gebhart/patrepeval huggingface dataset:
Forskolin, phosphodiesterase inhibitors, and cyclic AMP analogs inhibit proliferation of cultured bovine aortic endothelial cells (1986)

The role of cyclic AMP on endothelial cell proliferation was investigated, since these cells can be exposed to high concentrations of physiological and pharmacological agents that alter cyclic AMP metabolism. Cloned bovine aortic endothelial cells were plated at 25,000 cells/35mm dish and grown for 5 days in the presence of phosphodiesterase (PDE) inhibitors, forskolin, or cyclic AMP analogs. The PDE inhibitors dipyridamole, ZK 62 711, isobutylmethylxanthine (IBMX) and theophylline inhibited cell growth in a concentration-dependent manner. Dipyridamole produced a 30% and a 50% inhibition at 5 microM and 12.5 microM, while higher concentrations were cytotoxic. At its therapeutic plasma concentration range (50-100 microM) theophylline inhibited cell proliferation by 15-25%, while IBMX and the highly specific cyclic AMP phosphodiesterase inhibitor, ZK 62 711 inhibited growth by 60-80% and 40-50%, respectively. Forskolin (5 microM) increased cyclic AMP levels and cyclic AMP-kinase activity ratios by 2.5-fold and 2-fold. In the absence of PDE inhibitors forskolin produced a 20% growth inhibition at 0.5 microM and a 60% inhibition at 10 microM. The forskolin dose-response curve was not altered by theophylline, but was shifted to the left by approximately 10-fold with dipyridamole and ZK 62 711 and 5-fold with IBMX. Forskolin (5 microM), by itself produced a 1.8-fold increase in cyclic AMP. In the presence of 5 microM theophylline, dipyridamole, IBMX, and ZK 62 711, cyclic AMP was increased by forskolin 2.0, 2.6, 3.5, and 6.6-fold, respectively. 8-Bromo cyclic AMP and dibutyryl cyclic AMP produced a 55% and 60% growth inhibition at 100 microM. The cyclic GMP analogs were less effective inhibitors of growth (15-30%). Our results demonstrate that cyclic AMP analogs and pharmacological agents that elevate intracellular cyclic AMP levels inhibit cell growth and suggest that cyclic AMP may be an important endogenous regulator of endothelial cell proliferation.
Outil d'estimation de la distribution complète des cotes de retenue atteintes en crue pour un barrage capacitif (2019)

Dans le cadre des estimations de crues extrêmes, les hydrologues d'EDF génèrent à l'aide de la méthode SCHADEX des centaines de milliers de crues synthétiques de toutes intensités avec des dynamiques très variées. Cet article détaille l'ensemble du travail réalisé par les hydrauliciens d'EDF pour le développement d'un outil performant, dédié au traitement de ces scénarios hydrologiques. Cet outil, nommé MELχOR, est capable de simuler le passage de 200 000 hydrogrammes dans un barrage capacitif en 2 à 3 heures de calcul. La sûreté de l'ouvrage vis-à-vis du risque de crue est finalement étudiée de manière plus robuste par le biais de la distribution de la cote maximale atteinte lors de ces crues.
Rapid conversion of glutamate to tricarboxylic acid cycle intermediates during the initial period of moderate intensity exercise. (1995)

Dept. of Human Biology, University of Limburg, The Netherlands; #Dept. of Physiology and Pharmacology, Karolinska Institute, Sweden.
Distributed optical fibre sensing in synthetic fibre ropes and cables (1999)

Distributed strain measurements on synthetic fibre ropes as used in marine applications are reported. The ropes incorporate single mode fibre-optic sensors for strain measurement. A Brillouin-amplification-based distributed strain measuring system has been utilised to interrogate the fibre sensors incorporated into a parallel yarn aramid rope. Initial results are presented to conclusively demonstrate the technical feasibility ofthe approach.
An $RC$ Plasma Device for Sterilization of Root Canal of Teeth (2009)

