gebhart/patrepeval huggingface dataset:
Differential Gene Expression in Microglial Polarized States and in Alzheimer’s Disease: Novel Insights Provided by Weighted Correlation Network Analysis (WGCNA) (P4.011) (2016)

OBJECTIVE To use systems biology to identify molecular profiles of pro-inflammatory (M1) and phagocytic/restorative (M2) microglia in AD and explore differential ion channel expression that may contribute to their regulation. BACKGROUND Microglia have opposing actions in AD: pro-inflammatory M1 state invoked in neurodegeneration, and phagocytic M2 state that mediates Aß-clearance and neuroprotection. To clarify molecular profiles of these extreme microglial phenotypes in AD, we used network analysis to identify modules of co-expressed genes that provide a molecular signature of M1/M2 microglia, and to identify these cell-specific modules in AD mouse brain. We also explored cationic channel (potassium/purinergic/TRP) expression in these modules since they regulate microglial functions (ROS production and chemotaxis). METHODS Two published primary microglial microarray datasets were utilized. In study 1, microglia were exposed to LPS (M1-polarizing), IL-4 (M2-polarizing) or Control for 48h (n=3/group). Study 2 used microglia from wild-type, AD (5xFAD), TREM2-/- and 5xFAD/Trem2-/- mice (n=5/group). Gene microarray data from each condition were normalized using the RMA function in the affy()-R library followed by removal of batch-specific systematic differences using ComBat algorithm. WGCNA was performed with optimized parameters across arrays. RESULTS Twenty modules of 21700 co-expressed genes were identified including one AD-specific pro-inflammatory module (containing AD-related [CD14, COX-2, IRG1], K-channel Kv1.3 genes), one AD-specific anti-inflammatory module (containing TYROBP, IGF-1, CD200R, K-channel Kir2.1 and purinergic channel P2RX4); and two LPS-specific modules containing M1 marker ICAM-1, inflammasome (NLRP3, Caspase-11, GBP4/5) and apoptotic (MyD88) genes. Network analysis placed AD-specific pro-inflammatory module genes downstream of β1-integrin activation, a process coupled to Kv1.3 function. Selective Kv1.3 blockade inhibited Aβ42-induced microglial ROS production and rescued microglia from Aβ42-induced gap closure-inhibition. CONCLUSIONS Our results define signature molecular networks of M1 microglial states in addition to unique AD-specific pro- and anti-inflammatory profiles, with putative ion channel targets predicted to regulate AD-specific modules (pro-inflammatory: Kv1.3, anti-inflammatory: Kir2.1/P2RX4).
Comparison of the Efficacy of Different Therapeutic Regimen in the Management of Glaucoma in Dogs (2020)

Eighteen dogs presented to the Veterinary College Hospital, Bangalore exhibiting clinical signs of glaucoma and IOP more than 25 mmHg were selected. These dogs were divided into 3 groups of 6 animals each and were subjected to therapeutic management of glaucoma. Group I was treated with Timolol Maleate ophthalmic solution, 1 drop, BID, Group II was treated with Dorzolamide and Timolol, 1 drop, BID and Group III was treated with Latanoprost and Timolol, 1 drop, BID . The duration of therapy was for 2 months and the dogs were monitored every once in 15 days i.e., day 0, day 15, day 30, day 45 and day 60 following initiation of therapy. It was found that Group III dogs treated with Latanoprost and Timolol combination responded better than the other two groups followed closely by Group II dogs treated with Dorzolamide and Timolol combination whereas monotherapy with Timolol was not very effective.
Impact of Reconstruction With Hydroxyapatite Bone Cement on CSF Leak Rate in Retrosigmoid Approach to Vestibular Schwannoma Resection: A Review of 196 Cases (2021)

CSF leak rate following acoustic neuroma resection was significantly reduced by introduction of Cranios hydroxyapatite bone cement.
The Care Ecosystem: Promoting self-efficacy among dementia family caregivers (2018)

Self-efficacy represents a family caregiver's knowledge and preparedness in managing the challenges of care. Psychosocial support shows benefit in improving caregiver self-efficacy that in turn, may positively influence caregiver health and well-being. The findings in this manuscript demonstrate how a model of navigated care can positively impact self-efficacy among dementia family caregivers.
SUBUNIT FUNCTION AND STRUCTURE OF EUCARYOTIC RNA POLYMERASE IIA (1981)

