gebhart/patrepeval huggingface dataset:
Differential roles of heat shock protein 70 in the in vitro nuclear import of glucocorticoid receptor and simian virus 40 large tumor antigen. (1994)

Nuclear import of glucocorticoid receptors (GRs) was analyzed in vitro with digitonin-permeabilized cells (S. A. Adam, R. Sterne-Marr, and L. Gerace, J. Cell Biol. 111:807-816, 1990). Indirect immunofluorescence methods were used to monitor the transport of GRs from rat hepatoma and fibroblast cell cytosol into HeLa nuclei. In vitro nuclear import of GRs was shown to be hormone dependent and to require ATP and incubation at ambient temperatures (i.e., 30 degrees C). Hormone-dependent dissociation of GR-bound proteins, such as the 90-kDa heat shock protein, hsp90, is part of an activation process that is obligatory for the expression of the receptor's DNA-binding activity. Inhibition of in vitro GR activation by Na2MoO4 blocked hormone-dependent nuclear import, demonstrating that receptor activation is required for nuclear import. The addition to GR-containing cytosol of antiserum directed against the cytosolic 70-kDa heat shock protein, hsp70, while effective in blocking the nuclear import of simian virus 40 large tumor antigen (SV40 TAg), did not affect hormone-dependent nuclear import of endogenous, full-length GRs or an exogenously added truncated GR protein (i.e., XGR556) that lacks a hormone-binding domain but possesses a constitutively active nuclear localization signal sequence (NLS). Depletion of hsp70 from HeLa cell cytosol did not affect the nuclear import of exogenously added XGR556 but led to inhibition of SV40 TAg nuclear import. Thus, two closely related NLSs, one contained within GRs and the other contained within SV40 TAg, are distinguished by their differential requirements for hsp70 in vitro.
NEW GENERATION OF HEALTHY SNACK FOOD BY SUPERCRITICAL FLUID EXTRUSION (2009)

ABSTRACT A supercritical fluid extrusion (SCFX) process has been successfully developed for the production of a novel healthy snack containing 40–60 wt% protein with unique porous structure and texture. Supercritical carbon dioxide (SC-CO2) injection rate and product temperature at the die were found to be critical to control the expansion and texture of the final product. Maximum cross-sectional expansion was obtained at 0.3 wt% added SC-CO2, whereas more uniform internal structure was achieved at 0.7 wt% SC-CO2 level. As whey protein concentrate (80 wt%) concentration was increased from 52.8 to 78.2 wt% in the formulation, the cross-sectional expansion of baked and fried products increased by 65.8 and 44.4%, respectively. It was observed that lower viscosity of whey protein compared with starch decreased expansion but helped enhance further expansion during post-extrusion drying. The finding showed that an extrusion process at the temperature below protein denaturation temperature using SC-CO2 can help to prevent hard texture due to the thermosetting property of whey protein and to create a uniformly expanded structure. The textural properties of SCFX chips were comparable to commercial extruded or fried chip products. PRACTICAL APPLICATIONS The American snack market is one of fast-growing markets in the world as snacking becomes more popular. Because of the increasing concerns about health, there is also an increasing demand for new healthy snacks as an alternative for fried starch-based snacks with low nutrient density. This study shows the potential of supercritical fluid extrusion (SCFX) technology for healthy snack food production containing whey protein. SCFX chips had uniform cellular microstructure that cannot be obtained using conventional steam-based extrusion. As supercritical carbon dioxide can deliver certain flavors, an expanded snack not only with high nutrient density and unique texture but also with encapsulated flavors can be produced using the SCFX process and can be marketed as a novel healthy snack.
Base-Free Aerobic Oxidation of 5-Hydroxymethyl-furfural to 2,5-Furandicarboxylic Acid in Water Catalyzed by Functionalized Carbon Nanotube-Supported Au–Pd Alloy Nanoparticles (2014)

