gebhart/patrepeval huggingface dataset:
A mutation in the Rho1-GAP-encoding gene BEM2 of Saccharomyces cerevisiae affects morphogenesis and cell wall functionality (1998)

Saccharomyces cerevisiae strain V918 was previously isolated in a search for thermosensitive autolytic mutants and found to bear a recessive mutation that caused the development of multinucleate swollen cells undergoing cell lysis. The BEM2 gene has been isolated by complementation of the phenotype of a V918 segregant. BEM2 encodes a Rho-GTPase-activating protein (GAP) which is thought to act as a modulator of the Rho1 small GTPase. It is shown that the mutation causing the morphogenetic and autolytic phenotype in strain V918 and its segregants lies in the BEM2 gene, defining a new mutant allele, bem2-21. Mutants in the BEM2 gene have been reported to display loss of cell polarity and depolarization of the actin cytoskeleton, causing a bud-emergence defect. Low resistance to sonication and to hydrolytic enzymes proved that the cell wall is less protective in bem2-21 mutants than in wild-type strains. Moreover, bem2-21 mutants are more sensitive than the wild-type to several antifungal drugs. Transmission electron microscopy revealed the development of abnormally thick and wide septa and the existence of thin areas in the cell wall which probably account for cell lysis. The depolarization of actin in bem2-21 mutants did not preclude morphogenetic events such as cell elongation in homozygous diploid strains during nitrogen starvation in solid media, hyperpolarization of growth in a background bearing a mutated septin, or sporulation. Multinucleate cells from bem2-21 homozygous diploids underwent sporulation giving rise to multispored asci ('polyads'), containing up to 36 spores. This phenomenon occurred only under osmotically stabilized conditions, suggesting that the integrity of the ascus wall is impaired in cells expressing the bem2-21 mutation. It is concluded that the function of the BEM2 gene product is essential for the maintenance of a functional cell wall.
Low levels of soluble receptor for advanced glycation end products in non-ST elevation myocardial infarction patients (2009)

Serum levels of sRAGE were low in NSTEMI patients, and were negatively correlated with extent of lesion, inflammatory mediators, AGEs and AGEs/sRAGE. Both sRAGE and AGEs/sRAGE may serve as biomarkers/predictors for identifying NSTEMI patients.
87 EXPERIMENTAL EVIDENCE FOR THE FORMATION OF A COMPLEX BETWEEN BUTYRYL COENZYME A DEHYDROGENASE AND THE ‘ELECTRON TRANSFERRING FLAVOPROTEIN’, ETF (1998)

Conference Article| February 01 1998 87 EXPERIMENTAL EVIDENCE FOR THE FORMATION OF A COMPLEX BETWEEN BUTYRYL COENZYME A DEHYDROGENASE AND THE ‘ELECTRON TRANSFERRING FLAVOPROTEIN’, ETF ANTONY PARKER; ANTONY PARKER 1Department of Biochemistry, UCD, Belfield, Dublin 4, Eire Search for other works by this author on: This Site PubMed Google Scholar PAUL C. ENGEL PAUL C. ENGEL 1Department of Biochemistry, UCD, Belfield, Dublin 4, Eire Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1998) 26 (1): S73. https://doi.org/10.1042/bst026s073 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation ANTONY PARKER, PAUL C. ENGEL; 87 EXPERIMENTAL EVIDENCE FOR THE FORMATION OF A COMPLEX BETWEEN BUTYRYL COENZYME A DEHYDROGENASE AND THE ‘ELECTRON TRANSFERRING FLAVOPROTEIN’, ETF. Biochem Soc Trans 1 February 1998; 26 (1): S73. doi: https://doi.org/10.1042/bst026s073 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1998 Biochemical Society1998 Article PDF first page preview Close Modal You do not currently have access to this content.
Modal refractive index measurement in nanowire lasers—a correlative approach (2018)

