gebhart/patrepeval huggingface dataset:
Systemic blockade of P2X3 and P2X2/3 receptors attenuates bone cancer pain behaviour in rats (2010)

Pain remains an area of considerable unmet clinical need, and this is particularly true of pain associated with bone metastases, in part because existing analgesic drugs show only limited efficacy in many patients and in part because of the adverse side effects associated with these agents. An important issue is that the nature and roles of the algogens produced in bone that drive pain-signalling systems remain unknown. Here, we tested the hypothesis that adenosine triphosphate is one such key mediator through actions on P2X3 and P2X2/3 receptors, which are expressed selectively on primary afferent nocioceptors, including those innervating the bone. Using a well-established rat model of bone cancer pain, AF-353, a recently described potent and selective P2X3 and P2X2/3 receptor antagonist, was administered orally to rats and found to produce highly significant prevention and reversal of bone cancer pain behaviour. This attenuation occurred without apparent modification of the disease, since bone destruction induced by rat MRMT-1 carcinoma cells was not significantly altered by AF-353. Using in vivo electrophysiology, evidence for a central site of action was provided by dose-dependent reductions in electrical, mechanical and thermal stimuli-evoked dorsal horn neuronal hyperexcitability following direct AF-353 administration onto the spinal cord of bone cancer animals. A peripheral site of action was also suggested by studies on the extracellular release of adenosine triphosphate from MRMT-1 carcinoma cells. Moreover, elevated phosphorylated-extracellular signal-regulated kinase expression in dorsal root ganglion neurons, induced by co-cultured MRMT-1 carcinoma cells, was significantly reduced in the presence of AF-353. These data suggest that blockade of P2X3 and P2X2/3 receptors on both the peripheral and central terminals of nocioceptors contributes to analgesic efficacy in a model of bone cancer pain. Thus, systemic P2X3 and P2X2/3 receptor antagonists with central nervous system penetration may offer a promising therapeutic tool in treating bone cancer pain.
Antibody response to nonstructural proteins of hepatitis A virus following infection (1993)

The nonstructural proteins of hepatitis A virus (HAV), produced during active virus replication, are alternative antigens that could be used to differentiate disease from inactivated vaccine-induced antibodies. An assay based on immune precipitation of proteins translated from transcripts of the P2 region of viral cDNA was used to evaluate the development of antibodies after natural infection or vaccination. Antibodies against P2 proteins were found in all sera from clinical cases of hepatitis A following the acute phase. Chimpanzees vaccinated with inactivated or cell-adapted HAV had no detectable antibodies against P2 products, either before or after wild type virus challenge. A serosurvey of sera positive for total anti-HAV (HAVAB, Abbott Laboratories, North Chicago) suggested that some individuals had no detectable antibodies to the P2 antigen by immune precipitation. These results were attributed to the lower sensitivity of the immunoprecipitation assay, since antibodies to capsid proteins, as measured by immunoprecipitation, were also not detected in most of these sera.
Network Coding for Distributed Storage Systems (2007)

Peer-to-peer distributed storage systems provide reliable access to data through redundancy spread over nodes across the Internet. A key goal is to minimize the amount of bandwidth used to maintain that redundancy. Storing a file using an erasure code, in fragments spread across nodes, promises to require less redundancy and hence less maintenance bandwidth than simple replication to provide the same level of reliability. However, since fragments must be periodically replaced as nodes fail, a key question is how to generate a new fragment in a distributed way while transferring as little data as possible across the network. In this paper, we introduce a general technique to analyze storage architectures that combine any form of coding and replication, as well as presenting two new schemes for maintaining redundancy using erasure codes. First, we show how to optimally generate MDS fragments directly from existing fragments in the system. Second, we introduce a new scheme called regenerating codes which use slightly larger fragments than MDS but have lower overall bandwidth use. We also show through simulation that in realistic environments, regenerating codes can reduce maintenance bandwidth use by 25% or more compared with the best previous design - a hybrid of replication and erasure codes - while simplifying system architecture.
A Stereotactic Probabilistic Atlas for the Major Cerebral Arteries (2016)

