gebhart/patrepeval huggingface dataset:
Monte Carlo dosimetry of the selectSeed interstitial brachytherapy seed (2001)

This work provides full dosimetric data for the new selectSeed 125I prostate seed source to be distributed by Nucletron B.V. The AAPM TG-43 dosimetric formalism and the new 1999 NIST air kerma strength calibration standard have been followed. Air kerma strength, dose rate constant, radial dose functions, anisotropy functions, and anisotropy factors were calculated using Monte Carlo simulation. Corresponding calculations were also performed for the commercially available 6711 seed source, which is of similar design, for reasons of comparison. The calculated dose rate constant of the selectSeed was 0.954+/-0.005 cGy h(-1) U(-1) compared to 0.953+/-0.005 cGy h(-1) U(-1) for the 6711 source design. The latter value for the 6711 source suggests that the correction factor proposed by NIST for conversion of dose rate constants to the new 1999 NIST calibration standard may be overestimated by 2-3%. Radial dose functions of the two sources were found in good agreement for radial distances up to 4 cm, the selectSeed being less penetrating at greater radial distances (approximately 4% at 10 cm). The selectSeed source presents similar anisotropy characteristics with the 6711 source design. For both source designs, a distance and polar angle dependent discontinuity of anisotropy function values was observed owing to the dose contribution of radioactivity distributed on the ends of the cylindrical source cores. Variation of dosimetric parameters with possible variation in radioactive silver halide coating thickness of the silver source core of the new source was also investigated.
Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome (2008)

The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.
Coordinated Regulation of Synaptic Plasticity at Striatopallidal and Striatonigral Neurons Orchestrates Motor Control (2015)

The basal ganglia play a critical role in shaping motor behavior. For this function, the activity of medium spiny neurons (MSNs) of the striatonigral and striatopallidal pathways must be integrated. It remains unclear whether the activity of the two pathways is primarily coordinated by synaptic plasticity mechanisms. Using a model of Parkinson's disease, we determined the circuit and behavioral effects of concurrently regulating cell-type-specific forms of corticostriatal long-term synaptic depression (LTD) by inhibiting small-conductance Ca(2+)-activated K(+) channels (SKs) of the dorsolateral striatum. At striatopallidal synapses, SK channel inhibition rescued the disease-linked deficits in endocannabinoid (eCB)-dependent LTD. At striatonigral cells, inhibition of these channels counteracted a form of adenosine-mediated LTD by activating the ERK cascade. Interfering with eCB-, adenosine-, and ERK signaling in vivo alleviated motor abnormalities, which supports that synaptic modulation of striatal pathways affects behavior. Thus, our results establish a central role of coordinated synaptic plasticity at MSN subpopulations in motor control.
Simian Hemorrhagic Fever (SHF) Virus. Phase 3 (1993)

Abstract : Phase III of this contract required that we twice-clone selected simian hemorrhagic fever (SHF) virus-specific hybridoma cultures, expand two clones from each clone as well as 50 ml of supernatant fluid from cultures of each clone. RA I, Hemorrhagic fevers, Virus.
Conception de capteurs acoustiques basse fréquence pour la caractérisation de milieux complexes (2010)

Habilitation à diriger des recherches en Electronique, Université de Valenciennes et du Hainaut-Cambrésis, 29 novembre
Injection-Locked Millimeter Wave Frequency Divider Utilizing Optoelectronic Oscillator Based Optical Frequency Comb (2019)

We propose and experimentally demonstrate a photonic millimeter-wave frequency divider based on a super-harmonic injection-locked optoelectronic oscillator (OEO) and an optical frequency comb. The optical frequency comb generator is incorporated into an OEO loop to create broadband comb lines with low noise. By injecting a millimeter-wave with frequency around N times of the fundamental oscillating frequency f0 of the OEO, the injection signal can be down-converted to an intermediate frequency (IF) signal close to the oscillating frequency of the OEO. The free running OEO is synchronized with the IF signal via an injection locking mechanism. Consequently, it forms an injection-locked frequency divider as the frequency ratio between the injection signal and OEO's output signal is precisely equal to N. We carry out an experiment to divide the 45 GHz signal into 7.5 GHz and the experimental results agree well with the theoretical analysis. By changing the frequency of the injection signal, division ratio from 2 to 5 is also demonstrated on one setup. Due to the broadband spectra of the optical comb and low phase noise characteristics of the OEO, it is potential to realize very large division ratio and low phase noise for multiple input frequencies mf <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> .
T-DNA Activation Tagging (2003)

