gebhart/patrepeval huggingface dataset:
Human Cytomegalovirus Glycoprotein B Is Required for Virus Entry and Cell-to-Cell Spread but Not for Virion Attachment, Assembly, or Egress (2009)

Glycoprotein B (gB) homologs are conserved throughout the family Herpesviridae and appear to serve essential, universal functions, as well as specific functions unique to a particular herpesvirus. Genetic analysis is a powerful tool to analyze protein function, and while it has been possible to generate virus mutants, complementation of essential virus knockouts has been problematic. Human cytomegalovirus (HCMV) gB (UL55) plays an essential role in the replication cycle of the virus. To define the function(s) of gB in HCMV infection, the BAC system was used to generate a recombinant virus in which the UL55 gene was replaced with galK (pAD/CreDeltaUL55). UL55 deletions in the viral genome have been made before, demonstrating that UL55 is an essential gene. However, without being able to successfully complement the genetic defect, a phenotypic analysis of the mutant virus was impossible. We generated fibroblasts expressing HCMV gB that complement pAD/CreDeltaUL55 and produce infectious virions lacking the UL55 gene but containing wild-type gB on the virion surface (DeltaUL55-gB HCMV). This is the first successful complementation of an HCMV mutant with a glycoprotein deleted. To characterize DeltaUL55 infection in the absence of gB, noncomplementing cells were infected with DeltaUL55-gB virus. All stages of gene expression were detected, and significant amounts of DNase-resistant viral DNA genomes, representing whole intact virions, were released into the infected cell supernatant. Gradient purification of these virions revealed they lacked gB but contained other viral structural proteins. The gB-null virions were able to attach to the cell surface similarly to wild-type gB-containing virions but were defective in virus entry and cell-to-cell spread. Glycoprotein B-null virions do, however, contain infectious DNA, as IE gene expression can be detected in fibroblasts following treatment of attached gB-null virions with a membrane fusion agent, polyethylene glycol. Taken together, our results indicate that gB is required for virus entry and cell-to-cell spread of the virus. However, HCMV gB is not absolutely required for virus attachment or assembly and egress from infected cells.
CD155/PVR plays a key role in cell motility during tumor cell invasion and migration (2004)

These results implicate a role for CD155 in mediating tumor cell invasion and migration and suggest that CD155 may contribute to tumorigenesis.
Preparation of spherical tungsten and titanium powders by RF induction plasma processing (2014)

Abstract Spherical tungsten and titanium powders were prepared by radio frequency inductively coupled plasma torch with irregular shaped tungsten and titanium hydride (TiH 2 ) powders, respectively. The effect of the feed rate on spheroidization efficiency was investigated. The phase composition, oxygen content, morphology, and particle size distribution of the powders before and after spheroidization were characterized by X‐ray diffraction (XRD), nitrogen/oxygen analyzer, scanning electron microscopy (SEM), and laser micron sizer (LMS). The results show that both kinds of plasma‐processed powders have good dispersity, smooth surface, and single phase. A maximum of 100 % of spheroidization efficiency can be achieved at a lower feed rate. The spherical titanium powder obtained by the plasma treatment consists of particles with mean diameter of 33.34 μm, while the mean diameter of original TiH 2 powder is 136.56 μm. The apparent density and flowability of the spherical tungsten powder are 6.3 g·cm −3 and 0.16 s·g −1 , respectively. The apparent density and flowability of the spherical titanium powder are 2.8 g·cm −3 and 0.52 s·g −1 , respectively. With the increase of the feed rate, the spheroidization efficiency of raw powders drops gradually.
Androgen deprivation causes truncation of the <scp>C</scp>‐terminal region of androgen receptor in human prostate cancer <scp>LNCaP</scp> cells (2012)

