gebhart/patrepeval huggingface dataset:
RPR 106541, a novel, airways‐selective glucocorticoid: effects against antigen‐induced CD4<sup>+</sup>T lymphocyte accumulation and cytokine gene expression in the Brown Norway rat lung (1997)

1. The effects of a novel 17-thiosteroid, RPR 106541, were investigated in a rat model of allergic airway inflammation. 2. In sensitized Brown Norway rats, challenge with inhaled antigen (ovalbumin) caused an influx of eosinophils and neutrophils into the lung tissue and airway lumen. In the lung tissue there was also an accumulation of CD4+ T lymphocytes and increased expression of mRNA for interleukin-4 (IL-4) and IL-5, but not interferon-gamma (IFN-gamma). These findings are consistent with an eosinophilia orchestrated by activated Th2-type cells. 3. RPR 106541 (10-300 microg kg[-1]), administered by intratracheal instillation into the airways 24 h and 1 h before antigen challenge, dose-dependently inhibited cell influx into the airway lumen. RPR 106541 (100 microg kg[-1]) caused a significant (P<0.01) (98%) inhibition of eosinophil influx and a significant (P<0.01) (100%) inhibition of neutrophil influx. RPR 106541 was approximately 7 times and 4 times more potent than budesonide and fluticasone propionate, respectively. 4. When tested at a single dose (300 microg kg[-1]), RPR 106541 and fluticasone each caused a significant (P<0.01) (100%) inhibition of CD4+ T cell accumulation in lung tissue. Budesonide (300 microg kg[-1]) had no significant effect. RPR 106541 and fluticasone (300 microg kg[-1]), but not budesonide (300 microg kg[-1]), significantly (P<0.05) inhibited the expression within lung tissue of mRNA for IL-4. RPR 106541 (300 microg kg[-1]) also significantly (P<0.05) inhibited expression of mRNA for IL-5. 5. The high topical potency of RPR 106541 in this model, which mimics important aspects of airway inflammation in human allergic asthmatics, suggests that this glucocorticoid may be useful in the treatment of bronchial asthma.
HYDROGEN GENERATION FROM ARTIFICIAL SEA HATER IN A SEMICONDUCTOR SEPTUM ELECTROCHEMICAL PHOTOVOLTAIC CELL (1989)

Visible light of the solar spectrum is directly converted to stored chemical energy of hydrogen from artificial sea water in a novel electrochemical photovoltaic cell. The principal element of the cell, modeled after the photosynthetic thylakoid membrane, is a semiconductor septum made of polycrystalline n-CdSe thin film deposited on nickel foil, which separates two aqueous solutions. Under short-circuit conditions, vigorous hydrogen evolution was seen at the Ni surface and continued as long as the cell was operated. The novel cell, the concept of which was derived from pigmented bilayer lipid membrane studies, is easy to construct, simple to operate, and appears to be a practical approach to the photochemical conversion and storage of solar energy.
Dual crosslinking of carboxylated nitrile butadiene rubber latex employing the thiol‐ene photoreaction (2013)

Abstract At present, the most common used crosslinking process for carboxylated nitrile butadiene rubber (XNBR) latex is an accelerated sulfur curing system with zinc oxide. To avoid allergenic reactions related to residual accelerator levels in dipped XNBR latex articles such as medical gloves, a dual curing process has been developed combining thermal and photochemical crosslinking reactions. The two‐step procedure involves the formation of covalent and ionic bonds to ensure good mechanical properties of the final products. The photochemical thiol‐ene reaction is used to generate covalent crosslinks between the remaining CC double bonds of the butadiene units whereas the carboxylic moieties are conventionally cured with divalent metal oxides (ZnO) under elevated temperature (formation of ionic crosslinks). The photochemical curing step is carried out both in the latex phase using a falling film photoreactor (prevulcanization) as well as in the solid phase by UV irradiation of dried XNBR films (postvulcanization). The mechanical properties and crosslink densities of the cured XNBR films are determined and the influence of selected curing parameters is assessed. The results give evidence that a combined approach of thermal prevulcanization and photochemical postvulcanization makes the production of latex articles (e.g., gloves) with tailored properties and good skin compatibility feasible. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Analysis of Properties of Fiber Composites With Anisotropic Constituents (1979)

