gebhart/patrepeval huggingface dataset:
SLC6A14, an amino acid transporter, modifies the primary CF defect in fluid secretion (2018)

The severity of intestinal disease associated with Cystic Fibrosis (CF) is variable in the patient population and this variability is partially conferred by the influence of modifier genes. Genome-wide association studies have identified <i>SLC6A14,</i> an electrogenic amino acid transporter, as a genetic modifier of CF-associated meconium ileus. The purpose of the current work was to determine the biological role of <i>Slc6a14,</i> by disrupting its expression in CF mice bearing the major mutation, F508del. We found that disruption of <i>Slc6a14</i> worsened the intestinal fluid secretion defect, characteristic of these mice. In vitro studies of mouse intestinal organoids revealed that exacerbation of the primary defect was associated with reduced arginine uptake across the apical membrane, with aberrant nitric oxide and cyclic GMP-mediated regulation of the major CF-causing mutant protein. Together, these studies highlight the role of this apical transporter in modifying cellular nitric oxide levels, residual function of the major CF mutant and potentially, its promise as a therapeutic target.
Personalized Pricing and Quality Differentiation on the Internet (2002)

No changes were made in the Abstract. Please use the previous Abstract that was submitted
Rearrangement of Propargylic Esters: Metal‐Based Stereospecific Synthesis of (E)‐ and (Z)‐Knoevenagel Derivatives. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
The calculation and distribution of the turbo-generator iron loss and its influence on the temperature field (2008)

By using numerical calculation method, the electromagnetic field of a turbine generator was calculated and the iron loss in stator yoke and tooth was analyzed respectively, and the distribution of iron loss in stator core could be obtained. Compared with the iron loss calculated from traditional method, the numerical calculated result is close to the measured value. Then taking the iron loss as the heat source, temperature field of a tooth and a slot was calculated. Comparing the obtained results with test data, the temperature field of distribution heat source is closer to the experimental value. Thus some reasonable conclusions were obtained, which can provide some help for the design of turbine generator.
Enabling location-based services on wireless LANs (2004)

The wide deployment of IEEE 802.11 Wireless Local Area Networks (WLANs) has made possible the development of application services on WLANs. Due to the small cell size in WLANs, it is practical to develop location dependent services based upon the awareness of the WLAN access points (AP) that mobile devices currently access. In this paper, we propose a location determination technique using the inherent SNMP support in WLAN APs. It is shown that the location of a mobile device can be determined effectively from the SNMP traps sent by APs. Our SNMP-based approach doesn’t need any particular software or hardware in mobile devices. This paper also considers how to determine the location of a mobile device by its IP address. In general, only the IP address of a mobile device can be seen by location-based services. However, APs identify each mobile device by its MAC address. Hence, we propose effective techniques for the mappings between IP addresses and MAC addresses. Through the address mappings, the location of a mobile device can be determined by its current IP address. We further propose a web service framework to enable WLAN location-based services in WWW environments. To verify our approach, we also develop a location-based campus map service based on the web service framework.
Persistency of Prediction Accuracy and Genetic Gain in Synthetic Populations Under Recurrent Genomic Selection (2017)

