gebhart/patrepeval huggingface dataset:
Structurally integrated transmitter beacon for underwater wireless optical communications in multiple ocean types (2021)

In this paper we describe a structurally integrated optical transmitter beacon concept that consists of a side-scattering fiber that can conform to solid surfaces, such as the outer surface of a submersible Remotely Operated Vehicle (ROV), suitable for convenient deployment in underwater applications. By coupling a modulated optical signal from a laser diode into the fiber, an omnidirectional âbeaconâ is achieved. We demonstrate coarse Wavelength Division Multiplexing (WDM), illustrating that these beacons can transmit optical wireless data through several attenuation lengths in turbid water at aggregate data rates of up to 20 Mb/s.
RNA Transcription from Immobilized DNA Templates (1995)

We describe an RNA transcription protocol based on the multiple reuse of solid-phase synthetic DNA templates. The templates are assembled onto streptavidin-coated agarose beads via a single 5'-terminal biotin located on the noncoding template strand. Transcription occurs in an aqueous buffered suspension containing solid-phase DNA, dissolved enzyme (T7 RNA polymerase), and nucleoside triphosphate substrates (NTPs). A direct comparison of solution and solid-phase templates under standard transcription conditions reveals similar initial reaction rates and overall yields. Immobilized templates store stably for periods of several months and are easily recovered by mild centrifugation. We demonstrate the successive reuse of these templates throughout 15 rounds of transcription. The templates remained active, although an incremental decay in transcription was observed beyond five rounds. Template activity was partially restored by supplementing the support-bound oligonucleotide with fresh coding-strand DNA. These findings indicate that multiple reuse of template is a viable strategy for reducing the amount of DNA template required in RNA transcription.
Prostasin Is Required for Matriptase Activation in Intestinal Epithelial Cells to Regulate Closure of the Paracellular Pathway (2013)

The type II transmembrane serine protease matriptase is a key regulator of epithelial barriers in skin and intestine. In skin, matriptase acts upstream of the glycosylphosphatidylinositol-anchored serine protease, prostasin, to activate the prostasin zymogen and initiate a proteolytic cascade that is required for stratum corneum barrier functionality. Here, we have investigated the relationship between prostasin and matriptase in intestinal epithelial barrier function. We find that similar to skin, matriptase and prostasin are components of a common intestinal epithelial barrier-forming pathway. Depletion of prostasin by siRNA silencing in Caco-2 intestinal epithelium inhibits barrier development similar to loss of matriptase, and the addition of recombinant prostasin to the basal side of polarized Caco-2 epithelium stimulates barrier forming changes similar to the addition of recombinant matriptase. However, in contrast to the proteolytic cascade in skin, prostasin functions upstream of matriptase to activate the endogenous matriptase zymogen. Prostasin is unable to proteolytically activate the matriptase zymogen directly but induces matriptase activation indirectly. Prostasin requires expression of endogenous matriptase to stimulate barrier formation since matriptase depletion by siRNA silencing abrogates prostasin barrier-forming activity. Active recombinant matriptase, however, does not require the expression of endogenous prostasin for barrier-forming activity. Together, these data show that matriptase and not prostasin is the primary effector protease of tight junction assembly in simple columnar epithelia and further highlight a spatial and tissue-specific aspect of cell surface proteolytic cascades.
Beneficial effects of leptin on obesity, T cell hyporesponsiveness, and neuroendocrine/metabolic dysfunction of human congenital leptin deficiency (2002)

