gebhart/patrepeval huggingface dataset:
Localization of a putative magnesium‐binding site within the cytoplasmic domain of the sarcoplasmic reticulum Ca2+‐ATPase (1993)

The amino acid sequences of several P-type ATPases share regions of high similarity. The functions of some of these regions, although several proposals have been made, have not yet been absolutely identified. In particular, one of these domains, located within the cytoplasmic loop in the area known as the 'hinge' domain, exhibits the highest degree of conservation. In the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA-1), this region is located at residues 700-712. Comparison of the sequence in this domain with calcium-binding proteins reveals similarities with the center of the helix-loop-helix EF-hand structure that is known to form divalent-cation-binding sites. A 38-residue polypeptide, corresponding to the domain 682-719 of the Ca(2+)-ATPase was synthesized and tested for its ability to bind divalent cations. Circular-dichroism, intrinsic-fluorescence and fluorescence-energy-transfer studies performed on this polypeptide in solution support the hypothesis that this domain has, in the protein, the ability to bind a divalent cation, presumably Mg2+, with an affinity of 10-15 mM. This property is observed for the isolated polypeptide in aqueous solvent and in the presence of low concentrations of the alpha-helix promoter 2,2,2-trifluoroethanol. Substitution of either one or two critical amino acids in the sequence induces a significant reduction of the binding properties. It is proposed that this sequence is involved in the co-ordination of a Mg2+ in the nucleotide-binding site and/or in the phosphorylation site of P-type ATPases.
Detection of Bacterial Metabolic Volatile Indole Using a Graphene-Based Field-Effect Transistor Biosensor (2021)

The existence of bacteria is a great threat to food safety. Volatile compounds secreted by bacteria during their metabolic process can be dissected to evaluate bacterial contamination. Indole, as a major volatile molecule released by <i>Escherichia coli</i> (<i>E. coli</i>), was chosen to examine the presence of <i>E. coli</i> in this research. In this work, a graphene field-effect transistor (G-FET) was employed to detect the volatile molecule-indole based on a π-π stacking interaction between the indole and the graphene. The exposure of G-FET devices to the indole provokes a change in electrical signal, which is ascribed to the adsorption of the indole molecule onto the graphene surface via π-π stacking. The adsorption of the indole causes a charge rearrangement of the graphene-indole complex, which leads to changes in the electrical signal of G-FET biosensors with a different indole concentration. Currently, the indole biosensor can detect indole from 10 ppb to 250 ppb and reach a limit of detection of 10 ppb for indole solution detection. We believe that our detection strategy for detecting bacterial metabolic gas molecules will pave a way to developing an effective platform for bacteria detection in food safety monitoring.
Comparison of Intramedullary Nailing to Plating for Both-Bone Forearm Fractures in Older Children (2008)

Therapeutic level III--retrospective comparative study.
A computational approach for obstruction-free photography (2015)

We present a unified computational approach for taking photos through reflecting or occluding elements such as windows and fences. Rather than capturing a single image, we instruct the user to take a short image sequence while slightly moving the camera. Differences that often exist in the relative position of the background and the obstructing elements from the camera allow us to separate them based on their motions, and to recover the desired background scene as if the visual obstructions were not there. We show results on controlled experiments and many real and practical scenarios, including shooting through reflections, fences, and raindrop-covered windows.
Contrasts between patients with affective disorders and patients with schizophrenia on a neuropsychological test battery (1993)

These results suggest that patients with schizophrenia perform systematically worse on cognitive measures than patients with affective disorders, which is consistent with their generally poorer outcome. The results also indicate that schizophrenia and affective disorders are qualitatively distinguishable in neuropsychological terms, given differences in apparent intellectual deterioration, profiles of cognitive impairment, and associations between cognitive performance and psychopathology.
Effects of Respiratory Rate and Tidal Volume on Gas Exchange in Total Liquid Ventilation (2009)

