gebhart/patrepeval huggingface dataset:
Bispecific therapeutics for ophthalmic indications: target selection and the optimal molecular format (2014)

The ocular compartment presents distinct challenges for drug delivery. For the scope of this review, the authors focus on two specific diseases of the posterior segment of the eye including age related macular degeneration (AMD) and diabetic macular edema for which current successful treatments include protein-based molecules delivered into the eye via repeat intravitreal injection. Distinct from the neovascular form of AMD, a large unmet need remains in the advanced dry form of AMD. In consideration of the next wave of biotherapeutics for intraocular application, combination targeted agents are expected. Here the authors review technologies to engineer dual targeting into protein-based therapeutics for intravitreal delivery with an additional aim of minimizing systemic exposure after intraocular injection. In the summary the authors highlight two of these forms, DAPRin and Fab’2, as important to monitor closely as bispecifics for ocular therapy development.
Glutathione conjugation and pharmacokinetics of 2‐bromo‐3‐phenylpropionic acid in vitro and in the rat in vivo (1992)

Glutathione (GSH) conjugation of the chiral compound 2-bromo-3-phenylpropionic acid (BPP) was studied in vitro and in the rat in vivo. GSH conjugation of BPP, catalyzed by a mixture of glutathione-S-transferases (GST's) from rat liver cytosol in vitro, was stereoselective: at a substrate concentration of 250 microM, (R)-BPP was more rapidly conjugated than (S)-BPP (R/S-ratio = 2.6). The blood elimination kinetics of the separate BPP enantiomers and the biliary excretion kinetics of the corresponding GSH conjugates were studied in the rat in vivo after administration of (R)- or (S)-BPP at a dose level of 50 mumol/kg. Elimination of (R)-BPP from blood was faster than that of (S)-BPP: half lives were 9 +/- 2 min for (R)-BPP and 13 +/- 1 min for (S)-BPP. The biliary excretion rate of the GSH conjugate of (R)-BPP declined monoexponentially, while that of the GSH conjugate of (S)-BPP displayed a biphasic profile. Half lives of excretion were 13 +/- 1 for the GSH conjugate of (R)-BPP, and 11 +/- 2 for the GSH conjugate of (S)-BPP (second phase). The first phase in the biliary excretion of the GSH conjugate of (S)-BPP could not be attributed to capacity limitation of biliary transport carriers as higher excretion rates were attained upon administration of higher doses (100 and 200 mumol/kg) of (S)-BPP). The blood elimination profiles of (R)- and (S)-BPP differed greatly from the biliary excretion profiles of the corresponding GSH conjugates. This suggests that the kinetics of BPP conjugate excretion are determined by other processes than hepatic GSH conjugation.
Phosphorylation stabilizes Nanog by promoting its interaction with Pin1 (2010)

Embryonic stem cells (ESCs) can undergo unlimited self-renewal and retain the pluripotency to differentiate into all cell types in the body, thus holding great promise as a renewable source of cells for human therapy. The mechanisms that maintain self-renewal of ESCs remain unclear. Here we show that Nanog, a transcription factor crucial for the self-renewal of ESCs, is phosphorylated at multiple Ser/Thr-Pro motifs. This phosphorylation promotes the interaction between Nanog and the prolyl isomerase Pin1, leading to Nanog stabilization by suppressing its ubiquitination. Inhibition of Pin1 activity or disruption of Pin1-Nanog interaction in ESCs suppresses their capability to self-renew and to form teratomas in immunodeficient mice. Therefore, in addition to the stringent transcriptional regulation of Nanog, the expression level of Nanog is also modulated by posttranslational mechanisms.
La producción didáctica en el campo de la fisica en el NURR-ULA desde las perspectivas de las tic (2011)

