gebhart/patrepeval huggingface dataset:
Research on thin film anticounterfeiting coatings at the National Research Council of Canada (1989)

After a brief description of the threats which face the issuers of documents and valuable papers, the concept of thin film anticounterfeiting coatings proposed by the NRCC is explained. The colorimetric considerations which govern the design of such devices are reviewed. Typical coating designs suitable for use in transmission and reflection are discussed. Reflection coatings can be constructed with and without an integral black absorber coating. The need for geometric patterns in the anticounterfeiting coating is explained. Various methods for producing such logos are proposed. Mention is made of the various numerical tools developed for the design and investigation of single films and multilayers, their desensitization to construction errors, and for the investigation of different process control strategies. During the duration of the project, experiments were performed on the optical constants and nonoptical properties of a number of metal and dielectric films considered for use in the anticounterfeiting coatings. These and some of the processes and equipment investigated during the development of a prototype production facility are reviewed. Finally, some security and economic aspects of the devices are considered.
Characterization of the oligosaccharides assembled on the Pichia pastoris--expressed recombinant aspartic protease (1999)

Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combination of mass spectrometric, 2D chromatographic, chemical and enzymatic methods. The carbohydrates from site I (Asn79) were found to range from Man6-17GlcNAc2 with 50% bearing a phospho-diester-motif, site II (Asn113) was not occupied and site III (Asn188) contained mostly uncharged species ranging from Man-13GlcNAc2. These charged groups are not affecting the transport through the secretion pathway of the recombinant glycoprotein. Changes from a molasses-based medium to a minimal salts-based medium led to a clear reduction of the degree of phosphorylation of the N-glycan population.
Rotavirus Vaccine Impact Within an Integrated Healthcare Delivery System in the United States (2022)

We assessed rotavirus vaccine impact using data on acute gastroenteritis (AGE) encounters within an integrated healthcare delivery system during 2000-2018. Following rotavirus vaccine introduction, all-cause AGE rates among children <5 years declined by 36% (95% confidence interval [CI]: 32%-40%) for outpatient and 54% (95% CI: 46%-60%) for inpatient encounters.
CALCULATION OF VOLUMETRIC MATERIAL LOSS FROM TAPER-TRUNNION JUNCTIONS: DEVELOPMENT AND VALIDATION OF A QUANTITATIVE METHOD (2018)

IntroductionIn total joint replacement devices, material loss from the taper junctions is a clinical concern. Previous studies of explanted orthopedic devices have relied on visual scoring methods to quantify the fretting-corrosion damage on the component interfaces. Previous research has shown that visual fretting-corrosion evaluation is correlated to the volume of material loss [1], but scoring is semi-qualitative and does not provide a quantitative measure of the amount of material removed from the surface. The purpose of this study was to develop and validate a quantitative method for measuring the volume of material lost from the surfaces of explanted devices at the taper-trunnion junction.Methods10 new exemplar taper adapter sleeves (Ceramtec, Plochingen, Germany) were used for method validation. By using exemplar devices we were able to create clinically realistic taper damage in a controlled and repeatable manner using machining tools. Taper surfaces were measured before and after in vitro materia...
Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation (2023)

Most cardiac arrest (CA) survivors experience varying degrees of neurologic deficits. To understand the mechanisms that underpin CA-induced brain injury and, subsequently, develop effective treatments, experimental CA research is essential. To this end, a few mouse CA models have been established. In most of these models, the mice are placed in the supine position in order to perform chest compression for cardiopulmonary resuscitation (CPR). However, this resuscitation procedure makes the real-time imaging/monitoring of brain physiology during CA and resuscitation challenging. To obtain such critical knowledge, the present protocol presents a mouse asphyxia CA model that does not require the chest compression CPR step. This model allows for the study of dynamic changes in blood flow, vascular structure, electrical potentials, and brain tissue oxygen from the pre-CA baseline to early post-CA reperfusion. Importantly, this model applies to aged mice. Thus, this mouse CA model is expected to be a critical tool for deciphering the impact of CA on brain physiology.
Synthesis of an iodine‐123‐labeled celecoxib analogue: a potential spect agent (2005)

Abstract Celecoxib (4‐[5‐(4‐methylphenyl)‐3‐(trifluoromethyl)‐1H‐pyrazol‐1yl]‐benzenesulfonamide) is an effective inhibitor of the cyclooxygenase‐2 (COX‐2). The synthesis of a no‐carrier‐added iodine‐123‐labeled analogue of celecoxib was accomplished in four steps for potential use in single photon tomography. Copyright © 2005 John Wiley &amp; Sons, Ltd.
Effects of Escherichia coli dnaE antimutator alleles in a proofreading-deficient mutD5 strain (1995)