T-DNA activation tagging is a method to generate dominant mutations in plants or plant cells by random insertion of a T-DNA carrying constitutive enhancer elements, which can cause transcriptional activation of flanking plant genes. The method consists of generating a large number of transformed plants or plant cells using a specialized T-DNA construct, followed by selection for the desired phenotype. Subsequently, the activated plant gene is rescued from selected mutant transformants for further functional analysis. Since the exact procedure depends on the plant material and the selected phenotype, this chapter describes one specific example of T-DNA activation tagging of suspension-cultured cells, including, where possible, cross-references to more general applications of the technique.
Mbnl2 loss alters novel context processing and impairs object recognition memory (2023)

Patients with myotonic dystrophy type I (DM1) demonstrate visuospatial dysfunction and impaired performance in tasks requiring recognition or memory of figures and objects. In DM1, CUG expansion RNAs inactivate the muscleblind-like (MBNL) proteins. We show that constitutive Mbnl2 inactivation in <i>Mbnl2</i><sup><i>ΔE2/ΔE2</i></sup> mice selectively impairs object recognition memory in the novel object recognition test. When exploring the context of a novel arena in which the objects are later encountered, the <i>Mbnl2</i><sup><i>ΔE2/ΔE2</i></sup> dorsal hippocampus responds with a lack of enrichment for learning and memory-related pathways, mounting instead transcriptome alterations predicted to impair growth and neuron viability. In <i>Mbnl2</i><sup><i>ΔE2/ΔE2</i></sup> mice, saturation effects may prevent deployment of a functionally relevant transcriptome response during novel context exploration. Post-novel context exploration alterations in genes implicated in tauopathy and dementia are observed in the <i>Mbnl2</i><sup><i>ΔE2/ΔE2</i></sup> dorsal hippocampus. Thus, MBNL2 inactivation in patients with DM1 may alter novel context processing in the dorsal hippocampus and impair object recognition memory.
A potentially functional polymorphism in the promoter region of miR‐34b/c is associated with an increased risk for primary hepatocellular carcinoma (2010)

The miR-34 family members are direct transcriptional targets of tumor suppressor p53, and loss of miR-34 function can impair p53-mediated cell cycle arrest and apoptosis. A potentially functional SNP rs4938723 (T > C) was found in the promoter region of pri-miR-34b/c (423 bp from the transcription start site), located in the CpG island and might affect transcription factor GATA binding and therefore pri-miR-34b/c expression. In our study, we hypothesized that SNPs miR-34b/c rs4938723 and TP53 Arg72Pro may independently or jointly contribute to primary hepatocellular carcinoma (HCC) susceptibility. We then genotyped the 2 SNPs in a case-control study of 501 patients with primary HCC and 548 cancer-free controls in a Chinese population. We observed that the variant genotypes of miR-34b/c rs4938723 were associated with significantly increased HCC risks compared with the wild-type TT genotype (adjusted OR = 1.37, 95% CI =1.06-1.78 for TC; OR = 1.53, 95% CI = 1.02-2.31 for CC and OR = 1.40, 95% CI = 1.10-1.80 for TC/CC). Furthermore, we found a significant interaction between alcohol drinking and SNP rs4938723 on HCC risk (p = 0.05 for multiplicative and p = 0.01 for additive interaction). However, we did not find any main effect of TP53 Arg72Pro on HCC risk in this population. These findings indicate that the potentially functional SNP rs4938723 in the promoter region of pri-miR-34b/c may contribute to the susceptibility of HCC in this Chinese population.
Preparation of multilayered materials in cross-section for in situ TEM tensile deformation studies (1997)

