gebhart/patrepeval huggingface dataset:
Abstract 1870: The anti-proliferative and pro-apoptotic effect of MDM2-p53 antagonists evaluated in human tumor cells lines and chronic lymphocytic leukemia patient samples (2018)

Abstract We investigated the cellular response to two MDM2-p53 antagonists (RG7388 and Cpd 1) in a panel of 19 tumor cell lines and in patient-derived chronic lymphocytic leukemia (CLL) cells. RG7388 (Idasanutlin) is an MDM2-p53 antagonist developed by Roche and Cpd1 a novel potent and selective MDM2-p53 antagonist developed as part of an ongoing Alliance between Newcastle University, Cancer Research UK and Astex Pharmaceuticals. RG7388 and Cpd1 potently inhibited the proliferation of many cell lines, with MDM2-amplified SJSA-1 osteosarcoma cells and cells from haematological malignancies being the most sensitive (e.g. GI50 values in the acute myeloid leukemia cell line MOLM13 were 33 + 16 nM for RG7388 and 8 + 1 nM for Cpd 1). We also examined induction of apoptosis by measuring caspase 3/7 activation (24 h treatment). SJSA-1 cells showed a 20-fold increase in caspase activation and Molm13 cells a 6-fold increase (measured at 300nM with RG7388). However, cell lines from solid tumours such as the colorectal carcinoma HCT116 or the hepatocellular carcinoma HepG2 did not show any induction of apoptosis, even at concentrations up to 1µM RG3788. In primary CLL cells, Cpd 1 reduced cell viability (48 h) with an average LC50 of 131 + 46 nM (n = 4), compared to 400 + 55 nM for RG7388 (n =23). We also quantified mRNA levels in 25 primary CLL samples and found that RG7388 induced a predominantly pro-apoptotic gene signature: following 6h treatment, 1µM RG7388 resulted in an average 8-fold induction of PUMA and 3.5-fold increase in BAX compared to a 1.6-fold induction of CDKN1A (p21). Our data confirm that targeting the MDM2-p53 interaction is an effective strategy to inhibit growth and viability of tumor cells, and that some cells, including those expressing high levels of MDM2 or those derived from hematological malignancies, display a striking apoptotic response. Citation Format: Elaine Willmore, Yan Zhao, Carmela Ciardullo, Laura Woodhouse, Huw D. Thomas, Maria Ahn, Lynsey Fazal, Martin E. Noble, Ian Hardcastle, Steven Howard, Gianni Chessari, John Lunec, Steve Wedge. The anti-proliferative and pro-apoptotic effect of MDM2-p53 antagonists evaluated in human tumor cells lines and chronic lymphocytic leukemia patient samples [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 1870.
Sensitivity to Lovastatin of <i>Saccharomyces cerevisiae</i> Strains Deleted for Pleiotropic Drug Resistance (<i>PDR)</i> Genes (2011)

The use of statins is well established in human therapy, and model organisms such as Saccharomyces cerevisiae are commonly used in studies of drug action at molecular and cellular levels. The investigation of the resistance mechanisms towards statins may suggest new approaches to improve therapy based on the use of statins. We investigated the susceptibility to lovastatin of S. cerevisiae strains deleted for PDR genes, responsible for exporting hydrophobic and amphiphilic drugs, such as lovastatin. Strains deleted for the genes tested, PDR1, PDR3, PDR5 and SNQ2, exhibited remarkably different phenotypes, with deletion of PDR5 causing the highest sensitivity to lovastatin. The study helped clarifying which pdr mutants to use in studies of physiological actions of statins in yeast.
SPECC1L regulates palate development downstream of IRF6 (2020)

