gebhart/patrepeval huggingface dataset:
Re-Investigation of the Protein Structure of Coenzyme B12-Dependent Diol Dehydrase (1987)

We have purified diol dehydrase, an adenosylcobalamin-dependent enzyme, from Klebsiella pneumoniae by two different procedures to re-investigate its protein structure; one including its extraction with detergent from the membrane fraction, and the other consisting of only chromatographic separations of the soluble fraction. The enzyme preparations obtained by these two methods were different in the subunit structure, but both are identical in molecular weight, and in-enzymological and immunochemical properties. In addition, the enzyme preparation obtained from the membrane fraction dissociated reversibly into two dissimilar protein components (F and S) in the absence of substrate, as did the preparation from the soluble fraction. Although the subunit multiplicity of component S might be partly due to proteolytic cleavage during the enzyme purification as revealed by limited digestion with trypsin, component F is not a product of proteolytic cleavage of component S, but a primordial and essential constituent of the enzyme.
Shortest Path Algorithms: An Evaluation Using Real Road Networks (1998)

The classic problem of finding the shortest path over a network has been the target of many research efforts over the years. These research efforts have resulted in a number of different algorithms and a considerable amount of empirical findings with respect to performance. Unfortunately, prior research does not provide a clear direction for choosing an algorithm when one faces the problem of computing shortest paths on real road networks. Most of the computational testing on shortest path algorithms has been based on randomly generated networks, which may not have the characteristics of real road networks. In this paper, we provide an objective evaluation of 15 shortest path algorithms using a variety of real road networks. Based on the evaluation, a set of recommended algorithms for computing shortest paths on real road networks is identified. This evaluation should be particularly useful to researchers and practitioners in operations research, management science, transportation, and Geographic Information Systems.
Risk Management at Railroad Grade Crossings: Proposal for a Decision Support System (2016)

Managing accident risk at Railway Level Crossings (RLCs) has been an important issue for both railway and highway infrastructure managers and operators. The term risk management of RLCs includes the process which is followed, the means used and measures taken in order to achieve a specific goal related to the aspect of safety of RLCs. Improvement of the safety level can be reached either by reducing the probability of having an accident or by reducing the consequences of the accidents or by their combination. Safety improvement is costly, however what is not often known is its correlation with the required cost. This paper presents a scientific "tool" for managing the safety of RLCs. A methodology is developed in order to assist the railway infrastructure managers to decide on the type and size of the interventions to be made at a level crossing of the railway network in order to improve its safety. The methodology uses the conventional evaluation indicators of the economic efficiency of an investment/project (NPV; IRR; cost/benefit ratio) as a criterion for the decision making; at the same time it enables the correlation of these indicators to the improvement of the safety level. The methodology is then applied to RLCs with specific constructional and operational characteristics. The proposed methodology may be applied in all incident categories and for various causes subject to appropriate modifications.
SRIF-like immunoreactivity in the brain and pituitary of Tinca tinca (Cyprinidae, Teleostei). (1990)

SRIF-immunoreactive perikarya occurred in the nucleus entopeduncularis, the nucleus praeopticus and praeopticus periventricularis, in the nucleus lateralis tuberis pars anterior, the nucleus ventromedialis, and in the nucleus posterior periventricularis. Additionally, SRIF-containing neurons were located in the nucleus dorsomedialis and dorsolateralis thalami. Weaklyimmunoreactive perikarya were found medially to the nucleus diffusus tori lateralis and in the rostral rhombencephalon, laterally to the fasciculus longitudinalis medialis, and dorsally to the fasciculus longitudinalis lateralis. SRIF-positive fibres could be found in the lateral and dorsal telencephalon, below most of the diencephalic nuclei, in the pituitary, the tectum opticum, the torus semicircularis, and in the lateral medulla oblongata.
Registration of ‘CL271’ Rice (2015)