Conference Abstract| April 01 1981 SUBUNIT FUNCTION AND STRUCTURE OF EUCARYOTIC RNA POLYMERASE IIA Erik A. Bateman; Erik A. Bateman 1Dept. of Phys. & Biochem. Univ. of Reading, Whiteknights, Reading, U.K. Search for other works by this author on: This Site PubMed Google Scholar Bruce H. Nicholson Bruce H. Nicholson 1Dept. of Phys. & Biochem. Univ. of Reading, Whiteknights, Reading, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1981) 9 (2): 171P. https://doi.org/10.1042/bst009171p Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation Erik A. Bateman, Bruce H. Nicholson; SUBUNIT FUNCTION AND STRUCTURE OF EUCARYOTIC RNA POLYMERASE IIA. Biochem Soc Trans 1 April 1981; 9 (2): 171P. doi: https://doi.org/10.1042/bst009171p Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1981 Biochemical Society1981 Article PDF first page preview Close Modal You do not currently have access to this content.
Hydrothermal synthesis of zinc oxide-reduced graphene oxide nanocomposites for an electrochemical hydrazine sensor (2015)

A facile hydrothermal method is utilized to synthesize zinc oxide-reduced graphene oxide nanocomposites for an electrochemical hydrazine sensor.
Deep Q Learning Based High Level Driving Policy Determination (2018)

With the commercialization of various Driver Assistance Systems (DAS), those vehicles have some autonomous functions like Smart Cruise Control (SCC) and Lane Keeping System (LKS). It is believed that autonomous driving can be achieved by combining the DAS functions in the limited situations such as on highways. However, in order to coordinate the DAS functions for autonomous driving, a supervisor is needed to select an appropriate DAS function. In this paper, we propose a method for training a supervisor that selects proper DAS by deep reinforcement learning. The driving policy operates based on camera images and LIDAR data that are accessible in autonomous vehicles. Therefore, deep reinforcement learning network model is designed to analyze both camera image and LIDAR data. This system aims to drive in simulated traffic situation of highway without collision and with high speed. Unlike the systems which learn how to throttle, brake and steering directly, the proposed method can guarantee safe driving because the learned driving policy is based on the existing commercialized DAS functions. In order to verify the algorithms, a simulation tool is developed using Unity for highway environment with multiple vehicles and autonomous driving performance is compared with the proposed supervisor.
D‐mannose consumption and bacterial anti‐adhesion activity in human urine (2008)

Ingestion of D‐mannose has been hypothesized to prevent bacterial adhesion in the urinary tract, since it is the uroepithelial cell receptor for Type 1 E. coli . There are few, if any, studies on effects of D‐mannose on UTI prevention, and it is questionable whether sufficient urinary concentrations are achieved to elicit an anti‐adhesion effect. The aim of this study was to determine if consumption of D‐mannose alters urinary bacterial adhesion activity. D‐mannose was administered (1,000 mg, 2,000 mg, and 4,000 mg) in single doses on subsequent days, with 3‐d wash‐out periods between doses. Urines were collected prior to mannose consumption and every 3 h for 12 h, and again at 24 h following ingestion. Urines were tested utilizing a mannose‐sensitive guinea pig red blood cell hemagglutination assay specific for Type 1‐fimbriated E. coli . Effective anti‐adhesion concentrations of D‐mannose were determined by a dilution series, followed by an activity threshold. Urinary pH averaged 6.5, eliminating a bacteriostatic effect. Over 50% of urines demonstrated anti‐adhesion activity; but, there was no relationship between level of activity and amount of mannose ingested. This suggests the measured anti‐adhesion activity was a “background” effect caused by endogenous excretion of Tamms‐Horsfall glycoprotein, and that ingested mannose did not affect anti‐adhesion activity. This work was supported by Theralogix, LLC.
Interleavers for turbo codes using permutation polynomials over integer rings (2005)

