gebhart/patrepeval huggingface dataset:
Effect of Oral Vitamin D, 25-Hydroxycholecalciferol (25-HCC) and Whole-Body Ultra-Violet Irradiation on Plasma 25-HCC Levels in Man (1973)

Conference Abstract| February 01 1973 Effect of Oral Vitamin D, 25-Hydroxycholecalciferol (25-HCC) and Whole-Body Ultra-Violet Irradiation on Plasma 25-HCC Levels in Man T. C. B. Stamp; T. C. B. Stamp 1Department of Human Metabolism, University College Hospital, Washington School of Medicine, U.S.A.2Department of Medicine, The Jewish Hospital of St Louis, Washington School of Medicine, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar J. M. Round; J. M. Round 1Department of Human Metabolism, University College Hospital, Washington School of Medicine, U.S.A.2Department of Medicine, The Jewish Hospital of St Louis, Washington School of Medicine, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar J. G. Haddad J. G. Haddad 1Department of Human Metabolism, University College Hospital, Washington School of Medicine, U.S.A.2Department of Medicine, The Jewish Hospital of St Louis, Washington School of Medicine, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Clin Sci Mol Med (1973) 44 (2): 3P–4P. https://doi.org/10.1042/cs044003Pc Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation T. C. B. Stamp, J. M. Round, J. G. Haddad; Effect of Oral Vitamin D, 25-Hydroxycholecalciferol (25-HCC) and Whole-Body Ultra-Violet Irradiation on Plasma 25-HCC Levels in Man. Clin Sci Mol Med 1 February 1973; 44 (2): 3P–4P. doi: https://doi.org/10.1042/cs044003Pc Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. © 1973 The Biochemical Society and the Medical Research Society1973 Article PDF first page preview Close Modal You do not currently have access to this content.
Generation of <em>Enterobacter</em> sp. YSU Auxotrophs Using Transposon Mutagenesis (2014)

Prototrophic bacteria grow on M-9 minimal salts medium supplemented with glucose (M-9 medium), which is used as a carbon and energy source. Auxotrophs can be generated using a transposome. The commercially available, Tn5-derived transposome used in this protocol consists of a linear segment of DNA containing an R6Kγ replication origin, a gene for kanamycin resistance and two mosaic sequence ends, which serve as transposase binding sites. The transposome, provided as a DNA/transposase protein complex, is introduced by electroporation into the prototrophic strain, Enterobacter sp. YSU, and randomly incorporates itself into this host's genome. Transformants are replica plated onto Luria-Bertani agar plates containing kanamycin, (LB-kan) and onto M-9 medium agar plates containing kanamycin (M-9-kan). The transformants that grow on LB-kan plates but not on M-9-kan plates are considered to be auxotrophs. Purified genomic DNA from an auxotroph is partially digested, ligated and transformed into a pir+ Escherichia coli (E. coli) strain. The R6Kγ replication origin allows the plasmid to replicate in pir+ E. coli strains, and the kanamycin resistance marker allows for plasmid selection. Each transformant possesses a new plasmid containing the transposon flanked by the interrupted chromosomal region. Sanger sequencing and the Basic Local Alignment Search Tool (BLAST) suggest a putative identity of the interrupted gene. There are three advantages to using this transposome mutagenesis strategy. First, it does not rely on the expression of a transposase gene by the host. Second, the transposome is introduced into the target host by electroporation, rather than by conjugation or by transduction and therefore is more efficient. Third, the R6Kγ replication origin makes it easy to identify the mutated gene which is partially recovered in a recombinant plasmid. This technique can be used to investigate the genes involved in other characteristics of Enterobacter sp. YSU or of a wider variety of bacterial strains.
Photoinduced Multistable Resonance Frequency Switching of Phase Change Microstring at Room Temperature (2021)

