gebhart/patrepeval huggingface dataset:
Discovery of Solabiose Phosphorylase and its Application for Enzymatic Synthesis of Solabiose from Sucrose and Lactose (2021)

Abstract Glycoside phosphorylases (GPs), which catalyze the reversible phosphorolysis of glycosides, are promising enzymes for the efficient production of glycosides. Various GPs with new catalytic activities, derived from uncharacterized proteins phylogenetically distant from known enzymes, have been discovered in the past decade. In this study, we characterized the PBOR_28850 protein, belonging to glycoside hydrolase family 94, from Paenibacillus borealis . Screening of acceptor substrates for reverse phosphorolysis using α-d-glucose 1-phosphate as the donor substrate revealed that the recombinant PBOR_28850 produced in Escherichia coli specifically utilized d-galactose as an acceptor and produced solabiose (b-d-Glc p -(1→3)-d-Gal). This indicates that PBOR_28850 is a new GP, solabiose phosphorylase. PBOR_28850 catalyzed the phosphorolysis and synthesis of solabiose through a sequential bi-bi mechanism involving the formation of a ternary complex. The production of solabiose from lactose and sucrose has been established. Lactose was hydrolyzed to d-galactose and d-glucose by β-galactosidase. Phosphorolysis of sucrose and synthesis of solabiose were then coupled by adding sucrose, sucrose phosphorylase, and PBOR_28850 to the reaction mixture. Using 210 mmol lactose and 280 mmol sucrose, 207 mmol of solabiose was produced. Yeast treatment degraded the remaining monosaccharides and sucrose without reducing solabiose. Solabiose with a purity of 93.7% was obtained without any chromatographic procedures.
A comprehensive investigation of the condensation of furanic platform molecules to C14–C15 fuel precursors over sulfonic acid functionalized silica supports (2018)

An approach for the utilization of furfural and its hydrogenation products in the synthesis of high carbon fuel precursors over silica supported sulfonic acid catalysts is reported.
Complete long‐term survival data from a trial of adjuvant chemotherapy vs control after radical cystectomy for locally advanced bladder cancer (2005)

The long-term results further support the use of adjuvant-combined chemotherapy with cisplatin-based regimens after radical cystectomy for locally advanced bladder cancer, as this significantly improves progression-free and tumour-specific survival.
Finding shortest non-trivial cycles in directed graphs on surfaces (2015)

Let $D$ be a weighted directed graph cellularly embedded in a surface of genus $g$, orientable or not, possibly with boundary. We describe algorithms to compute shortest non-contractible and shortest surface non-separating cycles in $D$, generalizing previous results that dealt with undirected graphs. Our first algorithm computes such cycles in $O(n^2\log n)$ time, where $n$ is the total number of vertices and edges of $D$, thus matching the complexity of the best general algorithm in the undirected case. It revisits and extends Thomassen's 3-path condition; the technique applies to other families of cycles as well. We also provide more efficient algorithms in special cases, such as graphs with small genus or bounded treewidth, using a divide-and-conquer technique that simplifies the graph while preserving the topological properties of its cycles. Finally, we give an efficient output-sensitive algorithm, whose running time depends on the length of the shortest non-contractible or non-separating cycle.
Content-based Remote Sensing Image Retrieval Based on Ant Colony Optimization and Convolutional Neural Networks (2021)

Content-based Remote sensing image retrieval (CBRSIR) methods usually only use the distance between a query image and retrieved images to retrieve and neglect the semantic similarity among images on the retrieval dataset during the retrieval process. The semantic similarity of these retrieved images helps improve the performance of CBRSIR. Hence, we propose a new retrieval method based on ant colony optimization (ACO) to use the semantic similarity between these retrieved images. The pheromone concentration is used to measure the semantic similarity between these retrieved images for improving retrieval performance. We first present how to initial the pheromone concentration of images. Next, we construct retrieval paths by mimicking the foraging process of ants and use a CNN regression model to accurately evaluate the length of these paths. Then, we update the pheromone concentration of images according to the length of the paths. Finally, we integrate the pheromone of images into the process of CBRSIR for improving retrieval performance. Experiments are conducted on a publicly available remote sensing image (UCMD) dataset. Compared with the state-of-the-art methods, the proposed method can improve retrieval precision.
2016 update Japanese Society for Dialysis Therapy Standard of fluids for hemodialysis and related therapies (2018)

