gebhart/patrepeval huggingface dataset:
Transgenic GATA1ie3.9int - DAPK2 Mice Exhibit Low-Grade Anemia, Deficient Stress Erythropoiesis, and Compromised Erythroblast Survival. (2006)

Abstract Death-associated protein kinases (DAPKs) include DAPK-1, DAPK-2, ZIPK and death receptor associated kinase 1 &amp; 2 (DRAK-1 and -2). DAPK-1 and -2 are highly homologous in their shared serine-threonine kinase domains, and calmodulin and phospho-serine regulatory regions. DAPK1 is cytoskeletally associated via ankyrin repeats, and modulates a p19ARF/p53 pathway. DAPK2 is cytoplasmic, less widely distributed, and is expressed predominantly in erythroid cells. Hypothesized effects of DAPK2 on erythroblast formation presently were tested by preparing and studying several independent lines of GATA1ie3.9int-DAPK2 transgenic mice. At steady-state, hematocrits in GATA1ie3.9int-DAPK2 mice were mildly yet significantly decreased (while other lineages were essentially unaffected). Reticulocyte production also was modestly increased, and spleen morphologies were altered. During hemolytic anemia, however, DAPK2 mice exhibited comparably sharp deficiencies in stress erythropoiesis including prolonged decreases in hematocrits (as observed in repeated experiments at varied phenylhydrazine dosing). The basis for this stress-associated erythropoietic defect due to DAPK2 was investigated further in primary bone marrow erythroid progenitor cells ex vivo. A stage-specific defect in erythroblast formation was discovered specifically at KitnegCD71highTer119neg stage. Later stage CD71highTer119pos DAPK2-erythroblasts also were somewhat under-represented (but perhaps as a consequence of decreased KitnegCD71high pools.) In addition, DAPK2-progenitors gave rise to increased frequencies of Annexin V positive apoptotic erythroblasts. These first-time analyses of DAPK2 function in a relevant primary cell model reveal negative regulation of stage-specific erythroblast pool selectively during stress erythropoiesis, and in part via pro-apoptotic mechanisms.
Reperfusion injury, antioxidants and hemodynamics during orthotopic liver transplantation (1994)

Endothelial injury occurs as a result of oxygen free radical production after ischemia and reperfusion of transplanted livers, causing hemodynamic disturbance. Patients with chronic liver disease generally have low levels of fat-soluble vitamins, which have important antioxidant roles. We therefore assessed circulating levels of the antioxidants vitamin A, vitamin E, beta-carotene and lycopene, indices of lipid peroxidation and hemodynamic changes during elective orthotopic liver transplantation in 12 patients. We found that initial antioxidant levels were severely depleted compared with healthy subjects, and in some patients carotene and lycopene levels were undetectable. Increased lipid peroxidation was also evident, as shown by thiobarbituric acid-reactive substances. On reperfusion of the liver graft, vitamin A and E levels fell (p < 0.01) and were associated with decreases in systemic vascular resistance (p < 0.02). These data show that patients undergoing liver transplant have lowered antioxidant defenses and evidence of free radical damage, which compound the additional insult of reperfusion injury. Antioxidant therapy in these patients before transplantation may ameliorate the effects of reperfusion.
Aulas de física em escolas da rede pública de São Carlos, Brasil : desde uma visão dos alunos (2013)

O objetivo deste trabalho foi identificar e tecer considerações sobre estratégias no Ensino de Física que realmente são realizadas em sala de aula, para isto alunos e professores apresentaram as atividades que vivenciam no processo nas aulas de Física em escolas públicas da cidade de São Carlos. Obteve-se que nas aulas, em sua maioria, os alunos resolvem exercícios, participam com dúvidas e apontamentos e que os professores buscam uma contextualização da Física ao cotidiano dos alunos. Evidenciou-se uma carência quanto às aulas experimentais e ao uso das Tecnologias da Informação e Comunicação. Do analisado considera-se a necessidade de uma diversificação das aulas com as diferentes estratégias acima descritas o que poderia vir a despertar um maior interesse dos alunos pela Física.
Cyclic AMP‐dependent protein kinase phosphorylates group III metabotropic glutamate receptors and inhibits their function as presynaptic receptors (2001)

