gebhart/patrepeval huggingface dataset:
Interactions of a copper antitumor drug with DNA: [1-(.alpha.-pyridylmethylene)-2-(.alpha.-pyridyl)hydrazine]copper(II) chloride does not intercalate (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteractions of a copper antitumor drug with DNA: [1-(.alpha.-pyridylmethylene)-2-(.alpha.-pyridyl)hydrazine]copper(II) chloride does not intercalateDebra L. Banville, W. David Wilson, and Luigi G. MarzilliCite this: Inorg. Chem. 1985, 24, 16, 2479–2483Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/ic00210a005https://doi.org/10.1021/ic00210a005research-articleACS PublicationsRequest reuse permissionsArticle Views128Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Role of 7,8-dihydroneopterin in T-cell apoptosis and HTLV-1 transcription in vitro. (1996)

Adult T-cell leukemia is associated with high levels of neopterin, released in large amounts from human macrophages upon stimulation with interferon-gamma. Recent data suggested a potential role of neopterin-derivatives in oxygen radical-mediated processes, and evidence accumulates that oxidative stress is involved in the pathogenesis of viral diseases. We now report that increased concentrations of 7,8-dihydroneopterin may lead to enhanced apoptosis and disturbance of the redox-balance of human leukemic Jurkat T cells. Additionally, we demonstrate that 7,8-dihydroneopterin and hydrogen peroxide activate the type 1 human T-cell leukemia virus (HTLV-1) long terminal repeat (LTR). Furthermore, we found that the activity of the HTLV-1 transactivator protein Tax is amplified by an elevated concentration of 7,8-dihydroneopterin. Tax did not significantly augment 7,8-dihydroneopterin mediated apoptosis. Based on our data we propose that 7,8-dihydroneopterin may be involved in the progression to higher stages of HTLV-1 associated disease.
Cell fate choices and the expression of Notch, Delta and Serrate homologues in the chick inner ear: parallels with Drosophila sense-organ development (1998)

The sensory patches in the vertebrate inner ear are similar in function to the mechanosensory bristles of a fly, and consist of a similar set of cell types. If they are truly homologous structures, they should also develop by similar mechanisms. We examine the genesis of the neurons, hair cells and supporting cells that form the sensory patches in the inner ear of the chick. These all arise from the otic epithelium, and are produced normally even in otic epithelium cultured in isolation, confirming that their production is governed by mechanisms intrinsic to the epithelium. First, the neuronal sublineage becomes separate from the epithelial: between E2 and E3.5, neuroblasts delaminate from the otocyst. The neuroblasts then give rise to a mixture of neurons and neuroblasts, while the sensory epithelial cells diversify to form a mixture of hair cells and supporting cells. The epithelial patches where this occurs are marked from an early stage by uniform and maintained expression of the Notch ligand Serrate1. The Notch ligand Delta1 is also expressed, but transiently and in scattered cells: it is seen both early, during neuroblast segregation, where it appears to be in the nascent neuroblasts, and again later, in the ganglion and in differentiating sensory patches, where it appears to be in the nascent hair cells, disappearing as they mature. Delta-Notch-mediated lateral inhibition may thus act at each developmental branchpoint to drive neighbouring cells along different developmental pathways. Our findings indicate that the sensory patches of the vertebrate inner ear and the sensory bristles of a fly are generated by minor variations of the same basic developmental program, in which cell diversification driven by Delta-Notch and/or Serrate-Notch signalling plays a central part.
Sources of unreliability and bias in standardized‐patient rating (1991)

In tests of clinical competence, standardized patients (SPs) can be used to present the clinical problem and rate actions taken by the examinee in the patient encounter. Both these aspects of the “test”; have the potential to contribute to unreliability and bias in measurement. In 1987, two universities collaborated to develop and execute the same SP test to clinical clerks in their respective institutions. This provided us with the opportunity to evaluate rating bias attributable to test site and three sources of rating unreliability within the same population of raters: those attributable to inconsistencies within the same rater (within‐rater reliability), those attributable to inconsistencies between two raters trained in the same test site (between‐raters reliability—same site), and those attributable to inconsistencies between two raters trained in different test sites (between‐raters reliability—different sites). A stratified random sample of 537 of the 2,560 examinee‐patient encounters that occurred in the inter‐university examination was videotaped, providing equivalent representation of the 16 cases used in the test and the two universities. Videotaped encounters from both universities were rated by 44 SPs who presented and rated the case during the examination. Videotape and examination ratings were used to estimate systematic rating bias and the three types of rater reliability. Overall, rater reliability of individual items and overall encounter score were fair to good (.37 to .52). Consistent with these results, raters within cases accounted for 20% of the observed variance in student scores. Within‐rater reliability was better than both types of between‐raters reliability. Rater agreement was not influenced by test site, but systematic differences in score were present between test sites. Site 1 raters scored the same students, on average, 6.7% lower than Site 2 raters. These differences had an impact on the proportion of students who would have failed the check list portion of the test. In Site 1,50% of the students rated had data‐collection scores below 60%, whereas, inSite2, only 33% had scores below the 60% cutoff. The implications of these findings for single‐ and multi‐site SP‐based tests of competence are explored, and additional areas for research are identified.
Acute atrial arrhythmogenesis in murine hearts following enhanced extracellular Ca2+entry depends on intracellular Ca2+stores (2009)