We present a method to correlate multimodal measurements—namely optical spectroscopy and electron microscopy—over large ensembles of randomly distributed single nano-objects. Using an algorithmic approach derived from astrometry, a marker-free method of uniquely associating nano-objects characterised using multiple techniques is described. This approach is applied to nanolasers, enabling an experimental calculation of modal refractive index in sub-micron diameter nanowires. By matching the lasing spectrum and electron microscopy image of 13 nanowire lasers, the refractive index of the TE01 mode in GaAs/AlGaAs multiple-quantum well nanolasers is determined to be n g = 4.7 ± 0.3.
Colloidal Stability of Silk Fibroin Nanoparticles Coated with Cationic Polymer for Effective Drug Delivery (2015)

Generally, silk fibroin nanoparticles (SFNPs) are great candidates to deliver drugs or other bioactive substances in vivo. However, their further applications are largely limited by the low colloidal stability of SFNPs, as they tend to aggregate in biological media. To address this issue, SFNP composite materials with a core-shell structure (CS-SFNPs) were fabricated by coating SFNPs with four different selected cationic polymers, glycol chitosan, N,N,N-trimethyl chitosan, polyethylenimine, and PEGylated polyethylenimine, through electrostatic interaction. According to the DLS and NTA results, compared with the bare SFNPs, the CS-SFNPs showed much higher colloidal stability in biological media. When treated with human cervical carcinoma (HeLa) cells, the CS-SFNPs were efficiently internalized and accumulated in lysosome; and when loaded with an anticancer drug, DOX, the CS-SFNPs also showed higher cytotoxicity against HeLa cells. Our results suggest that the fabricated CS-SFNPs with desirable colloidal stability in biological media have the potential to be employed as drug carriers for the anticancer drug delivery system.
A secure and practical key management mechanism for NFC read-write mode (2011)

Near Field Communication (NFC) is a short-range communication technology and has been applied around the world. The most common service of NFC will be micropayments. Technically, the card emulation mode of NFC will simulate mobile devices such as cell phones into stored value cards, and then debit from external readers. Apart from simulating cell phones as stored value cards, read-write mode of NFC enables the ability of devices to read and write external cards. In the context of micropayments, cell phone can be as the POS devices to read the balances from external cards and perform the actions of debiting or storing value. In this application condition, it is important to store keys of external cards securely when reading and writing. First, we discuss the security issues of key storage of NFC devices as reading and writing external card, analyze the possible risk of every solution. Last, we propose a secure and practical NFC key management mechanism and apply it on contactless mobile debit. According to the implementing results, the mechanism performs well in the efficacy and the user satisfaction.
Hematopoietic Differentiation of Human Embryonic Stem Cells in Vitro : Comparison of Three Cell Lines (2008)

Abstract Human embryonic stem cells (hES) isolated from the inner cell mass of a blastocyst have the ability to self renew indefinitely while maintaining their pluripotency to differentiate into multiple cell lineages. Therefore, hES represent an important source of cells for perspective cell therapies and serve as an essential tool for fundamental research, specifically for understanding pathophysiological mechanisms of human diseases for the development of novel pharmacological drugs. The generation of hematopoietic stem cells from hES may serve as an alternative source of cells for hematopoietic reconstitution following bone marrow transplantation and an interesting approach to understand early stages of hematopoietic development which are difficult to study in human embryos. Using two different methods, we have differentiated three hES cell lines (SA01, H1 and H9) into hematopoietic cells by generating embryoid bodies and co-culturing on the murine Op9 cell line. In both experimental approaches, we obtain cells expressing CD34 and when cultured in hematopoietic conditions, SA01 and H1 cell lines differentiate into various hematopoietic lineages as demonstrated by BFU-E, CFU-GM and CFU-GEMM colony formation, whereas H9 have almost exclusively granulo-macrophage differentiation. Cells composing these hematopoietic colonies express CD45, CD11b, CD31, CD41 and CD235 and staining with May Grundwald-Giemsa demonstrate neutrophil and erythrocyte morphology. These results demonstrate the capacity of hES to differentiate into mature hematopoietic cells in vitro. Nevertheless, there exist some quantitative and qualitative differences about hematopoietic differentiation between the hES cell lines used. However, we still have to evaluate their capacity to reconstitute hematopoiesis in vivo in an immune deficient mouse model. We will also be interested in developing in vitro methods to expand these hematopoietic precursor cells derived from hES which may be used as a viable source for future cell therapy.
Tyrosine kinase activation by Newcastle disease virus is required for TNF- alpha gene induction in astrocytes. (1994)

