gebhart/patrepeval huggingface dataset:
Structure and Expression of the Murine Slow/Cardiac Troponin C Gene (1989)

Cardiac troponin C (cTnC) is the calcium-binding subunit of the myofibrillar thin filament that regulates excitation-contraction coupling in cardiac muscle. We have utilized a novel polymerase chain reaction cloning procedure to isolate cDNA clones encoding murine cTnC. Murine cTnC is a 161-amino acid polypeptide that has been highly conserved during evolution. Southern blot analyses demonstrated that the cTnC gene is a member of a multigene family. Northern blot analyses revealed that the cTnC gene is expressed in murine cardiac tissue and slow skeletal muscle (soleus), but is not expressed in fast skeletal muscle (extensor digitorum longus and anterior tibialis) or in neonatal or adult brain, kidney, lung, liver, or testis. In addition, while the cTnC gene is not expressed in murine C2C12 myoblasts, differentiation of these cells into myotubes was shown to result in a dramatic induction of cTnC gene expression. A full length cTnC genomic clone was isolated from a murine genomic library by hybridization with a cTnC cDNA probe and structurally characterized by DNA sequence, primer extension, and S1 nuclease protection analyses. The cTnC gene is 3.4 kilobase pairs long and is composed of six exons. The introns do not appear to divide the gene into functional domains. Analysis of the 5'-flanking region of the gene revealed the presence of a consensus TATA box 24 base pairs 5' of the transcription start site. Despite the finding that the gene is expressed only in cardiac and slow skeletal muscle, it lacks the previously described CArG and M-CAT transcriptional regulatory sequence motifs that are involved in regulating the expression of a number of other myofibrillar genes.
Nicotine‐Stimulated Release of [3H]Norepinephrine from Fetal Rat Locus Coeruleus Cells in Culture (1998)

Acute nicotine administration stimulated [3H]norepinephrine ([3H]NE) release from cultured fetal locus coeruleus (LC) cells. The effect was concentration dependent, with an EC50 of 0.9 microM, and was abolished by removal of calcium from, or addition of tetrodotoxin (500 nM) to, the assay buffer. Other nicotinic receptor agonists stimulated [3H]NE release, with the rank order of potency being (+)-epibatidine > (-)-nicotine > 1,1-dimethyl-4-phenylpiperazinium (DMPP). Whereas (-)-nicotine and (+/-)-epibatidine exhibited equal maximal responses, DMPP was a partial agonist and (-)-cytisine had no agonist activity. Nicotine-stimulated release of [3H]NE was blocked by nicotinic receptor antagonists, with an order of potency of mecamylamine > lobeline > cytisine > methyllycaconitine > dihydro-beta-erythroidine. The pharmacological profile of this nicotinic receptor is largely consistent with that described previously for an alpha4beta2 subunit combination, although discrepancies in the efficacies of agonists were observed. No additivity in NMDA- and nicotine-stimulated [3H]NE release was observed, suggesting a common signal transduction mechanism. However, the pharmacological characteristics of MK-801 blockade of nicotine-induced responses were not consistent with those of an NMDA receptor. We therefore conclude that nicotine directly releases [3H]NE from LC cells and does not act indirectly via activation of glutamate release.
SQNL: A New Computationally Efficient Activation Function (2018)

A new activation function is proposed. This activation function uses the square operator to introduce the required non-linearity as compared with the use of an exponential term in the popular TanSig. Smaller computational operation count characterizes the proposed activation function. The key to the effectiveness of this function is a faster convergence when used in Multilayer Perceptron Artificial Neural Network architectural problems. Besides, the derivative of the function is linear, resulting in a quicker gradient computation. The effectiveness and efficiency of the proposed activation function have been compared with the TanSig and the computationally efficient ElliotSig functions using selected UCI datasets. The MNIST handwritten dataset was also used to show the ability of this function on more massive datasets. An empirical comparison suggests that the proposed function outperforms TanSig and ElliotSig in convergence time as well as in generalization metrics for most datasets.
Flavopiridol, A Novel Cyclin-Dependent Kinase Inhibitor, in Metastatic Renal Cancer: A University of Chicago Phase II Consortium Study (2000)