The necessity of purification of dialysis fluids has been discussed since the 1980s. The Japanese Society for Dialysis Therapy (JSDT) presented a standard for dialysis fluid purification in 1995 and revised three times after that. Lastly, JSDT presented a new standard on microbiological management of fluids for Hemodialysis and related therapies in 2008 (the 2008 JSDT standard). It, however, specifies biological contaminants (ET and bacteria) alone. The society further revised the 2008 JSDT standard with the addition of the water quality standard for chemical contaminants and management standard for water treatment equipment in 2016 (the 2016 JSDT standard). This article describes the 2016 JSDT. Chapter 1 describes the water quality standard for biological contaminants without any modification of the standard values in the 2008 JSDT standard because the ultrapure dialysis fluid standard was met at 66.4% of all responder facilities in a 2014 survey conducted by the JSDT. Chapter 2 describes the water quality standard for chemical contaminants. Twelve items are specified in the standard, inasmuch as the toxic potentials of these chemicals have been verified, or are suspected. When the water treatment equipment is operated appropriately, chemical contaminants are appropriately removed from raw water and safe dialysis water is supplied to dialysis machines. However, chemical contaminants in raw water should be confirmed at least before installation of the water treatment equipment. In the daily management, water quality data on the source of raw water must be confirmed in each season of the year. If some chemical contaminants in source of raw water do not meet the water quality standard, the relevant chemical contaminants in dialysis water should be measured at least once a year. Chapter 3 describes the measurement of the residual chlorine. The concentration of total residual chlorine (total chlorine) in dialysis water should be less than 0.1 mg/L. The management standard for water treatment equipment is proposed in Chapter 4. Chapter 5 describes in detail the management.
Ablative Allogeneic Hematopoietic Cell Transplantation in Adults 60 Years of Age and Older (2005)

These data suggest that allogeneic HCT is feasible in selected patients > or = 60 years of age, although novel methods to reduce NRM while maintaining efficacy are needed.
Selenium Regulation of Glutathione Peroxidase in Human Hepatoma Cell Line Hep3B (1993)

Glutathione peroxidase is an important enzyme in cellular antioxidant defense systems, detoxifying peroxides and hydroperoxides. As a component of the glutathione cycle, it protects the liver from reactive oxygen metabolites. Selenocysteine is present at the catalytic site of glutathione peroxidase, and selenium availability regulates glutathione peroxidase enzyme activity. Hep3B cells, a well-differentiated human hepatoma-derived cell line, exhibited time-dependent decrease in glutathione peroxidase activity (nmol NADPH oxidized/min/mg protein, mean +/- SE) when incubated in selenium-free medium for 10 days (Day 0, 21.8 +/- 7.3; Day 2, 10.9 +/- 1.2; Day 4, 7.9 +/- 0.8; Day 6, 4.0 +/- 0.7; Day 8, 4.5 +/- 0.6; Day 10, 1.6 +/- 0.4). With the reintroduction of selenium, glutathione peroxidase activity returned. A second human hepatoma cell line, HepG2, demonstrated a similar pattern when depleted of and then repleted with selenium. To assess protein synthesis, glutathione peroxidase activity was measured in deficient and replete Hep3B cells incubated with and without selenium and with and without cycloheximide. Deficient cells (mean +/- SE) (4.9 +/- 0.2) showed an increase in glutathione peroxidase activity after 24 h in selenium-containing medium (11.6 +/- 0.2), but not when cycloheximide was included in the medium (6.9 +/- 0.5) or when cycloheximide and no selenium was included (5.3 +/- 0.8). Replete Hep3B cells (40.1 +/- 1.1) demonstrated decreased glutathione peroxidase after 24 h in medium without selenium (34.0 +/- 1.4), medium with both cycloheximide and selenium (34.0 +/- 2.6), and medium without selenium and containing cycloheximide (37.6 +/- 1.3). These data suggest that protein synthesis is needed for selenium repletion to exert control on glutathione peroxidase activity. Using a cDNA for human glutathione peroxidase (GPx1), selenium-deficient and replete Hep3B cell RNA was analyzed by Northern blot. mRNA for GPx was quantified by densitometry. The steady-state mRNA level for glutathione peroxidase in deficient cells was 40% of that in replete cells. Nuclear run-on studies to determine the rate of GPx-specific mRNA synthesis showed no difference between nuclei from selenium-replete and selenium-deficient cells. This finding eliminated the possibility of differential transcription rates as an explanation for the observed reduction in mRNA brought about by selenium deficiency and suggested instead a stabilization of mRNA in selenium-replete cells. While selenium deficiency decreased mRNA levels by 60%, glutathione peroxidase enzyme activity decreased by 93%, suggesting a co- and/or post-translational control mechanism in addition to the effect on mRNA stability.
Combined Accelerated Stress Test with In-Situ Thermal Impedance Monitoring to Access LED Reliability (2018)

