gebhart/patrepeval huggingface dataset:
Semi-Quantitative, Duplexed qPCR Assay for the Detection of Leishmania spp. Using Bisulphite Conversion Technology (2019)

Leishmaniasis is caused by the flagellated protozoan <i>Leishmania,</i> and is a neglected tropical disease (NTD), as defined by the World Health Organisation (WHO). Bisulphite conversion technology converts all genomic material to a simplified form during the lysis step of the nucleic acid extraction process, and increases the efficiency of multiplex quantitative polymerase chain reaction (qPCR) reactions. Through utilization of qPCR real-time probes, in conjunction with bisulphite conversion, a new duplex assay targeting the 18S rDNA gene region was designed to detect all <i>Leishmania</i> species. The assay was validated against previously extracted DNA, from seven quantitated DNA and cell standards for pan-<i>Leishmania</i> analytical sensitivity data, and 67 cutaneous clinical samples for cutaneous clinical sensitivity data. Specificity was evaluated by testing 76 negative clinical samples and 43 bacterial, viral, protozoan and fungal species. The assay was also trialed in a side-by-side experiment against a conventional PCR (cPCR), based on the Internal transcribed spacer region 1 (ITS1 region). Ninety-seven percent of specimens from patients that previously tested positive for <i>Leishmania</i> were positive for <i>Leishmania spp.</i> with the bisulphite conversion assay, and a limit of detection (LOD) of 10 copies per PCR was achieved, while the LOD of the ITS1 methodology was 10 cells/1000 genomic copies per PCR. This method of rapid, accurate and simple detection of <i>Leishmania</i> can lead to improved diagnosis, treatment and public health outcomes.
Dynamic non-Singleton fuzzy logic systems for nonlinear modeling (1997)

We investigate dynamic versions of fuzzy logic systems (FLSs) and, specifically, their non-Singleton generalizations (NSFLSs), and derive a dynamic learning algorithm to train the system parameters. The history-sensitive output of the dynamic systems gives them a significant advantage over static systems in modeling processes of unknown order. This is illustrated through an example in nonlinear dynamic system identification. Since dynamic NSFLS's can be considered to belong to the family of general nonlinear autoregressive moving average (NARMA) models, they are capable of parsimoniously modeling NARMA processes. We study the performance of both dynamic and static FLSs in the predictive modeling of a NARMA process.
Histogram testing for strength size effect in asphalt mixtures at low temperature (2013)

This paper presents a simple formulation to predict the size effect curve of mean structural strength of the asphalt mixture from the strength histogram obtained on single-size beam specimens under three-point bend tests. This method is based on the weakest link model (WLM), which relates the mean structural strength to the size dependence of the probability distribution of the material strength. The proposed formulation is verified by an extensive experimental work consisting of strength histogram and mean size effect tests on the asphalt mixture at low temperature. The mean strength size effect curve obtained from the WLM is found to be in very good agreement with the experimental mean strength results obtained on the asphalt mixture specimens of different sizes and geometries, providing evidence of the ductile to brittle transition (Type I size effect) of the asphalt mixture strength.
ChemInform Abstract: Synthetic Routes to Different Classes of Natural Products and Analogues Thereof. Synthesis of Hydroxyethylene Isosteric Dipeptides (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A novel rat mesangial matrix protein, MMP-50/100, involved in mesangial glomerulopathies. (1995)

We conclude that MMP-50/100 is an intrinsic component of the mesangial matrix, presumably related to the "classic" mesangial cell. Expression of MMP-50/100 is increased in expanded mesangial matrices during development of glomerular disease. Furthermore, MMP-50/100 appears to be involved in the handling of mesangial immune complexes.
Capturing the Complete Reaction Profile of a C–H Bond Activation (2021)

