gebhart/patrepeval huggingface dataset:
Oligosaccharide-protein conjugate vaccines induce and prime for oligoclonal IgG antibody responses to the Haemophilus influenzae b capsular polysaccharide in human infants. (1986)

The diversity of the IgG antibody induced by immunization of human infants and children with conjugate vaccines, composed of oligosaccharides prepared from the Haemophilus influenzae b capsular polysaccharide (CP) and covalently linked to diphtheria toxoids, was studied by analytical IEF. The antibody response was similar, in the degree of restriction, to that observed in the antibody response of older children to immunization with the CP alone. The booster responses induced by reimmunization with conjugate vaccines were accompanied by increases predominantly in the IgG antibody clonotypes expressed after the priming dose of vaccine. After a series of conjugate immunizations, immunization with isolated CP boosted the antibody titer and increased expression from all the clonotypes that were expressed after conjugate immunization. These findings suggest that the conjugate vaccines are acting on a limited number of human B cell clones that are preferentially restimulated after reimmunization. Little evidence of antigen-specific B cell recruitment was found. In addition, the ability of isolated CP immunization to restimulate the same B cell clone indicates that the responding B cell has matured and suggests a linear rather than a dual developmental pathway for the B cell participating in this human antibody response.
Improving locality with parallel hierarchical copying GC (2006)

This paper shows how to reduce cache and TLB misses by changing the order in which a parallel garbage collector copies heap objects. Reducing cache and TLB misses improves program run time. Parallel garbage collection improves scaling on multi-processor machines. Technology trends indicate that both memory locality and multi-processor scaling increase in importance. Our new algorithmis based on the earlier single-threaded "hierarchical decomposi-tion" algorithm by Wilson, Lam, and Moher. This paper presents a thorough evaluation of parallel hierarchical copying, showing that it improves spatial locality, reduces cache and TLB misses, and speeds up 14 out of 26 benchmarks.
Single-crystal nanopyramidal BGaN by nanoselective area growth on AlN/Si(111) and GaN templates (2016)

We report nano-selective area growth (NSAG) of BGaN by MOCVD on AlN/Si(111) and GaN templates resulting in 150 nm single crystalline nanopyramids. This is in contrast to unmasked or micro-selective area growth, which results in a multi-crystalline structure on both substrates. Various characterization techniques were used to evaluate NSAG as a viable technique to improve BGaN material quality on AlN/Si(111) using results of GaN NSAG and unmasked BGaN growth for comparison. Evaluation of BGaN nanopyramid quality, shape and size uniformity revealed that the growth mechanism is the same on both the templates. Further STEM analysis of BGaN nanopyramids on AlN/Si (111) templates confirmed that these are single-crystalline structures without any dislocations, likely due to single nucleation occurring in the 80 nm mask opening. CL results correspond to boron content between 1.7% and 2.0% in the nanopyramids. We conclude that NSAG is promising for growth of high-quality BGaN nanostructures and complex nano-heterostructures, especially for low-cost silicon substrates.
Progress in n.m.r. zeugmatographic imaging (1980)

Applications of nuclear magnetic resonance (n.m.r.) zeugmatographic imaging to medical diagnosis and to medical, physiological, and biological research require the development of appropriate imaging instrumentation and ancillary techniques, as well as an understanding of the biological significance of the imaging results. A whole body imaging system, relying primarily upon reconstruction from projections, is under development in the expectation that the reconstruction approach will be the most practical one for many purposes. In addition, injectable magnetic reagents that can selectively change tissue water relaxation times and image contrast are under development so as to increase the specificity and versatility of the measurements. If very high magnetic fields are employed, 31P n.m.r. zeugmatography may be practical at very low resolution for human diagnostic studies and for experiments on perfused organs and small animals. Preliminary images, showing the spatial distributions of different phosphorus metabolites in the compartments of test objects, have been obtained at 146 MHz by reconstruction techniques.
Planar simplification and texturing of dense point cloud maps (2013)

