gebhart/patrepeval huggingface dataset:
Mechanisms of Receptor-mediated Transmembrane Communication (1986)

Our experiments with the hIR protein have been designed to address a very general question of transmembrane receptor structure and function: What are the roles and interactions of the various deduced structural domains of such molecules in the initiation of the response of cells to extracellular signals? All of the evidence to date supports the previous hypothesis based on biochemical data that the IR requires ligand-activated TPK functions to initiate the insulin response by cells (for review, see Kahn 1985). Thus, mutations that compromise hIR TPK activity (site-directed point mutations or deletions) result in a concomitant decrease in at least one aspect of insulin action (glucose uptake; Ellis et al. 1986a). Other studies utilizing microinjection of antibodies to inhibit the receptor kinase have extended this conclusion to include a critical role for the receptor kinase in insulin's ability to stimulate ribosomal protein S6 phosphorylation in CHO cells, glycogen synthetase in hepatoma cells, glucose uptake in adipocytes (Morgan and Roth 1987), and frog oocyte maturation (Morgan et al. 1986). Second, analyses of cell lines that express experimentally truncated hIR TPKs demonstrate that, when membrane-anchored, this TPK domain is in fact capable of autonomous hormone-independent IR function: Such cells exhibit a constitutively elevated, insulin-independent uptake of 2-deoxyglucose (Ellis et al. 1987). Finally, by substitution of a homologous TPK for that of hIR, we find that although such a hybrid is capable of insulin-dependent transmembrane signaling (phosphorylation of the hybrid beta-subunit on tyrosine residues), the hybrid IR.ros molecule does not function as an IR in such cells: It mediates neither short-term (uptake of 2-deoxyglucose) nor long-term (incorporation of [3H]thymidine) effects of insulin (L. Ellis et al., in prep.). Together, these results suggest that (1) the hIR TPK domain conveys a substrate specificity for the insulin response and (2) that a functional hIR extracellular domain alone is not sufficient for generation of the insulin response (e.g., ligand-induced aggregation, or simple delivery of insulin into the cell). With the linking of the extracellular and cytoplasmic domains of the hIR molecule has evolved a cellular mechanism for the control of hIR TPK activity; the result is that cells which express the IR are now insulin responsive, and the physiological responses associated with the hormone are ligand-activated. Thus, the uncontrolled state of autonomous TPK activity, with the associated constitutive physiological response (e.g., as exhibited by the spBam hIR mutant), is circumvented.(ABSTRACT TRUNCATED AT 400 WORDS)
Amanitin Greatly Reduces the Rate of Transcription by RNA Polymerase II Ternary Complexes but Fails to Inhibit Some Transcript Cleavage Modes (1996)

The toxin alpha-amanitin is frequently employed to completely block RNA synthesis by RNA polymerase II. However, we find that polymerase II ternary transcription complexes stalled by the absence of NTPs resume RNA synthesis when NTPs and amanitin are added. Chain elongation with amanitin can continue for hours at approximately 1% of the normal rate. Amanitin also greatly slows pyrophosphorolysis by elongation-competent complexes. Complexes which are arrested (that is, which have paused in transcription for long periods in the presence of excess NTPs) are essentially incapable of resuming transcription in the presence of alpha-amanitin. Complexes traversing sequences that can provoke arrest are much more likely to stop transcription in the presence of the toxin. The substitution of IMP for GMP at the 3' end of the nascent RNA greatly increases the sensitivity of stalled transcription complexes to amanitin. Neither arrested nor stalled complexes display detectable SII-mediated transcript cleavage following amanitin treatment. However, arrested complexes possess a low level, intrinsic transcript cleavage activity which is completely amanitin-resistant; furthermore, pyrophosphorolytic transcript cleavage in arrested complexes is not affected by amanitin.
PreﬁxSpan: Mining Sequential Patterns by Preﬁx-Projected Pattern (2011)

