gebhart/patrepeval huggingface dataset:
Personalized Cardiovascular Regenerative Medicine: Targeting the Extreme Stages of Life (2019)

Cardiovascular regenerative medicine is an exciting new approach that promises to change the current care of million people world-wide. Major emphasis was given to the quality and quantities of regenerative products, but recent evidence points to the importance of a better specification of the target population that may take advantage of these advanced medical treatments. Patient stratification is an important step in drug development. Tailoring treatment to the patient's specificity allowed significant improvement in cancer therapy, but personalized regenerative medicine is still at the initial stage in the cardiovascular field. For example, new-borns with a congenital heart condition and elderly people require dedicated therapeutic approaches, which adapt to their lifetime needs. In these people, personalized treatments may overcome the benefits delivered by standard protocols. In this review, we provide insights into these extreme stages of life as potential targets for cardiovascular reconstitution.
Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations (2004)

ADVERTISEMENT RETURN TO ISSUEPREVFeaturesNEXTPeer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular SeparationsShear-force methods are an alternative to pressure- and voltage-gradient methods.David Clicq, Kris Pappaert, Sarah Vankrunkelsven, Nico Vervoort, Gino V. Baron, and and Gert DesmetCite this: Anal. Chem. 2004, 76, 23, 430 A–438 APublication Date (Web):December 1, 2004Publication History Published online1 December 2004Published inissue 1 December 2004https://pubs.acs.org/doi/10.1021/ac0416822https://doi.org/10.1021/ac0416822newsACS Publications. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views354Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (294 KB) Get e-Alertsclose SUBJECTS:Chromatography Get e-Alerts
The Tyrosine Kinase Receptor RET Interacts in Vivo with Aryl Hydrocarbon Receptor-Interacting Protein to Alter Survivin Availability (2009)

The identification of the AIP-RET complex represents a starting point to study key cellular processes involved in RET-induced apoptosis.
Octakis(3‐azidopropyl)octasilsesquioxane: A Versatile Nanobuilding Block for the Efficient Preparation of Highly Functionalized Cube‐Octameric Polyhedral Oligosilsesquioxane Frameworks Through Click Assembly (2010)

A one-step synthesis of octakis(3-azidopropyl)octasilsesquioxane from commercially available octakis(3-aminopropyl)octasilsesquioxane has been developed through a highly efficient diazo-transfer reaction under very mild conditions. Nonaflyl azide is shown to be a safer, cheaper, and more efficient reagent for this transformation than the better known and generally used diazo-transfer reagent triflyl azide. Octakis(3-azidopropyl)octasilsesquioxane is an excellent nanobuilding block that can be readily octafunctionalized with a range of terminal alkynes by copper(I)-catalyzed 1,3-dipolar azide-alkyne cycloaddition to provide new functional nanocages, maintaining a perfect 3D cubic symmetry. The mildness, simplicity, and efficiency of this approach have been demonstrated in the preparation of a glyco-polyhedral oligosilsesquioxane (POSS) conjugate and a BODIPY-POSS cluster (BODIPY = boron dipyrromethene).
Review of shock-induced supersonic combustion research and hypersonic applications (1994)

This article focuses on research in supersonic and kinetics in high-speed flow between 1959-1968, and the application of the experimental results to hypersonic propulsion. The analysis discusses both advantages and problems for premixing the fuel and employing as an ignition method for a scramjet flying at a high Mach number. The experimental tests are discussed, including implications to the chemical kinetics of the high-velocity process. The conditions were confined to relatively low pressure, less than 2 atm (200 kPa). The results were considered to be mainly applicable for high-altitude scramjet flight, at low static pressure, where chemical reaction distances will be long. At these lower pressures, shock-induced combustion may be the predominant effect in a scramjet application, and it has some advantages that are discussed. The relation between and is also discussed. In addition, an attempt is made to resolve the conflicting experimental data published in the 1960s relating to standing detonation waves and combustion.
Formation of Inclusion Complexes of Poly(3-hydroxybutyrate)s with Cyclodextrins. 1. Immobilization of Atactic Poly(R,S-3-hydroxybutyrate) and Miscibility Enhancement between Poly(R,S-3-hydroxybutyrate) and Poly(ε-caprolactone) (2002)