Improved whole brain angiographic and velocity-sensitive MRI is pushing the boundaries of noninvasively obtained cerebral vascular flow information. The complexity of the information contained in such datasets calls for automated algorithms and pipelines, thus reducing the need of manual analyses by trained radiologists. The objective of this work was to lay the foundation for such automated pipelining by constructing and evaluating a probabilistic atlas describing the shape and location of the major cerebral arteries. Specifically, we investigated how the implementation of a non-linear normalization into Montreal Neurological Institute (MNI) space improved the alignment of individual arterial branches. In a population-based cohort of 167 subjects, age 64-68 years, we performed 4D flow MRI with whole brain volumetric coverage, yielding both angiographic and anatomical data. For each subject, sixteen cerebral arteries were manually labeled to construct the atlas. Angiographic data were normalized to MNI space using both rigid-body and non-linear transformations obtained from anatomical images. The alignment of arterial branches was significantly improved by the non-linear normalization (p < 0.001). Validation of the atlas was based on its applicability in automatic arterial labeling. A leave-one-out validation scheme revealed a labeling accuracy of 96 %. Arterial labeling was also performed in a separate clinical sample (n = 10) with an accuracy of 92.5 %. In conclusion, using non-linear spatial normalization we constructed an artery-specific probabilistic atlas, useful for cerebral arterial labeling.
Synthese d'isosteres pyrroliniques de la rilmenidine et de la clonidine (2001)

AU COURS DE CE TRAVAIL, NOUS NOUS SOMMES ATTACHES A OBTENIR DES NOUVEAUX LIGANDS SELECTIFS DES RECEPTEURS DES IMIDAZOLINES PAR RAPPORT AUX SITES DE LIAISON DES IMIDAZOLINES DE SOUS-TYPE 2 ET AUX RECEPTEURS 2-ADRENERGIQUES. DE TELS LIGANDS SONT EN EFFET D'UNE GRANDE UTILITE D'UNE PART POUR LA CARACTERISATION DES RECEPTEURS DES IMIDAZOLINES ET L'ETUDE DE L'EFFET HYPOTENSEUR PROVOQUE PAR LES DROGUES IMIDAZOLINIQUES, MAIS AUSSI POUR OBTENIR DE NOUVEAUX PROTOTYPES D'AGENTS ANTIHYPERTENSEURS CENTRAUX. POUR OBTENIR CES LIGANDS SELECTIFS, NOUS AVONS SYNTHETISE DES ISOSTERES PYRROLINIQUES DES DEUX DROGUES DE REFERENCE QUE SONT LA RILMENIDINE ET LA CLONIDINE. CES SYNTHESES ONT NOTAMMENT CONDUIT A : - UNE SERIE D'ANALOGUES PYRROLINIQUES DE LA RILMENIDINE, SELECTIFS DES RECEPTEURS DES IMIDAZOLINES ET CAPABLES DE FAIRE BAISSER LA PRESSION ARTERIELLE APRES ADMINISTRATION CENTRALE. UN DE CES ANALOGUES, LE LNP 509, S'EST D'ORES ET DEJA AVERE TRES UTILE POUR L'ETUDE DE L'EFFET HYPOTENSEUR DES IMIDAZOLINES. - DEUX ANALOGUES PYRROLINIQUES DE LA CLONIDINE, ORIGINAUX ET HYPOTENSEURS APRES ADMINISTRATION SYSTEMIQUE (LES LNP 541 ET LNP 560) QUI CONSTITUENT DEUX NOUVEAUX PROTOTYPES D'AGENTS ANTIHYPERTENSEURS CENTRAUX. - L'OBTENTION D'UN LIGAND RADIOMARQUE (LNP 911) ET D'UN LIGAND DE PHOTOAFFINITE (LNP 906) TOUS DEUX TRES SELECTIFS ET POSSEDANT UNE AFFINITE SUBNANOMOLAIRE POUR LES RECEPTEURS D'INTERET. CES DEUX MOLECULES CONSTITUENT DEUX NOUVEAUX OUTILS POUR L'ETUDE DES RECEPTEURS DES IMIDAZOLINES.
DNA aptamers selection for breast cancer (2016)

A method of selection of DNA aptamers to breast tumor tissue based on the use of postoperative material has been developed. Breast cancer tissues were used as the positive target; the negative targets included benign tumor tissue, adjacent healthy tissues, breast tissues from mastopathy patients, and also tissues of other types of malignant tumors. During selection a pool of DNA aptamers demonstrating selective binding to breast cancer cells and tissues and insignificant binding to breast benign tissues has been obtained. These DNA aptamers can be used for identification of protein markers, breast cancer diagnostics, and targeted delivery of anticancer drugs.
Prevalência de esofagopatia chagásica no Município de Mambaí, Goiás - Brasil (1987)

