gebhart/patrepeval huggingface dataset:
Novel Insights into Regulation of Asparagine Synthetase in Conifers (2012)

Asparagine, a key amino acid for nitrogen storage and transport in plants, is synthesized via the ATP-dependent reaction catalyzed by the enzyme asparagine synthetase (AS; EC 6.3.5.4). In this work, we present the molecular analysis of two full-length cDNAs that encode asparagine synthetase in maritime pine (Pinus pinaster Ait.), PpAS1, and PpAS2. Phylogenetic analyses of the deduced amino acid sequences revealed that both genes are class II AS, suggesting an ancient origin of these genes in plants. A comparative study of PpAS1 and PpAS2 gene expression profiles showed that PpAS1 gene is highly regulated by developmental and environmental factors, while PpAS2 is expressed constitutively. To determine the molecular mechanisms underpinning the differential expression of PpAS1, the promoter region of the gene was isolated and putative binding sites for MYB transcription factors were identified. Gel mobility shift assays showed that a MYB protein from Pinus taeda (PtMYB1) was able to interact with the promoter region of PpAS1. Furthermore, transient expression analyses in pine cells revealed a negative effect of PtMYB1 on PpAS1 expression. The potential role of MYB factors in the transcriptional regulation of PpAS1 in vascular cells is discussed.
Optimizing the Extraction Conditions of Hydroxytyrosol from Olive Leaves Using a Modified Spherical Activated Carbon: A New Experimental Design (2022)

The purification of hydroxytyrosol from olive leaves extract by modified activated carbon was studied experimentally in a batch system and a column by adsorption and desorption processes. The extraction yield reached 90% of hydroxytyrosol, which is the major compound found in the extract. Despite the abundance of research on extracts of hydroxytyrosol from olive leaves, it seems that the applied methods can be further improved. In this study, several approaches were applied to optimize the extraction conditions of this molecule. Hence, the response surface method and the Box-Behnken design (BBD) were used to evaluate the effect of the temperature, time, and adsorbent dose on the hydroxytyrosol recovery. Moreover, adsorption isotherm, kinetics, and thermodynamic studies were also performed to clarify the nature of the process. The main finding was the obtainment of a maximum adsorption yield of 97.5% at an adsorbent/adsorbate ratio of 1 : 20, after a 6 h cycle and at a temperature of 30°C. Furthermore, adsorption process seemed to fit best with Freundlich model. In addition, the thermodynamic study describes a spontaneous and endothermic process. Desorption assay using ethanol helped to recover 73% of hydroxytyrosol. Furthermore, the HPLC analysis of fractions after column adsorption showed a simple peak of hydroxytyrosol with purity higher than 97% and a flavonoids-rich fraction. These findings would indicate that this separation method for the recovery of phenolic compounds with high antioxidant activity can be a very promising one.
SERIES PARTS MANUFACTURING BY INFRARED SUPERPLASTIC FORMING WITH NUMERICAL TECHNIQUES FOR THERMAL REGULATION (2020)

Materials used for Superplastic forming (SPF) are mainly titanium alloys which are good candidates to produce lightweight complex-shaped components for high performance aerospace applications. SPF process has limitations because it involves a high-temperature furnace with poor heat efficiency and expensive tooling with low management flexibility. Enhancing this manufacturing process is a major challenge for the aerospace industry which is facing to important production ramp-up and cost reductions. Direct heating combined with tool heat management result in significant savings of SPF process: production time savings by drastically reduce the heating time, reduction of maintenance costs and energy savings by significant heat efficiency improvement. Aurock developed direct heating by Infrared emitters and succeed in forming series 1.5x1m² Ti6Al-4V blanks. A key point with this new technology is to ensure a homogeneous blank temperature all along the forming. This point is achieved thanks to lamp power modulations and numerical techniques to secure the blank thermal regulation with a full radiative flux control at different forming stages. Results obtained are stable and repeatable regarding to dimensional criteria, post-forming thicknesses distribution and microstructure. Numerical predictions are in very good agreement with the experimental results, enabling robust machine setup for series Infrared SPF parts production.
Availability-Aware Routing for Large-Scale Hybrid Wireless-Optical Broadband Access Network (2010)

