gebhart/patrepeval huggingface dataset:
Manufacturing ultra-concentrated liquid feeds: Transitioning the aqueous solubility barrier of the feed amino acids cysteine and tyrosine (2015)

Background Fed-batch cultivation is currently the most prominent process for the production of Biologics using mammalian cell lines such as CHO cells. Performance of fedbatch processes greatly depends on the feeding strategy. One important aspect of feeds is the nutrient concentration, which should be as high as possible. However, for some nutrients, there are solubility and/or stability limitations in aqueous solution. Thus, alternative systems for liquid feed manufacturing were considered. Deep Eutectic Solvents (DES) are salts in liquid state with a melting point under 100°C in the absence of water that form a eutectic system with a lower melting point than either of the individual components. The result is a highly concentrated, water free liquid system. A common component of DES is choline chloride (ChCl), a quaternary ammonium salt. For cell culture applications, ChCl is particularly well suited, since it is already widely used as cell culture medium component. We have experimentally manufactured deep eutectic solvents (DES) for application as cell culture feeds containing tyrosine and cysteine, which are challenging due to their weak solubility and/or stability in aqueous solution.
Effect of Size and Concentration of Latex Particles on Respiration of Human Blood Leucocytes. (1961)

Rigorously standardized polystyrene latex particles of graded sizes were added to whole blood, and oxygen uptake by leucocytes as they ingested these particles was measured by the direct method of Warburg. The importance of both size and concentration of particles was established.
Extended enantiopure ortho-phenylene ethylene (o-OPE)-based helical systems as scaffolds for supramolecular architectures: a study of chiroptical response and its connection to the CISS effect (2021)

Versatile enantiopure helical systems are described and are of interest owing to their intense chiroptical responses, their attractive architecture for metallosupramolecular chemistry and CISS effect.
The Conceptual Design of a Mechatronic System to Handle Bedridden Elderly Individuals (2016)

The ever-growing percentage of elderly people in developed countries have made Ambient Assisted Living (AAL) solutions an important subject to be explored and developed. The increase in geriatric care requests are overburdening specialized institutions that cannot cope with the demand for support. Patients are forced to have to remain at their homes encumbering the spouse or close family members with the caregiver role. This caregiver is not always physically and technically apt to assist the bedridden person with his/her meals and hygiene/bath routine. Consequently, a solution to assist caregivers in these tasks is of the utmost importance. This paper presents an approach for supporting caregivers when moving and repositioning Bedridden Elderly Peoples (BEPs) in home settings by means of a mechatronic system inspired by industrial conveyers. The proposed solution is able to insert itself underneath the patient, due to its low-profile structural properties, and retrieve and reallocate him/her. Ideally, the proposed mechatronic system aims to promote autonomy by reducing handling complexity, alter the role of the caregiver from physically handler of the BEP to an operator/supervisor role, and lessen the amount of effort expended by caregivers and BEPs alike.
Platelets and Stromal Cell-Derived Factor-1 in Progenitor Cell Recruitment (2007)

Stromal cell-derived factor-1 (SDF-1) is a CXC chemokine that binds to its sole counterreceptor, CXCR4. It is well reported that the SDF-1/CXCR4 signaling pathway is of vital importance to human development and to various pathophysiological phenomena, including hematopoiesis, angiogenesis, atherosclerosis, cancer growth, metastasis, and human immunodeficiency virus infection. SDF-1 promotes mobilization of bone marrow-derived endothelial progenitor cells (EPCs) to the circulation in response to vascular injury. Recently, we found that platelets express and release SDF-1 into the microcirculation upon activation and we observed that platelet-derived SDF-1 is functionally involved in recruitment of EPCs to arterial thrombi in vivo. This review discusses the unique functions of this chemokine and the newly discovered impact of platelet-derived SDF-1 into the recruitment of progenitor cells to vascular injury areas, and its subsequent effects in atherosclerosis, vascular repair, and angiogenesis.
Immobilization-induced changes in erythrocyte membrane fluidity in rabbits: a spin-label electron spin resonance study. (2002)

