gebhart/patrepeval huggingface dataset:
Utilization of DNA as a Sole Source of Phosphorus, Carbon, and Energy by <i>Shewanella</i> spp.: Ecological and Physiological Implications for Dissimilatory Metal Reduction (2007)

The solubility of orthophosphate (PO4(3-)) in iron-rich sediments can be exceedingly low, limiting the bioavailability of this essential nutrient to microbial populations that catalyze critical biogeochemical reactions. Here we demonstrate that dissolved extracellular DNA can serve as a sole source of phosphorus, as well as carbon and energy, for metal-reducing bacteria of the genus Shewanella. Shewanella oneidensis MR-1, Shewanella putrefaciens CN32, and Shewanella sp. strain W3-18-1 all grew with DNA but displayed different growth rates. W3-18-1 exhibited the highest growth rate with DNA. While strain W3-18-1 displayed Ca2+-independent DNA utilization, both CN32 and MR-1 required millimolar concentrations of Ca2+ for growth with DNA. For S. oneidensis MR-1, the utilization of DNA as a sole source of phosphorus is linked to the activities of extracellular phosphatase(s) and a Ca2+-dependent nuclease(s), which are regulated by phosphorus availability. Mass spectrometry analysis of the extracellular proteome of MR-1 identified one putative endonuclease (SO1844), a predicted UshA (bifunctional UDP-sugar hydrolase/5' nucleotidase), a predicted PhoX (calcium-activated alkaline phosphatase), and a predicted CpdB (bifunctional 2',3' cyclic nucleotide 2' phosphodiesterase/3' nucleotidase), all of which could play important roles in the extracellular degradation of DNA under phosphorus-limiting conditions. Overall, the results of this study suggest that the ability to use exogenous DNA as the sole source of phosphorus is widespread among the shewanellae, and perhaps among all prokaryotes, and may be especially important for nutrient cycling in metal-reducing environments.
Dos Barcelonas para un mar: La transformación de la cooperación ambiental en el Mediterráneo. (2008)

La cooperacion para la proteccion ambiental del Mediterraneo esta en proceso de transformacion. Por decirlo en pocas palabras: pueden estar convergiendo (sin llegar a fusionarse) los dos marcos que hasta ahora han protagonizado estos esfuerzos, esto es, el auspiciado desde 1975 por el Programa de Naciones Unidas para el Medio Ambiente (PNUMA) y la dimension ambiental del Proceso de Barcelona. Y esta transformacion tiene el potencial de desencadenar cambios en los objetivos, los mecanismos y las redes de actores relevantes para la conservacion de esta zona del planeta, al mismo tiempo ecologicamente vulnerable y vulnerada.
Optimal quantum circuit synthesis from controlled-unitary gates (2004)

Using a geometric approach, we derive the minimum number of applications needed for an arbitrary controlled-unitary gate to construct a universal quantum circuit. An analytic construction procedure is presented and shown to be either optimal or close to optimal. This result can be extended to improve the efficiency of universal quantum circuit construction from any entangling gate. In addition, for both the controlled-NOT (CNOT) and double-CNOT gates, we develop simple analytic ways to construct universal quantum circuits with three applications, which is the least possible for these gates.
Changes in Insulin Sensitivity in Response to Troglitazone Do Not Differ Between Subjects With and Without the Common, Functional Pro12Ala Peroxisome Proliferator–Activated Receptor-γ2 Gene Variant (2004)

Among young Hispanic women at high risk for type 2 diabetes, the Pro12Ala variant of the PPAR-gamma receptor gene did not explain the failure of approximately 1/3 of subjects to increase their insulin sensitivity when placed on troglitazone at a dose of 400 mg/day.
Targeting of lysosomes by liposomes modified with octadecyl-rhodamine B (2011)

