gebhart/patrepeval huggingface dataset:
GLS hyperactivity causes glutamate excess, infantile cataract and profound developmental delay (2018)

Loss-of-function mutations in glutaminase (GLS), the enzyme converting glutamine into glutamate, and the counteracting enzyme glutamine synthetase (GS) cause disturbed glutamate homeostasis and severe neonatal encephalopathy. We report a de novo Ser482Cys gain-of-function variant in GLS encoding GLS associated with profound developmental delay and infantile cataract. Functional analysis demonstrated that this variant causes hyperactivity and compensatory downregulation of GLS expression combined with upregulation of the counteracting enzyme GS, supporting pathogenicity. Ser482Cys-GLS likely improves the electrostatic environment of the GLS catalytic site, thereby intrinsically inducing hyperactivity. Alignment of +/-12.000 GLS protein sequences from >1000 genera revealed extreme conservation of Ser482 to the same degree as catalytic residues. Together with the hyperactivity, this indicates that Ser482 is evolutionarily preserved to achieve optimal-but submaximal-GLS activity. In line with GLS hyperactivity, increased glutamate and decreased glutamine concentrations were measured in urine and fibroblasts. In the brain (both grey and white matter), glutamate was also extremely high and glutamine was almost undetectable, demonstrated with magnetic resonance spectroscopic imaging at clinical field strength and subsequently supported at ultra-high field strength. Considering the neurotoxicity of glutamate when present in excess, the strikingly high glutamate concentrations measured in the brain provide an explanation for the developmental delay. Cataract, a known consequence of oxidative stress, was evoked in zebrafish expressing the hypermorphic Ser482Cys-GLS and could be alleviated by inhibition of GLS. The capacity to detoxify reactive oxygen species was reduced upon Ser482Cys-GLS expression, providing an explanation for cataract formation. In conclusion, we describe an inborn error of glutamate metabolism caused by a GLS hyperactivity variant, illustrating the importance of balanced GLS activity.
Genetic and phenotypic parameters for carcass and meat quality traits in commercial crossbred pigs1 (2014)

Pork quality and carcass characteristics are now being integrated into swine breeding objectives because of their economic value. Understanding the genetic basis for these traits is necessary for this to be accomplished. The objective of this study was to estimate phenotypic and genetic parameters for carcass and meat quality traits in 2 Canadian swine populations. Data from a genomic selection study aimed at improving meat quality with a mating system involving hybrid Landrace × Large White and Duroc pigs were used to estimate heritabilities and phenotypic and genetic correlations among them. Data on 2,100 commercial crossbred pigs for meat quality and carcass traits were recorded with pedigrees compromising 9,439 animals over 15 generations. Significant fixed effects (company, sex, and slaughter batch), covariates (cold carcass weight and slaughter age), and random additive and common litter effects were fitted in the models. A series of pairwise bivariate analyses were implemented in ASReml to estimate phenotypic and genetic parameters. Heritability estimates (±SE) for carcass traits were moderate to high and ranged from 0.22 ± 0.08 for longissimus dorsi muscle area to 0.63 ± 0.04 for trimmed ham weight, except for firmness, which was low. Heritability estimates (±SE) for meat quality traits varied from 0.10 ± 0.04 to 0.39 ± 0.06 for the Minolta b* of ham quadriceps femoris muscle and shear force, respectively. Generally, most of the genetic correlations were significant (P < 0.05) and ranged from low (0.18 ± 0.07) to high (-0.97 ± 0.35). There were high negative genetic correlations between drip loss with pH and shear force and a positive correlation with cooking loss. Genetic correlations between carcass weight (both hot and cold) with carcass marbling were highly positive. It was concluded that selection for increasing primal and subprimal cut weights with better pork quality may be possible. Furthermore, the use of pH is confirmed as an indicator for pork water-holding capacity and cooking loss. The heritabilities of carcass and pork quality traits indicated that they can be improved using traditional breeding methods and genomic selection, respectively. The estimated genetic parameters for carcass and meat quality traits can be incorporated into the breeding programs that emphasize product quality in these Canadian swine populations.
Paper based microfluidic aptasensor for food safety (2017)

