gebhart/patrepeval huggingface dataset:
Expression and Function of Ryanodine Receptors in Nonexcitable Cells (1996)

We have used reverse transcriptase-polymerase chain reaction to investigate the expression of ryanodine receptors in several excitable and nonexcitable cell types. Consistent with previous reports, we detected ryanodine receptor expression in brain, heart, and skeletal muscle. In addition, we detected ryanodine receptor expression in various other excitable cells including PC 12 and A7r5 cells. Several muscle cell lines (BC3H1, C2C12, L6, and Sol8) weakly expressed ryanodine receptor when undifferentiated but strongly expressed type 1 and type 3 ryanodine receptor isoforms when differentiated into a muscle phenotype. Only 2 (HeLa and LLC-PK1 cells) out of 11 nonexcitable cell types examined expressed ryanodine receptors. Expression of ryanodine receptors at the protein level in these cells was confirmed using [3H]ryanodine binding. We also investigated the function of ryanodine receptors in Ca2+ signaling in HeLa cells using single-cell Fura-2 imaging. Neither caffeine nor ryanodine caused a detectable elevation of cytoplasmic Ca2+ in single HeLa cells. However, ryanodine caused a significant decrease in the amplitude of Ca 2+ signals evoked by repetitive stimulation with ATP. These studies show that ryanodine receptors are expressed in some nonexcitable cell types and furthermore suggest that the ryanodine receptors may be involved in a subtle regulation of intracellular Ca2+ responses.
Interference in Visual Working Memory (2000)

This paper uses the theoretical distinction that has recently developed between the passive visual store and the active spatial rehearsal mechanism of the visuo-spatial component of working memory (VSSP). It examines the circumstances under which visual fields gain functional access to the passive visual store and seeks to cast light on the circumstances under which irrelevant visual fields interfere with concurrent visual processing. Experiment 1 contrasts a dynamic visual noise field with a static noise field and shows that the static field, in contrast to the dynamic noise field, causes no interference when presented concurrently with a visual task. Experiment 2 investigates the reason for this contrast and concludes that the static field is susceptible to decay and so fails to cause interference. Experiment 3 investigates further the circumstances under which dynamic visual noise causes interference and shows that manipulation of the number of changes within the noise field is also of importance in causing interference. The results allow further consideration of the characteristics of the VSSP.
Evaluation of Stainless Steel Nerve Caps for Palmar Digital Neurectomy (1982)

Stainless steel nerve caps for application to the proximal nerve stump during palmar digital neurectomy were examined. Six experimental horses and three clinical patients underwent palmar digital neurectomy using the capping technique. Good results were obtained on the experimental horses; however, when the caps were used on the clinical patients, lameness appeared in two of three horses after hard work. The caps were considered unacceptable for clinical use.
Sport players detection and tracking with a mixed network of planar and omnidirectional cameras (2009)

A generic approach is presented to detect and track people with a network of fixed and omnidirectional cameras given severely degraded foreground silhouettes. The problem is formulated as a sparsity constrained inverse problem. A dictionary made of atoms representing the silhouettes of a person at a given location is used within the problem formulation. A reweighted scheme is considered to better approximate the sparsity prior. Although the framework is generic to any scene, the focus of this paper is to evaluate the performance of the proposed approach on a basketball game. The main challenges come from the players' behavior, their similar appearance, and the mutual occlusions present in the views. In addition, the extracted foreground silhouettes are severely degraded due to the polished floor reflecting the players, and the strong shadow present in the scene. We present qualitative and quantitative results with the APIDIS dataset as part of the ICDSC sport challenge.
Time-temperature superposition and linear viscoelasticity of polybutadienes (1995)

International audience
Near-Infrared Luminescence of Supramolecular Species Consisting of Osmium(II)- and/or Ruthenium(II)-Polypyridine Components (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNear-Infrared Luminescence of Supramolecular Species Consisting of Osmium(II)- and/or Ruthenium(II)-Polypyridine ComponentsAlberto Juris, Vincenzo Balzani, Sebastiano Campagna, Gianfranco Denti, Scolastica Serroni, Gabriela Frei, and Hans U. GuedelCite this: Inorg. Chem. 1994, 33, 7, 1491–1496Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March 1994https://pubs.acs.org/doi/10.1021/ic00085a045https://doi.org/10.1021/ic00085a045research-articleACS PublicationsRequest reuse permissionsArticle Views258Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Dissecting kinetic pathways to formation of the fibrillar objects in molecular gels using synchrotron FT-IR (2015)