Sequential pattern mining discovers frequent subsequences as patterns in a sequence database. Most of the previously developed sequential pattern mining methods, such as GSP, explore a candidate generation-and-test approach However, this approach may not be efficient in mining large sequence databases having numerous patterns and/or long patterns. In this paper, we propose a projection-based, sequential pattern-growth approach for efficient mining of sequential patterns. In this approach, a sequence database is recursively projected into a set of smaller projected databases, and sequential patterns are grown in each projected database by exploring only locally frequent fragments. Based on an initial study of the pattern growth-based sequential pattern mining, FreeSpan, we propose a more efficient method, called PSP, which offers ordered growth and reduced projected databases technique is developed in PrefixSpan.
Ultrashallow Junction Formation by Self-Limiting LTP and Its Application to Sub-65-nm Node MOSFETs (2005)

We have developed a novel laser thermal process that dramatically enhances laser exposure windows by controlling the heating process in a self-limiting way. Key technology is realized by introducing a new process combination of preamorphization implantation and a heat absorber with a phase switch layer, and by optimizing them. The V/sub th/ rolloffs of MOSFETs formed by this method were remarkably improved compared to those by rapid thermal annealing when offset-spacer and halo-implantation processes were not applied. Its effectiveness was also verified in 50-nm gate complementary metal-oxide semiconductor devices for the first time by confirming that the drain current increased with laser fluence beyond the conventional exposure limit.
<title>MPEG-7 audio-visual indexing test-bed for video retrieval</title> (2003)

This paper reports on the development status of a Multimedia Asset Management (MAM) test-bed for content-based indexing and retrieval of audio-visual documents within the MPEG-7 standard. The project, called "MPEG-7 Audio-Visual Document Indexing System" (MADIS), specifically targets the indexing and retrieval of video shots and key frames from documentary film archives, based on audio-visual content like face recognition, motion activity, speech recognition and semantic clustering. The MPEG-7/XML encoding of the film database is done off-line. The description decomposition is based on a temporal decomposition into visual segments (shots), key frames and audio/speech sub-segments. The visible outcome will be a web site that allows video retrieval using a proprietary XQuery-based search engine and accessible to members at the Canadian National Film Board (NFB) Cineroute site. For example, end-user will be able to ask to point on movie shots in the database that have been produced in a specific year, that contain the face of a specific actor who tells a specific word and in which there is no motion activity. Video streaming is performed over the high bandwidth CA*net network deployed by CANARIE, a public Canadian Internet development organization.
Bandgap-Engineered in Indium–Gallium–Oxide Ultraviolet Phototransistors (2015)

The electrical performance of amorphous indium gallium oxide (a-IGO) thin-film transistors applied as deep-ultraviolet (DUV) phototransistors is investigated. It was found that the bandgap of a-IGO can be engineered by altering its chemical composition. The performance of the phototransistors depended strongly on In <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> content in the IGO film. When the indium content increases from 21% to 31%, the phototransistor cutoff red-shifted from 280 to 320 nm. The DUV-to-visible rejection ratio and photoresponsivity of the fabricated phototransistors were ~10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sup> and 0.18 A/W.
Sequential Delivery of Dual Growth Factors from Injectable Chitosan-Based Composite Hydrogels (2019)

Local administration of platelet-derived growth factor-BB (PGDF-BB) and bone morphogenetic protein-2 (BMP-2) in a sequential release manner could substantially promote bone healing. To achieve this goal, a delivery system that could sustain the release of PGDF-BB and BMP-2 by way of temporal separation was developed. One type of PGDF-BB-encapsulated alginate microsphere and another type of BMP-2-encapsulated microsphere with a core-shell structure were respectively produced using emulsification methods. These two types of microspheres were then embedded into chitosan/glycerophosphate hydrogel for constructing composite gels. Some of them were found to be injectable at ambient temperature and had thermo-sensitive features near physiological temperature and pH. The optimally formulated composite gels showed the ability to control the release of PGDF-BB and BMP-2 in a sequential fashion in which PDGF-BB was released earlier than BMP-2. In vitro release patterns indicated that the release rates could be significantly regulated by varying the embedded amount of the factor-encapsulated microspheres, which can in turn mediate the temporal separation release interval between PGDF-BB and BMP-2. The released PDGF-BB and BMP-2 were detected to be bioactive based on their respective effects on Balb/c 3T3 and C2C12 cells. These results suggest that the presently developed composite gels have the potential for bone repair by synergistically utilizing the early chemotactic effect of PDGF-BB and the subsequent osteogenic and angiogenic functions of PDGF-BB and BMP-2.
Face Recognition using Energy Probability in DCT Domain (2006)

