gebhart/patrepeval huggingface dataset:
Chemical Probes of Signal-Transducing Proteins (2003)

Protein kinases are key participants in signal transduction pathways. A direct assessment of the relationship between the activity of any given protein kinase and the corresponding cellular phenotype has proven challenging. This is due to the large number of protein kinases encoded by the human genome coupled with intracellular temporal and spatial constraints that appear to further regulate the ultimate response of a cell to a stimulus. Our work has focused on the development of chemical probes to address the complexities associated with protein kinase-mediated cell signaling. These include the acquisition of highly selective substrates and inhibitors for specific members of the protein kinase family, the design and synthesis of light-activated signaling proteins and their corresponding inhibitors, and the preparation of fluorescent reporters of intracellular protein kinase action.
Characterization of back-channel subthreshold conduction of walled SOI devices (1991)

Submicrometer CMOS transistors with junctions walled to a trenched isolation oxide have been fabricated on SIMOX substrates. Back-channel leakage of the devices was measured as a function of substrate bias. Compared with nonwalled devices, walled devices showed early turn-on of the back-channel at relatively low substrate biases. This phenomenon is caused by the two-dimensional electrical field effect at the bottom corner of the device sidewalls, and is supported by PISCES simulation.
The histidine utilization (hut) genes of Pseudomonas fluorescens SBW25 are active on plant surfaces, but are not required for competitive colonization of sugar beet seedlings (2006)

The ability to monitor the spatial and temporal distribution of signals in complex environments is necessary for an understanding of the function of bacteria in the wild. To this end, an existing recombinase-based transcriptional reporter strategy (recombinase-based in vivo expression technology, RIVET) has been extended and applied to the plant-colonizing bacterium Pseudomonas fluorescens SBW25. Central to the project was a rhizosphere-inducible locus, rhi14, which functional analyses show is hutT, a histidine-inducible gene that is required for histidine utilization. A transcriptional fusion between hutT and a promoterless site-specific recombinase (tnpR(mut168)) results in excision of a chromosomally integrated tetracycline-resistance cassette in a histidine-dependent manner. The dose- and time-responsiveness of the promoterless recombinase to histidine closely mirrored the histidine responsiveness of an identical hutT fusion to promoterless lacZ. To demonstrate the effectiveness of the strategy, the activity of hutT was monitored on sugar beet seedlings. Low levels of transcriptional activity were detected in the phyllosphere, rhizosphere and in plant extract, but not in vermiculite devoid of seedlings. The histidine concentration in the rhizosphere was estimated to be 0.6 microg ml(-1). The ecological significance of the hut locus was examined by competing a hutT deletion mutant against the wild-type during colonization of sugar beet seedlings. No impact on competitive fitness was detected, suggesting that the ability to utilize plant-derived histidine is not essential for bacterial colonization.
Mobile to Mobile data transfer through Human Area Network (2013)

Technologies enabling communication between people and devices in close proximity are required for all modern user friendly gadgets. This paper demonstrates a design and implementation of Human Area Network technology that enables communication through human contact. Communication between mobile terminals and terminals that are embedded in the environment has become important. Use of cables is inconvenient as they can get tangled and are difficult to manage when used in Communication between devices which are in close proximity. When very weak radio signals are used for the communication, data speeds are reduced by packet collision in crowded places such as exhibition sites. There is also security risk from unwanted signal interception. For solving such problems we use body of a person as a signal path for communication. A transmission path is formed automatically when a person comes into contact with a device and communications between mobile terminals begin. The hardware setup for this paper contains Transmitter and Receiver unit. These units contain BT-42, 32 bit ARM 7 TDMI Processor, LM 358 and Power supply.  This platform consumes less power and it is cost effective. This concept will reduce load on other communication channels by introducing new communication mode.
Stimulation of canine hematopoiesis by recombinant human granulocyte-macrophage colony-stimulating factor. (1989)

