gebhart/patrepeval huggingface dataset:
Comparison of in vitro and in vivo mitogenic and polyclonal antibody and autoantibody responses to peptidoglycan, LPS, protein A, PWM, PHA and Con A in normal and autoimmune mice. (1985)

We have compared the in vitro and in vivo mitogenic and polyclonal antibody (IgM-, IgG-, IgA- and IgM anti-SRBC-secreting PFC) and autoantibody (IgM anti-ssDNA-, anti-bromelin-treated [HB]- and anti-intact mouse RBC-secreting PFC) responses to peptidoglycan (PG), LPS, protein A, PWM, PHA and Con A in young (4-7 weeks) and old (7-8 months) normal (BALB/c, CBA/H, C57BL/6) and autoimmune (NZB, NZB X NZW F1, BXSB, MRL/1; old BXSB and MRL/1 were 4-5 months) mice. Our results demonstrated that: lymphocytes from young and old autoimmune mice (except old BXSB) could be further polyclonally activated in vitro by PG or LPS as well as or better than lymphocytes from young and old normal mice; lymphocytes from young or old autoimmune mice were less polyclonally activated in vitro by protein A or PWM, respectively, than lymphocytes from young or old normal mice; PG and LPS were equally effective polyclonal activators in vitro; in vivo, LPS was a stronger stimulant than PG; in vivo, LPS could induce polyclonal activation in both young and old normal and autoimmune mice, whereas, PG could only induce polyclonal activation in vivo in young and old normal mice, but did not induce further activation in young and old autoimmune mice; only some tests (anti-ssDNA and IgG PFC in vivo, and IgA and anti-HB MRBC PFC in vitro) revealed higher responses in autoimmune than in normal mice, and these higher responses were seen more often in vivo than in vitro; both autoimmune and normal mice had a high frequency of autoantibody (especially anti-ssDNA) secreting cells in polyclonal activation in vitro, whereas a high frequency of these cells in vivo was only found in autoimmune mice; in most cases in vitro, polyclonal activators did not change the frequency of autoantibody and heteroantibody secreting cells, but in vivo, both PG and LPS increased the frequency of anti-ssDNA antibody secreting cells in normal, but not in autoimmune, mice; LPS increased the in vivo, but not in vitro, frequency of cells secreting anti-HB MRBC antibodies in some strains of mice; old mice had lower mitogenic responsiveness than young mice in both autoimmune and normal strains; autoimmune mice had similar, higher or lower mitogenic responses than normal mice, depending on the strain and the age, but in most cases consistent for both B and T cell mitogens; and there was no correlation between the patterns of increased or decreased mitogenic and polyclonal antibody responses in normal and autoimmune mice.4+.
Transplantation of skeletal myoblasts secreting an IL-1 inhibitor modulates adverse remodeling in infarcted murine myocardium (2004)

After myocardial infarction (MI), adverse remodeling with left ventricular (LV) dilatation is a major determinant of poor outcome. Skeletal myoblast (SkM) implantation improves cardiac function post-MI, although the mechanism is unclear. IL-1 influences post-MI hypertrophy and collagen turnover and is implicated in SkM death after grafting. We hypothesized that SkM expressing secretory IL-1 receptor antagonist (sIL-1ra) at MI border zones would specifically attenuate adverse remodeling and exhibit improved graft cell number. Stable murine male SkM lines (5 x 10(5) cells), expressing or nonexpressing (cont) for sIL-1ra, were implanted into infarct border zones of female nude mice immediately after left coronary artery occlusion. LV ejection fraction (LVEF), end-diastolic diameter, and transmitral peak early/late (E/A) flow velocity ratio were determined by echocardiography. Cardiac myocyte hypertrophy and fibrosis were assessed by morphometry, picrosirius red staining, and hydroxyproline assay. At 3 weeks, cont-SkM-engrafted hearts showed reduced hypertrophy, improved LVEF (55.7 +/- 1.2% vs. MI-only: 40.3 +/- 2.9%), and preserved E/A ratios. sIL-1ra-SkM implantation enhanced these effects (LVEF, 67.0 +/- 2.3%) and significantly attenuated LV dilatation (LV end-diastolic diameter, 4.0 +/- 1.1 mm vs. cont-SkM, 4.5 +/- 1.2 mm vs. MI-only, 4.8 +/- 1.8 mm); this was associated with greater graft numbers, as shown by PCR for male-specific smcy gene. Enzyme zymography showed attenuated matrix metalloproteinase-2 and -9 up-regulation post-MI by either donor SkM type, although infarct-remote zone collagen was reduced only with sIL-1ra-SkM. These results suggest that SkM implantation improves cardiac function post-MI by modulation of adverse remodeling, and that this effect can be significantly enhanced by targeting IL-1 as a key upstream regulator of both adverse remodeling and graft cell death.
Chromosomal Region of the Cystic Fibrosis Gene in Yeast Artificial Chromosomes: A Model for Human Genome Mapping (1990)