Using a rabbit model of total liquid ventilation (TLV), and in a corresponding theoretical model, we compared nine tidal volume-respiratory rate combinations to identify a ventilator strategy to maximize gas exchange, while avoiding choked flow, during TLV. Nine different ventilation strategies were tested in each animal (n = 12): low [LR = 2.5 breath/min (bpm)], medium (MR = 5 bpm), or high (HR = 7.5 bpm) respiratory rates were combined with a low (LV = 10 ml/kg), medium (MV = 15 ml/kg), or high (HV = 20 ml/kg) tidal volumes. Blood gases and partial pressures, perfluorocarbon gas content, and airway pressures were measured for each combination. Choked flow occurred in all high respiratory rate-high volume animals, 71% of high respiratory rate-medium volume (HRMV) animals, and 50% of medium respiratory rate-high volume (MRHV) animals but in no other combinations. Medium respiratory rate-medium volume (MRMV) resulted in the highest gas exchange of the combinations that did not induce choke. The HRMV and MRHV animals that did not choke had similar or higher gas exchange than MRMV. The theory predicted this behavior, along with spatial and temporal variations in alveolar gas partial pressures. Of the combinations that did not induce choked flow, MRMV provided the highest gas exchange. Alveolar gas transport is diffusion dominated and rapid during gas ventilation but is convection dominated and slow during TLV. Consequently, the usual alveolar gas equation is not applicable for TLV.
Distributed mode filtering rod fiber amplifier delivering 292W with improved mode stability (2012)

We demonstrate a high power fiber (85 μm core) amplifier delivering up to 292 Watts of average output power using a mode-locked 30 ps source at 1032 nm. Utilizing a single mode distributed mode filter bandgap rod fiber, we demonstrate 44% power improvement before the threshold-like onset of mode instabilities by operating the rod fiber in a leaky waveguide regime. We investigate the guiding dynamics of the rod fiber and report a distinct bandgap blue-shifting as function of increased signal power level. Furthermore, we theoretically analyze the guiding dynamics of the DMF rod fiber and explain the bandgap blue-shifting with thermally induced refractive index change of the refractive index profile.
Mechanism of Carbon Absorption by Iron During Sintering of Iron—Graphite (1984)

AbstractIt has been shown previously that the existing theories on the mode of carbon transfer during sintering of iron-graphite are inadequate to explain the results obtained. From the literature, it is seen that large amounts of oxygen are adsorbed on graphite. Graphite was vacuum degassed at high temperature and sintering experiments using iron-degassed graphite were carried out at 1040°C in hydrogen and in vacuum. These results are also inexplicable by either of the existing theories. Thus it is proposed that carbon transfer from graphite to iron takes place through interparticle contacts after the adsorbed gases, which form a hindrance at the contacts, have been removed. The results of other authors have been explained in the light of this proposal. PM/0211
Superior hydrogen storage properties of LiBH4 catalyzed by Mg(AlH4)2 (2011)

Mg(AlH(4))(2) is found to provide a synergistic effect on improving the de-/rehydrogenation properties of LiBH(4). The Mg(AlH(4))(2)-catalyzed LiBH(4) exhibits lower dehydrogenation temperature and faster de-/rehydrogenation kinetics than the individually MgH(2)- or Al-catalyzed LiBH(4).
31.2: Invited Paper: Advanced Materials Development for Ink‐Jet Printed OLEDs (2019)

High‐performance, ink‐jet printable OLED materials have been successfully developed. Our wide libraries of platforms and small molecule hosts were effective for improving efficiency and lifetime of OLEDs. For wide color gamut, new emitters with deep in color and narrow full‐width at half maximum were developed. Further, solvent systems having physical properties suitable for improving film uniformity in pixels were found.
Learning Phrase Patterns for Text Classification (2013)

