gebhart/patrepeval huggingface dataset:
Abstract 4377: Novel RNAi agent can control HCV replication (2014)

Abstract Technique of RNA interference (RNAi) is important for investigating functional gene research and drug target. miRNA-122 (miR-122) is expressed abundantly in liver tissue, and it can control the Hepatitis C virus (HCV) replication. Locked Nucleic Acid (LNA)-miR-122 was shown to suppress HCV replication through inhibiting miR-122 function. Recently, anti-HCV therapy for chronic hepatitis C by LNA-miR-122 has been applied for clinical study in phase II. We developed alternative chemically modified sponge oligonucleotides (SO) for inhibition of interest miRNA function. In order to find more effective anti-viral agent, we attempted to compare the inhibition effects for viral replication between LNA-miR-122 and SO for miR-122. SO contained triplicated binding sites to miR-122, composed of either DNA or RNA, which were combined with terephthalate or glycine. We chose three types of sequences complementary to target miR-122: one perfect match sequence and two imperfect match sequences (bubble, and bulge). The effects of SOs on HCV replication were assessed by using HCV replicon cells (OR6), which contained full-genome HCV with genotype 1b and luciferase reporter genes. We compared the luciferase activity, the expression of HCV RNA, and the expression of HCV core protein in OR6 cells treated with LNA-miR-122 and several SO constructions. SO composed of terephthalate-bound RNA sequences with bulge-type imperfect complementarity was most effective in inhibiting HCV replication. By real-time qPCR analysis, the expression level of ISG15 and RIG-I in the OR6 cell treated with SO was not significantly higher than those treated with negative control (NC) or LNA. By XTT incorporation assay, the cell proliferation level in OR6 treated with SO was not also significantly affected as compared that treated with NC or LNA. We investigated novel RNAi agent termed SO that could effectively suppress HCV replication as compared LNA-miR-122. SO treatment did not activate the endogenous IFN pathway nor induce the cell mortality. Therefore SO is promising as a novel anti-vial agent. Citation Format: Masahiko Kuroda, Masakatsu Takanashi, Shinichiro Ohno, Yoshiki Murakami. Novel RNAi agent can control HCV replication. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4377. doi:10.1158/1538-7445.AM2014-4377
Pediatric vegetarian diets are healthful, nutritionally adequate and may provide health benefits in the prevention of obesity (2017)

Well-planned vegetarian diets are appropriate for individuals during all stages of the lifecycle, including pregnancy, lactation,infancy, childhood, and adolescence. Vegetarian Diets in Perspective: According to a nationwide pollen 2016, approximately 3.3% of American adults are vegetarian or vegan and about 46% of vegetarians are vegan. Plant- based diets are becoming well accepted. The American Institute for Cancer Research encourages a plant-based diet. The 2015-2020 dietary Guidelines for Americans recommend a veteran approach for the National School Lunch Program. A vegetarian is a person who consumes all plant foods, does not eat animal foods, including fowl or seafood, or products containing animal foods. The eating patterns of vegetarians may vary considerably. There are basically three types of vegetarian diets. 1. The lacto-ovo-vegetarian eating pattern, the most common type is based on grains, vegetables, fruits, legumes, nuts, seeds, dairy products, and eggs. 2. Lactovegetarian Diet includes milk with plant foods but excludes any other foods from animals such as eggs. 3. Total Vegetarian or Plant based Diet is made of grains, fruits, vegetables, legumes, nuts, seeds, excludes the use of all animal products. Vegan means no animal products excluding the wearing of leather products. There are many reasons for the rising interest in vegetarian diets. Health, economic, ecological, ethical or religious reasons are at the top five. Scientific research continues to document the health advantages of the vegetarian diet with lower risk of heart related diseases, obesity, and cancer. Many are starting their children on a vegetarian lifestyle for the major reason to maintain good health and to prevent diet related diseases. The number of vegetarians in the United States and Canada is expected to increase during the next decade. Food and nutrition professionals can assist vegetarian clients by providing current, accurate information to parents about vegetarian nutrition, diet and resources.
Valutare la qualità dell'offerta formativa territoriale : un quadro di riferimento (2010)

