gebhart/patrepeval huggingface dataset:
Functionally Graded Cathode Catalyst Layers for Polymer Electrolyte Fuel Cells (2004)

The effect of Nafion loading on the electrode polarization characteristics of a proton exchange membrane fuel cell is studied with a macrohomogeneous model. The composition dependence of performance is rationalized by first relating mass fractions of the different components to their volume fractions and thereafter involving concepts of percolation theory to parameterize effective properties of the cathode catalyst layers. In particular, we explore systematically the effect of Nafion content on the performance. For a uniform layer, the best performance is obtained with a Nafion content of about 35 wt %, representing an optimum balance of proton transport, oxygen diffusion, and electrochemically active surface area. With the help of this modeling tool, we propose a nonuniform Nafion catalyst layer and the modeling indicates that such a layer improves performance. Our preliminary experiments (to appear in Part II) confirm this claim. The two cases of nonuniform Nafion distribution across the entire thickness include: a three-sublayer structure with equally thick layers, simulating a constant gradient, and a two-sublayer structure with variable thickness of the sublayers. Compared with the optimum Nafion content (35 wt %) in uniform distribution, the three-sublayer structure with higher Nafion content on the membrane side exhibits significantly enhanced performance. © 2004 The Electrochemical Society. All rights reserved.
Automated Assembly of Electronic Digital Materials (2016)

Interest in additive manufacturing has recently been spurred by the promise of multi-material printing and the ability to embed functionality and intelligence into objects. Here, we present an alternative to additive manufacturing, introducing an end-to-end workflow in which discrete building blocks are reversibly joined to produce assemblies called digital materials. We describe the design of the bulk-material building blocks and the devices that are assembled from them. Further, we detail the design and implementation of an automated assembler, which takes advantage of the digital material structure to restore positioning errors within a large tolerance. To generate assembly sequences, we use a novel CAD/CAM workflow for designing, simulating, and assembling digital materials. Finally, we evaluate the structures assembled using this process, showing that the joints perform well under varying conditions and that the assembled structures are functionally precise.
Merging hypervalent iodine and sulfoximine chemistry: a new electrophilic trifluoromethylation reagent (2019)

Electrophilic trifluoromethylation is at the forefront of methodologies available for the installation of the CF<sub>3</sub> moiety to organic molecules; research in this field is largely spurred by the availability of stable and accessible trifluoromethylation reagents, of which hypervalent iodine and sulfoximine based compounds have emerged as two prominent reagent classes. Herein, we describe the facile synthesis of an electrophilic trifluoromethylation reagent which merges these two scaffolds in a novel hypervalent iodosulfoximine compound. This presents the first analogue of the well-known Togni reagents which neither compromises stability or reactivity. The electronic and physical properties of this new compound were fully explored by X-ray crystallography, cyclic voltammetry, TGA/DSC and DFT analysis. This solution stable, crystalline reagent was found to be competent in the electrophilic trifluoromethylation of a variety of nucleophiles as well as a source of the trifluoromethyl radical. Furthermore, the possibility of enantioinductive transformations could be probed with the isolation of the first enantiopure hypervalent iodine compound bearing a CF<sub>3</sub> group, thus this new reagent scaffold offers the opportunity of structurally diversifying the reagent towards asymmetric synthesis.
5 Predictions for Prenatal Genetic Testing in 2015 (2015)

These 5 predictions by Adam Wolfberg, MD, provide insight into how genetic testing—NIPT to microarray and cost to quality—continues to evolve.
Performance Analysis of OFDM Systems with Adaptive Sub Carrier Bandwidth (2008)

