gebhart/patrepeval huggingface dataset:
IN VIVO MOTION OF THE HUMAN CERVICAL SPINAL CORD IN EXTENSION AND FLEXION (1992)

The objective of this study was to use a motion tracking magnetic resonance (MR) imaging technique in order to measure the in vivo quasi-static motion of the cervical spinal cord in human volunteers produced by varying degrees of flexion and extension. An initial preparation pulse consisting of a slice selective 180 degree pulse inverted the magnetization in a midsagittal section while the subject was in a neutral position. Before motion, tissue was tagged in multiple transverse planes using additional spatially selective radio frequency pulses. After the head-neck structure came to rest in its new position, a midsagittal MR image was obtained. This showed the movement of the tissue tag lines caused by the maneuver. A comparison of the tagged line pattern in MR images obtained with and without head movement revealed both the distortion of the cervical spinal cord and its displacement relative to surrounding structures. The results revealed the spinal cord moves and deforms considerably during the flexion and extension maneuvers within the normal range of motion. These in vivo motion measurements form the foundation for more sophisticated studies to elucidate the biomechanical mechanisms responsible for traumatic spinal cord injury. For the covering abstract of the conference see IRRD 871381.
An economic model predictive control approach to integrated production management and process operation (2016)

Managing production schedules and tracking time‐varying demand of certain products while optimizing process economics are subjects of central importance in industrial applications. We investigate the use of economic model predictive control (EMPC) in tracking a production schedule. Specifically, given that only a small subset of the total process state vector is typically required to track certain scheduled values, we design a novel EMPC scheme, through proper construction of the objective function and constraints, that forces specific process states to meet the production schedule and varies the rest of the process states in a way that optimizes process economic performance. Conditions under which feasibility and closed‐loop stability of a nonlinear process under such an EMPC for schedule management can be guaranteed are developed. The proposed EMPC scheme is demonstrated through a chemical process example in which the product concentration is requested to follow a certain production schedule. © 2016 American Institute of Chemical Engineers AIChE J , 63: 1892–1906, 2017
Reducing processor power consumption by improving processor time management in a single-user operating system (1996)

The CPU is one of the major power consumers in a portable computer, and considerable power can be saved by turning off the CPU when it is not doing useful work. In Apple's MacOS, however, idle time is often converted to busy waiting, and generally it is very hard to tell when no useful computation is occurring. In this paper, we suggest several heuristic techniques for identifying this condition, and for temporarily putting the CPU in a low-power state. These techniques include turning off the processor when all processes are blocked, turning off the processor when processes appear to be busy waiting, and extending real time process sleep periods. We use trace-driven simulation, using processor run interval traces, to evaluate the potential energy savings and performance impact. We find that these techniques save considerable amounts of processor energy (as much as 66% ), while having very little performance impact (less than 2% increase in run time). Implementing the proposed strategies should increase battery lifetime by approximately 20% relative to Apple's current CPU power management strategy, since the CPU and associated logic are responsible for about 32% of power use; similar techniques should be applicable to operating systems with similar behavior.
Some properties of a .BETA.-cyanoalanine-forming enzyme of Enterobacter sp. 10-1. (1982)

The properties of a purified β-cyanoalanine (β-CNAla)-forming enzyme isolated from Enterobacter sp. 10-1 were studied. It has been found previously that this enzyme catalyzed the formation of cysteine from O-acetyl-L-serine and soldium sulfide, as well as the formation of β-CNAla from O-acetyl-L-serine and sodium cyanide. The affinity of the enzyme for sodium sulfide in the formation of cysteine was one order of magnitude greater than that for sodium cyanide in the β-CNAla formation. In addition, the optimum pH, pH- and thermal stability of β-CNAla-forming activity were similar to those of cysteine-forming activity. The results of the studies indicated that the β-CNAla-forming enzyme of Enterobacter sp. 10-1 was an O-acetylserine sulfhydrylase catalyzing the formation of cysteine from O-acetyl-L-serine and sulfide. The solution of the enzyme was yellow and exhibited an absorption peak at 420 nm. The enzyme itself contained approximately 1 mol of pyridoxal phosphate per 34, 000 g of protein.
Detecting protein variants by mass spectrometry: a comprehensive study in cancer cell-lines (2017)

We discuss in detail the technical and computational challenges in identifying variant peptides by MS and show that uncovering these variants allows the identification of druggable mutations within important cancer genes.
Über gesellschaftliche Ohnmacht und Utopie in Christa WolfsKassandra (1985)

(1985). Uber gesellschaftliche Ohnmacht und Utopie in Christa Wolfs Kassandra. Oxford German Studies: Vol. 16, No. 1, pp. 109-121.
Synthesis of enantiomerically pure C2-symmetric 4-pyrrolidinopyridine derivative as a chiral acyl transfer catalyst for the kinetic resolution of secondary alcohols (2000)

