gebhart/patrepeval huggingface dataset:
The histological structure of dog knee menisci with comments on its possible significance (1976)

Knee-joint menisci are poorly understood terminologically, structurally and functionally in spite of their almost universal occurrence in mammals and their considerable clinical significance in man. A study was therefore undertaken of dog knee menisci utilizing several histological techniques. Terminologically, it is proposed that the part of the meniscus extending between the anterior and posterior horns and exclusive of them be called the meniscal "body." Structurally, the horns and body differ in anumber of ways. The horns are oval in cross section, the body triangular. Hyalinized areas are much more frequent in the body than in the horns. The collagen of the horns is organized into discrete bundles that are separated from one another by loose connective tissue septa, while that of the body is arranged in a "herringbone" pattern; no septa are present in the body. Finally, the meniscal horns are richly supplied with blood vessels are nerves (including large myelinated fibers which apparently terminate in the horns) while the body is almost completely devoid of blood vessels and nerves. Functionally, it is hypothesized that knee-joint menisci may serve important sensory functions.
GPCRs show widespread differential mRNA expression and frequent mutation and copy number variation in solid tumors (2019)

G protein-coupled receptors (GPCRs) are the most widely targeted gene family for Food and Drug Administration (FDA)-approved drugs. To assess possible roles for GPCRs in cancer, we analyzed The Cancer Genome Atlas (TCGA) data for mRNA expression, mutations, and copy number variation (CNV) in 20 categories and 45 subtypes of solid tumors and quantified differential expression (DE) of GPCRs by comparing tumors against normal tissue from the Gene Tissue Expression Project (GTEx) database. GPCRs are overrepresented among coding genes with elevated expression in solid tumors. This analysis reveals that most tumor types differentially express >50 GPCRs, including many targets for approved drugs, hitherto largely unrecognized as targets of interest in cancer. GPCR mRNA signatures characterize specific tumor types and correlate with expression of cancer-related pathways. Tumor GPCR mRNA signatures have prognostic relevance for survival and correlate with expression of numerous cancer-related genes and pathways. GPCR expression in tumors is largely independent of staging, grading, metastasis, and/or driver mutations. GPCRs expressed in cancer cell lines largely parallel GPCR expression in tumors. Certain GPCRs are frequently mutated and appear to be hotspots, serving as bellwethers of accumulated genomic damage. CNV of GPCRs is common but does not generally correlate with mRNA expression. Our results suggest a previously underappreciated role for GPCRs in cancer, perhaps as functional oncogenes, biomarkers, surface antigens, and pharmacological targets.
Application of the Network Simulation Method to Flat Dies with Inverted Prelands (2013)

We present a systematic approach based on Kirchhoff’s laws that uses tensor algebra and analytical formulae obtained from constitutive equations for the analysis of flow resistance networks. The flow of a Carreau fluid in a flat die with inverted preland is analyzed in terms of mass flow, velocity, and residence time distributions. Viscous heating is taken into account by using an analytical model which depends on the Cameron number of the flow at different positions. Further, we report the results of a three-dimensional simulation, using the commercially available ANSYS Polyflow software, which are in good agreement with our numerical study.
A neomycin‐resistant cell line for improved production of monoclonal antibodies to cell surface antigens (1985)

Overgrowth of hybridomas by proliferating spleen T cells often hinders the production of monoclonal antibodies to certain antigens. To overcome this problem we derived neomycin-resistant fusion partner SP2 neoR.1 by transfection of SP2/0-Ag14 cells with plasmid pSVTK neo beta. Hybridomas obtained with SP2 neoR.1 grew optimally in the presence of the neomycin analog G418 at concentrations which blocked the proliferative response of T cells to mitogenic stimuli. The advantage of using SP2 neoR.1 and G418 under conditions where spleen cell proliferation occurs after fusion was demonstrated with hybridomas derived from a rat immunized with mouse helper T cell lines.
Non-del(5q) myelodysplastic syndromes–associated loci detected by SNP-array genome-wide association meta-analysis (2019)