‘CL271’ (Reg. No. CV-147, PI 672550), a Clearfield (BASF, Ludwigshafen, Germany) medium-grain rice (Oryza sativa L.) cultivar, was developed by the Louisiana State University Agricultural Center at the Rice Research Station near Crowley, LA, and approved for release by the director of the Louisiana Agricultural Experiment Station in 2011. CL271 was derived from the cross ‘Neptune’//’Bengal’//’CL161’ made in 2009 and advanced in 2011 as an F2:3 bulked selection. Testing in the Clearfield preliminary yield trial in 2011 indicated high yield potential and favorable agronomic characteristics. In 2012 and 2013, CL271 was evaluated in 11 statewide yield performance trials, along with check cultivars ‘CL261’, ‘Caffey’, and ‘Jupiter’. Grain yield of CL271 was 10.2 Mg ha−1 compared with 9.0, 8.4, and 9.2 Mg ha−1 for CL261, Caffey, and Jupiter, respectively. Plant height for CL271 was 97 cm compared with 99 cm for CL261, Caffey, and Jupiter. CL271 reached 50% heading in 90 d, whereas CL261 reached 50% heading in 87 d, and Caffey and Jupiter in 92 d. CL271 has a semi-erect flag leaf, and the leaves are dark green and display an intermediate leaf angle. The lemma and palea are straw-colored and glabrous, and the apiculus, light purple at heading, is colorless as the grain approaches harvest maturity. CL271 is resistant to blast disease (caused by Magnaporthe grisea), moderately susceptible to sheath blight (caused by Rhizoctonia solani), and susceptible to bacterial panicle blight (caused by Burkholderia spp.).
Unexpected catalytic site variation in phosphoprotein phosphatase homologues of cofactor‐dependent phosphoglycerate mutase (2003)

The cofactor-dependent phosphoglycerate mutase (dPGM) superfamily contains, besides mutases, a variety of phosphatases, both broadly and narrowly substrate-specific. Distant dPGM homologues, conspicuously abundant in microbial genomes, represent a challenge for functional annotation based on sequence comparison alone. Here we carry out sequence analysis and molecular modelling of two families of bacterial dPGM homologues, one the SixA phosphoprotein phosphatases, the other containing various proteins of no known molecular function. The models show how SixA proteins have adapted to phosphoprotein substrate and suggest that the second family may also encode phosphoprotein phosphatases. Unexpected variation in catalytic and substrate-binding residues is observed in the models.
Presentation mining tool: from slides to mind maps (2016)

Slide presentations such as the PowerPoint presents knowledge by the subject matter expert in a linear sequence. Nonetheless, post-presentation, the knowledge is actually reconstructed differently by the learners depending on their personal understanding over the entire presentation. To enhance learning, this paper presents a presentation mining tool that is able to extract keywords and key phrases from a collection of Microsoft PowerPoint slides and generate a mind map with topics and subtopics based on the extracted keywords. The mind map produced by the system contains the main topic as the central node of the map with branches connecting main topic to subtopics. The mind map produced through automatic extraction of topic and subtopics is aimed to help learners to concentrate on the entire concept rather than following through the slides sequentially.
Magnitude of Dopamine Release in Medial Prefrontal Cortex Predicts Accuracy of Memory on a Delayed Response Task (2004)

Modulation of neural function in the prefrontal cortex (PFC) by dopamine (DA) is essential for higher cognitive processes related to attention, working memory, and planning of future behavior. The present study demonstrates that DA efflux in the PFC is increased in a phasic manner when a rat engages in search behavior for food reward on an eight arm radial maze guided by memory, independent of whether or not reward is obtained for making the correct choice. Furthermore, disruption of accurate recall of the correct pattern of arms induced by increasing the delay period from 30 min to 1 or 6 hr, is associated with attenuated DA efflux during the retrieval phase of the task. The observed increase in DA efflux in the absence of reward at a 30 min delay and the minimal increase during consumption of the same quantity of food during poor performance after an unexpected 6 hr delay, argue against a simple relationship between DA function in the PFC and reward processes. Instead, these data demonstrate a close functional relationship between the release of DA from terminals within the PFC and the retrieval of specific trial unique memories; furthermore, the magnitude of mesocortical DA efflux is predictive of the accuracy of this form of memory.
Up-Conversion Visible et Infra-Rouge Dans Des Fibres en Silice Codopees Tm3+: Yb3+ Sous Une Excititation a 980 mm (2008)

