gebhart/patrepeval huggingface dataset:
Site-specific O-glycosylation of members of the low-density lipoprotein receptor superfamily enhances ligand interactions (2018)

The low-density lipoprotein receptor (LDLR) and related receptors are important for the transport of diverse biomolecules across cell membranes and barriers. Their functions are especially relevant for cholesterol homeostasis and diseases, including neurodegenerative and kidney disorders. Members of the LDLR-related protein family share LDLR class A (LA) repeats providing binding properties for lipoproteins and other biomolecules. We previously demonstrated that short linker regions between these LA repeats contain conserved <i>O</i>-glycan sites. Moreover, we found that <i>O</i>-glycan modifications at these sites are selectively controlled by the GalNAc-transferase isoform, GalNAc-T11. However, the effects of GalNAc-T11-mediated <i>O</i>-glycosylation on LDLR and related receptor localization and function are unknown. Here, we characterized <i>O</i>-glycosylation of LDLR-related proteins and identified conserved <i>O</i>-glycosylation sites in the LA linker regions of VLDLR, LRP1, and LRP2 (Megalin) from both cell lines and rat organs. Using a panel of gene-edited isogenic cell line models, we demonstrate that GalNAc-T11-mediated LDLR and VLDLR <i>O</i>-glycosylation is not required for transport and cell-surface expression and stability of these receptors but markedly enhances LDL and VLDL binding and uptake. Direct ELISA-based binding assays with truncated LDLR constructs revealed that <i>O</i>-glycosylation increased affinity for LDL by ∼5-fold. The molecular basis for this observation is currently unknown, but these findings open up new avenues for exploring the roles of LDLR-related proteins in disease.
3D reconstruction of human retina from a single fundus image (2011)

Abstract- A major imaging technique for the eye is the fundus camera, and images obtained from this camera are 2D in nature. 3D retinal image would be helpful in explaining to patients, about the progression of a disease in the eye, and for diagnostic records. This work focusses on the 3D reconstruction of retinal image from a single fundus image. The reconstruction is achieved using the shape from shading (SFS) method, and the focussing information. The results obtained are fascinating and are presented.
Recent Performance of Nonpolar/Semipolar GaN-based Blue LEDs/LDs (2007)

Violet InGaN/GaN light emitting diodes (LEDs) was fabricated on semipolar GaN bulk substrates. The output power and external quantum efficiency at a driving current of 20 mA were measured. The first nonpolar m-plane nitride laser diodes (LDs) were realized on low extended defect bulk m-plane GaN substrates.
Genital Cysts of the Male Pelvis: Case Report of Müllerian and Ejaculatory Duct Cysts in the Same Patient (1973)

No AccessJournal of Urology1 Mar 1973Genital Cysts of the Male Pelvis: Case Report of Müllerian and Ejaculatory Duct Cysts in the Same Patient Donald T. Lucey, Jack W. McAninch, and R.C. Bunts Donald T. LuceyDonald T. Lucey Current address: Medical Arts Building, 1110 Wake Forest Road, Raleigh, North Carolina 27604. More articles by this author , Jack W. McAninchJack W. McAninch More articles by this author , and R.C. BuntsR.C. Bunts More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)60447-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1973 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByDESAUTEL M, STOCK J and HANNA M (2018) MULLERIAN DUCT REMNANTS: SURGICAL MANAGEMENT AND FERTILITY ISSUESJournal of Urology, VOL. 162, NO. 3 Part 2, (1008-1013), Online publication date: 1-Sep-1999.Mayersak J (2018) Urogenital Sinus-Ejaculatory Duct Cyst: A Case Report with a Proposed Clinical Classification and Review of the LiteratureJournal of Urology, VOL. 142, NO. 5, (1330-1332), Online publication date: 1-Nov-1989.Ritchey M, Benson R, Kramer S and Kelalis P (2018) Management of Müllerian Duct Remnants in the Male PatientJournal of Urology, VOL. 140, NO. 4, (795-799), Online publication date: 1-Oct-1988.Takatera H, Sugao H and Sakurai T (2018) Ejaculatory Duct Cyst: The Case for Effective Use of Transrectal Longitudinal UltrasonographyJournal of Urology, VOL. 137, NO. 6, (1241-1242), Online publication date: 1-Jun-1987.Elder J and Mostwin J (2018) Cyst of the Ejaculatory Duct/Urogenital SinusJournal of Urology, VOL. 132, NO. 4, (768-771), Online publication date: 1-Oct-1984. Volume 109Issue 3March 1973Page: 440-443 Advertisement Copyright & Permissions© 1973 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Donald T. Lucey Current address: Medical Arts Building, 1110 Wake Forest Road, Raleigh, North Carolina 27604. More articles by this author Jack W. McAninch More articles by this author R.C. Bunts More articles by this author Expand All Advertisement PDF DownloadLoading ...
Human Serum Albumin Incorporating Tetrakis(o-pivalamido) phenylporphinatoiron(II) Derivative as a Totally Synthetic O2-Carrying Hemoprotein (1999)