Recent evidence suggests that the functions of presynaptic metabotropic glutamate receptors (mGluRs) are tightly regulated by protein kinases. We previously reported that cAMP-dependent protein kinase (PKA) directly phosphorylates mGluR2 at a single serine residue (Ser843) on the C-terminal tail region of the receptor, and that phosphorylation of this site inhibits coupling of mGluR2 to GTP-binding proteins. This may be the mechanism by which the adenylyl cyclase activator forskolin inhibits presynaptic mGluR2 function at the medial perforant path-dentate gyrus synapse. We now report that PKA also directly phosphorylates several group III mGluRs (mGluR4a, mGluR7a, and mGluR8a), as well as mGluR3 at single conserved serine residues on their C-terminal tails. Furthermore, activation of PKA by forskolin inhibits group III mGluR-mediated responses at glutamatergic synapses in the hippocampus. Interestingly, beta-adrenergic receptor activation was found to mimic the inhibitory effect of forskolin on both group II and III mGluRs. These data suggest that a common PKA-dependent mechanism may be involved in regulating the function of multiple presynaptic group II and group III mGluRs. Such regulation is not limited to the pharmacological activation of adenylyl cyclase but can also be elicited by the stimulation of endogenous G(s)-coupled receptors, such as beta-adrenergic receptors.
The Future of Antibiotics and Resistance: A Tribute to a Career of Leadership by John Bartlett (2014)

The ways we have developed, used, and protected antibiotics have led, predictably, to our current crisis of rising antibiotic resistance and declining new treatments. If we want to stave off a postantibiotic era, we need to fundamentally change our approach. We need to challenge long-standing assumptions and cherished beliefs. We need to push through the reflexive resistance and excuses (eg, "that's not how we do things" and "that can't be done") that result from challenging established ways. Excuses abound. Action is needed. Ultimately, we need a coordinated national action plan to combat resistance. Herein we discuss 7 tasks and 3 common themes that cut across those tasks, which are necessary to achieve long-term success in dealing with antibiotics and resistance. These principles derive from many years of dialogue with Dr John Bartlett. The field of infectious diseases, and indeed medicine in general, has benefited immeasurably from his remarkable leadership.
Displaying Libraries of Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions (2003)

A number of systems have been developed over recent years that allow the selection of sequences with desired binding specificities from highly diverse, randomly generated peptlde libraries (1–3), When a member of a peptide library mteracts with a target blomolecule during one of these selections, both its primary sequence and its conformation play Important roles in the recognition process However, typically in these systems the peptides are tethered to one end of a fusion partner protein, where they possess many degrees of conformatlonal freedom. These flexible peptides may possess lower affinities for their target mole cules than the same peptldes presented as part of a folded native protein structure or in a more constrained cyclic structure (4–7). To circumvent this problem, various “protein scaffolds” have been proposed to help small peptides retain their conformations by restricting chain flexibility (8–10).
The aetiology of myocardial injury after non-cardiac surgery (2013)

Recognition of myocardial injury after non-cardiac surgery is difficult, since strong analgesics (e.g. opioids) can mask anginal symptoms, and ECG abnormalities are subtle or transient. Thorough knowledge of the pathophysiological mechanisms is therefore essential. These mechanisms can be subdivided into four groups: type I myocardial infraction (MI), type II MI, non-ischaemic cardiac pathology, and non-cardiac pathology. The incidence of type I MI in patients with a clinical suspicion of perioperative acute coronary syndrome (ACS) is 45-57 %. This percentage is higher in patients with a high likelihood of MI such as patients with ST-elevation ACS. Of note, the generalisability of this statement is limited due to significant study limitations. Non-ischaemic cardiac pathology and non-cardiac pathology should not be overlooked as a cause of perioperative myocardial injury (PMI). Especially pulmonary embolism and dysrhythmias are a common phenomenon, and may convey important prognostic value. Implementation of routine postoperative troponin assessment and accessible use of minimally invasive imaging should be considered to provide adequate individualised therapy. Also, addition of preoperative imaging may improve the stratification of high-risk patients who may benefit from preoperative or perioperative interventions.
Survival of Alpha and Intrinsically Photosensitive Retinal Ganglion Cells in NMDA-Induced Neurotoxicity and a Mouse Model of Normal Tension Glaucoma (2019)