The effect of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on canine hematopoiesis was evaluated. rhGM-CSF stimulated granulocyte-macrophage colony formation of canine marrow depleted of accessory cells up to tenfold. Stimulation of colony formation was abrogated by anti-rhGM-CSF antiserum or heat inactivation. rhGM-CSF also stimulated in vivo canine hematopoiesis both when given as continuous i.v. infusion and as intermittent s.c. injections. Neutrophil, monocyte, and lymphocyte counts were increased three- to eightfold above controls, whereas values for eosinophils, reticulocytes, and hematocrits were not changed. Bone marrow histology after 2 weeks of treatment with rhGM-CSF showed hypercellularity with myeloid hyperplasia and left-shifted granulocytopoiesis. After discontinuation of rhGM-CSF, peripheral leukocyte counts returned to control level within 3-7 days. Platelet counts decreased rapidly after starting rhGM-CSF, to 5000-15,000 platelets/mm3, and increased within 24 h after stopping rhGM-CSF treatment, whereas marrow histology after 2 weeks of rhGM-CSF application showed the normal number and morphology of megakaryocytes.
The mosaic of autoimmunity: prediction, autoantibodies, and therapy in autoimmune diseases--2008. (2008)

For more than two decades, the detection of serum autoanti-bodies has been used for the diagnosis and classification of autoimmune diseases. In addition, some autoantibodies have a prognostic significance or are used as markers for disease activity. In recent years a new piece in the mosaic of autoimmunity has clearly emerged – namely, the predictive value of autoantibodies. Indeed, many autoantibodies can be detected in the preclinical phase of autoimmune diseases many years before the disease becomes apparent; furthermore, they have a high diagnostic positive predictive value [1].Among autoimmune rheumatic diseases, antibodies to RNP, Sm, dsDNA, cardiolipin, Ro and La have a PPV for systemic lupus erythematosus of 94–100%. According to the type of antibody, the appearance can precede clinical diagnosis by 7–10 years with a frequency that varies from 32% to 78% at the moment of
Multiple variants of the human lymphocyte homing receptor CD44 generated by insertions at a single site in the extracellular domain. (1992)

The human CD44 cell-surface glycoprotein participates in a wide variety of cell-cell interactions including lymphocyte homing and tumor metastasis. The CD44 antigen is known to display extensive size heterogeneity when compared between different tissue sources although the structural basis for this variation is not yet clear. Recently, two further isotypes in addition to the basic hemopoietic form of the CD44 antigen have been cloned and sequenced and these have been found to contain all or part of a 200-400-base pair insert within the extracellular domain, suggesting that the characteristic heterogeneity in the molecule may be generated by a mechanism of alternative splicing. We have obtained further evidence for alternative splicing, and we report here the cloning and sequencing of six different CD44 sequence variants from a variety of cell lines using a combination of expression cloning and the polymerase chain reaction. Comparison of these variants indicates that each is probably assembled by the insertion of five different exon units in tandem into a discrete site within the membrane proximal region of the extracellular domain. One of the variants contains an exon that shares extensive amino acid sequence homology with a recently described rat CD44 variant that mediates tumor metastasis. Another variant contains a new exon that encodes a tandem repeat of the consensus sequence SG for covalent modification with chondroitin sulfate and is expressed predominantly on mammary tumors. We suggest that a mechanism of alternative exon splicing generates much of the observed structural heterogeneity of CD44 and that the particular set of CD44 variants expressed in a single cell may represent a precise postal code directing the final destination of migrating cells and metastatic tumors.
Across the Disciplines (2001)

Abstract The authors describe how subsidized, self-service document delivery at the University of Tennessee Library meets researchers' needs with emphasis on comparing delivery service performance by users' discipline. Analysis of use reports from the document delivery vendor (UnCover) and a user survey are the major components of the study presented here. Recognizing that document delivery is like other elements of collection management in that needs vary by discipline, the researchers also acknowledge that libraries never achieve equity in meeting information needs in all fields. The authors set out to test the assumption that researchers in the sciences benefited from a subsidized document delivery service more than those in the social sciences and humanities. Overall satisfaction rates were high across disciplines, while awareness and use was highest among science researchers. Librarians at the University of Tennessee concluded that the Un-Cover@UTK service is useful as one alternative to local ownership of many journals, despite recognized limitations such as article availability, copyright fees, and variable fax quality.
Understanding the Hydration Process of Salts: The Impact of a Nucleation Barrier (2019)