The success of in situ transmission electron microscopy experimentation is often dictated by proper specimen preparation and sample design procedures. We have developed a novel technique permitting the production of tensile specimens of multilayered films in cross-section for in situ deformation studies. Of primary, importance in the development of this technique is the production of an electron transparent micro-gauge section. This micro-gauge section predetermines the position at which plastic deformation, crack nucleation and growth, and failure are observed. In short, we report in detail, a unique combination of specimen preparation procedural steps and the design of a multilayer foil sample. The ability of these procedures to facilitate the success of in situ TEM tensile studies of layered materials in cross-section is demonstrated using a Cu-Zr multilayer foil.
Theoretical Study of Hydration of Cyanamide and Carbodiimide (2003)

The isomerization reaction cyanamide → carbodiimide in vacuo and in the presence of up to six water molecules has been investigated by means of DFT and MP2 calculations, using a flexible basis set. The reliability of such methods has been checked against results of coupled cluster calculations on isolated molecules. The effect of water molecules has also been investigated in the case of the hydrolysis reaction of cyanamide and carbodiimide, leading to isourea. The number of water molecules considered is large enough to give results converged in activation and hydration energies. In addition to the water molecules explicitly described, the effect of water bulk solvent is taken into account according to the polarizable continuum model. The results show that the direct hydrolysis of H2NCN is hindered by an activation energy much higher than that for HNCNH, which in turn can be obtained from cyanamide with a relatively easy process.
<title>Performance characteristics of fiber optic lignin concentration sensor</title> (1995)

In the paper-making process, wood chips are cooked to form a pulp, which is subsequently washed, bleached, and rolled into sheets. The mechanical strength, uniformity, and amount of bleach necessary to whiten the paper all depend on the lignin concentration in the wood pulp. In this presentation, we describe the developement of a laser-induced fluorescence method to measure lignin concentration in wood pulp, application of the method to characterize hundreds of different pulp samples obtained from different kinds of wood, and correlation of the results with industry-standard wet chemical Kappa number tests. We report the results of detailed characterization tests performed to determine the sensitivity and repeatability of the method and to quantify cross-sensitivity to other variables such as temperature, consistency, and pH.
Heterogeneity of Response to Iron-Based Metallodrugs in Glioblastoma Is Associated with Differences in Chemical Structures and Driven by FAS Expression Dynamics and Transcriptomic Subtypes (2021)

Glioblastoma (GBM) is the most frequent and deadliest primary brain cancer in adults, justifying the search for new treatments. Some members of the iron-based ferrocifen family have demonstrated a high cytotoxic effect on various cancer cell lines via innovative mechanisms of action. Here, we evaluated the antiproliferative activity by wst-1 assay of six ferrocifens in 15 molecularly diverse GBM patient-derived cell lines (PDCLs). In five out of six compounds, the half maximal inhibitory concentration (IC<sub>50</sub>) values varied significantly (10 nM < IC<sub>50</sub> < 29.8 µM) while the remaining one (the tamoxifen-like complex) was highly cytotoxic against all PDCLs (mean IC<sub>50</sub> = 1.28 µM). The pattern of response was comparable for the four ferrocifens bearing at least one phenol group and differed widely from those of the tamoxifen-like complex and the complex with no phenol group. An RNA sequencing differential analysis showed that response to the diphenol ferrocifen relied on the activation of the Death Receptor signaling pathway and the modulation of FAS expression. Response to this complex was greater in PDCLs from the Mesenchymal or Proneural transcriptomic subtypes compared to the ones from the Classical subtype. These results provide new information on the mechanisms of action of ferrocifens and highlight a broader diversity of behavior than previously suspected among members of this family. They also support the case for a molecular-based personalized approach to future use of ferrocifens in the treatment of GBM.
Biotransformation of atrazine in transgenic tobacco cell culture expressing human P450 (2003)