Expressions and bounds for the five effective elastic moduli of a unidirectional fiber composite, consisting of transversely isotropic fibers and matrix, are derived on the basis of analogies between isotropic and transversely isotropic elasticity equations. Application of results for determination of the five elastic moduli of graphite fibers is discussed. Effective thermal expansion coefficients are derived on the basis of a general theorem. Effective conductivities, dielectric constants, and magnetic permeabilities are derived by use of certain mathematical analogies.
Sold!: auction methods for multirobot coordination (2002)

The key to utilizing the potential of multirobot systems is cooperation. How can we achieve cooperation in systems composed of failure-prone autonomous robots operating in noisy, dynamic environments? We present a method of dynamic task allocation for groups of such robots. We implemented and tested an auction-based task allocation system which we call MURDOCH, built upon a principled, resource centric, publish/subscribe communication model. A variant of the Contract Net Protocol, MURDOCH produces a distributed approximation to a global optimum of resource usage. We validated MURDOCH in two very different domains: a tightly coupled multirobot physical manipulation task and a loosely coupled multirobot experiment in long-term autonomy. The primary contribution of the paper is to show empirically that distributed negotiation mechanisms such as MURDOCH are viable and effective for coordinating physical multirobot systems.
Retinal tubulin binds macular carotenoids. (1997)

Tubulin is found in abundance in the receptor axon layer of the fovea, where it can serve as a locus for the deposition of the high concentrations of macular carotenoids found there. The binding interaction of carotenoids and tubulin in the Henle's fiber layer could play an important role in the photoprotective effects of the macular carotenoids against the progression of age-related macular degeneration. The association of carotenoids with tubulin, a protein that can form highly ordered linear arrays, may provide an explanation for the dichroic phenomenon of Haidinger's brushes.
Debugging of behavioral VHDL specifications by source level emulation (1995)

We present an approach to accelerate the validation speed of behavioral VHDL system specifications through the use of hardware emulation. The method allows source level debugging of behavioral, algorithmic VHDL in a way similar to source level debugging known from software programming languages. We can set breakpoints in the source code and evaluate the contents of variables by reading the registers of the circuit when a breakpoint is reached.
Hydrogen Peroxide-Induced Deacetylation of Acetyl Resorufin as a Novel Indicator Reaction for Fluorometric Detection of Glucose Using Only Glucose Oxidase. (2001)

Hydrogen peroxide (H2O2)-induced deacetylation of non-fluorescent acetyl resorufin (1) to fluorescent resorufin (2) as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase (GOD) is described. When a 1:1:1 mixture of 1 (in CH3CN), glucose, and GOD (each in pH 7.4 phosphate buffer) was incubated at 25 degrees C under aerobic conditions, the resulting solution turned yellow to fluorescent pink due to 2. The formation of 2 was markedly retarded on incubation under anaerobic conditions. When a mixture of 1 and H2O2 was incubated under aerobic conditions, the formation of 2 was noted as in the case of the enzymatic reaction of 1. These results demonstrated that the observed color change is brought about through deacetylation of 1 to 2 induced by H2O2 generated in GOD-catalyzed oxidation of glucose. With regard to the fluorometric traces of the enzymatic reaction with 1 (0.2 mM), GOD (0.5 mg/ml), and glucose at 25 degrees C, fluorescence intensity exhibited a linear relationship against glucose concentration between 0.2 and 2.0 mm, with a correlation coefficient of 0.997. Neither ascorbic acid, uric acid, nor bilirubin significantly interfered with the transformation of 1 to 2 through GOD-catalyzed oxidation of glucose.
Renal Drug Delivery With Low-Molecular-Weight Proteins: The Effect of Charge Modifications on the Body Distribution of Drug-Lysozyme Conjugates (1999)

