gebhart/patrepeval huggingface dataset:
Protein turnover in lipid homeostasis (2018)

Lipid droplet (LD) is emerging as a hotbed of metabolic activity as opposed to previously held notion of an `inert` body. The life cycle of a lipid droplet is largely regulated by its resident proteins most of which are turned over by the cellular degradation machineries. To date, two prominent protein degradation machineries have been identified, the ubiquitin proteasome system (UPS) and autophagy. The proteasome machinery turns over short lived proteins while bulk degradation of long lived proteins is usually associated with autophagy. The breakdown of stored lipid at the LD by sequestration of the autophagy machinery is termed lipophagy, a process in which lipids are broken down in acidic lysosomal compartments as opposed to the classical cytosolic breakdown at neutral pH by TG lipases. This review presents the different players fine tuning the proteasomal and autophagic degradation systems in regulating lipid metabolism. Key words: lipid droplets, proteasome, ubiquitin, E3 ligases, triacylglycerol, cholesterol, lipophagy, Rab GTPases
Use of lacZ Fusions to Delimit Regulatory Elements of the Inducible Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae (1984)

We present the DNA sequence of a 914-base pair fragment from Saccharomyces cerevisiae that contains the GAL1-GAL10 divergent promoter, 140 base pairs of GAL10 coding sequence, and 87 base pairs of GAL1 coding sequence. From this fragment, we constructed four pairs of GAL1-lacZ and GAL10-lacZ fusions on various types of yeast plasmid vectors. On each type of vector, the fused genes were induced by galactose and repressed by glucose. The response of a GAL1-lacZ fusion to gal4 and gal80 regulatory mutations was similar to the response of intact chromosomal GAL1 and GAL10 genes. A set of deletions that removed various portions of the GAL10 regulatory sequences from a GAL10-CYC1-lacZ fusion was constructed in vitro. These deletions defined a relatively guanine-cytosine-rich region of 45 base pairs that contained sequences necessary for full-strength galactose induction and an adjacent guanine-cytosine rich 55 base pairs that contained sequences sufficient for weak induction.
DNA Sequences That Interfere with Transcription: Implications for Genome Function and Stability (2013)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTDNA Sequences That Interfere with Transcription: Implications for Genome Function and StabilityBoris P. Belotserkovskii†, Sergei M. Mirkin‡, and Philip C. Hanawalt*†View Author Information† Department of Biology, Stanford University, Stanford, California 94305, United States‡ Department of Biology, Tufts University, Medford, Massachusetts 02155, United States*E-mail: [email protected]Cite this: Chem. Rev. 2013, 113, 11, 8620–8637Publication Date (Web):August 23, 2013Publication History Received6 February 2013Published online23 August 2013Published inissue 13 November 2013https://doi.org/10.1021/cr400078yCopyright © 2013 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views2386Altmetric-Citations84LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (7 MB) Get e-AlertscloseSUBJECTS:Chemical structure,DNA replication,Genetics,Nucleic acid structure,Peptides and proteins Get e-Alerts
Clinical and statistical evaluation of self-monitoring blood glucose meters. (1998)

Both the Spearman's and Wilcoxon's paired tests, although commonly used, are inappropriate to evaluate SMBG systems. The percentage of SMBG values within the +/-10% interval and the error grid analysis are more accurate, because they consistently classified the five glucose meters tested in our study with a high degree of reproducibility.
Treatment of idiopathic scoliosis in the Milwaukee brace. (1980)

