gebhart/patrepeval huggingface dataset:
Equilibrium distances for the capillary interaction between floating objects (2021)

When small objects are placed at a water-air interface, attractive and repulsive interactions appear due to liquid deformations. Although it is commonly admitted that two floating objects deforming the liquid interface in the same way are only attracting, we show that in the case of objects whose height does not vary during the interaction, the situation is much more complex than expected. In fact, attraction and repulsion can coexist at different ranges, so that equilibrium distances are observed. A 1D model based on the capillary interaction between vertical plates immersed in water is used to illustrate and calculate these situations, giving a picture of capillary interactions. We show that the wetting condition plays a determinant role in the behaviour of the interaction between floating objects. We also demonstrate that the equilibrium distance is given by the logarithm of the capillary charge ratio, using the right capillary charge definition. We also discuss the particular case of the existence of an interaction with a zero-capillary charge. A general equation of the equilibrium distance is proposed. An experimental confirmation of this relation is also given.
Atlas of Cell Organelles Fluorescence (2003)

Containing over 150 original photomicrographs accompanied by protocol information, Atlas of Cell Organelles Fluorescence delineates organelles structures, interaction, and organization into complexes. It provides a collection that shows living cells under physiopathological conditions and in the context of treatment with carcinogens, xenobi
Trial in progress: A phase I study of AMG 199, a half-life extended bispecific T-cell engager (HLE BiTE) immune therapy, targeting MUC17 in patients with gastric and gastroesophageal junction (G/GEJ) cancer. (2020)

TPS4649 Background: Prognosis for advanced G/GEJ cancer is poor and new treatment modalities are urgently needed. MUC17 is a transmembrane protein overexpressed and differentially localized on the cell membrane of G/GEJ cancer cells; expression and localization in normal cells is much more limited. AMG 199 is an HLE BiTE immune therapy designed to engage CD3-positive T cells to MUC17-positive G/GEJ cancer cells, mediate redirected tumor cell lysis, and induce T cell activation and proliferation. A clinical trial is being conducted for this novel and targeted immune therapy agent in patients with MUC17-positive G/GEJ cancer. Methods: This is a first-in-human phase 1, open-label, dose escalating study (NCT04117958) evaluating AMG 199 in patients with MUC17-positive G/GEJ cancer. Key eligibility criteria include metastatic or locally advanced unresectable MUC17-positive (as determined by IHC using a central laboratory assay) gastric adenocarcinoma or gastroesophageal junction adenocarcinoma ineligible for curative surgery and relapsed or treatment-refractory following ≥2 lines including a platinum, a fluoropyrimidine, taxane or irinotecan, and an approved vascular endothelial growth factor receptor antibody or tyrosine kinase inhibitor. Patients eligible for human epidermal growth factor receptor 2 (HER2) directed therapy should have received an approved HER2 targeting antibody. Primary endpoints include: dose-limiting toxicities, treatment-emergent or -related adverse events, vital signs, electrocardiogram (ECG), and laboratory changes. Secondary endpoints include: pharmacokinetics of AMG 199, objective response, duration of response, time to progression, 6-month and 1-year progression-free survival, and 1-year and 2-year overall survival. The dose exploration (n = 30) will estimate the maximum tolerated dose and/or recommended phase 2 dose; this will be followed by a dose expansion (n = 40) and evaluation of the benefit/risk profile of AMG 199. The study began enrolling patients in January 2020 and is ongoing. This is the first clinical trial to investigate MUC17 as a potential anti-tumor target. For more information, please contact Amgen Medical Information: medinfo@amgen.com . Clinical trial information: NCT04117958 .
The Multiple Carrier Model of Nonribosomal Peptide Biosynthesis at Modular Multienzymatic Templates (1996)

