gebhart/patrepeval huggingface dataset:
Immunization with inactivated whole virus particle influenza virus vaccines improves the humoral response landscape in cynomolgus macaques (2022)

Although antibody-inducing split virus vaccines (SV) are currently the most effective way to combat seasonal influenza, their efficacy can be modest, especially in immunologically-naïve individuals. We investigated immune responses towards inactivated whole influenza virus particle vaccine (WPV) formulations, predicated to be more immunogenic, in a non-human primate model, as an important step towards clinical testing in humans. Comprehensive analyses were used to capture 46 immune parameters to profile how WPV-induced responses differed to those elicited by antigenically-similar SV formulations. Naïve cynomolgus macaques vaccinated with either monovalent or quadrivalent WPV consistently induced stronger antibody responses and hemagglutination inhibition (HI) antibody titres against vaccine-matched viruses compared to SV formulations, while acute reactogenic effects were similar. Responses in WPV-primed animals were further increased by boosting with the same formulation, conversely to modest responses after priming and boosting with SV. 28-parameter multiplex bead array defined key antibody features and showed that while both WPV and SV induced elevated IgG responses against A/H1N1 nucleoprotein, only WPV increased IgG responses against A/H1N1 hemagglutinin (HA) and HA-Stem, and higher IgA responses to A/H1N1-HA after each vaccine dose. Antibodies to A/H1N1-HA and HA-Stem that could engage FcγR2a and FcγR3a were also present at higher levels after one dose of WPV compared to SV and remained elevated after the second dose. Furthermore, WPV-enhanced antibody responses were associated with higher frequencies of HA-specific B-cells and IFN-γ-producing CD4 + T-cell responses. Our data additionally demonstrate stronger boosting of HI titres by WPV following prior infection and support WPV administered as a priming dose irrespective of the follow up vaccine for the second dose. Our findings thus show that compared to SV vaccination, WPV-induced humoral responses are significantly increased in scope and magnitude, advocating WPV vaccination regimens for priming immunologically-naïve individuals and also in the event of a pandemic outbreak.
[Real-time monitoring method for ECG signals based on Kalman filter technique]. (2007)

In this paper, a prediction and real-time monitoring method for changing in ECG signals is proposed based on Kalman filter technique. It is important that the change point such as jump and drift could be predicted and diagnosed from the measurement, so as to keep it safely. The Kalman filter technique can be used in estimating system states in real-time way. The sudden changes of the estimation errors of system status can be used as the predictor for the physiologic changes. This method is validated in practice according to the experimental results.
The presence of chronic diseases contributes to the occurrence risk factors for gynecological cancers in Japan (2020)

The aim of the present study was to determine whether chronic diseases (CD), such as hypertension, diabetes mellitus, dyslipidemia, heart diseases and cerebrovascular diseases, are occurrence risk factors and affect the survival of patients with gynecological cancers (GC). The correlations between CD and the characteristics and survival of 1,590 GC patients [685 with cervical cancer (CC), 613 with endometrial cancer (EM) and 292 with ovarian cancer (OV)] were investigated in the present study. Of the CD patients, 189 had CC (27.6%), 265 had EM (43.2%) and 72 had OV (24.7%). The incidence of CD increased with age in GC patients. The number of CD patients aged ≥70 years, was 8.6-fold higher in the CC group, 3.0-fold higher in the EM group, and 9.6-fold higher in the OV group compared with those aged <50 years. CD and excess body weight were associated with GC regardless of patient age. However, there was no correlation between CD and survival at any age in GC patients. These findings indicate that CD contribute to >24% of the occurrence risk factors in GC patients in Japan.
The ATP binding cassette transporter A1 (ABCA1) modulates the development of aortic atherosclerosis in C57BL/6 and apoE-knockout mice (2001)

