gebhart/patrepeval huggingface dataset:
Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient Neurons (2023)

Progranulin (PGRN) is a lysosomal protein implicated in various neurodegenerative diseases. Over 70 mutations discovered in the <i>GRN</i> gene all result in reduced expression of PGRN protein. However, the detailed molecular function of PGRN within lysosomes and the impact of PGRN deficiency on lysosomal biology remain unclear. Here we leveraged multifaceted proteomic techniques to comprehensively characterize how PGRN deficiency changes the molecular and functional landscape of neuronal lysosomes. Using lysosome proximity labeling and immuno-purification of intact lysosomes, we characterized lysosome compositions and interactomes in both human induced pluripotent stem cell (iPSC)-derived glutamatergic neurons (i<sup>3</sup>Neurons) and mouse brains. Using dynamic stable isotope labeling by amino acids in cell culture (dSILAC) proteomics, we measured global protein half-lives in i<sup>3</sup>Neurons for the first time and characterized the impact of progranulin deficiency on neuronal proteostasis. Together, this study indicated that PGRN loss impairs the lysosome's degradative capacity with increased levels of v-ATPase subunits on the lysosome membrane, increased catabolic enzymes within the lysosome, elevated lysosomal pH, and pronounced alterations in neuron protein turnover. Collectively, these results suggested PGRN as a critical regulator of lysosomal pH and degradative capacity, which in turn influences global proteostasis in neurons. The multi-modal techniques developed here also provided useful data resources and tools to study the highly dynamic lysosome biology in neurons.
A troposcatter propagation experiment at 15.7 GHz over a 500 km path (1968)

An experiment to study tropospheric structure through the use of high resolution antennas having a very small common volume is described. Measurements at 15.7 GHz over a 500 km path between Boston and Ottawa have been conducted, and signals as large as 25 dB above the receiver noise level have been obtained.
Deep sponge communities of the Gulf of St Eufemia (Calabria, southern Tyrrhenian Sea), with description of two new species (2013)

Recently, the rich coral communities of the so called roche du large biocoenose of the Gulf of St Eufemia (southern Tyrrhenian Sea) between 90 and 130 m deep, have been described thanks to remotely operated vehicle (ROV) imaging. This preliminary survey evidenced the massive presence of a well-diversified sponge community living among the coral colonies. This work aims at giving an ecological overview of some of the dominant species of this environment, together with a taxonomic part including the description of new species: Topsentia calabrisellae sp. nov. and Haliclona (Soestella) fimbriata sp. nov. The silted, rocky outcrops of the Gulf of St Eufemia facilitate the settling mainly of massive sponges with erect habit which may also avoid sedimentation by growing on the coral colonies. On the other hand, the site Capo Vaticano, located at the southern boundary of the gulf, characterized by rocky boulders exposed to strong currents and completely devoid of corals, is inhabited by a poor megabenthic community, dominated by patches of massive sponges, such as Topsentia vaceleti , a species of recent description whose aspect in the field was still unknown. In the entire investigated area 19 species have been photographed (often for the first time in vivo ), collected and identified, but the real sponge diversity is certainly under-estimated due to the difficulty of collecting all the sighted specimens through the ROV grabber. Similarly to the coral component, sponges also respond to the same environmental constraints by growing in heterogeneous patches or by showing different morphologies mainly associated with current and sedimentation conditions.
R5 High Temperature Creep-Fatigue Life Assessment for Austenitic Weldments (2014)

This paper presents an overview of the R5 high temperature creep-fatigue procedure for austenitic materials. In particular revision to the weld procedures to be incorporated in the upcoming version of R5 are discussed in detail. The main feature of the new approach is that the traditional fatigue strength reduction factor (FSRF) which is applied to both creep and fatigue strain predictions is separated into a local weld strain enhancement factor (WSEF) and weld fatigue endurance reduction (WER). The new method is applied to the example of a fillet welded attachment on a boiler tube where a significant increase in predicted life is illustrated by adopting the new method new method. The principal advantage stems from a reduction in the predicted stress at the start of creep dwells and hence the level of creep damage in a cycle.
Effects of Adenosine Deaminase Inhibition on Blood Pressure in Old Spontaneously Hypertensive Rats (1998)