Alcohol-induced increases in nucleus accumbens glutamate actively regulate alcohol consumption, and the alcohol responsiveness of corticoaccumbens glutamate systems relates to genetic variance in alcohol reward. Here, we extend earlier data for inbred mouse strain differences in accumbens glutamate by examining for differences in basal and alcohol-induced changes in the striatal expression of glutamate-related signaling molecules between inbred C57BL/6J and DBA2/J mice. Repeated alcohol treatment (8 × 2 g/kg) increased the expression of Group1 metabotropic glutamate receptors, the NR2a/b subunits of the N-methyl-D-aspartate receptor, Homer2a/b, as well as the activated forms of protein kinase C (PKC) epsilon and phosphoinositol-3-kinase within ventral, but not dorsal, striatum. Regardless of prior alcohol experience, C57BL/6J mice exhibited higher accumbens levels of mGluR1/5, Homer2a/b, NR2a and activated kinases vs. DBA2/J mice, whereas an alcohol-induced rise in dorsal striatum mGluR1/5 expression was observed only in C57BL/6J mice. We next employed virus-mediated gene transfer approaches to ascertain the functional relevance of the observed strain difference in accumbens Homer2 expression for B6/D2 differences in alcohol-induced glutamate sensitization, as well as alcohol preference/intake. Manipulating nucleus accumbens shell Homer2b expression actively regulated these measures in C57BL/6J mice, whereas DBA2/J mice were relatively insensitive to the neurochemical and behavioral effects of virus-mediated changes in Homer2 expression. These data support the over-arching hypothesis that augmented accumbens Homer2-mediated glutamate signaling may be an endophenotype related to genetic variance in alcohol consumption. If relevant to humans, such data pose polymorphisms affecting glutamate receptor/Homer2 signaling in the etiology of alcoholism.
Strategy Integrating Stepped Fragmentation and Glycan Diagnostic Ion-Based Spectrum Refinement for the Identification of Core Fucosylated Glycoproteome Using Mass Spectrometry (2014)

Core fucosylation (CF) is a special glycosylation pattern of proteins that has a strong relationship with cancer. The Food and Drug Administration (FDA) has approved the core fucosylated α-fetoprotein as a biomarker for the early diagnosis of hepatocellular carcinoma (HCC). The technology for identifying core fucosylated proteins has significant practical value. The major method for core fucosylated glycoprotein/glycopeptide analysis is neutral loss-based MS(3) scanning under collision-induced dissociation (CID) by ion trap mass spectrometry. However, due to the limited speed and low resolution of the MS(3) scan mode, it is difficult to achieve high-throughput, with only dozens of core fucosylated proteins identified in a single run. In this work, we developed a novel strategy for the identification of CF glycopeptides at a large scale, integrating the stepped fragmentation function, one novel feature of quadrupole-orbitrap mass spectrometry, with "glycan diagnostic ion"-based spectrum optimization. By using stepped fragmentation, we were able to obtain both highly accurate glycan and peptide information of a simplified CF glycopeptide in one spectrum. Moreover, the spectrum could be recorded with the same high speed as the conventional MS(2) scan. By using the "glycan diagnostic ion"-based spectrum refinement method, the efficiency of the CF glycopeptide discovery was significantly improved. We demonstrated the feasibility and reproducibility of our method by analyzing CF glycoproteomes of mouse liver tissue and HeLa cell samples spiked with standard CF glycoprotein. In total, 1364 and 856 CF glycopeptides belonging to 702 and 449 CF glycoproteins were identified, respectively, within a 78-min gradient analysis, which was approximately a 7-fold increase in the identification efficiency of CF glycopeptides compared to the currently used method. In this work, we took core fucosylated glycopeptides as a practical example to demonstrate the great potential of our novel method for use in glycoproteome analysis, and we also anticipate using the flexible novel method in other research fields.
Venous Thromboembolism in Patients with Acute Thoracolumbar Spinal Cord Injury. (2018)

