gebhart/patrepeval huggingface dataset:
Ebselen protects Chinese hamster ovary cells from radiation-induced apoptosis (1997)

Radiation-induced apoptosis in chinese hamster ovary (CHO) cell lines is characterized by endonucleolytic cleavage of cellular DNA and changes of cell morphology within hours after radiation exposure. We investigated the capacity of ebselen [2-phenyl-1,2-benzisoselenazol-3(2H)-one], a seleno-organic compound with selenium-dependent glutathione peroxidase (GPx) activity, to protect cells from radiation-induced apoptosis. This phenomenon was studied by the quantitation of apoptotic cells and DNA gel electrophoresis after 6 Gy X-ray exposure. We also measured the activity of GPx and membrane lipid peroxidation. It was observed that 20 µM ebselen efficiently blocked apoptotic cell formation and DNA fragmentation 48 h post irradiation. Furthermore the data demonstrated that lipid peroxides increased significantly in irradiated cells and ebselen inhibited this process by elevating the cellular GPx activity. The results presented here indicate the requirement of free radicals for radiation-induced apoptosis and ultimately may yield insight necessary for designing protocols to modulate the process of radiation-induced apoptosis with antioxidant agents that scavenge radiation-induced free radicals.
Three-Path SiGe BiCMOS LNA on Thinned Silicon Substrate for IoT Applications (2018)

This paper reports on a design methodology and measurement results of a fully integrated low noise amplifier (LNA) on a thinned substrate for Internet of Things (IoT) applications. Several key RF performance parameters of the LNA with different substrate thickness are evaluated through full-wave electromagnetic (EM) simulations. The proposed LNA operating at 5.5 GHz is fabricated in a cost-effective 0.25 μm SiGe BiCMOS technology (IHP process SGB25V; ft = 75 GHz). The Si chip is thinned to ~38 μm in order to be embedded seamlessly into a flexible foil system. The small-signal gain of the LNA, measured on the chuck is 14.32 dB before thinning. The measured center frequency on the thin silicon (thickness of 38 μm) is shifted about 700 MHz towards higher frequencies compared to the thick silicon due to the image mirror currents within the conducting material at the backside of the chip. The measured noise figure (NF) with the thick and thin substrate on the conducting material is around 3.36 dB at 5.5 GHz and 3.74 dB at 6.3 GHz, respectively.
Characterization of a Bordetella pertussis Diaminopimelate (DAP) Biosynthesis Locus Identifies dapC , a Novel Gene Coding for an N -Succinyl- l,l -DAP Aminotransferase (2000)

The functional complementation of two Escherichia coli strains defective in the succinylase pathway of meso-diaminopimelate (meso-DAP) biosynthesis with a Bordetella pertussis gene library resulted in the isolation of a putative dap operon containing three open reading frames (ORFs). In line with the successful complementation of the E. coli dapD and dapE mutants, the deduced amino acid sequences of two ORFs revealed significant sequence similarities with the DapD and DapE proteins of E. coli and many other bacteria which exhibit tetrahydrodipicolinate succinylase and N-succinyl-L,L-DAP desuccinylase activity, respectively. The first ORF within the operon showed significant sequence similarities with transaminases and contains the characteristic pyridoxal-5'-phosphate binding motif. Enzymatic studies revealed that this ORF encodes a protein with N-succinyl-L,L-DAP aminotransferase activity converting N-succinyl-2-amino-6-ketopimelate, the product of the succinylase DapD, to N-succinyl-L,L-DAP, the substrate of the desuccinylase DapE. Therefore, this gene appears to encode the DapC protein of B. pertussis. Apart from the pyridoxal-5'-phosphate binding motif, the DapC protein does not show further amino acid sequence similarities with the only other known enzyme with N-succinyl-L,L-DAP aminotransferase activity, ArgD of E. coli.
Plasmon excitations in Josephson arrays and thin superconducting layers (1990)

Long-wave excitations in Josephson arrays are investigated. The existence of excitations with square root or acoustic dispersion law, like plasmons in two-dimensional electron gas, is predicted. Observation of such excitations with frequency much lower than the frequency of the superconducting gap \ensuremath{\Delta}/\ensuremath{\Elzxh} is possible by use of a grating and the same far infrared or microwave techniques used for detection of the plasmons in semiconductor microstructures.
Elevated Levels of Circulating lncRNAs LIPCAR and MALAT1 Predict an Unfavorable Outcome in Acute Coronary Syndrome Patients (2023)