2-[8-{N-(2-Methylimidazolyl)}octanoyloxymethyl]-5,10,15, 20-tetrakis(o-pivalamido)phenylporphinatoiron(II)s (FePs) were incorporated into hydrophobic cavities of recombinant human serum albumin (rHSA), providing a totally synthetic O(2)-carrying hemoprotein (rHSA-FeP). An rHSA host absorbs maximally eight FeP molecules. Solution properties of the obtained albumin hybrid [[rHSA] = 5 wt %; FeP/HSA = 1-8 (mol/mol)] are almost identical to those of the rHSA itself; the specific gravity is 1.013 and the viscosity is 1.1 cP. Circular dichroism spectroscopy and isoelectric focusing measurement revealed that the second-order structure and surface charge distribution of rHSA were always constant independent of the binding numbers of FeP. Hydrophobic interaction is probably a major molecular force of the incorporation of this synthetic heme. rHSA-FeP can bind and release dioxygen reversibly under physiological conditions (in aqueous media, pH 7.3, 37 degrees C) like hemoglobin and myoglobin. Its O(2)-coordination structure was evaluated by resonance Raman spectroscopy. The O(2) rebinding after the laser flash photolysis showed three-phases decay, which were analyzed by triple-exponential kinetics. The O(2)-binding affinity and O(2)-association and -dissociation rate constants of rHSA-FeP satisfy the initial clinical requirements for O(2) infusion as a red cell substitute.
Steel bonded dense silicon nitride compositions and method for their fabrication (2023)

A two-stage bonding technique for bonding high density silicon nitride and other ceramic materials to stainless steel and other hard metals, and multilayered ceramic-metal composites prepared by the technique are disclosed. The technique involves initially slurry coating a surface of the ceramic material at about 1500.degree. C. in a vacuum with a refractory material and the stainless steel is then pressure bonded to the metallic coated surface by brazing it with nickel-copper-silver or nickel-copper-manganese alloys at a temperature in the range of about 850.degree. to 950.degree. C. in a vacuum. The two-stage bonding technique minimizes the temperature-expansion mismatch between the dissimilar materials.
Hydrogen‐Borrowing and Interrupted‐Hydrogen‐Borrowing Reactions of Ketones and Methanol Catalyzed by Iridium (2014)

Abstract Reported herein is the use of catalytic [{Ir(cod)Cl} 2 ] to facilitate hydrogen‐borrowing reactions of ketone enolates with methanol at 65 °C. An oxygen atmosphere accelerates the process, and when combined with the use of a bulky monodentate phosphine ligand, interrupts the catalytic cycle by preventing enone reduction. Subsequent addition of pro‐nucleophiles to the reaction mixture allowed a one‐pot methylenation/conjugate addition protocol to be developed, which greatly expands the range of products that can be made by this methodology.
RNA SYNTHESIS IN CHINESE HAMSTER CELLS (1968)

The incorporation of methionine-methyl-(14)C into 18S ribosomal RNA of cultured Chinese hamster ovary cells in early and late interphase has been determined by zone-sedimentation analysis of phenol-extracted RNA preparations. Synchronized cell cultures were prepared for these studies by thymidine treatment and by mechanical selection of mitotic cells. The specific activity of 18S RNA labeled in late interphase was found to be 1.1-1.2 times that of 18S RNA labeled in early interphase. Upon correction for increase in RNA mass, the rate of methylation of 18S RNA in late interphase is about 1.9 times that in early interphase.
Ni(Pt)Si thin film formation and its electrical characteristics with Si substrate (2002)

