gebhart/patrepeval huggingface dataset:
A colorimetric probe based on diketopyrrolopyrrole and tert-butyl cyanoacetate for cyanide detection (2015)

A new colorimetric probe <bold>1</bold> comprising a diketopyrrolopyrrole-based Michael receptor, which recognized cyanide anions with high selectivity, was designed and synthesized.
Recognition of Sequence-Directed DNA Structure by the Klenow Fragment of DNA Polymerase I (1998)

Time-resolved fluorescence spectroscopy was used to investigate the influence of sequence-directed DNA structure upon the interaction between the Klenow fragment of DNA polymerase I and a series of defined oligonucleotide primer/templates. 17/27-mer (primer/template) oligonucleotides containing a dansyl fluorophore conjugated to a modified deoxyuridine residue within the primer strand were used as substrates for binding to Klenow fragment. The time-resolved fluorescence anisotropy decay of the dansyl probe was analyzed in terms of two local environments, either solvent-exposed or buried, corresponding to primer/templates positioned with the primer 3' terminus in the polymerase site or the 3'-5' exonuclease site of the enzyme, respectively. Equilibrium constants for partitioning of DNA between the two sites were evaluated from the anisotropy decay data for primer/templates having different (A + T)-rich sequences flanking the primer 3' terminus. Primer/templates with AAAATG/TTTTAC and CGATAT/GCTATA terminal sequences (the nucleotides on the left refer to the last six bases at the 3' end of the primer, and the nucleotides on the right are the corresponding bases in the template) were bound mostly at the polymerase site. The introduction of single mismatches opposite the primer 3' terminus of these DNA substrates increased their partitioning into the 3'-5' exonuclease site, in accord with the results of an earlier study [Carver, T.E., Hochstrasser, R.A., and Millar, D.P. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 10670-10674]. In contrast, a primer/template with the terminal sequence CAATTT/GTTAAA, containing an A-tract element AATTT, exhibited a surprising preference for binding at the 3'-5' exonuclease site, despite the absence of mismatched bases in the DNA substrate. Interruption of the A-tract with a single AG step, to give the terminal sequence CAGTTT/GTCAAA, reversed the effect of the A-tract, causing the DNA to partition in favor of the polymerase site. Moreover, the presence of a single mismatch opposite the primer 3' terminus was also sufficient to reverse the effect of the A-tract, resulting in a distribution of DNA between polymerase and 3'-5' exonuclease sites that was similar to that observed for the other mismatched DNA substrates. Taken together, these results suggest that the A-tract adopts an unusual conformation that is disruptive to binding at the polymerase site. The effect of the A-tract on binding of DNA to the polymerase site is discussed in terms of the unusual helix structural parameters associated with these sequence elements and the difference between the local geometry of the A-tract and the conformation adopted by duplex DNA within the polymerase cleft. The results of this study show that in addition to base mismatches, Klenow fragment can also recognize irregularities in the helix geometry of perfectly base-paired DNA.
Tárgyilagos határvonal (2022)

A hazai -s a magyar kutatk klfldi tjainak ksznheten nemzetkzi -nprajzi
Supplementary Tables S1-S2 from SLC46A3 Is Required to Transport Catabolites of Noncleavable Antibody Maytansine Conjugates from the Lysosome to the Cytoplasm (2023)

&lt;p&gt;Genes identified as critical for CD70 ADC function in Library 1 screen (S1); Genes identified as critical for CD70 ADC function in Library 2 screen (S2).&lt;/p&gt;
Abstract PR07: Integrative functional genomics of breast cancer (2013)

