gebhart/patrepeval huggingface dataset:
Small RNA-dependent Expression of Secondary Metabolism Is Controlled by Krebs Cycle Function in Pseudomonas fluorescens (2009)

Pseudomonas fluorescens CHA0, an antagonist of phytopathogenic fungi in the rhizosphere of crop plants, elaborates and excretes several secondary metabolites with antibiotic properties. Their synthesis depends on three small RNAs (RsmX, RsmY, and RsmZ), whose expression is positively controlled by the GacS-GacA two-component system at high cell population densities. To find regulatory links between primary and secondary metabolism in P. fluorescens and in the related species Pseudomonas aeruginosa, we searched for null mutations that affected central carbon metabolism as well as the expression of rsmY-gfp and rsmZ-gfp reporter constructs but without slowing down the growth rate in rich media. Mutation in the pycAB genes (for pyruvate carboxylase) led to down-regulation of rsmXYZ and secondary metabolism, whereas mutation in fumA (for a fumarase isoenzyme) resulted in up-regulation of the three small RNAs and secondary metabolism in the absence of detectable nutrient limitation. These effects required the GacS sensor kinase but not the accessory sensors RetS and LadS. An analysis of intracellular metabolites in P. fluorescens revealed a strong positive correlation between small RNA expression and the pools of 2-oxoglutarate, succinate, and fumarate. We conclude that Krebs cycle intermediates (already known to control GacA-dependent virulence factors in P. aeruginosa) exert a critical trigger function in secondary metabolism via the expression of GacA-dependent small RNAs.
Characterization of Novel Calorimeters in the Annular Core Research Reactor [presentation]. (2014)

Abstract not provided.
A Decentralized Naming Facility (1986)

A key component in distributed computer systems is the naming facility: the means by which highlevel names are bound to objects and by which objects are located given only their names. We describe the design, implementation, and performance of a decentralized naming facility, in which the global name space and name mapping mechanism are implemented by a set of cooperating peers, with no central authority. Decentralization is shown to lend increased extensibility and reliability to the design. Efficiency in name mapping is achieved through specialized caching techniques. Categories and Subject Descriptors: C.2.4 [Computer Systems Organization]: Distributed Systems; D.4.3 [Operating Systems]: File Systems Management---directory structures, distributed file systems; D.4.7 [Operating Systems]: Organization and Design. General Terms: Design, experimentation, measurement, performance, reliability Additional Key Words and Phrases: Naming, distributed system, fault tolerance, cache Authors&amp;apos; a...
Efficient Electronic Communication of Two Ruthenium Centers through a Rigid Ditopic N‐Heterocyclic Carbene Linker (2013)

A ditopic benzobis(carbene) ligand precursor was prepared that contained a chelating pyridyl moiety to ensure co-planarity of the carbene ligand and the coordination plane of a bound octahedral metal center. Bimetallic ruthenium complexes comprising this ditopic ligand [L4Ru-C,N-bbi-C,N-RuL4] were obtained by a transmetalation methodology (C,N-bbi-C,N=benzobis(N-pyridyl-N'-methyl-imidazolylidene). The two metal centers are electronically decoupled when the ruthenium is in a pseudotetrahedral geometry imparted by a cymene spectator ligand (L4=[(cym)Cl]). Ligand exchange of the Cl(-)/cymene ligands for two bipyridine or four MeCN ligands induced a change of the coordination geometry to octahedral. As a consequence, the ruthenium centers, separated through space by more than 10 Å, become electronically coupled, which is evidenced by two distinctly different metal-centered oxidation processes that are separated by 134 mV (L4=[(bpy)2]; bpy=2,2'-bipyridine) and 244 mV (L4=[(MeCN)4]), respectively. Hush analysis of the intervalence charge-transfer bands in the mixed-valent species indicates substantial valence delocalization in both complexes (delocalization parameter Γ=0.41 and 0.37 in the bpy and MeCN complexes, respectively). Spectroelectrochemical measurements further indicated that the mixed-valent Ru(II)/Ru(III) species and the fully oxidized Ru(III)/Ru(III) complexes gradually decompose when bound to MeCN ligands, whereas the bpy spectators significantly enhance the stability. These results demonstrate the efficiency of carbenes and, in particular, of the bbi ligand scaffold for mediating electron transfer and for the fabrication of molecular redox switches. Moreover, the relevance of spectator ligands is emphasized for tailoring the degree of electronic communication through the benzobis(carbene) linker.
Properties and economics (1988)

