gebhart/patrepeval huggingface dataset:
A rapid separation of S100 subunits by high performance liquid chromatography: the subunit compositions of S100 proteins. (1983)

The subunits of S100 protein were dissociated and separated from each other by high performance liquid chromatography on a macroreticular poly-styrene resin, using an acetonitrile-trifluoroacetic acid solvent system. The separation was completed within 80 min in monitoring the effluent at 210 and 280 nm, allowing a rapid and sensitive identification of each S100 protein in terms of subunit composition, and a simultaneous purification of subunits. The method enabled to estimate the subunit compositions of S100a, S100b, and S100a0 protein purified from bovine brain, and revealed a micro-heterogeneity of S100 subunits as applied to the mixtures of S100 protein prepared from bovine, human and rat brain.
Spectrochemical Analysis of Liquids Using Laser-Induced Plasma Emissions: Effects of Laser Wavelength (1997)

The plasma plume emissions produced by pulsed (∼ 10 ns) laser ablation of liquid jets were monitored for spectrochemical analysis. Laser wavelengths at 532 and 193 nm were used, and sodium was the test analyte. As expected, the 532-nm laser pulse produced very intense plasma continuum emissions that masked the sodium signal for the first hundred nanoseconds, especially near the bright core of the vapor plume. Neither time-gating nor spatial masking could significantly improve the single-shot signal-to-noise ratio, since the transient nature of the emissions placed stringent demands on timing precision while the small size of the plume required accurate mask positioning—both antithetical to the inherent instability of jet ablation. In sharp contrast, the 193-nm laser pulse produced relatively dim plasma flash but intense sodium emissions, rendering it ideal for analytical applications.
Short-term evaluation of periradicular corticosteroid injections in the treatment of lumbar radiculopathy associated with disc disease. (1998)

Periradicular injections of corticosteroids done under fluoroscopic guidance as an outpatient procedure was effective and safe in our study and may deserve to be used as part of the conservative management of lumbar nerve root pain before resorting to more invasive methods.
Superconductivity at High Magnetic Fields and Current Densities in Some Nb-Zr Alloys (1961)

The results on Nb-Zr alloys were obtained during investigation of resistive superconducting transitions in certain Ti-, Zr-, Nb-, Hf-, and Ta-rich lunary alloys in magnetic fields up to 30 kgauss. The data show that cold-worked Nbrich Nb-Zr alloys display zero electrical resistance at current densities as high as 10/sup 5/ amp/cm/sup 2/ at 30 kgauss and at 4.2 deg K. Present measurements reveal high-field superconducting effects in cold-rolled Nb-Zr alloys such as a marked dependence of the magnitude of the maximum zeroresistance current upon the direction of the externally applied transverse magnetic-field rehative to the rolled surface of the specimen, and anomalous increase of the maximum zero-resistance current with increase of magnetic field strength over certain regions in certain alloy specimens. The source materials, method of fabrication, and cross-sectional dimensions of the specimens are given. (P-C-H- )
The recombinant immunotoxin anti-Tac(Fv)-Pseudomonas exotoxin 40 is cytotoxic toward peripheral blood malignant cells from patients with adult T-cell leukemia. (1990)