Isfol, Di Battista G. e Palomba L. e Vergani A. (a cura di), Valutare la qualita dell'offerta formativa territoriale : un quadro di riferimento, Roma, Isfol, 2010. Isfol OA
TRAC in high-content gene expression analysis: applications in microbial population studies, process biotechnology and biomedical research (2008)

More than a decade of intensive use of microarray technology has flooded the scientific community with genome-wide expression data of diverse biological states. As a result, connection of the expression signatures of a relatively small number of genes related to, for example, disease states, patient responses or toxicological responses has become possible. Development of tools that enable cost- and time-efficient analysis of such signatures from large sample numbers is currently of major interest for research, drug screening and diagnostic purposes. A method named transcript analysis with aid of affinity capture (TRAC) is a novel solution hybridization and bead-based assay enabling multiplex mRNA target detection simultaneously from large sample numbers. Functionality of TRAC has been shown in a number of applications, including microbial quantification, gene expression-based monitoring of biotechnical processes, cell-based cancer marker gene screening and siRNA validation, which are reviewed here.
Optimization of Labyrinth Seal for Screw Compressor (2007)

There is a growing demand for compressed air in the industry for various applications. Majority of industrial requirements is in line with screw compressor operating range. Design and construction of screw compressors are demanding tasks that require advanced calculations and theoretical knowledge. Clearances play a major role in the performance and reliability aspects of a screw compressor. Seals are provided in compressors to fit around rotor shafts in order to prevent the leakage of lubricating oil and working medium. However there is a small clearance between the seal and rotor shaft, which can cause potential leakage of the working medium. The performance of the compressor is directly related to the leakage rate through the seals. The labyrinth seal is a special type of seal, used in screw compressors and turbo-machinery for sealing purpose. Labyrinth seal is a non-contacting type seal that uses a tortuous path to minimize the gas leakage. The pressure drop occurs at each labyrinth tooth as the medium is squeezed between the labyrinth tooth and the rotor. The leakage through the seal is directly related to the labyrinth profile and also the clearance between the rotor and the labyrinth tooth. The present work is carried out to reduce the leakage through the labyrinth seal by optimising the tooth profile and operating clearances. Heat transfer analysis is carried out on the housing of the labyrinth seal to find out the boundary temperature of the seal. Also the heat transfer analysis on the labyrinth seal followed by Thermo-structural analysis is carried out to find out the accurate operating clearance of the seal. By using CFD as a tool, the optimisation is carried out on different design configurations of labyrinth seal by comparing the deviation in leakage rates. Effect of rotor speed, width of seal and pressure ratio on air leakage rate is also investigated. A set of labyrinth seals has been designed based on the above optimisation and tested in the compressor. The results have been compared with the CFD prediction.
Robust artificial noise-aided beamforming for a secure MISO-NOMA visible light communication system (2020)

Visible light communication (VLC) and non-orthogonal multiple access (NOMA) have been deemed two promising techniques in the next wireless communication networks. In this paper, secure communications in the presence of potential eavesdropper are investigated for a multiple-input single-output VLC system with NOMA. The artificial noise jamming and beamforming technologies are applied to improve secure performance. A robust resource allocation scheme is proposed to minimize the total transmit power taking into account the constraints on the quality of service requirement of the desired users and the maximum tolerable data rate of the eavesdropper, and the practical imperfect channel state information of both the desired users and the eavesdropper. The formulated non-convex optimization problem is tackled based on S-Procedure and semi-definite programming relaxation. Simulation results illustrate that our proposed resource allocation scheme can effectively guarantee communication security and achieve transmit power saving. Moreover, the height and number of LED can significantly affect system performance and the optimum LED height can be obtained for different LED numbers.
Monolithic colliding-pulse mode-locked quantum-well lasers (1992)

Integration of the whole mode-locked laser onto a single piece of semiconductor offers a number of advantages, including total elimination of optical alignment processes, improved mechanical stability, and the generation of short optical pulses at much higher repetition frequencies. Semiconductor laser processing technologies were used to implement the colliding-pulse mode-locking (CPM) scheme, which is known to effectively shorten the pulses and increase stability, on a miniature monolithic semiconductor cavity. The principles of and recent progress in monolithic CPM quantum-well lasers are reviewed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Comparison of the Accessory Activity of Murine Peritoneal Cavity Macrophage Derived Dendritic Cells and Peritoneal Cavity Macrophages in a Mixed Lymphocyte Reaction. (2001)