The aerobic oxidation of 5-hydroxymethylfurfural (HMF), a key platform compound in cellulose transformation, into 2,5-furandicarboxylic acid (FDCA), a promising renewable alternative to petroleum-derived terephthalic acid, is one of the most attractive reactions for establishing biomass-based sustainable chemical processes. Supported Au catalysts have shown encouraging performance for this reaction, but the need of an excess amount of base additives makes the process less green and less cost-effective. Here, we report a stable and efficient carbon nanotube (CNT)-supported Au–Pd alloy catalyst for the aerobic oxidation of HMF to FDCA in water without any bases. The functionalization of CNT surfaces is crucial for FDCA formation. We have clarified that the CNT containing more carbonyl/quinone and less carboxyl groups favors FDCA formation by enhancing the adsorption of the reactant and reaction intermediates. Significant synergistic effects exist between Au and Pd in the alloy for the base-free oxidation of HMF to FDCA through three tandem steps. The present work provides understanding of the support-enhanced adsorption effect and the alloying effect for supported Au-based bimetallic catalysts, and this knowledge may help develop efficient catalysts for the aerobic oxidation of relatively complicated organic compounds with different functional groups in water.
Light intensity dependence of open-circuit voltage of polymer:fullerene solar cells (2005)

The open-circuit voltage Voc of polymer:fullerene bulk heterojunction solar cells is investigated as a function of light intensity for different temperatures. Devices consisted of a blend of a poly(p-phenylene vinylene) derivative as the hole conductor and 6,6-phenyl C61-butyric acid methyl ester as the electron conductor. The observed photogenerated current and Voc are at variance with classical p–n junction-based models. The influence of light intensity and recombination strength on Voc is consistently explained by a model based on the notion that the quasi-Fermi levels are constant throughout the device, including both drift and diffusion of charge carriers.
HSP70 Binding Sites in the Tumor Suppressor Protein p53 (1997)

Mutations within conserved regions of the tumor suppressor protein, p53, result in oncogenic forms of the protein with altered tertiary structures. In most cases, the mutant p53 proteins are selectively recognized and bound by members of the HSP70 family of molecular chaperones, but the binding site(s) in p53 for these chaperones have not been clearly defined. We have screened a library of overlapping biotinylated peptides, spanning the entire human p53 sequence, for binding to the HSP70 proteins, Hsc70 and DnaK. We show that most of the high affinity binding sites for these proteins map to secondary structure elements, particularly beta-strands, in the hydrophobic core of the central DNA binding domain, where the majority of oncogenic p53 mutations are found. Although peptides corresponding to the C-terminal region of p53 also contain potential binding sites, p53 proteins with C-terminal deletions are capable of binding to Hsc70, indicating that this region is not required for complex formation. We propose that mutations in the p53 protein alter the tertiary structure of the central DNA binding domain, thus exposing high affinity HSP70 binding sites that are cryptic in the wild-type molecule.
Analysis/Synthesis filter bank design based on time domain aliasing cancellation (1986)

A single-sideband analysis/synthesis system is proposed which provides perfect reconstruction of a signal from a set of critically sampled analysis signals. The technique is developed in terms of a weighted overlap-add method of analysis/synthesis and allows overlap between adjacent time windows. This implies that time domain aliasing is introduced in the analysis; however, this aliasing is cancelled in the synthesis process, and the system can provide perfect reconstruction. Achieving perfect reconstruction places constraints on the time domain window shape which are equivalent to those placed on the frequency domain shape of analysis/synthesis channels used in recently proposed critically sampled systems based on frequency domain aliasing cancellation [7], [8]. In fact, a duality exists between the new technique and the frequency domain techniques of [7] and [8], The proposed technique is more efficient than frequency domain designs for a given number of analysis/synthesis channels, and can provide reasonably band-limited channel responses. The technique could be particularly useful in applications where critically sampled analysis/synthesis is desirable, e.g., coding.
Autonomous Ground Vehicles—Concepts and a Path to the Future (2012)

