gebhart/patrepeval huggingface dataset:
The composition and functionality of bacterial membrane vesicles (bMVs) in Escherichia coli – a time course comparison study in different media (2023)

Abstract Background Bacteria inhabit the in- and outside of the human body, such as skin, gut or the oral cavity where they play an innoxious, beneficial, e.g. as probiotic bacteria, or even pathogenic role, e.g. during a sepsis. Recent studies have shown that bacteria can secrete membrane vesicles (MVs) like eukaryotic cells with extracellular vesicles (EVs). Several studies indicate that bacterial membrane vesicles (bMVs) play a crucial role in microbiome-host interactions. However, the composition of such bMVs and their functionality under different culture conditions are still largely unknown. Methods To get more insights into bMVs, we investigated the composition and functionality of E. coli (DSM 105380) bMVs from the culture media Lysogeny broth (LB) and RPMI 1640 throughout the different phases of growth (lag-, log- and stationary-phase). bMVs from 3 time points from 2 media (LB_8h, LB_54h, LB_168h, RPMI_8h, RPMI_54h and RPMI_168h) were isolated by ultracentrifugation and analyzed using nanoparticle tracking analysis (NTA), EV flow-cytometry, cryogenic electron microscopy (Cryo-EM), conventional transmission electron microscopy (TEM) and mass spectrometry-based proteomics (LC-MS/MS). Furthermore, we examined pro-inflammatory cytokines IL-1 and IL-8 in the human monocyte cell line THP-1 by treatment with bMVs. Results Particle numbers increased with inoculation periods. The bMV morphologies in Cryo-EM/TEM were similar at each time point and condition. EV flow-cytometry showed a similarity of the common bMV markers OmpA + GroEL - and OmpA - GroEL + in each group. We found 140 proteins to be consistently expressed in the 6 groups with LC-MS/MS proteomics while we could also observe unique proteins throughout these treatments. Treatment of THP-1 cells with bMVs of all 6 groups lead to significantly high IL-1 and IL-8 expressions. Conclusions Our study showed that the choice of medium and the duration of culturing significantly influences E.coli bMV protein composition. Moreover, our flow-cytometry results indicate that different bMV subpopulations may be shed. Irrespective of the medium used, we observed an accumulation of E. coli bMVs over time, possibly due to increase of bacterial cells. Our cell culture experiments/functional assays imply that bMVs isolated from the 6 groups by ultracentrifugation retain their function and lead to comparable cytokine induction.
Copolyimides With Flexibilizing Groups (1987)

Copolymers improved flexibility, processability and melt-flow characteristics. Copolyimide of new type exhibits synergistic improvements in flow properties due to flexibilized diamine-derived units incorported into polymer backbone. Technique produces copolyimides with combination of flexible linkage that cause polymers to exhibit flow properties particularly well suited for use in wide range of products including adhesives, molding resins, laminating resins, dielectric and protective coatings. Improved properties make copolymers especially useful as thermoplastic hot-melt adhesives.
Faculty Opinions recommendation of Antisense Oligonucleotide-Mediated Removal of the Polyglutamine Repeat in Spinocerebellar Ataxia Type 3 Mice. (2019)

Spinocerebellar ataxia type 3 (SCA3) is a currently incurable neurodegenerative disorder caused by a CAG triplet expansion in exon 10 of the ATXN3 gene.The resultant expanded polyglutamine stretch in the mutant ataxin-3 protein causes a gain of toxic function, which eventually leads to neurodegeneration.One important function of ataxin-3 is its involvement in the proteasomal protein degradation pathway, and long-term downregulation of the protein may therefore not be desirable.In the current study, we made use of antisense oligonucleotides to mask predicted exonic splicing signals, resulting in exon 10 skipping from ATXN3 pre-mRNA.This led to formation of a truncated ataxin-3 protein lacking the toxic polyglutamine expansion, but retaining its ubiquitin binding and cleavage function.Repeated intracerebroventricular injections of the antisense oligonucleotides in a SCA3 mouse model led to exon skipping and formation of the modified ataxin-3 protein throughout the mouse brain.Exon skipping was long lasting, with the modified protein being detectable for at least 2.5 months after antisense oligonucleotide injection.A reduction in insoluble ataxin-3 and nuclear accumulation was observed following antisense oligonucleotide treatment, indicating a beneficial effect on pathogenicity.Together, these data suggest that exon 10 skipping is a promising therapeutic approach for SCA3.
Coupleur de Lange Multicouche en bande Ka (2009)