The activation of C-H bonds requires the generation of extremely reactive species, which hinders the study of this reaction and its key intermediates. To overcome this challenge, we synthesized an iron(III) chloride-pyridinediimine complex that generates a chlorine radical proximate to reactive C-H bonds upon irradiation with light. Transient spectroscopy confirms the formation of a Cl·|arene complex, which then activates C-H bonds on the PDI ligand to yield HCl and a carbon-centered radical as determined by photocrystallography. First-principles molecular dynamics-density functional theory calculations reveal the trajectory for the formation of a Cl·|arene intermediate. Together, these experimental and computational results show the complete reaction profile for the preferential activation of a C-H bond in the solid state.
Etude et développement d'un dispositif acoustique de suivi de l'encrassement des échangeurs de chaleur à plaques (2006)

Thèse de doctorat en Electronique, Université de Valenciennes et du Hainaut-Cambrésis, 5 mai
Large Screen Display Technology Survey. (1984)

Abstract : This document provides information on the technologies of large screen displays which can be used in command and control applications. The present survey incorporates information on existing product line equipment and includes new devices which are just entering the market or are in an advanced stage of development. This material is based upon the results of an extensive survey of manufacturers of large screen display equipment for commercial and military applications. (Author)
ChemInform Abstract: Conductivity of Ion Implanted and Rapid Thermal Annealed Semi‐Insulating Polycrystalline Silicon (1988)

Abstract (SIPOS).
Application of Computational Fluid Dynamics in Reciprocating Compressor Design (2001)

Abstract Heat and mass transfer processes are of paramount importance when designing some important compressor components, specially the ones that perform flow control, noise suppression and the compression process itself. In general, pressure losses, flow pulsation and heat exchanges must be minimized to achieve high efficiencies. On the other hand, the optimization of those parameters are subject to a compromise among noise generation, component manufacturing costs and reliability, hence the bigger is the amount of (correct) information available, the higher is the probability of a successful project. In this scenario, the use of Computational Fluid Dynamics (CFD) plays an important role in the development phase, since it allows the analysis of more options, faster and at lower expenses comparing to the “prototype-and-test” approach. It also spreads possibilities of alternatives prone to be considered in a technical trade-off and anticipates problems and design constrains prior to prototyping. This paper focus on case studies and designing problems solved by the authors using different CFD based approaches. The problems are described in terms of the approach used in the solution, boundary conditions, models adopted and results obtained.
An Ultra-Low Power CMOS Image Sensor with On-Chip Energy Harvesting and Power Management Capability (2015)

An ultra-low power CMOS image sensor with on-chip energy harvesting and power management capability is introduced in this paper. The photodiode pixel array can not only capture images but also harvest solar energy. As such, the CMOS image sensor chip is able to switch between imaging and harvesting modes towards self-power operation. Moreover, an on-chip maximum power point tracking (MPPT)-based power management system (PMS) is designed for the dual-mode image sensor to further improve the energy efficiency. A new isolated P-well energy harvesting and imaging (EHI) pixel with very high fill factor is introduced. Several ultra-low power design techniques such as reset and select boosting techniques have been utilized to maintain a wide pixel dynamic range. The chip was designed and fabricated in a 1.8 V, 1P6M 0.18 µm CMOS process. Total power consumption of the imager is 6.53 µW for a 96 × 96 pixel array with 1 V supply and 5 fps frame rate. Up to 30 μW of power could be generated by the new EHI pixels. The PMS is capable of providing 3× the power required during imaging mode with 50% efficiency allowing energy autonomous operation with a 72.5% duty cycle.
A systematic literature review of the clinical efficacy of repetitive transcranial magnetic stimulation (rTMS) in non-treatment resistant patients with major depressive disorder (2019)

The use of rTMS in patients after ≤1 medication trial should be considered. US payers should consider revising their coverage policies to include the use of rTMS in these patients.
The Optimization of Squared-Off Cascades for Isotope Separation (1994)

