gebhart/patrepeval huggingface dataset:
Target-specific drug release to the colon (2008)

A microbially controlled system, which is a well-accepted approach, based on natural polymers, has the greatest potential for colonic delivery, particularly in terms of site specificity and safety. However, close attention should be paid to the performance of these products in the heterogeneous environment of the human gastrointestinal tract.
Characterization of the<scp>d</scp>-Xylulose 5-Phosphate/<scp>d</scp>-Fructose 6-Phosphate Phosphoketolase Gene (<i>xfp</i>) from<i>Bifidobacterium lactis</i> (2001)

A D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase (Xfp) from the probiotic Bifidobacterium lactis was purified to homogeneity. The specific activity of the purified enzyme with D-fructose 6-phosphate as a substrate is 4.28 Units per mg of enzyme. K(m) values for D-xylulose 5-phosphate and D-fructose 6-phosphate are 45 and 10 mM, respectively. The native enzyme has a molecular mass of 550,000 Da. The subunit size upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis (90,000 Da) corresponds with the size (92,529 Da) calculated from the amino acid sequence of the isolated gene (named xfp) encoding 825 amino acids. The xfp gene was identified on the chromosome of B. lactis with the help of degenerated nucleotide probes deduced from the common N-terminal amino acid sequence of both the native and denatured enzyme. Comparison of the deduced amino acid sequence of the cloned gene with sequences in public databases revealed high homologies with hypothetical proteins (26 to 55% identity) in 20 microbial genomes. The amino acid sequence derived from the xfp gene contains typical thiamine diphosphate (ThDP) binding sites reported for other ThDP-dependent enzymes. Two truncated putative genes, pta and guaA, were localized adjacent to xfp on the B. lactis chromosome coding for a phosphotransacetylase and a guanosine monophosphate synthetase homologous to products of genes in Mycobacterium tuberculosis. However, xfp is transcribed in B. lactis as a monocistronic operon. It is the first reported and sequenced gene of a phosphoketolase.
Analytical Strategies in Metabonomics (2006)

To perform metabonomics investigations, it is necessary to generate comprehensive metabolite profiles for complex samples such as biofluids and tissue/tissue extracts. Analytical technologies that can be used to achieve this aim are constantly evolving, and new developments are changing the way in which such profiles' metabolite profiles can be generated. Here, the utility of various analytical techniques for global metabolite profiling, such as, e.g., 1H NMR, MS, HPLC-MS, and GC-MS, are explored and compared.
Synthesis and antiviral activity of water-soluble esters of acyclovir [9-[(2-hydroxyethoxy)methyl]guanine) (1983)

Several water-soluble ester derivatives of acyclovir [9-[(2-hydroxyethoxy)methyl]guanine], i.e., the 2'-O-glycyl-, 2'-O-alpha-alanyl-, 2'-O-beta-alanyl- and 2'-O-3-carboxypropionyl esters, were synthesized and evaluated for their antiviral activity in cell culture. The compounds were all prepared directly from acyclovir by application of the usual esterification methods with the appropriate acyl precursors and isolated as their hydrochloride or sodium salts. When assayed in primary rabbit kidney cell cultures against various herpes simplex virus type 1 and type 2 strains, the four acyclovir esters proved almost as active as acyclovir itself, suggesting that they were readily hydrolyzed to release the parent compound.
New Capability for Remote Controlled Excavation (1989)

&lt;div class="htmlview paragraph"&gt;Remote controlled operation of construction equipment has been state-of-the-art for some years. The availability of remote controls which have been designed and developed for general use on commercial machines is a recent development that is the subject of this paper. The John Deere teleoperated excavator represents a new capability that is now available to the construction industry for use on construction sites that preclude the on-site presence of human operators. This paper will describe the basic machine, the controls, vision system and integration of the remote control adjunct to the operational system. Much of the development of the initial capability was done with the cooperation of Vectran Corporation of Pittsburgh, Pennsylvania.&lt;/div&gt;
Sports cars versus freight trains: Why infusion performance is in the details * (2007)