ABSTRACT Food analysis is requiring rapid, accurate, sensitive and cost-effective methods to monitor and guarantee the safety and quality to fulfill the strict food legislation and consumer demands. In our study, a nano-materials enhanced multipurpose paper based microfluidic aptasensor was demonstrated as a sensing tool for accurate detection of food allergens and food toxins. Graphene oxide (GO) and specific aptamer - functionalized quantum dots (QDs) were employed as probes, the fluorescence quenching and recovering of the QDs caused by the interaction among GO, aptamer-functionalized QDs and the target protein were investigated to quantitatively analyze the target concentration. The homogenous assay was performed on the paper based microfluidic chip, which significantly decreased the sample and reagent consumptions and reduced the assay time. Egg white lysozyme, ß-conglutin lupine and food toxins, okadaic acid and brevetoxin standard solutions and spiked food samples were successfully assayed by the presented aptasensor. Dual-target assay was completed within 5 min, and superior sensitivities were achieved when testing the samples with commercial ELISA kits side by side.
Informational Efficiency with Trading Constraints: A Characterization (2020)

Related DatabasesWeb of Science You must be logged in with an active subscription to view this.Article DataHistorySubmitted: 14 February 2020Accepted: 18 August 2020Published online: 13 October 2020Keywordsinformational efficiency, trading constraints, no arbitrage, no dominance, equilibrium, representative agents, martingales measures, local martingalesAMS Subject HeadingsG11, G12, G14, D52, D53Publication DataISSN (online): 1945-497XPublisher: Society for Industrial and Applied MathematicsCODEN: sjfmbj
Supplementary Table 1 from Purity Independent Subtyping of Tumors (PurIST), A Clinically Robust, Single-sample Classifier for Tumor Subtyping in Pancreatic Cancer (2023)

&lt;p&gt;Table S1&lt;/p&gt;
Anticipated Alternative Policy Rate Paths in Policy Simulations (2011)

This paper specifies a new convenient algorithm to construct policy projections conditional on alternative anticipated policy-rate paths in linearized dynamic stochastic general equilibrium (DSGE) models, such as Ramses, the Riksbank’s main DSGE model. Such projections with anticipated policy-rate paths correspond to situations where the central bank transparently announces that it, conditional on current information, plans to implement a particular policyrate path and where this announced plan for the policy rate is believed and then anticipated by the private sector. The main idea of the algorithm is to include among the predetermined variables (the “state”of the economy) the vector of nonzero means of future shocks to a given policy rule that is required to satisfy the given anticipated policy-rate path.
An all-digital real-time optical coherence tomographic system (2004)

We developed an all-digital OCT system capable of providing real-time functional and structural information. Two different digital approaches are investigated and compared using simulated and phantom data.
Real-time application of IMC-PID-FO multi-loop controllers on the coupled tanks process (2021)

The coupled tanks process is a two input-two output system. It presents a nonlinear behavior and interactions characteristic. After the nonlinear model is obtained, it is linearized around an operating point. A fractional-order proportional–integral–derivative based on the internal model control paradigm (1DOF-IMC-PID-FO) multi-loop controller is determined without considering the interactions, and a fractional-order proportional–integral–derivative based on the 2-degree-of-freedom internal model control structure (2DOF-IMC-PID-FO) multi-loop controller is determined by considering the interactions. Thus, an interactions reduction effect controller is calculated. Both controllers are implemented on a real-time process using the Real Time Windows Target of MATLAB. The objective of the control is to maintain the water level in the lower tanks at desired values. In the experiment, setpoint tracking and disturbance rejection tests are carried out to assess the performance of both 1DOF and 2DOF-IMC-PID-FO multi-loop controllers.
Equivalence in knowledge representation: automata, recurrent neural networks, and dynamical fuzzy systems (1999)