Infra-red synchrotron radiation is used to monitor the formation of fibrillar networks as sols of simple gelators become gels.
Early Ultrafast Ultrasound Imaging of Cerebral Perfusion correlates with Ischemic Stroke outcomes and responses to treatment in Mice (2020)

In the field of ischemic cerebral injury, precise characterization of neurovascular hemodynamic is required to select candidates for reperfusion treatments. It is thus admitted that advanced imaging-based approaches would be able to better diagnose and prognose those patients and would contribute to better clinical care. Current imaging modalities like MRI allow a precise diagnostic of cerebral injury but suffer from limited availability and transportability. The recently developed ultrafast ultrasound could be a powerful tool to perform emergency imaging and long term follow-up of cerebral perfusion, which could, in combination with MRI, improve imaging solutions for neuroradiologists. <b>Methods:</b> In this study, in a model of <i>in situ</i> thromboembolic stroke in mice, we compared a control group of non-treated mice (N=10) with a group receiving the gold standard pharmacological stroke therapy (N=9). We combined the established tool of magnetic resonance imaging (7T MRI) with two innovative ultrafast ultrasound methods, ultrafast Doppler and Ultrasound Localization Microscopy, to image the cerebral blood volumes at early and late times after stroke onset and compare with the formation of ischemic lesions<b>.</b><b>Results:</b> Our study shows that ultrafast ultrasound can be used through the mouse skull to monitor cerebral perfusion during ischemic stroke. In our data, the monitoring of the reperfusion following thrombolytic within the first 2 h post stroke onset matches ischemic lesions measured 24 h. Moreover, similar results can be made with Ultrasound Localization Microscopy which could make it applicable to human patients in the future. <b>Conclusion:</b> We thus provide the proof of concept that in a mouse model of thromboembolic stroke with an intact skull, early ultrafast ultrasound can be indicative of responses to treatment and cerebral tissue fates following stroke. It brings new tools to study ischemic stroke in preclinical models and is the first step prior translation to the clinical settings.
Predictive Clinicopathologic and Dynamic Contrast-Enhanced MRI Findings for Tumor Response to Neoadjuvant Chemotherapy in Triple-Negative Breast Cancer (2017)

Homogeneous enhancement of tumors on pre-NAC MRI and the presence of a concentric shrinkage pattern after NAC are associated with pCR in patients with TNBC. Residual tumor sizes on MRI and surgical specimens tended to show a stronger correlation in the pCR group or the concentric shrinkage group than in the non-pCR group or the dendritic shrinkage group.
Die pH‐Kontrolle in der mikrobiologischen Verfahrenstechnik (1964)

Abstract Es wurde eine sterilisierbare p H ‐Meßkette entwickelt, bei der Glas‐ und Bezugs‐elektrode kombiniert (Einstabmeßkette) in einer geschlossenen Fassung untergebracht sind und bei der sich der Überdruck einfach kompensieren läßt. Die schlagfesten Elektroden weisen günstige Eigenschaften hinsichtlich Hysterese‐Freiheit, Stabilität der Steilheit und der Nullpunktslage auch nach thermischer Belastung auf, so daß die Elektroden in dem Fermenter mitsterilisiert werden können. Die Sterilhaltung mikrobiologischer Züchtungssysteme ist damit gesichert. Der Aufbau eines Regelkreises zur automatischen p H ‐Kontrolle wird angegeben.
To Be Determined (2007)

Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text A. Scherer, "To Be Determined," in Photonic Metamaterials: From Random to Periodic, OSA Technical Digest (CD) (Optica Publishing Group, 2007), paper TuC2. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Determination of mitochondrial calcium content in hepatocytes by a rapid cellular-fractionation technique. α-adrenergic agonists do not mobilize mitochondrial Ca2+ (1984)