University of Chicago, Department of Anesthesia and Critical Care, Chicago, IL The authors have not disclosed any potential conflicts of interest. *See also p. 2792.
E-cadherin gene mutations provide clues to diffuse type gastric carcinomas. (1994)

The calcium-dependent homophilic cell adhesion molecule and candidate suppressor gene, E (epithelial)-cadherin, plays a major role in the organization and integrity of most epithelial tissues. Diffusely growing gastric carcinomas show markedly reduced homophilic cell-to-cell interactions. We speculated that mutations in the E-cadherin gene may be responsible for the scattered phenotype of this type of carcinoma. For that reason we have examined E-cadherin in 26 diffuse type, 20 intestinal type and 7 mixed gastric carcinomas (Laurén's classification) at the DNA, RNA, and protein levels. Reverse transcription polymerase chain reaction and direct sequencing of amplified E-cadherin complementary DNA fragments revealed inframe skipping of either exon 8 or exon 9 in 10 patients with diffuse tumors and an exon 9 deletion in one patient with a mixed carcinoma; both exons encode putative calcium binding domains. These alterations were not seen in nontumorous gastric tissues. Splice site mutations responsible for the exon deletions were identified in six of these patients, eliminating the possibility of alternative splicing mechanisms. Five of these splice site alterations were confirmed as somatic mutations. Non-splice site mutations were observed in three diffuse type tumors, namely a 69-base pair deletion of exon 10 and two point mutations, one of which destroys a putative calcium binding region. Immunohistochemical evaluation showed E-cadherin immunoreactivity in tumors and lymph node metastases of patients expressing abnormal mRNA. The allelic status of the E-cadherin gene was analyzed in one patient, revealing loss of heterozygosity with retention of a mutated E-cadherin allele. Overall, E-cadherin mutations were identified in 50% (13 of 26) of the diffuse type and in 14% (1 of 7) of the mixed carcinomas. In contrast, two silent E-cadherin mutations (not changing the amino acid sequence) were detected in two tumors of the intestinal type. Our study provides strong in vivo evidence that E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas and support a tumor/metastasis suppressor gene hypothesis.
Vitamin C Inhibits NF-κB Activation by TNF Via the Activation of p38 Mitogen-Activated Protein Kinase (2000)

The transcription factor NF-kappaB is a central mediator of altered gene expression during inflammation, and is implicated in a number of pathologies, including cancer, atherosclerosis, and viral infection. We report in this study that vitamin C inhibits the activation of NF-kappaB by multiple stimuli, including IL-1 and TNF in the endothelial cell line ECV304 and in primary HUVECs. The induction of a NF-kappaB-dependent gene, IL-8, by TNF was also inhibited. The effect requires millimolar concentrations of vitamin C, which occur intracellularly in vivo, particularly during inflammation. Vitamin C was not toxic to cells, did not inhibit another inducible transcription factor, STAT1, and had no effect on the DNA binding of NF-kappaB. Inhibition by vitamin C was not simply an antioxidant effect, because redox-insensitive pathways to NF-kappaB were also blocked. Vitamin C was shown to block IL-1- and TNF-mediated degradation and phosphorylation of I-kappaBalpha (inhibitory protein that dissociates from NF-kappaB), due to inhibition of I-kappaB kinase (IKK) activation. Inhibition of TNF-driven IKK activation was mediated by p38 mitogen-activated protein kinase, because treatment of cells with vitamin C led to a rapid and sustained activation of p38, and the specific p38 inhibitor SB203580 reversed the inhibitory effect of vitamin C on IKK activity, I-kappaBalpha phosphorylation, and NF-kappaB activation. The results identify p38 as an intracellular target for high dose vitamin C.
2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: Recommendations for training and education to perform clinical flow cytometry (2007)

