gebhart/patrepeval huggingface dataset:
Wound Fluids: A Window Into the Wound Environment? (2007)

Wound healing of the skin is a complex biologic process involving temporal interactions between numerous types of cells, extracellular matrix molecules, and soluble factors. The process of repair can be viewed as involving 3 or 4 phases: homeostasis, inflammation, synthesis, and remodeling. These phases occur at different times and differ in their cellular, biochemical, and physiologic requirements. Disruption of one or more of these interactions can significantly interfere with the repair process. Such comorbidities as age, nutrition, immune status, and underlying disease status (eg, diabetes or venous stasis) contribute additional intricacy to the repair process. Because of this complexity, care of chronic wounds remains highly individualized, and it should not come as a surprise that treatment of these wounds as a group with single target therapies have met with only modest success. A major hurdle in the progression toward improved treatment regimens has been the lack of objective biochemical and physiological landmarks that can be used to assess wound status. Collection and biochemical characterization of wound fluids presents the opportunity to noninvasively obtain information reflecting the status of the wound and of specific biomarkers. This review discusses the collection of wound fluid and highlights biomarkers that may be useful to this end.
The Prostaglandin Agonist Beraprost Aggravates Doxorubicin-mediated Apoptosis by Increasing iNOS Expression in Cardiomyocytes (2015)

Doxorubicin (DOX) is widely used as an anti-cancer agent although it causes irreversible cardiomyopathy by increasing oxidative stress and deregulating nitric oxide production. Beraprost (BPS), a stable prostacyclin (PGI2) analog, is a potent vasodilator that has beneficial effects on myocardial ischemia. The objectives of the present study were to delineate the uncertain effects of prostcyclin therapy on DOX induced cardiomyopathy and to explore the mechanisms underlying PGI2 and DOX interaction. For this reason, we stimulated endogenous PGI2 production using bicistronic COX-1/PGIS gene transfer and BPS supplementation, and investigated the effects on DOX-induced cardiomyopathy. Caspase-dependent protein content, lactate dehydrogenase (LDH), DNA fragmentation, and TUNEL positive cells were elevated in DOX-treated cardiomyocytes. These indicators were further elevated by adenovirus-COX- 1/PGIS transfection or BPS supplementation. In addition, PGI2 overexpression further increased iNOS expression and superoxide accumulation in cardiomyocytes compared with DOX alone, which may be the reason for aggravated cytotoxicity. Moreover, BPS can induce cAMP response elements (CRE) binding to the iNOS promoter and phospho- cAMP response element binding protein (CREB) expression in a cyclic AMP-dependent manner. Our in vivo studies show that MnTBAP and aminoguanidine treatment of DOX and BPS co-administered in mice can attenuate caspase-3 and PARP-1 protein expression, and improve mouse survival, as observed in the iNOS gene-deleted mice. In conclusion, we demonstrated that BPS or adv-COX-1/PGIS increases PGI2 levels through iNOS expression and peroxynitrite production, via CREB protein phosphorylation; thereby aggravating DOX-mediated cardiotoxicity.
Window management, graphics, and operating systems (1987)

In the past decade w e have witnessed a major shift in the defacto form of computing from central time shared systems to larger and larger networks of personal computing systems, Much of the SIGOPS literature in the past f e w years has forseen this) as witnessed by articles on extending operating systems so that resources can be easily used and shared in a networked based environment.
A Recombinant Mutant Vascular Endothelial Growth Factor-C that Has Lost Vascular Endothelial Growth Factor Receptor-2 Binding, Activation, and Vascular Permeability Activities (1998)

