gebhart/patrepeval huggingface dataset:
RNase E is required for the maturation of ssrA RNA and normal ssrA RNA peptide-tagging activity (1999)

During recent studies of ribonucleolytic "degradosome" complexes of Escherichia coli, we found that degradosomes contain certain RNAs as well as RNase E and other protein components. One of these RNAs is ssrA (for small stable RNA) RNA (also known as tm RNA or 10Sa RNA), which functions as both a tRNA and mRNA to tag the C-terminal ends of truncated proteins with a short peptide and target them for degradation. Here, we show that mature 363-nt ssrA RNA is generated by RNase E cleavage at the CCA-3' terminus of a 457-nt ssrA RNA precursor and that interference with this cleavage in vivo leads to accumulation of the precursor and blockage of SsrA-mediated proteolysis. These results demonstrate that RNase E is required to produce mature ssrA RNA and for normal ssrA RNA peptide-tagging activity. Our findings indicate that RNase E, an enzyme already known to have a central role in RNA processing and decay in E. coli, also has the previously unsuspected ability to affect protein degradation through its role in maturation of the 3' end of ssrA RNA.
WIDE-ANGLE FUNDUS IMAGING THROUGH THE BOSTON KERATOPROSTHESIS (2013)

Wide-angle fundus imaging through the keratoprosthesis is possible, and all three cameras performed similarly. The good quality of pictures obtained with the noncontact Optos, as well as its ease of use, comfort, and safety make it a preferred choice. Optos complements B-scan in the examination of the peripheral retina through the keratoprosthesis, and it may even be superior in certain settings.
Evaluation of Traditional Chinese Medicine Herbs in Oncology Clinical Trials (2019)

Despite wide empirical use and demand for traditional Chinese medicine (TCM) herbs worldwide, high-quality clinical trials of TCM herbs in oncology are limited. We developed recommendations for rigorous clinical trials to evaluate their safety and efficacy for oncology patients. To accomplish this goal, the TCM & Cancer Research Committee of the Chinese Pharmaceutical Association convened a working group of oncologists, TCM experts, clinical researchers, biostatisticians, and industry/government representatives to develop principles and approaches for TCM cancer drug clinical trials. They identified 2 categories of herbal drugs based on therapeutic intent: survival improvement and symptom management. The working group also emphasized the need to adopt international standards when appropriate for TCM herbal drug approval and to develop methodology to standardize diagnostic criteria, treatment approaches, and outcome measures according to defining TCM characteristics. These recommendations aim to improve study design and methods to inform rigorous investigation of TCM herbs in patients with cancer.
Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome (2023)

Copy number variations (CNVs) are associated with psychiatric and neurodevelopmental disorders (NDDs), and most, including the recurrent 15q13.3 microdeletion disorder, have unknown disease mechanisms. We used a heterozygous 15q13.3 microdeletion mouse model and patient iPSC-derived neurons to reveal developmental defects in neuronal maturation and network activity. To identify the underlying molecular dysfunction, we developed a neuron-specific proximity-labeling proteomics (BioID2) pipeline, combined with patient mutations, to target the 15q13.3 CNV genetic driver OTUD7A. OTUD7A is an emerging independent NDD risk gene with no known function in the brain, but has putative deubiquitinase function. The OTUD7A protein-protein interaction network included synaptic, axonal, and cytoskeletal proteins and was enriched for ASD and epilepsy risk genes (Ank3, Ank2, SPTAN1, SPTBN1). The interactions between OTUD7A and Ankyrin-G (Ank3) and Ankyrin-B (Ank2) were disrupted by an epilepsy-associated OTUD7A L233F variant. Further investigation of Ankyrin-G in mouse and human 15q13.3 microdeletion and OTUD7A<sup>L233F/L233F</sup> models revealed protein instability, increased polyubiquitination, and decreased levels in the axon initial segment, while structured illumination microscopy identified reduced Ankyrin-G nanodomains in dendritic spines. Functional analysis of human 15q13.3 microdeletion and OTUD7A<sup>L233F/L233F</sup> models revealed shared and distinct impairments to axonal growth and intrinsic excitability. Importantly, restoring OTUD7A or Ankyrin-G expression in 15q13.3 microdeletion neurons led to a reversal of abnormalities. These data reveal a critical OTUD7A-Ankyrin pathway in neuronal development, which is impaired in the 15q13.3 microdeletion syndrome, leading to neuronal dysfunction. Furthermore, our study highlights the utility of targeting CNV genes using cell type-specific proteomics to identify shared and unexplored disease mechanisms across NDDs.
The Diverse Reactivity of Peroxy Ferric Porphyrin Complexes of Electron−Rich and Electron−Poor Porphyrins J. Am. Chem. Soc. 1996, 118, 2008 (1996)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTThe Diverse Reactivity of Peroxy Ferric Porphyrin Complexes of Electron−Rich and Electron−Poor Porphyrins J. Am. Chem. Soc. 1996, 118, 2008Matthias Selke, Marlene F. Sisemore, and Joan Selverstone ValentineCite this: J. Am. Chem. Soc. 1996, 118, 46, 11692Publication Date (Web):November 20, 1996Publication History Published online20 November 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja965452jhttps://doi.org/10.1021/ja965452jcorrectionACS PublicationsCopyright © 1996 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views305Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (67 KB) Get e-Alertsclose Get e-Alerts
Cooperative Group Key Generation Using IR-UWB Multipath Channels (2015)