The first chiral C2-symmetric 4-pyrrolidinopyridine (PPY) derivative was synthesized in an enantiomerically pure form and successfully utilized as a chiral nucleophilic catalyst for the kinetic resolution of secondary alcohols leaving one enantiomer with high selectivity factors (up to s = 13.5).
Baseline Concentrations of 15 Trace Elements in Florida Surface Soils (1999)

Abstract The objective of this study was to establish baseline concentrations for 15 potentially toxic elements (Ag, As, Ba, Be, Cd, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Sb, Se, and Zn) based on 448 representative Florida surface soils using microwave assisted HNO 3 ‐HCl‐HF digestion. Baseline concentrations of those elements were (mg kg −1 ): Ag 0.07–2.50, As 0.02–7.01, Ba 1.67–112, Be 0.04–4.15, Cd 0–0.33, Cr 0.89–80.7, Cu 0.22–21.9, Hg 0.00075–0.0396, Mo 0.13–6.76, Ni 1.70–48.5, Pb 0.69–42.0, Sb 0.06–0.79, Se 0.01–1.11, and Zn 0.89–29.6, respectively. Upper baseline values for most elements corresponded with these reported in literature, except Ba, Hg, Mn, Sb, and Zn, which were 3 to 23 times lower. Soil properties, including pH, organic carbon (OC), particle size, cation‐exchange capacity (CEC), available water, extractable base, extractable acidity, total Ca, Mg, P, K, Fe, and Al concentrations, were related to metal concentrations using factorial analysis. Eight factors were identified (total Fe and Al, CEC, pH, clay, OC, total Ni and Mo, total Sb and Pb, and total Hg) and accounted for 87% of the total variance, suggesting that metal concentrations were primarily controlled by soil compositions. Multiple regression of elemental concentrations against total Fe, total Al, clay, OC, CEC, and pH was significant for all elements. Partial correlation coefficients indicated that total Fe and/or Al explained most of the variance for Mn, Ni, Ba, Be, Hg, As, Cd, Cr, Cu, Mo, Pb, and Zn concentrations. Most of the variance in Se was related to clay, whereas those of Ag and Sb related to clay and total Al.
The Neutral Constituents of the Cactus Lophocereus schottii. The Structure of Lophenol--4α-Methyl-Δ7-cholesten-3β-ol--A Link in Sterol Biogenesis1-3 (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Neutral Constituents of the Cactus Lophocereus schottii. The Structure of Lophenol--4α-Methyl-Δ7-cholesten-3β-ol--A Link in Sterol Biogenesis1-3Carl Djerassi, G. W. Krakower, A. J. Lemin, Liang H. Liu, J. S. Mills, and R. VillottiCite this: J. Am. Chem. Soc. 1958, 80, 23, 6284–6292Publication Date (Print):December 1, 1958Publication History Published online1 May 2002Published inissue 1 December 1958https://pubs.acs.org/doi/10.1021/ja01556a031https://doi.org/10.1021/ja01556a031research-articleACS PublicationsRequest reuse permissionsArticle Views176Altmetric-Citations67LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Phospho-Ser727 triggers a multistep inactivation of STAT3 by rapid dissociation of pY705–SH2 through C-terminal tail modulation (2019)

Signal transducer and activator of transcription 3 (STAT3) is involved in many biological processes, including immunity and cancer. STAT3 becomes phosphorylated at Tyr705 and Ser727 on IL-6 stimulation. Phospho-Tyr705 (pY705) stabilizes the STAT3 dimer with reciprocal interactions between pY705 and the SH2 of the other molecule and phospho-Ser727 (pS727) accelerates pY705 dephosphorylation. We study how pS727 regulates STAT3 in both structural and biological perspectives. Using STAT3 reconstituted in HepG2-stat3-knockout cells, we show that pS727, together with a handshake N-terminal domain (NTD) interaction, causes rapid inactivation of STAT3 for pY705 dephosphorylation and a chromosome region maintenance 1 (CRM1)-independent nuclear export, which is critical for faithful STAT3 response to the cellular signals. The various N-terminal tags, GFP-related Ruby and FLAG, rendered the export CRM1-dependent and especially FLAG-tag caused nuclear accumulation of STAT3, indicating the presence of conformational changes in inactivation. Impaired reactivation of STAT3 by S727A or FLAG-tag delayed or inhibited the IL-6-induced saa1 mRNA expression, respectively. The detailed analysis of the pY705-SH2 structure identified the C-terminal tail (CTT) from L706 to P715 as a key regulator of the CTT-CTT intermolecular and the CTT-SH2 intramolecular interactions that support pY705-SH2 association. The functional studies using multiple STAT3 mutants indicated that the degree of the two interactions determines the stability of pY705-SH2 interaction. Importantly, Pro715 was critical for the pS727's destabilizing activity and the known phosphorylation and acetylation at the CTT structurally inhibited the pY705-SH2 interaction. Thus, pS727 triggers pY705-SH2 dissociation by weakening the supportive interactions likely through CTT modulation, inducing rapid cycles of STAT3 activation-inactivation for proper function of STAT3.
Increased T cell breadth and antibody response elicited in prime-boost regimen by viral vector encoded homologous SIV Gag/Env in outbred CD1 mice (2016)