White Pigments Extender Pigments Colored Inorganic Pigments Colored Organic Pigments Black Pigments Metallic Pigments Nacreous (Pearlescent) Pigments and Interference Pigments Luminescent Organic Pigments Fluorescent and Phosphorescent Inorganic Pigments Miscellaneous Food, Drug, and Cosmetic Colors.
Theoretical analysis of a modified Koch monopole with reduced dimensions (2006)

Fractal antennas are characterised by their special geometric characteristics, which allow a reduction of the antenna dimensions. The fractal parameters and the radiative properties of modified Koch monopoles are analysed. The geometries of the antennas are obtained by an ad hoc iterative function system algorithm for fractal curve generation. Using the method of moments for numerical calculations, the influence of the antenna geometry on the resonant frequency, current, efficiency, input impedance and radiation pattern are analysed.
The Photochemistry of Lipoic Acid: Photoionization and Observation of a Triplet Excited State of a Disulfide (2005)

Abstract Under short‐wavelength UV irradiation, lipoic acid (LipSS) and its reduced form, dihydrolipoic acid (DHLA), undergo photoionization processes through a bi‐ or monophotonic pathway. After ionization, the LipSS radical cation (LipSS . + ) and radical anion (LipSS . − ) are generated. LipSS . − can be converted to equimolar amounts of LipSS and DHLA through second‐order decay. Triplet acetone can be quenched by LipSS and DHLA with a rate close to the diffusion‐controlled limit. The mechanism was further confirmed by continuous irradiation experiments. When LipSS is directly irradiated with UVA light, the first excited triplet state of LipSS is observed, with a lifetime τ =75 ns. Characteristic reactions include triplet energy transfer to oxygen and β‐carotene and addition to isoprene. The lifetime of triplet LipSS is also shortened by addition of water and methanol.
A modified microdialysis button for use in protein crystallization (2004)

A modified microdialysis button for use in protein crystallization has been designed and manufactured. This new apparatus, called the Microdialysis Rod, allows easier handling and storage, and provides versatility to the users.
Analog VLSI systems for early vision processing (2003)

Describes a project to design and build prototype analog early vision systems to determine how the advantages of analog VLSI, high speed, low power, and small area, can be exploited and its disadvantages, limited accuracy, inflexibility, lack of storage capacity, and long design times, can be minimized. This project explored three distinct circuit design styles for vision applications, continuous-time processing, charge-coupled devices, and switched capacitors. The overall effort and three chips are described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Ultrasound attenuation and thermo/photo/opto-acoustic tomography: theoretical foundation (2007)

Governing equations for ultrasonic propagation in three spatial dimensions with attenuation obeying a frequency power law are derived. Quadratic attenuation corresponds to a partial differential equation of degree four whose operator factors into a product of two parabolic operators, and impulse response is related to the heat kernel. The solutions satisfy primitive causality, but not relativistic causality. For powers that are not even integers, the waves satisfy integral-differential equations.
LPS induces late cardiac functional protection against ischemia independent of cardiac and circulating TNF-α (1997)

Lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-alpha independently induce cardioprotection against ischemia in the rat at 24 h after administration, suggesting that endogenously synthesized TNF-alpha may play a role in LPS-induced protection. The purposes of this study were 1) to delineate the time course of LPS-induced cardiac functional protection against ischemia and its relation with myocardial and circulating TNF-alpha profile, 2) to examine whether prior protein synthesis inhibition abrogates the protection, and 3) to assess the effects of TNF-alpha inhibition and neutralization on the protection. Rats were treated with LPS (0.5 mg/kg i.p.). Cardiac functional resistance to normothermic global ischemia-reperfusion was examined at sequential time points after LPS treatment in isolated hearts by the Langendorff technique. Myocardial and circulating TNF-alpha was determined by enzyme-linked immunosorbent assay at 1-24 h after LPS treatment. Protection was apparent at 24 h, 3 days, and 7 days but not at 2 or 12 h. Maximal protection at 3 days was abolished by cycloheximide pretreatment (0.5 mg/kg i.p. 3 h before LPS treatment). Increases in myocardial and circulating TNF-alpha preceded the acquisition of protection. Dexamethasone pretreatment (4.0 or 8.0 mg/kg i.p. 30 min before LPS treatment) abolished peak increase in myocardial TNF-alpha and substantially suppressed circulating TNF-alpha (54.3 and 85.9% inhibition, respectively) without an influence on the maximal protection. Similarly, maximal protection was not affected by TNF binding protein (40 or 80 microg/kg i.v. immediately after LPS treatment). The results suggest that LPS-induced cardiac functional protection against ischemia is a delayed and long-lasting protective response that may involve de novo protein synthesis. Although LPS-induced increase in myocardial and circulating TNF-alpha precedes the delayed protection, it may not be required for the delayed protection.
Automated measurement system for H2O2 in the atmosphere by diffusion scrubber sampling and HPLC analysis of Ti(iv)–PAR–H2O2 complex (2001)