In wireless decentralized networks, nodes physically close to each other can take advantage of the shared wireless medium to generate common secrets or keys and enhance the security of communications. Previous studies already provided models, algorithms, and proofs of concept to exploit the radio physical layer and generate symmetric keys over single links. Some of these models have been adapted to cooperative scenarios involving several nodes, either to reinforce the generated pair-wise keys or to issue a common group key (i.e., shared by more than two nodes). In this study, we focus on key agreement from Impulse Radio - Ultra Wideband (IR-UWB) multipath channels according to the source model. We propose a new method to generate group keys within cooperative scenarios, while exploiting all the available physical links in a full mesh topology and reducing over-the-air traffic. The main idea consists in adjusting the IR-UWB signals usually transmitted for channel probing so that receiving nodes have access to non-observable channels corresponding to their non-adjacent links. We describe an example where the transmitted signal is optimized using a least squares minimization routine with prior information.
Tuneable separation in elastomeric microfluidics devices (2008)

We describe how the elastomeric properties of PDMS (polydimethylsiloxane) can be utilised to achieve tuneable particle separation in Deterministic Lateral Displacement devices via strain controlled alteration of inter-obstacle distances, a development that opens up new avenues toward more effective separation of particles in microfluidics devices.
Polyamorphism in aluminate liquids (2003)

Supercooled liquids in the Y2O3–Al2O3 system undergo a liquid–liquid phase transition between a high-temperature, high-density amorphous polymorph (HDA form), and one with lower density that is stable at lower temperature (LDA form). The two amorphous polymorphs have the same chemical composition, but they differ in their density (∼4% density difference), and in their heat content (enthalpy) and entropy determined by calorimetry. Here we present new results of structural studies using neutron and high-energy x-ray diffraction to study the structural differences between high-density amorphous (HDA) and low-density amorphous (LDA) polyamorphs. The combined data sets show no large differences in the average nearest-neighbour Al–O or Y–O bond lengths or coordination numbers between the low-and high-density liquids. However, the data indicate that changes occur among the packing geometries and clustering of the Al–O and Y–O coordination polyhedra, i.e., within the second-nearest-neighbour shell defined by the metal–metal (i.e., Y–Y, Y–Al and Al–Al) interactions. Polarizable ion molecular dynamics simulations of Y2O3–Al2O3 liquids are used to help interpret the pair-correlation functions obtained from x-ray and neutron scattering data. Unexpectedly large density fluctuations are observed to occur during the simulation, that are interpreted as due to dynamic sampling of high-and low-density configurations within the single-phase liquid at temperatures above the critical point or phase transition line. Calculated partial radial distribution functions indicate that the primary differences between HDA and LDA configurations occur among the Y–Y correlations.
Antimicrobial Susceptibility Patterns and Staphylococcal Cassette Chromosome mec Types of, as Well as Panton-Valentine Leukocidin Occurrence among, Methicillin-Resistant Staphylococcus aureus Isolates from Children and Adults in Middle Tennessee (2006)

