gebhart/patrepeval huggingface dataset:
How a tarantula can help treat infections: Avicularia juruensis ’s theraphotoxins that also act as antimicrobials (2022)

Short abstract Considering that there are still many species little-studied, this work aimed to analyze the venom of the spider Avicularia juruensis searching for antimicrobial peptides. Using reverse-phase high-performance liquid chromatography, microbial growth inhibition assay, transcriptomics, and proteomics approaches we identified three antimicrobial peptides: Avilin, Juruin_2, and Juruenine. All of them showed similarities with neurotoxins that act on ion channels and, probably, they have the ICK motif. The study of animal venoms is of great importance to carry out the characterization of unknown components and that may have a biotechnological application, in special venoms from spiders that are from less studied families. Spiders are the most successful group of venomous animals, comprising more than 50,350 species distributed in all terrestrial habitats. One strategy that facility their broad distribution is the production of elaborate venoms, which are composed of inorganic salts, organic molecules with low molecular mass, free amino acids, small polypeptides, linear peptides, nucleotides, disulfide-rich peptides, enzymes, and high molecular mass proteins. Considering that there are still many species little-studied, this work aimed to analyze the venom of the mygalomorph spider Avicularia juruensis searching for new antimicrobial peptides. Using reverse-phase high-performance liquid chromatography, microbial growth inhibition assay, transcriptomics, and proteomics approaches we identified three antimicrobial peptides that were named Avilin, Juruin_2, and Juruenine. All of them showed similarities with neurotoxins that act on ion channels and, probably, they have the ICK motif in their structure. The ICK fold seems to be conserved in several venomous animal lineages and presents elevated functional diversity, as well as gives stability to the toxins. The study of animal venoms is of great importance to carry out the characterization of unknown components and that may have a biotechnological application (like the antimicrobial peptides), in special venoms from spiders that are from less studied families.
Weak Measurements and Counterfactual Computation (2006)

Vaidman, in a recent article adopts the method of 'quantum weak measurements in pre- and postselected ensembles' to ascertain whether or not the chained-Zeno counterfactual computation scheme proposed by Hosten et al. is counterfactual; which has been the topic of a debate on the definition of counterfactuality. We disagree with his conclusion, which brings up some interesting aspects of quantum weak measurements and some concerns about the way they are interpreted.
Analogs of Pteroylglutamic Acid. VIII. 4-Alkoxy Derivatives (1951)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalogs of Pteroylglutamic Acid. VIII. 4-Alkoxy DerivativesBarbara Roth, James M. Smith Jr., and Martin E. HultquistCite this: J. Am. Chem. Soc. 1951, 73, 6, 2869–2871Publication Date (Print):June 1, 1951Publication History Published online1 May 2002Published inissue 1 June 1951https://pubs.acs.org/doi/10.1021/ja01150a131https://doi.org/10.1021/ja01150a131research-articleACS PublicationsRequest reuse permissionsArticle Views73Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Tumor-infiltrating B cells (2012)

B cells infiltrating into solid tumors are poorly investigated despite their described positive prognostic value. Whether this antitumor potential comes from either the antigen presentation or the antibody production capacity of B cells, or both, is unknown. Our recently published method on tumor-infiltrating B lymphocyte cloning may prove helpful in unraveling the actual relevance of these cells for tumor development and response to therapy.
Thermoluminescence Originating from the Singlet Excited State of 1,4‐Diarylcyclohexane‐1,4‐diyls: A Potentially General Strategy for the Observation of Short‐Lived Biradicals (2007)

Licht ohne sichtbare Quelle: Beim Tempern einer γ-bestrahlten glasigen Matrix, die ein 2,5-Diaryl-1,5-hexadien 1 enthält, tritt intensive Thermolumineszenz (TL) auf, die auf der Grundlage von Substituenteneffekten auf λTL und DFT-Rechnungen dem angeregten Singulettzustand des Cyclohexan-1,4-diyls 12..* zugeordnet wird (im Bild ist die Thermolumineszenz von 12..* für Ar=4-XC6H4 gezeigt; von links nach rechts: X=F, H, Me, Cl, Br).
Pharmacokinetics and pharmacodynamics of CI-992 following intravenous and oral administration to cynomolgus monkeys (1998)

