gebhart/patrepeval huggingface dataset:
Sugar-Assisted Ligation in Glycoprotein Synthesis (2007)

Sugar-assisted ligation (SAL) presents an attractive strategy for the synthesis of glycopeptides, including the synthesis of cysteine-free beta-O-linked and N-linked glycopeptides. Here we extended the utility of SAL for the synthesis of alpha-O-linked glycopeptides and glycoproteins. In order to explore SAL in the context of glycoprotein synthesis, we developed a new chemical synthetic route for the alpha-O-linked glycoprotein diptericin epsilon. In the first stage of our synthesis, diptericin segment Cys(Acm)37-Gly(52) and segment Val(53)-Phe(82) were assembled by SAL through a Gly-Val ligation junction. Subsequently, after Acm deprotection, diptericin segment Cys(37)-Phe(82) was ligated to segment Asp(1)-Asn(36) by means of native chemical ligation (NCL) to give the full sequence of diptericin epsilon. In the final synthetic step, hydrogenolysis was applied to remove the thiol handle from the sugar moiety with the concomitant conversion of mutated Cys(37) into the native alanine residue. In addition, we extended the applicability of SAL to the synthesis of glycopeptides containing cysteine residues by carrying out selective desulfurization of the sulfhydryl-modified sugar moiety in the presence of acetamidomethyl (Acm) protected cysteine residues. The results presented here demonstrated for the first time that SAL could be a general and useful tool in the chemical synthesis of glycoproteins.
Interactions between melatonin and prolactin during gestation in mink ( Mustela vison ) (1990)

Immature female mink, 8 weeks of age in July, were treated with implants releasing melatonin. Mating, which induced ovulation, took place during the normal breeding season in the following March. Circulating prolactin and progesterone concentrations did not undergo the expected gestational increases, and no embryos implanted. A similar absence of gestational changes in prolactin and progesterone values ensued in primiparous mink treated with the melatonin implant 2-3 days after the second of 2 matings. Administration of exogenous sheep prolactin (0.5 mg/day) by minipump induced precocious elevation of progesterone concentrations in mated mink. Prolactin administration overcame the effects of melatonin, in that the corpora lutea were activated and embryos implanted, but exogenous prolactin resulted in degeneration of implanted embryos both in the presence and absence of chronic melatonin. The results suggest that melatonin has a single effect in alteration of gestation in mink; i.e. the prevention of prolactin secretion. Hyperprolactinaemia may inhibit embryo development in this species.
Développement de microsystèmes EWOD sur surfaces hydrophobes et superhydrophobes - Application à la spectrométrie de masse (2007)

Thèse de doctorat en Microondes et Microtechnologies, Université de Lille 1, 2 octobre
Digital rights (and wrongs) (2002)

New legislation intended to resolve intellectual property issues only puts opposing viewpoints in stark relief
Structure-function relationships of epidermal growth factor modules in vitamin K-dependent clotting factors (1991)

ODULES* homologous to the epidermal growth M factor (EGF) precursor have been found in a large number of proteins with diverse functions.Among these proteins are the vitamin K-dependent clotting factors VII, IX, and X, proteins C and S of the protein C anticoagulant system, and the nonvitamin-K-dependent proteins urokinase (uPA), tissue-type plasminogen activator (tPA), and factor XI1 (Fig l).1.7Progress toward an understanding of structure-function relationships of EGF modules in vitamin K-dependent proteins has advanced along four lines of inquiry.First, the application of high-resolution NMR spectroscopy to structural studies has shown a striking similarity between EGF,"" transforming growth factor-a (TGR-cx),'~"~'~ and the NH,-terminal EGF module of factor X.I3 Second, the properties of factor IX molecules with mutations in the NH,-terminal EGF module have provided insight into the function of these module^.'^.'^Third, EGF module-containing proteolytic fragments of factors IX1* and X 1 9 3 ' 0 and protein Cz1,22 and protein S,U and a recombinant EGF module of factor IX,24 have been shown to bind Ca2+, thus defining a new class of metal ion-binding sites that is presumably crucial to many fundamental biologic p r o c e ~s e s .' ~, ~' ~~~ Fourth, fragments that contain such EGF modules either free or linked to the y-carboxyglutamic acid-containing region are being used to study inter-module interaction^.^^-^'The purpose of this review is to give an account of recent findings regarding the structure and function of EGF modules in vitamin K-dependent plasma proteins and relate the results to advances in the understanding of the function of similar modules in other proteins.For information about the reactions of the vitamin K-dependent enzyme complexes, the reader is referred to a review recently published in this journal.'*There have been several reviews on EGF*8*Z9,30 and EGF modules in nonvitamin K-dependent *The term module is used throughout this report as suggested by Patthy',' and by Baron et al, ' rather than domain, repeat, or motif.However, in accordance with the commonly used nomenclature, vitamin K-dependent proteins lacking the y-carboxyglutamic acidcontaining module will be referred to as, eg, Gla-domainless factor X rather than Gla-moduleless factor X.
Dataset of breakthrough time for various modified sand materials using Rhodamine-B as an adsorbate (2019)

