gebhart/patrepeval huggingface dataset:
Rapid Identification of Bacterial Virulence Factors (2014)

Abstract : The main objective of this work is to demonstrate that Rapid Virulence Factor Discovery System (RVFDS) can be used to identify virulence factors of MRSA and Brucella abortus. This will be accomplished by constructing an MRSA and B. abortus random expression library (REL) in E. coll. The MRSA REL contains hundreds of potential virulence factors. Putative virulence factors that interacted with the ECM components fibronectin, laminin, and plasminogen, collagen, and anti-MRSA polyclonal antibodies will be eluted from pull-down columns that contain these bait proteins. Putative MRSA virulence factors will be identified by LC-MS/MS. Additional virulence factors will be identified using a wider range of ECM components as bait proteins. In the Phase I investigation the average size of the REL insert was in the 1.5 to 2.0kb range. In Phase II the average size of the insert will be increased to 3.0kb in order to decrease redundancy. Larger inserts and REL protein sizes will also facilitate LC-MS/MS identification and shorten the time needed for data analysis. Potential proteins that are discovered in Phase II work may serve as targets for future vaccines, antimicrobials, and therapeutic measures.
The USC CARE corpus: child-psychologist interactions of children with autism spectrum disorders (2011)

We introduce the USC CARE Corpus, comprised of spontaneous and standardized child-psychologist interactions of children with a diagnosis of an autism spectrum disorder (ASD). The audio-video data is collected in the context of the Autism Diagnostic Observation Schedule (ADOS), which is a tool used by psychologists for a research-level diagnosis of ASD for children. The interaction consists of developmentally appropriate semi-structured social activities, providing the psychologist with a sample of behavior used to rate the child on a series of autism-relevant symptoms. Our goal with this multimodal corpus is to investigate how analytical technology (e.g., speech and language processing) can enhance this observational rating task and provide greater insight into social behavior and communication. We provide demographic statistics on the recruited children (60 to date), describe the multimodal recording set-up, and discuss current and future work for this novel corpus. Index Terms: autism, autism spectrum disorders, children’s speech, corpus, multimodal recordings, prosody
Highly flexible ultrafast laser system with 260W average power (2011)

A flexible ultrafast laser amplifier system based on Ytterbium Innoslab technology with an average power exceeding 200W is presented. The pulse duration of the system can be continuously tuned between 500fs and 6ps, limited only by the amplification bandwidth of Yb:YAG and the stretcher of the seed source. The repetition rate can be varied from 26.6MHz down to 1MHz. For the ps-regime more than 200&mu;J and for the fs-regime more than 50&#956;J are demonstrated without the need of temporal compression of the high power beam after the amplifier. Spectral bandwidth is close to the transform limit of the shortest measured pulses. Beam quality is measured to be near the diffraction limit (M<sup>2</sup>&lt;1.3).
Cytoprotective Properties of a New Nanocomplex of Selenium with Taxifolin in the Cells of the Cerebral Cortex Exposed to Ischemia/Reoxygenation (2022)

The neuroprotective effect of the natural antioxidant taxifolin (TAX) is well known for ischemic pathologies. However, the limitations of taxifolin application are described-poor solubility, low ability to penetrate the blood-brain barrier, and side effects from high doses for stroke therapy. We proposed the problem of targeted delivery of taxifolin and achievement effective concentrations could be solved by developing a nanocomplex of selenium nanoparticles (SeNPs) with taxifolin (Se-TAX). In this study, we developed a selenium-taxifolin nanocomplex based on selenium nanoparticles with a 100 nm size. It was shown that TAX, SeNPs, and Se-TAX were all able to suppress the production of ROS in neurons and astrocytes under exposure to exogenous H<sub>2</sub>O<sub>2</sub> and ischemia-like conditions. However, the Se-TAX nanocomplex appeared to be the most effective, displaying a lower working concentration range and negligible pro-oxidant effect compared with pure SeNPs. The mechanism of Se-TAX beneficial effects involved the activation of some antioxidant enzymes and the suppression of ROS-generating systems during OGD/reoxygenation, while TAX and "naked" SeNPs were less effective in regulating the cellular redox status. Naked SeNPs inhibited a global increase in Ca<sup>2+</sup> ions in cytosol, but not OGD-induced hyperexcitation of the neuroglial network, while Se-TAX suppressed both [Ca<sup>2+</sup>]i rise and hyperexcitation. The effect of TAX at similar doses appeared exclusively in inhibiting OGD-induced hyperexcitation. Analysis of necrosis and apoptosis after OGD/reoxygenation revealed the highest efficiency of the Se-TAX nanocomplex as well. Se-TAX suppressed the expression of proinflammatory and proapoptotic proteins with simultaneous activation of protective genes. We conclude that the Se-TAX nanocomplex combines the antioxidative features taxifolin and the antiapoptotic effect of nanoselenium, involving the regulation of Ca<sup>2+</sup> dynamics.
Upregulation of Irisin and Vitamin D-Binding Protein Concentrations by Increasing Maternal 25-Hydrovitamin D Concentrations in Combination with Specific Genotypes of Vitamin D-Binding Protein Polymorphisms (2021)