Atactic poly(R,S-3-hydroxybutyrate) (a-PHB) was synthesized by anionic polymerization of β-butyrolactone with potassium methoxide as an initiator. This completely amorphous polyester is capable of forming a crystalline inclusion complex (IC) with γ-cyclodextrin (γ-CD) adopting a channel structure. There is no evidence showing that a-PHB may form IC with either α-CD or β-CD. On the basis of these discoveries, a common IC was formed with two polymer chains, a-PHB and poly(ε-caprolactone) (PCL), randomly distributed into the channels of γ-CD-PCL/a-PHB IC crystals. Nevertheless, in the formation of the common IC, PCL inclusion appears superior to a-PHB inclusion. Therefore, the molar ratio of a-PHB and PCL in the coalesced sample has been detected to be lower than that used in the formation of the common IC. Washing the common IC with hot water removed the γ-CD, and the molecular chains of the two polymers were coalesced. Very interestingly, only a single glass transition temperature (Tg), dependent on the composition, was observed between the Tg's of a-PHB (∼5 °C) and PCL (∼−60 °C) in the differential scanning calorimetry (DSC) measurements of the coalesced samples. To the contrary, Tg of a-PHB (∼5 °C) was found to remain unchanged in the physical blend with PCL. Tm's of coalesced blend samples are lower than that of pure PCL, while the Tm of the physical blend is almost the same as that of pure PCL. These results strongly demonstrate that the miscibility of the inherently immiscible a-PHB/PCL pair has been improved in the coalesced blends. Fourier transform infrared (FTIR), thermogravimetric analysis (TGA), 13C solid-state NMR, DSC, and wide-angle X-ray diffraction (WAXD) measurements were employed to demonstrate IC formation.
Customized Real-Time First-Order Methods for Onboard Dual Quaternion-based 6-DoF Powered-Descent Guidance (2023)

View Video Presentation: https://doi.org/10.2514/6.2023-2003.vid The dual quaternion-based 6-DoF powered-descent guidance algorithm (DQG) was selected as the candidate powered-descent guidance algorithm for NASA’s Safe and Precise Landing — Integrated Capabilities Evolution (SPLICE) project. DQG is capable of handling state-triggered constraints that are of the utmost importance in terms of enabling technologies such as terrain relative navigation (TRN). In this work, we develop a custom solver for DQG to enable onboard implementation for future rocket landing missions. We describe the design and implementation of a real-time-capable optimization framework, called sequential conic optimization (SeCO), that blends together sequential convex programming and first-order conic optimization to solve difficult nonconvex trajectory optimization problems, such as DQG, in real-time. This framework is entirely devoid of matrix factorizations/inversions, making it suitable for safety-critical applications. Under the hood, the SeCO framework leverages a first-order primal-dual conic optimization solver, based on the proportional-integral projected gradient method (PIPG), that combines the ideas of projected gradient descent and proportional-integral feedback of constraint violation. Unlike other conic optimization solvers, PIPG effectively exploits the sparsity and geometric structure of the constraints, avoids expensive equation solving, and is suitable for both real-time and large-scale applications. We describe the implementation of this solver, and develop customizable first-order methods, including an analytical preconditioning algorithm, to solve the nonconvex DQG optimal control problem in real-time. Strategies such as warm-starting and extrapolation are leveraged to further accelerate convergence. We show that the DQG-customized solver is able to solve the problem significantly faster than other state-of-the-art convex optimization solvers, and thus demonstrate the viability of SeCO for real-time, mission-critical applications onboard computationally constrained flight hardware.
Sequential Changes of the Connective Matrix Components of the Myocardium (Fibronectin and Laminin) and Evolution of Cardiac Fibrosis in Mice Infected with Trypanosoma cruzi (1989)

Interstitial matrix alterations due to chronic Trypanosoma cruzi myocarditis were studied in mice by immunofluorescent microscopy with specific purified antibodies against the main different collagen isotypes, laminin and fibronectin. During the early subacute stage (26-30 days postinfection), sarcolemmal and perivascular deposits of laminin and fibronectin were prominent. The presence of fibronectin appeared to correlate with the presence of inflammatory cells. By the late subacute phase and early chronic phase (50-90 and 80-90 days postinfection, respectively), laminin and Type IV collagen were present. These were the principal features, although fibronectin continued to be found among inflammatory cells, and pro-III and III collagens formed irregular bands and periarteriolar deposits. During the late chronic phase (150-200 days postinoculation) the interstitium was enlarged and irregular, with positive staining for laminin, Types III, pro-III, and IV collagens; fibronectin appeared as focal, subendocardial, interstitial, and perivascular deposits. The relative absence of Type I collagen and the apparent positive correlation between interstitial matrix amplification and the presence of mononuclear inflammatory cells suggest that fibrotic changes in chronic T. cruzi myocarditis can be reversed if the inflammatory changes subside.
Simultaneously formed storage node contact and metal contact cell (SSMC) for 1 Gb DRAM and beyond (2002)

