gebhart/patrepeval huggingface dataset:
Actin in the acrosome of the spermatozoa of the crab, Cancer pagurus L. (decapoda, crustacea) (1994)

The cytoskeletal protein actin was identified in the mature spermatozoon of the European edible crab, Cancer pagurus Linnaeus, by indirect immunofluorescence with monoclonal and polyclonal anti-actin antibodies, fluorescent phalloidin, and DNAase I. The actin was localized in two distinct concentric rings within the acrosome vesicle of the spermatozoon and appeared to correlate with the internal zonation of the vesicle. Modifications of the fluorescent pattern for actin were observed in sperm cells which were undergoing changes associated with the acrosome reaction. In these cases, fluorescent staining was observed in the nucleocytoplasm immediately subjacent to the perforatorial column and sometimes in the perforatorial column within the acrosome vesicle. Equally intense fluorescence was observed in an apical perforatorial projection. SDS-PAGE of C. pagurus sperm confirmed the presence of actin in the cells. A single band of actin (approximately 43 kDa) comigrated with rabbit muscle actin when immunoblotted onto nitrocellulose with mouse monoclonal anti-actin. The actin-associated cytoskeletal proteins alpha-actinin, tropomyosin, and spectrin were also identified within the spermatozoon of C. pagurus using specific polyclonal antibodies, but their presence was not confirmed by SDS-PAGE and immunoblotting.
Supplementary Figure Legends from Systematic <i>In Vivo</i> Inactivation of Chromatin-Regulating Enzymes Identifies Setd2 as a Potent Tumor Suppressor in Lung Adenocarcinoma (2023)

&lt;p&gt;Figure legends for supplementary figures.&lt;/p&gt;
Photosynthetic Control of Atmospheric Carbonyl Sulfide During the Growing Season (2008)

Climate models incorporate photosynthesis-climate feedbacks, yet we lack robust tools for large-scale assessments of these processes. Recent work suggests that carbonyl sulfide (COS), a trace gas consumed by plants, could provide a valuable constraint on photosynthesis. Here we analyze airborne observations of COS and carbon dioxide concentrations during the growing season over North America with a three-dimensional atmospheric transport model. We successfully modeled the persistent vertical drawdown of atmospheric COS using the quantitative relation between COS and photosynthesis that has been measured in plant chamber experiments. Furthermore, this drawdown is driven by plant uptake rather than other continental and oceanic fluxes in the model. These results provide quantitative evidence that COS gradients in the continental growing season may have broad use as a measurement-based photosynthesis tracer.
Analysis of masked mutations in (2016)

Familial adenomatous polyposis (FAP) is an autosomal-dominant disease characterized by the develop- ment of hundreds of adenomatous polyps of the colorectum. Approximately 80% of FAP patients can be shown to have truncating mutations of the APC gene. To determine the cause of FAP in the other 20% of patients, MAMA (monoallelic mutation analysis) was used to independently examine the status of each of the two APC alleles. Seven of nine patients analyzed were found to have significantly reduced expression from one of their two alleles whereas two patients were found to have full-length expression from both alleles. We conclude that more than 95% of patients with FAP have inactivating mutations in APC and that a combination of MAMA and standard genetic tests will identify APC abnormalities in the vast majority of such patients. That no APC expression from the mutant allele is found in some FAP patients argues strongly against the requirement for dominant negative effects of APC mutations. The results also suggest that there may be at least one additional gene, besides APC, that can give rise to
Accelerated Repetitive Transcranial Magnetic Stimulation to Treat Major Depression: The Past, Present, and Future (2023)