Internet use for education is the global communication between students or students, teachers and facilitators or subject matter experts to create a climate of collaborative and interactive feel the student is not alone in its diffi culties. The teaching of a particular program content using multimedia devices, is a new way of understanding the educational process, creating new forms of socialization and even defi nitions of individual and collective identity, diversifying the sources of knowledge and wisdom. For these reasons, it was considered appropriate to guide the aim of this study to the question about the educational software, its features, evaluation and implementation substantially in the fi eld of physics under the NURR-ULA, in order to meet the application and projection of each of them and the context in which they were made, if whether these arise from a teaching experience with program content of a curriculum for its development, or simply a games and entertainment software content-based teaching learning training purposes only. Whatever the case, these tools must meet the requirements of validity, technical and graphic design, which determine the quality and effi ciency as a learning tool. In this respect are presented and analyzed various educational software, which have been developed or advised by researchers in our group with the technique of Web pages for teaching and learning of some topics in Physics and Mathematics
Dynamics of Dual Frequency Mode-Locked Fiber Lasers (2018)

The nonlinear dynamics of a dual-frequency mode-locked fiber laser was investigated theoretically. Cross phase modulation between the two frequency channels induces a new gain loss dynamics which leads to unequal energy pulses in two channels.
Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus (2018)

Aspiculuris tetraptera is a heteroxynematid nematoda infecting most of the laboratory animals, occasionally mice which represent the mostly used animal for biological, medical, and pharmacological studies. The present study aimed to investigate the prevalence of nematode parasites infection in the laboratory mice Mus musculus in Egypt. Morphologically, this oxyurid possessed four distinct cephalic papillae on a cephalic plate, with three small rudimental lips carrying two sessile poorly developed labial papillae and one pair of amphidial pores. Esophagus divided into cylindrical corpus and globular bulb. Distinct cervical alae interrupted at the level of esophago-intestinal junction forming an acute angle. At the caudal end, twelve caudal papillae in male worms while an ovijector apparatus opening and a vulva surrounded by protruded lips in females were observed. The general morphological criteria include this nematode with other Aspiculuris species which were compared in the present study. Molecular characterization based on 18SSU rDNA sequencing performed to confirm the taxonomic position of this species and to documents the morphological data. Sequence alignment detects a percent of identity up to 88.0% with other Heteroxynematidae species. Phylogenetic analysis showed that the present recorded is a putative sister taxon to A. tetraptera recorded in a previous study. The SSU rDNA sequence has been deposited in the GenBank under the accession no. MG019400.
Broad-range bacterial polymerase chain reaction for identification of bacteria in inflamed joints: comment on the article by Wilbrink et al (1998)

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Dietary Supplementation of Chinese Ginseng Prevents Obesity and Metabolic Syndrome in High-Fat Diet-Fed Mice (2014)

Obesity and diabetes are growing health problems worldwide. In this study, dietary provision of Chinese ginseng (0.5 g/kg diet) prevented body weight gain in high-fat (HF) diet-fed mice. Dietary ginseng supplementation reduced body fat mass gain, improved glucose tolerance and whole body insulin sensitivity, and prevented hypertension in HF diet-induced obese mice. Ginseng consumption led to reduced concentrations of plasma insulin and leptin, but had no effect on plasma adiponectin levels in HF diet-fed mice. Body temperature was higher in mice fed the ginseng-supplemented diet but energy expenditure, respiration rate, and locomotive activity were not significantly altered. Dietary intake of ginseng increased fatty acid oxidation in the liver but not in skeletal muscle. Expression of several transcription factors associated with adipogenesis (C/EBPα and PPARγ) were decreased in the adipose tissue of HF diet-fed mice, effects that were mitigated in mice that consumed the HF diet supplemented with ginseng. Abundance of fatty acid synthase (FASN) mRNA was greater in the adipose tissue of mice that consumed the ginseng-supplemented HF diet as compared with control or un-supplemented HF diet-fed mice. Ginseng treatment had no effect on the expression of genes involved in the regulation of food intake in the hypothalamus. These data suggest that Chinese ginseng can potently prevent the development of obesity and insulin resistance in HF diet-fed mice.
Proof of concept of volumetric MR-temperature monitoring during microwave ablation of liver tumor in a patient (2023)

