gebhart/patrepeval huggingface dataset:
Modulators of endogenous cannabinoid system аs neuroprotectors (2010)

The neuroprotective action of endocannabinoid N-arachidonoyldopamine(AA-DA) in the model of apoptosis induced incultured rat cerebellar granule neurons by K+ deprivation wasinvestigated. The efficacy of simultaneous application of AA-DAwith fatty acid amide hydrolase inhibitor N-eicosapentaenoyldopamine(EPA-5HT) was also evaluated. It was shown thatAA-DA dose-dependently protects neurons, with maximaleffect of 65% survived cells observed at concentration 10 M.The use of submaximal concentration (5 M) of AA-DA incombination with EPA-5HT (10 M) led to significantenhancement of neuroprotection. The value of cell survival forthe combination exceeded such values for AA-DA alone in concentrationof 5 and 10 M, increasing the neuronal survival up to78%. It may be concluded that such combination allows toreduce efficient concentration of neuroprotector and to diminishits possible side effects. Thus, the simultaneous application ofendocannabinoid system modulators such as cannabinoidreceptor agonists and inhibitors of endocannabinoid hydrolysisappears to be effective approach for neural cells protection.
Towards real-time processors: Electro- and all-optical photonic neural networks (Conference Presentation) (2020)

While the data throughput of electronic processors is rather high (TFLOP range), the question arises whether optical neural networks (NN) enable a value proposition for zero-delay (real-time) processing? The answer might be ‘yes’, since once the network is trained, the time to perform an inference tasks is simply given by the time-of-flight of the photon through the processor’s NN. Here we discuss how the three functions of the perceptron (dot-product synaptic weighting, summation, and nonlinear thresholding) can be mapped onto a) optoelectronic [George et al. Opt.Exp. 2019], and b) all-optical hardware [Miscuglio et al. OMEx 2018]. The latter is realized via co-integration of phase-change-materials atop Silicon photonics. Once trained, the weights only require rare updating, thus saving power. Performance wise, such an integrated all-optical NN is capable of &lt; fJ/MAC using experimental demonstrated pump-probe [Waldecker et al, Nat. Mat. 2015] with a delay per perceptron being ~ps.
Protein O-Linked Mannose β-1,4-N-Acetylglucosaminyl-transferase 2 (POMGNT2) Is a Gatekeeper Enzyme for Functional Glycosylation of α-Dystroglycan (2016)

Disruption of the <i>O</i>-mannosylation pathway involved in functional glycosylation of α-dystroglycan gives rise to congenital muscular dystrophies. Protein <i>O</i>-linked mannose β-1,4-<i>N</i>-acetylglucosaminyltransferase 2 (POMGNT2) catalyzes the first step toward the functional matriglycan structure on α-dystroglycan that is responsible for binding extracellular matrix proteins and certain arenaviruses. Alternatively, protein <i>O</i>-linked mannose β-1,2-<i>N</i>-acetylglucosaminyltransferase 1 (POMGNT1) catalyzes the first step toward other various glycan structures present on α-dystroglycan of unknown function. Here, we demonstrate that POMGNT1 is promiscuous for <i>O</i>-mannosylated peptides, whereas POMGNT2 displays significant primary amino acid selectivity near the site of <i>O</i>-mannosylation. We define a POMGNT2 acceptor motif, conserved among 59 vertebrate species, in α-dystroglycan that when engineered into a POMGNT1-only site is sufficient to convert the <i>O</i>-mannosylated peptide to a substrate for POMGNT2. Additionally, an acceptor glycopeptide is a less efficient substrate for POMGNT2 when two of the conserved amino acids are replaced. These findings begin to define the selectivity of POMGNT2 and suggest that this enzyme functions as a gatekeeper enzyme to prevent the vast majority of <i>O</i>-mannosylated sites on proteins from becoming modified with glycan structures functional for binding laminin globular domain-containing proteins.
Impact de fortes concentrations en additif sur des séparations chirales en chromatographie en phase supercritique utilisant une phase stationnaire chirale tris (3,5-dimethylphenyl carbamate d’amylose. (2017)

National audience
Synthetic polyphosphate inhibits endogenous coagulation and platelet aggregation in vitro (2016)