The results of treatment of idiopathic scoliosis with a Milwaukee brace were studied in 133 patients (127 girls and six boys) whose ages ranged from eight years and five months to sixteen years and two months at the beginning of treatment. These 133 patients had 192 separate curves (119 right thoracic, thirty-nine high lumbar, twelve thoracolumbar, and twenty-two high left thoracic). Of these patients, seventy-four with 109 curves were followed for five years or more after the brace was discontinued (average, eight years; range, five to thirteen years); twenty-nine patients were treated surgically because of a poor response to the brace or progression of the curve; and thirty patients were lost to follow-up. More than 80 per cent of the seventy-four patients followed for five years or more showed some increase of their curves after the brace was discontinued. The average correction at follow-up compared with the original curve was 2 degrees for thoracic curves (range, -18 to 24 degrees) and 4 degrees for the thoracolumbar and lumbar curves (range, -11 to 17 degrees). The brace was more effective for curves of less than 40 degrees. More than one-third of the patients with curves of 40 degrees or more eventually required surgical treatment. Age, curve pattern, and status of the iliac and ring epiphyses did not correlate withe response to brace treatment. The best guideline for prediction of the results of brace treatment was the response of the curve to the brace, especially during the first year of treatment. If the curve is reduced in the brace to less than 50 per cent of its initial measurement, there is a good chance of obtaining significant permanent correction.
Levels of detection of tumor cells in human bone marrow with or without prior culture. (1990)

With an in vitro culture technique combined with light microscopy, immunocytochemistry and molecular probing, we previously detected occult tumor cells in histologically-normal human bone marrow harvested for autologous transplantation. In this study, we mixed known numbers of malignant lymphoid (Raji and CEM) or breast cancer (MCF-7) cells with normal human bone marrow cells to determine the levels at which tumor cells can be detected before and after culture. Cytocentrifuge preparations were made before culture and after 2 or more weeks of culture and examined by light microscopy. We detected contaminating lymphoma cells at a level of more than 5% before culture, and at a level of 0.01% after culture for 2 or more weeks in 2% human lymphocyte conditioned medium. Before culture, we detected MCF-7 cells at a level of 0.001% using glucose oxidase immunocytochemical staining techniques; these cells were detected at a level of 0.00001% after culture. Since, of necessity, these calibrations rations were performed using cell lines, it is likely that these results overestimate the absolute sensitivity of these methods for detection of tumor cells in patient samples. We found the glucose oxidase immunocytochemical method more specific for detecting occult tumor cells in bone marrow than the immunoperoxidase staining method because of the absence of non-specific staining arising from endogenous peroxidase in bone marrow cells which makes the interpretation of the latter difficult. We conclude that culture techniques can increase the sensitivity of detection of occult tumor cells in human bone marrow about 100-fold.
HIV Env-Specific IgG Antibodies Induced by Vaccination of Neonatal Rhesus Macaques Persist and Can Be Augmented by a Late Booster Immunization in Infancy (2020)