The androgen receptor (AR) acts as a ligand-dependent transcription factor, whereas mutant AR lacking the C-terminal ligand-binding domain functions in a ligand-independent manner. In the present study we report that the C-terminal truncated AR, which we named AR-NH1 (the N-terminal fragment of AR cleaved in the neighborhood of helix 1 of the ligand-binding domain), is produced in LNCaP prostatic carcinoma cells. The AR-NH1 of ~90 kDa was observed in an androgen-independent LNCaP subline and was further accumulated by the proteasome inhibitor MG132. MG132 treatment caused the accumulation of AR-NH1 even in parent LNCaP cells. AR-NH1 was produced in the absence of ligand or in the presence of the AR antagonist bicalutamide, whereas AR agonists suppressed its production. AR-NH1 was detected with different AR antibodies recognizing amino acid residues 1-20 and 300-316 and was also generated from exogenous AR. Both siRNA-mediated AR knockdown and treatment with a serine protease inhibitor (4-(2-aminoethyl)-benzenesulfonyl fluoride) reduced AR-NH1 levels. According to the predicted cleavage site (between amino acid residues 660-685) and its nuclear localization, it is assumed that AR-NH1 functions as a constitutively active transcription factor. These data suggest that AR-NH1 is produced under hormone therapy and contributes to the development of castration-resistant prostate cancer due to its ligand-independent transcriptional activity.
[Traumatic optic neuropathy: importance and result of surgical treatment]. (2002)

The surgical treatment is important for elevating curative effect of TON.
Coupling and decoupling of electromagnetic waves in parallel 2D photonic crystal waveguides (2002)

The behavior of two nearby straight photonic crystal waveguides is analyzed. It is shown that the two guides, considered as a single system, may realize a very efficient wavelength selective directional coupler, that may be used as a channel interleaver in a WDM communications system. In addition, we also show that, by properly designing the geometry of the dielectric region between the guide cores, waveguide decoupling can be obtained. Necessary conditions for this feature to be obtained are analytically derived, and an electromagnetic explanation of the decoupling process is given.
Hyperspectral Confocal Fluorescence Microscope: A New Look into the Cell (2010)

Confocal microscopy is widely used in cell biology. Like other filter-based systems, traditional confocal microscopes are limited by the spectral bands established by each optical filter. As a result, emission spectra from labels and/or autofluorescence can be overlapped leading to spectral crosstalk and inability to quantify the amount of signal originating from each individual fluorescent species. The need for accurate quantification of in vivo cellular processes and in-depth knowledge of organelle development and microstructure led Monsanto to search for non-commercial microscopes that could achieve those goals. Through a cooperative research and development agreement (CRADA) established between Monsanto and Sandia Corporation in August 2006, we built a new 3D-hyperspectral confocal fluorescence imaging system, specifically designed to meet the analytical requirements of plant specimens.
Positron emission tomography/computed tomography and radioimmunotherapy of prostate cancer (2009)

PET/CT in prostate cancer has proven to play a significant role, in particular for detection of prostate cancer recurrence and bone metastases. RIT of metastatic prostate cancer warrants further investigations.
Design and fabrication of a surface-illuminated-type semiconductor optical modulator with electron-depleting absorption control (1998)

Design and test fabrication were carried out for a surface-illuminated-type semiconductor optical modulator with electron-depleting absorption control. In a theoretical design example with optimum conditions, it is expected that the extinction ratio is about 10 dB with a voltage change of –5 V, the insertion loss is less than 1 dB, and the operating speed is several hundred megahertz to several gigahertz. On the other hand, the extinction ratio of a surface-illuminated-type modulator fabricated for a preliminary experiment was about 10 dB with a voltage change of –7.5 V. An optical transmission-type modulator operated at a low driving voltage is realized. © 1998 Scripta Technica, Electron Comm Jpn Pt 2, 81(11): 42–53, 1998
A single system image server cluster using duplicated MAC and IP addresses (2002)

Clusters of PCs are increasingly being used to build high-capacity Web sites. We develop and evaluate a method of building a single system image (SSI) cluster that does not require a central dispatcher. In our clone cluster method, which builds on the existing ONE-IP method, a group of mirrored server machines with identical MAC and IP addresses are configured on a shared-medium LAN. Each machine filters incoming IP packets based on a hash of IP source addresses. Unlike ONE-IP, our method does not require router configuration changes. We build and evaluate a Linux-based, two-PC cluster that uses an odd/even filter. Simulation results show that the effect of server selection method on mean response time is less significant for heavy-tailed interarrival times than for exponential interarrival times. Simulation results show that response time performance from a Clone Cluster can be very close to that of a centrally dispatched least-loaded cluster.
Intestinal Obstruction Caused by Spontaneous Transomental Internal Hernia (2007)

