gebhart/patrepeval huggingface dataset:
MLL is involved in a t(2;11)(p21;q23) in a patient with acute myeloblastic leukemia (1999)

We describe a patient with acute myeloblastic leukemia (AML-M0) whose cells had a t(2;11)(p21;q23). Fluorescence in situ hybridization analysis with a probe for MLL showed that it was split, hybridizing to both the derivative 2 and 11 chromosomes. Nineteen other patients with 2p;11q translocations have been described; breakpoints in 14 of these are the same as in the case we describe. The phenotype of these patients is quite variable, with 14 patients having myelodysplastic syndrome which evolved to AML in six. Four patients had AML and two had acute lymphoblastic leukemia. MLL status has been studied in two other patients; one had MLL rearranged and one did not.
Spatial Light Modulators for Maskless Lithography (2006)

We present a piston-type SLM (spatial light modulator) as a programmable pattern generator for maskless lithography. Large arrays of up to ~40,000 mirrors with 20 mum pixels have been fabricated. The mirrors have high resonance frequencies (f=564 kHz), and achieve deflections of lambda/2 for actuation voltages <100 V. Using the SLM in a simple imaging system, we demonstrate the generation of aerial images of dark line patterns
Activity of Maturation Promoting Factor (MPF) and Mitogen-Activated Protein Kinase (MAPK) after Parthenogenetic Activation of Ovine Oocytes (2000)

The maturation promoting factor (MPF) and mitogen-activated protein kinase (MAPK) are the key regulators of both meiotic and mitotic cell cycles. Knowledge of the dynamics of these two kinases during the transition from meiosis to mitosis would be of great importance for cloning by nuclear transfer. In this study, experiments were designed to assay the changes of MPF and MAP kinase activity of in vitro matured ovine oocytes after chemical activation and culture in 0 mM or 2 mM 6-dimethylaminopurine (6-DMAP) for 12 h. Moreover, to determine the biological significance of the fluctuations of MPF, activated oocytes were fused with GV-staged partners. The biochemical results showed that the high MPF activity of MII oocytes fell to basal level precipitously within the first hour after activation, started to increase at 6-8 h, rising to 80 +/- 4% of MII after 12 h. MAPK activity decreased to a low level 4 h after activation, increased between 6-12 h, but remained below 30 +/- 3.6% of MII values. The incubation with 6-DMAP had no effect on the kinetics of MPF and MAP kinase activity. Fusion of MII oocytes to GV partners induced rapid breakdown of the GV, whereas no breakdown occurred when GV were fused with eggs in the first hours post activation. Interestingly, the high biochemical levels of MPF activity at 8-12 h after activation were not able to induce GVBD in fusion partners.
Simulation of bubbles in foam with the volume control method (2007)

Liquid and gas interactions often produce bubbles that stay for a long time without bursting on the surface, making a dry foam structure. Such long lasting bubbles simulated by the level set method can suffer from a small but steady volume error that accumulates to a visible amount of volume change. We propose to address this problem by using the volume control method. We track the volume change of each connected region, and apply a carefully computed divergence that compensates undesired volume changes. To compute the divergence, we construct a mathematical model of the volume change, choose control strategies that regulate the modeled volume error, and establish methods to compute the control gains that provide robust and fast reduction of the volume error, and (if desired) the control of how the volume changes over time.
[The detection of rotavirus RNA in nasopharyngeal smears by molecular hybridization]. (1991)

Rotavirus RNA was detected in nasopharyngeal washings, obtained from patients with rotavirus gastroenteritis (76.3% of cases) and gastroenteritis of unknown etiology (34.7% of cases), by the method of molecular hybridization with the use of RNA of monkey virus SA11, labeled with 32P in the process of T4-ligase reaction. In 100% of cases this detection correlated with the presence of lesions of the upper respiratory ways and was probably indicative of the rotavirus nature of the catarrhal syndrome in rotavirus gastroenteritis.
Ophthalmic pathology with clinical correlations (1997)