A rapid cellular fractionation technique [the preceding paper, Shears & Kirk (1984) Biochem. J., 219, 375-382] was employed to separate a mitochondria-rich fraction from hepatocytes within seconds. Mitochondrial Ca was estimated to be no more than 41% of total cell Ca. At least half of the mitochondrial Ca was present in an energy-dependent pool; 20% of total cell Ca was accessible to EGTA within 10s. The alpha-adrenergic agonist phenylephrine stimulated glycogen phosphorylase activity by 100% within 0.5 min and induced a loss of 20% of total cell Ca after 10 min from the EGTA-inaccessible pool. However, between 0.5 and 10 min after the addition of phenylephrine to hepatocytes there was no significant change in the Ca content of the mitochondria-rich fraction. Hepatocytes that were preloaded with Ca2+ during 90 min incubation at 0-4 degrees C expelled this cation during 20 min incubation at 37 degrees C. After this time, phenylephrine failed to alter the Ca content of a mitochondria-rich fraction. It is concluded that alpha-adrenergic agonists do not mobilize Ca2+ from hepatocyte mitochondria.
Generalised Pre-Coding Aided Spatial Modulation (2013)

A new Multiple Input Multiple Output (MIMO) transmission scheme termed as Generalised Pre-coding aided Spatial Modulation (GPSM) is proposed, where the key idea is that a particular subset of receive antennas is activated and the activation pattern itself conveys useful information. This is in contrast to the previously proposed Spatial Modulation (SM) schemes, which operated by activating a specific subset of transmit antennas. We provide both analytical and numerical results characterizing the GPSM scheme proposed for both conventional as well as for large-dimensional MIMO configurations subject to both realistic imperfect Channel State Information at the Transmitter (CSIT) and to the low-rank approximation invoked for large-dimensional MIMO configurations. We also design a so-called reinforcement matrix for attaining substantial performance improvements for our proposed GPSM scheme. Our investigations show that the GPSM scheme constitutes a flexible alternative to the state-of-the-art MIMO transmission schemes, especially because it is capable of achieving a high throughput. Moreover, the benefits of mapping information to the spatial domain rather than relying on conventional modulation has substantial benefits in the medium to high Signal to Noise Ratio (SNR) region. Quantitatively, GPSM is capable of supporting the same throughput as the conventional full-multiplexing gain based MIMO arrangements at an SNR gain of about 1dB at the same receiver complexity. Furthermore, the reinforcement matrix aided GPSM scheme attains a further 3-4dB performance improvement as compared to the conventional GPSM scheme.
Produkcja biokomponentów paliwowych drugiej i trzeciej generacji poprzez procesy termochemiczne – bioolej z mikroalg (2017)

Produkcja biokomponentw paliwowych drugiej i trzeciej generacji poprzez procesy termochemicznebioolej z
COMPLIFE 2007: 3rd International Symposium on Computational Life Science (2007)

The focus of the symposium is on computational methods for modeling, analysis, and exploration of all aspects of life sciences, ranging from effects on the molecular level to complex interactions networks. Of prime importance for this truly interdisciplinary event is the integration of the three disciplines: Computer Science, Biology, and Chemistry. These proceedings contains contributions regarding applications aspects as well as new theoretical developments.
The Importance of Cancer Cell Lines as in vitro Models in Cancer Methylome Analysis and Anticancer Drugs Testing (2013)

The Importance of Cancer Cell Lines as in vitro Models in Cancer Methylome Analysis and Anticancer Drugs Testing
Uncatalyzed, Regioselective Oxidation of Saturated Hydrocarbons in an Ambient Corona Discharge (2017)

Atmospheric pressure chemical ionization (APCI) in air or in nitrogen with just traces of oxygen is shown to yield regioselective oxidation, dehydrogenation, and fragmentation of alkanes. Ozone is produced from ambient oxygen in situ and is responsible for the observed ion chemistry, which includes partial oxidation to ketones and C-C cleavage to give aldehydes. The mechanism of oxidation is explored and relationships between ionic species produced from individual alkanes are established. Unusually, dehydrogenation occurs by water loss. Competitive incorporation into the hydrocarbon chain of nitrogen versus oxygen as a mode of ionization is also demonstrated.
Steroid hormone receptors in human squamous carcinoma cell lines (1984)