The use of lysosome-targeted liposomes may significantly improve a delivery of therapeutic enzymes into lysosomes for the treatment of lysosome-associated diseases. The aim of this research was to achieve a specific intracellular targeting of lysosomes, by using liposomes modified with the lysosomotropic octadecyl-rhodamine B (RhB) and loaded with a model compound, fluorescein isothiocyanate (FITC)-dextran (FD). Plain and RhB-modified liposomes were prepared by hydration of lipid films and loaded with FD or with 5-dodecanoylaminofluorescein di-β-d-galactopyranoside (C(12)FDG), a specific substrate for the intralysosomal β-galactosidase. The delivery of these liposomes and their content to lysosomes in HeLa cells was investigated by confocal microscopy, flow cytometry, and subcellular fractionation. Confocal microscopy demonstrated that RhB-liposomes co-localize well with the specific lysosomal markers, unlike plain liposomes. The comparison of the FITC fluorescence of the lysosomes isolated by subcellular fractionation also showed that the efficiency of FD delivery into lysosomes by RhB-modified liposomes was significantly higher compared with plain liposomes. These results were additionally confirmed by the flow cytometry of the intact cells treated with C(12)FDG-loaded liposomes that also demonstrated increased lysosomal targeting by RhB-modified liposomes. The modification of the liposomal surface with a lysosomotropic ligand, such as octadecyl-RhB, can significantly increase the delivery of liposomal loads to lysosomes.
<i>Ancylostoma caninum</i> MTP-1, an Astacin-Like Metalloprotease Secreted by Infective Hookworm Larvae, Is Involved in Tissue Migration (2006)

Infective larvae (L3) of nematodes secrete macromolecules that are critical to infection and establishment of the parasite in the host. The dog hookworm Ancylostoma caninum secretes an astacin-like metalloprotease, Ac-MTP-1, upon activation in vitro with host serum. Recombinant Ac-MTP-1 was expressed in the baculovirus/insect cell system as a secreted protein and was purified from culture medium by two separate methods, cation-exchange fast-performance liquid chromatography and gelatin-affinity chromatography. Recombinant MTP-1 was catalytically active and digested a range of native and denatured connective tissue substrates, including gelatin, collagen, laminin, and fibronectin. A dog was immunized with recombinant Ac-MTP-1 formulated with AS03 adjuvant, and the antiserum was used to immunolocalize the anatomic sites of expression within A. caninum L3 to secretory granules in the glandular esophagus and the channels that connect the esophagus to the L3 surface and to the cuticle. Antiserum inhibited the ability of recombinant MTP-1 to digest collagen by 85% and inhibited larval migration through tissue in vitro by 70 to 75%, in contrast to just 5 to 10% inhibition obtained with preimmunization serum. The metalloprotease inhibitors EDTA and 1,10-phenanthroline also reduced the penetration of L3 through skin in vitro by 43 to 61%. The data strongly suggest that Ac-MTP-1 is critical for the invasion process of hookworm larvae, and moreover, that antibodies against the enzyme can neutralize its function and inhibit migration.
The N terminus of interleukin-8 (IL-8) receptor confers high affinity binding to human IL-8 (1994)