A class of deterministic interleavers for turbo codes (TCs) based on permutation polynomials over /spl Zopf//sub N/ is introduced. The main characteristic of this class of interleavers is that they can be algebraically designed to fit a given component code. Moreover, since the interleaver can be generated by a few simple computations, storage of the interleaver tables can be avoided. By using the permutation polynomial-based interleavers, the design of the interleavers reduces to the selection of the coefficients of the polynomials. It is observed that the performance of the TCs using these permutation polynomial-based interleavers is usually dominated by a subset of input weight 2m error events. The minimum distance and its multiplicity (or the first few spectrum lines) of this subset are used as design criterion to select good permutation polynomials. A simple method to enumerate these error events for small m is presented. Searches for good interleavers are performed. The decoding performance of these interleavers is close to S-random interleavers for long frame sizes. For short frame sizes, the new interleavers outperform S-random interleavers.
Synthesis of Novel Steroid Alkaloids by Cyclization of Arylimines from Estrone (1999)

Intramolecular Lewis or Brønsted acid-catalyzed cyclization reactions of steroid arylimines 6 yielded either tetrahydroquinolines condensed to the estrane skeleton 9 or N-arylamino-D-homosteroids 12–16, depending on the substituent of the arylimino group.
Optimization of Strawberry Volatile Sampling by Direct Gas Chromatography Olfactometry (2008)

International audience
Growth of carbon allotropes and plasma characterization in linear antenna microwave plasma CVD system (2014)

Growth of diamond coatings with tunable morphology and concurrent substrate catalyst pretreatment and further growth of carbon nanotubes were studied. The dependence of plasma parameters on gas composition was studied by Langmuir probe measurement. Grown diamond coatings and carbon nanotubes were characterized by scanning electron microscopy and Raman spectroscopy and correlated with process parameters. Well-defined nanocrystalline, polycrystalline, and porous diamond films were prepared. Concurrent substrate catalyst pretreatment and further growth of carbon nanotubes were shown.
The influence of bioglass on the growth of fibroblasts (1987)

The influence of Bioglass (BG) on the growth of L cells was studied by means of tissue culture, to get an idea on its bone-bonding mechanism. Growth rate of L cells on BG was lower than that on the control silica glass (SG). L cells on BG diminished in size and took up long and slender polygonal shapes with radially spread long pseudopods, which probably meant that the cell cycle was detained at the synthetic period. From these findings, BG is thought to have an inhibitory effect on the growth of L cells, which might arrest the fibrous tissue encapsulation and allow bonding with bone.
Using Digital Measurement–Based Care for the Treatment of Anxiety and Depression in Children and Adolescents: Observational Retrospective Analysis of Bend Health Data (2023)

As increasing numbers of young people and families seek DMHIs over traditional mental health treatments due to their accessibility and affordability, this study offers promising early evidence that youth anxiety symptoms decrease during involvement in an MBC DMHI such as Bend Health Inc. However, further analyses with enhanced longitudinal symptom measures are necessary to determine whether depressive symptoms show similar improvements among those involved in Bend Health Inc.
Abstract P4-13-20: Activity of HP-based therapies as third and later lines for the treatment of HER2-positive metastatic breast cancer: A retrospective study from a single institution (2016)

