gebhart/patrepeval huggingface dataset:
From natural products to Alzheimer experimental therapeutics, eserine based drugs as symptomatic and disease altering agents (2010)

Summary Based on the hexahydropyrroloindole backbone of the natural product and alkaloid, (-)-physostigmine (eserine) – a short-acting non-selective reversible cholinesterase inhibitor, three series of compounds were designed and developed to overcome its pharmacokinetic and pharmacodynamic shortfalls to provide experimental therapeutics for Alzheimer’s disease (AD) and pharmacological tools to define brain function in health, aging and disease. Posiphen and analogues are amyloid-b precursor protein (APP) and amyloid-b peptide (Ab) lowering agents that are cholinergically inert but generate metabolites with anticholinesterase action. (-)-Bisnorcymserine and analogues are potent, centrally active, reversible, selective butyrylcholinesterase inhibitors, whereas (-)-phenserine and analogues are similar but selective acetylcholinesterase inhibitors, and each additionally lowers APP and Ab generation as a secondary action. Defining the clinical value of these agents in AD and related dementias is a key focus of current research. A partire da prodotti naturali per la terapia sperimentale nell’Alzheimer, farmaci a base di eserina come agenti in grado di alterare la malattia e la sintomatologia Riassunto Basandosi sullo scheletro esaidropirroloindolico del prodotto naturale e alcaloide (-)-fisostigmina (eserina), un inibitore reversibile non selettivo a breve durata d’azione della colinesterasi, sono state progettate e sviluppate tre serie di composti con duplice scopo: per superare le sue carenze farmacocinetiche e farmacodinamiche in modo da allestire terapie sperimentali per la malattia di Alzheimer (MA) e come strumenti farmacologici per definire le funzioni cerebrali in materia di salute, invecchiamento e patologia. Il Posiphen e analoghi, che promuovono l’abbassamento dei livelli della proteina precursore b-amiloide (APP) e del peptide b-amiloide (Ab), sono colinergicamente inerti ma generano metaboliti con azione anticolinesterasica. (-)-Bisnorcymserina e analoghi sono potenti, attivi a livello centrale, reversibili, inibitori selettivi della butirilcolinesterasi, mentre (-)-fenserina e analoghi sono simili ma inibitori selettivi dell’acetilcolinesterasi, e ciascuno abbassa ulteriormente la produzione di APP e di Ab come azione secondaria. Definire il valore clinico di questi agenti nella MA e nelle demenze correlate e un obiettivo chiave delle ricerche in corso.
On the anionic polymerization of dialkylaminoisoprenes, 4. Experimental observation of the unusual polymerization kinetics (1998)

The anionic polymerization of 5-(N,N-dialkylamino)isoprenes with sec-butyllithium was investigated in different solvents under various conditions. The homopolymerization can be described as a 2nd order reaction with respect to the monomer concentration, indicating that two monomer units are involved in the rate determining reaction. A mechanistic proposal to explain this unusual behaviour is based on the structure of amino-functional lithium organic compounds.
Improved crystal quality of non-polar a-plane GaN epi-layers directly grown on optimized hole-array patterned r-sapphire substrates (2019)

The characteristics of <italic>a</italic>-plane GaN films directly grown on silicon dioxide (SiO<sub>2</sub>) hole-array patterned <italic>r</italic>-sapphire substrates (HPSS) were investigated in this work.
In situ photoreactions of proteins in spectrometers leading to variations in signal intensities (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn situ photoreactions of proteins in spectrometers leading to variations in signal intensitiesD. Balasubramanian, P. Guptasarma, and Manni LuthraCite this: J. Am. Chem. Soc. 1992, 114, 5, 1877–1878Publication Date (Print):February 1, 1992Publication History Published online1 May 2002Published inissue 1 February 1992https://pubs.acs.org/doi/10.1021/ja00031a050https://doi.org/10.1021/ja00031a050research-articleACS PublicationsRequest reuse permissionsArticle Views65Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Quality control of measurements of pH, carbon dioxide tension, and total carbon dioxide in plasma. (1978)