Gramicidin S synthetase 1 and 2 were affinity-labeled at their thiolation centers either by thioesterification with the amino acid substrate or by specific alkylation with the thiol reagent N-ethylmaleimide in combination with a substrate protection technique. The labeled proteins were digested either chemically by cyanogen bromide or by proteases. An efficient multistep high pressure liquid chromatography methodology was developed and used to isolate the active site peptide fragments of all five thiolation centers of gramicidin S synthetase in pure form. The structures of these fragments are investigated by N-terminal sequencing, mass spectrometry, and amino acid analysis. Each of the active site peptide fragments contains the consensus motif LGG(H/D)S(L/I), which is specific for thioester formation in nonribosomal peptide biosynthesis. It was demonstrated that a 4'-phosphopantetheine cofactor is attached to the central serine of the thiolation motif in each amino acid-activating module of the gramicidin S synthetase multienzyme system forming the thioester binding sites for the amino acid substrates and catalyzing the elongation process. Our data are strong support for a "multiple carrier model" of nonribosomal peptide biosynthesis at multifunctional templates, which is discussed in detail.
Characteristics of a rf-only hexapole ion-guide interface for plasma-source time-of-flight mass spectrometry (2001)

A hexapole collision cell has been designed and incorporated into an orthogonal extraction plasma-source time-of-flight mass spectrometer (TOFMS). The ion-guide assembly was constructed from 20 cm long, 3.175 mm diameter stainless steel rods. The cell is positioned between the second and third vacuum stages of the mass spectrometer. The device was characterized with an inductively coupled plasma ionization source (ICP). Pressurization of the cell with a buffer gas (such as helium or hydrogen) lowers the axial energy of the ions, and produces a beam having a most probable energy of 1.3 eV and an energy spread of less than 2 eV. The signal levels were found to be strongly influenced by pressure in the cell, rf voltage applied to the rods, and operating frequency of the ion guide. Lighter ions (m/z 7) were transmitted more efficiently at higher frequencies and reduced rf voltages, while signals for heavier ions (m/z 238) were greater at lower frequencies and elevated voltages. Detection limits with the conventional static ion optics and hexapole collision cell were comparable, in the range of 0.4–2 ng ml−1 and similar when either helium or hydrogen collision gas was used. Precision for the peak height measurement was 5.4% RSD with the hexapole ion guide, slightly better than when static optics were employed (7.3% RSD). The strong signals from 40Ar+ could be reduced by four orders of magnitude through use of hydrogen as a collision gas because of efficient and selective charge-exchange reactions. Isotope ratio precision was found to be counting-statistics limited, with the best precision obtained for the isotopes of silver at 0.043% RSD (n = 7) for 10 million sequentially summed spectra. The mass resolving power (FWHM) of the spectrometer was improved through the use of the hexapole ion-guide assembly (2915) relative to that obtained with static ion optics (1510).
Phenothiazine drugs: structure-activity relationships explained by a conformation that mimics dopamine. (1975)

The antischizophrenic activity of phenothiazine drugs and their tendency to elicit extrapyramidal symptoms are thought to involve blockade of synaptic dopamine receptors in the brain. Space filling molecular models show how favorable Van der Waal's interactions between the side chain amino of phenothiazines and the 2-substituent on ring A can promote a conformation mimicking dopamine. These Van der Waal's attractive forces can expain (i) the greater potency of drugs with trifluoromethyl rather than chlorine as a 2-substituent; (ii) the enhanced activity of phenothiazines with piperazine instead of alkylamino side chains; (iii) the increased potency associated with hydroxyethylpiperazines as contrasted to piperazine side chains; (iv) the greater potency of cis rather than trans thioxanthenes; and (v) the crucial location of the ring A substituent at carbon no. 2. Potential energy calculations support the observations with molecular models and suggest an active conformation for the phenothiazines.
Is There an Immortal Memory? (2004)

Today's technologies for storing data have vexing limitations: Dynamic random access memory chips only retain data for short times, hard disk drives are slow relative to processor speed, and flash memory (like that in digital cameras) is expensive. Researchers are thus searching for a fast, inexpensive memory technology that retains data even when the power is off. As [Scott][1] discusses in his Perspective, the search has identified several interesting avenues that are being actively pursued. But the real-world challenges are severe, and proponents of nonvolatile memory need to take a hard look at the practical realities before declaring success.