In this paper, we propose a novel feature extraction method for face recognition. This method is based on discrete cosine transform (DCT), energy probability (EP), and linear discriminant analysis (LDA). We define an energy probability as magnitude of effective information. It is used to create a frequency mask in DCT domain. Our method consists of three steps. First, the spatial domain of face images is transformed into the frequency domain called DCT domain. Second, for energy probability is applied on DCT domain which acquires from face image, dimension reduction of data and optimization of valid information. At last, in order to obtain the most significant feature of face images, LDA is applied to the extracted data using frequency mask. Our experimental results show that the proposed method improves on the dimension reduction of feature space and the face recognition over the previously proposed methods
Abstract 790: Mutations and copy number gains of EGFR and KRAS genes in lung adenocarcinomas (2010)

Abstract Background: Activating mutations or copy number gains (CNGs) of epidermal growth factor receptor (EGFR) and KRAS genes are major oncogenic alterations in lung adenocarcinomas. While mutations of these genes are well-known to be mutually exclusive each other, the interrelationship of four genetic alterations (mutations or CNGs of EGFR or KRAS gene) has never been investigated. Material and methods: We determined these four genetic alterations in 314 lung adenocarcinoma cases including 176 Asians and 138 Non-Asians from four countries with complete clinical information. Mutational and CNG statuses of EGFR or KRAS gene were determined by direct sequencing and quantitative PCR method, respectively. Results: EGFR mutations (mEGFR), EGFR CNGs (gEGFR), KRAS mutations (mKRAS) and KRAS CNGs (gKRAS) were present in 25%, 35%, 23% and 6% of 314 cases, respectively (Table 1). Either alteration of EGFR or KRAS gene was present in 41% and 20% of all cases, respectively. Alterations of either EGFR or KRAS gene were observed in 67% of 314 cases, that was significantly more frequent in Asian never smokers (84%) than other groups (61%, P = 0.0001). Of note, CNGs of both genes preferentially occurred in their respective mutant case (P = 0.001 in EGFR and P = 0.04 in KRAS). mEGFR and mKRAS were completely mutually exclusive each other while gEGFR and gKRAS were not. Either alteration of EGFR was also exclusively present with either alteration of KRAS (P &amp;lt; 0.0001) while 19 cases (6%) had alterations of both genes. Interestingly, gKRAS was detected in one case among 79 mEGFR cases (1.3%) while gEGFR could be observed in 10 cases among 71 mKRAS cases (14.1%, P = 0.003). Conclusion: Activations of EGFR and/or KRAS genes are present in about 70% of lung adenocarcinomas. Activations of EGFR or KRAS gene are almost exclusive and activating mutation of EGFR rarely accompanies KRAS activation, suggesting that two genetic alterations independently associate with oncogenesis of lung adenocarcinomas.Table 1.Activated pathway of 314 lung adenocarcinomasActivated pathwayTypes of activationn%Both genesSubtotal196.1 m, gEGFR + gKRAS10.3 gEGFR + m, gKRAS51.6 gEGFR + mKRAS51.6 gEGFR + gKRAS82.5 EGFR gene aloneSubtotal12941.1 mEGFR3912.4 m, gEGFR3912.4 gEGFR5116.2 KRAS gene aloneSubtotal6219.7 mKRAS5818.5 m, gKRAS31.0 gKRAS10.3 NoneSubtotal10433.1 Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 790.
Lymphocytic Choriomeningitis Virus Clone 13 Infection Results in CD8 T Cell–Mediated Host Mortality in Diacylglycerol Kinase α–Deficient Mice (2023)

Diacylglycerol is a potent element of intracellular secondary signaling cascades whose production is enhanced by cell-surface receptor agonism and function is regulated by enzymatic degradation by diacylglycerol kinases (DGKs). In T cells, stringent regulation of the activity of this second messenger maintains an appropriate balance between effector function and anergy. In this article, we demonstrate that DGKα is an indispensable regulator of TCR-mediated activation of CD8 T cells in lymphocytic choriomeningitis virus Clone 13 viral infection. In the absence of DGKα, Clone 13 infection in a murine model results in a pathologic, proinflammatory state and a multicellular immunopathologic host death that is predominantly driven by CD8 effector T cells.
Glucocorticoid-Treated Mice Have Localized Changes in Trabecular Bone Material Properties and Osteocyte Lacunar Size That Are Not Observed in Placebo-Treated or Estrogen-Deficient Mice (2006)

