gebhart/patrepeval huggingface dataset:
Assessment of One- and Two-Photon Excited Luminescence for Directly Measuring O2, pH, Na+, Mg2+, or Ca2+ in Optically Dense and Biologically Relevant Samples (2002)

We compare the emission spectra and analytical response profiles of several semiselective luminescent probes that are commonly used to quantify nonfluorescent analytes (ruthenium(II) tris(2,2′-bipyridyl) dication, tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dication, SNARF®-1, fluorescein, Rhodol Green™, and Sodium™, Magnesium™, and Calcium Green™) when they are excited under one- and two-photon excitation conditions in water, aqueous solutions of dyes or fluorophores, undiluted mouse or human serum, human urine, and mouse whole blood. The results from this work can be summarized as follows. First, in cases where the probes possess ground states that are composed of multiple species in equilibrium (e.g., acid and base forms of a luminophore), the analyte-dependent emission spectra can be significantly different under one- and two-photon excitation conditions due to differences in the relative one- and two-photon cross-sections associated with the individual ground-state species. Second, for those probes that exhibit one- and two-photon dependent emission spectra, an assessment of the analytical response vs. target analyte concentration profiles show that the response dynamic range can be improved by up to 100 fold, but the response sensitivity can decrease by up to 25% for two- vs. one-photon excitation. Third, two-photon excited luminescence provides a means to use these semiselective luminescent probes to quantify nonfluorescent analytes directly within optically dense mixtures, including solutions of dyes, mouse or human serum, human urine, and mouse whole blood. Finally, two-photon excited luminescence allows one to quantify nonfluorescent analytes in a spatially defined manner within complex samples.
Load to voltage dependency tests at B.C. Hydro (1995)

This paper describes the methodology, design, and results of a measurement-based determination of the load to voltage dependencies (also described as load sensitivities to voltage change) for a B.C. Hydro substation selected for a distribution automation pilot project. The test methodology was based on using data from a "control" substation, which represented the "natural" variation of the load at the test substation. The measured data was processed using correlation and multi-regression analysis. The tests were performed on a customer base which is mainly residential with some commercial, and a common high penetration of resistive-load. Spring and summer sets of measurements were gathered, and different load to voltage sensitivities for different seasons and time of day were derived. The difference between the sensitivity of the load at the measurement site and at the customer terminals was determined by using a detailed operation model.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Subsurface‐Carbon‐Induced Local Charge of Copper for an On‐Surface Displacement Reaction (2021)

Abstract Transition‐metal carbides have sparked unprecedented enthusiasm as high‐performance catalysts in recent years. Still, the catalytic properties of copper carbide remain unexplored. By introducing subsurface carbon to Cu(111), a displacement reaction of a proton in a carboxyl acid group with a single Cu atom is demonstrated at the atomic scale and room temperature. Its occurrence is attributed to the C‐doping‐induced local charge of surface Cu atoms (up to +0.30 e/atom), which accelerates the rate of on‐surface deprotonation via reduction of the corresponding energy barrier, thus enabling the instant displacement of a proton with a Cu atom when the molecules adsorb on the surface. This well‐defined and robust Cu δ+ surface based on subsurface‐carbon doping offers a novel catalytic platform for on‐surface synthesis.
[Glycerol electrocochleographic test: role of endolymphatic sac?]. (1996)

The administration of a dehydrating agent like glycerol may improve the audiometric thresholds and decrease the summating potential in the electrocochleographic recordings of some Méière's diseases. There are many discussions concerning the endolymphatic sac's rôle in this cases. To clarify this subject we have reviewed the labyrinth's liquids physiopathology, the structural observations in cases of Ménière's diseases, the animals models date. We conclude that the presence of an endolymphatic sac is not necessary for obtaining a positive glycerol test. The clinical meaning of this test is discussed.
Supplementary Figure S3 from Lurbinectedin Inactivates the Ewing Sarcoma Oncoprotein EWS-FLI1 by Redistributing It within the Nucleus (2023)

&lt;p&gt;Supplementary Figure S3 Single-cell imaging of HA-tagged TC32 cells treated with 2.5 nM trabectedin or lurbinectedin for 6 h shows EWS-FLI1 (green) localized into the nucleolus (red). DAPI (blue) staining of the nucleus.&lt;/p&gt;
Binding of a cementum attachment protein to extracellular matrix components and to dental surfaces (1992)

