gebhart/patrepeval huggingface dataset:
Direct Visualization of the Translocation of the γ-Subspecies of Protein Kinase C in Living Cells Using Fusion Proteins with Green Fluorescent Protein (1997)

We expressed the gamma-subspecies of protein kinase C (gamma-PKC) fused with green fluorescent protein (GFP) in various cell lines and observed the movement of this fusion protein in living cells under a confocal laser scanning fluorescent microscope. gamma-PKC-GFP fusion protein had enzymological properties very similar to that of native gamma-PKC. The fluorescence of gamma-PKC- GFP was observed throughout the cytoplasm in transiently transfected COS-7 cells. Stimulation by an active phorbol ester (12-O-tetradecanoylphorbol 13-acetate [TPA]) but not by an inactive phorbol ester (4alpha-phorbol 12, 13-didecanoate) induced a significant translocation of gamma-PKC-GFP from cytoplasm to the plasma membrane. A23187, a Ca2+ ionophore, induced a more rapid translocation of gamma-PKC-GFP than TPA. The A23187-induced translocation was abolished by elimination of extracellular and intracellular Ca2+. TPA- induced translocation of gamma-PKC-GFP was unidirected, while Ca2+ ionophore-induced translocation was reversible; that is, gamma-PKC-GFP translocated to the membrane returned to the cytosol and finally accumulated as patchy dots on the plasma membrane. To investigate the significance of C1 and C2 domains of gamma-PKC in translocation, we expressed mutant gamma-PKC-GFP fusion protein in which the two cysteine rich regions in the C1 region were disrupted (designated as BS 238) or the C2 region was deleted (BS 239). BS 238 mutant was translocated by Ca2+ ionophore but not by TPA. In contrast, BS 239 mutant was translocated by TPA but not by Ca2+ ionophore. To examine the translocation of gamma-PKC-GFP under physiological conditions, we expressed it in NG-108 cells, N-methyl-D-aspartate (NMDA) receptor-transfected COS-7 cells, or CHO cells expressing metabotropic glutamate receptor 1 (CHO/mGluR1 cells). In NG-108 cells , K+ depolarization induced rapid translocation of gamma-PKC-GFP. In NMDA receptor-transfected COS-7 cells, application of NMDA plus glycine also translocated gamma-PKC-GFP. Furthermore, rapid translocation and sequential retranslocation of gamma-PKC-GFP were observed in CHO/ mGluR1 cells on stimulation with the receptor. Neither cytochalasin D nor colchicine affected the translocation of gamma-PKC-GFP, indicating that translocation of gamma-PKC was independent of actin and microtubule. gamma-PKC-GFP fusion protein is a useful tool for investigating the molecular mechanism of gamma-PKC translocation and the role of gamma-PKC in the central nervous system.
\nQuantifying the Encapsulation of Implemented Software Architectures (2014)

\n Contains fulltext :\n 132652.pdf (Publisher’s version ) (Closed access)\n
Circulating RNA Transcripts Identify Therapeutic Response in Cystic Fibrosis Lung Disease (2008)

Circulating mononuclear cell transcripts characterize a response to the treatment of pulmonary exacerbations. Even in small patient cohorts, changes in gene expression in conjunction with FEV(1) may enhance current outcomes measures for treatment response.
Comparison of carbonate, citrate, and oxalate chemical routes to the high-Tc metal oxide superconductors, lanthanum strontium copper oxide, La2-xSrxCuO4 (1987)

補正完畢
RMM: a methodology for structured hypermedia design (1995)

Hypermedia projects differ from traditional software development projects in several critical dimensions. First, as Streitz notes in this issue, hypermedia projects may involve people with very different skill sets: authors, librarians, content designers, artists, and musicians, as well as programmers. Second, the design of hypermedia applications involves capturing and organizing the structure of a complex domain and making it clear and accessible to users Third, multimedia aspects of hypermedia applications raise numerous difficulties Hypermedia design is therefore a challenging process that is currently more of an art than a science. Finally, the need for prototyping (see Nanard and Nanard in this issue) and intensive testing with users is even more pronounced in hypermedia development than it is with traditional software, because user tolerance to errors in hypermedia applications is very low.
The Mammalian Cytosolic Selenoenzyme Thioredoxin Reductase Reduces Ubiquinone (2003)