The wide range of phenotypic abnormalities seen in the leptin-deficient ob/ob mouse and their reversibility by leptin administration provide compelling evidence for the existence of multiple physiological functions of this hormone in rodents. In contrast, information regarding the roles of this hormone in humans is limited. Three morbidly obese children, who were congenitally deficient in leptin, were treated with daily subcutaneous injections of recombinant human leptin for up to 4 years with sustained, beneficial effects on appetite, fat mass, hyperinsulinemia, and hyperlipidemia. Leptin therapy resulted in a rapid and sustained increase in plasma thyroid hormone levels and, through its age-dependent effects on gonadotropin secretion, facilitated appropriately timed pubertal development. Leptin deficiency was associated with reduced numbers of circulating CD4(+) T cells and impaired T cell proliferation and cytokine release, all of which were reversed by recombinant human leptin administration. The subcutaneous administration of recombinant human leptin has major and sustained beneficial effects on the multiple phenotypic abnormalities associated with congenital human leptin deficiency.
Synthesis and drug controlled release of block copolymers of poly(l‐lactide) with poly(d, l‐lactide) and related monomers (1997)

Abstract Four types of new biodegradable block copolymers AB, ABA, AC and AD, where A is poly(L‐lactide) (PLLA), B is poly(D, L‐lactide) (PDLLA), C is poly(p‐dioxanone) (PDON) and D is poly(ϵ‐caprolactone) (PCL) with different block lengths were synthesized and characterized by GPC, IR, 1 H‐NMR and DSC. There are phase‐separated and biodegradable block copolymers. Their in vitro biodegradation rates with the change of composition ratio were studied as well as the biodegradation rates of homopolymers with the series as PDON &gt; PDLLA &gt; PLLA in parallel with their crystallinities, i.e. from amorphous to semicrystalline. All these block copolymers were used as matrix to test their controlled release behavior of levo‐norgestrel (LNG) in the form of microspheres through solvent evaporation preparation with thoroughly long‐time washing to minimize the generally occurring bursting effect. As a result all of them showed almost constant rate of release even from the initial stage,
Topography-guided versus wavefront-optimized laser in situ keratomileusis for myopia: Surgical outcomes (2019)

Although both topography-guided LASIK and wavefront-optimized LASIK safely and effectively achieved the predicted refractive and visual outcomes, topography-guided LASIK induced fewer HOAs and significantly decreased ocular trefoil, corneal total HOAs, and coma.
Minimizing Complications of Aging That Lead to Dry Mouth and Poor Oral Health (2021)

Abstract Poor oral health causes severe pain and untreated infections to spread throughout the body. For older adults, the prevalence of root decay exceeds that of any other medical condition. Our research shows tooth loss and edentulousness were associated with increased mortality and inversely associated with BMI, waist circumference, blood pressure, and fasting blood glucose. Our Stop-it study found people who lost bone density had fewer teeth, problems chewing, and involuntary weight loss and frailty. 88% of the elderly take medications that cause loss of saliva. Sjögren’s and radiation therapy for head and neck cancer patients heighten risk. Without saliva, patients have increased tooth decay, periodontal disease and fungal infections, salivary gland blockage, and problems swallowing and speaking. Dry mouth leads people to suck on candy that further increase caries. Substituting sugarfree gum for candy increases salivary flow and reduces dental caries. Brushing, flossing, and limiting sugar also lessen tooth decay.
Novel Gyrotron Beam Annealing Method for Mg-Implanted Bulk GaN (2019)

Here we present for the first time the use of gyrotron beam for selective annealing/activation of implanted Mg in bulk GaN films. Samples with 1 μm uGaN epi-Iayer grown by MOCVD on HVPE bulk n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> GaN substrates were implanted with Mg (1 x 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">19</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">−3</sup> ) to a target depth of 500 nm. A systematic investigation of annealing temperature, N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> overpressure, surface capping layer, and sample orientation to the beam are presented. Post-implantation, volumetric lattice deformation was recovered by performing continuous annealing at temperatures <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$&gt;$</tex> 1100 °C for 30 s. Both AIN capped and uncapped samples were studied. It is shown that the surface of uncapped GaN samples which were annealed at 1100 °C in 40 bar N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> overpressure remained intact. Photoluminescence spectroscopy was performed and confirmed the incorporation of Mg in substitutional sites with this technique, along with formation of large density of nitrogen vacancy defects.
The Effect of Upscaling and Performance Degradation on Onshore Wind Turbine Lifetime Extension Decision Making (2017)

