gebhart/patrepeval huggingface dataset:
Treatment of obstructive sleep apnea syndrome in children. (2000)

Obstructive sleep apnea (OSA) has been identified and recorded in paediatric patients, the potential mechanisms for OSA include anatomical abnormalities that lead to a narrowed airway space, reduced muscle tone and abnormal central ventilatory control. Several treatments have been developed and are routinely used to treat OSA in infants and children. Nasal mask continuous positive airway pressure (CPAP) is an effective non-invasive treatment that prevents the majority of obstructive events, reverses sleep disturbances, improves daytime performance and is associated with increased growth in patients with failure to thrive. Surgery to correct underlying anatomical abnormalities is frequently used and usually results in an improvement in symptoms and in some cases, it is curative. Other forms of treatment include pharmacological interventions, positioning and nasopharyngeal intubation.
Genetic organization of the mau gene cluster in Methylobacterium extorquens AM1: complete nucleotide sequence and generation and characteristics of mau mutants (1994)

The nucleotide sequence of the methylamine utilization (mau) gene region from Methylobacterium extorquens AM1 was determined. Open reading frames for 11 genes (mauFBEDACJGLMN) were found, all transcribed in the same orientation. The mauB, mauA, and mauC genes encode the periplasmic methylamine dehydrogenase (MADH) large and small subunit polypeptides and amicyanin, respectively. The products of mauD, mauG, mauL, and mauM were also predicted to be periplasmic. The products of mauF, mauE, and mauN were predicted to be membrane associated. The mauJ product is the only polypeptide encoded by the mau gene cluster which is predicted to be cytoplasmic. Computer analysis showed that the MauG polypeptide contains two putative heme binding sites and that the MauM and MauN polypeptides have four and two FeS cluster signatures, respectively. Mutants generated by insertions in mauF, mauB, mauE, mauD, mauA, mauG, and mauL were not able to grow on methylamine or any other primary amine as carbon sources, while a mutant generated from an insertion in mauC was not able to utilize methylamine as a source of carbon but utilized C2 to C4 n-alkylamines as carbon sources. Insertion mutations in mauJ, mauM, and mauN did not impair the ability of the mutants to utilize primary n-alkylamines as carbon sources. All mau mutants were able to utilize methylamine as a nitrogen source, implying the existence of an alternative (methyl)amine oxidation system, and a low activity of N-methylglutamate dehydrogenase was detected. The mauD, mauE, and mauF mutants were found to lack the MADH small subunit polypeptide and have a decreased amount of the MADH large subunit polypeptide. In the mauG and mauL mutants, the MADH large and small subunit polypeptides were present at wild-type levels, although the MADHs in these strains were not functional. In addition, MauG has sequence similarity to cytochrome c peroxidase from Pseudomonas sp. The mauA, mauD, and mauE genes from Paracoccus denitrificans and the mauD and mauG genes from Methylophilus methylotrophus W3A1 were able to complement corresponding mutants of M. extorquens AM1, confirming their functional equivalence. Comparison of amino acid sequences of polypeptides encoded by mau genes from M. extorquens AM1, P. denitrificans, and Thiobacillus versutus shows that they have considerable similarity.
An extraction method of positive blood cultures for direct identification ofCandidaspecies by Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry (2013)

Candida spp. are an important cause of nosocomial bloodstream infections. Currently, complete identification of yeasts with conventional methods takes several days. We report here the first evaluation of an extraction method associated with the Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry for direct identification of Candida species from positive blood cultures. We evaluated this protocol with blood cultures that were inoculated with reference and routine isolates (eight reference strains, 30 patients isolates and six mixed cultures containing two strains of different Candida species), or from patients with candidemia (28 isolates). This method performed extremely well (97% correct identification) with blood cultures of single Candida spp. and significantly reduced the time of diagnosis. Nevertheless, subculture remains indispensable to test fungal resistance and to detect mixed infections.
Design Features and Bruise Evaluation of an Apple Harvest and In-Field Presorting Machine (2018)