Identification of mutations in the ABCA1 transporter (ABCA1) as the genetic defect in Tangier disease has generated interest in modulating atherogenic risk by enhancing ABCA1 gene expression. To investigate the role of ABCA1 in atherogenesis, we analyzed diet-induced atherosclerosis in transgenic mice overexpressing human ABCA1 (hABCA1-Tg) and spontaneous lesion formation in hABCA1-Tg x apoE-knockout (KO) mice. Overexpression of hABCA1 in C57BL/6 mice resulted in a unique anti-atherogenic profile characterized by decreased plasma cholesterol (63%), cholesteryl ester (63%), free cholesterol (67%), non-high density lipoprotein (HDL)-cholesterol (53%), and apolipoprotein (apo) B (64%) but markedly increased HDL-cholesterol (2.8-fold), apoA-I (2.2-fold), and apoE (2.8-fold) levels. These beneficial changes in the lipid profile led to significantly lower (65%) aortic atherosclerosis in hABCA1-Tg mice. In marked contrast, ABCA1 overexpression had a minimal effect on the plasma lipid profile of apoE-KO mice and resulted in a 2- to 2.6-fold increase in aortic lesion area. These combined results indicate that overexpression of ABCA1 in C57BL/6 mice on a high cholesterol diet results in an atheroprotective lipoprotein profile and decreased atherosclerosis, and thus provide previously undocumented in vivo evidence of an anti-atherogenic role for the ABCA1 transporter. In contrast, overexpression of ABCA1 in an apoE-KO background led to increased atherosclerosis, further substantiating the important role of apoE in macrophage cholesterol metabolism and atherogenesis. In summary, these results establish that, in the presence of apoE, overexpression of ABCA1 modulates HDL as well as apoB-containing lipoprotein metabolism and reduces atherosclerosis in vivo, and indicate that pharmacological agents that will increase ABCA1 expression may reduce atherogenic risk in humans.
Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes (1987)

By using biotinylated enterotoxin DNA probes, a method to detect enterotoxigenic Escherichia coli by colony hybridization was developed. The treatment of colonies on nitrocellulose membrane filters with proteinase K and Triton X-100 was essential for obtaining the specific hybridization. A total of 200 E. coli strains isolated from travelers with diarrhea were tested for colony hybridization by using a probe encoding heat-labile toxin (LT) type h. All strains (86 of 86) that produced LT, but none of the non-LT producers, hybridized with 32P-labeled and biotinylated LT type h probes. A total of 36 strains chosen randomly from the 200 isolates were tested for colony hybridization by using heat-stable enterotoxin (ST) probes. All but two strains that hybridized with the 32P-labeled ST type Ia probe also hybridized with the biotinylated ST type Ia probe. All strains that hybridized with the 32P-labeled ST type Ib probe also hybridized with the biotinylated ST type Ib probe. Thus, almost all E. coli strains tested were judged to be the same by colony hybridization with biotinylated or 32P-labeled enterotoxin probes. These results demonstrate that the biotinylated enterotoxin probes are useful in the diagnosis of enterotoxigenic E. coli strains by colony hybridization.
Resultados imediatos e tardios do implante de stents farmacológicos para o tratamento de bifurcações: comparação entre as estratégias de um stent versus dois stents (2008)

FUNDAMENTOS: Os stents farmacológicos reduziram a necessidade de nova revascularização nas lesões bifurcadas. Entretanto, o seguimento tardio e a estratégia mais adequada (um stent vs. dois stents) não estão bem estabelecidos. MÉTODO: No período de junho de 2002 a dezembro de 2006, 156 pacientes consecutivos com lesões envolvendo bifurcações foram tratados com implante de stents farmacológicos. Comparou-se a taxa de eventos cardíacos adversos maiores (ECAM) combinados - óbito, infarto e revascularização do vaso-alvo (RVA) - e a incidência de trombose do stent conforme a estratégia (um stent: ramo principal vs. dois stents: ramos principal e lateral). RESULTADOS: A média das idades foi de 64 ± 12 anos, 75,6% eram do sexo masculino e 28,8%, diabéticos. O seguimento médio foi de 2,4 ± 1,3 anos. Utilizou-se a estratégia com um stent em 67,3% dos pacientes. Bifurcações verdadeiras ocorreram em 64,7% dos casos. A estratégia de dois stents foi adotada em 32,7% dos casos (crush: 14,7%; cullote: 3,2%; T-stent: 10,3%; simultaneous kissing stent: 3,8%; V-stent: 0,6%). Kissing balloon final foi utilizado em 85,9% dos casos. A sobrevida livre de ECAM estimada foi de 95% na estratégia com um stent vs. 86% com dois stents (teste do log-rank, p = 0,03). A análise multivariada identificou o uso de dois stents como único preditor independente de ECAM (OR: 2,82; IC 95%: 1,047,68; p = 0,04). A taxa de trombose no emprego de um stent em comparação com dois stents foi de 1,9% vs. 3,9% (p = 0,6). CONCLUSÕES: O emprego dos stents farmacológicos nas bifurcações apresenta baixas taxas de eventos combinados no seguimento tardio. O uso de dois stents associou-se a maior taxa de ECAM, embora constitua um subgrupo de maior complexidade.
Autocleaning filter used on air direct cooling system outdoor cabinet (2010)

