gebhart/patrepeval huggingface dataset:
Detection of IgD-secreting human lymphocytes at the single cell level by a protein A plaque assay. (1981)

By the use of Staphylococcus aureus protein A-coupled erythrocytes as target cells, and anti-Ig antibodies as a developing serum, immunoglobulin-secreting cells can be detected as plaque-forming cells in a complement-dependent haemolytic plaque assay. This method is based on the fact that the Fc portion of IgG will bind to protein A molecules. Using a rabbit anti-human IgD immunoglobulin as a developing serum we were able to detect secretion of IgD molecules from human cells. In a patient with an IgD myeloma, 6% of the bone marrow cells were found to form plaques. Bone marrow cells and blood lymphocytes from healthy donors as well as human tonsil cells were also examined for delta chain secretion. In some donors, a low increase in plaque numbers was obtained when anti-IgD Ig was used as a developing agent. In spite of the fact that the antisera used were highly specific we cannot rule out the possibility that the increase was due to cross-reactivity. However, these findings clearly demonstrate that the release of IgD from normal human lymphoid cells occurs at a much lower level as compared to secretion of IgM, IgG or IgA.
Ligand-Mediated Assembly and Real-Time Cellular Dynamics of Estrogen Receptor α-Coactivator Complexes in Living Cells (2001)

Studies with live cells demonstrate that agonist and antagonist rapidly (within minutes) modulate the subnuclear dynamics of estrogen receptor alpha (ER) and steroid receptor coactivator 1 (SRC-1). A functional cyan fluorescent protein (CFP)-tagged lac repressor-ER chimera (CFP-LacER) was used in live cells to discretely immobilize ER on stably integrated lac operator arrays to study recruitment of yellow fluorescent protein (YFP)-steroid receptor coactivators (YFP-SRC-1 and YFP-CREB binding protein [CBP]). In the absence of ligand, YFP-SRC-1 is found dispersed throughout the nucleoplasm, with a surprisingly high accumulation on the CFP-LacER arrays. Agonist addition results in the rapid (within minutes) recruitment of nucleoplasmic YFP-SRC-1, while antagonist additions diminish YFP-SRC-1-CFP-LacER associations. Less ligand-independent colocalization is observed with CFP-LacER and YFP-CBP, but agonist-induced recruitment occurs within minutes. The agonist-induced recruitment of coactivators requires helix 12 and critical residues in the ER-SRC-1 interaction surface, but not the F, AF-1, or DNA binding domains. Fluorescence recovery after photobleaching indicates that YFP-SRC-1, YFP-CBP, and CFP-LacER complexes undergo rapid (within seconds) molecular exchange even in the presence of an agonist. Taken together, these data suggest a dynamic view of receptor-coregulator interactions that is now amenable to real-time study in living cells.
Formation of retinoid X receptor homodimers leads to repression of T3 response: hormonal cross talk by ligand-induced squelching. (1993)

Thyroid hormone receptors (TRs) form heterodimers with retinoid X receptors (RXRs). Heterodimerization is required for efficient TR DNA binding to most response elements and transcriptional activation by thyroid hormone. RXRs also function as auxiliary proteins for several other receptors. In addition, RXR alpha can be induced by specific ligands to form homodimers. Here we report that RXR-specific retinoids that induce RXR homodimers are effective repressors of the T3 response. We provide evidence that this repression by RXR-specific ligands occurs by sequestering of RXR from TR-RXR heterodimers into RXR homodimers. This ligand-induced squelching may represent an important mechanism by which RXR-specific retinoids and 9-cis retinoic acid mediate hormonal cross talk among a subfamily of nuclear receptors activated by structurally unrelated ligands.
Tyrosine Biosynthesis in Sorghum bicolor: Isolation and Regulatory Properties of Arogenate Dehydrogenase (1986)