Microwave tumor ablation in the liver is commonly performed in computed tomography scanners with limited imaging capability for real-time monitoring of treatment progression. Performing this procedure under MRI enables to monitor temperature and thermal dose during the ablation. It allows better understanding of the lesion creation and gives better insight of the treatment outcome. In this study, we evaluate a complete MR-guided workflow that include volumetric temperature and dose monitoring during ablation using an ultrafast EPI sequence with respiratory triggering. Results show that spatial extent of the lesion can be monitored and displayed in real-time.
Development of novel instrumentation and expert system concepts in turbine generator condition monitoring (1989)

Efforts to develop new diagnostic techniques to improve condition monitoring of turbine generators are described. Work is concentrated on those areas where deficiencies in existing instrumentation were identified. Improvements in assessment capability are also being sought through reduction in the false alarm rate of existing techniques and by better use of conventional sensor data by means of expert systems concepts. >
Supplementary Table 8 from Integrated Genomic Profiling of Chronic Lymphocytic Leukemia Identifies Subtypes of Deletion 13q14 (2023)

Supplementary Table 8 from Integrated Genomic Profiling of Chronic Lymphocytic Leukemia Identifies Subtypes of Deletion 13q14
Exploiting geo-social correlations to improve pairwise ranking for point-of-interest recommendation (2018)

Recently, as location-based social network (LBSN) rapidly grow, point-of-in-terest (POI) recommendation has become an important way to help people locate interesting places. Nowadays, there have been deep studies conducted on the geographical and social influence in the point-of-interest recommendation model based on the rating prediction. The fact is, however, relying solely on the rating fails to reflect the user's preferences very accurately, because the users are most concerned with the list of ranked point-of-in-terests (POIs) on the actual output of recom-mender systems. In this paper, we propose a co-pairwise ranking model called Geo-Social Bayesian Personalized Ranking model (GSB-PR), which is based on the pairwise ranking with the exploiting geo-social correlations by incorporating the method of ranking learning into the process of POI recommendation. In this model, we develop a novel BPR pairwise ranking assumption by injecting users' geo-social preference. Based on this assumption, the POI recommendation model is reformulated by a three-level joint pairwise ranking model. And the experimental results based on real datasets show that the proposed method in this paper enjoys better recommendation performance compared to other state-of-the-art POI recommendation models.
[Acute embolic myocardial infarction complicating bioprosthetic stentless aortic valve endocarditis]. (2003)

We describe the case of bioprosthetic stentless aortic valve endocarditis due to Staphylococcus epidermidis in a female patient, who previously underwent mitral and aortic valve replacement: she presented left main coronary artery occlusion due to septic embolus with subsequent myocardial infarction complicated by cardiogenic shock. We discuss the therapeutic strategies, both medical and interventional, and the surgical options.
EAACI Position Paper: Prevention of work-related respiratory allergies among pre-apprentices or apprentices and young workers (2011)

Apprenticeship is a period of increased risk of developing work-related respiratory allergic diseases. There is a need for documents to provide appropriate professional advice to young adults aiming to reduce unsuitable job choices and prevent impairment from their careers. The present document is the result of a consensus reached by a panel of experts from European and non-European countries addressed to allergologists, pneumologists, occupational physicians, primary care physicians, and other specialists interested in this field, which aims to reduce work-related respiratory allergies (rhinoconjunctivitis and asthma) among allergic or nonallergic apprentices and other young adults entering the workforce. The main objective of the document is to issue consensus suggestions for good clinical practice based on existing scientific evidence and the expertise of a panel of physicians.
Changes in sputum bacterial density in people with bronchiectasis: BRONCH UK Study data (2021)