Recurrent selection (RS) has been used in plant breeding to successively improve synthetic and other multiparental populations. Synthetics are generated from a limited number of parents [Formula: see text] but little is known about how [Formula: see text] affects genomic selection (GS) in RS, especially the persistency of prediction accuracy ([Formula: see text]) and genetic gain. Synthetics were simulated by intermating [Formula: see text]= 2-32 parent lines from an ancestral population with short- or long-range linkage disequilibrium ([Formula: see text]) and subjected to multiple cycles of GS. We determined [Formula: see text] and genetic gain across 30 cycles for different training set (<i>TS</i>) sizes, marker densities, and generations of recombination before model training. Contributions to [Formula: see text] and genetic gain from pedigree relationships, as well as from cosegregation and [Formula: see text] between QTL and markers, were analyzed via four scenarios differing in (i) the relatedness between <i>TS</i> and selection candidates and (ii) whether selection was based on markers or pedigree records. Persistency of [Formula: see text] was high for small [Formula: see text] where predominantly cosegregation contributed to [Formula: see text], but also for large [Formula: see text] where [Formula: see text] replaced cosegregation as the dominant information source. Together with increasing genetic variance, this compensation resulted in relatively constant long- and short-term genetic gain for increasing [Formula: see text] > 4, given long-range LD<sub>A</sub> in the ancestral population. Although our scenarios suggest that information from pedigree relationships contributed to [Formula: see text] for only very few generations in GS, we expect a longer contribution than in pedigree BLUP, because capturing Mendelian sampling by markers reduces selective pressure on pedigree relationships. Larger <i>TS</i> size ([Formula: see text]) and higher marker density improved persistency of [Formula: see text] and hence genetic gain, but additional recombinations could not increase genetic gain.
The measurement of KNN, KLL in pd→nX and p9Be→nX at 800 MeV (1981)

The spin transfer parameters, KNN and KLL have been measured in pd→nX and p9Be→nX at 0° and 800 MeV. The rather large values of KLL demonstrate that this transfer mechanism will provide a useful source of polarized neutrons at LAMPF energies.
The presence of masked antiribosomal P autoantibodies in healthy children (1998)

These results show that anti-P autoantibodies are present in all healthy children. The majority of these autoantibodies are masked by IgG antibodies, suggesting concordant development of a regulatory network.
NUCLEAR LOCALIZATION OF ACTIVE MATRIX METALLOPROTEINASE-2 IN CIGARETTE SMOKE-EXPOSED APOPTOTIC ENDOTHELIAL CELLS (2009)

Cigarette smoke (CS)-induced activation of proteases such as matrix metalloproteinases (MMPs) contributes to lung alveolar destruction due to cell death. The aim of this study was to determine whether MMPs are produced in pulmonary artery endothelial cells (PAECs) and whether CS activation of MMPs is associated with apoptosis. Cultured PAECs were exposed to CS and subjected to assessments of apoptosis and MMPs. Western blotting and in situ zymography were performed to localize gelatinolytic activity and to identify enzymes. CS-induced apoptosis, i.e., enhanced annexin V binding and cleaved poly-ADP-ribose-polymerase (PARP), correlated with increased degradation of gelatin, a substrate of MMPs. The levels of pro-MMP-2 and active MMP-2 were increased in cytosolic and nuclear fractions isolated from CS-exposed cells. MMP-2 protein colocalized with gelatinolytic activity in the nucleus of CS-exposed cells undergoing apoptosis. These observations support the notion that MMP-2 contributes to CS-induced gelatinase activity, which localizes in the nuclear region primarily and correlates with annexin V binding and PARP cleavage. This suggests a novel function of MMP-2 in the degradation of the nuclear matrix in CS-induced endothelial apoptosis.
Defective vascular development in connexin 45-deficient mice (2000)

In order to reveal the biological function(s) of the gap-junction protein connexin 45 (Cx45), we generated Cx45-deficient mice with targeted replacement of the Cx45-coding region with the lacZ reporter gene. Heterozygous Cx45(+/)(-) mice showed strong expression of the reporter gene in vascular and visceral smooth muscle cells. Cx45-deficient embryos exhibited striking abnormalities in vascular development and died between embryonic day (E) 9.5 and 10.5. Differentiation and positioning of endothelial cells appeared to be normal, but subsequent development of blood vessels revealed impaired formation of vascular trees in the yolk sac, impaired allantoic mesenchymal ingrowth and capillary formation in the labyrinthine part of the placenta, and arrest of arterial growth, including a failure to develop a smooth muscle layer surrounding the major arteries of the embryo proper. As a consequence, the hearts of most Cx45-deficient embryos were dilated. The abnormal development of the vasculature in the yolk sac of Cx45(-)(/)(-) embryos could be caused by defective TGFbeta signalling, as the amount of TGF beta1 protein in the epithelial layer of the yolk sac was largely decreased in the E9.5 Cx45(-)(/)(-) embryo, compared with the wild-type embryo. The defective vascular development was accompanied by massive apoptosis, which began in some embryos at E8.5 and was abundant in virtually all tissues of the embryos at E9.5. We conclude that in Cx45(-)(/)(-) embryos, vasculogenesis was normal, but subsequent transformation into mature vessels was interrupted. Development of different types of vessels was impaired to a varying extent, which possibly reflects the complementation by other connexin(s).
Comparison of Dissolution Behavior of Vancomycin Hydrochloride Preparations for Injection (2010)