These results suggest that αRGC and ipRGC are highly tolerant to NMDA-induced neurotoxicity and NTG-like neurodegeneration in GLAST KO mice.
Pervasive Service Composition in the Home Network (2007)

The home environment becomes ready to host distributed devices dynamically adapting to service availability and reacting to user location and user activity. Sensors, high definition rendering systems, home gateways, wired and wireless controllable equipments are now available. Many protocols enable connectivity and interaction between devices. However, challenges remain: protocol heterogeneity, interface fragmentation and device composition static aspect make self-organization and dynamic reconfiguration hardly achievable. This paper describes attractive scenarios at home which lead to the definition of the pervasive service composition requirements. A software architecture facing the mentioned challenges is proposed over OSGi. It first enables developers to implement distributed plug-n-play applications like a local one. It also delivers a service-oriented middleware allowing spontaneous distributed service composition to occur at runtime.
A Randomized Controlled Trial of Mindfulness-Based Stress Reduction for Women With Early-Stage Breast Cancer Receiving Radiotherapy (2013)

MBSR appears to facilitate psychosocial adjustment in BrCA patients receiving radiotherapy, suggesting applicability for MBSR as adjunctive therapy in oncological practice.
High-performance photoconductive terahertz sources based on nanoscale contact electrode gratings (2012)

A photoconductive terahertz emitter based on nanoscale contact electrode gratings is presented and experimentally demonstrated. The nanoscale grating enables ultrafast and high quantum efficiency device operation simultaneously, by reducing the photo-generated carrier transport path to photoconductor contact electrodes. The presented photoconductor eliminates the need for a short-carrier lifetime semiconductor, which limits the efficiency of conventional photoconductive terahertz emitters. A photoconductive terahertz emitter based on the presented scheme is implemented and integrated with 0.5 THz dipole antenna arrays. Emitted radiation is characterized in a terahertz time-domain spectroscopy setup, measuring a full width at half maximum response time of 670 fs.
Resonant Frequency Control of a Microspherical Cavity by Temperature Adjustment (2004)

We demonstrate frequency tuning of whispering-gallery mode resonances in a fused-silica microsphere by means of temperature control. Scattering spectra of the microsphere are measured using a tunable narrow-linewidth diode laser, and the frequency scale is precisely calibrated from a simultaneously observed spectrum of rubidium vapor. The resonant frequencies of whispering-gallery modes can be varied linearly by temperature elevation and lowering, at a rate of -2.6 GHz/K for a 190 µm sphere.
Thermotropic phase behavior of model membranes composed of phosphatidylcholines containing dl-methyl anteisobranched fatty acids. 1. Differential scanning calorimetric and phosphorus-31 NMR spectroscopic studies (1987)

The thermotropic phase behavior of aqueous dispersions of nine dl-methyl branched anteisoacylphosphatidylcholines was studied by differential scanning calorimetry and 31P nuclear magnetic resonance spectroscopy. The calorimetric studies demonstrate that these compounds all exhibit a complex phase behavior, consisting of at least two minor, low-enthalpy, gel-state transitions which occur at temperatures just prior to the onset of the gel/liquid-crystalline phase transition. In addition, at still lower temperatures, anteisobranched phosphatidylcholines containing fatty acyl chains with an odd number of carbon atoms show a major, higher enthalpy, gel-state transition, which was assigned to a conversion from a condensed to a more loosely packed gel phase. No such transition was observed for the even-numbered compounds in aqueous dispersion, but when dispersed in aqueous ethylene glycol, a major gel-state transition is clearly discernible for two of the even-numbered phospholipids. The major gel-state transition exhibits heating and cooling hysteresis and is fairly sensitive to the composition of the bulk aqueous phase. 31P NMR spectroscopic studies indicate that the major gel-state transition is accompanied by a considerable change in the mobility of the phosphate head group and that, at temperatures just prior to the onset of the gel/liquid-crystalline phase transition, the mobility of the phosphate head group is comparable to that normally exhibited by the liquid-crystalline state of most other phospholipids. The temperatures at which the gel/liquid-crystalline phase transition occurs and the enthalpy change associated with this process are considerably lower than those of the saturated n-acyl-PC's of comparable acyl chain length.(ABSTRACT TRUNCATED AT 250 WORDS)
Longitudinal study of visual functions in young insulin dependent diabetics (1994)

