gebhart/patrepeval huggingface dataset:
Abstract 5451: Deficiency of Glutathione Peroxidase-3 Promotes Platelet Activation in vivo (2008)

Plasma glutathione peroxidase (GPx-3) is a selenocysteine-containing protein with antioxidant properties. GPx-3 plays an important role in plasma against oxidant stress by scavenging reactive oxygen species. A deficiency of this enzyme has been associated with platelet dependent thrombosis. We recently developed an animal model to assess platelet function in GPx-3 deficient mice. We hypothesized that GPx-3 deficiency induces platelet activation in vivo . GPx-3 (−/−) mice showed an attenuated bleeding time compared with wild-type mice (94.5 ± 28.8 s versus 153.4 ± 32.3, P&lt;0.05). We also noted an increase in the plasma levels of soluble P-selectin, a marker of platelet activation and prothrombotic activity, in GPx-3 (−/−) mice compared with wild-type mice (137.8 ± 12.3 ng/ml plasma versus 101.5 ± 8.8, P&lt;0.05). Cyclic GMP, a key intracellular second messenger molecule and marker for activation of soluble guanylyl cyclase by nitric oxide, was decreased in the plasma of GPx-3 (−/−) mice compared with wild-type mice (5.38 ± 1.75 pmol/ml plasma versus 23.67 ± 3.59, P&lt;0.001), consistent with less bioactive NO in GPx-3 (−/−) mice. ADP was infused into the right ventricle of mice to induce platelet aggregation in the pulmonary vasculature; this assay resulted in higher pulmonary artery pressure in GPx-3 (−/−) compared with wild-type mice suggesting a more robust platelet activation response in the GPx-3 (−/−) mice. To confirm this interpretation, histological sections from the pulmonary vasculature of GPx-3 (−/−) compared with wild-type mice showed increased thrombi per 7.5 mm 2 section normalized to wild-type mice based on staining intensity for P-selectin (1.7 ± 0.4 versus 1.0 ± 0.1, P&lt;0.001), as well as a higher percentage of occluded vessels (0.82 ± 0.16 % versus 0.54 ± 0.21, P&lt;0.05). These findings demonstrate that GPx-3 deficiency causes platelet activation resulting in a prothrombotic state. These data illustrate the importance of this plasma antioxidant enzyme in regulating platelet activity and platelet-dependent thrombosis.
Pseudobactins bounded iron nanoparticles for control of an antibiotic‐resistant Pseudomonas aeruginosa ryn32 (2019)

Among 50 strains of Pseudomonas aeruginosa tested for the resistance to antibiotics, strain ryn32 was selected for this study based on its resistance level. It showed complete resistance toward aztreonam and almost complete resistance (96%) against kanamycin. Iron nanoparticles (FeNPs) were then prepared and found with diameters 30-50 nm. The threshold level of FeNPs for pyoverdines (PVDs) production by P. aeruginosa ryn32 was found at 25 μM concentration. PVDs production was optimal with pH 7.5, 35°C, succinate as carbon source, ammonium sulfate as nitrogen source at 60 hr fermentation time. Interestingly, when used the PVDs as conjugates with FeNPs they showed antibacterial action against the producing strain and some other gram-negative bacteria. This suggests that the conjugates enter the bacterial cell via the ferriPVDs uptake pathway, which triggers the accumulation of FeNPs inside the cell, which is crucial on bacterial viability. Growth stimulation with the same concentrations of FeNPs and PVDs in separate treatments supported this view.
Minimally Invasive Transforaminal Lumbar Interbody Fusion (TLIF) (2005)