Antimicrobial susceptibility patterns, Panton-Valentine leukocidin (PVL) occurrence, and staphylococcal cassette chromosome mec (SCCmec) types in methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from children and adults at Vanderbilt University Medical Center during a 12-month period were evaluated. A total of 1,315 MRSA isolates were collected, of which 748 (36.7%) were recovered from children. Among all isolates, 448 (34.1%) were SCCmec-II, and 847 (64.4%) were SCCmec-IV. More SCCmec-IV isolates were recovered from children than SCCmec-II isolates (424 [50.1%] versus 50 [11.2%]; odds ration [OR]=7.98; P<0.000001). The PVL gene was detected in 93.6% of SCCmec-IV isolates, in contrast to 0.2% in SCCmec-II isolates. Within SCCmec-IV isolates, a statistically higher PVL occurrence was noticed in children (98.1%) than in adults (89.1%) (OR=6.34; P<0.000001). Overall, SCCmec-II strains showed greater resistance than SCCmec-IV strains to clindamycin, erythromycin, levofloxacin, gentamicin, rifampin, minocycline, and trimethoprim-sulfamethoxazole. Both SCCmec-II and SCCmec-IV strains recovered from adults were more resistant to these antibiotics than those recovered from children. SCCmec-II strains were predominantly recovered from the respiratory tract, whereas SCCmec-IV strains were predominantly recovered from skin, soft tissue, abscesses, and surgical wounds. These data indicate that SCCmec-IV MRSA isolates frequently infect children in middle Tennessee and are likely to harbor the PVL gene.
Dispensasi Nikah Bagi Anak di Bawah Umur Tanpa Persetujuan Wali Perspektif Yuridis (2023)

Issue of underage marriage comes into discussion and requires a very deep study, especially by judges who will provide the fairest decision. This research is a field research questions what the basic consideration of the judge granting a dispensation of marriage filed by minors without the consent of the guardian, in ruling No. 0067/Pdt.P/2012/PA.Pas and how the juridical analysis about it. The result of the research concludes that the judge granted dispensation of marriage without permission of her guardian. The reason are that the marriage is a very urgent to be done because the bride has already been pregnant 6 months. In order not to cause greater damage, the judge granted the request. In relation with the status of applicant, who is still minor, also follows the emergency consideration. Therefore, the applicant was declared to be the subject of law. While the decision does not comply with the provision of Article 7 paragraph (2) Law No. 1 1974 and PMA no. 3 of 1975 concerning with the permissibility of proposing dispensation of marriage to Religious Court that has been designated by parent. And the condition of applicant is not legal competent, this will lead to a formal letter of request becomes disabled due to an error in persona and defect letter. [Persoalan pernikahan di bawah umur menjadi pembahasan tersendiri yang membutuhkan kajian yang sangat mendalam, terutama oleh para hakim yang akan memberikan putusan yang seadil-adilnya. Penelitian ini adalah penelitian lapangan yang mempertanyakan apa dasar pertimbangan hakim dalam mengabulkan permohonan dispensasi nikah yang diajukan oleh anak di bawah umur tanpa izin dari wali, dalam putusan No. 0067/Pdt.P/2012/PA.Pas dan bagaimana analisis yuridisnya. Hasil penelitian menyimpulkan bahwa majelis hakim mengabulkan permohonan dispensasi nikah tanpa persetujuan wali karena majelis hakim menilai perkawinan tersebut sudah sangat mendesak untuk dilakukan karena calon istri tengah hamil 6 bulan agar tidak menimbulkan kerusakan yang lebih besar majelis hakim mengabulkan permohonan tersebut, dan mengenai status pemohon yang masih di bawah umur juga mengikuti pertimbangan darurat tersebut oleh karena itu pemohon dinyatakan sah menjadi subyek hukum. Sedangkan putusan tersebut tidak memenuhi ketentuan pasal 7 ayat(2) undang undang no. 1 tahun 1974 dan PMA no. 3 tahun 1975 tentang kebolehan mengajukan dispensasi nikah ke pengadilan agama yang telah ditunjuk orang tua. Dan pemohon masih belum cakap hukum, hal ini akan menimbulkan surat permohonan menjadi cacat formal karena error in persona dan akibat lebih lanjut dari surat permohonan yang cacat formal adalah harus dinyatakan tidak dapat diterima.]
High-power and wide-temperature-range operations of InGaAsP-InP strained MQW lasers with reverse-mesa ridge-waveguide structure (1995)