Astrocytes, when appropriately stimulated, produce a variety of cytokines including TNF-alpha. Production of TNF-alpha by astrocytes stimulated with Newcastle disease virus (NDV) is achieved by transcriptional activation and mRNA stabilization. A PKC-dependent pathway is responsible for a 10-fold increase in TNF-alpha mRNA stability by reducing poly(A) tail removal. The present study examined signal pathways induced by NDV in primary rat astrocytes that are responsible for TNF-alpha gene transcription as well as the possible source of kinase activity required for mRNA stabilization. Transcription of TNF-alpha gene in astrocytes stimulated by NDV or LPS and IFN-gamma was inhibited completely by the tyrosine kinase inhibitor herbimycin, and partially by a PKC inhibitor H7, as determined by nuclear run-on assay. HA-1004, a cyclic nucleotide-dependent kinase inhibitor, showed no effect. These results indicated that tyrosine kinase signaling pathways seemed to precede the activation of PKC in induction of TNF-alpha gene. Increase in tyrosine kinase activity in NDV-infected astrocytes was demonstrated by a two- to threefold increase in tyrosine phosphorylation of Pl-PLC gamma 1. Because astrocytes contain minimal Pl-PLC beta, and NDV-induced TNF-alpha mRNA was affected by pertussis toxin only modestly, Pl-PLC gamma 1 is likely the enzyme responsible for DAG generation and the PKC-dependent mRNA stabilization in response to NDV.
Dual-time-scale simulation of dynamics and reconfiguration of fiber optical networks (2012)

We describe an approach to simulate the power transients in complex (branched, ring, or mesh) transparent fiber optical networks. Simulations are performed at two time scales: first, the signal power dynamics in Erbium-doped fiber amplifiers is modeled at (sub-) millisecond scale using statistical signal descriptions. Further, the teletraffic performance of the network, such as the bit error rate, can be investigated for selected time instances at nano-second to sub-pico-second time scale using a sampled signal model (`bit-level' simulation). For the detailed simulation on `bit-level', signal information and internal states of the network components are re-used form the first simulation step at (sub-) millisecond scale. To illustrate our approach, we discuss simulations of `higher-order' transients in a ring network.
Solventborne Polymer Coatings: Drying, Film Formation, Stress Evolution, and Failure (2022)

Polymer coatings find use in a wide range of industrial applications, from conventional paints and coatings in building and construction to the pharmaceutical industry, organic solar cell production, and lithium battery technology. Despite their importance, there are gaps in our understanding of the drying process, the stress development during drying, and their influence on the final mechanical properties of the dried film. This perspective focuses on the fundamental aspects of the drying and film formation process, highlights the gaps, and suggests directions for future work.
Micellar Aqueous Phase Polymerization of Acrylamide (2011)

