gebhart/patrepeval huggingface dataset:
Rational Fabrication of Nano-to-Microsphere Polycrystalline Opals Using Slope Self-Assembly (2021)

Self-assembly of artificial opals has garnered significant interest as a facile nanofabrication technique capable of producing highly ordered structures for optical, electrochemical, biomolecular, and thermal applications. In these applications, the optimum opal particle diameter can vary by several orders of magnitude because the properties of the resultant structures depend strongly on the feature size. However, current opal fabrication techniques only produce high-quality structures over a limited range of sphere sizes or require complex processes and equipment. In this work, the rational and simple fabrication of polycrystalline opals with diameters between 500 nm and 10 μm was demonstrated using slope self-assembly of colloids suspended in ethanol–water. The role of the various process parameters was elucidated through a scaling-based model that accurately captures the variations of opal substrate coverage for spheres of size 2 μm or smaller. For spheres of 10 μm and larger, capillary forces were shown to play a key role in the process dynamics. Based on these insights, millimeter-scale monolayered opals were successfully fabricated, while centimeter-scale opals were possible with sparse sphere stacking or small uncovered areas. These insights provide a guide for the simple and fast fabrication of opals that can be used as optical coatings, templates for high power density electrodes, molecule templates, and high-performance thermo-fluidic devices.
EFFECT OF GRANULOCYTE COLONY-STIMULATING FACTOR ON SYSTEMIC AND PULMONARY RESPONSES TO ENDOTOXIN IN PIGS (1993)

Granulocyte colony-stimulating factor (G-CSF) stimulates the production and function of neutrophils (PMNs). Administration of G-CSF to non-neutropenic animals has been shown to improve survival in experimental models of infection, but PMNs have been implicated as mediators of acute lung injury induced by lipopolysaccharide (LPS) or bacteremia. Thus G-CSF-induced neutrophilia might be deleterious in sepsis. To investigate this possibility, we studied four groups of pigs: G+E50 (n = 6) were pretreated for 5 days with recombinant bovine (rb) G-CSF (5 micrograms/kg/day) and then challenged with LPS (50 micrograms/kg); NS+E50 (n = 6) were similarly pretreated with saline and challenged with LPS (50 micrograms/kg); E250 (n = 6) were not pretreated and were infused with a larger dose of LPS (250 micrograms/kg); RL (n = 7) were controls infused with lactated Ringer's solution. Pretreatment with rbG-CSF increased the peripheral absolute neutrophil count approximately fivefold (p < 0.05 vs. RL group). Comparisons of the NS+E50 and G+E50 groups showed that pretreatment with rhG-CSF did not affect LPS-induced alterations in mean arterial blood pressure or arterial oxygenation. Indices of pulmonary injury also were similar in these two groups, although pulmonary edema and protein leakage into alveoli were greater in the E250 group. We conclude that G-CSF-induced neutrophilia does not adversely effect physiologic responses to LPS in pigs.
Use of neural networks in ground-based aerosol retrievals from multi-angle spectropolarimetric observations (2015)

Abstract. In this paper, the use of a neural network algorithm for the retrieval of the aerosol properties from ground-based spectropolarimetric measurements is discussed. The neural network is able to retrieve the aerosol properties with an accuracy that is almost comparable to that of an iterative retrieval. By using the outcome of the neural network as first guess in the iterative retrieval scheme, the accuracy of the retrieved fine- and coarse-mode optical thickness is further improved, while for the other parameters the improvement is small or absent. The resulting scheme (neural network + iterative retrieval) is compared to the original one (look-up table + iterative retrieval) on a set of simulated ground-based measurements, and on a small set of real observations carried out by an accurate ground-based spectropolarimeter. The results show that the use of a neural-network-based first guess leads to an increase in the number of converging retrievals, and possibly to more accurate estimates of the aerosol effective radius and complex refractive index.
Smart antenna technologies for future wireless systems: trends and challenges (2004)