SPECC1L mutations have been identified in patients with rare atypical orofacial clefts and with syndromic cleft lip and/or palate (CL/P). These mutations cluster in the second coiled-coil and calponin homology domains of SPECC1L and severely affect the ability of SPECC1L to associate with microtubules. We previously showed that gene-trap knockout of Specc1l in mouse results in early embryonic lethality. We now present a truncation mutant mouse allele, Specc1lΔC510, that results in perinatal lethality. Specc1lΔC510/ΔC510 homozygotes showed abnormal palate rugae but did not show cleft palate. However, when crossed with a gene-trap allele, Specc1lcGT/ΔC510 compound heterozygotes showed a palate elevation delay with incompletely penetrant cleft palate. Specc1lcGT/ΔC510 embryos exhibit transient oral epithelial adhesions at E13.5, which may delay shelf elevation. Consistent with oral adhesions, we show periderm layer abnormalities, including ectopic apical expression of adherens junction markers, similar to Irf6 hypomorphic mutants and Arhgap29 heterozygotes. Indeed, SPECC1L expression is drastically reduced in Irf6 mutant palatal shelves. Finally, we wanted to determine if SPECC1L deficiency also contributed to non-syndromic (ns) CL/P. We sequenced 62 Caucasian, 89 Filipino, 90 Ethiopian, 90 Nigerian and 95 Japanese patients with nsCL/P and identified three rare coding variants (p.Ala86Thr, p.Met91Iso and p.Arg546Gln) in six individuals. These variants reside outside of SPECC1L coiled-coil domains and result in milder functional defects than variants associated with syndromic clefting. Together, our data indicate that palate elevation is sensitive to deficiency of SPECC1L dosage and function and that SPECC1L cytoskeletal protein functions downstream of IRF6 in palatogenesis.
Behaviors of 90° and 180° Domain Walls under <i><b>c</b></i>-axis Polarization Switching in Ferroelectric Bi<sub>4</sub>Ti<sub>3</sub>O<sub>12</sub> Single Crystals (2009)

We present the behaviors of 90° and 180° domain walls under applying an electric field along the c axis in monoclinic Bi4Ti3O12 crystals. Piezoresponse force microscope investigations taking into account the two spontaneous polarization (Ps) components along the a (Ps(a)) and c (Ps(c)) axes reveal that 90° domain walls composed of the Ps(a) component impede the switching of the Ps(c) domains, leading to a lower remanent polarization along the c axis. 180° domain walls are found to be transformed from a curved configuration into a faceted configuration. These behaviors of Ps(a) domain walls raise the possibility of achieving material design using domain engineering for piezoelectric and optical applications of Bi4Ti3O12
Cloned horse pregnancies produced using adult cumulus cells (2004)

The objectives of the present study were to: (1) clone horses using adult cumulus cells; and (2) determine whether the cumulus cell donor affected the outcome. In vivo-matured cumulus-oocyte complexes were obtained using transvaginal ultrasound-guided follicle aspiration; oocytes were used as cytoplasts, whereas cumulus cells (from one of three different mares) were used as donor cells. Immediately following nuclear transfer and activation procedures, cloned embryos were transferred surgically to the oviduct of recipient mares (n = 2-5 embryos per recipient) that had ovulated within 24 h prior to the transfer. An initial pregnancy examination was performed between Days 14 and 16 (Day 0 = surgery); subsequent examinations were then performed every 7-10 days. A total of 136 follicles were aspirated in 96 mares, from which 72 oocytes were recovered (53%). Sixty-two cloned embryos were transferred to recipient mares, which resulted in seven (11.3%) ultrasonographically detectable conceptuses between Days 14 and 16. All seven conceptuses were lost spontaneously between Days 16 and 80. Cumulus cells from Mare 160 tended (P = 0.08) to result in a higher embryo survival rate than cumulus cells from Mare 221 (4/17 v. 1/25 respectively). To our knowledge, this is the first report documenting the establishment of cloned equine pregnancies derived from adult cumulus cells.
UNDERSTANDING NEAR-SURFACE TURBULENCE FLUXES USING AN OPEN- PATH GAS ANALYSER (2010)