Repetitive transcranial magnetic stimulation (rTMS) is an effective and evidence-based therapy for treatment-resistant major depressive disorder. A conventional course of rTMS applies 20-30 daily sessions over 4-6 weeks. The schedule of rTMS delivery can be accelerated by applying multiple stimulation sessions per day, which reduces the duration of a treatment course with a predefined number of sessions. Accelerated rTMS reduces time demands, improves clinical efficiency, and potentially induces faster onset of antidepressant effects. However, considerable heterogeneity exists across study designs. Stimulation protocols vary in parameters such as the stimulation target, frequency, intensity, number of pulses applied per session or over a course of treatment, and duration of intersession intervals. In this article, clinician-researchers and neuroscientists who have extensive research experience in accelerated rTMS synthesize a consensus based on two decades of investigation and development, from early studies ("Past") to contemporaneous theta burst stimulation, a time-efficient form of rTMS gaining acceptance in clinical settings ("Present"). We propose descriptive nomenclature for accelerated rTMS, recommend avenues to optimize therapeutic and efficiency potential, and suggest using neuroimaging and electrophysiological biomarkers to individualize treatment protocols ("Future"). Overall, empirical studies show that accelerated rTMS protocols are well tolerated and not associated with serious adverse effects. Importantly, the antidepressant efficacy of accelerated rTMS appears comparable to conventional, once daily rTMS protocols. Whether accelerated rTMS induces antidepressant effects more quickly remains uncertain. On present evidence, treatment protocols incorporating high pulse dose and multiple treatments per day show promise and improved efficacy.
Host Response and Neo-Tissue Development during Resorption of a Fast Degrading Supramolecular Electrospun Arterial Scaffold (2018)

In situ vascular tissue engineering aims to regenerate vessels "at the target site" using synthetic scaffolds that are capable of inducing endogenous regeneration. Critical to the success of this approach is a fine balance between functional neo-tissue formation and scaffold degradation. Circulating immune cells are important regulators of this process as they drive the host response to the scaffold and they play a central role in scaffold resorption. Despite the progress made with synthetic scaffolds, little is known about the host response and neo-tissue development during and after scaffold resorption. In this study, we designed a fast-degrading biodegradable supramolecular scaffold for arterial applications and evaluated this development in vivo. Bisurea-modified polycaprolactone (PCL2000-U4U) was electrospun in tubular scaffolds and shielded by non-degradable expanded polytetrafluoroethylene in order to restrict transmural and transanastomotic cell ingrowth. In addition, this shield prevented graft failure, permitting the study of neo-tissue and host response development after degradation. Scaffolds were implanted in 60 healthy male Lewis rats as an interposition graft into the abdominal aorta and explanted at different time points up to 56 days after implantation to monitor sequential cell infiltration, differentiation, and tissue formation in the scaffold. Endogenous tissue formation started with an acute immune response, followed by a dominant presence of pro-inflammatory macrophages during the first 28 days. Next, a shift towards tissue-producing cells was observed, with a striking increase in α-Smooth Muscle Actin-positive cells and extracellular matrix by day 56. At that time, the scaffold was resorbed and immune markers were low. These results suggest that neo-tissue formation was still in progress, while the host response became quiescent, favoring a regenerative tissue outcome. Future studies should confirm long-term tissue homeostasis, but require the strengthening of the supramolecular scaffold if a non-shielded model will be used.
Mobility management and adaptive dynamic clustering for mobile wireless sensor networks (2017)

Mobile node Tracking and data gathering in wireless sensor networks (WSNs) is one of the challenging tasks. In this paper, we have shown the performance evaluation and testing results of our efficient routing algorithms in the context of MWSN for data gathering under various scenarios. In this progression, Distributed Cluster-based Algorithm for Target Tracking method is used to define the next position of the Node. Then, Prediction-Based Clustering Algorithm for Movement Detection method is used to catch the optimal data communication path. If the mobile nodes move within the range of the communicative region, it collects the data from neighbour Cluster Member (CM) and if Mobile Data Collector (MDC) moved out, it receives the routing information from neighbour cluster head (CH) and exchange that information with another MDCs. Our proposed algorithm minimizes the cluster overhead and improves the three tier routing problem using Adaptive Dynamic Clustering (ADC). Here the MDCs are performs like vehicle and gathering the information from neighbour CHs and CMs. The Performance evaluation results show that the proposed system efficiently supports for node mobility management with low control overhead and delay applications as compared to other cluster-based algorithms and also upturns the lifetime of the CH and packet delivery ratio when the number of sources Increases.
Dilepton Spectroscopy of QCD Matter at Collider Energies (2013)

