gebhart/patrepeval huggingface dataset:
Symposium overview: the role of glutathione in neuroprotection and neurotoxicity (1999)

Although the cytoprotective effects of glutathione (GSH) are well established, additional roles for GSH in brain function are being identified that provide a pharmacological basis for the relationship between alterations in GSH homeostasis and the development of certain neurodegenerative processes. Thus, GSH and glutathione disulfide (GSSG) appear to play important functional roles in the central nervous system (CNS). A symposium, focussing on the emerging science of the roles of GSH in the brain, was held at the 37th annual meeting of the Society of Toxicology, with the emphasis on the role of glutathione in neuroprotection and neurotoxicity. Jean Francois Ghersi-Egea opened the symposium by describing the advances in our understanding of the role of the blood-brain and blood-cerebral spinal fluid (CSF) barriers in either limiting or facilitating the access of xenobiotics into the brain. Once within the brain, a multitude of factors will determine whether a chemical causes toxicity and at which sites such toxicity will occur. In this respect, it is becoming increasingly clear that GSH and its various conjugation enzymes are not evenly distributed throughout the brain. Martin Philbert discussed how this regional heterogeneity might provide a potential basis for the theory of differential sensitivity to neurotoxicants, in various regions of the brain. For certain chemicals, GSH provides neuroprotection, and Edward Lock discussed the selective toxicity of 2-chloropropionic acid (CPA) to the cerebellum and how its modification by modulating brain thiol status provides an example of GSH acting in neuroprotection. The sensitivity of the cerebellum to CPA may also be linked to the ability of this compound to activate a sub-type of the NMDA receptor. Thus, GSH and cysteine alone, or perhaps as conjugates with xenobiotics, may play a role in excitotoxicity via NMDA receptor activation. In contrast, certain chemicals may be converted to neurotoxicants following conjugation with GSH, and Arthur Cooper described how the pyridoxal 5'-phosphate-dependent, cysteine conjugate beta-lyases might predispose the brain to chemical injury in a GSH-dependent manner. The theme of GSH as a potential mediator of chemical-induced neurotoxicity was extended by Terrence Monks, who presented evidence for a role for GSH conjugation in (+/-)-3,4- methylenedioxyamphetamine-mediated serotonergic neurotoxicity.
ChemInform Abstract: Rapid Synthesis of a Pt1Ru1/Carbon Nanocomposite Using Microwave Irradiation: A DMFC Anode Catalyst of High Relative Performance. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
High-field, T2 reversed MRI of the hippocampus in transient global amnesia (2005)

The data indicate that transient global amnesia may not be a benign entity. Delayed neuronal loss within CA1 area of Lorente de No may represent its important sequel.
Amino acid sequence of rat .alpha.-lactalbumin: a unique .alpha.-lactalbumin (1982)

The amino acid sequence of rat alpha-lactalbumin has been determined. Unlike other alpha-lactalbumins which contain 122 or 123 amino acids, rat alpha-lactalbumin is unique in that it contains 140 amino acids. The extra amino acids are a 17 amino acid extension at the carboxyl terminus. The amino acid sequence of this extension is Gly124-Ala-Pro-Ala-Leu-Val-Val130-Pro-Ala-Leu-Asp-Gly135-Glu-Thr-Pro-Val-Pro140 . The extension is proline rich, which may contribute to the anomalous structural properties of rat alpha-lactalbumin. The amino acid sequence from residues 1 to 123 is similar to that of other alpha-lactalbumins. One possible explanation for the 17 amino acid extension is a mutation at the termination codon.
THE TOXIC FACTORS OF THE RED BLOOD CELL MEMBRANE (1988)

s of Papers to be Presented at the 48th Annual Session: American Association for the Surgery of Trauma Westin South Coast Plaza Newport Beach, California October 6–8, 1988: PDF Only
Research on Information Fusion Fault Diagnosis System Based on Fuzzy Neural Network (2008)