Platelet-derived polyphosphate has previously been indicated to induce coagulation. However, industrially synthesized polyphosphate has been found to have different effects from those of the platelet-derived form. The present study investigated whether synthetic sodium polyphosphate inhibits coagulation using routine coagulation tests and thromboelastography. Synthetic polyphosphate was found to inhibit adenosine diphosphate-, epinephrine-, arachidonic acid-, ristocetin-, thrombin-, oxytocin- and pituitrin-induced platelet aggregation. The effects of synthetic polyphosphate in clotting inhibition were revealed by the analysis of clotting factor activity and platelet aggregation tests. Synthetic polyphosphate may inhibit platelet aggregation by reducing platelet calcium levels, as indicated by the results of flow cytometric analysis and high-throughput fluorescent screening. Furthermore, analysis of thromboxane (TX)B2 by ELISA indicated that synthetic polyphosphate reduces platelet aggregation by inhibiting the TXA2 signaling pathway. In conclusion, synthetic polyphosphate inhibits clotting factor activity and endogenous coagulation by reducing the levels of calcium ions and TXA2 to curb platelet aggregation.
Evidence for local feedback control of human milk secretion (1989)

Conference Article| February 01 1989 Evidence for local feedback control of human milk secretion ANN PRENTICE; ANN PRENTICE *M.R.C. Dunn Nutrition Unit, Cambridge, CB4 1XJ, U.K. Search for other works by this author on: This Site PubMed Google Scholar CAROLINE V. P. ADDEY; CAROLINE V. P. ADDEY †Hannah Research Institute, Ayr KA6 5HL, U.K. Search for other works by this author on: This Site PubMed Google Scholar COLIN J. WILDE COLIN J. WILDE †Hannah Research Institute, Ayr KA6 5HL, U.K. Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Received: June 20 1988 Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1989 Biochemical Society1989 Biochem Soc Trans (1989) 17 (1): 122. https://doi.org/10.1042/bst0170122 Article history Received: June 20 1988 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation ANN PRENTICE, CAROLINE V. P. ADDEY, COLIN J. WILDE; Evidence for local feedback control of human milk secretion. Biochem Soc Trans 1 February 1989; 17 (1): 122. doi: https://doi.org/10.1042/bst0170122 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1989 Biochemical Society1989 Article PDF first page preview Close Modal You do not currently have access to this content.
Dealing with the unexpected: consumer responses to direct-access BRCA mutation testing (2013)

Background. Inherited BRCA gene mutations convey a high risk for breast and ovarian cancer, but current guidelines limit BRCA mutation testing to women with early-onset cancer and relatives of mutation-positive cases. Benefits and risks of providing this information directly to consumers are unknown. Methods. To assess and quantify emotional and behavioral reactions of consumers to their 23andMe Personal Genome Service(®) report of three BRCA mutations that are common in Ashkenazi Jews, we invited all 136 BRCA1 and BRCA2 mutation-positive individuals in the 23andMe customer database who had chosen to view their BRCA reports to participate in this IRB-approved study. We also invited 160 mutation-negative customers who were matched for age, sex and ancestry. Semi-structured phone interviews were completed for 32 mutation carriers, 16 women and 16 men, and 31 non-carriers. Questions addressed personal and family history of cancer, decision and timing of viewing the BRCA report, recollection of the result, emotional responses, perception of personal cancer risk, information sharing, and actions taken or planned. Results. Eleven women and 14 men had received the unexpected result that they are carriers of a BRCA1 185delAG or 5382insC, or BRCA2 6174delT mutation. None of them reported extreme anxiety and four experienced moderate anxiety that was transitory. Remarkably, five women and six men described their response as neutral. Most carrier women sought medical advice and four underwent risk-reducing procedures after confirmatory mutation testing. Male carriers realized that their test results implied genetic risk for female relatives, and several of them felt considerably burdened by this fact. Sharing mutation information with family members led to screening of at least 30 relatives and identification of 13 additional carriers. Non-carriers did not report inappropriate actions, such as foregoing cancer screening. All but one of the 32 mutation-positive participants appreciated learning their BRCA mutation status. Conclusions. Direct access to BRCA mutation tests, considered a model for high-risk actionable genetic tests of proven clinical utility, provided clear benefits to participants. The unexpected information demonstrated a cascade effect as relatives of newly identified carriers also sought testing and more mutation carriers were identified. Given the absence of evidence for serious emotional distress or inappropriate actions in this subset of mutation-positive customers who agreed to be interviewed for this study, broader screening of Ashkenazi Jewish women for these three BRCA mutations should be considered.
Exposure to oxLDL impairs TGF-β activity in human tendon cells (2022)

