gebhart/patrepeval huggingface dataset:
Effects of substrate stiffness and actomyosin contractility on coupling between force transmission and vinculin–paxillin recruitment at single focal adhesions (2017)

Focal adhesions (FAs) regulate force transfer between the cytoskeleton and ECM-integrin complexes. We previously showed that vinculin regulates force transmission at FAs. Vinculin residence time in FAs correlated with applied force, supporting a mechanosensitive model in which forces stabilize vinculin's active conformation to promote force transfer. In the present study, we examined the relationship between traction force and vinculin-paxillin localization to single FAs in the context of substrate stiffness and actomyosin contractility. We found that vinculin and paxillin FA area did not correlate with traction force magnitudes at single FAs, and this was consistent across different ECM stiffness and cytoskeletal tension states. However, vinculin residence time at FAs varied linearly with applied force for stiff substrates, and this was disrupted on soft substrates and after contractility inhibition. In contrast, paxillin residence time at FAs was independent of local applied force and substrate stiffness. Paxillin recruitment and residence time at FAs, however, were dependent on cytoskeletal contractility on lower substrate stiffness values. Finally, substrate stiffness and cytoskeletal contractility regulated whether vinculin and paxillin turnover dynamics are correlated to each other at single FAs. This analysis sheds new insights on the coupling among force, substrate stiffness, and FA dynamics.
CARACTERIZACIÓN MORFOLÓGICA DE HARINA DE SIETE VARIEDADES DE YUCA Y POLVILLO DE FIQUE POR MICROSCOPÍA ÓPTICA DE ALTA RESOLUCIÓN -MOAR- (2010)

Se evaluaron las características morfológicas de siete variedades de harina nativa de yuca y polvillo de fique. Las muestras fueron dispersadas en albúmina de huevo, posteriormente extendidas sobre placas de vidrio y teñidas con azul de toluidina para su observación al microscopio. Se usó la técnica de microscopía óptica de alta resolución (ñ/IOAR) por contraste diferencial de interferencia (DIC), para observarlas muestras y caracterizar las imágenes. Se encontraron en las harinas granulos de almidón esféricos y semiesféricos algunos con formas truncadas, y estructuras fibrosas de diferentes formas. En el polvillo de fique se observaron microfibrillas lon¬ gitudinales en forma de cintas, celdas y espirales. Este estudio permitió caracterizar la morfología de las materias primas estudiadas, cuya infor¬ mación es punto de partida para la continuidad de su uso en el campo de los materiales biodegradables.
Serum neuroendocrine (NE) markers and clinical characteristics of treatment-emergent small cell neuroendocrine prostate cancer (t-SCNC) in men with metastatic castration resistant prostate cancer (mCRPC): Data from the West Coast Prostate Cancer Dream Team. (2018)

278 Background: Detection of t-SCNC in mCRPC patients relies primarily on histopathologic evaluation (Histo) of a metastatic tumor biopsy (bx), likely leading to underdiagnosis. The clinical features of t-SCNC and the diagnostic utility of serum NE markers (neuron-specific enolase (NSE) and chromogranin (CGA)) were evaluated. Methods: Eligible patients (pts) underwent a metastatic bx at one of 5 centers. Histo was performed by 3 independent pathologists (JH, GT, LT). NE markers were evaluated in a central lab (lower limit = 1 ng/mL). Kruskal-Wallis and chi-square test were used to compare continuous and categorical variables, respectively. Receiver-operative-curve (ROC) analysis of serum NE markers was undertaken. Results: 160 consecutive pts with available Histo and NE markers were included. t-SCNC was found in 27 pts (17%). Detection of t-SCNC was observed in all bx sites, including liver (14%), lymph node (19%) and bone (14%). Clinical features are shown in the Table. By ROC analysis, if both serum NSE was &gt; 6.05 ng/mL and chromogranin was &gt; 3.1 ng/mL, the sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) for the detection of t-SCNC were 95%, 50%, 98%, and 22%, respectively. Conclusions: Many of the classic features of de novo SCNC, including low PSA levels, do not reliably distinguish t-SCNC. In contrast, serum NE markers have diagnostic utility with high sensitivity and NPV, but low specificity and PPV. Heterogeneous NE differentiation may partially account for these findings. Clinical trial information: NCT02432001. [Table: see text]
OTA Evaluation of Mobile Phone Antenna Performance for VoLTE [Measurements Corner] (2018)