Adenosine is an ubiquitously occurring endogenous nucleoside that via cell surface receptors exerts multiple antihypertensive actions, and mediates a number of biological responses that may reduce cardiovascular disease risk. Therefore modulation of endogenous levels of adenosine may offer beneficial effects in hypertension. The objective of this study was to determine whether inhibition of adenosine deaminase lowers blood pressure in spontaneously hypertensive rats (SHR). We investigated the effects of erythro-9-(2-hydroxyl-3-nonyl) adenine (EHNA), an adenosine deaminase inhibitor, on hemodynamic and renal parameters in 16-week-old and 36-week-old SHR and normotensive Wistar Kyoto rats (WKY) and in 36-week-old SHR and WKY pretreated with 1,3-dipropyl-8-p-sulfopheznylxanthine (DPSPX, an adenosine antagonist that does not enter the brain and is restricted to the extracellular space). Adenosine deaminase inhibition with EHNA (10 mg/kg, iv.) produced a marked fall in arterial blood pressure in older (MABP 162.0+/-7.6 mmHg and 120.7+/-11.7 mmHg for baseline and EHNA period, respectively; p<0.01), but not younger, SHR, whereas no effects on blood pressure were observed in age-matched normotensive WKY rats. EHNA did not affect renal hemodynamic and excretory function in any of six groups of animals. DPSPX blocked the antihypertensive effects of EHNA, suggesting that the effects of EHNA on blood pressure are mediated via peripheral adenosine receptors. Further studies are required to elucidate why inhibition of adenosine deaminase lowers blood pressure only in older SHR. The present data suggest that inhibition of adenosine deaminase may provide beneficial effects in older hypertensives and lead us to propose that design and use of extracellular adenosine deaminase inhibitors may offer cardiovascular protection in hypertension.
[High expression of human EPO gene in the larvae and pupae of the silkworm, Bombyx mori]. (2000)

Erythropoietin (EPO) is a glycoprotein hormone produced primarily by the kidney, and is principal factor regulating red blood cell poduction. The synthesized EPO cDNA was inserted into the transfer vector pBlueBac III to generate the recombinant transfer plasmid pBlueBacEPO. Cotransfection of BmN cells with pBlueBacEPO DNA and Wild BmNPV DNA generated the recombinant virus rBmNPVEPO carrying EPO gene driven by the strong promoter of AcNPV polyhedrin gene. The results of Southern blot and PCR reaction confirmed that EPO gene had been correctly inserted in the target position in the BmNPV genome. ELISA assay showed that the EPO gene was expressed with high level in the larvae and pupae of the silkworm. The larvae and pupae produced as high as 62,800 u and 74,000 u in 1 mL hemolymph on the 4th day (larve) and the 5th day(pupae) after infection with the recombinant virus rBmNPVEPO, respectively. Western blot analysis showed the molecular weight of rhEPO produced in the lavae or pupae was about 26 kD. Biologic assay showed the rhEPO had high activity (about 63,000 u in per milliliter of hemolymph) in vitro.
Ethanol‐extracted soy protein isolate results in elevation of serum cholesterol in exogenously hypercholesterolemic rats (1999)

Soy protein preparations were reported to have hypocholesterolemic actions in experimental animals and humans, while the active components and the mechanism by which this occurs are not clarified yet. The objective of this study is to address these issues by using exogenously hypercholesterolemic rats which are susceptible to dietary cholesterol. Two groups of five rats (male, 12-wk-old) were fed on AIN 93G-based diet with soy protein isolate (SPI) or ethanol-extracted SPI (EE-SPI) for 2 wk. EE-SPI was prepared by ethanol extraction to remove isoflavones and other components. Concentrations of serum and liver total cholesterol were lower in rats fed SPI than in those fed EE-SPI. The abundances of mRNA for 7alpha-hydroxylase and low density lipoprotein receptor in the liver were lower in EE-SPI group than those in SPI group. These results suggest that the ethanol extract from SPI has a factor(s) to alleviate hypercholesterolemia by increasing the removal of cholesterol from serum through the receptor pathway and then from liver through enhancement of bile acid synthesis.
THE TURVILLE INFINITY BINOCULAR BALANCE TEST (1949)