In large-scale hybrid wireless-optical broadband access networks, the availability of wireless links and optical links varies considerably. Availability-aware routing can significantly improve availability and throughput by encouraging more usage of PON.
Nanotechnology for dental composites (2004)

In dentistry, composites consisting of inorganic fillers such as radiopaque glass, quartz or ceramic particles and an organic resin matrix based on a mixture of dimethacrylates, are used for the restoration of teeth. Important properties of the dental restorative materials can be improved by means of nanotechnology. Inorganic-organic hybrid materials can be used as monomer matrix in dental restoratives to diminish their polymerisation shrinkage and improve their wear resistance and biocompatibility. Inorganic-organic hybrids with tailor-made properties can be created by means of sol-gel processing of hydrolytically condensable, organically modified trialkoxysilanes, which contain radically polymerisable methacrylate groups or cyclic groups capable of ring-opening polymerisation. Furthermore, nanofillers can contribute to increasing the modulus of elasticity or improving the optical properties of the dental composites and are useful as starting compounds for the synthesis of new low temperature processable dental ceramics. Moreover, well designed nano- and microstructured sol-gel components can be used for producing protective and wear resistant coatings for teeth, metal alloys, and glass fillers of special compositions.
A Closed-form Solution to Photorealistic Image Stylization (2018)

Photorealistic image stylization concerns transferring style of a reference photo to a content photo with the constraint that the stylized photo should remain photorealistic. While several photorealistic image stylization methods exist, they tend to generate spatially inconsistent stylizations with noticeable artifacts. In this paper, we propose a method to address these issues. The proposed method consists of a stylization step and a smoothing step. While the stylization step transfers the style of the reference photo to the content photo, the smoothing step ensures spatially consistent stylizations. Each of the steps has a closed-form solution and can be computed efficiently. We conduct extensive experimental validations. The results show that the proposed method generates photorealistic stylization outputs that are more preferred by human subjects as compared to those by the competing methods while running much faster. Source code and additional results are available at https://github.com/NVIDIA/FastPhotoStyle .
MONOCLONAL ANTIBODIES AGAINST SYNTHESIZED SHORT PEPTIDES CORRESPONDING TO HUMAN AA AMYLOID PROTEIN (1987)

Monoclonal antibodies (McAbs) were raised against the synthesized short peptides corresponding to 37-47 residues in amino acid sequence of human AA protein. The McAbs reacted immunohistochemically to amyloid tissues from cow, mouse, swan, and human AA amyloidosis. We concluded that the McAbs were useful for identification of AA type amyloidosis of various species, and that the 37-47 residues were effective antigenic sites in AA protein.
Theoretical investigation of asymmetric methyl rotor dynamics in partially deuterated acetophenones (1992)

A b initio molecular orbital calculations at the HF/4-21G level, are reported of the structure, the molecular force field, and the methyl torsional potential energy curve of acetophenone in an attempt to interpret the observed tunneling and rotamer splittings in the S0→T(nπ*) excitation spectra of methyl-deuterated acetophenone-d1 and -d2. It is found that the angular dependence of the torsional kinetic energy operator does not contribute significantly to the rotamer splittings, which instead are mainly due to the angular dependence of the overall zero-point energy. The calculated zero-point energy differences between the two rotamers in the ground state are 14 cm−1 for acetophenone-d1 and 12 cm−1 for -d2. To account for the observed rotamer splittings of 7.5 and 4 cm−1, respectively, the corresponding zero-point energy difference in the triplet state must be about 9 cm−1 for both isotopomers. Comparison with experimental results for isotopomers of acetaldehyde indicates that these values are reasonable and support the notion that the level of theory used yields relevant results in the case at hand. The similarity of the zero-point difference between the rotamers in the ground and excited state contrasts strongly with the large difference in torsional barrier: 700–800 cm−1 for the ground state and 95 cm−1 for the triplet state of acetophenone-d0; the latter value is derived from the tunneling splitting observed in the excitation spectrum. These contrasting results are due to the fact that the barrier is an electronic effect whereas the rotamer splitting is a purely vibrational effect.
Understanding patients (2002)