The kinetics of aqueous phase radical polymerization of acrylamide initiated by ammonium peroxodisulfate (APS) and 2,2 -azobisizobutyronitrile (AIBN) were studied in the presence of the non-ionic hydrophilic Tween-20 and hydrophobic Tween-85. The polymerization rate vs. conversion curve is described by a curve with two or three rate intervals and the maximal rate is located at medium conversion. This behavior strongly deviates from the kinetic model of the ideal solution polymerization with the steady-state kinetics at low and medium conversions. On the contrary, the present data indicate the kinetics typical for the transparent heterogeneous–micellar or microemulsion polymerization. The associates of polyacrylamide and acrylamide play the role of active particles. Indeed, the light scattering measurements confirmed the presence of polyacrylamide nanoparticles in both polymerization systems with and without emulsifier. The intra- and intermolecular association of polyacrylamide with its monomer is suggested to be the dominant process which induces the formation of particles responsible for the kinetics typical for the micellar or microemulsion polymerization. The experimental data show that the dependence of the maximal rate of polymerization (R p,max) vs. conversion is the result of polymerization within the nanoscaled polymer aggregates and not by the gel effect. R p,max decreases with increasing emulsifier concentration and the decrease is much more pronounced with hydrophilic emulsifier. Polymerization is faster for the APS-initiated system than for the AIBN-initiated one. The radical polymerization of AAm polymerization with and without emulsifier was discussed in terms of the formation of polymer chain aggregation (nanoparticle) into polymer chains as a main reaction locus.
A Teaching Strategy for Advanced Manufacturing Courses (2013)

In this paper, the author presents an experiment for teaching advanced manufacturing courses with the objective to maximize the learning experience based on course outcomes. A CAM course was selected to run the experiment on the students in two male sections and two female sections. The course’s outcomes were drafted based on the mechanical engineering program’s objectives. Their focus is on fulfilling as many of the program outcomes as possible. The level of fulfilling a program objective by a course outcome is then also monitored. The strategy focuses on increasing the hands on experience of the students as well as introducing more computer and technology content in the course. Every activity will be evaluated quantitatively and qualitatively. Based on the progress in a regular semester, the students’ performance is monitored and a calibration of theory versus practical experience is done accordingly. Initial results reveal that the sooner the practical part of the course is introduced the better the results will be for both understanding the practical as well as the theoretical part of the course.
Characterization of the Micro Porous Layer - Catalyst Layer Interface Structure in Polymer Electrolyte Fuel Cells (2009)

The impact of interfacial contact between the catalyst layer (CL) and diffusion medium (DM) has important implications for the transport of heat, current, and multi-phase flow in the membrane electrode assembly. This work is motivated by the need to obtain a greater understanding of the impact of the micro porous layer (MPL) and CL morphologies and resulting interfacial contact on the fuel cell performance. In this effort, an array of different virgin mating CL and MPL surface structures was investigated. The mating surface morphology of the tested MPL and CL samples were digitally characterized by optical profilometry, a non-destructive imaging technique which measures the 3-D surface topology of samples with a resolution on the order of several nanometers in the vertical direction, and 0.5 to 5 microns in the lateral direction. The benchmark data were utilized in an analytical model developed for virtual reconstruction of a compressed interfacial structure. The results show that for the materials tested, the MPL surface exhibits a higher degree of roughness compared to catalyst surfaces. The MPL surface was observed to have relatively higher roughness than the CL, indicating the potential dominance of the MPL surface morphology on the local transport and interfacial contact across the MPL|CL interface. Elasticity, compression, and uncompressed surface morphology are the key factors controlling the ultimate interfacial contact.
A large deletion in GPR98 causes type IIC Usher syndrome in male and female members of an Iranian family (2009)

A large GPR98 deletion of 136 017 bp segregates with USH2C in an Iranian family. To our knowledge, this is only the second report of a GPR98 mutation, and the first report on male subjects with USH2C and a GPR98 mutation.
Determining wrist reference kinematics using a sensory-mounted stress ball (2012)

One of the research voids in the study of home-based rehabilitation is the lack of benchmarks of the performance for various body kinematics. The objective of this work is to form a metric to evaluate the wrist motion for rehabilitation applications. The wrist motion components that were considered in this study are the angular velocity and acceleration in each plane of movement, namely Pronation/Supination, Flexion/Extension, and Radial/Ulnar Deviations. Two games were developed to measure wrist motion variables, namely the Cup and plate game (to measure the Supination/Pronation motions), and the Golf game (a horizontal version to measure the Radial/Ulnar motions and a vertical version to measure the Extension/Flexion motions). The derived values can serve as a motion benchmark to detect proper movement and steadiness of the wrist, in order to quantify the quality of patient performance.
Hyperion Next-Generation Battlespace Information Services (2007)

