gebhart/patrepeval huggingface dataset:
Na-K activated ATPase and the release of acetylcholine and noradrenaline. (1982)

1. It has been shown that different experimental conditions known to inhibit Na-K-activated ATPase, and enzyme present in the neuronal membranes, are able to promote transmitter release (ACh, NA, etc.) from different tissues, simply by making the membrane leaky. 2. Under physiological conditions, Ca entering the cell transiently inhibits membrane ATPase, resulting in a transient change in membrane permeability and a subsequent release of transmitter. 3. When membrane ATPase inhibitor was used one part of the release proved to be Ca-independent. This finding indicates that the voltage and Ca-dependent link of transmitter release can be by-passed by direct membrane ATPase inhibitors (ouabain). 4. Neurochemical and electrophysiological evidence was obtained on mouse diaphragm that most of the released ACh is cytoplasmic and Na-K ATPase inhibition is responsible for its release. 5. The stimulation of membrane ATPase (by switching off K and its readmission) results in an inhibition of both ACh and noradrenaline release evoked by axonal stimulation. 6. It is suggested that, in those cases where the varicose axon terminals do not make synaptic contact, the transmitter released from the cytoplasmic pool contributes to the transmission, since during diffusion (sometimes few thousand nm) transmitter of different origins becomes mixed up.
DISC: dynamic instruction stream computer-an evaluation of performance (2002)

DISC is a simple processor architecture targeted for real-time applications. The architecture is based on dynamic fine-grained multithreading where the next instruction is fetched from one of several possible simultaneously active threads. The DISC architecture uses a combination of concepts including, a register stack file, a four stage pipeline, up to four active threads, a dynamic scheduler, and special input/output (I/O) and interrupt constructs to allow maximization of performance for real-time control applications. Previous stochastic results were very encouraging and so a synthetic benchmark was developed to allow more detailed testing. The benchmark was based on a Hughes Aircraft Company satellite control system, and assembled with the DISC assembler. The model was designed and run in the Verilog simulation language.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Injectable Mesenchymal Stem Cell Therapy for Large Cartilage Defects—A Porcine Model (2007)

Current techniques in biological resurfacing of cartilage defects require an open arthrotomy or arthroscopy and involve the direct transplantation of isolated cells and/or scaffolds or whole tissue grafts with chondrogenic potential onto the cartilage defect. Our study investigates the possibility of direct intra-articular injection of mesenchymal stem cells suspended in hyaluronic acid (HA) as an alternative to the much more invasive methods currently available. A partial-thickness (without penetration of the subchondral bone) cartilage defect was created in the medial femoral condyle of an adult minipig. Mesenchymal stem cells from the iliac crest marrow of the same pig harvested in a separate procedure and suspended in 2 milliliters of hylan G-F 20 (Synvisc) were injected intra-articularly after the creation of the defect. This was followed by two more injections of hylan G-F 20 (HA) at weekly intervals. Either saline or HA was injected into the knees of the controls. The pigs were sacrificed at 6 and 12 weeks for morphological and histological analysis. The cell-treated groups showed improved cartilage healing both histologically and morphologically at 6 and 12 weeks compared with both controls. The use of intra-articular injections of mesenchymal stem cells suspended in HA is a viable option for treating large cartilage defects. This would be further explored in clinical trials.
Non-Data-Aided Symbol Rate Estimation of Linearly Modulated Signals (2008)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> The estimation of the symbol rate of a linearly modulated signal is addressed, with special focus on low signal-to-noise ratio (SNR) scenarios. This problem finds application in automatic modulation classification and signal monitoring. A maximum-likelihood (ML) approach is adopted to derive practical estimators, exploiting information on the cyclostationarity of the modulated signal as well as knowledge of the received signaling pulse shape. The structure of the ML estimator suggests a two-step estimation procedure, whereby an initial coarse search determines first a neighborhood from which a subsequent fine search yields the final symbol rate estimate. Links between the ML approach and previous results from the literature in symbol rate estimation are established as well. The proposed scheme is applicable even for small excess bandwidths, at the cost of a higher complexity with respect to simpler estimators known to fail under such conditions. </para>
Biomimetic Reduction of Sulfuric Acid (1983)

