gebhart/patrepeval huggingface dataset:
Massive Immune Hemolysis After Non-myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation with Minor ABO-incompatibility (2002)

AbstractA 35-year-old male, blood group B, Rh(D)+ type, received an allogeneic peripheral blood stem cell (PBSC) transplant after a non-myeloablative regimen of fludarabine and cyclophosphamide for resistant γ δ cutaneous T-cell lymphoma (CTCL). The donor was his HLA-identical brother, blood group O, Rh(D)+ type. Graft-versus-host disease (GVHD) prophylaxis was performed with cyclosporine alone. On day +8, massive immune hemolysis occurred, followed by acute renal failure. Hemodialysis was performed eight times until recovery of renal function on day +24. The risk of delayed immune hemolysis after non-myeloablative allogeneic PBSC transplantation with minor ABO-incompatibility must be considered.KeywordsAllogeneic PbsctMinor Abo-incompatibilityImmune HemolysisRed Cell Antigen
High-performance type-II InAs/GaSb superlattice photodiodes with cutoff wavelength around 7 μm (2005)

We report the most recent result in the area of type-II InAs/GaSb superlattice photodiodes that have a cutoff wavelength around 7 μm at 77 K. Superlattice with a period of 40 Å lattice matched to GaSb was realized using GaxIn1−x type interface engineering technique. Compared with significantly longer period superlattices, we have reduced the dark current density under reverse bias dramatically. For a 3 μm thick structure, using sulfide-based passivation, the dark current density reached 2.6×10−5A∕cm2 at −3 V reverse bias at 77 K. At this temperature the photodiodes have R0A of 9300Ωcm2 and a thermally limited zero bias detectivity of 1×1012cmHz1∕2∕W. The 90%–10% cutoff energy width was only 16.5 meV. The devices did not show significant dark current change at 77 K after three months storage in the atmosphere.
TRPV4‐associated alterations of Ca 2+ pulsars in a murine model of pulmonary hypertension secondary to heart failure (1090.11) (2014)

Heart failure is the most frequent cause of pulmonary hypertension (PH) (group 2). Pathophysiological mechanisms modulating pulmonary vascular tone and leading to group 2‐PH are poorly defined. Since Ca 2+ homeostasis is crucial to endothelial control of vascular tone, mechanisms involved, including TRPV4 channels, may be altered. This study aimed to characterize intracellular Ca 2+ dynamics in pulmonary endothelium and their alterations in group 2 PH. A mouse model of group 2 PH was developed and 3 rd order resistance arteries were isolated to investigate endothelial intracellular Ca 2+ . TRPV4 involvement was studied using a high‐speed confocal microscope. Characterization of endothelial localized calcium transients, similar to Ca 2+ pulsars, was carried out in pulmonary arteries from control mice. Exposure of Sham arteries to GSK1016790A (100 nM), a TRPV4‐agonist, increased the frequency (1.5 fold) of Ca 2+ pulsars. However, exposure to GSK1016790A had little if any effect on Ca 2+ dynamics in PH arteries. TRPV4 channel expression at both mRNA and protein levels within pulmonary endothelium was confirmed by qPCR, IHC and WB approaches. Our data suggest a potential role for endothelial TRPV4 in vasoregulatory alterations involving local Ca 2+ dynamics. This study strengthens our understanding of endothelial Ca 2+ dyshomeostasis occurring in a clinically relevant model of PH. Grant Funding Source : FRQS, HSFC, FICM, CIHR
Actividad física y deporte en jóvenes en riesgo: educación en valores (2005)

En este articulo hacemos una revision y presentamos los principales estudios y lineas de trabajo que se vienen realizando para educar en valores a jovenes en riesgo a traves de la actividad fisica y el deporte. Hacemos un recorrido a traves de las caracteristicas que definen a este tipo de poblaciones, los aspectos mas importantes a trabajar en valores, los errores mas comunes que se han cometido en los programas de intervencion, y la necesidad de trabajar desde su propia cultura y partir de la nocion de modelos ecologicos integrados.
Hydrogen/Deuterium Exchange- and Protease Digestion-Based Screening Assay for Protein−Ligand Binding Detection (2009)