The solid-state hydration of salts has gained particular interest within the frame of thermochemical energy storage. In this work, the water vapor pressure–temperature (p–T) phase diagram of the following thermochemical salts was constructed by combining equilibrium and nonequilibrium hydration experiments: CuCl2, K2CO3, MgCl2·4H2O, and LiCl. The hydration of CuCl2 and K2CO3 involves a metastable zone of ca. 10 K, and the induction times preceding hydration are well-described by classical homogeneous nucleation theory. It is further shown for K2CO3 (metastable) and MgCl2·4H2O (not metastable) through solubility calculations that the phase transition is not mediated by bulk dissolution. We conclude that the hydration proceeds as a solid–solid phase transition, mobilized by a wetting layer, where the mobility of the wetting layer increases with increasing vapor pressure. In view of heat storage application, the finding of metastability in thermochemical salts reveals the impact of nucleation and growth processes on the thermochemical performance and demonstrates that practical aspects like the output temperature of a thermochemical salt are defined by its metastable zone width (MZW) rather than its equilibrium phase diagram. Manipulation of the MZW by e.g. prenucleation or heterogeneous nucleation is a potential way to raise the output temperature and power on material level in thermochemical applications.
A Simulation of AE Continuous Monitoring Systems to Estimate Degradation of Structures (1983)

One of the most important applications of AE technology is continuous monitoring of structures and real-time estimation of their integrity. The quantitative evaluation of the monitoring performance according to applied objects and environmental conditions is necessary. The performance evaluation can determine quantitatively the trade-off between the estimation reliability of integrity and monitoring costs.A model for the AE monitoring of structures has been developed, which is composed of submodels such as fracture, AE generation, propagation, measurement and estimation of degradation. The model is thought to be useful for the estimation of fracture behavior in structures from detected AE waves. In this paper, examples of sub-models and calculation method applied to the fatigue degradation are given. Following conclusions have been obtained from simulation results;1) Based on the data obtained by material tests, the integrity of structures could be estimated from the relatively small number of AE events detected.2) The upper limit of monitoring area is calculable for various AE generation patterns and stress levels.
Arginine Deficiency Accompanies Persistent Pulmonary Hypertension of the Newborn (1994)

Persistent pulmonary hypertension of the newborn (PPHN) is a life-threatening disorder of neonates manifested by vasoconstriction of the pulmonary arteries. Recently, the gas nitric oxide (NO) has been used with some success in the management of infants with PPHN. Exogenous administration of NO selectively dilates the pulmonary vascular bed. NO is naturally synthesized in the body from the amino acid L-arginine. Here we report our findings that infants with PPHN are deficient in arginine and achieve normal or elevated plasma arginine concentrations with intravenous hyperalimentation. We prospectively identified and studied 10 infants with PPHN who were not receiving protein or amino acids for at least 24 h and compared their plasma arginine concentrations to 8 control infants without PPHN given similar nutrition. Plasma arginine concentrations were 32 +/- 14 and 52 +/- 20 mumol/l in infants with PPHN and control infants, respectively (p = 0.02). There were no other statistically significant differences in plasma amino acid concentration for any of the 22 other amino acids determined. Infants with PPHN who were subsequently treated with amino acid infusions had plasma arginine concentrations of 115 +/- 48 mumol/l (mean of ten determinations at 86 +/- 27 h after initiation of intravenous amino acids in five PPHN infants).
Effects of an Anti–VEGF-A Monoclonal Antibody on Laser-Induced Choroidal Neovascularization in Mice: Optimizing Methods to Quantify Vascular Changes (2008)

