gebhart/patrepeval huggingface dataset:
Antibody-based enzyme-linked immunosorbent assay for determination of anti-PGL-I specific circulating immune complex in leprosy patients (1999)

A serological study was performed in 122 individuals: 75 leprosy patients and 47 healthy controls. The ELISA test was performed for IgG and IgM using the glycolipid PGL-I antigen from Mycobacterium leprae. Circulating immune complexes (CIC) were isolated by PEG 6000 precipitation method and after dissociation with an acid solution, the IgG and IgM specific against PGL-I were tested with the ELISA test. The multibacillary patients had high levels of antibodies, compared with paucibacillary patients and controls. The antibodies isolated from the CIC presented a similar spectrum spectral distribution as the serology. A positive correlation between the levels of free and CIC bound antibodies was observed. In contrast with tuberculosis patients, specific antibodies present in CIC were not responsible for false-negative results found in some multibacillary patients' serology, since no or very low levels of specific antibodies were found in PEG precipitated serum of these patients. No relation was observed with specific antibody levels detected in CIC during leprosy reactions.
The duration, magnitude and compartmentalization of ERK MAP kinase activity: mechanisms for providing signaling specificity (2005)

ERK MAP kinase signaling plays a pivotal role in diverse cellular functions, including cell proliferation, differentiation, migration and survival. One of the central questions concerning this signaling is how activation of the same protein kinase, ERK, elicits distinct cellular outcomes. Recent progress has demonstrated that differences in the duration, magnitude and subcellular compartmentalization of ERK activity generate variations in signaling output that regulate cell fate decisions. Furthermore, several molecules have been identified as spatial, temporal or strength-controlling regulators of ERK activity. Signaling by various extracellular stimuli thus could be modulated by these regulators to give qualitative and quantitative differences in ERK activity, which are then interpreted by the cells as determinants for appropriate responses.
Analysis of the Herpesvirus Chemokine-binding Glycoprotein G Residues Essential for Chemokine Binding and Biological Activity (2008)

Viral chemokine-binding protein (vCKBP) are expressed by large DNA viruses, such as herpesviruses and poxviruses. vCKBP can bind chemokines with high affinity and efficiently neutralize their ability to induce cell migration. Recently, herpesvirus glycoprotein G (gG) was identified as a member of the vCKBP-4 subfamily. The structural domains of gG important for binding to chemokines and biological activity, however, are unknown. Here, we used equine herpesvirus type 1 (EHV-1) as a model to determine residues in EHV-1 gG that are involved in the processes of chemokine binding and interaction with target cells. First, comprehensive analysis of glycosylation of EHV-1 gG revealed that N-glycosylation is not required for binding of gG to chemokines but is essential for biological activity of the protein. Second, the epitope responsible for the binding to chemokines was localized to 40 amino acids in the hypervariable region (amino acids 301-340) of the protein. Third, hybrid molecules, designed as loss- and gain-of-function gG proteins, were engineered. In these hybrid glycoproteins the hypervariable regions of EHV-1 gG, a vCKBP, and the closely related EHV-4 gG, which does not display any chemokine binding capabilities, were exchanged. gG variants containing the EHV-1 hypervariable region were able to bind chemokines and were biologically active, whereas hybrid gGs containing the corresponding region of EHV-4 gG were not. Taking these results together, this report is the first to provide insight into the functional residues of an alphaherpesviral vCKBP.
TFSOI-can it meet the challenge of single chip portable wireless systems? (2002)

Tremendous progress in understanding and improving SOI material and devices has been made in recent years. The reduced junction capacitance and the minimal body-effect of SOI provides an inherent advantage for low-voltage low-power applications. Recent growth in the portable wireless communication market and the push for low cost solutions have driven the semiconductor industry into a race for the ultimate solution: a single chip radio capable of handling both the RF and baseband functions. In this paper, the applications of Thin-Film-Silicon-On-Insulator (TFSOI) to the different circuit elements of wireless communication systems are reviewed. The use of CMOS SOI devices as well as future development essential to realize the single chip radio are discussed.
Integration, transcription, and control of a Drosophila heat shock gene in mouse cells. (1981)