The conversion of prephenic acid to tyrosine can occur by two different routes: (a) oxidative decarboxylation (prephenate dehydrogenase) followed by transamination (aromatic aminotransferase); (b) transamination of prephenate forming the non-aromatic amino acid arogenic acid (prephenate aminotransferase) followed by oxidative decarboxylation (arogenate dehydrogenase). High activity of arogenate dehydrogenase was found in extracts of etiolated sorghum seedlings, while no evidence of prephenate dehydrogenase was observed. Arogenate dehydrogenase from sorghum eluted, with high recovery of activity (93%), as a single peak on DEAE-cellulose chromatography. The enzyme was strongly inhibited by tyrosine but was unaffected by phenylalanine, prephenate, or tryptophan. Kinetic analysis showed that tyrosine inhibition was competitive with arogenate and that the Ki for tyrosine (61 microM) was much smaller than the Km for arogenate (350 microM). The properties of arogenate dehydrogenase indicate that this enzyme is important in the regulation of tyrosine biosynthesis in sorghum. Strong inhibition of the enzyme by tyrosine may indicate that arogenate is a branch point in the shikimate pathway in plants and therefore arogenate may be a precursor to phenylalanine and the numerous phenylpropanoid secondary metabolites derived from phenylalanine.
Retracted: MiR‐449c targets c‐Myc and inhibits NSCLC cell progression (2013)

MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in this study. NSCLC cell proliferation and invasion assays indicate that transfection of miR-449c expression plasmid inhibits the proliferation and invasion ability of NCI-H23 and NCI-H838 cells. In addition, miR-449c overexpression could suppress tumor growth in vivo. Morever, c-Myc was identified as a direct target gene of miR-449c. These findings clearly suggest that miR-449c downregulation and c-Myc amplification may be involved in the development of NSCLC.
Low-power multiband hybrid VCO for 77/81 GHz FMCW long range radar applications (2018)

This paper presents the first multiband hybrid VCO operating between 75 GHz and 81 GHz allocated for the long range FMCW radar applications. The proposed design consists of an accumulation-mode varactor and a capacitor bank which are used for the fine and coarse frequency tuning, respectively. With the multimode tuning technique, the proposed VCO solves the tuning range problem in the coarse tuning path while minimising frequency linearity variation in the fine tuning path. The proposed hybrid VCO is implemented in a TSMC 65 nm CMOS technology. The tuning range of the VCO is from 75 GHz to 80.8 GHz. The simulated average phase noise is -94dBc/Hz at 1 MHz offset between 75 GHz and 80.8 GHz. It dissipates 3.54 mW from 1.2-V supply.
Economic Survival When Markets Are Incomplete (2005)

We consider an infinite horizon economy with incomplete markets with two agents and one good. We begin with an example in which an agent's equilibrium consumption is zero eventually with probability one even if she has correct beliefs and is marginally more patient. We then prove the following general result: if markets are effectively incomplete forever then on any equilibrium path on which some agent's consumption is bounded away from zero eventually, the other agent's consumption is zero eventually. This implies that either some agent vanishes, in that she consumes zero eventually, or the consumption of both agents is arbitrarily close to zero infinitely often. Later we show that the first possibility is a robust outcome since for a wide class of economies with incomplete markets, there are equilibria in which an agent's consumption is zero eventually with probability one even though she has correct beliefs as in the example. Our results mark a sharp contrast with the case studied by Sandroni (2000) and Blume and Easley (2004) where markets are complete.
Wind Power Curve Modeling in Simple and Complex Terrain (2015)

Our previous work on wind power curve modeling using statistical models focused on a location with a moderately complex terrain in the Altamont Pass region in northern California (CA). The work described here is the follow-up to that work, but at a location with a simple terrain in northern Oklahoma (OK). The goal of the present analysis was to determine the gain in predictive ability afforded by adding information beyond the hub-height wind speed, such as wind speeds at other heights, as well as other atmospheric variables, to the power prediction model at this new location and compare the results to those obtained at the CA site in the previous study. While we reach some of the same conclusions at both sites, many results reported for the CA site do not hold at the OK site. In particular, using the entire vertical profile of wind speeds improves the accuracy of wind power prediction relative to using the hub-height wind speed alone at both sites. However, in contrast to the CA site, the rotor equivalent wind speed (REWS) performs almost as well as the entire profile at the OK site. Another difference is that at the CA site, adding wind veer as a predictor significantly improved the power prediction accuracy. The same was true for that site when air density was added to the model separately instead of using the standard air density adjustment. At the OK site, these additional variables result in no significant benefit for the prediction accuracy.
Expression of epoxide hydrolase in insect cells: A focus on the infected cell (1993)