A general strategy for cloning and mapping large regions of human DNA with yeast artificial chromosomes (YAC's) is described. It relies on the use of the polymerase chain reaction to detect DNA landmarks called sequence-tagged sites (STS's) within YAC clones. The method was applied to the region of human chromosome 7 containing the cystic fibrosis (CF) gene. Thirty YAC clones from this region were analyzed, and a contig map that spans more than 1,500,000 base pairs was assembled. Individual YAC's as large as 790 kilobase pairs and containing the entire CF gene were constructed in vivo by meiotic recombination in yeast between pairs of overlapping YAC's.
Wax ester fermentation in <i>Euglena gracilis</i> (1982)

Aerobically grown Euglena gracilis , a bleached mutant, shows a prompt synthesis of wax esters with the concomitant fall of the paramylon (a β‐1,3‐glucan) content upon exposure to anaerobiosis. Bringing the anaerobic cells back to aerobiosis causes the reverse conversions. The anaerobic wax ester formation is accompanied by a net synthesis of ATP. The transition between the fermentation and respiration occurs at 10 −5 –10 −7 M of the O 2 concentration.
Tomato Chlorosis Virus: A New Whitefly-Transmitted, Phloem-Limited, Bipartite Closterovirus of Tomato (1998)

ABSTRACT Tomato chlorosis virus (ToCV) is the second whitefly-transmitted, phloem-limited, bipartite closterovirus described infecting tomato. ToCV is distinct from tomato infectious chlorosis virus (TICV), based on lack of serological and nucleic acid cross-reactions and differences in vector specificity. TICV is transmitted only by the greenhouse whitefly (Trialeurodes vaporariorum), whereas ToCV is transmitted by the greenhouse whitefly, the banded-wing whitefly (T. abutilonea), and Bemisia tabaci biotypes A and B (B. argentifolii). Double-stranded (ds) RNA analyses of ToCV show two prominent dsRNAs of approximately 7,800 and 8,200 bp, with several small dsRNAs. Digoxigenin-11-UTP-labeled riboprobes derived from cDNA clones representing portions of RNAs 1 and 2 were used in Northern blot hybridizations to detect two large nonhomologous dsRNAs and a subset of smaller dsRNAs. These probes were used in dot blot hybridizations to detect ToCV in infected tomato. Inclusion bodies and cytoplasmic vesicles were consistently observed in phloem tissues of ToCV-infected Nicotiana clevelandii. Computer-assisted sequence analysis showed significant homology between ToCV clones that hybridize specifically with RNAs 1 and 2 and the lettuce infectious yellows virus methyltransferase of RNA 1 and the HSP70 heat shock protein homolog of RNA 2, respectively. Thus, ToCV is another member of the growing subgroup of bipartite closteroviruses transmitted by whiteflies.
Experimental Vaccine Protection Against Feline Immunodeficiency Virus (1991)

Infection of domestic cats with the feline immunodeficiency virus (FIV) represents an important veterinary health problem and a useful animal model for the development of vaccines against acquired immunodeficiency syndrome (AIDS). Two experimental FIV vaccines have been developed; one consisting of fixed infected cells (Vaccine 1), the other of inactivated whole virus (Vaccine 2). After 4-6 immunizations over 2-5 months, both vaccines induced a strong FIV-specific immune response including neutralizing antibody and T-cell proliferation. Vaccine 1 protected 6 of 9 and Vaccine 2 protected 5 of 6 recipient cats against any detectable infection with a low dose (10 animal ID50) of FIV given intraperitoneally 2 weeks after the final boost. One additional cat in each vaccine group had a transient infection at 5-7 weeks postchallenge following which virus could no longer be detected. Thus, a total of 13 of 15 vaccinated cats were protected against persistent infection. By contrast, 13 of 13 controls were persistently infected by this challenge. The infected cell vaccine failed to protect against a higher dose (5 x 10(4) ID50) of FIV. These results indicate that vaccine prophylaxis against natural FIV infection should be achievable and enhance optimism of the prospect of developing an effective AIDS vaccine for humans.
The influence of <i>CYP2D6</i> genotype on trough plasma perhexiline and <i>cis</i>‐OH‐perhexiline concentrations following a standard loading regimen in patients with myocardial ischaemia (2005)

