein, we report a novel finding of the correlation between plasma IL-2 and the changes of CTC for BCa patients after therapies. Methods: Duplicate whole blood samples (7.5ml/each) were collected in EDTA tubes from 43 patients with stage III/IV BCa patients before (baseline, BL) or 3 months after (first evaluation, FE) systemic therapies respectively from patients treated at NMH. CTC enrichment and enumeration were performed in FDA approved CELLTRACKS ANALYZERII System and were linked with clinical database. The CTCs were classified as CK+, EpCAM+, DAPI+ and CD25-. ELISA for IL-2 was performed by using patients’ plasma. Kruskal-Wallis test was used for statistics. Results: CTCs were detected in 23 patients at BL and 21 patients at FE of blood draws respectively. CTCs count increased in 12 patients (Group 1: from 102.9 to 213.3), decreased in 14 patients (Group 2: from 23 to 13.6) and remained unchanged as 0 in 17 patients (Group 3) respectively when BL was compared to the FE. The corresponding changes of IL-2 were -17.4pg/ml, 8.04pg/ml and -4.59pg/ml in Group 1, 2 and 3 respectively. The BL level of IL-2 in Group 1 is significantly higher than the other two groups (p = 0.027), and decreased of CTCs is associated with increased IL-2 (P < 0.01), while decreased IL-2 in FE level is associated with increased of CTCs after therapy in whole population (p < 0.001). More important, IL-2 dropped significantly in patients with CTC stably ≥5 or who experienced a rise in CTC ≥5 at the FE compared to the BL time point (p < 0.001). Conclusions: The study demonstrated for the first time a reverse correlation between IL-2 level and CTCs count. The data suggest that the more aggressive BCa (CTC≥5) induce the immune response (high levels of IL2 at baseline), but subsequently the IL2 level will decrease in patients that fail to benefit from treatment. We propose that surveillance of immune cytokine will help to understand advanced BCa prognosis and predict treatment benefit.
Burnett, Frances Eliza Hodgson, [1896?] [June?] [17?] (1895)

Envelope and transcription included; only year in letter but postmark on envelope gives June 3, 1895; PDF Copy (MS note)
Th17 immune response to adipose tissue‐derived mesenchymal stromal cells (2019)

Adipose tissue-derived mesenchymal stromal cells (ASCs) hold the promise of achieving successful immunotherapeutic results due to their ability to regulate different T-cell fate. ASCs also show significant adaptability to environmental stresses by modulating their immunologic profile. Cell-based therapy for inflammatory diseases requires a detailed understanding of the molecular relation between ASCs and Th17 lymphocytes taking into account the influence of inflammation and cell ratio on such interaction. Accordingly, a dose-dependent increase in Th17 generation was only observed in high MSC:T-cell ratio with no significant impact of inflammatory priming. IL-23 receptor (IL-23R) expression by T cells was not modulated by ASCs when compared to levels in activated T cells, while ROR-γt expression was significantly increased reaching a maximum in high (1:5) unprimed ASC:T-cell ratio. Finally, multiplex immunoassay showed substantial changes in the secretory profile of 15 cytokines involved in the Th17 immune response (IL-1β, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-22, IL-21, IL-23, IL-25, IL-31, IL-33, IFN-γ, sCD40, and TNF-α), which was modulated by both cell ratio and inflammatory priming. These findings suggest that Th17 lymphocyte pathway is significantly modulated by ASCs that may lead to immunological changes. Therefore, future ASC-based immunotherapy should take into account the complex and detailed molecular interactions that depend on several factors including inflammatory priming and cell ratio.
Measurement of nuclear inelastic scattering cross section of thorium-232 for 144 keV Si-filtered neurons. (1983)