Specific estrogen, progesterone and androgen receptors were determined in 19 well-characterized squamous-cell carcinoma (SCC) cell lines. Ten of the lines were derived from patients with SCC of the larynx and nine were from patients with SCC originating in other areas of the head and neck. Estrogen receptors (ER) were found in seven of 10 cell lines derived from squamous cancers of the larynx (70%) but in only one of the nine SCC cell lines from sites other than larynx. Progesterone receptors (PGR) were more evenly distributed. Eight of the 10 laryngeal carcinoma cell lines (80%) and five of the nine non-laryngeal SCC lines (55%) had progesterone receptors. Only one cell line, UM-SCC-10B (derived from a recurrent carcinoma of the larynx) was found to express androgen receptors (AR). Expression of specific estrogen receptors was not dependent on the sex of the patients since lines from both males and females contained receptors. These results establish that squamous carcinoma cell lines may express specific steroid hormone receptors and that cell lines from cancers of the larynx (an organ known to be androgen-responsive) are more likely to express estrogen receptors than androgen receptors. From this initial survey it appears that there is a striking difference in estrogen and progesterone receptor content between SCC cell lines originating from larynx cancers and cell lines established from squamous carcinomas of other head and neck regions. The presence of estrogen receptors in a high proportion of laryngeal carcinoma cell lines suggests that hormonal therapy may be a useful adjunctive therapy in selected patients with cancer of the larynx.
Comedians in Cafes Getting Data (2020)

Social robots and autonomous social agents are becoming more ingrained in our everyday lives. Interactive agents from Siri to Anki's Cozmo robot include the ability to tell jokes to engage users. This ability will build in importance as in-home social agents take on more intimate roles, so it is important to gain a greater understanding of how robots can best use humor. Stand-up comedy provides a naturally-structured experimental context for initial studies of robot humor. In this preliminary work, we aimed to compare audience responses to a robotic stand-up comedian over multiple performances that varied robot timing and adaptivity. Our first study of 22 performances in the wild showed that a robot with good timing was significantly funnier. A second study of 10 performances found that an adaptive performance was not necessarily funnier, although adaptations almost always improved audience perception of individual jokes. The end result of this research provides key clues for how social robots can best engage people with humor.
Prediction of the Retention Behavior of lonizable Compounds in Reversed-Phase LC Using Factor-Analytical Modeling (1996)

Principal component factor analysis (PCFA) and target transformation factor analysis (TTFA) are used to examine the reversed-phase high-performance liquid chromatographic retention behavior of some neutral, basic, and acidic organic compounds in water-methanol-acetonitrile solvent systems at pH 7, 5, and 3. The ratios of the pairs of HA/A- (acid) or B/BH+ (base) are controlled by the buffered mobile phases, and this permits the factor analytical model to be extended to ionic compounds. Four factors are sufficient to reproduce the data within an error margin of 3%.
Expression of LINE-1 retroposons is essential for murine preimplantation development (2005)

In higher eukaryotes, reverse transcriptase (RT) activities are encoded by a variety of endogenous retroviruses and retrotransposable elements. We previously found that mouse preimplantation embryos are endowed with an endogenous RT activity. Inhibition of that activity by the non nucleosidic inhibitor nevirapine or by microinjection of anti-RT antibody caused early embryonic developmental arrest. Those experiments indicated that RT is required for early development, but did not identify the responsible coding elements. We now show that microinjection of morpholino-modified antisense oligonucleotides targeting the 5' end region of active LINE-1 retrotransposons in murine zygotes irreversibly arrests preimplantation development at the two- and four-cell stages; the overall level of functional RT is concomitantly downregulated in arrested embryos. Furthermore, we show that the induction of embryo developmental arrest is associated with a substantial reprogramming of gene expression. Together, these results support the conclusion that expression of LINE-1 retrotransposons is required for early embryo preimplantation development.
X-linked B lymphocyte defect in CBA/N mice. IV. Cellular and environmental influences on the thymus dependent IgG anti-sheep red blood cell response. (1979)