Interleukin-8 (IL-8) is a potent inflammatory mediator that belongs to the family of C-X-C chemokines. IL-8 promotes the activation and the extravasation of circulating neutrophils to the site of inflammation. Two IL-8 receptor isotypes (type A and B) are identified in human and rabbit neutrophils. IL-8 receptors belongs to the superfamily of G-protein-coupled receptors. Both receptor subtypes A and B bind with high affinity to human IL-8, but they exhibit distinct binding affinity to two functional and structurally related IL-8 peptides, melanoma growth-stimulating activity peptide (MGSA) and neutrophil-activating peptide-2 (NAP-2). Human IL-8 receptor A binds with low affinity to MGSA or NAP-2. In contrast, human IL-8 receptor B binds MGSA with high affinity, and NAP-2 with lesser affinity. Using receptor subtype chimeras, we determined that the N-terminal domain of the receptor confers ligand binding specificity (LaRosa, G. J., Thomas, K. M., Kaufmann, M. E., Mark, R., White, M., Taylor, L., Gray, G., Witt, D., and Navarro, J. (1992) J. Biol. Chem. 267, 25402-25406). In this work, we characterized by molecular cloning and expression a mouse receptor structurally homologous to the IL-8 receptor. We isolated a clone by screening a mouse genomic library with a rabbit IL-8 receptor A cDNA fragment as a probe. The mouse clone exhibited an open reading frame encoding a 359-amino acid protein. Hydropathy plot analysis of the amino acid sequence reveals seven transmembrane domains characteristic of G-protein-coupled receptors. The N terminus and the second extracellular loop contain one and two putative N-glycosylation sites, respectively. The intracellular C-terminal tail contains Ser and Thr residues as potential phosphorylation sites. Northern blot analysis showed that the mouse receptor gene is expressed in mouse neutrophils. The mouse receptor shows 65, 74, 66, and 70% amino acid identity to the rabbit IL-8 receptor subtypes A and B and human IL-8 receptor subtypes A and B, respectively. However, neither mouse neutrophils nor CHO cells expressing the mouse receptor bind human IL-8 in the nanomolar range. To identify the domain(s) conferring high affinity binding to IL-8, we constructed rabbit/mouse receptor chimeras.(ABSTRACT TRUNCATED AT 400 WORDS)
Simulation and Measurement of On-Chip Supply Noise in Multi-Gigabit I/O Interfaces (2008)

Characteristics of the on-chip power supply noise in a 6.4 Gbps serial link interface test system are analyzed by both simulation and measurement techniques. Pre- and post-layout simulation methodologies are discussed with different on-chip power grid modeling approaches proposed and supply current profile extraction method established. An on-chip supply noise measurement technique is introduced to allow monitoring both the statistics and dynamics of supply noise. Good agreement between simulation results and measurement results from the test system transmitting PRBS7 data pattern at 6.4 Gbps are observed in time and frequency domain.
Early detection of sudden cardiac death by using classical linear techniques and time-frequency methods on electrocardiogram signals (2011)

Early detection of sudden cardiac death may be used for surviving the life of cardiac patients. In this paper we have investigated an algorithm to detect and predict sudden cardiac death, by processing of heart rate variability signal through the classical and time-frequency methods. At first, one minute of ECG signals, just before the cardiac death event are extracted and used to compute heart rate variability (HRV) signal. Five features in time domain and four features in frequency domain are extracted from the HRV signal and used as classical linear features. Then the Wigner Ville transform is applied to the HRV signal, and 11 extra features in the time-frequency (TF) domain are obtained. In order to improve the performance of classification, the principal component analysis (PCA) is applied to the obtained features vector. Finally a neural network classifier is applied to the reduced features. The obtained results show that the TF method can classify normal and SCD subjects, more efficiently than the classical methods. A MIT-BIH ECG database was used to evaluate the proposed method. The proposed method was implemented using MLP classifier and had 74.36% and 99.16% correct detection rate (accuracy) for classical features and TF method, respectively. Also, the accuracy of the KNN classifier were 73.87% and 96.04%.
[Preparative isolation of individual tRNA Phe by high performance liquid reversed-phase chromatography]. (1988)

A method of rapid preparative isolation of Phe-tRNA(Phe) (E. coli) of almost 100% purity (1800 pmoles per 1 OE260) is developed. In includes three successive chromatographies of total tRNA on C18-modified Lichrosphere Si-1000 in acetonitrile gradients. Other individual tRNAs can be isolated by the same approach.
Novel Highly Conductive and Transparent Graphene‐Based Conductors (2012)

Transparent conductors based on few-layer graphene (FLG) intercalated with ferric chloride (FeCl(3)) have an outstandingly low sheet resistance and high optical transparency. FeCl(3)-FLGs outperform the current limit of transparent conductors such as indium tin oxide, carbon-nanotube films, and doped graphene materials. This makes FeCl(3)-FLG materials the best transparent conductor for optoelectronic devices.
Epidemiological characteristics and the entire evolution of coronavirus disease 2019 in Wuhan, China (2020)