We have previously isolated seven mutants of Escherichia coli which replicate their DNA with increased fidelity. These mutants were isolated as suppressors of the elevated mutability of a mismatch-repair-defective mutL strain. Each mutant was shown to contain a single amino acid substitution in the dnaE gene product, the alpha (i.e., polymerase) subunit of DNA polymerase III holoenzyme responsible for replicating the E. coli chromosome. The mechanism(s) by which these antimutators exert their effect is of interest. Here, we have examined the effects of the antimutator alleles in a mutD5 mutator strain. This strain carries a mutation in the dnaQ gene, which results in defective exonucleolytic proofreading. Our results show that dnaE mutations also confer a strong antimutator phenotype in this background, the effects being generally much greater than those observed previously in the mutL background. The results suggest that the dnaE antimutator alleles can exert their effect independently of exonucleolytic proofreading activity. The large magnitude of the antimutator effects in the mutD5 background can be ascribed, at least in part, to the (additional) restoration of DNA mismatch repair, which is generally impaired in mutD5 strains because of error saturation. The high mutability of mutD5 strains was exploited to isolate a strong new dnaE antimutator allele on the basis of its ability to suppress the high reversion rate of an A.T-->T.A transversion in this background. A model suggesting how the dnaE antimutator alleles might exert their effects in proofreading-proficient and -deficient backgrounds is presented.
Puzzle Imaging: Using Large-Scale Dimensionality Reduction Algorithms for Localization (2015)

Current high-resolution imaging techniques require an intact sample that preserves spatial relationships. We here present a novel approach, "puzzle imaging," that allows imaging a spatially scrambled sample. This technique takes many spatially disordered samples, and then pieces them back together using local properties embedded within the sample. We show that puzzle imaging can efficiently produce high-resolution images using dimensionality reduction algorithms. We demonstrate the theoretical capabilities of puzzle imaging in three biological scenarios, showing that (1) relatively precise 3-dimensional brain imaging is possible; (2) the physical structure of a neural network can often be recovered based only on the neural connectivity matrix; and (3) a chemical map could be reproduced using bacteria with chemosensitive DNA and conjugative transfer. The ability to reconstruct scrambled images promises to enable imaging based on DNA sequencing of homogenized tissue samples.
Vibrational multipolar analyses in isotropic media: Combining IR, Raman and hyper-Raman spectroscopies (2012)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Vincent Rodriguez; Vibrational multipolar analyses in isotropic media: Combining IR, Raman and hyper-Raman spectroscopies. AIP Conf. Proc. 10 December 2012; 1504 (1): 254–265. https://doi.org/10.1063/1.4771720 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Synthesis and Photochemistry of a New Photolabile Derivative of GABA-Neurotransmitter Release and Receptor Activation in the Microsecond Time Region (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Photochemistry of a New Photolabile Derivative of GABA-Neurotransmitter Release and Receptor Activation in the Microsecond Time RegionKyle R. Gee, Raymond Wieboldt, and George P. HessCite this: J. Am. Chem. Soc. 1994, 116, 18, 8366–8367Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September 1994https://pubs.acs.org/doi/10.1021/ja00097a054https://doi.org/10.1021/ja00097a054research-articleACS PublicationsRequest reuse permissionsArticle Views422Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A method for translating printed documents using a hand-held device (2011)

We have developed a system to translate and interpret printed documents (such as periodicals) using a commercially available mobile device (e.g., a mobile telephone) with an embedded camera. The system comprises an automatic layout analysis tool along with an Optical Character Recognition (OCR) engine and a translation engine. The translation engine combines Rule-Based Machine Translation (RBMT) software, a list of context-sensitive words/phrases, and an encyclopedia for a list of words. We implemented the proposed system on a Nokia N900 smartphone for translating newspaper articles from Spanish to English. Our tests show that the accuracy and speed of the system is mostly influenced by the accuracy and speed of the OCR method used. Our application requires 19.43 MB of memory and no more than 28 MB of dynamic memory. We show the ability to complete the process for roughly 410 words in as little as 65 seconds for high accuracy or 17 seconds for high speed, on average. The energy consumption in both cases is minimal.
An alternative non-proteolytic mechanism may underlie AGel amyloidosis (2019)

"An alternative non-proteolytic mechanism may underlie AGel amyloidosis." Amyloid, 26(sup1), pp. 150–151
Evaluation of an Epitope-Blocking Enzyme-Linked Immunosorbent Assay for the Diagnosis of West Nile Virus Infections in Humans (2009)