Abstract Cancer is a disease of the genome. Using genomic approaches alone, it is difficult to ascertain which variants drive pathogenesis because most of these are functionally irrelevant passenger mutations. Highly recurrent events point to drivers; however, the majority of genomic alterations in tumors occur at low frequency. In addition, the products of many oncogenes and tumor suppressor genes are not druggable. However, such abnormalities can cause unanticipated gene/pathway dependencies (synthetic lethality), providing alternate avenues for drug development. Lentiviral-based shRNA libraries enables genome-wide screening of cultured cancer cells in a pooled format, facilitating the identification of genes necessary for cancer cell proliferation and survival in cultured cells. We screened a panel of &amp;gt; 75 breast cancer cell lines using an 80,000 lentiviral shRNA library targeting 16,000 genes and integrated these screen results with gene expression, copy-number variation (CNV), and somatic mutations derived from the same lines. We identified several classes of gene dropouts, which are required for survival or growth of all (or some) cell lines, irrespective of subtype and several subtype-specific genes, whose essentiality is restricted to a defined subtype. These include well-known HER2 subtype-specific genes, ERBB2, ERBB3, and TFAP2C and luminal subtype-specific gene FOXA1, SPDEF, GATA3, and ESR1. In addition, the unprecedented number of lines allows the identification of synthetic lethal interaction with common breast cancer somatic mutations or CNV such as PIK3CA and PTEN or 9p21 deletion, respectively, which encodes the CDKN2A, CDKN2B, and MTAP genes. Finally, integration of gene expression, copy number variation, and functional screening results identified potential biomarkers with common genetic changes and functional drivers. Overall, our study represents an extensive functional genetic survey of breast cancer, reveals complexities between genomic and functional genomic results, and uncovers several unexpected gene dependencies and potential novel therapeutic target for each subtype. This abstract is also presented as Poster A033. Citation Format: Richard Marcotte, Azin Sayad, Cathy Iorio, Maliha Haider, Jason Moffat, Benjamin G. Neel. Integrative functional genomics of breast cancer. [abstract]. In: Proceedings of the AACR Special Conference on Advances in Breast Cancer Research: Genetics, Biology, and Clinical Applications; Oct 3-6, 2013; San Diego, CA. Philadelphia (PA): AACR; Mol Cancer Res 2013;11(10 Suppl):Abstract nr PR07.
Chemistry of singlet oxygen. VII. Quenching by .beta.-carotene (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of singlet oxygen. VII. Quenching by .beta.-caroteneChristopher S. Foote and Robert W. DennyCite this: J. Am. Chem. Soc. 1968, 90, 22, 6233–6235Publication Date (Print):October 1, 1968Publication History Published online1 May 2002Published inissue 1 October 1968https://pubs.acs.org/doi/10.1021/ja01024a061https://doi.org/10.1021/ja01024a061research-articleACS PublicationsRequest reuse permissionsArticle Views1964Altmetric-Citations554LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Systematic Review of the Measurement Properties of Tools Used to Measure Behaviour Problems in Young Children with Autism (2015)

CRD42012002223.
Control of somite patterning by Sonic hedgehog and its downstream signal response genes (1998)

In the avian embryo, previous work has demonstrated that the notochord provides inductive signals to activate myoD and pax1 regulatory genes, which are expressed in the dorsal and ventral somite cells that give rise to myotomal and sclerotomal lineages. Here, we present bead implantation and antisense inhibition experiments that show that Sonic hedgehog is both a sufficient and essential notochord signal molecule for myoD and pax1 activation in somites. Furthermore, we show that genes of the Sonic hedgehog signal response pathway, specifically patched, the Sonic hedgehog receptor, and gli and gli2/4, zinc-finger transcription factors, are activated in coordination with somite formation, establishing that Sonic hedgehog response genes play a regulatory role in coordinating the response of somites to the constitutive notochord Sonic hedgehog signal. Furthermore, the expression of patched, gli and gli2/4 is differentially patterned in the somite, providing mechanisms for differentially transducing the Sonic hedgehog signal to the myotomal and sclerotomal lineages. Finally, we show that the activation of gli2/4 is controlled by the process of somite formation and signals from the surface ectoderm, whereas upregulation of patched and activation of gli is controlled by the process of somite formation and a Sonic hedgehog signal. The Sonic hedgehog signal response genes, therefore, have important functions in regulating the initiation of the Sonic hedgehog response in newly forming somites and in regulating the patterned expression of myoD and pax1 in the myotomal and sclerotomal lineages following somite formation.
Determination of serum and urinary aluminum by HPLC with fluorometric detection of Al-lumogallion complex (1996)