The solid phase silicidations for the bilayers of Ni/Pt and Pt/Ni sputtered on Si[100] substrates were studied. The effect of Pt addition in the NiSi film on its thermal stability enhancement was investigated. The results show that the phase transformation from Ni(Pt)Si to NiSi/sub 2/ was delayed to higher temperature than the Ni/Si system without Pt as a capping- or inter-layer by more than 100/spl deg/C. The apparent Schottky barrier height of Ni(Pt)Si/n-Si(111) is modulated with the Pt content, and the electrical characteristic is good in the silicidation temperature of 500-800/spl deg/C.
Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium (2015)

Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu(24) and Trp(87) to yield the mature (circular) enterocin NKR-5-3B.
Molecular research and genetic engineering of resistance to Verticillium wilt in cotton: A review (2009)

Verticillium dahliae, a soil-borne pathogen, causes Verticillium wilt, one of the most serious diseases in cotton, deleteriously influencing crop’s production and quality. Verticillium wilt has become a major obstacle in cotton production since Helicoverpa armigera, the cotton bollworm, became effectively controlled in recent years. The wilt is becoming a key subject of research in cotton-resistance genetics, breeding and plant pathology. This paper reviews the recent research progress on genetic methods of resistance, the status and existing problems, traditional breeding, the main resistance mechanism, molecular markers and genetic engineering of resistance genes. It is hoped that new breeding methods and new varieties resistant to Verticillium wilt will be developed in the very near future.
Erratum to: Measurement of the CKM angle γ using B± → DK± with D → K S 0π+π−, K S 0K+K− decays (2018)

LPHE
Magnetic steering control of multi-cellular bio-hybrid microswimmers (2014)

Bio-hybrid devices, which integrate biological cells with synthetic components, have opened a new path in miniaturized systems with the potential to provide actuation and control for systems down to a few microns in size. Here, we address the challenge of remotely controlling bio-hybrid microswimmers propelled by multiple bacterial cells. These devices have been proposed as a viable method for targeted drug delivery but have also been shown to exhibit stochastic motion. We demonstrate a method of remote magnetic control that significantly reduces the stochasticity of the motion, enabling steering control. The demonstrated microswimmers consist of multiple Serratia marcescens (S. marcescens) bacteria attached to a 6 μm-diameter superparamagnetic bead. We characterize their motion and define the parameters governing their controllability. We show that the microswimmers can be controlled along two-dimensional (2-D) trajectories using weak magnetic fields (≤10 mT) and can achieve 2-D swimming speeds up to 7.3 μm s(-1). This magnetic steering approach can be integrated with sensory-based steering in future work, enabling new control strategies for bio-hybrid microsystems.
KCR1, a Membrane Protein That Facilitates Functional Expression of Non-inactivating K+ Currents Associates with Rat EAG Voltage-dependent K+Channels (1998)

Cerebellar granule neurons possess a non-inactivating K+ current, which controls resting membrane potentials and modulates the firing rate by means of muscarinic agonists. kcr1 was cloned from the cerebellar cDNA library by suppression cloning. KCR1 is a novel protein with 12 putative transmembrane domains and enhances the functional expression of the cerebellar non-inactivating K+ current in Xenopus oocytes. KCR1 also accelerates the activation of rat EAG K+ channels expressed in Xenopus oocytes or in COS-7 cells. Far-Western blotting revealed that KCR1 and EAG proteins interacted with each other by means of their C-terminal regions. These results suggest that KCR1 is the regulatory component of non-inactivating K+ channels.
Properties of the Neosquamous Epithelium After Radiofrequency Ablation of Barrett's Esophagus Containing Neoplasia (2009)

Rigorous evaluation of the post-RFA NSE in patients who, at baseline, had BE containing early cancer high-grade intraepithelial neoplasia, showed neither persistent genetic abnormalities nor buried glandular mucosa.
Quantitative Measurement of Local Cerebral Blood Flow in the Anesthetized Mouse Using Intraperitoneal [14C]Iodoantipyrine Injection and Final Arterial Heart Blood Sampling (2000)

