gebhart/patrepeval huggingface dataset:
Toward rules relating zinc finger protein sequences and DNA binding site preferences. (1992)

Zinc finger proteins of the Cys2-His2 type consist of tandem arrays of domains, where each domain appears to contact three adjacent base pairs of DNA through three key residues. We have designed and prepared a series of variants of the central zinc finger within the DNA binding domain of Sp1 by using information from an analysis of a large data base of zinc finger protein sequences. Through systematic variations at two of the three contact positions (underlined), relatively specific recognition of sequences of the form 5'-GGGGN(G or T)GGG-3' has been achieved. These results provide the basis for rules that may develop into a code that will allow the design of zinc finger proteins with preselected DNA site specificity.
Identification of &lt;i&gt;Blomia tropicalis&lt;/i&gt; Allergen Blo t 5 by cDNA Cloning (1995)

Made available in DSpace on 2018-06-15T17:44:11Z (GMT). No. of bitstreams: 0 Previous issue date: 1995-05-01
MIL-101(Fe) as a lithium-ion battery electrode material: a relaxation and intercalation mechanism during lithium insertion (2015)

The electrochemical performance and mechanism of a MIL-101(Fe) metal–organic framework (MOF) as a lithium ion battery electrode is reported for the first time.
Resistance of HIV-1 to multiple antiretroviral drugs in France: a 6-year survey (1997-2002) based on an analysis of over 7000 genotypes. (2003)

This study demonstrates the high prevalence of drug-resistant virus in a European cohort of patients currently under treatment for HIV-1 infection. New therapeutic strategies based on a more rational use of genotypic data should be worked out to prevent the development of resistance.
Dynamic linking of software components (1997)

Traditionally, dynamic linkers merely combined previously compiled pieces of code. Faster processors are now making outright code generation at load time practical, leading to cross-platform portability at very little extra cost. While the central idea behind dynamic linking is quite straightforward, it can be implemented through a surprising variety of strategies. The author contrasts three simple dynamic linking schemes with two much more elaborate strategies. The latter represent a workload shift from the compiler to the dynamic linker, just as dynamic linking itself represents a shift that moves the functions of a separate linker into the loader. Because the new techniques promise the profound additional benefit of cross-platform portability, they will most likely displace the currently popular linking-loader approach.
High-affinity Activators of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Chloride Conductance Identified by High-throughput Screening (2002)

Cystic fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR) protein that reduce cAMP-stimulated Cl(-) conductance in airway and other epithelia. The purpose of this investigation was to identify new classes of potent CFTR activators. A collection of 60,000 diverse drug-like compounds was screened at 10 microm together with a low concentration of forskolin (0.5 microm) in Fisher rat thyroid epithelial cells co-expressing human CFTR and a green fluorescent protein-based Cl(-) sensor. Primary screening yielded 57 strong activators (greater activity than reference compound apigenin), most of which were unrelated in chemical structure to known CFTR activators, and 284 weaker activators. Secondary analysis of the strong activators included analysis of CFTR specificity, forskolin requirement, transepithelial short-circuit current, activation kinetics, dose response, toxicity, and activation mechanism. Three compounds, the most potent being a dihydroisoquinoline, activated CFTR by elevating cellular cAMP, probably by phosphodiesterase inhibition. Fourteen compounds activated CFTR without cAMP elevation or phosphatase inhibition, suggesting direct CFTR interaction. The most potent compounds had tetrahydrocarbazol, hydroxycoumarin, and thiazolidine core structures. These compounds induced CFTR Cl(-) currents rapidly (<5 min) with K(d) down to 200 nm and were CFTR-selective, reversible, and nontoxic. Several compounds, the most potent being a trifluoromethylphenylbenzamine, activated the CF-causing mutant G551D, but with much weaker affinity (K(d) > 10 microm). When added for 10 min, none of the compounds activated DeltaPhe(508)-CFTR in transfected cells grown at 37 degrees C (with DeltaPhe(508)-CFTR trapped in the endoplasmic reticulum). However, after correction of trafficking by 48 h of growth at 27 degrees C, tetrahydrocarbazol and N-phenyltriazine derivatives strongly stimulated Cl(-) conductance with K(d) < 1 microm. The new activators identified here may be useful in defining molecular mechanisms of CFTR activation and as lead compounds in CF drug development.
Established and Novel Biomarkers of Sepsis (2011)