Forty-nine patients underwent minimally invasive transforaminal lumbar interbody fusion (TLIF) from October 2001 to August 2002 (minimum 18-month follow-up). The diagnosis was degenerative disc disease with herniated nucleus pulposus (HNP) in 26, spondylolisthesis in 22, and a Chance-type seatbelt fracture in 1. The majority of cases (n = 45) were at L4-L5 or L5-S1. A paramedian, muscle-sparing approach was performed through a tubular retractor docked unilaterally on the facet joint. A total facetectomy was then conducted, exposing the disc space. Discectomy and endplate preparation were completed through the tube using customized surgical instruments. Structural support was achieved with allograft bone or interbody cages. Bone grafting was done with local autologous or allograft bone, augmented with recombinant human bone morphogenetic protein-2 in some cases. Bilateral percutaneous pedicle screw-rod placement was accomplished with the Sextant system. There were no conversions to open surgery. Operative time averaged 240 minutes. Estimated blood loss averaged 140 mL. Mean length of hospital stay was 1.9 days. All patients presenting with preoperative radiculopathy (n = 45) had resolution of symptoms postoperatively. Complications included two instances of screw malposition requiring screw repositioning and two cases of new radiculopathy postoperatively (one from graft dislodgement, the other from contralateral neuroforaminal stenosis). Narcotic use was discontinued 2-4 weeks postoperatively. Improvements in average Visual Analogue Pain Scale and Oswestry Disability Index (preoperative to last follow-up) scores were 7.2-2.1 and 46-14, respectively. At last follow-up, all patients had solid fusions by radiographic criteria. Results of this study indicate that minimally invasive TLIF is feasible and offers several potential advantages over traditional open techniques.
Economic Impact of Proximal Junctional Kyphosis (2020)

Proximal junctional kyphosis (PJK) is a common occurrence after posterior thoracolumbar instrumented spinal fusions for adult spinal deformity (ASD). Despite its relative frequency, PJK’s economic impact has only recently gained appreciation and attention. On the basis of the available literature, direct costs associated with revision surgery for PJK range from ∼$20,000 to $120,000. The average direct costs for revision operations for PJK in the lower thoracic spine are similar to the costs for revision operations for PJK in the upper thoracic spine. Variability in costs may be attributable to variations in surgical strategies and individual hospital’s postoperative care protocols and pricing. In the discussion to follow, the economic impact of PJK after operative intervention for ASD will be showcased by presenting it in the larger context of our health care system’s focus on value-based care, the health burden of ASD, and quality and costs associated with ASD operations.
Analytical Evaluation of Tri-band Printed Antenna (2012)

Accepted: blished online: Abstract: Simulated design and analysis of a simple tri-band microstrip antenna is presented in this paper. A thin transmission line is used to excite the rectangular patch and FR4 epoxy with relative permittivity of 4.4 is used as substrate. To confirm the proficiency of proposed antenna, simulations have been performed in Ansoft HFSS (High Frequency Structure Simulator) a 3D electromagnetic field simulator, based on finite element method. Impedance bandwidths are determined on -10 dB at resonating frequencies of 2.4, 3.3 and 5.3 GHz. An introduced design covers the requisite bandwidth for IEEE Wireless Local Area Network (WLAN) standards: 802.11 a/n, b/g (Wi-Fi), Bluetooth, ZigBee, and 802.16e (WiMAX). Multiband functionality, small size, light weightiness, plain configuration and conformability are the advantages; making the proposed design a good contender for integration in handheld wireless devices.
Nutritional composition of fruit of 10 date palm (Phoenix dactyliferaL.) cultivars grown in Saudi Arabia (2014)

The aim of the study was to determine the chemical composition of dates from 10 types of palm cultivated in Saudi Arabia (Phoenix dactylifera L.) to assess their nutritive (crude protein, crude fat and ash) and amino acid composition. The dates were rich in sugar (71.2–81.4% dry weight), while ash represented 1.68–3.94%; they contained low concentrations of protein and lipid (1.72–4.73% and 0.12–0.72%, respectively). The predominant mineral was potassium, and the main sugars were glucose and fructose. They contained high concentrations of aspartic acid, proline, alanine, glycine, valine and leucine; low concentrations of threonine, serine, isoleucine, tyrosine, arginine, phenylalanine and lysine and very low concentrations of methionine and histidine. These results show that dates are nutritious and can play a major role in human nutrition and health.
Steelworks residues and the Waelz kiln treatment of electric arc furnace dust (1996)