The adaptation of smart antenna techniques in future wireless systems is expected to have a significant impact on the efficient use of the spectrum, the minimization of the cost of establishing new wireless networks, the optimization of service quality, and realization of the transparent operation across multitechnology wireless networks. Nevertheless, its success relies on two considerations that have been often overlooked when investigating smart antenna technologies: first, the smart antennas features need to be considered early in the design phase of future systems (top-down compatibility); second, a realistic performance evaluation of smart antenna technique needs to be performed according to the critical parameters associated with future systems requirements (bottom-up feasibility). In this article an overview of the benefits of and most recent advances in smart antenna transceiver architecture is given first. Then the most important trends in the adoption of smart antennas in future system are presented, such as reconfigurability to varying channel propagation and network conditions, cross-layer optimization, and multi-user diversity, as well as challenges such as the design of a suitable simulation methodology and the accurate modeling of channel characteristics, interference, and implementation losses. Finally, market trends, future projections, and the expected financial impact of smart antenna systems deployment are discussed.
Evidence from coadsorption studies for a molecular precursor state in the oxidation of Zn(0001) (1988)

The rate of dissociative chemisorption of dioxygen at a Zn(0001) surface at 110 K increases by a factor of nearly 103 when coadsorbed with ammonia; a model based on a precursor state involving an ammonia–dioxygen complex is suggested.
ChemInform Abstract: Ozonolysis of Olefinic Phenyl Selenides with Preservation of the Selenium Unit: A Route to Phenylseleno Aldehydes and Ketones. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Synthesis and structure-activity relationships of some 6.BETA.-acrylamido penicillins. (1993)

Syntheses are described for penicillins (4b approximately 4i, 5a and 5b) which possess a 6 beta-(2-heteroaryl-3-substituted)-propenamido side-chain of fixed geometry. In vitro results for these compounds against a range of Gram-positive and Gram-negative bacteria showed in most cases good stability against both penicillinase and TEM-1 beta-lactamase; analogues (4b approximately 4i) bearing a 2-(2-aminothiazol-4-yl) unit showed the best intrinsic activity, the cyclohexyl compound (4b) being the most promising. The 1-acetoxyethyl ester (6) of 4b was also prepared; in experimental animal studies the in vivo properties of this compound compared favourably with cefuroxime axetil and are reported together with selected in vivo data for the other compounds.
BIST-based test and diagnosis of FPGA logic blocks (2001)

We present a built-in self-test (BIST) approach able to detect and accurately diagnose all single and practically all multiple faulty programmable logic blocks (PLBs) in field programmable gate arrays (FPGAs) with maximum diagnostic resolution. Unlike conventional BIST, FPGA BIST does not involve any area overhead or performance degradation. We also identify and solve the problem of testing configuration multiplexers that was either ignored or incorrectly solved in most previous work. We introduce the first diagnosis method for multiple faulty PLBs; for any faulty PLB, we also identify its internal faulty modules or modes of operation. Our accurate diagnosis provides the basis for both failure analysis used for yield improvement and for any repair strategy used for fault-tolerance in reconfigurable systems. We present experimental results showing detection and identification of faulty PLBs in actual defective FPGAs. Our BIST architecture is easily scalable.
Material contrast based inline metrology: process verification and control using back scattered electron imaging on CD-SEM (2013)

The Critical Dimension Scanning Electron Microscope (CDSEM) is the traditional workhorse solution for inline process control. Measurements are extracted from top-down images based on secondary electron collection while scanning the specimen. Secondary electrons holding majority of detection yield. These images provide more on the structural information of the specimen surface and less in terms of material contrast. In some cases there is too much structural information in the image which can irritate the measurement, in other cases small but important differences between various material compounds cannot be detected as images are limited by contrast information and resolution of primary scanning beam. Furthermore, accuracy in secondary electron based metrology is limited by charging. To gather the exact required information for certain material compound as needed, a technique, known from material analytic SEM´s has been introduced for inline CDSEM analysis and process control: Low Loss Back Scattered Electron Imaging (LL-BSE). The key at LL-BSE imaging is the collection of only the back scattered electrons (BSE) from outermost specimen surface which undergo the least amount possible of energy loss in the process of image generation following impact of the material by a primary beam. In LL-BSE very good and measurable material distinction and sensitivity, even for very low density material compounds can be achieved. This paper presents new methods for faster process development cycle, at reduced cost, based on LL-BSE mass data mining instead of sending wafers for destructive material analysis.
Long-Range Structural Restraints in Spin-Labeled Proteins Probed by Solid-State Nuclear Magnetic Resonance Spectroscopy (2007)