This article compares the over-the-air (OTA) performance of tradit ional voice service and voice-over LTE (VoLTE). We present the results of the OTA measurements of the antenna performance of 22 mobile phones commonly used over the last two years. VoLTE is a feature included in LTE that allows voice service to be delivered as data flows. Traditional studies on LTE include only data service and, therefore, OTA for data service, which is measured with the phone held in the browsing position only by the hand, whereas VoLTE uses the same data channel but with the device held next to the head by a hand. Traditional studies on voice service include only OTA in voice service. The figures of merit (FoMs) taken into account for ranking the devices were the total radiated power (TRP) and the total isotropic sensitivity (TIS), which are related to the transmitter and receiver antenna performance, respectively. Our investigation includes results for talk mode using left- and right-hand phantoms next to the head to calculate the maximum power radiated by the transmitter. To evaluate the sensitivity of the receiver, we considered the performance in talk mode using a right-hand phantom next to the head as well as in data mode using only the hand phantom.
Confirmatory Factor Analysis (2012)

This chapter contains sections titled: Introduction Model Specification and Identification Parameter Estimation and Model Assessment Inference for Model Parameters Factor Scores
Instructor Feedback Versus No Instructor Feedback on Performance in a Laparoscopic Virtual Reality Simulator (2013)

NCT01497782.
Evolutionary history of the ancient cutinase family in five filamentous Ascomycetes reveals differential gene duplications and losses and in Magnaporthe grisea shows evidence of sub‐ and neo‐functionalization (2008)

* The cuticle is the first barrier for fungi that parasitize plants systematically or opportunistically. Here, the evolutionary history is reported of the multimembered cutinase families of the plant pathogenic Ascomycetes Magnaporthe grisea, Fusarium graminearum and Botrytis cinerea and the saprotrophic Ascomycetes Aspergillus nidulans and Neurospora crassa. * Molecular taxonomy of all fungal cutinases demonstrates a clear division into two ancient subfamilies. No evidence was found for lateral gene transfer from prokaryotes. The cutinases in the five Ascomycetes show significant copy number variation, they form six clades and their extreme sequence diversity is highlighted by the lack of consensus intron. The average ratio of gene duplication to loss is 2 : 3, with the exception of M. grisea and N. crassa, which exhibit extreme family expansion and contraction, respectively. * Detailed transcript profiling in vivo, categorizes the M. grisea cutinases into four regulatory patterns. Symmetric or asymmetric expression profiles of phylogenetically related cutinase genes suggest subfunctionalization and neofunctionalization, respectively. * The cutinase family-size per fungal species is discussed in relation to genome characteristics and lifestyle. The ancestry of the cutinase gene family, together with the expression divergence of its individual members provides a first insight into the drivers for niche differentiation in fungi.
Palladium nanoparticles loaded on nitrogen and boron dual-doped single-wall carbon nanohorns with high electrocatalytic activity in the oxygen reduction reaction (2018)

Palladium nanoparticles with a diameter of 2-4 nm supported on nitrogen and boron dual-doped single-wall carbon nanohorns (Pd-NBCNHs) are synthesized <i>via</i> a one-step method and their electrocatalytic activities are investigated for the oxygen reduction reaction (ORR) in alkaline media. The electrochemical results demonstrate that the oxygen reduction peak potential of Pd-NBCNHs is similar to that of commercial 20% Pt-C. Furthermore, Pd-NBCNHs show a more positive half-wave potential than 20% Pt-C and display better long-term stability and resistance to methanol than 20% Pt-C, which is attributed to the synergetic effect of the Pd nanoparticles and NBCNHs. As NBCNHs have abundant pyrrolic nitrogen, charged sites and defective structures, they not only act as a carrier, but also provide the active sites for oxygen adsorption during the oxygen reduction reaction process. The outstanding electrochemical performance makes Pd-NBCNHs promising to be applied in fuel cells.
MT1‐MMP: A tethered collagenase (2004)