National audience
Endothelial Cell Dysfunction—Can One Outsmart Oxidative Stress by Direct Interaction with the Pathological Oxidized or Heme-Free Soluble Guanyl-Cyclase? (2007)

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Eberhard Ritz Feature Editor



It is well known that the rate of CV death is excessive in renal patients ([1][2]), but the underlying causes have not been completely resolved. Endothelial dysfunction, however, is thought to play a central role. One indicator of endothelial
Grid-Tied Solar PV/Fuel Cell Hybrid Power System for University Building (2019)

The results of an optimized design of grid-connected renewable energy system for University building are presented in this paper. Integrated modelling, simulation, optimization and control strategies are used to test the performance and select the best hybrid renewable power system. The main objective is to design a grid connected renewable energy system to meet the electric load of the building with high penetration of renewable energy, low greenhouse gas emissions, and low cost of energy. Hourly simulations, modelling and optimization were performed to determine the performance and cost of the hybrid power system configurations using load following and cycle charging control strategies. The results show that the grid-tied solar PV/fuel cell hybrid power system offers a good performance for the tested system architectures. With a solar PV capacity of 500 kW and fuel cell capacity of 100 kW integrated with the grid, the total energy generated from grid-tied renewable energy system to meet the desired load is 26% from the grid (purchase), 42% produced from the solar PV, and 32% from the fuel cell. From the total annual power produced, 95% is used to meet the AC primary load of the building and 5% is the sellback to the grid. The daily performance and electricity generation from the proposed grid-tied solar PV/Fuel cell power system is also presented in this paper. The proposed grid-tied solar PV/fuel Cell hybrid power system with the sale of electricity back to the grid has a high renewable fraction (40.4 %), low levelized cost of energy (71 $/MWh), and low carbon dioxide emissions (133 kg CO2/MWh).
Lidar detection using a dual-frequency source (2006)

A new technique of dual-frequency Doppler-lidar measurement is investigated. This technique is based on the use of a coherently locked, tunable, dual-frequency laser source and is shown to accurately measure velocities as small as 26 mum/s. It is generated by exploiting the nonlinear dynamics of a semiconductor laser through a proper combination of optical injection and operating conditions.
Design of Low Inductance Busbar for 500 kVA Three-Level ANPC Converter (2019)

The adoption of SiC devices in high power applications enables higher switching speed, which requires lower circuit parasitic inductance to reduce the voltage overshoot. This paper presents the design of a busbar for a 500 kVA three-level active neutral point clamped (ANPC) converter. The layout of the busbar is discussed in detail based on the analysis of the multiple commutation loops, magnetic canceling effect, and DC-link capacitor placement. The loop inductance of the busbar is verified with simulation, impedance measurements, and converter experiments. The results match with each other, and the inductances of small and large loop are 6.5 nH and 17.5 nH respectively, which is significantly lower than the busbars of NPC type converters in other references.
Calcium-dependent inhibitory region of troponin: a proton nuclear magnetic resonance study on the interaction between troponin C and the synthetic peptide N.alpha.-acetyl[FPhe106]TnI-(104-115) amide (1983)

To investigate the calcium-dependent regulation of muscle contraction, a synthetic analogue of the inhibitory region of troponin I, N alpha-acetyl[FPhe106]TnI-(104-115) amide, has been made by solid-phase peptide synthesis. This region represents the minimum sequence necessary for inhibition of actomyosin ATPase activity in the presence of tropomyosin [Talbot, J.A., & Hodges, R.S. (1981) J. Biol. Chem. 256, 2798-2802]. Conformational changes induced by the formation of the synthetic peptide-troponin C complex are followed by proton nuclear magnetic resonance spectroscopy. Aliphatic (Leu and Val), aromatic (p-fluorophenylalanine), and charged (Arg) residues are perturbed by interaction with troponin C. In troponin C, peptide-protein interaction results in the redistribution of the Phe envelope of troponin C and perturbations in the aliphatic region. The observed effects on the protein resonances are in agreement with proposed interaction of the peptide with the N-terminal region of site III of troponin C. In the absence of calcium, this region of troponin I (104-115) is bound to actin-tropomyosin, inhibiting actomyosin ATPase activity [Talbot, J.A., & Hodges, R.S. (1981) J. Biol. Chem. 256, 2798-2802]. Our results suggest that the binding site for this region of troponin I is induced in troponin C in the presence of calcium and the formation of this complex releases actomyosin ATPase inhibition.
Organic metal based on a new unsymmetrical S,Se-containing donor - bis(ethylenedithiodimethyldithiadiselenafulvalene) triiodide (1987)