Electric arc furnace dust with a combined zinc and lead content in excess of 20% renders the dumping of this material impossible in many countries, for both statutory and financial reasons. In the Waelz process, dust is treated in a rotary kiln where it is heated to approximately 1,200 C. lead and zinc are volatilized under reducing conditions and collected as fine dust in the off-gas dust collection system. The oxide recovered in the off-gas filters contains approximately 55% Zn and up to 10% Pb, and is ideal feedstock for the Imperial Smelting furnace for lead/zinc recovery. The remaining slag is inert and unleachable so that it can be used as a building aggregate.
Visual sensation during phacoemulsification and intraocular lens implantation using topical and regional anesthesia (2004)

Patients having phacoemulsification and PC IOL implantation under topical or regional anesthesia had a wide spectrum of visual sensations that were similar at most stages of surgery. Patients in the topical anesthesia group perceived more colors at the early stages and more blue throughout surgery than patients in the regional anesthesia group.
Microbial decolorization and bioremediation of melanoidin containing molasses spent wash. (2007)

Molasses spent wash from cane-molasses based distilleries contains a brown coloured recalcitrantpolymer melanoidin, which if disposed untreated poses a great threat to environment. Microbial decolorization and chemical oxygen demand (COD) reduction was found to be dependent on specific carbon and nitrogen source. Under optimal condition of pH, carbon and nitrogen concentration for each treatment, it was found that Bacillus sp isolated from soil was capable of removing COD (85. 35%) and colour (81.10%) from distillery waste to the maximum extent after 9 days atpH 7 in the medium containing 0.5% peptone, 2% glucose and 10% (v/v), followed by Phanerochaete chrysosporium and lowest reduction was obtained by using native microbial consortium.
SHU 508 A (Levovist)‐Enhanced Doppler Echocardiography Improves the Assessment of Valvular Heart Disease (2001)

In case of an inconclusive unenhanced Doppler echo study, the administration of a left heart contrast agent should be considered. SHU 508 A is especially useful in improving the severity grading of aortic stenosis and aortic regurgitation, while grading of mitral stenosis and mitral regurgitation is less improved.
Strange Cousins (2004)

Research Article| May 01 2004 Strange Cousins mark morton mark morton Search for other works by this author on: This Site PubMed Google Scholar Gastronomica (2004) 4 (2): 6–7. https://doi.org/10.1525/gfc.2004.4.2.6 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation mark morton; Strange Cousins. Gastronomica 1 May 2004; 4 (2): 6–7. doi: https://doi.org/10.1525/gfc.2004.4.2.6 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentGastronomica Search This content is only available via PDF. ©© 2004 BY THE REGENTS OF THE UNIVERSITY OF CALIFORNIA. ALL RIGHTS RESERVED.2004 Article PDF first page preview Close Modal You do not currently have access to this content.
A green fluorescent protein-expressing murine tumour but not its wild-type counterpart is cured by photodynamic therapy (2006)

The ideal cancer treatment should both destroy the primary tumour and at the same time educate the immune system to recognise the tumour as foreign so that distant metastases will also be eradicated. Photodynamic therapy (PDT) involves the i.v. administration of photosensitisers followed by illumination of the tumour with red light producing reactive oxygen species that eventually cause vascular shutdown and tumour cell death by apoptosis and necrosis. Anti-tumour immunity is stimulated after PDT due to the acute inflammatory response, generation of tumour-specific antigens, and induction of heat-shock proteins. Green fluorescent protein (GFP) is used as an optical reporter to noninvasively image the progression of mouse tumours, and in addition, may act as a foreign (jellyfish) antigen. We asked whether GFP-expressing tumours could be used to monitor the response of tumour-bearing mice to PDT, and whether the tumour response differed when a nonimmunogenic tumour cell line was transduced with GFP. We injected RIF-1 or RIF-1 EGFP (stably transduced with a retroviral vector) cells in the leg of C3H/HeN mice and both the cells and tumour grew equally well. We used PDT with benzoporphyrin derivative and a short drug-light interval. There were complete cures and 100% mouse survival of RIF-1 EGFP while RIF-1 wild-type tumours all recurred. Cured mice were resistant to rechallenge with RIF-1 EGFP cells and a rechallenge with wild-type RIF-1 cells grew significantly slower. There was also slower RIF-1 EGFP rechallenge growth but no rejection when RIF-1 EGFP tumours were surgically removed. There was a low rate of PDT cure of tumours when RIF-1 cells were transduced with an empty retroviral vector. The presence of antibodies against EGFP in mouse serum suggests EGFP can act as a foreign antigen and PDT can then stimulate a long-term memory immune response.
Pharmaceuticals and Personal Care Products in U.S. Biosolids (2010)