Gene ablation in mice offers a powerful tool to assay in vivo the role of selected molecules. Numerous new mouse models of matrix metalloproteinases (MMP) deficiency have been developed in the past 5 years and have yielded a new understanding of the role of MMPs while also putting to rest assumptions based on data predating the days of mouse models. The phenotype of the MT1-MMP deficient mouse is one example which illustrates the sometimes rather surprising insights into extracellular matrix remodeling in development and growth that can be gained with mouse genetics. While MT1-MMP appears to play little or no role in embryonic development, loss of this enzyme results in progressive impairment of postnatal growth and development affecting both the skeleton and the soft connective tissues. The underlying pathologic mechanism is loss of an indispensable collagenolytic activity, which remains essentially uncompensated. Our findings demonstrate that growth and maintenance of the skeleton requires coordinated and simultaneous MT1-MMP-dependent remodeling of all soft tissue attachments (ligaments, tendons, joint capsules). We note that the phenotype of the MT1-MMP deficient mouse bears no resemblance to those of mice deficient in MMP-2 and tissue inhibitors of metallo-proteinase (TIMP)-2 all but dispelling the view that activation of MMP-2 by the MT1-MMP/TIMP-2/proMMP-2 axis plays a significant role in growth and development throughout life. It is of interest to note that loss of a single catabolic function such as selective collagen degradation mediated by MT1-MMP gives rise to profound impairment of a number of both anabolic and catabolic functions.
Pd6(.mu.3-CO)4(PMe3)7: the first octahedral hexapalladium cluster core (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPd6(.mu.3-CO)4(PMe3)7: the first octahedral hexapalladium cluster coreHans Friedrich Klein, Michael Mager, Ulrich Floerke, and Hans J. HauptCite this: Organometallics 1992, 11, 11, 3915–3917Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November 1992https://pubs.acs.org/doi/10.1021/om00059a069https://doi.org/10.1021/om00059a069research-articleACS PublicationsRequest reuse permissionsArticle Views123Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Dicarbonylrhodium(I) complexes of polypyrrole macrocycles. Part 3. Synthesis and crystal structures of complexes of N-methylcorroles, N-methylporphyrins, and acyclic polypyrroles (1979)

The reaction of di-µ-chloro-bis(dicarbonylrhodium) with N-methylcorroles gives stable out-of-plane complexes involving adjacent nitrogen atoms. An analogous complex is formed by a 21,24-dioxacorrole. The N-22-methylcorrole complex undergoes thermal equilibration with an isomeric complex involving non-adjacent nitrogen atoms. The X-ray crystal structures of two N-methylcorrolebis[dicarbonylrhodium(I)] complexes are reported. Reaction of di-µ-chloro-bis(dicarbonylrhodium) with N-methyletioporphyrin I gives rise to methylrhodium(III)–etioporphyrin and a similar reaction was observed with N-methylazaporphyrins. In the presence of an alkyl iodide, competitive alkylation of the rhodium occurs. Several examples of bis(dicarbonylrhodium) complexes of acyclic pyrrolic systems are reported including the first stable transition-metal complex of a 1, 19-dideoxy-1, 19-diunsubstituted biladiene-a, c.
A serine esterase released by human alveolar macrophages is closely related to liver microsomal carboxylesterases. (1991)

