gebhart/patrepeval huggingface dataset:
97 Dynamic diffuse optical spectroscopy can diagnose and quantify lupus arthritis (2019)

<h3>Background</h3> SLE arthritis is difficult to evaluate because of the sometimes-evanescent nature of the symptoms and limitations of physical exams and imaging studies. Dynamic diffuse optical spectroscopy (dDOS) can be used to assess changes in light absorption through tissues during transient venous occlusion. The optical signal reflects changes in blood perfusion and has diagnostic value in rheumatoid arthritis. The current study explored the use of dDOS in SLE arthritis. <h3>Methods</h3> 12 SLE patients (ACR criteria) with active arthritis and 5 controls were evaluated. A dDOS sensor module was developed (figure 1a). Hemodynamic effects were obtained by inflating a BP cuff to 40 mmHg ×60 s. Light at 3 wavelengths (lambda=530, 655, 940 nm) was used to illuminate joints at 8 different points. Transmitted light intensities were measured with Si-photodetectors at 8 other positions (total 8×8×3=192 signal traces). Swollen, tender and healthy joints were examined by the same assessor. <h3>Results</h3> SLE patients and normal controls dDOS data were available for analysis from 66 and 24 proximal interphalangeal (PIP) joints, respectively (PIPs 2–4). Best results were obtained at 530 nm with cuff inflation at 40 mmHg. A representative measurement of 3 SLE arthritis and 3 normal joints is shown in figure 1b, highlighting differences in rise and plateau time. Given the pronounced effects at lambda=530 nm, we speculate that altered vessel physiology paired with already-increased blood pooling in the affected inflamed joints resulted in quicker increase in light absorption (rise time) that is maintained longer (plateau time) compared to normal joints. The AUC for dDOS was consistent with excellent discrimination, AUC=0.8639, sensitivity=76.19, specificity=88.57 (figure 1c). <h3>Conclusions</h3> dDOS can evaluate SLE arthritis with high sensitivity and specificity. Rise and plateau time of the optical traces correlate strongly with swollen and tender joint count. The advantages of dDOS are non-invasiveness, objectivity (eliminates inter-rater variability and operator dependency), low cost, and high speed of performance (~5 min per area of scanning) compared to US and MRI. dDOS has the potential to bring much-needed objectivity to the quantification of SLE arthritis. <h3>Funding Source(s):</h3> N/A
Expression of Recombinant Insulin-Like Growth Factor-Binding Protein-3 Receptor in Mammalian Cell Line and Prokaryotic (Escherichia coli) Expression Systems (2022)

We designed a new experiment in the h6-TMEM219 expression procedure in both eukaryotic and prokaryotic hosts. All of our results confirm appropriate transformation and transfection and importantly, approve h6-TMEM 219 membrane expression. Finally, the HeLa h6-TMEM219 cells and the newly purified h6-TMEM219 leverage new studies for molecular diagnostic studies and characterize the therapeutic agents against IGFBP-3/TMEM219 signalling pathway in devastating illnesses in vitro and in vivo.
Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction (1996)

Detection of chlamydial DNA in the joints of patients by PCR gives positive results more often when synovial tissue rather than SF is the source of target nucleic acids. Although synovial tissue is the source of choice for the most reliable determination of chlamydia in the joint, both synovial tissue and SF should be assayed if possible.
Synthesis and Evaluation of Novel Pyrimido‐Acridone, ‐Phenoxadine, and ‐Carbazole as Topoisomerase II Inhibitors. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Se acabaron las citologías con discariosis moderada? (2009)