Abstract. In-field presorting of apples, in combination with the harvest aid function, would have advantages of cost savings in postharvest handling and storage, reduced postharvest pest and disease problems, and better inventory management, while also enhancing harvest productivity. A new apple harvest and in-field presorting prototype was developed to help apple growers achieve these potential benefits. The prototype sorts and grades fruit based on color and size, using a machine vision-based sorting system with an innovative fruit singulating and rotating design (SRD), and it handles the graded fruit in the bins using newly designed automatic bin fillers. Bruise damage by impact is a critical factor in the development of the apple harvest and in-field presorting prototype. This article reports on the major design features of the prototype and experimental evaluation of the prototype for potential bruise damage. Experiments were conducted on ‘Gala’ and ‘Fuji’ apples to evaluate bruise damage potential under both empty and partially filled bin conditions. An impact recording device (IRD) was used to measure the impact magnitude in terms of peak acceleration (G) at all critical points of the machine, including harvest conveyors, main conveyor, flat conveyor, SRD, cup conveyor, bin filler, and bins. It was found that bruise damage mainly occurred during bin filling. The number of impacts recorded for the partially filled bin was reduced by 60%, compared to that for the empty bin, indicating that the impact between apples and the wooden bin’s floor was a major cause of bruising. The maximum G value for the partially filled bin was measured at 34.5, while the measured G values were less than 20 from start to the point just before the bin filler, indicating no bruise damage. Bruise evaluation showed that no more than 9% of the test apples would be downgraded from ‘Extra Fancy’ grade for the partially filled bin condition. Higher G values for the empty bin condition suggested the need for further improvement to the discharge of apples from the bin filler to the bin to further reduce bruise damage. Keywords: Apples, Bruising, Fruit, Grading, Harvesting, Sorting, Machine vision.
Ceramic gas turbine engine demonstration program. Final report 2 Jan 76-31 May 81 (1982)

In view of endowing the surface of abutments, a component of titanium dental implant systems, with antioxidant and antimicrobial properties, a surface layer coated with epigallocatechin gallate (EGCg), a polyphenol belonging to the class of flavonoids, was built on titanium samples. To modulate interfacial properties, EGCg was linked either directly to the surface, or after populating the surface with terminally linked polyethyleneglycol (PEG) chains, Mw ~1600 Da. The underlying assumption is that fouling-resistant, highly hydrated PEG chains could reduce non-specific bioadhesion and magnify intrinsic EGCg properties. Treated surfaces were investigated by a panel of surface/interfacial sensitive techniques, to provide chemico-physical characterization of the surface layer and its interfacial environment. Results show: (i) successful EGCg coupling for both approaches; (ii) that both approaches endow the Ti surface with the same antioxidant properties; (iii) that PEG-EGCg coated surfaces are more hydrophilic and show a significantly higher (>50%) interaction force with water. Obtained results build up a rationale basis for evaluation of the merits of finely tuning interfacial properties of polyphenols coated surfaces in biological tests.
Charge transport in perovskite-type titanates: Space charge effects in ceramics and films (1994)

Abstract The role of space charge regions at grain boundaries and surfaces of acceptor-doped strontium titanate model materials is discussed and their effect on the defect chemistry, the charge transport processes, and the resistance degradation process is outlined. The behaviour of conventional ceramics and ceramic thin films is compared.
Characteristics of surgical prosthetic heart valves and problems around labelling: a document from the European Association for Cardio-Thoracic Surgery (EACTS)—The Society of Thoracic Surgeons (STS)—American Association for Thoracic Surgery (AATS) Valve Labelling Task Force (2019)

Intraoperative surgical prosthetic heart valve (SHV) choice is a key determinant of successful surgery and positive postoperative outcomes. Currently, many controversies exist around the sizing and labelling of SHVs rendering the comparison of different valves difficult. To explore solutions, an expert Valve Labelling Task Force was jointly initiated by the European Association for Cardio-Thoracic Surgery (EACTS), The Society of Thoracic Surgeons (STS) and the American Association for Thoracic Surgery (AATS). The EACTS-STS-AATS Valve Labelling Task Force, comprising cardiac surgeons, cardiologists, engineers, regulators and representatives from the International Organization for Standardization (ISO) and major valve manufacturers, held its first in-person meeting in February 2018 in Paris, France. This article was derived from the meeting's discussions. The Task Force identified the following areas for improvement and clarification: reporting of physical dimensions and characteristics of SHVs determining and labelling of SHV size, in vivo and in vitro testing and reporting of SHV haemodynamic performance and thrombogenicity. Furthermore, a thorough understanding of the regulatory background and the role of the applicable ISO standards, together with close cooperation between all stakeholders (including regulatory and standard-setting bodies), is necessary to improve the current situation. Cardiac surgeons should be provided with appropriate information to allow for optimal SHV choice. This first article from the EACTS-STS-AATS Valve Labelling Task Force summarizes the background of SHV sizing and labelling and identifies the most important elements where further standardization is necessary.
Growth and Characterization of Single-Crystal Multilayer Nanostructures for Fast Ion Conduction (2004)