Abstract Sulfuric acid and sodium sulfate were readily reduced to elemental sulfur and hydrogen sulfide upon treatment with a mixture of either one of polyphosphoric acid derivatives, PPE, PPA, and P4O10, which can form a mixed anhydride having –P–O–S– linkage, and iodide or thiol. Sulfur dioxide, which is undoubtedly one of important intermediates, was trapped by p-tolyllithium to afford p-toluenesulfinic acid which was converted to p-tolyl methyl sulfone upon treatment with methyl iodide, though the yield was low. Sulfur trioxide which has been postulated as the key intermediate in the biological reduction of inorganic sulfate, was also trapped by mesitylene to give mesitylenesulfonic acid in a high yield. The reduction of sulfate to elemental sulfur and hydrogen sulfide is considered to proceed through the course which resembles the biological reaction path involved in the assimilatory metabolism of inorganic sulfate in microorganisms and plants.
ChemInform Abstract: Selenoxide Elimination and [2,3]‐Sigmatropic Rearrangement (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Responsible Science, Engineering and Education for Water Resource Recovery and Circularity (2020)

Water resource recovery is central to the circular economy framework. It underlies the transition of environmental engineering from pollution prevention to responsible innovation for sustainable systems engineering. In order to speed this transition, resource recovery and circularity need integration into new higher education curricula to train the next generation of young professionals. However, training of new concepts requires the development of new course materials and books, while integrating substantial illustrations and problems on circularity and resource recovery in new editions of existing textbooks in environmental science and engineering. Moreover, university-utility-industry partnerships are important mechanisms to bridge theoretical fundamentals to concepts for engineering practice, and to promote knowledge exchange and technology adoption between practitioners and academics. Interactive platforms should be designed to facilitate the integration and development of resource recovery and circularity concepts from science and practice into education. This paper gives actionable roadmaps to (i) apprehend how new science and technological findings need to get integrated to sustain resource recovery and circularity in practice. It highlights that (ii) skills sets can be engineered with relatively minor changes to existing lecture material that will have maximal impact on the scope of the thought material. It lays out (iii) how partnership with engineering practitioners can make a lecture more vivid by giving students reasoning for why the learned material is important. It drives (iv) a platform for an integrated science, education and practice to deliver them with concrete tools for practical implementation for benefits at community level.
Efficient target-response interpolation for a graphic equalizer (2016)

A graphic equalizer is an adjustable filter in which the command gain of each frequency band is practically independent of the gains of other bands. Designing a graphic equalizer with a high precision requires evaluating a target response that interpolates the magnitude response at several frequency points between the command gains. Good accuracy has been previously achieved by using polynomial interpolation methods such as cubic Hermite or spline interpolation. However, these methods require large computational resources, which is a limitation in real-time applications. This paper proposes an efficient way of computing the target response without sacrificing the approximation accuracy. This new approach called Linear Interpolation with Constant Segments (LICS) reduces the computing time of the target response by 55% and has an intrinsic parallel structure. Performance of the LICS method is assessed on an ARM Cortex-A7 core, which is commonly used in embedded systems.
Adrenocortical cytochrome P-450 side chain cleavage. Preparation of membrane-bound side chain cleavage system from purified components. (1979)