These results contrast with related studies using Env or Gag as the sole antigen and suggest that prime-boost immunizations based on homologous SIVmac239 Gag inserts is an efficient component of genetic VLP vaccines-both for induction of potent antibody responses and cross-reactive CD8+ T cell responses.
Copper-Mediated Living Radical Polymerization (Atom Transfer Radical Polymerization and Copper(0) Mediated Polymerization): From Fundamentals to Bioapplications (2015)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCopper-Mediated Living Radical Polymerization (Atom Transfer Radical Polymerization and Copper(0) Mediated Polymerization): From Fundamentals to BioapplicationsCyrille Boyer*†‡, Nathaniel Alan Corrigan‡, Kenward Jung‡, Diep Nguyen‡, Thuy-Khanh Nguyen‡, Nik Nik M. Adnan†‡, Susan Oliver†‡, Sivaprakash Shanmugam‡, and Jonathan Yeow†‡View Author Information† ‡ †Australian Centre for Nanomedicine, and ‡Centre for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney 2052, Australia*E-mail: [email protected]Cite this: Chem. Rev. 2016, 116, 4, 1803–1949Publication Date (Web):November 4, 2015Publication History Received6 July 2015Published online4 November 2015Published inissue 24 February 2016https://doi.org/10.1021/acs.chemrev.5b00396Copyright © 2015 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceArticle Views37471Altmetric-Citations372LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (85 MB) Get e-AlertsSUBJECTS:Copolymers,Micelles,Polymerization,Polymers,Radical polymerization Get e-Alerts
ChemInform Abstract: Asymmetric Rh(II)‐Catalyzed Cyclopropanation of Alkenes with Diacceptor Diazo Compounds: p‐Methoxyphenyl Ketone as a General Stereoselectivity Controlling Group. (2011)

Abstract The reaction features the use of p‐methoxyphenyl ketone as a diastereo‐ and enantioselective controlling group for the synthesis of a series of cyclopropanes.
Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems (2006)

Although the cold-shock response has now been studied in a number of different organisms for several decades, it is only in the last few years that we have begun to understand the molecular mechanisms that govern adaptation to cold stress. Notably, all organisms from prokaryotes to plants and higher eukaryotes respond to cold shock in a comparatively similar manner. The general response of cells to cold stress is the elite and rapid overexpression of a small group of proteins, the so-called CSPs (cold-shock proteins). The most well characterized CSP is CspA, the major CSP expressed in Escherichia coli upon temperature downshift. More recently, a number of reports have shown that exposing yeast or mammalian cells to sub-physiological temperatures (<30 or <37 degrees C respectively) invokes a co-ordinated cellular response involving modulation of transcription, translation, metabolism, the cell cycle and the cell cytoskeleton. In the present review, we summarize the regulation and role of cold-shock genes and proteins in the adaptive response upon decreased temperature with particular reference to yeast and in vitro cultured mammalian cells. Finally, we present an integrated model for the co-ordinated responses required to maintain the viability and integrity of mammalian cells upon mild hypothermic cold shock.
Binding of THZif-1, a MAZ-like Zinc Finger Protein to the Nuclease-hypersensitive Element in the Promoter Region of the c-MYC Protooncogene (1996)

A detailed analysis is reported of the binding of the zinc finger protein THZif-1 to the nuclease-hypersensitive element (NHE) in the promoter region of the c-MYC gene using the electrophoretic mobility shift assay and a series of mutants of a fusion protein composed of glutathione S-transferase and THZif-1. The THZif-1 protein bound specifically to the single-stranded (ss) pyrimidine-rich DNA of the NHE (ss c-myc NHE-C) with an apparent dissociation constant (Kd (app)) of 0.077 microM. By contrast, no binding to the single-stranded purine-rich DNA of the NHE (ss c-myc NHE-G) was detected. Moreover, the binding affinity of THZif-1 protein was 2-fold higher for the single-stranded 5-methyl-2'-deoxycytidine derivative of NHE (ss c-myc NHE-me5C) than for the unmethylated NHE. In the case of the binding of THZif-1 to methylated double-stranded (ds) NHE (ds c-myc NHE-me5CG), no significant binding to the DNA was observed. The decrease in binding to DNA of THZif-1 was significant in the case of mutated ds c-myc NHE, in which more than two sites of deoxycytidine residues were methylated. However, the binding affinity of THZif-1 protein for methylated and for unmethylated triple-helical DNA of the NHE was almost identical. Moreover, the domain of the THZif-1 protein that made the major contribution to binding to ss c-myc NHE-C or ss c-myc NHE-me5C corresponded to the amino-terminal second zinc finger motif. Taken together, the results indicate that the THZif-1 protein exhibits preferential DNA-binding activity with ss c-myc NHE-C, ds c-myc NHE-CG, and ts c-myc NHE but not with ss c-myc NHE-G and ds c-myc NHE-me5CG in vitro.
Point: Mammography Screening—Sticking to the Science (2015)

