gebhart/patrepeval huggingface dataset:
High-Temperature, Borate-Crosslinked Fracturing Fluids: A Comparison of Delay Methodology (1993)

ABSTRACT Borate crosslinked guar fracturing fluids have recently been re-engineered to allow use at higher temperatures in both fresh water and seawater. A key part of this work involves control of the crosslink delay time of the fluid. This report presents an overview of the technology available to control delay time with an emphasis on the types of processes used in current commercial systems. Control of delay time requires control of either the pH or the availability of borate ions or both. To date, control of pH can be effective in freshwater systems. Control of borate is effective in both fresh water and seawater. This may be accomplished by using low-solubility borate species or complexing the borate with a variety of organic species.
Enhanced Acoustic Separation of Oil-Water Emulsion in Resonant Cavities (2008)

This paper explores the use of acoustics for the recovery of oil in oil-water emulsions. A series of experimental studies were conducted using acoustic standing waves in resonant cavities as means of trapping oil droplets and enhancing oil separation. Several cavity configurations were explored and the frequency range used was between 1 MHz-2 MHz. Oil-water emulsions studied were made using mineral oil and motor oil.
In Vivo Noninvasive Imaging for Gene Therapy (2003)

Gene therapy is reaching a stage where some clinical benefits have been demonstrated on patients involved in phase I/II clinical trials. However, in many cases, the clinical benefit is hardly measurable and progress in the improvement of gene therapy formulations is hampered by the lack of objective clinical endpoints to measure transgene delivery and to quantitate transgene expression. However, these endpoints rely almost exclusively on the analysis of biopsies by molecular and histopathological methods. These methods provide only a limited picture of the situation. Therefore, there is a need for a technology that would allow precise, spacio-temporal measurement of gene expression on a whole body scale upon administration of the gene delivery vector. In the field of gene therapy, a considerable effort is being invested in the development of noninvasive imaging of gene expression and this review presents the various strategies currently being developed.
Permeation equations developed for prediction of membrane performance in pervaporation, vapor permeation and reverse osmosis based on the solution-diffusion model. (1991)

General permeation equations based on the solution-diffusion model were proposed for pervaporation (PV), vapor permeation (VP) and reverse osmosis (RO) on two different assumptions about the pressure gradient inside a membrane: a flat gradient (case 1) and a linear gradient (case 2). With these equations the permeation properties in PV, RO and VP can be estimated once the transport parameter of a membrane is known.The effect of upstream pressure on selectivity and flux in RO and PV was estimated by sample calculations for water- and ethanol-selective membranes in ethanol–water system. Flux and selectivity in RO is smaller and, reaching that in PV at infinite pressure. This ultimate value is different in cases 1 and 2, and in the latter the molar volume ratio of the permeants becomes important. The effect of downstream pressure in PV was also estimated and compared with the case of vacuum-enhanced membrane distillation (MD) with a porous membrane. With increasing pressure the separation factor approaches that of vapor–liquid equilibrium in both PV and MD. With decreasing pressure that in MD is governed by the ratio of diffusion coefficients inside the membrane. Since the Knudsen diffusion coefficient of water is larger than that of ethanol, the separation factor decreases in ethanol–water separation with decreasing downstream pressure. This was verified by experiment, using PTFE membranes.
Comparison of methylation level of genomes among different animal species and various tissues (2007)

Abstract The methylation levels of genomes were compared in swine, cattle, sheep, rat, chicken and duck, using the methylation-sensitive amplification polymorphism technique (MSAP). The results showed that the methylation levels in genomes of the species investigated were mostly about 40–50% (except cattle); the methylation level varied in different species; the methylation pattern in various tissues of each species was specific; for the same species, the methylation level of the tissue genome was mostly higher than that of the blood genome; the difference of methylation level between birds and mammals was not significant, however mammals appeared to have a lower hemi-methylation frequency and higher full methylation frequency than birds.
Cytomegalovirus infection in pregnancy (2017)