A peculiarity of organic superconductors of the type (TMTSF)/sub 2/ClO/sub 4/ (TMTSF is tetramethyltetraselenafulvalene) and (BEDT-TTF)/sub 2/I/sub 3/ (BEDT-TTF is bis(ethylenedithio)tetrathiafulvalene) is their substantial two-dimensional character. In the case of BEDT-TTF salts the two-dimensional character is expressed to a greater extent because of the greater length of the ..pi..-electron system due to the additional S atoms introduced into the molecules. In this paper they obtain and investigate salts of an unsymmetrical organic donor that includes fragments of TMTSF and BEDT-TTF molecules and is constructed in such a way that one five-membered ring contains only S atoms, and the other contains only Se atoms. The composition and structure of the metallic phase are established by x-ray diffraction analysis.
Residual Fuel Treating and Handling for Base Load Gas Turbines (1975)

This paper is a discussion of the methods of heavy fuel treatment for industrial gas turbines and the effects of fuel characteristics on washability. A summary of recent experience with burning heavy fuels by a typical industrial user is presented. The paper includes a discussion of the latest design improvements in fuel treating equipment and the resulting improvements in washed fuel quality. The effect of fuel parameters on system size, cost and performance is evaluated.
Tramways of Barcelona: advantages and disadvantages of BOT (Build Operate Transfer) and its influence in concession contracts in Spain (2006)

During the periovulatory period, the profile of fibroblast growth factor 2 (FGF2) coincides with elevated prostaglandin E2 (PGE2) levels. We investigated whether PGE2 can directly stimulate FGF2 production in bovine granulosa cells and, if so, which prostaglandin E2 receptor (PTGER) type and signaling cascades are involved. PGE2 temporally stimulated FGF2. Accordingly, endoperoxide-synthase2-silenced cells, exhibiting low endogenous PGE2 levels, had reduced FGF2. Furthermore, elevation of viable granulosa cell numbers by PGE2 was abolished with FGF2 receptor 1 inhibitor, suggesting that FGF2 mediates this action of PGE2. Epiregulin (EREG), a known PGE2-inducible gene, was studied alongside FGF2. PTGER2 agonist elevated cAMP as well as FGF2 and EREG levels. However, a marked difference between cAMP-induced downstream signaling was observed for FGF2 and EREG. Whereas FGF2 upregulated by PGE2, PTGER2 agonist, or forskolin was unaffected by the protein kinase A (PKA) inhibitor H89, EREG was significantly inhibited. FGF2 was dose-dependently stimulated by the exchange protein directly activated by cAMP (EPAC) activator; a similar induction was observed for EREG. However, forskolin-stimulated FGF2, but not EREG, was inhibited in EPAC1-silenced cells. These findings ascribe a novel autocrine role for PGE2, namely, elevating FGF2 production in granulosa cells. This study also reveals that cAMP-activated EPAC1, rather than PKA, mediates the effect of PGE2/PTGER2 on the expression of FGF2. Stimulation of EREG by PGE2 is also mediated by PTGER2 but, in contrast to FGF2, EREG was found to be PKA sensitive. PGE2-stimulated FGF2 can act to maintain granulosa cell survival; it can also act on ovarian endothelial cells to promote angiogenesis.
Pulse excited interferometric fiber-optic gyroscope (1998)

Summary form only given. We introduce the first pulse-excited interferometric fibre optic gyroscope (IFOG) to our knowledge. The pulse source is advantageous in its ability to produce a wide spectrum and high average power at the same time. It also opens the door to new methods of stabilizing mean wavelength, reducing sidelobes in coherence function, and reducing shot noise via optical squeezing.
A Solid‐Phase Approach to Accessing Bisthioether‐Stapled Peptides Resulting in a Potent Inhibitor of PRC2 Catalytic Activity (2018)