As clinical flow cytometry practices continue to expand and immunophenotyping for leukemia and lymphoma becomes more widespread, the need for defined guidelines for training of medical professionals is imperative. Standards of expected knowledge and skills are necessary to ensure reliable test results as well as provide direction to those who are considering adding flow cytometry to their clinical laboratory practice. Before now, no clear guidelines have been established for defining the areas of responsibility, education and training standards, and credentials that would be required to perform clinical flow cytometry for leukemia and lymphoma. As part of the 2006 Bethesda Consensus conference, a committee was formed to address this need and provide recommendations for training and education. The committee included laboratory professionals from private, public, and university hospitals as well as large reference laboratories that routinely operate clinical flow cytometry laboratories with an emphasis on lymphoma and leukemia immunophenotyping. This document represents the work of the committee. Categories of work responsibility are defined and the requisite education, training, and credentials, as well as measurement methods for assessing competency for each area of responsibility are provided. Additional recommendations are included that promote creating a specialty certification in flow cytometry, establishing benchmarks for training technologists and interpreters, and offer suggestions for minimum levels of experience to direct a clinical flow cytometry laboratory.
Increasing the Aqueous Solubility of Acetaminophen in the Presence of Polyvinylpyrrolidone and Investigation of the Mechanisms Involved (2003)

It was shown that the aqueous solubility of acetaminophen in the presence of polyvinylpyrrolidone (PVP) increased. The solubility at 25 degrees C increased from 14.3 m mL(-1) in the absence of PVP, to 19.7 mg m(-1) in the presence of 4% w/v PVP, and to 26.7mg mL(-1) in the presence of 8% w/v PVP. Dialysis studies indicated that there is a potential of binding between PVP and acetaminophen in their aqueous solutions. Dialysis studies also revealed that the nature of interaction between PVP and acetaminophen is physical and reversible, and there was no strong binding between PVP and acetaminophen in their solutions. Infrared spectroscopy of acetaminophen/PVP solid dispersion indicated that the mechanism of interaction between PVP and acetaminophen is via hydrogen bonding. Therefore, the increase in solubility of acetaminophen in the presence of PVP is probably attributed to its ability to form a water-soluble complex with PVP.
A cross-media adaptation strategy for multimedia presentations (1999)

Adaptation techniques for multimedia presentations are mainly concerned with switching between different qualities of single media elements to reduce the data volume and by this to adapt to limited presentation resources. This kind of adaptation, however, is limited to an inherent lower bound, i.e., the lowest acceptable technical quality of the respective media type. To overcome this limitation, we propose cross-media adaptation in which the presentation alternatives can be media elements of different media type, even different fragments. Thereby, the alternatives can extremely vary in media type and data volume and this enormously widens the possibilities to efficiently adapt to the current presentation resources. However, the adapted presentation must still convey the same content as the original one, hence, the substitution of media elements and fragments must preserve the presentation semantics. Therefore, our cross-media adaptation strategy provides models for the automatic augmentation of multimedia documents by semantically equivalent presentation alternatives. Additionally, during presentation, substitution models enforce a semantically correct information flow in case of dynamic adaptation to varying presentation resources. The cross-media adaptation strategy allows for flexible reuse of multimedia content in many different environments and, at the same time, maintains a semantically correct information flow of the presentation.
A complex arrangement of genes at a starch branching enzyme I locus in the D-genome donor of wheat (1997)

Genomic DNA fragments from Triticum tauschii (D-genome donor to wheat) carrying starch branching enzyme I (SBE I) type genes have been characterized. One fragment contains one complete gene and two partial genes in 16 kb of DNA. One of the partial genes is oriented in the opposite strand to the other two. The gene that is complete was sequenced. Its structure corresponds closely to that of rice in that exons 3-8 are retained at similar sizes and spacings. A cDNA closely corresponding to the complete gene was isolated and characterized; it codes for a putative protein that represents a novel type of SBE I, as it is shorter at the 3' end than the forms reported so far in other plants. A second genomic fragment contains a different SBE I gene. There appear to be approximately 10 copies of SBE I type genes in wheat (approximately 5 in T. tauschii) and most of them have been assigned to group 7 chromosomes. In situ hybridization indicates that a major locus for the genes is located at the distal end of the short arm of chromosome 7D.
Global optimizations for parallelism and locality on scalable parallel machines (1993)