We describe a simple and sensitive HPLC method for quantifying aluminum (Al) in biological fluids by measuring the fluorescence of the Al-lumogallion complex (excitation wavelength 500 nm, emission wavelength 575 nm). Serum samples are deproteinized with 0.83 mol/L perchloric acid and centrifuged; the supernates are mixed with lumogallion reagent. Urine samples are pretreated with sodium hydroxide (2 mol/L) and methanol, kept for 1 h at -20 degrees C, and then centrifuged; the precipitate is resuspended in perchloric acid and mixed with lumogallion reagent, as for serum. The maximal fluorescence complex is formed after 1 h at pH 5 +/- 0.5. The HPLC mobile phase consists of (per liter) 100 mL, of 0.2 mol/L potassium hydrogen phthalate, 220 mL of acetonitrile, and distilled deionized water. The flow rate is 1 mL/min, and the injection volume is 5 microliters. The major aluminum species is eluted at 3.5 min, the lowest detection limit being 0.45 pg. We validated the method with samples collected from normal subjects and from workers occupationally exposed to aluminum. Comparing the results with those by traditional atomic absorption spectrometry of urinary aluminum suggests that the proposed method is reliable.
Histological and Ultrastructural Evaluation of the Effects of a Radiofrequency-Based Nonablative Dermal Remodeling Device (2004)

Our findings suggest that collagen fibril contraction occurs immediately after treatment and gives rise to tissue contraction and thermally mediated wounding, which induces new collagen production.
Molecular Mechanisms of Chromosome Translocation in Human B‐ and T‐cell Neoplasiaa (1987)

Our understanding of the molecular biology of many tumors is still rudimentary. The genes involved in most solid tumors, and the mechanisms giving rise to their activation, are virtually unknown. In contrast, for hematopoietic malignancies, we have identified several genes important in oncogenesis, and we understand in at least a limited sense the mechanisms by which translocations impart these genes with the capacity to support malignant growth. It is becoming increasingly apparent that these mechanisms involve very subtle changes in the pattern of gene expression. Similarly, the studies on the mechanisms of chromosome translocation described here underscore the proposition that malignancy may arise by slight perturbations of normal function. The enzymes that physiologically recombine Ig and TCR genes do so with high fidelity under normal circumstances. But occasionally misrecognition of target sequences may lead to chromosome translocation and neoplasia. A deeper understanding of this process will be facilitated by a more certain grasp of normal B- and T-lymphocyte differentiation and proliferation.
Output power stabilization of a XeCl excimer laser by HCl gas injection (1991)

A laser output power control system which has an ability to realize high stability of the laser output power in long-term operation is proposed for high-power rare-gas halide excimer lasers. The system has a function of maintaining laser gas conditions, mainly the halogen gas concentration, constant against the gas degradation in the laser tube. As a result the laser output power is stabilized at near maximum available power. The system has been applied to a 20-W UV-preionozed XeCl excimer laser and the laser output power has been stabilized for 8 hours successfully with a fluctuation less than +/- 2% at 20 W.
Genoprotective Activity of Edible and Medicinal Mushroom Components (2004)