Mouse L cells were transformed with a cloned 3.6-kilobase (kb) segment of Drosophila melanogaster DNA carrying the 2.25-kb transcribed sequence for the Drosophila 70,000-dalton heat shock protein (hsp70) and 1.1 kb and 0.2 kb of 5' and 3' flanking DNA, respectively. Heat shock of one of three such transformed cell lines containing multiple copies of the intact Drosophila segment induced the abundant accumulation of transcripts of the Drosophila gene, with correct or nearly correct 5' and 3' termini. This provides evidence, in accord with earlier indications, that diverse eukaryotes, including vertebrates, have heat shock systems similar to that studied extensively in Drosophila. Our results suggest that the signals for heat shock transcription and the chromosomal sites with which they interact have been highly conserved in evolution and that the regulatory sequences controlling transcription of the gene for hsp70 lie within the 3.6-kb Drosophila segment.
Profiling Heparin–Chemokine Interactions Using Synthetic Tools (2007)

Glycosaminoglycans (GAGs), such as heparin or heparan sulfate, are required for the in vivo function of chemokines. Chemokines play a crucial role in the recruitment of leukocyte subsets to sites of inflammation and lymphocytes trafficking. GAG-chemokine interactions mediate cell migration and determine which leukocyte subsets enter tissues. Identifying the exact GAC sequences that bind to particular chemokines is key to understand chemokine function at the molecular level and develop strategies to interfere with chemokine-mediated processes. Here, we characterize the heparin binding profiles of eight chemokines (CCL21, IL-8, CXCL12, CXCL13, CCL19, CCL25, CCL28, and CXCL16) by employing heparin microarrays containing a small library of synthetic heparin oligosaccharides. The chemokines differ significantly in their interactions with heparin oligosaccharides: While some chemokines, (e.g., CCL21) strongly bind to a hexasaccharide containing the GlcNSO3(6-OSO3)-IdoA(2-OSO3) repeating unit, CCL19 does not bind and CXCL12 binds only weakly. The carbohydrate microarray binding results were validated by surface plasmon resonance experiments. In vitro chemotaxis assays revealed that dendrimers coated with the fully sulfated heparin hexasaccharide inhibit lymphocyte migration toward CCL21. Migration toward CXCL12 or CCL19 was not affected. These in vitro homing assays indicate that multivalent synthetic heparin dendrimers inhibit the migration of lymphocytes toward certain chemokine gradients by blocking the formation of a chemokine concentration gradient on GAG endothelial chains. These findings are in agreement with preliminary in vivo measurements of circulating lymphocytes. The results presented here contribute to the understanding of GAG-chemokine interactions, a first step toward the design of novel drugs that modulate chemokine activity.
I/O for Virtual Machine Monitors: Security and Performance Issues (2008)

Modern I/O architectures are quite complex, so keeping a virtual machine monitor (VMM), or hypervisor, small is difficult. Many current hypervisors move the large, complex, and sometimes proprietary device drivers out of the VMM into one or more partitions, leading to inherent problems in complexity, security, and performance.
Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range (1972)

Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.
Dual Aromatase−Steroid Sulfatase Inhibitors (2007)

By introducting the steroid sulfatase inhibitory pharmacophore into aromatase inhibitor 1 (YM511), two series of single agent dual aromatase-sulfatase inhibitors (DASIs) were generated. The best DASIs in vitro (JEG-3 cells) are 5, (IC50(aromatase) = 0.82 nM; IC50(sulfatase) = 39 nM), and 14, (IC50(aromatase) = 0.77 nM; IC50(sulfatase) = 590 nM). X-ray crystallography of 5, and docking studies of selected compounds into an aromatase homology model and the steroid sulfatase crystal structure are presented. Both 5 and 14 inhibit aromatase and sulfatase in PMSG pretreated adult female Wistar rats potently 3 h after a single oral 10 mg/kg dose. Almost complete dual inhibition is observed for 5 but the levels were reduced to 85% (aromatase) and 72% (sulfatase) after 24 h. DASI 5 did not inhibit aldosterone synthesis. The development of a potent and selective DASI should allow the therapeutic potential of dual aromatase-sulfatase inhibition in hormone-dependent breast cancer to be assessed.
Predisposition to nevirapine hypersensitivity associated with HLA-DRB1*0101 and abrogated by low CD4 T-cell counts (2004)