The primary thymus-dependent IgG anti-sheep red blood cell (SRBC) response of (CBA/N × DBA/2)F 1 male mice was 1 female mice. Adoptive transfers of activated T cells (ATC) and either bone marrow or anti-ϑ-treated spleen cells from F 1 mice into lethally irradiated recipients demonstrated that the functional helper-cell activity of ATC from immune-defective and normal F 1 mice was equivalent. By contrast, B lymphocytes of (CBA/N × DBA/2)F 1 male mice gave very poor or absent IgG anti-SRBC responses in this system, whereas the response of B cells from immunologically normal F 1 mice was excellent. The poor response of (CBA/N × DBA/2)F 1 male B cells was not improved by providing additional T cell help, suggesting that these cells were refractory to a large proportion of this help. Furthermore, increasing the number of donor B cells from immune-defective mice failed to influence materially the IgG anti-SRBC response. Although the response of (CBA/N × DBA/2)F 1 male primed B cells was considerably improved over that of unprimed cells, it remained less than the primary response of immunologically normal F 1 mice. The presence of a low but detectable SRBC response in (CBA/N × DBA/2)F 1 male mice suggests that the B cells of these mice, and by inference those of normal mice, can be triggered by antigen in the presence of ATC through a mechanism that is independent of Lyb 3 + , 5 + B cells. The large increase in the response of (CBA/N × DBA/2)F 1 male B cells to SRBC after priming suggests that Lyb 3 + , 5 + B cells are not necessary for the generation of memory B cells. An interesting difference in the environments of (CBA/N × DBA/2)F 1 vs (DBA/2 × CBA/N)F 1 male mice was demonstrated by reconstituting these mice with mixtures of ATC and B cells after lethal irradiation. In all cases, the IgG responses of the immune-defective F 1 mice were significantly less than those of the immunologically normal mice. Mechanisms that could explain these findings are discussed.
The D-Alanyl carrier protein in Lactobacillus casei: cloning, sequencing, and expression of dltC (1996)

The incorporation of D-alanine into membrane-associated D-alanyl-lipoteichoic acid in Lactobacillus casei requires the 56-kDa D-alanine-D-alanyl carrier protein ligase (Dcl) and the 8.9-kDa D-alanyl carrier protein (Dcp). To identify and isolate the gene encoding Dcp, we have cloned and sequenced a 4.3-kb chromosomal fragment that contains dcl (dltA). In addition to this gene, the fragment contains three other genes, dltB, d1tC, and a partial dltD gene. dltC (246 nucleotides) was subcloned from this region and expressed in Escherichia coli. The product was identified as apo-Dcp lacking the N-terminal methionine (8,787.9 Da). The in vitro conversion of the recombinant apo-Dcp to holo-Dcp by recombinant E. coli holo-ACP synthase provided Dcp which accepts activated D-alanine in the reaction catalyzed by Bcl. The recombinant D-alanyl-Dcp was functionally identical to native D-alanyl-Dcp in the incorporation of D-alanine into lipoteichoic acid. L. casei Dcp is 46% identical to the putative product of dltC in the Bacillus subtilis dlt operon (M. Perego, P. Glaser, A. Minutello, M. A. Strauch, K. Leopold, and W. Fischer, J. Biol. Chem. 270:15598-15606, 1995), and therefore, this gene also encodes Dcp. Comparisons of the primary sequences and predicted secondary structures of the L. casei and B. subtilis Dcps with that of the E. coli acyl carrier protein (ACP) were undertaken together with homology modeling to identify the functional determinants of the donor and acceptor specificities of Dcp. In the region of the phospho-pantetheine attachment site, significant similarity between Dcps and ACPs was observed. This similarity may account for the relaxed acceptor specificity of the Dcps and ACPs in the ligation Of D-alanine catalyzed by Dcl. In contrast, two Dcp consensus sequences, KXXVLDXLA and DXVKXNXD, share little identity with the rest of the ACP family and, thus, may determine the donor specificity of D-alanyl-Dcp in the D-alanylation of membrane-associated D-alanyl-lipoteichoic acid.