The purpose of this study was to characterize CI-992 pharmacokinetics and pharmacokinetics/pharmacodynamics (PK/PD) in sodium deplete monkeys. Panels of monkeys were administered CI-992 as a 1 h intravenous infusions (0.1 and 1 mg kg-1) or as single oral doses (0, 10, 50, and 100 mg kg-1). Mean arterial blood pressure (MABP) was monitored and serial blood samples were collected up to 24 h postdose. Plasma CI-992 concentrations were quantitated by radioimmunoassay. Pharmacokinetic parameters were calculated by noncompartmental methods. PK/PD relationships were assessed by standard methods. Oral bioavailability of CI-992 in the monkeys was < 2%; steady-state volume of distribution was 0.67 L kg-1; clearance was 10.4 mL min-1 kg-1. Following oral administration, tmax generally occurred 6-9 h postadministration; plasma CI-992 concentrations increased with increasing dose between 10 and 50 mg kg-1, but did not change appreciably from 50 to 100 mg kg-1. After intravenous administration, change in MABP was correlated with plasma CI-992 concentration through an effect compartment model in which the maximum achievable effect was a 22 mm Hg decrease in MABP; the steady-state concentration which produced half the maximum effect was 11 ng mL-1. Following the 10 mg kg-1 oral dose the maximum decrease in MABP was 19.1 mm Hg; higher doses did not produce greater maximum response but increased the duration of action. In contrast to observations following intravenous administration, a trend for decreasing MABP with increasing plasma CI-992 was not apparent following oral CI-992 administration.
Tissue specific expression and cDNA structure of a human transcript encoding a nucleic acid binding [oligo(dC)] protein related to the pre-mRNA binding protein K (1994)

In human cells at least 20 different proteins or groups of proteins have been identified that are associated with hnRNAs. These proteins (designated A1-U) are highly abundant in the nucleus. In this study, we present the sequence of a novel cDNA clone, sub2.3, isolated from a human lymphocyte cDNA library. The predicted amino acid sequence shows homology to repeated domains in the human hnRNA binding protein K (hnRNP K), which are believed to be of functional importance. hnRNP K is among the major oligo(rC/dC) binding proteins in vertebrate cells and we show here that the protein product of sub2.3 also binds to oligo(dC). This is shown by a novel approach where we demonstrated specific binding of in vitro translated sub2.3 protein to biotinylated oligo(dC) which was immobilized on magnetic streptavidin-coated Dynabeads. Moreover we found that the sub2.3 transcript is expressed in a tissue dependent manner with the highest expression observed in several lymphoid tissues and skeletal muscle. The gene was also abundantly expressed in several lymphoid cell lines and the hepatoma cell line HepG2 while a low expression was observed in the HL60 myeloid cell line and in the HeLa cervical carcinoma cell line. In conclusion, this study presents the cDNA sequence of a novel transcript which shows tissue specific expression and encodes a protein with oligo(dC) binding specificity in vitro.
Catalytic properties of low-valent lanthanide species introduced into Y-zeolite (1992)

Low-valent ytterbium or europium species were introduced into Y-zeolites by impregnation from ytterbium or europium metal dissolved in liquid ammonia. The zeolites thus loaded with ytterbium or europium have a high catalytic activity for the isomerization of but-1-ene at 273 K, when they were heated under vacuum at ca. 500 K. Their catalytic activities are strongly influenced by the alkali-metal cations present in the Y-zeolite. The isomerization proceeds via an allylic carbanion-type intermediate. On the other hand, the zeolites heated under vacuum around 900 K are active for the hydrogenation of ethene at 273 K, and buta-1,3-diene at 323 K. The photoluminescence of europium supported on zeolites and temperature-programmed desorption spectra of gases desorbed from zeolites loaded with europium or ytterbium suggest that metal imides such as EuNH, and metal-like species may be formed by the zeolites under vacuum at ca. 500 and 900 K, respectively.
Gestational Diabetes and Incident Heart Failure: A Cohort Study (2021)