Dense RGB-D based SLAM techniques and high-fidelity LIDAR scanners are examples from an abundant set of systems capable of providing multi-million point datasets. These large datasets quickly become difficult to process and work with due to the sheer volume of data, which typically contains significant redundant information, such as the representation of planar surfaces with hundreds of thousands of points. In order to exploit the richness of information provided by dense methods in real-time robotics, techniques are required to reduce the inherent redundancy of the data. In this paper we present a method for efficient triangulation and texturing of planar surfaces in large point clouds. Experimental results show that our algorithm removes more than 90% of the input planar points, leading to a triangulation with only 10% of the original amount of triangles per planar segment, improving upon an existing planar simplification algorithm. Despite the large reduction in vertex count, the principal geometric features of each segment are well preserved. In addition to this, our texture generation algorithm preserves all colour information contained within planar segments, resulting in a visually appealing and geometrically accurate simplified representation.
Enhanced binding of TBK1 by an optineurin mutant that causes a familial form of primary open angle glaucoma (2008)

TANK-binding kinase 1 (TBK1) was identified as a binding partner for Optineurin (OPTN) in two-hybrid screens, an interaction confirmed by overexpression/immunoprecipitation experiments in HEK293 cells and by coimmunoprecipitation of endogenous OPTN and TBK1 from cell extracts. A TBK1 binding site was located between residues 1-127 of OPTN, residues 78-121 displaying striking homology to the TBK1-binding domain of TANK. The OPTN-binding domain was localised to residues 601-729 of TBK1, while TBK1[1-688] which cannot bind to TANK, did not interact with OPTN. The OPTN[E50K] mutant associated with Primary Open Angle Glaucoma (POAG) displayed strikingly enhanced binding to TBK1, suggesting that this interaction may contribute to familial POAG caused by this mutation.
FACILE PREPARATION AND SOME APPLICATIONS OF AN AFFINITY MATRIX WITH A CLEAVABLE CONNECTOR ARM CONTAINING A DISULFIDE BOND (1987)

A versatile affinity matrix in which the ligand of interest is linked to the matrix through a connector arm containing a disulfide bond is described. It can be synthesized from any amino-substituted matrix by successive reaction with 2-imino-thiolane, 5,5'-dithiobis(2-nitrobenzoic acid), and a thiol derivative of the ligand of choice. The repertoire of ligands can be significantly increased by the appropriate use of avidin-biotin bridges. After adsorption of the material to be fractionated, elution can be effected by reducing the disulfide bond in the connector arm with dithiothreitol. Examples of the preparation and use of various affinity matrices based on amino-substituted Sepharose 6MB are given. One involves the immobilization of the Fab' fragment of a monoclonal antibody against Aspergillus oryzae beta-galactosidase and the specific binding of that enzyme to the resulting immunoaffinity matrix. Another involves the immobilization of N-biotinyl-2-thioethylamine followed by complex formation with avidin. The resulting avidin-substituted matrix was used for the selective adsorption and subsequent recovery of mouse hybridoma cells producing anti-avidin antibodies. By further complexing the avidin-substituted matrix with appropriate biotinylated antigens, it should be possible to fractionate cells producing antibodies against a variety of antigens.
A high-speed multiplier using a redundant binary adder tree (1987)

