gebhart/patrepeval huggingface dataset:
Metabolic profiling reveals a contribution of gut microbiota to fatty liver phenotype in insulin-resistant mice (2006)

Here, we study the intricate relationship between gut microbiota and host cometabolic phenotypes associated with dietary-induced impaired glucose homeostasis and nonalcoholic fatty liver disease (NAFLD) in a mouse strain (129S6) known to be susceptible to these disease traits, using plasma and urine metabotyping, achieved by (1)H NMR spectroscopy. Multivariate statistical modeling of the spectra shows that the genetic predisposition of the 129S6 mouse to impaired glucose homeostasis and NAFLD is associated with disruptions of choline metabolism, i.e., low circulating levels of plasma phosphatidylcholine and high urinary excretion of methylamines (dimethylamine, trimethylamine, and trimethylamine-N-oxide), coprocessed by symbiotic gut microbiota and mammalian enzyme systems. Conversion of choline into methylamines by microbiota in strain 129S6 on a high-fat diet reduces the bioavailability of choline and mimics the effect of choline-deficient diets, causing NAFLD. These data also indicate that gut microbiota may play an active role in the development of insulin resistance.
Relief of Angina Pectoris by Electrical Stimulation of the Carotid-Sinus Nerves (1967)

BECAUSE angina pectoris is a consequence of inadequate myocardial oxygenation, ideal therapy for this incapacitating symptom would be directed toward both increasing coronary blood flow and decreasing myocardial oxygen requirements. In the setting of severe coronary-artery disease, however, the ability of the coronary vascular bed to dilate is limited so that methods designed to decrease myocardial oxygen requirements may provide a more fruitful approach. Recent experimental studies have demonstrated that these oxygen requirements are directly related to the heart rate, to the inotropic state of the myocardium and to the intraventricular pressure, which is a reflection of the intramyocardial wall . . .
Inhibition of vesicular stomatitis virus protein synthesis and infection by sequence-specific oligodeoxyribonucleoside methylphosphonates (1986)

Oligodeoxyribonucleoside methylphosphonates which have sequences complementary to the initiation codon regions of N, NS, and G vesicular stomatitis virus (VSV) mRNAs were tested for their ability to inhibit translation of VSV mRNA in a cell-free system and in VSV-infected mouse L cells. In a rabbit reticulocyte lysate cell-free system, the oligomers complementary to N (oligomer I) and NS (oligomer II) mRNAs inhibited translation of VSV N and NS mRNAs whereas oligomer III had only a slight inhibitory effect on N protein synthesis. At 100 and 150 microM, oligomer I specifically inhibited N protein synthesis in the lysate. In contrast, at 150 microM, oligomer II inhibited both N and NS protein synthesis. This reduced specificity of inhibition may be due to the formation of partial duplexes between oligomer II and VSV N mRNA. The oligomers had little or no inhibitory effects on the synthesis of globin mRNA in the same lysate system. Oligomers I-III specifically inhibited the synthesis of all five viral proteins in VSV-infected cells in a concentration-dependent manner. The oligomers had no effects on cellular protein synthesis in uninfected cells nor on cell growth. An oligothymidylate which forms only weak duplexes with poly(rA) had just a slight effect on VSV protein synthesis and yield of virus. Oligomers I-III have extensive partial complementarity with the coding regions of L mRNA. The nonspecific inhibition of viral protein synthesis in infected cells may reflect the role of N, NS, and/or L proteins in the replication and transcription of viral RNA or result from duplex formation between the oligomers and complementary, plus-strand viral RNA.(ABSTRACT TRUNCATED AT 250 WORDS)
Size and Support Effects for the Water–Gas Shift Catalysis over Gold Nanoparticles Supported on Model Al<sub>2</sub>O<sub>3</sub> and TiO<sub>2</sub> (2012)