BACKGROUND [...]
A New and Rapid Method for Determination of Beer-Spoilage Ability of Lactobacilli (1997)

A new method for determining the capability of beer-spoilage by lactobacilli was developed. This method is based on the sequence of a hop possible-resistance gene, designated horA (1,749base), which was identified on a plasmid of hop-resistant Lactobacillus brevis ABBC45. A pair of primers were designed based on the partial sequence of horA. We examined various Lactobacillus species for the presence of horA-like gene by polymerase chain reaction (PCR) using these primers. The amplification of specific DNA fragments, which suggests the presence of horA-like gene, occurred almost exclusively in the beer-spoilage lactobacilli regardless of their species. This method makes it possible to determine the capability of beer-spoiling character of lactobacilli rapidly, without taxonomic identification and the cultivation in beer or hop containing media.
Risk Factors for Ectopic Pregnancy in Women With Symptomatic First-Trimester Pregnancies (2006)

Approximately one in 50 pregnancies in the United States is ectopic, and the incidence of ectopic pregnancy (EP) reportedly has increased 6-fold over the past 25 years. Early diagnosis and appropriate outpatient care clearly limit morbidity and mortality from EP as well as helping to preserve future fertility. This study is the first to associate both historical variables and symptoms with the presence of EP in women who present in the first trimester with pain and/or bleeding but in whom the diagnosis is not readily apparent. A total of 2026 such women, seen at a single center in the years 1990 through 1999, were evaluated. EP was diagnosed in 367 patients, ongoing intrauterine pregnancy in 467, and spontaneous abortion (SAB) in 1192. Case patients were slightly older than controls in this largely black study cohort. A large number of possible confounding factors were adjusted for, including age, voluntary interruption of pregnancy in the past, the number of ectopic gestations, a history of pelvic inflammatory disease (PID), parity, moderate to severe bleeding at presentation, pain as the presenting symptom, and the initial level of human chorionic gonadotropin (hCG). A history of EP was positively associated with current EP as were an initial hCG level exceeding 500 mIU/mL, parity of one, past hospitalization for PID, and presentation with either pain or moderate to severe bleeding. Age less than 25 and a history of voluntarily interrupting pregnancy both were negatively associated with EP. Putative risk factors for EP that were not significant factors in the present study included gravidity, previous SAB, previous live births, the past use of an intrauterine device, previous pelvic surgery or cesarean section, and either a history of or current cervical infection with chlamydia and/or gonorrhea. If the risk factors for EP identified in this study do have high predictive value, they may aid the timely diagnosis of this condition, permit medical treatment, lower morbidity and mortality, preserve fertility, and lessen healthcare costs.
Isolation of Intact Protein Storage Vacuoles from Barley Aleurone (Identification of Aspartic and Cysteine Proteases) (1996)

Within the cereal aleurone reserve proteins are stored in specialized organelles, the protein storage vacuoles (PSV). We developed an aqueous method for the isolation of intact PSV. Barley (Hordeum vulgare L. cv Himalaya) aleurone protoplasts were gently lysed by passing them through a syringe needle. PSV were separated from cytoplasmic components by microfiltration and low-speed centrifugation. Isolated PSV appeared by light microscopy to be identical with those within barley aleurone protoplasts. Luminal contents were retained throughout the isolation procedure. We used isolated PSV to identify and characterize PSV-associated proteolytic activities. Isolated PSV contained cysteine proteases and aspartic proteases (APs). Gibberellic acid treatment of protoplasts increased cysteine protease activity. Protein blots probed with anti-H. vulgare aspartic proteinase (HvAP) indicated that one PSV-AP was HvAP. Immunocytochemical localization by electron microscopy confirmed the presence of HvAP within the lumen of PSV. We conclude that isolated barley aleurone PSV will be useful in further characterizing this organelle.
Prolonged Serologically Active Clinically Quiescent Systemic Lupus Erythematosus: Frequency and Outcome (2010)

Fifty-nine percent of SACQ patients experienced flare, but after a median of 3 years. Fluctuations in complement and anti-dsDNA levels did not predict flare, thus treatment decisions in these patients must rely upon close clinical observation. Alternative predictive biomarkers warrant study.