Removal of synthetic dyes from wastewater generated by the textile industries is important. Rhodamine-B is widely used colorant and is medically proven to lead to tissue borne sarcoma, reproductive and neurotoxicity issues in humans, if still present in the treated drinking water. Herein, this dataset provides information on different forms of sand materials for their effective utilization as an adsorbent material for Rhodamine-B. The effectiveness of the media was measured in terms of breakthrough time obtained. One of the 27 presented data set is a part of a research article [1] explaining the breakthrough time of these filter media under specific experimental condition. All these data is a combination of three variables that were studied: a) concentration of Rhodamine-B (1 mg/L, 5 mg/L and 10 mg/L), b) flow velocity of Rhodamine-B spiked water (2 mL/min, 5 mL/min and 10 mL/min) and c) bed height (7.5 cm, 10 cm, and 12.5 cm). At any bed height, the breakthrough time of graphitized sand (brewery sugar coated, GS1) was found to be 3-4 times higher than the second best adsorbent, i.e., manganese dioxide coated on GS1.
Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation (2003)

Zebrafish with the young (yng) mutation show a defect in retinal cell differentiation. Here we demonstrate that a mutation in a brahma-related gene (brg1) is responsible for the yng phenotype. Brahma homologues function as essential subunits for SWI/SNF-type chromatin remodeling complexes. Our analysis indicates that brg1 is required for the wave of mitogen-activated protein kinase activity that precedes retinal cell differentiation. Using specific inhibitors of the mitogen-activated protein kinase pathway we show this signal has a direct role in retinal cell differentiation. Lastly, through investigations of mutants in other chromatin remodeling subunits, we provide genetic evidence for gene and tissue specificity of the Brahma chromatin remodeling complex.
Behaviour of laser induced plasma, keyhole and reflected light during laser welding with superimposed beams of different wavelengths (2003)

This paper deals with the behavior of a keyhole and a laser-induced plasma observed during laser welding of several metals with a single YAG laser or superimposed two beams through the ultra high-speed video camera, with the objective of understanding enhanced beam absorption due to two different wavelength laser beams. Moreover, reflected YAG laser beams were measured by photodiodes, and the relationship between the keyhole behavior and the reflected-laser photodiode signal was investigated to clarify rapid welding phenomena. It was observed that the behavior of a keyhole and a laser-induced plasma during laser welding was considerably different between a single beam and combined two different beams. And the reflected light intensity during welding with the superimposed two different laser beams lowered in the earlier stage than that with a single pulsed YAG laser beam.
Black Social Workers in Private Practice: (1991)

As social work moves into the 1990s the profession is finally beginning to reap some of the seeds sewn in the affirmative action programs of the 1960s and 70s. An increasing number of seasoned Black clinical social workers have successfully honed their practice skills in human service agencies and are venturing into the realm of private clinical practice. This paper explores those particular dilemmas Black private practitioners must face which differ substantially from their majority counterparts.
Detailed Mechanical Design Of A Large Diameter High Stability Centrifuge (1987)

A detailed mechanical design of a large diameter (120-inch radius) centrifuge was completed. The overall g-stability of the system under all operating conditions is projected to be 5 ppm. The design includes an externally pressurized dual gas bearing main axis spindle and a two-degree of freedom platform. Significant design features and methods are discussed.
Syndromic surveillance: STL for modeling, visualizing, and monitoring disease counts (2009)

The STL decomposition method for chief complaint counts leads to a rapid and accurate detection method for disease outbreaks, and requires only 90 days of historical data to be put into operation. The visualization tools that accompany the decomposition and outbreak methods provide much insight into patterns in the data, which is useful for surveillance operations.
Color-Coded Front-of-Pack Nutrition Labels—An Option for US Packaged Foods? (2020)

The implementation of a standardized front-of-pack-labelling (FoPL) scheme would likely be a useful tool for many consumers trying to improve the healthfulness of their diets. Our objective was to examine what the traffic light labelling scheme would look like if implemented in the US. Data were extracted from Label Insight’s Open Access branded food database in 2017. Nutrient levels and the proportion of products classified as “Red” (High), “Amber” (Medium) or “Green” (Low) in total fat, saturated fat, total sugar and sodium for food and beverage items were examined. The proportion of products in each category that had each possible combination of traffic light colors, and met the aggregate score for “healthy” was examined. Out of 175,198 products, >50% of all US packaged foods received a “Red” rating for total sugar and sodium. “Confectionery” had the highest mean total sugar (51.9 g/100 g) and “Meat and meat alternatives” the highest mean sodium (781 mg/100 g). The most common traffic light label combination was “Red” for total fat, saturated fat and sodium and “Green” for sugar. Only 30.1% of products were considered “healthy”. A wide variety (n = 80) of traffic light color combinations were observed. A color coded traffic light scheme appears to be an option for implementation across the US packaged food supply to support consumers in making healthier food choices.
Animal models (1994)