The number of new cases has dropped significantly after the government taking the isolation of four types of personnel and the community containment for 14 days. Our results indicate that the mortality and proportion of severe and critical cases gradually decreased over time, and critical and dead cases are more incline to be older individuals.
ChemInform Abstract: Peptide Amidation by Enzymatic Transacylation and Photolysis. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The evolution of 5GHz WLAN toward higher throughputs (2003)

A standardization effort has started within the IEEE 802.11 working group to define the next generation of 802.11 wireless LANs. This article illustrates how throughput achieved above the MAC layer of 5 GHz WLANs can be increased from an existing 30 Mb/s maximum with 802.11a/g to rates exceeding 90 Mb/s. After a brief review of ongoing WLAN standardization activities, the support of a higher physical-layer bit rate by various standardized MAC protocols (802.11, 802.11e, and HIPERLAN/2) is discussed, showing the PHY and MAC layers must be considered jointly in order to achieve a significant throughput increase. Various physical layer techniques are compared in terms of performance and complexity. In particular, simulations show that by relying on MAC layers with good efficiency like 802.11e and HIPERLAN/2, a combination of space-time block coding with a possibility of channel bundling could bring a peak throughput increase from 30 to 90 Mb/s as well as a significant cell range increase.
Tapping vs. circling selections on pen-based devices (2004)

Tapping-based selection methods for handheld devices may need to be supplemented with other approaches as increasingly complex tasks are carried out using those devices. Circling selection methods (such as the Lasso) allow users to select objects on a touch screen by circling with a pen. An experimental comparison of the selection time and accuracy between a circling method and a traditional tapping style of selection was carried out. The experiment used a two dimensional grid (varying in terms of the sizes and the distances of the targets). Analysis of variance showed that tapping selection time differed significantly depending on the size and spacing of the targets. In contrast, circling selection times differed significantly for different levels of target cohesiveness and shape complexity. The results are discussed in terms of implications for design of new pen-based selection methods for handheld devices, and also in terms of evaluation methodology for input selection methods.
Allogeneic hematopoietic stem cell transplantation in Fanconi anemia: the European Group for Blood and Marrow Transplantation experience (2013)

Although allogeneic hematopoietic stem cell transplantation (HSCT) remains the only curative treatment for patients with Fanconi anemia (FA), published series mostly refer to single-center experience with limited numbers of patients. We analyzed results in 795 patients with FA who underwent first HSCT between May 1972 and January 2010. With a 6-year median follow-up, overall survival was 49% at 20 years (95% confidence interval, 38-65 years). Better outcome was observed for patients transplanted before the age of 10 years, before clonal evolution (ie, myelodysplastic syndrome or acute myeloid leukemia), from a matched family donor, after a conditioning regimen without irradiation, the latter including fludarabine. Chronic graft-versus-host disease and secondary malignancy were deleterious when considered as time-dependent covariates. Age more than 10 years at time of HSCT, clonal evolution as an indication for transplantation, peripheral blood as source of stem cells, and chronic graft-versus-host disease were found to be independently associated with the risk for secondary malignancy. Changes in transplant protocols have significantly improved the outcome of patients with FA, who should be transplanted at a young age, with bone marrow as the source of stem cells.
Nanoporous Si as an Efficient Thermoelectric Material (2008)

Room-temperature thermoelectric properties of n-type crystalline Si with periodically arranged nanometer-sized pores are computed using a combination of classical molecular dynamics for lattice thermal conductivity and ab initio density functional theory for electrical conductivity, Seebeck coefficient, and electronic contribution to the thermal conductivity. The electrical conductivity is found to decrease by a factor of 2-4, depending on doping levels, compared to that of bulk due to confinement. The Seebeck coefficient S yields a 2-fold increase for carrier concentrations less than 2 x 10(19) cm(-3), above which S remains closer to the bulk value. Combining these results with our calculations of lattice thermal conductivity, we predict the figure of merit ZT to increase by 2 orders of magnitude over that of bulk. This enhancement is due to the combination of the nanometer size of pores which greatly reduces the thermal conductivity and the ordered arrangement of pores which allows for only a moderate reduction in the power factor. We find that while alignment of pores is necessary to preserve power factor values comparable to those of bulk Si, a symmetric arrangement is not required. These findings indicate that nanoporous semiconductors with aligned pores may be highly attractive materials for thermoelectric applications.
Harmonic voltage measurements using CVTs (2005)