Abstract Vanadium dioxide (VO 2 ), a promising phase change material, exhibits insulator to metal transition at 68 °C, manifests a drastic change in multiple physical properties, such as electrical resistance, mechanical modulus, lattice parameters, etc. From technological perspective, the transition temperature can be reduced by precise strain engineering. Here a noncontact, all‐optical, and highly energy efficient platform is demonstrated to study macroscopic dynamics related to the localized structural rearrangements at room temperature. A thin layer (≈25 nm) of polycrystalline VO 2 deposited on a platinum coated silicon nitride microstring resonator shows a fast controlled mechanical resonance frequency response upon variations in optical power and wavelength. It is shown multiple stable frequencies of the resonator, designated as different equilibrium states, can be activated at different optical powers (≈200 µW) and wavelength, i.e., 450, 520, and 635 nm. The observed multiple resonance states of the microstring are explained because of the generation of stress due to the interplay between thermal expansion and the temperature‐induced phase change of VO 2 . It is believed this change in frequency states under the controlled external optical excitation can have potential applications in ultrafast optical switching, intelligent temperature sensors, and neuromorphic devices operated at room temperature.
A novel and efficient method to initialize FPGA embedded memory content in asymptotically constant time (2016)

This paper describes analysis and implementation of a new method for maintaining valid content of FPGA memory blocks with an asymptotically constant time synchronous clear ability, that can be useful for (re)initialization to one default value. A particular application can be for high-speed real-time LZ77 [1] lossless compression algorithms, where a dictionary has to be (re)initialized before each run of the implemented compression algorithm. The method is based on two most widely used techniques for clearing the memory content: a linear passage of the memory and clearing each cell by writing a default value and creating a register field providing an (in)valid bit for each memory cell. Our solution combines these two techniques together with the use of FPGA distributed memory blocks implemented in LUTs (Look-Up Tables) to overcome negative features of each previous method without losing the most of positive features. Our solution provides a balance between the two previous techniques and exceeds them in speed, resources utilization and latency of (re)initialization.
Work Function Characterization of the Directionally Solidified LaB6 VB2 Eutectic (2018)

With its low work function and high mechanical strength, the LaB6/VB2 eutectic system is an interesting candidate for high performance thermionic emitters. For the development of device applications, it is important to understand the origin, value, and spatial distribution of the work function in this system. Here we combine thermal emission electron microscopy and low energy electron microscopy with Auger electron spectroscopy and physical vapour deposition of the constituent elements to explore physical and chemical conditions governing the work function of these surfaces. Our results include the observation that work function is lower (and emission intensity is higher) on VB2 inclusions than on the LaB6 matrix. We also observe that the deposition of atomic monolayer doses of vanadium results in surprisingly significant lowering of the work function with values as low as 1.1 eV.
The amino acid sensor GCN2 suppresses terminal oligopyrimidine (TOP) mRNA translation via La-related protein 1 (LARP1) (2022)

La-related protein 1 (LARP1) has been identified as a key translational inhibitor of terminal oligopyrimidine (TOP) mRNAs downstream of the nutrient sensing protein kinase complex, mTORC1. LARP1 exerts this inhibitory effect on TOP mRNA translation by binding to the mRNA cap and the adjacent 5′TOP motif, resulting in the displacement of the cap-binding protein eIF4E from TOP mRNAs. However, the involvement of additional signaling pathway in regulating LARP1-mediated inhibition of TOP mRNA translation is largely unexplored. In the present study, we identify a second nutrient sensing kinase GCN2 that converges on LARP1 to control TOP mRNA translation. Using chromatin-immunoprecipitation followed by massive parallel sequencing (ChIP-seq) analysis of activating transcription factor 4 (ATF4), an effector of GCN2 in nutrient stress conditions, in WT and GCN2 KO mouse embryonic fibroblasts, we determined that LARP1 is a GCN2-dependent transcriptional target of ATF4. Moreover, we identified GCN1, a GCN2 activator, participates in a complex with LARP1 on stalled ribosomes, suggesting a role for GCN1 in LARP1-mediated translation inhibition in response to ribosome stalling. Therefore, our data suggest that the GCN2 pathway controls LARP1 activity via two mechanisms: ATF4-dependent transcriptional induction of LARP1 mRNA and GCN1-mediated recruitment of LARP1 to stalled ribosomes.
Tools for Characterizing Bacterial Protein Synthesis Inhibitors (2013)