Since the 1988 U.S. ban on ocean dumping of sewage sludge, the majority of these materials are disposed of on land as biosolids. To provide fundamental data for risk assessment concerning the environment, crop plants, and humans, several studies have been conducted that aimed at quantifying the load of pharmaceuticals and personal care products (PPCPs) as well as other emerging pollutants of concern in biosolids. So far, two large studies exist that analyzed biosolids samples representing the whole U.S. Both sample sets were collected by the U.S. Environmental Protection Agency (EPA) and analyzed by the same contract laboratory that developed the EPA method 1694 for analysis of PPCPs in biosolids and other matrices. The samples were analyzed using liquid chromatography tandem mass spectrometry and quantified using isotope dilution as well as conventional use of internal and external standards. The present meta analysis scrutinizes the findings and approaches of the two studies and puts them in context to potential environmental risks that should be considered. Both the EPA’s Targeted National Sewage Sludge Survey (TNSSS) and the analysis of a comparable sample set collected in 2001 revealed concentrations of several antimicrobials and antibiotics in the mg kg-1 dry weight range. Prevalent contaminants were triclocarban, triclosan, ciprofloxacin and ofloxacin, followed by a number of tetracycline antibiotics. A comparison of the two datasets and study designs showed that combining a large number of individual samples to mega-composite samples is a suitable approach for identifying prevalent contaminants and for obtaining representative mean concentrations. Whereas the use of mega composite samples can result in significant time and cost savings, this study design strategy tends to yield lower numbers of total analytes detected, lower detection frequencies for individual analytes and it limits the detection of spatial (geographical) patterns in analyte occurrence. The findings of both nationwide studies provide a critical data basis for future risk assessment concerning the safety of biosolids application on agricultural and recreational land. Risks of primary concern identified in this work are the promotion of antibiotic resistance in the environment, adverse effects on soil microbial communities and plants, as well as the possibility of direct exposure of consumers to antibiotic residues contained in food crops grown on biosolids amended fields.
Biosensors: A Novel Approach to Detect Food-borne Pathogens. (2018)

Foodborne pathogens affect human health negatively and are known to cause economic losses. Therefore, quick detection of foodborne pathogens and the implementation of measures to ensure their inactivation are of immense significance. Immunological, molecular, and cultural methods are frequently used in the detection of foodborne pathogens. High cost, prolonged analysis times, and the necessity of specialized personnel are some of the disadvantages of these methods. Biosensors are known as analytical devices. The use of biosensors is considered a new approach to quickly detect foodborne pathogens and their toxins. Biosensors, which are capable of converting biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. Different types of biosensor are being employed for detection of pathogenic bacteria. Biosensors are sensitive, fast, economical, reliable, and portable devices, and are used in many fields such as food safety, medicine, pharmacy, measurement of environmental pollution, and the military defense. Electrochemical and optical biosensors and piezoelectric immunosensors are among the most frequently used biosensors in the detection of foodborne pathogens. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in food industry are summarized.
Safety and clinical activity of TTI-621 in combination with doxorubicin in patients with unresectable or metastatic high-grade leiomyosarcoma: Results from the low-dose expansion cohort. (2023)

