gebhart/patrepeval huggingface dataset:
Construction of a Structurally Defined Double-Stranded DNA Catenane (2011)

Topologically interlocked structures like catenanes and rotaxanes are promising components for the construction of molecular machines and motors. Herein we describe the construction of double-stranded DNA catenanes for DNA nanotechnology. For this, C-shaped DNA minicircle fragments were equipped with sequence-specific DNA-binding polyamides and their respective binding site. Formation of catenanes is achieved by self-assembly of two of these fragments and subsequent addition of a ring-closing oligonucleotide.
GH, GH Receptor, GH Secretagogue Receptor, and Ghrelin Expression in Human T Cells, B Cells, and Neutrophils (2001)

We examined GH and GH receptor expression in human leukemic cell lines and leukocytes of normal subjects to elucidate the cell types expressing GH and GH receptor, the individual variations of their expressions, their correlation and the relationships with serum IgG and IGF-I concentrations. In addition, the expression of GH secretagogue receptor, which enhances GH secretion from the anterior pituitary by synthetic GH secretagogues and that of its endogenous ligand, ghrelin, were also examined in these immune cells. GH expression in human leukemic cell lines was observed mainly in B cell lines at both the mRNA and protein level [3.8 +/- 0.2 pg/10(6) cells in Raji and 19.9 +/- 3.3 pg/10(6) cells in Daudi vs. negligible in T cell lines (Jurkat and Hut-78) and in myeloid cell lines (K-562 and HL-60)]. B cells in normal subjects were also found to be the major immune cells expressing GH mRNA, with significant individual variation. GH receptor mRNA expression was detectable in all human leukemic cell lines, although the expression level varied widely among the cell lines and was weaker than that in the liver. On the other hand, GH receptor mRNA expression was mainly found in B cells, with marked individual variation in normal subjects. There was a positive correlation between the mRNA expressions of GH and GH receptor in B cells of normal subjects (r = 0.89; P < 0.001). Single cell RT-PCR revealed that some B cells expressed both GH and GH receptor transcripts, and others expressed only GH. GH/GH receptor expression levels in B cells did not show any correlation with serum IgG and IGF-I levels in normal subjects. Expression of GH secretagogue receptor and ghrelin was detectable in all immune cells regardless of the maturity and cell types with great individual variations. In summary, GH secreted from B cells may act locally on their own receptors, and their variable expressions may be related to individual immune functions. Widespread distribution of ghrelin and GH secretagogue receptor in human immune cells may indicate unknown biological functions other than enhancing GH secretion in the immune system.
Non-clairvoyant multiprocessor scheduling of jobs with changing execution characteristics (extended abstract) (1997)

Article Non-clairvoyant multiprocessor scheduling of jobs with changing execution characteristics (extended abstract) Share on Authors: Jeff Edmonds Department of Computer Science, York University, North York, ONT M3J 1P3 Department of Computer Science, York University, North York, ONT M3J 1P3View Profile , Donald D. Chinn Department of Computer Science, York University, North York, ONT M3J 1P3 Department of Computer Science, York University, North York, ONT M3J 1P3View Profile , Tim Brecht Department of Computer Science, York University, North York, ONT M3J 1P3 Department of Computer Science, York University, North York, ONT M3J 1P3View Profile , Xiaotie Deng Department of Computer Science, York University, North York, ONT M3J 1P3 Department of Computer Science, York University, North York, ONT M3J 1P3View Profile Authors Info & Claims STOC '97: Proceedings of the twenty-ninth annual ACM symposium on Theory of computingMay 1997 Pages 120–129https://doi.org/10.1145/258533.258565Online:04 May 1997Publication History 16citation278DownloadsMetricsTotal Citations16Total Downloads278Last 12 Months3Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Modernizing ASTM D2272 (RPVOT) Criteria for Long Service Life Duration Oils (2022)