The selenoprotein thioredoxin reductase (TrxR1) is an essential antioxidant enzyme known to reduce many compounds in addition to thioredoxin, its principle protein substrate. Here we found that TrxR1 reduced ubiquinone-10 and thereby regenerated the antioxidant ubiquinol-10 (Q10), which is important for protection against lipid and protein peroxidation. The reduction was time- and dose-dependent, with an apparent K(m) of 22 microm and a maximal rate of about 12 nmol of reduced Q10 per milligram of TrxR1 per minute. TrxR1 reduced ubiquinone maximally at a physiological pH of 7.5 at similar rates using either NADPH or NADH as cofactors. The reduction of Q10 by mammalian TrxR1 was selenium dependent as revealed by comparison with Escherichia coli TrxR or selenium-deprived mutant and truncated mammalian TrxR forms. In addition, the rate of reduction of ubiquinone was significantly higher in homogenates from human embryo kidney 293 cells stably overexpressing thioredoxin reductase and was induced along with increasing cytosolic TrxR activity after the addition of selenite to the culture medium. These data demonstrate that the selenoenzyme thioredoxin reductase is an important selenium-dependent ubiquinone reductase and can explain how selenium and ubiquinone, by a combined action, may protect the cell from oxidative damage.
Affinity-column-mediated immunoenzymometric assays: influence of affinity-column ligand and valency of antibody-enzyme conjugates. (1984)

We describe an affinity-column-mediated, enzyme-linked immunometric assay that is highly sensitive and adaptable to automation. Digoxin is the model test analyte. A comparison of digoxin with its analog, ouabain, for use as the immobilized ligand on the affinity column showed ouabain to be superior. We also report the effect of column elution rate. Antibody-enzyme conjugates prepared with the monovalent Fab'-fragment and the divalent F(ab')2-fragment coupled to beta-galactosidase are compared in terms of their overall assay performance. Although the monovalent Fab'--beta-galactosidase conjugate yields a more sensitive assay and dose-response curves that are linear over a wider range, the divalent F(ab')2--beta-galactosidase conjugate provides an assay with adequate sensitivity and extremely good precision, and is generally easier to synthesize reproducibly. This fully automated, rapid, and precise assay for digoxin is compatible with the Du Pont aca discrete clinical analyzer.
Biochemistry and clinical pharmacology of new anticoagulant agents (2002)

New anticoagulants have been designed using advances in the biotechnology and the biochemistry of the clotting factors. They are classified according to their target or their mechanism of action (AT-dependent or AT-independent). Antithrombotic activity after oral administration is another important property. This abstract is restricted to the synthetic pentasaccharide and the ximelagatran/melagatran. Fondaparinux (Arixtra) is a chemically synthesized methoxy derivative of the natural pentasaccharide (MW=1728 Da). Fondaparinux has nearly complete bioavailabity after subcutaneous injection. The elimination half-life is about 17 hours. Fondaparinux is cleared from the kidneys. In contrast to heparin, fondaparinux does not interact with plasma proteins other than antithrombin and it does not cross-react in vitro with heparin induced thrombocytopenia antibodies. Fondaparinux inhibits specifically FXa, although some other targets are suspected (FIXa and FVIIa). Endogenous levels of AT are the rate limiting factor for its anti-Xa activity. Fondaparinux, in contrast to direct FXa inhibitors (i.e. DX-9065a) inhibits free FXa but not the FXa bound to prothrombinase, and it does not prolong PT and aPTT. Fondaparinux inhibits thrombin generation in human plasma. We have shown that the inhibition of thrombin generation by fondaparinux is inversely correlated with thromboplastin concentration. This original observation may explain the absence of any effect of fondaparinux on PT. Melagatran dipeptide (MW=430 Da) is a specific, reversible direct thrombin inhibitor. It inhibits free and clot bound thrombin. Its half-life is 1.7-2.5 hours after i.v. or s.c. administration. Ximelagatran (Exantas), is a pro-drug with a hydroxyamidino instead of amidino-group and an esterified carboxyl-group. It is the first clinically used direct orally acting thrombin inhibitor. Ximelagatran after intestinal absorption is metabolized to melagatran. Melagatran is not metabolized and is cleared through the kidney. Among the specific FXa inhibitors the synthetic pentasaccharide (Fondaparinux-Arixtra) has been approved by FDA and several European health organizations for the prophylaxis of VTE in major orthopedic surgery. No specific antidote has been described for the new antithrombotics. Encouraging results have been obtained from our group on the possible use of rFVIIa (Novoseven). The general opinion is that there is no need for laboratory monitoring of the new antithrombotic agents. Conclusion. The research for safer and more effective anticoagulants has been successful by targeting specific steps in coagulation. These single-targeted agents are challenging multi-targeted drugs (heparins and vitamin-K antagonists). Other drugs including orally active FXa direct inhibitors will probably enrich the armentarium of antithrombotic drugs.
Shedding Old Paradigms: Developing Viruses to Treat Cancer (2002)