Simultaneously formed Storage node contact and Metal contact Cell (SSMC) was investigated and developed with 0.18 /spl mu/m advanced KrF lithography as a promising candidate for the cell structure of 1 Gb DRAM and beyond, such as 4 Gb and 16 Gb DRAMs. SSMC can provide fast and reliable memory cell operation by reducing parasitic resistance between memory cell storage node and access transistor. Also SSMC can reduce the processing steps compared to the conventional COB (Capacitor Over Bit line) cell by forming storage node contact holes and metal contact hole at the same time. Furthermore, it is found that SSMC has many other advantages in terms of process margin, and wide application, for example in EML (embedded memory logic). Thus, SSMC is a promising cell structure for 1 Gb DRAM and beyond.
Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci (2014)

3D-SIM provides experimental evidence for profound differences between the functional architecture of transcriptionally active CTs and the Barr body. Basic structural features of CT organization such as CDCs and IC channels are however still recognized, arguing against a uniform compaction of the Barr body at the nucleosome level. The localization of distinct Xist RNA foci at boundaries of the rudimentary ANC may be considered as snap-shots of a dynamic interaction with silenced genes. Enrichment of SAF-A within Xi territories and its close spatial association with Xist RNA suggests their cooperative function for structural organization of Xi.
Sunitinib potentiates the cytotoxic effect of electrochemotherapy in pancreatic carcinoma cells (2022)

ECT and sunitinib combined treatment has clinical potential, and further studies are warranted.
Efficient Bayes-Adaptive Reinforcement Learning using Sample-Based Search (2012)

Bayesian model-based reinforcement learning is a formally elegant approach to learning optimal behaviour under model uncertainty, trading off exploration and exploitation in an ideal way. Unfortunately, finding the resulting Bayes-optimal policies is notoriously taxing, since the search space becomes enormous. In this paper we introduce a tractable, sample-based method for approximate Bayes-optimal planning which exploits Monte-Carlo tree search. Our approach outperformed prior Bayesian model-based RL algorithms by a significant margin on several well-known benchmark problems -- because it avoids expensive applications of Bayes rule within the search tree by lazily sampling models from the current beliefs. We illustrate the advantages of our approach by showing it working in an infinite state space domain which is qualitatively out of reach of almost all previous work in Bayesian exploration.
Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10 (2018)

Salmonella enterica is an important foodborne pathogen that uses secreted effector proteins to manipulate host pathways to facilitate survival and dissemination. Different S. enterica serovars cause disease syndromes ranging from gastroenteritis to typhoid fever and vary in their effector repertoire. We leveraged this natural diversity to identify stm2585, here designated sarA (Salmonella anti-inflammatory response activator), as a Salmonella effector that induces production of the anti-inflammatory cytokine IL-10. RNA-seq of cells infected with either ΔsarA or wild-type S. Typhimurium revealed that SarA activates STAT3 transcriptional targets. Consistent with this, SarA is necessary and sufficient for STAT3 phosphorylation, STAT3 inhibition blocks IL-10 production, and SarA and STAT3 interact by co-immunoprecipitation. These effects of SarA contribute to intracellular replication in vitro and bacterial load at systemic sites in mice. Our results demonstrate the power of using comparative genomics for identifying effectors and that Salmonella has evolved mechanisms for activating an important anti-inflammatory pathway.
Probabilistic programming (2014)

Probabilistic programs are usual functional or imperative programs with two added constructs: (1) the ability to draw values at random from distributions, and (2) the ability to condition values of variables in a program via observations. Models from diverse application areas such as computer vision, coding theory, cryptographic protocols, biology and reliability analysis can be written as probabilistic programs.
Micronutrient optimization for tissue engineered articular cartilage production of type II collagen (2023)

Tissue Engineering of cartilage has been hampered by the inability of engineered tissue to express native levels of type II collagen <i>in vitro</i>. Inadequate levels of type II collagen are, in part, due to a failure to recapitulate the physiological environment in culture. In this study, we engineered primary rabbit chondrocytes to express a secreted reporter, <i>Gaussia</i> Luciferase, driven by the type II collagen promoter, and applied a Design of Experiments approach to assess chondrogenic differentiation in micronutrient-supplemented medium. Using a Response Surface Model, 240 combinations of micronutrients absent in standard chondrogenic differentiation medium, were screened and assessed for type II collagen promoter-driven <i>Gaussia</i> luciferase expression. While the target of this study was to establish a combination of all micronutrients, alpha-linolenic acid, copper, cobalt, chromium, manganese, molybdenum, vitamins A, E, D and B7 were all found to have a significant effect on type II collagen promoter activity. Five conditions containing all micronutrients predicted to produce the greatest luciferase expression were selected for further study. Validation of these conditions in 3D aggregates identified an optimal condition for type II collagen promoter activity. Engineered cartilage grown in this condition, showed a 170% increase in type II collagen expression (Day 22 Luminescence) and in Young's tensile modulus compared to engineered cartilage in basal media alone.Collagen cross-linking analysis confirmed formation of type II-type II collagen and type II-type IX collagen cross-linked heteropolymeric fibrils, characteristic of mature native cartilage. Combining a Design of Experiments approach and secreted reporter cells in 3D aggregate culture enabled a high-throughput platform that can be used to identify more optimal physiological culture parameters for chondrogenesis.
Data from A Gene Expression Signature for Relapse of Primary Wilms Tumors (2023)