School of Optometry, University of California Berkeley, California *Read before the annual meeting of the American Academy of Optometry, Winston-Salem, North Carolina, December 5, 1948. For publication in the June, 1949, issue of the American Journal of Optometry and Archives of American Academy of Optometry. †Optometrist. Fellow, American Academy of Optometry. Assistant Professor of Optometry.
Tuning the Fano resonance between localized and propagating surface\n plasmon resonances for refractive index sensing applications (2012)

Localized and propagating surface plasmon resonances are known to show very\npronounced interactions if they are simultaneously excited in the same\nnanostructure. Here we study the fano interference that occurs between\nlocalized (LSPR) and propagating (SPP) modes by means of phase sensitive\nspectroscopic ellipsometry. The sample structures consist of periodic gratings\nof gold nanodisks on top of a continuous gold layer and a thin dielectric\nspacer, in which the structural dimensions were tuned in such a way that the\ndipolar LSPR mode and the propagating SPP modes are excited in the same\nspectral region. We observe pronounced anti-crossing and strongly asymmetric\nline shapes when both modes move to each others vicinity, accompagnied of\nlargely increased phase differences between the respective plasmon resonances.\nMoreover we show that the anti-crossing can be exploited to increase the\nrefractive index sensitivity of the localized modes dramatically, which result\nin largely increased values for the Figure-Of-Merit which reaches values\nbetween 24 and 58 for the respective plasmon modes.\n
Thermal aging performance of glass fiber/polyphenylene sulfide composites in high temperature (2021)

Abstract In order to use the glass fiber reinforced polyphenylene sulfide composites (GF/PPS) in high temperature environments, thermal aging performance of two kinds of commercial grade PPS composites, reinforced by 40% glass fiber, PPS‐G40 HM and 1140L4, in thermal aging temperature of 250°C was compared by tensile strength, oxidized layer, color, crystallization and melting behavior. The results showed that tensile strength of GF/PPS composites is significantly decreased with increasing of aging time below 200 h and the tensile strength of aged PPS‐G40 HM is higher than that of aged 1140L4. The thickness of dark color area is increased with increasing of aging time. The thickness of oxidized layer of 1140L4 is thinner than that of PPS‐G40 HM. However, the color of oxidized layer of PPS‐G40 HM is lighter than that of 1140L4. The recrystallization in thermal aging results in the formation of crystal with higher melting point and increased melting temperature of GF/PPS composites. It is found that addition of epoxy resin can increase the initial mechanical property and improve the thermal aging performance of GF/PPS composites. A novel modified GF/PPS composite with higher thermal aging properties was obtained.
Reduced risk of colon cancer with high intake of vitamin E: the Iowa Women's Health Study. (1993)

Antioxidant micronutrients, including vitamin E, vitamin C, the carotenoids, and selenium, defend the body against free radicals and reactive oxygen molecules, suggesting a potential for these dietary components in cancer prevention. To investigate whether high intakes of antioxidant micronutrients protect against colon cancer in humans, we analyzed data from a prospective cohort study of 35,215 Iowa women aged 55-69 years and without a history of cancer who completed a dietary questionnaire in 1986. Through 1990, 212 incident cases of colon cancer were documented. Adjusted for age, total vitamin E intake was inversely associated with the risk of colon cancer (P for trend < 0.0001); the relative risk for the highest compared to the lowest quintile was 0.32 [95% confidence interval (95% CI) 0.19, 0.54]. Further adjustment for total energy intake and other risk factors in proportional hazards regression had little effect on these estimates. The association was not uniform across age groups: the multivariate relative risk of colon cancer for the highest compared to the lowest quintile of total vitamin E intake was 0.16 (95% CI 0.04, 0.70) for those 55-59 years old, 0.37 (95% CI 0.12, 1.16) for those 60-64 years old, and 0.93 (95% CI 0.27, 3.25) for those 65-69 years old. Multivariate-adjusted relative risks among women with higher total intakes of vitamins A and C and beta-carotene, and among users of selenium supplements, were not significantly different from 1.0. These prospective data provide evidence that a high intake of vitamin E may decrease the risk of colon cancer, especially in persons under 65 years of age.
RoboRay hand: A highly backdrivable robotic hand with sensorless contact force measurements (2014)