A 16-bit /spl times/ 16-bit multiplier for 2 two's-complement binary numbers based on a new algorithm is described. This multiplier has been fabricated on an LSI chip using a standard n-E/D MOS process technology with a 2.7-/spl mu/m design rule. This multiplier is characterized by use of a binary tree of redundant binary adders. In the new algorithm, n-bit multiplication is performed in a time proportional to log/SUB 2/ n and the physical design of the multiplier is constructed of a regular cellular array. This new algorithm has been proposed by N. Takagi et al. (1982, 1983). The 16-bit/spl times/16-bit multiplier chip size is 5.8 /spl times/ 6.3 mm/SUP 2/ using the new layout for a binary adder tree. The chip contains about 10600 transistors, and the longest logic path includes 46 gates. The multiplication time was measured as 120 ns. It is estimated that a 32-bit /spl times/ 32-bit multiplication time is about 140 ns.
1,2- and 1,7-Dicarba-closo-dodecaborane(12)-iridium(I) complexes formed through metal-carbon .sigma.-bonds. Synthesis and characterization. Unusual stereochemistry of the oxidative addition reaction of hydrogen (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1,2- and 1,7-Dicarba-closo-dodecaborane(12)-iridium(I) complexes formed through metal-carbon .sigma.-bonds. Synthesis and characterization. Unusual stereochemistry of the oxidative addition reaction of hydrogenB. Longato, F. Morandini, and S. BresadolaCite this: Inorg. Chem. 1976, 15, 3, 650–656Publication Date (Print):March 1, 1976Publication History Published online1 May 2002Published inissue 1 March 1976https://pubs.acs.org/doi/10.1021/ic50157a031https://doi.org/10.1021/ic50157a031research-articleACS PublicationsRequest reuse permissionsArticle Views73Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Preparation and Physical Properties of MDEA-based Polyurethane Cationomers and their Application to Textile Coatings (2007)

Cationic aqueous polyurethane (CAPU) coatings were prepared by the acetone process using 4,4-methylene bis (isocyantocyclohexane) (H 12 MDI), N-methyldiethyolamine (N-MDEA) and a series of various polydiols (polyethylene glycol (PEG), polypropylene glycol (PPG) and polytetramethylene glycol (PTMG) for ether-type polyols and polycaprolactone (PCL) for ester-type polyol) with different molecular weights (M n =1000, 2000 and 3000 g/mole). The surface tension of CAPU dispersion, contact angle, thermal properties of CAPU casting film and its tensile strength were examined. The waterproof (WP) and water vapor permeability (WVP) of coated fabric and its washing durability were also studied. Our results showed the ether-based CAPU showed a higher surface tension value, contact angle and WVP with a sequence of PEG-PU &gt; PTMG-PU &gt; PPG-PU &gt; PCL-PU, and it increased with increasing M n of the soft segment. As for the thermal properties, the PEG-PU had the highest glass transition temperatures of the soft segment (Tg s ) and melting point (Tm s ) than those of PCL -PU, PTMG-PU and PPG-PU. For the thermal stability of CAPUs casting film, the PEG-PU had ideal thermal stability, followed by PTMG-PU and PCL -PU, and the CAPU with a larger M n of the soft segment had better thermal stability. The PTMG-PU had excellent tensile strength, followed by PCL -PU and PPG-PU. For coated fabric, the trend of WVP followed the order: PEG-PU &gt; PTMG-PU &gt; PPG-PU &gt; PCL -PU, while the WP followed the order: PTMG-PU &gt; PCLPU &gt; PPG-PU &gt; PEG-PU. Finally, the CAPUcoated fabric had an excellent performance after washing and its wash-durability was still 80 %.
Lactobacilli Differentially Modulate Expression of Cytokines and Maturation Surface Markers in Murine Dendritic Cells (2002)

Dendritic cells (DC) play a pivotal immunoregulatory role in the Th1, Th2, and Th3 cell balance and are present throughout the gastrointestinal tract. Thus, DC may be targets for modulation by gut microbes, including ingested probiotics. In the present study, we tested the hypothesis that species of Lactobacillus, important members of the gut flora, differentially activate DC. Bone marrow-derived murine DC were exposed to various lethally irradiated Lactobacillus spp. and resultant culture supernatants were analyzed for IL-6, IL-10, IL-12, and TNF-alpha. Substantial differences were found among strains in the capacity to induce IL-12 and TNF-alpha production in the DC. Similar but less pronounced differences were observed among lactobacilli in the induction of IL-6 and IL-10. Although all strains up-regulated surface MHC class II and B7-2 (CD86), which is indicative of DC maturation, those lactobacilli with greatest capacity to induce IL-12 were most effective. Remarkably, Lactobacillus reuteri DSM12246, a poor IL-12 inducer, inhibited IL-12, IL-6, and TNF-alpha induction by the otherwise strong cytokine inducer L. casei CHCC3139, while IL-10 production remained unaltered. In analogous fashion, L. reuteri reduced L. casei-induced up-regulation of B7-2. These results suggest that different species of Lactobacillus exert very different DC activation patterns and, furthermore, at least one species may be capable of inhibiting activities of other species in the genus. Thus, the potential exists for Th1/Th2/Th3-driving capacities of the gut DC to be modulated according to composition of gut microflora, including ingested probiotics.
Acid and neutral sphingomyelinases: roles and mechanisms of regulation (2004)

