gebhart/patrepeval huggingface dataset:
Human globin gene expression after gene transfer. (1987)

Human globin genes can be transferred into mouse and human erythroid cells in culture, and can be appropriately expressed at the mRNA level in these cells. A plasmid containing a human beta globin gene is expressed in mouse erythroleukemia cells (MELC), and another containing a human epsilon or gamma gene is expressed in human erythroleukemia (K562) cells. A neomycin resistance (neoR) gene on the plasmids has been used to select for those cells containing the transferred globin genes; this selection may favor the expression of the globin genes by providing chromosomal positions requiring neoR expression. Analyzing clones resistant to G418, a neomycin analogue, demonstrated globin mRNA expression and induction. Retroviral vectors have also been used to transfer and appropriately express human beta genes in MELC. In addition, a plasmid containing a dihydrofolate reductase (DHFR) gene as well as neoR and beta globin genes has been used to amplify and express beta globin mRNA in MELC. These experiments suggest that high level appropriate expression of human beta globin genes is feasible and provides potentially useful approaches to the long-range goal of gene therapy for sickle cell anemia and beta thalassemia.
Physical and Genetic Mapping at the ATA/ATC Locus on Chromosome 11q22-23 (1994)

Genetic heterogeneity in ataxia-telangiectasia (A-T) points to four different genes responsible for this disease. The two major A-T genes, ATA and ATC, were localized by genetic analysis close to each other on chromosome 11q22-23, prompting efforts of positional cloning. Essential steps in positional cloning are long-range cloning of the genomic region of interest, and derivation of highly polymorphic markers that would allow further reduction of the interval carrying the A-T gene. We constructed genomic contigs across the D11S611-D1S424 region harbouring the ATA and ATC genes in yeast artificial chromosome (YAC) vectors. These contigs were used as a fine mapping tool and enabled us to localize along the A-T region, eight microsatellite markers generated randomly by genome mapping centres. In addition, we used specific YAC clones to generate five new microsatellite markers based on polymorphic CA repeats. Recombination mapping based on Israeli A-T families indicates that the ATC gene is distal to the locus D11S1817. Further linkage analysis using these markers is expected to reduce the major A-T locus considerably to a size appropriate for cosmid cloning and identification of transcribed sequences.
Dispersities of Polyesters of Various Diphenols Prepared by Irreversible Polycondensations (2016)

Polyesters of bisphenol-A, bisphenol-P, catechol, and sebacic acid are prepared and different synthetic methods are compared. The diphenols are condensed with sebacoyl chloride either in dichloromethane/pyridine or in refluxing chlorobenzene without HCl-acceptor. Furthermore, bisphenol-A acetate is polycondensed with sebacic acid in bulk. All experiments are worked up so that fractionation is avoided. The extent of cyclization is estimated by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and the molar mass distribution by size exclusion chromatography (SEC). Polycondensations in solution yield larger fractions of cyclics and higher dispersities (up to 11). Polycondensations in bulk give lower fractions of cycles and dispersities from 4.6 to 6.3 for high molar mass polyesters or 2.8 to 3.5 for low molar mass products. Characteristic curves describing the dependence of the dispersity on the initial monomer concentration are elaborated.
Role of AVP in pressor responses during activation of central TxA2/PGH2receptors (1997)

Administration of thromboxane A2/prostaglandin H2 (TxA2/PGH2)-receptor agonist U-46619 (2.86 nmol/kg i.v.) to conscious rats increased mean arterial pressure (MAP) by 17 +/- 2 mmHg (n = 6; P < 0.001) and plasma arginine vasopressin (AVP) by 3.5 +/- 1.1 IU/ml (n = 6; P < 0.001). Ifetroban (TxA2/PGH2 antagonist; intracerebroventricularly) prevented both responses. Intracerebroventricular U-46619 increased MAP in Long-Evans rats (n = 6) more than in AVP-deficient Brattleboro rats. AVP V1-receptor antagonist d(CH2)5Tyr(Me)AVP (3 microg/kg i.v.) blocked 67 +/- 5% and 69 +/- 7% of pressor response to intravenous AVP and intracerebroventricular U-46619, respectively. AVP (10 ng/kg i.v.) increased AVP by 4.7 +/- 0.5 pg/ml, comparable to the increase of 3.5 +/- 1.2 pg/ml with intracerebroventricular U-46619 (2.86 nmol/kg), but the rise in MAP was only one-half as great (+8 +/- 3 mmHg for AVP vs. +17 +/- 2 mmHg for U-46619; P < 0.05). In conclusion, U-46619 raises blood pressure and releases AVP by activating brain receptors. AVP explains approximately one-half of the pressor response.
Incretins and their role in the management of diabetes (2007)