For limitation of fault diagnosis methods in the engineering machine's hydraulic system, a fault diagnosis method based on multi-sensor information fusion was presented. This method simultaneously had the faculty that fuzzy theory could process uncertain or inaccurate information and the self-study capability of the neural network, which effectively enhanced the fault diagnosis's technical leve1. Collecting samples of data through establishing many sensors in the scene of the hydraulic system, and getting the membership of the fault through the membership function, then through the training of fuzzy neural network by the BP algorithm to achieve exact fault diagnosis function of hydraulic braking system. By contrast the diagnosis result of an example, it indicates that using multi-sensor information fusion as fault diagnosis method is more accurate and reliable than using single information as fault diagnosis method in the hydraulic system fault diagnosis.
A Jehovah’s Witness with Acute Myeloid Leukemia Successfully Treated with an Epigenetic Drug, Azacitidine: A Clue for Development of Anti-AML Therapy Requiring Minimum Blood Transfusions (2014)

Therapy for acute leukemia in Jehovah's Witnesses patients is very challenging because of their refusal to accept blood transfusions, a fundamental supportive therapy for this disease. These patients are often denied treatment for fear of treatment-related death. We present the first Jehovah's Witness patient with acute myeloid leukemia (AML) treated successfully with azacitidine. After achieving complete remission (CR) with one course of azacitidine therapy, the patient received conventional postremission chemotherapy and remained in CR. In the case of patients who accept blood transfusions, there are reports indicating the treatment of AML patients with azacitidine. In these reports, azacitidine therapy was less toxic, including hematoxicity, compared with conventional chemotherapy. The CR rate in azacitidine-treated patients was inadequate; however, some characteristics could be useful in predicting azacitidine responders. The present case is useful for treating Jehovah's Witnesses patients with AML and provides a clue for anti-AML therapy requiring minimum blood transfusions.
Synthesis and characterization of a silyl substituted bis(indenyl) zirconium dichloride and comparison of its olefin polymerization behavior to a siloxy substituted analogue (2000)

The synthesis and characterization of rac-[ethylenebis(1-(tert-butyldimethylsilyl)-3-indenyl)]zirconium dichloride (3) is reported. The silyl substituted 3/MAO was compared to its siloxy substituted analogue (4) in ethylene homo- and in ethylene-1-hexene copolymerizations to elucidate the effect of the heteroatom on polymerization performance. The influence of monomer and cocatalyst concentration and the polymerization temperature was investigated. The oxygen between the indenyl ligand and the bulky tert-butyldimethylsilyl group in the siloxy substituted 4/MAO was found to have a positive influence on polymerization activity and copolymerization performance. © 2000 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 39: 127–133, 2001
DNA damage in human llmbal epithelial cells expanded ex vivo (2016)

Purpose Limbal stem cell deficiency, secondary to insults and diseases, may be treated by transplantation of ex vivo engineered epithelial grafts. We here present preliminary data on levels of cellular DNA damage in grafts produced in two different types of culture medium. Methods Cultures were initiated using corneo‐limbal donor tissue after removal of the central area for transplant purposes. Explants (approx. 2 × 2 mm) were positioned epithelial side down on tissue culture treated polyester membranes and expanded for 4 weeks in complex medium or in medium with human serum. Cells were dissociated using Trypsin‐EDTA (0.05%) for 30 min, the enzyme activity was further inhibited by medium and serum. The cell suspension was transferred to tubes on ice and processed using the Comet Assay. Duplicate samples from each culture were analyzed in each assay by visual scoring. Using a fluorescence microscope, 100 comets (50 from each gel) were classified into five categories, 0–4, representing increasing relative tail intensities. Summing the scores (0–4) of 100 comets therefore gives an overall score of between 0 and 400 arbitrary units. Results Preliminary data show some levels of DNA damage in cells dissociated from the grafts regardless of the type of culture medium used. Anyway more experiments with other donors have to be done to have some conclusions. Conclusions Recent studies have shown that medium with human serum equally support production of grafts containing differentiated as well as undifferentiated cells suitable for clinical transplantation. Preliminary data from our experiments indicate that levels of molecular damage to the DNA do not increase in cells cultured in human medium despite its lacks of complexity.
Cooperative Energy Trading in CoMP Systems Powered by Smart Grids (2014)