Objetivo. La British Society for Clinical Cytology ha propuesto recientemente una modificación de la terminología para los informes de frotis cervicales, pasando de un sistema de 3 tipos (discariosis leve, moderada o grave) a otro de dos tipos, denominados discariosis de bajo grado y de alto grado. Esta modificación elimina la categoría central de discariosis moderada que se incorporaría al grupo de alto grado. El objetivo de este estudio fue investigar el papel de las citologías con discariosis moderada en la práctica clínica. Material y métodos. Se llevó a cabo una revisión retrospectiva de todas las mujeres que fueron remitidas a una exploración colposcópica debido a una citología con discariosis moderada a lo largo de un periodo de 6 meses. Los datos recogidos incluyeron la impresión colposcópica, la intervención realizada y el diagnóstico histopatológico final. Se pidió a dos citólogos, que no conocían el informe inicial de las citologías, que volvieran a clasificarlos con el empleo del nuevo sistema de sólo dos clases. Se compararón sus resultados con los resultados documentados de la colposcopia e histopatología. Resultados. Un total de cien mujeres con citologías con discariosis moderada fueron remitidas a colposcopia durante el período de estudio. La mayoría de ellas fueron clasificadas en la categoría de discariosis de alto grado utilizando el nuevo sistema. En 56 (72%) de las citologías con discariosis moderada que fueron clasificados correctamente como de alto grado por el citólogo 1 se observó una neoplasia cervical intraepitelial 2-3 en la histopatología final, mientras que en el caso del citólogo 2, en 66 (68%) se observó una neoplasia cervical intraepitelial de alto grado. Conclusión. La conservación del término de discariosis moderada no aporta ninguna ventaja clínica. Este estudio resalta la necesidad de utilizar un sistema uniforme de dos clases.
Nephrolithiasis: What Surgeons Need to Know (2011)

Nephrolithiasis is a common cause of morbidity with a lifetime prevalence of 5-10% worldwide. Increasingly, diagnostic evaluation and planning for medical or surgical intervention have become reliant on imaging.
A COMPARISON OF ELECTROCARDIOGRAPHIC AND ECHOCARDIOGRAPHIC ABNORMALITIES IN SUBARACHNOID HEMORRHAGE (1990)

Log in or Register Subscribe to journalSubscribe Get new issue alertsGet alerts Enter your Email address: Wolters Kluwer Health may email you for journal alerts and information, but is committed to maintaining your privacy and will not share your personal information without your express consent. For more information, please refer to our Privacy Policy. Subscribe to eTOC Secondary Logo Journal Logo All Articles Images Videos Podcasts Blogs Advanced Search Toggle navigation Subscribe Register Login Articles & Issues Current IssuePrevious IssuesPublished Ahead-of-Print Collections SNACC Clinical Guidelines and Consensus StatementsSNACC Abstracts For Authors Submit a ManuscriptInformation for AuthorsLanguage Editing ServicesAuthor Permissions Journal Info About the JournalEditorial BoardAffiliated SocietiesAdvertisingOpen AccessSubscription ServicesReprintsRights and Permissions All Articles Images Videos Podcasts Blogs Advanced Search
Carnitine transport in rat heart slices: II. The carnitine/deoxycarnitine antiport. (1985)

In rat heart slices carnitine transport occurs in an exchange process with deoxycarnitine. This has been demonstrated in double labelling experiments allowing a preloading of either 3H-carnitine or 14C-deoxycarnitine, the immediated precursor of carnitine. The stoichiometry of the carnitine/deoxycarnitine exchange resulted close to one in both directions. The relative kinetics supports the assumption that the process is mediated by a membrane bound protein. The results may rationalize the circumstance that carnitine is taken up by myocardium against a concentration gradient. The meaning of the carnitine/deoxycarnitine exchange is discussed.
Review. Travaux de Linguistique et de Litterature, publ. par le Centre de Philologie et de Litteratures Romanes de l'Universite de Strasbourg, Vol. V, 1 (1969)

REVIEWS Travaux de Linguistique et de Littérature. Publiés par le Centre de Philologie et de Littératures Romanes de l'Université de Strasbourg. Vol. V, 1. Strasbourg: Centre de Philologie et de Littératures Romanes; Paris: Klincksieck. 1967. 233 pp STEPHEN ULLMANN STEPHEN ULLMANN OXFORD Search for other works by this author on: Oxford Academic Google Scholar French Studies, Volume XXIII, Issue 2, April 1969, Pages 216–218, https://doi.org/10.1093/fs/XXIII.2.216 Published: 01 April 1969
Requirement for Formin-Induced Actin Polymerization during Spread of Shigella flexneri (2009)