article Global optimizations for parallelism and locality on scalable parallel machines Share on Authors: Jennifer M. Anderson View Profile , Monica S. Lam View Profile Authors Info & Claims ACM SIGPLAN NoticesVolume 28Issue 6June 1993 pp 112–125https://doi.org/10.1145/173262.155101Published:01 June 1993 268citation690DownloadsMetricsTotal Citations268Total Downloads690Last 12 Months7Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Adenosine mediates hypoxic induction of vascular endothelial growth factor in retinal pericytes and endothelial cells. (1996)

These data suggest that the hypoxia-induced accumulation of adenosine stimulates VEGF gene expression through stimulation of adenosine A2a receptor and subsequent activation of the cAMP-dependent protein kinase A pathway in retinal vascular cells.
Bias dependent dual band response from InAs∕Ga(In)Sb type II strain layer superlattice detectors (2007)

We report on the multispectral properties of infrared photodetectors based on type II InAs∕Ga(In)Sb strain layer superlattices using an nBn heterostructure design. The optical and electrical properties of the midwave and long wave infrared (MWIR-LWIR) absorbing layers are characterized using spectral response and current-voltage measurements, respectively. The dual band response is achieved by changing the polarity of applied bias. The spectral response shows a significant change in the LWIR to MWIR ratio within a very small bias range (∼100mV), making it compatible with commercially available readout integrated circuits.
&lt;title&gt;Analysis of microparticle penetration into human and porcine skin: non-invasive imaging with multiphoton excitation microscopy&lt;/title&gt; (2002)

At the University of Oxford and PowderJect Pharmaceuticals plc, a unique form of needle-free injection technology has been developed. Powdered vaccines and drugs in micro-particle form are accelerated in a high-speed gas flow to sufficient velocity to enter the skin, subsequently achieving a pharmaceutical effect. To optimize the delivery of vaccines and drugs with this method a detailed understanding of the interactive processes that occur between the microparticles and the skin is necessary. Investigations to date of micro-particle delivery into excised human and animal tissue have involved image analyses of histology sections. In the present study, a series of investigations were conducted on excised human and porcine skin using the technique of Multi-Photon fluorescence excitation Microscopy (MPM) to image particles and skin structures post-penetration. Micro-particles of various size and composition were imaged with infrared laser excitation. Three-dimensional images of stratum corneum and epidermal cell deformation due to micro-particle penetration were obtained. Measurements of micro-particle penetration depth taken from z-scan image stacks were used to successfully quantify micro-particle distribution within the skin, without invasively disrupting the skin target. This study has shown that MPM has great potential for the non-invasive imaging of particle skin interactive processes that occur with the transdermal delivery of powdered micro-particle vaccines and drugs.
Trace-Element Analysis in Clinical Chemistry (1971)

Abstract Present knowledge of human bodily contents and concentrations in blood, urine, and hair of 11 essential trace elements and 17-22 nonessential inert or toxic trace elements is reviewed and summarized. Analyses of trace elements are applicable as diagnostic aids and indices for therapy in a number of clinical conditions. Techniques are not difficult, and analyses will probably become more or less routine for many diseases in which primary or secondary abnormalities are manifest. Trace elements play fundamental roles in human metabolism.
Different Inducibility of Expression of the Two Xylanase Genes xyn1 and xyn2 in Trichoderma reesei (1996)