The concentrations of carbon dioxide (CO2) and water vapour were measured over 9 weeks on the Atmospheric Observatory at the University of Reading using an open-path gas analyser (OPGA). Fluxes were estimated from these measurements, combined with data from a sonic anemometer, using the eddy correlation technique. The instrument measurement error was 0.30 % on concentrations of CO2 and 1.3 % on water vapour. Concentrations of CO2 were approximately 30 ppmv higher at night than during the day and correspond to positive CO2 fluxes at night and negative fluxes during the day. This is characteristic of a vegetated area and the diurnal variations are related to the atmospheric boundary layer depth as well as respiration and absorption of CO2 through photosynthesis. A source area analysis was not inconsistent with gas concentrations and particularly fluxes, originating mainly from a vegetated area within 100 m from the sensor. The absolute humidity, as measured with the OPGA, correlated well with results from a psychrometric measurement and small differences are attributed to the choice of the coefficients used in psychrometric method. The latent heat flux derived from the eddy correlation method was up to 8 times smaller than fluxes derived from open-pan, Piche tube or Penman-Monteith evaporation. The eddy correlation measurements indicate evaporation restricted by moisture availability, mobility, plant physiology and atmospheric conditions while the other methods represent unrestricted evaporation. Uncertainties associated with each method lead to further differences. The hypothesis is put forward that the uncertainty associated with the eddy correlation method originates mainly from post-field processing. Although the measurements were carried out in an urban environment, the results are only representative of the vegetated surroundings within 100 m from the sensor and indicate the importance of intra-urban variation in surface characteristics as well as the need to select appropriate measurement locations to obtain results that are representative of the wider
Increasing Oil Recovery in Naturally-Fractured Carbonate Reservoirs Using Microemulsions (2021)

Summary Enhanced Oil Recovery (EOR) is nowdays gaining more attention than any time before. EOR methods have been applied world widely to sandstone reservoirs and proved to increase oil recovery factor. So far Naturally fractured carbonate Reservoirs (NFCRs) have showed short life time production with low ultimate recovery factor during primary recovery stage. The oil produced at surface depleting natural reservoir energy is drained mainly from the fracture system where 90% of the oil in matrixes remains trapped in. Towards treating this situation, there has been extensive research in oil/brine/rock systems as well as natural and chemical emulsion formation at conventional reservoir conditions. However, no experimental simulation study has investigated the dynamic fluid flow and advancement between matrix and fracture system for NFCR. In this study a laboratory micro-model carbonate has been designed to simulate and evaluate the dynamic flow of chemical emulsion and reservoir fluids advance in fractures and matrixes of carbonate reservoirs during the injection of surface-active agent. The findings demonstrated that viscous forces as well as increased viscosity in the microemulsion lead to better mobility control which can substantially increase oil production in NFC reservoirs.
Consequences of Amide Connectivity in the Supramolecular Polymerization of Porphyrins: Spectroscopic Observations Rationalized by Theoretical Modelling (2021)

The correlation between molecular structure and mechanism of supramolecular polymerizations is a topic of great interest, with a special focus on the pathway complexity of porphyrin assemblies. Their cooperative polymerization typically yields highly ordered, long 1D polymers and is driven by a combination of π-stacking due to solvophobic effects and hydrogen bonding interactions. Subtle changes in molecular structure, however, have significant influence on the cooperativity factor and yield different aggregate types (J- versus H-aggregates) of different lengths. In this study, the influence of amide connectivity on the self-assembly behavior of porphyrin-based supramolecular monomers was investigated. While in nonpolar solvents, C=O centered monomers readily assemble into helical supramolecular polymers via a cooperative mechanism, their NH centered counterparts form short, non-helical J-type aggregates via an isodesmic pathway. A combination of spectroscopy and density functional theory modelling sheds light on the molecular origins causing this stunning difference in assembly properties and demonstrates the importance of molecular connectivity in the design of supramolecular systems. Finally, their mutual interference in copolymerization experiments is presented.
Towards Gaussian Multi-Robot SLAM for Underwater Robotics (2005)

The international recommendations on nutrition in the ICU should be followed. Some specific issues about the nutrition of the COVID-19 patients in the ICU should be emphasized. Universal infection prevention precautions (hand hygiene and use of personal protection equipment (PPE)) are invaluable during nursing of COVID 19 patients at ICU.
La autoridad en la familia (2014)