The increased incidence of sepsis, a systemic response to infection that occurs in some patients, has stimulated interest in identifying infected patients who are at risk and intervening early. When this condition progresses to severe sepsis (characterized by organ dysfunction), mortality is high. Hospitals that have implemented recommendations of the Surviving Sepsis Campaign have seen a reduction in mortality rate for hospital-acquired severe sepsis. They may reduce this further by focusing on new approaches to diagnosing sepsis, especially at an early stage. Sepsis is a complicated syndrome with many physiological derangements and many emerging laboratory markers of sepsis have been proposed as adjuncts to clinical evaluation. The list includes cytokines, acute phase proteins, neutrophil activation markers, markers of abnormal coagulation and, recently, markers of suppression of both the innate and adaptive immune response. The perfect biomarker would accurately identify patients at risk of developing severe sepsis and then guide targeted therapy.
Simultaneous Determination of α-Fetoprotein and Free β-Human Chorionic Gonadotropin by Element-Tagged Immunoassay with Detection by Inductively Coupled Plasma Mass Spectrometry (2004)

Two proteins can be measured simultaneously by immunoassays using two rare earth elemental tags (Eu(3+) and Sm(3+)) and ICP-MS detection. The multielement capability and the multiple potential elemental labels make ICP-MS attractive for multianalyte immunoassays. Implementation of ICP-MS-linked immunoassays may be relatively straightforward because the labeling and immunoreaction procedures have been well developed for clinical time-resolved immunofluorometric assays.
Synthesis of unusual perfluorocarbon ethers and amines containing bulky fluorocarbon groups: new biomedical materials (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of unusual perfluorocarbon ethers and amines containing bulky fluorocarbon groups: new biomedical materialsHsu Nan Huang, Daniel F. Persico, Richard J. Lagow, and Leland C. Clark Jr.Cite this: J. Org. Chem. 1988, 53, 1, 78–85Publication Date (Print):January 1, 1988Publication History Published online1 May 2002Published inissue 1 January 1988https://pubs.acs.org/doi/10.1021/jo00236a016https://doi.org/10.1021/jo00236a016research-articleACS PublicationsRequest reuse permissionsArticle Views475Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Health Care Utilization in the 10 Years Prior to Diagnosis in Obstructive Sleep Apnea Syndrome Patients (1999)

Obstructive sleep apnea syndrome (OSAS) patients may have symptoms for years prior to recognition of their disorder, or they may be treated for the associated comorbidities. We hypothesized that such patients would be heavy consumers of health care resources for several years prior to diagnosis. We therefore compared health service utilization for a 10-year interval prior to diagnosis of 181 OSA patients to those of randomly selected age-, gender-, and geographically matched controls from the general population. OSAS patients used approximately twice as many health care services (as defined by physician claims and overnight stays in hospital) in the 10 years prior to their initial diagnostic evaluation for apnea. Physician claims for the OSA patients totaled $686,365 ($3972 per patient), compared to $356,376 ($1969 per patient) for the controls for the 10-year period examined in this study. Use of health services was significantly higher in 7 of 10 years prior to diagnosis. The OSAS patients also had more overnight hospitalizations: they spent 1118 nights (6.2 per patient) in hospital vs 676 nights (3.7 per patient) for controls in the decade prior to diagnosis. We conclude that by the time patients are finally diagnosed for sleep apnea, they have already been heavy users of health services for several years. It is possible that our findings reflect not OSAS per se, but the presence of some of the risk factors that predispose to OSAS, such as obesity, alcohol usage and perhaps tobacco consumption.
Self-limitation of AlGaN/GaN quantum well energy by built-in polarization field (1999)