Single-crystal multi-layered thin films of pure and mixed ceria and zirconia were heteroepitaxially grown using molecular beam epitaxy on yttria stabilized zirconia substrates. The growth of these films was monitored using in-situ reflection high-energy electron diffraction (RHEED). In addition, the ex-situ characterization techniques including X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM) and Rutherford backscattering spectrometry (RBS) along with ion channeling were used to further characterize these films.
Observations on surface-shoaling Cape hake off South West Africa (1974)

An unusual occurrence of surface-shoaling Cape hake, in association with extensive swarms of Nyctiphanes capensis, was located off South West Africa in November 1971 by a night-flying research aircraft, and sampled by a research purse-seiner. Synchronous airborne radiation thermometry and a hydrobiological cruise enabled the occurrence to be explained in terms of the vertical and horizontal distribution of dissolved oxygen content during upwelling conditions inducing a temporary midwater swimming habit in the fish, coupled with a fortuitous location of the euphausiid swarms. It is suggested that the fish followed the diurnal vertical migration of the euphausiids to the surface for feeding purposes.
Tetramethoxy‐äthylen, IX. Bildung und Reaktionen von 1.1.2.2‐Tetramethoxy‐cyclopropanen (1972)

Abstract Die Cyclopropanierung von Tetramethoxy‐äthylen ( 1 ) ergab nur geringe Mengen an 1.1.2.2‐Tetramethoxy‐cyclopropan ( 2 ). Dihalogencarbene addieren dagegen in guter Ausbeute an 1 zu den 1.1‐Dihalogen‐2.2.3.3‐tetramethoxy‐cyclopropanen ( 11 ), die thermisch leicht in die 2‐Halogen‐3.3‐dimethoxy‐acrylsäure‐ester ( 13 ) umgewandelt werden.
A novel enzyme-linked immunosorbent assay specific for high-molecular-weight adiponectin (2006)

Human plasma contains at least three forms of adiponectin: a trimer, a hexamer, and a high-molecular-weight (HMW) multimer. We purified HMW adiponectin from human plasma using its affinity to gelatin and obtained monoclonal antibodies against it. On Western blot analysis, the reactivity of these monoclonal antibodies was shown to be restricted to a non-heat-denatured form of adiponectin molecules. On heating, the collagen-like domain of adiponectin molecules became denatured, and thus the trimer form could not be maintained. From these, monoclonal antibodies against HMW adiponectin were suggested to react with the intact trimer of adiponectin. With these monoclonal antibodies, we developed a sandwich ELISA system for quantifying adiponectin in human serum. Its specificity was verified by analysis of serum fractions separated by gel-filtration chromatography, and our ELISA system was found to be HMW adiponectin-specific. With this novel ELISA, the HMW adiponectin concentrations were 8.4 +/- 5.5 microg/ml (mean +/- SD) in healthy women and 6.2 +/- 3.6 microg/ml in healthy men. Also, serum with a lower HMW adiponectin concentration was shown to have a lower HMW ratio (i.e., HMW adiponectin/total adiponectin).
Comparison of the efficacy of cardamom (Elettaria cardamomum) with pioglitazone on dexamethasone-induced hepatic steatosis, dyslipidemia, and hyperglycemia in albino rats (2015)