The HIV epidemics in infants and adolescent women are linked. Young women of childbearing age are at high risk for HIV infection and, due to poor HIV testing rates and low adherence to antiretroviral therapy, are at high risk for mother-to-infant transmission. We hypothesize that HIV vaccine regimens initiated in early life would provide the necessary time frame to induce mature and highly functional Env-specific antibody responses that could potentially also protect against HIV acquisition later in life. The present study was designed to test two vaccine regimens, a clade C HIV Env protein vaccine (Env only) alone or combined with a modified vaccinia Ankara (MVA) vector expressing HIV Env (MVA/Env) for the induction and persistence of Env-specific antibody responses in an infant nonhuman primate model. Vaccination was initiated within the first week of life, with booster immunizations at weeks 6, 12, and 32. We demonstrate that both vaccine strategies were able to elicit durable Env-specific antibody responses that were enhanced by a late boost in infancy. Furthermore, we confirmed earlier data that intramuscular administration of the Env protein with the Toll-like receptor 7/8 (TLR7/8)-based adjuvant 3M-052 in stable emulsion (3M-052-SE) induced higher Env-specific antibody responses than vaccination with Env adjuvanted in Span85-Tween 80-squalene (STS) tested in a previous study. These results support the concept of early vaccination as a means to induce durable immune responses that may prevent HIV infection in adolescence at the onset of sexual debut.<b>IMPORTANCE</b> The majority of new HIV-1 infections occur in young adults, with adolescent women being 3 times more likely to acquire HIV than young men. Implementation of HIV prevention strategies has been less successful in this age group; thus, a vaccine given prior to adolescence remains a high priority. We propose that instead of starting HIV vaccination during adolescence, an HIV vaccine regimen initiated in early infancy, aligned with the well-accepted pediatric vaccine schedule and followed with booster immunizations, will provide an alternative means to reduce HIV acquisition in adolescence. Importantly, the long window of time between the first infant vaccine dose and the adolescence vaccine dose will allow for the maturation of highly functional HIV Env-specific antibody responses. Our study provides evidence that early life vaccination induces durable Env-specific plasma IgG responses that can be boosted to further improve the quality of the antibody response.
Paramagnetic Resonance of Some Carborane Analogs of Ferricenium Cation (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTParamagnetic Resonance of Some Carborane Analogs of Ferricenium CationAugust H. Maki and Thomas E. BerryCite this: J. Am. Chem. Soc. 1965, 87, 20, 4437–4441Publication Date (Print):October 1, 1965Publication History Published online1 May 2002Published inissue 1 October 1965https://pubs.acs.org/doi/10.1021/ja00948a005https://doi.org/10.1021/ja00948a005research-articleACS PublicationsRequest reuse permissionsArticle Views192Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A 50 Gb/s 32×32 CMOS crossbar chip using asymmetric serial links (2003)

A 32/spl times/32 synchronous crossbar chip was designed in a 0.27 /spl mu/m CMOS technology for use in a high-speed network switch. The crossbar chip uses 32 Asymmetric Serial Links to achieve high speed at the interfaces and to reduce both power and area. The crossbar switch core is implemented with static CMOS multi-stage multiplexers with multicast capability. The chip operates successfully with links running at 1.6 Gb/s. The measured bit-error-rate is <10/sup -14/ when all channels and the switch core are operating. The crossbar chip consumes 5 W and provides a total bandwidth above 50 Gb/s.
Synthesen in der Polymyxinreihe. 12. Mitteilung. Die Synthese von Polymyxin D1 (1970)

Abstract The synthesis of the cycloheptapeptide XI (Figure 1), corresponding to the structure proposed by T. Suzuki et al , for the antibiotic Polymyxin D 1 is reported. This synthetic compound proved to be identical in all respects with natural Polymyxin D 1 .
Large thermoelectric power inNaCo2O4single crystals (1997)

We measured and analyzed the transport properties of single-crystal ${\mathrm{NaCo}}_{2}{\mathrm{O}}_{4}$, which is a metallic transition-metal oxide consisting of a two-dimensional triangle lattice of Co. Reflecting the crystal structure, the resistivity is highly anisotropic between in- and out-of-plane directions, and the in-plane resistivity is as low as 200 $\ensuremath{\mu}\ensuremath{\Omega}$ cm at 300 K. Most strikingly, the in-plane thermoelectric power of ${\mathrm{NaCo}}_{2}{\mathrm{O}}_{4}$ is about 100 $\ensuremath{\mu}$V/K at 300 K, which is nearly ten times larger than that of typical metals. The large thermoelectric power and the low resistivity suggest that ${\mathrm{NaCo}}_{2}{\mathrm{O}}_{4}$ is a potential thermoelectric material.
Solving the Puzzle of the September 2018 Palu, Indonesia, Tsunami Mystery: Clues from the Tsunami Waveform and the Initial Field Survey Data (2018)