Myelodysplastic syndromes (MDS) are hematopoietic stem cell malignancies. Known predisposing factors to adult MDS include rare germline mutations, cytotoxic therapy, age-related clonal hematopoiesis, and autoimmune or chronic inflammatory disorders. To date, no published studies characterizing MDS-associated germline susceptibility polymorphisms exist. We performed a genome-wide association study of 2 sample sets (555 MDS cases vs 2964 control subjects; 352 MDS cases vs 2640 control subjects) in non-del(5q) MDS cases of European genomic ancestry. Meta-analysis identified 8 MDS-associated loci at 1q31.1 (PLA2G4A), 3p14.1 (FAM19A4), 5q21.3 (EFNA5), 6p21.33, 10q23.1 (GRID1), 12q24.32, 15q26.1, and 20q13.12 (EYA2) that approached genome-wide significance. Gene expression for 5 loci that mapped within or near genes was significantly upregulated in MDS bone marrow cells compared with those of control subjects (P < .01). Higher PLA2G4A expression and lower EYA2 expression were associated with poorer overall survival (P = .039 and P = .037, respectively). Higher PLA2G4A expression is associated with mutations in NRAS (P < .001), RUNX1 (P = .012), ASXL1 (P = .007), and EZH2 (P = .038), all of which are known to contribute to MDS development. EYA2 expression was an independently favorable risk factor irrespective of age, sex, and Revised International Scoring System score (relative risk, 0.67; P = .048). Notably, these genes have regulatory roles in innate immunity, a critical driver of MDS pathogenesis. EYA2 overexpression induced innate immune activation, whereas EYA2 inhibition restored colony-forming potential in primary MDS cells indicative of hematopoietic restoration and possible clinical relevance. In conclusion, among 8 suggestive MDS-associated loci, 5 map to genes upregulated in MDS with functional roles in innate immunity and potential biological relevance to MDS.
Angiotensin II stimulates nitric oxide production in pulmonary artery endothelium via the type 2 receptor (2004)

We previously reported that angiotensin II stimulates an increase in nitric oxide production in pulmonary artery endothelial cells. The aims of this study were to determine which receptor subtype mediates the angiotensin II-dependent increase in nitric oxide production and to investigate the roles of the angiotensin type 1 and type 2 receptors in modulating angiotensin II-dependent vasoconstriction in pulmonary arteries. Pulmonary artery endothelial cells express both angiotensin II type 1 and type 2 receptors as assessed by RT-PCR, Western blot analysis, and flow cytometry. Treatment of the endothelial cells with PD-123319, a type 2 receptor antagonist, prevented the angiotensin II-dependent increase in nitric oxide synthase mRNA, protein levels, and nitric oxide production. In contrast, the type 1 receptor antagonist losartan enhanced nitric oxide synthase mRNA levels, protein expression, and nitric oxide production. Pretreatment of the endothelial cells with either PD-123319 or an anti-angiotensin II antibody prevented this losartan enhancement of nitric oxide production. Angiotensin II-dependent enhanced hypoxic contractions in pulmonary arteries were blocked by the type 1 receptor antagonist candesartan; however, PD-123319 enhanced hypoxic contractions in angiotensin II-treated endothelium-intact vessels. These data demonstrate that angiotensin II stimulates an increase in nitric oxide synthase mRNA, protein expression, and nitric oxide production via the type 2 receptor, whereas signaling via the type 1 receptor negatively regulates nitric oxide production in the pulmonary endothelium. This endothelial, type 2 receptor-dependent increase in nitric oxide may serve to counterbalance the angiotensin II-dependent vasoconstriction in smooth muscle cells, ultimately regulating pulmonary vascular tone.
A New Type of Peroxisomal Acyl-Coenzyme A Synthetase from Arabidopsis thaliana Has the Catalytic Capacity to Activate Biosynthetic Precursors of Jasmonic Acid (2005)