Coronary artery disease (CAD) is a leading cause of mortality worldwide. In this study, we aimed to assess the potential of plasma long non-coding RNAs (lncRNAs) LIPCAR and MALAT1 and microRNAs (miRNAs) miR-142-3p and miR-155-5p to discriminate unstable CAD patients from stable ones. 23 stable angina (SA), 21 unstable angina (UA), and 50 ST-segment elevation myocardial infarction (STEMI) patients were enrolled; their plasma was collected. ncRNA plasma levels were evaluated using RT-qPCR. All measured ncRNA levels were significantly increased in UA patients' plasma compared to SA patients' plasma and in STEMI-with major adverse cardiovascular event (MACE) patients' plasma vs. STEMI-without MACE patients' plasma. ROC analysis showed that increased levels of LIPCAR and MALAT1 were associated with UA, and the prognostic model improved with the addition of miR-155-5p levels. The assessed lncRNAs discriminated between hyperglycemic (HG) and normoglycemic (NG) UA patients, and they were associated with MACE incidence in STEMI patients; this prediction was improved by the addition of miR-142-3p levels to the ROC multivariate model. We propose LIPCAR and MALAT1 as effective diagnostic markers for vulnerable CAD, their association with HG in UA patients, and as robust predictors for unfavorable evolution of STEMI patients.
THE FINE STRUCTURE OF THE PIGMENT EPITHELIUM IN THE ALBINO RAT (1962)

In this report, particular attention is paid to the inclusion bodies found in the apical cytoplasm of the pigment epithelial cell. These bodies are of variable size and form. The smallest (0.4 micro diameter) consist of a granular matrix enclosed by a single membrane, and are similar to the lysosomes of hepatic cells. Larger inclusion bodies contain areas of lamellated material in addition to granular matrix. The largest particles seen (2 micro diameter) are almost entirely lamellar. These different forms seem closely related, for it is possible to find all transitional stages between the smallest and largest particles. The relationship between the lamellar inclusion bodies and the rod outer segments is discussed.
Immobilized Palladium Nanoparticles Catalyzed Oxidative Carbonylation of Amines (2009)

Catalytic application of immobilized palladium nanoparticles for synthesis of ureas by oxidative carbonylation of amines has been investigated. This is the first report on oxidative carbonylation of amines to ureas using immobilized palladium nanoparticles catalyst. The palladium nanoparticles were immobilized on a NaY zeolite support through 3-aminopropyl-trimethoxysilane (APTS) as anchoring agent. The [Pd]-APTS-Y catalyst along with NaI promoter showed high conversion and selectivity to the desired urea products over several amine compounds including an example of an aliphatic amine precursor even at 333 K. The immobilized catalyst was easily separated and recycled several times without any loss of activity. The role of different solvents, iodide promoters, iodide to Pd ratio, concentration of substrates, and temperature on the overall yield of the reaction was also investigated.
Identification of the messenger RNA for human cutaneous fatty acid-binding protein as a metastasis inducer. (2000)

Using our recently developed systematic differential display and complete comparison of gene expression approaches combined with other methods, we have identified a large number of mRNAs that are expressed differentially between benign and malignant human cells. One such mRNA that is common to prostate and breast carcinoma cell lines encodes the human cutaneous fatty acid-binding protein (C-FABP). Northern and slot blot analyses confirm that the expression levels of C-FABP mRNA in the malignant prostate and breast carcinoma cell lines are 4.9+/-0.9- to 16.9+/-2.1-fold higher than those expressed in the benign cell lines. A similar difference between the benign and malignant cell lines was also detected at the protein level. In situ hybridization experiments have detected overexpression of the mRNA for C-FABP in human prostate carcinoma tissues. Transfection of a C-FABP expression construct into the benign, nonmetastatic rat mammary epithelial cell line Rama 37 and inoculation of the C-FABP expression transfectants into syngeneic Wistar-Furth rats produce a significant number (P < 0.05) of animals with metastases (6 of 26 animals), whereas the control transfectants generated by the vector alone yield no such metastases. Measurements of mRNA and protein levels with Northern and Western blotting show that C-FABP is not expressed in the control transfectant cells produced by the vector alone but is highly expressed in the pool of C-FABP transfectants and-the sublines established from their metastases. Immunocytochemical staining with antibodies to C-FABP shows that C-FABP is not expressed in the primary tumors developed from the control transfectants that have failed to metastasize, but it is expressed in both the primary tumors developed from the C-FABP transfectants and their metastases. Reinoculation of the sublines established from metastases in syngeneic rats has produced a higher proportion (50%) of animals (7 of 14 animals) with metastases than that obtained in the first-round inoculations, indicating that the metastatic clones have been preferentially selected from the original pool of metastatic and nonmetastatic transfectant clones. These results have demonstrated that elevated expression of C-FABP can induce metastasis and that metastatic capability has been transferred in a genetically dominated manner in this Rama 37 model. Thus, we suggest that C-FABP is a metastasis-inducing gene, and under suitable conditions, it may induce metastasis of some human cancers.
Positron Emission Tomography Imaging of the Gastrin-Releasing Peptide Receptor with a Novel Bombesin Analogue (2019)