Autoradiographic measurement of local cerebral blood flow (CBF) with [14C]iodoantipyrine (IAP) is limited in mice by the difficulty in cannulating vessels and the blood loss for repeated blood sampling. The authors modified and validated the method to measure local CBF with [14C]IAP in mice by combining intraperitoneal tracer application with a single blood sampling from the heart at the end of the experiment. Experiments were carried out in male SV129 mice under halothane anesthesia. After intraperitoneal administration of 15 microCi [14C]IAP, arterial blood samples were collected repeatedly and anesthetized animals were immersed in liquid nitrogen. In addition, frozen blood from the heart was sampled to obtain the final blood [14C]radioactivity. Correlation analysis between the sampling time and [14C]radioactivity of the arterial blood revealed a highly significant linear relationship (P < 0.001, r = 0.978) and a lag time of the [14C]tracer in arterial blood of 3.3 +/- 0.6 seconds. [14C]radioactivity of the final arterial blood sample (444 +/- 264 nCi/mL) was almost equal to that of the heart blood (454 +/- 242 nCi/mL), and the absolute difference in each animal was 3.3 +/- 4.2% (mean +/- SD). The convolution integrals for the CBF calculation were determined either by integrating the radioactivity of individual arterial blood samples or by assuming a linear rise from [14C]tracer lag time after intraperitoneal [14C]IAP injection to the value measured in the blood sample from the frozen heart. Regional flow values calculated by the two methods differed by less than 11% (not significant). This method allows the quantitative measurement of local CBF in anesthetized mice without any vessel catheterization and will make mutant mice a more powerful tool to elucidate the molecular mechanisms of brain injuries by combining flow studies with molecular-biological methods.
Efficient transformation of chicken embryo fibroblasts by c-Jun requires structural modification in coding and noncoding sequences. (1990)

To assess the transforming capability of the c-Jun protein, we introduced the chicken c-jun proto-oncogene into a replication competent avian retroviral expression vector (RCAS). Viral Jun efficiently transformed chicken embryo fibroblasts (CEFs) when expressed from this vector. Overexpression of c-Jun leads to transformation of CEFs with an efficiency that is 15- to 25-fold less than that seen for v-Jun, suggesting that v-Jun contains structural features that increase its oncogenic potential relative to c-Jun. There are four structural differences between v-Jun and c-Jun. To determine the relative contribution that each of these structural differences between v-Jun and c-Jun has on oncogenic activity, several deletion and substitution mutants were constructed. Each of these mutants was expressed in CEF and assayed for transformation by focus formation. Analysis of the results reveals that deletion of a region of 27 amino acids near the amino terminus of c-Jun and deletion of 3'-untranslated sequences are critical in activating the full oncogenic potential of Jun.
Miscibility between Differently Shaped Mesogens: Structural and Morphological Study of a Phthalocyanine-Perylene Binary System (2009)

The thermotropic, structural, and morphological properties of blends of a disk-like liquid crystalline phthalocyanine derivative and a lath-shaped perylenetetracarboxidiimide mesogen derivative have been studied by combining differential scanning calorimetry, thermal polarized optical microscopy, X-ray diffraction, solid-state nuclear magnetic resonance, and atomic force microscopy. The two compounds are fully miscible for blends containing at least 60 mol % of the disk-like molecule. In such composition range, the homogeneous blends form a columnar hexagonal (Col(h)) mesophase for which the thermal stability is enhanced compared to that of the corresponding mesophase of the pure phthalocyanine. The miscible blends self-align homeotropically between two glass slides. For blends containing between 55 and 40 mol % of the disk-shaped molecule, the two components are fully miscible at high temperature but the perylene derivative forms a separate crystalline phase when the temperature is decreased. Phase separation is systematically observed in blends containing less than 40 mol % of the discotic molecule. In this case, the resulting Col(h) mesophase is less stabilized compared to the blends containing a larger amount of the phthalocyanine derivative. These phase-separated blends do not show any homeotropic alignment. AFM investigations confirm the formation of a single columnar morphology in the phthalocyanine-rich blends, consistent with the full miscibility between the two compounds. Solid-state NMR measurements on the mixed phase show the influence of the presence of the perylene molecules on the molecular dynamics of the molecules; remarkably, the presence of the host molecules improves the local order parameter in the phthalocyanine columnar phase.
Assessment of Evidence Base from Medical Debriefs Data on Space Motion Sickness Incidence and Treatment (2008)