Arabidopsis thaliana contains a large number of genes that encode carboxylic acid-activating enzymes, including nine long-chain fatty acyl-CoA synthetases, four 4-coumarate:CoA ligases (4CL), and 25 4CL-like proteins of unknown biochemical function. Because of their high structural and sequence similarity with bona fide 4CLs and their highly hydrophobic putative substrate-binding pockets, the 4CL-like proteins At4g05160 and At5g63380 were selected for detailed analysis. Following heterologous expression, the purified proteins were subjected to a large scale screen to identify their preferred in vitro substrates. This study uncovered a significant activity of At4g05160 with medium-chain fatty acids, medium-chain fatty acids carrying a phenyl substitution, long-chain fatty acids, as well as the jasmonic acid precursors 12-oxo-phytodienoic acid and 3-oxo-2-(2'-pentenyl)-cyclopentane-1-hexanoic acid. The closest homolog of At4g05160, namely At5g63380, showed high activity with long-chain fatty acids and 12-oxo-phytodienoic acid, the latter representing the most efficiently converted substrate. By using fluorescent-tagged variants, we demonstrated that both 4CL-like proteins are targeted to leaf peroxisomes. Collectively, these data demonstrate that At4g05160 and At5g63380 have the capacity to contribute to jasmonic acid biosynthesis by initiating the beta-oxidative chain shortening of its precursors.
Low supply voltage fully integrated CMOS VCO with three terminals spiral inductor (1999)

The very low phase noise and very low supply voltage 1.4 GHz CMOS VCO has been realized by 0.25 µm standard CMOS technology without any trimming and any tuning. The phase noise characteristics are achieved approximately -100 dBc/Hz at 100kHz offset with 1.3V power supply. To reduce supply voltage, the amplifier topology is consisted one pair of PMOS differential stage with special shapes of coil structure which is three terminals on chip spiral inductor. To reduce a flicker noise component coming from current source of the amplifier, the very large current source transistor is adapted.
A study of internet instant messaging and chat protocols (2006)

Instant messaging (IM) and network chat communication have seen an enormous rise in popularity over the last several years. However, since many of these systems are proprietary, little has been described about the network technology behind them. This analysis helps bridge this gap by providing an overview of the available features, functions, system architectures, and protocol specifications of the three most popular network IM protocols: AOL Instant Messenger, Yahoo! Messenger, and Microsoft Messenger. We describe common features across these systems and highlight distinctions between them. Where possible, we discuss the advantages and disadvantages of different technical approaches used in these systems to support different features and functions. We also briefly discuss ongoing efforts to standardize IM and chat-based protocols in IETF and other standards bodies.
Chromatography and Electrochromatography (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChromatography and ElectrochromatographyH. H. Strain and T. R. SatoCite this: Anal. Chem. 1956, 28, 4, 687–694Publication Date (Print):April 1, 1956Publication History Published online1 May 2002Published inissue 1 April 1956https://pubs.acs.org/doi/10.1021/ac60112a018https://doi.org/10.1021/ac60112a018research-articleACS PublicationsRequest reuse permissionsArticle Views58Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Adaptive Logging for Distributed In-memory Databases (2015)

A new type of logs, the command log, is being employed to replace the traditional data log (e.g., ARIES log) in the in-memory databases. Instead of recording how the tuples are updated, a command log only tracks the transactions being executed, thereby effectively reducing the size of the log and improving the performance. Command logging on the other hand increases the cost of recovery, because all the transactions in the log after the last checkpoint must be completely redone in case of a failure. In this paper, we first extend the command logging technique to a distributed environment, where all the nodes can perform recovery in parallel. We then propose an adaptive logging approach by combining data logging and command logging. The percentage of data logging versus command logging becomes an optimization between the performance of transaction processing and recovery to suit different OLTP applications. Our experimental study compares the performance of our proposed adaptive logging, ARIES-style data logging and command logging on top of H-Store. The results show that adaptive logging can achieve a 10x boost for recovery and a transaction throughput that is comparable to that of command logging.
Nekara: Generalized Concurrency Testing (2021)