Patients with only one functional allele and not CYP2D6*2 have diminished CYP2D6 metabolic capacity for perhexiline.
An efficient vector-space approach for accurately modeling fixed-point digital signal processors (2002)

This paper presents the DSP Software Engineering (DSPSE) Fixed Arithmetic Tool (FAT/sup TM/) method for modeling DSP fixed-point processors and converting an algorithm from a floating-point model to a given DSP fixed-point processor model. All DSP fixed-point processors have associated with them a set of fixed bit length data representations for the storage and manipulation of binary information. We define a vector sub-space for each DSP fixed-point processor, as a direct sum of each distinct fixed bit length data representation sub-space. The direct sum of all DSP fixed-point processor vector subspaces forms a FAT/sup TM/ DSP vector space. Furthermore, for our discussion, we define an operator projection to be performed on our DSP vector space such that redundancy in the operational behavior of the DSPs to be modeled can be exploited. In our preferred embodiment we implement the FAT/sup TM/ vector space in a C++ environment, resulting in a Fixed Arithmetic C++ Tool (FACT/sup TM/) library for all associated fixed-point DSPs. For our own development we have created, a FACT/sup TM/ library for the Texas Instrument TMS320C54x DSP fixed-point processor. The TMS320C54x library has been used in the development of the Japanese Vector Sum Excited Linear Prediction (JVSELP) algorithm and the International Telecommunications Union G.728 standard algorithm.
Floor plan generation and room labeling of indoor environments from laser range data (2015)

Automatic generation of building floor plans is useful in many emerging applications, including indoor navigation, augmented and virtual reality, as well as building energy simulation software. These applications require watertight models with limited complexity. In this paper, we present an approach that produces 2.5D extruded watertight models of building interiors from either 2D particle filter grid maps or full 3D point-clouds captured by mobile mapping systems. Our approach is to triangulate a 2D sampling of wall positions and separate these triangles into interior and exterior sets. We partition the interior volume of the building model by rooms, then simplify the model to reduce noise. Such labels are useful for building energy simulations involving thermal models, as well as for ensuring geometric accuracy of the resulting 3D model. We experimentally verify the performance of our proposed approach on a wide variety of buildings. Our approach is efficient enough to be used in real-time in conjunction with Simultaneous Localization and Mapping (SLAM) applications. 1
Rapidly Cross-Linkable DOPA Containing Terpolymer Adhesives and PEG-Based Cross-Linkers for Biomedical Applications (2012)

A new three-component bio-inspired adhesive was synthesized that is a terpolymer composed of a water-soluble segment, an interfacial adhesion segment, and a cross-linking segment. Strong wet adhesion properties are obtained utilizing a 3,4-dihydroxy-l-phenylalanine (DOPA) moiety. Poly(acrylic acid) provides high water solubility due to strong ionic interactions with water. An acrylic acid N-hydroxysuccinimide ester (NHS) was included in the adhesive polymer to allow rapid cross-linking with thiol-terminated, 3-armed poly(ethylene glycol) cross-linking agents. The thiol terminal poly(ethylene glycol) was designed to be bulky to avoid possible penetration of molecules to the cell and tissue. The NHS and thiol groups react within 30 s to form covalent bonds. This design allows for rapid optimization of properties for specific applications. Lap shear strength tests on wet porcine skin demonstrated a 190% increased value in adhesion strength for adhesives having the DOPA moiety. After cross-linking, adhesion was enhanced by 450% over poly(acrylic acid-co-acrylic acid NHS) and was 240% higher than un-cross-linked poly(acrylic acid-co-acrylic acid NHS-co-N-methacryloyl-3,4-dihydroxyl-l-phenylalanine). Rheology studies show adhesive viscosity drops significantly at high shear rates, demonstrating its potential to be injected via syringe. The cross-linked adhesive displayed much stronger mechanical properties and higher elastic and viscous moduli than an un-cross-linked adhesive model. Furthermore, the cross-linked adhesive has enhanced moduli near body temperature (38 °C) as compared to room temperature (23 °C), increasing the applications as a biomedical adhesive.
Leukocyte Adhesion Deficiency (1989)