A new structure for an InP-based ridge-waveguide laser is demonstrated. It has a reverse-trapezoid-ridge shape that offers reduced threshold current and smaller series resistance suitable for wide-temperature-range and high power operations. A 1.3 μm strained InGaAsP-InP MQW laser with the new ridge structure demonstrated over 200 mW output under a 440 mA injected current. Low threshold (<25 mA) and high efficiency (>0.3 W/A) operation was also achieved under 90/spl deg/C for 1.53 μm strained MQW lasers.
Haemolytic uraemic syndromes in the British Isles, 1985-8: association with verocytotoxin producing Escherichia coli. Part 2: Microbiological aspects. (1990)

In a three year study of children under 16 years with haemolytic uraemic syndrome faecal samples were examined for the presence of Verocytotoxin producing Escherichia coli (VTEC) using DNA probes and for free neutralisable Verocytotoxin in a Vero cell assay with specific antisera. There was evidence of VTEC infection in 58 of 185 (31%) samples. A total of 53 VTEC was identified from patients with haemolytic uraemic syndrome. Thirty eight VTEC belonged to serotype O157:H7 or O157:H-, 34 produced VT2 only, and four strains produced both VT1 and VT2. The remaining 15 VTEC belonged to nine different O serogroups; three strains produced VT1, 10 produced VT2, and two were positive for VT1 and VT2. Three control groups of patients without haemolytic uraemic syndrome were also examined. There was evidence of VTEC infection in 8%, 6%, and 4% of specimens from individuals with bloody diarrhoea, those with diarrhoea only, and healthy controls respectively. VTEC from the bloody diarrhoeal and diarrhoeal controls were O157:H7 but those from the healty controls could not be O serogrouped. This study confirms the association of VTEC, and particularly strains of O157:H7, with haemolytic uraemic syndrome. Strains producing VT1, VT2, or both toxins were isolated, although over 94% of VTEC produced VT2 alone or together with VT1.
Differential messenger RNA and protein expression of the receptor for advanced glycosylated end products in normal lung and non-small cell lung carcinoma. (1997)

The receptor for advanced glycosylated end products (RAGE), a member of the immunoglobulin superfamily, was one of the cDNA subtraction clones that was found to be differentially expressed in human lung and the corresponding tumor tissue. In nine additional matched normal/tumor pairs, a strongly reduced or missing expression, not only on a transcriptional level but also on a protein level, was demonstrated in the non-small cell lung carcinoma tissue. Because amphoterin, a physiological ligand of RAGE that is highly expressed in the lung, mediates cell differentiation via RAGE, a down-regulation of the receptor may be considered as a critical step in lung tumor formation. Furthermore, we determined the complete reading frame of RAGE.
Fluorescent thiophene-based materials and their outlook for emissive applications (2015)

Although thiophene-based materials are among the most widely studied conjugated materials for a number of technological applications, most discussions of emissive conjugated materials have focused on other systems, primarily due to the lower emission quantum yields of thiophene-based systems. Over the last decade, however, this has begun to change with the development of new highly emissive thiophene-based materials. In this review, we provide an overview of fluorescent thiophene-based materials and their applications, highlighting in particular the various methods employed to achieve highly emissive materials, as well as a variety of reported applications including fluorescent biomarkers and organic light emitting diodes.
Conclusions and recommendations (2009)

Government regulation is necessary to the economic life of all developed countries. However, although regulation is designed to benefit society at large, the costs of regulation, in particular the cost estimates employed in the policy-making process, are often hotly debated. Drawing on recent case studies, this innovative book examines the accuracy of regulatory cost estimates and explores in detail how these costs are calculated, the apparent relationship between ex post and ex ante calculations and why this might be.
Interaction of Sodium Dodecyl Sulfate with Poly(N-vinylpyrrolidone) and Poly(vinyl alcohol) in Aqueous Solutions (1972)