Currently there are many different cooling methodologies used for outdoor cabinets; among them, the most used ones are the Heat Exchanger system (HXS) and the Air Direct Cooling System (ADCS). ADCS has the higher cooling efficiency, but on the other side it needs frequent maintenance given that sand, dust and dirt reduces the air flow passing through the filter. This study is focused on how to automatically clean up the filter, therefore strongly reducing the maintenance cost as well as the total OPEX.
Extraction of Low Molecular Weight Components from Fluoro-Polymers with Supercritical CO2 (2005)

フッ素系ポリマー中の低分子量成分は超臨界CO2によって抽出され, その抽出量は水をエントレーナーとして用いることで増加することが明らかとなった. 低分子量成分の抽出に影響を及ぼす因子としては, CO2密度, CO2流通量, 初期水分濃度があり, 特に, CO2の臨界密度付近以上で抽出が促進される傾向が見られた. 最適条件下では分子量が約1万以下の低分子量成分が抽出され, 精製したポリマーのMw/Mnを1.86から1.16まで下げることができた. 超臨界フルオロホルム, 超臨界フルオロエタンについても同様な抽出実験を試みたところ, 超臨界CO2が最も抽出効果が高いことがわかった.
Use of precipitated silica with silanol groups as an inorganic chain extender in polyurethane (2015)

In this study, novel polyurethane/silica (PU/SiO2) hybrid materials, prepared without an external crosslinking agent, were developed via the chemical reaction between urethane groups of PU prepolymer and hydroxyl groups at the surfaces of silica. The added inorganic filler-silica thus played the dual roles not only inorganic chain extender but also reinforcing agent in the preparation of the hybrid. Two different blending methods, were compared with respect to the mechanical properties of the PU/SiO2 hybrid materials: stirring mixing and three-roller shear mixing. The hybridization mechanism was confirmed using Fourier-transform infrared spectroscopy (FT-IR), solid-state 29Si NMR spectroscopy and X-ray diffraction (XRD). The dispersion of silica particles in the PU matrix was investigated by scanning electron microscopy (SEM). Because of the shear effect of three-roller shear process, the size of the silica aggregates tended to be more uniform. The tensile strength and elongation at break of the PU/SiO2 hybrid were 51 MPa and 590%, respectively, which represent increases of fourfold and 39% compared to those of neat PU. The thermogravimetric analysis (TGA) results for the PU/SiO2 hybrids indicated greater thermal stabilities and lower decomposition rates after hybridization. This work contributes new insights into the preparation of high-performance PU/SiO2 hybrids.
An Order-Preserving Encryption Scheme Based on Weighted Random Interval Division for Ciphertext Comparison in Wearable Systems (2022)

With the rapid development of wearable devices with various sensors, massive sensing data for health management have been generated. This causes a potential revolution in medical treatments, diagnosis, and prediction. However, due to the privacy risks of health data aggregation, data comparative analysis under privacy protection faces challenges. Order-preserving encryption is an effective scheme to achieve private data retrieval and comparison, but the existing order-preserving encryption algorithms are mainly aimed at either integer data or single characters. It is urgent to build a lightweight order-preserving encryption scheme that supports multiple types of data such as integer, floating number, and string. In view of the above problems, this paper proposes an order-preserving encryption scheme (WRID-OPES) based on weighted random interval division (WRID). WRID-OPES converts all kinds of data into hexadecimal number strings and calculates the frequency and weight of each hexadecimal number. The plaintext digital string is blocked and recombined, and each block is encrypted using WRID algorithm according to the weight of each hexadecimal digit. Our schemes can realize the order-preserving encryption of multiple types of data and achieve indistinguishability under ordered selection plaintext attack (IND-OCPA) security in static data sets. Security analysis and experiments show that our scheme can resist attacks using exhaustive methods and statistical methods and has linear encryption time and small ciphertext expansion rate.
Synthesis and characterization of novel biodegradable block copolymer poly(ethylene glycol)‐block‐poly(L‐lactide‐co‐2‐methyl‐2‐carboxyl‐propylene carbonate) (2005)