The nuclear inelastic scattering cross section of 232Th has been measured at 144 keV using a Si-filtered neutron beam from the University of Missouri Research Reactor (MURR). The energy spectrum of the scattered neutrons was measured with a spherical hydrogen-gas counter and a pulse-height-unfolding procedure. Data were taken at nine scattering angles from 30° to 150° in 15° increments. The results provide new experimental information below 250 keV. The angle-integrated cross section is 0.74±0.05 barn, which agrees with the evaluation JENDL-2 and with a coupled-channel calculation where the inelastic scattering through the direct-excitation process of the collective rotational motion of a deformed nucleus is included as well as that through the compound-nucleus-formation process. Experimental results have been obtained also for the angular dependence of the elastically scattered neutrons at 144 keV.
Preparation and Characterization of Organotin–Oxomolybdate Coordination Polymers and Their Use in Sulfoxidation Catalysis (2003)

The organotin-oxomolybdates [(R(3)Sn)(2)MoO(4)].n H(2)O (R=methyl, n-butyl, cyclohexyl, phenyl, benzyl) have been prepared and tested as catalysts for the oxidation of benzothiophene with aqueous hydrogen peroxide, at 35 degrees C and atmospheric pressure. In all cases, the 1,1-dioxide was the only observed product. The kinetic profiles depend on the nature of the tin-bound R group and also on the addition of a co-solvent. For the tribenzyltin derivative, the apparent activation energies for sulfoxidation as a function of the co-solvent are in the order 1,2-dichloroethane (5 kcal mol(-1))<ethanol<acetonitrile<water (27 kcal mol(-1)). The solid could be recovered by centrifugation from the three-phase (solid-liquid-liquid) system containing 1,2-dichloroethane. The catalyst was reused in a second reaction cycle with no significant loss of activity. Increasing the oxidant/substrate ratio from 2:1 to 6:1 allows the corresponding sulfone to be obtained in quantitative yield within 24 h. Changing the nature of the tin-bound R group in the catalyst precursors modifies their physical properties and hence their catalytic performance. The variation in hydrophobic/hydrophilic character is important, since the Me, nBu and Cy derivatives crystallize as anhydrous compounds, whereas the other two derivatives are hydrates. The polymers also have different structures, as evidenced by Xray powder diffraction. Mo K-edge and Sn K-edge EXAFS spectroscopy confirmed that the structures arise from the self-assembly of tetrahedral [MoO(4)](2-) subunits and [R(3)Sn](+) spacers. The Mo...Sn separation in the trimethyltin derivative is a uniform 3.84 A. By contrast, the EXAFS results revealed the coexistence of short (3.67-3.79 A) and long (3.93-4.07 A) Mo.Sn separations in the other coordination polymers. The catalyst precursors were also characterized in the solid state by thermogravimetric analysis, FTIR, and Raman spectroscopy, and MAS NMR ((13)C, (119)Sn) spectroscopy.
Baro-Activated Neurons With Pulse-Modulated Activity in the Rat Caudal Ventrolateral Medulla Express GAD67 mRNA (2003)

GABAergic neurons in the caudal ventrolateral medulla (CVLM) are believed to mediate the sympathetic baroreceptor reflex by inhibiting presympathetic neurons in the rostral ventrolateral medulla (RVLM). Accordingly, some CVLM neurons are activated by increased arterial pressure (AP; baro-activated), have activity strongly modulated by the AP pulse (pulse-modulated), and can be antidromically activated from the RVLM. This study examined whether baro-activated, pulse-modulated CVLM neurons are indeed GABAergic and examined their structures. We recorded extracellularly from 19 baro-activated, pulse-modulated CVLM neurons in chloralose-anesthetized rats. Most of these cells (13/19) were silenced by decreasing AP with nitroprusside, but some (6/19) remained active at low AP levels. They were also excited by phenyl biguanide (17/17) but inhibited by noxious tail pinch (8/11). Twelve baro-activated cells were filled with biotinamide and examined for expression of GAD67 mRNA. Because adjacent vagal motor neurons are also activated by increased AP, we examined choline acetyltransferase (ChAT) immunoreactivity. Most baro-activated cells (9/12) expressed high levels of GAD67 mRNA, the rest (3/12) displayed lower levels of GAD67 mRNA, but none showed ChAT immunoreactivity. In contrast, adjacent baro-inhibited CVLM cells had no GAD67 mRNA (n = 5) but were instead tyrosine hydroxylase immunoreactive (n = 7). Reconstruction of baro-activated CVLM neurons revealed axons that projected dorsomedially and rostrally with several axon collaterals. These data demonstrate the existence of GABAergic CVLM neurons with the physiological characteristics expected of interneurons that mediate the sympathetic baroreceptor reflex. In addition, baro-activated GABAergic CVLM neurons appear to integrate several types of inputs and provide inhibition to multiple targets.
The poplar PtrWNDs are transcriptional activators of secondary cell wall biosynthesis (2010)

