gebhart/patrepeval huggingface dataset:
Quantitative analysis of leptin mRNA using competitive reverse transcription polymerase chain reaction and capillary electrophoresis with laser-induced fluorescence detection (2000)

Leptin, the protein hormone product of the obese (ob) gene, functions in the regulation of appetite, energy expenditure, and reproduction in animals and humans. Since changes in the level of circulating leptin can have marked physiological consequences, it is important to be able to accurately quantify leptin gene expression. Toward this goal, we have constructed a chicken leptin RNA competitor and successfully employed it as an internal standard in the development of a quantitative-competitive reverse transcription polymerase chain reaction (QC-RT-PCR) assay for leptin mRNA. Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was utilized for the separation and analysis of chicken leptin target (261 bp) and competitor (234 bp) dsDNA products from QC-RT-PCR assay samples. Leptin amplicons were separated using a DB-1 coated capillary (27 cm x 100 microm ID) at a field strength of 300 V/cm in a replaceable sieving matrix consisting of 0.5% hydroxypropylmethyl cellulose (HPMC) in 1 x TBE (89 mM Tris-base, 89 mM boric acid, 2 mM EDTA, pH 8.3) buffer with 0.5 microg/mL EnhanCE fluorescent intercalating dye. Samples were diluted 1:100 with deionized water and introduced into the capillary by electrokinetic injection. QC-RT-PCR/CE-LIF was used to quantify leptin mRNA in liver and adipose tissue from 8-week-old male and female broiler chickens. This study is the first report of quantitative analysis of leptin gene expression using QC-RT-PCR/CE-LIF.
Semiclassical Transport Theory (2019)

Modern Condensed Matter Physics brings together the most important advances in the field of recent decades. It provides instructors teaching graduate-level condensed matter courses with a comprehensive and in-depth textbook that will prepare graduate students for research or further study as well as reading more advanced and specialized books and research literature in the field. This textbook covers the basics of crystalline solids as well as analogous optical lattices and photonic crystals, while discussing cutting-edge topics such as disordered systems, mesoscopic systems, many-body systems, quantum magnetism, Bose–Einstein condensates, quantum entanglement, and superconducting quantum bits. Students are provided with the appropriate mathematical background to understand the topological concepts that have been permeating the field, together with numerous physical examples ranging from the fractional quantum Hall effect to topological insulators, the toric code, and majorana fermions. Exercises, commentary boxes, and appendices afford guidance and feedback for beginners and experts alike.
Supplementary Figure 5 from Six1 Overexpression in Ovarian Carcinoma Causes Resistance to TRAIL-Mediated Apoptosis and Is Associated with Poor Survival (2023)

Supplementary Figure 5 from Six1 Overexpression in Ovarian Carcinoma Causes Resistance to TRAIL-Mediated Apoptosis and Is Associated with Poor Survival
Analysis for the subcellular distribution of oligonucleotide/lipid complexes. (1997)

We synthesized a lipoglutamide having tetraethylene glycol tails (PEG lipid), and prepared a DNA/PEG lipid complex. Observation by a confocal laser scanning fluorescence microscope showed that the oligonucleotide/PEG lipid complex distributed in cytoplasmic region. On the other hand, a oligonucleotide/lipofectin complex distributed in nucleus. Interactions of the oligonucleotide complexes with isolated nucleus were also investigated, and mechanisms of the subcellular distributions were discussed.
Interference cancellation matrix beamforming for 3-D beamspace ML/MUSIC bearing estimation (1991)

For the case of two closely spaced targets angularly located within the mainlobe of the transmitted beam, 3D-BDML in a computationally simplistic maximum likelihood bearing estimation scheme which operates in a 3-D beamspace generated by three orthogonal, classical beamformers. The presence of strong interferers angularly located outside the mainlobe encompassing the two targets of interest necessitates the use of adaptively formed left, center, and right beams. Let M denote the number of elements comprising the array. Novel procedures are developed for the construction of and M*3 interference cancellation matrix beamformer which retains those properties of the M*3 classical matrix beamformer critical to the computational simplicity of 3D-BDML. The most important of these is commonality of M-3 nulls among the left, center, and right beams. Simulations demonstrating the performance of both single frequency and multifrequency 3D-BDML are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Online network impedance identification with wave-package and inter-harmonic signals (2015)