The pathologist and clinician skin and conjunctiva inflammation - granulomatous and nongranulomatous paediatric ophthalmology, including genetics and retinoblastoma cornea and sclera lens glaucoma diabetes trauma and wound healing uvea (including melanoma) retina optic nerve orbit and lacrimal system.
Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice (2020)

In the heart, the serine carboxypeptidase cathepsin A (CatA) is distributed between lysosomes and the extracellular matrix (ECM). CatA-mediated degradation of extracellular peptides may contribute to ECM remodeling and left ventricular (LV) dysfunction. Here, we aimed to evaluate the effects of CatA overexpression on LV remodeling. A proteomic analysis of the secretome of adult mouse cardiac fibroblasts upon digestion by CatA identified the extracellular antioxidant enzyme superoxide dismutase (EC-SOD) as a novel substrate of CatA, which decreased EC-SOD abundance 5-fold. <i>In vitro</i>, both cardiomyocytes and cardiac fibroblasts expressed and secreted CatA protein, and only cardiac fibroblasts expressed and secreted EC-SOD protein. Cardiomyocyte-specific CatA overexpression and increased CatA activity in the LV of transgenic mice (CatA-TG) reduced EC-SOD protein levels by 43%. Loss of EC-SOD-mediated antioxidative activity resulted in significant accumulation of superoxide radicals (WT, 4.54 μmol/mg tissue/min; CatA-TG, 8.62 μmol/mg tissue/min), increased inflammation, myocyte hypertrophy (WT, 19.8 μm; CatA-TG, 21.9 μm), cellular apoptosis, and elevated mRNA expression of hypertrophy-related and profibrotic marker genes, without affecting intracellular detoxifying proteins. In CatA-TG mice, LV interstitial fibrosis formation was enhanced by 19%, and the type I/type III collagen ratio was shifted toward higher abundance of collagen I fibers. Cardiac remodeling in CatA-TG was accompanied by an increased LV weight/body weight ratio and LV end diastolic volume (WT, 50.8 μl; CatA-TG, 61.9 μl). In conclusion, CatA-mediated EC-SOD reduction in the heart contributes to increased oxidative stress, myocyte hypertrophy, ECM remodeling, and inflammation, implicating CatA as a potential therapeutic target to prevent ventricular remodeling.
Supplementary Table from Plasma Metabolomics Analysis of Aspirin Treatment and Risk of Colorectal Adenomas (2023)

Supplementary Table from Plasma Metabolomics Analysis of Aspirin Treatment and Risk of Colorectal Adenomas
Effect of clindamycin hydrochloride on oral malodor, plaque, calculus, and gingivitis in dogs with periodontitis. (2000)

Periodontal disease is considered the most common disease of dogs and cats. The clinical sign most frequently reported by clients is oral malodor. Clindamycin hydrochloride has been used for several years for the treatment of periodontal disease in both cats and dogs. This study was designed to assess the effect of clindamycin HCl when used in two different post-prophylaxis treatment regimens on oral malodor and periodontal disease in a controlled clinical trial. This study demonstrated that a 5-day postprophylaxis regimen was significantly effective in reducing oral malodor compared with a dental prophylaxis alone. Clindamycin HCl significantly reduced oral malodor from the animals' baseline levels through 42 days. In addition, although no effect was observed on periodontal pocket depth, this treatment regimen also resulted in significant reductions in dental plaque, dental calculus, and gingival bleeding.
Quantum confinement-tunable intersystem crossing and the triplet state lifetime of cationic porphyrin–CdTe quantum dot nano-assemblies (2015)

Here, we report a ground-state interaction between the positively charged cationic porphyrin and the negatively charged carboxylate groups of the thiol ligands on the surface of CdTe quantum dots (QDs), leading to the formation of a stable nanoassembly between the two components. Our time-resolved data clearly demonstrate that we can dramatically tune the intersystem crossing (ISC) and the triplet state lifetime of porphyrin by changing the size of the QDs in the nanoassembly.
Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems (2014)