Secondary cell walls, consisting of cellulose, hemicelluloses and lignin, make up the bulk of wood biomass. It is therefore expected that dissection of the molecular mechanisms underlying secondary wall biosynthesis and its regulation will be instrumental to unravel the process of wood formation in tree species. Wood formation requires the coordinated activation of genes in the secondary wall biosynthetic program that is essential for the biosynthesis and assembly of wood components. It has recently been discovered that a group of poplar (Populus trichocarpa) wood-associated NAC domain transcription factors, PtrWNDs, which are functional orthologs of the Arabidopsis SND1, are capable of turning on the entire secondary wall biosynthetic program when expressed in Arabidopsis. In addition, two of the PtrWNDs were found to be able to activate the promoters of poplar wood biosynthetic genes and a number of other poplar wood-associated transcription factors. Further testing reveals that the promoters of these poplar wood-associated transcription factors are also activated by other PtrWNDs. It is therefore proposed that PtrWNDs are master transcriptional switches regulating a cascade of downstream transcription factors and thereby mediate the coordinated activation of wood biosynthetic genes during wood formation.
Adoption de deux articles additionnels au décret sur les municipalités, lors de la séance du 28 décembre 1789 (1880)

Target Guy Jean-Baptiste, Démeunier Jean Nicolas, Garat Aîné Dominique, Bouche Charles-François, Montlosier François Dominique de Reynaud, comte de, Regnaud de Saint-Jean d'Angely Michel Louis Etienne, Mougins de Roquefort Jean Joseph, Robespierre Maximilien François Marie Isidore Joseph de, Kytspotter Jean-Baptiste Louis de. Adoption de deux articles additionnels au décret sur les municipalités, lors de la séance du 28 décembre 1789. In: Tome XI - Du 24 décembre 1789 au 1er mars 1790. p. 32.
Synthesis and characterization of core/shell ionomeric latexes. I. Latex and film properties (1995)

Abstract This research work involved studies on the synthesis and characterization of core/shell ionomeric latexes in order to understand the process of ionic aggregate formation. The core/shell structure of the ionomeric latexes prepared in this study confines the ionic domains to the copolymeric matrix within the shell polymer, and in addition, benefits from the ionomeric properties and core/shell structure simultaneously. Core/shell ionomeric latexes with polystyrene cores and styrene/ n ‐butyl acrylate/methacrylic acid terpolymer shells were synthesized by a semicontinuous seeded emulsion copolymerization process. The distribution of the methacrylic acid in the core/shell latexes was determined using conductometric titration techniques. The results of the conductometric titration analysis indicate that the principal locus of the carboxyl groups in the core/shell latexes is at the particle surface, but specific information about the differences in the distribution of methacrylic acid inside the latex particles could not be obtained. Mechanical characterization of the core/shell ionomeric latex films was carried out to understand the structure‐property relationships using Dynamic Mechanical Analysis (DMA) and tensile testing. It was discovered that the core/shell latexes exhibited significantly different properties, owing to the differences in the overall carboxyl content and surface loading. Extensive studies were carried out to study the effect of water plasticization on the mechanical behavior of the core/shell ionomeric latex films; water adsorption causes the solvation of ionic aggregates. A preliminary investigation of the effect of the type of counterion used for the neutralization of carboxyl groups on the properties of core/shell ionomeric latex films was also carried out. © 1995 John Wiley &amp; Sons, Inc.
The efficacy of CRISPR-Cas9-mediated induction of the CCR5delta32 mutation in the human embryo (2018)