Low-mass dilepton spectra as measured in high-energy heavy-ion collisions are a unique tool to obtain spectroscopic information about the strongly interacting medium produced in these reactions. Specifically, in-medium modifications of the vector spectral function, which is well known in the vacuum, can be deduced from the thermal radiation off the expanding QCD fireball. This, in particular, allows to investigate the fate of the<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>ρ</mml:mi></mml:mrow></mml:math>resonance in the dense medium and possibly infer from it signatures of the (partial) restoration of chiral symmetry, which is spontaneously broken in the QCD vacuum. After briefly reviewing calculations of thermal dilepton emission rates from hot QCD matter, utilizing effective hadronic theory, lattice QCD, or resummed perturbative QCD, we focus on applications to dilepton spectra at heavy-ion collider experiments at RHIC and LHC. This includes invariant-mass spectra at full RHIC energy with transverse-momentum dependencies and azimuthal asymmetries, as well as a systematic investigation of the excitation function down to fixed-target energies, thus making contact to previous precision measurements at the SPS. Furthermore, predictions for the energy frontier at the LHC are presented in both dielectron and dimuon channels.
The Occurrence of α(1 → 2) Linked N‐Acetylperosamine‐Homopolymer in Lipopolysaccharides of Non‐O1 Vibrio cholerae Possessing an Antigenic Factor in Common with Ol V. cholerae (1990)

Chemical analysis was carried out on lipopolysaccharides from Vibrio cholerae bio-serogroup Hakata 487-85. The O-specific chain of the phenol-soluble lipopolysaccharides was demonstrated by 13C-NMR spectroscopy and methylation analysis to contain a linear homopolymer of alpha(1----2) linked N-acetylperosamine (4-acetamido-4,6-dideoxy-D-mannopyranose), which was closely similar to but not identical to a linear alpha(1----2) linked N-3-deoxy-L-glycerotetronyl (S-2,4-dihydroxybutyryl) perosamine-homopolymer constituting that of O1 Vibrio cholerae lipopolysaccharides.
SatB2-Expressing Neurons in the Parabrachial Nucleus Encode Sweet Taste (2019)

The gustatory system plays an important role in sensing appetitive and aversive tastes for evaluating food quality. In mice, taste signals are relayed by multiple brain regions, including the parabrachial nucleus (PBN) of the pons, before reaching the gustatory cortex via the gustatory thalamus. Recent studies show that taste information at the periphery is encoded in a labeled-line manner, such that each taste modality has its own receptors and neuronal pathway. In contrast, the molecular identity of gustatory neurons in the CNS remains unknown. Here, we show that SatB2-expressing neurons in the PBN play a pivotal role in sweet taste transduction. With cell ablation, in vivo calcium imaging, and optogenetics, we reveal that SatB2<sup>PBN</sup> neurons encode positive valance and selectively transmit sweet taste signals to the gustatory thalamus.
Enantiomeric separation of ketoconazole and terconazole antifungals by electrokinetic chromatography: Rapid quantitative analysis of ketoconazole in pharmaceutical formulations (2005)

EKC using a neutral CD as chiral selector was applied in this work to the development of a method enabling the enantiomeric separation of ketoconazole and terconazole antifungals. The influence of different experimental conditions such as temperature, CD concentration, pH, and nature and concentration of the buffer on the enantiomeric resolution of the compounds studied was investigated. The use of 10 mM heptakis-(2,3,6-tri-O-methyl)-beta-CD in a 100 mM phosphate buffer (pH 3.5) with a temperature of 15 degrees C allowed the separation of the enantiomers of ketoconazole and terconazole with high resolution (R(s) > 2.0). The rapid separation of ketoconazole enantiomers with an analysis time less than 3 min was carried out after fitting some experimental parameters. The developed method was applied to the determination of ketoconazole in different pharmaceutical formulations.
One-Dimensional Ablation Using a Full Newton's Method and Finite Control Volume Procedure (2008)