Cementum proteins (CP) have been shown to mediate cell attachment. Among these, a 55 kDa protein was isolated. The purpose of the present study was to assess the capacity of CP to bind to non-demineralized and demineralized root surfaces and to support cell attachment to dentin. CP were prepared by sequential extraction of bovine cementum with 25 mM EDTA, 0.5 M acetic acid followed by 4 M guanidine HCl. The latter was subjected to ion exchange chromatography on a DEAE-3SW column and eluted stepwise with a 0-0.5 M NaCl gradient. CP were labelled with 125I and the capacity of 125I-CP to bind to mineralized and partially demineralized dentin, synthetic hydroxyapatite, collagen, fibronectin and fibrillar collagen-fibronectin complex was assessed. It was found that CP bind specifically to mineralized dentin and synthetic hydroxyapatite but not to demineralized dentin. The specific binding was 60% of the total binding. SDS-PAGE analysis of the proteins bound to dentin indicated that the main bound protein had a molecular weight of 55 kDa. CP exhibited high affinity for fibronectin (kD = 1.56 x 10(-10) M) and fibronectin-collagen complex, but their binding to either molecular or fibrillar collagen was negligible. It is suggested that CP may play an important role in the attachment of cells of the periodontium to cementum extracellular matrix during homeostasis and regeneration.
SPECT imaging of dopamine D2 receptors with 2'-iodospiperone. (1995)

2'-ISP can be used for mapping dopamine D2 receptors in human brain with SPECT. The relatively high background activity, however, suggests that further modification of the compound may be needed prior to widespread clinical use.
Presence of Calcitonin-Like Peptide in Rat Milk: Possible Physiological Role in Regulation of Neonatal Prolactin Secretion* (1989)

Previous results have shown that salmon calcitonin (sCT), a peptide in rat brain and pituitary gland, inhibits basal and TRH-stimulated PRL release and reduces PRL mRNA levels in isolated anterior pituitary cells of adult rats in culture. Rat milk contains a variety of neuropeptides and hormones, some of which are absorbed in bioactive form to exert endocrine influences in the developing offspring. The present studies were undertaken to investigate whether a CT-like peptide is present in rat milk. Circulating PRL levels in neonatal rats are low, and there is an abrupt increase in the basal secretion of this hormone at weaning. A second objective was to examine whether CT plays a role in the regulation of PRL secretion in neonatal animals. A sensitive and specific RIA for sCT was developed and used to assay rat milk on various days of lactation for sCT-like immunoreactivity. sCT-like activity was present in the water-soluble (infranatant) fraction of milk throughout lactation in concentrations as high as 1589 pg/ml. There were no statistically significant differences in immunoreactive levels of the peptide in milk samples from different days of lactation. sCT-like immunoreactivity in rat milk infranatant coeluted with synthetic sCT on reverse phase HPLC, and these HPLC fractions inhibited basal PRL release when added to cultures of anterior pituitary cells. This inhibition of PRL release by the sCT-immunoreactive HPLC fractions was comparable to that exerted by equivalent concentrations of synthetic sCT. Newborn rats were injected sc with 10 microliters normal rabbit serum or anti-sCT serum from the day of birth until postpartum day 10. The rats were killed on day 11, and their sera were analyzed for PRL. Anti-sCT-injected rats showed a significant increase in serum PRL levels compared to those in untreated or normal serum-treated rats. These results demonstrate that a CT-like peptide, which is a potent inhibitor of PRL release, is present in rat milk throughout lactation, and that passive immunization with a highly specific anti-sCT serum leads to an increase in serum PRL levels in neonatal rats. CT, possibly of milk origin, may be a physiologically relevant PRL-inhibiting factor during the neonatal period.
Unit commitment for operations (1985)

The topic of unit commitment has been and continues to be of interest to many researchers and is a primary operation for most utilities. Past research has utilized integer programming, dynamic programming, linear programming, gradient, and heuristic techniques. This research combines both linear programming and dynamic programming for unit commitment decisions within a weekly time frame. The result provides most of the advantages of linear programming and dynamic programming with less stringent requirements on the pre solution information needed for unit transition sequences. Further, the research yields a new tool for the solution of the Transaction Evaluation problem.
Reduced pain perception with Pen MateTM an automatic needle insertion device for use with an insulin pen (1999)