Actin polymerization in the cytosol and at the plasma membrane is locally regulated by actin nucleators. Several microbial pathogens exploit cellular actin polymerization to spread through tissue. The movement of the enteric pathogen Shigella flexneri, both within the cell body and from cell to cell, depends on actin polymerization. During intercellular spread, actin polymerization at the bacterial surface generates protrusions of the plasma membrane, which are engulfed by adjacent cells. In the cell body, polymerization of actin by Shigella spp. is dependent on N-WASP activation of the Arp2/Arp3 complex. Here we demonstrate that, in contrast, efficient protrusion formation and intercellular spread depend on actin polymerization that involves activation of the Diaphanous formin Dia. While the Shigella virulence protein IpgB2 can bind and activate Dia1 (N. M. Alto et al., Cell 124:133-145, 2006), its absence does not result in a detectable defect in Dia-dependent protrusion formation or spread. The dependence on the activation of Dia during S. flexneri infection contrasts with the inhibition of this pathway observed during vaccinia virus infection.
Iodine doping in solid precursor-based CVD growth graphene film (2011)

Doping of different elements in intrinsic graphene is of great importance to adjust the electrical and chemical properties for realization of different electronic devices. Here, we demonstrate a simple and controllable synthesis process of iodine-doped graphene film using camphor (C10H16O), a solid botanical derivative. In situ doping of iodine in a graphene film has many difficulties in a conventional chemical vapor deposition process using a gas source. In this technique, iodine was mixed with the carbon precursor and simultaneously evaporated to pyrolysis on a metal catalytic substrate. Raman and X-ray photoelectron spectroscopic studies confirm the presence of elemental iodine in the form of triiodide and pentaiodide. Simultaneously, evaporated iodine atoms remains within the few-layers graphene structure and interact with carbon atoms through a charge transfer process. This shows a straightforward technique for iodine doping in graphene and a similar approach can be adopted to deposit iodine-doped graphene on other metal substrates.
Measurements During Drilling Through an Innovative Microchip Technology to Determine Accurate Wellbore Properties for Efficient Drilling Operations (2021)

Abstract The measurement of the drilling parameters such as temperature and pressure helps mitigate drilling-related issues and optimize drilling operations on a cost-effective basis. Multiple technologies can measure these parameters; however, the current tools suffer from low bandwidth, associated high cost, and limited measurement locations near the drill bit. This reduced accuracy and transmission rate while drilling can be improved using intelligent microchip tracers and micro-memory balls. These tools can measure the temperature and pressure across an entire wellbore. The proposed tracers include a microprocessor-based circuit board equipped with sensors, a communication antenna, and a rechargeable battery, all protected from the harsh downhole environment through a robust composite material. The advanced microchip tracers and micro-memory ball technologies were tested in the field and provided innovative measurement platforms. The field tests were conducted in various environments, including oil and gas wells, deviated wells, multiple hole sizes, varied fluid densities, and different BHA (Bottom Hole Assembly) geometries. During the operation procedures, the tracers travel in the drilling strings through the drill bit and return to the surface across the annulus. The data is then exported from the tracers for a quasi-real-time analysis. The results showed high success rates, four out of six microchips were successfully retrieved, and the data was made available for immediate analysis. This paper explains the challenges faced during the logging and interpretation of the data needed to define the wellbore characteristics for efficient drilling processes. The developed time-stepping algorithm correlates the measurement timestamp with the calculated depth. Lastly, the report summarizes the highlights of the tracers in terms of density, release mechanism, and collection method.
Naturally Presented MHC Ligands Carrying Glycans (2006)

Glycosylation is the most common and most diverse posttranslational modification of proteins. During tumorigenesis altered glycosylation patterns are a hallmark. In addition, changes in glycosylation are important in autoimmune diseases, such as rheumatoid arthritis. MHC class I- as well as MHC class II-presented glycopeptides have been described, and both CD8+ and CD4+ T cells are able to recognize glycopeptides in a classical MHC-restricted way. Different glycosylation patterns may influence T cell responses during disease, creating neoepitopes or blocking existing T cell responses to unmodified peptides by changing epitopes or abolishing binding to MHC molecules. In the present review, we discuss the current knowledge of glycosylated MHC class I and II peptides - which peptides have been described, and what is their impact on cellular immunity.
Cold Denaturation Unveiled: Molecular Mechanism of the Asymmetric Unfolding of Yeast Frataxin (2015)