On September 28, 2018, following a magnitude 7.5 strike-slip fault earthquake, an unexpected tsunami inundated the coast of Palu bay, Sulawesi, Indonesia, causing many casualties and extensive property damage. However, the earthquake’s mechanism rarely generates a destructive tsunami. The tidal record at Pantoloan, located along the coast of Palu bay, indicates that the tsunami arrived 6 min after the earthquake and generated 2 m of receding water. It had a maximum wave height of 2 m and arrived approximately 2 min later. The tsunami had a relatively short period and caused devastation as far inland as 300 m. Additionally, 8 m high watermarks were observed near the coast; the flow depth decreased to 3.5 m inland (Fig. 1). Amateur videos and eyewitness accounts indicate that the tsunami did not enter the bay through its mouth but obliquely from an area inside the bay. Our hypothesis, therefore, is that the killer tsunami was most likely generated by an underwater landslide occurring inside Palu bay. While detailed bathymetric data are still needed to confirm this hypothesis, in this article we provide a preliminary analysis of the available data, supported by the results of a field survey, to strengthen this hypothesis and provide direction for further post-tsunami surveys and analysis.
The differential diagnosis of recurrent episodes of vertigo (2016)

Neurovestibularexamnation using the protocol ALGORITM is important for assessment of the level of vestibular system lesion. Iitis necessary to use it in complex examination of patients with complaints of vertigo.
In vitro stability of osteocalcin (1994)

Journal Article In vitro stability of osteocalcin Get access G Banfi, G Banfi Dipartimento di Med. di Lab., Istituto Scientifico H. San Raffaele, Milano, Italy Search for other works by this author on: Oxford Academic Google Scholar R Daverio R Daverio Dipartimento di Med. di Lab., Istituto Scientifico H. San Raffaele, Milano, Italy Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 40, Issue 5, 1 May 1994, Pages 833–834, https://doi.org/10.1093/clinchem/40.5.833a Published: 01 May 1994
Surgery of thoracoabdominal aneurysms (1989)

Seventeen patients with thoracoabdominal aneurysms, including 5 ruptured aneurysms, were operated upon using a left diaphragm-splitting thoracoabdominal incision and the retroperitoneal route. A temporary shunt was used in 13 patients, femorofemoral perfusion in 1 and cold perfusion cooling of the kidneys in 3 patients. The step-by-step reattachment technique into ready-made limbs or holes in the Dacron graft ensured that visceral and renal ischaemic times remained within acceptable limits. The mean renal and proximal clamping times were 44 and 77 min, respectively. One patient with a ruptured aneurysm (6%) died of diffuse bleeding. The others recovered without paraplegic, renal or other severe complications. During the follow-up period, mean 44 months and range 10-116 months, 3 patients died of lung cancer and 2 of coronary disease giving a late mortality of 29%. The remaining 11 patients are alive and well. The cumulative 2- and 5-year survival is 87% and 62% respectively. The patency rate of the grafts was 100% and that of the 30 individually revascularised arteries 80%. We recommend elective surgery for thoracoabdominal aneurysms using a temporary shunt or cold perfusion cooling of the kidneys as a protective measure against perioperative ischaemia.
Wavelet bispectral analysis for the study of interactions among oscillators whose basic frequencies are significantly time variable (2007)

Bispectral analysis, recently introduced as a technique for revealing time-phase relationships, is extended to make use of wavelets rather than Fourier analysis. It is thus able to encompass instantaneous phase-time dependence for the case of two or more coupled nonlinear oscillators. The method is demonstrated and evaluated by use of test signals from a pair of coupled Poincaré oscillators. It promises to be useful in a wide range of scientific contexts for studies of interacting oscillators whose basic frequencies are significantly time variable.
Rhodium nitrosyl catalysts for CO2 hydrogenation to formic acid under mild conditions (2012)

A new synthetic protocol for catalysing CO 2 hydrogenation to formic acid under mild conditions is reported, and the CO 2 hydrogenation is efficiently achieved by dcpe‐rhodium‐nitrosyl catalyst precursors, Rh(NO)(dcpe) (1) (dcpe = 1,2‐dicyclohexylphosphinoethane) and Rh(III)(NO)(dcpe)Cl 2 (2). The catalytic activity of 1 is noteworthy for being able to proceed in the absence of protic conditions. Compound 2 is characterized by NMR, IR and X‐ray crystallography. In particular, 2 is observed to bear a bent NO ligand with a Rh–N–O angle of 115.7(3)°, representing one of the smallest M–N–O angles known. Copyright © 2012 John Wiley &amp; Sons, Ltd.
Delay of Senescence in Carnations by a Rhizobitoxine Analog and Sodium Benzoate1 (1977)