The purpose of this study was to examine differences in dissolution behavior between the original vancomycin hydrochloride injection preparation and 3 generics of it.The 4 injection preparations were dissolved in water for injection as described in the package inserts,or in normal saline,and their dissolution behavior was investigated after 15 seconds and 30 seconds.In addition,a kit formulation (Otsuka normal saline 2-Port) was used to investigate dissolution behavior when dissolution was performed either 2 or 3 times.There were differences in dissolution rate between the 2 dissolution media with more than 90% of all the products dissolving within 15 seconds in water for injection and within 30 seconds in normal saline.In the case of performing dissolution twice with the kit formulation,the dissolution rate for all products was also more than 90%.In conclusion,at least 15 seconds or 30 seconds,respectively,is required to dissolve more than 90% of the products in water for injection and normal saline.Also to dissolve more than 90% of the products in a kit formulation,dissolution must be performed at least twice.
A relation based measure of semantic similarity for Gene Ontology annotations (2008)

We derive a measure of semantic similarity between annotations that exploits all available information without introducing assumptions about the nature of the ontology or data. We preserve the principles underlying instance based methods of semantic similarity of terms at the annotation level. As a result our measure better describes the information contained in annotations associated with gene products and as a result is better suited to characterizing and classifying gene products through their annotations.
0610 The effects of wakefulness promoting agents on neuronal activity in nucleus accumbens (2023)

Abstract Introduction Both modafinil and methylphenidate are frequently utilized for maintenance of wakefulness in narcolepsy and other sleep disorders. Both have been demonstrated to affect dopamine transporter, leading to an increase extracellular dopamine. Subsequent functional connectivity changes between nucleus accumbens and pallidum are noted. Animal studies demonstrate changes in neuronal activity with methylphenidate in nucleus accumbens. To our knowledge there has been no direct examination of human neural activity in nucleus accumbens after administration of modafinil or methylphenidate, compared to baseline. Methods Two subjects with placement of stereotactic electroencephalographic (sEEG) electrodes to identify optimal location for permanent deep brain stimulation electrodes in nucleus accumbens were given wakefulness promoting agents to optimize testing of stimulation sites during inpatient hospitalization. Assessment occurred at least 48 hours following surgical electrode insertion under inhaled general anesthesia. Retrospective analysis was performed on neural activity in nucleus accumbens during rest on and off medication. Frequency content was calculated in the following bands: delta (1-3 Hz), theta (4-7 Hz), alpha (8-12 Hz), beta (13-30 Hz), gamma (30-50 Hz), and high gamma (70-100 Hz). Power in each band is reported as the percentage contribution of each band to summed power across all bands. Results Total power was noted to be lower with both modafinil and methylphenidate. With modafinil, the beta band ratio was decreased (22.3% vs. 30.5%), the gamma band ratio was increased (24% vs. 22.4%), and the high gamma band was increased (39.5% vs. 32.9%). With methylphenidate, only the high gamma band was increased (40.3% vs. 22.9%). Conclusion Both methylphenidate and modafinil increase high gamma band neural activity in nucleus accumbens, while modafinil has additional effects on beta and gamma bands. Further assessment of modafinil and methylphenidate’s effects on neural activity are indicated to help better understand their mechanism of action and appropriate use as wakefulness promoting agents. Support (if any)
Blockchain-Based Healthcare Workflow for Tele-Medical Laboratory in Federated Hospital IoT Clouds (2020)