Magic-angle spinning NMR studies of 13C,15N-enriched proteins containing a covalently attached nitroxide spin label are presented, with the goal of using paramagnetic relaxation enhancements in the solid state to obtain long-range structural restraints. A 56 aa protein, B1 immunoglobulin-binding domain of protein G (GB1), was used as a model system, with nitroxide spin labels incorporated at residues 28 (α-helix) or 53 (β4-strand) via site-directed spin labeling. The presence of nitroxide spin labels in the GB1 analogues results in negligible pseudocontact shifts and large enhancement of the transverse relaxation rates for a number of residues, as detected by 2D 15N-13Cα correlation spectroscopy. The experimentally observed relaxation rate enhancements are found to be highly correlated with the distance of 1H, 15N, and 13Cα nuclei from the electron spin, with significant relaxation effects observed for nuclei up to ∼20 Å away, thus providing valuable information about the protein fold on length scales inaccessible to traditional solid-state NMR techniques.
Supplementary Figures 1-3 from Activation of the PI3K/Akt/mTOR and MAPK Signaling Pathways in Response to Acute Solar-Simulated Light Exposure of Human Skin (2023)

&lt;p&gt;Supplementary Figure 1. Box plot graphs of protein expression in sun-protected skin after 2, 2,5, and 3 MED (x-axis) of SSL-irradiation (SSL) with baseline (green bars), 5 minutes (orange bars), 1 hour (blue bars), 5 hours (red bars), and 24 hours (grey bars) post SSL on the y-axis. Supplementary Figure 2. Box plot graphs of protein expression in sun-protected skin after 2, 2,5, and 3 MED (x-axis) of SSL-irradiation (SSL) with baseline (green bars), 5 minutes (orange bars), 1 hour (blue bars), 5 hours (red bars), and 24 hours (grey bars) post SSL on the y-axis. Supplementary Figure 3. Box plot graphs of protein expression in sun-protected skin after 2, 2,5, and 3 MED (x-axis) of SSL-irradiation (SSL) with baseline (green bars), 5 minutes (orange bars), 1 hour (blue bars), 5 hours (red bars), and 24 hours (grey bars) post SSL on the y-axis.&lt;/p&gt;
The SOX9 upstream region prone to chromosomal aberrations causing campomelic dysplasia contains multiple cartilage enhancers (2015)

Two decades after the discovery that heterozygous mutations within and around SOX9 cause campomelic dysplasia, a generalized skeleton malformation syndrome, it is well established that SOX9 is a master transcription factor in chondrocytes. In contrast, the mechanisms whereby translocations in the --350/-50-kb region 5' of SOX9 cause severe disease and whereby SOX9 expression is specified in chondrocytes remain scarcely known. We here screen this upstream region and uncover multiple enhancers that activate Sox9-promoter transgenes in the SOX9 expression domain. Three of them are primarily active in chondrocytes. E250 (located at -250 kb) confines its activity to condensed prechondrocytes, E195 mainly targets proliferating chondrocytes, and E84 is potent in all differentiated chondrocytes. E84 and E195 synergize with E70, previously shown to be active in most Sox9-expressing somatic tissues, including cartilage. While SOX9 protein powerfully activates E70, it does not control E250. It requires its SOX5/SOX6 chondrogenic partners to robustly activate E195 and additional factors to activate E84. Altogether, these results indicate that SOX9 expression in chondrocytes relies on widely spread transcriptional modules whose synergistic and overlapping activities are driven by SOX9, SOX5/SOX6 and other factors. They help elucidate mechanisms underlying campomelic dysplasia and will likely help uncover other disease mechanisms.
FUTURE PERSPECTIVES IN MELANOMA RESEARCH. Meeting report from the "Melanoma Research: a bridge from Naples to the World. Napoli, (2012)