Abstract Background: Dual anti-HER2 blockade with trastuzumab and pertuzumab (HP) plus chemotherapy is an effective therapy (Rx) in the 1st-line setting for HER2-positive metastatic breast cancer (MBC). Our single arm phase II study included patients (pts) treated with HP plus paclitaxel in the 2nd-line setting with progression-free survival (PFS) benefit. Recently, we reported results from a retrospective study of pts treated at our institution, suggesting a longer PFS for those who received HP-based Rxs when compared to any other anti-HER2 based Rxs in the 2nd-line setting. To further assess the activity of this combination in later Rx lines, we conducted a retrospective analysis of pts with HER2-positive MBC who had progressive disease after 2nd-line and were treated with HP-based Rxs in the 3rd and later lines at MSKCC. Historically, the median (med) PFS in this setting with trastuzumab-based Rx is about 3-4 months. Methods: Pts diagnosed with HER2-positive MBC and treated with HP-based Rxs at MSKCC between 1-1-2011 and 03-30-2015 and who progressed on 2nd-line Rx were identified through an institutional database. Primary endpoint was PFS in 3rd and later treatment lines. Results: 70 pts who received any HP-based Rx in the 3rd or later lines of treatment were eligible. The med number of prior anti-HER2 Rx was 3. The baseline characteristics and Rxs are summarized in Table 1. The med PFS for the entire cohort was 5.7 months (95% CI, 4.8-6.5). Conclusions: In this retrospective analysis involving heavly pretreated patients, HP-based Rx appears to be an active regimen and compares favorably to historical data. This supports the NCCN endorsement of HP-based Rx in later lines if HP has not been delivered previously. Baseline Characteristics and treatments (n=70)Characteristic(n)(%)Age Median56 Range27-83 Gender Female70100.0Male00.0Race Black1014.3White5477.1Other68.6Ethnicity Non Hispanic6694.3Hispanic45.7ER/PR status ER and PR negative2738.6ER and/or PR positive4361.4Type of disease Non visceral1622.9Visceral5477.1Visceral CNS2130.0Anti-HER2 in early stage Yes2941.4No4158.6Line of therapy 3rd line1927.14th line1521.45th line912.96th line912.97th line811.48th line and beyond1014.3HP-based regimens Chemotherapy + HP5477.1Endocrine therapy + HP57.1HP alone912.9Other22.9 Citation Format: Argolo D, Friedman M, Smyth L, Iyengar N, Singh J, Patil S, Norton L, Baselga J, Hudis C, Dang C. Activity of HP-based therapies as third and later lines for the treatment of HER2-positive metastatic breast cancer: A retrospective study from a single institution. [abstract]. In: Proceedings of the Thirty-Eighth Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2015 Dec 8-12; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2016;76(4 Suppl):Abstract nr P4-13-20.
Impact of Drug Formulation Variables on Silicone Oil Structure and Functionality of Prefilled Syringe System (2017)

Use of prefilled syringes to self-administer biologics via subcutaneous administration provides convenience to patients. The barrel interior of prefilled syringes is typically coated with silicone oil for lubrication to aid plunger movement at the time of administration. This study intended to evaluate the impact of formulation variables on the silicone oil on the barrel interior surface. Characterization techniques including syringe glide force, break loose force, Schlieren imaging, contact angle, inductively coupled plasma spectrometry, and thin film interference reflectometry were used in assessing the interactions. Data indicated that formulation variables such as pH, buffer/tonicity agent type and concentration, and surfactant present in the formulation can effect silicone oil lubrication of prefilled syringes, leading to changes in functional properties of the syringe over time. Syringe samples containing acetate and histidine buffers showed an increase in glide force at accelerated storage temperature conditions, but the change was minimal at 5 °C. The samples with the highest glide force correlated with the presence of mannitol in combination with sodium acetate buffer. Sodium chloride had lesser impact on glide force than mannitol. Samples with higher glide force exhibited a substantial change in the silicone oil layer of the syringe, as observed with Schlieren imaging, as well as a significant reduction in surface hydrophobicity, as demonstrated through contact angle measurement. These data indicated that the structure of the siliconized surface can change over time in contact with different formulations. During formulation development of drug products in prefilled syringes, in addition to potential impact on molecule stability, the selection of formulation variables should also be guided by assessing the impact to syringe functionality with the glide force as one of the key parameters.<i>LAY ABSTRACT:</i> Self-administering drug products packaged in prefilled syringes provides convenience to patients. The interior of a prefilled glass syringe is typically lubricated with silicone oil for easy plunger movement during injection. This article discusses the impact of formulation excipients on silicone oil coating inside the syringe. Characterization techniques were used to assess the ease of plunger movement and structure of the silicone coating. Data indicate formulation excipients can affect silicone oil distribution of prefilled syringes, leading to an increase in plunger glide force at accelerated storage temperature conditions. The increase in glide force within a prefilled syringe with or without an auto-injector can have an impact on dose accuracy and user experience. Syringes with a higher plunger glide force appeared to exhibit a change over time in surface energy and structure of the silicone oil layer in contact with particular formulations.
Impact of Second Harmonic Imaging on the Determination of the Global and Regional Left Ventricular Function by 2D Echocardiography: a Comparison with MIBI Gated SPECT (2000)