Different models of experimental autoimmune encephalomyelitis (EAE) have been successfully applied to investigate and manifold aspects of the autoimmune pathogenesis of multiple sclerosis. Studies using myelin-specific T-cell lines that transfer EAE to naive recipient animals established that only activated lymphocytes are able to cross the endothelial blood-brain barrier and cause autoimmune disease within the local parenchyma. All encephalitogenic T cells are CD4+ Th1-type lymphocytes that recognize autoantigenic peptides in the context of MHC class II molecules. In the case of myelin basic protein (MBP) specific EAE in the Lewis rat, the T-cell response is directed against one strongly dominant peptide epitope. The encephalitogenic T cells preferentially use one particular set of T-cell receptor genes. Although MBP is a strong encephalitogen in many species, a number of other brain protein are now known to induce EAE. These include mainly myelin components (PLP, MAG, and MOG), but also, the astroglial S-100 beta protein. Encephalitogenic T cells produce only inflammatory changes in the central nervous system, without extensive primary demyelination. Destruction of myelin and oligodendrocytes in these models requires additional effector mechanisms such as auto-antibodies binding to myelin surface antigens such as the myelin-oligodendrocyte glycoprotein.
Stable Amplified DNA in Drug-Resistant Leishmania Exists as Extrachromosomal Circles (1986)

The relative stability of amplified DNA in drug-resistant Leishmania major was previously reported to be dependent on location, that is, unstable amplified DNA was extrachromosomal and stable amplified DNA was chromosomal. Leishmanial chromosomes have now been directly examined by means of orthogonal-field-alternation gel electrophoresis (OFAGE). The amplified DNA's in three resistant cell lines displayed unusual migration and were clearly extrachromosomal, regardless of whether the amplified DNA's were stable or unstable. Thus, contrary to conclusions from earlier studies of drug resistance in cultured animal cells, stable amplified DNA in Leishmania can be extrachromosomal. In addition, these amplified DNA's were shown to be circular on the basis of their resistance to exonuclease III digestion and their behavior on OFAGE. Their mobility was also greatly changed after treatment with topoisomerase II, suggesting that the amplified DNA's were either supercoiled or concatenated circles.
Developing cell therapies as drug products (2020)

In the last 20 years, the global regulatory frameworks for drug assessment have been managing the challenges posed by using cellular products as new therapeutic tools. Currently, they are defined as "Advanced Therapy Medicinal Products", comprising a large group of cellular types that either alone or in combination with gene and tissue engineering technology. They have the potential to change the natural course of still lethal or highly debilitating diseases, including cancers, opportunistic infections and chronic inflammatory conditions. Globally, more than 50 cell-based products have obtained market authorization. This overview describes the advantages and unsolved challenges on developing cells as innovative therapeutic vehicles. The main cell therapy players and the legal framework are discussed, starting from chimeric antigen receptor T-cells for leukaemia and solid tumours, dealing then with lymphocytes as potent anti-microbiological tools and then focusing on mesenchymal stem/stromal cells whose role covers regenerative medicine, immunology and anti-tumour therapy.
Association of single-nucleotide polymorphisms in DNA repair genes with progression and survival of primary cutaneous melanoma patients (2008)

9018 Background: Host genetics has so far not been appreciated as an important factor in adjuvant and palliative study design in melanoma. Single nucleotide polymorphisms (SNPs) in DNA repair genes may affect not only cancer predisposition, but also progression and survival of primary cutaneous melanoma patients. We evaluated the association of SNPs in DNA repair genes with overall survival (OS), metastasis-free survival (MFS) and survival following the first metastasis (SFM) in patients with primarily unmetastasized melanoma. Methods: 13 SNPs in 8 DNA repair genes were evaluated in 400 primary melanoma patients (any T,N0,M0) using genotyping assays and associated with follow-up data of these patients. Deviation of Hardy-Weinberg equilibrium using the Pearson-x2 test, censored survivorship function Kaplan-Meier, log-rank test were used. Cox Hazards regression modeling and multivariate analysis models including age, gender and Breslow were employed. Results: Of the evaluated SNPs 4 showed significant associations with overall survival. Two of each were correlated with MFS but not with SFM and viceversa. For XRCC1 R399Q AA, the hazard ratio (HR) for MFS was 0.4 (95% CI, 0.21 to 0.78) and the HR for OS was 0.3 (95% CI, 0.11 to 0.90), while SFM was unchanged. Similarly, for ERCC2 K751Q CC the HR for MFS was 0.6 (95% CI, 0.35 to 0.94) and the HR for OS was 0.4 (0.21 to 0.93), while SFM was unchanged. MFS was unchanged for ERCC2 D312N AA, while the HR for SFM was 0.3 (95% CI, 0.10 to 0.78) and the HR for OS was 0.2 (95% CI, 0.09 to 0.66). For the -1843 XRCC3 promoter variant genotypes the HR for SFM was 1.9 (95% CI, 1.25 to 3.02) and the HR for OS was 1.6 (1.02 to 2.41), and MFS was also unaffected. Conclusions: SNPs in DNA repair genes may be differentially predictive of metastatic capacity and survival following metastasis in primary cutaneous melanoma patients. Impact of host genetics on clinical trial outcome needs to be systematically addressed. No significant financial relationships to disclose.
A Regioselective Approach to 5-Substituted-3-amino-1,2,4-triazines (2003)