The vascular endothelial growth factor (VEGF) and the VEGF-C promote growth of blood vessels and lymphatic vessels, respectively. VEGF activates the endothelial VEGF receptors (VEGFR) 1 and 2, and VEGF-C activates VEGFR-3 and VEGFR-2. Both VEGF and VEGF-C are also potent vascular permeability factors. Here we have analyzed the receptor binding and activating properties of several cysteine mutants of VEGF-C including those (Cys156 and Cys165), which in other platelet-derived growth factor/VEGF family members mediate interchain disulfide bonding. Surprisingly, we found that the recombinant mature VEGF-C in which Cys156 was replaced by a Ser residue is a selective agonist of VEGFR-3. This mutant, designated DeltaNDeltaC156S, binds and activates VEGFR-3 but neither binds VEGFR-2 nor activates its autophosphorylation or downstream signaling to the ERK/MAPK pathway. Unlike VEGF-C, DeltaNDeltaC156S neither induces vascular permeability in vivo nor stimulates migration of bovine capillary endothelial cells in culture. These data point out the critical role of VEGFR-2-mediated signal transduction for the vascular permeability activity of VEGF-C and strongly suggest that the redundant biological effects of VEGF and VEGF-C depend on binding and activation of VEGFR-2. The DeltaNDeltaC156S mutant may provide a valuable tool for the analysis of VEGF-C effects mediated selectively via VEGFR-3. The ability of DeltaNDeltaC156S to form homodimers also emphasizes differences in the structural requirements for VEGF and VEGF-C dimerization.
Efficacy of Recombinant Glycoprotein D Subunit Vaccines on the Development of Primary, Recurrent, and Latent Genital Infections with Herpes Simplex Virus Type 2 in Guinea Pigs (1988)

Vaccination of guinea pigs with recombinant glycoprotein D (subunit vaccine) from either herpes simplex virus type 1 or type 2 (HSV-1, HSV-2), before intravaginal inoculation with HSV-2, markedly altered viral replication in the vaginal tract and reduced, or completely prevented, the development and spread of external genital lesions. Vaccination with glycoprotein D from HSV-2 prevented the establishment of latent HSV-2 infections in dorsal root ganglia and reduced the frequency of recurrent episodes. For all parameters of efficacy that we tested, vaccination with glycoprotein D from HSV-2 was more effective than was vaccination with protein derived from HSV-1. Vaccination after primary infection had no effect on the frequency or duration of recurrent HSV-2 infections.
Monolithic three-dimensional integration for memory scaling and neuromorphic computing (2015)

This paper explores some key applications and constraints for monolithic three-dimensional integration. We argue that there is a sweet spot at the few μm pitch level for applications that are extremely fault tolerant and repairable for wafer level stacking. We argue that memory especially DRAM and processing in memory are ideal applications and that if we are able to overcome the technology an architectural challenges wafer level Monolithic 3D has a promising future.
Fine scale 1-3 composites fabricated by the soft mold process: Preparation and modeling (2000)

Abstract The “soft mold” process offers the opportunity to fabricate fine scale 1-3 composites with free design of the piezoceramic rods. 1-3 composites with different volume fractions of arrayed PZT rods in a polymer matrix with diameters between 35 μm and 150 μm have been prepared successfully by this method. After poling the dielectric and electromechanical properties were determined. The experimental results were compared to calculated data from analytical approximations and modeling with the Finite Element Method (FEM).
Inflammatory Manifestations of Experimental Lymphatic Insufficiency (2006)

We have characterized a mouse model of acute, acquired lymphedema using in vivo functional imaging and histopathological correlation. The model closely simulates the volume response, histopathology, and lymphoscintigraphic characteristics of human acquired lymphedema, and the response is accompanied by an increase in the number and size of microlymphatic structures in the lymphedematous cutaneous tissues. Molecular characterization through clustering of genes with known functions provides insights into processes and signaling pathways that compose the acute tissue response to lymph stagnation. Further study of genes identified through this effort will continue to elucidate the molecular mechanisms and lead to potential therapeutic strategies for lymphatic vascular insufficiency.
The mobile ‘kill pill’ – poison or panacea? (2011)

As mobile devices have become key to the enterprise, the question of how best to secure them is hotly debated. Many enterprises have introduced remote disabling or wiping of devices, sometimes combining the two. But as employees increasingly use their own personal smart devices for work, often as part of a raft of mobile devices ranging from laptops through tablets to phones, the task of setting up remote disabling facilities across the enterprise can become Sisyphean. Tracey Caldwell reports on the technologies and solutions available for remote disabling of devices and the issues and considerations around remote disabling. The compromise between security and the ideal of the mobile enterprise is different for every business and is emerging as very much a business as well as a technological issue.
MIP1 DNA polymerase of S.<i>cerevisiae</i>: structural similarity with the<i>E.coli</i>DNA polymerase I-type enzymes (1991)