Las nuevas relaciones sociales han dado a luz a un modelo de familia negociadora, en el que sus miembros consensuan un proyecto de vida basado en la libertad, la responsabilidad y la autonomia. La autoridad se gana en la cercania, con argumentos basados en la razon y los limites que dan confianza.
Monoclonal Antibodies to Molluskan Hemocyanin from Concholepas concholepas Demonstrate Common and Specific Epitopes among Subunits (2002)

We studied the reactivity of mouse monoclonal antibodies (MAbs) against the hemocyanin from the Chilean marine gastropod Concholepas concholepas (CCH). This protein has been successfully used as a carrier to produce antibodies to haptens and peptides. All MAbs (13) belonging to IgG subclass exhibit dissociation constants (K(d)) from 1 x 10(-7) M to 1 x 10(-9) M. MAbs were characterized by enzyme-linked immunosorbant assay (ELISA) using CCH treated with different procedures, including dissociation into CCH-A and CCH-B subunits, Western blot, enzymatic digestion, chemical deglycosylation, and thermal denaturation. MAbs were classified into three categories, according to subunit specificity by ELISA. The epitope distribution shows that CCH subunits display common epitopes (group I, 5 MAbs, 1H5, 2A8, 3A5, 3B3, and 3E3), as well as specific epitopes for CCH-A subunits (group II, 3 MAbs, 1B8, 4D8, and 8E5) and for CCH-B subunits (group III, 5 MAbs, 1A4, 1E4, 2H10, 3B7, and 7B4). The results can be summarized as follows: (1). six antibodies react with thermal denatured CCH, suggesting that they recognize linear epitopes, whereas seven recognize conformational epitopes; (2). oxidation of carbohydrate moieties does not affect the binding of the MAbs; (3). enzymatic digestion of CCH decreases the reactivity of all antibodies irrespective of the protease used (elastase or trypsin); (4). bringing together the above data, in addition to epitopic complementarity analysis, we identified 12 different epitopes on the CCH molecule recognized by these MAbs. The anti-CCH MAbs presented here can be useful tools to understand the subunit organization of the CCH and its complex structure, which can explain its immunogenic and immunostimulating properties in mammals.
Spatial and temporal communication theory using adaptive antenna array (1998)

An adaptive antenna array or a smart antenna is named a software antenna because it can form a desired antenna pattern and adaptively control it if an appropriate set of antenna weights is provided and updated in software. It can be a typical tool for realizing a software radio. An adaptive antenna array can be considered an adaptive filter in the space and time domains for radio communications, so communication theory can be generalized from a conventional time domain into both space and time domains. This article introduces a spatial and temporal communication theory based on an adaptive antenna array, such as spatial and temporal channel modeling, equalization, optimum detection for single-user and multi-user CDMA, precoding in a transmitter, and joint optimization of both transmitter and receiver. Such spatial and temporal processing promises significant improvement of performance against multipath fading in mobile radio communications.
Active acquisition of 3D shape for moving objects (2002)

An active 3D acquisition system is presented that projects a simple pattern of squares on a scene and views it from a different angle. This paper describes how the observed pattern can be extracted from the image data. The underlying algorithm automatically detects the lines and crossings of the projected pattern in the image. Experiments show that the algorithm is robust and provides accurate three-dimensional (3D) reconstructions. Its one-shot operation principle enables the system to retrieve the shape of moving objects.
Extended Hormone Binding Site of the Human Thyroid Stimulating Hormone Receptor (2008)