The objective of our research was to undertake first steps to analyse patient access to their electronic health records (EHR) as a crucial universal access issue: Why is patient involvement becoming a key issue, what approaches are available to learn more about patient attitudes and needs, which concrete outcomes can be obtained from such research? The paper outlines a reference scenario for tele home monitoring of chronically ill patients including measurement devices and system environment, provides an assessment of selected participatory approaches like questionnaires, interviews and group discussions, and reports about universal access design issues from a patient perspective. Concrete conclusions concerning access devices and presentation of EHR contents are developed. To allow all citizens equality in access, to benefit from advances in eHealth and to avoid a "Medical Divide", creativity, innovations and support are needed to progress towards a true Information Society for all also in the health arena.
Determination of the normal contact stiffness and integration time step for the finite element modeling of bristle-surface interaction (2018)

In finite element modeling of impact, it is necessary to define appropriate values of the normal contact stiffness, Kn, and the Integration Time Step (ITS). Because impacts are usually of very short duration, very small ITSs are required. Moreover, the selection of a suitable value of Kn is a critical issue, as the impact behavior depends dramatically on this parameter. In this work, a number of experimental tests and finite element analyses have been performed in order to obtain an appropriate value of Kn for the interaction between a bristle of a gutter brush for road sweeping and a concrete surface. Furthermore, a suitable ITS is determined. The experiments consist of releasing a steel bristle that is placed vertically at a certain distance from a concrete surface and tracking the impact. Similarly, in the finite element analyses, a beam is modeled in free fall and impacting a surface; contact and target elements are attached to the beam and the surface, respectively. The results of the experiments and the modeling are integrated through the principle of conservation of energy, the principle of linear impulse and momentum, and Newton’s second law. The results demonstrate that, for the case studied, Kn and the impact time tend to be independent of the velocity just before impact and that Kn has a very large variation, as concrete is a composite material with a rough surface. Also, the ratio between the largest height of the bristle after impact and the initial height tends to be constant.
Real and imagined barriers to an electronic medical record. (1993)

We developed an electronic medical record for ambulatory patients as part of the integrated clinical information system at Beth Israel Hospital. During the four years since it was installed, clinicians have entered 76,060 patient problems, 137,713 medications, and 33,938 notes. Residents, who had to type notes in themselves, entered 49.5% of their notes into OMR. Several factors that we had predicted would be barriers to an electronic medical record, such as clinician reluctance to type or perform data entry, have not proved to be significant problems. Other anticipated barriers, such as difficulties with dual charting on paper during transition to an electronic medical record, have been realized. The major unexpected barrier that has been encountered is increased clinician concern about the privacy and security of full text notes relative to other data elements in the clinical information system. We have attempted to modify the electronic medical record so as to overcome some of these barriers.
Aarskog Syndrome (2010)

This chapter contains sections titled: Introduction Manifestations and Management Resources
Intrahost variation and evolutionary dynamics of adenoviruses correlate to neutrophils in infected patients (2022)