In power system analysis the main supply at a point of common coupling (PCC) is often presented by its Thévenin or Norton equivalent. In most cases these models adequately define the electrical characteristics of a PCC and are commonly used for power flow evaluations, fault estimations, as well as stability and power quality assessments. To determine the equivalent impedance of a PCC through analytical calculation or simulation precisely may be challenging due to the complexity and time varying nature of power supply systems. Especially in medium- and low-voltage distribution networks with a high share of fluctuating consumers and generators a measurement may be required to exactly capture the equivalent impedance over time. This contribution presents a method, which can be used to identify varying equivalent parameters of an AC power system with high accuracy and time resolution. To identify the network impedance the system is excited with short sequences of current signals while measuring the corresponding voltage and current characteristics. The method is evaluated in a simulation model. Further on, impedance measurements on the low-voltage level are presented and discussed.
A low-power CMOS super-regenerative receiver at 1 GHz (2001)

A low-power and low-voltage super-regenerative receiver operating at 1 GHz and implemented in a 0.35-/spl mu/m CMOS process is described. The receiver includes a low-noise amplifier, a super-regenerative oscillator, an envelope detector, an AGC circuit with sample/hold function, and a baseband amplifier. The die surface is equal to 0.25 mm/sup 2/. The power consumption is less than 1.2 mW at V/sub DD/=1.5 V. A 100-kHz sawtooth quench signal is used to achieve a rejection of -36 dB at 500 KHz from the central frequency.
Clinician blood pressure documentation of stable intensive care patients: An intelligent archiving agent has a higher association with future hypotension (2011)

During periods of hemodynamic stability in an intensive care unit patient population, clinician-documented blood pressure values were inferior to values from an intelligent automated archiving method as early indicators of hemodynamic instability. Human oversight may not be necessary for creating a valid archive of vital sign data within an electronic medical record. Furthermore, if clinicians do have a tendency to disregard early indications of instability, then an automated archive may be a preferable source of data for so-called early warning systems that identify patients at risk of decompensation.
Relationship between the sublingual microcirculation and lactate levels in patients with heart failure (2008)

In order to find out the frequency rates of domestic and wild animal bites as well as the evaluation of the prevalence rates of rabies disease in the human population in the Province of Kerman, a retrospective study was designed to analyze statistically the collected recorded data related to this project. Methods This study was conducted within the framework of MPVM student research projects by means of collaboration between
PDCT-03. PHASE 1 STUDY OF Ad-RTS-hIL-12 + VELEDIMEX IN PEDIATRIC BRAIN TUMORS (2017)

Pediatric glioblastoma has an aggressive clinical course accounting for significant morbidity and mortality among children with brain tumors. Only 10% of children with DIPG survive for 2 years following diagnosis, and less than 1% survive for 5 years. The median survival is 9 months from diagnosis. New therapies are urgently needed. Ad-RTS-hIL-12 is a novel gene therapy expressing IL-12 under the control of an oral activator ligand, veledimex, via the RheoSwitch Therapeutic System® gene switch. Intratumoral administration of Ad-RTS-hIL-12 results in targeted tumor cytotoxicity and induction of systemic T-cell memory. Ad-RTS-hIL-12 + veledimex is a treatment strategy to extend the IL-12 therapeutic window. Based on encouraging data from an ongoing phase I dose escalation trial in adults with recurrent/progressive glioma and preclinical data in a GL-261 medullary glioma mouse model, we expect significant safety margins and tolerability profile in pediatric glioma. In a phase 1 adult study, subjects received intratumoral injections of Ad-RTS-hIL-12 2x1011 viral particles during resection or administered stereotactically. Daily oral veledimex began prior to surgery or following stereotactic injection. Overall, Ad-RTS-hIL-12 + veledimex in adults was well tolerated; toxicities were predictable and reversible upon discontinuing veledimex with a correlation between veledimex dose, BBB penetration and drug related AEs. The tolerability and encouraging survival observed warrants a dose escalation pediatric trial for subjects with recurrent/progressive brain tumors. Two groups of subjects receiving Ad-RTS-hIL-12 2x1011 viral particles are planned: Arm 1 receives Ad-RTS-hIL-12 intratumorally after resection of supratentorial tumors that are unresponsive to conventional treatment or for which there is no alternative curative therapy; Arm 2 consists of stereotactic injection in subjects with DIPG post prior standard focal radiotherapy and for which a diagnostic biopsy has previously been obtained. Subjects receive BSA adjusted escalating doses of 10 or 20mg veledimex. An update will be presented.
Neurocognitive and behavioral outcomes in Latino childhood cancer survivors (2013)