This paper studies the energy management in the coordinated multi-point (CoMP) systems powered by smart grids, where each base station (BS) with local renewable energy generation is allowed to implement the two-way energy trading with the grid. Due to the uneven renewable energy supply and communication energy demand over distributed BSs as well as the difference in the prices for their buying/selling energy from/to the gird, it is beneficial for the cooperative BSs to jointly manage their energy trading with the grid and energy consumption in CoMP based communication for reducing the total energy cost. Specifically, we consider the downlink transmission in one CoMP cluster by jointly optimizing the BSs' purchased/sold energy units from/to the grid and their cooperative transmit precoding, so as to minimize the total energy cost subject to the given quality of service (QoS) constraints for the users. First, we obtain the optimal solution to this problem by developing an algorithm based on techniques from convex optimization and the uplink-downlink duality. Next, we propose a sub-optimal solution of lower complexity than the optimal solution, where zero-forcing (ZF) based precoding is implemented at the BSs. Finally, through extensive simulations, we show the performance gain achieved by our proposed joint energy trading and communication cooperation schemes in terms of energy cost reduction, as compared to conventional schemes that separately design communication cooperation and energy trading.
Europa després de l'euro: integració económica i integració política (2000)

L'any 1999 passarà a la història per un doble motiu: és l'any de l'entrada en vigor de l'euro, i per tant de la integració monetària a Europa, un dels esdeveniments més crucials (si no el que més) en el camí de la construcció europea; però també és l'any de potser la major crisi política (tal vegada la cadira buida de De Gaulle ho va ser més) de la Comunitat, amb la dimissió de la Comissió ...
Influence of light on long-term ADP phosphorylation. (1985)

ADP phosphorylation coupled to cyclic electron transport was studied over a long period using immobilized chromatophores from Rhodopseudomonas capsulata. Photophosphorylation of ADP as a function of time was continuously measured under different conditions of continuous illumination and concentration of oxygen. Using a red cutoff filter, it was possible to sustain ADP phosphorylation at a maximal rate for more than 10 days, whereas inactivation had always been observed after 5 days when white light was used. The influence of light nature on inactivation was demonstrated using photopigment absorption spectra.
A nonhuman primate model of inherited retinal disease (2019)

Inherited retinal degenerations are a common cause of untreatable blindness worldwide, with retinitis pigmentosa and cone dystrophy affecting approximately 1 in 3500 and 1 in 10,000 individuals, respectively. A major limitation to the development of effective therapies is the lack of availability of animal models that fully replicate the human condition. Particularly for cone disorders, rodent, canine, and feline models with no true macula have substantive limitations. By contrast, the cone-rich macula of a nonhuman primate (NHP) closely mirrors that of the human retina. Consequently, well-defined NHP models of heritable retinal diseases, particularly cone disorders that are predictive of human conditions, are necessary to more efficiently advance new therapies for patients. We have identified 4 related NHPs at the California National Primate Research Center with visual impairment and findings from clinical ophthalmic examination, advanced retinal imaging, and electrophysiology consistent with achromatopsia. Genetic sequencing confirmed a homozygous R565Q missense mutation in the catalytic domain of PDE6C, a cone-specific phototransduction enzyme associated with achromatopsia in humans. Biochemical studies demonstrate that the mutant mRNA is translated into a stable protein that displays normal cellular localization but is unable to hydrolyze cyclic GMP (cGMP). This NHP model of a cone disorder will not only serve as a therapeutic testing ground for achromatopsia gene replacement, but also for optimization of gene editing in the macula and of cone cell replacement in general.
A New ECDLP-Based PoW Model (2020)