With deep sequencing of virus genomes within the hosts, intrahost single nucleotide variations (iSNVs) have been used for analyses of virus genome variation and evolution, which is indicated to correlate with viral pathogenesis and disease severity. Little is known about the features of iSNVs among DNA viruses. We performed the epidemiological and laboratory investigation of one outbreak of adenovirus. The whole genomes of viruses in both original oral swabs and cell-cultured virus isolates were deeply sequenced. We identified 737 iSNVs in the viral genomes sequenced from original samples and 46 viral iSNVs in cell-cultured isolates, with 33 iSNVs shared by original samples and cultured isolates. Meanwhile, we found these 33 iSNVs were shared by different patients, among which, three hot spot areas 6367-6401, 9213-9247, and 10 584-10 606 within the functional genes of the adenovirus genome were found. Notably, the substitution rates of iSNVs were closely correlated with the clinical and immune indicators of the patients. Especially a positive correlation to neutrophils was found, indicating a predictable biomarker of iSNV dynamics. Our findings demonstrated the neutrophil-correlated dynamic evolution features of the iSNVs within adenoviruses, which indicates a virus-host interaction during human infection of a DNA virus.
Cobalt-substituted SrTi0.3Fe0.7O3−δ: a stable high-performance oxygen electrode material for intermediate-temperature solid oxide electrochemical cells (2018)

SrTi<sub>0.3</sub>Fe<sub>0.7−x</sub>Co<sub>x</sub>O<sub>3−δ</sub>oxygen electrodes provide a unique combination of low polarization resistance and stability useful for solid oxide electrochemical cells.
MIPS: The Model-Checking Integrated Planning System (2001)

Mips is a planning system that applies binary decision diagrams (BDDs) to compactly represent world states in a planning problem and efficiently explore the underlying state space. It was the first general planning system based on model-checking methods. It can handle the strips subset of the pddl language and some additional features from adl, namely, negative preconditions and (universal) conditional effects. At the Fifth International Conference on Artificial Intelligence Planning and Scheduling (AIPS'00), mips was one of five planning systems to be awarded for distinguished performance in the fully automated track. This article gives a brief introduction to, and explains the basic planning algorithm used by, mips, using a simple logistics problem as an example.
MySurgeryRisk and Machine Learning (2018)

Annals of Surgery, the world's most highly referenced surgery journal, provides the international medical community with information on significant contributions to the advancement of surgical science and practice. Surgeons look to Annals of Surgery first for innovations in practice and technique. Special editorial features include "Advances in Surgical T​echnique," which provides up-to-the-minute coverage of current clinical issues, and monthly review articles that cover the most current practice concerns. Affiliated Societies: American Surgical Association European Surgical Association
Cyclin-dependent kinase regulates the length of S phase through TICRR/TRESLIN phosphorylation (2015)

S-phase cyclin-dependent kinases (CDKs) stimulate replication initiation and accelerate progression through the replication timing program, but it is unknown which CDK substrates are responsible for these effects. CDK phosphorylation of the replication factor TICRR (TopBP1-interacting checkpoint and replication regulator)/TRESLIN is required for DNA replication. We show here that phosphorylated TICRR is limiting for S-phase progression. Overexpression of a TICRR mutant with phosphomimetic mutations at two key CDK-phosphorylated residues (TICRR(TESE)) stimulates DNA synthesis and shortens S phase by increasing replication initiation. This effect requires the TICRR region that is necessary for its interaction with MDM two-binding protein. Expression of TICRR(TESE) does not grossly alter the spatial organization of replication forks in the nucleus but does increase replication clusters and the number of replication forks within each cluster. In contrast to CDK hyperactivation, the acceleration of S-phase progression by TICRR(TESE) does not induce DNA damage. These results show that CDK can stimulate initiation and compress the replication timing program by phosphorylating a single protein, suggesting a simple mechanism by which S-phase length is controlled.
Pulse polarization splitting in a transient wave plate (2006)

We demonstrate propagation of ultrafast laser pulses through a molecular gas acting as a transient wave plate under conditions of strong phase modulation. The resulting decomposition of a linearly polarized laser pulse into two nearly distinct, orthogonally polarized laser pulses is demonstrated with transiently aligned, linear molecules.
Super-wetting, wafer-sized silicon nanowire surfaces with hierarchical roughness and low defects (2012)