Venous thromboembolism (VTE) is a major complication in patients with acute spinal cord injury. There are few reports of VTE with acute thoracolumbar spinal cord injury (TLSCI). We assessed the incidence of VTE with acute TLSCI using color Doppler ultrasonography. We retrospectively assessed 75 patients with acute TLSCI (T1 to L1). All patients were surgically treated. VTE of the lower extremity and pelvis was assessed using color Doppler ultrasound regardless of whether symptoms were present. This retrospective study included patients who were assessed between 6 and 10 days (mean 8.1 days) after injury. VTE was detected in 27 of the 75 patients (35.7%) with or without paralysis. Of the 13 patients who had complete motor paralysis, 8 (62%) had VTE; of the 31 patients with incomplete motor paralysis, 10 (32%) had VTE, and of the 31 patients without motor paralysis, 9 (29%) had VTE. Among the patients with TLSCI, those with VTE had a significantly higher mean age than those without. The incidence of VTE in TLSCI patients is not related to the severity of paralysis in a Japanese population. The incidence appears to be related primarily to age.
Estimated Organ Donor Potential in German Maximum Care Hospitals (2012)

Background: The lack of suitable organs remains one of the major problems transplantation medicine. One step to solve this problem is the increase in the identification of potential organ donors. An previously published American analysis has shown that clinics with more than 350 beds have an average potential of 0.015 organ donors/bed. Adopting this model to our german conditions, the potential of organ donors for maximum care hospitals was estimated. Methods: The bed capacity of university hospitals (A-hospitals) and hospitals providing a neurosurgical department (B-hospitals) was determined by internet search. The number of beds was multiplied by factor 0.015 (if bed capacity was >350 beds) or factor 0.012 (if bed capacity was 150-349 beds). Based on this data, the organ donor potential for every clinic was calculated. Data about the number of organ donors were ascertained by the annual reports of the Deutsche Stiftung Organtransplantation and compared to the calculated organ donor potential in 2010. Results: At all 397 organ donations were realized in 35 university clinics whereas 107 B-hospitals accounted for 555 organ donors in 2010. Total bed capacity for university clinics was 46240 beds according to 0.008 organ donors/bed while B-clinics with a total of 77720 beds provided 0.07 donors/bed. Comparing calculated organ donor potential to actual organ donors in 2010, university clinics have a potential of 694 organ donors whereas only 57% of the calculated potential is utilized. B-clinics have only reached 48% of their calculated potential of 1156 organ donors. Considering the range of variation between individual clinics a potential use of 9-128% with 1-20 organ donors can be pointed out for university hospitals. B-clinics showed even a greater variety of potential use with 7-250% identifying 1-18 organ donors in 2010. Conclusions: These results indicate that there is still a potential to increase the rate of organ donors in Germany with regard to university hospitals and B-Hospitals. Single clinics seem to face more difficulties with the process of identification of organ donors than others. Actual changes in organizational structure with implementation of inhouse-coordinators are expected to achieve further improvement in number of organ donations.
Buckling Stability of Adhesively Bonded Composite Conical Shells Under Both Axial Compressive and External Pressure Loads (2006)

A mathematical model is developed for buckling stability of an adhesively bonded composite conical shell system consisting of carbon fiber in a polyimide (PI) matrix. The conical shell is modeled by two orthotropic layers bonded together with a PI adhesive. In-plane compressive and external surface pressure loads are applied. The adhesive layer is modeled by mechanical linear normal and shear springs. The Galerkin method is applied to analyze the buckling stability of the structure as an eigenvalue problem to give critical buckling loads and corresponding mode shapes. Parametric studies are performed to show effects of system parameters on buckling stability of the system.
Multilingual BERT Post-Pretraining Alignment (2021)

Lin Pan, Chung-Wei Hang, Haode Qi, Abhishek Shah, Saloni Potdar, Mo Yu. Proceedings of the 2021 Conference of the North American Chapter of the Association for Computational Linguistics: Human Language Technologies. 2021.
Catalytic Supramolecular Gels (2021)