Genetic (human leukocyte antigen), disease-related and demographic risk factors for nevirapine reactions were examined in a nevirapine-exposed cohort. Cases involving combinations of hepatitis, fever or rash were associated with an interaction between HLA-DRB1*0101 and the percentage of CD4, whereas no associations were detected for isolated rash. These data suggest that HLA-DRB1*0101 and the CD4 status may determine susceptibility to nevirapine hypersensitivity, consistent with a CD4 T-cell-dependent immune response to nevirapine-specific antigens.
Vaccinia Virus Infection during Murine Pregnancy: a New Pathogenesis Model for Vaccinia Fetalis (2004)

Vaccinia fetalis, the vertical transfer of vaccinia virus from mother to fetus, is a relatively rare but often fatal complication of primary vaccinia virus vaccination during pregnancy. To date there has been no attempt to develop an animal model to study the pathogenesis of this acute viral infection in vivo. Here we report that infection of gestating BALB/c mice by either intravenous or intraperitoneal routes with the Western Reserve strain of vaccinia virus results in the rapid colonization of the placenta and vertical transfer of virus to the developing fetus. Systemic maternal infections during gestation lead to the death of all offspring prior to or very shortly after birth. Using in situ hybridization for vaccinia virus mRNA to identify infected cells, we show that the virus initially colonizes cells lining maternal lacunae within the trophospongium layer of the placenta. The study of this model will significantly enhance our understanding of the pathogenesis of fetal vaccinia virus infections and aid in the development of effective treatments designed to reduce the risk of vaccinia virus-associated complications during pregnancy.
<i>Melanocortin 1 receptor</i> genotype: an important determinant of the damage response of melanocytes to ultraviolet radiation (2010)

The melanocortin 1 receptor gene is a main determinant of human pigmentation, and a melanoma susceptibility gene, because its variants that are strongly associated with red hair color increase melanoma risk. To test experimentally the association between melanocortin 1 receptor genotype and melanoma susceptibility, we compared the responses of primary human melanocyte cultures naturally expressing different melanocortin 1 receptor variants to α-melanocortin and ultraviolet radiation. We found that expression of 2 red hair variants abolished the response to α-melanocortin and its photoprotective effects, evidenced by lack of functional coupling of the receptor, and absence of reduction in ultraviolet radiation-induced hydrogen peroxide generation or enhancement of repair of DNA photoproducts, respectively. These variants had different heterozygous effects on receptor function. Microarray data confirmed the observed differences in responses of melanocytes with functional vs. nonfunctional receptor to α-melanocortin and ultraviolet radiation, and identified DNA repair and antioxidant genes that are modulated by α-melanocortin. Our findings highlight the molecular mechanisms by which the melanocortin 1 receptor genotype controls genomic stability of and the mutagenic effect of ultraviolet radiation on human melanocytes.
Self-Calibration Algorithm for a Pressure Sensor with a Real-Time Approach Based on an Artificial Neural Network (2018)

This paper presents a novel approach to predicting self-calibration in a pressure sensor using a proposed Levenberg Marquardt Back Propagation Artificial Neural Network (LMBP-ANN) model. The self-calibration algorithm should be able to fix major problems in the pressure sensor such as hysteresis, variation in gain and lack of linearity with high accuracy. The traditional calibration process for this kind of sensor is a time-consuming task because it is usually done through manual and repetitive identification. Furthermore, a traditional computational method is inadequate for solving the problem since it is extremely difficult to resolve the mathematical formula among multiple confounding pressure variables. Accordingly, this paper describes a new self-calibration methodology for nonlinear pressure sensors based on an LMBP-ANN model. The proposed method was achieved using a collected dataset from pressure sensors in real time. The load cell will be used as a reference for measuring the applied force. The proposed method was validated by comparing the output pressure of the trained network with the experimental target pressure (reference). This paper also shows that the proposed model exhibited a remarkable performance than traditional methods with a max mean square error of 0.17325 and an R-value over 0.99 for the total response of training, testing and validation. To verify the proposed model's capability to build a self-calibration algorithm, the model was tested using an untrained input data set. As a result, the proposed LMBP-ANN model for self-calibration purposes is able to successfully predict the desired pressure over time, even the uncertain behaviour of the pressure sensors due to its material creep. This means that the proposed model overcomes the problems of hysteresis, variation in gain and lack of linearity over time. In return, this can be used to enhance the durability of the grasping mechanism, leading to a more robust and secure grasp for paralyzed hands. Furthermore, the exposed analysis approach in this paper can be a useful methodology for the user to evaluate the performance of any measurement system in a real-time environment.
THERMOGENESIS AND OBESITY (1981)