The editing of the CCR5 gene in the CD4+ T cell genome is an effective way of preventing HIV-1 proliferation. Very similar strategies can be used to protect the fetus of an HIV-infected female showing a weak response to antiretroviral therapy. Inducing the “natural” CCR5delta32 mutation in a zygote may guard the fetus against HIV infection both in utero and at birth. In this study, we optimize the CRISPR-Cas9 system to induce a homozygous 32-nt deletion similar to the naturally occurring CCR5delta32 allele in the human zygote at the S-phase. Edits were done in the abnormal tripronuclear zygotes unsuitable for IVF. Sixteen tripronuclear zygotes in the S-phase obtained from WT CCR5 donors were injected with an original CRISPR-Cas9 system designed by the authors. Upon injection, the zygotes were transferred into the Blastocyst (COOK) embryo culture medium and cultured for 5 days in a CO2 incubator until blastocysts were formed (approximately 250 cells). Eight zygotes that successfully developed into blastocysts were PCR-genotyped to analyze the efficacy of genome editing. Of 16 zygotes injected with CRISPR-Cas9, only 8 reached the blastocyst stage. PCR genotyping revealed the absence of the initial WT CCR5 variant in 5 of 8 blastocysts (100% CCR5delta32 homozygous). Two had about 3% and one about 20% of WT CCR5 mosaicism. This leads us to conclude that the efficacy of the proposed CRISPR-Cas9 system for the induction of the CCR5delta32 mutation in human embryos is very high producing more than 50% of completely modified embryos.
Pictures of Combinatorial Cubes (2017)

We prove that the 8-point algorithm always fails to reconstruct a unique fundamental matrix $F$ independent on the camera positions, when its input are image point configurations that are perspective projections of the vertices of a combinatorial cube in $\mathbb{R}^3$. We give an algorithm that improves the 7- and 8-point algorithm in such a pathological situation. Additionally we analyze the regions of focal point positions where a reconstruction of $F$ is possible at all, when the world points are the vertices of a combinatorial cube in $\mathbb{R}^3$.
The Preliminary Assessment of New Biomaterials Necessitates a Comparison of Direct and Indirect Cytotoxicity Methodological Approaches (2022)

Comparison of both methods shows that it is pivotal to investigate biomaterials at the very early stages using both indirect and direct methods to access the influence of the released toxins and surface of the material on the cell condition.
Medium Access Control (2008)

This chapter contains sections titled: Introduction Fundamental MAC Protocols MAC Design for Wireless Sensor Networks MAC Protocols for Wireless Sensor Networks Summary and Future Directions References
VISIT-TS video (release 20160516) (2016)

<strong>VISIT-TS</strong> (<strong>V</strong>ideo-<strong>I</strong>ntegrated <strong>S</strong>creening <strong>I</strong>nstrument for <strong>T</strong>ics and <strong>T</strong>ourette <strong>S</strong>yndrome) is a multimedia tool intended to demonstrate tics to a lay audience, to discuss their defining and common attributes, and to address features that differentiate tics from other movements and vocalizations. The video includes a teaching section (5 minutes) followed by questions (5 minutes). The original intended use of VISIT-TS is for epidemiological research. Vachon MJ, Striley CW, Gordon MR, Schroeder ML, Bihun EC, Koller JM, Black KJ: VISIT-TS: A multimedia tool for population studies on tic disorders [<em>version 2; referees: 3 approved</em>]. F1000Res 2016; 5:1518. DOI: 10.12688/f1000research.7196.2.
Glycosylation Mutants of Dengue Virus NS1 Protein (1994)