A protease digestion strategy was incorporated into single-point stability of unpurified proteins from rates of H/D exchange (SUPREX), which is a hydrogen/deuterium (H/D) exchange- and mass spectrometry-based assay for the detection of protein-ligand binding. Single-point SUPREX is an abbreviated form of SUPREX in which protein-ligand binding interactions are detected by measuring the increase in a protein's thermodynamic stability upon ligand binding. The new protease digestion protocol provides a noteworthy increase in the efficiency of single-point SUPREX because peptide masses can be determined with greater precision than intact protein masses in the matrix-assisted laser desorption ionization (MALDI) readout of single-point SUPREX. The protocol was evaluated in test screens on two model protein systems, including cyclophilin A (CypA) and the minor allele variant of human alanine:glyoxylate aminotransferase (AGTmi). The test screening results obtained on both proteins revealed that the peptide readout of the single-point SUPREX-protease digestion protocol was more efficient than the intact protein readout of the original single-point SUPREX protocol at discriminating hits and nonhits. In addition to this improvement in screening efficiency, the protease digestion strategy described here is expected to significantly increase the generality of the single-point SUPREX assay.
Perceptions concerning hip resurfacing from attendees at the Second Annual U.S. Comprehensive Course on Total Hip Resurfacing Arthroplasty. (2009)

Resurfacing hip arthroplasty represents a new, alternative technology to standard total hip arthroplasty. We examined the perceptions about resurfacing after the Second Annual United States Comprehensive Course on Total Hip Resurfacing Arthroplasty, which was a multi-company sponsored educational event to advance general knowledge, basic science, and surgical skills relevant to this field. The course led to a substantial increase in knowledge concerning resurfacing as evaluated from pre- and post-tests. The perceptions concerning indications, patient selection, and beliefs of the course attendees are reported.
Method for testing earth samples for contamination by organic contaminants (2023)

Provided is a method for testing earth samples for contamination by organic contaminants, and particularly for aromatic compounds such as those found in diesel fuel and other heavy fuel oils, kerosene, creosote, coal oil, tars and asphalts. A drying step is provided in which a drying agent is contacted with either the earth sample or a liquid extract phase to reduce to possibility of false indications of contamination that could occur when humic material is present in the earth sample. This is particularly a problem when using relatively safe, non-toxic and inexpensive polar solvents such as isopropyl alcohol since the humic material tends to be very soluble in those solvents when water is present. Also provided is an ultraviolet spectroscopic measuring technique for obtaining an indication as to whether a liquid extract phase contains aromatic organic contaminants. In one embodiment, the liquid extract phase is subjected to a narrow and discrete band of radiation including a desired wave length and the ability of the liquid extract phase to absorb that wavelength of ultraviolet radiation is measured to provide an indication of the presence of aromatic organic contaminants.
CloudSat spaceborne 94 GHz radar bright bands in the melting layer: An attenuation‐driven upside‐down lidar analog (2007)

The CloudSat satellite supports a W‐band (94 GHz) cloud profiling radar. At this 3.2 mm wavelength, ground‐based measurements of rainfall associated with melting snowflakes do not show the radar reflectivity peak that is characteristic of bright band measurements at longer (Rayleigh scattering‐dominated) wavelengths. Nonetheless, examination of downward‐looking CloudSat returns in precipitation often indicate an obvious signal peak in the melting region. Through melting layer microphysical and scattering model simulations, we demonstrate that this downward‐viewing radar feature is analogous to the lidar bright band observed from the ground in that it owes its existence to strong attenuation. In the upward‐looking lidar case, the strong attenuation comes from large low‐density snowflakes. In the downward‐looking 94 GHz radar case, it is due to the effects of the greater refractive index of water particles compared to ice: it is comparable to an upside‐down lidar bright band. A W‐band radar dark band, which contributes to the visibility of the bright band, is shown to be due to attenuation in the snowfall. For comparison, the bright and dark bands for an upward viewing lidar are also modeled: the latter is simulated by a reduction in light backscattering efficiency of ice‐containing raindrops.
Thickness of a liquid film on a solid substrate induced by steady-state surfactant flow (1989)