The ability of incretin mimetics to improve glycemic control and reduce body weight is a unique property that fills an important void in the treatment of patients with type 2 diabetes.
Experimental Errors Associated with the Estimation of Thermal Diffusivity from Thermal Process Data (1987)

ABSTRACT An increase in the accuracy and precision of thermal diffusivity (α) was observed when estimated from thermal process data using the analytical heat conduction equation as compared to using the process f h value. Differences in α for the two methods ranged from 2.4 to 3.5%. Correcting for the presence of the thermocouple probe became more important with smaller can sizes. The analytical solution to the heat conduction equation proved to be a very sensitive model with regard to prolonged retort come‐up time and heat conduction along the thermocouple probe.
Comparison of the structural and orientational glass-transition dynamics in ethanol (1998)

The dynamics of the supercooled-liquid and rotator-phase crystal of the same material, ethanol, are compared from measurements of the complex dielectric susceptibility. The relaxation times for the two phases are very close and appear to cross at 96 K, the temperature corresponding both to the structural glass transition of the supercooled liquid and to the orientational glass transition of the rotator phase. Above 96 K, the relaxation time of the supercooled liquid decreases faster than that of the rotator phase, presumably due to the liberation of additional translational degrees of freedom in the former. The dielectric results, coupled with recent specific-heat measurements of the two phases, suggest that the rotational degrees of freedom provide the dominant contribution to structural relaxation near the glass transition, while flow processes provide a smaller contribution.
Coastal Vulnerability Index for Kuwaiti Coast (2021)

Kuwait is investing significantly for the development of coastal infrastructures in many of its coastal areas. It is essential to know the vulnerability of the coast of Kuwait for the future sea level rise and other physically influencing parameters. For this purpose, a detailed study is carried out and the coastal vulnerability index (CVI) is established for the Kuwaiti coast including the coasts of Islands. The CVI is assessed based on the data on coastal geomorphology, historical shoreline change rate, &nbsp;regional coastal slope, mean significant wave height, mean tidal range and the particle size of the sediments along the beach. The total Kuwaiti coastline of 499 km is divided into two groups with a total of 162 coastal cells. The group 1 contains 138 cells and covers the mainland, Boubyan and Failaka Island. The group 2 contains 24 locations and covers Umm Al-Maradim, Qaru and Kubbar Islands. From this study it is found that 5% of the Kuwaiti coastal area in group 1 are very low vulnerable; 34% low vulnerable; 31% moderately vulnerable; 18% highly vulnerable and 12%&nbsp; are very highly vulnerable. Similar results are obtained for group 2 coastal cells. This CVI information on physical vulnerability of the coastal area are carried out based on field data collection for Geomorphology, beach slope, and grain size of the beach soil, numerical model based data on wave height and tidal information and remote sensing based shoreline change detection. The results of this study will be useful for Integrated Coastal Zone Management plan of Kuwaiti coast and for its sustainable coastal infrastructure development.
Use of the Malthus‐AT system to assess the efficacy of permeabilizing agents on the activity of antibacterial agents against Pseudomonas aeruginosa (1998)

The Malthus-AT system provided a satisfactory method for examining the effects of permeabilizing agents on the activity of sub-inhibitory concentrations of antibacterial agents against Pseudomonas aeruginosa G48. Under this system, disodium edetate potentiated the activity of chlorhexidine diacetate (CHA), cetylpyridinium chloride, para-chlorometaxylenol and triclosan. Nitrilotriacetic acid enhanced the activity of some of the antibacterials tested, whereas sodium polyphosphate markedly reduced the efficacy of CHA.
DyBED: An Efficient Algorithm for Updating Betweenness Centrality in Directed Dynamic Graphs (2018)