A transomental hernia is a rare type of internal hernia. Only one case of this type of hernia has been reported in Korea whereas several cases have been described in other countries. This is the second case of a mechanical obstruction caused by a transomental internal hernia in Korea. We report this case with a brief review of the relevant literature. (J Korean Surg Soc 2007;73:511-513)
Deep submicrometer double-gate fully-depleted SOI PMOS devices: a concise short-channel effect threshold voltage model using a quasi-2D approach (1996)

This paper reports a concise short-channel effect threshold voltage model using a quasi-2D approach for deep submicrometer double-gate fully-depleted SOI PMOS devices. By considering the hole density at the front and the back channels simultaneously, the analytical threshold voltage model provides an accurate prediction of the short-channel threshold voltage behavior of the deep submicrometer double-gate fully-depleted SOI PMOS devices as verified by 2D simulation results. The analytical short-channel effect threshold voltage model can also be useful for SOI NMOS devices.
Inhibition of Host Transcription by Vesicular Stomatitis Virus Involves a Novel Mechanism That Is Independent of Phosphorylation of TATA-Binding Protein (TBP) or Association of TBP with TBP-Associated Factor Subunits (2001)

ABSTRACT The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor in vivo of transcription by all three host RNA polymerases (RNAP). In the case of host RNA polymerase II (RNAPII), the inhibition is due to lack of activity of the TATA-binding protein (TBP), which is a subunit of the basal transcription factor TFIID. Despite the potency of M protein-induced inhibition in vivo, experiments presented here show that M protein cannot directly inactivate TFIID in vitro. Addition of M protein to nuclear extracts from uninfected cells did not inhibit transcription activity, indicating that the inhibition is indirect and is mediated through host factors. The host factors that are known to regulate TBP activity include phosphorylation by host kinases and association with different TBP-associated factor (TAF) subunits. However, TBP in VSV-infected cells was found to be assembled normally with its TAF subunits, as shown by ion exchange high-pressure liquid chromatography and sedimentation velocity analysis. A normal pattern of phosphorylation of TBP in VSV-infected cells was also observed by pH gradient gel electrophoresis. Collectively, these data indicate that M protein inactivates TBP activity in RNAPII-dependent transcription by a novel mechanism, since the known mechanisms for regulating TBP activity cannot account for the inhibition.
The Neurobiology Of Alzheimer’S Disease (1996)

Significant developments in our understanding of the pathophysiology of Alzheimer's disease have been obtained in the recent years. Diagnostic criteria, based on clinical data, have been proposed and have been validated by clinico-pathological correlations. Some neuroimaging techniques and laboratory tests (e.g. dosage in the cerebrospinal fluid) are promising diagnostic avenues. Genetic mutations associated with familial cases of the disease have been identified and the involved genes localized on chromosome 1, 14 or 21. The apolipoprotein E genotype has been discovered to affect the risk of developing the disease, i.e. homozygotes for the apolipoprotein E4 allele are much more prone to develop Alzheimer's disease The definitive diagnosis of the disease still relies on the demonstration of characteristic neuropathological lesions, i.e. neurofibrillary tangles and senile plaques, whose numbers are correlated with the severity of the dementia. Other lesions include neuronal and synaptic loss, amyloid angiopathy, and severe decrease in the level of cortical acetylcholine. Neurofibrillary tangles have been found to be composed of the microtubule-associated protein tau, in highly phosphorylated state. The accumulation of these phosphorylated tau proteins is thought to be associated to disturbances of intracellular transport of molecules and organelles in affected neurones, leading to cell dysfunction and death. An inbalance in the activities of selected protein kinases and phosphatases is also thought to generate these highly phosphorylated tau species. The major component of senile plaques is the A4/beta amyloid peptide, generated by proteolysis of the amyloid peptide precursor, a transmembrane protein. When aggregated into amyloid fibrils, the A4/beta amyloid peptide is thought to be neurotoxic. An abnormal metabolism of the amyloid peptide precursor is often considered as a central physiopathological mechanism of the disease. Although many pharmacological treatments of the disease have been investigated, they have not yet led to sustained and major clinical improvements.
Development of an Orthotopic Model of Invasive Pancreatic Cancer in an Immunocompetent Murine Host (2010)