To evaluate the efficacy of cardamom with pioglitazone on dexamethasone-induced hepatic steatosis, dyslipidemia, and hyperglycemia in albino rats. There were four groups of 6 rats each. First group received dexamethasone alone in a dose of 8 mg/kg intraperitoneally for 6 days to induce metabolic changes and considered as dexamethasone control. Second group received cardamom suspension 1 g/kg/10 mL of 2% gum acacia orally 6 days before dexamethasone and 6 days during dexamethasone administration. Third group received pioglitazone 45 mg/kg orally 6 days before dexamethasone and 6 days during dexamethasone administration. Fourth group did not receive any medication and was considered as normal control. Fasting blood sugar, lipid profile, blood sugar 2 h after glucose load, liver weight, liver volume were recorded, and histopathological analysis was done. The effects of cardamom were compared with that of pioglitazone. Dexamethasone caused hepatomegaly, dyslipidemia and hyperglycemia. Both pioglitazone and cardamom significantly reduced hepatomegaly, dyslipidemia, and hyperglycemia (P < 0.01). Reduction of blood sugar levels after glucose load was significant with pioglitazone in comparison to cardamom (P < 0.01). Cardamom has comparable efficacy to pioglitazone in preventing dexamethasone-induced hepatomegaly, dyslipidemia, and fasting hyperglycemia.
Simultaneous measurement of size and velocity of small particles in laminar flows (1996)

A new laser anemometer gauge is presented here. The velocity and the size of spherical particles are simultaneously measured without uniform illumination and preliminary calibration. The used method is based on Mie’s scattering light theory. The device is able to measure the velocity and size of hydrogen bubbles electrolytically produced by a platinum wire immersed in the water flow.
A newly developed upper limb single-joint HAL in a patient with elbow flexion reconstruction after traumatic brachial plexus injury: A case report (2017)

We report a case in which elbow flexion exercises using the upper limb single-joint Hybrid Assistive Limb (upper limb HAL-SJ) were implemented in a patient 13 months postoperatively following elbow flexion reconstruction with intercostal nerve transfer after a traumatic brachial plexus injury. Treatment using the upper limb HAL-SJ was administered once a week for 10 sessions from 13 to 16 months after surgery. Exercises using the upper limb HAL-SJ supported elbow motion by detecting bioelectric signals through surface electrodes on the biceps and triceps brachii. No adverse events were observed during treatment with the upper limb HAL-SJ. Improvements in elbow flexion strength were observed during treatment. Treatment with the upper limb HAL-SJ can be performed safely and effectively following elbow flexion reconstruction by intercostal nerve transfer after a traumatic brachial plexus injury.
The rod transducin alpha subunit amino terminus is heterogeneously fatty acylated. (1992)

Rod transducin (Tr), a heterotrimeric GTP-binding protein composed of alpha, beta, and gamma subunits, couples photolysis of rhodopsin to the activation of cyclic GMP phosphodiesterase in the vertebrate visual signal transduction cascade. To determine if T alpha r is covalently modified, we analyzed tryptic fragments of bovine retinal T alpha r using electrospray mass spectrometry, liquid chromatography/mass spectrometry, tandem mass spectrometry, and gas chromatography. A novel heterogeneous fatty acylation was detected at the NH2 terminus. Four types of NH2-terminal tryptic fragments of T alpha r were isolated, and each contained either a lauroyl (C12:0), myristoyl (C14:0), (cis-delta 5)-tetradecaenoyl (C14:1) or (cis,cis-delta 5, delta 8)-tetradecadienoyl (C14:2) fatty acyl residue amide-linked to the NH2-terminal glycine residue. NH2-terminal fatty acylation does not anchor T alpha r permanently in the membrane, since T alpha r used in these experiments was eluted without detergent from rod outer segment membranes.
Comparison of Fe2O3and Fe2CoO4core-shell plasmonic nanoparticles for aptamer mediated SERS assays (2016)