The here reported phenomena refer to typical experiments in biotechnological labs. For this reason these aspects are highlighted in this work to make operators of such valuable techniques as the BioLector aware for potential pitfalls and resulting misinterpretations. With the right approach it is possible to minimize existing problems and deal with them.
Modification of recombinant asparaginase from Erwinia carotovora with polyethylene glycol 5000 (2007)

A method for polyethylene conjugation with recombinant asparaginase has been developed to improve therapeutically important properties of this enzyme. Methoxy-nitrophenyl carbamate of polyethylene glycol with molecular weight 5000 was employed as the modification reagent. Optimization of pegylation procedure allowed achieving high level of the enzyme modification. Under 4.5 molar excess of modification reagent more than 10 molecules of methoxy-polyethylene bound per one asparaginase molecular. The modified asparaginase retained 57% of initial activity. A simple and efficient pegylation procedure described in this work can be used for asparaginase production with improved therapeutic properties.
GaN nanotip pyramids formed by anisotropic etching (2003)

We experimentally demonstrate the formation of GaN nanotip pyramids by selective and anisotropic etching of N-polar GaN in KOH solution. For samples grown with adjacent Ga- and N-polar regions on the same wafer, the KOH solution was found to selectively etch only the N-polar surface while leaving the Ga-polar surface intact. An aggregation of hexagonal pyramids with well defined {10 1̄ 1̄} facets and very sharp tips with diameters less than ∼20 nm were formed. The density of the pyramids can be controlled by varying the KOH concentration, solution temperature or the etch duration. The GaN etching activation energy is estimated to be Ea≈0.587 eV. Dense GaN pyramids with sharp tips have applications in both electronic and photonic devices.
Extraction and Plastein Reaction of Bioactive Peptides from Agaricus Bisporus Mushrooms (2019)

The common mushroom <em>Agaricus bisporus</em> has a high content. [...]
Synthesis of Enantioenriched α‐(Hydroxyalkyl)‐tri‐n‐butylstannanes (2008)

Li und Mg – gewogen und zu leicht befunden: Die katalytische asymmetrische Synthese der Titelverbindungen in Form der Ester oder Thiocarbamate 1 gelingt mit guten Ausbeuten und Enantioselektivitäten, wenn eine äquimolare Menge an nBu3SnH und Et2Zn in Dimethoxyethan als Reagens eingesetzt wird (siehe Schema; PG=Schutzgruppe, L*=chiraler Ligand). Lithium- oder Magnesiumderivate ergaben dagegen keine Enantioselektivität. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2008/z802313_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Observation of Large Photoacoustic Signal Phase Changes during a Diffusion Process (2005)

The phase of the photoacoustic signal is known to be a sensitive and accurate means to investigate, both qualitatively and quantitatively, static multilayer heterogeneous systems. According to theory, the maximum phase delay for a very weakly absorbing homogeneous sample should be within 45 degrees of a very strongly absorbing sample, while for heterogeneous samples the phase delay can be greater than 45 degrees. Here we report the observation of photoacoustic phase delays greater than 350 degrees by extending the use of step-scan phase modulation photoacoustic spectroscopy to study a non-repetitive dynamic system in situ, in real time. These large phase delays correspond to sampling several thermal diffusion lengths into the sample. The model system used in this study consisted of a hydrocarbon grease diffusing through a porous Teflon film. The progress of the diffusion was tracked by monitoring both the photoacoustic signal magnitude and the phase of the hydrocarbon grease after isolation from the Teflon film signal contributions at two different phase modulation frequencies.
Fabrication of a Low-Cost, Fiber-Coupled, and Air-Spaced Fabry-P&#233;rot Etalon (2023)