Plant cell cultures in which the appropriate P450 cDNA is introduced are expected to metabolise certain pesticides in large quantities. Two species of human P450 (CYP1A1 and CYP1A2) were introduced into tobacco cells (Nicotiana tabacum L) by Agrobacterium-mediated transformation. The transgenic plant cell cultures were selected by combination of kanamycin-resistance, 7-ethoxycoumarin O-de-ethylase activity, PCR and Western blot analysis. For metabolism studies, 14C-labelled atrazine was used as a model substance. The metabolites de-ethylatrazine and de-isopropylatrazine were found in the control culture as well as in the transgenic culture, whereas the non-phytotoxic metabolite de-ethyl-de-isopropylatrazine was found only in the transgenic cell cultures. The results showed that both foreign enzymes CYP1A1 and CYP1A2 catalyse N-dealkylation of atrazine. However, CYP1A2 exhibited a higher conversion rate than CYP1A1. In a time-course study the enzyme CYP1A2 catalysed predominantly N-de-ethylation followed by de-isopropylation. The extent of metabolism was considerably higher than in non-transformed cell cultures. The transgenic cell cultures can therefore be suitable tools for the production of large quantities of primary oxidised pesticide metabolites.
Dual-LP_11 mode 4x4 MIMO-OFDM transmission over a two-mode fiber (2011)

We report successful transmission of dual-LP(11) mode (LP(11a) and LP(11b)), dual polarization coherent optical orthogonal frequency-division multiplexing (CO-OFDM) signals over two-mode fibers (TMF) using all-fiber mode converters. Mode converters based on mechanically induced long-period grating with better than 20 dB extinction ratios are realized and used for interfacing single-mode fiber transmitter and receivers to the TMF. We demonstrate that by using 4×4 MIMO-OFDM processing, the random coupling of the two LP(11) spatial modes can be successfully tracked and equalized with a one-tap frequency-domain equalizer. We achieve successful transmission of 35.3 Gb/s over 26-km two-mode fiber with less than 3 dB penalty.
Enhanced Water Purification: A Single Atom Makes a Difference (2009)

The aluminum Keggin polycation (Al13) has been identified as an effective specie for neutralization and coagulation of anionic contaminants in water. In this study, we compare efficacy of the aluminum Keggin-ion to the analogues containing a single Ga-atom or single Ge-atom (GaAl12 and GeAl12, respectively) substituted into the center of the polycation in water-treatment studies. We investigated removal of bacteriophage (model viruses), Cryptosporidium, dissolved organic carbon (DOC), and turbidity. In every study, the order of contaminant removal efficacy trends GaAl12 > Al13 > GeAl12. By ESI MS (electrospray ionization mass spectrometry), we noted the GaAl12 deprotonates least of the three aluminum polycations, and thus probably carries the highest charge, and also optimal contaminant-neutralization ability. The ESI MS studies of the aluminum polycation solutions, as well as solid-state characterization of their resulting precipitates both reveal some conversion of Al13 to larger polycations, Al30 for instance. The GaAl12 does not show any evidence for this alteration that is responsible for poor shelf life of commercial prehydrolyzed aluminum coagulants such as polyaluminum chloride. Based on these studies, we conclude that substitution of a single Ga-atom in the center of the aluminum Keggin polycation produces an optimal water-treatment product due to enhanced shelf life and efficacy in neutralization of anionic contaminants.
Renovascular disease, microcirculation, and the progression of renal injury: role of angiogenesis (2011)

Emerging evidence supports the pivotal role of renal microvascular disease as a determinant of tubulo-interstitial and glomerular fibrosis in chronic kidney disease. An intact microcirculation is vital to restore blood flow to the injured tissues, which is a crucial step to achieve a successful repair response. The purpose of this review is to discuss the impact and mechanisms of the functional and structural changes of the renal microvascular network, as well as the role of these changes in the progression and irreversibility of renal injury. Damage of the renal microcirculation and deterioration of the angiogenic response may constitute early steps in the complex pathways involved in progressive renal injury. There is limited but provocative evidence that stimulation of vascular proliferation and repair may stabilize renal function and slow the progression of renal disease. The feasibility of novel potential therapeutic interventions for stabilizing the renal microvasculature is also discussed. Targeted interventions to enhance endogenous renoprotective mechanisms focused on the microcirculation, such as cell-based therapy or the use of angiogenic cytokines have shown promising results in some experimental and clinical settings.
Heat exchange during encapsulation in a Chemical-Warfare Agent Protective Patient Wrap in four hot environments. Technical report (1987)