Em Mambaí, área endêmica de doença de Chagas, foram realizadas abreugrafias do esôfago com filme de 70mm, em 1.145 indivíduos do sexo masculino e 1.184 dofeminino, de 4 a 87 anos, dos quais 1.006eram soropositivos. Os pacientes foram posicionados em oblíqua anterior direita e tomadas duas abreugrafias: uma imediatamente à ingestão de 75 ml de suspensão baritada e outra 60 segundos após. Setenta e seis (3,2%) indivíduos apresentaram esofagopatia, sendo que 71(7%) entre os 1.006 soropositivos e, 5(0,37%) entre os 1.323 soronegativos. Dos 76 esofagopatas 47(61,8%) eram do sexo masculino e 29(38,1%) do sexo feminino. Segundo a classificação de Rezende e cols 48(63,1%) pertenciam ao grupo I, 18(23,7%) ao grupo II, 5(6,6%) ao grupo III e 5(6,6%) ao grupo IV. Entre os infectados a esofagopatia aumentou progressivamente com a idade, principalmente a partir dos 30 anos, alcançando a prevalência de 21,5% após os 59 anos.
Aperiodic property of interval polynomials (1991)

An examination is made of the aperiodic property of interval polynomials. The authors also characterize the zero locations of aperiodic interval polynomials. Examples are given that clarify the points made.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Matched subspace detectors (2002)

Studies the problem of detecting subspace signals in subspace interference and broadband noise. The authors derive the generalized likelihood ratio (GLR) and establish its invariances. Then then prove that the GLR test (GLRT) is the uniformly most powerful invariant detector, establishing once and for all its optimality.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Chemical Sensors for Fuel Cell Systems (2003)

Power generation systems based on fuel cells requir e sensors to detect numerous volatiles at extremely l ow levels (0.1 ppm) in the presence of hydrogen and ot her gaseous components (N 2, CO 2, methane and steam). In the case of PEM fuel cells presence of carbon monoxide and sulfur-bearing species in the gas stream is undesir able, as these are known to poison the Pt anode. The automotive fuel cell application requires a sensor to detect l ow levels of carbon monoxide (~1ppm CO) in order to control the fuel processor and sulfur (~0.1ppm) in order to pro tect the anode. However, their monitoring in a hydrogen-ric h reformate (~40% hydrogen) is a challenging task and standard combustion based sensors will no longer work in environments lacking oxygen. The measurement becomes more daunting in presence of appreciable amount of carbon dioxide, and water vapor in the reformate ga s. Figure 1 shows a schematic of the multiple catalyti c steps in fuel processing.
Interferência de rna (rnai) para redução de ácido fítico em soja (Glycine max (L.) MERRIL). (2004)

Made available in DSpace on 2011-04-09T13:06:53Z (GMT). No. of bitstreams: 1\ntales2004.pdf: 638378 bytes, checksum: 36ecd45d3dde66a3f34bb483b755a0a0 (MD5)\n Previous issue date: 2005-06-09
Using Light Microscopy and Liquid Chromatography Tandem Mass Spectrometry for Qualitative and Quantitative Control of a Combined Three-Herb Formulation in Different Preparations (2016)

<i>Artemisia capillaries</i> Thunb, <i>Gardenia jasminoides</i> Ellis, and <i>Rheum officinale</i> Baill have been combined to treat jaundice for thousands of years. Studies have revealed that these herbs induce anti-hepatic fibrosis and anti-hepatic apoptosis and alleviate hepatic oxidative stress. This study aims to determine the quality and quantity of an herbal formulation (Chinese name: Yin-Chen-Hao-Tang) using physical and chemical examinations. Physical examination of Yin-Chen-Hao-Tang in pharmaceutical herbal products, raw fiber powders, and decoction preparations was performed using Congo red and iodine-potassium staining. A sensitive and validated method employing ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed to simultaneously quantify the bioactive compounds scoparone, geniposide, and rhein in the Yin-Chen-Hao-Tang formulation in different preparations. Physical examination indicated that cellulose fibers with irregular round shapes were present in the pharmaceutical herbal products. The developed UHPLC-MS/MS method showed good linearity and was well validated. The quantification results revealed that the decoction preparations had the highest amounts of geniposide and rhein. Scoparone appeared in pharmaceutical herbal products from two manufacturers. This experiment provides a qualitative and quantitative method using physical and chemical examinations to test different preparations of herbal products. The results provide a reference for clinical herbal product preparations and further pharmacokinetic research.
Pathogen effector protein screening in yeast identifies Legionella factors that interfere with membrane trafficking (2005)