Leukocyte adhesion deficiency (LAD) is a heritable deficiency of the LFA-1, Mac-1, p150,95 family of leukocyte alpha beta heterodimers (the leukocyte integrins). We have studied the defect in patients who synthesize an aberrantly small form of the beta subunit common to all three proteins. S1 nuclease protection showed the presence of a 90-nucleotide mismatch in RNA from patients and relatives, correlating with inheritance of the disease. Use of the Taq polymerase chain reaction to amplify this region of RNA after first strand cDNA synthesis and sequencing showed an in-frame deletion of 90 nucleotides in the extracellular domain. Thus, this highly conserved region, 63% and 53% identical in amino acid sequence to two other beta subunits of the integrin family, is required for association of the beta subunit with alpha subunits. The 90-nucleotide region corresponds to a single exon present in both the normal and patient genome. The patient DNA has a single G to C substitution in the 5' splice site. This results in the direct joining of nonconsecutive exons in an unusual type of abnormal RNA splicing. A small amount of normally spliced message, detected by S1 nuclease protection and Taq polymerase chain reaction, encodes a normal sized beta subunit which is surface-expressed and accounts for the low levels of leukocyte integrin expression observed in these patients, and hence the moderate phenotype.
High-density lipoprotein cholesterol and coronary heart disease in hypercholesterolemic men: the Lipid Research Clinics Coronary Primary Prevention Trial. (1986)

Plasma levels of high-density lipoprotein cholesterol (HDL-C) at entry and subsequent changes from these baseline levels were inversely predictive of coronary heart disease (CHD) end points in hypercholesterolemic men followed for 7 to 10 years in the Lipid Research Clinics Coronary Primary Prevention Trial, especially in the 1907 participants receiving cholestyramine. When the men in this cohort were compared, each 1 mg/dl increment in baseline HDL-C (mean 44.3 mg/dl) was associated with a 5.5% decrement in risk of "definite" CHD death or myocardial infarction (Z = -5.4), and each 1 mg/dl increase from baseline HDL-C levels (mean increase = 1.6 mg/dl) during the trial was associated with a 4.4% risk reduction (Z = -2.2). In the 1899 participants receiving placebo, the corresponding risk decrements were 3.4% and 1.1%. Although baseline HDL-C level (mean = 44.4 mg/dl) remained a significant risk predictor (Z = -3.8) in the placebo cohort, increases in HDL-C (mean increase 0.5 mg/dl) were not significantly predictive of CHD (Z = -0.6) unless "suspect" as well as "definite" end points were analyzed (Z = -2.0). When the associations between HDL-C (baseline plus change) and incidence of definite CHD end points within each treatment cohort were compared, their difference approached nominal significance (Z = 1.9). The results suggest a synergistic interaction, in which cholestyramine treatment reduced CHD risk most substantially in men maintaining the highest HDL-C levels.
Battered Women & Feminist Lawmaking: Author Meets Readers (2002)

ELIZABETH M. SCHNEIDER** is the Rose L. Hoffer Professor of Law and Chair of the Edward V. Sparer Public Interest Law Fellowship Program at Brooklyn Law School. She is the author of Battered Women and Feminist Lawmaking (Yale University Press 2000), which won the 2000 Professional/ Scholarly Publishing Award of the Association of American Publishers, Legal Category. She is the coauthor of the law school
A digitally stabilized type-III PLL using ring VCO with 1.01ps&lt;inf&gt;rms&lt;/inf&gt; integrated jitter in 65nm CMOS (2012)

The design of low-jitter VCO-based PLLs is quite challenging as high VCO control gain, K <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">VCO</sub> , increases the phase noise contribution arising from the charge pump and loop filter. To resolve this problem, dual-tuning PLLs (DT-PLLs) have been studied [1-4]. The DT-PLL structure adds a narrow-bandwidth coarse (high-K <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">VCO</sub> ) path to the fine (low-K <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">VCO</sub> ) path consisting of a type-II PLL. The narrow-bandwidth analog filter in the coarse path plays an important role in preventing the charge pump and the loop filter from increasing the output jitter, while a wide-tuning range is maintained. Moreover, the coarse path adds another pole at origin to the fine path and transforms it from a type-II to type-III PLL [3-4]. Compared to a type-II PLL, owing to its boosted low-frequency loop gain, a type-III PLL can better suppress a low-frequency disturbance to the ring VCO, such as temperature drift. However, a type-III PLL has stability problems. To ensure sufficient phase margin (PM), a type-III PLL requires an extremely narrow-bandwidth (e.g. ~10-100Hz) analog filter in the coarse path or must make the K <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">VCO</sub> of the fine path larger. The former requires a nano-Farad capacitor or a fairly complex design for shrinking the capacitance, while the latter way increases total jitter. This paper presents a digitally stabilized type-Ill PLL with a ring VCO. It employs a DT-PLL structure and improves its stability by composing the coarse path with a digital integrator and a digital-to-analog converter (DAC). It can set the K <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">vco</sub> of the fine path to 10MHz/V, which is 1000χ lower than that of the coarse path with a sufficient PM. For further in-band phase-noise reduction, the proposed type-Ill PLL adopts a sub-sampling PLL (SS-PLL) in its fine path [5], and achieves 1.01 ps <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rms</sub> integrated jitter.
(Zr,Hf)Co(Sb,Sn) half-Heusler phases as high-temperature (&amp;gt;700°C) p-type thermoelectric materials (2008)