An epitope-blocking enzyme-linked immunosorbent assay (b-ELISA) was evaluated for the diagnosis of West Nile virus (WNV) infections in humans. Sera from patients diagnosed with WNV infections from an outbreak in 2003 in Colorado and from patients diagnosed with dengue virus infections from Mexico and Thailand were tested with the b-ELISA. The b-ELISAs were performed using the WNV-specific monoclonal antibody (MAb) 3.1112G and the flavivirus-specific MAb 6B6C-1. Although the WNV-specific b-ELISA was effective in diagnosing WNV infections in humans from Colorado, it was not efficacious for diagnosing WNV infections in serum specimens from Mexico and Thailand. In serum specimens from patients from Colorado, the WNV b-ELISA and the WNV plaque reduction neutralization test showed an overall agreement of 91%. The sensitivity and specificity of the WNV b-ELISA were 89% and 92%, respectively, with a false-positive rate of 5%, based on receiver operating characteristic analysis. In contrast, false-positive rate results in specimens from the countries of Mexico and Thailand, where flaviviruses are endemic, were 79% and 80%, presumably due to the presence of antibodies resulting from previous dengue virus infections in Mexico and/or Japanese encephalitis virus infections or vaccination in Thailand. Thus, in regions where people have experienced previous or multiple flavivirus infections, the use of the b-ELISA for WNV diagnosis is contraindicated.
Searching a Terabyte of Text Using Partial Replication TITLE2 (1999)

The explosion of content in distributed information retrieval (IR) systems requires new mechanisms in order to attain timely and accurate retrieval of unstructured text. In this paper, we investigate using partial replication to search a terabyte of text in our distributed IR system. We use a replica selection database to direct queries to relevant replicas that maintain query effectiveness, but at the same time restricts some searches to a small percentage of data to improve performance and scalability, and to reduce network latency. We first investigate query locality with respect to time and replica size using real logs from THOMAS and Excite. Our evidence indicates that there is sufficient query locality to justify partial replication for information retrieval and partial replication can achieve better performance than caching queries, because the replica selection algorithm finds similarity between non-identical queries, and thus increases observed locality. We then use a validated simulator to compare database partitioning to partial replication with load balancing, and find partial replication is much more effective at decreasing query response time than partitioning, even with {\em fewer} resources, and it requires only modest query locality. We also demonstrate the average query response time under 10 seconds for a variety of work loads with partial replication on a terabyte text database.
The evolution of the broad-spectrum penicillins (1982)

The evolution of the broad-spectrum penicillins Get access Sydney Selwyn Sydney Selwyn Department of Medical Microbiology, Westminster Medical SchoolLondon SW1P 2AR, England Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Antimicrobial Chemotherapy, Volume 9, Issue suppl_B, 1982, Pages 1–10, https://doi.org/10.1093/jac/9.suppl_B.1 Published: 01 August 1982
VERTICAL FLOATING PINS IN OTC ROUTING (1996)

We present a generalized center terminal cell model aid propose ove-th-cell (OTC) routing algorithms, utilizing the new feature of the model, i.e., Vertical Floating Pins (VFP’s). We approach the problem by linear integer programming and by dynamic programming. For further channel density reduction, both approaches have been combined with linear channel pin assignment. Experiments show a total dcnsity rctluction by 45% coinparctl to oth OTC routing algorithms for center terminal cells.
Expression rénale des marqueurs tubulaires proximaux mégaline et cubiline et étude de l’activité enzymatique mitochondriale au cours de la toxicité rénale du ténofovir (2015)

Le ténofovir disoproxil fumarate (TDF) est une molécule antirétrovirale efficace largement utilisée dans le traitement de l’infection par le virus de l’immunodéficience humaine (VIH) et peut être responsable d’insuffisance rénale et de dysfonction tubulaire proximale. L’objectif de cette étude rétrospective était d’analyser l’expression rénale tubulaire proximale de la mégaline et de la cubiline et d’étudier les activités enzymatiques mitochondriales de la cytochrome c oxydase (COX), codée par l’ADN mitochondrial et nucléaire, et de la succinate deshydrogenase (SDH) codée uniquement par l’ADN nucléaire par histoenzymologie au cours de la néphrotoxicité du TDF. Vingt cas ont été identifiés. La durée médiane d’exposition au TDF était de 36 mois. Au moment de la biospie rénale, le débit de filtration glomérulaire estimé median était de 28 mL/min/1,73 m2. Histologiquement, des signes de nécrose tubulaire aiguë de sévérité variable étaient observés. Des pertes d’expression de mégaline et de cubiline diffuses (> 50 % des tubes proximaux) ou focales (15 à 50 % des tubes proximaux) ont été observées dans 9 et 10 cas respectivement comparés à une perte d’expression < 10 % dans les cas contrôles. La présence de mitochondries géantes en microscopie optique était détectée dans 25 % des cas seulement alors que des dysmorphies mitochondriales ont été notées en microscopie électronique dans 4 cas sur les 5 analysés. L’étude histoenzymologique a montré une perte isolée de COX dans les cellules tubulaires proximales alors que l’activité de SDH était normale. Les altérations focales à diffuses de l’expression tubulaire proximale de mégaline et de cubiline observées sont très probablement le témoin d’une dédifférenciation cellulaire comme ce qui a été décrit au cours de certaines tubulopathies proximales héréditaires. De plus, la perte sélective de l’activité de COX montre que le TDF induit une mitochondriopathie acquise qui est le mécanisme essentiel de la tubulotoxicité du TDF. La tubulopathie proximale induite par le TDF est caractérisée par une perte d’expression de mégaline et de cubiline et par une mitochondriopathie acquise.
Chronic Expansive Retroperitoneal Hematoma (2021)