Testing concurrent systems remains an uncomfortable problem for developers. The common industrial practice is to stress-test a system against large workloads, with the hope of triggering enough corner-case interleavings that reveal bugs. However, stress testing is often inefficient and its ability to get coverage of interleavings is unclear. In reaction, the research community has proposed the idea of systematic testing, where a tool takes over the scheduling of concurrent actions so that it can perform an algorithmic search over the space of interleavings.We present an experience paper on the application of systematic testing to several case studies. We separate the algorithmic advancements in prior work (on searching the large space of interleavings) from the engineering of their tools. The latter was unsatisfactory; often the tools were limited to a small domain, hard to maintain, and hard to extend to other domains. We designed Nekara, an open-source cross-platform library for easily building custom systematic testing solutions.We show that (1) Nekara can effectively encapsulate state-of-the-art exploration algorithms by evaluating on prior bench-marks, and (2) Nekara can be applied to a wide variety of scenarios, including existing open-source systems as well as production distributed services of Microsoft Azure. Nekara was easy to use, improved testing, and found multiple new bugs.
The influence of vascularization of transplanted processed allograft nerve on return of motor function in rats (2014)

Processed nerve allografts have become an alternative to repair segmental nerve defects, with results comparable with autografts regarding sensory recovery; however, they have failed to reproduce comparable motor recovery. The purpose of this study was to determine how revascularizaton of processed nerve allograft would affect motor recovery. Eighty-eight rats were divided in four groups of 22 animals each. A unilateral 10-mm sciatic nerve defect was repaired with allograft (group I), allograft wrapped with silicone conduit (group II), allograft augmented with vascular endothelial growth factor (group III), or autograft (group IV). Eight animals from each group were sacrificed at 3 days, and the remaining animals at 16 weeks. Revascularization was evaluated by measuring the graft capillary density at 3 days and 16 weeks. Measurements of ankle contracture, compound muscle action potential, tibialis anterior muscle weight and force, and nerve histomorphometry were performed at 16 weeks. All results were normalized to the contralateral side. The results of capillary density at 3 days were 0.99% ± 1.3% for group I, 0.33% ± 0.6% for group II, 0.05% ± 0.1% for group III, and 75.6% ± 45.7% for group IV. At 16 weeks, the results were 69.9% ± 22.4% for group I, 37.0% ± 16.6% for group II, 84.6% ± 46.6% for group III, and 108.3% ± 46.8% for group IV. The results of muscle force were 47.5% ± 14.4% for group I, 21.7% ± 13.5% for group II, 47.1% ± 7.9% for group III, and 54.4% ± 10.6% for group IV. The use of vascular endothelial growth factor in the fashion used in this study improved neither the nerve allograft short-term revascularization nor the functional motor recovery after 16 weeks. Blocking allograft vascularization from surrounding tissues was detrimental for motor recovery. The processed nerve allografts used in this study showed similar functional motor recovery compared with that of the autograft.
(Keynote) Colloidal Quantum Shells: An Emerging Class of 2D Semiconductors (2022)

Low-dimensional colloidal semiconductors are inexpensive to process and offer a broad spectrum of attractive quantum-mechanical properties. The notorious problem of these nanostructures lies in their limited performance under high energetic loads, when more than one exciton per particle is created. Multiple excitons undergo fast Auger recombination, causing efficiently roll-off in energy-intensive applications, including high-brightness LEDs, X-ray scintillators, and solar cells. This presentation will discuss an emerging type of low-dimensional semiconductors, known as colloidal quantum shells that allow avoiding such multi-exciton (MX) energy losses. The geometry of quantum shells benefits from the spatial separation of multiple excitons, which leads to extraordinary improvements to MX lifetimes and MX quantum yield. We will compare quantum shells with other nanoscale geometries in three categories: Auger suppression, multi-exciton level structure, and solution-processed film conductivity, highlighting the potential benefits quantum shells can contribute to various applications. Theoretical analysis discussing the role of the quantum shell geometry in suppressing Auger recombination will also be presented.
Optimal projection to improve parametric importance sampling in high\n dimension (2021)