The human thyroid stimulating hormone receptor (hTSHR) belongs to the glycoprotein hormone receptors that bind the hormones at their large extracellular domain. The extracellular hinge region of the TSHR connects the N-terminal leucine-rich repeat domain with the membrane-spanning serpentine domain. From previous studies we reasoned that apart from hormone binding at the leucine-rich repeat domain, additional multiple hormone contacts might exist at the hinge region of the TSHR by complementary charge-charge recognition. Here we investigated highly conserved charged residues in the hinge region of the TSHR by site-directed mutagenesis to identify amino acids interacting with bovine TSH (bTSH). Indeed, the residues Glu-297, Glu-303, and Asp-382 in the TSHR hinge region are essential for bTSH binding and partially for signal transduction. Side chain substitutions showed that the negative charge of Glu-297 and Asp-382 is necessary for recognition of bTSH by the hTSHR. Multiple combinations of alanine mutants of the identified positions revealed an increased negative effect on hormone binding. An assembled model suggests that the deciphered acidic residues form negatively charged patches at the hinge region resulting in an extended binding mode for bTSH on the hTSHR. Our data indicate that certain positively charged residues of bTSH might be involved in interaction with the identified negatively charged amino acids of the hTSHR hinge region. We demonstrate that the hinge region represents an extracellular intermediate connector for both hormone binding and signal transduction of the hTSHR.
Reactor Developments in Hydrotreating and Conversion of Residues (2018)

A spectrum of hydrogen-addition processes is available. Many similar fixed-bed trickle reactor processes have been developed and can be licensed from such companies as Shell, Gulf, and Exxon. Due to the catalyst bed expansion, the volumetric catalyst hold-up per reactor volume is smaller than in trickle-bed reactors. Ebullating-bed reactors–as in the H-Oil or the LC-Fining processes–are commercially applied in various residue upgrading projects. Both trickle-bed and ebullating-bed reactors for residue upgrading applications are expensive capital cost items because of the high temperatures and pressures applied and the huge catalyst volumes required. The emerging slurry-phase technologies will necessitate a few more years of development, primarily due to the limited understanding of the factors that influence the hydrodynamic behavior of the reactor system. Reactor temperature control is important in ebullating-bed reactors, as is reflected in the great number of thermocouples installed in the reactor.
A method for the controllable fabrication of optical fiber-based localized surface plasmon resonance sensors (2022)

Optical fiber-based Localized Surface Plasmon Resonance (OF-LSPR) biosensors have emerged as an ultra-sensitive miniaturized tool for a great variety of applications. Their fabrication by the chemical immobilization of gold nanoparticles (AuNPs) on the optic fiber end face is a simple and versatile method. However, it can render poor reproducibility given the number of parameters that influence the binding of the AuNPs. In order to develop a method to obtain OF-LSPR sensors with high reproducibility, we studied the effect that factors such as temperature, AuNPs concentration, fiber core size and time of immersion had on the number and aggregation of AuNPs on the surface of the fibers and their resonance signal. Our method consisted in controlling the deposition of a determined AuNPs density on the tip of the fiber by measuring its LSPR signal (or plasmonic signal, Sp) in real-time. Sensors created thus were used to measure changes in the refractive index of their surroundings and the results showed that, as the number of AuNPs on the probes increased, the changes in the Sp maximum values were ever lower but the wavelength shifts were higher. These results highlighted the relevance of controlling the relationship between the sensor composition and its performance.
Persee: a nulling demonstrator with real-time correction of external disturbances (2008)

Nulling interferometry is one of the most promising methods to study habitable extrasolar systems. Several projects, such as Darwin, TPF, Pegase, FKSI or Aladdin, are currently considered and supported by R&D programs. One of the main issues of nulling interferometry is the feasibility of a stable polychromatic null despite the presence of significant disturbances, induced by vibrations, atmospheric turbulence on the ground or satellite drift for spaceborne missions. To reduce cost and complexity of the whole system, it is necessary to optimize not only the control loop performance at platform and payload levels, but also their interaction. In this goal, it was decided in 2006 to build a laboratory demonstrator named Persee. Persee is mostly funded by CNES and built by a consortium including CNES, IAS, LESIA, OCA, ONERA and TAS. After a definition phase in 2006, the implementation of the sub-systems has now begun and the integration near Paris by GIS-PHASE (LESIA, ONERA and GEPI) is planned in 2009. This paper details the main objectives of PERSEE, describes the definition of the bench, presents the current status and reports results obtained with the first sub-systems.