Journal Article THERMOGENESIS AND OBESITY Get access W P T JAMES, MA MD FRCP, W P T JAMES, MA MD FRCP University of Cambridge and Medical Research Council, Dunn Clinical Nutrition Centre, Addenbrooke's HospitalCambridge Search for other works by this author on: Oxford Academic PubMed Google Scholar P TRAYHURN, DPhil P TRAYHURN, DPhil University of Cambridge and Medical Research Council, Dunn Clinical Nutrition Centre, Addenbrooke's HospitalCambridge Search for other works by this author on: Oxford Academic PubMed Google Scholar British Medical Bulletin, Volume 37, Issue 1, January 1981, Pages 43–48, https://doi.org/10.1093/oxfordjournals.bmb.a071674 Published: 01 January 1981
Audio Production Techniques for Video (2023)

Audio Production Techniques for Video (1987) looks at the mechanics and operation of audio tape and video tape recorders; audio and video synchronization-technology; studio and location audio production processes; video and audio post-production; troubleshooting time-code problems.
Top of the world (2008)

Paul Catherall's architectural linocuts are rapidly becoming stars in the firmament of great British design. Clare Barry looks at the artist's career to date
Citrullinated proteins in rheumatoid arthritis: Crucial … but not sufficient! (2006)

Anti–citrullinated protein antibodies (ACPAs)are highly specific for rheumatoid arthritis (RA) andhave thus become part of the diagnostic armamentariumin inflammatory arthritis. Circumstantial clinical evi-dence also suggests that ACPAs may participate inimportant pathophysiologic processes in RA. This con-cept has recently been supported by the specific gene–environment interaction between smoking and HLA–DR4 in ACPA-positive but not ACPA-negative patientswith RA and by the enhancement of tissue injury byACPAs in experimental arthritis. In parallel, importantprogress has been made in the detection and identifica-tion of citrullinated proteins as potential targets forACPAs in inflamed synovium as well as other tissue.However, it becomes increasingly clear that well-definedcitrullinated epitopes, rather than the mere presence ofcitrullinated proteins as such, may be relevant for theinduction of ACPAs and, eventually, for the pathogenic-ity of anticitrulline reactivity. Therefore, defining theclinically relevant citrullinated epitopes and experimen-tally assessing the requirements for optimal and coordi-nated immune activation by these epitopes are 2 majorchallenges in this field.
Intra Block Copy in HEVC Screen Content Coding Extensions (2016)

With the emerging applications such as online gaming and Wi-Fi display, screen content video, including computer generated text, graphics and animations, becomes more popular than ever. Traditional video coding technologies typically were developed based on models that fit into natural, camera-captured video. The distinct characteristics exhibited between these two types of contents necessitate the exploration of coding efficiency improvement given that new tools can be developed specially for screen content video. The HEVC Screen Content Coding Extensions (HEVC SCC) have been developed to incorporate such new coding tools in order to achieve better compression efficiency. In this paper, intra block copy (IBC, or intra picture block compensation), also named current picture referencing (CPR) in HEVC SCC, is introduced and discussed. This tool is very efficient for coding of screen content video in that repeated patterns in text and graphics rich content occur frequently within the same picture. Having a previously reconstructed block with equal or similar pattern as a predictor can effectively reduce the prediction error and therefore improve coding efficiency. Simulation results show that up to 50% BD rate reduction in all intra coding can be achieved with intra block copy enabled, compared to the HEVC reference encoder without this tool. Significant BD rate reductions for other coding configurations are also observed.
Embryonic stem cells neural differentiation qualifies the role of Wnt/β-Catenin signals in human telencephalic specification and regionalization (2013)