This paper reports the fabrication of wafer-sized silicon nanowire (SiNW) surfaces using a modified metal-assisted chemical etching method. The complete fabrication and coating process can be performed in less than three hours, is easily size-scalable, and produces surfaces with very low surface defects, complex, hierarchical surface roughness, and large nanowire height. These surfaces exhibit extreme wettabilities depending on surface coating: oxidized SiNW surfaces are superhydrophilic, while surfaces coated with a fluorinated hydrocarbon are superhydrophobic. The wetting and morphological properties of SiNW surfaces made with one and two etches of different duration are characterized vis-à-vis their effect on water drop mobility. Compared to a single etch process, a double etch followed by coating with a fluorinated hydrocarbon more efficiently produces SiNW surfaces with high contact angles on which microliter-sized water drops roll-off at approximately 0° tilt angle. Due to their very low friction, extreme wetting properties, ease of fabrication, low-cost, and large-sizes, these SiNW surfaces may be advantageous in microfluidic devices, bioanalysis systems sensitive to cross-contamination that require disposable substrates, and other applications.
Development of a design catalog for bonded concrete overlays in the regional road network (2022)

This paper describes the development of a design catalog for the design of bonded concrete overlays (whitetopping) for existing asphalt roads in the regional road network of Austria. For the underlying calculations of stresses and strains due to traffic loads, different finite-element models with multiple layers were used. Thereby, a large variety of important input parameters were taken into account, such as the condition of the existing pavement, described by its residual bearing capacity. The influence of different climatic conditions over the course of a year to the material properties of the pavement-layers (Young’s moduli) and the bonding properties between the existing asphalt and the new concrete layer were also considered. The calculation of stresses due to temperatur loads (curling stresses) were estimated by using the revised Eisenmann’s model [1]. Based on the determined stresses in the concrete layer due to traffic and temperature loads, a new performance-based design method was used for the implementation of a proper failure criterion and the calculation of the technical service life of the modelled whitetopping pavements. In a further step, the required layer thicknesses of the concrete overlay were determined, depending on the length and the width of the concrete slabs as well as the residual bearing capacity and the remaining thickness of the existing asphalt layers. This allowed the development of different proposed designs for whitetopping-pavements, each with defined layer thicknesses, which were finally summarized into a design catalog. The development of this design catalog and the usage of the proposed pavements will enable a simple but realistic design of whitetopping-overlays on existing asphalt pavements.
Sentiment Analysis Of Journal Articles, Press Releases, And News Articles Pertaining To Chronic Traumatic Encephalopathy (2020)

PURPOSE: Previous research has called for media balance when reporting on chronic traumatic encephalopathy (CTE) in order to avoid harmful bias towards readers. This call is raised from concerns that the media’s representation of CTE has moved beyond what science has proven. The purpose of this study was to use word sentiments to directly compare journal articles with corresponding news articles to evaluate these concerns. The news articles were split into three groups: press releases reporting the articles’ findings, news articles about CTE from upper tier news outlets, and articles from lower tier news outlets. METHODS: Research articles (n=10) directly associated with CTE that were heavily covered in the media were selected for this sample. An equivalent number of press releases (n=10), upper tier articles (n=10), and lower tier articles (n=10) were collected in order to compare semantics. The “AFINN” sentiment analysis dictionary rates the emotional valence of each word with an integer between minus three (negative connotation) and plus three (positive connotation). Words not recognized by the dictionary or with a zero weight were omitted from the analyses. Mean sentiment score was adjusted for total word count. RESULTS: The mean sentiment scores, adjusted were words count, were as follows: 0.086 for journal articles, -0.096 for press releases, -0.122 for upper tier sources, and 0.026 for lower tier sources. An analysis of variance calculation yielded no significant differences between the groups (F = 1.058, p = 0.379). CONCLUSIONS: Despite recent calls for a less biased reporting of CTE in mainstream media, our analysis indicates essentially equal sentimental weighting between peer-reviewed journal articles and news reports on CTE, whether the report was a press release, an article from an upper tier source, or from a lower tier source. Additionally, these sentiment weights each approached a value of zero (true neutrality). Future research should take into account the context in which the words appears in the articles in addition to using sentiment averages.