An automated selective measurement system for monitoring hydrogen peroxide (H2O2) in the atmosphere was developed (using a diffusion scrubber coupled to a high performance liquid chromatography) due to the importance of H2O2 in understanding tropospheric chemistry and its harmful effects on vegetation. H2O2 in the atmosphere was effectively collected by a diffusion scrubber, which consisted of a hydrophobic porous polytetrafluorethylene (PTFE) tube positioned concentrically within a Pyrex glass tube. Titanium(IV)-4-(2-pyridylazo)resorcinol (Ti(IV)-PAR) solution acidified at pH 2.2 was used as the scrubbing solution for the diffusion scrubber. After the collection of the air sample by the diffusion scrubber, the pH value of the Ti(IV)-PAR scrubbing solution was adjusted to pH 11.9 to form a stable complex of Ti(IV)-PAR-H2O2. An aliquot of the sample solution was injected into a high performance liquid chromatograph equipped with a semi-micro-reversed-phase column and a spectrophotometric detector set at 508 nm for separating and determining the Ti(IV)-PAR-H2O2 complex. The automated measurement could be performed at 60 min intervals. The collection efficiency of H2O2 was higher than 98% at an air flow rate of 1.0 l min-1. The detection limit (3 sigma of the blank value) of H2O2 was 9 parts per trillion by volume (pptv) for an air sampling volume of 55.1. The interference from coexisting O3 or SO2 in the atmosphere was negligible during the collection of H2O2 by the diffusion scrubber. The developed automated measurement system was suitable for monitoring H2O2 in the atmosphere.
Antioxidant properties of ferulic acid dimers (1997)

Dimers of ferulic acid were chemically synthesized and the antioxidant properties assessed using (a) inhibition of ascorbate/iron-induced peroxidation of phosphatidylcholine liposomes and (b) scavenging of the radical cation of 2, 2'-azinobis (3-ethyl-benzothiazoline-6-sulphonate (ABTS) relative to the water-soluble vitamin E analogue, Trolox C (expressed as Trolox C equivalent antioxidant capacity, TEAC). The dimers examined were (E, E)-4, 4'- dihydroxy-5, 5'-dimethoxy-3, 3'-bicinnamic acid (5-5' diFA), trans-5-[(E)-2-carboxyvinyl]-2-(4-hydroxy-3-methoxy-phenyl)-7-methoxy-2, 3-dihydrobenzofuran-3-carboxylic acid (5, 8'-BenDiFA) and the methyl ester of 5, 8'-BenDiFA, dimethyl-5, 8'-BenDiFA. In both assays, the order of antioxidant efficacy was: 5, 5'-diFA > 5, 8'-BenDiFA > dimethyl-5, 8'-BenDiFA. From these results, methyl esterification decreases the antioxidant action. Comparison of the TEAC values shows that 1 mol of each of the ferulic acid dimers tested is less effective than 2 mol of free ferulic acid, and so dimerization decreases antioxidant action of these hydroxycinnamates.
Determination of the Role of Estrogen Receptors and Estrogen Regulated Genes in B Cell Autoreactivity (2009)