 [1]: http://www.sciencemag.org/cgi/content/full/304/5667/62
Inorganic/Organic Doped Carbon Aerogels As Biosensing Materials for the Detection of Hydrogen Peroxide (2013)

In this article, three different inorganic/organic doped carbon aerogel (CA) materials (Ni-CA, Pd-CA, and Ppy-CA) were, respectively, mixed with ionic liquid (IL) to form three stable composite films, which were used as enhanced elements for an integrated sensing platform to increase the surface area and to improve the electronic transmission rate. Subsequently, the effect of the materials performances such as adsorption, specific surface area and conductivity on electrochemistry for myoglobin (Mb) was discussed using N2 adsorption-desorption isotherm measurements, scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). Moreover, they could act as sensors toward the detection of hydrogen peroxide (H2O2) with lower detection limits (1.68 μM, 1.02 μM, and 0.85 μM, for Ni-CA/IL/Mb-CPE, Pd-CA/IL/Mb-CPE, and Ppy-CA/IL/Mb-CPE, respectively) and smaller apparent Michaelis-Menten constants KM. The results indicated that the electroconductibility of the doped CA materials would become dominant, thus playing an important role in facilitating the electron transfer. Meanwhile, the synergetic effect with [BMIm]BF4 IL improved the capability of the composite inorganic/organic doped CA/IL matrix for protein immobilization. This work demonstrates the feasibility and the potential of a series of CA-based hybrid materials as biosensors, and further research and development are required to prepare other functional CAs and make them valuable for more extensive application in biosensing.
Release of vasoactive substances from endothelial cells by shear stress and purinergic mechanosensory transduction (1999)

The evidence for release of vasoactive substances from endothelial cells in response to shear stress caused by the viscous drag of passing fluids is reviewed and, in particular, its physiological significance both in short-term regulation of blood vessel tone and in long-term regulation of cell growth, differentiation, proliferation, and cell death in pathophysiological conditions is discussed. A new concept of purinergic mechanosensory transduction, particularly in relation to nociception, is introduced. It is proposed that distension of tubes (including ureter, vagina, salivary and bile ducts, gut) and sacs (including urinary and gall bladders, and lung) leads to release of ATP from the lining epithelium, which then acts on P2X2/3 receptors on subepithelial sensory nerves to convey information to the CNS.
An InGaAs/InAlAs superlattice avalanche photodiode with a gain bandwidth product of 90 GHz (1991)

The authors describe the fabrication of an InGaAs/InAlAs superlattice avalanche photodiode with a gain-bandwidth product of 90 GHz. The device is composed of an InGaAs/InAlAs superlattice multiplication region and an InGaAs photoabsorption layer. The effect of the superlattice multiplication region thickness on the gain-bandwidth product was studied. A gain-bandwidth product of 90 GHz was obtained for the device with a multiplication region thickness of 0.52 mu m. Low noise performance is compatible with the high gain-bandwidth product due to improvement of the ionization rate ratio made by introducing a superlattice structure into the multiplication region.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Urokinase-plasminogen-activator levels and prognosis in 69 soft-tissue sarcomas (1996)

The local and systemic invasiveness of soft-tissue sarcomas may depend upon an interaction between the primary tumour and the extracellular matrix in which the proteolytic enzyme, urokinase plasminogen activator (uPA), may have an important role. We analyzed the expression of uPA in soft-tissue sarcoma using a luminescent immunoassay technique, and examined the relationships between different uPA levels and tumour characteristics and behaviour. We evaluated 69 adult patients with surgically treated soft-tissue sarcomas (MFH 43, leiomyosarcoma 8, liposarcoma 5, synovial sarcoma 4, others 9) of the extremities and trunk wall. Sixteen developed local recurrences, 26 developed metastases, and 5 had both. The median follow-up for survivors was 55 (30-80) months. The median uPA level was 1.4 (0.04-10.6) ng/mg protein. Increasing uPA levels correlated with increasing grade, malignant fibrous histiocytomas, leiomyosarcomas, DNA non-diploidy, tumour necrosis, local recurrence, and metastasis. Storiform-pleomorphic MFH had higher uPA levels than the myxoid variant. A cut-off value of 0.25 ng/mg protein was identified, above which local recurrence and metastasis occurred more frequently. High uPA levels appear to reflect the malignant phenotype in soft-tissue sarcoma, thus supporting the role of uPA as a prognostic indicator.
Features of Magnetic Phase Transitions in EuB6−xCx (2011)