We have measured total carbon dioxide in plasma with a new carbon dioxide analyzer, and compared the results with total carbon dioxide data derived from measurements of carbon dioxide tension and pH. The results agree sufficiently well to demonstrate that the new instrument provides a simple, efficient procedure for monitoring the precision and accuracy of pH, carbon dioxide tension, or total carbon dioxide in plasma.
Preclinical analysis of human mesenchymal stem cells: tumor tropism and therapeutic efficiency of local HSV-TK suicide gene therapy in glioblastoma (2019)

Glioblastoma are highly invasive and associated with limited therapeutic options and a grim prognosis. Using stem cells to extend current therapeutic strategies by targeted drug delivery to infiltrated tumors cells is highly attractive. This study analyzes the tumor homing and therapeutic abilities of clinical grade human mesenchymal stem cells (MSCs) in an orthotopic glioblastoma mouse model. Our time course analysis demonstrated that MSCs display a rapid targeted migration to intracerebral U87 glioma xenografts growing in the contralateral hemisphere within the first 48h hours after application as assessed by histology and 7T magnetic resonance imaging. MSCs accumulated predominantly peritumorally but also infiltrated the main tumor mass and targeted distant tumor satellites while no MSCs were found in other regions of the brain. Intratumoral application of MSCs expressing herpes simplex virus thymidine kinase followed by systemic prodrug application of ganciclovir led to a significant tumor growth inhibition of 86% versus the control groups (p<0.05), which translated in a significant prolonged survival time (p<0.05). This study demonstrates that human MSCs generated according to apceth's GMP process from healthy donors are able to target and provide a significant growth inhibition in a glioblastoma model supporting a potential clinical translation.
Mycobacterium avium Subspecies paratuberculosis Bacteremia in Type 1 Diabetes Mellitus: An Infectious Trigger? (2007)

Mycobacterium avium 
subspecies paratuberculosis (MAP) is the
established cause of paratuberculosis in
ruminants (i.e., Johne disease). The bacterium
is shed in the milk of infected cows
and survives pasteurization. Recently, an
association between MAP and Crohn disease
has been suggested, wherein MAP has
been found to persist in a cell wall–deficient
form, escaping clearance by the host
immune system.
ChemInform Abstract: SYNTH. VON 6H,11H‐PYRROLO(1,2‐B)BENZODIAZOCIN, EINEM NEUEN STICKSTOFFHALTIGEN HETEROCYCLUS (1972)

Abstract o‐Brommethyl‐benzonitril (I) reagiert mit Pyrrol‐kalium (II) zum N‐Alkylierungsprodukt (III), das mit Lithiumaluminiumhydrid zum primären Amin (IV) hydriert wird.
Genetic characterization and passage instability of a novel hybrid virulence plasmid in a ST23 hypervirulent Klebsiella pneumoniae (2022)

Hypervirulent variants of <i>Klebsiella pnuemoniae</i> (hvKP), which causes life-threatening infections, is a global priority pathogen and frequently harbours virulence plasmids. The virulence plasmids have emerged as the predominant vehicles carrying the major pathogenic determinants of hypermucoviscosity and hypervirulence phenotypes. In the present study, we characterized a novel virulence plasmid in AP8555, an ST23 hvKP strain, which induced a metastatic infection and fatal septic shock in a critically ill patient. The serum killing assay, the quantitative biofilm formation assay, the <i>G.mellonella</i> infection model, and the mouse lethality assay demonstrated that AP8555 was almost as virulent as the hvKP strain NUTH-K2044. The plasmid pAP855 could be conjugated to <i>Klebsiella quasipneumoniae</i> ATCC700603 and <i>E. coli</i> J53 at a frequency of 7.2× 10<sup>-5</sup> and 8.7× 10<sup>-7</sup>, respectively. Whole-genome sequencing and bioinformatics analysis confirmed that the plasmid was novel, clustered to the incompatibility type of IncHI1B/IncFIB/IncFII and presented high similarity to the pK2044 plasmid. In contrast, a 130-kb large-fragment insertion was observed on the plasmid, which introduced a genetic hybrid zone with multiple conjugation-related genes of type IV secretion systems (T4SS) and <i>CcdAB</i> toxin-antitoxin systems (TAS) to the plasmid. In the transconjugants, the presence of pAP855 had a negative impact on bacterial fitness, but enhancing the virulence-associated phenotypes. <i>In vitro</i> evolution experiments showed that pAP855 in the transconjugants could not be stably inherited after 10 days of passage. Our study not only reports a novel hybrid plasmid but also highlights the putative pathway of conjugative virulence plasmid formation and evolution by means of genetic rearrangement through sequence insertion. These findings indicate that structural versatility could contribute to the dissemination of cointegrate virulence plasmid, although the plasmid incurred a fitness cost. Therefore, continuous monitoring the acquisition of conjugative virulence plasmids may have critical value for plasmid research and increase awareness of hvKP.
The emerging role of MIR-146A in the control of hematopoiesis, immune function and cancer (2012)