Peer reviewed
Serial transmission of human T‐cell leukemia virus type I by blood transfusion in rabbits and its prevention by use of X‐irradiated stored blood (1986)

Human T-cell leukemia virus type I (HTLV-I) was serially transmitted for 5 passages from rabbit to rabbit by blood transfusion. The virus could be transmitted with 20 ml of whole blood or washed blood cell suspension (fresh or stored for 1-2 weeks at 4 degrees C) but not with cell-free plasma from seroconverted rabbits. Seroconversion occurred 2-4 weeks after blood transfusion and serum anti-HTLV-I titers ranged from 1:20 to 1:640 with the immunofluorescence assay. From transfusion recipients of the 1st to 4th passages, virus-producing cell lines were established by culturing lymphocytes in the presence of T-cell growth factor (TCGF). Three of the 4 cell lines became TCGF-independent after 2-12 months of continuous culture. Blood was transfused between rabbits of opposite sexes and the recipient origin of each cell line was determined by chromosome analysis. We also investigated the effect of X-irradiation (6,000 rad) on blood from seropositive rabbits. Seroconversion likewise occurred in rabbits transfused with blood that had been irradiated immediately before transfusion but not in rabbits transfused with blood that had been irradiated and stored for 1-2 weeks at 4 degrees C. Thus, our rabbit model shows that HTLV-I is serially transmissible by blood transfusion and that this can be prevented by irradiation of blood. The same procedure, therefore, may be useful for the prevention of transfusion-related transmission of HTLV-I in humans.
Dense Gas Chromatography at Pressures to 2000 Atmospheres (1969)

Journal Article Dense Gas Chromatography at Pressures to 2000 Atmospheres Get access J. Calvin Giddings, J. Calvin Giddings Search for other works by this author on: Oxford Academic PubMed Google Scholar Marcus N. Myers, Marcus N. Myers Search for other works by this author on: Oxford Academic PubMed Google Scholar Jerry W. King Jerry W. King Department of Chemistry, University of Utah, Salt Lake City, Utah Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Chromatographic Science, Volume 7, Issue 5, May 1969, Pages 276–283, https://doi.org/10.1093/chromsci/7.5.276 Published: 01 May 1969 Article history Received: 28 October 1968 Published: 01 May 1969
Designing a Java microprocessor core using FPGA technology (2002)

Ever since its introduction from Sun Microsystems three years ago, Java has been widely accepted in the computing and Internet industry. However the run-time performance is still not good enough for Java to become a general purpose programming language. This paper shows how to implement a Java microprocessor core in silicon to speed up the execution of Java. For a reconfigurable and flexible design, the Field Programmable Gate Array (FPGA) is chosen as a target technology for the Java microprocessor. By applying a top-down hardware design methodology to the FPGA design process, it becomes easier and more flexible to implement Java in a FPGA. The DFT technique is added for better testability.
Block shuffling and adaptive interleaving for still image transmission over Rayleigh fading channels (1999)

A transmission system is proposed to enable reliable transmission of the vulnerable compressed images over fading channels. We introduce two techniques: (1) a block shuffling scheme which effectively isolates the erroneous picture blocks and thus improves the performance of error concealment at the decoder and (2) an adaptive interleaving scheme which adaptively changes the interleaving size such that bursty errors are randomized and become correctable by the embedded forward error control (FEC) scheme. The proposed system permits some well-known standards to be employed without modifying the source coding algorithms. Using JPEG coded images and simulated fading channels, we confirm the robustness of the proposed techniques over a wide range of operation conditions.
Inhibition of orexin-1/hypocretin-1 receptors inhibits yohimbine-induced reinstatement of ethanol and sucrose seeking in Long–Evans rats (2008)

The results suggest that inhibition of OX-1/Hcrt-1 receptors modulates operant ethanol self-administration and also plays a significant role in yohimbine-induced reinstatement of both ethanol and sucrose seeking in rats.
Characterization of Thin‐Film Rechargeable Lithium Batteries with Lithium Cobalt Oxide Cathodes (1996)