An important index widely used to analyze social and information networks is betweenness centrality. In this paper, given a dynamic and directed graph G and a vertex r in G, we present the DyBED algorithm that updates the (approximate) betweenness centrality of r, when an update operation (vertex/edge insertion/deletion) occurs in G. Our algorithm first during pre-processing computes two subsets of the vertex set of G, called ΠT(r) and ΠT(r). The Cartesian product of these two sets defines the sample space of our algorithm. In other words, each sample is a pair, whose first element belongs to ΠT(r) and second element belongs to ΠT(r). Then after each update operation, DyBED updates the sets ΠT(r) and ΠT(r), the sampled pairs, the information stored for each sample and accordingly, the betweenness centrality of r. We theoretically and empirically evaluate DyBED and show that it yields significant improvement over existing work. In particular, our extensive experiments reveal that DyBED is orders of magnitude faster than most efficient existing algorithms.
Acetyl-CoA induces transcription of the key G1 cyclin CLN3 to promote entry into the cell division cycle in Saccharomyces cerevisiae (2013)

In budding yeast cells, nutrient repletion induces rapid exit from quiescence and entry into a round of growth and division. The G1 cyclin CLN3 is one of the earliest genes activated in response to nutrient repletion. Subsequent to its activation, hundreds of cell-cycle genes can then be expressed, including the cyclins CLN1/2 and CLB5/6. Although much is known regarding how CLN3 functions to activate downstream targets, the mechanism through which nutrients activate CLN3 transcription in the first place remains poorly understood. Here we show that a central metabolite of glucose catabolism, acetyl-CoA, induces CLN3 transcription by promoting the acetylation of histones present in its regulatory region. Increased rates of acetyl-CoA synthesis enable the Gcn5p-containing Spt-Ada-Gcn5-acetyltransferase transcriptional coactivator complex to catalyze histone acetylation at the CLN3 locus alongside ribosomal and other growth genes to promote entry into the cell division cycle.
Correction: Cardiac ferroportin regulates cellular iron homeostasis and is important for cardiac function (Proceedings of the National Academy of Sciences (2015) 112 (3164-3169) DOI: 10.1073/pnas.1422373112) (2015)

TEST 02 - Elsevier's Scopus, the largest abstract and citation database of peer-reviewed literature. Search and access research from the science, technology, medicine, social sciences and arts and humanities fields.
Application of GPS tracking techniques to orbit determination for TDRS (1993)

In this paper, we evaluate two fundamentally different approaches to TDRS orbit determination utilizing Global Positioning System (GPS) technology and GPS-related techniques. In the first, a GPS flight receiver is deployed on the TDRSS spacecraft. The TDRS ephemerides are determined using direct ranging to the GPS spacecraft, and no ground network is required. In the second approach, the TDRSS spacecraft broadcast a suitable beacon signal, permitting the simultaneous tracking of GPS and TDRSS satellites from a small ground network. Both strategies can be designed to meet future operational requirements for TDRS-2 orbit determination.
Electrochromic Polymer Derived from Oxidized Tetrakis(2-hydroxyphenoxy)Phthalocyaninatocobalt(II) Complex (1997)

Abstract Tetrakis(2-hydroxyphenoxy)phthalocyaninatocobalt(II) complex 3 is synthesized and electrochemically polymerized to give polyphenyleneoxide films containing Co(II) phthalocyanine which exhibit an electrochromic behavior.
Incompatibility of Common Concrete Materials – Influential Factors, Effects, And Prevention (2006)

“Incompatibility” of materials can result in unpredictable concrete set (severely extended or even flash set) and unacceptable strength gain, resulting in rejected concrete or significant finishing, curing, and cracking issues. Such occurrences have become more common with high performance and more complex mixtures. Contributing influences include high temperatures, cement sulfate levels, use of class C fly ash, certain chemical admixture formulations, admixture dosages, and methods used to retard hot-weather concrete. These phenomena have been reproduced and explored with sensitivity studies in laboratory testing using simple mortar cube strength and paste calorimetry methods, shown to be useful for identifying potentially problematic material combinations and conditions. Changing one or more of the key material or mixture characteristics was usually successful in restoring normal behavior. Recommendations are presented for avoiding related field concrete issues.
Le perfectionnisme social et la critique feuerbachienne du personnalisme chrétien de Friedrich Julius Stahl (2017)