Orthogonal frequency division multiplexing based wireless systems are spectrally efficient, but they are vulnerable to inter-carrier interference (ICI). In a wide area scenario, users will experience varying signal strength due to different individual path loss and also varying amount of Doppler spread because of their independent velocities. Therefore, the ICI among users will vary over a wide range. It is proposed in this work to use dynamically adaptive sub carrier bandwidth (ASB) along with adaptive bit loading to mitigate ICI in such conditions, which will keep receivers simple while maintaining maximum throughput in each situation. Results show that ASB can provide higher throughput than its fixed sub carrier bandwidth counterpart when both may use adaptive bit loading per sub carrier.
An optoelectronic adaptive resonance unit (2002)

The authors demonstrate a hardware implementation of the adaptive resonance theory ART 1 neural network architecture. The optoelectronic ART1 unit, is a novel application of an old device. This device-the 4-f or Van der Lugt correlator-has historically been used as a fast pattern classifier. Usually the correlation operation is employed as a matched filter, so that a maximum correlation peak corresponds to a well-matched pattern. The device described also uses the large peaks, but takes specific advantage of the fact that a zero-shift correlation is mathematically equivalent to a two-dimensional inner product. The authors describe a promising method for emulating an ART1 unit in optics. They review ART1 from an algorithmic point of view, which shows that inner products are a critical part of ART1. They then discuss its implementation, and show some experimental results. The device works by performing the most computationally intensive parts of the algorithm in optical hardware, and thus offers a suitable marriage of the strengths of electronics and optics.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
An Easy Synthesis of Monofluorinated Derivatives of Pyrroles from β-Fluoro-β-Nitrostyrenes (2021)

The catalyst-free conjugate addition of pyrroles to β-Fluoro-β-nitrostyrenes was investigated. The reaction was found to proceed under solvent-free conditions to form 2-(2-Fluoro-2-nitro-1-arylethyl)-1<i>H</i>-pyrroles. The effectiveness of this approach was demonstrated through the preparation of a series of the target products in a quantitative yield. The kinetics of a conjugate addition of pyrrole was studied in detail to reveal the substituent effect and activation parameters of the reaction. The subsequent base-induced elimination of nitrous acid afforded a series of novel 2-(2-Fluoro-1-arylvinyl)-1<i>H</i>-pyrroles prepared in up to an 85% isolated yield. The two-step sequence herein proposed is an indispensable alternative to a direct reaction with elusive and unstable 1-Fluoroacetylenes.
Differentiation of Neuroepithelia from Human Embryonic Stem Cells (2009)

We describe the method for efficiently differentiating human embryonic stem cells to neuroepithelial cells in a chemically defined condition. The protocol was established based on the fundamental principle of in vivo neuroectodermal development. The temporal course, morphological transformation, and shift in gene expression of our neuroepithelial differentiation closely resemble those occur during in vivo development. In particular, the primitive neuroepithelial cells generated by this protocol can be further induced into neuronal and glial cells with forebrain, mid/hind brain, and spinal cord identities and targeted transmitter phenotypes.
The Role of Single-Nucleotide Polymorphisms in Pituitary Adenomas Tumorigenesis (2019)

Pituitary adenomas (PAs) are among the most common intracranial neoplasms, but despite their histologically benign nature, these tumors sometimes grow large enough to cause symptoms of mass effect such as vision loss, headaches, or hypopituitarism. When they get this large, surgery will unfortunately not be curative and, other than prolactinomas, medical options are limited, and radiation has variable efficacy in controlling growth. Understanding the genetic perturbations, such as single nucleotide polymorphisms (SNPs), that promote the formation or growth of functional and nonfunctional PAs is important because such genetic insights could improve the diagnosis and subsequent classification of PAs as well as unlock potential therapeutic targets outside contemporary standard of care. While there have been great strides in the research of SNPs as drivers of PA formation and maintenance, a comprehensive discussion of these genetic mutations has not been undertaken. In the present article, and with the goal of providing scientists and clinicians a central review, we sought to summarize the current literature on SNPs and their relationship to PA formation. Across multiple tumor types, such as nonfunctioning PAs, prolactinomas, corticotroph adenomas, somatotroph adenomas, thyrotropic adenomas, and gonadotroph adenomas, SNPs in cell surface receptors implicated in proliferation can be appreciated. Polymorphisms found in tumor suppressors and cell cycle regulators have also been identified, such as p53 SNPs in nonfunctioning PAs or cyclin D1 in prolactinomas. While the translational relevance of SNPs in the formation of PAs is still in the early stages, the use of wide-scale genomic analysis to identify patients at risk for developing PAs could yield therapeutic benefit in the future.
High-Throughput Image-Based Aggresome Quantification (2020)