We identified a 60-kDa diisopropylfluorophosphate-(DFP) reactive protein in human bronchoalveolar lavage fluid, at a yield of 50-100 pmol/lavage. The protein is associated with the cell-free lavage fluid sediment, which consists mainly of surfactant. [3H]DFP labeling is inhibited by heating to 56 degrees C, 2 mM phenylmethylsulfonylfluoride and 1 mM bis(4-nitrophenyl)-phosphate. An identical 60-kDa [3H]DFP-reactive protein is present in the insoluble fraction of alveolar macrophage-conditioned culture medium and in total membrane preparations of alveolar macrophages. The [3H]DFP-labeled protein was purified approximately 30-fold from lavage fluid sediment by size-exclusion (Sephacryl S-200) and ion-exchange (Mono-Q) chromatography. Cyanogen bromide treatment of the partially purified protein produced a major labeled peptide of 14 kDa with an NH2-terminal sequence 90% identical to a region of form 1 rabbit liver microsomal carboxylesterase. Esterase activity in unlabeled starting material, detected using p-nitrophenyl valerate as substrate, copurified with the [3H]DFP-labeled enzyme. Degenerate oligonucleotide primers were designed based on the partial amino acid sequence and on a highly conserved region of known liver carboxylesterase sequences. Polymerase chain reaction using these primers and reverse-transcribed human alveolar macrophage mRNA yielded a 354-base pair product which was then used to screen a human alveolar macrophage cDNA library. A complete esterase sequence was obtained from two incomplete, overlapping clones, and is virtually identical to human liver carboxylesterase partial sequences. Northern blot analysis demonstrated a single approximately 1.7-kilobase transcript in human monocytes and alveolar macrophages, with much higher levels in the latter. These data indicate that human alveolar macrophages both contain and release a serine esterase that is apparently identical to liver microsomal carboxylesterase. Its enzymatic profile suggests it is a major component of alveolar macrophage-nonspecific esterase activity. We hypothesize that it acts as a detoxication enzyme in the lung.
Immunohistochemical Profile of Human Pancreatic Pacinian Corpuscles (2010)

Pancreatic pacinian corpuscles slightly differ from the cutaneous ones, and these differences could be related to topography, growth factor requirements, or function of pacinian corpuscles in the pancreas.
Modification of AISI M2 high-speed tool steels after laser-surface melting under different operating conditions (2011)

We applied a laser-surface melting treatment to AISI M2 high-speed steel – hardened and tempered – and studied the resulting surface characteristics (microstructure) and mechanical behaviour (hardness and wear performance). The steel was treated using a Nd:YAG continuous-wave laser with different operation conditions. The influence of the laser processing parameters on the individual beads and on the characteristics of the molten surface layer obtained using the multipass system with 50% overlap was studied. The microstructure characterization for all conditions is formed of MC- and M2C-type carbides, martensite, and retained austenite; the quantities of this phase depend on the operating conditions. It has been determined that low levels of power density and high scanning speeds of the beam lead to greater homogeneity in the microstructure with high hardness values and wear resistance.
6—A COMPARISON OF NEW SYNTHETIC PYRETHROIDS FOR THE INDUSTRIAL INSECTPROOFING OF WOOL (1979)

An investigation is reported in which five new synthetic pyrethroids were evaluated in the laboratory for their effectiveness as durable insectproofing agents for wool. Their dyebath-exhaustion behaviour was examined under four sets of conditions. The fastness of the treatments to washing, dry-cleaning, and light was assessed by chemical analysis and assay against insects. Four of the compounds studied, WL–43479 (permethrin 40:60 cis:trans), NRDC–143 (permethrin 25:75 cis:trans), S–3206 (fenpropanate), and S–5602 (fenvalerate), showed sufficient promise to warrant further investigation of their use as industrial insectproofing agents.
Chemotaxis of Bdellovibrio bacteriovorus toward pure compounds (1979)

Positive chemotaxis by Bdellovibrio bacteriovorus strain UKi2 was measured for 139 compounds. Twenty-one compounds were attractants; sensitive attraction was elicited by acetate, propionate, thioacetate, malonate, cis-oxalacetate, D-glucose-6-phosphate, acetyl coenzyme A, ammonium ion, barium ion, manganous ion, and potassium ion. Several of the attractants for B. bacteriovorus strain UKi2 also were attractants to strains 6-5-S and 114; however, strains 109D and 109J were not attracted by the compounds tested. Of 33 compounds tested, 8 were repellents for B. bacteriovorus strain UKi2: n-caproate, alanine, isoleucine, leucine, phenylalanine, tyrosine, cobaltous chloride, and hydronium ion. None of the organic repellents for strain UKi2 elicited repulson from strains 114 or 109D. However, all three strains of Bdellovibrio show aerotaxis. Several compounds were tested for their effects on viability and predacious growth of B. bacteriovorus strain UKi2. No simple correlation was found between attraction or repulsion and benefit or harm to bdellovibrios. The data are consistent with the view that in nature, the greatest survival value of chemotaxis for bdellovibros may be in aerotaxis, attraction to certain inorganic ions and acetate, and repulsion by hydronium ion.
Convergence of p53 and Transforming Growth Factor β (TGFβ) Signaling on Activating Expression of the Tumor Suppressor Gene maspin in Mammary Epithelial Cells (2007)