The optimization of squared-off cascades for isotope separation is discussed. Criteria are developed for determining the optimal transition concentrations between the square sections of the squared-off cascade. Equations are presented from which the optimal values of the cascade parameters that maximize the separative efficiency of the cascade can be obtained. Numerical results of calculations for cascades producing low-enriched uranium are also presented.
Optical independent-sideband modulation for bandwidth-economic coherent transmission (2014)

We proposed and implemented a bandwidth-economic coherent optical transmission technology using optical independent-sideband (O-ISB) modulation. Generation of two software-defined, 30-GBd O-ISB channels per wavelength sharing one set of transmitter hardware was demonstrated, which was made possible by digital single-sided up-conversion, channel pre-equalization and optical IQ modulation with precise amplitude and delay matching between driving signals. With 120-Gb/s PM-QPSK per O-ISB channel, we successfully delivered 16 O-ISB channels in 8 wavelengths at 3.86-bits/s/Hz over 2100 km SSMF. In addition, the system performance was evaluated by using regular and enhanced Rx DSP, respectively. For metro and regional network applications where small footprint, low power consumption and low cost are required features, the proposed two-channels-per-wavelength O-ISB modulation may be considered an economic option.
Peak to Average Power Ratio Analysis for LTE Systems (2010)

The 3rd generation partnership project (3GPP) long term evolution (LTE) standard uses single carrier frequency division multiple access (SCFDMA) scheme for the uplink transmissions and orthogonal frequency division multiplexing access (OFDMA) in downlink. SCFDMA uses DFT spreading prior to OFDMA modulation to map the signal from each user to a subset of the available subcarriers i.e., single carrier modulation. The efficiency of a power amplifier is determined by the peak to average power ratio (PAPR) of the modulated signal. In this paper, we analyze the PAPR in 3GPP LTE systems using root raised cosine based filter. Simulation results show that the SCFDMA subcarrier mapping has a significantly lower PAPR compared to OFDMA. Also comparing the three forms of SCFDMA subcarrier mapping, results show that interleave FDMA (IFDMA) subcarrier mapping with proposed root raised cosine filter reduced PAPR significantly than localized FDMA (LFDMA) and distributed (DFDMA) mapping. This improves its radio frequency (RF) power amplifier efficiency and also the mean power output from a battery driven mobile terminal.
Surface characterization of oligonucleotides immobilized on polymer surfaces (2002)

The immobilization and hybridization of amino-terminated oligonucleotide strands to cyclo-olefin-copolymer (COC) and polycarbonate (PC) surfaces have been investigated for potential application in micro-PCR devices. The oligonucleotides were covalently bound to the plasma-treated COC and PC surfaces via an N-hydroxy-sulfosuccinimide (NHSS) intermediate. Analysis by AFM showed that the oligonucleotides were present on the surfaces as lumps, and that the size, both vertically and laterally, of these lumps on the COC surface was larger compared to the PC surface. The immobilization efficiency of the former was also higher (15.8 x 10<sup>12</sup> molecules / cm<sup>2</sup>) compared to the latter (3.3 x 10<sup>12</sup> molecules / cm<sup>2</sup>). The higher efficiency of the COC surface is attributed to the more effective NHSS-functionalization and its higher surface roughness. Subsequent hybridization doubled the height of the lumps, while the lateral dimensions remained essentially unchanged. This is explained in terms of organization of the long probe strands used on the surface as flexible, coil-like polymer chains, which allow the complementary oligonucleotides to bind and increase the height of the lumps. The AFM frictional images showed that the hybridization had the effect of reversing hydrophilicity of the oligonucleotide lumps from being more hydrophilic to more hydrophobic, consistent with the hydrophilic bases of the probe strands being shielded as a result of hybridization.
New Heteroaromatic Compounds. VII. Chloro and Bromo Derivatives of 10-Hydroxy-10,9-borazarophenanthrene (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Heteroaromatic Compounds. VII. Chloro and Bromo Derivatives of 10-Hydroxy-10,9-borazarophenanthreneMICHAEL J. S. DEWAR and VED P. KUBBACite this: J. Org. Chem. 1960, 25, 10, 1722–1724Publication Date (Print):October 1, 1960Publication History Published online1 May 2002Published inissue 1 October 1960https://pubs.acs.org/doi/10.1021/jo01080a010https://doi.org/10.1021/jo01080a010research-articleACS PublicationsRequest reuse permissionsArticle Views197Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Dexamethasone Diffusion Across Contact Lenses Is Inhibited by Staphylococcus epidermidis Biofilms in Vitro (2014)