The ESR measurements were performed on the single crystals EuB6−xCx with x = 0, 0.02 and 0.07 at X band in the temperature range T = 10 − 300 K. It was observed the magnetic phase separation in spin system of Eu2+ ions The reason of the separation is accompanied by the self-localization of free charge carriers and the formation of magnetic polarons of two types. With decreasing temperatures the 2 phase transitions take place. The first transition is connected with the effect of magnetic ordering of the most intensive subsystem. The second transition is obliged to the delocalization of charge carriers and the system is converted from heterogeneous to homogeneous state.
Vibration statistics of thin plates with complex form (1978)

A new mathematical method of calculation of the probabilistic characteristics of mechanical systems with complex geometry is presented. This method has been demonstrated on the example of random vibrating plates. This method is based on the application of certain special fuctions called R functions. In order to demonstrate this method, numerical calculations are presented of probabilistic characteristics for plates with complex geometry which have been clamped on the edge.
Novel Polymorphisms in Promoter Region of ATP Binding Cassette Transporter Gene and Plasma Lipids, Severity, Progression, and Regression of Coronary Atherosclerosis and Response to Therapy (2001)

Identification of mutations in the ATP binding cassette transporter (ABCA1) gene in patients with Tangier disease, who exhibit reduced HDL cholesterol (HDL-C) and apolipoprotein A1 (apoA1) levels and premature coronary atherosclerosis, has led to the hypothesis that common polymorphisms in the ABCA1 gene could determine HDL-C and apoA1 levels and the risk of coronary atherosclerosis in the general population. We sequenced a 660-bp 5' fragment of the ABCA1 gene in 24 subjects and identified 3 novel polymorphisms: -477C/T, -419A/C, and -320G/C. We developed assays, genotyped 372 participants in the prospective Lipoprotein Coronary Atherosclerosis Study (LCAS), and determined the association of the variants with fasting plasma lipids and indices of quantitative coronary angiograms obtained at baseline and 2.5 years after randomization to fluvastatin or placebo. Distribution of -477C/T and -320G/C genotypes were 127 CC, 171 CT, and 74 TT and 130 GG, 168 GC, and 75 CC, respectively, and were in complete linkage disequilibrium (P<0.0001). Data for -477C/T are presented. The -419A/C variant was uncommon (present in 1 of 63 subjects). Heterozygous subjects had a modest reduction in HDL-C (P=0.09) and apoA1 (P=0.05) levels and a lesser response of apoA1 to treatment with fluvastatin (P=0.04). The mean number of coronary lesions causing 30% to 75% diameter stenosis was greater in subjects with the TT genotype (3.1+/-2.1) or CT genotype (2.9+/-1.9) than in subjects with the CC genotype (2.2+/-1.8) (P=0.002). Similarly, compared with subjects with the CC genotype, greater numbers of subjects with the TT or CT genotype had >/=1 coronary lesion (P=0.001). No association between the genotypes and progression of coronary atherosclerosis or clinical events was detected. We conclude that ABCA1 genotypes are potential risk factors for coronary atherosclerosis in the general population.
Masked Hypertension in Diabetes Mellitus: A Potential Risk (2007)