Supramolecular gels, in particular those formed by low-molecular compounds, offer unique opportunities in the field of catalysis. Catalytic low-molecular weight gels (LMWGs) are materials formed by the self-assembly of synthetically simple compounds bearing catalytic or pre-catalytic fragments into functional supramolecular materials. Those materials may find application in organocatalysis as well as metallocatalysis, either as a single component or, combined with other supramolecular entities, as a multifunctional material.
Preparation and chromatographic properties of a multimodal chiral stationary phase based on phenyl‐carbamate‐propyl‐β‐CD for HPLC (2007)

A phenylcarbamate derivative of 2-hydroxypropyl-beta-CD bonded stationary phase was prepared by a previously described method. Its enantiomeric recognition abilities were evaluated as chiral stationary phase (CSP) in normal, polar organic and RP conditions by HPLC. The relevant structural features of the prepared stationary phase which make it an effective chiral selector are discussed. This material seems to have an excellent enantioselectivity for a variety of racemic analytes in the three modes. Hence it can be considered a highly effective multimodal column. Retention factor (k), selectivity (alpha) and resolution (R(s)) were the chosen parameters to describe the column performance. Optimization of these separations was discussed in terms of mobile phase composition, flow rate and structural patterns of the injected analytes.
Glycosylation-mediated phenylpropanoid partitioning in Populus tremuloides cell cultures (2009)

Exogenous salicyl alcohol was readily glycosylated in Populus cell cultures, a process that altered sugar utilization and phenolic partitioning in the cells. Using this system, we identified candidate genes for glycosyltransferases that may mediate the glycosylation, and for transporters that mediate the subcellular compartmentalization of sugars and phenolic glycosides. The suspension cells appear to represent a facile system for dissecting the regulation of phenolic carbon partitioning, and in turn, its effects on growth in Populus.
Magnetic resonance imaging of transfusional hepatic iron overload (1994)

The transfusional iron overload in 11 adult patients suffering from haematological malignancy was studied in 14 consecutive 1.0 Tesla magnetic resonance imaging (MRI) studies. Routine T1- and T2-weighted spin echo sequences and a fat suppressed sequence (STIR) were used. The ratio from a region of interest of the liver parenchyma and from a manganese chloride reference phantom was measured. There was a definite difference between the signal ratio from the haematological patient group and the control group (p = 0.0001). The patients showed a diminished signal ratio in all pulse sequences used. There was a statistically significant inverse linear correlation between the number of transfused red cell units and the signal ratio in the T1-weighted (p = 0.03) and the T2-weighted (p = 0.03) images. A definite decrease of the effect is found after 20-30 red cell unit transfusions in T2-weighted and STIR images.
Electronic spectra of 2-aminonaphthalene-6-sulfonate and related molecules. I. General properties and excited-state reactions (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic spectra of 2-aminonaphthalene-6-sulfonate and related molecules. I. General properties and excited-state reactionsCarl J. Seliskar and Ludwig BrandCite this: J. Am. Chem. Soc. 1971, 93, 21, 5405–5414Publication Date (Print):October 1, 1971Publication History Published online1 May 2002Published inissue 1 October 1971https://pubs.acs.org/doi/10.1021/ja00750a016https://doi.org/10.1021/ja00750a016research-articleACS PublicationsRequest reuse permissionsArticle Views276Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
New complex-coupled DFB-laser with a contacted surface grating for lambda =1.55 µm (1995)

A novel complex-coupled InGaAsP/InP integrated twin guide distributed feedback (ITG-DFB) laser with a substantially simplified fabrication process made by single step epitaxy without corrugation overgrowth is described. Room temperature CW operation with a threshold current of 45 mA and a SMSR in excess of 40 dB is demonstrated.
Analysis of Low-Density Parity-Check Codes Based on EXIT Functions (2006)

We exploit extrinsic information tranfer functions of single parity-check and prepetition codes over the binay input additive white Gaussian noise (biAWGN) channel, for asymptotic performance analysis of belief propagation decoding of low-density parity-check codes. The approach is based on a Gaussian approximation (GA) of the density evolution algorithm using the mutual information measure. We show that our method allows more accurate prediction of the decoding threshold in the biAWGN channel than the earlier known GA methods.