Article Tools EDITORIALS Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2002.20.9.2220 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 11980991 Shedding Old Paradigms: Developing Viruses to Treat Cancer Emily K. BergslandxEmily K. BergslandSearch for articles by this author , Alan P. VenookxAlan P. VenookSearch for articles by this author Show More University of California, San Francisco, CA https://doi.org/10.1200/JCO.2002.20.9.2220 First Page Full Text PDF Figures and Tables © 2002 by American Society of Clinical Oncology
Ketamine-Associated Corneal Edema (2012)

Ketamine may induce potentially reversible corneal edema with endothelial cell loss. A thorough medication history of this and other drugs should be sought in patients with an otherwise unexplained corneal endothelial failure.
El principio protector y la regla del in dubio pro operario como criterio de interpretación de la norma laboral (2016)

The article consists in the review of the protection principie and its manifestation in the in dubio pro operario, from a foreign, especially continental European law, and Chilean law perspective. It is possible to conclude that the in dubio pro operario is nota principie ofLabor Law but only an interpretation criteria. The in dubio pro operario is not considered by the current European doctrine and jurisprudence. Even if estimated possible it should be applied in a very limited way. Its application could create legal uncertainty. It could never be used to fill a legal vacuum or to create situations not considered by the law.
Pathogenesis of familial periodic fever syndromes or hereditary autoinflammatory syndromes (2006)

Familial periodic fever syndromes, otherwise known as hereditary autoinflammatory syndromes, are inherited disorders characterized by recurrent episodes of fever and inflammation. The general hypothesis is that the innate immune response in these patients is wrongly tuned, being either too sensitive to very minor stimuli or turned off too late. The genetic background of the major familial periodic fever syndromes has been unraveled, and through research into the pathophysiology, a clearer picture of the innate immune system is emerging. After an introduction on fever, interleukin-1beta and inflammasomes, which are involved in the majority of these diseases, this manuscript offers a detailed review of the pathophysiology of the cryopyrin-associated periodic syndromes, familial Mediterranean fever, the syndrome of pyogenic arthritis, pyoderma gangrenosum and acne, Blau syndrome, TNF-receptor-associated periodic syndrome and hyper-IgD and periodic fever syndrome. Despite recent major advances, there are still many questions to be answered regarding the pathogenesis of these disorders.
Leukotriene B<sub>4</sub>Receptor (BLT-1) Modulates Neutrophil Influx into the Peritoneum but Not the Lung and Liver during Surgically Induced Bacterial Peritonitis in Mice (2004)