Stapled peptides have emerged as a new class of therapeutics to effectively target intractable protein-protein interactions. Thus, efficient and versatile methods granting easy access to this class of compounds and expanding the scope(s) of the currently available ones are of great interest. Now, a solid phase approach is described for the synthesis of bisthioether stapled peptides with multiple architectures, including single-turn, double-turn, and double-stapled macrocycles. This method allows for ligation with all-hydrocarbon linkers of various lengths, avoiding the use of unnatural amino acids and expensive catalysts, and affords cyclopeptides with remarkable resistance to proteolytic degradation. The potential of this procedure is demonstrated by applying it to generate a stapled peptide that shows potent in vitro inhibition of methyltransferase activity of the polycomb repressive complex 2 (PRC2) of proteins.
Food Science and Food Biotechnology (2003)

This groundbreaking book provides a balanced and organized discussion of the interactions of food science and biotechnology at the molecular and industrial levels. Carefully selected and reviewed contributions stress the aspects of modern bioprocessing, analysis, and quality control that are common to both food science and biotechnology. The detailed analysis of specific examples of upstream and downstream processes assists readers in understanding the underlying principles and the similarities between applications. Throughout the book there is a strong emphasis on emerging technologies, combined methods for preserving food and biological materials, and descriptions of state-of-the-art equipment and techniques.
Discordant synthesis and secretion of human chorionic gonadotropin and subunits by cervical carcinoma cells. (1981)

Epidermoid carcinoma of the cervix cell lines DoT and CaSki were examined for the production of human chorionic gonadotropin (HCG) and its subunits (HCG-alpha and HCG-beta). Additionally, the effect of butyrate and other regulatory agents on synthesis and secretion was investigated. Media and cell lysates were analyzed by radioimmunoassay for HCG and HCG-beta and by radioreceptor assay for intact HCG. Although DoT and CaSki cells secreted more HCG-beta than HCG, the cell lysates contained only one-half as much HCG-beta as HCG. This raises the possibility that HCG-alpha synthesis may be limiting to the formation of intact HCG in DoT and CaSki cell lines. Our results also indicate discordance in HCG and HCG-beta production in response to butyrate. Whereas the production of HCG-beta was increased by 5 mM sodium butyrate, the synthesis and secretion of intact hCG were decreased. These results support the concept of independent regulation of alpha- and beta-subunit synthesis and secretion.
Biological control of plant diseases by inter-cropping and mixed-cropping. (2011)

栃木県に伝わるユウガオの株元に長ネギを混植する伝承技術を応用し，ネギ属植物に親和性のある拮抗細菌（Burkholderia gladioli）を接種し，これを混植することで，ユウガオつる割病（Fusarium oxysporum f.sp. lagenariae），キュウリつる割病（Fusarium oxysporum f.sp. cucumerinum），トマト萎ちょう病（Fusarium oxysporum f.sp. lycopersici），などの土壌病害を防除する方法を開発した。防除効果の発現機構としてはB. gladioliの産生するピロールニトルリン，ネギ属植物が産生するアリシンがFusarium属菌を抗菌することが考えられた。また，ネギ属植物と野菜との混植が土壌微生物相を多様化し，土壌病原菌の活動を抑制したと推察した。また，ウリ類をムギ類や雑草などで草生栽培する伝承技術から，うどんこ病に重寄生する重寄生菌Ampelomyces quisqualisを雑草で増殖させ，雑草を重寄生菌のバンカープランツとして利用し，うどんこ病を防除する方法を検討した。
Resolution Dependent GAN Interpolation for Controllable Image Synthesis Between Domains (2020)

GANs can generate photo-realistic images from the domain of their training data. However, those wanting to use them for creative purposes often want to generate imagery from a truly novel domain, a task which GANs are inherently unable to do. It is also desirable to have a level of control so that there is a degree of artistic direction rather than purely curation of random results. Here we present a method for interpolating between generative models of the StyleGAN architecture in a resolution dependent manner. This allows us to generate images from an entirely novel domain and do this with a degree of control over the nature of the output.
Growth of a Form 2 Mycobacterium and Various Bacillus Species on Lowenstein-Jensen Medium (1964)