Cytomegalovirus (CMV) is a DNA herpesvirus that is common worldwide. The two known main sources of primary CMV infection during pregnancy are through sexual activity and contact with young children. Primary infection occurs in approximately 1 to 4% of pregnancies, and is mostly asymptomatic in immunocompetent adults. However, primary infection may manifest as a mild mononucleosis or flu-like syndrome with persistent fever and fatigue. CMV can be transmitted from mother-to-child in utero, intrapartum, or during breastfeeding. Intrauterine transmission can lead to congenital CMV infection, a leading cause of permanent hearing and vision loss and neurological disability among children. Congenital CMV transmission rates are as high as 50% in women who acquire primary CMV infection during pregnancy, and less than 2% in women with nonprimary infection. There is no licensed CMV vaccine. Good hygiene practices and avoiding intimate contact with young children (e.g., kissing on the mouth and sharing utensils) have been suggested as an approach to prevent maternal primary CMV infection during pregnancy, but remains an unproven method of reducing the risk of congenital CMV infection. Approximately 1 in 10 infants who acquire CMV in utero will have clinical signs at birth, and an additional 10 to 15% will go on to develop late-onset sequelae. Antiviral treatment prenatally and postnatally has not proven effective at preventing congenital or postnatal CMV infection, and is not recommended for routine clinical care. However, antiviral treatment when initiated in the first month of life for symptomatic congenital CMV infection is recommended for improved neurodevelopmental and audiologic outcomes. Birth Defects Research 109:336-346, 2017. © 2017 Wiley Periodicals, Inc.
Differential Coupling of the Sphingosine 1-Phosphate Receptors Edg-1, Edg-3, and H218/Edg-5 to the Gi, Gq, and G12 Families of Heterotrimeric G Proteins (1999)

Sphingosine 1-phosphate (S1P) is one of several bioactive phospholipids that exert profound mitogenic and morphogenic actions. Originally characterized as a second messenger, S1P is now recognized to achieve many of its effects through cell surface, G protein-coupled receptors. We used a subunit-selective [(35)S]GTPgammaS binding assay to investigate whether the variety of actions exerted through Edg-1, a recently identified receptor for S1P, might be achieved through multiple G proteins. We found, employing both Sf9 and HEK293 cells, that Edg-1 activates only members of the G(i) family, and not G(s), G(q), G(12), or G(13). We additionally established that Edg-1 activates G(i) in response not only to S1P but also sphingosylphosphorylcholine; no effects of lysophosphatidic acid through Edg-1 were evident. Our assays further revealed a receptor(s) for S1P endogenous to HEK293 cells that mediates activation of G(13) as well as G(i). Because several of the biological actions of S1P are assumed to proceed through the G(12/13) family, we tested whether Edg-3 and H218/Edg-5, two other receptors for S1P, might have a broader coupling profile than Edg-1. Indeed, Edg-3 and H218/Edg-5 communicate not only with G(i) but also with G(q) and G(13). These studies represent the first characterization of S1P receptor activity through G proteins directly and establish fundamental differences in coupling.
TLB consistency on highly-parallel shared-memory multiprocessors (1988)

Multiprocessors that store the same shared data in different caches must ensure that these caches have consistent copies. Almost all known solutions to this cache consistency problem are only suitable for architectures with a few tens of processing elements (PEs). Efficient solutions to the TLB (translation lookaside buffer) consistency problem, a special case of the cache consistency problem, can be found for highly parallel, shared-memory multiprocessors (HPSMMs) with many hundreds of PEs for the following reasons: the number of references to address translation information per modification is very large; the cache for storing translation information can be present anywhere on the path from the PEs to memory; when the memory mapping needs to be modified, one can often select which translation information to change; and obsolete mapping information can be used until permanent changes must be made. Three general methods are presented that use these features and can be used on HPSMMs to maintain TLB consistency. Some interesting issues inherent to the TLB consistency problem and the support of a demand-paged virtual memory system on an HPSMM are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Device dependent control of chemical-mechanical polishing of dielectric films (2000)

This paper presents a control scheme for run-to-run control of chemical-mechanical polishing (CMP). The control scheme tracks both device pattern dependent and equipment induced disturbances. The structure of the controller is such that sensitivity to qual (unpatterned blanket oxide) wafer frequency is minimized. Additionally, prethickness variation and metrology delay are accounted for in the design. Results from applying this scheme in volume production are presented.
Noonan syndrome, the <i>SOS1</i> gene and embryonal rhabdomyosarcoma (2010)

Noonan Syndrome (NS) is an autosomal dominant condition characterized by short stature, facial dysmorphisms, and congenital heart defects, and is caused by mutations in either PTPN11, KRAS, NRAS, SHOC2, RAF1, or SOS1. Furthermore, NS is known for its predisposition to develop cancer, particularly hematological malignancies and specific solid tumors, mainly neuroblastoma and embryonal rhabdomyosacroma (ERMS). Until recently, however, cancer predisposition in NS patients with SOS1 mutations was not reported. Here we present a NS patient with a de novo germline SOS1 mutation (p.Lys728Ile) and ERMS. This heterozygous germline mutation was homozygously present in the ERMS of this patient due to an acquired uniparental disomy (UPD) of chromosome 2. In addition, several other chromosomal aberrations were encountered, some of which are known to recurrently occur in ERMS. Sequence analysis of the SOS1 gene in 20 sporadic ERMS tumors failed to reveal any pathogenic mutations, implicating that SOS1 is not a major player in the development of this tumor outside the context of NS.
DNS performance and the effectiveness of caching (2002)