Leukotriene B4 (LTB4) is a rapidly synthesized, early neutrophil chemoattractant that signals via its cell surface receptor, BLT-1, to attract and activate neutrophils during peritonitis. BLT-1-deficient (BLT-1(-/-)) mice were used to determine the effects of LTB4 on neutrophil migration and activation, bacterial levels, and survival after cecal ligation and puncture (CLP). Male BLT-1(-/-) or wild-type (WT) BALB/c mice underwent CLP. Tissues were harvested for determination of levels of bacteria, myeloperoxidase (MPO), LTB4, macrophage inflammatory protein 2 (MIP-2), and neutrophil (polymorphonuclear leukocyte [PMN]) numbers at 4 and 18 h after CLP. PMN activation was determined by an assessment of phagocytosis ability and CD11b expression. Survival was also determined. BLT-1(-/-) mice had decreased numbers of PMNs in the peritoneum at both 4 and 18 h after CLP but increased numbers of PMNs in the blood at 18 h compared with WT mice. Liver and lung MPO levels were significantly higher in BLT-1(-/-) mice at both 4 and 18 h after CLP, with increased bacterial levels in the blood, the liver, and peritoneal fluid at 4 h. Bacterial levels remained higher in peritoneal fluid at 18 h, but blood and liver bacterial levels at 18 h were not different from levels at 4 h. PMN phagocytosis and CD11b levels were decreased in BLT-1(-/-) mice. LTB4 levels were similar between the groups before and after CLP, but MIP-2 levels were decreased both locally and systemically in BLT-1(-/-) mice. Survival was significantly improved in BLT-1(-/-) mice (71%) compared with WT mice (14%) at 48 h post-CLP. Thus, LTB4 modulates neutrophil migration into the mouse peritoneum, but not the lung or liver, after CLP. Despite higher bacterial and PMN levels at remote sites, there was increased survival in BLT-1(-/-) mice compared to WT mice. Decreased PMN activation may result in less remote organ dysfunction and improved survival.
Use of Cell-Free Circulating Schistosome DNA in Serum, Urine, Semen, and Saliva To Monitor a Case of Refractory Imported Schistosomiasis Hematobia (2013)

This case of imported refractory schistosomiasis has highlighted the usefulness of cell-free parasite DNA as a diagnostic marker to assess active schistosome infection. In contrast to the rapid disappearance of ova in urine, parasite DNA remained persistent in several other specimen types even after the fourth treatment with praziquantel. This result was consistent with the presence of morphologically intact ova in bladder biopsy samples and with the corresponding symptoms.
c-myc/p53 interaction determines sensitivity of human colon carcinoma cells to 5-fluorouracil in vitro and in vivo. (2001)

Colon carcinoma cells overexpress c-myc due to defective Wnt signaling, but only patients whose tumors have an amplified c-myc gene show improved disease-free and overall survival in response to 5-fluoruracil (5FU). Here we show that in two colon carcinoma cell lines that do not have an amplified c-myc gene but differ in their p53 status, high c-myc levels can be further elevated by introducing a c-myc expression vector. Whereas sensitivity to low serum-induced apoptosis was imposed on the parental lines independent of p53 status and was unaffected by further elevation of c-myc, sensitivity to 5FU-induced apoptosis was dependent on both the higher c-myc levels due to the expression vector and wild-type p53 function. The elevated c-myc levels led to higher c-myc transactivation activity in the p53 wild-type cell line, but not in the mutant p53 cell line. The requirement for both elevated c-myc and p53 for 5FU sensitivity was confirmed using antisense c-myc and pifithrin-alpha, a specific inhibitor of p53. Finally, the in vitro data predicted that only patients with both amplified c-myc and wild-type p53 in their primary tumors would be responsive to 5FU-based therapy, which was borne out by analysis of tumors from 135 patients entered into a Phase III clinical trial of 5FU-based adjuvant therapy. The data provide significant insight into mechanisms that establish colon tumor cell sensitivity to 5FU, clearly demonstrate the necessity of exercising caution in considering combining novel strategies that target elevated c-myc with standard 5FU-based therapy, and suggest alternative therapeutic strategies that target c-myc and/or p53 mutations in the treatment of colon cancer.
[Effect of low-energy laser irradiation of bronchial mucosa on systemic and local immunity in patients with chronic bronchitis]. (1991)

The effectiveness of endobronchial low-energy laser therapy was studied in 28 patients with chronic nonobstructive bronchitis concurrent with thinning of bronchial mucosa. The course of treatment made it possible to obtain positive dynamics of most parameters of immunologic reactivity in CNB patients. Systemic immunity parameters, except for the NST and concentration of circulating immune complexes, underwent essential normalization. Marked positive changes were found in the parameters characterizing functional activity of alveolar macrophages: there was a 2.5-fold increase in adhesive properties and over 1.3-fold increase in the percentage of phagocytes. The level of secretory IgA rose significantly (by 3.5 times). Hence, a manifested therapeutic effect of this therapeutic method is mainly associated with its immune-stimulating action.
Adaptive stochastic approximation by the simultaneous perturbation method (2000)