&lt;b&gt;Objective: &lt;/b&gt;To assess whether gestational diabetes mellitus (GDM) is associated with an increased risk of heart failure (HF). &lt;p&gt;&lt;b&gt;Research Design and Methods&lt;/b&gt;: We conducted a population-based cohort study using information from the Ministry of Health and Long Term Care of Ontario (Canada) health-care administrative databases. We identified all women in Ontario with a GDM diagnosis with a livebirth singleton delivery between July 1, 2007 and March 31, 2018. Women with diabetes or HF before pregnancy were excluded. GDM was defined based on laboratory test results and diagnosis coding. The primary outcome was incident HF hospitalization, over a period extending from the index pregnancy until March 31, 2019. The secondary outcome was prevalent peripartum cardiomyopathy (PPCM) at index pregnancy. Estimates of association were adjusted for relevant cardiometabolic risk factors.&lt;/p&gt; &lt;p&gt;&lt;b&gt;Results&lt;/b&gt;: Among 906,319 eligible women (mean age: 30 years [SD: 5.6], 50,193 with GDM [5.5%]), there were 763 HF events over a median 7 years. GDM was associated with a higher risk of incident HF (adjusted hazard ratio [aHR]: 1.62, 95% CI: 1.28, 2.05), compared to no-GDM. This association remained significant after accounting for chronic kidney disease, post-partum diabetes, hypertension, and coronary artery disease (aHR: 1.39, 95% CI: 1.09, 1.79). GDM increased the odds of PPCM (adjusted odds ratio: 1.83, 95% CI: 1.45, 2.33).&lt;/p&gt; &lt;p&gt;&lt;b&gt;Conclusions&lt;/b&gt;: In a large observational study, GDM was associated with an increased risk of HF. Consequently, diabetes screening during pregnancy is suggested to identify women at risk of HF.&lt;/p&gt; &lt;br&gt; &lt;p&gt; &lt;/p&gt;
Studies on the flower pigments in snapdragon, Antirrhinum majus L. : II. Behaviour of a certain blocking gene for the aurone synthesis (1968)

1. It was made clear that the process of aurone formation in the flower petals of garden snapdragon, Antirrhinum mujus, L, could be affected by a definite gene which was induced mutationally in an albino white flower strain maintained throughout several generations by self-polination. 2. The crossing tests and the biochemical analyses, such as paper-chromatography and spectrophotometric measurement, with the aurone pigments in flower petals of F_1 hybrids have been carried out. The F_1 hybrids used were raised from the parallel crossing between an albino white strain provided with the present aurone blocking gene and certain normal cyanic strains, as well as between the normal albino strain and the same coloured strains. 3. In the former F_1 hybrid group, the process of aurone formation in petals was completely checked by this gene, which behaved as a simple dominant one, and those hybrid forms could contribute, in consequence, to the new breeding of variously mono-coloured beautiful snapdragon varieties.
TGF-β-dependent reprogramming of amino acid metabolism induces epithelial–mesenchymal transition in non-small cell lung cancers (2021)

Epithelial-mesenchymal transition (EMT)-a fundamental process in embryogenesis and wound healing-promotes tumor metastasis and resistance to chemotherapy. While studies have identified signaling components and transcriptional factors responsible in the TGF-β-dependent EMT, whether and how intracellular metabolism is integrated with EMT remains to be fully elucidated. Here, we showed that TGF-β induces reprogramming of intracellular amino acid metabolism, which is necessary to promote EMT in non-small cell lung cancer cells. Combined metabolome and transcriptome analysis identified prolyl 4-hydroxylase α3 (P4HA3), an enzyme implicated in cancer metabolism, to be upregulated during TGF-β stimulation. Further, knockdown of P4HA3 diminished TGF-β-dependent changes in amino acids, EMT, and tumor metastasis. Conversely, manipulation of extracellular amino acids induced EMT-like responses without TGF-β stimulation. These results suggest a previously unappreciated requirement for the reprogramming of amino acid metabolism via P4HA3 for TGF-β-dependent EMT and implicate a P4HA3 inhibitor as a potential therapeutic agent for cancer.
Growth hormone therapy for the elderly: the fountain of youth proves toxic (1993)