MicroRNA (miRs) represent a class of small non-coding regulatory RNAs playing a major role in the control of gene expression by repressing protein synthesis at the post-transcriptional level. Studies carried out during the last years have shown that some miRNAs plays a key role in the control of normal and malignant hgematopoiesis. In this review we focus on recent progress in analyzing the functional role of miR-146a in the control of normal and malignant hematopoiesis. On the other hand, this miRNA has shown to impact in the control of innate immune responses. Finally, many recent studies indicate a deregulation of miR-146 in many solid tumors and gene knockout studies indicate a role for this miRNA as a tumor suppressor.
Polymeric Microgels as Potential Drug Delivery Vesicles (2010)

The temperature dependent volume phase change of cross-linked amphiphilic molecules (microgels) suggests their use as drug delivery vesicles. Drug particles aggregate in the slightly hydrophobic microgel interior. They are stored in equilibrium until the critical temperature (Tv) is reached where the volume phase change limits available space, thus expelling the drugs. This loading property of hydroxypropylcellulose (HPC) microgels was tested using amperometric analytical techniques. Small molecules inside microgels do not approach the electrode surface, which decreases current signal. A room temperature (Troom) flow amperometric measurement comparing microgel/paracetamol solution with control paracetamol samples yielded about 20 percent concentration reduction in the microgel sample. Results from the steady-state electrochemical experiment confirm the 20 percent concentration drop in the microgel sample compared to the control sample at Troom. Using the steady-state experiment with a cyclic temperature ramp from Troom to beyond Tv showed that the paracetamol concentration change between the temperature extremes was greater for the microgels than for the controls. An evolving aspect of the study is the characterization of microgel shrinkage from in situ, temperature controlled liquid AFM images as compared to previously completed DLS characterization of the same microgel sample.
Effect of Surface Treatment on the Mechanical Properties of Activated Carbon Fiber (1994)

Activated carbon fiber (ACF) is used as an adsorbent for water purification, and the application field and the amount will be expanded by improving the property. So, we tried to make the surface of ACF hydrophobic by heat-treating in hydrogen flow (H2) up to 1000°C and by treating with low temperature plasma of carbon tetra fluoride (CF4). And from the view point of keeping good handling quality of ACF, the change of its mechanical properties, due to surface treatment, such as tensile strength, was investigated in this paper.Followings are the results: (1) By heat treatment in H2 flow, weight of ACF decreased, while there were both cases where the diameter decreased and where the diameter did not change. It depended on the kind of ACF.(2) Tensile strength of heat-treated ACF became higher and had maximum point between 500 and 750°C of heat treatment temperature. The temperature for the peak point depended on the kind of original fibers.(3) By the heat treatment at 900°C in H2 flow, decrease in containing oxygen groups on the surface was confirmed from the ESCA results. It was also confirmed from the measurement of equilibrium adsorbed water that the surface of ACF was made hydrophobic.(4) By CF4 plasma treatment, the mechanical properties and the equilibrium adsorbed water of ACF were not changed substantially.
Complete Carbonylation offac-[Tc(H2O)3(CO)3]+ under CO Pressure in Aqueous Media: A Single Sample Story! (2000)

The rate constant of ligand exchange on the complex fac-[(99)Tc(H(2)O)(3)(CO)(3)](+) was determined by means of (13)C, (17)O, and (99)Tc NMR spectroscopy under pressurized conditions in aqueous media. After keeping the sample under CO pressure for an extended period, the formation of [(99)Tc(CO)(6)](+) could unambiguously be detected in the (13)C and (99)Tc NMR spectra.
Nuclear Magnetic Resonance (NMR) Imaging of Arnold–Chiari Type I Malformation with Hydromyelia (1983)