In addition to the child's age at diagnosis, acculturation appears to predict select neurocognitive and behavioral outcomes in this socio-demographically homogeneous sample of Latino families. Further research is needed to understand the interaction of ethnic and cultural factors with therapeutic exposures in determining the adverse neurobehavioral outcomes, so as to optimally design interventions.
Microstructural characterization in plates using guided wave third harmonic generation (2014)

Presently, good understanding of second harmonic generation in a weakly nonlinear plate has been obtained, yet the limited number of primary excitations constrains the use of second harmonics in field measurements. The cumulative characteristics of third harmonic generation in a cubic nonlinear plate has been investigated. The power flux analysis shows that primary wave fields are capable of generating a cumulative third harmonic that is of the same nature. Further synchronism analysis shows that the primary shear-horizontal modes are holo-internal-resonant with third harmonic fields, which will be advantageous in experimental measurements. A nonlinear finite element simulation confirms that the primary SH1 mode generates a cumulative sh3 third harmonic mode.
Pharmacogenomics and Therapeutic Target Validation in Cerebral Vasospasm (2000)

One of the most important pharmacogenomic technologies is transcriptome analysis. We used this method to study the change of gene expression profiles in animal models of cerebral vasospasm. We found novel drug target candidates in cerebral vasospasm through pharmacogenomics. By using differential display and quantitative reverse transcriptase-polymerase chain reaction, we found that heme oxygenase-1 (HO-1) mRNA was prominently induced in the basilar artery and modestly in brain tissue in a murine vasospasm model. There was a significant correlation between the degree of vasospasm and HO-1 mRNA levels in the basilar arteries exhibiting vasospasm. Antisense HO-1 oligodeoxynucleotides (ODN) inhibited HO-1 induction in the basilar arteries, but not in the whole brain tissue. This phenomenon was not observed in the nontreatment, sense HO-1 ODN and scrambled ODN treatment arteries. We report, for the first time, the protective effects of HO-1 gene induction by endogenous or clinical compounds in cerebral vasospasm after subarachnoid hemorrhage, a finding that should provide a novel therapeutic target for cerebral vasospasm.
A Quantitative Systems Approach Reveals Dynamic Control of tRNA Modifications during Cellular Stress (2010)

Decades of study have revealed more than 100 ribonucleoside structures incorporated as post-transcriptional modifications mainly in tRNA and rRNA, yet the larger functional dynamics of this conserved system are unclear. To this end, we developed a highly precise mass spectrometric method to quantify tRNA modifications in Saccharomyces cerevisiae. Our approach revealed several novel biosynthetic pathways for RNA modifications and led to the discovery of signature changes in the spectrum of tRNA modifications in the damage response to mechanistically different toxicants. This is illustrated with the RNA modifications Cm, m(5)C, and m(2) (2)G, which increase following hydrogen peroxide exposure but decrease or are unaffected by exposure to methylmethane sulfonate, arsenite, and hypochlorite. Cytotoxic hypersensitivity to hydrogen peroxide is conferred by loss of enzymes catalyzing the formation of Cm, m(5)C, and m(2) (2)G, which demonstrates that tRNA modifications are critical features of the cellular stress response. The results of our study support a general model of dynamic control of tRNA modifications in cellular response pathways and add to the growing repertoire of mechanisms controlling translational responses in cells.
NMR study of copper with vanadium impurities (1977)