Thin‐film rechargeable lithium batteries with amorphous and crystalline cathodes were investigated. The lithium cobalt oxide films were deposited by radio‐frequency (RF) magnetron sputtering of an target in a 3:1 Ar/ mixture gas. From proton‐induced γ‐ray emission analysis (PIGE) and Rutherford backscattering spectrometry (RBS), the average composition of these films was determined to be or, within experimental uncertainty, . The x‐ray powder diffraction patterns of films annealed in air at 500 to 700°C were consistent with the regular hexagonal structure observed for crystalline . The discharge curves of the cells with amorphous cathodes showed no obvious structural transition between 4.2 and 2.0 V, while the discharge curves of the cells with polycrystalline cathodes were consistent with a two‐phase potential plateau at ∼3.9 V with a relatively large capacity. Two lower capacity plateaus were observed at ∼4.2 and 4.1 V with the 600 and 700°C annealed cathodes; the −dq/dV peaks were broader and weaker for the 600°C annealed cathodes and were not present at all with the 500°C annealed films. The chemical diffusion coefficients of in the cathodes obtained from ac impedance measurements at cell potentials of ∼4V ranged from ∼10−12 cm2/s for the as‐deposited amorphous cathodes to ∼10−9 cm2/s for the films annealed at 700°C. The capacity loss on extended cycling of the thin‐film cells varied with the crystallinity and thickness of the cathodes and with temperature. With the highly crystalline, 700°C annealed material, losses on cycling between 4.2 and 3.8 V at 25°C ranged from 0.0001%/cycle (>104 cycles) to 0.002%/cycle for cells with cathodes from 0.05 to 0.5 μm thick.
Down-regulation of homing receptors after T cell activation. (1988)

The specific pattern of lymphocyte localization and recirculation is important for the induction and expression of normal immune responses. In order to home to lymph nodes (LN), lymphocytes must first recognize and bind to specific high endothelial venules (HEV) in the LN. Binding to LN HEV is mediated by specific lymphocyte receptors, termed homing receptors, which are recognized by the mAb MEL-14. We examined the changes that occur in homing receptor expression after activation of murine T lymphocytes in vitro. Cells activated in MLC or by Con A undergo a 75% loss in their ability to recognize HEV, as demonstrated by a decrease in binding to HEV in vitro. Large, activated cells isolated from a primary MLC by elutriator centrifugation were completely unable to recognize HEV, whereas the small cells in the same culture continued to bind well. Flow cytometric analysis with MEL-14 showed that the activated fraction had lost expression of gp90MEL-14, the homing receptor Ag, whereas the inactivated cells remained MEL-14+. Concomitant with the loss of homing receptor expression, most of the activated cells became strongly peanut agglutinin (PNA)-positive, demonstrating a marked change in surface glycosylation. Thus, these MLC consist of two major populations of T cells--small, inactivated lymphocytes that are MEL-14+PNAlo and large, activated blast cells that are MEL-14-PNAhi. Purified MEL-14+ T cells activated by Con A gave rise to MEL-14- progeny, showing that gp90MEL-14 is lost from gp90MEL-14-positive precursors, rather than from the selective growth of MEL-14- cells. Furthermore, the loss of Ag expression on at least some activated cells is reversible in resting culture, with almost half of the cells reverting to MEL-14+ after the cessation of stimulation. These experiments show that activation of T cells results in down-regulation of surface homing receptors, resulting in their inability to recognize and bind to the endothelial surface of HEV. This suggests that the activation of T cells in vivo would result in a dramatic and physiologically significant change in their migration and localization properties which would be important during a normal immune response.
Cytokines and Lipopolysaccharide Induce Nitric Oxide Synthase in Cultured Rat Pulmonary Artery Smooth Muscle (1992)

In the current study, we describe cytokine and Escherichia coli lipopolysaccharide (LPS) induction of nitric oxide (NO) synthase mRNA levels in cultured smooth muscle from rat pulmonary artery (RPASM). Exposure of RPASM to interleukin-1 beta, interferon-gamma, or LPS alone did not significantly affect NO synthesis, as determined by nitrite concentrations in media. Exposure to tumor necrosis factor-alpha caused a modest (2x) increase in nitrite production. In contrast, exposure to a combination of the above three cytokines and LPS caused a large increase in NO synthesis. Exposure of RPASM to this combination caused an increase in mRNA levels of NO synthase (as described by Northern blot analysis with 32P-cDNA probe to an inducible form of NO synthase present in murine macrophages) that was apparent as early as 4 h. Expression of the induced gene product after exposure to the cytokine and LPS mixture was evident by significant increases in nitrite production at 12 h. Production of nitrite was completely abolished in the presence of NG-monomethyl-L-arginine (NMA), and this inhibition was reversible by the addition of excess L-arginine. NO synthase mRNA levels were not affected by NMA. The nitrite production induced by the combination of cytokines and LPS was abolished by pretreating cells with cycloheximide. These data indicate that a combination of cytokines and LPS affect expression of the gene for the inducible form of NO synthase in cultured RPASM.
A study on the effective road capacity and the modified road capacity: new evaluation parameters for traffic flow evaluation (2003)