Neurofuzzy systems-the combination of artificial neural networks with fuzzy logic-have become useful in many application domains. However, conventional neurofuzzy models usually need enhanced representation power for applications that require context and state (e.g., speech, time series prediction, control). Some of these applications can be readily modeled as finite state automata. Previously, it was proved that deterministic finite state automata (DFA) can be synthesized by or mapped into recurrent neural networks by directly programming the DFA structure into the weights of the neural network. Based on those results, a synthesis method is proposed for mapping fuzzy finite state automata (FFA) into recurrent neural networks. Furthermore, this mapping is suitable for direct implementation in very large scale integration (VLSI), i.e., the encoding of FFA as a generalization of the encoding of DFA in VLSI systems. The synthesis method requires FFA to undergo a transformation prior to being mapped into recurrent networks. The neurons are provided with an enriched functionality in order to accommodate a fuzzy representation of FFA states. This enriched neuron functionality also permits fuzzy parameters of FFA to be directly represented as parameters of the neural network. We also prove the stability of fuzzy finite state dynamics of the constructed neural networks for finite values of network weight and, through simulations, give empirical validation of the proofs. Hence, we prove various knowledge equivalence representations between neural and fuzzy systems and models of automata.
Experimental and Computational Study of Area and Perimeter Contributions to Radiometer Forces (2008)

The relative contribution to the radiometric force of the area and perimeter of the vane is studied experimentally and numerically. Experimentally, a circular vane, a low‐aspect rectangular vane, and a high‐aspect rectangular vane were all tested on a force balance, with nano‐Newton resolution, placed in a stagnant gas. The computational results were obtained through 2‐D simulations using the direct simulation Monte Carlo method, as well as a discrete ordinate solution of the ES model kinetic equation. Gas pressure was varied from 0.006 to 6 Pa, which was a broad enough range to observe the characteristic peak force production of a radiometer in the transition regime, where the peak occurs at Kn ∼0.1. It was found that the area of a radiometer vane is responsible for a significant amount of the total force production through a wide range of operating pressures. It is only at the highest background pressures, well after force production has peaked, that the vane perimeter appears to dominate the operation of the radiometer.
Randomized Speedup of the Bellman–Ford Algorithm (2012)

We describe a variant of the Bellman–Ford algorithm for single-source shortest paths in graphs with negative edges but no negative cycles that randomly permutes the vertices and uses this randomized order to process the vertices within each pass of the algorithm. The modification reduces the worst-case expected number of relaxation steps of the algorithm, compared to the previously-best variant by Yen (1970), by a factor of 2/3 with high probability. We also use our high probability bound to add negative cycle detection to the randomized algorithm.
Iodine Poisoning Counteracted by Thiosulphate. (1928)

2 p. Reprinted from: Proceedings of the Society for Experimental Biology and Medicine, 1928, Vol. 25, pp. 784-785
Failure of Isradipine to Reduce Infarct Size in Mouse, Gerbil, and Rat Models of Cerebral Ischemia (1995)

The L-type calcium channel blocker isradipine was devoid of neuroprotective activity in focal and global models of cerebral ischemia in three species of normotensive animals. These results were compared with data for the novel calcium channel blocker SB201823A, which exhibited a significant effect after pre- and postocclusion administration in the mouse model of permanent focal ischemia.
Sustainable One-Step Conversion of Soluble NaVO3 into CaV2O4 through Molten Salt Electrolysis (2019)

A simple and green route for the preparation of CaV2O4 through one-step incomplete electrochemical reduction of soluble NaVO3 in CaCl2 molten salt is proposed. NaVO3 was found to exist in the form of Ca2VO4Cl in the molten salt with VO43− as the reactive ion. CaV2O4 possessing a surface carbon layer and rod structure was obtained by adding CaCO3 during electrolysis. The electrochemical reduction mechanism was also analyzed, and the results indicated that VO43− was incompletely reduced to VO2− at about −1.23 V (vs. Ag/AgCl) by a two-electron transfer process. VO2− ions then combined with Ca2+ to form CaV2O4. Meanwhile, CO32− was reduced to carbon in a two-step process.
Development of Changeable Young's Modulus with Good Mechanical Properties in β-Type Ti-Cr-O Alloys (2013)