Turbine and other large machines require lubrication where considerable volumes of oil remain in service for numerous years between replacements. The service life for these applications is primarily influenced by oxidation stability and the residues that this degradation creates. Oxidation stability is one of the most critical factors used in assessing the performance of fresh and in-service oils. A primary condition monitoring test method utilized to measure the oil oxidative stability is the rotating pressure vessel oxidation test (RPVOT), which is described in ASTM D2272. The oxidative resistance of modern turbine oils has increased dramatically over the last 50 years. Advances in lubrication technologies such as improved base oil formulations, additive synergy and synthetic oils have extended RPVOT test times from less than 300 min to over 3,000 min. This has caused extended testing runtime that exceeds the original intent of the method and the long test durations have potentially introduced errors that affect the accuracy and predictive capability of the test. As a result, the ability to properly trend and assess the relative oxidative health of the oil may be compromised. The D2272 Test Method was examined using alternative reporting criteria expected to improve test precision and provide a more accurate assessment of a lubricants condition. Reporting criteria based upon the true induction point and pressure drop at a specified time were examined. Efficacy of using pressure loss from the test maximum at 1,400 minutes for condition monitoring purposes was evaluated and holds promise. These changes would be expected to improve accuracy for full induction tests or lead to laboratory cost savings in that tests can be terminated earlier and without an induction occurring; effectively increasing laboratory throughput and provide the end user with more informative data that is more befitting for long life oils in use today.
Fate of Listeria monocytogenes in tissues of experimentally infected cattle and in hard salami (1988)

Muscle, organ, and lymphoid tissues of four Holstein cows experimentally inoculated (intravenously) with Listeria monocytogenes were examined 2, 6, or 54 days postinoculation for the presence of the organism by direct plating and cold enrichment procedures. L. monocytogenes was isolated from 66% of the tissues sampled; 38% of the isolations were attributed to the use of cold enrichment. Isolation of the organism from muscle tissue was possible only with animals inoculated 2 days before slaughter. The fate of L. monocytogenes during the manufacture and storage of fermented hard salami made from this meat also was determined. Three sausage treatments were evaluated: (i) uninoculated control sausage, (ii) "naturally" contaminated sausage (NC) made from meat of an experimentally inoculated cow, and (iii) sausage made from beef inoculated with a laboratory culture of L. monocytogenes (I). Initial Listeria levels in NC and I sausage were 10(3) CFU/g in trial 1 and 10(4) CFU/g in trial 2. Numbers of L. monocytogenes decreased by approximately 1 log10 CFU/g during fermentation and decreased further during drying and refrigerated storage. Small numbers (less than or equal to 20 CFU/g) of L. monocytogenes were present in I and NC sausage at the end of 12 weeks of refrigerated storage; recovery of these organisms generally depended on the use of an enrichment procedure. The results indicate that L. monocytogenes does not multiply during the fermentation and drying processes typical of hard salami manufacture but that survival may occur if the organism is initially present at greater than or equal to 10(3) CFU/g.
A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology (2019)

Urodele newts have unique biological properties, notably including prominent regeneration ability. The Iberian ribbed newt, Pleurodeles waltl, is a promising model amphibian distinguished by ease of breeding and efficient transgenic and genome editing methods. However, limited genetic information is available for P. waltl. We conducted an intensive transcriptome analysis of P. waltl using RNA-sequencing to build and annotate gene models. We generated 1.2 billion Illumina reads from a wide variety of samples across 12 different tissues/organs, unfertilized egg, and embryos at eight different developmental stages. These reads were assembled into 1,395,387 contigs, from which 202,788 non-redundant ORF models were constructed. The set is expected to cover a large fraction of P. waltl protein-coding genes, as confirmed by BUSCO analysis, where 98% of universal single-copy orthologs were identified. Ortholog analyses revealed the gene repertoire evolution of urodele amphibians. Using the gene set as a reference, gene network analysis identified regeneration-, developmental-stage-, and tissue-specific co-expressed gene modules. Our transcriptome resource is expected to enhance future research employing this emerging model animal for regeneration research as well as for investigations in other areas including developmental biology, stem cell biology, and cancer research. These data are available via our portal website, iNewt (http://www.nibb.ac.jp/imori/main/).
Operation Altitude Optimization of Solar-Powered Rotary-Wing UAVs for FSO Backhauling (2023)