Using two-dimensional difference gel electrophoresis, we identified the tumor suppressor gene maspin as a transforming growth factor beta (TGFbeta) target gene in human mammary epithelial cells. TGFbeta up-regulatesMaspin expression both at the RNA and protein levels. This up-regulation required Smad2/3 function and intact p53-binding elements in the Maspin promoter. DNA affinity immunoblot and chromatin immunoprecipitation revealed the presence of both Smads and p53 at the Maspin promoter in TGFbeta-treated cells, suggesting that both transcription factors cooperate to induce Maspin transcription. TGFbeta did not activate Maspin-luciferase reporter in p53-mutant MDA-MB-231 breast cancer cells, which exhibit methylation of the endogenous Maspin promoter. Expression of ectopic p53, however, restored ligand-induced association of Smad2/3 with a transfected Maspin promoter. Stable transfection of Maspin inhibited basal and TGFbeta-stimulated MDA-MB-231 cell motility. Finally, knockdown of endogenous Maspin in p53 wild-type MCF10A/HER2 cells enhanced basal and TGFbeta-stimulated motility. Taken together, these data support cooperation between the p53 and TGFbeta tumor suppressor pathways in the induction of Maspin expression, thus leading to inhibition of cell migration.
Spectroscopic Optical Coherence Tomography for Thin Layer and Foil Measurements (2020)

The main goal of this research was to assess if it is possible to evaluate the thickness of thin layers (both thin films on the surface and thin layers below the surface of the tested object) and foils using optical coherence tomography (OCT) for thickness assessment under the resolution of the standard commercially available OCT measurement system. In the proposed solution, light backscattered from the evaluated thin layer has been expressed as a multiple beam interference. Therefore, the OCT system was modeled as a two-beam interferometer (e.g., Michelson), in which one beam propagates from the reference arm and the other comes from a Fabry-Pérot interferometer. As a consequence, the mathematical model consists of the main Michelson interferometer, in which the measuring arm represents the Fabry-Pérot interferometer. The parameters of the layer (or foil) are evaluated by analyzing the minimum value of the interference contrast. The model developed predicts the behavior of the thin layers made from different materials (with different refractive indexes) with different thickness and located at different depths. To verify the correctness of the proposed model, an experiment with a wedge cell has been carried out. The wedge cell was shifted across the scanning beam using a linear translation stage with a micrometer screw under the scanning head. The relationship between the thickness of the gap of the wedge cell and the OCT output signal is presented. For the additional verification of the proposed model, the results of the measurements of the thickness of the thin foil were compared with the theoretical results of the simulations. The film thickness was evaluated based on the calculated positions of the minimum value of interference contrast. A combination of the standard potentialities of OCT with the proposed approach to analyzing the signal produces new metrological possibilities. The method developed allows us to evaluate thickness under the resolution of the system and the location of the layer as well. This produces the possibility of measuring a layer which is covered by another layer. Moreover, it is possible to create a thickness map with high sensitivity to thickness changes. These experiments and simulations are the culmination of preliminary research for evaluating the potential of the proposed measurement method.
Supplementary Figure 3 from Ganetespib (STA-9090), a Nongeldanamycin HSP90 Inhibitor, Has Potent Antitumor Activity in <i>In Vitro</i> and <i>In Vivo</i> Models of Non–Small Cell Lung Cancer (2023)

&lt;p&gt;PDF file, 365K, Activity of ganetespib in HCC827 xenografts.&lt;/p&gt;