Anti-Tac(Fv)-PE40 is a recombinant single-chain immunotoxin containing the heavy and light variable regions of the anti-Tac monoclonal antibody fused to a mutant form of Pseudomonas exotoxin (PE). Anti-Tac binds to the p55 subunit of the human interleukin 2 (IL-2) receptor, and anti-Tac(Fv)-PE40 kills human or monkey cell lines that contain either the intact IL-2 receptor or its p55 subunit alone. To assess the usefulness of anti-Tac(Fv)-PE40 in treatment of IL-2 receptor-positive leukemia, we tested peripheral blood mononuclear cells from six patients with adult T-cell leukemia. In each of the six patients, anti-Tac(Fv)-PE40 was extremely cytotoxic to the malignant cells. Metabolic activity and sensitivity of the fresh cells improved when a small amount of IL-2 (10 units per ml) was present during incubation. The toxin concentration necessary to inhibit protein synthesis by 50% after 16-hr incubation of cells with immunotoxin varied from 1.6 to 16 ng/ml (2.5-25 x 10(-11) M). In every case, binding was by means of the Tac antigen because anti-Tac(Fv)-PE40 cytotoxicity was prevented by adding excess anti-Tac antibody. Moreover, anti-Tac alone or an inactive mutant of anti-Tac(Fv)-PE40 without ADP-ribosylation activity had very little cytotoxic activity. Peripheral blood mononuclear cells from normal controls, from a patient with Tac-negative leukemia, and from adult T-cell leukemia patients without significant peripheral blood involvement were not sensitive to anti-Tac(Fv)-PE40. These results indicate that anti-Tac(Fv)-PE40 is a potent cytotoxin against adult T-cell leukemia cells in vitro and warrants clinical testing.
Stereochemical Effects in Mass Spectrometry - Determination of the Optical Purity of Enantiomers by Mass Spectrometry (1997)

Abstract Due to the difference in reactivity of enantiomers with chiral reagents, it was found that in the Fast Atom Bombardment (FAB) mass spectra of enantiomer mixtures of R-α-phenylethylamine and S-α-phenylethylamine with a chiral reagent S-phosphorsaeure-(1,1′-bi-naphthyl-2,2′-diylester) (BNP acid), the relative abundance of the characteristic ion is linearly related to the composition of the mixture. We have, therefore, proposed a possible method for the determination of the optical purity of enantiomers by mass spectrometry.
천안함 이후 북중관계의 변화와 영향 (2011)

This thesis evaluated and analyzed the relation between North Korea and China through China`s North Korea policy development in the perspective of continuation and change after the Cheonan sinking. As a result of the analysis, North Korea-China relation shows not only the continuation of the previous identical pattern before the Cheonan incident but also the change in the pattern. In other words, the Cheonan sinking operates as an impetus that enhances North Korea-China`s overall relation by converging strategic advantages of both China and North Korea. And should this kind of composition be sustained, consolidation of North Korea-China relation would be inevitable. Influences from the consolidation of North Korea-China relation are complex. That is. the reinforcement of the relation contributes to North Korea`s stability and survival, but poses limitation on the change of North Korea, such as its reform and opening up. Also, it would serve to maintain the motive of the six-party talk, it, however, implies the side of postponing the resolution of North Korea`s nuclear problem. Furthermore, the reinforced relation would benefit the relation between South Korea and North Korea while restraining the overall improvement. Thus, it is necessary for us to alleviate disadvantageous influences from the consolidation of North Korea and China relation and induce them to benefit us. In order for that to happen, we would need to concentrate on the positive aspects that the reinforcement of the two nations` relation have on us. The reinforcement of North Korea-China relation, for instance, would set a turning point and provide opportunities for the resolution of North Korea`s nuclear issue, open-assistance to North Korea and the relaxation of Korean peninsula and South-North Korea relation by restarting Six-Party talks.
Methicillin-resistant <i>Staphylococcus aureus</i> infection in combat support hospitals in three regions of Iraq (2010)

SUMMARYStaphylococcus aureus is a leading cause of infections in deployed service members. Based on a molecular epidemiological study of 182 MRSA isolates from patients in three U.S. Army combat support hospitals in separate regions in Iraq, USA300 clone was the most predominant (80%) pulsotype. This finding suggested that strain carriage from the home country by military personnel is epidemiologically more important than local acquisition.
Tracing evolution changes of software artifacts through model synchronization (2004)