A combined and accelerated test system (high/low temperature operation life combined with temperature cycle) for In-Situ Transient Thermal Analysis (TTA) was developed. The transient thermal impedance Z <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th(t)</sub> is measured at low and high temperature during temperature cycling and high current operation. The test system is demonstrated using high power LEDs on Al-IMS as devices. By measuring after every cycle the time of occurrence of a failure can be observed more accurate. It is detectable that especially at low temperatures the LED sensitivity factor and thermal load change in the first 100 cyles. Due to the temperature dependency of the thermal capacity of the sapphire (Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> ceramic) the rise of the Z <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th(t)</sub> curves shifts with increased case temperature. The effect is reproduced by transient thermal finite element simulations.
Pre-calibrated Visuo-Haptic Co-location Improves Execution in Virtual Environments (2019)

International audience
Scedosporium apiospermum Soft Tissue Infection As the Initial Presentation of Acute Myeloid Leukemia: A Case Report (2013)

An 82-year-old woman presented with a nonhealing ulcer of her right leg as the first manifestation of acute leukemia. She had bumped her right leg while gardening and developed an ulcer that initially crusted but subsequently evolved a necrotic center. Several weeks later, the patient saw her primary care physician and was treated empirically with cephalexin and then trimethoprim and sulfamethoxazole with no improvement. She was found to have pancytopenia including severe neutropenia. Bone marrow aspirate (Fig 1A ) showed 93% blasts, and a biopsy (Figs 1B [low power] and 1C [high power]) demonstrated a markedly hypercellular marrow composed primarily of immature myeloid cells with a marked decrease in cells of erythroid and megakaryocytic lineages. A diagnosis of acute myeloid leukemia (AML), French-American-British type M0, was made. The marrow karyotype was normal, and a molecular analysis demonstrated wildtype FLT-3 and NPM-1.
Smart antenna technology for structural health monitoring applications (2010)

A smart antenna has been developed for structural health monitoring. The antenna is based on Monarch's GEN 2 selfstructuring antenna (SSA) technology and provides polarization and beam-diversity for improving signal-to-noise ratio (SNR). The antenna works with University of Michigan's Narada platform, where a microcontroller monitors the RSSI and selects the best beam to maintain reliable RF link. Antenna has two wide beams for each polarization and the beams are selected by applying appropriate DC voltages to the RF switches on the antenna aperture. Paper presents the GEN C antenna, which is a smaller version of the GEN 2B with comparable performance features.
Synthesis of Neisseria meningitidis Serogroup W135 Capsular Oligosaccharides for Immunogenicity Comparison and Vaccine Development (2013)

Impfung mit Zuckerle: Kapselsaccharide der N.-meningitidis-Serogruppe W135 wurden synthetisiert. Sera aus Mäusen, die mit diesen Konjugaten aus Di- bis Decasacchariden und Proteinen immunisiert worden waren, wurden mithilfe einer Glykan-Mikroanordnung (siehe Bild) und in einem Bakterizidassay auf Antikörperspezifität und Wirkung gegen Bakterien getestet.
The Synthesis of Ammonium Fluorometallates in Methanol1a (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Synthesis of Ammonium Fluorometallates in Methanol1aHelmut M. Haendler, Frederic A. Johnson, and David S. CrocketCite this: J. Am. Chem. Soc. 1958, 80, 11, 2662–2664Publication Date (Print):June 1, 1958Publication History Published online1 May 2002Published inissue 1 June 1958https://pubs.acs.org/doi/10.1021/ja01544a017https://doi.org/10.1021/ja01544a017research-articleACS PublicationsRequest reuse permissionsArticle Views356Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Physicochemical properties of KF-NaF-AlF3 molten electrolytes (2010)

Conductivity of molten mixtures KF-NaF-AlF3 is measured in the whole concentration range of the [NaF]/([KF] + [NaF]) ratio at the cryolite ratio CR = 1.3 and 1.5 in the temperature range from 800°C to liquidus temperature. Replacement of K+ cations by Na+ ones results in a considerable conductivity increase. Alumina solubility rises with temperature and cryolite ratio ([KF] + [NaF])/[AlF3] in the KF-NaF-AlF3 system, and decreases with sodium fluoride content. Regression equations obtained allow calculating alumina solubility and electrical conductivity in the KF-NaF-AlF3 system in the CR range from 1.3 to 3.0 depending on the concentration of the components and temperature.
Sphingosine 1‐Phosphate Receptor 1 Signaling Maintains Endothelial Cell Barrier Function and Protects Against Immune Complex–Induced Vascular Injury (2018)

Our findings indicate that S1P<sub>1</sub> signaling in ECs modulates vascular responses to IC deposition. S1P<sub>1</sub> agonists and ApoM-Fc enhance the EC barrier, limit leukocyte escape from capillaries, and provide protection against inflammatory injury. The S1P/S1P<sub>1</sub> axis is a newly identified target to attenuate tissue responses to IC deposition and mitigate end-organ damage.
Cough-Induced Syncope as an Unusual Manifestation of Pericardial Effusion (2008)