We have developed a rapid, predictable orthotopic model of pancreatic adenocarcinoma in immunocompetent mice that mimics human pancreatic cancer with regard to genetic mutations, histologic appearance, and pattern of disease progression. This model highlights both the complexity and relevance of the immune response to invasive pancreatic cancer and may be useful for the preclinical evaluation of new therapeutic agents.
Near-Field Analysis of Surface Waves Launched at Nanoslit Apertures (2007)

With the aim of analyzing the properties of the waves that are scattered by nanoslits on metallic surfaces, we provide a direct observation of the near-field in a slit-doublet experiment at optical wavelengths. We show that two distinct waves are involved: a surface plasmon polariton and another wave with a free-space character. From the recorded data, we have extracted the amplitudes and phases of these waves, their damping characteristic lengths and their relative weights as a function of the separation distance from the slit. The analysis is fully supported by a quantitative agreement with vector-theory computational results.
Composite media (dynamic tilted media) for magnetic recording (2005)

We designed and fabricated a composite magnetic recording medium with exchange decoupled magnetic grains that consist of two vertically exchange-coupled magnetic regions (one is magnetically soft and one is magnetically hard) as an approach to alleviate the writing field limitation of perpendicular magnetic recording heads. A nonmagnetic layer with different thickness was put between the hard and soft layer to tune the exchange coupling. With proper coupling, significant drop of the coercivity field was observed for this composite medium while still maintaining good thermal stability. Better recording performance was obtained for such medium compared to perpendicular and longitudinal medium. The results have proved the possibility of fabricating a writable recording medium having an ultrahigh magnetic anisotropy constant (Ku) value.
Antitumor Effect of MAb EMD 55900 Depends on EGF-R Expression and Histopathology (2002)

The proliferative stimulus of the epidermal growth factor (EGF) in human epithelial cells is mediated by its binding to the external domain of the EGF receptor (EGF-R). The purpose of this study was to investigate whether growth arrest of tumors treated with anti-EGF-R MAb (EMD 55900) was dependent on EGF-R expression and distinct histopathologic criteria of those neoplasms. Nine different adenocarcinomas, squamous cell carcinomas and two neoplastic epithelial cell lines (A431 and Detroit 562), which were characterized by high EGF-R expression, were xenotransplanted onto NMRI-nu/nu mice and treated with an anti-EGF-R antibody (EMD 55900). Results revealed that EGF-R expression and distinct histopathologic growth patterns play an important role for the therapeutic effect of the EGF-R antibody treatment. Tumors with high epithelial cellularity and little connective tissue responded to EMD 55900 treatment to a greater degree of growth reduction than tumors with lower cellularity. These results will be helpful for evaluation of patients who would benefit from tumor therapy with anti-EGF-R antibody.
Red and Processed Meat Consumption and Risk of Incident Coronary Heart Disease, Stroke, and Diabetes Mellitus (2010)

Consumption of processed meats, but not red meats, is associated with higher incidence of CHD and diabetes mellitus. These results highlight the need for better understanding of potential mechanisms of effects and for particular focus on processed meats for dietary and policy recommendations.
Computed Tomography of Hepatic Morphologic Changes in Cirrhosis of the Liver (1986)

Computed tomography of 75 biopsy proven cirrhotic patients was analyzed volumetrically and compared with CT on 50 normal subjects in an attempt to quantitate hepatic morphologic changes specific for cirrhosis. Our data show that the mean percentage of the total liver volume occupied by the right hepatic lobe decreased by 15.2% (p less than 0.0001) and the mean percentage of the total liver volume occupied by the medial segment of the left lobe decreased in volume by 10.9% (p less than 0.09) when compared with normals. Concomitantly, the mean percentage of the total liver volume occupied by the caudate lobe increased by 192% (p less than 0.0001) and the mean percentage of the total liver volume occupied by the lateral segment of the left lobe increased by 55.6% (p less than 0.0001). This increase in the volume of the lateral segment of the left lobe and decrease in the volume of the medial segment of the left lobe have not been described previously. The cirrhotic patients were divided according to etiology of their disease: alcoholic or nonalcoholic. No statistically significant difference was found in the morphologic changes based on etiology when the data were examined using analysis of variants.
Evaluation of CFR and FFR parameters by CFD modeling of the flow in a stenosed coronary artery (2003)