An objective of this data compilation and analysis project is to examine incidence and treatment efficacy of common patho-physiological disturbances during spaceflight. Analysis of medical debriefs data indicated that astronauts used medications to alleviate symptoms of four major ailments for which astronauts received treatment for sleep disturbances, space motion sickness (SMS), pain (headache, back pain) and sinus congestion. In the present data compilation and analysis project on SMS treatment during space missions, subject demographics (gender, age, first-time or repeat flyer), incidence and severity of SMS symptoms and subjective treatment efficacy from 317 crewmember debrief records were examined from STS-1 through STS-89. Preliminary analysis of data revealed that 50% of crew members reported SMS symptoms on at least one flight and 22% never experienced it. In addition, there were 387 medication dosing episodes reported, and promethazine was the most commonly used medication. Results of analysis of symptom check lists, medication use/efficacy and gender and flight record differences in incidence and treatment efficacy will be presented. Evidence gaps for treatment efficacy along with medication use trend analysis will be identified.
Supplementary Figure 1 from Quiescin Sulfhydryl Oxidase 1 Promotes Invasion of Pancreatic Tumor Cells Mediated by Matrix Metalloproteinases (2023)

&lt;p&gt;PDF file - 187K, Tyrpan Blue Assay.&lt;/p&gt;
Comparing the immune response to a novel intranasal nanoparticle PLGA vaccine and a commercial BPI3V vaccine in dairy calves (2015)

Calves administered the intranasal nanoparticle vaccine induced significantly greater mucosal IgA responses, compared to the other treatment groups. This suggests an enhanced, sustained mucosal-based immunological response to the BPI3V nanoparticle vaccine in the face of pre-existing antibodies to BPI3V, which are encouraging and potentially useful characteristics of a candidate vaccine. However, ability of nanoparticle vaccine in eliciting cell mediated immune response needs further investigation. More sustained local mucosal immunity induced by nanoparticle vaccine has obvious potential if it translates into enhanced protective immunity in the face of virus outbreak.
Where is biological nutrient removal going now? (2006)

With more than 30 years of experience multiple options exist for removal of nitrogen and phosphorus from wastewater. Communities that were exempt from nutrient removal for many years must now comply with imposed nutrient limits, and in areas where technology-based nutrient limits have been in place communities are now faced with more stringent mass-based limits that are becoming more difficult to meet as their populations increase. Recent efforts in the industry have been focused on getting more out of existing plants, or in many cases where land is not available, in intensifying existing processes to increase capacity and/or level of treatment. This paper will discuss some of these methods and the general direction in which biological nutrient removal is developing to address these new challenges.
Therapy and clinical trials (2005)

aDivision of Endocrinology and Metabolism, UT Southwestern Medical Center, Dallas, Texas, USA bLaboratory for Atherosclerosis and Metabolic Research, University of California, Davis Medical Center, Sacramento, California, USA Correspondence to Ishwarlal Jialal, Laboratory for Atherosclerosis and Metabolic Research, UC Davis Medical Center, 4635 2nd Avenue, Research 1 Building, Room 3000, Sacramento, CA 95817, USA Tel: +1 916 734 6590; fax: +1 916 734 6593; e-mail: [email protected] Sponsorship: This work was supported by grant K-2400596 from the National Institutes of Health, Bethesda, Maryland, USA.
Isotretinoin and Recombinant Interf eron Alfa-2a Therapy of Metastatic Malignant Melanoma (1996)

Twenty-five patients with metastatic malignant melanoma were treated with isotretinoin (13-cis-retinoic acid) orally at 1 mg/kg daily and recombinant interferon alfa-2a (INF-alpha) subcutaneously at 3 million units daily for 16-48 weeks. Therapy was well tolerated; fatigue and hyperlipidemia were the most frequent dose-limiting toxicity and necessitated dose reductions in 14 patients. Two patients achieved a complete response, and 3 responded partially for a total response rate of 20% (95% confidence interval: 4-36%). Responses occurred primarily in patients with limited tumor burden and disease confined to the skin and lymph nodes. Significant elevations in peripheral blood 2'-5'-oligoadenylate synthetase activity and natural killer activity were observed with therapy. The magnitude of these changes, however, was not predictive of response. Biopsy specimens of two responding lesions showed extensive necrosis of tumor. One specimen showed large aggregates of melanophages in association with tumor. The combination of isotretinoin and IFN-alpha is an active, easily administered regimen with acceptable toxicity for metastatic malignant melanoma.