A novel β-type titanium alloy with a changeable Youngs modulus, that is, with a low Young's modulus to prevent the stress-shielding effect for patients and a high Young's modulus to suppress springback for surgeons, should be developed in order to satisfy the conflicting requirements of both the patients and surgeons in spinal fixation operations. In this study, the oxygen content in ternary Ti-11Cr-O alloys was optimized in order to achieve a large changeable Young's modulus with good mechanical properties for spinal fixation applications. The increase in Youngs moduli of all the examined alloys by cold rolling is attributed to the deformation-induced ω-phase transformation which is suppressed by oxygen. Among the examined alloys, the Ti-11Cr-0.2O alloy exhibits the largest changeable Youngs modulus and a high tensile strength with an acceptable plasticity under both solution-treated (ST) and cold-rolled (CR) conditions. Therefore, the Ti-11Cr-0.2O alloy, which shows a good balance among a changeable Youngs modulus, high tensile strength and good plasticity, is considered a potential candidate for spinal fixation applications.
Structure selection algorithm for less musical-noise generation in integration systems of beamforming and spectral subtraction (2009)

In this paper, we propose an appropriate structure selection algorithm for less musical-noise generation in integration methods of microphone array and spectral subtraction. In our previous work, we have analyzed musical-noise reduction structure in integration methods of microphone array and spectral subtraction based on higher order statistics. However, that analysis can be applied to only Gaussian and super-Gaussian noises. In this paper, we extend the analysis into sub-Gaussian noise case. As a result of the extended analysis, we find the fact that an appropriate structure is depending on the type of input noise. Based on this fact, we propose an appropriate structure selection algorithm in integration methods of microphone array and spectral subtraction for less musical-noise generation. The effectiveness of the proposed algorithm are shown via a computer simulation.
PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS (1994)

Four different methods of DNA extraction from formalin-fixed, paraffin-embedded tissues were compared for their ability to produce DNA suitable as a template for polymerase chain reaction (PCR). Seven paraffin-embedded rhabdomyosarcoma tissue blocks from the 1950s and one from 1960 were treated with the following extraction methods: 1. Proteinase K digestion and phenol/chloroform extraction; 2. Proteinase K digestion followed by boiling to inactivate the enzyme; 3. Proteinase K digestion, addition of Chelex-100, followed by boiling; and 4. Proteinase K digestion and Prep-A-Gene purification. DNA extracted by methods 1 and 2 was degraded, but DNA of high molecular weight was recovered in every sample extracted by methods 3 and 4 - even though some degradation was observed. Extracted DNA was used as a template for PCR amplification of exon 4 of the PAX-3 gene. PCR was successful in 7 out of 8 samples prepared using methods 3 and 4, producing levels of amplified product equivalent to those obtained with control DNA obtained from fresh lymphocytes. Only very weak products were found in samples prepared by methods 1 (2 out of 8) and 2 (4 out of 8). These results indicate that chelation of polyvalent ions (Chelex-100 method) or obviating the need for boiling (Prep-A-Gene) may protect DNA during extraction.
Optimization of Ecosystem Services of Shanghai Urban–Suburban Street Trees Based on Low-Carbon Targets (2021)

Road traffic carbon emissions are an important cause of global warming, and street trees play an important role in regulating road carbon emissions. During urbanization, major differences in the planting management modes and growth status of the street trees in urban–suburban gradient may exist, leading to significant differences in the low-carbon values of the street trees in urban–suburban gradient. Based on this, this study took two typical urban–suburban gradient zones in Shanghai as an example to analyze the changes in the characteristics of street tree species, planting density, tree sizes, and low-carbon contribution with urban and rural changes, and proposed strategies for optimizing the low-carbon contribution of urban street trees. The results showed that, from the inner ring to the outer ring and the suburban ring, the proportion of London plane tree gradually changed from 82% to 11%, and the proportion of the camphor tree gradually changed from 9% to 70%; the average DBH of the trees gradually decreased from 28.81 to 23.74 cm. The number of plantings per unit road length gradually increased, and the number of plantings per unit area gradually decreased; therefore, the average low-carbon contribution of urban–suburban street trees is not significant, but the low-carbon contribution of upper street trees per unit area is higher, and suburban unit street trees have a higher low-carbon contribution. Finally, this article proposes different optimization strategies for future urban micro-renewal and suburban new-city construction.
Production of T-helper cell subsets and cytokines by lymphocytes from patients with chronic mucocutaneous candidiasis (1996)