Dyshomeostasis of vitamin D-binding protein (VDBP) has been implicated in the pathogenesis of various pregnancy complications, including preeclampsia, preterm birth, gestational diabetes, and adverse metabolic profiles in the offspring. VDBP polymorphisms have been consistently reported to contribute to this intriguing interplay. Until recently, the effects of VDBP polymorphism heterogeneity on maternal and neonatal adipomyokine profiles have not been investigated, specifically after incorporating the different maternal and neonatal 25-hydroxyvitamin D concentration cut-offs at birth. We aimed to investigate the potential effects of maternal and neonatal VDBP polymorphisms on adiponectin, irisin, and VDBP concentrations at birth, according to different cut-offs of vitamin D status, in maternal-neonatal dyads recruited from the sunny region of Northern Greece. We obtained blood samples from 66 mother-child pairs at birth. Results indicated that (i) Neonatal serum biomarkers were not affected by any included neonatal VDBP polymorphism according to different cut-offs of neonatal vitamin D status at birth, (ii) neonatal VDBP concentration was elevated in neonates with maternal rs7041 GG genotype, (iii) maternal 25(OH)D at ≤75 nmol/L resulted in increased concentrations of maternal VBDP and irisin concentrations in women with CC genotype for rs2298850 and rs4588,whereas this effect was also evident for this cut-off for neonatal VDBP concentrations at birth for GC genotype for rs 7041, and (iv) no significant effect of neonatal VDBP polymorphisms was observed on neonatal VDBP, adiponectin, or irisin levels when stratified according to maternal 25(OH)D cut-offs. In conclusion, these findings confirm that among women with the combination of CC genotype for rs2298850 and rs4588, a specific high cut-off of maternal 25(OH)D results in increasing maternal VBDP concentrations, hence providing a mechanistic rationale for aiming for specific cut-offs of vitamin D after supplementation during pregnancy, in daily clinical practice.
Heart-lung transplantation: Cardiac clinicopathological correlations (1996)