Aggresomes are subcellular perinuclear structures where misfolded proteins accumulate by retrograde transport on microtubules. Different methods are available to monitor aggresome formation, but they are often laborious, time-consuming, and not quantitative. Proteostat is a red fluorescent molecular rotor dye, which becomes brightly fluorescent when it binds to protein aggregates. As this reagent was previously validated to detect aggresomes, we have miniaturized its use in 384-well plates and developed a method for high-throughput imaging and quantification of aggresomes. Two different image analysis methods, including one with machine learning, were evaluated. They lead to similar robust data to quantify cells having aggresome, with satisfactory Z' factor values and reproducible EC<sub>50</sub> values for compounds known to induce aggresome formation, like proteasome inhibitors. We demonstrated the relevance of this phenotypic assay by screening a chemical library of 1280 compounds to find aggresome modulators. We obtained hits that present similarities in their structural and physicochemical properties. Interestingly, some of them were previously described to modulate autophagy, which could explain their effect on aggresome structures. In summary, we have optimized and validated the Proteostat detection reagent to easily measure aggresome formation in a miniaturized, automated, quantitative, and high-content assay. This assay can be used at low, middle, or high throughput to quantify changes in aggresome formation that could help in the understanding of chemical compound activity in pathologies such as protein misfolding disorders or cancer.
Broadband mid‐infrared emission from Cr 2+ in crystal‐in‐glass composite glasses by Hot Uniaxial Pressing (2019)

Abstract Cr 2+ ‐doped II‐VI crystals have witnessed an excellent gain media for continuously tunable and femtosecond‐pulsed lasers. Despite this, major challenges persist toward realizing ultrabroad emission bandwidth and efficient Cr 2+ ‐doped fiber due to the valence diversity of Cr, especially in chalcogenide glasses. Here, we propose to prepare Cr 2+ :ZnSe/As 2 S 3‐x Se x composite glasses by Hot Uniaxial Pressing (HUP), a method that sinters uniformly mixed crystal and glass powders into geometrically designed composite chalcogenide glasses. The densification of the composite glasses reached 99.88%, indicating that a few or none pores remain. Our research shows that Cr 2+ :ZnSe crystals have good performance in chalcogenide glasses, and the composite glasses have the potential to be made into mid‐infrared–doped fibers. It was demonstrated by scanning electron microscopy (SEM) and X‐ray diffraction (XRD) that the composite glasses have a uniform Cr 2+ :ZnSe distribution and no crystal disintegration. The transmittance of the composite glasses was significantly improved by tailoring the refraction index. The mid‐infrared (MIR) fluorescence and decay of the glasses were measured. The lattice constant was measured, calculated, and discussed to reveal the influence of sintering process on lifetime.
Streamlined and sensitive mono- and diribosome profiling in yeast and human cells (2023)

Abstract Ribosome profiling has unveiled diverse regulations and perturbations of translation through a transcriptome-wide survey of ribosome occupancy, read out by sequencing of ribosome-protected mRNA fragments. Generation of ribosome footprints and their conversion into sequencing libraries is technically demanding and sensitive to biases that distort the representation of physiological ribosome occupancy. We address these challenges by producing ribosome footprints with P1 nuclease rather than RNase I and replacing RNA ligation with Ordered Two-Template Relay, a single-tube protocol for sequencing library preparation that incorporates adapters by reverse transcription. Our streamlined approach reduced sequence bias and enhanced enrichment of ribosome footprints relative to ribosomal RNA. Furthermore, P1 nuclease preserved a myriad of distinct juxtaposed ribosome complexes informative about yeast and human ribosome fates during translation initiation, stalling, and termination. Our optimized methods for mRNA footprint generation and capture provides a richer translatome profile using lower input and fewer technical challenges.
Criminal justice processing of sexual assault cases. Highlights. (1994)