A detailed comparison of the accessory cell activities was carried out among murine peritoneal cavity macrophages (PEC-Mphi), peritonea] cavity macrophages stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4), the most popular cytokine combination widely used to generate dendritic cells (DC) and peritoneal cavity macrophage-derived DC (PEC-DC) using a two-way mixed lymphocyte reaction (MLR). All the cell types used efficiently induced statistically significant naïve T cell proliferation at all culture time points and responder:stimulator ratios used. However, marked differences were noted in the magnitude of the proliferative responses. These variations may be attributed to the intensity of expression of MHC class II glycoproteins, as well as the actual numbers of MHC class II+ cells.
Rubidium as an Alternative Cation for Efficient Perovskite Light-Emitting Diodes (2018)

Incorporation of rubidium (Rb) into mixed lead halide perovskites has recently achieved record power conversion efficiency and excellent stability in perovskite solar cells. Inspired by these tremendous advances in photovoltaics, this study demonstrates the impact of Rb incorporation into MAPbBr<sub>3</sub>-based light emitters. Rb partially substitutes MA (methyl ammonium), resulting in a mixed cation perovskite with the formula MA<sub>(1- x)</sub>Rb <sub>x</sub>PbBr<sub>3</sub>. Pure MAPbBr<sub>3</sub> crystallizes into a polycrystalline layer with highly defective sub-micrometer grains. However, the addition of a small amount of Rb forms MA<sub>(1- x)</sub>Rb <sub>x</sub>PbBr<sub>3</sub> nanocrystals (10 nm) embedded in an amorphous matrix of MA/Rb Br. These nanocrystals grow into defect-free sub-micrometer-sized crystallites with further addition of Rb, resulting in a 3-fold increase in exciton lifetime when the molar ratio of MABr/RbBr is 1:1. A thin film fabricated with a 1:1 molar ratio of MABr/RbBr showed the best electroluminescent properties with a current efficiency (CE) of 9.45 cd/A and a luminance of 7694 cd/m<sup>2</sup>. These values of CE and luminance are, respectively, 19 and 10 times larger than those achieved by pure MAPbBr<sub>3</sub> devices (0.5 cd/A and 790 cd/m<sup>2</sup>). We believe this work provides important information on the future compositional optimization of Rb<sup>+</sup>-based mixed cation perovskites for obtaining high-performance light-emitting diodes.
Reply from the Author (1995)

Reply from the Authors: We appreciate Dr. Pascual's comments regarding indomethacin-resistant HC. We too have observed patients with …
Implementation of a prototype active network (2002)

Active networks carry SmartPackets that contain both data and user-specified methods to extend the functionality of switches to perform user-specific computation on the encapsulated data. This enables rapid customization of the network and provides a mechanism to adapt the network to a changing environment. We describe the implementation of a prototype active network that is implemented in Java. We also illustrate the use of active networks to provide enhanced services at both the network and application levels.
Staphylococci as urinary pathogens. (1977)

During the course of one year all (382) strains of staphylococci isolated in significant culture from urine specimens were typed by the Baird-Parker method. Staphylococcus aureus accounted for only 63 (16%) of the infections. Novobiocin-resistant micrococcal infection occurred predominantly in young women but also in children of both sexes; it was not restricted to M3. To try to detect possible sources of micrococcal infection other than faeces the normal flora of the throat, urinary tract, and vagina of young women was studied. Novobiocin-resistant micrococci were rarely found. Previous reports that micrococci are the second commonest urinary pathogens in young women in domiciliary practice were confirmed. The laboratory records of patients with these infections suggested that they respond well to treatment and that recurrences are usually due to a different organism.
Treatment of major venous obstruction with an expandable endoluminal spiral prosthesis. (1989)

