gebhart/patrepeval huggingface dataset:
The Synthesis and Secretion of γ-Globulin by Lymph Node Cells (1969)

Microsomes derived from guinea pigs immunized with dinitrophenyl-protein conjugates possess firmly associated antibodies specific for the dinitrophenyl determinant. The fact that these antibodies can be specifically displaced by incubation with an excess of soluble, normal γ-globulins provides a method for studying the site (sites) on γ-globulin involved in the microsome-antibody complex. After digestion of γ-globulin or of the Fc fragment, several peptide fractions were separated by molecular sieve chromatography. All of these were found to be capable of solubilizing antibody from the microsomes. The peptides in one of the active fractions appear to be quite small as judged by their absorption into the matrix of Sephadex G-25 and G-15. The effect of mild periodate oxidation of γ-globulin on its ability to dissociate antibody from the microsomes was also examined. After oxidation, the ability of γ-globulin to displace antibody was increased 5-fold. In contrast, the reduction of a single disulfide bond located at the center of the heavy chains of γ-globulin resulted in a 5-fold decrease in the capacity of such γ-globulin to dissociate antibody. Direct reduction of the microsome-antibody complex also resulted in a loss of microsomal antibody. These findings and the fact that the reactive disulfide is adjacent to a threonine residue bearing one of the carbohydrate chains of γ-globulin are discussed in relation to the nature of the antibody-binding site on microsomes.
Dependence of particulate sampling efficiency on inlet orientation and flow velocities (1982)

Abstract The function of the inlet of a particulate sampler is to capture particles in the air and duct them to a collection filter or a dynamic sensor. For a given inlet the aerosol concentration and size distribution registered by the sampler may be strongly modified by the orientation of the inlet, θ, relative to the ambient wind direction, and by the velocity ratio, R, defined as the ratio of wind velocity to inlet velocity. We have studied such dependence with a thin-walled inlet tube at wind velocities of 250 to 1000 cm/s, inlet velocities of 125 to 1000 cm/s, and angles of 0° to ±90°. For all the tested velocity-combinations differences in sampling efficiency were found for particles above 10 µm in diameter when the aerosol was sampled 15° upward vs. 15° downward from the horizontal. For θ=30 to 90° the sampling efficiency was found to be approximately a function of Stokes number with R as a parameter. At θ=90° the sampling efficiency was approximately a function of Stk . About half or more of the particle deposition in the inlet occurred within the first 1 cm of the 20 cm long inlet tube.
Catalase inhibition alters suberization and wound healing in potato (Solanum tuberosum) tubers (2007)

In response to wounding, potato ( Solanum tuberosum L.) tubers generate hydrogen peroxide (H 2 O 2 ) in association with suberization, a critical phase of the wound‐healing process. In the present study, the effect of aminotriazole (AT), a catalase (CAT, EC 1.11.1.6) inhibitor, on cut tubers was investigated using fresh weight (FW) loss and pathogen attack symptoms as indicators of wound‐healing efficiency. Seven days after treatment, AT‐treated tuber halves lost more FW and developed infection signs compared with the controls. Thiourea, another CAT inhibitor, as well as exogenous H 2 O 2 treatments induced the same effects as AT suggesting that the alteration of the wound healing may be caused by CAT inhibition and the resulting accumulation of H 2 O 2 . Using transgenic tubers, FW losses 1 week after wounding were either higher (CAT repression) or lower (CAT overexpression) than those of the wild‐type. When tuber halves were allowed to wound heal for different periods before treatment, AT had no effect on the progress of their wound healing if wound‐healed for at least 3 days. This implies that AT may affect early wound‐healing‐related events, especially those occurring before or during suberization. A time‐course analysis of the effects of AT treatment on wounded tuber tissues revealed that AT prevented the deposition of the polyphenolic domain of suberin in association with CAT inhibition and H 2 O 2 accumulation. These data are important in identifying factors that may be required to regulate suberization and contribute to a better understanding of this critical process to hasten its rate and limit wound‐related losses in stored potato tubers.
Radio-frequency response of single pores and artificial ion channels (2011)