Software evolution encompasses all activities related to engineering software, from its inception to retirement. Propagating change across software models that are altered due to maintenance activities is a first step towards maintaining consistency between architectural design, and implementation models. Model synchronization techniques initially presented within the context of model driven architecture provide an instrument for achieving change trace-ability and consistency. We present a framework whereby software artifacts at different levels of abstraction such as architecture diagrams, object models, and abstract syntax trees are represented by graph-based MOF compliant models that can be synchronized using model transformations. In such a framework model dependencies are implicitly encoded using transformation rules and an equivalence relation is used to evaluate when two models become synchronized.
Conditionally Replicating Adenovirus Expressing TIMP2 for Ovarian Cancer Therapy (2010)

Results support the translational potential of Ad5/3-CXCR4-TIMP2 for treatment of patients with advanced ovarian cancer.
Using blockchain to build trusted LoRaWAN sharing server (2017)

Purpose With the rapid growth of the Internet of Things (IoT) market and requirement, low power wide area (LPWA) technologies have become popular. In various LPWA technologies, Narrow Band IoT (NB-IoT) and long range (LoRa) are two main leading competitive technologies. Compared with NB-IoT networks, which are mainly built and managed by mobile network operators, LoRa wide area networks (LoRaWAN) are mainly operated by private companies or organizations, which suggests two issues: trust of the private network operators and lack of network coverage. This study aims to propose a conceptual architecture design of a blockchain built-in solution for LoRaWAN network servers to solve these two issues for LoRaWAN IoT solution. Design/methodology/approach The study proposed modeling, model analysis and architecture design. Findings The proposed solution uses the blockchain technology to build an open, trusted, decentralized and tamper-proof system, which provides the indisputable mechanism to verify that the data of a transaction has existed at a specific time in the network. Originality/value To the best of our knowledge, this is the first work that integrates blockchain technology and LoRaWAN IoT technology.
Topological structure and chemical composition of inner surfaces of borosilicate vials. (2004)

The use of atomic force microscopy (AFM) and x-ray photoelectron spectroscopy (XPS) is described to characterize the inner surfaces of pharmaceutical vials. The two type I borosilicate glasses included in this study slightly differ in their amounts of alkaline oxides. The topography and chemistry of the inner surfaces of vials are predominantly caused by the forming process. A structural and chemical modification of the inner surface of vials was also observed when exposing the surface to different pH conditions and special treatment like washing and sterilization, which are routine operation steps during galenical manufacturing.
Na<sup>+</sup>/H<sup>+</sup> antiport activity in tonoplast vesicles from roots of the salt‐tolerant <i>Plantago maritima</i> and the salt‐sensitive <i>Plantago media</i> (1991)

Plantago species differ in their strategy towards salt stress, a major difference being the uptake and distribution of Na + ions. A salt‐sensitive ( Plantago media L.) and a salt‐tolerant ( P. maritima L.) species were compared with respect to Na + /H + antiport activities at the tonoplast. After exposure of the plants to 50 m M NaCl for 6 days isolated tonoplast vesicles of P. maritima showed Na + /H + antiport activity with saturation kinetics and a K m of 2.4 m M Na + , NaCl‐grown P. media and the control plants of both species showed no antiport activity. Selectivity of the antiport system for Na + was high and was determined by adding different chloride salts after formation of a Δ pH in the vesicles. Specific tonoplast ATPase activities were similar in the two species and did not alter after exposure to NaCl stress.
3D touchable holographic light-field display (2016)

We propose a new type of 3D user interface: interaction with a light field reproduced by a 3D display. The 3D display used in this work reproduces a 3D light field, and a real image can be reproduced in midair between the display and the user. When using a finger to touch the real image, the light field from the display will scatter. Then, the 3D touch sensing is realized by detecting the scattered light by a color camera. In the experiment, the light-field display is constructed with a holographic screen and a projector; thus, a preliminary implementation of a 3D touch is demonstrated.
Novel Antibody-Based Therapies For Acute Lymphoblastic Leukemia (2011)