Legionella pneumophila invades and replicates intracellularly in human and protozoan hosts. The bacteria use the Icm/Dot type IVB secretion system to translocate effectors that inhibit phagosome maturation and modulate host vesicle trafficking pathways. To understand how L. pneumophila modulates organelle trafficking in host cells, we carried out pathogen effector protein screening in yeast, identifying L. pneumophila genes that produced membrane trafficking [vacuole protein sorting (VPS)] defects in yeast. We identified four L. pneumophila DNA fragments that perturb sorting of vacuolar proteins. Three encode ORFs of unknown function that are translocated via the Icm/Dot transporter from Legionella into macrophages. VPS inhibitor protein (Vip) A is a coiled-coil protein, VipD is a patatin domain-containing protein, and VipF contains an acetyltransferase domain. Processing studies in yeast indicate that VipA, VipD, and VipF inhibit lysosomal protein trafficking by different mechanisms; overexpressing VipA has an effect on carboxypeptidase Y trafficking, whereas VipD interferes with multivesicular body formation at the late endosome and endoplasmic reticulum-to-Golgi body transport. Such differences highlight the multiple strategies L. pneumophila effectors use to subvert host trafficking processes. Using yeast as an effector gene discovery tool allows for a powerful, genetic approach to both the identification of virulence factors and the study of their function.
Mapping the Melanoma Plasma Proteome (MPP) Using Single-Shot Proteomics Interfaced with the WiMT Database (2021)

Plasma analysis by mass spectrometry-based proteomics remains a challenge due to its large dynamic range of 10 orders in magnitude. We created a methodology for protein identification known as Wise MS Transfer (WiMT). Melanoma plasma samples from biobank archives were directly analyzed using simple sample preparation. WiMT is based on MS1 features between several MS runs together with custom protein databases for ID generation. This entails a multi-level dynamic protein database with different immunodepletion strategies by applying single-shot proteomics. The highest number of melanoma plasma proteins from undepleted and unfractionated plasma was reported, mapping >1200 proteins from >10,000 protein sequences with confirmed significance scoring. Of these, more than 660 proteins were annotated by WiMT from the resulting ~5800 protein sequences. We could verify 4000 proteins by MS1t analysis from HeLA extracts. The WiMT platform provided an output in which 12 previously well-known candidate markers were identified. We also identified low-abundant proteins with functions related to (i) cell signaling, (ii) immune system regulators, and (iii) proteins regulating folding, sorting, and degradation, as well as (iv) vesicular transport proteins. WiMT holds the potential for use in large-scale screening studies with simple sample preparation, and can lead to the discovery of novel proteins with key melanoma disease functions.
Mechanical testing of graphite-epoxy composite tubes (1986)

The in-plane elastic moduli of a unidirectional graphite-epoxy laminate were evaluated by loading tubular specimens in tension, torsion, and internal pressure. Similarly, ''effective'' moduli for crossply tubes of a prescribed lay-up were evaluated, then compared with predictions based upon plate lamination theory and the unidirectional moduli. Fixture design and software for test control, data acquisition, and data reduction are documented.
Qualitative analysis and synthesis of a class of neural networks (1988)

The dynamic properties of a class of neural networks (which includes the Hopfield model as a special case) are investigated by studying the qualitative behavior of equilibrium points. The results fall into one of two categories: results pertaining to analysis (e.g., stability properties of an equilibrium, asymptotic behavior of solutions, etc.) and results pertaining to synthesis (e.g. the design of a neural network with prespecified equilibrium points which are asymptotically stable). Most (but not all) of the results presented are global, and their applicability is demonstrated by an example.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Vibrational Raman Optical Activity Characterization of Poly(l-proline) II Helix in Alanine Oligopeptides (2004)