The use of SH echocardiography decreases the number of unscored segments. This results in an important gain in correlation and agreement for EF determination between echo and SPECT, and in a considerable decline of the interobserver variability for the echo-determined WMSI. WMSI determined by MIBI gated SPECT correlated closely with the SH WMSI, and agreement between both methods was excellent.
Microfluidic devices for biomolecular and cellular analysis (2016)

In this thesis microfluidic chips were developed for tissue sampling, chemical separation and electrical cell stimulation. Several fluorescence measurement systems were developed in order to enable small volume measurements in microchannels. &#13;\n&#13;\nMicroneedle sampling was developed for cancer tissue analysis. A novel two channel silicon microneedle structure was designed and fabricated. Silicon chip was glue bonded to polymer microfluidic part, which had microfluidic channels as fluid reservoirs and actuation membranes for a fluidic control. Phosphatidylcholine (PC) and phosphoethanolamine (PE) lipids were successfully extracted as biomarkers for breast cancer. &#13;\n&#13;\nMicrochip capillary electrophoresis (CE) was shown on silicon-glass chip, which had integrated thin film electrodes for high voltages (HV). This silicon-glass microchip was used for testosterone measurements with LIF detection. Another CE chip was made by hot embossing PMMA (polymethyl methacrylate). It was used to an antibiotic resistance measurement with mecA gene. Confocal four-colour laser induced fluorescence detection system was developed for roll-to-roll hot embossed PMMA chip. &#13;\n&#13;\nGlass chip was developed for human embryonic stem cells stimulation. Titanium thin film electrodes were deposited on glass surface. The native beat cycle of embryonic stem cells could be altered from about 1 s to 800 ms with electrical stimulation. &#13;\n&#13;\nMicrofluidics makes fast healthcare analysis possible in doctor's office or even in home without time-consuming laboratory analysis. Treatment and medication could be started immediately without delay also avoiding unnecessary medication. Microfluidic devices require small sample amounts and low reagent consumption, which can lower cost of diagnosis; and tightly localized diagnosis and treatment, even at single cell level, enabling novel treatments. This is expected to bring major savings for healthcare system in future.
Safety of a feed additive consisting of a tincture derived from Verbascum thapsus L. (great mullein tincture) for use in all animal species (MANGHEBATI SAS) (2021)

The tincture derived from <i>Verbascum thapsus</i> L. (great mullein tincture) is intended to be used as a sensory additive in feed for all animal species. The product is a water/ethanol solution, with a dry matter content of ˜ 2.8% and contains on average 0.216% polyphenols including 0.093% flavonoids. According to a previous assessment, the additive was not characterised in full and about 82% of the dry matter fraction remained uncharacterised (representing 2.26% of the tincture). There was also uncertainty on the potential presence of iridoid glycosides in the tincture. Therefore, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) could not conclude on the safety of the additive at the proposed use levels of up to 50 g/kg complete feed for all animal species or for the consumer. The applicant has provided new data which show that the unidentified fraction consists of crude fibre, other carbohydrates, and protein. The tincture also contains aucubin (0.004%). Considering the genotoxic potential of aucubin and other related iridoids, no conclusions can be drawn for long-living animals (pets and other non-food producing animals, horses and animals for reproduction). For short-living animals (animals for fattening), the FEEDAP Panel concludes that the tincture is safe at the maximum proposed use level of 50 mg/kg complete feed and that the use in water for drinking is safe provided that the total daily intake of the additive does not exceed the daily amount that is considered safe when consumed via feed. No safety concerns would arise for the consumer from the use of the tincture up to the highest safe level in animal nutrition. In the absence of data, no conclusions can be drawn on the potential of the tincture to be a dermal/eye irritant or a skin sensitiser.
ChemInform Abstract: Oxidative Addition of Thiocyanate to Coordinatively Unsaturated Trinuclear Clusters: Structures of (Pt3(SCN)(μ3‐CO)(μ‐dppm)3)+ and (Pd3(μ3‐S)(CN)(μ‐dppm)3)+ (dppm = Ph2PCH2PPh2). (1987)

Abstract The reaction of the cationic clusters (II) with excess KSCN leads in the case of M:Pt to the title cluster (I) isolated as its PF 6 salt and in the case of M:Pd to rearrangement of the analogous intermediate with cleavage of the S‐C bond of the SCN ion and CO elimination giving the title cluster (III).