It has been shown that many diseases are linked to abnormalities of the erythrocyte membrane. This study observed the changes in erythrocyte membrane fluidity during an immobilization period of 21 days. The right hindlimbs of male adult New Zealand white rabbits were immobilized for 21 days. Blood samples were collected and heparinized prior to immobilization and on days 1, 3, 7, 14, and 21 of immobilization. The membrane fluidity of erythrocytes was measured by spin-label electron spin resonance (ESR). The membrane fluidity was expressed by the value of order parameter (S). The results showed a significant increase of S on days 7 and 14 of immobilization (p <0.01). The highest value of S was found on day 7 of immobilization; thereafter, S gradually declined. Compared with the value measured before immobilization, there was no significant difference in the value of S on day 21. The results show that immobilization significantly affected erythrocyte membrane fluidity, indicating a systemic and temporal response ofthe immobilized body. This study is the first to document the time-course of changes in erythrocyte membrane fluidity in rabbits with single hindlimb immobilization during 21 days. These results may be helpful in studying the pathophysiology of immobilization.
Influence of erbium doping on the femtosecond laser damage characteristics of fluorozirconate glasses (2023)

In this study, we systematically investigated femtosecond laser-induced damage characteristics of ZHBLAN (27ZrF 4 –25HfF 4 –10BaF 2 –5SrF 2 –5CaF 2 –4LaF 3 –14AlF 3 –10NaF), and the impact of Er 3+ doping on the optical characteristics and damage characteristics of the matrix material. The glasses were subjected to a comparative analysis with respect to typical ZBLAN glasses. The sizes and morphology of damaged craters changed with the power of the 800 nm femtosecond laser. The samples were analyzed using digital and scanning electron microscopes, and their femtosecond laser-induced damage thresholds (LIDTs) were calculated by linear regression. The LIDTs decreased obviously from 612 mJ/cm 2 to 532 mJ/cm 2 after Er 3+ was doped into the ZHBLAN glass. This is related to the absorption of Er 3+ near the wavelength of the femtosecond laser and the change in the bandgap structure of the glass due to Er 3+ doping. In addition, we have analyzed the electron convergence rate within the material during laser irradiation. The combination of physical models and simulation results reveals the association between the ionization rate of optical materials and the laser damage threshold.
Potential of selective sodium-hydrogen exchange inhibitors in cardiovascular therapy (1995)

Journal Article Potential of selective sodium-hydrogen exchange inhibitors in cardiovascular therapy Get access Wolfgang Scholz, Wolfgang Scholz * Hoechst AG, SBU Cardiovascular Agents, H821, 65926 Frankfurt/Main, Germany *Correspondence to Dr Scholz. Search for other works by this author on: Oxford Academic PubMed Google Scholar Udo Albus Udo Albus Hoechst AG, SBU Cardiovascular Agents, H821, 65926 Frankfurt/Main, Germany Search for other works by this author on: Oxford Academic PubMed Google Scholar Cardiovascular Research, Volume 29, Issue 2, February 1995, Pages 184–188, https://doi.org/10.1016/S0008-6363(96)88568-3 Published: 01 February 1995 Article history Received: 28 April 1994 Accepted: 01 September 1994 Published: 01 February 1995
EphA2 receptor is a key player in the metastatic onset of Ewing sarcoma (2018)

Ewing sarcoma (ES) is the second most common bone malignancy affecting children and young adults with poor prognosis due to high metastasis incidence. Our group previously described that EphA2, a tyrosine kinase receptor, promotes angiogenesis in Ewing sarcoma (ES) cells via ligand-dependent signaling. Now we wanted to explore EphA2 ligand-independent activity, controlled upon phosphorylation at S897 (p-EphA2<sup>S897</sup> ), as it has been linked to metastasis in several malignancies. By reverse genetic engineering we explored the phenotypic changes after EphA2 removal or reintroduction. Gene expression microarray was used to identify key players in EphA2 signaling. Mice were employed to reproduce metastatic processes from orthotopically implanted engineered cells. We established a correlation between ES cells aggressiveness and p-EphA2<sup>S897</sup> . Moreover, stable overexpression of EphA2 in low EphA2 expression ES cells enhanced proliferation and migration, but not a non-phosphorylable mutant (S987A). Consistently, silencing of EphA2 reduced tumorigenicity, migration and invasion in vitro, and lung metastasis incidence in experimental and spontaneous metastasis assays in vivo. A gene expression microarray revealed the implication of EphA2 in cell signaling, cellular movement and survival. ADAM19 knockdown by siRNA technology strongly reproduced the negative effects on cell migration observed after EphA2 silencing. Altogether, our results suggest that p-EphA2<sup>S897</sup> correlates with aggressiveness in ES, so blocking its function may be a promising treatment.
Forced Medication in Psychiatry: Patients' Rights and the Law Not Respected by Appeals Board in Denmark. (2019)