Reports on heart-lung transplantation emphasize the pathology of the transplanted lungs. This study is a clinicopathological assessment of cardiac pathology in the hearts transplanted as part of the combined heart-lung block. Seventy-five consecutive heart-lung transplants (H-LTx) performed between 1981 and 1989 were studied. Endomyocardial biopsy, autopsy and clinical data were analyzed for information on cardiac rejection, graft coronary disease, transplant survival and the presence of obliterative bronchiolitis and compared with controls. The controls consisted of 391 heart transplants (HTx) performed in 361 recipients over the same time period. Sixty-three adults and nine children received H-LTx (48.6% male; 51.4% female). In this study, H-LTx were performed primarily for Eisenmenger's complex (33 72 ) and primary pulmonary hypertension (28 72 ). At 1 year H-LTx survival was 63.88%, versus 81.54% in HTx alone and 63.63% in lung transplant recipients without heart grafts. The results showed that H-LTx patients have less cardiac rejection compared to patients who undergo HTx alone (p < .005). Only 40% of H-LTx recipients developed acute cardiac rejection in the initial 3 months posttransplantation (post-Tx), compared with 80% of HTx controls. Only 4% of H-LTx recipients developed cardiac rejection after the initial 6 months. No cardiac rejection was observed in the initial 5 years post-Tx in 49.9% of H-LTx. Graft coronary disease was seen in 7.73% of H-LTx within the first 5 years post-Tx compared with 25.87% in HTx recipients alone (p < .005). Obliterative bronchiolitis (OB) was present in 71.43% of H-LTx with graft coronary disease, compared to OB in only 41.38% of H-LTx without graft coronary disease (p < .05). In conclusion, H-LTx recipients have less acute cardiac rejection episodes than HTx recipients alone (p < .005). Most cardiac rejection in H-LTx occurs within the initial 6 months. In addition, H-LTx recipients develop less graft coronary disease than HTx recipients (p < .005). Obliterative bronchiolitis and graft coronary disease may be the result of the same immunological process, as 71.43% of H-LTx with graft coronary disease also had OB. Survival for H-LTx patients is more similar to that of lung transplant (LTx) patients alone than that of heart transplants alone, suggesting that it is the pulmonary pathology portion, rather than the cardiac pathology portion, in combined H-LTx transplants that contributes more to H-LTx survival.
INTERIOR OF SOLAR AND EXTRASOLAR GIANT PLANETS (2009)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Ronald Redmer, Nadine Nettelmann, Ulrike Kramm, Bastian Holst, Winfried Lorenzen, Martin French; INTERIOR OF SOLAR AND EXTRASOLAR GIANT PLANETS. AIP Conf. Proc. 28 December 2009; 1195 (1): 905–910. https://doi.org/10.1063/1.3295290 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Coating of porous silica beads by in situ polymerization/crosslinking of 2‐hydroxypropyl β‐cyclodextrin for reversed‐phase high performance liquid chromatography applications (2003)

Abstract Porous silica beads were coated with a crosslinked β‐cyclodextrin polymer by in situ polymerization/crosslinking of 2‐hydroxypropyl β‐cyclodextrin with 1,6‐hexamethylenediisocyanate in anhydrous dimethylsulfoxide. This method was developed for the preparation of reversed‐phase high performance liquid chromatography stationary phases. The mass of polymer immobilized onto the silica surface was controlled by the amount of coupling agent, 1,6‐hexamethylenediisocyanate, added during the coating process. The influence of the polymer coating on the physical features of the beads was investigated by means of nitrogen adsorption/desorption methods, scanning electron microscopy and energy dispersion X‐ray analysis. The column lifetime was found to be primarily dependent on the extent of crosslinking of the stationary phase. Moreover, it was demonstrated that the synthesis of highly crosslinked stationary phases with a reasonable column lifetime gave rise to a phase separation of the β‐cyclodextrin polymer. To prove their usefulness as reversed‐phase packing materials, they were used to separate mixtures of nitrophenol positional isomers, four pesticides, and drugs. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 91: 1419–1426, 2004
IL-10 and TGF-β Control the Establishment of Persistent and Transmissible Infections Produced by Leishmania tropica in C57BL/6 Mice (2008)

Leishmania tropica is the causative agent of Old World anthroponotic cutaneous leishmaniasis, which is characterized by lesions that take an extended period of time to heal, often resulting in disfiguring scars, and are more refractory to treatment than leishmaniasis caused by Leishmania major. Immunologic studies involving experimental animal models of L. tropica infection are virtually nonexistent. In the current study, infectious-stage L. tropica were used to establish dermal infections in C57BL/6 and BALB/c mice. In both strains, the lesions were slow to develop and showed minimal pathology. They nonetheless contained a stable number of between 10(4) and 10(5) parasites for over 1 year, which were efficiently picked up by a natural sand fly vector, Phlebotomus sergenti. Control of parasite growth depended on the development of a Th1 response, as C57BL/6 mice genetically deficient in Th1 cytokines and BALB/c mice treated with Abs to IFN-gamma harbored significantly more parasites. By contrast, IL-10-deficient mice harbored significantly fewer parasites throughout the infection. To further study the immunologic mechanisms that may prevent efficient clearance of the parasites, IL-10 and TGF-beta signaling were abrogated during the chronic phase of infection in wild-type C57BL/6 mice. Distinct from chronic L. major infection, IL-10 blockade alone had no effect on L. tropica, but required simultaneous treatment with anti-TGF-beta Abs to promote efficient parasite clearance from the infection site. Thus, chronic infection with L. tropica appears to be established via multiple suppressive factors, which together maintain the host as a long-term reservoir of infection for vector sand flies.
H2S Oxidative Decomposition Reaction in the Presence of CH4 over Metal-Sulfide-Based Catalysts: A Preliminary Investigation (2021)