Intercellular communication relies on ion channels and pores in cell membranes. These protein-formed channels enable the exchange of ions and small molecules to electrically and/or chemically interact with the cells. Traditionally, recordings on single-ion channels and pores are performed in the dc regime, due to the extremely high impedance of these molecular junctions. This paper is intended as an introduction to radio-frequency (RF) recordings of single-molecule junctions in bilipid membranes. First, we demonstrate how early approaches to using microwave circuitry as readout devices for ion channel formation were realized. The second step will then focus on how to engineer microwave coupling into the high-impedance channel by making use of bio-compatible micro-coaxial lines. We then demonstrate integration of an ultra-broadband microwave circuit for the direct sampling of single α-hemolysin pores in a suspended bilipid membrane. Simultaneous direct current recordings reveal that we can monitor and correlate the RF transmission signal. This enables us to relate the open–close states of the direct current to the RF signal. Altogether, our experiments lay the ground for an RF-readout technique to perform real-time in vitro recordings of pores. The technique thus holds great promise for research and drug screening applications. The possible enhancement of sampling rates of single channels and pores by the large recording bandwidth will allow us to track the passage of single ions.
Differential subunit dependence of the actions of the general anesthetics alphaxalone and etomidate at gamma-aminobutyric acid type A receptors expressed in Xenopus laevis oocytes. (1997)

The effects of subunit composition of the gamma-aminobutyric acid (GABA) type A receptor on the multiple actions of the general anesthetics alphaxalone and etomidate were investigated. The abilities of the two drugs to activate directly Cl- currents and to modulate GABA-evoked Cl- currents mediated by human recombinant GABA(A) receptors composed of alpha1, gamma2S, and either beta1, beta2, or beta3 subunit expressed in Xenopus laevis oocytes were compared. Both alphaxalone and etomidate evoked Cl currents in alpha1 beta1 gamma2S, alpha1 beta2 gamma2S, and alpha1 beta3 gamma2S receptors, an action that was blocked by both SR 95531 and picrotoxin. However, although maximal current activation by alphaxalone varied only slightly with the specific beta subunit isoform present, the efficacy of etomidate showed a rank order of beta3 > beta2 >>> beta1. In addition, beta1 homomeric receptors were markedly activated by etomidate but not by alphaxalone. Conversely, receptors consisting of alpha1 and gamma2S subunits were markedly activated by alphaxalone but not by etomidate. The modulatory effect of alphaxalone was also not markedly influenced by the beta-specific subunit isoform, whereas the modulatory efficacy of etomidate showed a rank order of beta3 > beta2 >> beta1. These results further demonstrate that the actions of general anesthetics at GABA(A) receptors are influenced by receptor subunit composition, and they suggest that the effects of alphaxalone and etomidate are mediated by different binding sites on the receptor complex.
Into the Woods: Experiences from Building a Dataflow Analysis Framework for C/C++ (2021)

Welcome to the 21st IEEE International Working Conference on Source Code Analysis and Manipulation (SCAM 2021)! We hope that you enjoy the conference and that it will inspire you in your future research. Should you require a certificate of participation please contact Alexander Serebrenik, the general chair of SCAM, at a.serebrenik@tue.nl. For questions related to the Morressier virtual platform please contact support@morressier.com.
Quantitative aspects of chlamydial infection of the cervix. (1980)

In 580 women with Chlamydia trachonmatis infection of the cervix, the degree of the infection was assessed by counting the number of chlamydial inclusions which developed in McCoy cell monolayers inoculated with cervical swab material under standardised conditions. In 34% of these women inclusion counts were <100 per monolayer whereas in 36% the counts were greater than or equal to 1000. Clinical features in each of these groups were compared to see if certain factors could be identified as the cause, or the result, of high-grade rather than low-grade infection. A significant association was found between high inclusion counts and the presence of cervical mucopus or cervical ectopy. Oral contraceptives acted additively with ectopy but had no significant effect alone. Concurrent gonorrhoea did not affect the degree of chlamydial infection. High inclusion counts were more common in women under 20 years of age than in older women.
Synthesis and characterization of carbynoid structures in cluster-assembled carbon films (2005)