Many antibiotics inhibit the growth of sensitive bacteria by interfering with ribosome function. However, discovery of new protein synthesis inhibitors is curbed by the lack of facile techniques capable of readily identifying antibiotic target sites and modes of action. Furthermore, the frequent rediscovery of known antibiotic scaffolds, especially in natural product extracts, is time-consuming and expensive and diverts resources that could be used toward the isolation of novel lead molecules. In order to avoid these pitfalls and improve the process of dereplication of chemically complex extracts, we designed a two-pronged approach for the characterization of inhibitors of protein synthesis (ChIPS) that is suitable for the rapid identification of the site and mode of action on the bacterial ribosome. First, we engineered antibiotic-hypersensitive Escherichia coli strains that contain only one rRNA operon. These strains are used for the rapid isolation of resistance mutants in which rRNA mutations identify the site of the antibiotic action. Second, we show that patterns of drug-induced ribosome stalling on mRNA, monitored by primer extension, can be used to elucidate the mode of antibiotic action. These analyses can be performed within a few days and provide a rapid and efficient approach for identifying the site and mode of action of translation inhibitors targeting the bacterial ribosome. Both techniques were validated using a bacterial strain whose culture extract, composed of unknown metabolites, exhibited protein synthesis inhibitory activity; we were able to rapidly detect the presence of the antibiotic chloramphenicol.
Self oscillation at millimeter-wave frequencies and modulation using optoelectronic mixing in a two-heterojunction bipolar photo-transistors configuration (2001)

We report an advanced millimeter-wave source comprising two bipolar heterojunction photo transistors: one serving as a self oscillator and the second as an optoelectronic modulator. Optical injection locking of the first as well as analog and digital modulation of the second have been demonstrated.
Notes on the biology, ecology, and use of a small Amazonian palm : LEPIDOCARYUM TESSMANNII (1987)

Lepidocaryum tessmannii Burret is a small palm which is abundant in the understory of some tropical rain forests in the Peruvian Amazonia (Fig. 1). This species is locally known as “irapay.” It is a clustering palm with slender stems, not more than 3-4 cm in diameter and 6 m in height. The leaves are palmate, like those of its giant relative, Mauritia $exuosa L.f., but they have only 4 segments inserted at the end of a 1.2 m long petiole. Each axis bears 4 to 7 contemporaneous leaves. All the species of the genus are dioecious. The “irapay” is distributed throughout Peruvian Amazonia; eastwards, it does not reach central Amazonia. This small palm is remarkable for its highly unusual growth pattern with vegetative propagation, its density in the understory of the forests surveyed, its topographic distribution which is affected by soil drainage, and its use for thatching house roofs. Our observations were made in forests near the village of Jenaro Herrera on the Ucayali River (4’55’18” Lat S; 73’40‘36” Long W), about 200 km southwest of Iquitos. The height above sea level is 125 m. The climate belongs to the tropical humid type with about 2.9 m annual rainfall and an average temperature of 26” C.
Cavitation and contrast: The use of bubbles in ultrasound imaging and therapy (2009)

Microbubbles and cavitation are playing an increasingly significant role in both diagnostic and therapeutic applications of ultrasound. Microbubble ultrasound contrast agents have been in clinical use now for more than two decades, stimulating the development of a range of new contrast-specific imaging techniques which offer substantial benefits in echocardiography, microcirculatory imaging, and more recently, quantitative and molecular imaging. In drug delivery and gene therapy, microbubbles are being investigated/developed as vehicles which can be loaded with the required therapeutic agent, traced to the target site using diagnostic ultrasound, and then destroyed with ultrasound of higher intensity energy burst to release the material locally, thus avoiding side effects associated with systemic administration, e.g. of toxic chemotherapy. It has moreover been shown that the motion of the microbubbles increases the permeability of both individual cell membranes and the endothelium, thus enhancing therapeutic uptake, and can locally increase the activity of drugs by enhancing their transport across biologically inaccessible interfaces such as blood clots or solid tumours. In high-intensity focused ultrasound (HIFU) surgery and lithotripsy, controlled cavitation is being investigated as a means of increasing the speed and efficacy of the treatment. The aim of this paper is both to describe the key features of the physical behaviour of acoustically driven bubbles which underlie their effectiveness in biomedical applications and to review the current state of the art.
Superconducting current transfer devices for use with a superconducting LVdc mesh (1994)