The application of cold plasma in sterilization of a root canal of a tooth has recently attracted great attention. In this paper, a reliable and user-friendly plasma-jet device, which can generate plasma inside the root canal, is reported. The plasma can be touched by bare hands and can be directed manually by a user to place it into root canal for disinfection without causing any painful sensation. When He/O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> (20%) is used as working gas, the rotational and vibrational temperatures of the plasma are about 300 K and 2700 K, respectively. The peak discharge current is about 10 mA. Preliminary inactivation experiment results show that it can efficiently kill <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">enterococcus</i> <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">faecalis</i> , one of the main types of bacterium causing failure of root-canal treatment in several minutes.
FUNCTIONAL COATING OF HETEROGENEOUS MICROSTRUCTURE SURFACES WITH SELF INTERACTING BIOMOLECULES (2010)

This paper presents useful techniques for applying coatings of the extracellular matrix (ECM) proteins fibronectin (FN) and basement membrane extract (BME) to small, defined polymeric microstructures. We found that several cell types, such as fibroblastic and epithelial, have preferential adherence to these ECM component coatings, but that the coatings were ineffective when applied using standard coating protocols. Thus we developed strategies to effectively apply FN and BME, overcoming inherent difficulties with their use in applications such as the micropallet array technology. These results would be of consequence for numerous bioMEMS devices that involve cell interaction with similar microstructured polymer surfaces.
Listen to Your Heart: Evaluation of the Cardiologic Ecosystem. (2021)

Modern implantable cardiologic devices communicate via radio frequency techniques and nearby gateways to a backend server on the internet. Those implanted devices, gateways, and servers form an ecosystem of proprietary hardware and protocols that process sensitive medical data and is often vital for patients’ health. This paper analyzes the security of this Ecosystem, from technical gateway aspects, via the programmer, to configure the implanted device, up to the processing of personal medical data from large cardiological device producers. Based on a real-world attacker model, we evaluated different devices and found several severe vulnerabilities. Furthermore, we could purchase a fully functional programmer for implantable cardiological devices, allowing us to re-program such devices or even induce electric shocks on untampered implanted devices. Additionally, we sent several Art. 15 and Art. 20 GDPR inquiries to manufacturers of implantable cardiologic devices, revealing non-conforming processes and a lack of awareness about patients’ rights and companies’ obligations. This, and the fact that many vulnerabilities are still to be found after many vulnerability disclosures in recent years, present a worrying security state of the whole ecosystem.
Kinetics of crystallization of isotactic polypropylene between 120 and 160°C. (1959)

Abstract Physical changes which accompany the crystallization of isotactic polypropylenes have been observed by dilatometry and optical microscopy at temperatures between 120 and 160 ° C. Samples were fused at 220 ° C. and then held at a predetermined crystallization temperature for times ranging up to many hours. Rate constants for the crystallization were calculated from volume changes and from measurements of radial growth of spherulites. Polypropylenes from different sources exhibited quite different concentrations of spherulites and growth rates. Crystallization appears to proceed, at least at the higher temperatures, by spherical growth from a fixed number of nuclei, apparently arising from foreign matter in the polymer. With decreasing temperature, the number of nuclei (hence also the number of spherulites) increases, since apparently the conditions for nucleation become less critical. The crystallization follows the kinetics of a nucleation‐controlled process according to an Avrami equation, ‐log (1 ‐ Δ V / Δ V ∞) = kt 3 . Deviations from the third power of time at the lower temperatures may be the result of a secondary ordering process, slower than the primary crystallization.
Compositional Verification Methods for Next-Generation Concurrency (Dagstuhl Seminar 15191) (2015)

This report documents the program and the outcomes of Dagstuhl Seminar 15191 "Compositional Verification Methods for Next-Generation Concurrency". The seminar was successful and facilitated a stimulating interchange between the theory and practice of concurrent programming, and thereby laid the ground for the development of compositional verification methods that can scale to handle the realities of next-generation concurrency.
A Novel Class of SINE Elements Derived from 5S rRNA (2003)