This paper introduces methods to discriminatively learn phrase patterns for use as features in text classification. An efficient solution is described using a recursive algorithm with a mutual information selection criterion. The algorithm automatically determines when word classes are useful in specific locations of a phrase pattern, allowing for variable specificity depending on the amount of labeled data available. Experiments are carried out on three text classification tasks in both English and Chinese, resulting in improved performance when adding the phrase patterns to the existing <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">n</i> -gram features.
Berm Method for Quantification of Infiltration at the Plot Scale in High Conductivity Soils (2013)

Measuring infiltration at the plot scale is difficult, especially for high hydraulic conductivity soils. At the plot scale, the infiltration rate is usually calculated by comparing surface runoff to rainfall. Direct measurement of infiltration beyond the point scale is typically limited to locations where land forming (e.g., infiltration pond) has been performed or fields with basin irrigation systems. The standard method for field measurement of point-scale infiltration is the double ring infiltrometer, which is limited in size (typically 30 cm diameter). In this research, a new method is proposed that uses a temporary berm constructed of a water-filled 15-cm diameter vinyl hose with the edges sealed to the soil using bentonite. The berm is capable of confining infiltration plot areas of various sizes (e.g., 1×1 and 3×3 m areas in this research). Water tanks with 0.8 and 4.9 m3 capacity were used to supply water to the plots by gravity flow. A constant head could be maintained within the plot using either an automatic float valve for lower infiltration rates or a manually operated gate valve for higher infiltration rates. Observation wells were installed outside the plots to monitor for water table rise and tracers that leached into the groundwater. Guidelines are provided for tank size and refilling frequency for conducting field experiments. The procedure was tested on soils ranging from silt loam to coarse gravel using 12 1×1 and 3×3 m plots at three alluvial floodplain sites. Measured infiltration rates ranged over two orders of magnitude (0.8–74 cm/h) and were typically greater than the estimated permeability of the limiting layer reported in soil surveys, suggesting the need for larger scale field measurements of infiltration rates.
Mechanisms of Zika virus antibody neutralization (2018)

Abstract Zika virus (ZIKV) infection during pregnancy has emerged as a global public health problem because of its ability to cause severe congenital disease. To gain insights into the humoral immune response against ZIKV, we set out to characterize a panel of neutralizing monoclonal antibodies (mAbs) with the goal of correlating B-cell epitopes with neutralizing potency. We developed six mouse mAbs against ZIKV including four that are ZIKV specific and neutralized infection of African, Asian, and American strains to varying degrees. The crystal structures of four of these mAbs in complex with DIII domain of the viral E protein revealed three spatially distinct epitopes, corresponding to the lateral ridge, C-C′ loop, and ABDE sheet regions. Of these, antibodies that target the lateral ridge epitope can confer protection in a lethal mouse model of ZIKV infection. Currently, we are focusing on a subset of human mAbs from healthy subjects with prior ZIKV infection. One of these human neutralizing mAbs binds an epitope in domain II, exhibits broad inhibitory activity in vitro, and protects pregnant and non-pregnant mice from ZIKV infection. Other neutralizing mAbs target DIII and show significant neutralizing activity in vitro. Strikingly, mechanistic studies indicate that the most potent murine and human mAbs in our panels are all capable of blocking pH triggered membrane fusion, and confocal microscopy studies indicate that they can traffic into target cells in complex with virions. These studies provide a path for developing prophylactic or therapeutic antibodies for use in pregnancy and designing epitope-specific vaccines against ZIKV.
Course teaching discuss of electromechanical specialty in independent college (2010)

Our country's electromechanical specialty is the industry of labor-intensive,capital-intensive,knowledge-intensive,technology-intensive and market-intensive,it requires the professionals with high quality,all of these requires new education model course for teaching of electromechanical specialty in independent college,and explores new construction and development.
Removing Polyvinylpyrrolidone from Catalytic Pt Nanoparticles without Modification of Superficial Order (2012)