Abstract An amphiphilic block copolymer, poly(ethylene glycol)‐ block ‐poly( L ‐lactide‐ co ‐2‐methyl‐2‐benzoxycarbonyl‐propylene carbonate) [PEG‐ b ‐P(LA‐ co ‐MBC)], was synthesized in bulk by the ring‐opening polymerization of L ‐lactide with 2‐methyl‐2‐benzoxycarbonyl‐propylene carbonate (MBC) in the presence of poly(ethylene glycol) as a macroinitiator with diethyl zinc as a catalyst. The subsequent catalytic hydrogenation of PEG‐ b ‐P(LA‐ co ‐MBC) with palladium hydroxide on activated charcoal (20%) as a catalyst was carried out to obtain the corresponding linear copolymer poly(ethyleneglycol)‐block‐poly( L ‐lactide‐ co ‐2‐methyl‐2‐carboxyl‐propylenecarbonate) [PEG‐ b ‐P(LA‐ co ‐MCC)] with pendant carboxyl groups. DSC analysis indicated that the glass‐transition temperature ( T g ) of PEG‐ b ‐P(LA‐ co ‐MBC) decreased with increasing MBC content in the copolymer, and T g of PEG‐ b ‐P(LA‐ co ‐MCC) was higher than that of the corresponding PEG‐ b ‐P(LA‐ co ‐MBC). The in vitro degradation rate of PEG‐ b ‐P(LA‐ co ‐MCC) in the presence of proteinase K was faster than that of PEG‐ b ‐P(LA‐ co ‐MBC), and the cytotoxicity of PEG‐ b ‐P(LA‐ co ‐MCC) to chondrocytes from human fetal arthrosis was lower than that of poly( L ‐lactide). © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 4771–4780, 2005
Effect of polymer fibers recycled from waste tires on properties of wet-sprayed concrete (2018)

This study explores the possibility of using recycled tire polymer fibers (RTPF) as a micro-reinforcement in wet-sprayed concrete mixes. Two groups of mixes were made: sprayed concrete mixes with and without an air-entraining admixture. Each group comprised mixes with 0.9 and 1.8 kg/m3 of RTPF. To facilitate comparison, the groups contained either a plain mix, without fibers, or a mix with polypropylene (PP) fibers, usually used to control early-age cracking. The mixes were tested for their transport properties, including capillary absorption and gas permeability, freeze–thaw resistance, and autogenous and restrained deformation. Results show the beneficial effect of RTPF during freeze–thaw cycles and the deformation resistance of wet-sprayed mixes. Observed differences in the transport properties between mixes with and without air entrainment are explained by changes in pore structure, tested using mercury intrusion porosimetry (MIP).
A high-speed digital signal processor for atmospheric radar, part 7.3A (1984)

The Model SP-320 device is a monolithic realization of a complex general purpose signal processor, incorporating such features as a 32-bit ALU, a 16-bit x 16-bit combinatorial multiplier, and a 16-bit barrel shifter. The SP-320 is designed to operate as a slave processor to a host general purpose computer in applications such as coherent integration of a radar return signal in multiple ranges, or dedicated FFT processing. Presently available is an I/O module conforming to the Intel Multichannel interface standard; other I/O modules will be designed to meet specific user requirements. The main processor board includes input and output FIFO (First In First Out) memories, both with depths of 4096 W, to permit asynchronous operation between the source of data and the host computer. This design permits burst data rates in excess of 5 MW/s.
Lived experience of CamAPS FX closed loop system in youth with type 1 diabetes and their parents (2022)

Closed loop use via the CamAPS FX system was associated with modest improvements in aspects of the lived experience of managing type 1 diabetes in youth and their families. Further refinements of the system may optimize the user experience.
High-performance devices for a 0.15- mu m CMOS technology (1993)

Devices have been designed and fabricated in a CMOS technology with a nominal channel length of 0.15 mu m and minimum channel length below 0.1 mu m. In order to minimize short-channel effects (SCEs) down to channel lengths below 0.1 mu m, highly nonuniform channel dopings (obtained by indium and antimony channel implants) and shallow source-drain extensions/halo (by In and Sb preamorphization and low-energy As and BF/sub 2/ implant were used. Maximum high V/sub DS/ threshold rolloff was 250 mV at effective channel length of 0.06 mu m. For the minimum channel length of 0.1 mu m, the loaded (FI=FO=3, C=240 fF) and unloaded delays were 150 and 25 ps, respectively.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
PESSCARA: An Example Infrastructure for Big Data Research (2016)