The aim of this study was to compare the visual functions of a group of young insulin dependent diabetes mellitus patients (IDDMs), (n= 42) with an age and sex matched control group (n= 24). Examinations were carried out every 3 months for 2 years. There were no significant differences in visual acuity between IDDMs and control subjects. The IDDMs made significantly more errors with the desaturated D15 compared with the control group (P < 0.05). The contrast sensitivity for the IDDMs was lower at each spatial frequency compared with the control group, being significantly different from 3 c/deg and above (P < 0.05). A negative correlation was found between HbA1, and contrast sensitivity at 6 and 12 c/deg; as the HbA1 increased the contrast sensitivity decreased (P < 0.05). As the blood glucose level decreased, the colour vision deteriorated (P < 0.05). There was no significant difference between the visual performance of those IDDMs with retinopathy (n= 5) compared to those without.
In-system phase measurement of pulsed microwave amplifiers (2004)

This paper discusses measurement techniques that can estimate the phase of pulsed microwave amplifiers. In addition to the traditional techniques that use a phase bridge or vector network analyzer, a dual channel downconverter (DCD) technique was developed. The use of this patent-pending DCD unit offers ease of installation, complex stimulus modulation compatibility, low-cost, supportability, stability, and accuracy.
Mir-190b negatively contributes to the Trypanosoma cruzi- infected cell survival by repressing PTEN protein expression (2015)

Chagas disease, which is caused by the intracellular protozoan Trypanosoma cruzi, is a serious health problem in Latin America. The heart is one of the major organs affected by this parasitic infection. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection, and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. Previous studies have reported that the establishment of parasitism is connected to the activation of the phosphatidylinositol-3 kinase (PI3K), which controls important steps in cellular metabolism by regulating the production of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Particularly, the tumour suppressor PTEN is a negative regulator of PI3K signalling. However, mechanistic details of the modulatory activity of PTEN on Chagas disease have not been elucidated. To address this question, H9c2 cells were infected with T. cruzi Berenice 62 strain and the expression of a specific set of microRNAs (miRNAs) were investigated. Our cellular model demonstrated that miRNA-190b is correlated to the decrease of cellular viability rates by negatively modulating PTEN protein expression in T. cruzi-infected cells.
CpG Island Methylator Phenotype in Colorectal Cancers: Comparison of the New and Classic CpG Island Methylator Phenotype Marker Panels (2008)

Abstract Context. —CpG island methylator phenotype (CIMP) designates a subset of colorectal cancers featuring concordant hypermethylation of multiple promoter CpG islands. Little is known about the clinical outcome or histologic characteristics of CIMP-positive colorectal cancers defined by recently identified CpG island methylator phenotype panels. Objective. —To investigate and compare the molecular and clinicopathologic features of CIMP-positive colorectal cancers defined by classic (p16, hMLH1, MINT1, MINT2, MINT31) and new (CACNA1G, IGF2, NEUROG1, RUNX3, SOCS1) CIMP panels. Design. —We analyzed 130 colorectal cancers for hypermethylation of both panels using methylation-specific polymerase chain reaction. Results. —With at least 2 markers methylated, both classic (39/130; 23.1%) and new (23.1%) CIMP-positive colorectal cancers were significantly associated with proximal tumor location, microsatellite instability, and BRAF mutation (all P values were less than .05). The new panel outperformed the classic panel in detecting these features. With at least 3 markers methylated, new CIMP-positive colorectal cancers (16.9%) were closely associated with proximal tumor location, low frequency of KRAS mutation, and high frequency of BRAF mutation (all P values were less than .05), whereas classic CIMP-positive colorectal cancers (18.5%) were closely associated with proximal tumor location, frequent microsatellite instability, and frequent BRAF mutation (all P values were less than .05). Analyzing a combination of CIMP and microsatellite instability status, CIMP-positive/microsatellite instability–negative colorectal cancers had the worst clinical outcomes. Conclusions. —Whereas the classic panel outperformed in predicting clinical outcome, the new panel was superior in detecting known clinicopathologic features of CIMP but inferior in prognostication power.
Preparation of Hierarchically Porous Nickel from Macroporous Silicon (2006)