Hot (100 °C) and cold (20 °C) water extracts of the fruit bodies of selected mushroom species have been tested for their ability to prevent H2O2-induced oxidative damage to cellular DNA using the single-cell gel electrophoresis ("Comet") assay. Highest genoprotection was observed with cold water and hot water extracts of Agaricus bisporus (J.Lge) Imbach and Ganoderma lucidum (W. Curt.:Fr.) Lloyd fruit bodies, respectively. The genoprotective effect of A. bisporus is associated with a heat-labile protein that has been purified following salt fractionation, combined with ion-exchange, hydrophobic interaction, and adsorption chromatography. Based on catalytic and electrophoretic properties and enzyme inhibition studies, the protein was identified as tyrosinase. The genoprotective effect of the protein is dependent upon the enzyme-catalyzed conversion of tyrosine to L-DOPA and subsequent conversion of this metabolite to tyrosinase-generated L-DOPA oxidation products such as dopaquinone. We propose that these oxidation products trigger cellular processes that upregulate the overall antioxidant status of the cell and could be incorporated into preventive strategies and treatments of pathological conditions associated with elevated oxidative DNA damage and other manifestations of increased oxidative stress.
Platelet Activation and Coronary Stent Implantation (1996)

Significant platelet activation occurs in patients receiving anticoagulation therapy after stenting, while platelet deactivation is found in patients treated with combined antiplatelet therapy. This may contribute to a lowering of the incidence of subacute stent thrombosis.
Supporting distributed shared memory on multi-core network-on-chips using a dual microcoded controller (2010)

Supporting Distributed Shared Memory (DSM) is essential for multi-core Network-on-Chips for the sake of reusing huge amount of legacy code and easy programmability. We propose a microcoded controller as a hardware module in each node to connect the core, the local memory and the network. The controller is programmable where the DSM functions such as virtual-to-physical address translation, memory access and synchronization etc. are realized using microcode. To enable concurrent processing of memory requests from the local and remote cores, our controller features two mini-processors, one dealing with requests from the local core and the other from remote cores. Synthesis results suggest that the controller consumes 51k gates for the logic and can run up to 455 MHz in 130 nm technology. To evaluate its performance, we use synthetic and application workloads. Results show that, when the system size is scaled up, the delay overhead incurred by the controller may become less significant when compared with the network delay. In this way, the delay efficiency of our DSM solution is close to hardware solutions on average but still have all the flexibility of software solutions.
Finding Structural Information About RF Power Amplifiers Using an Orthogonal Nonparametric Kernel Smoothing Estimator (2015)

A nonparametric technique for modeling the behavior of power amplifiers (PAs) is presented. The proposed technique relies on the principles of density estimation using the kernel method and is suited for use in PA modeling. The proposed methodology transforms the input domain into an orthogonal memory domain. In this domain, nonparametric static functions are discovered using the kernel estimator. These orthogonal nonparametric functions can be fitted with any desired mathematical structure, thus facilitating its implementation. Furthermore, due to orthogonality, the nonparametric functions can be analyzed and discarded individually, which simplifies pruning basis functions and provides a tradeoff between complexity and performance. The results show that the methodology can be employed to model PAs, therein yielding error performance similar to state-of-the-art parametric models. Furthermore, a parameter-efficient model structure with six coefficients was derived for a Doherty PA, therein significantly reducing the deployment's computational complexity. Finally, the methodology can also be well exploited in digital linearization techniques.
Fractionated stereotactic radiosurgery in rat brain: histopathological determination of dose-response and tolerance (2006)

Fractionated stereotactic radiosurgery (fSRS) allows single or multiple high dosage radiation of small volumes within the brain, i.e. addressing intracranial tumors. Reasonable protection of perilesional brain parenchyma raises clinical interest in fSRS strategies and promising results were already achieved for numerous focal lesions (e.g.: arteriovenous malformations, brain metastases, small benign tumors and malignancies). The effect of hyperfractionated stereotactic radiosurgery on healthy brain tissue (e.g.: apoptosis, necrosis, impact on the blood-brain-barrier) remains, however, to be determined.
PAC-Membrane Filtration Process. I: Model Development (2000)