Six patients with extensive iliofemoral and/or caval thrombosis were treated by thrombectomy and subsequent implantation of an expandable endoluminal spiral prosthesis in case of congenital caval stenoses (1) or extravascular compression or traction (5). Five of these patients had undergone previous surgery and thrombosis had developed despite low dose heparin given postoperatively. Three patients had had an unsuccessful thrombectomy prior to spiral implantation. No complications related to the endoluminal prosthesis occurred. There was one retroperitoneal hematoma from guide wire perforation of the inferior vena cava (IVC) necessitating laparotomy and reversal of the previously constructed femoral av-fistula with subsequent iliofemoral rethrombosis. All other endoluminally reconstructed veins remained open at early and late (up to 12 months) review confirmed by phlebography. We conclude that with increasing clinical experience endoluminal relining of obstructed major veins will probably become a valuable method of venous reconstruction with minimal surgical trauma.
Treatment of Pesticide-Contaminated Water Using a Selected Fungal Consortium: Study in a Batch and Packed-Bed Bioreactor (2021)

This study provides the basis for implementing a continuous treatment system for wastewater containing a pesticide mixture formed by atrazine, iprodione, and chlorpyrifos. Two fungal strains (Verticilium sp. H5 and Metacordyceps sp. H12) isolated from a biomixture of a biopurification system were able to remove different pesticide concentrations (10 to 50 mg L−1) efficiently from the liquid medium; however, the half-life of the pesticides was reduced and characterized by a T1/2 of 5.4 to 9.2 d for atrazine, 3.7 to 5.8 d for iprodione, and 2.6 to 2.9 d for chlorpyrifos using the fungal consortium. The immobilization of the fungal consortium in alginate bead was effective, with the highest pesticide removal observed using an inoculum concentration of 30% wv−1. The packed-bed reactor with the immobilized fungal consortium, which was operated in the continuous mode at different flow rates (30, 60, and 90 mL h−1), required approximately 10 d to achieve removal efficiency (atrazine: 59%; iprodione: 96%; chlorpyrifos: ~85%). The bioreactor was sensitive to flow rate fluctuations but was able to recover performance quickly. The pesticide metabolites hydroxyatrazine, 3,5-dichloroaniline, and 3,5,6-trichloro-2-pyridinol were produced, and a slight accumulation of 3,5,6-trichloro-2-pyridinol was observed. Nevertheless, reactor removal efficiency was maintained until the study ended (60 d).
CMUT fabrication based on a thick buried oxide layer (2010)

We introduce a versatile fabrication process for direct wafer-bonded CMUTs. The objective is a flexible fabrication platform for single element transducers, 1D and 2D arrays, and reconfigurable arrays. The main process features are: A low number of litho masks (five for a fully populated 2D array); a simple fabrication sequence on standard MEMS tools without complicated wafer handling (carrier wafers); an improved device reliability; a wide design space in terms of operation frequency and geometric parameters (cell diameter, gap height, effective insulation layer thickness); and a continuous front face of the transducer (CMUT plate) that is connected to ground (shielding for good SNR and human safety in medical applications). All of this is achieved by connecting the hot electrodes individually through a thick buried oxide layer, i.e. from the handle layer of an SOI substrate to silicon electrodes located in each CMUT cell built in the device layer. Vertical insulation trenches are used to isolate these silicon electrodes from the rest of the substrate. Thus, the high electric field is only present where required - in the evacuated gap region of the device and not in the insulation layer of the post region. Array elements (1D and 2D) are simply defined be etching insulation trenches into the handle wafer of the SOI substrate.
Vagus nerve stimulation for complex partial seizures: surgical technique, safety, and efficacy (1993)