Free space optical communication (FSO) has emerged as an alternative backhauling technology. It provides a line-of-sight (LOS) link with a capacity comparable to fiber optics and much higher than those that can be supported by radio counterparts. Rotary-wing unmanned aerial vehicles (UAVs) equipped with FSO terminals can be positioned as a complementary aerial solution to the terrestrial backhaul links in dense areas with high-peak traffic demands. In this paper, we consider a solar-powered rotary-wing UAV equipped with an FSO terminal that provides backhaul link to a ground base station in an urban area. We first quantity the energy consumption and energy harvesting of a rotary-wing solar-powered UAV. Then, we formulate an optimization problem to determine the optimal operation altitude with the goal of maximizing the net energy of UAV while satisfying the LOS requirements critical for the FSO link. Our results show that the selection of operation altitude is highly dependent on the weight of the UAV as well as the size and efficiency of the solar panel.
Influence of the Synthesis Additive on the Textural and Structural Characteristics of Sol−Gel Al2O3−TiO2 (2000)

Al2O3−TiO2 materials were prepared by the sol−gel method using different additives (HNO3, NH4OH, and CH3COOH) and their physical properties were evaluated by various techniques. The materials at two compositions (Al/Ti atomic ratios = 2 and 25) were synthesized at 278 K and calcined at temperatures from 573 to 1173 K. The solids were characterized by TGA, DTA, XRD, BET, and SEM. The complexing (CH3COOH) and the basic (NH4OH) additives led to solids with high pore volumes and broad pore size distributions. On one hand, very high surface areas (525 m2/g for solids calcined at 773 K) were found for samples prepared with CH3COOH. On the other hand, high-temperature stability was obtained with NH4OH addition (200 m2/g, for Al2O3-rich samples at 1173 K). HNO3-catalyzed samples showed lower surface areas and pore volumes. Surface areas and sintering behavior were a function of TiO2 content. TiO2-rich samples showed higher surface areas (773 K) than Al2O3-rich oxides, but at more severe conditions they suffered a severe specific area loss. The alumina-rich formulations showed good stability in the whole range of temperatures studied.
Semiconductor Gamma-Cameras in Nuclear Medicine (1980)

The availability of semiconductor detectors, principally High Purity Germanium, permits consideration of new generations of nuclear medicine imaging devices. These are characterized by excellent spatial resolution and by simultaneous multiple radioisotope imaging capabilities. The technology and some of its resultant benefits are presented here.
Development and Optimization of a GMP-Compliant Manufacturing Process for a Personalized Tumor Lysate Dendritic Cell Vaccine (2020)

With the emergence of immune checkpoint inhibitors and adoptive T-cell therapies, there is a considerable interest in using personalized autologous dendritic cell (DC) vaccines in combination with T cell-targeting immunotherapies to potentially maximize the therapeutic impact of DC vaccines. Here, we describe the development and optimization of a Good Manufacturing Practice (GMP)-compliant manufacturing process based on tumor lysate as a tumor antigen source for the production of an oxidized tumor cell lysate loaded DC (OC-DC) vaccine. The manufacturing process required one day for lysate preparation and six days for OC-DC vaccine production. Tumor lysate production was standardized based on an optimal tumor digestion protocol and the immunogenicity was improved through oxidation using hypochloric acid prior to freeze-thaw cycles resulting in the oxidized tumor cell lysate (OC-L). Next, monocytes were selected using the CliniMACS prodigy closed system and were placed in culture in cell factories in the presence of IL-4 and GM-CSF. Immature DCs were loaded with OC-L and matured using MPLA-IFNγ. After assessing the functionality of the OC-DC cells (IL12p70 secretion and COSTIM assay), the OC-DC vaccine was cryopreserved in multiple doses for single use. Finally, the stability of the formulated doses was tested and validated. We believe this GMP-compliant DC vaccine manufacturing process will facilitate access of patients to personalized DC vaccines, and allow for multi-center clinical trials.
One‐Step Room‐Temperature Synthesis of Metal(IV) Carboxylate Metal—Organic Frameworks (2020)