The gastrin-releasing peptide receptor (GRPR), a G protein-coupled receptor, is overexpressed in solid malignancies and particularly in prostate cancer. We synthesized a novel bombesin derivative, [<sup>68</sup>Ga]Ga-ProBOMB1, evaluated its pharmacokinetics and potential to image GRPR expression with positron emission tomography (PET), and compared it with [<sup>68</sup>Ga]Ga-NeoBOMB1. ProBOMB1 (DOTA-pABzA-DIG-d-Phe-Gln-Trp-Ala-Val-Gly-His-Leu-ψ(CH<sub>2</sub>N)-Pro-NH<sub>2</sub>) was synthesized by solid-phase peptide synthesis. The polyaminocarboxylate chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) was coupled to the <i>N</i>-terminal and separated from the GRPR-targeting sequence by a <i>p</i>-aminomethylaniline-diglycolic acid (pABzA-DIG) linker. The binding affinity to GRPR was determined using a cell-based competition assay, whereas the agonist/antagonist property was determined with a calcium efflux assay. ProBOMB1 was radiolabeled with <sup>68</sup>GaCl<sub>3</sub>. PET imaging and biodistribution studies were performed in male immunocompromised mice bearing PC-3 prostate cancer xenografts. Blocking experiments were performed with coinjection of [d-Phe<sup>6</sup>,Leu-NHEt<sup>13</sup>,des-Met<sup>14</sup>]bombesin(6-14). Dosimetry calculations were performed with OLINDA software. ProBOMB1 and the nonradioactive Ga-ProBOMB were obtained in 1.1 and 67% yield, respectively. The <i>K</i> <sub>i</sub> value of Ga-ProBOMB1 for GRPR was 3.97 ± 0.76 nM. Ga-ProBOMB1 behaved as an antagonist for GRPR. [<sup>68</sup>Ga]Ga-ProBOMB1 was obtained in 48.2 ± 10.9% decay-corrected radiochemical yield with 121 ± 46.9 GBq/μmol molar activity and >95% radiochemical purity. Imaging/biodistribution studies showed that the excretion of [<sup>68</sup>Ga]Ga-ProBOMB1 was primarily through the renal pathway. At 1 h postinjection (p.i.), PC-3 tumor xenografts were clearly delineated in PET images with excellent contrast. The tumor uptake for [<sup>68</sup>Ga]Ga-ProBOMB1 was 8.17 ± 2.57 percent injected dose per gram (% ID/g) and 9.83 ± 1.48% ID/g for [<sup>68</sup>Ga]Ga-NeoBOMB1, based on biodistribution studies at 1 h p.i. This corresponded to tumor-to-blood and tumor-to-muscle uptake ratios of 20.6 ± 6.79 and 106 ± 57.7 for [<sup>68</sup>Ga]Ga-ProBOMB1 and 8.38 ± 0.78 and 39.0 ± 12.6 for [<sup>68</sup>Ga]Ga-NeoBOMB1, respectively. Blockade with [d-Phe<sup>6</sup>,Leu-NHEt<sup>13</sup>,des-Met<sup>14</sup>]bombesin(6-14) significantly reduced the average uptake of [<sup>68</sup>Ga]Ga-ProBOMB1 in tumors by 62%. The total absorbed dose was lower for [<sup>68</sup>Ga]Ga-ProBOMB1 in all organs except for bladder compared with [<sup>68</sup>Ga]Ga-NeoBOMB1. Our data suggest that [<sup>68</sup>Ga]Ga-ProBOMB1 is an excellent radiotracer for imaging GRPR expression with PET. [<sup>68</sup>Ga]Ga-ProBOMB1 achieved a similar uptake as [<sup>68</sup>Ga]Ga-NeoBOMB1 in tumors, with enhanced contrast and lower whole-body absorbed dose.
Using HDAC Inhibitors to Eradicate Adult T-Cell Leukemia-Lymphoma (2014)