The future digital battlespace will be a fast-paced and frenetic environment that stresses information communication technology systems to the limit. The challenges are most acute in the tactical and operational domains where bandwidth is severely limited, security of information is paramount, the network is under physical and cyber attack and administrative support is minimal. Hyperion is a cluster of research projects designed to provide an automated and adaptive information management capability embedded in defence networks. The overall system architecture is designed to improve the situational awareness of field commanders by providing the ability to fuse and compose information services in real time. The key technologies adopted to enable this include: autonomous software agents, self-organizing middleware, a smart data filtering system and a 3-D battlespace simulation environment. This paper reviews some of the specific techniques under development within the Hyperion sub-projects and the results achieved to date.
Identification of deterministic time-lag systems (1976)

The methods of system identification due to Shinbrot, Perdreauville and Goodson, Fairman and Shen, and Diamessis belong to a class of techniques based on the idea that a linear operation on system equations leads to a set of simultaneous equations solvable for the unknown parameters. These methods have so far been able to treat problems of linear time invariant, time-varying, nonlinear, and distributed parameter systems without lags. This short paper extends the capability of the class of methods to cover time-lag systems. The extension is illustrated with reference to one of the methods that employs Poisson moment functionals. Illustrative examples include linear time invariant, time-varying, and non-linear systems each with a lag.
DEDS with state dependent event durations lookahead policies and scheduling (2005)

The authors study a discrete-event distributed system (DEDS) (a flow line) which cannot be linearized and which covers a wide range of real-world situations, from plastic molding to steel milling and the service industries, where the duration of an event (a task performed on a job) is a monotonic function of the time the job spent in the system. The present model is set in a deterministic environment, with the initial conditions (i.e., release times) as control parameters. Restricting themselves to the two-machine case for simplicity, the authors prove a closed-form formula for the optimal release times of the jobs for the minimum cycle times of a (closed-loop) repeated sequence of jobs, and for the minimum flow time of a finite sequence of jobs. The corresponding scheduling problems, together with online control policies, are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Virtual teams: considerations, advantages and disadvantages (2017)

Globalization and evolving development in information technology have reshaped how organizations conduct their daily day operations and businesses. Significant amount of human interactions are nowadays handled via different virtual communication channels. Entire projects and work tasks are increasingly done within virtual teams, and solely through different virtual channels with little physical presence. Hence, objective of this research is to investigate what are the considerations, advantages and disadvantages linked to virtual teams.&#13;\nResearch followed the qualitative research methods. Data was collected through interviews and findings were reflected to existing literature. Interviewees were all working in virtual teams which operated in financial industry and were situated in Nordic countries.&#13;\nFindings show that main advantages of virtual teams are flexibility, access to larger talent pools and networks, gaining local expertise, reduction of travel time and costs. Disadvantages show risk of misinterpretations, challenging to build trust and team feeling, lack of transparency and information sharing. Considerations related to communication methods and tools, best practices such as regular team meetings, people and communication skills, but also trust was considered to be more critical in virtual team than in traditional team.
Itinerant versus localized plasmons in an assembly of metal-dielectric parallel flat slabs in the presence of a perpendicular magnetic field: Faraday and magneto-optical Kerr effects (2021)

The influence of the surface running and surface localized plasmons on the optical properties (including Faraday and magneto-optical Kerr effects) is studied and compared for homogeneous as well as perforated metal-dielectric, sandwichlike structures. Closed-form exact expressions are derived for the macroscopic permittivity tensor of an assembly of metal-dielectric parallel flat slabs in the presence of an externally applied static magnetic field perpendicular to the slab plane. The structure-dependent resonance associated with running surface plasmon appears only in the perpendicular to the surface $zz$ macroscopic permittivity tensor component and does not depend on the perpendicularly applied magnetic field. The other diagonal tensor components vanish at the so-called ``hybrid cyclotron-plasma frequency,'' which depends on the slab thickness and the applied magnetic field. All analytical results are verified by numerical simulations.