Stochastic approximation (SA) has long been applied for problems of minimizing loss functions or root finding with noisy input information. As with all stochastic search algorithms, there are adjustable algorithm coefficients that must be specified, and that can have a profound effect on algorithm performance. It is known that choosing these coefficients according to an SA analog of the deterministic Newton-Raphson algorithm provides an optimal or near-optimal form of the algorithm. However, directly determining the required Hessian matrix (or Jacobian matrix for root finding) to achieve this algorithm form has often been difficult or impossible in practice. The paper presents a general adaptive SA algorithm that is based on a simple method for estimating the Hessian matrix, while concurrently estimating the primary parameters of interest. The approach applies in both the gradient-free optimization (Kiefer-Wolfowitz) and root-finding/stochastic gradient-based (Robbins-Monro) settings, and is based on the "simultaneous perturbation (SP)" idea introduced previously. The algorithm requires only a small number of loss function or gradient measurements per iteration-independent of the problem dimension-to adaptively estimate the Hessian and parameters of primary interest. Aside from introducing the adaptive SP approach, the paper presents practical implementation guidance, asymptotic theory, and a nontrivial numerical evaluation. Also included is a discussion and numerical analysis comparing the adaptive SP approach with the iterate-averaging approach to accelerated SA.
3D video surveillance with Augmented Virtual Environments (2003)

Recent advances in sensing and computing technologies have inspired a new generation of data analysis and visualization systems for video surveillance applications. We present a novel visualization system for video surveillance based on an Augmented Virtual Environment (AVE) that fuses dynamic imagery with 3D models in a real-time display to help observers comprehend multiple streams of temporal data and imagery from arbitrary views of the scene. This paper focuses on our recent technical extensions to our AVE system, including moving object detection, tracking, and 3D display for effective dynamic event comprehension and situational awareness. Moving objects are detected and tracked in video sequences and visualized as pseudo-3D elements in the AVE scene display in real-time. We show results that illustrate the utility and benefits of these new capabilities.
Possibility of Terahertz Observations at the ALMA site (2011)

Observational rates under terahertz (THz) opacities less than 3.0 and 2.0 at the Atacama Large Millimeter/submillimeter Array (ALMA) site have been calculated using the 225 GHz tipping radiometer monitoring data and the opacity correlation between 225 GHz and THz opacities. The observational rate with THz opacity condition less than 3.0 is 12.4% in a year, and in winter (November - April) it is about twice higher than in summer (May - October). This observational rate shows a large sinusoidal annual variation, and it seems to have relation with the El Niño and La Niña phenomena; the La Niña years tend to have high observational rates, but the El Niño years show low rates. On the other hand, the observational rate with the THz opacity condition less than 2.0 is only 1.9%, and no obvious annual and seasonal variations are observed. This indicates that THz observations under low opacity condition of less than 2.0 at the ALMA site are very difficult to be performed.
AAV retinal transduction in a large animal model species: comparison of a self-complementary AAV2/5 with a single-stranded AAV2/5 vector. (2009)

When delivered by subretinal injection in the dog, scAAV2/5 induces faster and stronger transgene expression than ssAAV2/5. The spectrum of retinal neurons transduced is similar between the two vectors. These results confirm in a large animal model those previously reported in the mouse. ScAAV2/5 shows promise for use in the treatment of conditions where a rapid transgene expression is desirable. Furthermore, it may be possible to use a lower number of viral particles to achieve the same effect compared with ssAAV2/5 vectors.
Chemistry, isolation and purification of gastrointestinal hormones (1980)

Conference Article| February 01 1980 Chemistry, isolation and purification of gastrointestinal hormones VIKTOR MUTT VIKTOR MUTT 1Department of Biochemistry II, Karolinska Institute, S-104 01 Stockholm, Sweden Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1980) 8 (1): 11–14. https://doi.org/10.1042/bst0080011 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation VIKTOR MUTT; Chemistry, isolation and purification of gastrointestinal hormones. Biochem Soc Trans 1 February 1980; 8 (1): 11–14. doi: https://doi.org/10.1042/bst0080011 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1980 Biochemical Society1980 Article PDF first page preview Close Modal You do not currently have access to this content.