Because of easy execution, low background fluorescence, and detailed visualization of new vessels, intravital injection of tomato lectin followed by a quantification based on threshold fluorescence represents the best technique for measuring CNV and the vascular changes induced by anti-VEGF-A monoclonal antibody in mice.
Flexible power scheduling for sensor networks (2004)

We propose a distributed on-demand power-management protocol for collecting data in sensor networks. The protocol aims to reduce power consumption while supporting fluctuating demand in the network and provide local routing information and synchronicity without global control. Energy savings are achieved by powering down nodes during idle times identified through dynamic scheduling. We present a real implementation on wireless sensor nodes based on a novel, two-level architecture. We evaluate our approach through measurements and simulation, and show how the protocol allows adaptive scheduling and enables a smooth trade-off between energy savings and latency. An example current measurement shows an energy savings of 83% on an intermediate node.
Developmental tumourigenesis: NCAM as a putative marker for the malignant renal stem/progenitor cell population (2008)

During development, renal stem cells reside in the nephrogenic blastema. Wilms' tumour (WT), a common childhood malignancy, is suggested to arise from the nephrogenic blastema that undergoes partial differentiation and as such is an attractive model to study renal stem cells leading to cancer initiation and maintenance. Previously we have made use of blastema-enriched WT stem-like xenografts propagated in vivo to define a 'WT-stem' signature set, which includes cell surface markers convenient for cell isolation (frizzled homolog 2 [Drosophila] - FZD2, FZD7, G-protein coupled receptor 39, activin receptor type 2B, neural cell adhesion molecule - NCAM). We show by fluorescenceactivated cell sorting analysis of sphere-forming heterogeneous primary WT cultures that most of these markers and other stem cell surface antigens (haematopoietic, CD133, CD34, c-Kit; mesenchymal, CD105, CD90, CD44; cancer, CD133, MDR1; hESC, CD24 and putative renal, cadherin 11), are expressed in WT cell sub-populations in varying levels. Of all markers, NCAM, CD133 and FZD7 were constantly detected in low-to-moderate portions likely to contain the stem cell fraction. Sorting according to FZD7 resulted in extensive cell death, while sorted NCAM and CD133 cell fractions were subjected to clonogenicity assays and quantitative RT-PCR analysis, exclusively demonstrating the NCAM fraction as highly clonogenic, overexpressing the WT 'stemness' genes and topoisomerase2A (TOP2A), a bad prognostic marker for WT. Moreover, treatment of WT cells with the topoisomerase inhibitors, Etoposide and Irinotecan resulted in down-regulation of TOP2A along with NCAM and WT1. Thus, we suggest NCAM as a marker for the WT progenitor cell population. These findings provide novel insights into the cellular hierarchy of WT, having possible implications for future therapeutic options.
Use of restriction endonucleases for mapping the allele for beta s-globin. (1982)

We have reported the direct analysis of the allele for beta 2-globin by using restriction endonuclease Dde I coupled with blot-hybridization analysis. In that report we predicted that a major use of our analysis could be for the prenatal diagnosis of sickle cell anemia. Here we present such an analysis. In addition, this report also describes the use of a new enzyme Mst II, which also distinguish the beta s allele from the normal beta-globin allele. Blot-hybridization analysis with restriction endonuclease Mst II shows the 5' end of the normal beta-globin gene to reside on a fragment of approximately 1.14 kilobases, whereas the 5' end of the beta s-globin gene resides on a fragment of approximately 1.34 kilobases. Because the fragment sizes generated by Mst II are significantly larger than those generated by Dde I, one can easily perform a prenatal diagnosis for sickle cell by standard blot hybridizations onto nitrocellulose filters.
Classical hardness of learning with errors (2013)

We show that the Learning with Errors (LWE) problem is classically at least as hard as standard worst-case lattice problems. Previously this was only known under quantum reductions.
Phosphatidylserine-Specific Receptor Contributes to TGF-.BETA. Production in Macrophages through a MAP Kinase, ERK (2005)