La critique feuerbachienne du personnalisme stahlien vise non pas un rejet de toute théorie de la personnalité, mais en propose au contraire une conception intersubjective mettant l’accent sur l’autonomie et la conscience de soi. Le perfectionnisme social de Feuerbach, héritier à la fois de Hegel et Fichte, fonde à ce titre la liberté et la personnalité comme un accomplissement historique progressif résultant de la coordination des activités de chacun au sein d’une société donnée. En cela, sa critique de Stahl n’implique pas seulement un rejet de la monarchie, mais également le projet d’une refondation d’un républicanisme démocratique.
System issues for WDM components (2002)

Two major advantages of wavelength-division-multiplexing (WDM) are: (i) added capacity when transmitting several wavelengths on the same fiber simultaneously, and (ii) added throughput and flexibility in a network when routing of data is based on the wavelength of the signal. This paper will describe some of the limitations which WDM devices place on optical system and network performance and, conversely, some of the specifications which systems require of WDM devices.
Effect of thiourea on polarographic reduction of indium(III) in perchloric acid (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of thiourea on polarographic reduction of indium(III) in perchloric acidKazimierz. Sykut, Grazyna. Dalmata, Barbara. Nowicka, and Jadwiga. SabaCite this: Anal. Chem. 1982, 54, 3, 600–602Publication Date (Print):March 1, 1982Publication History Published online1 May 2002Published inissue 1 March 1982https://pubs.acs.org/doi/10.1021/ac00240a063https://doi.org/10.1021/ac00240a063research-articleACS PublicationsRequest reuse permissionsArticle Views60Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
System Metrics of Brillouin Integrated RF Photonic Filters (2019)

In this paper, we theoretically and experimentally compare and optimise the noise figure and link gain of Brillouin-based integrated microwave photonic bandpass filters synthesised using different modulation schemes and switching between Brillouin loss and gain.
The circadian clock modulates susceptibility of mice to ventilator-induced lung injury (2018)

For patients with acute respiratory distress syndrome (ARDS) mechanical ventilation (MV) is a life-saving therapy, but it can also exacerbate pre-existing lung injury. This process, termed as ventilator-induced lung injury (VILI), is characterized by increased pulmonary permeability, inflammation and activation of the innate immune system.
The botulinum neurotoxin: a deadly protease with applications to human medicine (2009)

https://scholarlycommons.pacific.edu/cop-facbooks/1202/thumbnail.jpg
Kefir protective effects against nicotine cessation-induced anxiety and cognition impairments in rats (2014)

This study revealed that Kefir had a potential effect on the treatment of nicotine cessation-induced depression, anxiety and cognition impairment in the animal model. Kefir may be useful for adjunct therapy for nicotine abandonment treatment protocols.
1-Diazo-2-propene (2001)

[2032-04-4] C3H4N2 (MW 68.08) InChI = 1S/C3H4N2/c1-2-3-5-4/h2-3H,1H2 InChIKey = AJYIGJXFGZQYHI-UHFFFAOYSA-N (reagent for the cyclopropanation of alkenes) Alternate Name: vinyldiazomethane. Solubility: vinyldiazomethane is most commonly prepared and used as a solution in ether or THF, or as an alcoholic solution in either of those solvents. Analysis of Reagent Purity: the amount of active diazo compound contained in a solution has been determined by measuring the volume of nitrogen evolved upon reaction of an aliquot of the diazo compound with an excess of p-nitrobenzoic acid in THF.3 Preparative Methods: the reagent is most commonly prepared by the action of base on various N-allyl-N-nitroso derivatives. Thus ethyl-N-allyl-N-nitrosocarbamate has been used,1 as has N-allyl-N-nitrosourea.2 The reagent is light sensitive and should therefore be shielded from light during its preparation. It undergoes isomerization to pyrazole at rt1c and is therefore prepared immediately prior to use, and not usually stored for any length of time. Furthermore, it is potentially explosive and for this reason the chemist is advised to follow carefully the exact procedure given for a particular preparation and perform all operations behind a blast shield in a well ventilated fume hood. Handling, Storage, and Precautions: in addition to the previously mentioned explosion hazard which this reagent presents, there are health related hazards that must also be considered. Diazo compounds, as a class, are toxic and irritating and can be sensitizers. They must be used in a well ventilated fume hood and not come in contact with skin. For a description of the hazards associated with this class of compounds, see the handling and storage precautions listed for Diazomethane.
Emerging roles and underlying molecular mechanisms of DNAJB6 in cancer (2016)