Abstract Background: Hypercholesterolemia is a known risk factor for the development of acute or chronic tendon injuries, but the mechanisms which link cholesterol and tendon pathology are not completely known. Previous studies have shown that patients with hypercholesterolemia experience elevated levels of oxidized LDL (oxLDL), a molecule which triggers inflammation and collagenase activity. In this study we discovered a potential mechanism that may contribute to tendon damage by oxLDL. Methods: Isolated human tendon cells were incubated in the presence or absence of oxLDL. The influence of oxLDL on the expression level of key mRNA and proteins was examined using real time quantitative PCR, ELISA and Western blots. The activities of enzymes relevant to collagen synthesis and breakdown (lysyl oxidase and matrix metalloproteinases) were quantified using fluorometry. Finally, the isolated human tendon cells in a 3D construct were exposed to combinations of oxLDL and TGF-β to examine their interacting effects on collagen matrix remodeling. Results: The expression of key mRNAs including TGFB, COL1A1, DCN, and LOX were reduced in human tendon cells by oxLDL while MMP s were increased. The oxLDL reduced the activity of LOX, whereas conversely MMP activities were induced. COL1A1 synthesis and TGF-β secretion were also inhibited. Adding recombinant TGF-β reversed the effects of oxLDL on the expression of collagens and LOX . OxLDL also impaired collagen matrix remodeling, and adding TGF-β restored the native phenotype. Conclusions: Exposure oxLDL in patients with hypercholesterolemia may adversely affect the mechanical and structural properties of tendon tissue through a direct action of oxLDL on tendon cells, including impairment of TGF-β expression. This impairment leads to disturbed matrix remodeling and synthesis, thereby potentially leading to increased risk of acute or chronic tendon injury.
Scanning Tunneling Microscopy of Ordered Organic Monolayer Films on Si(001) (1998)

Scanning tunneling microscopy and optical spectroscopy techniques have been utilized to investigate the formation of ordered organic monolayer films on the (001) face of silicon. Cyclopentene and 1,5-cyclooctadiene both produce monolayer films that are ordered translationally and rotationally. The rotational orientations of the molecules arise from the directional interaction of the orbitals of the starting alkene with the orbital of the dimers comprising the reconstructed Si(001) surface, with the Si(001) surface acting as a template for determining the directionality of molecules in the subsequent organic film. Using single-domain Si(001) samples, it is shown that the molecular films also exhibit anisotropy in optical properties when measured on centimeter length scales.
Factors That Cause Trimethoprim Resistance in Streptococcus pyogenes (2014)

The use of trimethoprim in treatment of Streptococcus pyogenes infections has long been discouraged because it has been widely believed that this pathogen is resistant to this antibiotic. To gain more insight into the extent and molecular basis of trimethoprim resistance in S. pyogenes, we tested isolates from India and Germany and sought the factors that conferred the resistance. Resistant isolates were identified in tests for trimethoprim or trimethoprim-sulfamethoxazole (SXT) susceptibility. Resistant isolates were screened for the known horizontally transferable trimethoprim-insensitive dihydrofolate reductase (dfr) genes dfrG, dfrF, dfrA, dfrD, and dfrK. The nucleotide sequence of the intrinsic dfr gene was determined for resistant isolates lacking the horizontally transferable genes. Based on tentative criteria, 69 out of 268 isolates (25.7%) from India were resistant to trimethoprim. Occurring in 42 of the 69 resistant isolates (60.9%), dfrF appeared more frequently than dfrG (23 isolates; 33.3%) in India. The dfrF gene was also present in a collection of SXT-resistant isolates from Germany, in which it was the only detected trimethoprim resistance factor. The dfrF gene caused resistance in 4 out of 5 trimethoprim-resistant isolates from the German collection. An amino acid substitution in the intrinsic dihydrofolate reductase known from trimethoprim-resistant Streptococcus pneumoniae conferred resistance to S. pyogenes isolates of emm type 102.2, which lacked other aforementioned dfr genes. Trimethoprim may be more useful in treatment of S. pyogenes infections than previously thought. However, the factors described herein may lead to the rapid development and spread of resistance of S. pyogenes to this antibiotic agent.
Observations of quasars and cool stars. (1968)