Medical specialists estimate stenosis severity using either simple geometrical parameters, such as percent diameter stenosis, or hemodynamically based parameters such as the fractional flow reserve (FFR) or coronary flow reserve (CFR). The present study is carried to establish a relationship between actual hemodynamic conditions and the above mentioned parameters of stenosis severity. We use a numerical model of the blood flow in a vessel with a blunt stenosis and autoregulated vascular bed to simulate a stenosed blood vessel. A key point to realistic simulations is to model properly the arterial autoregulation. The present numerical model is based on the comparison of the flow in healthy and stenotic vessels. The calculated values of CFR and FFR are in the physiological range.
Identification of three novel mutations in the USH1C gene and detection of thirty-one polymorphisms used for haplotype analysis (2000)

Usher syndrome (USH) is a clinically and genetically heterogeneous autosomal recessive disorder in which sensorineural hearing loss is associated with retinitis pigmentosa. Usher syndrome type 1, the most severe form, is characterized by profound congenital deafness, vestibular dysfunction, and prepubertal onset of retinitis pigmentosa. Six different USH1 genes have so far been mapped, of which two have already been identified. MYO7A, encoding the unconventional myosin VIIA, underlies USH1B. Recently, the USH1C gene was shown to encode harmonin, a PDZ domain-containing protein. A previous screening of 18 unrelated USH1 patients, without a detected MYO7A mutation, for the three USH1C mutations described to date had demonstrated the presence of the 238-239insC mutation in the heterozygous state in four of them. A complete USH1C mutation screening in these four carriers of the 238-239insC mutation resulted in the detection of the second mutation in all the individuals, and the identification of three novel mutations, namely two splice site mutations (IVS1+1G>T and IVS5+1G>A) and a nonsense mutation (R31X). Thirty-one polymorphisms were detected in the USH1C gene. We observed that the E519D substitution is non-pathogenic, which is of particular interest for molecular diagnosis. Our analysis indicated that all the carriers of the 238-239insC mutation share a common haplotype. A different common haplotype was found in the two IVS1+1G>T carriers. Future studies of additional carriers and non-carriers should document the here proposed founder effect of these two mutations.
Transfusion‐related acute lung injury (TRALI): a serious adverse event of blood transfusion (2005)

By virtue of its morbidity and mortality, TRALI has become one of the most serious current complications of transfusion. To prevent further antibody-mediated cases, the evaluation of TRALI should include leucocyte antibody testing of implicated donors. However, further studies are necessary for the prevention of this serious transfusion complication.
Heparin protects basic and acidic FGF from inactivation (1986)

The ability of heparin or that of hexuronyl hexosaminoglycan sulfate (HHS-4) to protect basic or acidic fibroblast growth factor (FGF) from acid or heat inactivation has been analyzed. Both freshly prepared basic and acidic FGF stimulate the growth of baby hamster kidney (BHK-21) cells exposed to medium supplemented with transferrin and insulin. Freshly prepared basic FGF was 10 fold more potent than acidic FGF. The addition of heparin to the medium decreased the potency of basic FGF while it potentiated that of acidic FGF. Upon storage of FGF at -80 degrees C, a decline in potency of both basic and acidic FGF was observed. Heparin, when added to the medium, potentiated their activities, which became similar to that of freshly prepared basic FGF. In order to test whether heparin could protect basic or acidic FGF from inactivation, both mitogens were exposed to acid conditions (1% trifluoroacetic acid, pH 1.08, 2 h) or heat (65 degrees C, 5 min) which inactivate basic or acidic FGF. When exposed to such treatment in the presence of heparin or HHS-4, basic and acidic FGF retained their potency. The effect of heparin and HHS-4 on the bioactivity of basic and acidic FGF is truly of a protective nature, since they had no effect when added after inactivation of the mitogens. Potentiation of the bioactivity of the protected mitogens or of the inactivated one could only be observed when cells were exposed to high heparin or HHS-4 concentrations. This indicates that heparin and HHS-4, in addition to protecting FGF from inactivation, also acts at another locus, as yet unidentified.