Fabry-Pérot etalons (FPE) have found their way into many applications. In fields such as spectroscopy, telecommunications, and astronomy, FPEs are used for their high sensitivity as well as their exceptional filtering capability. However, air-spaced etalons with high finesse are usually built by specialized facilities. Their production requires a clean room, special glass handling, and coating machinery, meaning commercially available FPEs are sold for a high price. In this article, a new and cost-effective method to fabricate fiber-coupled FPEs with standard photonic laboratory equipment is presented. The protocol should serve as a step-by-step guide for the construction and characterization of these FPEs. We hope this will enable researchers to conduct fast and cost-effective prototyping of FPEs for various fields of application. The FPE, as presented here, is used for spectroscopic applications. As shown in the representative results section via proof of principle measurements of water vapor in ambient air, this FPE has a finesse of 15, which is sufficient for the photothermal detection of trace concentrations of gases.
Optical recordings of cardiac transmembrane potential around an extracellular electrode in electrically anisotropic, bullfrog atrium (2002)

The authors have studied the spatial distribution of cardiac transmembrane potential around an extracellular unipolar electrode during electrical stimulation. The recordings were obtained from the bullfrog atrium along the transverse and longitudinal fiber directions. These recordings revealed the presence of a uniformly polarized region in the transverse fiber direction. In the longitudinal fiber direction, adjacent oppositely polarized regions coexist. These results are qualitatively predicted by an activation function developed for uncoupled fibers. They contradict the predictions of conventional cable models and also support newly developed models of cardiac tissue.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Widely tunable femtosecond fiber laser (2008)

The possibility to build up an optical source of femtosecond pulses that are smoothly tuned in the telecommunication range using a dispersion-decreasing fiber is demonstrated. The smooth tuning is based on the Raman frequency conversion of ultrashort pulses, which can be effectively tuned due to the compression mechanism for maintaining of a relatively high pulse intensity in the medium with a monotonically decreasing anomalous dispersion. The generation of a 90-fs soliton pulse whose wavelength is smoothly tuned in the wavelength range 1.55 - 1.85 &mu;m is experimentally demonstrated.
Virus isolate from carp: genetic characterization reveals a novel picornavirus with two aphthovirus 2A-like sequences (2013)

Picornaviruses have been isolated from a variety of hosts, mainly mammals and birds. Here, we describe the sequence analysis of carp picornavirus 1 (CPV-1) F37/06 that was isolated from an organ pool (heart, brain, liver) of a common carp ( Cyprinus carpio ). This carp perished after an accidental discharge of liquid manure into a fish pond and presented without obvious clinical symptoms. Experimental intraperitoneal infection of young carp with CPV-1 revealed no clinical signs, but the virus was re-isolated from various organs. Sequence analysis of almost the complete genome (7632 nt excluding the poly-A tract) revealed a novel picornavirus clade. In phylogenetic trees, the polymerase sequence clusters with parechoviruses, duck hepatitis A virus, eel picornavirus and aquamavirus A. The ORF includes 6807 nt and encodes a polyprotein of 2269 amino acids. CPV-1 has a genome layout like that of picornaviruses except for the presence of two aphthovirus 2A-like NPGP sequence motifs: VPg+5′UTR[1AB-1C-1D-2A1 npgp /2A2 npgp -2B-2C ATPase /3A-3B VPg -3C pro -3D pol ]3′UTR-poly-A. 2A1 npgp and 2A2 npgp are separated by 133 amino acids. The proteins 2A2 npgp , 2B, 3A and 3B VPg have no significant similarity to the corresponding proteins of other picornaviruses. Amino acid identities of the orthologous proteins P1, 2C, 3C pro and 3D pol range from 16.4 to 40.8 % in the eel picornavirus/CPV-1 comparison. 3D pol shows the closest similarity to eel picornavirus, with an amino acid identity of 40.8 %, followed by human parechovirus (36.5 %), duck hepatitis A virus (32.7 %) and swine pasivirus (29.3 %). Both the unique genome organization and low sequence similarity support the assignment of CPV-1 to a novel picornavirus species within a novel genus.
Development of laser-based techniques for rapid prototyping of printed wiring boards (2006)