Soluble cytochrome P-450 from bovine adrenocortical mitochrondria, capable of side chain cleavage, can be incorporated into membranes prepared by dispersion of phospholipids in aqueous buffer when cholate is added to the membrane suspension. In addition, the complete protein side chain cleavae system (i.e. including the ancillary proteins adrenodoxin and adrenodoxin reductase and the substrate cholesterol) can be incorporated into such membranes so that on addition of TPNH, pregnenolone is formed. These components remain in the membrane through gel filtration (which removes almost all the cholate) and sedimentation through sucrose density gradients which separate vesicles without protein and soluble enzyme from the membrane-bound P-450 remains associated with the membrane during and following lysis of vesicles. The vesicles which do not leak [14C]glucose were seen on electron microscopy to show a mean diameter of 350 to 450 A. A number of phospholipids are capable of accomodating P-450 in this manner: mitochondrial lipid extracts, synthetic dipalmitoyl phosphatidylcholine, synthetic dipalmitoyl phosphatidylserine, and egg lecithin, separately or in various combinations. Cholesterol is not necessary for incorporation of the side chain cleavage system. Membrane-bound P-450 shows a Vmax of 28.1 nmol of pregnenolone/min/mg of protein, more than 10 times that of soluble P-450. The spectral properties of the soluble P-450 are altered to become predominantly low spin in the membrane and the enzyme is more stable at 4 degrees C than is soluble p-450.
T cell reactivity to regulatory factor X4 in type 1 narcolepsy (2021)

Type 1 narcolepsy is strongly (98%) associated with human leukocyte antigen (HLA) class II DQA1*01:02/DQB1*06:02 (DQ0602) and highly associated with T cell receptor (TCR) alpha locus polymorphism as well as other immune regulatory loci. Increased incidence of narcolepsy was detected following the 2009 H1N1 pandemic and linked to Pandemrix vaccination, strongly supporting that narcolepsy is an autoimmune disorder. Although recent results suggest CD4+ T cell reactivity to neuropeptide hypocretin/orexin and cross-reactive flu peptide is involved, identification of other autoantigens has remained elusive. Here we study whether autoimmunity directed against Regulatory Factor X4 (RFX4), a protein co-localized with hypocretin, is involved in some cases of narcolepsy. Studying human serum, we found that autoantibodies against RFX4 were rare. Using RFX4 peptides bound to DQ0602 tetramers, antigen RFX4-86, -95, and -60 specific human CD4+ T cells were detected in 4/10 patients and 2 unaffected siblings, but not in others. Following culture with each cognate peptide, enriched autoreactive TCRαβ clones were isolated by single-cell sorting and TCR sequenced. Homologous clones bearing TRBV4-2 and recognizing RFX4-86 in patients and one twin control of patient were identified. These results suggest the involvement of RFX4 CD4+ T cell autoreactivity in some cases of narcolepsy, but also in healthy donors.
The BET-bromodomain inhibitor JQ1 renders neuroblastoma cells more resistant to NK cell-mediated recognition and killing by downregulating ligands for NKG2D and DNAM-1 receptors (2019)

Low expression of ligands for NK cell-activating receptors contributes to neuroblastoma (NB) aggressiveness. Recently, we demonstrated that the expression of MYCN, a poor prognosis marker in NB, inversely correlates with that of activating ligands. This indicates that MYCN expression level can predict the susceptibility of NB cells to NK cell-mediated immunotherapy and that its downregulation can be exploited as a novel therapeutic strategy to induce the expression of activating ligands. Here we evaluated the effect of the BET-bromodomain inhibitor JQ1 on the expression of ligands for NK cell-activating receptors in NB cell lines. Although downmodulating MYCN, JQ1 impaired the expression of ligands for NK cell-activating receptors, rendering NB cell lines more resistant to NK cell-mediated killing. The downregulation of activating ligands was due to JQ1-mediated impaired functions of both c-MYC and p53, two transcription factors known to regulate the expression of ULBP1-3 ligands for NKG2D activating receptor. Moreover JQ1 strongly downregulated the levels of ROS, a stress-induced signaling event associated with the induction of ligands for NK cell-activating receptors. These results suggest that the use of JQ1 should be discourage in combination with NK cell-based immunotherapy in a perspective chemotherapeutic treatment of NB. Thus, further investigations, exploiting molecular strategies aimed to boost the NK cell-mediated killing of NB cells, are warranted.
Influence of Processing Conditions on Melt Blown Web Structure. Part III — Water Quench (2005)