What is the mechanism that determines the denaturation of proteins at low temperatures, which is, by now, recognized as a fundamental property of all proteins? We present experimental evidence that clarifies the role of specific interactions that favor the entrance of water into the hydrophobic core, a mechanism originally proposed by Privalov but never proved experimentally. By using a combination of molecular dynamics simulation, molecular biology, and biophysics, we identified a cluster of negatively charged residues that represents a preferential gate for the entrance of water molecules into the core. Even single-residue mutations in this cluster, from acidic to neutral residues, affect cold denaturation much more than heat denaturation, suppressing cold denaturation at temperatures above zero degrees. The molecular mechanism of the cold denaturation of yeast frataxin is intrinsically different from that of heat denaturation.
ChemInform Abstract: A Convenient Synthesis of Linear Pyridinoimidazo[1,2‐a]pyridine and Pyrroloimidazo[1,2‐a]pyridine Cores. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Ramucirumab in metastatic colorectal cancer: evidence to date and place in therapy (2016)

Colorectal cancer is the third most frequent cancer worldwide. Overall survival rates have improved greatly over the last few years due, at least in part, to the addition of targeted therapies to standard of care chemotherapy. Angiogenesis plays an important role in colorectal cancer, and therapies directed against the vascular endothelial growth factor (VEGF) axis have contributed significantly to improving the outcome of patients with metastatic colorectal cancer. Over the past few years, several new targeted antiangiogenic agents have been approved for this patient population, confirming the value of inhibiting tumour angiogenesis. The most recent among them is ramucirumab, a fully humanized monoclonal antibody that targets the extracellular domain of VEGF receptor 2. It has proven valuable in multiple tumour types including colorectal cancer. Several phase I and II clinical trials showed a favourable toxicity profile and promising clinical antitumour efficacy in colorectal cancer patients. In the phase III RAISE clinical trial, the addition of ramucirumab to FOLFIRI-based chemotherapy resulted in an improvement of overall survival in patients with metastatic colorectal cancer who had been previously treated with bevacizumab, oxaliplatin and a fluoropyrimidine. On the basis of these results, ramucirumab was approved by the US Food and Drug Administration for this setting. We present an overview of the key preclinical and clinical studies in the development of ramucirumab in the context of metastatic colorectal cancer.
Development of a New Prototype for an Educational Partnership in Nursing (2009)

The purpose of this 2-year study was to evaluate a new educational partnership for a nurse-managed community center in Taiwan. This project provided care to senior residents in an apartment complex and was integrated into a nursing educational program in Taiwan. In collaboration with these organizations, an educational partnership model was developed and evaluated. Three goals were accomplished: to provide health care to elderly residents, practical learning experience for nursing students, and practical experience for nursing faculty. The findings indicated that teachers, students, and residents in the apartment complex presented a high level of satisfaction with this model. The model created a vivid, practical, community-based resource and applied the principles of service-learning into practical nursing education in Taiwan. The model not only facilitated the empowerment of involved organizations, but also served as a reference for developing other community center-based nursing educational partnerships.
MicroProtein-Mediated Recruitment of CONSTANS into a TOPLESS Trimeric Complex Represses Flowering in Arabidopsis (2016)

MicroProteins are short, single domain proteins that act by sequestering larger, multi-domain proteins into non-functional complexes. MicroProteins have been identified in plants and animals, where they are mostly involved in the regulation of developmental processes. Here we show that two Arabidopsis thaliana microProteins, miP1a and miP1b, physically interact with CONSTANS (CO) a potent regulator of flowering time. The miP1a/b-type microProteins evolved in dicotyledonous plants and have an additional carboxy-terminal PF(V/L)FL motif. This motif enables miP1a/b microProteins to interact with TOPLESS/TOPLESS-RELATED (TPL/TPR) proteins. Interaction of CO with miP1a/b/TPL causes late flowering due to a failure in the induction of FLOWERING LOCUS T (FT) expression under inductive long day conditions. Both miP1a and miP1b are expressed in vascular tissue, where CO and FT are active. Genetically, miP1a/b act upstream of CO thus our findings unravel a novel layer of flowering time regulation via microProtein-inhibition.
<em>Pneumocystis</em> as a Co-Factor in Pulmonary Diseases (2018)