After more than 30 years, landmark progress has been made in the treatment of cancer, and melanoma in particular, with the success of new molecules such as ipilimumab, vemurafenib and active specific immunization. After the first congress in December 2010, the second edition of “Melanoma Research: a bridge from Naples to the World” meeting, organized by Paolo A. Ascierto (INT, Naples, Italy), Francesco M. Marincola (NIH, Bethesda, USA), and Nicola Mozzillo (INT, Naples, Italy) took place in Naples, on 5–6 December 2011. We have identified four new topics of discussion: Innovative Approaches in Prevention, Diagnosis and Surgical Treatment, New Pathways and Targets in Melanoma: An Update about Immunotherapy, and Combination Strategies. This international congress gathered more than 30 international faculty members and was focused on recent advances in melanoma molecular biology, immunology and therapy, and created an interactive atmosphere which stimulated discussion of new approaches and strategies in the field of melanoma.
Comparative evaluation of laser-assisted micro-milling for AISI 316, AISI 422, TI-6AL-4V and Inconel 718 in a side-cutting configuration (2010)

This paper is focused on numerical modeling and experimental evaluation of laser-assisted micro-milling (LAMM). An experimental setup consisting of a 25 W CO2 laser, three-axis CNC linear stages and a high-speed spindle was used to implement the LAMM process. Micro-endmills between 100 and 300 µm in diameter were used to perform side-cutting operations with and without laser preheat on four materials: AISI 316, AISI 422, Ti-6Al-4V and Inconel 718. A three-dimensional transient finite-volume-based thermal model was used to analytically predict appropriate process parameters on the basis of material-removal temperatures. The effects of LAMM on the machined surface finish, edge burrs, tool wear and workpiece microstructure were evaluated experimentally.
Reconfigurable Photonic Crystal Circuits and Fibers Using Microfluidics (2009)

Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text B. J. Eggleton, "Reconfigurable Photonic Crystal Circuits and Fibers Using Microfluidics," in Conference on Lasers and Electro-Optics/International Quantum Electronics Conference, OSA Technical Digest (CD) (Optica Publishing Group, 2009), paper CTuD1. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Electrochemical Oxidation of a Highly Soluble Redox Mediator in Aqueous Solution for Energy Conversion (2019)

An efficient and cost-effective electrochemical energy storage/conversion technique is needed to store electricity from intermittent renewable energy sources. Although electrochemical water splitting into hydrogen and oxygen is extensively studied, it suffers from kinetically sluggish oxygen evolution reactions at extreme pH conditions. In this work, we investigated the oxidation of soluble redox species as an alternative anodic reaction, the kinetics of which have highly reversible characteristics on various electrode surfaces. The redox potential can be readily tuned by the ligand upon the formation of metal complexes, which provides various voltage options to develop electrochemical devices. The facile kinetics lead to diffusion overpotential being a major contribution of the electrochemical performance. Near-neutral pH region was effectively chosen because it allows the versatile and safe operation with a reasonable voltage option. To overcome the diffusion issue, the solubility of redox species and the diffusion coefficient determined by temperature and the supporting buffer were considered. Fe2+/3+–(hydroxyethylethylenediaminetriacetic acid) (HEDTA) was identified as a suitable redox candidate due to its high solubility (>1 mol kg–1) in a near-neutral pH range from 4 to 6. In the optimized conditions with a malonate buffer, the diffusion-limiting current density could reach over 100 mA cm–2 on a glassy carbon rotating disk electrode. The detailed insight presented in this paper will help to design an efficient electrochemical system for redox-mediated energy storage/conversion.
Young SHR express increased type 1 angiotensin II receptors in renal proximal tubule (1998)