SUMMARY: Vegetative organisms of a Form 2 strain of Mycobacterium tuberculosis were inoculated on to Löwenstein-Jensen medium containing malachite green. The multiplication of the organisms was slightly delayed by the presence of the dye, but after a few days growth occurred, even when only a few organisms were used as inoculum. Stimulation of growth of Form 2 mycobacteria by the presence of Form 1 mycobacteria was not observed. Spores of Bacillus subtilis and B. licheniformis grew on Löwenstein-Jensen medium. Organisms other than Bacillus were usually obtained from Löwenstein-Jensen medium slopes deliberately exposed to airborne contamination, but have never been found in experiments in which M. tuberculosis Form 2 organisms were isolated. These results establish the validity of the uninoculated control slopes of Löwenstein-Jensen medium used in all previous experiments in which Form 2 mycobacteria were obtained from Form 1 mycobacteria.
Using semantic contents and WordNet in image retrieval (1997)

article Free Access Share on Using semantic contents and WordNet in image retrieval Authors: Y. Alp Aslandogan Department of EECS, University of Illinois at Chicago Department of EECS, University of Illinois at ChicagoView Profile , Chuck Thier Tribune Media Services Tribune Media ServicesView Profile , Clement T. Yu Department of EECS, University of Illinois at Chicago Department of EECS, University of Illinois at ChicagoView Profile , Jon Zou Department of EECS, University of Illinois at Chicago Department of EECS, University of Illinois at ChicagoView Profile , Naphtali Rishe Florida International University Florida International UniversityView Profile Authors Info & Claims ACM SIGIR ForumVolume 31Issue SIDecember 1997 pp 286–295https://doi.org/10.1145/278459.258591Online:01 July 1997Publication History 60citation1,458DownloadsMetricsTotal Citations60Total Downloads1,458Last 12 Months13Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
PreSERVE-AMI (2016)

URL: http://www.clinicaltrials.gov. Unique identifier: NCT01495364.
Hydrogels as potential probes for investigating the mechanism of lenticular presbyopia (2001)

Hydrogels that exhibit physical and mechanical properties comparable to those of the natural lens were successfully identified, synthesized, and characterized, and the feasibility of endocapsular polymerization was demonstrated.
Loss-less pulse intensity repetition-rate multiplication using optical all-pass filtering (2000)

Schemes based on an optical all-pass filtering techniques for multiplying the intensity repetition-rate of an optical pulse train are examined. These methods are in principle without energy loss and the multiplication factor can be any given integer. Practical implementation using cascaded side-coupled ring resonators or multireflection filters is proposed and analyzed for the first time.
Abstract 3027: Pharmacological regulation of the ABCG2 multidrug transporter in A549 non-small cell lung cancer cells (2019)

Abstract The notoriously drug resistant non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths and the ABCG2 multidrug transporter was shown to be involved in NSCLC drug resistance. Direct drug interactions with this transporter have been extensively studied, while a potential modulation of ABCG2 expression by drugs or hormones is less explored. To study the drug-dependent regulation of ABCG2 expression, we used CRISPR-Cas9 and homologous recombination-mediated DNA repair to generate fluorescent lung cancer reporter cells with preserved endogenous regulatory regions. In A549 lung adenocarcinoma cells, which have two wild type copies of the ABCG2 gene, we replaced the initial coding region of ABCG2 with a green fluorescent protein (eGFP)-coding sequence. In addition, since our previous studies demonstrated that an N-terminally eGFP-tagged ABCG2 protein is properly localized and functional, we have generated A549 cells in which the native ABCG2 coding region was modified to express an eGFP-tagged ABCG2 protein. The eGFP knock-in cells sensitively reported ABCG2 transcriptional regulation, while the cells expressing an eGFP-ABCG2 fusion protein allowed the assessment of the full, functional transporter. Using the engineered A549 cells, we found that several drugs, including anti-cancer medications, glucocorticoids, HDAC inhibitors and hypoxia-mimicking agents, caused a significant induction of ABCG2 expression. In these experiments, an intronic regulatory element was found to play a key role in the specific glucocorticoid induction of ABCG2. We suggest that our transgenic reporter cell lines, amenable to high-throughput studies, can be used to investigate the induction of ABCG2-based drug resistance in cancer and predict potential drug-drug interactions. Understanding how the generation of drug-tolerant persister cells occurs via ABCG2 expression may help the selective elimination of these multidrug resistant cancer cells. Supported by the NKFIH-OTKA grant K115375. Citation Format: Daniella Kovacsics, Nikolett Mészáros, Borbála Tihanyi, Zsolt Matula, Adrienn Borsy, Edit Szabó, György Várady, Tamás Orbán, Ágota Apáti, Ábel Fóthi, Anna Brózik, Balázs Sarkadi. Pharmacological regulation of the ABCG2 multidrug transporter in A549 non-small cell lung cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 3027.