Autonomous vehicles promise numerous improvements to vehicular traffic: an increase in both highway capacity and traffic flow because of faster response times, less fuel consumption and pollution thanks to more foresighted driving, and hopefully fewer accidents thanks to collision avoidance systems. In addition, drivers can save time for more useful activities. In order for these vehicles to safely operate in everyday traffic or in harsh off-road environments, a multitude of problems in perception, navigation, and control have to be solved. This paper gives an overview of the most current trends in autonomous vehicles, highlighting the concepts common to most successful systems as well as their differences. It concludes with an outlook into the promising future of autonomous vehicles.
Eclipse Modeling Framework (2003)

The authoritative guide to the Eclipse Modeling Framework (EMF)--written by the lead EMF designers! Shows how EMF unifies three important technologies: Java, XML, and UML @BULLET= Provides a comprehensive overview of the EMF classes including a complete quick reference for all the classes and methods in the EMF 1.1 API. Includes examples of many common framework customizations and programming techniques.
Comparison of the FT-IR Mapping and Imaging Techniques Applied to Polymeric Systems (2000)

Two Fourier transform infrared (FT-IR) spectrometers with microspectroscopic capabilities—one equipped with a focal plane array (FPA) mercury-cadmium-telluride (MCT) detector (imaging) and the other a single-element MCT detector (mapping)—are compared. Two samples, a one-dimensional diffusion system and a two-dimensional phase-separated system, were studied with both analytical tools. Diffusion regions and concentration gradients were determined in the diffusion sample. Phase-specific chemical composition data were obtained in the phase-separated sample. The utility of each instrument for specific cases and their respective limitations are illustrated. It is shown that the FPA system has the ability to sample a large sample area with high spatial resolution in a short time without adverse diffraction effects. Because of this very rapid sampling, many dynamic processes can be studied which are otherwise poorly monitored by the point-by-point mapping technique. The spatial resolution achievable, the quality of data obtained [signal-to-noise ratio (SNR)], and temporal resolution between mapped areas are intimately connected in the mapping system. However, the imaging systems have a fixed spatial resolution (dependent on the optics and detector) and the SNR considerations determine temporal resolution. However, it was also shown that by aperturing to a localized region, the single-element detector system is able to arrive at phase concentration information more rapidly, with less cumbersome processing and with higher SNRs than is possible with the current FPA technology. The chemical information obtained from both instruments is, within a small experimental error, identical. This work demonstrates what can be accomplished with each analytical FT-IR instrument, and serves as a comparative study to demonstrate the value of each analytical tool in specific situations.
A remarkably simple perfluoroalkylation in the presence of an electron mediator (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA remarkably simple perfluoroalkylation in the presence of an electron mediatorTomoya Kitazume and Takanobu IkeyaCite this: J. Org. Chem. 1988, 53, 10, 2349–2350Publication Date (Print):May 1, 1988Publication History Published online1 May 2002Published inissue 1 May 1988https://pubs.acs.org/doi/10.1021/jo00245a040https://doi.org/10.1021/jo00245a040research-articleACS PublicationsRequest reuse permissionsArticle Views277Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Radiobiology of the acute radiation syndrome (2011)

ACUTE RADIATION SYNDROME OR ACUTE RADIATION SICKNESS IS CLASSICALLY SUBDIVIDED INTO THREE SUBSYNDROMES: the hematopoietic, gastrointestinal and neurovascular syndrome but many other tissues can be damaged. The time course and severity of clinical signs and symptoms are a function of the overall body volume irradiated, the inhomogeneity of dose exposure, the particle type, the absorbed dose and the dose rate. Classical pathophysiology explain the failure of each of these organs and the timing of appearance of their signs and symptoms due to radiation-induced cytocidal effects of a great number of parenchymal cells of hierarchically organized tissues. Contemporaneously, many other radiation-induced effects has been described and all of them may lead to tissue injury with their corresponding signs and symptoms that can be expressed after short or long period of time. Radiation-induced multi-organ involvement is thought to be due to radiation-induced systemic inflammatory response mediated by released pro-inflammatory cytokines.
Bench‐Top Fabrication of Hierarchically Structured High‐Surface‐Area Electrodes (2013)