DNAJB6 also known as mammalian relative of DnaJ (MRJ) encodes a highly conserved member of the DnaJ/Hsp40 family of co-chaperone proteins that function with Hsp70 chaperones. DNAJB6 is widely expressed in all tissues, with higher expression levels detected in the brain. DNAJB6 is involved in diverse cellular functions ranging from murine placental development, reducing the formation and toxicity of mis-folded protein aggregates, to self-renewal of neural stem cells. Involvement of DNAJB6 is implicated in multiple pathologies such as Huntington's disease, Parkinson's diseases, limb-girdle muscular dystrophy, cardiomyocyte hypertrophy and cancer. This review summarizes the important involvement of the spliced isoforms of DNAJB6 in various pathologies with a specific focus on the emerging roles of human DNAJB6 in cancer and the underlying molecular mechanisms.
Dynamic Behavior of Reciprocating Plunger Pump Discharge Valve Based on Fluid Structure Interaction and Experimental Analysis (2015)

The influence of spring stiffness and valve quality on the motion behaviors of reciprocating plunger pump discharge valves was investigated by fluid structure interaction (FSI) simulation and experimental analysis. The mathematical model of the discharge valve motion of a 2000-fracturing pump was developed and the discrete differential equations were solved according to FSI and results obtained by ANDINA software. Results indicate that spring stiffness influences the maximum lift, the opening resistance and shut-off lag angle, as well as the fluid velocity of the clearance, the impact stress and the volume efficiency of the pump valve in relation to the valve quality. An optimal spring stiffness parameter of 14.6 N/mm was obtained, and the volumetric efficiency of the pumping valve increased by 4‰ in comparison to results obtained with the original spring stiffness of 10.09N/mm. The experimental results indicated that the mathematical model and FSI method could provide an effective approach for the subsequent improvement of valve reliability, volumetric efficiency and lifespan.
High abundance synovial fluid proteome: distinct profiles in health and osteoarthritis (2007)

The development of increasingly high-throughput and sensitive mass spectroscopy-based proteomic techniques provides new opportunities to examine the physiology and pathophysiology of many biologic fluids and tissues. The purpose of this study was to determine protein expression profiles of high-abundance synovial fluid (SF) proteins in health and in the prevalent joint disease osteoarthritis (OA). A cross-sectional study of 62 patients with early OA (n = 21), patients with late OA (n = 21), and control individuals (n = 20) was conducted. SF proteins were separated by using one-dimensional PAGE, and the in-gel digested proteins were analyzed by electrospray ionization tandem mass spectrometry. A total of 362 spots were examined and 135 high-abundance SF proteins were identified as being expressed across all three study cohorts. A total of 135 SF proteins were identified. Eighteen proteins were found to be significantly differentially expressed between control individuals and OA patients. Two subsets of OA that are not dependent on disease duration were identified using unsupervised analysis of the data. Several novel SF proteins were also identified. Our analyses demonstrate no disease duration-dependent differences in abundant protein composition of SF in OA, and we clearly identified two previously unappreciated yet distinct subsets of protein profiles in this disease cohort. Additionally, our findings reveal novel abundant protein species in healthy SF whose functional contribution to SF physiology was not previously recognized. Finally, our studies identify candidate biomarkers for OA with potential for use as highly sensitive and specific tests for diagnostic purposes or for evaluating therapeutic response.