Herein, we report a simple methodology for cleaning Pt nanoparticles, prepared by a colloidal synthesis and coated with polyvinylpyrrolidone, without loss of crystalline surface structure. To prove the removal of the polyvinylpirrolidone from a Pt surface without disturbing the superficial order, a cleaning method using a solution of H(2)O(2)/H(2)SO(4) was tested successfully for a Pt(111) single-crystal electrode. The decontamination method was then tested for two different types of nanoparticles by mixing the suspension of nanoparticles with H(2)O(2)/H(2)SO(4) and subsequent centrifugation. The resulting voltammetric profiles of platinum particles synthesized by the colloidal method employing PVP show a marked presence of the adsorption-state characteristic of (111) ordered surface domains. The presence of the well-ordered domains present on the surface of the nanoparticles was confirmed by using the irreversible adsorption of bismuth, tellurium and germanium.
A Case Against Hardware Managed DRAM Caches for NVRAM Based Systems (2021)

Non-volatile memory (NVRAM) based on phase-change memory (such as Optane DC Persistent Memory Module) is making its way into Intel servers to address the needs of emerging applications that have a huge memory footprint. These systems have both DRAM and NVRAM on the same memory channel with the smaller capacity DRAM serving as a cache to the larger capacity NVRAM in the so called 2LM mode. In this work we analyze the performance of such DRAM caches on real hardware using a broad range of synthetic and real-world benchmarks. We identify three key limitations of DRAM caches in these emerging systems which prevent large-scale, bandwidth bound applications from taking full advantage of NVRAM read and write bandwidth. We show that software based techniques are necessary for orchestrating the data movement between DRAM and PMM for such workloads to take full advantage of these new heterogeneous memory systems.
Sequence Requirements for Rev Multimerization in Vivo (1994)

Multimerization of the human immunodeficiency virus type 1 (HIV-1) Rev protein is believed to be critical to its biological activity. However, the precise protein sequence requirements for Rev multimerization in vivo, and whether multimerization is facilitated by specific RNA binding or vice versa, has remained controversial. In this report, we describe a sensitive in vivo assay for the multimerization of HIV-1 Rev on its cognate RRE primary RNA binding site. Using this assay, we demonstrate that an intact Rev arginine-rich domain, while critical to specific RNA binding, is dispensable for multimerization on the RRE. Mutations introduced into Rev sequences that flank this basic domain produce a partial multimerization phenotype in vivo even though these mutations are known to block Rev multimerization in vitro. Similarly, mutations introduced into the leucine-rich activation domain of Rev, which appear to have no effect on in vitro multimerization, also markedly inhibit multimerization of Rev on the RRE in vivo. Overall, these data appear consistent with the hypothesis that in vivo formation of the multimeric Rev:RRE ribonucleoprotein complex is facilitated by both the RRE RNA substrate and, as first proposed by Bogerd and Greene U. Virol. 67, 2496-2502, 1993), by bridging by a cellular cofactor for Rev that likely interacts with multiple Rev activation domains.
Surface-emitting GaAlAs/GaAs laser with etched mirrors (1986)

A surface-emitting GaAlAs/GaAs laser is fabricated using ion milling to etch the mirrors. Output light is defected to a direction perpendicular to the wafer surface by a monolithically integrated 45° mirror.
A new load balancing strategy by task allocation in edge computing based on intermediary nodes (2020)

Abstract The latency of cloud computing is high for the reason that it is far from terminal users. Edge computing can transfer computing from the center to the network edge. However, the problem of load balancing among different edge nodes still needs to be solved. In this paper, we propose a load balancing strategy by task allocation in edge computing based on intermediary nodes. The intermediary node is used to monitor the global information to obtain the real-time attributes of the edge nodes and complete the classification evaluation. First, edge nodes can be classified to three categories (light-load, normal-load, and heavy-load), according to their inherent attributes and real-time attributes. Then, we propose a task assignment model and allocate new tasks to the relatively lightest load node. Experiments show that our method can balance load among edge nodes and reduce the completion time of tasks.
Design and Analysis of a Resonant Reactive Shield for a Wireless Power Electric Vehicle (2014)