&lt;i&gt;Pneumocystis&lt;/i&gt; causes life-threatening pneumonia in immunocompromised populations. More recently it has been implicated as a co-factor in a number of chronic lung diseases including chronic obstructive pulmonary disease (COPD), severe asthma, and cystic fibrosis (CF). In this review, we will examine the current literature regarding &lt;i&gt;Pneumocystis&lt;/i&gt; and lung diseases in the HIV-infected patients and non-HIV immunocompromised populations, and the barriers to prophylaxis and treatment in these patients. Trimethoprim sulfamethoxazole (TMP-SMX) is an effective therapeutic against &lt;i&gt;Pneumocystis&lt;/i&gt; but this approach remains problematic due to drug interactions, treatment-limiting adverse events, and break-through &lt;i&gt;Pneumocystis&lt;/i&gt; pneumonia (PCP) despite prophylaxis. This review summarizes the shortcomings of current prophylaxis and treatment strategies, and the advances that have been made toward the development of novel diagnostics and therapeutics, with a focus on vaccine development.
A double-blind trial of oral immunotherapy for Artemisia pollen asthma with evaluation of bronchial response to the pollen allergen and serum-specific IgE antibody. (1990)

Eighteen asymptomatic Artemisia pollen asthma patients with normal pulmonary function were selected for a double-blind trial of oral immunotherapy. Each patient had a positive skin test to Artemisia pollen extract and also a positive bronchial challenge response to the same extract. The patients were randomly assigned to active treatment or placebo group and received intensive oral administration of Artemisia pollen extract over a 50-day course. The nine patients who received the active treatment ingested a cumulative dose of 396,652 PNU and showed a significant decrease in serum-specific IgE antibodies (P less than .05) and a significant reduction in bronchial sensitivity to the same extract (P less than .01). The changes in these two variables correlated well (r = .8787, P less than .01). The nine patients who received the placebo showed no significant changes in serum-specific IgE or bronchial sensitivity to Artemisia pollen extract. Follow-up of two cases with the same extract showed that the reductions in serum-specific IgE as well as bronchial sensitivity induced by oral immunotherapy were maintained for 3 months.
Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma (1997)

Human immunodeficiency virus type 1 (HIV-1) pol mutations are responsible for HIV-1 resistance to current antiretroviral drugs. HIV-1 RNA extraction with QIAamp HCV kit spin columns (Qiagen, Chatsworth, Calif.) followed by reverse transcription-PCR successfully recovered a 1,008-bp pol fragment from the plasma of 31 of 34 HIV-1-infected patients that was suitable for sequencing and recombinant-virus studies. The minimum HIV-1 RNA concentration required for gene recovery was 30 to 40 copies/ml, which was similar to the minimal HIV-1 RNA concentration required when phenol-chloroform or silica beads are used for RNA extraction.
Introduction of a Cys360Tyr Mutation in ANO5 Creates a Mouse Model for Gnathodiaphyseal Dysplasia (2020)

Gnathodiaphyseal dysplasia (GDD) is a rare autosomal dominant genetic disease characterized by the osteosclerosis of tubular bones and the formation of cemento-osseous lesions in mandibles. Although genetic mutations for GDD have been identified in the ANO5/TMEM16E gene, the cellular and molecular mechanisms behind the pathogenesis of GDD remain unclear. Here, we generated the first knock-in mouse model for GDD with the expression of human mutation p.Cys360Tyr in ANO5. Homozygous Ano5 knock-in mice (Ano5<sup>KI/KI</sup> ) replicated GDD-like skeletal features, including massive jawbones, bowing tibia, bone fragility, sclerosis, and cortical thickening of the femoral and tibial diaphysis. Serum alkaline phosphatase (ALP) levels were elevated in Ano5<sup>KI/KI</sup> mice as in GDD patients with p.Cys360Tyr mutation. Calvaria-derived Ano5<sup>KI/KI</sup> osteoblast cultures showed increased osteoblastogenesis, including hypermineralized bone matrix and enhanced bone formation-related factors expression. Interestingly, Ano5<sup>KI/KI</sup> bone marrow-derived macrophage cultures showed decreased osteoclastogenesis, and Ano5<sup>KI/KI</sup> osteoclasts exhibited disrupted actin ring formation, which may be associated with some signaling pathways. In conclusion, this new mouse model may facilitate elucidation of the pathogenesis of GDD and shed more light on its treatment. © 2021 American Society for Bone and Mineral Research (ASBMR).