Abstract The rhizobitoxine analog, L-2-amino-4-(2-aminoethoxy)- trans -3-butenoic acid, and sodium benzoate inhibited ethylene production in cut flowers of carnation ( Dianthus caryophyllus , L. cv. White Sim) and extended the vase life by several days. The rhizobitoxine analog (Ro) and sodium benzoate were added to a basal holding solution of 2% sucrose, 0.02% 8-hydroxyquinoline citrate, and 0.02 M potassium citrate buffer (pH 4.7). The results indicate that Ro at 0.068 mM increased the vase life of cut carnations by 95% or more. Sodium benzoate at 1.0 or 2.0 mM also increased the vase life. The compounds may have increased vase life of the flowers by inhibiting ethylene production. The effects of these compounds were over and above the effects of sucrose, 8-hydroxyquinoline citrate, and acid pH of the holding solution.
The complete coordination chemistry - one practioner's perspective (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe complete coordination chemistry - one practioner's perspectiveDaryle H. BuschCite this: Chem. Rev. 1993, 93, 3, 847–860Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/cr00019a001https://doi.org/10.1021/cr00019a001research-articleACS PublicationsRequest reuse permissionsArticle Views1462Altmetric-Citations146LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Patchy Occurrence of Cowpox Virus in Voles from Germany (2020)

Cowpox virus (CPXV), genus <i>Orthopoxvirus</i>, family <i>Poxviridae</i>, is a zoonotic pathogen in Eurasian wild rodents. High seroprevalences have been reported previously for vole and murine species in Europe. In contrast, viral DNA was only rarely detected, and very few reservoir-derived CPXV isolates exist. In this study, CPXV DNA and CPXV-reactive antibodies were monitored in wild small mammals for 5 years in four German federal states. Screening of liver tissues of 3966 animals by CPXV real-time PCR (qPCR) revealed five voles of two species positive for CPXV DNA. Two positive bank voles (<i>Myodes glareolus</i>) and two positive common voles (<i>Microtus arvalis</i>) originated from two plots in Baden-Wuerttemberg. One positive bank vole originated from Mecklenburg-Western Pomerania. None of the small mammals from Thuringia and North Rhine-Westphalia was positive in the qPCR. CPXV antigen-based indirect immunofluorescence assays of 654 highly diluted chest cavity fluid samples detected two bank voles and two common voles from the same sites in Baden-Wuerttemberg to be highly seroreactive. Five animals were CPXV DNA positive, and four other animals were orthopoxvirus seropositive. Our study indicates both a very low prevalence and a patchy occurrence of CPXV in common and bank voles and absence in other rodent and shrew species in Germany. The multiple detection of infected voles at one site in Baden-Wuerttemberg and continued detection in a region of Mecklenburg-Western Pomerania classify these regions as potential endemic foci.
Impact of Research and Development, Analysis, and Standardization on PV Project Financing Costs (2018)

The technical report discusses how R and D efforts focused on removing perceived risk from cash flows to investors have the potential to lower the cost of capital and increase the amount of leverage in a solar project. It also discusses how creating business efficiencies that allow financing transactions to occur more quickly with less effort can reduce the upfront costs associated with arranging financing for a solar project or group of projects. The paper then assesses the impact that these R and D activities might have on the volatility of PV asset cash flows and asset value, as well as the upfront costs of arranging a financial transaction. Finally, we insert these assumptions into financial models to analyze their impacts on the cost of capital for equity and debt investors, project leverage, and upfront financial transaction costs.