Patients' rights and the law were not being respected. We suggest forced medication be abandoned, in accordance with the United Nations Convention on the Rights of Persons with Disabilities.
Comparison of eddy viscosity-transport turbulence models for three-dimensional, shock-separated flowfields (1994)

An evaluation of four one-equation eddy viscosity-transport turbulence closure models as applied to three-dimensional shock wave/boundary-layer interactions is presented herein. Comparisons of two versions of the Baldwin-Barth model, an approach of Edwards and McRae, and a modified form of the Spalart-Allmaras model are presented for two test cases, one involving Mach 8 flow over a flat plate/sharp fin apparatus and the other involving Mach 3 flow over a cylinder-offset-cone geometry. Strengths and weaknesses of the one-equation approaches are highlighted through direct comparison with experimental data, and the effect of grid refinement is examined.
Robust Testing in Outpatient Settings to Explore COVID-19 Epidemiology: Disparities in Race/Ethnicity and Age, Salt Lake County, Utah, 2020 (2021)

We found disparities by race/ethnicity and age in access to testing and in receiving a positive test result among outpatients tested for SARS-CoV-2. Further research and public health outreach on addressing racial/ethnic and age disparities will be needed to effectively combat the coronavirus disease 2019 pandemic in the United States.
Alpha Tocopherol Protects Against Immunosuppressive and Immunotoxic Effects of Lead (2003)

Chronic exposure to lead (Pb) is associated with multiorgan toxicity. The precise mechanism(s) involved, however, remains incompletely defined. The present study was undertaken to analyze the effect of Pb on the immune system and determine the ability of alpha tocopherol (AT) to reverse Pb-induced immunotoxicity. Groups of TO Mice (6 per group) were treated ip for 2 weeks with saline alone, Pb acetate alone, Pb plus AT, or with AT alone. Spleens were then analyzed for (i) cellular composition by flow cytometry, (ii) cellular response to B and T cell mitogens and (iii) production of nitric oxide (NO). Pb treatment resulted in a significant state of splenomegaly associated mainly with an influx of CD11b+ myeloid cells. Surprisingly, however, these cells exhibited no upregulation in expression of activation markers and did not produce NO. The lymphocyte mitogenic responses were inhibited by > or = 70% in Pb-treated group. Concurrent treatment with Pb and AT resulted in almost a complete reversal of Pb-induced splenic cellular influx. Despite this, however, mitogenic responses in Pb + AT treated group were approximately 50% of those observed in normal (saline-treated) controls. We conclude that (1) chronic treatment with Pb acetate induces a state of splenomegaly and decreased proliferation in response to mitogenic stimuli and (2) co-treatment with AT largely reversed the cellular influx but this was associated with only a partial improvement of the mitogenic responses. These results highlight the role of AT as a potentially effective antioxidant in the immune system.
RE: CYTOKERATIN 20 (1999)

No AccessJournal of UrologySociety of Urologic Oncology: Letters to the Editor1 Aug 1999RE: CYTOKERATIN 20A NEW MARKER FOR EARLY DETECTION OF BLADDER CELL CARCINOMA J. Southgate and P. Harnden J. SouthgateJ. Southgate and P. HarndenP. Harnden View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)68611-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "RE: CYTOKERATIN 20." The Journal of Urology, 162(2), pp. 501–502 References 1 : Expression of cytokeratin 20 in urinary cytology of patients with bladder carcinoma. Cancer1998; 82: 349. Google Scholar 2 : Normal human urothelial cells in vitro: proliferation and induction of stratification. Lab. Invest.1994; 71: 583. Google Scholar 3 : Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell. Biol.1990; 111: 567. Google Scholar 4 : Molecular diversity of cytokeratins: significance for cell and tumor differentiation. Acta Histochemica1991; 41: 117. Google Scholar 5 : Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Amer. J. Path.1992; 140: 427. Google Scholar 6 : Cytokeratin 20 expression by non-invasive transitional cell carcinomas: potential for distinguishing recurrent from non-recurrent disease. Histopathology1995; 27: 169. Google Scholar 7 : Cytokeratin 20 as an objective marker of urothelial dysplasia. Brit. J. Urol.1996; 78: 870. Google Scholar 8 : Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet1999; 353: 974. Google Scholar 9 : Cytokeratin 14 as a marker of squamous differentiation in transitional cell carcinomas. J. Clin. Path.1997; 50: 1032. Google Scholar 10 : Editorial: markers in bladder cancer-issues to consider. J. Urol.1998; 160: 2009. Link, Google Scholar 11 : Expression of cytokeratin 20 in urinary cytology of patients with bladder carcinoma. Cancer1998; 83: 1052. Google Scholar ICRF Cancer Medicine and St. James's University Hospital; Beckett Street; Leeds, LS9 7TF; United Kingdom© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byINOUE T, NAKANISHI H, INADA K, HIOKI T, TATEMATSU M and SUGIMURA Y (2018) REAL TIME REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION OF URINARY CYTOKERATIN 20 DETECTS TRANSITIONAL CELL CARCINOMA CELLSJournal of Urology, VOL. 166, NO. 6, (2134-2141), Online publication date: 1-Dec-2001. Volume 162Issue 2August 1999Page: 501-502 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.MetricsAuthor Information J. Southgate More articles by this author P. Harnden More articles by this author Expand All Advertisement PDF downloadLoading ...
Novel and Direct Access to the Human Locomotor Spinal Circuitry (2010)