<h3>To the Editor.</h3> —We<sup>1</sup>and others<sup>2,3</sup>have proposed the use of recombinant human growth hormone (GH) therapy for the elderly in an attempt to retard the undesirable changes in muscle mass and function, body fat, and bone mineral content associated with advancing age, and the subsequent impact these have on health care costs. Our initial enthusiasm for this idea has declined as the result of an unexpectedly high incidence of side effects of GH therapy in this population. In 12 healthy older men (63 to 76 years of age) and two women (68 and 73 years of age) who received daily GH (12.5 γg/kg per day, n=12) or placebo injections (n=2) for 4 months to assess the effects of GH therapy on body composition and muscle strength, we have experienced a notable subject attrition rate related to symptoms of carpal tunnel compression, fluid retention, and arthralgia—complications not frequently
Cryoprotective Effect of the Serine-Rich Repetitive Sequence in Silk Protein Sericin (2001)

The silk proteins, fibroin and sericin, are produced in the silk gland of Bombyx mori, and hydrophilic sericin envelops fibroin with successive sticky layers in the formation of a cocoon. To study the biological functions of sericin, we focused on the serine-rich sericin peptide consisting of 38 amino acids, which is a highly conserved and internally repetitive sequence of a sericin protein. The corresponding gene was chemically synthesized, and the PCR-amplified gene was ligated to oligomerize sericin peptide and fused at the amino terminus to a His-tagged and proteolytic cleavage sequence in an inducible expression vector. When the dimers of sericin peptides were overexpressed in Escherichia coli, the transformants showed a prominent increase in cell viability after freezing in medium. Further, the purified dimeric sericin peptide from E. coli was found to be effective in protecting lactate dehydrogenase from denaturation caused by freeze-thaw. Both of these protective effects against freezing stress in cells and proteins were also observed with sericin hydrolysate. These results indicate that this unique sericin peptide, like sericin, has a high cryoprotective activity and will be valuable as a new biomaterial for industrial use.
Wind power integration in isolated grids enabled by variable speed pumped storage hydropower plant (2008)

This paper presents aspects of control and operation of a variable speed pumped storage power plant for integration of wind power in an isolated grid. A topology based on a synchronous machine and a full scale back-to-back voltage source converter is suggested for variable speed operation of the pump-turbine. With this topology, variable speed operation can be obtained in both pumping mode and generating mode, and reactive current can be controlled independently of the active power flow and the operation of the pump turbine. By utilizing the controllability of the variable speed system, power fluctuations from wind turbines can be compensated to limit the influence on the rest of the grid. At the same time the pumped storage can be controlled to take part in the frequency control of the system and also to control grid voltage or flow of reactive power. The operation of the proposed system is illustrated by simulations based on the situation on the Faroe Islands, where controllable energy storage could allow for higher penetration of renewable energy in the power system and by that reduced dependency on power generation based on fossil fuels.
An Early Study on Intelligent Analysis of Speech under COVID-19: Severity, Sleep Quality, Fatigue, and Anxiety (2020)

The COVID-19 outbreak was announced as a global pandemic by the World Health Organisation in March 2020 and has affected a growing number of people in the past few weeks. In this context, advanced artificial intelligence techniques are brought to the fore in responding to fight against and reduce the impact of this global health crisis. In this study, we focus on developing some potential use-cases of intelligent speech analysis for COVID-19 diagnosed patients. In particular, by analysing speech recordings from these patients, we construct audio-only-based models to automatically categorise the health state of patients from four aspects, including the severity of illness, sleep quality, fatigue, and anxiety. For this purpose, two established acoustic feature sets and support vector machines are utilised. Our experiments show that an average accuracy of .69 obtained estimating the severity of illness, which is derived from the number of days in hospitalisation. We hope that this study can foster an extremely fast, low-cost, and convenient way to automatically detect the COVID-19 disease.
Fine‐needle cytology of Kaposi's sarcoma in an intramammary lymphnode: Report of one case (2012)