For rapid prototyping and repair of printed wiring boards, direct laser patterning techniques of Cu are investigated. Laser direct writing techniques reported in the literature are compared and contrasted. The approach taken in this work is laser-induced Ag seeding of the substrate followed by subsequent electroless Cu plating. The Ag seeds were deposited on FR4 and Al2O3 substrates using 3rd harmonic Nd:YVO4 and CO2 laser beams. Laser-written Ag seeds were catalytic for electroless deposition of Cu. A minimum resistivity of 6 µΩ-cm was obtained.
Intelligent Robotic Vision Systems (2020)

This chapter covers the current weaknesses and the directions of current research. It focuses on all relevant aspects, which means that vision computers are treated as part of an environment that includes feeding systems, robots, light sources, and sensors. The chapter describes both software and hardware for image processing. It deepens the interactions of the vision system with the robot and the part transport systems. The chapter provides an overview of the different aspects of a vision system. It also describes the different steps of choosing a good illumination technique, selecting the appropriate type of sensor, and then defining the spatial sampling and gray-level quantization characteristics and selecting the vision computer. The chapter concentrates on algorithms for the three major tasks of robot vision: object recognition and localization, visual servoing, and visual inspection. The reader should be familiar with basic image-processing techniques, such as convolution, Fourier transforms, thresholding, and run length encoding.
An inexpensive technique for handling solvent-dependent crystals (1994)

A new method of handling solvent-dependent crystals is described. This involves using a bubble of hexadecane to reduce solvent diffusion. The capillary tube is scaled with araldite resin.
Photoallylation of Quinones with Allylstannane (1986)

Abstract Photochemical reactions of halogeno-quinones with allylstannane in a benzene or an acetonitrile solution afforded allylated quinones and allyl quinols as major products. Nuclear spin polarization effects observed during irradiation support a contribution of electron transfer process.
Floating point CORDIC-based architecture for powering computation (2015)

This work presents an architecture for powering computation in floating point arithmetic that is based on an expanded hyperbolic CORDIC algorithm, where the user can select the 2-D domain of convergence that suits their application. The fully parameterized hardware implementation allows us to explore trade-offs among design parameters (numerical format, number of iterations), resource usage, accuracy, and execution time. We carry out an exhaustive design space exploration and generate Pareto-optimal realizations in the resource-accuracy space. Our approach allows us to select optimal hardware realizations that meet or exceed accuracy requirements.
Mechanisms of Hepatitis C Virus Escape from Vaccine-Relevant Neutralizing Antibodies (2021)

Hepatitis C virus (HCV) is a major causative agent of acute and chronic hepatitis. It is estimated that 400,000 people die every year from chronic HCV infection, mostly from severe liver-related diseases such as cirrhosis and liver cancer. Although HCV was discovered more than 30 years ago, an efficient prophylactic vaccine is still missing. The HCV glycoprotein complex, E1/E2, is the principal target of neutralizing antibodies (NAbs) and, thus, is an attractive antigen for B-cell vaccine design. However, the high genetic variability of the virus necessitates the identification of conserved epitopes. Moreover, the high intrinsic mutational capacity of HCV allows the virus to continually escape broadly NAbs (bNAbs), which is likely to cause issues with vaccine-resistant variants. Several studies have assessed the barrier-to-resistance of vaccine-relevant bNAbs in vivo and in vitro. Interestingly, recent studies have suggested that escape substitutions can confer antibody resistance not only by direct modification of the epitope but indirectly through allosteric effects, which can be grouped based on the breadth of these effects on antibody susceptibility. In this review, we summarize the current understanding of HCV-specific NAbs, with a special focus on vaccine-relevant bNAbs and their targets. We highlight antibody escape studies pointing out the different methodologies and the escape mutations identified thus far. Finally, we analyze the antibody escape mechanisms of envelope protein escape substitutions and polymorphisms according to the most recent evidence in the HCV field. The accumulated knowledge in identifying bNAb epitopes as well as assessing barriers to resistance and elucidating relevant escape mechanisms may prove critical in the successful development of an HCV B-cell vaccine.