Flavopiridol, at the dose and schedule administered in this trial, is ineffective in metastatic renal cancer. In addition to the diarrhea observed in phase I studies, we also observed a higher incidence of asthenia and serious vascular thrombotic events than expected.
Tumor protein 53‐induced nuclear protein 1 deficiency alters mouse gastrocnemius muscle function and bioenergetics in vivo (2019)

Tumor protein 53-induced nuclear protein 1 (TP53INP1) deficiency leads to oxidative stress-associated obesity and insulin resistance. Although skeletal muscle has a predominant role in the development of metabolic syndrome, the bioenergetics and functional consequences of TP53INP1 deficiency upon this tissue remain undocumented. To clarify this issue, gastrocnemius muscle mechanical performance, energy metabolism, and anatomy were investigated in TP53INP1-deficient and wild-type mice using a multidisciplinary approach implementing noninvasive multimodal-NMR techniques. TP53INP1 deficiency increased body adiposity but did not affect cytosolic oxidative stress, lipid content, and mitochondrial pool and capacity in myocyte. During a fatiguing bout of exercise, the in vivo oxidative ATP synthesis capacity was dramatically reduced in TP53INP1-deficient mice despite ADP level (the main in vivo stimulator of mitochondrial respiration) did not differ between both genotypes. Moreover, TP53INP1 deficiency did not alter fatigue resistance but paradoxically increased the contractile force, whereas there were no differences for muscle fiber-type distribution and calcium homeostasis between both genotypes. In addition, muscle proton efflux was decreased in TP53INP1-deficient mice, thereby indicating a reduced blood supply. In conclusion, TP53INP1 plays a role in muscle function and bioenergetics through oxidative capacity impairment possibly as the consequence of abnormal mitochondrial respiration regulation and/or defective blood supply.
Preliminary Evaluation of Turbulence Level Influence in Heat Transfer Measurements (2004)

Heat transfer coefficients have often been experimentally measured, taking into account Nusselt number as a function of Reynolds and Prandtl number. Most experimenters spend their effort to control turbulence level, set it to different values, or keep it unchanged during the tests, as it’s not easy to predict how its initial level may change final results. The aim of this work is to add some comprehension on how different turbulence incoming levels may affect heat transfer measurements, and when it’s possible or not to neglect such effects. Experimental setup features different duct geometries, and thermocromic liquid crystals coupled with hot-wire anemometers are used as main measurement techniques. Tests were performed for Reynolds number from 10000 to 50000 and turbulence level from 3% to 12%. Several turbulence manipulators were adopted, including aluminum foams and multi-perforated plates, and results show some interesting dependences of heat transfer from both turbulence level and grid features.
Design and Implementation of a flow sampling measurement System on Campus network (2011)

With the rapid development of network technology,Most campus into a Gigabit Network,Campus network increasingly large-scale and complicated,which makes the real-time on-line flow measurement becomes more difficult,Based on sampling method of flow measurement as a scalable measurement technology has become a frequently used and effective flow measurement technology.This paper adopts weakening of the greedy algorithm to determine the segment of the best measuring point,And the use of IP mask matching distributed sampling measurement technology of each measuring point synchronous acquisition flow through sampling after storage to the server in different storage areas,Then according to the measurement of the target on the network acquisition flow gathering,analysis and statistics.From the view of saving cost,this paper introduces two collaborative sampling technique,the double measuring host system acts as a server,measuring apparatus and analyzer role,the entire measurement without a special measuring device and the server,can replace the market special measuring equipment,can achieve the whole campus network flow control.
Elite Controllers Display Higher Activation on Central Memory CD8 T Cells Than HIV Patients Successfully on HAART (2010)