This paper presents a 14-DOF robotic hand including 5 fingers and a wrist. The hand has a new tendon-driven mechanism which minimizes frictional loss and maximizes efficiency and backdrivability. In order to accomplish high efficiency and backdrivability as well as human-like payload and dexterity in a compact size, two novel mechanical concepts are proposed. Firstly, the actuators are placed according to the functions of fingers - high power grasping and precise manipulation - instead of positioning at each joint. For the high power grasping, 7 high payload motors are positioned in the forearm, and 5 small size motors are positioned in the palm for the precise manipulation. Secondly, a new tension decoupling mechanism is proposed to the 2-DOF wrist joint, which delivers wire motions of the forearm motors to the fingers without frictional loss or coupling with wrist motion. A total weight including the forearm is 1.59kg which is similar to human. The fingertip force is 15N which is sufficient for most of household work. High backdrivability enables the contact force sensing by measuring a motor current without additional sensors. A detectable minimum contact force was 0.735N. In order to enhance the contact force sensing capability, a friction compensation algorithm was applied, which resulted to the minimum contact force as 0.196N. Theoretical and experimental analyses are also performed.
Bioequivalence of over-the-counter (OTC) progesterone cream (PC) (2004)

Progesterone cream is an OTC (non-prescription) product that contains USP Progesterone (P). The exposure (E) of progesterone cream (PC) was compared to an FDA approved oral micronized form of P. Twelve healthy postmenopausal (as determined by elevated FSH and menstrual cycle history) Caucasian females, mean age of 54.6+7.0 yr and mean weight of 77.2+16.6 kg were enrolled. The trial consisted of 2 phases: women received PC (Pro-gest® Transitions for Health Inc., Portland, OR) 40 mg twice daily topically for 12 days using rotating sites (phase I) or oral micronized P (Prometrium® Solvay Pharmaceuticals, Marietta, GA) 200 mg daily for twelve days (phase II) in a randomized, crossover fashion. Dosing was designed to achieve steady-state concentrations. A washout period of 1 month between phases was used. On day 12 of each phase, whole blood and saliva for P were collected at 0, 2, 4, 6, 8, 10, and 12 hrs after final PC application and at 0,1, 2, 4, 6, 10, 12, and 24 hrs after last ingestion of oral P. Salivary (reflecting free drug) and whole blood progesterone concentrations were measured using LC-MS-MS. WinNonlin was used to determine the AUC0–12 hr for PC (normalized to an 80 mg/24 hr total dose) and AUC0-24 hr for oral P using non-compartmental analysis. A paired t-test was used to compare AUC0-24hr for the 80 mg daily dose of PC to the 200 mg oral P.(See Table) OTC PC at a dose of 40 mg twice daily gives equal exposure to oral P at a dose of 200 mg daily. This is presumably due to the high first pass metabolism of oral P that should not occur with PC. This raises the question of whether OTC PC should be regulated since it may provide significant exposure compared to FDA approved products. Clinical Pharmacology & Therapeutics (2004) 75, P10–P10; doi: 10.1016/j.clpt.2003.11.036 P Form Adjusted AUC(ng-hr/ml) [mean(90% CI)] p value PC (Pro-gest®) 17.0 (9.9–24) 0.874 Oral P (Prometrium®) 16.2 (8.4–24)
Detection of JC virus DNA sequences and expression of viral T antigen and agnoprotein in esophageal carcinoma (2005)

The results provide evidence for infection of gastrointestinal tract cells by JCV and suggest a potential role of JCV in the development of upper digestive tract carcinomas.
Modulation of the plasma emerging from a duoplasmatron (1981)