Kaposi's sarcoma (KS) is the most common human immunodeficiency virus (HIV) infection disease-associated malignancy. It consists of an angiosarcomatous change of the epithelial and mucous membrane-associated connective tissue not only in various sites, for example, skin, gastrointestinal system, lungs, and so on, but may also involve nonepithelial organs, such as lymphnodes. An unusual localization of KS to an intramammary lymphnode is reported here. The patient, an HIV-negative 69-year-old woman with a clinical history of rheumatoid arthritis treated with hydrocortisone, had an 8-month pathological history of biopsy-proven Kaposi sarcoma of the skin with visceral extension (stomach and duodenum). The appearance of a well-defined 23 × 20 mm(2) breast nodule during chemotherapy elicited fine-needle cytology to exclude breast carcinoma. Surgical excision confirmed the cytopathological diagnosis of Kaposis's sarcoma.
From PLANNER to CONNIVER (1972)

A higher level language derives its great power from the fact that it tends to impose structure on the problem solving behavior of the user. Besides providing a library of useful subroutines with a uniform calling sequence, the author of a higher level language imposes his theory of problem solving on the user. By choosing what primitive data structures, control structures, and operators he presents, he makes the implementation of some algorithms more difficult than others, thus discouraging some techniques and encouraging others. So, to be good, a higher level language must not only simplify the job of programming, by providing features which package programming structures commonly found in the domain for which the language was designed, it must also do its best to discourage the use of structures which lead to bad algorithms.
Expression of the Pseudomonas syringae Avirulence Protein AvrB in Plant Cells Alleviates Its Dependence on the Hypersensitive Response and Pathogenicity (Hrp) Secretion System in Eliciting Genotype-Specific Hypersensitive Cell Death (1996)

Suresh Gopalan, David W. Bauer, James R. Alfano, Amy O. Loniello, Sheng Yang He, Alan Collmer, Expression of the Pseudomonas syringae Avirulence Protein AvrB in Plant Cells Alleviates Its Dependence on the Hypersensitive Response and Pathogenicity (Hrp) Secretion System in Eliciting Genotype-Specific Hypersensitive Cell Death, The Plant Cell, Vol. 8, No. 7 (Jul., 1996), pp. 1095-1105
Beneficial Effect of a Selective Adenosine A2A Receptor Antagonist in the APPswe/PS1dE9 Mouse Model of Alzheimer’s Disease (2018)

Consumption of caffeine, a non-selective adenosine A<sub>2A</sub> receptor (A<sub>2A</sub>R) antagonist, reduces the risk of developing Alzheimer's disease (AD) and mitigates both amyloid and Tau lesions in transgenic mouse models of the disease. While short-term treatment with A<sub>2A</sub>R antagonists have been shown to alleviate cognitive deficits in mouse models of amyloidogenesis, impact of a chronic and long-term treatment on the development of amyloid burden, associated neuroinflammation and memory deficits has never been assessed. In the present study, we have evaluated the effect of a 6-month treatment of APPsw/PS1dE9 mice with the potent and selective A<sub>2A</sub>R antagonist MSX-3 from 3 to 9-10 months of age. At completion of the treatment, we found that the MSX-3 treatment prevented the development of memory deficits in APP/PS1dE9 mice, without significantly altering hippocampal and cortical gene expressions. Interestingly, MSX-3 treatment led to a significant decrease of Aβ1-42 levels in the cortex of APP/PS1dE9 animals, while Aβ1-40 increased, thereby strongly affecting the Aβ1-42/Aβ1-40 ratio. Together, these data support the idea that A<sub>2A</sub>R blockade is of therapeutic value for AD.
Carbon Cone Electrodes for Selection, Manipulation and Lysis of Single Cells (2015)

Here we present initial experiments towards an integrated platform for single cell selection, manipulation and lysis. The premise is that an array of polarized conical carbon electrodes can be dipped in a cell culture, trap cells of interest using dielectrophoresis and transport them to specific locations where they can be lyzed electrically. We aim at developing an automated tool to extract intracellular components from targeted particles over specific locations, i.e., a DNA microarray or other functionalized spots. What we contribute in this work is modeling of the electric field and its gradient around carbon cones, as well as initial cone fabrication results. To the best of our knowledge, both the fabrication of conical glassy carbon electrodes and the general concept of the proposed platform are novel. Ongoing work is on demonstrating cell trapping and lysis using these conical electrodes by only varying the magnitude and frequency of their polarizing AC signal.