The water-gas shift (WGS) reaction rate per total mole of Au under 7% CO, 8.5% CO(2), 22% H(2)O, and 37% H(2) at 1 atm for Au/Al(2)O(3) catalysts at 180 °C and Au/TiO(2) catalysts at 120 °C varies with the number average Au particle size (d) as d(-2.2±0.2) and d(-2.7±0.1), respectively. The use of nonporous and crystalline, model Al(2)O(3) and TiO(2) supports allowed the imaging of the active catalyst and enabled a precise determination of the Au particle size distribution and particle shape using transmission electron microscopy (TEM). Further, the apparent reaction orders and the stretching frequency of CO adsorbed on Au(0) (near 2100 cm(-1)) determined by diffuse reflectance infrared spectroscopy (DRIFTS) depend on d. Because of the changes in reaction rates, kinetics, and the CO stretching frequency with number average Au particle size, it is determined that the dominant active sites are the low coordinated corner Au sites, which are 3 and 7 times more active than the perimeter Au sites for Au/Al(2)O(3) and Au/TiO(2) catalysts, respectively, and 10 times more active for Au on TiO(2) versus Al(2)O(3). From operando Fourier transform infrared spectroscopy (FTIR) experiments, it is determined that the active Au sites are metallic in nature. In addition, Au/Al(2)O(3) catalysts have a higher apparent H(2)O order (0.63) and lower apparent activation energy (9 kJ mol(-1)) than Au/TiO(2) catalysts with apparent H(2)O order of -0.42 to -0.21 and activation energy of 45-60 kJ mol(-1) at near 120 °C. From these data, we conclude that the support directly participates by activating H(2)O molecules.
An Airline Seat Management Model for a Single Leg Route When Lower Fare Classes Book First (1992)

It is common practice for airlines to charge several different fares for a common pool of seats. This paper presents a model that has been used to address the problem of when to refuse booking requests for a given fare level to save the seat for a potential request at a higher fare level. This model assumes that lower fare passengers book before higher fare passengers book. This occurs quite frequently in practice, where lower fare passengers are usually vacationers and higher fare passengers are usually business travelers. In fact, many airlines mandate this practice by imposing advance booking requirements on lower fare classes to prevent business travelers from using them. We present an algorithm that finds a seat management policy that maximizes mean revenue by establishing a critical value for each fare class. Booking requests for a particular fare level are accepted if the number of empty seats is strictly greater than its critical value and rejected otherwise. This critical value is a decreasing function of the fare price and is equal to zero for the class with the highest fare.
Tuning Carbon Nanotube Band Gaps with Strain (2003)

We show that the band structure of a carbon nanotube (NT) can be dramatically altered by mechanical strain. We employ an atomic force microscope tip to simultaneously vary the NT strain and to electrostatically gate the tube. We show that strain can open a band gap in a metallic NT and modify the band gap in a semiconducting NT. Theoretical work predicts that band gap changes can range between +/-100 meV per 1% stretch, depending on NT chirality, and our measurements are consistent with this predicted range.
Search-based software test data generation: a survey: Research Articles (2004)

The use of metaheuristic search techniques for the automatic generation of test data has been a burgeoning interest for many researchers in recent years. Previous attempts to automate the test generation process have been limited, having been constrained by the size and complexity of software, and the basic fact that, in general, test data generation is an undecidable problem. Metaheuristic search techniques offer much promise in regard to these problems. Metaheuristic search techniques are high-level frameworks, which utilize heuristics to seek solutions for combinatorial problems at a reasonable computational cost. To date, metaheuristic search techniques have been applied to automate test data generation for structural and functional testing; the testing of grey-box properties, for example safety constraints; and also non-functional properties, such as worst-case execution time. This paper surveys some of the work undertaken in this field, discussing possible new future directions of research for each of its different individual areas. Copyright © 2004 John Wiley & Sons, Ltd.
Localization of monocyte chemoattractant peptide-1 expression in the central nervous system in experimental autoimmune encephalomyelitis and trauma in the rat (1996)

Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine beta family of chemoattractants that has been shown to play a major role in the initiation of monocyte and T cell inflammation to sites of tissue injury. In this study, we have examined the distribution of MCP-1 expression in inflammation in the central nervous system (CNS) associated with the autoimmune disease experimental autoimmune encephalomyelitis (EAE) and compared the results with those detected in inflammation associated with trauma. In EAE, MCP-1 expression was detected at the onset of inflammation, prior to clinical expression of disease, in lymphocytes and endothelial cells in subarachnoid locations. Monocyte infiltration into these areas appeared 24 h later. After the onset of clinical signs, MCP-1 expression was widely distributed in the spinal cord with levels increasing and decreasing in association with disease activity. Lymphocytes, macrophages, astrocytes, and endothelial cells could be identified as sources of MCP-1 by immunoreactivity and in situ hybridization. A similar close correlation between macrophage infiltration and the levels of mRNA for MCP-1 was found in the CNS of rats subjected to trauma, and in these animals MCP-1 was detected by immunohistochemistry in macrophages and endothelial cells. The results support the conclusion that MCP-1 is an important mediator of inflammation in the CNS.
Identification of Motility and Autoagglutination <i>Campylobacter jejuni</i> Mutants by Random Transposon Mutagenesis (2002)

Campylobacter jejuni has been identified as the leading cause of acute bacterial diarrhea in the United States, yet compared with other enteric pathogens, considerably less is understood concerning the virulence factors of this human pathogen. A random in vivo transposon mutagenesis system was recently developed for the purpose of creating a library of C. jejuni transformants. A total of 1,065 C. jejuni transposon mutants were screened for their ability to swarm on motility agar plates and autoagglutinate in liquid cultures; 28 mutants were subsequently identified. The transposon insertion sites were obtained by using random-primed PCR, and the putative genes responsible for these phenotypes were identified. Of these mutants, all 28 were found to have diminished motility (0 to 86% that of the control). Seventeen motility mutants had insertions in genes with strong homology to functionally known motility and chemotaxis genes; however, 11 insertions were in genes of unknown function. Twenty motility mutants were unable to autoagglutinate, suggesting that the expression of flagella is correlated with autoagglutination (AAG). However, four mutants expressed wild-type levels of surface FlaA, as indicated by Western blot analysis, yet were unable to autoagglutinate (Cj1318, Cj1333, Cj1340c, and Cj1062). These results suggest that FlaA is necessary but not sufficient to mediate the AAG phenotype. Furthermore, two of the four AAG mutants (Cj1333 and Cj1062) were unable to invade INT-407 intestinal epithelial cells, as determined by a gentamicin treatment assay. These data identify novel genes important for motility, chemotaxis, and AAG and demonstrate their potential role in virulence.
Effect of BSA on carbon nanotube dispersion for<i>in vivo</i>and<i>in vitro</i>studies (2007)

Carbon nanotubes (CNT) are one of the most promising nanomaterials because of their intrinsic properties. So, it becomes urgent to assess their toxicity. However, CNT are insoluble in aqueous media required for toxicological studies. Thus, we propose a simple method to disperse CNT for toxicological studies using a biomolecule: The albumin. To evaluate this method, several nanotubes were suspended in saline solution (NaCl 0.9%) without or with albumin at a concentration of 0.5 mg/ml or equal as CNT concentration. These suspensions were visually compared to suspensions obtained with classical dispersing methods using Tween 80 or serum. Homogeneity of the suspensions with or without BSA and CNT structure were analyzed by TEM, agglomerates quantification and total carbon dosage. The effect of coupled albumin-CNT was then tested on A549 and U937 cells in vitro and on rats in vivo. Total carbon dosage, agglomerates quantification and TEM revealed that, in the presence of albumin, the tested nanotubes were better dispersed without any modification of their structure. The CNT suspension was tested in vitro and in vivo in rats. Albumin solution alone induced no modification of the biological responses studied (i.e., cell viability in vitro and inflammatory response and histopathology in vivo) compared to the saline. CNT in NaCl or BSA altered cellular viability in vitro in a similar way but results obtained with CNT suspension in the presence of albumin showed a better reproducibility that can be explained by the better homogeneity of the suspensions. CNT in BSA but not in NaCl significantly increased the cell number in BAL and also the number of apparent CNT-containing cells. Taken together, these results highlight the potential importance of CNT dispersion (and thus of the vehicle) for the toxicological studies.
Molecular physiology of the islet amyloid polypeptide (IAPP)/amylin gene in man, rat, and transgenic mice (1994)