Abstract The study examined glycaemic control and pain perception in patients using NovoPen® 3 with or without PenMate TM , an automatic injection device, in an open, cross‐over trial. Patients (n=57), who had been injecting insulin for at least 12 months and who had been using Novo Pen®3 for at least six weeks prior to the study, were randomised to use Novo Pen®3 with or without Pen Mate TM for six weeks, before crossing over to the alternate system for a further six weeks. Pain perception was recorded night and morning on a 100mm visual analogue scale (VAS). Glycaemic control was monitored by HbA 1c measurements and by 4‐point blood glucose profiles. Pain perception was significantly lower with PenMate TM (p=0.005). No statistical difference in HbA 1c , blood glucose profiles or adverse events were found between the two systems. Patients found PenMate TM easy to use and 84% said they would recommend the device to a person who is uncomfortable with needles.
Benzothiazoles - Scaffold of Interest for CNS Targeted Drugs (2015)

Benzothiazole compounds represent heterocyclic systems comprising a benzene ring fused with a thiazole ring containing nitrogen and sulphur in its structure. Besides the presence of a benzothiazole core in naturally occurring molecules, synthesized compounds containing a benzothiazole moiety in their structure proved to be a significant class of potential therapeutics, as they exhibit biological effects such as antitumor, antibacterial, antitubercular, antiviral, anthelmintic, antidiabetic and many others. Apart from the aforementioned peripheral or microbial active sites, benzothiazole analogues are also biologically active compounds in the central nervous system, where some approved drugs containing a benzothiazole moiety have already been identified and are used in the treatment of various neurological disorders. New benzothiazole molecules are currently under development and are being evaluated for several uses including diagnostics and as therapeutic drug candidates for the treatment of epilepsy and neurodegenerative diseases such as Alzheimer's disease, Huntington's disease and amyotrophic lateral sclerosis amongst others.
Optimized Viral Dose and Transient Immunosuppression Enable Herpes Simplex Virus ICP0-Null Mutants To Establish Wild-Type Levels of Latency In Vivo (2000)

The reduced efficiency with which herpes simplex virus type 1 (HSV-1) mutants establish latent infections in vivo has been a fundamental obstacle in efforts to determine the roles of individual viral genes in HSV-1 reactivation. For example, in the absence of the "nonessential" viral immediate-early protein, ICP0, HSV-1 is severely impaired in its ability to (i) replicate at the site of inoculation and (ii) establish latency in neurons of the peripheral nervous system. The mouse ocular model of HSV latency was used in the present study to determine if the conditions of infection can be manipulated such that replication-impaired, ICP0-null mutants establish wild-type levels of latency, as measured by viral genome loads in latently infected trigeminal ganglia (TG). To this end, the effects of inoculum size and transient immunosuppression on the levels of acute replication in mouse eyes and of viral DNA in latently infected TG were examined. Following inoculation of mice with 2 x 10(3), 2 x 10(4), 2 x 10(5), or 2 x 10(6) PFU/eye, wild-type virus replicated in mouse eyes and established latency in TG with similar efficiencies at all four doses. In contrast, increasing the inoculum size of the ICP0-null mutants n212 and 7134 from 2 x 10(5) to 2 x 10(6) PFU/eye significantly decreased the levels of infectious virus detected in the tear films of mice from days 4 to 9 postinfection. In an attempt to establish the biological basis for this finding, the effect of viral dose on the induction of the host proinflammatory response was examined. Quantitative reverse transcription-PCR demonstrated that increasing the inoculum of 7134 from 2 x 10(4) to 2 x 10(6) PFU/eye significantly increased the expression of proinflammatory (interleukin 6), cell adhesion (intercellular adhesion molecule 1), and phagocyte-associated (CD11b) genes in mouse eyes 24 h postinfection. Furthermore, transient immunosuppression of mice with cyclophosphamide, but not cyclosporin A, significantly enhanced both the levels of acute n212 and 7134 replication in the eye and the levels of mutant viral genomes present in latently infected TG in a dose-dependent manner. Thus, the results of this study demonstrate that acute replication in the eye and the number of ICP0-null mutant genomes in latently infected TG can be increased to wild-type levels for both n212 and 7134 by (i) optimization of inoculum size and (ii) transient immunosuppression with cyclophosphamide.
IL‐1Ra deficiency accelerates intervertebral disc degeneration in C57BL6J mice (2022)