The combination of sp3, sp2 and sp hybridized atoms can give rise to a large
number of carbon allotropic forms and phases, but only carbon solids based
on all sp3 (diamond) and sp2 (graphite, fullerene) hybridization are well
known and characterized [1]. In addition there are innumerable transitional
forms of carbon where sp2 and sp3 hybridization bonds co-exist in the samesolid such as in amorphous carbon, carbon black, soot, cokes, glassy carbon
etc [2-4].
The Development of a Geographic Information System (GIS) to Document Research in an Everglades Physical Model (2011)

The Loxahatchee Impoundment Landscape Assessment (LILA) facility is a unique physical model of the Everglades ecosystem. LILA has a closed-loop water delivery system and consists of four 0.08 square kilometer (∼8 ha) macrocosms, created to be replicates of one another and of the Everglades landscape. Built in 2003, LILA's purpose is to provide scientists with an opportunity to design and implement research concerning Everglades restoration techniques in an accessible, controlled and replicated Everglades environment. Key Everglades habitats were sculpted within LILA: tree islands, ridges, sloughs and alligator holes. Water levels and flows in each macrocosm are controlled independently, so that researchers can study the effects of hydrology on Everglades landscape and ecology. Studies have focused upon measuring survival and growth of native trees planted on the tree islands; measuring surface water and ground water movement and chemistry; studying wading bird feeding and the movement of prey species (crayfish); and measuring erosion and accretion on tree islands and ridges. We developed a Geographic Information System (GIS) data set to identify, characterize, and spatially reference the features of LILA and document research activities. This development included mapping the boundaries of the landscape features, creating a theoretical Digital Elevation Model (DEM) and describing the research projects being carried out. The creation of this GIS data set enhances the ability to schedule and coordinate research, assist scientists in the visualization and spatial representation of their research, and provide a resource for the storage, analysis and synthesis of valuable scientific information.
Identification of high noise time series signals using hybrid ARMA modeling and neural network approach (2002)

A novel approach for time series signal identification with high noise background is proposed. The approach takes advantage of both the autoregressive moving average (ARMA) spectrum estimator and artificial neural networks (ANNs). The dynamic data system (DDS) modeling strategy and ARMA spectrum estimator are used to provide a high resolution spectrum estimate, and the backpropagation ANN is used as the feature pattern classifier. Simulation experiments based on vibration signal diagnosis are presented. The approach demonstrates a better performance than the conventional fast Fourier transform (FFT)-ANN approach in high noise environments.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
ChemInform Abstract: Synthesis and Application of Imidazole Derivatives. Synthesis and Acyl Activation of 2‐Acyl‐1‐methyl‐1H‐imidazoles. (1987)

Abstract Treatment of the 1‐acylpyrrolidines (I) with 2‐lithio‐1‐methylimidazole (II) yields the 2‐acylimidazoles (III).
Thermally Stable Homogeneous Catalysts for Alkane Dehydrogenation S.O. thanks the German Academic Exchange Service (DAAD) for financing a research stay with W.C.K. in the USA. This work was supported by the National Science Foundation (CHE 9800184 to M.B.H.), by the University of California Energy Institute and University of California Santa Barbara (to W.C.K.), and by the German Research Association (DFG, to M.W.H). We thank Dr. R. Mynott and Mrs. C. Wirtz, MPI für Kohlenforschung, for NMR spectroscopic investigations. (2001)

Thermostability is “conditio sine qua non” (a necessity) if organometallic compounds are used in the homogeneous catalysis of endothermic processes such as the dehydrogenation of alkanes to alkenes and hydrogen (see scheme). This thermostability and other requirements for such a catalyst are fulfilled by the anthraphos iridium(III)dihydride complex 1.
Dynamic behavior of a heated meniscus subjected to a transient acceleration field for a small capillary number (1995)