This paper presents a detailed analysis of traces of domain name system (DNS) and associated TCP traffic collected on the Internet links of the MIT Laboratory for Computer Science and the Korea Advanced Institute of Science and Technology (KAIST). The first part of the analysis details how clients at these institutions interact with the wide-area domain name system, focusing on client-perceived performance and the prevalence of failures and errors. The second part evaluates the effectiveness of DNS caching. In the most recent MIT trace, 23% of lookups receive no answer; these lookups account for more than half of all traced DNS packets since query packets are retransmitted overly persistently. About 13% of all lookups result in an answer that indicates an error condition. Many of these errors appear to be caused by missing inverse (IP-to-name) mappings or NS records that point to nonexistent or inappropriate hosts. 27% of the queries sent to the root name servers result in such errors. The paper also presents the results of trace-driven simulations that explore the effect of varying time-to-live (TTL) and varying degrees of cache sharing on DNS cache hit rates. Due to the heavy-tailed nature of name accesses, reducing the TTL of address (A) records to as low as a few hundred seconds has little adverse effect on hit rates, and little benefit is obtained from sharing a forwarding DNS cache among more than 10 or 20 clients. These results suggest that client latency is not as dependent on aggressive caching as is commonly believed, and that the widespread use of dynamic low-TTL A-record bindings should not greatly increase DNS related wide-area network traffic.
High-resolution Mapping of Linear Antibody Epitopes Using Ultrahigh-density Peptide Microarrays (2012)

Antibodies empower numerous important scientific, clinical, diagnostic, and industrial applications. Ideally, the epitope(s) targeted by an antibody should be identified and characterized, thereby establishing antibody reactivity, highlighting possible cross-reactivities, and perhaps even warning against unwanted (e.g. autoimmune) reactivities. Antibodies target proteins as either conformational or linear epitopes. The latter are typically probed with peptides, but the cost of peptide screening programs tends to prohibit comprehensive specificity analysis. To perform high-throughput, high-resolution mapping of linear antibody epitopes, we have used ultrahigh-density peptide microarrays generating several hundred thousand different peptides per array. Using exhaustive length and substitution analysis, we have successfully examined the specificity of a panel of polyclonal antibodies raised against linear epitopes of the human proteome and obtained very detailed descriptions of the involved specificities. The epitopes identified ranged from 4 to 12 amino acids in size. In general, the antibodies were of exquisite specificity, frequently disallowing even single conservative substitutions. In several cases, multiple distinct epitopes could be identified for the same target protein, suggesting an efficient approach to the generation of paired antibodies. Two alternative epitope mapping approaches identified similar, although not necessarily identical, epitopes. These results show that ultrahigh-density peptide microarrays can be used for linear epitope mapping. With an upper theoretical limit of 2,000,000 individual peptides per array, these peptide microarrays may even be used for a systematic validation of antibodies at the proteomic level.
Understanding and using hyaluronic acid (2004)

Injectable synthetic hyaluronic acid is biodegradable and biocompatible with human hyaluronic acid. Soft tissue augmentation with this product does not require prior skin testing and provides robust augmentation. Its ability to attract and maintain significant amounts of water accounts for the volume and duration of correction that persists until the stabilized gel is completely metabolized.
Topographic probes of angiotensin and receptor: potent angiotensin II agonist containing diphenylalanine and long-acting antagonists containing biphenylalanine and 2-indan amino acid in position 8 (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTopographic probes of angiotensin and receptor: potent angiotensin II agonist containing diphenylalanine and long-acting antagonists containing biphenylalanine and 2-indan amino acid in position 8Kun Hwa Hsieh, Thomas R. LaHann, and Robert C. SpethCite this: J. Med. Chem. 1989, 32, 4, 898–903Publication Date (Print):April 1, 1989Publication History Published online1 May 2002Published inissue 1 April 1989https://pubs.acs.org/doi/10.1021/jm00124a028https://doi.org/10.1021/jm00124a028research-articleACS PublicationsRequest reuse permissionsArticle Views265Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Nonlinear Filter System for Beautifying Face Images with Enhancement Using Interactive Evolutionary Computing (2006)

An image processing system for beautifying human face images with enhancement is proposed using interactive evolutionary computing. Authors proposed before a nonlinear digital filter bank system, which removes undesirable skin components, such as wrinkles and spots, from human face images to make the face look beautified. Also, the system is designed optimally on the basis of human subjective criteria using interactive evolutionary computing. The system was shown to be almost effective, but it slightly blurs the input face image. Here, face image beautifying system with image enhancement is proposed. This system can beautify face images while keeping them sharp. Moreover, this system can sharpen blurred face images. The whole system is also designed by the interactive evolutionary computing. The new system is verified to be effective in processing an actual face image.
Pathogenic mutations inactivate parkin by distinct mechanisms (2004)