Low-molecular-weight proteins (LMWPs) can be used as drug carriers for renal drug delivery. The covalent attachment of drug molecules is commonly achieved via reaction to the primary amino groups of the protein. However, these positively charged groups are also important for the renal reabsorption of proteins. In this study, we investigated the effect of the modification of primary amino groups on the pharmacokinetics of drug-LMWP conjugates, using the organic anion fluorescein isothiocyanate (FITC) as a model drug and the LMWP lysozyme (LZM) as a drug carrier. We prepared a series of conjugates with different amounts of primary amino groups: a more negatively charged conjugate was prepared by derivatizing the primary amino groups of LZM with succinic anhydride, while a more positively charged conjugate was prepared by derivatizing the carboxylic acid groups with ethylendiamine. The final conjugates had an average amount of primary amino groups of 0.2 (FITC-Suc-LZM), 6.5 (FITC-LZM), or 8.5 (FITC-cat-LZM). We studied the renal handling of the conjugates in freely moving Wistar rats. After i.v. injection of 33 mg/kg conjugate, the FITCLZM and the FITC-Suc-LZM conjugates were incompletely reabsorbed in the kidney, resulting in the excretion of the intact conjugate into the urine (29 4% versus 45 4% of the injected dose, p &lt; 0.05). In contrast, the FITC-cat-LZM conjugate was not excreted into the urine (0.3 0.1% of the injected dose, p &lt; 0.001 distributed selectively to the kidney, and significant amounts of this versus FITC-LZM). An organ distribution study with 125I radiolabeled cat-LZM showed that the cationized LMWP accumulated in the kidney (12 0 . 3% of the dose). However, cat-LZM no longer LMWP were found in the liver and spleen (53 1% and 5 2%, respectively). We conclude that charge modifications influence the biodistribution of drug-LZM conjugate. First, reducing the amount of primary amino groups results in decreased tubular reabsorption and increased excretion of the conjugate into the urine. Finally, increasing the amount of primary amino groups results not only in improved renal reabsorption, but also in an increased extrarenal distribution of the conjugate and therefore in a partial loss of renal selectivity.
Analysis of Complex Decisionmaking Processes (1978)

The analysis of corporate decisionmaking processes related to major system developments is unusually difficult because of the number of decisionmakers involved in the process and the long development cycle. A method for analyzing such decision processes is developed and illustrated through its application to the analysis of the commercial jet engine development process. The method uses interaction matrices as the key tool for structuring the problem, recording data, and analyzing the data to establish the rank order of the major factors affecting development decisions. In the example, the use of interaction matrices permitted analysts to collect and analyze approximately 50 factors that influenced decisions during the four phases of the development cycle, and to determine the key influencers of decisions at each development phase. The results of this study indicate that the cost of new technology installed on an aircraft is the prime concern of the engine manufacturer. Also, the manufacturer is strongly influenced by his customers during the initial stage of the development; however, once the development program has been accepted, the technical manager has the strongest influence on the development of the new product. The application of the interaction matrix is shown to be a viable method to structure and analyze the complex process of decisionmaking in a high technology industry such as the commercial aircraft engine industry.
Interactive, Constraint-based Layout of Engineering Diagrams (2024)

Many engineering disciplines require visualisation of networks. Constrained graph layout is a powerful new approach to network layout that allows the user to impose a wide variety application-specific placement constraints—such as downwards pointing directed edges, alignment of nodes, cluster containment and non-overlapping nodes and clusters—on the layout. We have recently developed an efficient algorithm for topology-preserving constrained graph layout. This underpins two dynamic graph layout applications we have developed: a network diagram authoring tool, Dunnart, and a network diagram browser. In this paper we provide an overview of topology-preserving constrained graph layout and illustrate how Dunnart and the network diagram browser can be applied to engineering diagram authoring and visualisation.
Advanced molecular and cytogenetic technologies in birth defect diagnosis and prevention. (2005)

Fluorescence in situ hybridization (FISH) has become an important diagnostic tool as an adjunct to classical cytogenetics. FISH utilizes DNA probes comprised of specific nucleic acid sequences tagged with fluorescent molecules to identify the number and location of specific DNA sequences in human cells. These probes can be used to determine various numerical and structural chromosomal aberrations, in many cases, gene dosage and/or structure alterations. Chromosomal abnormalities are responsible for a considerable number of birth defects, and more than 50% of spontaneous abortions. These numbers have been significantly higher since the advent of FISH technology that allows the detection of submicroscopic chromosome alterations. The clinic application of FISH technology in postnatal, prenatal, and preimplantation diagnoses has been playing an important role in the diagnosis and prevention of birth defects. As new technologies evolve, more and more new FISH techniques-such as subtelomeric FISH, multicolor FISH (M-FISH), comparative genomic hybridization (CGH), and microarray-are used in clinical diagnoses, the role of FISH technology in both research and clinical aspects of birth defects will surely continue to expand.
Microdistribution of Oxygen in Silicon (1980)