Ever greater rated wind turbine generators (WTGs) are reaching their end of design life in the near future. In addition, first research approaches quantified the impact of long-term performance degradation of WTGs. As a consequence, this work is aimed at discussing and analysing the impact of upscaling and performance degradation on the economics of wind turbine lifetime extension. Findings reveal that the lifetime extension levelised cost of energy (LCOE2) of an 18 MW wind farm comprising of 0.5 MW rated WTGs are within the order of £23.52 per MWh. Alternatively, if the same wind farm consists of fewer 2 or 3 MW WTGs, the LCOE2 reduces to £16.56 or £15.49 per MWh, respectively. Further, findings reveal that an annual performance degradation of 1.6% (0.2%) increases LCOE2 by 34-41% (3.6-4.3%).
Programming with C (2009)

About the Book: Authors have taken special care to present the various topics in Programming with C++ in an easy-to-learn style. Almost every topic is followed by well designed live programmes so that it becomes easy to grasp the underlying principle or programming technique. A total of more than 450 live programmes are included in the book. It is also taken care that programmes are short and do not include such details which do not relate to the topic on hand. This makes them easy to be tested and suitable for practice students. Authors are confident that the book will prove its worth for th
UNAFLOW: a holistic wind tunnel experiment about the aerodynamic response of floating wind turbines under imposed surge motion (2021)

Abstract. Floating offshore wind turbines are subjected to large motions due to the additional degrees of freedom of the floating foundation. The turbine rotor often operates in highly dynamic inflow conditions, and this has a significant effect on the overall aerodynamic response and turbine wake. Experiments are needed to get a deeper understanding of unsteady aerodynamics and hence leverage this knowledge to develop better models and to produce data for the validation and calibration of existing numerical tools. In this context, this paper presents a wind tunnel experiment about the unsteady aerodynamics of a floating turbine subjected to surge motion. The experiment results cover blade forces, rotor-integral forces, and wake. The 2D sectional model tests were carried out to characterize the aerodynamic coefficients of a low-Reynolds-number airfoil with harmonic variation in the angle of attack. The lift coefficient shows a hysteresis cycle close to stall, which grows in strength and extends in the linear region for motion frequencies higher than those typical of surge motion. Knowledge about the airfoil aerodynamic response was utilized to define the wind and surge motion conditions of the full-turbine experiment. The global aerodynamic turbine response is evaluated from rotor-thrust force measurements, because thrust influences the along-wind response of the floating turbine. It is found that experimental data follow predictions of quasi-steady theory for reduced frequency up to 0.5 reasonably well. For higher surge motion frequencies, unsteady effects may be present. The turbine near wake was investigated by means of hot-wire measurements. The wake energy is increased at the surge frequency, and the increment is proportional to the maximum surge velocity. A spatial analysis shows the wake energy increment corresponds with the blade tip. Particle image velocimetry (PIV) was utilized to visualize the blade-tip vortex, and it is observed that the vortex travel speed is modified in the presence of surge motion.
EZH2 as a Regulator of CD8+ T Cell Fate and Function (2020)

Enhancer of zeste 2 (EZH2) is the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2) that mediates di- and trimethylation of histone 3 lysine 27 effectively precluding successful gene transcription at these loci. This class of epigenetic modifications facilitates the maintenance of tissue-specific cellular transcriptional programs as cells undergoing successive rounds of proliferation. CD8+ T cells are effective mediators of adaptive immunity and function to eliminate virus- and bacteria-infected cells as well as tumor cells. Upon recognition of cognate antigen, T cells undergo activation/proliferation to clear the target cells. The heterogeneous population of responding T cells formed during these proliferative events thus rely on epigenetic modifications to ensure identity and confer functional capabilities. In this review, we will focus on the role of the dynamic expression EZH2 in shaping the epigenetic landscape of CD8+ T cell fate and function, with a particular emphasis on infection and cancer. We also explore competing hypotheses pertaining to EZH2 function and the prospects of clinical EZH2 inhibitors in fine-tuning T cell responses.
Multiplex real-time RT-qPCR for the detection of Norovirus in bottled and tap water using murine norovirus as a process control (2013)