In this paper, we propose the concept and design methodology for a resonant reactive shield for the reduction of magnetic field leakage from a wireless power transfer (WPT) systems. By using LC resonance, the reactive shield can generate a cancelling magnetic field to reduce the incident magnetic field from WPT coils and effectively reduce the total magnetic field without consuming additional power. The shielding effectiveness of the resonant reactive shield and its effect on WPT efficiency are analyzed with simulation and measurements. For practical application to wirelessly charged electric vehicles, an automatic tuning system for the resonant reactive shield is also proposed and implemented. The effectiveness of a resonant reactive shielding is verified by experiments in a wirelessly charged electric bus.
Epigenetics in heart failure phenotypes (2016)

Chronic heart failure (HF) is a leading clinical and public problem posing a higher risk of morbidity and mortality in different populations. HF appears to be in both phenotypic forms: HF with reduced left ventricular ejection fraction (HFrEF) and HF with preserved left ventricular ejection fraction (HFpEF). Although both HF phenotypes can be distinguished through clinical features, co-morbidity status, prediction score, and treatment, the clinical outcomes in patients with HFrEF and HFpEF are similar. In this context, investigation of various molecular and cellular mechanisms leading to the development and progression of both HF phenotypes is very important. There is emerging evidence that epigenetic regulation may have a clue in the pathogenesis of HF. This review represents current available evidence regarding the implication of epigenetic modifications in the development of different HF phenotypes and perspectives of epigenetic-based therapies of HF.
Pour une socioéconomie engagée (2018)

Ce livre engage un dialogue sur les principaux concepts et objets de recherche développés par Jean-Michel Servet, à travers ses contributions à une socioéconomie de la monnaie, de la finance et des alternatives.
Dynamic Theory of Liquid Junction Potentials (2009)

A Nernst-Planck-Poisson finite difference simulation system is used to model the dynamic evolution of a liquid junction from a nonequilibrium initial condition to a condition of steady potential difference, in a linear semi-infinite space. Liquid junctions of Lingane's type 1 (monophasic, unequal concentration) and type 2 (bi-ionic potential; biphasic, equal concentration) are considered, for the sake of simplicity. Analysis of the results shows consistency with known and novel asymptotic solutions. A comprehensive dynamic theory of the free liquid junction potential is presented, having considered the simulated concentration profiles and electric field in the system. This reveals a dynamically relaxing junction in which a diffuse layer continues to expand. This is advocated as physically realistic and shown to be consistent with a steady state potential difference, which arises after 10-1000 ns for typical aqueous systems, when the expanding diffuse layer has a corresponding size of 10-1000 nm. Hence, Planck's concept [Wied. Ann. 1890, 40, 561-576] that a steady state potential difference exclusively implies a static junction with equal fluxes of all species is shown to be false, for an unconstrained system.
Detection of Neuroblastoma Cells in Human Bone Marrow Using a Combination of Monoclonal Antibodies (1982)

Two different types of monoclonal antibodies, antineuroblastoma (PI153/3), and antilymphocyte (P3B1-C3) were used to identify and classify tumor cells in the bone marrow of patients with neuroblastoma and with other types of cancer. Cells expressing the antigens were detected with peroxidase-coupled anti-Ig. The cell-surface labeling is manifested as a dense black precipitate at the membrane visualized by light microscopy. The combination of the two antibodies gives specific staining patterns for each cell type. PI153/3+, P3B1-C3- is specifically associated with neuroblastoma cells. PI153/3+,P3B1-C3+ is expressed on blast cells from some types of acute lymphoblastic leukemia and a small subpopulation of normal lymphocytes. These monoclonal antibodies thus allow specific visual detection of single neuroblastoma cells in bone marrow samples. The results demonstrate how combinations of monoclonal antibodies can be effectively used to identify specific cell types by their expression of and lack of specific marker determinants. Application of this principle is particularly relevant for dissecting populations of related cells and/or molecules.