Enhanced extracellular Ca(2+) entry exerts acute atrial arrhythmogenic effects that is nevertheless dependent upon diastolic Ca(2+) release. These findings complement reports that associate established, chronic, atrial arrhythmogenesis with decreased overall inward Ca(2+) current.
Interplay between carbohydrate and lipid in recognition of glycolipid antigens by natural killer T cells (2012)

Natural killer T (NKT) cells are a T cell subpopulation that were named originally based on coexpression of receptors found on natural killer (NK) cells, cells of the innate immune system, and by T lymphocytes. The maturation and activation of NKT cells requires presentation of glycolipid antigens by CD1d, a cell surface protein distantly related to the major histocompatibility complex (MHC)-encoded antigen presenting molecules. This specificity distinguishes NKT cells from most CD4(+) and CD8(+) T cells that recognize peptides presented by MHC class I and class II molecules. The rapid secretion of a large amount of both Th1 and Th2 cytokines by activated NKT cells endows them with the ability to play a vital role in the host immune defense against various microbial infections. In this review, we summarize progress on identifying the sources of microbe-derived glycolipid antigens recognized by NKT cells and the biochemical basis for their recognition.
The potential role of feature tracking in adult congenital heart disease: advantages and disadvantages in measuring myocardial deformation by cardiovascular magnetic resonance (2018)

Cardiac magnetic resonance (CMR) imaging has several advantages over other imaging modalities in adult patients with congenital heart disease. Limitations remain however, in the assessment of myocardial function based on volumetric methods. There is increasing evidence in a range of diseases that myocardial strain is a more sensitive measure of regional and global ventricular contractile function. Feature tracking (FT), is a CMR-based method that measures strain without the need for additional sequence acquisition which can be analyzed rapidly, opening the possibility of use in adult congenital heart disease (ACHD). The aim of this review is to outline the technique of FT-CMR, its advantages and disadvantages compared to other techniques, review the values obtained, and to outline the preliminary data in support of its use in ACHD. In short, FT is a semi-automated post-processing technique that can be performed on routinely acquired images and thereby does not prolong scan time, is reproducible and not overly time-consuming. Although FT-CMR promises much, inevitably there remain limitations. While FT has excellent spatial resolution, temporal resolution is sub-optimal relative to speckle tracking echocardiography. FT-CMR is an emerging technique of measuring the myocardial strain that is comparable to speckle tracking echocardiography and the gold standard myocardial tagging CMR. FT-CMR derived strain parameters have potential diagnostic, risk stratification and prognostic value in the growing ACHD population.
Emerging ideas for innovation in Indigenous education: a research synthesis of Indigenous educative roles in mainstream and flexi schools (2017)

The Indigenous education agenda in Australia remains focused on mainstream schooling contexts. Although overlooked in Indigenous education discourse, flexi schools appear to be engaging with disproportionately high numbers of Indigenous students and staff. The educative roles of Indigenous peoples in broader Indigenous education discourse are relatively underexplored. Even less is known about Indigenous educative roles in flexi schools. This paper presents a research synthesis of Indigenous staff roles in mainstream and flexi schools. The literature paints a contrasting picture between the two schooling approaches. The disparity in Indigenous student and staff numbers in mainstream and flexi schools offers new ideas to the broader Indigenous education agenda that may have implications for all education settings.
Increased Level of Long Non-Coding RNA MALAT1 Is a Common Feature of Amoeboid Invasion (2020)

The ability of cancer cells to adopt various migration modes (the plasticity of cancer cell invasiveness) is a substantive obstacle in the treatment of metastasis, yet still an incompletely understood process. We performed a comparison of publicly available transcriptomic datasets from various cell types undergoing a switch between the mesenchymal and amoeboid migration modes. Strikingly, lncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) was one of three genes that were found upregulated in all amoeboid cells analyzed. Accordingly, downregulation of MALAT1 in predominantly amoeboid cell lines A375m2 and A2058 resulted in decrease of active RhoA (Ras homolog family member A) and was accompanied by the amoeboid-mesenchymal transition in A375m2 cells. Moreover, MALAT1 downregulation in amoeboid cells led to increased cell proliferation. Our work is the first to address the role of MALAT1 in MAT/AMT (mesenchymal to amoeboid transition/amoeboid to mesenchymal transition) and suggests that increased MALAT1 expression is a common feature of amoeboid cells.
Application of genetics and genomics in drug development (2000)