Islet amyloid polypeptide ("amylin") is the major protein component of amyloid deposits in pancreatic islets of type 2 (non-insulin-dependent) diabetic patients. Islet amyloid polypeptide consists of 37 amino acids, is co-produced and co-secreted with insulin from islet beta-cells, can act as a hormone in regulation of carbohydrate metabolism, and is implicated in the pathogenesis of islet amyloid formation and of type 2 diabetes mellitus. Rat islet amyloid polypeptide differs from human islet amyloid polypeptide particularly in the region of amino acids 25-28, which is important for amyloid fibril formation. In rat and mouse, diabetes-associated islet amyloid does not develop. To study the genetic organization and biosynthesis of islet amyloid polypeptide, we have isolated and analyzed the human and rat islet amyloid polypeptide gene and corresponding cDNAs. Both genes contain 3 exons, encoding precursor proteins of 89 amino acids and 93 amino acids, respectively. Apart from a putative signal sequence, these precursors contain amino- and carboxy-terminal flanking peptides in addition to the mature islet amyloid polypeptide. To understand regulation of islet amyloid polypeptide gene expression, we have identified several potential cis-acting transcriptional control elements that influence beta-cell-specific islet amyloid polypeptide gene expression. Using antisera raised against synthetic human islet amyloid polypeptide we developed a specific and sensitive radioimmunoassay to measure levels of islet amyloid polypeptide in plasma and tissue extracts. Also antisera raised against the flanking peptides will be used in studying human islet amyloid polypeptide biosynthesis. Elevated plasma islet amyloid polypeptide levels have been demonstrated in some diabetic, glucose-intolerant, and obese individuals, as well as in rodent models of diabetes and obesity. To examine the potential role of islet amyloid polypeptide overproduction in the pathogenesis of islet amyloid formation and type 2 diabetes, we generated transgenic mice that overproduce either the amyloidogenic human islet amyloid polypeptide or the nonamyloidogenic rat islet amyloid polypeptide in their islet beta-cells. Despite moderately to highly (up to 15-fold) elevated plasma islet amyloid polypeptide levels, no marked hyperglycemia, hyperinsulinemia or obesity was observed. This suggests that chronic overproduction of islet amyloid polypeptide "per se" does not cause insulin resistance. No islet amyloid deposits were detected in mice up to 63 weeks of age, but in every mouse producing human islet amyloid polypeptide (as in man), accumulation of islet amyloid polypeptide was observed in beta-cell lysosomal bodies. This may represent an initial phase in intracellular amyloid fibril formation.(ABSTRACT TRUNCATED AT 400 WORDS)
Crosshole GPR full-waveform inversion of waveguides acting as preferential flow paths within aquifer systems (2012)

ABSTRACT High-contrast layers caused by porosity or clay content changes can have a dominant effect on hydraulic processes within an aquifer. These layers can act as low-velocity waveguides for GPR waves. We used a field example from a hydrological test site in Switzerland to show that full-waveform inversion of crosshole GPR signals could image a subwavelength thickness low-velocity waveguiding layer. We exploited the full information content of the data, whereas ray-based inversion techniques are not able to image such thin waveguide layers because they only exploit the first-arrival times and first-cycle amplitudes. This low-velocity waveguide layer is caused by an increase in porosity and indicates a preferential flow path within the aquifer. The waveguide trapping causes anomalously high amplitudes and elongated wavetrains to be observed for a transmitter within the waveguide and receivers straddling the waveguide depth range. The excellent fit of amplitudes and phase between the measured and modeled data confirms its presence. This new method enables detailed aquifer characterization to accurately predict transport and flow and can be applied to a wide range of geologic, hydrological, and engineering investigations.
The complex interplay of palm oil fatty acids on blood lipids (2007)