Abstract Adult T-cell leukemia-lymphoma (ATLL) is an aggressive malignancy with a poor prognosis caused by HTLV-I, which is endemic in Japan, the Caribbean, and South America. ATLL cannot be cured with conventional chemotherapy thus urging the development of new therapeutic strategies. Histone deacetyalse (HDAC) inhibitors are broadly active anti-neoplastic agents, which have shown efficacy in T-cell lymphomas. At our institution we encounter a relatively high number of ATLL cases as compared to other regions in the U.S. We have conducted a pilot trial using AZT/interferon-α(IFNα) plus the HDAC inhibitor valproic acid (VPA) during maintenance therapy in acute (leukemia-type) ATLL. We hypothesized that VPA would reactivate HTLV-I leading to a cytotoxic T-cell immune response followed by reduction of residual blood circulating ATLL clones that normally persist during AZT/IFNα therapy despite its suppressive effects. Supporting this notion, the addition of VPA to AZT/IFNα resulted in reduction of HTLV-I proviral loads in treated patients and a sustained molecular response in one subject who is still alive &gt;4.5 years later. More recently, we have tested newly available and more potent HDAC inhibitors using fresh primary leukemic ATLL cells and low-passage cell lines. Through HDAC inhibition, these agents reactivated HTLV-I Tax gene expression and induced apoptosis in a dose-dependent manner. Belinostat, which is currently FDA-approved for the treatment of relapsed or refractory peripheral T-cell lymphoma, augmented ATLL cell death when added to AZT/IFNα at nanomolar concentrations. Therefore, we have proposed a new pilot clinical trial using belinostat for the treatment of ATLL during post-induction therapy. The role of HDAC inhibitors in ATLL, pre-clinical laboratory studies involving these agents, and clinical trial design will be discussed at the meeting Disclosures Off Label Use: The use of HDAC inhibitors in adult T-cell Leukemia-lymphoma.
Power System Real-Time Monitoring by Using PMU-Based Robust State Estimation Method (2015)

Accurate real-time states provided by the state estimator are critical for power system reliable operation and control. This paper proposes a novel phasor measurement unit (PMU)-based robust state estimation method (PRSEM) to real-time monitor a power system under different operation conditions. To be specific, an adaptive weight assignment function to dynamically adjust the measurement weight based on the distance of big unwanted disturbances from the PMU measurements is proposed to increase algorithm robustness. Furthermore, a statistical test-based interpolation matrix H updating judgment strategy is proposed. The processed and resynced PMU information are used as priori information and incorporated to the modified weighted least square estimation to address the measurements imperfect synchronization between supervisory control and data acquisition and PMU measurements. Finally, the innovation analysis-based bad data (BD) detection method, which can handle the smearing effect and critical measurement errors, is presented. We evaluate PRSEM by using IEEE benchmark test systems and a realistic utility system. The numerical results indicate that, in short computation time, PRSEM can effectively track the system real-time states with good robustness and can address several kinds of BD.
Pantoea:insights into a highly versatile and diverse genus within the Enterobacteriaceae (2015)

The bacterial genus Pantoea comprises many versatile species that have been isolated from a multitude of environments. Pantoea was delineated as a genus approximately 25 years ago, but since then, approximately 20 species have been identified having a diversity of characteristics. Isolates from water and soil have been harnessed for industrial purposes including bioremediation, and the degradation of herbicides and other toxic products. Other isolates possess nitrogen fixation and plant growth-promoting capabilities, which are currently being explored for agricultural applications. Some isolates are antibiotic producers, and have been developed into biocontrol agents for the management of plant diseases. Pantoea is also known to form host associations with a variety of hosts, including plants, insects and humans. Although often thought of as a plant pathogen, recent evidence suggests that Pantoea is being frequently isolated from the nosocomial environment, with considerable debate as to its role in human disease. This review will explore this highly versatile group and its capabilities, its known associations, and the underlying genetic and genomic determinants that drive its diversity and adaptability.
Novel State Estimation Method of Surface Chatter Pattern via Image Processing After End Milling (2019)

Abstract We studied a new analytical method for determining self-excited chatter vibration at an end mill processing site. When chatter vibration occurs, cutting marks with periodic features called “chatter marks” are generated on the processed surface. We focused on the relationship between the vibration generated during cutting and the roughness of the processed surface. We developed a method to analyze such vibrations, based on the roughness of the finished surface. Instead of using a roughness meter and laser-measuring instrument, which are often used for measuring the contours of a processed surface, we presumed the roughness of the processed surface from image data obtained using a digital camera. As a comparative experiment to verify the proposed method, side cutting with an end mill was performed. Chatter vibrations occurring during the cutting were measured using a displacement sensor and microphone, and compared with the vibration of the tool edge as determined from the processed surface. The chatter frequency read from the processed surface was close to that obtained from the displacement sensor and microphone. Thus, the proposed method can be used to analyze the information of chatter vibration inexpensively and easily. It can serve as a substitute for displacement and acceleration sensors.
Muscular Movement Model-Based Automatic 3D/4D Facial Expression Recognition (2016)