Abstract Room‐temperature syntheses of metal–organic frameworks (MOFs) are of interest to meet the demand of the sustainable chemistry and are a pre‐requisite for the incorporation of functional compounds in water‐stable MOFs. However, only few routes under ambient conditions have been reported to produce metal(IV)‐based MOFs. Reported here is a new versatile one‐step synthesis of a series of highly porous M 6 ‐oxocluster‐based MOFs (M=Zr, Hf, Ce) at room temperature, including 8‐ or 12‐connected micro/mesoporous solids with different functionalized organic ligands. The compounds show varying degrees of defects, particularly for 12‐connected phases, while maintaining the chemical stability of the parent MOFs. Proposed here are first insights into in situ kinetics observations for efficient MOF preparation. Remarkably, the synthesis has a high space‐time yield and also provides the possibility to tune the particle size, therefore paving the way for their practical use.
Modeling the Photochemistry of the Reference Phototoxic Drug Lomefloxacin by Steady‐State and Time‐Resolved Experiments, and DFT and Post‐HF Calculations (2007)

The irradiation in water of 1-ethyl-6,8-difluoro-7(3-methylpiperazino)3-quinolone-2-carboxylic acid (lomefloxacin), a bactericidal agent whose use is limited by its serious phototoxicity (and photomutagenicity in the mouse), leads to formation of the aryl cation in position eight that inserts into the 1-ethyl chain. Trapping of the cation was examined and it was found that chloride and bromide straightforwardly add in position eight, but with iodide and with pyrrole the 1-(2-iodoethyl) and the 1-[2-(2-pyrrolyl)ethyl] derivatives are formed. Flash photolysis reveals the triplet of lomefloxacin, a short-lived species (lambda max=370 nm, tau=40 ns) that generates the triplet cation (lambda max=480 nm, tau approximately 120 ns). The last intermediate is quenched both by halides and by pyrrole. DFT and post-HF methods have shown that the triplet is the lowest state of the cation (Delta G(ST)=13.3 kcal mol(-1)) and intersystem crossing (ISC) to the singlet has no role because a less endothermic process occurs, that is, intramolecular hydrogen abstraction from the N-ethyl chain (9.2 kcal mol(-1)) that finally leads to cyclization. The halides form weak complexes with the triplet cation (kq from 4.9 x 10(8) for Cl(-) to 7.0 x 10(9) m(-1) s(-1) for I-). With Cl(-) and Br(-) ISC occurs in the complex along with C8--X bond formation. However, this latter process is slow with bulky iodide and with neutral pyrrole, and in these cases moderately endothermic electron transfer (ca. 7 kcal mol(-1)) yielding the 8-quinolinyl radical occurs. Hydrogen exchange leads to a new radical on the 1-ethyl chain and to the observed products. These findings suggest that the mutagenic activity of the DNA-intercalated drug involves attack of the photogenerated cation to the heterocyclic bases.
Toward a Stackable CO2-to-CO Electrolyzer Cell Design─Impact of Media Flow Optimization (2023)