In this paper effective road capacity and modified road capacity are proposed as new parameters for traffic flow evaluation. By using these parameters, possible impacts of an inter-vehicle communications network under autonomous cruising and platooning are evaluated. These evaluations are carried out using the interchange highway road traffic flow simulator developed by the authors. It is shown that an inter-vehicle communications network is effective for improving the modified road capacity.
Characterization of a Class of Cationic Peptides Able to Facilitate Efficient Protein Transduction in Vitro and in Vivo (2000)

Protein transduction domains (PTDs), such as the third helix of the Drosophila Antennapedia homeobox gene (Antp) and the HIV TAT PTD, possess a characteristic positive charge on the basis of their enrichment for arginine and lysine residues. To determine whether cationic peptides are able to function as protein transduction domains, 12-mer peptide sequences from an M13 phage library were selected for synthesis on the basis of their varying cationic charge content. In addition, polylysine and polyarginine peptides were synthesized in order to assess the effect of charge contribution in protein transduction. Coupling of the biotinylated peptides to avidin-beta-galactosidase facilitated transduction in a wide variety of cell lines and primary cells, including islet beta-cells, synovial cells, polarized airway epithelial cells, dendritic cells, myoblasts, and tumor cells. Two of the peptides, PTD-4 and PTD-5, mediated transduction nearly 600-fold more efficiently than a random control peptide, but with an efficiency similar to the TAT PTD and the 12 mers of polylysine and polyarginine. Furthermore, confocal analysis of biotinylated peptide-streptavidin-Cy3 conjugates demonstrated that the internalized PTDs are found in both the nuclei and the cytoplasm of treated cells. When tested in vivo, the PTDs were able to facilitate efficient and rapid protein delivery into rabbit synovium and mouse solid tumors following intraarticular and intratumoral administration, respectively. These novel PTDs can be used to transfer therapeutic proteins and DNA for the treatment of a wide variety of diseases, including arthritis and cancer.
Eotaxin Expression after Segmental Allergen Challenge in Subjects with Atopic Asthma (2001)

Expression of pulmonary eotaxin protein and mRNA was determined in six subjects with atopic asthma and five nonatopic normal subjects. Levels of eotaxin expression and eosinophil mobilization were compared before and after segmental allergen challenge in subjects with atopic asthma. In the absence of allergen challenge, we found significantly higher levels of eotaxin in the bronchoalveolar lavage (BAL) fluid of subjects with asthma than in that of normal subjects (25 +/- 3 versus 15 +/- 2 pg/ml, p < 0.05). BAL eotaxin levels increased after segmental allergen challenge in all six subjects with atopic asthma tested, with a mean increase from 22 +/- 4 to 53 +/- 10 pg/ml (p = 0.013). Segmental allergen challenge was associated with a significant increase in the percentage of BAL macrophages and eosinophils that were immunopositive for eotaxin. Eotaxin mRNA was detectable by northern analysis in BAL cells exclusively from allergen-challenged segments. Allergen- induced increases in eotaxin levels were strongly associated with increases in BAL eosinophil recovery (r(2) = 0.88, p = 0.0036). Segmental allergen challenge also increased eotaxin expression in airway epithelial and endothelial cells obtained by endobronchial biopsy. These findings demonstrate, for the first time, that the airways of subjects with allergic asthma respond to allergen by increasing eotaxin expression. The tissue loci of eotaxin expression, the levels of eotaxin recovered in BAL fluid, and the association of eotaxin levels with eosinophil mobilization suggest either that eotaxin plays a mechanistic role in allergen-induced airway eosinophilia or that it serves as a biomarker for the causal mechanisms.