When a wettable solid substrate (flint glass) is brought into contact with a vessel of water on the surface of which an appropriate surfactant has been spread (valinomycin), a visible film is observed to creep up to the substrate. If the substrate is now bent so that its far end makes contact with a second water vessel (at the same height) on which the same surfactant is spread, a net flow of surfactant is observed to occur under the influence of a surface tension differential between the two vessels. From elementary hydrodynamics the thickness of the film of water accompanying the surfactant flow can be deduced from measurements of the surface tension differential and the rate of transfer of the surfactant. We have developed a theory for the thickness of this entrained film, which turns out is governed by the van der Waals dispersion force. Our treatment allows us to deduce the film thickness from first principles and, by comparison with the experimental results, to determine the retarded Hamaker constant B.
Production kinetics of acidophilin 801, a bacteriocin produced by Lactobacillus acidophilus IBB 801 (2000)

Lactobacillus acidophilus IBB 801 produces a small bacteriocin, designated acidophilin 801. Studying the relationship between growth and bacteriocin biosynthesis revealed primary metabolite kinetics of bacteriocin production with a peak activity at the end of the exponential growth phase followed by a decrease during the stationary phase. Both microbial growth and bacteriocin production was inhibited by lactic acid. Whereas volumetric bacteriocin production (activity units (AU) ml−1) was favoured under pH-controlled conditions, bacteriocin titres rapidly decreased because of strong adsorption of the bacteriocin molecules to the producing cells under less acidic conditions.
Lung Injury Repair by Transplantation of Adult Lung Cells Following Preconditioning of Recipient Mice (2017)

Repair of injured lungs represents a longstanding therapeutic challenge. We recently demonstrated that human and mouse embryonic lung tissue from the canalicular stage of development are enriched with lung progenitors, and that a single cell suspension of canalicular lungs can be used for transplantation, provided that lung progenitor niches in the recipient mice are vacated by strategies similar to those used in bone marrow transplantation. Considering the ethical limitations associated with the use of fetal cells, we investigated here whether adult lungs could offer an alternative source of lung progenitors for transplantation. We show that intravenous infusion of a single cell suspension of adult mouse lungs from GFP+ donors, following conditioning of recipient mice with naphthalene and subsequent sublethal irradiation, led to marked colonization of the recipient lungs, at 6-8 weeks post-transplant, with donor derived structures including epithelial, endothelial, and mesenchymal cells. Epithelial cells within these donor-derived colonies expressed markers of functionally distinct lung cell types, and lung function, which is significantly compromised in mice treated with naphthalene and radiation, was found to be corrected following transplantation. Dose response analysis suggests that the frequency of patch forming cells in adult lungs was about threefold lower compared to that found in E16 fetal lungs. However, as adult lungs are much larger, the total number of patch forming cells that can be collected from this source is significantly greater. Our study provides proof of concept for lung regeneration by adult lung cells after preconditioning to vacate the pulmonary niche. Stem Cells Translational Medicine 2018;7:68-77.
Migration processes (1996)

Optimization processes based on "active models" play central roles in many areas of computational vision as well as computational geometry. However, current models usually require highly complex and sophisticated mathematical machinery and at the same time they also suffer from a number of limitations which impose restrictions on their applicability. In this paper a simple class of discrete active models, called migration processes, is presented. The processes are based on iterated averaging over neighborhoods defined by constant geodesic distance. It is demonstrated that the migration process model combines a number of advantages of different active models. The processes can be applied to derive natural solutions to a variety of optimization problems which include: defining (minimal) surface patches given their boundary curves; finding shortest paths joining set of points; and decomposing objects into "primitive" parts.
Organ Cultures for Retinal Diseases (2020)