A vibrational Raman optical activity (ROA) study of a series of alanine peptides in aqueous solution is presented. The seven-alanine peptide Acetyl-OOAAAAAAAOO-Amide (OAO), recently shown by NMR and UVCD to adopt a predominantly poly(l-proline II) (PPII) helical conformation in aqueous solution, gave an ROA spectrum very similar to that of disordered poly(l-glutamic acid) which has long been considered to adopt the PPII conformation, both being dominated by a strong positive extended amide III ROA band at approximately 1319 cm-1 together with weak positive amide I ROA intensity at approximately 1675 cm-1. A series of alanine peptides Ala2-Ala6 studied in their cationic states in aqueous solution at low pH displayed ROA spectra which steadily evolved toward that of OAO with increasing chain length. As well as confirming that alanine peptides can support the PPII conformation in aqueous solution, our results also confirm the previous ROA band assignments for PPII structure, thereby reinforcing the foundation for ongoing ROA studies of unfolded and partially folded proteins.
Analysis of Interdicted Samples From Australia (Final Report) (2021)

Abstract not provided.
Synthesis and structural characterization of(1R, 4S, 5S)-6- cyclohexyl-4-[(1R, 2S, 5R)-2-isopropyl-5-methyl-cyclohexyloxy]-3- oxa-6-aza-bycyclo[3.1.0]hexan-2-one (2013)

The title compound,a novel chiral arziridine-butyrolactone,was synthesized through the Michael addition/intramolecular nucleophilic substitution of cyclohexylamine with epimer 3-bromo-5-[(1R,2S,5R)-2-isopropyl-5-methylcyclohexyloxy]furan-2(5H)-one followed by column chromatography.The yields was 17%.The structure was characterized through IR﹑1H NMR﹑13C NMR and HRMS.
Phosphorus Substitution in Zeolite Frameworks (1974)

Zeolites containing phosphorus in the tetrahedral site in the framework have been synthesized. Phosphorus incorporation in a variety of structural types of zeolite frameworks has been achieved: analcime, phillipsite, chabazite, Type A zeolite, Type L zeolite, and Type B (P) zeolite. The syntheses and properties of some of the new aluminosilicophosphate zeolites are described. The synthesis technique involves gel crystallization where incorporation of phosphorus is accomplished by controlled copolymerization and coprecipitation of all the framework component oxides, aluminate, silicate, and phosphate, into a relatively homogeneous gel phase. Subsequent crystallization of the gel is carried out at temperatures in the region of 80° to 210°C. Proof and mechanism of framework substitution of phosphorus is based on electron microprobe analysis, infrared spectroscopy, and other characterization.
Sequential implication of the mental retardation proteins ARHGEF6 and PAK3 in spine morphogenesis (2006)

The biological mechanisms underlying the mental retardation associated with mutation of the ARHGEF6 gene, a Rac1/Cdc42 exchange factor, are still unknown, although defects in the plasticity of synaptic networks have been postulated. We have cloned the rat ARHGEF6 gene and investigated, using a transfection approach, its involvement in spine morphogenesis and its relationship to p21-activated kinase 3 (PAK3). We found that expression of tagged ARHGEF6 in hippocampal slice cultures shows a punctate staining in dendritic spines that colocalizes with PSD95. Over-expression of ARHGEF6, of PAK3 or constitutively active PAK3 did not alter spine morphology. By contrast, knockdown of ARHGEF6 using a siRNA approach resulted in abnormalities in spine morphology similar to those reported with knockdown of PAK3. This phenotype could be rescued through co-expression of a constitutively active PAK3 protein, but not with wild-type PAK3. Together, these results indicate that ARHGEF6 is localized in dendritic spines where it contributes to regulate spine morphogenesis probably by acting through a downstream activation of PAK3. Similar mechanisms are thus likely to underlie the mental retardation induced by mutations of ARHGEF6 and PAK3.
Motor Performance and Lower Extremity Fat Fraction in Myotonic Dystrophies Type 1 and Type 2, and Controls (P5-8.014) (2023)

To characterize lower extremity muscle fat fraction (MFF) in patients with myotonic dystrophy type 1 (DM1), myotonic dystrophy type 2 (DM2), and controls. Additionally, we examine the associations between MFF and disease-specific endpoints.