Eukaryotic genomes are colonized by different retroposons, including short interspersed repetitive elements (SINEs). All currently known SINEs are derived from tRNA and 7SL RNA genes and exploit their type 2 internal pol III promoters. We report here a novel class of SINE elements, called SINE3, derived from 5S rRNA. SINE3s are transcribed from the type 1 internal pol III promoter. Approximately 10,000 copies of SINE3 elements are present in the zebrafish genome, they constitute approximately 0.4% of the genomic DNA. Some elements are as little as 1% diverged from each other, indicating that the retrotransposition of SINE3 in zebrafish is an ongoing process. The 3'-tail of SINE3 is significantly similar to that of CR1-like non-LTR retrotransposons, represented by numerous subfamilies in the zebrafish genome. Analogously to CR1-like elements, SINE3 copies are not flanked by target site duplications, and their 3' termini are composed of (ACATT)n and (ATT)n microsatellites, specific for different subfamilies of SINE3. Given the common structural features, it is highly likely that the enzymatic machinery encoded by CR1-like elements powers proliferation of SINE3.
Two Vision-guided vehicles: temporal coordination using nonlinear dynamical systems (2007)

This article addresses the problem of generating timed trajectories and temporally coordinated movements for two wheeled vehicles, when relatively low-level, noisy sensorial information is used to steer action.The generated trajectories have controlled and stable timing (limit cycle type solutions).Incoupling of sensory information enables sensor driven termination of movement.We build on a previously proposed solution in which timed trajectories and sequences of movements were generated as attractor solutions of dynamic systems.We present a novel system composed of two coupled dynamical architectures that temporally coordinate the solutions of these dynamical systems.The coupled dynamics enable synchronization of the different components providing an independence relatively to the specification of their individual parameters.We apply this architecture to generate temporally coordinated trajectories for two vision-guided mobile robots in a non-structured simulated environment, whose goal is to reach a target within a certain time independently of the environment configuration or the distance to the target.The results illustrate the robustness of the proposed decision-making mechanism and show that the two vehicles are temporal coordinated: if a robot movement is affected by the environment configuration such that it will take longer to reach the target, the control level coordinates the two robots such that they terminate approximately simultaneously.
High-Level Expression of Escherichia coli tRNA (m 5 U54)-Methyltransferase (1990)

A cloning and high-expression system for tRNA (m5U54)-methyltransferase (RUMT) is described. Polymerase chain reaction (PCR) was used to replicate the coding sequence and create flanking restriction sites for cloning. The PCR product was then inserted into expression vectors containing the tac and PL promoters. With the PL promoter, induced cells produced about 1.5% of their soluble protein as catalytically active RUMT. With the tac promoter, up to 8% of the total cell protein was active enzyme, and RUMT was purified to near homogeneity in three steps.
Verification of web content integrity : a new approach to protect servers against tampering (2007)

The provision of web services is a real-time process, conducted in ad-hoc, ‘off the cuff’ manner. Consequently the verification of the data content and the identification of any authorized data interference or manipulation are not without problems. Some progress has been made in addressing the verification of server content integrity, but current solutions are restricted by the limitations of the SSL protocol, the statelessness of HTTP, and difficulties with automatic code analysis. This paper reviews the problems associated with unauthorized data manipulation of static and dynamic web content, presents a web security real-time framework that can be used to verify the static and dynamic web content of a requested page. It is suggested that such a framework will offer an increased level of user confidence, since the framework will provide a much greater protection against web server subversion.
WorkWeb: Enhancing Collaboration and Communication in Community Based Clinical Research through Innovative Uses of Wikis (2007)