Insect cell culture and the baculovirus vector expression system have emerged to be a promising production technique for heterologous proteins. In this article, expression characteristics for membrane-bound epoxide hydrolase are examined. A generic process is presented whereby cells are grown in serum-free media supplemented with serum and then resuspended in serum-free media to simplify purification after infection. The infected cells retain significant metabolic activity during the postinfection stage. Thus, maintaining nutrient supply during the postinfection period is critical, and a low stirring rate will result in oxygen depletion and shift the metabolism of the infected cells toward lactate production which then lowers product yield. This is the first report indicating that glucose is supplied from sucrose decomposition and then metabolized for viral DNA and recombinant protein production in recombinant baculovirus insect expression system.
Asymmetric Periflexural Exanthem of Childhood: Report of Two New Cases (1994)

A newly described exanthem of infancy consists of an eczematous or scarlatiniform eruption that starts initially from one axillary fold and spreads unilaterally and centrifugally on the trunk and the proximal part of the upper limb. Minor lesions are infrequently present on the contralateral side. The rash may be slightly pruritic and sometimes associated with a moderate regional lymphadenopathy. The appearance in small epidemics and the seasonal prevalence imply a viral agent as the probable origin. We report two new cases of this entity, which appears to be not so rare.
Adeno‐associated virus (AAV) serotypes 2, 4 and 5 display similar transduction profiles and penetrate solid tumor tissue in models of human glioma (2006)

In addition to the widely used AAV2 serotype, AAV4 and AAV5 serotypes may also be used to transduce biologically diverse glioma cell lines. They also penetrate and transduce solid human tumor tissue derived from patient biopsies. Therefore, the data presented here provide a proof of principle for developing AAV4 and AAV5 as treatment vehicles for human malignant gliomas.
New transformation of control points in scaling discrete polygons (1992)

A new mapping transformation method is presented to scale the control points of discrete polygons to locate the transformed coordinate of the control points as accurately as possible. In traditional methods, the area of the control point is regarded as zero and can be suitable only for scaling analog polygons (or a single polygon). By using ourproposed technique, the ratio of black points to totalpoints in resultant polygons can closely approach the original discrete polygons. Our technique eliminates the mapping distortion by taking into account the topology relation among the discrete polygons. We apply our technique to Chinese fonts, English fonts, and mathematical expression symbols, and we compare it to three other transformation techniques.
Synthesis and crystal structure of [26,27-2H6] 24-epi-cathasterone (2002)

The first synthesis of [26,27-2H6]24-epi-cathasterone 8via (20S)-3β-acetoxy-6,6-(ethylenedioxy)-20-formyl-5α-pregnane 5 starting from stigmasterol is described. The aldehyde 5 was alkylated with lithium butyldimethyl-(E)-2,3-dimethyl[3,3,3,4,4,4-2H6]butenylaluminate 6 prepared from 3-[2H3]methyl[4,4,4-2H3]but-1-yne. The structure was determined using spectral data and X-ray crystallographic analysis.
Thermal fluctuation aftereffect of exchange coupled films for spin valve devices (1998)

The annealing-time-dependent change in the unidirectional anisotropy field for exchange coupled films with FeMn, PdPtMn, NiMn, and NiO, which are generally used for spin valve, was investigated after thermal annealing below their blocking temperatures. It was shown that the distribution of local blocking temperature was similar for each exchange coupled film. The annealing-time-dependent change in the unidirectional anisotropy was explained by the use of thermal fluctuation aftereffect model, and the relaxation time for the anisotropy change was found to be much larger for FeMn than for PdPtMn, NiMn, and NiO, when normalizing annealing temperature by their blocking temperatures. From the viewpoint of device application, however, NiMn was proven to be the most promising antiferromagnet in terms of thermal stability because of its highest blocking temperature.
Molecular Sensors and Sensor Arrays Based on Polyaniline Microtubules (1999)