In this paper we propose a dimension-reduction strategy in order to improve\nthe performance of importance sampling in high dimension. The idea is to\nestimate variance terms in a small number of suitably chosen directions. We\nfirst prove that the optimal directions, i.e., the ones that minimize the\nKullback--Leibler divergence with the optimal auxiliary density, are the\neigenvectors associated to extreme (small or large) eigenvalues of the optimal\ncovariance matrix. We then perform extensive numerical experiments that show\nthat as dimension increases, these directions give estimations which are very\nclose to optimal. Moreover, we show that the estimation remains accurate even\nwhen a simple empirical estimator of the covariance matrix is used to estimate\nthese directions. These theoretical and numerical results open the way for\ndifferent generalizations, in particular the incorporation of such ideas in\nadaptive importance sampling schemes.\n
Higher-Order Filtering Attenuator Design Considerations for Filter Shape Optimization (2023)

Filtering attenuators (filtenuators) combine the frequency selectivity of filters with the controlled loss of an attenuator in a single component. Often, the filtenuator is made of three resonators or less to minimize the complexity of the design and the resistive π-network distribution is straightforward. However, the resistive network distribution is less clear for high-order filtenuator designs. This paper presents an alternative method of loading a filtenuator that has more than three resonators and is compared to the traditional π-network method of loading a resonating circuit. A detailed analysis of the resistive network distribution is conducted to optimize the filter shape using a fifth-order Chebyshev bandpass filter topology. A 900 MHz fifth-order filtenuator with 6.5 dB of passband loss is designed using the optimized distribution method, and the measured results are compared to simulation.
Oxime-assisted Acetylcholinesterase Catalytic Scavengers of Organophosphates That Resist Aging (2011)

The cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase, are primary targets of organophosphates (OPs). Exposure to OPs can lead to serious cardiovascular complications, respiratory compromise, and death. Current therapy to combat OP poisoning involves an oxime reactivator (2-PAM, obidoxime, TMB4, or HI-6) combined with atropine and on occasion an anticonvulsant. Butyrylcholinesterase, administered in the plasma compartment as a bio-scavenger, has also shown efficacy but is limited by its strict stoichiometric scavenging, slow reactivation, and a propensity for aging. Here, we characterize 10 human (h) AChE mutants that, when coupled with an oxime, give rise to catalytic reactivation and aging resistance of the soman conjugate. With the most efficient human AChE mutant Y337A/F338A, we show enhanced reactivation rates for several OP-hAChE conjugates compared with wild-type hAChE when reactivated with HI-6 (1-(2'-hydroxyiminomethyl-1'-pyridinium)-3-(4'-carbamoyl-1-pyridinium)). In addition, we interrogated an 840-member novel oxime library for reactivation of Y337A/F338A hAChE-OP conjugates to delineate the most efficient oxime-mutant enzyme pairs for catalytic bio-scavenging. Combining the increased accessibility of the Y337A mutation to oximes within the space-impacted active center gorge with the aging resistance of the F338A mutation provides increased substrate diversity in scavenging potential for aging-prone alkyl phosphate inhibitors.
[Bilateral optic neuropathy in acute methanol intoxication. A case report]. (2001)

We report a case of methanol blindness. Ophthalmoscopic examination disclosed swelling in the disc margins extending along the adjacent retinal nerve fiber layer. Although this optic neuropathy is now rare, prompt diagnosis and proper treatment in the acute phase can dramatically improve the prognosis.