Ceramide, an emerging bioactive lipid and second messenger, is mainly generated by hydrolysis of sphingomyelin through the action of sphingomyelinases. At least two sphingomyelinases, neutral and acid sphingomyelinases, are activated in response to many extracellular stimuli. Despite extensive studies, the precise cellular function of each of these sphingomyelinases in sphingomyelin turnover and in the regulation of ceramide-mediated responses is not well understood. Therefore, it is essential to elucidate the factors and mechanisms that control the activation of acid and neutral sphingomyelinases to understand their the roles in cell regulation. This review will focus on the molecular mechanisms that regulate these enzymes in vivo and in vitro, especially the roles of oxidants (glutathione, peroxide, nitric oxide), proteins (saposin, caveolin 1, caspases), and lipids (diacylglycerol, arachidonic acid, and ceramide).
Coordinate Expression of Transcriptionally Regulated Isocitrate Lyase and Malate Synthase Genes in Brassica napus L. (1989)

Lucio Comai, Robert A. Dietrich, Daniel J. Maslyar, Catherine S. Baden, John J. Harada, Coordinate Expression of Transcriptionally Regulated Isocitrate Lyase and Malate Synthase Genes in Brassica napus L., The Plant Cell, Vol. 1, No. 3 (Mar., 1989), pp. 293-300
The mouse albumin enhancer contains a negative regulatory element that interacts with a novel DNA-binding protein. (1990)

The far-upstream mouse albumin enhancer (-10.5 to -8.43 kilobases) has both positive and negative regulatory domains which contribute to the rate and tissue specificity of albumin gene transcription. (R. S. Herbst, N. Friedman, J. E. Darnell, Jr., and L. E. Babiss, Proc. Natl. Acad. Sci. USA 86:1553-1557). In this work, the negative regulatory region has been functionally localized to sequences -8.7 to -8.43 kilobases upstream of the albumin gene cap site. In the absence of the albumin-modulating region (in which there are binding sites for the transcription factor C/EBP), the negative region can suppress a neighboring positive-acting element, thereby interfering with albumin enhancer function. The negative region is also capable of negating the positive action of the heterologous transthyretin enhancer in an orientation-independent fashion. Within this negative-acting region we can detect two DNA-binding sites, both of which are recognized by a protein present in all cell types tested. This DNA-binding activity is not competed for by any of a series of known DNA-binding sites, and hence this new protein is a candidate for a role in suppressing the albumin gene in nonhepatic cells.
Improving patient outcomes with regenerative medicine: How the Regenerative Medicine Manufacturing Society plans to move the needle forward in cell manufacturing, standards, 3D bioprinting, artificial intelligence-enabled automation, education, and training (2020)

The Regenerative Medicine Manufacturing Society (RMMS) is the first and only professional society dedicated toward advancing manufacturing solutions for the field of regenerative medicine. RMMS's vision is to provide greater patient access to regenerative medicine therapies through innovative manufacturing solutions. Our mission is to identify unmet needs and gaps in regenerative medicine manufacturing and catalyze the generation of new ideas and solutions by working with private and public stakeholders. We aim to accomplish our mission through outreach and education programs and securing grants for public-private collaborations in regenerative medicine manufacturing. This perspective will cover four impact areas that the society's leadership team has identified as critical: (a) cell manufacturing and scale-up/out, respectively, for allogeneic and autologous cell therapies, (b) standards for regenerative medicine, (c) 3D bioprinting, and (d) artificial intelligence-enabled automation. In addition to covering these areas and ways in which the society intends to advance the field in a collaborative nature, we will also discuss education and training. Education and training is an area that is critical for communicating the current challenges, developing solutions to accelerate the commercialization of the latest technological advances, and growing the workforce in the rapidly expanding sector of regenerative medicine.
The role of B7-CD28 co-stimulation in tumor rejection (1998)