Based on these results, it seems that GCs may have direct effects on osteocytes, resulting in a modification of their microenvironment. These changes, including an enlargement of their lacunar space and the generation of a surrounding sphere of hypomineralized bone, seem to produce highly localized changes in bone material properties that may influence fracture risk.
Adesão e resistência a práticas de agricultura biológica entre agricultores familiares: reflexões a partir de uma abordagem com grupos focais (2018)

The rapid growth and the public and political attention to biological agriculture promote relevant reflections from a sociological point of view. The market of biological production, whose principles are based on concerns about economical, environmental and social sustainability, gains legitimacy mainly through consumers who give preference, when shopping for food, to healthy and environmentally friendly conditions and means of production. In addition, home farming, through its own characteristics, takes on board the basic principles of biological agriculture. This article attempts to discuss the first results of an Action Research project about the Bridges between Biological Agriculture and Home Farming. The aim is to, through an exploratory qualitative research, based on documental analysis and the work of 2 focus groups: (i) isolate both the facilitating and obstructing factors of practicing biological agriculture; (ii) understand existing processes of resistance to change by home farmers. In order to recommend future public policies, be it at local, regional or national levels, there must be an understanding of both constraints and facilitators of practicing sustainable agriculture, which point at very distinct representations. Amongst the favourable representations it is possible to highlight the health and environmental benefits, some proximity between traditional agricultural practices by home farmers and the principles and techniques of biological agriculture, technical support and some political will both at local and national levels. However, the small amount of years farmers spent at school and the lack of technical knowledge makes this practice of agriculture focused in shortterm profitability, adding to the difficulties of a small and distant market, the high cost of the products, and the lack of effective product flowing channels.
Photoluminescence, electroluminescence, and scintillation of halide perovskites (Conference Presentation) (2020)

We present our recent research results on luminescence of halide perovskites under various excitation sources: photoexcitation, electrical current and high-energy radiation. Our photonics crystal can reduce the emission rate of perovskite film by preventing the photoluminescence coupling to the film, but enhance the out-coupling efficiency by 23.5 times. Our solution-grown single-crystal perovskite hetero-structure was successful with different halide compositions. A pristine single-crystal light emitting device was demonstrated with excellent protection and encapsulation from material synthesis to device characterization. Lastly, with engineered perovskite materials we demonstrate a multifunctional scintillator for high-energy radiation from X-rays, gamma rays to thermal neutrons.
Unraveling the Molecular Mechanisms Underlying Complement Dysregulation by Nephritic Factors in C3G and IC-MPGN (2018)

Membranoproliferative glomerulonephritis (MPGN) was recently classified as C3 glomerulopathies (C3G), and immune-complex (IC) mediated MPGN. Dysregulation of the complement alternative pathway, driven by acquired and/or genetic defects, plays a pathogenetic role in C3G. However, alternative pathway abnormalities were also found in IC-MPGN. The most common acquired drivers are the C3 nephritic factors (C3NeFs), heterogeneous autoantibodies that stabilize the C3 convertase, C3bBb. C3NeFs are traditionally detected by hemolytic assays based on sheep erythrocyte lysis, which however do not provide a direct molecular estimation of C3bBb formation and decay. We set up a microplate/western blot assay that specifically detects and quantifies C3bBb, and its precursor, the C3 proconvertase C3bB, to investigate the complex mechanistic effects of C3NeFs from patients with primary IC-MPGN (<i>n</i> = 13) and C3G (<i>n</i> = 13). In the absence of properdin, 9/26 patients had C3NeF IgGs stabilizing C3bBb against spontaneous and FH-accelerated decay. In the presence of properdin the IgGs of all but one patient had C3bBb-stabilizing activity. Properdin-independent C3NeFs were identified mostly in DDD patients, while properdin-dependent C3NeFs associated with either C3GN or IC-MPGN and with higher incidence of nephrotic syndrome. When we grouped patients based on our recent cluster analysis, patients in cluster 3, with highly electron-dense intramembranous deposits, low C3, and mostly normal sC5b-9 levels, had a higher prevalence of properdin-independent C3NeFs than patients in clusters 1 and 2. Conversely, about 70% of cluster 1 and 2 patients, with subendothelial, subepithelial, and mesangial deposits, low C3 levels and high sC5b-9 levels, had properdin-dependent C3NeFs. The flexibility of the assay allowed us to get deep insights into C3NeF mechanisms of action, showing that: (1) most C3NeFs bind strongly and irreversibly to C3 convertase; (2) C3NeFs and FH recognize different epitopes in C3 convertase; (3) C3NeFs bind rapidly to C3 convertase and antagonize the decay accelerating activity of FH on newly formed complexes; (4) C3NeFs do not affect formation and stability of the C3 proconvertase. Thus, our study provides a molecular approach to detecting and characterizing C3NeFs. The results highlight different mechanisms of complement dysregulation resulting in different complement profiles and patterns of glomerular injury, and this may have therapeutic implications.
Wat zijn de belangrijkste problemen in de gemeente (2016)