The expression of Interleukin-1ß (IL-1ß) and its antagonist and Interleukin-1 receptor antagonist (IL-1Ra) are correlated with greater human intervertebral disc (IVD) degeneration, suggesting that elevated IL-1β activity promotes disc degeneration. Many in vitro studies support such a mechanistic relationship, whereas few in vivo investigations have been reported. The present study tests the effect of increased IL-1β activity on intervertebral disc in mice with an IL-1Ra gene deletion. IL-1Ra-/- mice and wild-type (WT) C57Bl6J mice were examined at 3 and 12 months of age. Caudal IVD segments were evaluated for disc degeneration by histopathology, functional testing, and inflammatory gene expression relevant to IL-1β pathways. To test differences in injury response, pinprick annular puncture was performed on IL-1Ra-/- and WT mice and evaluated similarly. IL-1Ra-/- IVDs had significantly worse histopathology at 3 months compared to WT controls, but not at 12 months. IL-1Ra-/- IVDs exhibited significantly more viscous mechanical properties than WT IVDs. qPCR revealed downregulation of inflammatory genes at 3 and 12 months in IL-1Ra-/- IVDs, with concomitant downregulation of anabolic and catabolic genes. Annular puncture yielded no appreciable differences between 2-week and 6-week post-injured WT and IL1-Ra-/- IVDs in histopathology or biomechanics, but inflammatory gene expression was sharply downregulated in IL-1Ra-/- mice at 2 weeks, returning by 6 weeks post injury. In the present study, IL-1Ra deletion resulted in increased IVD histopathology, inferior biomechanics, and transiently decreased pro-inflammatory cytokine gene expression. The histopathology of IL-1Ra-/- IVDs on a C57BL/6J background is less severe than a previous report of IL1Ra-/- on a BALB/c background, yet both strains exhibit IVD degeneration, reinforcing a mechanistic role of IL-1β signaling in IVD pathobiology. Despite a pro-inflammatory environment, the annular puncture was no worse in IL-1Ra-/- mice, suggesting that response to injury involves pathways other than inflammation. Overall, this study supports the hypothesis that IL-1β-driven inflammation is important in IVD degeneration.
Multicenter, phase II clinical trial of peptide vaccination with oral chemotherapy following curative resection for stageï¿½III colorectal cancer (2018)

The safety and immunological responsiveness of a peptide vaccine of ring finger protein 43 and 34-kDa translocase of the outer mitochondrial membrane combined with uracil-tegafur/leucovorin (UFT/LV) was previously demonstrated in metastatic colorectal cancer (CRC) in a phase I clinical trial. To clarify the survival benefit of a peptide vaccine combined with UFT/LV as adjuvant treatment, a phase II clinical trial was conducted involving patients with stage III CRC. All enrolled patients, whose human leukocyte antigen (HLA)-A status was double-blinded, were administered the same regime of a peptide vaccine and UFT/LV chemotherapy. The primary objective of the study was to compare relapse-free survival (RFS) in patients with HLA-A*2402 vs. those without HLA-A*2402. Secondary objectives included comparisons between the two groups regarding overall survival, safety, tolerability and peptide-specific activities of cytotoxic T lymphocytes (CTLs) as measured by the ELISPOT assay. Between December 2009 and December 2014, a total of 46 patients were enrolled to the present study. Three-year RFS was not significantly different between HLA-A*2402 matched and unmatched groups [67.8 vs. 73.6%, respectively; hazard ratio (HR)=1.254, 95% confidence interval (CI): 0.48-4.63; P=0.706]. Three-year RFS was significantly better in patients with positive CTL responses in the HLA-A*2402 matched group compared with those without (85.7 and 33.3%, respectively; HR=0.159, 95% CI: 0.023-0.697; P=0.011). In conclusion, vaccination-induced immune responses combined with UFT/LV were positively associated with survival benefit in patients with HLA-A*2402-positive stage III CRC. Further study is required to clarify whether vaccination-induced immune responses shortly following the initiation of therapy can predict the therapeutic effect and help develop a promising therapeutic strategy for patients with stage III CRC.
Study of the fine structure of adeno-associated virus DNA with bacterial restriction endonucleases (1975)