The dynamic response of a heated meniscus within a capillary tube subjected to single and multiple cycle transient acceleration induced forces was studied experimentally and analytically. A capillary tube was partially filled with ethyl alcohol and mounted on a centrifuge for visual observation of the dynamic response of the meniscus subject to an acceleration transient. A one-dimensional mathematical model was formulated to predict the dynamic response of the meniscus and compared with experimental results. For the case in which the meniscus was heated, thermocapillary stresses in the near contact line region were accounted for in the model by an effective change in contact angle to account for adverse thermocapillary stresses present at the contact line. Experimental results and analytical calculations were compared for a Bond number of 1.47 and a capillary number of 3.00 {times} 10{sup {minus}6}. Experimental data of the meniscus recession and advance agreed well with a one-dimensional equation of motion accounting for the ``dynamic`` contact angle.
Single-nucleus transcriptomics reveals functional compartmentalization in syncytial skeletal muscle cells (2020)

Abstract Syncytial skeletal muscle cells contain hundreds of nuclei in a shared cytoplasm. We investigated nuclear heterogeneity and transcriptional dynamics in the uninjured and regenerating muscle using single-nucleus RNA-sequencing (snRNAseq) of isolated nuclei from muscle fibers. This revealed distinct nuclear subtypes unrelated to fiber type diversity, completely novel subtypes as well as the expected ones at the neuromuscular and myotendinous junctions. In fibers of the Mdx dystrophy mouse model, new subtypes emerged, among them nuclei expressing a repair signature that were also abundant in the muscle of dystrophy patients, and a nuclear population associated with necrotic fibers. Finally, modifications of our approach revealed the compartmentalization in the rare and specialized muscle spindle. Our data identifies new nuclear compartments of the myofiber and defines a molecular roadmap for their functional analyses; the data can be freely explored on the MyoExplorer server ( https://shiny.mdc-berlin.net/MyoExplorer/ ).
Steady increase in cellular HIV-1 load during the asymptomatic phase of untreated infection despite stable plasma viremia (2010)

During the asymptomatic phase of untreated HIV-1 infection, when virion production and clearance are balanced, resulting in stable plasma viremia, viral load in PBMCs steadily increases and is a sensitive and direct longitudinal virological marker of infection progression.
An analysis of the operation and SET robustness of a CMOS pulse stretching circuit (2017)

The cascaded asymmetrically sized inverters can be employed as pulse stretchers, for the measurement of very short single event transient (SET) pulse widths (<; 200 ps). This paper analyzes, through the circuit simulations, the effects of various design and operating parameters on the normal operation and SET robustness of a two-inverter pulse stretcher designed in 250 nm bulk CMOS technology. It was shown that the SET hardness of the pulse stretcher can be enhanced by upsizing all transistors in the pulse stretcher without changing the sizing ratio. The SET hardness can also be improved by upsizing the load, but this approach is less effective than the pulse stretcher upsizing. Both upsizing approaches have a negligible impact on the normal operation of the stretcher, i.e. output pulse width. In addition, the operation and SET robustness of the pulse stretcher can be influenced by the operating temperature and supply voltage variations, and these effects should be considered in the design process. Based on the acquired simulation results, a general approach for the design of a radiation hardened CMOS pulse stretcher has been proposed.
[Irinotecan plus oral S-1 in patients with advanced colorectal cancer--biweekly IRIS regimen]. (2006)

We planned to conduct a phase II clinical study of combination therapy with CPT-11 and S-1. The antitumor effect was the primary endpoint, while the safety, progression-free survival time, and median survival time were the secondary endpoints. The subjects were untreated patients with inoperable advanced colorectal cancer aged 20-75 years. Based on the results of our previous phase I/II study in patients with gastric cancer, the dosage was established in consideration of safety for outpatient therapy. CPT-11 was administered at a dose of 100 mg/m2 (on days 1 and 15) as an intravenous infusion over 90 minutes, and oral S-1 (40 mg/m2) was administered after breakfast and dinner and then withdrawn for 2 weeks. No other serious adverse reactions occurred (either hematological or non-hematological), and all patients could receive therapy safely on an outpatient basis. Interim analysis suggested excellent results, with a response rate of 50%. Combination therapy with CPT-11 and S-1 achieved a high response rate and could be given safely. These findings suggest that the therapy has potential as first-line treatment for inoperable advanced recurrent colorectal cancer.
Spin-Transfer Torques Generated by the Anomalous Hall Effect and Anisotropic Magnetoresistance (2015)