Electrical stimulation of the vagus nerve has shown efficacy in controlling seizures in experimental models, and early clinical trials have suggested possible benefit in humans. Eleven patients with complex partial seizures were subjected to implantation of vagus nerve stimulators. Electrode contacts embedded in silicone rubber spirals were placed on the left vagus nerve in the low cervical area. A transcutaneously programmable stimulator module was placed in an infraclavicular subcutaneous pocket and connected to the electrode. One patient required replacement of the system due to electrode fracture. Another patient developed delayed ipsilateral vocal-cord paralysis; the technique was then modified to allow more tolerance for postoperative nerve edema. A third patient showed asymptomatic vocal-cord paresis on immediate postoperative laryngoscopy. Vagus nerve stimulation produces transient vocal-cord dysfunction while the current is on. Nine patients were randomly assigned to receive either high- or low-current stimulation, and seizure frequency was recorded. The high-current stimulation group showed a median reduction in seizure frequency of 27.7% compared to the preimplantation baseline, while the low-current stimulation group showed a median increase of 6.3%. This difference approached statistical significance. The entire population then received maximally tolerable stimulation. The high-current stimulation group showed a further 14.3% reduction, while the low-current stimulation group showed a 25.4% reduction compared to the blinded period. The efficacy of vagus nerve stimulation seemed to depend on stimulus parameters, and a cumulative effect was evident. These results are encouraging, and further study of this modality as an adjunct treatment for epilepsy is warranted.
Human eotaxin induces alpha 4 and beta 2 integrin-dependent eosinophil accumulation in rat skin in vivo: delayed generation of eotaxin in response to IL-4. (1998)

The CC chemokine eotaxin, originally purified from bronchoalveolar lavage fluid of sensitized guinea pigs following allergen challenge, is a potent eosinophil-selective chemoattractant. In the present study, we have used (111)In-eosinophils and human eotaxin to characterize the profile of chemokine-induced eosinophil accumulation in vivo in rat skin. Intradermally injected eotaxin caused a dose-dependent accumulation of (111)In-eosinophils. Time course studies indicated that the response was rapid, since all the accumulation occurred within the first 1 to 2 h of eotaxin injection. The i.v. administration of anti-intercellular adhesion molecule-1, anti-vascular cell adhesion molecule-1, or anti-alpha4 integrin mAbs significantly inhibited the eosinophil accumulation induced by 100 pmol of human eotaxin by 73, 43, and 67%, respectively. Further, when (111)In-eosinophils were pretreated in vitro with anti-alpha4 integrin or anti-beta2 integrin mAbs, or with a combination of both mAbs, eotaxin-induced responses in vivo were reduced by 52, 49, and 68%, respectively. Eosinophil accumulation induced by intradermal IL-4, but not that induced by TNF-alpha or leukotriene B4, appeared to be mediated in part by endogenously generated eotaxin. Anti-eotaxin Abs significantly inhibited (54%) the later phases (24-28 h) but not the early phase (0-4 h) of the response to IL-4. This was consistent with eotaxin mRNA expression peaking at 18 h after IL-4 injection. Our findings show that human eotaxin is a potent inducer of eosinophil accumulation in vivo, this response being dependent on alpha4 integrin/vascular cell adhesion molecule-1 and beta2 integrin/intercellular adhesion molecule-1 adhesion pathways. Further, the eosinophil accumulation in response to IL-4 is partly mediated by endogenously generated eotaxin.
A new methodological approach to evaluation of mechanical resistance of green plantings in urban environments (2018)

The trees growing in urban environments are exposed to the highest pressure of negative exogenous factors and, consequently, many tree species come to the critical age and become hazardous. A complex approach to evaluate the condition of green plantings under the technogenous load using visual, instrumental, biomechanical etc. methods allows timely and reliable identification of hazardous trees. For this purpose, we developed a new methodology of determining the hazardous degree of trees in residential areas. This method is based on the accounts of tree mechanical hardiness evaluation and up-to-date instrumental research data to be used by municipal services employed in plantings monitoring and care. The main parameters are: age, diameter of the base, diameter at height, trunk girth at the base, trunk girth at a height of 1,3 m, the height of the tree, the angle of inclination of the trunk, the vital status score for Saveleva, morphological damage to the trunk, the description of the crown architectonics, the presence of morphogenetic and exogenous damage to the leaf blade, the population of the trunk pests, the presence of hollows, the wind feature and the loading of this site, the mode of visiting the territory (recreational load), the direction of possible fall, percentage of sound rot, modulus of elasticity of wood, density of wood, the ratio of biomass to critical mass, mechanical resistance to static and dynamic loads according to calculations on biomechanics. In the case study of a model tree (London plane) the applicability of this approach to landscaping practices is shown.