The results of this in vitro study demonstrate that bacterial biofilms impede dexamethasone diffusion through silicone hydrogel contact lenses and warrant future studies regarding the clinical benefit of using ocular therapeutics in the setting of bandage contact lens use for corneal epithelial defects.
Effects of ultraviolet radiation on skin structure and ultrastructure in Caspian Sea Salmon,Salmo trutta caspius, during alevin stage (2010)

Ozone reduction enhances the amount of ultraviolet (UV) radiation reaching the aquatic ecosystems, which can induce adverse effects on fish. In order to study the UV radiation effects on skin structure and ultrastructure, the alevins of Salmo trutta caspius were exposed to two different doses of UV radiation (low doses of: 60 µW cm−2 UVC; 100 µW cm−2 UVB; and 40 µW cm−2 UVA and high doses of: 90 µW cm−2 UVC; 130 µW cm−2 UVB; and 50 µW cm−2 UVA) for 15 min once a day in dark condition. Alevins with no exposure to either solar light or UV exposure served as the control group. No mortality was observed in the control group, whereas all of the alevins died in the 9th day in both UV exposure groups. Light microscopic and scanning electron microscopic studies showed significant reduction in the epidermis thickness, number of skin mucous cells, and percentage of mucus secreting cells in both UV exposure groups. Sunburn cells, lifting epidermis from basal membrane, and disappearance of pavement cell microridges were also observed. Our results showed that UV radiation caused intensive skin cell lesions in the Caspian salmon alevins. Since the skin cells of trout alevins possesses essential roles in respiration, osmoregulation, excretion and defense, the observed damages to the skin could be attributed to their sudden mortality in the UV exposure.
S100B Blood Levels Correlate with Rewarming Time and Cerebral Doppler in Pediatric Open Heart Surgery (2010)

The present results show a significant correlation between a biochemical marker of brain damage and an index of increased cerebrovascular resistance, with higher levels during the rewarming CPB phase in pediatric open heart surgery.
Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens (2020)

Optical imaging of the dynamics of living specimens involves tradeoffs between spatial resolution, temporal resolution, and phototoxicity, made more difficult in three-dimensions. Here, however, we report that rapid 3D dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultra-thin planar illumination produced by scanned Bessel beams with superresolution structured illumination microscopy. We demonstrate in vivo karyotyping of chromosomes during mitosis and identify different dynamics for the actin cytoskeleton at the dorsal and ventral surfaces of fibroblasts. Compared to spinning disk confocal microscopy, we demonstrate substantially reduced photodamage when imaging rapid morphological changes in D. discoideum cells, as well as improved contrast and resolution at depth within developing C. elegans embryos. Bessel beam structured plane illumination thus promises new insights into complex biological phenomena that require 4D subcellular spatiotemporal detail in either a single or multicellular context.
AAV Vector Mediated Delivery of NG2 Function Neutralizing Antibody and Neurotrophin NT-3 Improves Synaptic Transmission, Locomotion, and Urinary Tract Function after Spinal Cord Contusion Injury in Adult Rats (2023)