Aqueous CO2-to-CO electrolysis is a promising technology for closing the carbon cycle and defossilizing industrial processes. Considering the technological readiness, consensus has been achieved about using silver as a stable and selective electrocatalyst for the CO2-to-CO reduction reaction in aqueous electrolyte. On the other hand, challenges such as media flow management, component stability, and force distribution are still associated with improving the process performance and developing a stackable cell concept to meet industrially relevant levels. We therefore report on a promising stack concept with continuous flowcells operated with gas diffusion electrodes (GDEs). To enhance the CO2-to-CO conversion efficiency, dedicated media flow chambers were developed on two levels. In the gas chamber, which touches the GDE from the far side of the anode, the feed gas flow and distribution over the GDE were controlled by introducing various gas path architectures in a modular flowcell. In addition, an ionically conductive spacer was implemented in the catholyte chamber, which is adjacent to the opposite side of the GDE. The effect of these modifications on the cell voltage, selectivity, and overall conversion was investigated at 100 mA/cm2 with varying CO2 feed gas flow and concentration. Noteworthy, an optimized feed gas distribution generated an increase of the Faraday efficiency for CO under reduced CO2 supply. Furthermore, the implementation of the spacer enhanced the process stability by suppressing gas-bubble-induced noise in the cell voltage measurements. By functioning as support structures to the GDE, the combined modifications provided the cell with mechanical integrity and allowed an ionic and electric contact over the full active cell area, which is required for both stacking and upscaling of the cell. The corresponding performance was demonstrated by a two-cell short-stack.
Surface plasmons and Bloch surface waves: Towards optimized ultra-sensitive optical sensors (2017)

In photonics, the field concentration and enhancement have been major objectives for achieving size reduction and device integration. Plasmonics offers resonant field confinement and enhancement, but ultra-sharp optical resonances in all-dielectric multi-layer thin films are emerging as a powerful contestant. Thus, applications capitalizing upon stronger and sharper optical resonances and larger field enhancements could be faced with a choice for the superior platform. Here, we present a comparison between plasmonic and dielectric multi-layer thin films for their resonance merits. We show that the remarkable characteristics of the resonance behavior of optimized dielectric multi-layers can outweigh those of their metallic counterpart.
OSTEOID OSTEOMA OF THE SCAPULAR NECK: A CASE REPORT (2000)

Osteoid osteoma localised in the scapula are very rare. We report the case of an 11-year-old girl, who presented with an osteoid osteoma at the neck of the glenoid. This was excised en bloc after being located accurately by computed tomography. This case report demonstrates the difficulty in the approach to the excision of this unusually located lesion.
Expression of ion channels and receptors in Xenopus oocytes using vaccinia virus (1991)

The cytoplasmic injection of mRNA synthesized in vitro into Xenopus oocytes is widely used for heterologous expression of ion channels and neurotransmitter receptors. We report two new methods for expression of ion channels and receptors in oocytes using vaccinia virus (VV). 1) A recombinant VV carrying the Shaker H4 K+ channel cDNA driven by the VV P7.5 early promoter was injected into oocytes. 2) A recombinant VV containing the bacteriophage T7 RNA polymerase driven by the P7.5 promoter was coinjected along with plasmids containing a T7 promoter and cDNAs for channels and receptors. The functionally expressed proteins include a) voltage-gated ion channels: the Shaker H4 K+ channel and the rat brain IIA Na+ channel, b) a ligand-gated ion channel: the mouse muscle nicotinic acetylcholine receptor (AChR), and c) a G protein-coupled receptor: the rat brain 5HT1C receptor. After virus/cDNA injection into oocytes, these channels and receptors generally showed characteristics and expression levels similar to those observed in mRNA-injected oocytes. However, the AChR expressed at lower levels in virus/cDNA-injected oocytes than in mRNA-injected oocytes. Because our methods bypass mRNA synthesis, they are more rapid and convenient than the mRNA injection method. Potential applications to structure-function studies and expression cloning are discussed.
The State of the Art in Mobile Graphics Research (2008)

High-quality computer graphics let mobile-device users access more compelling content. Still, the devices' limitations and requirements differ substantially from those of a PC. This survey of mobile graphics research describes current solutions in terms of specialized hardware (including 3D displays), rendering and transmission, visualization, and user interfaces.