The non-structural glycoprotein NS1 of dengue virus type 2 contains sites for N-linked glycosylation at Asn-130 and Asn-207. NS1 synthesized in infected cells is glycosylated at both locations. We have now examined the dimerization and secretion of NS1 lacking one or both of these sites by transient expression of mutagenized cDNA inserted into a simian virus 40-based vector. Immunoblotting and radioimmunoprecipitation were used to detect NS1 associated with transfected cells and in the extracellular medium. Elimination of one or both glycosylation sites did not abolish dimerization and secretion of NS1. However, NS1 lacking Asn-207 showed reduced dimer stability and secretion. Treatment of secreted NS1 with endoglycosidase H demonstrated that complex glycans were attached at Asn-130 and high-mannose glycans at Asn-207.
Intermolecular interactions between σ- and π-holes of bromopentafluorobenzene and pyridine: computational and experimental investigations (2018)

The characters of σ- and π-holes of bromopentafluorobenzene (C6F5Br) enable it to interact with an electron-rich atom or group like pyridine which possesses an electron lone-pair N atom and a π ring. Theoretical studies of intermolecular interactions between C6F5Br and C5H5N have been carried out at the M06-2X/aug-cc-pVDZ level without and with the counterpoise method, together with single point calculations at M06-2X/TZVP, wB97-XD/aug-cc-pVDZ and CCSD(T)/aug-cc-pVDZ levels. The σ- and π-holes of C6F5Br exhibiting positive electrostatic potentials make these sites favorably interact with the N atom and the π ring of C5H5N with negative electrostatic potentials, leading to five different dimers connected by a σ-holen bond, a σ-holeπ bond or a π-holeπ bond. Their geometrical structures, characteristics, nature and spectroscopy behaviors were systematically investigated. EDA analyses reveal that the driving forces in these dimers are different. NCI, QTAIM and NBO analyses confirm the existence of intermolecular interactions formed via σ- and π-holes of C6F5Br and the N atom and the π ring of C5H5N. The experimental IR and Raman spectra gave us important information about the formation of molecular complexes between C6F5Br and C5H5N. We expect that the results could provide valuable insights into the investigation of intermolecular interactions involving σ- and π-holes.
Electronic properties of bis(.eta.5-cyclopentadienyl)titanium 2,2'-bipyridyl. A singlet molecule with a low-lying triplet excited state (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic properties of bis(.eta.5-cyclopentadienyl)titanium 2,2'-bipyridyl. A singlet molecule with a low-lying triplet excited stateArlene Mootz McPherson, Benjamin F. Fieselmann, Dennis L. Lichtenberger, Gary L. McPherson, and Galen D. StuckyCite this: J. Am. Chem. Soc. 1979, 101, 13, 3425–3430Publication Date (Print):June 1, 1979Publication History Published online1 May 2002Published inissue 1 June 1979https://pubs.acs.org/doi/10.1021/ja00507a001https://doi.org/10.1021/ja00507a001research-articleACS PublicationsRequest reuse permissionsArticle Views278Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The electron-cyclotron maser instability as a source of plasma radiation (1986)

The generation of continuum bursts from the sun at dm and m wavelengths (in particular, type IV bursts) via the electron-cyclotron-maser instability is examined. The maser instability can be driven by an electron distribution with either a loss-cone anisotropy or a peak at large pitch angles. For omega(p)/Omega(e) much greater than 1, the maser emission is produced by electrons interacting through a harmonic (cyclotron) resonance and is electrostatic, being in the upper hybrid mode at frequencies approximately equal to omega(p). Coalescence processes are required to convert the electrostatic waves into transverse radiation which can escape from the source region. Whether the resultant spectrum is nearly a smooth continuum or has a zebra-stripe pattern (both of which occur in type IV bursts) depends on the form of the electron distribution, inhomogeneities in the density and magnetic field, and whether the maser reaches saturation. For at least the case of some type IV dm bursts with fine structure, comparison with observations seems to indicate that the electrons producing the emission are more likely to have a loss-cone distribution, and that the maser instability is not at saturation.