This paper describes engineering analysis and simulation results for a device intended to transfer current among parallel superconducting branches in a meshed superconducting dc system. Meshed superconducting dc systems offer unique problems of current distribution between parallel paths due to the lack of resistance. Neither resistances nor steady-state impedances can be relied upon to equalize the loading among superconducting branches, or to prevent any specific branch from exceeding its critical current and quenching. The paper describes the design and operation of a superconducting current diverter based on the creation of a normal zone in a specially designed superconducting cable section. The current diverter can operate successfully with a surprisingly small normal state resistance. The main disadvantage of these devices is the loss of energy associated with the resistance of the device when activated. These devices appear promising for both routine line operations and for current steering in meshed superconducting dc systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cartellisation ou recomposition ? Le SPD et la CDU/CSU allemands face aux nouveaux partis (2021)

Selon Peter Mair et de Richard Katz, les partis politiques dominants se caracterisent par un phenomene de cartellisation. Ils tendraient a cooperer ensemble pour exclure les nouveaux concurrents et pour monopoliser les ressources de l’Etat. Nous questionnons cette hypothese a l’exemple de l’evolution du systeme politique allemand entre 2005 et 2020. Nous constatons un declin constant des milieux sociaux traditionnels soutenant la CDU/CSU et le SPD. Le nombre de chretiens, d’agriculteurs d’une part et de salaries syndiques d’autre part – soutien respectif des democrates-chretiens et des sociaux-democrates – baisse de maniere tendancielle. Neanmoins, les partis etablis n’ont d’acces privilegie aux ressources de l’Etat lesquelles sont accessibles aussi aux nouveaux partis politiques – Alternative fur Deutschland, PDS/Die Linke, Verts. On constate, en revanche, une autre forme de cartellisation que nous appelons cartellisation-recomposition. Elle repose sur un rapprochement entre les partis etablis entre eux et avec certains nouveaux partis. La cartellisation-exclusion concerne uniquement certains partis et elle s’inscrit dans une logique de « cordon sanitaire » et non de monopolisation des ressources publiques.
La transmission de PME : de l'influence du comportement des acteurs et du transfert de l'organisation (2006)

Nous defendons la these de l'influence determinante des comportements des Cedants et des Repreneurs sur l'issue d'une transmission, qui peut etre interpretee comme la gestion du transfert de son organisation. Nous analysons les motifs d'echec et de reussite d'une transmission, et nous montrons la faible influence des situations initiales economiques et organisationnelles sur l'issue. Nous reconnaissons les comportements et les combinaisons favorables a la reussite et nous identifions les caracteristiques et les motivations des acteurs determinant leurs comportements. Nous etudions la transmission de l'organisation a travers I'analyse : du transfert des actifs intangibles, des liens formalises, des savoirs et des competences, et du recours au processus de transmission. Nous interpretons la transmission comme un processus de transfert de son organisation. Nous identifions les strategies de transmission et nous proposons une modelisation du pilotage du processus que nous testons lors de 2 recherches-interventions. Enfin nous montrons les apports theoriques et pratiques de ces travaux, et nous proposons pistes de prolongement de recherche.
The Flexible Pointer: An Interaction Technique for Selection in Augmented and Virtual Reality (2003)

We present a virtual flexible pointer that allows a user in a 3D environment to point more easily to fully or partially obscured objects, and to indicate objects to other users more clearly. The flexible pointer can also reduce the need for disambiguation and can make it possible for the user to point to more objects than currently possible with existing egocentric techniques.
Cavity length detuning characteristics of the synchronously mode-locked CW dye laser (1979)