Wnt-ligands are among key morphogens that mediate patterning of the anterior territories of the developing brain in mammals. We qualified the role of Wnt-signals in regional specification and subregional organization of the human telencephalon using human pluripotent stem cells (hPSCs). One step neural conversion of hPSCs using SMAD inhibitors leads to progenitors with a default rostral identity. It provides an ideal biological substrate for investigating the role of Wnt signaling in both anteroposterior and dorso-ventral processes. Challenging hPSC-neural derivatives with Wnt-antagonists, alone or combined with sonic hedgehog (Shh), we found that Wnt-inhibition promote both telencephalic specification and ventral patterning of telencephalic neural precursors in a dose-dependent manner. Using optimal Wnt-antagonist and Shh-agonist signals we produced human ventral-telencephalic precursors, committed to differentiation into striatal projection neurons both in vitro and in vivo after homotypic transplantation in quinolinate-lesioned rats. This study indicates that sequentially organized Wnt-signals play a key role in the development of human ventral telencephalic territories from which the striatum arise. In addition, the optimized production of hPSC-derived striatal cells described here offers a relevant biological resource for exploring and curing Huntington disease.
Against democracy: literary experience in the era of emancipations (2013)

This book argues that we can no longer envision a political system that might practically displace democracy or, more accurately, global democratic state capitalism. Democracy has become fundamental: It extends deeper and deeper into everyday life; it grounds and limits our political thought and values. That is the sense in which we do indeed live at history's end. But this end is not a happy one, because the system that we now have does not satisfy tests that we can legitimately put to it. In this situation, it is important to come to new terms with the fact that literature, at least until about 1945, was predominantly hostile to political democracy. Literature's deep-seated conservative, counterdemocratic tendencies, along with its capacity to make important distinctions among political, cultural, and experiential democracies and its capacity to uncover hidden, nonpolitical democracies in everyday life, is now a resource not just for cultural conservatives but for all those who take a critical attitude toward the current political, cultural, and economic structures. Literature, and certain novelists in particular, helps us not so much to imagine social possibilities beyond democracy as to understand how life might be lived both in and outside democratic state capitalism. Drawing on political theory, intellectual history, and the techniques of close reading, Against Democracy offers new accounts of the ethos of refusing democracy, of literary criticism's contribution to that ethos, and of the history of conservatism, as well as innovative interpretations of a range of writers, including Tocqueville, Disraeli, George Eliot, E. M. Forster, and Saul Bellow.
Regulation of the Neurofibromatosis Type 2 Tumor Suppressor Protein, Merlin, by Adhesion and Growth Arrest Stimuli (1998)

The neurofibromatosis type 2 tumor suppressor gene is inactivated in the development of familial and sporadic schwannomas and meningiomas. The encoded protein, Merlin, is closely related to the Ezrin, Radixin, and Moesin family of membrane/cytoskeletal linker proteins. Examination of Merlin in several cell lines revealed that the protein migrates as two distinct species near 70 kDa. Phosphatase treatment and orthophosphate labeling demonstrated that the species with decreased mobility is phosphorylated. Given Merlin's localization to cortical actin structures, we examined the effect of cell-cell contact or other forms of growth arrest on Merlin expression and post-translational modification. Under conditions of confluency or serum deprivation, the levels of phosphorylated and unphosphorylated Merlin species increased significantly. Cells arrested in G1 by other methods or other phases of the cell cycle did not show changes in Merlin levels. Furthermore, loss of adhesion resulted in a nearly complete dephosphorylation of Merlin, which was reversed upon re-plating of cells, suggesting Merlin phosphorylation may be responsive to cell spreading or changes in cell shape. Thus, the tumor suppressor function of Merlin may involve the regulation of cellular responses to cues such as cell-cell contact, growth factor microenvironment, or changes in cell shape.