Different alumina-supported metal sulfides catalysts were studied for the H2S oxidative decomposition reaction to simultaneously obtain sulfur and hydrogen in the presence of CH4. Mono and bimetallic catalysts were prepared, characterized, and tested at 1273 K by varying the contact time. X-ray diffraction and Raman evidenced a good dispersion of the Mo, Fe, and Co sulfide species on the Al2O3 support. From the experimental results, it was argued that Mo sulfide species are the active phases able to inhibit the formation of SO2, while the Fe sulfide species promote the selective abatement of COS and CS2 obtained because of the presence of CH4 in the gaseous feed stream. The bimetallic sample containing both Mo and Fe sulfide species (5Fe-10Mo/Al2O3) exhibited very interesting catalytic performances since it allowed us to achieve a H2 yield, CH4, and H2S conversion very close to the equilibrium values and the complete removal of sulfur compounds at low contact times.
Enhanced UI Automator Viewer with improved Android accessibility evaluation features (2016)

Inaccessible user interfaces of the applications in Android systems such as unlabeled interactive controls, lack of form element hint, lack of focus on the important interactive controls, improper directional navigation etc. are hurdles of users with all kinds of disabilities. Manual accessibility testing is tedious job for Applications developers. Also efficient semi automated Android accessibility evaluation tool which can evaluate all the accessibility developer's checklist are not available. These could be important reasons of non consideration of accessibility while developing Android applications. This paper presents enhancement of UI Automator Viewer a tool for evaluation of applications accessibility in Android environment. Limitation of UI Automator Viewer are eliminated by adding new features “Target device selection”, which allows the developer to select target device among multiple connected devices for automation testing, “Capture and play”, include Touch Event and key Event which help to send UI event to and retrieve information from connected device and “Color contrast” which shows color contrast of captured screenshot which helps the mobile application developer to represent their application for color blind and low vision persons and to make sure that all visual designs meet the minimum color-contrast ratio for normal and large text on a background. Testing accessibility of user interfaces of Android mobile applications become more efficient by the enhanced tool which can identify accessibility issues of user interfaces of Android applications and assist developers to create accessible user interfaces.
Synthesis and in situ foaming of biodegradable malonic acid ESO polymers (2008)

Abstract In this study, biodegradable rigid cellular materials were synthesized from the reaction of malonic acid with epoxidized soybean oil. Malonic acid reacts with two epoxy groups to give a network polymer. In the course of this reaction, initially formed malonic acid monoester (MAME) can decarboxylate and produce CO 2 , which acts as the blowing agent leading to in situ foaming of the polymer. Epoxide addition and decarboxylation reactions of MAME occur competitively and simultaneously and by controlling their relative rates, foams of controlled density were produced. 1 H NMR spectrum of the synthesized foams showed that increasing the temperature increases the rate of decarboxylation reaction of MAME and decreases crosslink density leading to softer and lower density foams. Addition of 1,4‐diazabicyclo[2.2.2]octane (DABCO) as a catalyst also increases the rate of decarboxylation. Load deflection curves of the cellular materials showed that decreasing the temperature and addition of DABCO increase compressive modulus of samples. Cell morphology was studied by microscopic images of foam samples that showed that foam samples have a closed cell structure and a wide distribution of cell volume. Soil burial test was done to determine rate of biodegradation of foam samples. A half‐life of 815 days showed that foam samples are highly biodegradable. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Unambiguous state discrimination in quantum cryptography with weak coherent states (2000)