11508 Background: TTI-621 is a recombinant fusion protein combining the Fc region of human IgG1 with a fragment of human SIRPα, designed as a decoy receptor for CD47 on tumor cells to interrupt CD47-SIRPα signaling and promote macrophage-mediated phagocytosis of tumor cells as well as activation of NK cells. Preclinical studies suggest TTI-621 may enhance the response to doxorubicin in macrophage-rich tumors that express CD47, such as leiomyosarcoma (LMS). A phase 1/2 dose escalation study with high- and low-dose expansion cohorts evaluating TTI-621 + doxorubicin in patients (pts) with advanced LMS is ongoing (NCT04996004); results from the low-dose expansion cohort are presented. Methods: This open-label study enrolled anthracycline-naïve pts aged ≥18 y with evaluable unresectable or metastatic high-grade LMS and ≤1 prior line of therapy for advanced disease. Pts in the low-dose expansion cohort received TTI-621 0.2 mg/kg on Days 1 and 8 in combination with doxorubicin 75 mg/m 2 on Day 1 of 21-day cycles for a maximum 6 cycles, followed by TTI-621 monotherapy on Days 1 and 15 of 28-day cycles until objective disease progression. Primary objectives for the expansion phase were evaluation of safety and investigation of clinical activity via assessment of overall response and clinical benefit rates as defined by RECIST v1.1 criteria. Results: At data cut-off (Oct 3, 2022), 23 pts were evaluable for safety and 20 for clinical response. In the safety population, 39% had received prior treatment for advanced disease. Two pts (9%) experienced Grade ≥3 AE assessed as related to TTI-621 treatment: pancreatitis (n = 1, 4%) and platelet count decreased (n = 1, 4%). Seven pts (30%) experienced Grade ≥3 AE related to the combination of TTI-621 and doxorubicin, including two pts (9%) each with platelet count decreased and white blood cell count decreased and three pts (13%) with neutrophil count decreased. Grade ≥3 doxorubicin-related AEs were more common (n = 17, 74%); most frequent were neutrophil count decreased (n = 8; 35%), white blood cell count decreased (n = 7; 30%), and neutropenia (n = 6; 26%). One pt experienced an infusion-related reaction (Grade 1 chills) assessed as related to TTI-621. No pts attained complete response, but one pt had a complete resolution of all target lesions. Five pts (25%) attained partial response for an overall response rate of 25%; 11 pts (55%) attained stable disease for a disease control rate of 80%. Conclusions: Addition of TTI-621 to doxorubicin showed promising clinical activity and a favorable safety profile among pts with advanced LMS, including those with prolonged exposure to TTI-621 ( &gt; 360 d). Clinical trial information: NCT04996004 .
Regulation of GnRH receptor gene expression in sheep and cattle. (1999)

The GnRH receptor plays a pivotal role in reproduction. This review summarizes current knowledge of the regulation of GnRH receptor gene expression by endocrine factors in sheep and cattle. Expression of the GnRH receptor gene, measured by steady-state amounts of GnRH receptor messenger RNA (mRNA), is maximal during the preovulatory period. The molecular events leading to maximal GnRH receptor gene expression are probably triggered by decreased circulating concentrations of progesterone at luteolysis. Because GnRH is a positive homologous regulator of its own receptor, increased pulsatile GnRH after removal of negative feedback effects of progesterone stimulates expression of the GnRH receptor gene early in the preovulatory period. Oestradiol is also a positive regulator of GnRH receptor gene expression, and increased serum concentrations of oestradiol from developing follicles probably maintain high abundance of GnRH receptor mRNA later in the preovulatory period. Since increased amount of GnRH receptor mRNA precedes maximal numbers of GnRH receptors before the LH surge, increased expression of the GnRH receptor gene is an important mechanism by which maximal sensitivity of gonadotrophs to GnRH is achieved. Future efforts should be directed towards elucidating the molecular mechanisms underlying transcriptional regulation of the GnRH receptor gene in ruminants by endocrine factors.
Use of numerical analysis of nonlinear eddy current problems by finite elements in the determination of parameters of electrical machines with solid iron rotors (1979)

This paper reports on the application of the concept of effective permeability using the finite element technique, to the solution of eddy current problems, and the determination of damping resistances as well as the characteristics of the solid iron rotors in rotating machinery during transients. The formulation is based on the concept of magnetic vector potential in phasor form, coupled with effective permeability which is related to the average magnetic energy throughout an a.c. cycle. The technique is applied to the solid iron rotors of turbogenerators for the determination of equivalent damping resistances, etc., during asynchronous operation. Sample eddy current loss results compare favorably with experimental test data. Instantaneous change in damping resistances during machine transients are obtained and reported on here based on this technique.