Pharmacogenetics is capitalizing on the tools of the Human Genome Project to introduce the new genomic information into drug development. The applications vary from identifying individuals at risk for a disease, to developing gene signatures of drug efficacy and adverse effects. Pharmacogenetics is a field in evolution from which significant growth is expected in the years to come. Drug Dev. Res. 49:43–45, 2000. © 2000 Wiley-Liss, Inc.
Continuous monitoring of stress on smartphone using heart rate variability (2015)

Continuous monitoring of an individual's stress levels is essential to manage stress and mental state in an effective way. With increasing ubiquity of wearable heart rate monitors and their unobtrusiveness, HRV (Heart rate variability) derived from heart rate signals has emerged as one of the most relevant parameters for continuous monitoring of stress. In the present work, we have made an attempt to address the challenges about distinguishing between stressed and non-stressed state of a person based on just one minute of IBI (Inter Beat Interval) records with good accuracy. Such ultra-short term analysis of HRV is particularly advantageous towards capturing very short term fluctuations in mental stress levels and enhanced scope for frequent monitoring. We have analyzed various time domain, frequency domain and nonlinear HRV features to narrow down to a most influential set of features for accurate classification between stressed and non-stressed state. We have identified RMSSD (root mean square of successive differences) of IBI series to be the most direct indicator of stressed state. We also provide a continuous stress score which, when used in continuous monitoring scenario, provides the user with adequate details about his/her stress levels. This helps the user to understand stress patterns across a day in a better way and to take appropriate measures to manage stressful situations. We have developed and deployed a system, based on above concept, on smartphone as an android application for real-time stress monitoring.
Epidermal Growth Factor Receptor Inhibition Induces Trichomegaly (2003)

(2003). Epidermal Growth Factor Receptor Inhibition Induces Trichomegaly. Acta Oncologica: Vol. 42, No. 4, pp. 345-346.
Advancing the understanding of the embryo transcriptome co-regulation using meta-, functional, and gene network analysis tools (2008)

Embryo development is a complex process orchestrated by hundreds of genes and influenced by multiple environmental factors. We demonstrate the application of simple and effective meta-study and gene network analyses strategies to characterize the co-regulation of the embryo transcriptome in a systems biology framework. A meta-analysis of nine microarray experiments aimed at characterizing the effect of agents potentially harmful to mouse embryos improved the ability to accurately characterize gene co-expression patterns compared with traditional within-study approaches. Simple overlap of significant gene lists may result in under-identification of genes differentially expressed. Sample-level meta-analysis techniques are recommended when common treatment levels or samples are present in more than one study. Otherwise, study-level meta-analysis of standardized estimates provided information on the significance and direction of the differential expression. Cell communication pathways were highly represented among the genes differentially expressed across studies. Mixture and dependence Bayesian network approaches were able to reconstruct embryo-specific interactions among genes in the adherens junction, axon guidance, and actin cytoskeleton pathways. Gene networks inferred by both approaches were mostly consistent with minor differences due to the complementary nature of the methodologies. The top-down approach used to characterize gene networks can offer insights into the mechanisms by which the conditions studied influence gene expression. Our work illustrates that further examination of gene expression information from microarray studies including meta- and gene network analyses can help characterize transcript co-regulation and identify biomarkers for the reproductive and embryonic processes under a wide range of conditions.
Optimization of multi-element LED source for uniform illumination of plane surface (2011)

In many lighting applications at the lux level, full coverage of the target surface, uniformity, and glare are important requirements. In the case of LEDs, luminaire optical output depends upon the source of luminous flux, radiation spatial distribution, geometry of the LED array, and source-to-target distance. One has to optimize these parameters to fulfill the needs of the application and to have an efficient LED luminaire. This paper discusses the interdependencies of these parameters. The optimization of a ring LED source configuration is demonstrated for an application of uniform illumination of a plane surface with minimum source flux and with no glare.
Cooperativity from electrostatic interactions: understanding bistability in molecular crystals (2009)

A general model for explaining bistability in crystals of donor–acceptor (DA) molecules, associated with an intramolecular charge-transfer process, is thoroughly discussed. Specifically, an extension from the two- to the three-essential states model for D-bridge-A molecular units taking into account the active role of the bridge states in the charge-transfer process is proposed. We demonstrate that the phenomenon of bistability is so robust that it survives when such an extended model is adopted. Finally, we discuss the molecular and supramolecular requirements for the observation of bistability phenomena in molecular crystals.
Influence of water‐miscible organic solvents on kinetics and enantioselectivity of the (R,‐specific alcohol dehydrogenase from Lactobacillus brevis (2006)