Big data requires a flexible system for data management and curation which has to be intuitive, and it should also be able to execute non-linear analysis pipelines suitable to handle with the nature of big data. This is certainly true for medical images where the amount of data grows exponentially every year and the nature of images rapidly changes with technological advances and rapid genomic advances. In this chapter, we describe a system that provides flexible management for medical images plus a wide array of associated metadata, including clinical data, genomic data, and clinical trial information. The system consists of open-source Content Management System (CMS) that has a highly configurable workflow; has a single interface that can store, manage, enable curation, and retrieve imaging-based studies; and can handle the requirement for data auditing and project management. Furthermore, the system can be extended to interact with all the modern big data analysis technologies.
[Speech development in monosyllables of Chinese 3-years old children]. (2007)

It should be considered that monosyllables are different in difficulty to Chinese 3 years old children by oral imitation test when selecting materials and criteria of speech test for rehabilitation of deaf and dumb children.
Abstract C043: Addition of losartan to FOLFORINOX and chemoradiation downregulates pro-invasion and immunosuppression-associated genes in locally advanced pancreatic cancer (2022)

Abstract Purpose: Adding losartan to FOLFIRINOX (FFX) chemotherapy followed by chemoradiation (CRT) resulted in 61% R0 surgical resection in our phase II trial in patients with locally advanced pancreatic cancer. Here we identify potential mechanisms of benefit by assessing the effects of neoadjuvant losartan+FFX+CRT versus FFX+CRT on the stromal tumor microenvironment. Experimental Design: We performed a gene expression analysis of RNA extracted from pancreatic cancer tissue sections and immunofluorescence for cancer cells and immune cells using archived surgical samples from patients treated with losartan+FFX+CRT (NCT01591733), FFX+CRT (NCT01591733) or surgery upfront, without any neoadjuvant therapy. We then assessed whether certain gene sets could stratify the overall survival of patients. Results: Neoadjuvant losartan+FFX+CRT and FFX+CRT increased the expression of genes linked to vascular normalization, transendothelial migration of leukocytes, T cell activation and cytolytic activity, and dendritic cell related genes versus no neoadjuvant treatment. In comparison to FFX+CRT, losartan+FFX+CRT downregulated pro-invasion, immunosuppression, and M2 macrophages related genes, and upregulated genes associated with tumor suppression, including the p53 pathway. Furthermore, immunostaining revealed significantly less residual disease in lesions treated with losartan+FFX+CRT versus FFX+CRT. Losartan+FFX+CRT also reduced CD4+FOXP3+ regulatory T cells in pancreatic cancer lesions with a complete/near complete response. Overall survival was associated with dendritic cell and antigen presentation genes for patients treated with FFX+CRT, and with immunosuppression and invasion genes or dendritic cell- and blood vessel-related genes for those treated with losartan+FFX+CRT. Conclusions: Adding losartan to FFX+CRT reduced pro-invasion and immunosuppression related genes, which were associated with improved treatment outcomes in patients with locally advanced pancreatic cancer. Citation Format: Yves Boucher, Jessica M. Posada, Sonu Subudhi, Spencer R. Rosario, Liqun Gu, Ashwin S. Kumar, Heena Kumra, Mari Mino-Kenudson, Nilesh P. Talele, Dan G. Duda, Dai Fukumura, Jennifer Y. Wo, Jeffrey W. Clark, David P. Ryan, Carlos Fernandez-Del Castillo, Theodore S. Hong, Mikael J. Pittet, Rakesh K. Jain. Addition of losartan to FOLFORINOX and chemoradiation downregulates pro-invasion and immunosuppression-associated genes in locally advanced pancreatic cancer [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer; 2022 Sep 13-16; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2022;82(22 Suppl):Abstract nr C043.
The Introduction of Proteins into Mammalian Cells by Electroporation (2003)

Proteins can be introduced into cells in several ways, including permeabilization by Sendai virus, trypsinization, osmotic shock, microinjection, electroporation, or after transfection of cells with expression vectors containing the gene(s) of interest. Unlike most of these other methods, electroporation is a simple, relatively inexpensive, and efficient method for rapidly introducing DNA, small molecules, and proteins into bacterial or mammalian cells (1–10). Electroporation has the advantage over other techniques in that many cells can be treated simultaneously and one or more compounds or macromolecules, such as proteins, can be introduced into cells at the same time (see Note 1).