ポリピニルピロリドン(PVP)およびポリピニルアルコール(PVA)とドデシル硫酸ナトリウム(SDS)との水溶液中における相互作用を,高分子の固有粘度[η]および膜平衡法による結合量諾の測定によって研究した。その結果[η],xともにSDS濃度とともに増加し,極大値に達したのちふたたび低下することがわかった。また[η]は劣とともに増加し,この傾向はPVPにっいてとくに顕著であるが,PVAの場合には分子内水素結合のために[η]とxとの相関が悪いと結論した。等温結合曲線は遊離平衡濃度cfに対してに鐘状になるが,DS-アニオンの活動度α総.に対してはJ字型になった。これは濃厚なSDS溶液のDS-アニオンの活動度は低下するためである。複合体からのNa+イオンの電離度αpはm(o)とともに低下し,Na+イオンの活動度の対数log aNa+とlog(1-αp)/αpとは直線関係にあり,高分子弱電解質ようの性質を示すことがわかった。結合量xに対するDonnan効果の補正を試みたところ,SDSの臨界ミセル濃度以下ではDonnan効果を無視した場合にくらべて大きな差はなかった。
Warming enhances old organic carbon decomposition through altering functional microbial communities (2017)

Soil organic matter (SOM) stocks contain nearly three times as much carbon (C) as the atmosphere and changes in soil C stocks may have a major impact on future atmospheric carbon dioxide concentrations and climate. Over the past two decades, much research has been devoted to examining the influence of warming on SOM decomposition in topsoil. Most SOM, however, is old and stored in subsoil. The fate of subsoil SOM under future warming remains highly uncertain. Here, by combining a long-term field warming experiment and a meta-analysis study, we showed that warming significantly increased SOM decomposition in subsoil. We also showed that a decade of warming promoted decomposition of subsoil SOM with turnover times of decades to millennia in a tall grass prairie and this effect was largely associated with shifts in the functional gene structure of microbial communities. By coupling stable isotope probing with metagenomics, we found that microbial communities in warmed soils possessed a higher relative abundance of key functional genes involved in the degradation of organic materials with varying recalcitrance than those in control soils. These findings suggest warming may considerably alter the stability of the vast pool of old SOM in subsoil, contributing to the long-term positive feedback between the C cycle and climate.
Long-Base Laser Strainmeters: A Review (2003)

This document reviews the history, design, and use of the long-base laser strainmeters developed at IGPP/SIO since 1970: the longest-running and most stable examples of such instruments. We describe the principles of operation, limitations on dynamic range the frequency response, and major sources of error, along with the design and construction methods used. We also provide results on reliability of operation and the methods used to produce final high-quality data from what is recorded in the field: both based on close to 100 instrument-years of experience. We describe the setting of the two long-running instruments in California, at Pin~on Flat Observatory and at Durmid Hill; for both locations we summarize results on long-term strain, tidal strains, and various strain ``events'': the last almost all related to earthquakes, since the results of these high-quality measurements have shown few departures from steady strain accumulation with time.
A Novel Saliva Test for Caries Risk Assessment (2006)

A new saliva test for caries risk assessment introduced in this study integrates a variety of host factors to predict for children, individual risk levels that are tooth-group specific. These various host factors correlate with caries history, DFT (decayed and filled teeth) or DFS (decayed and filled surfaces) in young adults. The test is based on the pattern of genetically determined oligosaccharides present on salivary glycoproteins. The mechanism behind the test is believed to be centered on the specific oligosaccharides that either facilitate bacterial attachment and colonization at the surface of teeth or protect against colonization by promoting agglutination and removal of free bacteria. It is the ratio of the two classes of oligosaccharides that is very strongly correlated with the numerical range of DFS or DFT observed in a young adult population.
Multi-Core Fiber Rotated Optical Switch for Remote Optical Fiber Switching (2021)

We propose an optical switch that rotates an MCF set in a cylindrical ferrule. We show the loss characteristics and examine the factor of the excess loss focusing on an offset between the each core.