NG2 is a structurally unique transmembrane chondroitin sulfate proteoglycan (CSPG). Its role in damaged spinal cord is dual. NG2 is considered one of key inhibitory factors restricting axonal growth following spinal injury. Additionally, we have recently detected its novel function as a blocker of axonal conduction. Some studies, however, indicate the importance of NG2 presence in the formation of synaptic contacts. We hypothesized that the optimal treatment would be neutralization of inhibitory functions of NG2 without its physical removal. Acute intraspinal injections of anti-NG2 monoclonal antibodies reportedly prevented an acute block of axonal conduction by exogenous NG2. For prolonged delivery of NG2 function neutralizing antibody, we have developed a novel gene therapy: adeno-associated vector (AAV) construct expressing recombinant single-chain variable fragment anti-NG2 antibody (AAV-NG2Ab). We examined effects of AAV-NG2Ab alone or in combination with neurotrophin NT-3 in adult female rats with thoracic T10 contusion injuries. A battery of behavioral tests was used to evaluate locomotor function. <i>In vivo</i> single-cell electrophysiology was used to evaluate synaptic transmission. Lower urinary tract function was assessed during the survival period using metabolic chambers. Terminal cystometry, with acquisition of external urethral sphincter activity and bladder pressure, was used to evaluate bladder function. Both the AAV-NG2Ab and AAV-NG2Ab combined with AAV-NT3 treatment groups demonstrated significant improvements in transmission, locomotion, and bladder function compared with the control (AAV-GFP) group. These functional improvements associated with improved remyelination and plasticity of 5-HT fibers. The best results were observed in the group that received combinational AAV-NG2Ab+AAV-NT3 treatment.<b>SIGNIFICANCE STATEMENT</b> We recently demonstrated beneficial, but transient, effects of neutralization of the NG2 proteoglycan using monoclonal antibodies delivered intrathecally via osmotic mini-pumps after spinal cord injury. Currently, we have developed a novel gene therapy tool for prolonged and clinically relevant delivery of a recombinant single-chain variable fragment anti-NG2 antibody: AAV-rh10 serotype expressing scFv-NG2 (AAV-NG2Ab). Here, we examined effects of AAV-NG2Ab combined with transgene delivery of Neurotrophin-3 (AAV-NT3) in adult rats with thoracic contusion injuries. The AAV-NG2Ab and AAV-NG2Ab+AAV-NT3 treatment groups demonstrated significant improvements of locomotor function and lower urinary tract function. Beneficial effects of this novel gene therapy on locomotion and bladder function associated with improved transmission to motoneurons and plasticity of axons in damaged spinal cord.
Targeting Human Medulloblastoma: Oncolytic Virotherapy with Myxoma Virus Is Enhanced by Rapamycin (2007)

We have shown previously the oncolytic potential of myxoma virus in a murine xenograft model of human glioma. Here, we show that myxoma virus used alone or in combination with rapamycin is effective and safe when used in experimental models of medulloblastoma in vitro and in vivo. Nine of 10 medulloblastoma cell lines tested were susceptible to lethal myxoma virus infection, and pretreatment of cells with rapamycin increased the extent of in vitro oncolysis. Intratumoral injection of live myxoma virus when compared with control inactivated virus prolonged survival in D341 and Daoy orthotopic human medulloblastoma xenograft mouse models [D341 median survival: 21 versus 12.5 days; P = 0.0008; Daoy median survival: not reached (three of five mice apparently "cured" after 223 days) versus 75 days; P = 0.0021]. Rapamycin increased the extent of viral oncolysis, "curing" most Daoy tumor-bearing mice and reducing or eliminating spinal cord and ventricle metastases. Rapamycin enhanced tumor-specific myxoma virus replication in vivo and prolonged survival of D341 tumor-bearing mice (median survival of mice treated with live virus (LV) and rapamycin, versus LV alone, versus rapamycin alone, versus inactivated virus: 25 days versus 19, 13, and 11 days, respectively; P < 0.0001). Rapamycin increased the levels of constitutively activated Akt in Daoy and D341 cells, which may explain its ability to enhance myxoma virus oncolysis. These observations suggest that myxoma virus may be an effective oncolytic agent against medulloblastoma and that combination therapy with signaling inhibitors that modulate activity of the phosphatidylinositol 3-kinase/Akt pathway will further enhance the oncolytic potential of myxoma virus.