Chronic expansive retroperitoneal hematoma is a hematoma that grows progressively sometime after the original trauma. It can appear in different locations. The mechanism of onset is unclear, but experimental evidence favours an inflammatory cause. For diagnostic imaging, magnetic resonance imaging is more useful than computed tomography (CT). However, it is difficult to differentiate it from other soft tissue tumors, sarcomas, actinomycosis or inflammatory pseudotumors, due to the uneven central uptake. Histopathological analysis of the lesion usually shows a dense fibrous tissue capsule containing numerous old clots without finding clear atypia or signs suggestive of malignancy. The treatment of choice is complete resection of the lesion (including the capsule), as incomplete treatments (such as drainage or curettage of the hematoma contents) may cause recurrence.
DNA methylotype analysis in colorectal cancer (1994)

The methylation status of a gene promoter is considered to be an important mechanism for the development of many tumors, including colorectal cancer. Recent studies have shown that specific patterns of DNA methylation across multiple CpG loci in some human tumors are more informative than the detection of one single CpG locus in tumor genomes. In the present study, multiple CpG methylations of three genes (CDKN2A, DPYD and MLH1) were detected in DNA samples from patients with colorectal cancer using Pyrosequencing(R) technology. The bisulfite-converted DNA was amplified with a nested PCR and five or six CpG loci of each gene were assessed to determine DNA methylotype. Our data showed that 10/49 (20.4%), 6/48 (12.5%) and 14/49 (28.6%) of tumors were methylated with a DNA methylation level >0.2 in CDKN2A, DPYD and MLH1, respectively. Our study indicated a similar DNA methylation level across the multiple CpG loci for all three genes in the methylated tumor DNA samples, demonstrating a dichotomous trait in DNA methylation. The tumor DNA samples had unique DNA methylation patterns, which were high-degree and multiple-site methylation, but the normal DNA samples had no or a low-degree and dispersed single-site methylation. In addition, an inverse correlation in those methylated tumors was observed between DNA methylation and RNA expression for MLH1 (RS=-0.62, P=0.003), but not for CDKN2A and DPYD. In conclusion, distinctive DNA methylotypes exist in colorectal cancer and may depict a distinct biology in apparently homogeneous tumors.
Induction of Aryl Hydrocarbon (Benzo[a]pyrene) Hydroxylase in Liver Cell Culture by Temporary Inhibition of Protein Synthesis (1973)

The microsomal enzyme complex, aryl hydrocarbon (benzo[a]pyrene) hydroxylase, is inducible by benz[a]anthracene in cells cloned from Buffalo rat liver. The enzyme complex can also be induced to high activity, without adding a polycyclic hydrocarbon inducer, by temporarily inhibiting protein synthesis with cycloheximide or puromycin. This increase in enzyme activity is a function of both the degree and the duration of protein synthesis inhibition. It requires RNA synthesis during the period of inhibition of protein synthesis but not subsequent to the removal of the block; and it is prolonged in the presence of actinomycin D, when administered after the release of the block in protein synthesis. Combined exposure to benz[a]anthracene and cycloheximide is followed by an increase in enzyme activity which is greater than the sum of the increases which follow exposure to each compound alone. Qualitatively similar results were observed in four other mammalian cell culture systems. The results suggest two events in the regulation of aryl hydrocarbon hydroxylase induction: the inducer, benz[a]anthracene, acts primarily at the level of transcription, and a labile protein(s) regulates the translation of induction-specific RNA.
The molecular and cell biology of anion transport by bacteria (1992)

This article summarizes the study of anion exchange mechanisms in bacteria. Along with defining at least two different families of anion exchange, an examination of such carrier-mediated antiport reactions has led to techniques that considerably broaden the scope of biochemical methods for examining membrane proteins. Such advances have been exploited to show that anion exchange itself forms the mechanistic base of an entirely new kind of proton pump, one which may shed light on a variety of bacterial events, including methanogenesis. Perhaps most important, the study of exchange provided the final link in a chain of evidence pointing to a structural 'rhythm' that seems to characterize membrane carriers. These three issues--a biochemical tool, a new proton pump, and a common structural rhythm--are briefly examined in the context of their origins in the analysis of bacterial anion exchange.