The successful development of novel therapies is closely linked with understanding the underlying pathomechanisms of a disease. To do so, model systems that reflect human diseases and allow for the evaluation of new therapeutic approaches are needed. Yet, preclinical animal studies often have limited success in predicting human physiology, pathology, and therapeutic responses. Moreover, animal testing is facing increasing ethical and bureaucratic hurdles, while human cell cultures are limited in their ability to represent <i>in vivo</i> situations due to the lack of the tissue microenvironment, which may alter cellular responses. To overcome these struggles, organ cultures, especially those of complex organs such as the retina, can be used to study physiological reactions to substances or stressors. Human and animal organ cultures are now well established and recognized. This mini-review discusses how retinal organ cultures can be used to preserve tissue architecture more realistically and therefore better represent disease-related changes. It also shows how molecular biological, biochemical, and histological techniques can be combined to investigate how anatomical localization may alter cellular responses. Examples for the use of retinal organ cultures, including models to study age-related macular degeneration (AMD), <i>retinitis pigmentosa</i> (RP), central artery occlusion (CRAO), and glaucoma are presented, and their advantages and disadvantages are discussed. We conclude that organ cultures significantly improve our understanding of complex retinal diseases and may advance treatment testing without the need for animal testing.
Low risk for diabetic complications in type 1 diabetes patients carrying a protective insulin gene variant (2023)

Type 1 diabetes patients carrying a 'protective' insulin gene (INS) variant present a disease endotype with reduced insulin antibody titers, preserved beta cell function and improved glycemic control. We tested whether this protective INS variant associated with lowered risk for development of proliferative diabetic retinopathy (PDR) and diabetic kidney disease (DKD) as long-term diabetic complications. Insulin gene polymorphisms were evaluated in 1,363 type 1 diabetes patients participating in the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications (DCCT/EDIC) study that compared intensive versus conventional insulin therapy in relation with development of PDR and DKD with a follow-up of over two decades. PDR and DKD were absent in type 1 diabetes patients carrying the protective INS variant and receiving intensive insulin therapy (the current standard of clinical care) 1-5 years from their diagnosis (n = 67; mean post-diagnosis follow up of 20.4 ± 1.6 years), versus 11 of 258 patients (4.3%) lacking this variant (20.4 ± 1.8 years follow up). In the secondary intervention group of the intensive therapy arm (1-15 years of disease), PDR was significantly less frequent in carriers of the protective INS variant than those without it (4 of 83 [4.8%] vs. 31 of 260 [11.9%]; p = 0.032; 26.1 ± 3.9 and 26.3 ± 4.1 years follow-up, respectively), whereas DKD frequencies were no different between those with or without this variant (5 of 83 [6.0%] vs. 11 of 260 [4.2%]). Carrying a copy of this protective INS variant further reduces the risk of diabetic complications achieved by intensive insulin therapy and marks a disease endotype with superior glycemic control, increased and extended beta cell function, and prevention of DKD and PDR.
Application of artificial neural network to computer-aided diagnosis of coronary artery disease in myocardial SPECT bull's-eye images. (1992)

We have developed a computerized system that can aid in the radiologist's diagnosis in the detection and classification of coronary artery diseases. The technique employs a neural network to analyze 201Tl myocardial SPECT bull's-eye images. This multi-layer feed-forward neural network with a backpropagation algorithm has 256 input units (pattern: compressed 16 x 16-matrix images), 5-140 units in a single hidden layer, and eight output units (diagnosis: one normal and seven different types of abnormalities). The neural network was taught using pairs of training (learning) input data (bull's-eye "EXTENT" image) and desired output data ("correct" diagnosis). The effects of the numbers of hidden units and learning iterations in the network on the recognition performance were examined. In our initial stage, the results show that the recognition performance of the neural network is better than that of the radiology resident but worse than that of the experienced radiologist. Our study also demonstrates that the result produced in the neural network depends on the variety of the training examples used. The preliminary study suggests that the neural network approach is useful for the computer-aided diagnosis of coronary artery diseases in myocardial SPECT bull's-eye images.
Helminthic Infections (2010)