The development and verification of a one-dimensional constant density material thermal response code with ablation is presented. The implicit time integrator, control volume finite element spatial discretization, and Newton's method (with an analytical Jacobian) for the entire system of residual equations have been implemented and verified for variable material properties, Q* ablation, and thermochemical ablation problems. Timing studies were performed, and when accuracy is considered, the method developed in this study exhibits significant time savings over the property lagging approach. In addition, maximizing the Newton solver's convergence rate by including sensitivities to the surface recession rate reduces the overall computational time when compared to excluding recession rate sensitivities.
Genomic Prediction of Biomass Yield in Two Selection Cycles of a Tetraploid Alfalfa Breeding Population (2015)

Alfalfa (Medicago sativa L.) is a widely planted perennial forage legume grown throughout temperate and dry subtropical regions in the world. Long breeding cycles limit genetic improvement of alfalfa, particularly for complex traits such as biomass yield. Genomic selection (GS), based on predicted breeding values obtained using genome-wide molecular markers, could enhance breeding efficiency in terms of gain per unit time and cost. In this study, we genotyped tetraploid alfalfa plants that had previously been evaluated for yield during two cycles of phenotypic selection using genotyping-by-sequencing (GBS). We then developed prediction equations using yield data from three locations. Approximately 10,000 single nucleotide polymorphism (SNP) markers were used for GS modeling. The genomic prediction accuracy of total biomass yield ranged from 0.34 to 0.51 for the Cycle 0 population and from 0.21 to 0.66 for the Cycle 1 population, depending on the location. The GS model developed using Cycle 0 as the training population in predicting total biomass yield in Cycle 1 resulted in accuracies up to 0.40. Both genotype × environment interaction and the number of harvests and years used to generate yield phenotypes had effects on prediction accuracy across generations and locations, Based on our results, the selection efficiency per unit time for GS is higher than phenotypic selection, although accuracies will likely decline across multiple selection cycles. This study provided evidence that GS can accelerate genetic gain in alfalfa for biomass yield.
Examination of the topography of the saccharide binding sites of concanavalin A and of the forces involved in complexation (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExamination of the topography of the saccharide binding sites of concanavalin A and of the forces involved in complexationRonald D. Poretz and Irwin J. GoldsteinCite this: Biochemistry 1970, 9, 14, 2890–2896Publication Date (Print):July 7, 1970Publication History Published online1 May 2002Published inissue 7 July 1970https://pubs.acs.org/doi/10.1021/bi00816a021https://doi.org/10.1021/bi00816a021research-articleACS PublicationsRequest reuse permissionsArticle Views237Altmetric-Citations204LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Outcomes Following Erosions of the Artificial Urinary Sphincter (2006)

Our study suggests that patients with comorbidities including hypertension, coronary artery disease, prior radiation therapy and prior AUS revisions are more likely to have erosions of their AUS. Nevertheless, continence can still be salvaged using various strategies which optimize use of the remaining healthy urethral tissue.
Effects of Oven and Laser Sintering Parameters on the Electrical Resistance of IJP Nano-Silver Traces on Mesoporous PET Before and During Fatigue Cycling (2019)