The NIH Roadmap Initiative aims to re-engineer the clinical research enterprise in the United States. The Inter-Trial project at Columbia University is re-engineering clinical research in community practice settings by improving collaboration and communication. Our intervention, informed by numerous studies of needs and workflow, is an online, collaborative space, named the WorkWeb that brings together many different stakeholders (such as clinical research coordinators [CRCs] and investigators) and resources to facilitate clinical trials. The WorkWeb includes tools to support several research tasks and allows stakeholders to create peer-to-peer support groups. The WorkWeb has been built entirely using the wiki philosophy and thus represents a paradigm shift in providing its end-users almost full control over the content and features.
Delayed Adaptation of the Heart to Stress (2004)

The early phase of preconditioning (PC) lasts 2 to 3 hours and protects against myocardial infarction, but not against stunning. In contrast, the late phase of PC lasts for 3 to 4 days and protects against both myocardial stunning and infarction, making this phenomenon more clinically relevant. Late PC is a genetic reprogramming of the heart that involves the activation of several stress-responsive genes, which ultimately results in the development of a cardioprotective phenotype. Sublethal ischemic insults release chemical signals (nitric oxide [NO], adenosine, and reactive oxygen species) that trigger a series of signaling events (eg, activation of protein kinase C, Src protein tyrosine kinases, Janus kinases 1/2, and nuclear factor-kappaB) and culminates in increased synthesis of inducible NO synthase, cyclooxygenase-2, heme oxygenase-1, aldose reductase, Mn superoxide dismutase, and probably other cardioprotective proteins. In addition to ischemia, heat stress, exercise, and cytokines can also induce a similar series of events. Perhaps most importantly, many pharmacologic agents (eg, NO donors, adenosine receptor agonists, endotoxin derivatives, or opioid receptor agonists) can mimic the effects of ischemia in inducing the late phase of PC, suggesting that this phenomenon might be exploited therapeutically. The purpose of this review is to summarize the mechanisms that underlie the late phase of ischemic PC.
Multi-Column Xe/Kr Separation with AgZ-PAN and HZ-PAN (2016)

Previous multi-column xenon/krypton separation tests have demonstrated the capability of separating xenon from krypton in a mixed gas feed stream. The results of this initial testing with AgZ-PAN and HZ-PAN indicated that an excellent separation of xenon from krypton could be achieved. Building upon these initial results, a series of additional multi-column testing were performed in FY-16. The purpose of this testing was to scale up the sorbent beds, test a different composition of feed gas and attempt to improve the accuracy of the analysis of the individual capture columns’ compositions. Two Stirling coolers were installed in series to perform this testing. The use of the coolers instead of the cryostat provided two desired improvements, 1) removal of the large dilution due to the internal volume of the cryostat adsorption chamber, and 2) ability to increase the sorbent bed size for scale-up. The AgZ-PAN sorbent, due to its xenon selectivity, was loaded in the first column to capture the xenon while allowing the krypton to flow through and be routed to a second column containing the HZ-PAN for capture and analysis. The gases captured on both columns were sampled with evacuated sample bombs and subsequently analyzed via GC-MS for both krypton and xenon. The results of these tests can be used to develop the scope of future testing and analysis using this test bed for demonstrating the capture and separation of xenon and krypton using sorbents, for demonstrating desorption and regeneration of the sorbents, and for determining compositions of the desorbed gases. They indicate a need for future desorption studies in order to better quantify co-adsorbed species and final krypton purity.
Stereoselective synthesis of cis 2,3-disubstituted cycloheptanones by kinetic protonation (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereoselective synthesis of cis 2,3-disubstituted cycloheptanones by kinetic protonationRui Tamura, Kenichiro Watabe, Akio Kamimura, Kenzi Hori, and Yoshinobu YokomoriCite this: J. Org. Chem. 1992, 57, 18, 4903–4905Publication Date (Print):August 1, 1992Publication History Published online1 May 2002Published inissue 1 August 1992https://doi.org/10.1021/jo00044a027Request reuse permissionsArticle Views158Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A Comprehensive Study of Cyanobacterial Morphological and Ecological Evolutionary Dynamics through Deep Geologic Time (2016)