IR observations of quasars and cool stars, discussing instrumentation using He cooled detectors
Biological removal of pyritic sulfur from coal by the thermophilic organism Sulfolobus acidocaldarius (1985)

More than 90% of initial pyritic sulfur was removed from bituminous coal samples (containing 2.1% pyritic sulfur) using the thermophilic organism Sulfolobus acidocaldarius. Microbial desulfurization rate was improved nearly ten fold by adjusting the N/P and N/Mg ratios in the nutrient medium. Environmental conditions were optimized. The optimal values of temperature and pH were 70 degrees C and 1.5, respectively. The influence of certain process variables (such as coal pulp density, particle size, and initial cell number density) on the rate of pyritic sulfur removal were determined. A pulp density of 20%, particle size of D (p) < 48 microm, and an initial cell number density of 10(12) cells/g pyrite in coal were found to be optimal. The carbon dioxide enriched air did not improve the rate of pyritic sulfur removal compared to pure air at 10% pulp density of coal samples containing 2.1% pyritic sulfur. The kinetics of microbial leaching of pyritic sulfur from coal was investigated. The rate of leaching was found to be first order with respect to pyritic sulfur concentration in the reaction medium.
Hexagonal Boron Nitride UDMA Resin Composites (2018)

Objectives: To evaluate the potential of Hexagonal Boron Nitride as a reinforcing filler for light curable UDMA resin composites. Methods: Experimental resin composite formulated by incorporating 40 wt% hydroxyapatite into a UDMA monomer matrix with a photo-initiator system of CQ and DMAEM was used as control for the present study. Four hexagonal Boron Nitride (hBN) resin composites were fabricated by introducing 0.25 and 0.5 wt% of two hBN with difference morphology into the control. Cylindrical specimens (5 mm x 1/2 mm) were light cured and the real time transmitted irradiance at the bottom specimen surface was recorded using a calibrated spectrometer (MARC Resin Calibrator). The degree of conversion immediate and 7 d after cured (DC0 and DC7d), post-irradiance hardness (VHN0 and VHN 7d) and 7 d post-cured flexural strength were measured by a FTIR spectroscopy, a Vickers indenter and a universal testing machine respectively (n=5). Difference between the results was analysed using one way ANOVA (p&lt;0.05) followed by Tukey`s post hoc test. Results: The presence of hBN did not change the overall shape of the irradiation curve, but resulted in a reduction in the maximum transmitted irradiance (Imax) at the bottom sample surface compared with control. Sample thickness in contract had a dramatic influence on Imax. The addition of hBN did not significantly change DC for the 1 mm specimens; however, 0.5 wt% h-BN has a detrimental influence on DC for the 2 mm specimens. Post-irradiance hardness increased after 7 d, but no significant difference was noticed between groups. The addition of hBN led to a significant increase in flexural strength compared with the control group. Conclusions: Small amount of hBN did not change the curability of experimental UDMA resin composite but led to increased flexural strengths indicating that hBN has the potentials as a reinforcing filler for dental resin composites.
An MMIC wide-band doubly balanced resistive mixer with an active IF balun (2010)

In this paper, we present a wideband doubly-balanced resistive mixer fabricated using a 0.5 µm GaAs p-HEMT process. Three baluns are employed in the mixer. Local oscillator (LO) and radio frequency (RF) baluns operating over an 8 to 20 GHz range were implemented with Marchand baluns. In order to reduce the chip size, the Marchand baluns were realized using a meandering multi-coupled line, and inductor lines were inserted to compensate for the meandering effect. Over a frequency range of 8 to 20 GHz, the amplitude and phase unbalances of the LO and RF baluns were less than 1 dB and 6°, respectively. A 0.3 × 0.5 mm2 IF balun was implemented through a DC-coupled differential amplifier. The measured amplitude and phase unbalances were less than 1 dB and 5°, respectively, from DC to 7 GHz. And the output third-order intercept (OIP3) and PldB, at a frequency of 1 GHz, were 18 dBm and 6 dBm, respectively. The mixer is 1.7 × 1.8 mm2 in size, has a conversion loss of 5 to 11 dB, and an OIP3 of +10 to +15 dBm at 16 dBm LO power for the operating bandwidth.
The Suitability of Bovine‐Lens Leucine Aminopeptidase for Sequence Analysis and Limited Hydrolysis of Polypeptides (1973)