Given the increasing evidence for NoV involvement in food outbreaks, the one-step multiplex RT-qPCR assay we used in this study would be a very useful tool to investigate NoV contamination in other food products.
Classification and regression tree analysis of acute‐on‐chronic hepatitis B liver failure: Seeing the forest for the trees (2016)

At present, there is no ideal model for predicting the short-term outcome of patients with acute-on-chronic hepatitis B liver failure (ACHBLF). This study aimed to establish and validate a prognostic model by using the classification and regression tree (CART) analysis. A total of 1047 patients from two separate medical centres with suspected ACHBLF were screened in the study, which were recognized as derivation cohort and validation cohort, respectively. CART analysis was applied to predict the 3-month mortality of patients with ACHBLF. The accuracy of the CART model was tested using the area under the receiver operating characteristic curve, which was compared with the model for end-stage liver disease (MELD) score and a new logistic regression model. CART analysis identified four variables as prognostic factors of ACHBLF: total bilirubin, age, serum sodium and INR, and three distinct risk groups: low risk (4.2%), intermediate risk (30.2%-53.2%) and high risk (81.4%-96.9%). The new logistic regression model was constructed with four independent factors, including age, total bilirubin, serum sodium and prothrombin activity by multivariate logistic regression analysis. The performances of the CART model (0.896), similar to the logistic regression model (0.914, P=.382), exceeded that of MELD score (0.667, P<.001). The results were confirmed in the validation cohort. We have developed and validated a novel CART model superior to MELD for predicting three-month mortality of patients with ACHBLF. Thus, the CART model could facilitate medical decision-making and provide clinicians with a validated practical bedside tool for ACHBLF risk stratification.
Phase I clinical trial of the use of zinc phthalocyanine tetrasulfonate as a photosensitizer for photodynamic therapy in dogs (2007)

A conservative starting dose of ZnPcS(4) was arrived at on the basis of mouse toxicosis findings. Zinc phthalocyanine tetrasulfonate-based PDT was tolerated well by all dogs and warrants further study. The identification of the maximum tolerated dose through traditional phase I clinical trials may be unnecessary for evaluating novel PDT protocols.
Aero Engine Test Experience With CMSX-4® Alloy Single Crystal Turbine Blades (1994)

A team approach involving a turbine engine company [Rolls-Royce], its single crystal casting facilities and a superalloy developer and ingot manufacturer [Cannon-Muskegon], utilizing the concepts of simultaneous engineering, has been used to successfully develop CMSX-4 alloy for turbine blade applications. CMSX-4 alloy is a second generation nickel-base single crystal superalloy containing 3% (wt) rhenium (Re) and 70% volume fraction of the coherent γ′ precipitate strengthening phase. Its finely balanced composition offers an attractive range of properties for turbine airfoil applications. In particular the alloy’s combination of high strength in relation to creep-rupture, mechanical and thermal fatigue, good phase stability following extensive high temperature, stressed exposure and oxidation, hot corrosion and coating performance, are attractive for turbine engine applications where engine performance and turbine airfoil durability are of prime importance. The paper details the single crystal casting process and heat treatment manufacturing development for turbine blades in CMSX-4 alloy. Competitive single crystal casting yields are being achieved in production and extensive vacuum heat treatment experience confirms CMSX-4 alloy to have a practical production solution heat treat / homogenization “window”. The creep-rupture data-base on CMSX-4 alloy now includes 325 data points from seventeen heats including fourteen 3630 kg (8000 lb) production size heats. An appreciable portion of this data was machined-from-blade (MFB) properties which indicate turbine blade component capabilities based on single crystal casting process, component configuration and heat treatment. The use of hot-isostatic-pressing (HIP) has been shown to eliminate single crystal casting micropores which along with the essential absence of γ/γ′ eutectic phase, carbides, stable oxide, nitride and sulphide inclusions results in remarkably high mechanical fatigue properties, with smooth and particularly notched specimens. The Re addition has been shown to not only benefit creep and mechanical fatigue strength (with and without HIP), but also bare oxidation, hot corrosion (sulphidation) and coating performance. The high level of balanced properties determined by extensive laboratory evaluation has been confirmed during engine testing the Rolls-Royce Pegasus turbofan.
MoO3/Ag/MoO3 anode in organic photovoltaic cells: Influence of the presence of a CuI buffer layer between the anode and the electron donor (2012)