The degree of automaticity of locomotion in primates compared with other mammals remains unclear. Here, we examine the possibility for activation of the spinal locomotor circuitry in noninjured humans by spinal electromagnetic stimulation (SEMS). SEMS (3 Hz and 1.3-1.82 tesla) at the T11-T12 vertebrae induced involuntary bilateral locomotor-like movements in the legs of individuals placed in a gravity-neutral position. The formation of locomotor-like activity during SEMS started with a latency of 0.68 +/- 0.1 s after delivering the first stimulus, unlike continuous vibration of muscles, which requires several seconds. The first EMG burst in response to SEMS was observed most often in a proximal flexor muscle. We speculate that SEMS directly activates the circuitry intrinsic to the spinal cord, as suggested by the immediate response and the electrophysiological observations demonstrating an absence of strictly time-linked responses within the EMG burst associated with individual stimuli during SEMS. SEMS in the presence of vibration of the leg muscles was more effective in facilitating locomotor-like activity than SEMS alone. The present results suggest that SEMS could be an effective noninvasive clinical tool to determine the potential of an individual to recover locomotion after a spinal cord injury, as well as being an effective rehabilitation tool itself.
Technology Readiness of Future Generation Networks Leveraging Regenerative Satellite Mesh Architecture ¿ a Spaceway Perspective (2006)

Satellite network capacity, adaptability, and responsiveness are enhanced with onboard capabilities for packet switching, bandwidth allocation, and spot-beams which facilitate uplink and downlink spectral reuse. A recent over-the-air (OTA) test of the SPACEWAY /sup TM/system, a Ka-band regenerative satellite mesh network supporting IP packet services, provides definitive demonstration of key capabilities in the areas of quality-of-service, routing for unicast and multicast (both best-effort and guaranteed service) traffic, dynamic bandwidth resource allocation, security, and configurable satellite uplink and downlink components. Leveraging SPACEWAY system technologies and operational capabilities serves as a pragmatic step toward the development of future multi-satellite networks with more advanced features including onboard packet routing, multi-mode radio transmission, and inter-satellite links, which are now being considered for transformational satellite networks
Truncation analysis of an alkaline cellulase from an alkalophilicBacillusspecies (1987)

A series of truncated gene products from an alkaline cellulase from an alkalophilic &lt;it&gt;Bacillus&lt;/it&gt; sp. No. 1139 has been prepared. The variously sized proteins were products of in vitro insertional mutagenesis constructs made by gene inserts containing translational terminators. One product, a 46-kDa protein, which had about half the &lt;it&gt;M&lt;/it&gt;&lt;inf&gt;r&lt;/inf&gt; of the original cellulase, had a similar enzyme activity and pH optimum to the original 92-kDa protein. In contrast, a slightly smaller product protein (43 kDa) did not show cellulase activity.
Mushroom cultivation in the circular economy (2018)