The microdistribution of oxygen in Czochralski‐grown, P‐type silicon crystals (75 mm in diameter and with a resistivity of 10 Ω‐cm) was determined by using the SEM in the EBIC mode in conjunction with spreading resistance measurements. The crystals were heat‐treated at 450°C to activate oxygen donors. When the conductivity remained p‐type, (the oxygen donor concentration did not exceed the dopant concentration), bands of contrast were observed in the EBIC image which were found to correspond to maxima in resistivity, i.e., to dopant compensation maxima and hence, to maxima in oxygen concentration. When at the oxygen concentration maxima the oxygen donor concentration exceeded (locally) the p‐type dopant concentration, an inversion of the conductivity occurred. It resulted in the formation of p‐n junctions in a striated configuration and the local inversion of the EBIC image contrast. A chemical etching procedure was developed, in conjunction with phase contrast microscopy, to delineate the n‐type oxygen striations. By heat‐treating silicon at 1000°C prior to the activation of oxygen donors, some silicon‐oxygen micro‐precipitates were observed in the EBIC image within the striated oxygen concentration maxima.
Gene conversion contributes to Ig light chain diversity in cattle (1996)

In humans and mice, extensive gene rearrangement is the major mechanism of diversification of the primary Ig repertoire. This study shows that cattle depart from this pattern because rearrangement in the light chain locus is sharply limited. Furthermore, in cattle, gene conversion contributes to the diversification of the primary light chain repertoire. Sequencing of germ-line and expressed Vlambda genes revealed three important features. First, the germ line contained a number of Vlambda pseudogenes. In fact, 14 (70%) of the 20 germ-line genes identified and sequenced were pseudogenes, because they had one or more of the following defects: lack of recombination signal sequences at the 3' end, stop codons within the reading frame or truncations, and/or insertions or deletions that resulted in loss of reading frame. Second, Vlambda cDNA from ileal Peyer's patch B cells demonstrated that the light chain repertoire arises from only a small number of V(J) rearrangements. Even though two J genes were identified in the germ line, all of the expressed Vlambda genes examined contained the same J segment, indicating that only a single J gene participates in rearrangement at the lambda locus. Third, a significant number of departures from the germ-line sequences of rearranged Vlambda can be traced to donor sequences of one or more Vlambda pseudogenes. We conclude that a limited number of rearrangements and gene conversion play a role in contributing to the diversification of the primary lambda repertoire. Furthermore, while clear indications of a role for somatic mutation in lambda diversification was seen, V gene rearrangement was not a major factor.
Investigations of the effects of blade type, dicing tape, blade preparation and process parameters on 55nm node low-k wafer (2010)

This paper presents an investigation of the effects of blade type, dicing tape, blade preparation and the key process parameters optimization on improving topside ILD peeling (thicker scribe structures) and chipping for 55nm low-k wafer. An appropriate dicing blade selection, blade preparation / conditioning methodology and dicing tape selection plays an important role in developing a robust saw process. As such, experimental studies were conducted under varying Z1 spindle rotation, Z1 cut depth into Si as well as the blade type property variation as the input factors, in order to improve the ILD peeling and die chipping. The settings of machining parameters and blade types were determined by using the design of experiment (DOE) techniques and the critical process parameters and materials were analyzed statistically by using the analysis of variance (ANOVA). Dicing tape property variations (PO-base or PVC-base) as well as the blade preparation methodology posed some influences on the overall dicing quality, such as die backside chipping, die removal performance, ILD peeling and die topside chipping. SEM imaging and optical visual inspection were conducted to validate the impacts of the ILD peeling / chipping on post-processed low-k wafers. A thorough quantification and categorization of ILD peeling and chipping on heavy metallization at the saw scribe structures were described. As part of the recommendation for future works, a different approach in dicing technology, namely laser grooving was proposed to eliminate ILD peeling and chipping. In conclusion, the optimized dicing recipe for 55nm node low-k wafer suggested by the DOE model are: (1) a thinner PO-base dicing tape, (2) a dicing blade with higher diamond concentration and finer grit size, (3) blade preparation / conditioning done with SiC board and (4) processing at lower spindle rotation and deeper cut depth are much preferred. The overall dicing responses and cutting quality has improved and is better compared to current production recipe.
MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis (2001)