A physical map of the adeno-associated virus type 2 genome has been constructed on the basis of the five fragments produced by the restriction endonucleases HindII + III from Hemophilus influenzae. There are three endo R-HindII cleavage sites and one endo R-HindIII site. Evidence has been obtained to support the existence of two nucleotide sequence permutations in adeno-associated virus DNA, the start points of which have been estimated to be separated by 1% of the genome. The three cleavage fragments produced by endo R-Eco RI have been ordered and oriented with respect to the endo R-HindII + III cleavage map.
A 65 nm CMOS Inductorless Triple Band Group WiMedia UWB PHY (2009)

A fully integrated WiMedia v1.2 UWB PHY (radio plus digital baseband) covering all TFC codes has been realized in a baseline 65 nm CMOS process. The PHY features also detect and avoidance (DAA) capability. The inductorless design uses a single RF signal path to cover band groups 1, 3 and 6. Per band group only 2 fast settling all-digital PLLs generate the three required LO frequencies in a so-called `ping-pong' configuration. Each band group uses dedicated digitally controlled <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">RC</i> ring-based oscillators. The EVM for 480 MBps is below -24 dB and the sensitivity is below -81 dBm for 53 Mb/s. The IIP3 for maximum gain setting is better than -4 dBm. For the 480 Mb/s data rate the PHY draws 383 mW in receive mode and 312 mW in transmit mode from 1.2 V supply. The SoC measures 3.3×3.3 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
Pharmacodynamics of fish oil: protective effects against prostate cancer in TRAMP mice fed with a high fat western diet. (2011)

This is the first study showing the beneficial effects of FO against prostate cancer having a HF diet, suggesting potential beneficial effects of FO in humans consuming HF in their diet.
Is the Enzyme ACMSD a Novel Therapeutic Target in Parkinson’s Disease? (2017)

Several large genome wide association studies have identified a locus in close proximity to the gene encoding the enzyme aminocarboxymuconate-semialdehyde-decarboxylase (ACMSD) to be associated with the risk for Parkinson's disease (PD), tentatively suggesting that this enzyme might influence PD pathogenesis. Further support for this comes from the recent identification of a disease-segregating stop codon mutation in ACMSD in a family with Parkinsonism, and a missense mutation in the ACMSD gene predicted to disrupt enzyme function in an individual with typical PD. ACMSD is part of the kynurenine pathway, responsible for the catalytic breakdown of tryptophan into NAD+, generating several neuroactive metabolites in the process. The enzyme is located at a key branch-point of the pathway, limiting the production of the neurotoxin quinolinic acid, which has excitotoxic and inflammatory properties. In this review, we discuss the genetic findings in light of the functions of ACMSD and its potential involvement in PD pathogenesis.
Improvement of photovoltaic properties in inorganic organic pigment particle heterojunction solar cell (2012)

Oxytitanium phthalocyanine (TiOPc) and zinc oxide (ZnO) pigments working as photoactive layers have been used to fabricate pigment particle hetero-junction structured solar cells. Performance test with this double layer cell shows an energy conversion around 0.15%. On the other hand, triple layered solar cell where TiOPc is sandwiched between H 2 Pc (metal-free phthalocyanine) and ZnO shows an efficiency of about 2.4%. In both cases, good life test results have been obtained. Dependences of photovoltaic characteristics on the structure of double and triple layered devices, comprised of semiconductor pigment particles are shown and their potential for future application has been discussed.
A New Approach to Dendrimers made fromp-tert-Butyl Calix[4]arenes (2006)

A new family of hyperbranched molecules and dendrimers has been constructed from a diamide–dicalix derivative prepared from monocarbomethoxymethyl p-tert-butyl calix[4]arene and tris(2-aminoethyl)amine (‘tren’) via amide-formation reactions. The selective 1,3-di-O-functionalization of p-tert-butyl calix[4]arene moieties allows the synthesis of first- (G1) and second-generation (G2) calix-dendrimers. Replacement of the quadridentate amine by a trithia-ether-triamine-mono-ol, ’hyten’, again results in acylation of the amino groups, but with the generation of a central cavity with different complexing properties. A new family of hyperbranched molecules and dendrimers has been constructed from a diamide–dicalix derivative prepared from monocarbomethoxymethyl p-tert-butyl calix[4]arene and tris(2-aminoethyl)amine (‘tren’) via amide-formation reactions. The selective 1,3-di-O-functionalization of p-tert-butyl calix[4]arene moieties allows the synthesis of first- (G1) and second-generation (G2) calix-dendrimers