Loss of parkin function is the major cause of autosomal recessive Parkinson's disease (ARPD). A wide variety of parkin mutations have been identified in patients; however, the pathophysiological mechanisms leading to the inactivation of mutant parkin are poorly understood. In this study we characterized pathogenic C- and N-terminal parkin mutants and found distinct pathways of parkin inactivation. Deletion of the C terminus abrogated the association of parkin with cellular membranes and induced rapid misfolding and aggregation. Four N-terminal missense mutations, located within the ubiquitin-like domain (UBL), decrease the stability of parkin; as a consequence, these mutants are rapidly degraded by the proteasome. Furthermore, we present evidence that a smaller parkin species of 42 kDa, which is present in extracts prepared from human brain and cultured cells, originates from an internal start site and lacks the N-terminal UBL domain.
Intravascular Optical-Resolution Photoacoustic Tomography with a 1.1 mm Diameter Catheter (2014)

Photoacoustic imaging is an emerging technology that can provide anatomic, functional, and molecular information about biological tissue. Intravascular spectroscopic and molecular photoacoustic imaging can potentially improve the identification of atherosclerotic plaque composition, the detection of inflammation, and ultimately the risk stratification of atherosclerosis. In this study, a first-of-its-kind intravascular optical-resolution photoacoustic tomography (OR-PAT) system with a 1.1 mm diameter catheter is developed, offering optical-diffraction limited transverse resolution as fine as 19.6 μm, ∼ 10-fold finer than that of conventional intravascular photoacoustic and ultrasonic imaging. To offer complementary imaging information and depth, the system also acquires co-registered intravascular ultrasound images in parallel. Imaging of an iliac stent and a lipid phantom shows that the high resolution and contrast of OR-PAT can enable improved stent implantation guidance and lipid identification. In the future, these capabilities may ultimately improve the diagnosis and interventional treatment of vulnerable atherosclerotic plaques, which are prone to cause thrombotic complications such as myocardial infarction and stroke.
Characterization of a membrane‐associated phosphotyrosyl protein phosphatase from the A431 human epidermoid carcinoma cell line (1989)

The A431 human epidermoid carcinoma cell line exhibits a 30-100-fold overexpression of the epidermal growth factor (EGF) receptor. We have characterized a membrane-associated phosphotyrosyl-protein phosphatase (PTPase) in these cells since it seemed reasonable that overexpression of the EGF-receptor tyrosine kinase will be matched by high PTPase activity. Indeed, of 12 cell lines tested, the A431 cells had the highest specific PTPase activity. About 70% of the total cellular PTPase activity was found associated with membranes after cell fractionation. The membrane-associated PTPase was hydrophobic as judged by its behaviour in Triton X-114 phase partitioning. High-performance liquid chromatography (HPLC) on a DEAE column revealed a single, homogeneous species of membrane-associated PTPase with an apparent molecular mass of 43 kDa as determined by HPLC on a gel permeation column in the presence of Triton X-100. Comparison of this PTPase with the membrane-associated PTPase activities present in rat spleen and in the human chronic myelogenous leukemia cell line K562 revealed additional species resolvable by DEAE-HPLC. These findings suggest that cells may possess different PTPase activities depending on their growth and differentiation states.
Injectable microcapsules prepared with biodegradable poly(α-hydroxy) acids for prolonged release of drugs (1997)

In this paper, microencapsulation techniques for the preparation of drug-containing monolithic microcapsules for prolonged release using biodegradable poly(alpha-hydroxy) acids, such as polylactic acid, poly(lactide-co-glycolide) and copoly(lactic/glycolic) acid are reviewed. Phase separation, solvent evaporation, and spray drying procedures are discussed. In order to achieve controlled-release formulations of highly water-soluble drugs that are entrapped efficiently, various manufacturing techniques and procedures have been developed. Degradation of poly(alpha-hydroxy) acids is altered by the copolymer ratio and molecular weight of the polymer used to make microcapsules and the amounts of released microencapsulated drugs correlate almost linearly with polymer degradation, indicating that controlled-release formulations, which release drugs over different times, can be prepared using suitable poly(alpha-hydroxy) acids with different degradation rates.
Functional STAT 1 and 3 signaling by the leptin receptor (OB-R); reduced expression of the rat fatty leptin receptor in transfected cells (1996)

The leptin receptor (OB-R) bears homology to members of the class I cytokine receptor family. We demonstrate that leptin binding to OB-R stimulates formation of STAT-1 and STAT-3 complexes, thereby defining transcriptional motifs for genes that are under leptin control. Transfected fa OB-R bound leptin with equal affinity to that of wild type OB-R. fa OB-R abundance was about 7 fold reduced compared to control cells. Surprisingly, the low level of fa OB-R is fully capable of activating the STAT signal transduction pathway. We discuss plausible explanations for the obese phenotype in Zucker fatty rats.