A major breakthrough in the treatment of acute lymphoblastic leukemia (ALL) was the availability of targeted therapies targeting either specific transcripts, such as bcr-abl fusion protein by tyrosine kinase inhibitors (TKIs), or specific antigens by mAbs. ALL blast cells express a variety of specific antigens (eg, CD19, CD20, CD22, CD33, and CD52) that serve as targets for mAbs. To date, the most data are available for anti-CD20 (rituximab), which has been combined with chemotherapy for the treatment of mature B-ALL/Burkitt lymphoma. Studies with rituximab have also been completed in B-precursor ALL. Another antigen, CD19, is of great interest due to a very high rate of expression in ALL. It can be targeted by a bispecific mAb, blinatumomab, directed against CD19 and CD3. Smaller studies or case reports are also available for the anti-CD52 (alemtuzumab), anti-CD22 (epratuzumab), and anti-CD33 (gemtuzumab) mAbs. Available data demonstrate that mAb therapy in ALL is a highly promising treatment approach. However, several details for an optimal treatment approach, such as the required level of antigen expression, timing, schedule, dosage, and stage of disease, still need to be defined.
Recombinant protein expression in Escherichia coli: advances and challenges (2014)

Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Analyses of Nursing Home Residents with Human Immunodeficiency Virus and Depression Using the Minimum Data Set (2002)

This study compares nursing home residents with human immunodeficiency virus (HIV) who have a diagnosis of depression to other nursing home residents with HIV. We analyzed 1057 HIV residents with a diagnosis of depression and 4057 other residents with HIV using admission assessments recorded in the minimum data set between June 22, 1998 and January 17, 2000. Residents with HIV and depression were significantly more likely to be older, female, and white than other residents with HIV. Residents with HIV and depression were twice as likely as other residents with HIV to have a history of mental health conditions and were significantly more likely to have unsettled relationships. However, there were no significant differences between these two groups of residents with HIV in levels of physical disability or in cognitive ability. Residents with HIV and depression were significantly more likely to have unstable health conditions, to be at the end stage of disease, and to have other diseases and infections than other residents with HIV. One third of residents with HIV and depression did not receive antidepressants and most had not been evaluated by a mental health specialist in the last 90 days or received psychological therapy in the previous 7 days. Many residents with HIV and depression are not receiving antidepressants or mental health services in nursing homes. Additional research is needed to determine how the mental health needs of residents with HIV change as their stay in the nursing home increases and how mental health therapies affect their health.
Asymmetric partitioning with non-power-of-two transform for intra coding (2012)

HEVC (High Efficiency Video Coding) is an ongoing standardization target as the next generation of video compression technology. HEVC employs a coding tree block, which is a quad-tree structure of a coding unit. It also employs some unit types; coding unit, prediction unit, and transform unit. A coding unit can be divided into smaller units as prediction units. Though an asymmetric unit is used for inter coding, only symmetric units are permitted for intra coding. In this paper, we propose an asymmetric partitioning with a non-power-of-two transform as a prediction and transform unit. While conventional partitioning locates the cross-point of partitioning lines at the center of the coding unit, the proposed method locates the cross-point in places except center. The proposed method reduces 2.0% BD-bitrate compared with HM5.0 under all intra / high efficiency condition. The validity of the proposed method is confirmed by some experimental results.
Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species (1992)

A comparison was made of the antigenic composition of oocyst walls and sporozoites from Cryptosporidium baileyi from turkeys, C. muris from rodents, and C. parvum from ruminants, employing immunoblotting and immunofluorescence. In immunoblotting, oocyst antigens were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting) and detected with rabbit polyclonal anti-C. muris or -C. parvum antibodies or murine monoclonal antibodies developed against C. parvum. Immunofluorescence was used to investigate the reactivity of these monoclonal antibodies with air-dried excystation mixtures of sporozoites and oocysts of the different species. The results from both types of experiment indicated that the three Cryptosporidium species could be differentiated immunologically. In comparison, few antigenic differences were found between a number of isolates of C. parvum in immunoblotting. There was also evidence to suggest that C. parvum and C. baileyi were more closely related antigenically to one another than to C. muris.
Intralesional Injection of OK-432 in Cystic Hygroma. (2016)