Abstract : Systemic lupus erythematosus (SLE) is an autoimmune disease that occurs preferentially in women. In murine models of SLE, it is clear that increased or sustained high physiologic levels of estradiol can accelerate onset of disease and exacerbate disease severity. We have shown that estradiol alters B cell maturation in vivo but does so in a genetically restricted fashion. We have also shown that estradiol can act directly on B cells to alter B cell receptor (BCR) signalling strength. This proposal is to understand which estrogen receptors mediate the effects of estradiol on B cell survival, maturation and activation in order to assess whether hormonal manipulation has a potential therapeutic role in SLE. The proposal is further designed to ask why estradiol affects B cell function in mice of one genetic background but not another.
Experimental modal analysis of radial tires and the influence of tire modes on vehicle structure borne noise (2006)

status: Published
High-reliability Pb-alloy Josephson junctions for integrated circuits (1980)

The process developed and recently used at IBM for fabricating experimental Pb-alloy Josephson tunnel-junction devices, and the factors which influence the stability of such devices during repeated cycling between 300 and 4.2 K are reviewed. A new, fine-grained Pb <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.84</inf> In <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.12</inf> Au <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.04</inf> alloy base electrode material has been developed that has excellent thermal cycling stability. In an experiment carried out to evaluate the cyclability of devices prepared with this material, excellent results were obtained: the cyclability of large-area junctions was improved by ∼100× compared to that of similar junctions prepared with the recently used, larger-grained Pb <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.84</inf> In <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.12</inf> Au <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.04</inf> base electrodes. In the best cases, populations of 2600 large junctions and 2350 interferometers were found to withstand 400 and 700 thermal cycles to 4.2 K, respectively, before the first failures were observed. These results indicate that with the use of fine-grained electrodes, Pb-alloy Josephson devices have good potential for meeting the cycling requirements of computer systems.
An ESR Study of Reversible Addition−Fragmentation Chain Transfer Copolymerization of Styrene and Maleic Anhydride (2002)

ADVERTISEMENT RETURN TO ISSUEPREVNoteAn ESR Study of Reversible Addition−Fragmentation Chain Transfer Copolymerization of Styrene and Maleic AnhydrideFu-Sheng Du, Ming-Qiang Zhu, Hai-Qing Guo, Zi-Chen Li, Fu-Mian Li, Mikiharu Kamachi, and Atsushi KajiwaraView Author Information Department of Polymer Science & Engineering, College of Chemistry, Peking University, Beijing 100871, China, and State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry, Peking University, Beijing 100871, China Department of Applied Physics and Chemistry, Fukui University of Technology, Fukui 910-8505, Japan Department of Materials Science, Nara University of Education, Nara 630-8528, Japan Cite this: Macromolecules 2002, 35, 17, 6739–6741Publication Date (Web):July 19, 2002Publication History Received11 February 2002Revised15 May 2002Published online19 July 2002Published inissue 1 August 2002https://pubs.acs.org/doi/10.1021/ma0202179https://doi.org/10.1021/ma0202179brief-reportACS PublicationsCopyright © 2002 American Chemical SocietyRequest reuse permissionsArticle Views677Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Copolymerization,Electron paramagnetic resonance spectroscopy,RAFT polymerization,Spectroscopy,Styrenes Get e-Alerts
The association of transcribed genes with the nuclear matrix ofDrosophilacells during heat shock (1985)

Using the transcriptional modulation afforded by heat shock, we found that the association of active genes with the nuclear matrix was not dependent on their level of transcription. Heat shock genes were matrix associated both before heat shock (when transcription was relatively low), and during heat shock (when transcription was greatly increased). Conversely, the cytoplasmic actin gene was matrix associated during normal growth conditions (when transcription was high) and during heat shock (when transcription was greatly decreased). Removal of greater than 99.7% of nascent RNA during preparation of the matrices did not affect these findings. Detailed examination of the cytoplasmic actin gene revealed that its matrix association was apparently mediated by multiple interactions near the 5' end of the gene.
Development and Optimization of High-Throughput Methods To MeasurePlasmodium falciparum-Specific Growth Inhibitory Antibodies (2006)