This paper describes the fabrication of microtubular biosensors and sensor arrays based on polyaniline with superior transducing ability. These sensors have been tested for the estimation of glucose, urea, and triglycerides. As compared to that of a macro sensor, the response of the microtubular sensor for glucose is higher by a factor of more than 10(3). Isoporous polycarbonate membranes have been used to fabricate inexpensive devices by simple thermal evaporation of gold using appropriate machined masks. Polyaniline deposition and enzyme immobilization have been done electrochemically. Electrochemical potential control has been used to direct enzyme immobilization to the chosen membrane device and avoid cross talk with adjacent devices. This has enabled the immobilization of a set of three different enzymes on three closely spaced devices, resulting in a microtubule array that can analyze a sample containing a mixture of glucose, urea, and triglycerides in a single measurement. This, in essence, is an "electronic tongue".
Phase-Modulated Decoupling and Error Suppression in Qubit-Oscillator Systems (2015)

We present a scheme designed to suppress the dominant source of infidelity in entangling gates between quantum systems coupled through intermediate bosonic oscillator modes. Such systems are particularly susceptible to residual qubit-oscillator entanglement at the conclusion of a gate period that reduces the fidelity of the target entangling operation. We demonstrate how the exclusive use of discrete shifts in the phase of the field moderating the qubit-oscillator interaction is sufficient to both ensure multiple oscillator modes are decoupled and to suppress the effects of fluctuations in the driving field. This approach is amenable to a wide variety of technical implementations including geometric phase gates in superconducting qubits and the Molmer-Sorensen gate for trapped ions. We present detailed example protocols tailored to trapped-ion experiments and demonstrate that our approach has the potential to enable multiqubit gate implementation with a significant reduction in technical complexity relative to previously demonstrated protocols.
In Utero and Lactational Exposure to Bisphenol A, In Contrast to Ethinyl Estradiol, Does Not Alter Sexually Dimorphic Behavior, Puberty, Fertility, and Anatomy of Female LE Rats (2009)

Many chemicals released into the environment display estrogenic activity including the oral contraceptive ethinyl estradiol (EE2) and the plastic monomer bisphenol A (BPA). EE2 is present in some aquatic systems at concentrations sufficient to alter reproductive function of fishes. Many concerns have been raised about the potential effects of BPA. The National Toxicology Program rated the potential effects of low doses of BPA on behavior and central nervous system (CNS) as an area of "some concern," whereas most effects were rated as of "negligible" or "minimal" concern. However, the number of robust studies in this area was limited. The current study was designed to determine if maternal exposure to relatively low oral doses of EE2 or BPA in utero and during lactation would alter the expression of well-characterized sexually dimorphic behaviors or alter the age of puberty or reproductive function in the female Long-Evans rat offspring. Pregnant rats were gavaged with vehicle, EE2 (0.05-50 microg/kg/day), or BPA (2, 20, and 200 microg/kg/day) from day 7 of gestation to postnatal day (PND) 18, and the female offspring were studied. EE2 (50 microg/kg/day) increased anogenital distance and reduced pup body weight at PND2, accelerated the age at vaginal opening, reduced F1 fertility and F2 litter sizes, and induced malformations of the external genitalia (5 microg/kg). F1 females exposed to EE2 also displayed a reduced (male-like) saccharin preference (5 microg/kg) and absence of lordosis behavior (15 microg/kg), indications of defeminization of the CNS. BPA had no effect on any of the aforementioned measures. These results demonstrate that developmental exposure to pharmacologically relevant dosage levels of EE2 can permanently disrupt the reproductive morphology and function of the female rat.
Lazy home migration for distributed shared memory systems (2002)

In a distributed shared memory system, each memory page is associated with a home node that maintains the directory state for cache lines within that page. Memory access patterns and home node locations have a strong influence on performance, especially if remote communication is costly. Since access patterns are difficult to predict and may change dynamically, it is useful to dynamically migrate home nodes to reduce the amount of remote communication. The paper presents a new and efficient algorithm for migrating home nodes in distributed shared memory systems. Unlike previous page migration algorithms, our algorithm avoids global coordination. Allowing the system to be more responsive to changing workloads. We verify the algorithm's correctness with the Mur/spl sigma/ protocol verification tool. We explore several policies for deciding when and where to migrate home nodes. Trace driven simulations of several SPLASH-2 benchmarks show that our home migration algorithm and policies can reduce the amount of remote communication by 50%. The results also emphasize the importance of minimizing the cost of migration.
Interferometrische Bestimmung der Funduspulsation des menschlichen Auges (2009)