Helminthic parasites are multicellular worms that possess differentiated organ systems. They (with a few exceptions) do not replicate in the human host, and they tend to elicit eosinophilia within the tissues and blood of infected humans. Helminthic parasites include nematodes (roundworms), cestodes (tapeworms), and trematodes (flukes). The major intestinal nematodes are roundworm, pinworm, hookworm, whipworm, and Strongyloides stercoralis. Trichinellosis is caused by five species of the nematode Trichinella and develops after ingestion of infected meat, usually pork or the meat of certain carnivores. Nematode infections of the major tissue are anisakiasis, visceral larva migrans,Angiostrongylus cantonensis infection, mammomonogamosis (syngamosis), gnathostomiasis, dracunculiasis, and filariasis. Trematode and cestode infections are also described in this chapter, including infections by fish, beef, and pork tapeworms, as well as cysticercosis. Disease from Paragonimus, Clonorchis, Fasciola, Fasciolopsis, and Schistosoma is covered. Echinococcus infection and hydatid cyst disease are discussed. Tables describe intestinal nematode infection and treatment and filarial parasites of humans. Figures illustrate a variety of helminthic parasites and their life cycles. This review contains 136 references.
Fabrication and characteristic evaluation of sub-micron gap liquid chromatography micro chip (2008)

We proposed a fabrication method of a sub-micron gap liquid chromatography micro chip by Silicon micromachining technology and performed a principle confirmation using this chip. This paper shows the fabrication process of the sub-micron gap in the flow channel which is made by the micro-pillar array formation by Silicon micromachining technology, and the deposition of poly-Si and successive thermal oxidation. It has become possible to fabricate the sub-micron order gap and control the gaps by the proposed method. Next, we deposited the ODS (octadecylsily) in the flow channel. Finally, using this chip, we performed the principle confirmation experiment of ODS deposition effect by injecting lysozyme into the flow channel. As the result, it was confirmed that the proposed method has the possibility of the separation analysis of biological samples.
Oral potentially malignant disorders: advice on management in primary care (2022)

Introduction: The diagnosis of and risks associated with oral potentially malignant disorders (OPMD) have been widely reported, but little has been published on the management of OPMDs in a primary dental care setting. Hospital services face ongoing pressures due to long-term follow-up, with a need for surveillance to be jointly undertaken with primary dental care clinicians. In a primary care setting, identification and surveillance of OPMDs can be challenging as no universal guidance exists on recommended recall intervals. Corpus: In this article, an update on OPMDs is provided and, based on the practices of six Oral Medicine units in Europe (London (United Kingdom), Milan (Italy), Bordeaux (France), Porto (Portugal), Zagreb (Croatia) and Santiago de Compostela (Spain)), aiming to provide guidance on monitoring in a primary care setting in Europe. Conclusion: Oral medicine clinicians can provide guidance to general dental practitioners (GDPs) on recommended recall intervals. It is important that they feel confident in monitoring these conditions and, when concerned, to arrange referral to a hospital or appropriate specialist. GDPs should document descriptions of lesions and, if possible, take clinical photographs. Patients should be counselled on modifiable lifestyle factors and directed to oral medicine society websites to access patient information leaflets.
ORGANIC POLLUTANTS IN THE COASTAL ENVIRONMENT OFF SAN DIEGO, CALIFORNIA. 2. PETROGENIC AND BIOGENIC SOURCES OF ALIPHATIC HYDROCARBONS (1997)

The results from the measurements of aliphatic hydrocarbons suggest that hydrocarbons in the Point Loma Wastewater Treatment Plant (PLWTP) effluents are mainly petroleum derived; those in the Tijuana River runoff have largely originated from terrestrial plants with visible petroleum contamination; and those in the sea surface microlayer, sediment traps, and sediments at various coastal locations off San Diego have mostly resulted from biogenic contributions with enhanced microbial products in the summer season. Rainfall in the winter season appeared to amplify the inputs from terrestrial higher plants to the coastal areas. The PLWTP discharged approximately 3.85 metric tons of n-alkanes (C10-C35) in 1994, well below the level (136 metric tons) estimated in 1979. The input of aliphatic hydrocarbons from the Tijuana River was about 0.101 metric tons in 1994. Diffusion, solubilization, evaporation, and microbial degradation seemed partially responsible for the difference in the concentrations and compositions of aliphatic hydrocarbons in different sample media, although the relative importance of each mechanism cannot be readily discerned from the available data. The results from analyses of aliphatic hydrocarbon compositional indices are generally consistent with those of polycyclic aromatic hydrocarbons.