The overall goal of our recent research has been to quantitatively measure the influence of commercial processing variables on the structure of polypropylene melt blown webs. In this paper, we applied a cool water quench spray to fibers to increase their thermal quenching rate. Then, we collected webs and measured single fiber diameter, shot content, fiber bundle size, basis weight uniformity and pore structure. The structure of webs produced with and without quenching was compared to access the influence of quenching on webs.
LiMnPO4 Olivine as a Cathode for Lithium Batteries (2011)

The olivine structured mixed lithium-transition metal phosphates LiMPO4 (M = Fe, Mn, Co) have attracted tremendous attention of many research teams worldwide as a promising cathode materials for lithium batteries. Among them, lithium manganese phosphate LiMnPO 4 is the most promising considering its high theoretical capacity and operating voltage, low cost and environmental safety. Various techniques were applied to prepare this perspective cathode for lithium batteries. The solution based synthetic routes such as spray pyrolysis, precipitation, sol-gel, hydrothermal and polyol synthesis allow preparing nanostructured powders of LiMnPO 4 with enhanced electrochemical properties, which is mostly attributed to the higher chemical homogeneity and narrow particle size distribution of the material. Up-to-date, the LiMnPO4/C composites prepared by the spray pyrolysis route have the best electrochemical performance among the reported in the literature.
Simultaneous Heat and Moisture Transport in 3D Printed Walls (2022)

The national and international building stock -representing one of the most intensive energy-consuming sectors worldwide -is characterized by a large share of old constructions, designed without following any energy criteria. This scenario has promoted the rising of powerful technologies, e.g., Additive Manufacturing (AM), which despite its recent rise is leading the innovation process involving both the industrial and civil sectors. 3D printing techniques are going to outperform current production techniques because of their various advantages, i.e., design of complex forms, uniform materials, reduced production steps and costs. The aim of the present work is to combine the accuracy of computer-aided design (CAD) for AM structures with the benefits of the computational thermo-fluid dynamic simulation (CFD) to perform thermal and moisture performance analysis of innovative building walls. Natural convection and radiation problem -involving buoyancy-driven flow in a cavity -is investigated and solved under appropriate boundary conditions defined in a finite element commercial code. After validation with international guidelines and literature data, the model is simulated in Napoli (Italy) under winter design conditions. Moreover, this work provides a comparison between a simplified procedure for the condensation risk detection, i.e., the Glaser method, and an advanced one -based on the steady-state diffusion theory -which considers latent heat effect and capillary transport of moisture liquid. The results show that the radiative heat transfer mechanism has a significant influence on thermal transmittance. On the other hand -with reference to the case study -here we present the discrepancy between the prediction of the condensation effect during the winter months by adopting the present method with respect to the Glaser one.
Bankcheck Recognition using Cross Validation Between Legal and Courtesy Amounts (1997)

A bankcheck reading system using cross validation of both the legal and the courtesy amounts is presented in this paper. Some of the challenges posed by the task are (i) segmentation of the legal amount into words, (ii) location of boundaries between dollars and cents amounts, and (iii) high accuracy in terms of recognition performance. Word segmentation in the legal amount is a serious issue because of the nature of the data and patrons' writing habits which tend to clump words together. We have developed a word segmentation algorithm based on the character segmentation results to address this issue. The list of possible amounts generated by the word segmentation hypotheses is used as lexicon for the courtesy amount recognition. The order of magnitude of the amount is estimated during legal amount recognition. We treat the courtesy amount as a numeral string and apply the same word recognition scheme as used for the legal amount. Our approach to check recognition differs from traditional methods in two significant aspects: First, our emphasis on both the legal and the courtesy amounts is balanced. We use an accurate word recognizer which performs equally well on alpha words and digit strings. Second, our combination strategy is serial rather than the commonly used parallel method. Experimental results show that 43.8% of check images are correctly read with an error rate of 0%.
Electron scattering from Li at 5.4, 10, 20 and 60 eV impact energies (1976)