The first step of methylthioribose recycling is phosphorylation by MTR kinase, coded by the mtnK (formerly ykrT) gene. Analysis of the neighbourhood of mtnK demonstrates that genes located in its immediate vicinity (now named mtnUVWXYZ, formerly ykrUVWXYZ) are also required for methylthioribose recycling.
Sequence specificity of furin, a proprotein-processing endoprotease, for the hemagglutinin of a virulent avian influenza virus (1994)

The virulence of avian influenza viruses correlates with the sensitivity of their hemagglutinin (HA) to cellular proteases. Furin, a proprotein-processing subtilisin-related endoprotease, is a leading candidate for the enzyme that cleaves the HA of virulent avian viruses. We therefore compared the specificity of furin with those of proteases in a variety of cultured cells and in a rat Golgi fraction, using the HA cleavage mutants of a virulent avian influenza virus, A/Turkey/Ireland/1378/85 (H5N8). The results indicated similar sequence specificities among the endoproteases when purified furin was used. In experiments with the vaccinia virus expression system, overexpressed furin cleaved mutant HAs that were not recognized by the endogenous proteases, resulting in an apparent broader specificity of furin. These findings authenticate the proposed role of furin as an HA-activating protease in vivo and caution against the use of expression vectors to study protease sequence specificity.
Caspase-11 Mediates Oligodendrocyte Cell Death and Pathogenesis of Autoimmune-Mediated Demyelination (2001)

Multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE), are inflammatory diseases of the central nervous system (CNS) characterized by localized areas of demyelination. The mechanisms underlying oligodendrocyte (OLG) injury in MS and EAE remain unknown. Here we show that caspase-11 plays crucial roles in OLG death and pathogenesis in EAE. Caspase-11 and activated caspase-3 were both expressed in OLGs in spinal cord EAE lesions. OLGs from caspase-11-deficient mice were highly resistant to the cell death induced by cytotoxic cytokines. EAE susceptibility and cytokine concentrations in the CNS were significantly reduced in caspase-11-deficient mice. Our findings suggest that OLG death is mediated by a pathway that involves caspases-11 and -3 and leads to the demyelination observed in EAE.
Synthesis of <i>p</i>-(Di-<i>tert</i>-butyl[<sup>18</sup>F]fluorosilyl)benzaldehyde ([<sup>18</sup>F]SiFA-A) with High Specific Activity by Isotopic Exchange: A Convenient Labeling Synthon for the <sup>18</sup>F-Labeling of N-amino-oxy Derivatized Peptides (2007)

The syntheses of different (18)F-labeled peptides using the highly effective labeling synthon p-(di- tert-butylfluorosilyl) benzaldehyde ([ (18)F]SiFA-A) for the development of (18)F-radiopharmaceuticals for oncological positron emission tomography (PET) is reported. The novel and mild labeling technique for the radiosynthesis of [ (18)F]SiFA-A, based on an unexpectedly efficient isotopic (19)F- (18)F exchange, yielded the (18)F-synthon [ (18)F]SiFA-A in almost quantitative yields in high specific activities between 225 and 680 GBq/micromol (6081-18 378 Ci/mmol) without applying HPLC purification. The [ (18)F]SiFA-A was finally used to label the N-terminal amino-oxy (N-AO) derivatized peptides AO-Tyr (3)-octreotate (AO-TATE), cyclo(fK(AO-N)RGD and N-AO-PEG 2-[D-Tyr-Gln-Trp-Ala-Val-betaAla-His-Thi-Nle-NH 2] (AO-BZH3, a bombesin derivative) in high radiochemical yields. Density functional theory (DFT) calculations confirmed high efficiency of the isotopic exchange, which is predicted to proceed via a pentacoordinate siliconate intermediate dissociating immediately to form the radiolabeled [ (18)F]SiFA-A.