Wurtzite AlGaN/GaN quantum well (QW) structures were grown by molecular beam epitaxy on c-plane sapphire substrates and the QW transition energies were measured by low temperature photoluminescence. Both the well widths and the Al mole fraction in the AlxGa1−xN(0&amp;lt;x&amp;lt;0.3) 100-Å-thick barriers have been varied in order to assess the built-in electric field present in the quantum heterostructures. It is found that the electric field increases linearly with the Al composition. The magnitude of this electric field is as high as 1 MV/cm for an Al mole fraction of 0.27. The main consequence is that whatever the investigated Al composition range, the well thickness must be lower than 30 Å in order to get a transition energy greater than the band gap of GaN.
Urine citrate and renal stone disease. (1989)

Calcium stone disease is attributable to supersaturation of the urine with calcium and other salts, the presence of substances that promote crystallization and a deficiency of inhibitors of crystallization. Citrate is a potent inhibitor of calcium oxalate and calcium phosphate stone formation whose excretion is diminished in some patients with stone disease owing to idiopathic causes or secondary factors such as bowel disease and use of thiazides. The pH within the proximal tubule cells is an important determinant of citrate excretion. Multivariate analysis has shown that the urine concentrations of calcium and citrate are the most important factors in stone formation. In uncontrolled studies potassium citrate, which increases urinary citrate excretion, appears to be promising as a therapeutic agent for patients with stone disease and hypocitraturia refractory to other treatment. On the other hand, there are potential drawbacks to sodium alkali therapy, such as the precipitation of calcium phosphates.
Urinary excretion of pyridinium cross‐links in healthy women; the long‐term effects of menopause and oestrogen/progesterone therapy (1994)

Both Pyr/Cr and D-Pyr/Cr, two new markers of bone resorption, increased significantly at the time of the menopause, thereafter remaining fairly constant during the first post-menopausal decade. Women with increased bone loss continue to have elevated urinary excretion of pyridinium cross-links during the first decade of the menopause. This post-menopausal change is reversed by hormone replacement therapy to the premenopausal level.
Security testing of high impedance fault detectors (2002)

The security of high-impedance fault (HIF) relays is examined. Waveforms of HIFs as well as loads that behave to appear like HIFs were collected and processed. The detection parameters used in a number of present HIF detection algorithms were extracted and compared to test the algorithms' ability to discriminate between faults and fault-like loads. It was found that there are loads that imitate HIFs, and that the present algorithms lack the security required under these load conditions. The high-impedance fault-like loads considered were: an arc welding machine; a personal computer; and a fluorescent light. It was found that arc welders can easily imitate HIF conditions, and that the tested algorithms showed insecurity when tested with an arc welder load.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Table Understanding for Information Retrieval (2002)

This thesis proposes a novel approach for finding tables in text files containing a mixture of unstructured and structured text. Tables may be arbitrarily complex because the data in the tables may themselves be tables and because the grouping of data elements displayed in a table may be very complex. Although investigators have proposed competence models to explain the structure of tables, there are no computationally feasible performance models for detecting and parsing general structures in real data. Our emphasis is placed on the investigation of a new statistical procedure for detecting basic tables in plain text documents. The main task here is defining and testing this theory in the context of the Odessa Digital Library. Acknowledgements I would like to express my gratitude to my advisor, Dr. Roger Ehrich, for his support, advice and encouragement. Without his tireless reading and constructive criticism, this work would not have been possible. I would also like to thank my thesis committee, Dr. Edward Fox and Dr. Christopher North
Brain Neurotrauma: Molecular, Neuropsychological, and Rehabilitation Aspects (2015)