Using the organic solvents acetonitrile and 1,4-dioxane as water-miscible additives for the alcohol dehydrogenase (ADH)-catalyzed reduction of butan-2-one, we investigated the influence of the solvents on enzyme reaction behavior and enantioselectivity. The NADP(+)-dependent (R)-selective ADH from Lactobacillus brevis (ADH-LB) was chosen as biocatalyst. For cofactor regeneration, the substrate-coupled approach using propan-2-ol as co-substrate was applied. Acetonitrile and 1,4-dioxane were tested from mole fraction 0.015 up to 0.1. Initial rate experiments revealed a complex kinetic behavior with enzyme activation caused by the substrate butan-2-one, and increasing K(M) values with increasing solvent concentration. Furthermore, these experiments showed an enhancement of the enantioselectivity for (R)-butan-2-ol from 37% enantiomeric excess (ee) in pure phosphate buffer up to 43% ee in the presence of 0.1 mol fraction acetonitrile. Finally, the influence of the co-solvents on water activity of the reaction mixture and on enzyme stability was investigated.
Postnatal reduction of tuberous sclerosis complex 1 expression in astrocytes and neurons causes seizures in an age‐dependent manner (2017)

These findings support a model in which the developmental timing of TSC1 loss dictates the severity of neuronal and glial abnormalities and resulting epilepsy.
Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion (2002)

Secretion of platelet granules is necessary for normal hemostasis. Platelet secretion requires soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complex formation between different members of the syntaxin, SNAP-25, and vesicle-associated membrane protein (VAMP) gene families. Using microcapillary reverse-phase high-performance liquid chromatography-nano-electrospray tandem mass spectrometry, we identified VAMP-3 and VAMP-8 as VAMP isoforms coimmunoprecipitated from platelets with syntaxin 4. Immunoblotting experiments confirmed the presence of VAMP-3 and VAMP-8 but not VAMP-1 or VAMP-2 in platelets. To examine the effect of VAMP proteins on platelet secretion, soluble recombinant (r) VAMP-2, rVAMP-3, and rVAMP-8 were incubated with streptolysin O-permeabilized platelets. Secretion of alpha granules (monitored by flow cytometric measurement of P-selectin) was blocked, and dense-granule secretion (assessed by release of carbon 14-serotonin) was almost completely inhibited by rVAMP-3, whereas rVAMP-8 inhibited secretion of dense granules but not alpha granules. In contrast, rVAMP-2, which formed SNARE complexes in vitro, had no effect on platelet exocytosis. We conclude that VAMP-3 and VAMP-8 form SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion.
Phthalhydrazide Chemiluminescence Method for Determination of Hydroxyl Radical Production: Modifications and Adaptations for Use in Natural Systems (2010)

The hydroxyl radical is formed through a variety of processes pertinent to natural and anthropogenic systems. Here we report development of a simple and sensitive trap-and-trigger chemiluminescence method based upon the hydroxylation of phthalhydrazide to 5-hydroxy-2,3-dihydro-1,4-phthalazinedione, which emits chemiluminescence when oxidized under alkaline conditions. Cu(III) is employed as an oxidant and is shown to be relatively insensitive to a range of interferences likely to be encountered. The method has been standardized by use of γ-radiolysis of water as a primary source of hydroxyl radical, with a convenient secondary calibration procedure developed that uses Fenton chemistry. Detection limits varied from 7.4 nM (at pH 3) and 6.2 nM (at pH 8.1) of accumulated hydroxyl radical production in a simple 10 mM NaCl matrix to around 30 nM in an artificial seawater medium, due to competition for hydroxyl between the phthalhydrazide probe and bromide. The method has been used to characterize the kinetics of the Fenton system employed for calibration and is shown to be consistent with published models of this process over time scales of several hours. The application of this method to a range of matrices and for photochemical studies is also described.
Australia's Botanic Gardens Weed Risk Assessment Procedure (2008)

The majority of agricultural and environmental weeds in Australia have originated from ornamental horticulture. Botanic gardens require a simple yet robust method for rapidly screening the relative weed risk of taxa in their collections. A ten question, multiple choice, additive weed risk assessment system was developed for application to taxa growing in gardens. The major city botanic gardens in Melbourne, Hobart and Perth used the system to score 100 taxa. Separately, a national survey and a literature review of the same 100 taxa were undertaken to obtain two independent datasets for assessing their level of weediness. Testing of the system showed that it has good discriminatory power and cut-off scores were chosen that have about 80% accuracy for both low weed risk and high weed risk species. There was poorer accuracy to distinguish medium weed risk species. The system has wider potential application for the nursery and garden industry as a tool to help reduce the number of high weed risk ornamental species in cultivation.