We explored the involvement of the phosphatidylserine (PS)-receptor in the production of TGF-beta by macrophages treated with PS-liposomes. The binding of anti-PS-receptor antibody to macrophages was specifically inhibited by PS-liposomes. The antibody led to an increase in the production of TGF-beta, and also activated ERK, a member of the MAP kinase. But no activations in p38 and JNK were observed. ERK inhibitor, U0126 completely prevented TGF-beta production. On the addition of a TGF-beta neutralizing antibody or U0126, the inhibitory effect of the anti-PS-receptor antibody on macrophage function, nitric oxide production, was restored. These findings suggested that TGF-beta is one of factors produced by PS-liposomes, and the ERK signaling pathway via the PS-receptor is intimately involved in the production of TGF-beta in macrophages.
Myristylation alters DNA-binding activity and transactivation of FBR (gag-fos) protein. (1991)

FBR murine sarcoma virus (gag-fos) protein, a virally transduced Fos protein, exhibits decreased gene transactivation in comparison with the cellular Fos protein. Biochemical analysis suggests that myristylation of the virally encoded N-terminal gag region results in decreased DNA binding and transcriptional activation without affecting heterodimerization with Jun protein. These findings demonstrate that protein myristylation can modulate gene regulation by a DNA-binding protein.
Enterotoxigenic Bacteroides fragilis: A potential instigator of colitis (2007)

ETBF, a colonic commensal, alone stimulates colitis and significantly enhances colonic inflammation in DSS-treated mice. This study suggests that acquisition of ETBF colonization may be a potential factor in initiation and/or exacerbation of colitis.
Increased heart rate as a risk factor for cardiovascular disease (2003)

European Heart Journal Supplements ( Its pharmacological modulation is increasingly the focus of therapeutic approaches to alleviate symptoms and prolong survival. It is the simplest cardiovascular variable to measure accurately and reproducibly. Many long-term follow-up studies suggest that elevated heart rate increases all-cause mortality, cardiovascular disease and sudden death in patients with known or suspected coronary heart disease, survivors of myocardial infarction and patients with hypertension. These links hold for men and women, and are unrelated to ethnic origin. The effect on sudden death or total mortality increases as a function of heart rate such that an increase in heart rate by more than 40 beats/min doubles total mortality. Conversely, low heart rate reduces risk for coronary artery disease and sudden death. Primary and secondary prevention studies in myocardial infarction indicated that elevated heart rate in susceptible patients predicts risk for developing myocardial infarction and death. Prophylactic beta-blockers attenuate risks for reinfarction, sudden death and total mortality; these effects correlate with reduced heart rate, thus providing a compelling basis for developing agents that reduce heart rate exclusively as antianginal agents for the therapy of myocardial ischaemia with broader therapeutic implications.
CMOS active pixel image sensor (1994)

A new CMOS active pixel image sensor is reported. The sensor uses a 2.0 /spl mu/m double-poly, double-metal foundry CMOS process and is realized as a 128/spl times/128 array of 40 /spl mu/m/spl times/40 /spl mu/m pixels. The sensor features TTL compatible voltages, low noise and large dynamic range, and will be useful in machine vision and smart sensor applications.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Clinical and technical considerations for imaging colorectal cancers with technetium-99m-labeled antiCEA Fab' fragment. (2000)

Colorectal cancer is the third most common cancer, after lung and breast cancers. Approximately 133,500 Americans develop colorectal cancer annually and approximately 54,900 die of the disease. As many as 600,000 individuals in the US are under care after surgery for colorectal cancer. After reading this article, the nuclear medicine technologist will be able to: (a) describe the role of Arcitumomab in evaluating and managing patients with recurrent colorectal carcinoma metastasizing to the liver; (b) discuss the clinical use of CEA-Scan (Immunomedics, Inc., Morris Plains, NJ) and its overall imaging performance characteristics and sensitivity related to specific anatomical sites compared to conventional diagnostic modalities; (c) describe radiopharmaceutical preparation and quality control; (d) identify the pertinent patient history before starting the test; and (e) explain the imaging procedure, processing and display of data to optimize study interpretation.