Every year, an estimated 1.7 million Americans sustain brain injury. Long-term disabilities impact nearly half of moderate brain injury survivors, and nearly 50,000 of these cases result in death, with an approximate $76.5 billion being spent on direct and indirect medical costs.Over the last decade, the field of neurotrauma has witnessed significant advances, especially at the molecular, cellular, and behavioral levels. This progress is largely due to the introduction of novel techniques, as well as the development of new animal models of central nervous system (CNS) injury.Written by more than one hundred globally renowned experts in the field of CNS injury, Brain Neurotrauma: Molecular, Neuropsychological, and Rehabilitation Aspects provides a comprehensive and up-to-date account of the latest developments in the area of neurotrauma, including biomarker development, experimental models, diagnostic methods, and therapeutic interventions in brain injury research.
Picotesla Magnetic Sensors for Low-Frequency Applications (2011)

We demonstrate a simple low-power, magnetic sensor system suitable for high-sensitivity magnetic-field mapping, based on solid-state magnetic tunnel junction devices with minimum detectable fields in a 100 pT range at room temperature. In this paper, we discuss a method that uses multilayer thin films to improve the performance of the soft ferromagnetic layer in magnetoresistive sensor applications, by reducing the coercivity and/or improving the reversibility. We have used it in the design of our new magnetic sensor. This sensor has a sensitivity as high as 750%/mT. The magnetic sensor only dissipates 1 mW of power while operating under an applied voltage of 1 V.
Multiple components of the A1 adenosine receptor-adenylate cyclase system are regulated in rat cerebral cortex by chronic caffeine ingestion. (1988)

The effects of chronic caffeine on the A1 adenosine receptor-adenylate cyclase system of rat cerebral cortical membranes were studied. Caffeine treatment significantly increased the number of A1 adenosine receptors as determined with the A1 adenosine receptor antagonist radioligand [3H]xanthine amine congener (XAC). R-PIA (agonist) competition curves constructed with [3H]XAC were most appropriately described by a two affinity state model in control membranes with a KH of 2.1 +/- 0.8 and a KL of 404 +/- 330 nM with 50 +/- 4% of receptors in the high affinity state (%RH). In contrast, in membranes from treated animals, there was a marked shift towards the high affinity state. In three of seven animals all of the receptors were shifted to a unique high affinity state which was indistinguishable from the KH observed in membranes from control animals. In four of seven animals the %RH increased from 50 to 69% with KH and KL indistinguishable from the control values. Thus, the agonist specific high affinity form of the receptor was enhanced following caffeine treatment. Maximal inhibition of adenylate cyclase activity in cerebral cortical membranes by R-PIA (1 microM) was significantly increased by 28% following caffeine treatment, consistent with an increased coupling of receptor-Gi protein with adenylate cyclase. Importantly, the quantity of Gi (alpha i) in rat cerebral cortex, determined by pertussis toxin-mediated labeling, was also increased to 133% of control values by this treatment. Thus, multiple components and interactions of the A1 adenosine receptor-adenylate cyclase complex are regulated by caffeine. These changes are likely compensatory measures to offset blockade of A1 receptors in vivo by caffeine and lead to a sensitization of this inhibitory receptor system.
Combining Prosodic Lexical and Cepstral Systems for Deceptive Speech Detection (2006)

We report on machine learning experiments to distinguish deceptive from nondeceptive speech in the Columbia-SRI-Colorado (CSC) corpus. Specifically, we propose a system combination approach using different models and features for deception detection. Scores from an SVM system based on prosodic/lexical features are combined with scores from a Gaussian mixture model system based on acoustic features, resulting in improved accuracy over the individual systems. Finally, we compare results from the prosodic-only SVM system using features derived either from recognized words or from human transcriptions.
MIXED CHIMERISM OF THE RESIDENT MACROPHAGE POPULATION AFTER ALLOGENEIC BONE MARROW TRANSPLANTATION FOR CHRONIC MYELOID LEUKEMIA1 (2002)

The CD68+ resident bone marrow macrophage population including PGCs are involved in the lineage-specific chimerism and minimal residual disease after BMT in CML.
Gaze correction with a single webcam (2014)