This article discusses the processing of criminal justice on sexual assault cases in Canada. To begin with, in 1983, Bill C-127 abolished the offense of rape and indecent assault and created three new crimes of sexual assault and three parallel offenses of assault. This legislation also introduced a number of important changes to the way crimes of sexual aggression are processed by the criminal justice system. In 1991, the Supreme Court struck down provisions of the sexual assault legislation preventing a defendant from introducing evidence regarding complainant's previous sexual conduct. As a result, Bill C-49 was introduced to provide a test to determine whether a complainant's sexual history could be admitted at trial. This bill also addresses the issue of consent and the defense of mistaken beliefs in consent. The focus of the Juristat is the criminal justice processing of the three levels of sexual assault, which are elaborated in this article. In order to distinguish between the different levels, body harm relates only to physical injury and does not include psychological harm. Drawing on the Uniform Crime Reporting Survey, the Sentence Study, the Adult Criminal Court Survey and the Youth Court Survey, the Juristat summarizes recent trends relating to the processing of sexual assault and assault by the police and the courts. Canada's Violence Against Women Survey provides a profile of sexual assault incidents among adult women in Canada.
Effect of lignin on the material properties of chemically modified fibres by periodate oxidation and borohydride reduction (2016)

Effect of lignin on the material properties of chemically modified fibres by periodate oxidation and borohydride reduction
Cytotoxic Diterpenoids and Sesquiterpenoids from Pteris multifida (2008)

Three new ent-kaurane diterpenoids, named pterokaurane M 1-M 3 ( 1- 3) and three new C 14 pterosin-sesquiterpenoids, named multifidoside A-C ( 4- 6), along with 18 known compounds, were isolated from the whole plants of Pteris multifida . The structures of 1- 6 were established using spectroscopic methods, including extensive 2D NMR and CD analyses. Compounds 4 and 5 showed cytotoxicity against the HepG2 tumor cell line with IC 50 values of less than 10 microM.
A high performance Micro Channel interface for real-time industrial image processing applications (2002)

Data collection and transfer devices are critical to the performance of any machine vision system. The interface described in this paper collects image data from a color line scan camera and transfers the data obtained into the system memory of a Micro Channel-based host computer. A maximum data transfer rate of 20 Mbytes/sec can be achieved using the DMA capabilities of the camera interface. Programmability of the interface provides flexibility in selection of features such as collected field-of-view, data format, collection method, and camera control. System design is such that the speed of the interface allows for the collection of images intended for real-time processing by any available Micro Channel resident processor(s).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Clinical pharmacology of lumiracoxib, a second-generation cyclooxygenase 2 selective inhibitor (2005)