Blockchain technology has attracted a lot of research interest in the last few years. Originally, their consensus algorithm was Hashcash, which is an instance of the so-called Proof-of-Work. Nowadays, there are several competing consensus algorithms, not necessarily PoW. In this paper, we propose an alternative proof of work algorithm which is based on the solution of consecutive discrete logarithm problems over the point group of elliptic curves. At the same time, we sketch a blockchain scheme, whose consensus is reached via our algorithm. In the considered architecture, the curves are pseudorandomly determined by block creators, chosen to be cryptographically secure and changed every epoch. Given the current state of the chain and a prescribed set of transactions, the curve selection is fully rigid, therefore trust is needed neither in miners nor in the scheme proposers.
The Emergence of Institutional Repositories: A Conceptual Understanding of Key Issues through Review of Literature (2018)

It is the responsibility of the libraries to keep update its users by incorporating different technologies or tricks among the services offered to users. The libraries are managing diversified collection in both electronic and physical formats including the theses and dissertations awarded by their respective parent institutes in physical form. The academic libraries are directed by the Indian government through a mandate to protect and preserve the theses and dissertation in electronic form and provide access to the public domain. Institutional Repositories (IRs) have the perspective to store any amount of information electronically. Therefore, many of the academic libraries are forced to develop their IRs. The present paper is an attempt to find out the answers to some of the burning questions related to creation and management of IRs by the libraries. The library managers are sometimes confused when they asked to install the IRs Software, design the IR policy, motivate faculty, prepare budget estimates for IRs, etc. The current study covered various theoretic aspects related to the creation and maintenance of an IR in an academic institution. The study is based on the review of available literature and analysis focused various aspects of motivations, cost factors and software requirement and its global perspectives for the creation of Institutional Repositories. The paper also discusses the role of IRs in Scholarly Communication. Finally, it highlights the barriers for IRs in an academic environment appeared in the repository literature.
miRNA control of apoptotic programs: focus on ovarian cancer (2011)

miRNAs are a class of small non-coding RNAs that regulate the stability or translational efficiency of targeted mRNAs. miRNAs are involved in many cellular processes, such as differentiation, proliferation and apoptosis, which are altered in cancer through miRNA expression dysregulation. In this article we will discuss recent findings implicating miRNAs in apoptotic program regulation using ovarian carcinoma as an example. Ovarian cancer is the most lethal gynecological malignancy. Most patients are diagnosed with advanced disease that is conventionally managed with surgical resection followed by platinum-based chemotherapy. Killing of cancer cells by chemotherapeutic agents or by triggering cell-surface death receptors relies on activation of apoptotic programs executed through receptor-mediated extrinsic pathways and mitochondrial-dependent intrinsic pathways. Despite an initial good response to chemotherapy, ovarian cancer patients typically experience disease relapse within 2 years of the initial treatment developing resistance even to structurally different drugs. Thus, also in this pathology, tumor cells are able to evade apoptosis using multiple mechanisms, several of which are dependent on miRNA gene regulation.
Affinity Maturation Drives Epitope Spreading and Generation of Proinflammatory Anti–Citrullinated Protein Antibodies in Rheumatoid Arthritis (2018)

These findings demonstrate that established RA is characterized by a persistent IgA ACPA response that exhibits ongoing affinity maturation. This observation suggests the presence of a persistent mucosal antigen that continually promotes the production of IgA plasmablasts and their affinity maturation and epitope spreading, thus leading to the generation of ACPAs that bind additional citrullinated antigens and more potently stimulate macrophage production of TNF.
The Weak-Acid Preservative Sorbic Acid Is Decarboxylated and Detoxified by a Phenylacrylic Acid Decarboxylase, PadA1, in the Spoilage Mold Aspergillus niger (2007)

Resistance to sorbic and cinnamic acids is mediated by a phenylacrylic acid decarboxylase (PadA1) in Aspergillus niger. A. niger DeltapadA1 mutants are unable to decarboxylate sorbic and cinnamic acids, and the MIC of sorbic acid required to inhibit spore germination was reduced by approximately 50% in DeltapadA1 mutants.
Single-cell Fluorescence Resonance Energy Transfer Analysis Demonstrates That Caspase Activation during Apoptosis Is a Rapid Process (2002)