<b>Introduction:</b> We investigated (i) changes in total bacterial density in sputum during clinical stability and PEx and (ii) the relationship between total bacterial density, inflammation and FEV<sub>1</sub>. <b>Methods:</b> Two subgroups from the BRONCH-UK cohort study (n=74) were analysed; A) clinically stable patients (n=54) who provided sputum at baseline and one further visit within 12 months; B) PEx patients (n=20) who provided sputum at baseline, pre- and post-Abx treatment of an PEx and one further visit within 12 months when clinically stable. Total bacterial density (16S rRNA) was determined by qPCR and patients were stratified as having moderate (10<sup>5</sup>- &lt;10<sup>7</sup> copies/ml) or high bacterial (≥10<sup>7</sup> copies/ml) density. Non-parametric tests were used for analysis. <b>Results:</b> Of 74 patients, 58% female, mean (SD) age 66 (8.7) years, FEV<sub>1</sub> 67 (20.5) %. At baseline, 23/54 patients (43%) in the stable group had high bacterial density vs. 7/20 patients (35%) in the PEx group. No difference was observed in age, FEV<sub>1</sub> or CRP in patients with moderate vs. high bacterial density (p&gt;0.05). Between baseline and final stable visit, there was no difference in bacterial density (median Log<sub>10</sub> 16S rRNA 6.8 [4.9-7.9]&nbsp;vs. 6.7 [5.2-8.0] copies/ml, respectively; p= 0.58) and no significant change in the number of patients with high or moderate bacterial density. No difference was observed in bacterial density pre and post Abx treatment (median Log<sub>10</sub> 16S rRNA 6.9 [5.4-8.0] vs 7.1 [5.7-8.1] copies/ml, respectively; p=0.28). Bacterial density did not correlate with FEV<sub>1</sub> (r=0.11; p=0.37) or CRP (r=0.10; p=0.40) at baseline. <b>Conclusion:</b> Bacterial density was stable during clinical stability and did not change following Abx therapy of a PEx.
EML4 promotes the loading of NUDC to the spindle for mitotic progression (2015)

Echinoderm microtubule-associated protein (EMAP)-like (EML) family proteins are microtubule-associated proteins that have a conserved hydrophobic EMAP-like protein (HELP) domain and multiple WD40 domains. In this study, we examined the role of EML4, which is a member of the EML family, in cell division. Time-lapse microscopy analysis demonstrated that EML4 depletion induced chromosome misalignment during metaphase and delayed anaphase initiation. Further analysis by immunofluorescence showed that EML4 was required for the organization of the mitotic spindle and for the proper attachment of kinetochores to microtubules. We searched for EML4-associating proteins by mass spectrometry analysis and found that the nuclear distribution gene C (NUDC) protein, which is a critical factor for the progression of mitosis, was associated with EML4. This interaction was mediated by the WD40 repeat of EML4 and by the C-terminus of NUDC. In the absence of EML4, NUDC was no longer able to localize to the mitotic spindle, whereas NUDC was dispensable for EML4 localization. Our results show that EML4 is critical for the loading of NUDC onto the mitotic spindle for mitotic progression.
Gene Targeting in Disease Networks (2021)

Profiling of whole transcriptomes has become a cornerstone of molecular biology and an invaluable tool for the characterization of clinical phenotypes and the identification of disease subtypes. Analyses of these data are becoming ever more sophisticated as we move beyond simple comparisons to consider networks of higher-order interactions and associations. Gene regulatory networks (GRNs) model the regulatory relationships of transcription factors and genes and have allowed the identification of differentially regulated processes in disease systems. In this perspective, we discuss gene targeting scores, which measure changes in inferred regulatory network interactions, and their use in identifying disease-relevant processes. In addition, we present an example analysis for pancreatic ductal adenocarcinoma (PDAC), demonstrating the power of gene targeting scores to identify differential processes between complex phenotypes, processes that would have been missed by only performing differential expression analysis. This example demonstrates that gene targeting scores are an invaluable addition to gene expression analysis in the characterization of diseases and other complex phenotypes.
A Study of Liveness Detection in Face Biometric Systems (2014)

Biometrics" refers to the technologies that measure and analyze human body characteristics for security purposes. The need of privacy and security in our daily life leads to this new area. Biometric systems have more accuracy when compared to traditional methods (password, key etc). It identifies and verifies the identity of a person based on one or more physiological and behavioral characteristics. That is Human body as password. The most common physical biometric traits includes fingerprint, face, ear, iris, retina, hand geometry, palmprint, DNA etc. Behavioral biometric traits include signature, gait, key strokes, speech patterns etc. Each biometric has its own strength and limitations and accordingly each biometric is used in identification (authentication) application. This paper concentrates on spoof attack against face recognition system, i.e. in this type of attack a fake biometric can be presented to sensor.
Mechanical circulatory support therapy as a bridge to transplant or recovery (new advances) (2006)