In der vorliegenden Dissertation wurde ein prototypisches Messverfahren realisiert, welches aus interferometrischen Messdaten die Funduspulsation automatisch bestimmt. Es wurden Algorithmen entwickelt, welche aus den gemessenen Interferogrammen die Funduspulsation bestimmen und dabei robust gegen auftretende Speckle sind.
Le répulsif IR3535, agent synergisant potentiel d’un insecticide non-pyréthrinoide dans la lutte contre les moustiques vecteurs de maladies (2014)

National audience
Modeling COVID‐19: Forecasting and analyzing the dynamics of the outbreaks in Hubei and Turkey (2022)

As the pandemic of Coronavirus Disease 2019 (COVID‐19) rages worldwide, accurate modeling of the dynamics thereof is essential. However, since the availability and quality of data vary dramatically from region to region, accurate modeling directly from a global perspective is difficult. Nevertheless, via local data collected by certain regions, it is possible to develop accurate local prediction tools, which may be coupled to develop global models. In this study, we analyze the dynamics of local outbreaks of COVID‐19 via a system of ordinary differential equations (ODEs). Utilizing a large amount of data available from the ebbing outbreak in Hubei, China, as a testbed, we predict the trajectory of daily cases, daily deaths, and other features of the Hubei outbreak. Through numerical experiments, we observe the effects of social distancing on the dynamics of the outbreak. Using knowledge gleaned from the Hubei outbreak, we apply our model to analyze the dynamics of the outbreak in Turkey. We provide forecasts for the peak of the outbreak and the daily number of cases and deaths in Turkey, by varying levels of social distancing and the transition rate which is from infected class to confirmed infected class.
Cystatin C– and Creatinine-Based Estimates of Renal Function and Their Value for Risk Prediction in Patients with Acute Coronary Syndrome: Results from the PLATelet Inhibition and Patient Outcomes (PLATO) Study (2013)

Despite differences in cystatin C concentrations, overall correlation between the Gentian and Roche assays was good, while agreement was moderate. The combined creatinine-cystatin C equation did not outperform risk prediction by CKD-EPI.
Convenient Synthesis of Cyclohexa[a]pyrrolo[2,1-b][3]benzazepine, a Cephalotaxus Alkaloid Analogue (1999)

Irradiation of N-(3-hydroxy-4-methoxyphenethyl)-3-(2-iodo-5methoxyphenyl)propionamide (11) in methanol in the presence of sodium hydroxide furnished 3-hydroxy-2,12-dimethoxy-6,7,9.1C-tetrahydrodiknz-[d,flazecin-8(5H)-one(14) which was successfully led to cephalotaxine analogue (17) bearing py&olo[2,1-b][3]benzazepine skeleton by reduction with borane followed by Birch reduction and subsequent acidic treatment.The structure of 17 was established by means of X-Ray crystallography.We previously reponed that bromophenolic amides underwent photochemical cyclization reaction to form easily diknz[d,flazecine skeleton,'.' which was subjected to reduction with horane followed by Birch reduction and subsequent acidic treatment to give homoerythrinan derivative?The dibenz[d,flazecine base (4) might be the important intermediate in the biosynthesis of homoerythrinan alkaloids and cephalotaxan alkaloids' (Scheme 1) whose estef derivatives, harringtonine (2) and homohamingtonine (3), have potent antileukemic activity.'From view of spectrum of the biological activity and unique structural features, there are many reports on the synthesis of cephalotaxine (1)' and its analogues, homocephalotaxine6 and aza-cephalotaxine derivative,' whose pivotal skeleton is pyrrolo[2,1b][3]henzazepine.In this paper, we wish to describe a novel route for the synthesis of a cephalotaxine analogue (17) having the pyrrolo[2,1-bl[3]benzazepine skeleton from a dibenz[d,flazecine base (15) using Birch reduction followed by acidic treatment.First, the dibenz[d,flazecine base (15) was prepared as shown in Scheme 2. The condensation reaction of P-(2-iodo-5-methoxyphenyl)propionic acid (8)' and 3-benzyloxy-4-methoxyphenethylamine (9) in refluxing xylene gave 10 in 73% yield.Treatment of 10 with methanesulfonic acid and thioanisole in
Free Vascularized Fibular Graft Reconstruction of Large Skeletal Defects after Tumor Resection (2009)

Level IV, case series. See Guidelines for Authors for a complete description of levels of evidence.