Abstract Fabrication of hierarchical materials, with highly optimized features from the millimeter to the nanometer scale, is crucial for applications in diverse areas including biosensing, energy storage, photovoltaics, and tissue engineering. In the past, complex material architectures have been achieved using a combination of top‐down and bottom‐up fabrication approaches. A remaining challenge, however, is the rapid, inexpensive, and simple fabrication of such materials systems using bench‐top prototyping methods. To address this challenge, the properties of hierarchically structured electrodes are developed and investigated by combining three bench‐top techniques: top‐down electrode patterning using vinyl masks created by a computer‐aided design (CAD)‐driven cutter, thin film micro/nanostructuring using a shrinkable polymer substrate, and tunable electrodeposition of conductive materials. By combining these methods, controllable electrode arrays are created with features in three distinct length scales: 40 μm to 1 mm, 50 nm to 10 μm, and 20 nm to 2 μm. The electrical and electrochemical properties of these electrodes are analyzed and it is demonstrated that they are excellent candidates for next generation low‐cost electrochemical and electronic devices.
Selective anti-cancer agents as anti-aging drugs (2013)

Recent groundbreaking discoveries have revealed that IGF-1, Ras, MEK, AMPK, TSC1/2, FOXO, PI3K, mTOR, S6K, and NFκB are involved in the aging process. This is remarkable because the same signaling molecules, oncoproteins and tumor suppressors, are well-known targets for cancer therapy. Furthermore, anti-cancer drugs aimed at some of these targets have been already developed. This arsenal could be potentially employed for anti-aging interventions (given that similar signaling molecules are involved in both cancer and aging). In cancer, intrinsic and acquired resistance, tumor heterogeneity, adaptation, and genetic instability of cancer cells all hinder cancer-directed therapy. But for anti-aging applications, these hurdles are irrelevant. For example, since anti-aging interventions should be aimed at normal postmitotic cells, no selection for resistance is expected. At low doses, certain agents may decelerate aging and age-related diseases. Importantly, deceleration of aging can in turn postpone cancer, which is an age-related disease.
Compensation of eddy-current-induced magnetic field transients in a MOT (2007)

The design and implementation of a current driver for the quadrupole coil of a magneto-optical trap are presented. The control and the power stages of the driver, both based on a push-pull configuration with high-speed transistors, are separated, allowing for a protection of the control electronics. Moreover, the coil current can be set by an analog voltage, a feature that makes possible both fast and adiabatic switching of the quadrupole magnetic field. In order to compensate magnetic field transients induced by eddy currents, the driver allows a quick reversal of the quadrupole coil current providing in such a way the required magnetic flux compensation. From a fit to the measured magnetic field transients, we have extracted the decay constants which confirm the efficiency of the compensation. Furthermore, we measured the influence of eddy currents on a sample of cold caesium atoms, testing at the same time the ultimate performance of the driver for our applications. The results show that the implemented electronic circuit is able to reduce substantially the transient effects with switching times of the magnetic field below 100 microseconds.
Online Dynamic Reordering for Interactive Data Processing (1999)

Abstract We present a pipelining, dynamically user-controllable reorder operator, for use in data-intensive applications. Allowing the user to reorder the data delivery on the fly increases the interactivity in several contexts such as online aggregation and large-scale spreadsheets; it allows the user to control the processing of data by dynamically specifying preferences for different data items based on prior feedback, so that data of interest is prioritized for early processing.
Temperature Dependent Dielectric Properties of Ba(Zn[sub 1∕3]Ta[sub 2∕3])O[sub 3] Ceramics (2011)