In a pandemic situation such as that we are living at the time of writing of this paper due to the Covid-19 virus, the need of tele-healthcare service becomes dramatically fundamental to reduce the movement of patients, thence reducing the risk of infection. Leveraging the recent Cloud computing and Internet of Things (IoT) technologies, this paper aims at proposing a tele-medical laboratory service where clinical exams are performed on patients directly in a hospital by technicians through IoT medical devices and results are automatically sent via the hospital Cloud to doctors of federated hospitals for validation and/or consultation. In particular, we discuss a distributed scenario where nurses, technicians and medical doctors belonging to different hospitals cooperate through their federated hospital Clouds to form a virtual health team able to carry out a healthcare workflow in secure fashion leveraging the intrinsic security features of the Blockchain technology. In particular, both public and hybrid Blockchain scenarios are discussed and assessed using the Ethereum platform.
Projecting the Minimum Size of HIV Infection and AIDS Epidemic in China (1992)

At present, intravenous drug users (IVDUs) comprise the largest risk group to develop acquired immunodefeciency syndrome (AIDS) in China, and their sex partners seem the second largest risk group. It is necessary to understand long-term trends in the spread of human immunodeficience virus (HIV) among IVDUs and their sex partners and the trends about AIDS cases development for disease prevention and control. The compartment model was used to project the number of HIV infectious individuals and the number of AIDS patients for the next 10 years. The model assumes that transmission is concentrated among IVDUs and their sex partners according to the current situation. The model estimes that by the year 2002, the number of HIV positives will reach 2, 891, AIDS cumulative cases to 1, 124, out of which 776 will have died of the disease ; if we include oral drug users who also have experienced intravenous drug use, HIV positives will be 3, 304, AIDS cases 1, 285, death 887; if unsurveyed IVDUs are included, HIV positives can be estimated to be 4, 129, AIDS cases 1, 606, death 1, 109; with increasing knowledge about AIDS, if persons at risk stop their risk behaviour are doubled, HIV positives will be 2, 496, AIDS cases 1, 024, death 714. these conclusions indicate that prevention efforts among male IVDUs and their female sex partners must be appropriately directed.
ChemInform Abstract: Regio‐ and Chemoselective Alkylation of L‐Ascorbic Acid under Mitsunobu Conditions. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
3D printing of cordierite materials from raw reactive mixtures (2022)

[EN] Porous cordierite materials are 3D printed by robocasting from two kaolin containing raw materials mixtures. Water suspensions of both mixtures at variable solid concentrations (40–67 wt%) are characterized by rheological measurements, showing good printability for concentrations &gt;60 wt% without the need of any printing additive. The mixtures react during sintering (at 1250 °C) giving indialite as the main phase in the structures, which differ in minor phases. Three types of lattices are printed for both compositions with a logpile inner structure. Properties of interest like the coefficient of thermal expansion (CTE), the thermal conductivity (K) and the compression strength (σ) of the printed cordierites are determined and compared with published data. Results evidence that printing of clay containing reactive mixtures is a straightforward and cost-effective way to achieve porous complex shaped cordierite with CTE∼ 2–3 x10 K, K ∼ 0.4–0.6 W m K and maximum σ of 24 MPa.
Intraluminal Middle Cerebral Artery Occlusion Model in Mice (2008)