Spin-orbit coupling is a well-studied means to change the orientation of a spin, and thus flip a logic bit in a spintronic device. The authors offer an advance by showing how the anomalous Hall effect and the anisotropic magnetoresistance, both discovered in the 19th century, can be used like the spin Hall effect to generate spin-orbit torques. This approach provides greater control over the angular dependence of torque than existing methods, and can switch perpendicularly magnetized samples, or move domain walls efficiently.
A low-cost quantitative continuous measurement of movements in the extremities of people with Parkinson's disease (2019)

The assessment of Parkinson's disease currently relies on the history of the present illness, the clinical interview, the physical examination, and structured instruments. Drawbacks to the use of clinical ratings include the reliance on real-time human vision to quantify small differences in motion and significant inter-rater variability due to inherent subjectivity in scoring the procedures. Rating tools are semi-quantitative by design, however, in addition to significant inter-rater variability, there is inherent subjectivity in administering these tools, which are not blinded in clinical settings. Sophisticated systems to quantify movements are too costly to be used by some providers with limited resources. A simple procedure is described to obtain continuous quantitative measurements of movements of people with Parkinson's disease for objective analysis and correlation with visual observation of the movements. •Inexpensive accelerometers are attached to the upper and lower extremities of patients with Parkinson's disease and related conditions to generate a continuous, three-dimensional recorded representation of movements occurring while performing tasks to characterize the deficits of Parkinson's disease.•Movements of the procedure are rated by trained examiners live in real-time and later by videotapes.•The output of the instrumentation can be conveyed to experts for interpretation for diagnostic and therapeutic purposes.
De Novo Human Leukocyte Antigen Allosensitization in Heartmate 3 Versus Heartmate II Left Ventricular Assist Device Recipients (2021)

Left ventricular assist devices (LVADs) are associated with the development of antihuman leukocyte antigen (HLA) antibodies, which can create a challenge for future transplantation in these patients. The differential effects of Heartmate 3 (HM3) versus Heartmate II (HMII) on de novo HLA allosensitization remain unknown. Patients who underwent HMII or HM3 implantation and had no prior HLA antibodies by solid-phase assay (Luminex) testing were included in this study. Complement-dependent cytotoxicity (CDC) panel reactive antibody (PRA) levels and Luminex antibody profiles were followed until cardiac transplantation, device explantation, or death. Electronic medical records were reviewed to examine posttransplant outcomes. Thirty-eight HM3 and 34 HMII patients with complete data were followed for 1.5 ± 1.1 years on device support. HM3 and HMII groups had similar age at implant, female gender, ischemic heart failure etiology, bridge strategy at implant, as well as intraoperative and postoperative transfusion requirements. 39.5% of HM3 and 47.1% of HMII patients developed detectable HLA antibodies by Luminex testing (p = 0.516). Development of high-level (mean fluorescence intensity >10,000) antibodies was significantly lower in HM3 than HMII patients (5.3 vs. 20.6%, p = 0.049). CDC PRA testing showed fewer HM3 patients with a positive result (PRA > 0%) than HMII patients (39.4 vs. 70.0%, p = 0.015). Among transplanted patients, those who had developed de novo sensitization on LVAD support showed a trend toward incidence of moderate to severe grade rejection compared with unsensitized patients (23.8 vs. 4.8%, p = 0.078). HM3 is associated with lower risk of de novo HLA sensitization compared with HMII.
Multiple Dose Study Evaluating the Analgesic Efficacy of Ibuprofen Plus Caffeine Compared to Ibuprofen and Placed in Women with Primary Dysmenorrhea (1996)

Clinical Pharmacology & Therapeutics (1996) 59, 131–131; doi: 10.1038/sj.clpt.1996.23
Increase of high-frequency (&lt;150 hz) intracranial EEG activity during face perception in humans (2003)

We have analyzed high-frequency components of the intracranial EEG activity in response to visual stimuli in the occipital and temporal cortex of epileptic patients. Face perception in particular elicited modulations in the gamma band at frequency ranges up to 250 Hz. This study suggests that the response to complex visual stimuli extends well beyond the common frequency components usually studied in evoked potentials.