Regulation of formation of the extracellular xylanase system of Trichoderma reesei QM 9414 during growth on xylan, cellulose, and replacement onto a number of soluble inducers was investigated by Northern analysis of xyn1 and xyn2 transcripts and by the use of the Escherichia coli hph (hygromycin B-phosphotransferase-encoding) gene as a reporter. Whereas the xyn1 promoter is active in the presence of xylan and xylose, and virtually silenced in the presence of glucose, the xyn2 promoter enables basal transcription at a low level, but is enhanced in the presence of xylan and xylobiose and also of sophorose or cellobiose. The respective regulatory nucleotide regions were localized on a 221-base pair fragment and a 55-base pair fragment of the xyn1 and xyn2 5'-upstream noncoding sequences, respectively. Electrophoretic mobility shift assays, using cell-free extracts, identified induction-specific protein-DNA complexes: one complex of high mobility was observed under basal, noninduced conditions (glucose) with xyn2, which was in part replaced by a slow-migrating complex upon induction by xylan or sophorose. Both complexes bound to a CCAAT box. With xyn1, the induced complex also binds to a CCAAT box, but this binding is not observed in the presence of the carbon catabolite repressor Cre1, which binds to a nearby located consensus motif.
Detection, identification, and reconstruction of faulty sensors with maximized sensitivity (1999)

Abstract A new method proposed here detects, reconstructs, and identifies faulty sensors using a normal process model, which can be built from first principles or statistical methods such as partial least squares or principal component analysis. The model residual is used to detect sensor faults that demonstrate a deviation from the normal process model. To identify which sensor is faulty, a structured residual approach with maximized sensitivity is proposed to make one residual insensitive to one subset of faults but most sensitive to other faults. The structured residuals are subject to exponentially weighted moving average filtering to reduce the effect of noise and dynamic transients. The confidence limits for these filtered structured residuals are determined using statistical inferential techniques. In addition, other indices including generalized likelihood ratio test, cumulative sum, and cumulative variance of the structured residuals are compared to identify faulty sensors. The fault magnitude is then estimated based on the model and faulty data. Four types of sensor faults, including bias, precision degradation, drifting and complete failure, are simulated to test this method. Data from an industrial boiler process are used to test its effectiveness. Both single faults and simultaneous double faults are detected and uniquely identified with the method.
Rapid Prototyping, Principles and Applications (2010)

Rapid prototyping (RP) has revolutionized how prototypes are made and small batch manufacturing is carried out. With rapid prototyping, the strategies used to produce a part change a number of important considerations and limitations previously faced by tool designers and engineers. Now in its third edition, this textbook is still the definitive text on RP. It covers the key RP processes, the available models and specifications, and their principles, materials, advantages and disadvantages. Examples of application areas in design, planning, manufacturing, biomedical engineering, art and architecture are also given. The book includes several related problems so that the reader can test his or her understanding of the topics. New to this edition, the included DVD-ROM presents animated illustrations of the working principles of today's key RP processes.
Broken Scan Chain Diagnostics Based on Time-Integrated and Time-Dependent Emission Measurements (2004)

Abstract Light Emission due to Off-State Leakage Current (LEOSLC) is used in combination with the Picosecond Imaging Circuit Analysis (PICA) method to effectively diagnose and localize defects in a broken scan chain. As usual, the emission base method shows to be very effective in debugging the problem; the defect is successfully identified by the optical technique and confirmed by Physical Failure Analysis (PFA).
Type checking modular multiple dispatch with parametric polymorphism and multiple inheritance (2011)

In previous work, we presented rules for defining overloaded functions that ensure type safety under symmetric multiple dispatch in an object-oriented language with multiple inheritance, and we showed how to check these rules without requiring the entire type hierarchy to be known, thus supporting modularity and extensibility. In this work, we extend these rules to a language that supports parametric polymorphism on both classes and functions.
Long-term weight control study: Conclusions (1992)

Clinical Pharmacology and Therapeutics (1992) 51, 642–646; doi:10.1038/clpt.1992.76
Wavelength- and polarization-independent large angle InP/lnGaAsP digital optical switches with extinction ratios exceeding 20 dB (1994)

Record polarization-independent extinction ratios are reported for ridge waveguide digital optical switches in InP/InGaAsP. Wavelength insensitivity is demonstrated for the first time, with extinction ratios that exceed 20 dB at both 1.3 and 1.5 μm for the same switch. The switch's total opening angle of 0.5/spl deg/ is more than a factor of two larger than other digital optical switches demonstrated at any wavelength.