Abstract The cholesterol‐raising potential of saturated fats has been noted for 50 years, but how they function and which among the saturated fats are most to blame is only now becoming apparent. The focus has shifted from saturated fats to individual fatty acids and the total composite of fatty acids (SFA, MUFA, PUFA) that comprise our daily fat intake. An adequate intake of both PUFA and SFA may be needed for the ideal LDL/HDL ratio in blood, as both contribute to the regulatory balance in lipoprotein metabolism. Palm oil, by virtue of its fatty acid composition equally balanced between saturated and unsaturated fatty acids, impacts blood lipids in different ways, depending on an individual's lipid profile. Recently, the realization that partially hydrogenated fats (containing trans fatty acids) have adverse health effects has necessitated finding alternative fat sources that provide specific functional characteristics without compromising health. The immediate solution would seem to be blending appropriate natural saturated and polyunsaturated fats to meet the combined requirements for food technology and energy metabolism. In technical situations where a higher‐melting point fat is needed, as in margarines, shortenings, and frying oil, palm oil – or more specifically palm olein – would appear to be the fat of choice for blending with unsaturated oils.
Architecture, memory and interface technology integration of an industrial/ academic configurable system-on-chip (CSoC) (2003)

This paper describes the actual status and results of a dynamically configurable system-on-chip (CSoC) integration, consisting of a SPAR C-compatible LEON processor-core, a commercial coarse-grain XPP-array of suitable size from PACT XPP Technologies AG, and application-tailored global/local memory topology with efficient Amba-based communication interfaces. The given adaptive architecture is synthesized within an industrial/academic SoC project onto 0.18 and 0.13 /spl mu/m UMC CMOS technologies at Universitaet Karlsruhe (TH). Due to exponentially increasing CMOS mask costs, essential aspects for the industry are now adaptivity of SoCs, which can be realized by integrating reconfigurable re-usable hardware parts on different granularities into configurable systems-on-chip (CSoCs).
Enhanced antiproliferative and apoptotic response to combined treatment of γ-tocotrienol with erlotinib or gefitinib in mammary tumor cells (2010)

Combination treatment of gamma-tocotrienol with specific ErbB receptor inhibitors is more effective in reducing mammary tumor cell growth and viability than high dose monotherapy, suggesting that targeting multiple ErbB receptors with combination therapy may significantly improve the therapeutic response in breast cancer patients.
Stem cell fate dictated solely by altered nanotube dimension (2009)

Two important goals in stem cell research are to control the cell proliferation without differentiation and to direct the differentiation into a specific cell lineage when desired. Here, we demonstrate such paths by controlling only the nanotopography of culture substrates. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media. hMSC behavior in response to defined nanotube sizes revealed a very dramatic change in hMSC behavior in a relatively narrow range of nanotube dimensions. Small (approximately 30-nm diameter) nanotubes promoted adhesion without noticeable differentiation, whereas larger (approximately 70- to 100-nm diameter) nanotubes elicited a dramatic stem cell elongation (approximately 10-fold increased), which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for unique orthopedics-related hMSC treatments.
Design of High-Performance Regioregular Polythiophenes for Organic Thin-Film Transistors (2005)

The performance of a regioregular polythiophene semiconductor for solution-processed organic thin-film transistors (OTFTs) is critically dependent on its ability to self-organize from solution and its stability against p-doping by oxygen. Structural features that promote lamellar ordering and delicately curtail the /spl pi/-conjugation of regioregular polythiophene system would enable achievement of high field-effect mobility in air. Molecular design principles and structure-property correlation studies that lead to the design of a solution-processed, all-round high-performance polythiophene semiconductor system (PQT) for OTFTs are discussed. Mobility to 0.14 cm/sup 2/ V/sup -1/ s/sup -1/ and current on/off ratio of 10/sup 7/, together with other desirable thin-film transistor (TFT) properties have been obtained with this class of organic semiconductors in OTFTs under ambient fabrication conditions.
Full-color see-through daylight-readable goggle-mounted display (2011)

See-through near-to-eye displays offer an opportunity to present visual information laid on top of the view of the real world. The information presented can be used to augment or annotate the scene the user sees. Microvision has developed a see-through, full-color, daylight-readable, monocular display in a goggle form factor. The image source for the display is a Pico Display Engine (PDE) that uses modulated red, green, and blue lasers reflecting off a MEMS based bi-axial scanning mirror to create an image. This image is relayed to the eye through a pupil-expanding substrateguiding optic. The low Lagrange invariant laser-based display engine is an excellent match for the substrate guided optics for presenting the user with an infinite conjugate image. This paper discusses design considerations and performance characteristics of the eyewear display.