Intraluminal middle cerebral artery occlusion (MCAO) model in rodents has been widely used for cerebral ischemia studies. The procedure is relatively simple and noninvasive compared to the other focal cerebral ischemia models, and the ischemic insult is highly reproducible. The use of genetically modified mice has been attracting attention because it allows dissection of molecular and cellular mechanisms of various pathological conditions in the brain, and therefore the mouse intraluminal MCAO model has become more prevalent. We describe here our method of mouse intraluminal MCAO and characterize its effects on infarct areas, local cerebral blood flow, extracellular amino acids concentration, and physiological parameters.
Gene Expression Report from HER-2 Signaling, Acquisition of Growth Factor Independence, and Regulation of Biological Networks Associated with Cell Transformation (2023)

Gene Expression Report from HER-2 Signaling, Acquisition of Growth Factor Independence, and Regulation of Biological Networks Associated with Cell Transformation
Correlation and classification of single kernel fluorescence hyperspectral data with aflatoxin concentration in corn kernels inoculated withAspergillus flavusspores (2010)

The objective of this study was to examine the relationship between fluorescence emissions of corn kernels inoculated with Aspergillus flavus and aflatoxin contamination levels within the kernels. Aflatoxin contamination in corn has been a long-standing problem plaguing the grain industry with potentially devastating consequences to corn growers. In this study, aflatoxin-contaminated corn kernels were produced through artificial inoculation of corn ears in the field with toxigenic A. flavus spores. The kernel fluorescence emission data were taken with a fluorescence hyperspectral imaging system when corn kernels were excited with ultraviolet light. Raw fluorescence image data were preprocessed and regions of interest in each image were created for all kernels. The regions of interest were used to extract spectral signatures and statistical information. The aflatoxin contamination level of single corn kernels was then chemically measured using affinity column chromatography. A fluorescence peak shift phenomenon was noted among different groups of kernels with different aflatoxin contamination levels. The fluorescence peak shift was found to move more toward the longer wavelength in the blue region for the highly contaminated kernels and toward the shorter wavelengths for the clean kernels. Highly contaminated kernels were also found to have a lower fluorescence peak magnitude compared with the less contaminated kernels. It was also noted that a general negative correlation exists between measured aflatoxin and the fluorescence image bands in the blue and green regions. The correlation coefficients of determination, r(2), was 0.72 for the multiple linear regression model. The multivariate analysis of variance found that the fluorescence means of four aflatoxin groups, <1, 1-20, 20-100, and >or=100 ng g(-1) (parts per billion), were significantly different from each other at the 0.01 level of alpha. Classification accuracy under a two-class schema ranged from 0.84 to 0.91 when a threshold of either 20 or 100 ng g(-1) was used. Overall, the results indicate that fluorescence hyperspectral imaging may be applicable in estimating aflatoxin content in individual corn kernels.
Cryptic-Plasmid-Free Gonococci May Contribute to Failure of cppB Gene-Based Assays To Confirm Results of BD ProbeTEC PCR for Identification of Neisseria gonorrhoeae (2005)

We note the recent article by Koenig et al. (3) which describes discordance between results obtained with the BD ProbeTEC ET System (BDPT) and a cppB gene-based PCR assay for the detection of Neisseria gonorrhoeae. Overall, 22.6% of BDPT-positive assays were not confirmed by the cppB gene-based PCR, but agreement was particularly low in those samples producing a reduced method-other-than-acceleration score with the BDPT PCR. The BDPT PCR has been found to cross-react with commensal Neisseria species (5), but such cross-reactions may not adequately explain the high level of nonconfirmed BDPT PCR-positive results obtained in this study. Even with samples with method-other-than-acceleration scores in higher ranges (&gt;20,000), between 6 and 15.5% of positive BDPT PCR results could not be confirmed with the cppB gene-based assay. The authors discussed, among a number of potential reasons for the discrepancy, the possibility that the presence of gonococci lacking the cryptic plasmid where the cppB gene is located may account for this difference. This possibility was disregarded on the basis of studies reporting that 96% of gonococci contained a cryptic plasmid and that even where this plasmid was absent, amplification of a cppB gene sequence replicated in the chromosome may produce the required positive result.