Introduction Cough-induced syncope belongs to the heterogeneous group of situational syncopes. It consists of the loss of consciousness associated with a paroxysm of cough. It was first described by Charcot in 1876, 1 but the underlying mechanism remained a mystery until recently. While many clinical cases of cough syncope have been described in the literature, this report describes a unique case of cough-induced syncope in a patient with a newlydiscovered pericardial effusion with near tamponade.
One-Pot Formation of Piperidine- and Pyrrolidine-Substituted Pyridinium Salts via Addition of 5-Alkylaminopenta-2,4-dienals to N-Acyliminium Ions: Application to the Synthesis of (±)-Nicotine and Analogs (2010)

Addition of 5-alkylaminopenta-2,4-dienals onto N-acyliminium ions, generated in situ from α-hydroxycarbamates derived from pyrrolidine or piperidine, in the presence of zinc triflate, followed by dehydrative cyclization, allowed the formation of pyridinium salts substituted at their 3-position by a five- or six-membered nitrogen heterocycle. Subsequent N-dealkylation of the pyridinium moiety and deprotection of the secondary amine or reduction of the carbamate function led to (±)-nicotine and analogs.
A paradigm shift for rehabilitation robotics (2008)

Therapeutic robots enhance clinician productivity in facilitating patient recovery. In this article, we presented an overview of the remarkable growth in the activities in the area of therapeutic robotics and of experiences with our devices. We briefly review the published clinical literature in this emerging field and our initial clinical results in stroke. However, we also report our initial efforts that go beyond stroke, broadening the potential population that might benefit from this class of technology by discussing case studies of applications to other neurological diseases. We will also highlight the underexploited potential of this technology as an evaluation tool.
Identification and characterisation ofPmaCl an endonuclease of novel specificity fromPseudomonas maltophila (1986)

We report the use of MonoQ FPLC (Fast Protein Liquid Chromatography) for the rapid purification of a novel Type II restriction endonuclease PmaCI, from Pseudomonas maltophila, which recognises the sequence 5'-CAC decreases GTG-3'. The resulting enzyme is free of other nucleases to a level suitable for its characterisation by multiple-substrate digestion and DNA sequencing techniques. This method appears to be widely applicable and we have used it for the isolation of restriction endonucleases of comparable purity from a range of other organisms. Also described is a rapid method for screening a library of small inserted regions in recombinant M13 molecules for the presence and subsequent screening of restriction sites of interest.
Image segmentation algorithms on female pelvic ultrasound images (2011)

Fragility fractures risk is increased among HIV infected patients. Bone microstructure alterations, in addition to bone mineral density (BMD) reduction, might be responsible for the increased risk. The aim of this study was to determine the prevalence of vertebral fractures (VFs) and their association with trabecular bone score (TBS), an indirect index of bone microstructure, in a cohort of HIV-infected subjects. One-hundred and forty-one HIV-infected patients (87% males, median age 43 years, 94% on stable antiretroviral therapy with undetectable viral load) underwent viro-immunological and bone metabolism biochemical screenings. Lumbar TBS and BMD at femoral neck, total hip, and lumbar spine, were measured with dual-energy X-ray absorptiometry (DXA). VFs were identified using the semiquantitative method and quantitative morphometric analysis from thoracic and lumbar spine X-ray images. VFs were observed in 19 patients (13.5%). BMD was below the expected range for age in 18 (12.8%) subjects. No significant differences were found stratifying VFs prevalence by BMD, whereas patients with lower TBS showed a higher prevalence of VFs (p = 0.03). In multivariate analysis, TBS was the only factor significantly associated to VFs (OR = 0.56; 95% CI = 0.33-0.96; p = 0.034), with increased fracture risk for lower TBS values. VFs are prevalent and associated with low TBS among HIV-positive patients, whereas no significant association was found with BMD.
Novel applications of micro-shock waves in biological sciences (2002)

Spherical micro-shock waves of a few mm radii can be generated both in ambient air and in water by expending a small amount of energy (~1 J). Various techniques such as focusing of laser beam, energy deposition using electrical discharge in water, micro-explosion of silver azide pellets and simultaneous actuation of piezo-ceramic/electrical crystal array can be used for creating micro-shock waves of requisite intensity. The nonlinear instantaneous pressure spike (1 MPa ~100 MPa) generated by the micro-shock waves can be used in applications such as particle delivery systems for gene therapy, food preservation, wood preservation and conditioning, and also in cancer treatment. The visualization studies of micro-shock waves generated using laser beam focusing in ambient air, various types of micro-shock wave generators, the status of current research on various applications along with some experimental results on micro-shock wave-assisted particle delivery systems are discussed in this paper.