Saturation recovery nuclear magnetic resonance (NMR) images and metrizamide computed tomography (CT) scans were obtained in an adult patient with a clinical history suggestive of syringomyelia. Both NMR and CT studies showed low lying cerebellar tonsils. The CT study demonstrated central cavitation of the spinal cord from the midthoracic to midcervical levels but could not exclude an intramedullary soft tissue mass at the cervico-medullary junction. The NMR images in transverse, coronal, and sagittal planes demonstrated extension of an enlarged central spinal cord cerebrospinal fluid space to the cervico-medullary junction. This was felt to be strong evidence for exclusion of an intramedullary soft tissue mass and in favor of a diagnosis of Arnold-Chiari Type I malformation with hydromyelia. The noninvasive nature of spinal cord and cervico-medullary junction evaluation with NMR is emphasized.
Pseudomonas rhodesiae PF1: A New and Efficient Biocatalyst for Production of Isonovalal from α-pinene Oxide (2002)

AbstractA new bacterial strain, identified as Pseudomonas rhodesiae PF1 and deposited under the accession number CIP 107491, is presented. It is very active for the production of the acyclic compound Z-2-methyl-5-isopropyl-hexa-2,5-dien-1-al (isonovalal) from &#102 -pinene oxide. Enzyme synthesis is induced by culturing cells on &#102 -pinene; growing bacteria also have the ability to synthesize the epoxide derivative of &#102 -pinene. Isonovalal production was performed without aeration and with concentrated resting cells previously frozen at &#109 20°C, and subsequently thawed in a water-organic solvent, two-phase system. The organic layer was hexadecane, the volume ratio being 1:1. The best results achieved allowed recovery of c.a. 60 g/l organic solvent of isonovalal in 2.5 h operation, which is the most efficient process to date in the area of terpene biotransformations.KeywordsPseudomonas Rhodesiae Pf1 (CIP 107491)TerpenesBiotransformationα-pinene OxideIsonovalalBiphasic Medium
Tendon Transfer Fixation in the Foot and Ankle: A Biomechanical Study Evaluating Two Sizes of Pilot Holes for Bioabsorbable Screws (2003)

The purpose of this study was to compare the initial fixation strengths of bioabsorbable screws for tendon transfers in the foot and ankle when the pilot hole size varied. A 7 x 20 mm screw was used with 5.5 mm and 6.5 mm drill holes, and a 5 x 20 mm screw was used with 3.9 mm and 4.5 mm drill holes. Biomechanical testing was performed on each tendon transfer in cadaver specimens. A paired t-test showed no significant difference in pullout strength when pilot hole size varied between 79 to 93% of the screw size for the 7 mm screw and 78 to 90% of the screw size for the 5 mm screw. Previous studies have found a critical value of tendon tension equaling 50 N with passive dorsiflexion of the foot. With an average value of approximately 170 N, the 7 mm screw provided three times the requisite strength. The 5 mm screw provided 1.5 times the requisite strength, but the transfer was technically more difficult.
Development of Predictive Maintenance Interface Using Multiple Linear Regression (2018)

The common practice in most of the oil and gas organizations is to use periodic and preventive (time-based) maintenance techniques. These time-based maintenance techniques apply checking on equipment at regular intervals to avoid failures. However, they can lead to high maintenance cost with either over maintenance or unscheduled downtime. This issue can be encountered by using predictive maintenance which predicts future equipment failures ahead of time. This method performs maintenance based on actual operating condition of equipment which reduces maintenance cost and eliminates the need for periodic maintenance. The purpose of this work is to develop a user-friendly Graphical User Interface (GUI) application based predictive maintenance data analytic interface with the implementation of Multiple Linear Regression predictive maintenance technique. Multiple data sets of parameters of Booster Compressor (BC) are used on the proposed GUI to determine the accuracy of future prediction through implementation of Multiple Linear Regression technique.
USING TECHNOLOGY TO IMPROVE TRANSPORT SURVEY QUALITY. IN: TRANSPORT SURVEY QUALITY AND INNOVATION (2003)

This chapter reviews the four main topics that have been identified as either offering immense benefits or that will never replace traditional techniques such as in-person interview, (mail). The four topics are: the benefits: today and in the future; barriers to use; data issues; and utility.
A 100-km BOFDA Assisted by First-Order Bi-Directional Raman Amplification (2019)