Facial expression is an important channel for human nonverbal communication. This paper presents a novel and effective approach to automatic 3D/4D facial expression recognition based on the muscular movement model (MMM). In contrast to most of existing methods, the MMM deals with such an issue in the viewpoint of anatomy. It first automatically segments the input 3D face (frame) by localizing the corresponding points within each muscular region of the reference using iterative closest normal point. A set of features with multiple differential quantities, including coordinate, normal, values, are then extracted to describe the geometry deformation of each segmented region. Meanwhile, we analyze the importance of these muscular areas, and a score level fusion strategy is exploited to optimize their weights by the genetic algorithm in the learning step. The support vector machine and the hidden Markov model are finally used to predict the expression label in 3D and 4D, respectively. The experiments are conducted on the BU-3DFE and BU-4DFE databases, and the results achieved clearly demonstrate the effectiveness of the proposed method.
Augmentation of BNP gene expression in atria by pressure overload in transgenic rats harbouring human renin and angiotensinogen genes (1999)

We studied the role of angiotensin II in pressure overload-induced B-type natriuretic peptide (BNP) gene expression by using a double transgenic rat (dTGR) model, in which transgenic rats for the human angiotensinogen and renin genes are crossed. Pressure overload produced by [Arg8]-vasopressin (AVP) infusion (i.v., 0.05 microg/kg/min for 2 h) in conscious, chronically instrumented rats, resulted in a significantly greater increase in BNP mRNA levels in the left atrium of the dTGR rats than in Sprague-Dawley (SD) control rats (3.6- vs 1.6-fold, p < 0.05), while in the left ventricle there was no significant difference between the strains. In dTGR rats, the early activation of the BNP gene expression was associated with a decrease in immunoreactive BNP levels in the atrium (27.5%, p < 0.05), but not in the ventricle. In SD rats, ir-BNP levels did not change significantly in either atria or ventricles in response to AVP infusion. These results show that the pressure overload-induced activation of BNP gene expression differs between atrial and ventricular myocytes in the dTGR model of experimental hypertension.
Results of in utero atrial septoplasty in fetuses with hypoplastic left heart syndrome (2008)

Technically successful atrial septal defect creation in fetuses with hypoplastic left heart syndrome and intact atrial septum results in atrial septal defects of varying size; defects of at least 3 mm in diameter appear to confer postnatal benefit.
AF-Biased CoFe Multilayer Films With FMR Frequency at 5 GHz and Beyond (2004)

Antiferromagnetic/ferromagnetic/antiferromagnetic (AF/F/AF) multilayers are investigated. The AF layer is 500-/spl Aring/-thick Ni/sub 50/Mn/sub 50/. Films are studied as a function of the thickness of the ferromagnetic layer (e/sub F/). Films with Ni/sub 80/Fe/sub 20/, Co/sub 50/Fe/sub 50/, and Co/sub 90/Fe/sub 10/ exhibit interfacial exchange coupling energy densities (J/sub ex/) of 0.22, 0.82, and 0.97 erg.cm/sup -2/ respectively. High J/sub ex/ ensures strong exchange coupling (H/sub ex/) and large uniaxial anisotropy fields (H/sub ua/) at 90/spl deg/ from the pinning direction. This promotes soft magnetism in CoFe films while they are not soft naturally. With e/sub F/=150 /spl Aring/,H/sub ua/ is of 570 and 755 Oe for Co/sub 50/Fe/sub 50/ and Co/sub 90/Fe/sub 10/, respectively. Thicknesses of /spl sim/0.3 /spl mu/m are also shown possible for practical use by means of multiple alternations (n /spl ges/ 20). Microwave characterizations confirm coherent reversible magnetization processes. Co/sub 50/Fe/sub 50/ multilayers are the best candidates with the highest /spl mu/' and the lowest /spl mu/'' at high frequency because an exceptional combination of a high 4/spl pi/M/sub s/ of 21 kG and a small damping factor of 0.015. ferromagnetic resonance frequencies of /spl sim/5 GHz are reported, and values of /spl sim/10 GHz are shown realistic, for the first time.