The prevalence and clinical significance of masked hypertension (MHT) in diabetics have infrequently been described. The authors assessed the association of MHT (defined using a clinic blood pressure [BP] <140/90 mm Hg and daytime ambulatory BP > or = 135/85 mm Hg) with microvascular and macrovascular end organ damage in 81 clinically normotensive Japanese diabetic persons. The prevalence of silent cerebral infarcts (SCIs), increased left ventricular mass, and albuminuria were evaluated. Of 81 patients, 38 (46.9%) were classified as having MHT and showed significantly more SCIs (mean +/- SE: 2.5+/-0.5 vs 1.1+/-0.2; P=.017), and more albuminuria (39% vs 16%; P=.025), but no increase in left ventricular mass index, than the normotensive persons in office and on ambulatory BP monitoring group. The prevalence of MHT in this diabetic population was high (47%). Diabetic patients with MHT showed evidence of brain and kidney damage. Hence, out-of-office monitoring of BP may be indicated in diabetics whose BP is normal in the clinic.
Oxidation by metal salts. V. Cobaltic acetate oxidation of alkylbenzenes (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOxidation by metal salts. V. Cobaltic acetate oxidation of alkylbenzenesEl Ahmadi I. Heiba, Ralph M. Dessau, and William J. Koehl Jr.Cite this: J. Am. Chem. Soc. 1969, 91, 24, 6830–6837Publication Date (Print):November 1, 1969Publication History Published online1 May 2002Published inissue 1 November 1969https://pubs.acs.org/doi/10.1021/ja01052a049https://doi.org/10.1021/ja01052a049research-articleACS PublicationsRequest reuse permissionsArticle Views657Altmetric-Citations109LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Alternative Splicing Modulates Autoinhibition and SH3 Accessibility in the Src Kinase Fyn (2009)

Src family kinases are central regulators of a large number of signaling pathways. To adapt to the idiosyncrasies of different cell types, these kinases may need a fine-tuning of their intrinsic molecular control mechanisms. Here, we describe on a molecular level how the Fyn kinase uses alternative splicing to adapt to different cellular environments. Using structural analysis, site-directed mutagenesis, and functional analysis, we show how the inclusion of either exon 7A or 7B affects the autoinhibition of Fyn and how this changes the SH3-dependent interaction and tyrosine phosphorylation of Sam68, with functional consequences for the Sam68-regulated survival of epithelial cells. Our results illustrate a novel mechanism of evolution that may contribute to the complexity of Src kinase regulation.
Brief Report: Adiponectin Levels Linked to Subclinical Myocardial Fibrosis in HIV (2020)

We describe a novel association between serum adiponectin and subclinical intramyocardial fibrosis, as well as a significant inverse relationship between intramyocardial fibrosis and EAT. Adiponectin may represent a target for preventing myocardial injury in the future; however, our findings reflect the complexity of the metabolic interactions of adiponectin and epicardial adipose as factors associated with the myocardial architecture.
A genetic framework for fruit patterning inArabidopsis thaliana (2005)

In the model plant Arabidopsis thaliana, the establishment of organ polarity leads to the expression of FILAMENTOUS FLOWER (FIL) and YABBY3 (YAB3) on one side of an organ. One important question that has remained unanswered is how does this positional information lead to the correct spatial activation of genes controlling tissue identity? We provide the first functional link between polarity establishment and the regulation of tissue identity by showing that FIL and YAB3 control the non-overlapping expression patterns of FRUITFULL (FUL) and SHATTERPROOF (SHP), genes necessary to form stripes of valve margin tissue that allow the fruit to shatter along two defined borders and disperse the seeds. FIL and YAB3 activate FUL and SHP redundantly with JAGGED (JAG), a gene that also promotes growth in organs, indicating that several pathways converge to regulate these genes. These activities are negatively regulated by REPLUMLESS (RPL), which divides FIL/JAG activity, creating two distinct stripes of valve margin.
Sobre la necesidad de una nueva regulación de la guarda y custodia compartida (2011)

El legislador espanol modifico, a traves de la Ley 15/2005, la normativa relativa a la custodia de hijos menores o incapacitados, recogiendo en el art. 92 del Codigo civil la posibilidad de otorgar la custodia compartida a ambos progenitores. Con esta nueva regulacion se pretendia, entre otras cosas, dar respuesta a la nueva realidad social de los ultimos anos en los que el grado de corresponsabilidad de los progenitores con respecto de sus hijos ha aumentado de forma importante, asi como la propia igualdad entre estos.