T cell activation plays an important role in driving CD4 depletion during the course of HIV infection. There is scarce information about activation of different T cell subsets in HIV(+) individuals experiencing distinct disease progression. The activation of different CD4(+) and CD8(+) T cell subsets and its contribution to total T cell activation were examined measuring CD38 expression by flow cytometry in 120 HIV-infected individuals and 9 uninfected healthy controls. HIV-infected patients were divided into four groups: 11 elite controllers (EC), 14 viremic controllers (VC), 61 antiretroviral-naive typical progressors (TP), and 34 progressors with viral suppression (VS) under antiretroviral therapy. EC displayed significantly greater activation levels than VS, with a higher contribution of central memory subsets to the activation of total CD8 T cells (p = 0.002). The activation of central memory CD8(+) T cells significantly correlated with viral load in TP regardless of CD4 counts. In contrast with VS, proviral load was undetectable in all EC. Compared to VS, EC display abnormal and higher activation levels of different CD8(+) T cell subsets. Factors other than the size of the viral reservoir should explain the high level of activation of central memory CD8(+) T cells characteristically seen in HIV(+) individuals with spontaneous control of viral replication.
Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages (2020)

Mycobacterial infection leads to activation of the RIG-I/MAVS/TBK1 RNA sensing pathway in macrophages but the consequences of this activation remains poorly defined. In this study, we determined that activation of this RNA sensing pathway stimulates ICAM-1 expression in M.avium-infected macrophage through the inhibition of the E3 ubiquitin ligase CRL4COP1/DET1. CRL4 when active targets the transcription factor ETV5 for degradation by the ubiquitin-proteasome system. In the absence of the ETV5 transcription factor, ICAM-1 expression is significantly decreased. The M.avium-induced ICAM-1 production is required for the formation of immune synapse between infected macrophages and antigen-specific CD4+ T lymphocytes, and is essential for CD4+ T lymphocyte-mediated mycobacterial killing in vitro and in mice. This study demonstrates a previously undefined mechanism by which a host cytosolic RNA sensing pathway contributes to the interplay between mycobacteria infected macrophages and antigen-specific T lymphocytes.
Diamagnetism (2023)

This Glossary contains definitions, explanatory notes, and sources for terms used in physical organic chemistry. Its aim is to provide guidance on the terminology of physical organic chemistry, with a view to achieving a consensus on the meaning and applicability of useful terms and the abandonment of unsatisfactory ones. Owing to the substantial progress in the field, this 2021 revision of the Glossary is much expanded relative to the previous edition, and it includes terms from cognate fields.
Small-chamber 4.7 kJ plasma focus for applications (2001)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation César Moreno, Alejandro Clausse, Horacio Bruzzone, Javier Martı́nez, Roberto Llovera, Aureliano Tartaglione; Small-chamber 4.7 kJ plasma focus for applications. AIP Conf. Proc. 24 April 2001; 563 (1): 276–281. https://doi.org/10.1063/1.1374921 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
An actuator chip for in vitro mechano-stimuli responsive studies of primary cortical cell networks (2016)

To investigate the hypothesis that dynamic nanoscale stimuli can influence central nerve systems functioning, in this paper we develop a chip based on polydimethylsiloxane (PDMS) soft lithography which exerts nanoscale mechanical loads on an in vitro neuronal network by microfluidic pneumatic deformation of a membrane. Initial analysis on Calcium ion (Ca2+) imaging within rat primary cortical networks shows increased Ca2+activities in the neuronal network after a stimuli was introduced, indicating that the neuronal networks respond to the mechanical stimulus.
The Wigner-Ville trispectrum: definition and application (2002)