Commercial mushrooms are produced on lignocellulose such as straw, saw dust, and wood chips. As such, mushroom-forming fungi convert low-quality waste streams into high-quality food. Spent mushroom substrate (SMS) is usually considered a waste product. This review discusses the applications of SMS to promote the transition to a circular economy. SMS can be used as compost, as a substrate for other mushroom-forming fungi, as animal feed, to promote health of animals, and to produce packaging and construction materials, biofuels, and enzymes. This range of applications can make agricultural production more sustainable and efficient, especially if the CO<sub>2</sub> emission and heat from mushroom cultivation can be used to promote plant growth in greenhouses.
Time-of-Flight Laserkamera mit großer Empfangsapertur (2014)

Die vorliegende Dissertation beschäftigt sich mit der Verbesserung von 3D-Scannern, die ihre räumliche Umgebung nach dem Prinzip der Lichtlaufzeitmessung erfassen. Dabei steht die Problematik der Erreichung einer großen Empfangsapertur bei gleichzeitig schnellem Scanvorgang und ingenieurwissenschaftlich sinnvoller Systemkomplexität im Mittelpunkt der Untersuchungen. Hintergrund dieser Zielsetzung ist die Erhöhung der Empfangsleistung und damit der Reichweite aktueller Systeme.
Novel expression of Haemonchus contortus vaccine candidate aminopeptidase H11 using the free-living nematode Caenorhabditis elegans (2013)

With the problem of parasitic nematode drug resistance increasing, vaccine development offers an alternative sustainable control approach. For some parasitic nematodes, native extracts enriched for specific proteins are highly protective. However, recombinant forms of these proteins have failed to replicate this protection. This is thought to be due to differences in glycosylation and/or conformation between native and recombinant proteins. We have exploited the free-living nematode Caenorhabditis elegans to examine its suitability as an alternative system for recombinant expression of parasitic nematode vaccine candidates. We focussed on Haemonchus contortus aminopeptidase H11 glycoprotein, which is enriched in a gut membrane fraction capable of inducing significant protection against this important ovine gastrointestinal nematode. We show that H. contortus H11 expressed in C. elegans is enzymatically active and MALDI mass spectrometry identifies similar di- and tri-fucosylated structures to those on native H11, with fucose at the 3- and/or 6-positions of the proximal GlcNAc. Some glycan structural differences were observed, such as lack of LDNF. Serum antibody to native H11 binds to C. elegans recombinant H11 and most of the antibody to rH11 or native H11 is directed to glycan moieties. Despite these similarities, no reduction in worm burden or faecal egg count was observed following immunisation of sheep with C. elegans-expressed recombinant H11 protein. The findings suggest that the di- and tri-fucosylated N-glycans expressed on rH11 do not contribute to the protective effect of H11 and that additional components present in native H11-enriched extract are likely required for enhancing the antibody response necessary for protection.
Development of a 1‐Methylcyclopropene (1‐MCP) Sachet Release System (2006)

ABSTRACT The partitioning of 1‐methylcyclopropene (1‐MCP) between the gas/polymer matrix was determined for 2 adsorbing agents and 4 sachet materials to estimate the adsorption potential of 1‐MCP at 23°C. The release study was performed using a closed system under 2 different environmental conditions, dry air (0% RH) and 90% RH. Sachets made from Tyvek®, paper, low‐density polyethylene (LDPE), and polyvinyl acetate (PVA) materials were fabricated to contain silica gel and activated carbon. Activated carbon sachets did not release 1‐MCP at either testing condition. Activated carbon had a very strong affinity for 1‐MCP. The permeability coefficients of 1‐MCP and water in polyethylene and polyvinyl acetate films were determined using a quasi‐isostatic method. LDPE sachets containing silica gel had similar 1‐MCP release rates at both 0% and 90% RH. PVA sachets containing silica gel had slow release of 1‐MCP. The amount of 1‐MCP released from PVA sachets containing silica gel at 90% RH was larger than the amount of 1‐MCP released at 0% RH. Release of 1‐MCP from paper and Tyvek sachets was largely dependent on the sorbate‐absorbing ability of the adsorbing agents.
Supplementary Figure 3 from Dual Inhibition of Class IA Phosphatidylinositol 3-Kinase and Mammalian Target of Rapamycin as a New Therapeutic Option for T-Cell Acute Lymphoblastic Leukemia (2023)

Supplementary Figure 3 from Dual Inhibition of Class IA Phosphatidylinositol 3-Kinase and Mammalian Target of Rapamycin as a New Therapeutic Option for T-Cell Acute Lymphoblastic Leukemia