&lt;div&gt;Abstract&lt;p&gt;Anaplastic histology and metastasis are each associated with higher relapse and mortality rates in Wilms tumor patients. However, not all anaplastic tumors relapse and some nonanaplastic tumors relapse unexpectedly. To identify more accurate early prognostic indicators, we analyzed expression of 4,900 cancer-related genes in 26 primary Wilms tumors. This analysis revealed that expression of a set of four genes predicts future relapse of primary Wilms tumors with high accuracy, independent of anaplasia. Random permutation testing of this prognostic gene expression signature yielded &lt;i&gt;P&lt;/i&gt; = 0.003. Real-time reverse transcription-PCR analysis of the four genes in an independent primary tumor set resulted in correct prediction of future relapse with an accuracy of 92%. One of the four genes in the prognostic signature, CCAAT/enhancer binding protein β (&lt;i&gt;C/EBPB&lt;/i&gt;), is expressed at higher levels in both primary relapsing tumors and metastatic tumors than in primary nonrelapsing tumors. Short interfering RNA–mediated down-regulation of C/EBPB expression in WiT49, a cell line derived from a metastatic Wilms tumor, resulted in spontaneous apoptosis. These findings suggest that C/EBPB is a critical survival factor for Wilms tumor cells and that its expression contributes to the prognosis of Wilms tumor patients.&lt;/p&gt;&lt;/div&gt;
Corn Genotypic Variation Effects on Seedling Emergence and Leaf Appearance for Short‐Season Areas (2001)

Identifying corn ( Zea mays L.) genotypes with faster rates of seedling emergence and leaf appearance is important in developing a corn crop with earlier canopy closure and better seasonal light interception. A greenhouse experiment was conducted twice to investigate whether corn genotypes known to vary in canopy architecture and yield potential differed in rates of seedling emergence and leaf appearance. The experiment was arranged in a randomized complete block design utilizing seven genotypes: five of the newly developed Leafy reduced‐stature (LRS) types (LRS1, LRS2, LRS3, LRS4 and LRS5), one conventional type [Pioneer 3979 (P3979)], and one late‐maturing big‐leaf (LMBL) type. Five seeds were planted in each pot and seedling emergence was recorded every day until all seeds emerged. Leaf appearance was recorded from seedling emergence until the plants reached anthesis. There was variability among the genotypes for both seedling emergence and leaf appearance rate. Mean seedling emergence values of greater than 90 % were achieved by four of the five LRS genotypes, and the LRS types generally emerged more rapidly than the others. Leaf appearance rate showed linear increases over time; however, LRS genotypes (in particular LRS3, LRS4 and LRS5) had more rapid leaf appearance rates than the others. The LMBL hybrid ranked last for both seedling emergence (&lt;80 %) and leaf appearance rate. Rapid seedling emergence and leaf appearance by early‐maturing genotypes (LRS and P3979, especially LRS) may help these types of genotypes achieve earlier canopy closure and better use of the light available during the growing season, which is critical in a short growing season environment.
Seed Dormancy and Responses of Caryopses, Embryos, and Calli to Abscisic Acid in Wheat (1989)

Preharvest sprouting of wheat (Triticum aestivum L.) is associated with inadequate seed dormancy. Although abscisic acid (ABA) has often been suggested to play a central role in developing seed, its involvement in dormancy of mature seed lacks firm experimental evidence and endogenous ABA levels are not well correlated with germinability. We examined genotypic and temporal variation in wheat seed and embryo germination responses to ABA and determined whether differential sensitivity of embryos to ABA extended to growth of embryo-derived calli. Germination of Parker 76 caryopses, which have little dormancy at maturity, was only slightly inhibited by ABA, whereas germination of Clark's Cream, a highly dormant genotype, was greatly inhibited. Responsiveness of caryopses to ABA and dormancy of seeds decreased concurrently during afterripening. Germination of embryos excised from dormant and nondormant seeds was more responsive to ABA but otherwise was similar to that of caryopses, indicating that differential response to ABA occurs in the embryo. Growth of calli derived from immature embryos of two sprouting-susceptible wheat genotypes exceeded growth of calli from Clark's Cream, but no distinct differences in response to ABA among the genotypes were apparent. We concluded that the action of ABA is similar in developing and mature seeds, that genotypic and temporal variation in embryo responsiveness to endogenous ABA may be involved in dormancy, and that ABA probably acts in concert with other endogenous constituents.