We present, to our knowledge for the first time, a 100-km Brillouin Optical Frequency-Domain Analysis (BOFDA) employing a 200-km fiber loop. Compared to our previous publication, enhanced sensor length, sensor accuracy and spatial resolution are presented. The performance improvements are achieved by applying distributed Raman amplification (DRA) and a digital high-pass filter. We report on temperature measurements over sensing distances of 75 km and 100 km both with a 12.5-m spatial resolution. Temperature changes of 5 ° C have been measured along 75 km sensing fiber. A temperature change of 30 ° C has been detected at 99.5 km.
Outcomes of Atypical Spitz Tumors With Chromosomal Copy Number Aberrations and Conventional Melanomas in Children (2013)

Death due to melanoma in childhood (up to 20 y of age) is a rare event, with an average of 18 cases reported annually in the United States. In this study we evaluated 2 subgroups of high-risk melanocytic neoplasms in childhood, specifically atypical Spitz tumors (ASTs) with chromosomal copy number changes and conventional melanomas. We analyzed the clinical, histologic, and molecular features of all cases and performed the Fisher exact test, logistic regression, and multivariate analysis to evaluate features associated with aggressive clinical behavior in these cases. Among the ASTs, all of which had 1 or more chromosomal copy number aberrations, the presence of homozygous 9p21 deletions and a positive sentinel lymph node were each found to be correlated with tumor extension beyond the sentinel lymph node, with P-values of 0.046 and 0.01, respectively. Two patients with ASTs that had homozygous 9p21 deletions developed brain metastasis, one of whom died of disease. Among the 21 conventional melanomas, 3 patients developed distant metastasis and died of disease. Chromosomal copy number aberrations evaluated by fluorescence in situ hybridization were present in the majority of the cases (16/18). Among conventional melanomas, we did not identify any clinical, histologic, or molecular features associated with aggressive behavior. The presence of 8q24 gains was seen almost exclusively in 6 amelanotic small cell melanomas in children of whom 1 died of disease. Characteristic chromosomal copy number aberrations may occur in specific subtypes of melanocytic neoplasms in children and may help with the classification and prognostication of these rare tumors.
Enhanced Erythrocyte Apoptosis in Sickle Cell Anemia, Thalassemia and Glucose-6-Phosphate Dehydrogenase Deficiency (2002)

Erythrocyte diseases such as sickle cell anemia, thalassemia and glucose-6-phosphate dehydrogenase deficiency decrease the erythrocyte life span, an effect contributing to anemia. Most recently, erythro-cytes have been shown to undergo apoptosis upon increase of cytosolic Ca(2+) activity. The present study has been performed to explore whether sickle cell anemia, thalassemia and glucose-6-phosphate dehydrogenase deficiency enhance the sensitivity of erythrocytes to osmotic shock, oxidative stress or energy depletion, all maneuvers known to increase cytosolic Ca(2+) activity. To this end, annexin binding as an indicator of apoptosis has been determined by FACS analysis. Erythrocytes from healthy individuals, from patients with sickle cell anemia, thalassemia or glucose-6-phosphate dehydrogenase deficiency all responded to osmotic shock (up to 950 mOsm by addition of sucrose for 24 hours), to oxidative stress (up to 1.0 mM tetra-butyl-hydroxyperoxide tBOOH) and to energy depletion (up to 48 hours glucose deprivation) with enhanced annexin binding. However, the sensitivity of sickle cells and of glucose-6-phosphate dehydrogenase deficient cells to osmotic shock and of sickle cells, thalassemic cells and glucose-6-phosphate dehydrogenase deficient cells to oxidative stress and to glucose depletion was significantly higher than that of control cells. Annexin binding was further stimulated by Ca(2+) ionophore ionomycin with significantly higher sensitivity of sickle cells and glucose-6-phosphate dehydrogenase deficient cells as compared to intact cells. In conclusion, sickle cells, thalassemic cells and glucose-6-phosphate dehydrogenase deficient erythrocytes are more sensitive to osmotic shock, oxidative stress and/or energy depletion, thus leading to enhanced apoptosis of those cells. The accelerated apoptosis then contributes to the shortened life span of the defective erythrocytes.