A novel approach for preparing hierarchical porous nickel structures has been developed. It is an aqueous processing of the template, macroporous silicon, which was etched in HF solution to have straight channels. By immersion in a prepared nickel solution, the silicon sidewalls were replaced by nickel deposits to form a total metallic structure. Since silicon framework was gradually consumed during the displacement reactions, the deposited nickel displayed additional feature of being highly porous on the sidewalls. With the initial straight microchannels, the metal structure was hierarchically porous.
Analysis of 5G New Radio Uplink Signals on an Analogue-RoF System Based on DSP-Assisted Channel Aggregation (2018)

The 3rd Generation Partnership Project (3GPP) is in the process of developing 5th generation (5G) radio access technology, the so-called new radio (NR). The aim is to achieve the performance requirements forIMT-2020 radio interface technology. In this paper, we focus on the analysis of the transmission of 5G NR uplink physical channels, such as physical uplink shared channel (PUSCH) and physical uplink control channel (PUCCH), dedicated for data and control channels, respectively, as specified in the 3GPP standard, using digital signal processing (DSP)-assisted frequency division multiple access (FDMA) and time division multiple access (TDMA) channel aggregation techniques on an analogue radio-over-fiber (A-RoF) architecture. We verified that there is ~34% spectral efficiency gain and lower error vector magnitude (EVM) achieved using the TDMA technique.
Response of different genotypes of Brassica carinata to microspore culture (1999)

Abstract Sixteen lines of Brassica carinata were evaluated for microspore culture and plant regeneration. The highest values of cell division and embryo yield resulted from buds between 2.5 and 3.5 mm long with 3 days of pretreatment at 32°C and plating densities of 100 000‐150 000 microspores/ml. Ten out of 16 lines tested (63%) responded positively to microspore culture. In all breeding and F 1 lines, both cell division and embryo yield varied over a wide range, but embryogenic response was higher in F 1 lines than in the breeding lines.
Transformation of a set of slices rotated on a common axis to a set of Z-slices: Application to three-dimensional visualization of the in vivo human lens (1997)

A technique that transforms a set of images acquired as a rotating frame about an axis (Z axis) into a set of images along the Z axis in presented. This technique is applied to the three-dimensional visualization of the in vivo human lens. A Scheimpfling slit camera acquired 60 optical images through the in vivo human lens. Between each image acquisition the plane containing the slit beam of light was sequentially rotated. This set of 60 images was transformed into a new stack of images on the Z axis. The transformed stack of Z images was visualized with volume rendering software.
AT9283, a novel aurora kinase inhibitor, suppresses tumor growth in aggressive B‐cell lymphomas (2011)

Aurora kinases are oncogenic serine/threonine kinases that play key roles in regulating the mitotic phase of the eukaryotic cell cycle. Auroras are overexpressed in numerous tumors including B-cell non-Hodgkin's lymphomas and are validated oncology targets. AT9283, a pan-aurora inhibitor inhibited growth and survival of multiple solid tumors in vitro and in vivo. In this study, we demonstrated that AT9283 had potent activity against Aurora B in a variety of aggressive B-(non-Hodgkin lymphoma) B-NHL cell lines. Cells treated with AT9283 exhibited endoreduplication confirming the mechanism of action of an Aurora B inhibitor. Also, treatment of B-NHL cell lines with AT9283 induced apoptosis in a dose and time dependent manner and inhibited cell proliferation with an IC(50) < 1 μM. It is well known that inhibition of auroras (A or B) synergistically enhances the effects of microtubule targeting agents such as taxanes and vinca alkaloids to induce antiproliferation and apoptosis. We evaluated whether AT9283 in combination with docetaxel is more efficient in inducing apoptosis than AT9283 or docetaxel alone. At very low doses (5 nM) apoptosis was doubled in the combination (23%) compared to AT9283 or docetaxel alone (10%). A mouse xenograft model of mantle cell lymphoma demonstrated that AT9283 at 15 mg/kg and docetaxel (10 mg/kg) alone had modest anti-tumor activity. However, AT9283 at 20 mg/kg and AT9283 (15 or 20 mg/kg) plus docetaxel (10 mg/kg) demonstrated a statistically significant tumor growth inhibition and enhanced survival. Together, our results suggest that AT9283 plus docetaxel may represent a novel therapeutic strategy in B-cell NHL and warrant early phase clinical trial evaluation.