The role of B7 co-stimulatory signaling in in vivo tumor rejection remains incompletely characterized. In particular, the relative competence of B7-1 (CD80) and B7-2 (CD86) to provide effective co-stimulus is not well defined, and the identification of the T cell co-stimulatory receptor that mediates B7 co-stimulation in tumor rejection has not been addressed. These issues were studied by assessing rejection of B7-negative or B7-transfected tumor cells in CD28-expressing or CD28-deficient hosts. B7-negative EL4 tumor cells grew progressively in normal syngeneic C57BUL6 (B6) mice. In contrast EL4 cells transfected with either full length B7-1 or full length B7-2 were rejected, indicating that both B7-1 and B7-2 are competent to mediate rejection of EL4 tumor cells. Expression of truncated B7-1 or B7-2 products, with complete deletion of cytoplasmic domains, was as effective as expression of full length B7-1 or B7-2 in mediating rejection. In contrast to the rejection of B7-transfected EL4 cells observed in CD28-expressing syngeneic hosts, B7-1- and B7-2-positive EL4 cells as well as control EL4 cells grew progressively in CD28-deficient mice, demonstrating the requirement for host expression of CD28 in B7-mediated tumor rejection. These results indicate that interaction of host CD28 with co-stimulatory extracellular B7-1 or B7-2 ligands expressed on tumor cells can play a necessary role in mediating tumor rejection.
The Limits of Bloodwarming (1997)

An inline microwave bloodwarmer may be used to heat blood safely to 49 degrees C. Blood warmed to this temperature may significantly increase the amount of heat returned to the hypothermic trauma patient.
Stability and neutralization capacity of a novel mosaic HIV-1 gp140 trimer in a guinea pig model (2012)

The generation of globally relevant HIV-1 immunogens mimicking native, trimeric Envelope (Env) structure, remains a major challenge for HIV-1 vaccine development. We identified a mosaic Env sequence, originally designed to optimize cellular immunologic coverage of global HIV-1 sequence diversity, which was capable of forming biochemically stable trimers. We assessed this mosaic Env gp140 trimer in guinea pig immunogenicity studies compared to our previously reported biochemically stable C97ZA012 (clade C) trimer.
Striatal binding of the PET ligand <sup>11</sup>C‐raclopride is altered by drugs that modify synaptic dopamine levels (1993)

Bilateral decreases in striatal 11C-raclopride binding were observed in adult female baboons with high resolution PET following administration of drugs that act centrally on dopaminergic neurons. At baseline and following administration of d-amphetamine (a dopamine-releasing drug), GBR-12909 (a potent dopamine reuptake inhibitor), or tetrabenazine (a biogenic amine depleting drug) PET scans of 11C-raclopride binding were obtained in a CTI 931 positron tomograph. In all studies, the ratio of the distribution volumes for the striatum to the cerebellum for 11C-raclopride binding decreased significantly by an average of 16.2% for d-amphetamine, 22.1% for GBR-12909, and 28.3% for tetrabenazine while there were no significant changes observed in the cerebellum or in the rate of systemic metabolism of the radiotracer. These decreases exceed the test/retest variability of striatal 11C-raclopride binding measured in the same animals under identical experimental conditions (Dewey et al., 1992b). Together these studies demonstrate that PET measurements of striatal 11C-raclopride binding can be used to indirectly and non-invasively monitor changes in synaptic dopamine concentrations that result from a variety of neurophysiologic mechanisms.