By substituting Sn for Sb, the potential of stable (Zr,Hf)Co(Sb,Sn) half-Heusler phases, as p-type thermoelectric materials, for high-temperature power generation has been examined. Sn concentration as much as ∼20%–30% is required to realize high power factor values. Substitution of heavier Hf, which reduces the thermal conductivity (κ) via mass fluctuation scattering, nonetheless maintains high mobility. As a result, the thermoelectric figure of merit ZT, for these not-yet-optimized materials, which we found to be ZT=0.5 at 1000K (measured) and ZT=0.6 at 1100K (extrapolated), surpasses the industry benchmark for a p-type material set by SiGe alloys.
Characterization of two post-translationally processed forms of human serum retinol-binding protein: altered ratios in chronic renal failure. (1995)

Retinol-binding protein (RBP) is the specific blood carrier for the transport of retinol (vitamin A) to target tissues. As the kidney is involved in RBP metabolism, the analysis of RBP species in the serum of patients with chronic renal failure (CRF) was used as a model to study possible RBP alterations. SDS-PAGE-immunoblotting analysis of normal and CRF sera shows a doublet of RBP bands (band A and band B) near 21 kDa. Mass spectrometric analysis of purified RBPs from CRF and normal sera revealed the presence not only of full-length RBP (183 residues, migrating in band A) but also two forms of RBP differing from the native form by the loss of C-terminal Leu (i.e., RBP1 (residues 1-182), migrating in band A also) and the loss of C-terminal Leu-Leu (i.e., RBP2 (residues 1-181), migrating in band B). Interestingly, RBP2 was considerably increased in the serum of CRF, whereas it was low in normal sera. In healthy retinol target-tissues and in cultured HepG2 cells, RBP2 levels were significantly and variably present compared to RBP and RBP1. We propose that these post-translationally modified forms of RBP occur in cells and that after their release into the blood circulation RBP2 is cleared by the kidney in healthy individuals but accumulates in the serum of CRF patients. RBP2 may have an important physiological role in retinol transport and/or recycling.
Power System Analysis Software Package (PSASP)-an integrated power system analysis tool (2002)

In this paper, an integrated power system analysis tool called Power System Analysis Software Package (PSASP) is presented. A brief description of every constituent module of PSASP is given first, then user-defined (UD) modeling function and user program interface (UPI) are introduced further. Finally, the new development of PSASP, power system analysis software platform, is illustrated through a diagram.
Characteristics of high performance YBa2Cu3O7 step-edge junctions (1992)

Step-edge Josephson junctions are engineered grain boundary junctions fabricated using standard lithographic and film deposition techniques. We report a systematic study of 180 YBa2Cu3O7 step-edge junctions and identify a fabrication technique which results in a 90% yield of working junctions with critical current spreads from 30% to 50% (1σ/Ic-ave)over the entire substrate. Technically useful critical current values at 65 K can be obtained by adjusting YBa2Cu3O7 film thickness. IcRn values, approximately independent of film thickness, are ∼1 mV at 4.2 K and ∼0.1 mV at 65 K. Most junctions exhibit ideal electrical behavior in accordance with the RSJ model.
Computationally Sound Proofs (2000)

This paper puts forward a new notion of a proof based on computational complexity and explores its implications for computation at large. Computationally sound proofs provide, in a novel and meaningful framework, answers to old and new questions in complexity theory. In particular, given a random oracle or a new complexity assumption, they enable us to prove that verifying is easier than deciding for all theorems; provide a quite effective way to prove membership in computationally hard languages (such as ${\cal C}o$-$\cal N \cal P$-complete ones); and show that every computation possesses a short certificate vouching its correctness. Finally, if a special type of computationally sound proof exists, we show that Blum's notion of program checking can be meaningfully broadened so as to prove that $\cal N \cal P$-complete languages are checkable.