Adsorption is modeled when powdered activated carbon (PAC) is applied in continuous-flow reactors followed by membrane filtration units operated without carbon wastage between backwash events. Four reactor configurations are studied: (1) A membrane reactor dosed with a step input of PAC; (2) a continuous-flow stirred tank reactor dosed with a step input of PAC and followed by a membrane reactor; (3) a plug-flow reactor dosed with a step input of PAC and followed by a membrane reactor; and (4) a membrane reactor dosed with a pulse input of PAC at the beginning of the filtration cycle. A steady-state operation is considered to describe the adsorption process through the continuous-flow stirred tank reactor and plug-flow reactor, whereas adsorption in the membrane reactor is modeled as a non-steady-state process. Adsorption kinetics is assumed to occur by homogeneous surface diffusion, and adsorption equilibrium is described with the Freundlich isotherm model. Analytical solutions of the homogeneous surface diffusion model with no external mass transfer limitation are used to evaluate adsorbate concentrations in the solid phase as a function of time. Part II of this study presents model simulations and verification with experimental data obtained in a bench-scale apparatus.
Minimizing Regret With Label Efficient Prediction (2005)

We investigate label efficient prediction, a variant, proposed by Helmbold and Panizza, of the problem of prediction with expert advice. In this variant, the forecaster, after guessing the next element of the sequence to be predicted, does not observe its true value unless he asks for it, which he cannot do too often. We determine matching upper and lower bounds for the best possible excess prediction error, with respect to the best possible constant predictor, when the number of allowed queries is fixed. We also prove that Hannan consistency, a fundamental property in game-theoretic prediction models, can be achieved by a forecaster issuing a number of queries growing to infinity at a rate just slightly faster than logarithmic in the number of prediction rounds.
Characterization of Ni thin films following thermal oxidation in air (2014)

The authors study the thermal oxidation of nickel thin films (50 nm) fabricated by conventional thermal evaporation, resulting from annealing in air at 300, 325, 350, 400, and 700 °C. The characterization is performed by x-ray diffraction, Raman spectroscopy, superconducting quantum interference device magnetometry, and scanning electron microscopy. These techniques show that the oxidation increases with annealing temperature. The formation of granular films of coexisting Ni and NiO is confirmed after annealing at 400 °C. The magnetic measurements indicate coexisting ferromagnetism and antiferromagnetism, corresponding to Ni and NiO contributions. The magnetic hysteresis loops reveal exchange bias in the samples annealed at 235, 350, and 400 °C due to the competition between the exchange interactions at the Ni/NiO interfaces.
Study on Wide-band Voltage Controlled Current Source for Electrical Impedance Tomography (2012)

Electrical Impedance Tomography is applied to acquire the impedance characteristic, which is correlative with the physiological and pathological status of a body, and reconstruct the images of impedance distribution. Current injection with voltage measurement phantom is widely adopted in the application of biomedical detection. Voltage controlled current source (VCCS) develops an important part and its performance directly affects the precision of the system. In this paper we analyze and simulate the driving capability and operating bandwidth of the improved Howland current source. By using a wide-band, low noise amplifier, OPA843, we implement the current excitation circuit, which performs well. The maxim output impedance achieves 103.41Mohm under frequency from 1KHz to 100KHz. When driving a pure resistive load of 2Kohm, the -3dB bandwidth is 63MHz and decreases to 10.7MHz with resistive load of 10Kohm. When the output impedance decreases due to the stray capacitance, a general impedance converter is used in parallel with Howland. The simulation and test results show that the voltage controlled current source performs stably and provides great output impedance and superior driving bandwidth, which is suitable for EIT application.
SURVEY AND ANALYSIS OF MEDIA LITERACY EDUCATION IN COLLEGE STUDENTS OF SCIENCE AND ENGINEERING OF HUNAN PROVINCE (2010)

Improving the media literacy and enhancing the media literacy education become more and more urgent.The survey of media literacy education in college students of science and engineering of Hunan province,shows that they tend to rational use of media resources.However,they rarely understand the basics of media and lack the ability of media criticism.Moreover,they lack awareness of media literacy education and this leads to that they lack the motivation to media literacy education.