In dit hoofdstuk laten we zien:<br/>• Problemen op het gebied van ‘Verkeer en fysieke leefomgeving’ (21,8%) en ‘Politiek en bestuur’ (19,6%) worden het vaakst genoemd als belangrijkste probleem in de gemeente. Gevolgd door ‘Criminaliteit en veiligheid’ (10,7%) en ‘Immigratie en integratie’ (10,6%).<br/>• De lokale politiek wordt regelmatig, met name in kleinere gemeenten en door mensen ouder dan 65, beschuldigd van laakbaar gedrag.<br/>• ‘Criminaliteit en veiligheid’ wordt voornamelijk in de grote steden met veel niet-westerse allochtonen als probleem aangekaart, zeker door middelbaar- en hoogopgeleiden en jongeren.<br/>• ‘Immigratie en integratie’ focust vooral op de vluchtelingen en AZCs, en dit wordt vaker door jongeren als probleem gezien.
Bootstrapping the NPOI: keeping long baselines in phase by tracking fringes on short baselines (1998)

The high-spatial-frequency fringes that contain detailed information about a stellar image are generally too weak to track. The Navy Prototype Optical Interferometer (NPOI) is the first interferometer to measure these fringes with the techniques of phase bootstrapping - the use of fringe tracking on two short baselines AB and BC to keep a longer baseline AC phased up - and wavelength bootstrapping - the use of fringe tracking at long wavelengths to keep the long baseline phased up at short wavelengths. We demonstrate the utility of phase and wavelength bootstrapping with the NPOI observations of stellar limb darkening of (beta) Cancri of Pauls et al. in these proceedings. The NPOI baselines and wavelength coverage were 19, 22, and 38 m and (lambda) 450 to (lambda) 850 nm. For (lambda) &lt; 675 nm, the 37 m baseline samples the uv plane beyond the first null of the Airy disk.
Miniature ceramic filter-antenna for wireless communications systems at 60GHz (2014)

This study presents a new design of a multi-band filter-antenna subsystem dedicated to high-speed wireless communications at 60 GHz. The objective is to design the subsystem as a monolithic object realized with a ceramic material. The broadband antenna is composed of a conical horn acting as a primary source for illuminating a reflect-array. The 9-GHz spectrum, which covers the unlicensed 60-GHz band, is divided into four channels of 1.65 GHz. In this paper, the primary source and the four bandpass filters are designed and integrated together.
Rate-of-Change Dependence of the Performance of Two CGM Systems During Induced Glucose Swings (2015)

Both systems were affected by rapidly changing BG concentrations to some degree, although system B was mostly unaffected by decreasing BG concentrations. It would seem that technological advancements in CGM systems might allow for a more precise tracking of BG concentrations even at rapidly changing BG concentrations.
An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus (2013)

We show that LDVICp016 is a secreted protein belonging to the TNF receptor family that may be part of a larger gene family in Lymphocystiviruses. While the ligand of this protein remains unknown, possibly due to the species specific nature of this interaction, further investigations into the potential role of this protein in the blockade of immune responses in its fish host are required.