Eye contact is a critical aspect of human communication. However, when talking over a video conferencing system, such as Skype, it is not possible for users to have eye contact when looking at the conversation partner's face displayed on the screen. This is due to the location disparity between the video conferencing window and the camera. This issue has been tackled by expensive high-end systems or hybrid depth+color cameras, but such equipment is still largely unavailable at the consumer level and on platforms such as laptops or tablets. In contrast, we propose a gaze correction method that needs just a single webcam. We apply recent shape deformation techniques to generate a 3D face model that matches the user's face. We then render a gaze-corrected version of this face model and seamlessly insert it into the original image. Experiments on real data and various platforms confirm the validity of the approach and demonstrate that the visual quality of our results is at least equivalent to those obtained by state-of-the-art methods requiring additional equipment.
Glucose Transport in the Renal Proximal Tubule (1992)

The sections in this article are: General Characteristics Glucose Handling by the Whole Kidney Tubular Site of Reabsorption D-Glucose Handling by the Intact Proximal Tubule Current Understanding of the Mechanism of Glucose Reabsorption Components of the Reabsorptive Mechanism Energetics: Gradient Hypothesis Na+ Dependence of Luminal versus Basolateral D-glucose Transport Kinetics and Stoichiometry Systematics of D-Glucose Reabsorption at the Cellular and Tubular Levels Specificity Characteristics Abnormalities of Glucose Reabsorption in the Renal Tubule Renal Glycosuria Fanconi's Syndrome Specific Defects of D-Glucose Transport Molecular Characterization of the Components of the D-Glucose Reabsorptive Pathway Across the Renal Proximal Tubule The Luminal Na+-Dependent D-Glucose Carrier The Basolateral Na+-Independent D-Glucose Carrier Molecular Mechanism of Operation of the Na+-Dependent D-Glucose Transporter Conclusion
Re-evaluation of the derivatives of the half order Fermi integrals (1993)

The Fermi integrals of half orders are important in the simulation of semiconductor transport processes. Several of these integrals (−1/2, 1/2, 3/2, 5/2) have been recently retabulated since the 1938 study by McDougall and Stoner [Phil. Trans. Roy. Soc. A 237, 67 (1938)], but the derivatives were not re-evaluated. The original integral values were calculated without the aid of high speed computers by using approximate series evaluation and tabulations of exponentials and zeta functions. In addition, a discrepancy was found in the literature since the original study in 1938. The second derivative of F1/2 has been mistakenly represented as being proportional to a Fermi integral of another order. This article tabulates the half order Fermi integrals from −1/2 to 5/2 over the reduced energy range −5 to 20 in 0.25 increments. The first two derivatives of F−1/2 are also calculated by numerical integration and tabulated to aid in interpolation. It is shown that the second derivative of F1/2 is not proportional to another Fermi integral. A suitable interpolation scheme is proposed to calculate the values of the Fermi integrals of various order to high accuracy over the total reduced energy range.
Improvement of Fe/MgO Catalysts by Calcination for the Growth of Single- and Double-Walled Carbon Nanotubes (2005)

Calcination at 900-1000 degrees C for 8-12 h of an Fe/MgO catalyst prepared by impregnation was found to result in a uniform MgFe2O4/MgO solid solution that showed a successful settling of well-dispersed iron species into the MgO lattice. During methane reduction, many iron-containing particles with a diameter of about 4 nm were formed on the catalyst surface to provide numerous active sites for the growth of single- and double-walled carbon nanotubes. There was a significant improvement of the Fe/MgO catalyst that resulted in a high yield of impurity-free nanotubes. Using C2H4 cracking at 600 degrees C and transmission electron microscope observations, the Fe species distribution in the catalysts and microscope images of nanotube growth were described in detail. H2 reduction of the calcined Fe/MgO catalyst was found to cause the formation of iron layers on the catalyst surface, which resulted in the growth of only carbon layers. The results are useful for understanding changes in the metal species distribution in the catalysts and the nanotube growth mechanism, and they provide a simple method to improve Fe/MgO catalysts.