MoO3/Ag/MoO3 (MAM) multilayer structures (layers thickness 20 nm/10 nm/35 nm) are used as anode in CuPc/C60/Alq3/Al organic photovoltaic cells. The averaged transmittance (400 nm-800 nm) of these MoO3/Ag/MoO3 multilayer structures is 70% ± 2% and their sheet resistance is 3.5 ± 1.0 Ω/sq. When these multilayer structures are used as anode, the power conversion efficiency of the MoO3/Ag/MoO3/CuPc/C60/Alq3/Al cells is around 1%, this efficiency is increased of 50% when a thin CuI film (3 nm) is introduced at the interface between the anode and the organic film. This improvement is attributed to the templating effect of CuI on the CuPc molecules.
Transcriptionally Driven DNA Replication Program of the Human Parasite Leishmania major (2016)

Faithful inheritance of eukaryotic genomes requires the orchestrated activation of multiple DNA replication origins (ORIs). Although origin firing is mechanistically conserved, how origins are specified and selected for activation varies across different model systems. Here, we provide a complete analysis of the nucleosomal landscape and replication program of the human parasite Leishmania major, building on a better evolutionary understanding of replication organization in Eukarya. We found that active transcription is a driving force for the nucleosomal organization of the L. major genome and that both the spatial and the temporal program of DNA replication can be explained as associated to RNA polymerase kinetics. This simple scenario likely provides flexibility and robustness to deal with the environmental changes that impose alterations in the genetic programs during parasitic life cycle stages. Our findings also suggest that coupling replication initiation to transcription elongation could be an ancient solution used by eukaryotic cells for origin maintenance.
Plasma frequency tuning of epsilon-near-zero metafilm with ITO-SiO2 multilayer structure (2022)

In this summary, we report an experimental demonstration of plasma frequency tuning of a multilayer-based ENZ metafilm. The demonstrated ENZ metafilm consists of alternating layers of ITO and SiO2. The optical properties, such as the effective plasma frequency and damping coefficients, of the demonstrated metafilm were controlled by changing the volume fraction of the metal layer. The measured effective permittivity values from an ellipsometry analysis show good agreement with the calculated results using a simple Maxwell-Garnett effective medium theory.
AJA method and AJA Canvas as a Design Tool for Autonomous Operations (2018)

Several design methods and principles have been presented so far, in order to guide the design of autonomous operations. Putting the required efforts into learning and using the methods for designing autonomous operations is a daunting task. Experiences so far have shown that the use of methods meant to the help the design process are often ignored. One reason could be that the design guidelines are too complex and contain much information often not relevant for the project at hand, and therefore there is no easy way to distinguish what is important from what is not. This is an issue that needs to be solved with our approach. In this article, the Autonomous Job Analysis (AJA) method is presented. The proposed methodology is created in order to guide the design of autonomous operations in maritime systems by breaking them down in to sub-operations in order to reveal challenges, needs and limitations regarding autonomous behavior. The canvas contains the categories of the AJA method on a single page format -the canvas- and each category is supported with questions to be asked during the design procedure, as well as example answers. We will describe the AJA method and the AJA canvas in detail, and present a use case scenario of an autonomous operation in order to show how they can be applied. The particular use-case is the design of an autonomous operation for the detection, inspection and tracking of a waste water plume.