Cyanobacteria have exerted a profound influence on the progressive oxygenation of Earth. As a complementary approach to examining the geologic record-phylogenomic and trait evolutionary analyses of extant species can lead to new insights. We constructed new phylogenomic trees and analyzed phenotypic trait data using novel phylogenetic comparative methods. We elucidated the dynamics of trait evolution in Cyanobacteria over billion-year timescales, and provide evidence that major geologic events in early Earth's history have shaped-and been shaped by-evolution in Cyanobacteria. We identify a robust core cyanobacterial phylogeny and a smaller set of taxa that exhibit long-branch attraction artifacts. We estimated the age of nodes and reconstruct the ancestral character states of 43 phenotypic characters. We find high levels of phylogenetic signal for nearly all traits, indicating the phylogeny carries substantial predictive power. The earliest cyanobacterial lineages likely lived in freshwater habitats, had small cell diameters, were benthic or sessile, and possibly epilithic/endolithic with a sheath. We jointly analyzed a subset of 25 binary traits to determine whether rates of trait evolution have shifted over time in conjunction with major geologic events. Phylogenetic comparative analysis reveal an overriding signal of decreasing rates of trait evolution through time. Furthermore, the data suggest two major rate shifts in trait evolution associated with bursts of evolutionary innovation. The first rate shift occurs in the aftermath of the Great Oxidation Event and "Snowball Earth" glaciations and is associated with decrease in the evolutionary rates around 1.8-1.6 Ga. This rate shift seems to indicate the end of a major diversification of cyanobacterial phenotypes-particularly related to traits associated with filamentous morphology, heterocysts and motility in freshwater ecosystems. Another burst appears around the time of the Neoproterozoic Oxidation Event in the Neoproterozoic, and is associated with the acquisition of traits involved in planktonic growth in marine habitats. Our results demonstrate how uniting genomic and phenotypic datasets in extant bacterial species can shed light on billion-year old events in Earth's history.
Totally Enclosed Inline Electric Motor Driven Gas Compressors (2000)

A team forms to address the challenge of low cost, low maintenance gas compression that can be quickly ramped up to meet peak demands. The Natural Gas Industry recognizes the importance of efficient, flexible compression equipment for the transmission of gas. In the early 1900s the Gas Industry met its compression objectives with many small reciprocating compressor units. As competition increased, Gas Companies began employing more cost effective larger units 3.7 MW (5,000 bhp) and eventually gas turbines 11+ MW (15,000+ bhp) became the prime mover of choice. While gas fired engine driven compressors are convenient for gas companies; they are becoming increasingly difficult to install. Environmental restrictions have tightened making permitting difficult. The larger gas turbine units seemed a solution because they were the low capital cost prime mover and clean burning. However, gas turbines have not yet achieved the high degree of flexibility and fuel efficiency gas transporters hoped. Flexibility has become an increasingly important issue because of the new “Peaking Power Plants” that are coming online. Gas companies are trying to solve the problem of low cost, low maintenance compression that can be quickly ramped up to meet peak demands. The idea of using electric motors to drive compressors to minimize the environmental, regulatory, and maintenance issues is not new. The idea of installing an electrically powered, highly flexible, efficient, low maintenance compressor unit directly into the pipeline feeding the load, possibly underground where it won’t be seen or heard, is a new and viable way for the gas and electric industries to do business together. This paper examines the application of totally enclosed, variable speed electric motor driven gas compressors to applications requiring completely automated, low maintenance, quick response gas pressure boosters. In this paper we will describe how a natural gas transporter, compressor manufacturer, motor manufacturer, and power company have teamed up to design the world’s first gas compressor that can be installed directly in the pipeline. We will discuss methodologies for installing the proposed compressor, the environmental benefits — no emissions, a small footprint, minimal noise — and the benefit of being able to install compression exactly where it is needed to meet the peaking requirements of today’s new loads.