Conjugation of oligonucleotides or aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound bundles of nanoparticles which cause a measurable change in the colloid’s optical properties. Here, we present further optimization of a “SERS off” competitive binding assay utilizing plasmonic and magnetic nanoparticles for the detection of the toxin bisphenol A (BPA). The assay involves 1) a ‘target’ silver nanoparticle functionalized with a Raman reporter dye and PEGylated BPA-binding DNA aptamers, and 2) a version of the toxin BPA, bisphenol A diglycidyl ether (BADGE), PEGylated and immobilized onto a silver coated magnetic ’probe’ nanoparticle. When mixed, these target and probe nanoparticles cluster into magnetic dimers and trimers and an enhancement in their SERS spectra is observed. Upon introduction of free BPA in its native form, target AgNPs are competitively freed; reversing the nanoparticle assembly and causing the SERS signal to “turn-off” and decrease in response to the competitive binding event. The assay particles were housed inside two types of optofluidic chips containing magnetically active nickel pads, in either a straight or spotted pattern, and both Fe<sub>2</sub>O<sub>3</sub> and Fe<sub>2</sub>CoO<sub>4</sub> were compared as magnetic cores for the silver coated probe nanoparticle. We found that the Ag@ Fe<sub>2</sub>O<sub>3</sub> particles were, on average, more uniform in size and more stable than Ag@ Fe<sub>2</sub>CoO<sub>4</sub>, while the addition of cobalt significantly improved the collection time of particles within the magnetic chips. Using 3D Raman mapping, we found that the straight channel design with the Ag@ Fe<sub>2</sub>O<sub>3</sub> particles provided the most uniform nanoparticle organization, while the spotted channel design with Ag@ Fe<sub>2</sub>CoO<sub>4</sub> demonstrated a larger SERS enhancement, and thus a lower limit of detection.
Data from Guidelines for the Evaluation of Immune Therapy Activity in Solid Tumors: Immune-Related Response Criteria (2023)

&lt;div&gt;Abstract&lt;p&gt;&lt;b&gt;Purpose:&lt;/b&gt; Immunotherapeutic agents produce antitumor effects by inducing cancer-specific immune responses or by modifying native immune processes. Resulting clinical response patterns extend beyond those of cytotoxic agents and can manifest after an initial increase in tumor burden or the appearance of new lesions (progressive disease). Response Evaluation Criteria in Solid Tumors or WHO criteria, designed to detect early effects of cytotoxic agents, may not provide a complete assessment of immunotherapeutic agents. Novel criteria for the evaluation of antitumor responses with immunotherapeutic agents are required.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Experimental Design:&lt;/b&gt; The phase II clinical trial program with ipilimumab, an antibody that blocks CTL antigen-4, represents the most comprehensive data set available to date for an immunotherapeutic agent. Novel immune therapy response criteria proposed, based on the shared experience from community workshops and several investigators, were evaluated using data from ipilimumab phase II clinical trials in patients with advanced melanoma.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results:&lt;/b&gt; Ipilimumab monotherapy resulted in four distinct response patterns: (&lt;i&gt;a&lt;/i&gt;) shrinkage in baseline lesions, without new lesions; (&lt;i&gt;b&lt;/i&gt;) durable stable disease (in some patients followed by a slow, steady decline in total tumor burden); (&lt;i&gt;c&lt;/i&gt;) response after an increase in total tumor burden; and (&lt;i&gt;d&lt;/i&gt;) response in the presence of new lesions. All patterns were associated with favorable survival.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Conclusion:&lt;/b&gt; Systematic criteria, designated immune-related response criteria, were defined in an attempt to capture additional response patterns observed with immune therapy in advanced melanoma beyond those described by Response Evaluation Criteria in Solid Tumors or WHO criteria. Further prospective evaluations of the immune-related response criteria, particularly their association with overall survival, are warranted. (Clin Cancer Res 2009;15(23):7412–20)&lt;/p&gt;&lt;/div&gt;
Regulation of Dendritic Cell Function with Immunomodulatory Drugs (2005)

Dendritic cells (DC) represent a sentinel-like system with the capacity to capture and process antigens, to migrate into secondary lymphoid organs, and to activate naive T lymphocytes. DCs are considered as important elements in the induction of specific immune responses. The potency of DCs for induction of immune responses can be affected by a number of aspects related to their maturation stage and state of activation. For a long time, the critical role of DCs in many clinical situations was underscored. DCs in vivo might represent a favored target for immune tolerance (cancer) or immune activation (auto-immune diseases and allergic hypersensitivity). For immunotherapeutic applications, it is crucial to identify factors and agents that might affect the differentiation, maturation and function of DCs. This review will focus on the impact of the most widely used immunomodulatory drugs in the clinical setting on the properties and functions of DCs. The modulation and manipulation of DC functions through exposure of DCs to immunomodulatory agents both in vitro and in vivo is an approach that could result in therapeutic benefit in many clinical situations. Keywords: dendritic cells, immunomodulation, tolerance, immunostimulation, cytokines