Although highly selective cyclooxygenase (COX)-2 inhibitors have been shown to be less toxic to the gastrointestinal tract than conventional non-steroidal anti-inflammatory drugs (NSAIDs), their overall safety profile is questioned. Since different selective COX-2 inhibitors were found to be associated with increased cardiovascular thrombotic events, the thrombotic hazard may be a class effect. Furthermore, warnings have been issued regarding serious skin and hypersensitivity reactions associated with valdecoxib. Lumiracoxib is a novel COX-2 selective inhibitor (coxib) with improved biochemical selectivity over that of currently available coxibs. It is structurally distinct from other drugs in the class and has weakly acidic properties. Clinical studies support a once-daily dosing regimen, despite its relatively short plasma elimination half-life (3 - 6 h). In randomised, controlled clinical trials, lumiracoxib 100 - 200 mg/day has been shown to be superior to placebo in patients with symptomatic osteoarthritis, with clinical efficacy similar to diclofenac 150 mg/day, celecoxib 200 mg/day or rofecoxib 25 mg/day. Furthermore, lumiracoxib 200 - 400 mg/day appeared to be effective in patients with rheumatoid arthritis. In patients with acute pain related to primary dysmenorrhoea, dental or orthopaedic surgery, lumiracoxib 400 mg/day was found to be at least as effective as standard doses of traditional NSAIDs and other coxibs. Endoscopic studies have indicated that lumiracoxib is associated with a rate of gastroduodenal ulcer formation that is significantly lower than with ibuprofen and does not differ from celecoxib. In the Therapeutic Arthritis Research and Gastrointestinal Trial, which enrolled 18,325 patients with osteoarthritis, the cumulative 1-year incidence of ulcer complications (primary end point) was significantly reduced by approximately threefold on lumiracoxib 400 mg/day compared with naproxen 1000 mg/day or ibuprofen 2400 mg/day (0.32 versus 0.91%). Reduction in ulcer complications was more pronounced in the population not taking low-dose aspirin (0.2 versus 0.92%, respectively). Conversely, the gastrointestinal advantage of lumiracoxib was abrogated in patients receiving low-dose aspirin (0.69 versus 0.88%, respectively, p = 0.49). Regarding cardiovascular events contributing to the trialists' composite end point (myocardial infarction, stroke or cardiovascular death), there was no significant difference between lumiracoxib (0.65%) versus combined comparator NSAIDs (0.55%). Similarly, no significant difference was recorded in rates of myocardial infarction (clinical and silent) between the lumiracoxib (0.25%) and the combined NSAID (0.19%) treatment groups. Liver function test abnormalities were more frequent with lumiracoxib (2.57%) than with the comparator NSAIDs (0.63%). Whether or not this would result in an increased risk of clinical hepatitis in the real world setting is unforeseeable.
Assessment of outcome prediction models for patients with localized prostate carcinoma managed with radical prostatectomy or external beam radiation therapy (1998)

proposed clinical staging systems to predict time to prostate specific antigen (PSA) failure after radical prostatectomy or external beam radiation therapy for clinically
Automated deep brain stimulation programming with safety constraints for tremor suppression in patients with Parkinson’s disease and essential tremor (2022)

<i>Objective.</i>Deep brain stimulation (DBS) programming for movement disorders requires systematic fine tuning of stimulation parameters to ameliorate tremor and other symptoms while avoiding side effects. DBS programming can be a time-consuming process and requires clinical expertise to assess response to DBS to optimize therapy for each patient. In this study, we describe and evaluate an automated, closed-loop, and patient-specific framework for DBS programming that measures tremor using a smartwatch and automatically changes DBS parameters based on the recommendations from a closed-loop optimization algorithm thus eliminating the need for an expert clinician.<i>Approach.</i>Bayesian optimization which is a sample-efficient global optimization method was used as the core of this DBS programming framework to adaptively learn each patient's response to DBS and suggest the next best settings to be evaluated. Input from a clinician was used initially to define a maximum safe amplitude, but we also implemented 'safe Bayesian optimization' to automatically discover tolerable exploration boundaries.<i>Main results.</i>We tested the system in 15 patients (nine with Parkinson's disease and six with essential tremor). Tremor suppression at best automated settings was statistically comparable to previously established clinical settings. The optimization algorithm converged after testing15.1±0.7settings when maximum safe exploration boundaries were predefined, and17.7±4.9when the algorithm itself determined safe exploration boundaries.<i>Significance.</i>We demonstrate that fully automated DBS programming framework for treatment of tremor is efficient and safe while providing outcomes comparable to that achieved by expert clinicians.