We present both theoretical and experimental details on the effects of cavity length deturning of the synchronously mode-locked CW dye laser. The theory predicts an increase in cavity length sensitivity with intracavity bandwidth, and an asymmetric variation in the pulsewidth and pulse power with length detuning from the "matched" position. The predictions are shown to be in close agreement with the results of our experiments on the Rhodamine 6G dye laser.
Chest Tube–Related Empyema Due to Methicillin-ResistantStaphylococcus aureus:Could the Chest Tube Be Coated With Antiseptics? (2006)

We reviewed the epidemiology, clinical manifestations, and outcomes of 3 cases of chest tube-related empyema due to methicillin-resistant Staphylococcus aureus (MRSA). Antiseptic-impregnated chest tubes were inserted in cultures containing MRSA isolates from these 3 patients, and zone of inhibition were measured. Chest tube-related MRSA empyema might complicate tube thoracostomy, and coating the chest tube with antiseptic agents could prevent this complication.
His bundle pacing insights from electroanatomical mapping: Topography and pacing targets (2020)

(1) Pacemapping identifies distinct regions that may correspond to HB anatomy. (2) A linear S-HBP area is typically surrounded by two separate NS areas. (3) Pace-map ECGs from upper and lower NS-HBP areas have different morphologies. (4) These EAM features and pace-mapping may be helpful to the implanter.
An Enantiospecific Synthesis of Jiadifenolide (2014)

A Robinson annulation, van Leusen homologation, and a desymmetrizing C-H oxidation enabled an enantiospecific synthesis of the neurotrophic natural product jiadifenolide. From a pulegone-derived building block, a key propellane intermediate was constructed through the use of simple reagents in a highly diastereoselective fashion. A short series of oxidations of this tricylic framework allowed progression to the natural product.
Where Do We Go From Here: Memphis and the Legacy of Dr. King’s Unfinished Work (2019)

Following the 50th anniversary of Dr. Martin Luther King Jr.'s murder, this essay examines King's unfinished work in the areas of education, voting, criminal justice, and public health in the place of King's assassination, Memphis, Tennessee. Authors also examine the current work of contemporary Memphis activists in light of King's legacy.
p16INK4 Expression Is Associated with the Increased Sensitivity of Human Non‐small Cell Lung Cancer Cells to DNA Topoisomerase I Inhibitors (1997)

Inactivation of p16INK4, an inhibitor of cyclin-dependent kinases 4 (CDK4) and 6 (CDK6), may be essential for oncogenesis in non-small cell lung cancer (NSCLC). We examined the sensitivity of two clones of p16INK4-transfected NSCLC cell line with homozygous deletion of p16INK4, A549/p16-1 and 2, to DNA topoisomerase I (topo I) inhibitors. A549/p16-1 and -2 showed 7.7- and 9.1-fold increases in sensitivity to CPT-11 (11,7-ethyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxycamptothecin ), respectively, compared with A549 cells. Ectopic p16INK4-expressing cells also showed approximately 4.0-fold increase in sensitivity to SN-38 (7-ethyl-10-hydroxycamptothecin), the active metabolite of CPT-11, compared to the parent cells. The topo I-mediated DNA relaxation activities of ectopic p16INK4-expressing cells were approximately 5 times higher than those of the parent cells. Northern and western blot analyses indicate that these increased topo I activities of ectopic p16INK4-expressing cells were due to an elevated topo I mRNA level and an increase in topo I protein. The chemosensitivity to topo I inhibitors, topo I mRNA level, protein content and activity of a p16INK4 revertant, lacking functional p16INK4, tended to be restored toward those of the parental phenotype to some extent. These results suggest that p16INK4 expression is closely associated with the increased sensitivity of ectopic p16INK4-expressing NSCLC cells to topo I inhibitors. The up-regulation of topo I mRNA level, protein content and activity may be responsible for this hypersensitivity.