The output of hydrogen plasma emerging from a duoplasmatron was modulated by modulating the are current in the anode-intermediate electrode gap. It could be decreased, or increased, by 20–30% from its steady state value for a duration of few microseconds. The plasma pulses were detected downstream, and their time of flight few measured; an estimate of plasma velocity was made. The effect of the gas pressure on the measured velocity was also investigated.
The Hyperthermia-enhanced Association between Tropoelastin and Its 67-kDa Chaperone Results in Better Deposition of Elastic Fibers (2010)

The results of our in vitro experiments indicate that exposing cultured human aortic smooth muscle cells and dermal fibroblasts to 39 to 41 °C induces a significant up-regulation in the net deposition of elastic fibers, but not of collagen I or fibronectin, and also decreases the deposition of chondroitin sulfate-containing moieties. We further demonstrate that mild hyperthermia also rectifies the insufficient elastogenesis notable in cultures of fibroblasts derived from the stretch-marked skin of adult patients and in cultures of dermal fibroblasts from children with Costello syndrome, which is characterized by the accumulation of chondroitin 6-sulfate glycosaminoglycans that induce shedding and inactivation of the 67-kDa elastin-binding protein. We have previously established that this protein serves as a reusable chaperone for tropoelastin and that its recycling is essential for the normal deposition of elastic fibers. We now report that hyperthermia not only inhibits deposition of chondroitin 6-sulfate moieties and the consequent preservation of elastin-binding protein molecules but also induces their faster recycling. This, in turn, triggers a more efficient preservation of tropoelastin, enhancement of its secretion and extracellular assembly into elastic fibers. The presented results encourage using mild hyperthermia to restore elastic fiber production in damaged adult skin and to enhance elastogenesis in children with genetic elastinopathies.
Improving signal quality of a speech codec using hybrid perceptual-parametric algorithm (2008)

A hybrid parametric-perceptual speech codec architecture is presented. The basic CELP parametric codec structure is enhanced using the perceptual coding method. The objective of the codec hybridisation is obtaining significant improvement in the perceived signal quality. Two hybrid architectures are proposed. The first one encodes voiced parts perceptually in the CELP residual signal. The second one divides the signal into voiced signal components that are encoded using the perceptual algorithm, unvoiced components that are encoded parametrically and transients remaining unencoded. The signal quality achieved using the hybrid codec is compared to the quality of some standard speech codecs.
Youtube traffic characterization (2007)

This paper presents a traffic characterization study of the popular video sharing service, YouTube. Over a three month period we observed almost 25 million transactions between users on an edge network and YouTube, including more than 600,000 video downloads. We also monitored the globally popular videos over this period of time.
Efficiency of virtual surgical planning in management of maxillofacial trauma (2023)

Background: Craniomaxillofacial (CMF) injuries has different patterns from simple to complicated ones. Surgeons usually spend time and effort planning the optimal surgical approach to avoid damage to vital structures that could otherwise lead to functional deficits. It is also imperative to optimize the skin incisions to avoid unfavourable scarring. The aim of this work is to assess the effectiveness and efficiency of virtual surgical planning (VSP) application in management of different types of CMF traumas, the advantages, and disadvantages of VSP, and the accuracy of three dimensional (3D) measures as a preoperative planning tool.Methods: This prospective consecutive series study enrolled 30 patients presented with different types of CMF trauma. All patients were subjected to CT scans. The obtained Digital imaging and communications in Medicine (DICOM) files were imported into the software for virtual planning.Results: The actual operative time (OT) after VSP was significantly lower than the expected time by traditional methods (p=0.008). There was no significant difference between the actual and expected number of incisions. The accuracy measures of preoperative VSP were very close postoperatively. The functional, anatomic reduction and patient satisfaction outcomes were very satisfying to the surgeon and the patient respectively. Conclusions: VSP can be a valuable tool for optimizing functional and aesthetic outcomes of the CMF trauma surgery. The accurate measurements obtained pre-operatively helped in reducing surgical time and minimizing the need for subsequent revision surgeries.