Nucleophilic displacement of readily available alpha,alpha-dibromoketones with excess morpholine gave the corresponding ketoaminals, which upon condensation with aminoguanidine in MeOH in the presence of AcOH afforded 5-substituted-3-amino-1,2,4-triazines in >95% regioselectivity and 45-76% isolated yield. [reaction: see text]
Analysis of the Weld Seam Morphology of Polypropylene in Laser Transmission Welding (2018)

Laser transmission welding is a well-known joining technology for welding thermoplastics. Although the process is already used industrially, fundamental process-structure-property relationships are not fully understood and are therefore the subject of current research. One aspect of these mentioned process-structure-property relationships is the interaction between the temperature field during the welding process, the weld seam morphology of semi-crystalline thermoplastics, and the weld seam strength. In this study, the influence of the line energy on the weld seam morphology of polypropylenes is analyzed. For this purpose, the size of spherulites in the weld seam is investigated, as well as different occurring phases of polypropylene (α- and β-phase). It is shown that both the spherulite size of the α-phase and the amount of β-phase increase with increasing line energy. For the explanation and discussion of the results, a temperature-dependent thermal simulation model is used to derive characteristic attributes of the temperature field (maximum temperatures, cooling rates, temperature gradients).
Two-Step Process Identification With Correlation Analysis and Least-Squares Parameter Estimation (1974)

An identification method is described which first identifies a linear nonparametric model (crosscorrelation function, impulse response) by correlation analysis and then estimates the parameters of a parametric model (discrete transfer function) and also includes a method for the detection of the model order and the time delay. The performance, the computational expense and the overall reliability of this method is compared with five other identification methods. This two-step identification method, which can be applied off-line or on-line, is especially suited to identification by process computers, since it has the properties: Little a priori knowledge about the structure of the process model; very short computation time; small computer storage; no initial values of matrices and parameters are necessary and no divergence is possible for the on-line version. Results of an on-line identification of an industrial process with a process computer are shown.
Neutral core oligosaccharides of bovine submaxillary mucin (1998)

Oligosaccharide alditols were released from bovine submaxillary mucin by alkaline borohydride treatment. The fractions containing smaller neutral oligosaccharides were separated by HPLC, to give, in the mono-, di- and trisaccharide-alditol sizes, 19 different structures, in addition to three fucosylated tetrasaccharide alditols. Molecules were identified by NMR spectroscopy, electrospray-mass-spectrometry-mass-spectrometry, permethylation analyses, and highly selective Pb(OAc)4 oxidation at -30 degrees C, followed by borohydride reduction. Pb(OAc)4 oxidation was found to be generally applicable in identifying the branch position(s) of substitutions for all core structures. Among the isolated oligosaccharide alditols were structures not previously reported, including 12 structures not reported from bovine submaxillary mucin, and four structures (three core structures) not found in the Carbbank database.
Synthesis of new 1,3-thiazines by a three-compoment reaction of thiobenzamide with aromatic aldehydes and alkenes, under microwaves activation (2011)

National audience
Predictive Driving: Fusing Prediction and Planning for Automated Highway Driving (2019)

To guide an automated vehicle safely through complex traffic, knowledge about the future evolution of the driving situation has to be considered. The contribution at hand proposes an approach for automated driving in structured environments. An environment representation for trajectory planning is presented that enables predictive driving by interconnecting trajectory prediction for the surrounding traffic and planning of an according ego trajectory. A maneuver-based approach with an efficient trajectory model is carried out to enable an accurate and fast estimation of future motions of other vehicles. The results are directly considered in the dynamic environment representation utilized for trajectory planning. In the trajectory optimization process, the required safety distance is hence taken into account uniquely for each future time instance. The evaluation of the trajectory prediction approach shows very good performance on a simulated as well as on a dataset recorded by a test vehicle. On account of the predictive character of the developed environment potential field, simulation experiments demonstrate the feasibility and effectiveness of the proposed method.