Large pulsatile left ventricular assist devices have been the mainstay of mechanical support. The combination of durability and smaller pump size has become a focus of mechanical circulatory support in the current era. Ongoing clinical trials in the United States with three rotary pumps in both bridge-to-transplant and destination trials will likely result in a major increase in options for circulatory support. At least five other small, durable devices have recently entered non-US trials and are poised for clinical studies in the US. The National Institutes of Health-sponsored Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS) will facilitate the introduction of new technology and study patient and device outcomes.
Stress-induced glucocorticoid signaling remodels neurovascular coupling through impairment of cerebrovascular inwardly rectifying K + channel function (2014)

Studies of stress effects on the brain have traditionally focused on neurons, without considering the cerebral microcirculation. Here we report that stress impairs neurovascular coupling (NVC), the process that matches neuronal activity with increased local blood flow. A stressed phenotype was induced in male rats by administering a 7-d heterotypical stress paradigm. NVC was modeled by measuring parenchymal arteriole (PA) vasodilation in response to neuronal stimulation in amygdala brain slices. After stress, vasodilation of PAs to neuronal stimulation was greatly reduced, and dilation of isolated PAs to external K(+) was diminished, suggesting a defect in smooth muscle inwardly rectifying K(+) (KIR) channel function. Consistent with these observations, stress caused a reduction in PA KIR2.1 mRNA and smooth muscle KIR current density, and blocking KIR channels significantly inhibited NVC in control, but not in stressed, slices. Delivery of corticosterone for 7 d (without stressors) or RU486 (before stressors) mimicked and abrogated NVC impairment by stress, respectively. We conclude that stress causes a glucocorticoid-mediated decrease in functional KIR channels in amygdala PA myocytes. This renders arterioles less responsive to K(+) released from astrocytic endfeet during NVC, leading to impairment of this process. Because the fidelity of NVC is essential for neuronal health, the impairment characterized here may contribute to the pathophysiology of brain disorders with a stress component.
Data from Development of Real-time Subcellular Dynamic Multicolor Imaging of Cancer-Cell Trafficking in Live Mice with a Variable-Magnification Whole-Mouse Imaging System (2023)

&lt;div&gt;Abstract&lt;p&gt;With the use of dual-color fluorescent cells and a highly sensitive whole-mouse imaging system with both macro-optics and micro-optics, we report here the development of subcellular real-time imaging of cancer cell trafficking in live mice. To observe cytoplasmic and nuclear dynamics in the living mouse, tumor cells were labeled in the nucleus with green fluorescent protein and with red fluorescent protein in the cytoplasm. Dual-color cancer cells were injected by a vascular route in an abdominal skin flap in nude mice. The mice were imaged with an Olympus OV100 whole-mouse imaging system with a sensitive CCD camera and five objective lenses, parcentered and parfocal, enabling imaging from macrocellular to subcellular. We observed the nuclear and cytoplasmic behavior of cancer cells in real time in blood vessels as they moved by various means or adhered to the vessel surface in the abdominal skin flap. During extravasation, real-time dual-color imaging showed that cytoplasmic processes of the cancer cells exited the vessels first, with nuclei following along the cytoplasmic projections. Both cytoplasm and nuclei underwent deformation during extravasation. Different cancer cell lines seemed to strongly vary in their ability to extravasate. With the dual-color cancer cells and the highly sensitive whole-mouse imaging system described here, the subcellular dynamics of cancer metastasis can now be observed in live mice in real time. This imaging technology will enable further understanding of the critical steps of metastasis and provide visible targets for antimetastasis drug development. (Cancer Res 2006; 66(8): 4208-14)&lt;/p&gt;&lt;/div&gt;
Figure legends text file from Therapeutic Implications of p53 Status on Cancer Cell Fate Following Exposure to Ionizing Radiation and the DNA-PK Inhibitor M3814 (2023)

&lt;p&gt;Text file with figure legends and two references cited in Fig S6 legend&lt;/p&gt;