The Wigner-Ville trispectrum (WVT) is defined as a member of a class of time-varying higher-order spectra based on the polynomial Wigner-Ville distributions. It is shown that the WVT is a very efficient time-frequency analysis method for the analysis of FM signals affected by multiplicative noise, and outperforms the Wigner-Ville spectrum (WVS), and therefore any other method based on Cohen's class of bilinear time-frequency spectra. The authors further consider the instantaneous frequency estimator based on the peak extraction of the WVT. They also develop the statistical properties of such an estimator, for signals affected by both multiplicative and additive Gaussian noise.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
L'hyperaldostéronisme primaire. Mise au point et traitement raisonnés. (1997)

peer reviewed
Water Quality and Organic Pollution with Health Risk Assessment in China: A Short Review (2022)

Although water quality has been improved in the last two decades, water pollution by organic contaminants has remained a non-negligible issue in China. It is worthwhile to assess human exposure risks to waterborne organic contaminants and find the relation between them and carcinogenic risks. This work reviews the spatiotemporal patterns of water quality and typical organic contaminants in drinking water sources (i.e., surface water, groundwater, and tap water) in relation to age-standardized incidences of total cancers in China over 16 years. The improvement of water quality for drinking water sources seemed to produce a positive effect in reducing incidences of polluted water-related diseases and digestive system cancers. Although the total accumulative lifetime cancer risk for residents through water drinking and dermal exposure to typical carcinogenic organic contaminants was lower than the latest incidences of digestive system cancers and total cancers excluding lung cancer published by the Chinese government, their similar spatial patterns indicated that this exposure should be considered as one of potential factors for carcinogenic risks. Finally, further efforts to explore the correlation between human exposure to organic contaminants in water and related health effects on a long-term basis should be helpful for establishing and implementing feasible management strategies to reduce disease burdens.
Process Mapping Strategies to Prevent Subcutaneous Implantable Cardioverter-Defibrillator Infection (2022)

Background: Infection remains a major complication of cardiac implantable electronic devices (CIEDs) and can lead to significant morbidity and mortality. Extrathoracic devices that avoid epicardial or transvenous leads, such as the subcutaneous implantable cardioverter-defibrillator (S-ICD), can reduce the risk of serious infection-related complications, such as bloodstream infection and infective endocarditis. While the 2017 AHA/ACC/HRS guidelines include recommendations for S-ICD use for patients at high risk of infection, currently, there are no clinical trial data that address best practices for the prevention of S-ICD infections. Therefore, an expert panel was convened to develop consensus on these topics. Methods: An expert process mapping methodology was used to achieve consensus on the appropriate steps to minimize or prevent S-ICD infections. Two face-to-face meetings of high-volume S-ICD implanters and an infectious diseases specialist, with expertise on cardiovascular implantable electronic device infections, were conducted to develop consensus on useful strategies pre-, peri-, and post-implant to reduce S-ICD infection risk. Results: Expert panel consensus of recommended steps for patient preparation, S-ICD implantation, and post-operative management were developed to provide guidance in individual patient management. Conclusion: Achieving expert panel consensus by process mapping methodology for S-ICD infection prevention was attainable, and the results should be helpful to clinicians in adopting interventions to minimize risks of S-ICD infection.
Ridge-valley lines on meshes via implicit surface fitting (2004)

We propose a simple and effective method for detecting view-and scale-independent ridge-valley lines defined via first- and second-order curvature derivatives on shapes approximated by dense triangle meshes. A high-quality estimation of high-order surface derivatives is achieved by combining multi-level implicit surface fitting and finite difference approximations. We demonstrate that the ridges and valleys are geometrically and perceptually salient surface features, and, therefore, can be potentially used for shape recognition, coding, and quality evaluation purposes.
Tri-axial graphics as an aid to understanding left ventricular function in conscious dogs (1984)