Antibodies that inhibit replication of Plasmodium falciparum in erythrocytes are thought to be important both in acquired immunity to malaria and as mediators of immunity generated by candidate blood-stage vaccines. However, several constraints have limited the study of these functional antibodies in population studies and vaccine trials. We report the development and optimization of high-throughput growth inhibition assays with improved sensitivity that use minimal volumes of test serum. The major inhibitory activity of serum from exposed donors was antibody mediated, but nonspecific inhibitory factors were found in untreated serum. Culture volumes could be effectively reduced to 25 microl to limit amounts of test serum or inhibitors used in assays. Performing inhibition assays over two cycles of parasite replication gave greater sensitivity than single-cycle assays, and a simple two-cycle inhibition assay was developed that yielded highly reproducible results. Determination of parasite growth by flow cytometry was most suitable for high-throughput assays using small culture volumes and was more sensitive than parasite lactate dehydrogenase assays and less prone to error and variation than microscopy. We evaluated and optimized methods to remove antimalarials and nonspecific inhibitory factors from serum that are suitable for use with small volumes of samples that are typically obtained from clinical studies. Both microdialysis and immunoglobulin purification by ammonium sulfate precipitation were effective and practical. These methods should facilitate evaluation of vaccine trials and clinical studies of immunity and are also suitable for testing drugs and other compounds for antimalarial activity.
Příprava purifikovaného fága S. aureus pro použití veveterinární praxi (2018)

Cilem metodiky je připrava purifikovaneho faga Staphylococcus
aureus 812h1 a využiti jeho lytických vlastnosti pro jeho přime
použiti nebo připravu nasledneho fagoveho koktejlu (spray,
tinktura apod.) využitelneho k podpůrne lecbě stafylokokových
infekci v prostředi chovu zviřat nemlecne produkce (skot, ovce,
kozy, prasata) a k osetřeni vemene dojeneho skotu, ovci a koz s
cilem omezit siřeni a přenos rezistentnich kmenů S. aureus.
Detection method for membranous proteins and their utility as a sero-diagnostic markers for lung cancer (2017)

肺がんの膜タンパク質に対する新規血清診断マーカーを開発するために，膜タンパク質を様々なプロテオーム法と組み合わせて同定した．肺癌細胞の膜タンパク質をビオチン化し，回収後，ショットガン解析により同定した．組織型の異なる4つの肺癌細胞株を用いて，免疫ブロット法と免疫染色法により同定されたタンパク質の発現を確認した．また，肺癌患者と健常患者血清を用いたReverse-phase protein array法により血清中のタンパク質レベルを測定した．CD109，CD155とRoundabout Guidance Receptor 1を含む92個の膜タンパク質が同定され，本実験ではCD155タンパク質に着目した．血清中のCD155レベルは健常者に比して肺腺癌および肺扁平上皮癌患者で有意に上昇していた（p<0.0001）．Receiver-operating characteristic curve解析を行った結果，Area under the curveは0.87であり，cut off値を0.31に設定した場合，感度と特異度はそれぞれ81％と82％であった．この方法は膜タンパク質に対する新規診断マーカーの獲得に有用であることが示された．
Pediatric-Type Indolent B-Cell Lymphomas With Overlapping Clinical, Pathologic, and Genetic Features (2022)

Pediatric-type follicular lymphoma (PTFL) and pediatric nodal marginal zone lymphoma (PNMZL) are rare pediatric-type indolent B-cell lymphomas (PedIBCL) that differ clinicopathologically from their adult counterparts. Accurate diagnosis is important to avoid overtreatment but is often challenging. The mutational landscape of PTFL is known and may aid diagnosis, but the genetic features of PNMZL are not well understood. We analyzed 21 cases of PedIBCL according to their clinicopathologic findings and classified them into PTFL (n=11), PNMZL (n=2), and "mixed type" tumors (n=8) showing ambiguous histology. We also analyzed 2 cases of adult B-cell lymphomas showing features of PedIBCL. Targeted sequencing of 121 lymphoma-related genes was performed. The median age of PedIBCL patients was 16 years (range: 3 to 47), and all but 1 PTFL patient were male. All patients presented with limited-stage disease, and only 1 relapsed. There were no significant differences in clinical features among the 3 PedIBCL groups. The most frequently mutated genes were MAP2K1 , TNFRSF14 , KMT2C , IRF8 , and NOTCH2 . The genetic features of all groups were similar to the established mutational landscape of PTFL. The 2 adult B-cell lymphomas cases also had MAP2K1 , TNFRSF14 , and IRF8 mutations, but the clinical features were not typical of PedIBCL. In summary, this study demonstrated that PTFL and PNMZL are similar diseases with overlapping clinical, pathologic, and genetic features; mixed type tumors can also occur. Atypical adult cases with similar histologic features were also observed. Therefore, the disease spectrum of PedIBCL may be much broader than is currently believed.