A potential role for the renin-angiotensin system (RAS) in the development and/or maintenance of hypertension in the genetic model of rat hypertension, spontaneously hypertensive rats (SHR), has been suggested by studies indicating that treatment of immature animals with angiotensin-converting enzyme (ACE) inhibitors prevents subsequent development of hypertension. Because young SHR also demonstrate RAS-dependent increased sodium retention, we examined proximal tubule type 1 angiotensin II receptor (AT1R) mRNA expression in young (4 wk) or adult (14 wk) SHR compared with age-matched Wistar-Kyoto (WKY) rats. Proximal tubules were isolated by Percoll gradient centrifugation, and AT1R mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction (RT-PCR). At 14 wk, when SHR had established hypertension [mean arterial blood pressure (MAP) of SHR vs. WKY: 145 +/- 6 vs. 85 +/- 5 mmHg, n = 14-15], there were no differences in proximal tubule AT1R mRNA levels [SHR vs. WKY: 79 +/- 14 vs. 72 +/- 14 counts/min (cpm) per cpm mutant AT1R per cpm beta-actin x 10(-6), n = 6; not significant (NS)]. In contrast, in 4 wk SHR, at a time of minimal elevations in blood pressure (MAP: 70 +/- 8 vs. 63 +/- 3), SHR proximal tubule AT1R mRNA levels were 263 +/- 30% that of WKY (143 +/- 18 vs. 60 +/- 11 cpm per cpm of mutant AT1R per cpm beta-actin x 10(-6), n = 8; P < 0.005). We have recently shown that chronic ACE inhibition decreases proximal tubule AT1R expression and have also shown that chronic L-3,4-dihydroxyphenylalamine (L-DOPA) administration inhibits AT1R expression in adult Sprague-Dawley proximal tubule and cultured proximal tubule, and this inhibition is mediated via Gs-coupled DA1 receptors. When 3-wk-old animals were given L-DOPA or captopril for 1 wk, MAP was not altered (70 +/- 8 vs. 60 +/- 4 or 61 +/- 5 mmHg), but proximal tubule AT1R mRNA was no longer significantly different between SHR and WKY (68 +/- 9 vs. 38 +/- 7 or 20 +/- 3 vs. 47 +/- 15 cpm per cpm of mutant AT1R per cpm beta-actin x 10(-6)), due to a significant decrease in proximal tubule AT1R expression in SHR (P < 0.005, compared with untreated SHR). Immunoreactive proximal tubule AT1R expression also was increased in 4 wk SHR and was reversed with captopril or L-DOPA treatment. Therefore, these results indicate that young, but not adult, SHR have increased expression of proximal tubule AT1R and that chronic L-DOPA or captopril treatment decreased the elevated AT1R expression to control levels. These results provide further support for an important role of the RAS in the development of hypertension in SHR.
Level by level (2010)

We present a practical and scalable method for flow- and context-sensitive (FSCS) pointer analysis for C programs. Our method analyzes the pointers in a program level by level in terms of their points-to levels, allowing the points-to relations of the pointers at a particular level to be discovered based on the points-to relations of the pointers at this level and higher levels. This level-by-level strategy can enhance the scalability of the FSCS pointer analysis in two fundamental ways, by enabling (1) fast and accurate flow-sensitive analysis on full sparse SSA form using a flow-insensitive algorithm and (2) fast and accurate context-sensitive analysis using a full transfer function and a meet function for each procedure.
Cellular Imprinting Proteomics Assay: A Novel Method for Detection of Neural and Ocular Disorders Applied to Congenital Zika Virus Syndrome (2020)

Congenital Zika syndrome was first described due to increased incidence of congenital abnormalities associated with Zika virus (ZIKV) infection. Since the eye develops as part of the embryo central nervous system (CNS) structure, it becomes a specialized compartment able to display symptoms of neurodegenerative diseases and has been proposed as a noninvasive approach to the early diagnosis of neurological diseases. Ocular lesions result from defects that occurred during embryogenesis and can become apparent in newborns exposed to ZIKV. Furthermore, the absence of microcephaly cannot exclude the occurrence of ocular lesions and other CNS manifestations. Considering the need for surveillance of newborns and infants with possible congenital exposure, we developed a method termed cellular imprinting proteomic assay (CImPA) to evaluate the ocular surface proteome specific to infants exposed to ZIKV during gestation compared to nonexposure. CImPA combines surface cells and fluid capture using membrane disks and a large-scale quantitative proteomics approach, which allowed the first-time report of molecular alterations such as neutrophil degranulation, cell death signaling, ocular and neurological pathways, which are associated with ZIKV infection with and without the development of congenital Zika syndrome, CZS. Particularly, infants exposed to ZIKV during gestation and without early clinical symptoms could be detected using the CImPA method. Lastly, this methodology has broad applicability as it could be translated in the study of several neurological diseases to identify novel diagnostic biomarkers. Data are available via ProteomeXchange with identifier PXD014038.
Subclassification of desmoplastic melanoma: pure and mixed variants have significantly different capacities for lymph node metastasis (2008)

There are differences between PDM and MDM, the most important of which is the incidence of LN involvement. Our findings support the clinical utility of classifying DM into pure and mixed subtypes because the negligible rate of nodal involvement in PDM does not support the routine performance of sentinel LN biopsy in this subgroup of melanoma patients. In contrast, the incidence of LN involvement in MDM is comparable to that of CM.