In the present paper we report findings showing the suitability of recrystallized bovine‐lens leucine aminopeptidase as a tool for peptide chemistry. The enzyme is homogeneous with respect to hydrodynamic, chromatographic and electrophoretic separation criteria. It is not contaminated by endopeptidases. The insulin B‐chain ( S ‐sulfonate) served as model substrate for sequence studies. The initial progress curves of the liberation of the first six amino acids resulted in a sequence expected from the known primary structure. The complete resistance of imido bonds towards the action of leucine aminopeptidase has been proved. Many commercially available protein preparations are found to be contaminated by endopeptidases. We developed an endopeptidase‐detection method in which the leucine aminopeptidase serves as a sensitivity amplifier.
Predicting Southern Pine Beetle Infestation Risk from Neighborhood History and Environmental Conditions (2008)

Predicting Southern Pine Beetle Infestation Risk from Neighborhood History and Environmental Conditions.(Under the direction of Fred Paul Hain
Analytical design of 160 Gbits/s densely dispersion-managed optical fiber transmission systems using Gaussian and raised cosine RZ ansätze (2004)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text A. Labruyère, P. T. Dinda, A. B. Moubissi, K. Nakkeeran, Y. H. C. Kwan, and P. K. A. Wai, "Analytical design of 160 Gbits/s densely dispersion-managed optical fiber transmission systems using Gaussian and raised cosine RZ ansätze," in Nonlinear Guided Waves and Their Applications, Technical Digest on CD (Optica Publishing Group, 2004), paper MC45. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Minimum 10-Year Follow-up of Patients After an Acute, Isolated Posterior Cruciate Ligament Injury Treated Nonoperatively (2013)

Long-term results after an isolated PCL injury show that patients remain active, have good strength and full knee range of motion, and report good subjective scores. The prevalence of moderate to severe osteoarthritis was 11%. Results were not different based on PCL laxity grade. This natural history study of nonoperatively treated PCL injuries can serve as a baseline for comparison with patients treated with PCL reconstruction.
INVOLVEMENT OF HYPOGASTRIC AND PELVIC NERVES FOR CONVEYING CYSTITIS INDUCED NOCICEPTION IN CONSCIOUS RATS (2001)

These results confirm the previously reported antinociceptive effect of systemic morphine in this model of cyclophosphamide cystitis, suggest that this antinociceptive action is completely located at the spinal site and most importantly demonstrate by the pharmacological approach and behavioral analysis that nociceptive sensations originating from the bladder are conveyed by hypogastric and pelvic nerves in this cyclophosphamide cystitis model in the conscious rat.
Convex Hull Aided Registration Method (CHARM) (2016)

Non-rigid registration finds many applications such as photogrammetry, motion tracking, model retrieval, and object recognition. In this paper we propose a novel convex hull aided registration method (CHARM) to match two point sets subject to a non-rigid transformation. First, two convex hulls are extracted from the source and target respectively. Then, all points of the point sets are projected onto the reference plane through each triangular facet of the hulls. From these projections, invariant features are extracted and matched optimally. The matched feature point pairs are mapped back onto the triangular facets of the convex hulls to remove outliers that are outside any relevant triangular facet. The rigid transformation from the source to the target is robustly estimated by the random sample consensus (RANSAC) scheme through minimizing the distance between the matched feature point pairs. Finally, these feature points are utilized as the control points to achieve non-rigid deformation in the form of thin-plate spline of the entire source point set towards the target one. The experimental results based on both synthetic and real data show that the proposed algorithm outperforms several state-of-the-art ones with respect to sampling, rotational angle, and data noise. In addition, the proposed CHARM algorithm also shows higher computational efficiency compared to these methods.