Inkjet printing of conducting traces offers well established advantages and disadvantages as an alternative to electroplating of interconnects in flexible electronics. Assessment and optimization of their reliability is, however, often more complicated than commonly recognized. This is the case for an approach based on the deposition of silver nano-particle inks onto mesoporous PET substrates. In this case heating leads the trace resistance to drop not only because of the shrinkage and cure of the organic matrix holding the particles together, but also because some of that matrix 'disappears' into the substrate pores. The substrates can however only sustain relatively brief excursions above their glass transition, nominally 75°C, so it is not always practical to sinter the traces completely by conventional means. That has consequences such as ongoing reductions in resistance over time or under cyclic loading. Laser sintering does however offer the opportunity for much better fusing of the particles without excessive heating of the PET. The present work addresses effects of sintering parameters such as time/temperature and power/speed in oven and laser sintering, respectively, on the initial resistance and its evolution in subsequent low cycle fatigue testing. Interconnects of an average width of 80 μm and thickness of 550 nm were printed and post processed by one of two different sintering techniques: a) Convection oven sintering, and (b) Laser sintering. The resulting resistances were quantified, and samples finally subjected to tensile cycling with amplitudes of 1-2% and in-situ monitoring of the resulting resistance changes using a four-point probe. As expected, the resistance increased in each cycle as the substrate was stretched and it decreased again during unloading. However unlike for other kinds of traces, even though a remaining viscoelastic strain on the substrate prevented the complete elimination of the strain on the trace, the resistance of oven sintered traces usually ended up slightly lower after each cycle than before it. This effect was stronger for higher strain amplitudes, but it could be reduced or eliminated by longer preceding sintering of the traces. While a reduction in resistance may seem preferable to an increase, an even better solution would be a lower initial resistance that remained insensitive to subsequent fatigue cycling. This could be achieved by laser sintering, but careful optimization was required as too low a power did not prevent further resistance drops in cycling while too high ones led to significant degradations in fatigue resistance.
Feedback charge-transfer comparator with zero static power (2003)

The feedback charge transfer (FCT) amplifier latch comparator uses a dynamic feedback mechanism in the charge transfer (CT) preamplifier. The FCT amplifier latch rapidly amplifies input signal and latches with zero static power. Circuit operation is nearly insensitive to device parameter fluctuations. The authors present the design and performance characteristics of such a comparator implemented in a 1.2 /spl mu/m double-poly double metal CMOS process.
Nanoscale resistive memory with intrinsic diode characteristics and long endurance (2010)

We report studies on nanoscale resistive memory devices that exhibit diodelike I-V characteristics at on-state with reverse bias current suppressed to below 10−13 A and rectifying ratio &amp;gt;106. The intrinsic diodelike characteristics are robust during device operation and can survive &amp;gt;108 write/erase programming cycles. The devices can be programmed at reduced programming voltages compared to earlier studies without the initial high-voltage forming process. Multibit storage capability was also reported. The intrinsic diode characteristics provide a possible solution to suppress crosstalk in high-density crossbar memory or logic arrays particularly for those based on bipolar resistive switches (memristors).
Human health effects and Pfiesteria exposure: a synthesis of available clinical data. (2001)

An association between human illness and exposure to Pfiesteria was first observed among laboratory personnel working with the microorganism. In 1997, in the setting of Pfiesteria activity on the Pocomoke River in Maryland, difficulties with learning and memory were epidemiologically associated with high-level exposure to waterways in which the organism was known to be present. In the Maryland studies, neurocognitive function of affected persons returned to within normal ranges within a period of 3-6 months. Persons with the most severe neurocognitive deficits were significantly more likely to have skin lesions characterized on biopsy by evidence of a toxic/allergic inflammatory reaction. Acute high-level exposures to waterways where Pfiesteria has been identified have been linked with eye and respiratory irritation, headache, and gastrointestinal complaints. Recent data, collected using molecular techniques, suggest that the organism is present in multiple locations in the Chesapeake Bay environment; available data are insufficient to comment on the possible cumulative health impact of chronic low-level environmental exposure to Pfiesteria.
Superconducting Y-Ba-Cu-O Oxide Films by OMCVD (1988)

Formation of Y-Ba-Cu-O films have been attempted by the organometallic chemical vapor deposition (OMCVD) method using Y(thd) 3 , Ba(thd) 2 , and Cu(thd) 2 (thd=2,2,6,6,-tetramethyl-3,5-heptanedione), which are organic metal chelates, as gas sources. The results of electron probe microanalysis (EPMA) show that the film formed has a composition similar to that of YBa 2 Cu 3 O x ceramics. The film has a high resistivity in the as-deposited state. If the film is conditioned at 950°C for 30 minutes, the resistivity reduces extremely, providing a superconductivity. T c onset is 76 K and T c final is 60 K.