The purpose of this study was to determine safe encapsulation time limits in four hot environments including a simulated solar heat load and thereby generate an equation predicting safe time limits for hot environments. Eight male subjects were studied during encapsulation in a Chemical Warfare Agent Protective Patient Wrap in each of four environments. Rectal temperature, mean skin temperature, mean body temperature air temperature and dew point temperature within the wrap and wrap temperature were measured every minute. Metabolic rate was measured during encapsulation by partitional calorimetry. The data shows that safe encapsulation time is severely limited in Hot/Dry and Hot/Wet environments when a solar heat load is included.
High isolation diplexer using quarter-wavelength resonator filter (2012)

A microstrip diplexer with very high isolation is presented. The diplexer consists of two compact filters with quarter-wavelength resonators coupled by the metallised vias. Each metallised via is inductive and behaves as a K-inverter. This type of filter has two adjustable transmission zeros on each side of the passband, which can be shifted by changing the coupling coefficient between the source and load. By locating the transmission zero of one channel at the passband of the other channel, very high isolation of the diplexer can be achieved. Simulated results of the fabricated diplexer agree well with measured results.
Dicarbazolyldicyanobenzenes as Thermally Activated Delayed Fluorescence Emitters: Effect of Substitution Position on Photoluminescent and Electroluminescent Properties (2013)

Abstract We demonstrate two efficient blue-green thermally activated delayed fluorescence (TADF) compounds comprising a dimeric phenylcarbazole and four cyano substituents on the phenyl rings. A comparison of the 2,6-dicyano-substituted derivative (26IPNDCz) with the 3,5-dicyano-substituted derivative (35IPNDCz) shows that 26IPNDCz provides a larger dihedral angle and a lower decrease in the energy difference between the first singlet and triplet excited states (ΔEST) and the TADF lifetime. An organic light-emitting diode based on 26IPNDCz afforded an external quantum efficiency of 10% with reduced efficiency roll-off.
(Z)-3-(3,4-Dimethoxyphenyl)-2-(4-methoxyphenyl)acrylonitrile (2006)

A new dipolarophile used in the construction of bioactive heterocycles, (Z)-3-(3,4-dimethoxy­phen­yl)-2-(4-methoxy­phen­yl)acrylonitrile, C18H17NO3, has been synthesized by base-catalysed reaction of 3,4-dimethoxy­benzaldehyde with (4-methoxy­phen­yl)acetonitrile. The olefinic bond has Z geometry and the mol­ecules are linked by C—H⋯O and C—H⋯N hydrogen bonds.
Fuzzy-Skyhook Control for Active Suspension Systems Applied to a Full Vehicle Model (2012)

Nowadays, most modern vehicles are equipped with controlled suspension systems for improving the vehicle&#13;\nride comfort. Therefore, this paper is concerned with a theoretical study for the ride comfort performance of the&#13;\nvehicle. The theoretical investigation includes a suggestion of an active suspension system controller using&#13;\nfuzzy-skyhook control theory, which offers new opportunities for the improvement of vehicle ride performance. The&#13;\nride comfort of the active suspension system has been evaluated using a 7 degree of freedom full vehicle&#13;\nmathematical model. The simulation results are presented in the time and frequency domain, also in terms of RMS&#13;\nvalues, and it’s shown that the proposed active suspension system with fuzzy-skyhook control improved the vehicle&#13;\nride quality in terms of body acceleration, suspension working space and dynamic tyre load in comparison with the&#13;\npassive and skyhook suspension systems.