Activation of effector caspases is considered to be the final step in many apoptosis pathways. We transfected HeLa cells with a recombinant caspase substrate composed of cyan and yellow fluorescent protein and a linker peptide containing the caspase cleavage sequence DEVD, and we examined the cleavage kinetics at the single-cell level by fluorescence resonance energy transfer (FRET) analysis. Caspase activation in response to tumor necrosis factor-alpha, staurosporine, or etoposide resulted in cleavage of the linker peptide and subsequent disruption of the FRET signal. The time to caspase activation varied among individual cells, depending on the type of treatment and concentration used. However, once initiated, disruption of the FRET signal was always rapid (<or=15 min) and largely independent of these parameters. In contrast, FRET probe cleavage was significantly slower in the caspase-3-deficient MCF-7 cells, particularly at low concentrations of the pro-apoptotic agents. Under these conditions, MCF-7 cells required up to 90 min for the FRET probe cleavage, whereas MCF-7/Casp-3 cells displayed rapid cleavage kinetics. Interestingly, we could still observe comparable cell death rates in MCF-7 and MCF-7/Casp-3 cells. Our results suggest that caspase activation during apoptosis occurs in an "all or nothing" fashion. Caspase-3 is required for rapid cleavage kinetics when the onset of apoptosis is slow, suggesting the existence of caspase-3-dependent feedback loops.
The Boston Ocular Surface Prosthesis as a Novel Drug Delivery System for Bevacizumab (2009)

Corneal neovascularization causes deterioration of visual acuity and increases surface irregularities. Various techniques have been employed to help control the progression of corneal neovascularization; bevacizumab is a medication that targets the specific pathway of corneal neovascularization. The Boston Ocular Surface Prosthesis (BOSP) is a large diameter contact lens that aids in maintaining corneal surface integrity and may serve as a delivery system for topical bevacizumab. This paper reviews five patients who were treated with topical bevacizumab in their BOSP. All patients demonstrated improvement in their visual acuity and clinical exam. No adverse reactions were noted.
Open Challenges in Protein Complex Prediction (2017)

Chapter 5 Share on Open Challenges in Protein Complex Prediction Authors: Sriganesh Srihari South Australian Health and Medical Research Institute (SAHMRI) South Australian Health and Medical Research Institute (SAHMRI)View Profile , Chern Han Yong Duke-National University of Singapore Medical School Duke-National University of Singapore Medical SchoolView Profile , Limsoon Wong National University of Singapore National University of SingaporeView Profile Authors Info & Claims Computational Prediction of Protein Complexes from Protein Interaction NetworksMay 2017 https://doi.org/10.1145/3064650.3064656Published:27 February 2018Publication History 0citation12DownloadsMetricsTotal Citations0Total Downloads12Last 12 Months1Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Longitudinal linked-read sequencing reveals ecological and evolutionary responses of a human gut microbiome during antibiotic treatment (2021)

Gut microbial communities can respond to antibiotic perturbations by rapidly altering their taxonomic and functional composition. However, little is known about the strain-level processes that drive this collective response. Here, we characterize the gut microbiome of a single individual at high temporal and genetic resolution through a period of health, disease, antibiotic treatment, and recovery. We used deep, linked-read metagenomic sequencing to track the longitudinal trajectories of thousands of single nucleotide variants within 36 species, which allowed us to contrast these genetic dynamics with the ecological fluctuations at the species level. We found that antibiotics can drive rapid shifts in the genetic composition of individual species, often involving incomplete genome-wide sweeps of pre-existing variants. These genetic changes were frequently observed in species without obvious changes in species abundance, emphasizing the importance of monitoring diversity below the species level. We also found that many sweeping variants quickly reverted to their baseline levels once antibiotic treatment had concluded, demonstrating that the ecological resilience of the microbiota can sometimes extend all the way down to the genetic level. Our results provide new insights into the population genetic forces that shape individual microbiomes on therapeutically relevant timescales, with potential implications for personalized health and disease.