We present NMR data on copper with dilute vanadium impurities. We have measured the Knight shift of the $^{51}\mathrm{V}$ impurity resonance and analyzed it in terms of a nonmagnetic virtual bound state. We have detected several host satellite resonances. One has a very large quadrupole coupling constant ${\ensuremath{\nu}}_{Q}\ensuremath{\sim}3.5$ MHz and asymmetry parameter $\ensuremath{\eta}\ensuremath{\sim}0.3$; we identify it as the first-neighbor resonance. A magnetic satellite with shift $\frac{\ensuremath{\Delta}K}{K}=\ensuremath{-}0.658\ifmmode\pm\else\textpm\fi{}0.015$ is thought to be the resonance of the second or fourth neighbors. A satellite with weak magnetic coupling $\frac{\ensuremath{\Delta}K}{K}=+0.2\ifmmode\pm\else\textpm\fi{}0.1$ is probably due to third neighbors. Our studies conclude that vanadium impurities in copper are magnetically similar to cobalt impurities.
Anglofonia, literaturas em línguas africanas e limites da teoria pós-colonial (2010)

A partir da leitura do conto “A meeting in the dark”, de Ngũgĩ wa Th iong’o, que epitomiza o choque entre tradicao e modernidade na Africa colonial, este artigo discute o papel social das literaturas africanas produzidas em linguas autoctones em contextos onde a lingua inglesa fi gura-se como hegemonica e as limitacoes da teoria pos-colonial enquanto disciplina que busca estudar, compreender e atenuar os dilemas do sujeito colonizado.
lac repressor blocks in vivo transcription of lac control region DNA. (1987)

Transcription of the Escherichia coli lac repressor gene (lacI) in vivo produces monocistronic mRNAs with discrete 3' ends in the lac control region, although the DNA sequence of this region does not specify a strong termination signal of the traditional form. Direct analysis of lac transcripts was used to show that the DNA sequence alone does not provide the signal to end the repressor mRNA and to establish that of the proteins with specific binding sites on control region DNA only the lac repressor has a striking effect on the continuity of lacI gene transcription. RNAs with 3' ends in the control region sequence are major mRNA species produced from a repressor-bound template, reflecting as much as a 50-fold increase over their levels in the repressor's absence. Repressor binding to the operator thus has a dual function. In addition to blocking initiation of transcription from the lacZ promoter, repressor serves as a termination factor by setting the length of its own transcript and separating lacI and lacZYA into two distinct transcription units.
Cardiovascular autonomic nervous system dysfunction in patients with rheumatoid arthritis and systemic lupus erythematosus (1999)

Although peripheral and central nervous system involvement have been well recognized in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), autonomic nervous system (ANS) involvement has rarely been studied, and has shown conflicting results. We performed cardiovascular ANS assessment in 34 RA and 37 SLE patients, using standard cardiovascular reflex tests. The results in each patient were compared with age- and sex-matched healthy controls. Forty-seven percent of the RA patients and 19% of the SLE patients had symptoms suggesting ANS dysfunction. The heart rate variation in response to deep breathing was significantly decreased in both the RA and SLE patients (p = 0.001). This diminished heart rate response showed no correlation with the disease duration, the number of swollen joints, the Ritchie articular index, ESR, or rheumatoid factor in the RA group, or the disease duration, the SLEDAI score or ESR in the SLE group. The clinical significance of the diminished cardiovascular ANS response needs to be investigated.
KNOX1 genes regulate lignin deposition and composition in monocots and dicots (2013)

Plant secondary cell walls are deposited mostly in vascular tissues such as xylem vessels, tracheids, and fibers. These cell walls are composed of a complex matrix of compounds including cellulose, hemicellulose, and lignin. Lignin functions primarily to maintain the structural and mechanical integrity of both the transport vessel and the entire plant itself. Since lignin has been identified as a major source of biomass for biofuels, regulation of secondary cell wall biosynthesis has been a topic of much recent investigation. Biosynthesis and patterning of lignin involves many developmental and environmental cues including evolutionarily conserved transcriptional regulatory modules and hormonal signals. Here, we investigate the role of the class I Knotted1-like-homeobox (KNOX) genes and gibberellic acid in the lignin biosynthetic pathway in a representative monocot and a representative eudicot. Knotted1 overexpressing mutant plants showed a reduction in lignin content in both maize and tobacco. Expression of four key lignin biosynthesis genes was analyzed and revealed that KNOX1 genes regulate at least two steps in the lignin biosynthesis pathway. The negative regulation of lignin both in a monocot and a eudicot by the maize Kn1 gene suggests that lignin biosynthesis may be preserved across large phylogenetic distances. The evolutionary implications of regulation of lignification across divergent species are discussed.
Model-based, event-driven programming paradigm for interactive web applications (2013)