BZT ceramics were prepared by solid state reaction method. Processing parameters such as calcination temperature, sintering temperature and sintering durations were optimized to get best dielectric properties. It was found that the BZT ceramics sintered at 1500 °C for 3 hours exhibited maximum density and uniform microstructure. Dielectric properties of BZT ceramics were measured from room temperature to 500 °C. It was observed that as the temperature increases both the dielectric constant and loss increases and dielectric constant and dielectric loss found to be in the range of 13.5–35 and 7×10−5 to 4×10−2 respectively. The effect of processing parameters, density and microstructure on dielectric properties of BZT ceramics studied and will be presented.
Augmenting spatial awareness with Haptic Radar (2006)

We are developing a modular electronic device to allow users to perceive and respond simultaneously to multiple spatial information sources using haptic stimulus. Each module of this wearable "haptic radar" acts as an artificial hair capable of sensing obstacles, measuring their range and transducing this information as a vibro-tactile cue on the skin directly beneath the module. Our first prototype (a headband) provides the wearer with 360 degrees of spatial awareness thanks to invisible, insect-like antennas. During a proof-of-principle experiment, a significant proportion (87%, p = 1.26 <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">*</sub> 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-5</sup> ) of participants moved to avoid an unseen object approaching from behind without any previous training. Participants reported the system as more of a help, easy, and intuitive. Among the numerous potential applications of this interface are electronic travel aids and visual prosthetics for the blind, augmentation of spatial awareness in hazardous working environments, as well as enhanced obstacle awareness for motorcycle or car drivers (in this case the sensors may cover the surface of the car).
Translational control by 5′-untranslated regions of eukaryotic mRNAs (2016)

The eukaryotic 5' untranslated region (UTR) is critical for ribosome recruitment to the messenger RNA (mRNA) and start codon choice and plays a major role in the control of translation efficiency and shaping the cellular proteome. The ribosomal initiation complex is assembled on the mRNA via a cap-dependent or cap-independent mechanism. We describe various mechanisms controlling ribosome scanning and initiation codon selection by 5' upstream open reading frames, translation initiation factors, and primary and secondary structures of the 5'UTR, including particular sequence motifs. We also discuss translational control via phosphorylation of eukaryotic initiation factor 2, which is implicated in learning and memory, neurodegenerative diseases, and cancer.
Effect of signal intensity and camera quantization on laser speckle contrast analysis (2012)

Laser speckle contrast analysis (LASCA) is limited to being a qualitative method for the measurement of blood flow and tissue perfusion as it is sensitive to the measurement configuration. The signal intensity is one of the parameters that can affect the contrast values due to the quantization of the signals by the camera and analog-to-digital converter (ADC). In this paper we deduce the theoretical relationship between signal intensity and contrast values based on the probability density function (PDF) of the speckle pattern and simplify it to a rational function. A simple method to correct this contrast error is suggested. The experimental results demonstrate that this relationship can effectively compensate the bias in contrast values induced by the quantized signal intensity and correct for bias induced by signal intensity variations across the field of view.
HSF1 granules: a novel stress-induced nuclear compartment of human cells (1997)

Heat shock factor 1 (HSF1) is the ubiquitous stress-responsive transcriptional activator which is essential for the inducible transcription of genes encoding heat shock proteins and molecular chaperones. HSF1 localizes within the nucleus of cells exposed to heat shock, heavy metals, and amino acid analogues, to form large, irregularly shaped, brightly staining granules which are not detected during attenuation of the heat shock response or when cells are returned to their normal growth conditions. The kinetics of detection of HSF1 granules parallels the transient induction of heat shock gene transcription. HSF1 granules are also detected using an HSF1-Flag epitope tagged protein or a chimeric HSF1-green fluorescent protein which reveals that these nuclear structures are stress-induced and can be detected in living cells. The spatial organization of HSF1 granules in nuclei of stressed cells reveals that they are novel nuclear structures which are stress-dependent and provides evidence that the nucleus undergoes dynamic reorganization in response to stress.