A method for harmonic measurements in EHV systems is proposed that is based on the measurement of currents in capacitor voltage transformers (CVT) and values of capacitor elements of CVTs. This is the basis of a new technique, which uses current sensors installed within CVTs. Simulation studies and field tests have confirmed the validity and practicability of the technique. In the new method, the input terminal voltage is directly determined and no information about transfer function and burden is required. In order to verify the field test results, errors in the CVT output voltage recorded during tests were compensated by the use of the transfer function determined independently in transfer function tests for the same burden. A very good correlation was observed between the two results. The results of field measurements are also presented.
Kinetics and Mechanism of Bacterial Disinfection by Chlorine Dioxide (1967)

Survival data are presented for a fecal strain of Escherichia coli exposed to three concentrations of chlorine dioxide at four temperatures. Chick's first-order reaction equation is generalized to a pseudo nth-order model. Nonlinear least squares curve-fitting of the survival data to the nth order model was performed on an analogue computer. The data were observed to follow fractional order kinetics with respect to survival concentration, with an apparent activation energy of 12,000 cal/mole. Initial experiments support the thesis that the mechanism of chlorine dioxide kill occurs via disruption of protein synthesis.
Total synthesis of the Cephalotaxus alkaloids. Problem in nucleophilic aromatic substitution (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of the Cephalotaxus alkaloids. Problem in nucleophilic aromatic substitutionM. F. Semmelhack, B. P. Chong, R. D. Stauffer, T. D. Rogerson, A. Chong, and Lawrence D. JonesCite this: J. Am. Chem. Soc. 1975, 97, 9, 2507–2516Publication Date (Print):April 1, 1975Publication History Published online1 May 2002Published inissue 1 April 1975https://pubs.acs.org/doi/10.1021/ja00842a031https://doi.org/10.1021/ja00842a031research-articleACS PublicationsRequest reuse permissionsArticle Views1665Altmetric-Citations143LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
THE SMOOTH MUSCLE CELL (1971)

Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk in culture, microfibrils (110 A) appeared within the spaces between the layers of cells. Basement membrane-like material also appeared adjacent to the cells. Analysis of the microfibrils showed that they have an amino acid composition similar to that of the microfibrillar protein of the intact elastic fiber. These investigations coupled with the radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell.
Primary Virus Envelope Cross-Reactivity of the Broadening Neutralizing Antibody Response during Early Chronic Human Immunodeficiency Virus Type 1 Infection (1999)

To test the hypothesis that changing neutralizing antibody responses against human immunodeficiency virus type 1 (HIV-1) during chronic infection were a response to emergence of neutralization escape mutants, we cloned expressed and characterized envelope clones from patients in the Multicenter AIDS Cohort Study (MACS). Pseudotyped HIV-1 envelope clones obtained from differing time points were assessed for sensitivity to neutralization by using sera from different times from the same and different patients. Clones from early and late time points during chronic infection had similar neutralization sensitivity, and neutralizing antibody responses cross-reacted with early, late, and heterologous envelopes. The potential for broadly effective HIV-1 immunization is supported.
Comparison of DNA methylation profiles in human fetal and adult red blood cell progenitors (2015)

Our DNA methylome maps confirm the widespread dynamic changes in DNA methylation that occur during human erythropoiesis. These changes tend to happen near erythroid enhancers, further highlighting their importance in erythroid regulation and HbF production. Finally, DNA methylation may act independently of the transcription factor BCL11A to repress fetal hemoglobin production. This provides cues on strategies to more efficiently re-activate HbF production in sickle cell disease and β-thalassemia patients.