Differential and integral cross sections for elastic scattering and for the excitation of the 2p2P, 3p2P, 4p2P and 3s2S states of lithium have been determined and elastic momentum transfer cross sections have been obtained at 5.4, 10 and 60 eV electron impact energies. The cross sections were normalized to the absolute scale by using the total cross section measurements of Kasdan et al. (1971). The integral elastic scattering cross sections have been compared to calculations utilizing the Glauber and Born approximations and the 2p2P integral cross section results have been compared with optical excitation function measurements and with close-coupling calculations.
Names should mean what, not where (1992)

Naming is a fundamental issue in distributed systems that is growing in importance as the number of directly accessible systems and resources grows to the point that it is difficult to discover the names of files or objects of interest. Based upon our recent experience building a system that combines the ideas of location and naming we propose a naming architecture called content names that is a departure from a traditional tree structured hierarchy. In a content name system a name can include a description of what a user seeks in addition to where an interesting file or object might be found. Our experience with an implementation of content names in a Semantic File System suggests that they will be widely applicable to future distributed system architectures. In addition to our own experience, we have observed a trend towards content names in new distributed services, such as the Archie system and the Automount daemon. We discuss how a Semantic File System can be used to implement content names and examine its feasibility in the context of a USENET publishing system.
Interfacing Boron Doped Diamond and Biology: An Insight on Its Use for Bioanalytical Applications (2005)

Abstract Boron doped diamond (BDD) films are promising materials for electroanalysis and bioelectroanalysis. This contribution gives a short review on BDD functionalization by chemical and biochemical entities and on the applications of BDD electrodes to electroanalysis of chemical compounds of biological interest. This review is illustrated by the developments on BDD interfaces in both these areas carried out in our group. BDD electrodes were prepared by means of microwave plasma‐assisted CVD onto silicon substrate giving rise to H‐terminated BDD films. Anodic polarization of BDD electrodes in aqueous media generates hydrophilic oxidized surfaces and extended electrochemical window in aqueous media. We took advantage of this enlarged electrochemical window together with the hydroxylated surface for the functionalization of BDD electrode surfaces with biomolecules and for bioelectroanalysis. We have designed a novel route of BDD functionalization using OH terminations of the anodized surface. The modification of the BDD surface with biotin groups will be presented here as biologically active model. Furthermore, as the anodic potential window of BDD increases upon electrochemical activation, 2′‐deoxyguanosine (1.2 V vs. Ag/AgCl) and 2′‐deoxyadenosine (1.5 V vs. Ag/AgCl) could be detected electrochemically with an acceptable signal to noise ratio. The electrochemical signature of each oxidizable base was assessed using differential pulse voltammetry (DPV). These experiments pointed towards adsorption of the oxidized products, which were investigated macroscopically by DPV and at the microscopic level by SECM (scanning electrochemical microscopy).
Destabilization of potato spindle tuber viroid by mutations in the left terminal loop. (1997)

Infectivity studies with highly infectious RNA inocula generated by ribozyme cleavage were used to compare the biological properties of three apparently nonviable mutants of potato spindle tuber viroid (PSTVd). One of these mutants (PSTVd-P) contains three nucleotide substitutions in the left terminal loop, and mechanical inoculation of tomato seedlings with RNA transcripts at levels equivalent to 10(3)-10(5) times the ID50 for PSTVd-Intermediate failed to result in systemic infection. Viable progeny containing a spontaneous C-->G change at position 4 could, however, be recovered from transgenic Nicotiana benthamiana plants that constitutively expressed PSTVd-P RNA. The initial mutations in PSTVd-P led to an overall weakening of its native structure in vitro, and the precisely-full-length molecule released by ribozyme cleavage in vivo was also unstable. Even RT-PCR analysis failed to reveal detectable amounts of circularized PSTVd-P among the RNAs isolated from uninfected plants. Predicted stabilizing effects of a spontaneous mutation at position 4 suggest that the appearance of viable progeny was dependent on a combination of events: errors by host RNA polymerase II during transcription of the mutant transgene coupled with a strong selective pressure against alterations in the native structure of PSTVd.