The use of linearly independent signal states in realistic implementations of quantum key distribution (QKD) enables an eavesdropper to perform unambiguous state discrimination. We explore quantitatively the limits for secure QKD imposed by this fact taking into account that the receiver can monitor, to some extent the photon-number statistics of the signals even with todays standard detection schemes. We compare our attack to the beam-splitting attack and show that security against the beam-splitting attack does not necessarily imply security against the attack considered here.
Laboratory Testing Fails to Reproduce the Demanding Conditions Imposed on the Knee by Active Individuals (2012)

Introduction Knee prostheses retrieved at revision often show patterns and severity of damage neither seen nor predicted from standard wear simulator testing. We hypothesized that this is because these implants are exposed to combinations of loads and motions that are more damaging than the simple loading profiles utilized in laboratory testing. We examined the magnitude, direction, and combination of forces and moments acting on the knee during various activities in order to guide the future development and testing of high-performance knee replacements. Methods In vivo data from five patients with instrumented tibial implants were obtained from an open database (www.orthoload.com). We determined the direction and magnitude of forces and moments that the knee experiences during the following common physiologic activities: stair descent, stair ascent, deep knee bend, one leg stance, and walking. In order to capture the loading pattern, we investigated the three component forces and moments acting on the knee at several high demand points for each of these activities. The e-tibia data were compared to the loading profiles used in conventional laboratory testing (ISO 14243-1). Results The vast majority of maximum forces and moments measured during these activities far exceeded those applied during laboratory testing, often by several-fold (Table 1). Analysis of loading profiles showed considerable differences in the loading patterns both between individuals and activities. At the point of peak axial force during level walking, there were four distinct loading patterns in five patients- none of which matched the laboratory testing pattern. The comparison of the median loading pattern at the point of maximum axial force showed that each of the five activities generated distinct loading patterns, which all differed substantially from the loading pattern applied during conventional knee testing. Discussion Current routines for laboratory testing of total knee joint prostheses fail to develop forces and moments of the magnitude present within knee prostheses and surrounding interfaces during physiologic activities. Moreover, the combinations of force and moment components generated during conventional testing differ fundamentally from those occurring in vivo. These discrepancies may explain the differences between the wear patterns seen in components retrieved at revision versus those generated by laboratory simulators. Clearly, new testing protocols imposing more severe loading conditions and variable loading patterns are required to simulate service conditions generated by more active patients and more diverse activities after TKR.
The Impact of Mismatch on the Performance of Coded Narrow-Band FM with Limiter/Discriminator Detection (1983)

The performance of uncoded narrow-band FM with limiter/discriminator (L/D) detection over the additive white Gaussian noise channel has received widespread attention [1]-[14] over the years both from simulation and analytical standpoints. By comparison, little or nothing has appeared in the literature for the performance of this modulation/demodulation technique when used together with coding/decoding. The purpose of this paper is to partially fill this void by considering the potential gain offered by the combination of convolutionally encoded narrow-band FM with L/D detection and Viterbi decoding. Both hard and soft decision decoding cases will be considered with some rather surprising differences between the performances of the two. In particular, it will be shown that a large departure exists from the 2 dB advantage traditionally associated with soft decision decoding which is explained in terms of the mismatch between the coding channel and the decoding metric peculiar to this modulation/demodulation technique.
The Phenomenon of Neutrophil Extracellular Traps in Vascular Diseases (2018)

Vascular diseases constitute a global health issue due to the increasing number of cases of patients with these diseases. The pathogenesis of the majority of these diseases, including atherosclerosis and thrombosis, is complex and not yet fully understood. One of the major causes for their occurrence can be immune disorders resulting in the development of a chronic inflammation within the vessels. In recent years, studies have placed emphasis on the role of neutrophils in the development of these diseases, i.e., the discovery of neutrophil extracellular traps (NETs) demonstrated that the structures released by the cells may contribute to the enhancement of inflammatory reactions and cell damage. This article summarizes current knowledge on the role of NETs during atherosclerosis, thrombosis and small-vessel vasculitis, especially in antineutrophil cytoplasmic antibody (ANCA)-associated small-vessel vasculitis (AAV).