Chronic mucocutaneous candidiasis (CMC) is a heterogeneous group of disorders characterized by recurrent and persistent superficial candidal infections. Cytokine-induced dysregulation of T-helper cell function has been described in other immune-deficient states but has not been studied in CMC patients. We studied T-helper cell subsets by flow cytometry and cytokine production by stimulated lymphocytes in six CMC patients, two healthy pediatric controls, and five healthy adult controls. Peripheral blood lymphocytes were stimulated in vitro with phytohemagglutinin or Candida albicans extract, and the production of interleukin-2R (IL-2R), IL-4, IL-10, and gamma interferon in the supernatants was measured by enzyme-linked immunosorbent assay. CMC patients had a decrease in the CD29+/CD29+ cell population compared with the numbers in controls (P &lt; 0.02). The percentage of CD4+/CD45RA+ cells was greater in patients than in controls, but the difference was not significant. There was no difference in the production of IL-10 or gamma interferon by the patient lymphocytes. CMC patients produced more IL-4 than the controls (P &lt; 0.001), whereas the controls tended to produce more IL-2R than the patients (P = 0.19). These findings support the concept that a decrease in CD4+/CD29+ T-helper inducer cells along with T-helper cell dysregulation may lead to defective memory responses to antigens in CMC patients and a decrease in cell-mediated immunity due to inhibition of TH1 cells by increased levels of IL-4.
Oral Communication Capabilities of Purchasing Managers: Measurement and Typology (2006)

SUMMARY As supply chain management (SCM) becomes increasingly important, the sharing of information and oral communication has also increased in significance. This has resulted in the need for an instrument to measure managers' oral communication capabilities. The main objectives of this paper are to identify the dimensions of the oral communication capability and to develop an instrument to measure this capability. To achieve these goals, a survey was conducted among German and Spanish buyers and supply managers. The results indicate that the oral communication capability construct has a second‐order structure with three dimensions: the ability to pass on information, the ability to persuade and the ability to listen and understand. Based on these results, the Oral Communication Capability Self‐test (OCCS) was developed. This study also investigates the typology of purchasing managers based on their oral communication capabilities.
Performance of RENA-3 IC with position-sensitive solid-state detectors (2008)

The RENA-3 (R Readout E eadout Electronics for N lectronics Nuclear A uclear Applications) is a multi-channel mixed-signal integrated circuit (IC) developed for the readout of position-sensitive solid-state detectors with excellent energy resolution. We will present results of experiments characterizing its performance as used with a variety of spectroscopy-grade detectors currently available in the industry, notably CZT pixel arrays as well as other detector configurations. The merits of specific RENA-3 design features vis-à-vis different detector applications will also be discussed.
An automated truck platoon for energy saving (2011)

This paper presents an automated truck platoon that has been developed under a national ITS project named Energy ITS. The project, started in 2008, aims at energy saving and global warming prevention with ITS technologies. A platoon of 3 fully-automated trucks currently drives at 80 km/h with the gap of 10 m on a test truck and along an expressway before public use, under not only steady state driving but also lane changing. The lateral control is based on the lane marker detection by the computer vision, and the longitudinal control is based on the gap measurement by 76 GHz radar and lidar in addition to the inter-vehicle communications of 5.8 GHz DSRC. The radar and lidar also work as the obstacle detection. The feature of the technologies is the high reliability, aiming at the near future introduction. Fuel consumption measurement on a test track and along an expressway shows that the fuel can be saved by about 14 %. The evaluation simulation shows that the effectiveness of the platooning with the gap of 10 m when the 40 % penetration in heavy trucks is 2.1 % reduction of CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> along an expressway. The introduction scenario is also discussed.