Intralesional OK-432 injection is an effective and safe alternative in treating cystic hygroma.
Inhibitors of Bacillus subtilis DNA polymerase III. 6-Anilinouracils and 6-(alkylamino)uracils (1980)

Substituted 6-anilinouracils were found to be potent inhibitors of the replication-specific enzyme, DNA polymerase III, from Bacillus subtilis. Inhibition potency was maximized by inclusion of small alkyl groups or halogens in the meta and para positions of the phenyl ring; polar substituents decreased activity considerably. Qualitative structure--activity relationships indicated that the meta position can tolerate larger groups, suggesting that this position may be suitable for the introduction of a group capable of irreversibly binding to the enzyme. Several 6-(alkylamino)uracils were weak inhibitors of DNA polymerases III; the optimum alkyl groups for enzyme binding were n-pentyl and n-hexyl, which apparently can occupy the planar enzyme binding site. The varied activities of 6-anilinouracils on a mutant DNA polymerase, resistant to 6-(phenylhydrazino)- and 6-(benzylamino)uracils bearing a p-OH or NH2 group, have altered previous postulates for the structural basis of inhibitor resistance and have permitted construction of a refined model for inhibitor conformation in the latter series.
Total synthesis of prodigiosin, prodigiosene, and desmethoxyprodigiosin: Diels-Alder reactions of heterocyclic azadienes and development of an effective palladium(II)-promoted 2,2'-bipyrrole coupling procedure (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of prodigiosin, prodigiosene, and desmethoxyprodigiosin: Diels-Alder reactions of heterocyclic azadienes and development of an effective palladium(II)-promoted 2,2'-bipyrrole coupling procedureDale L. Boger and Mona PatelCite this: J. Org. Chem. 1988, 53, 7, 1405–1415Publication Date (Print):April 1, 1988Publication History Published online1 May 2002Published inissue 1 April 1988https://doi.org/10.1021/jo00242a013Request reuse permissionsArticle Views2479Altmetric-Citations174LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Intramolecular Dehydrogenative Coupling of sp<sup>2</sup> C–H and sp<sup>3</sup> C–H Bonds: An Expeditious Route to 2-Oxindoles (2012)

An intramolecular-dehydrogenative-coupling (IDC) using "transition-metal-free" oxidation conditions has been achieved to synthesize a variety of 2-oxindoles bearing an all-carbon quaternary stereogenic center at the benzylic position. The methodology involves a one-pot C-alkylation of β-N-arylamido esters (3, 6) with alkyl halides using potassium tert-butoxide concomitant with a dehydrogenative coupling. A radical-mediated pathway has been tentatively proposed for the oxidative process.
Detection and Characterization of UDP-GalNAc: Chondroitin N-Acetylgalactosaminyltransferase in Bovine Serum Using a Simple Assay Method1 (1995)

We report the occurrence, in bovine serum of a soluble form, of N-acetylgalactosaminyl-transferase activity, which is involved in chondroitin sulfate biosynthesis, and we describe a simple assay method for the enzyme. Chondroitin with the general structure [GlcA-GalNAc], was found to serve as acceptor substrates for the enzyme. To develop a routine assay, bovine serum was incubated with polymeric chondroitin and UDP-[3H]GalNAc. After the removal of labeled endogenous acceptors derived from serum by trichloroacetic acid treatment, the labeled chondroitin sulfate chains were separated from residual unincorporated label by centrifugation using syringe columns packed with Sephadex G-25, and the effluent fractions were monitored by scintillation counting. This simple method allowed us to perform multiple assays and to establish assay conditions for the serum N-acetylgalactosaminyltransferase involved in chondroitin polymerization.