Applications are increasingly distributed and event-driven. Advances in web frameworks have made it easier to program standalone servers and their clients, but these applications remain hard to write. A model-based programming paradigm is proposed that allows a programmer to represent a distributed application as if it were a simple sequential program, with atomic actions updating a single, shared global state. A runtime environment executes the program on a collection of clients and servers, automatically handling (and hiding from the programmer) complications such as network communication (including server push), serialization, concurrency and races, persistent storage of data, and queuing and coordination of events.
The Research on the Mechanism of Limiting Speed Pick-Up and Set-Out Train on Railway Transportation Capacity Loss (2009)

Pick-up and set-out trains refer to these trains marshaled in technological station, serving for vehicles flow in the district, whose pick-up and set-out operations are done in intermediate stations in the district. Running of limiting speed pick-up and set-out trains has an impair on railway transport capacity and research on railway transport capacity loss mechanism for limiting speed pick-up and set-out trains is the core content of that on railway transport capacity, which has an extremely significance on reasonable organization vehicle flow and improvement the traffic efficiency. The factors of railway transport capacity loss caused by limiting speed pick-up and set-out trains are analyzed in order to explore the relationship among these factors. From different levels, factors that contribute to railway transport capacity loss are also analyzed quantitatively. The cellular automata theory are applied to build up cellular space and change rules of railway transport capacity loss for limiting speed pick-up and set-out trains as well as to describe the cellular states in the study. With computer simulation, the capacity loss characters of limiting speed pick-up and set-out trains are depicted and the loss mechanism of railway transport capacity caused by limiting speed pick-up and set-out trains is also put forward. Based on these, the evaluation model and algorithm of railway transport capacity loss for limiting speed pick-up and set-out trains are devised to calculate the capacity loss. Finally, given the district between Shanghai Fuliji-Eastern Bengbu, the capacity loss rate of limiting speed pick-up and set-out trains is calculated to evaluate railway transport capacity loss in the district.
c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin. (1994)

We have cloned a mouse cDNA that is a member of the Krox gene family and encodes a protein we have named c-Krox. The c-Krox protein contains three zinc fingers of the Cys2His2 type. c-Krox binds specifically to a guanine-rich cis-acting element present twice in the promoter element of the mouse alpha 1(I) collagen gene. Study of c-Krox gene expression shows that c-Krox is markedly enriched in skin, one of the two major sites of type I collagen synthesis, but is absent in bone, the other main type I collagen-producing tissue, indicating that type I collagen gene expression is differentially regulated in skin and bone. DNA transfection experiments in mouse NIH 3T3 fibroblasts, cells that express the c-Krox gene, or in Drosophila S2 cells, which do not express c-Krox, reveal that c-Krox can activate transcription of a reporter gene linked to several copies of its binding site in the alpha 1(I) collagen promoter. Thus, c-Krox is only the second member of the Krox family for which a target gene has been identified. The selective spatial pattern of expression of its mRNA and its transcription activation ability suggest that c-Krox may be an important regulator of type I collagen skin specific expression in physiologic conditions and in fibrotic diseases such as scleroderma.
Vector-running Subcuticular Sutures in High-Tension and High-Mobility Areas (1992)

Routine cuticular sutures pierce the epidermis, and if left in place long enough, will noticeably scarify, a process called "tracking." Alternatively, the running subcuticular suture has the advantage of minimizing suture tracking, especially following a prolonged usage, because it does not pierce the epidermis along most of its course. A "hybrid" modification of these two sutures, described herein, allows for prolonged tight closure under high tension in high-movement areas, avoidance of tracking, and rapidity of placement. This innovation is called the vector-running subcuticular suture.