Ultrasonic distance gauges were implanted in the left ventricle of dogs to measure continuously ventricular wall thickness and small axis diameter. A miniature transducer was implanted to measure intraventricular pressure. The circumflex artery could be occluded mechanically to mimic coronary spasm. Recordings were made on tape after allowing eight days post-operative recuperation. The taped signals were digitised and processed to show graphical relationships between selected variables. We found that triaxial (3D) graphics greatly enhanced our ability to interpret moment by moment changes in these relationships, and recommend this procedure to others.
Spatial resolution comparison of a diffractive plenoptic camera and an intermediate image diffractive plenoptic camera (2019)

The diffractive plenoptic camera (DPC) was developed as a system that would capture the spectral and spatial information of a scene in one snapshot. While the DPC couples a diffractive optic with plenoptic camera designs to provide snapshot spectral imaging capabilities, it produces rendered images with low pixel count and low spatial resolution. A modified setup of the DPC, the intermediate image (II)DPC, was built and tested for the first time and compared to both the DPC and a diffractive-optic camera as a system that could improve the cutoff spatial frequency of the rendered images. This paper reports on the spatial resolution achieved for different configurations of the IIDPC and looks at the factors limiting performance. The IIDPC improved on the cutoff spatial resolution over the DPC over a wavelength range of 750 to 790 nm for a design wavelength of 770 nm and improved resolution over a diffractive-optic camera at wavelengths below 750 nm or above 790 nm, with the best results achieved for IIDPC configurations with the largest magnification. Frequency analysis of each system determined that the optic limiting performance was the microlens array. Models showed that decreasing the microlens size improved resolution but reduced the spectral range for the DPC, while decreasing the <italic>f</italic> / <italic>#</italic> of the microlenses improved the resolution for the IIDPC. These results will help optimize the designs of future systems.
Analyzing Gene Relationships for Down Syndrome with Labeled Transition Graphs (2007)

The relationship between changes in gene expression and physical characteristics associated with Down syndrome is not well understood. Chromosome 21 genes interact with nonchromosome 21 genes to produce Down syndrome characteristics. This indirect influence, however, is difficult to empirically define due to the number, size, and complexity of the involved gene regulatory networks. This work links chromosome 21 genes to non-chromosome 21 genes known to interact in a Down syndrome phenotype through a reachability analysis of labeled transition graphs extracted from published gene regulatory network databases. The analysis provides new relations in a recently discovered link between a specific gene and Down syndrome phenotype. This type of formal analysis helps scientists direct empirical studies to unravel chromosome 21 gene interactions with the hope for therapeutic intervention.
D6 facilitates cellular migration and fluid flow to lymph nodes by suppressing lymphatic congestion (2011)

Lymphatic endothelial cells are important for efficient flow of antigen-bearing fluid and antigen-presenting cells (APCs) from peripheral sites to lymph nodes (LNs). APC movement to LNs is dependent on the constitutive chemokine receptor CCR7, although how conflicting inflammatory and constitutive chemokine cues are integrated at lymphatic surfaces during this process is not understood. Here we reveal a previously unrecognized aspect of the regulation of this process. The D6 chemokine-scavenging receptor, which is expressed on lymphatic endothelial cells (LECs), maintains lymphatic surfaces free of inflammatory CC-chemokines and minimizes interaction of inflammatory leukocytes with these surfaces. D6 does not alter the level of CCR7 ligands on LECs, thus ensuring selective presentation of homeostatic chemokines for interaction with CCR7(+) APCs. Accordingly, in D6-deficient mice, inflammatory CC-chemokine adherence to LECs results in inappropriate perilymphatic accumulation of inflammatory leukocytes at peripheral inflamed sites and draining LNs. This results in lymphatic congestion and impaired movement of APCs, and fluid, from inflamed sites to LNs. We propose that D6, by suppressing inflammatory chemokine binding to lymphatic surfaces, and thereby preventing inappropriate inflammatory leukocyte adherence, is a key regulator of lymphatic function and a novel, and indispensable, contributor to the integration of innate and adaptive immune responses.