gebhart/patrepeval huggingface dataset:
Biochemical and Functional Characterization of Orai Proteins (2007)

Stimulation of immune cells triggers Ca2+ entry through store-operated Ca2+ release-activated Ca2+ channels, promoting nuclear translocation of the transcription factor NFAT. Through genome-wide RNA interference screens in Drosophila, we and others identified olf186-F (Drosophila Orai, dOrai) and dStim as critical components of store-operated Ca2+ entry and showed that dOrai and its human homologue Orai1 are pore subunits of the Ca2+ release-activated Ca2+ channel. Here we report that Orai1 is predominantly responsible for store-operated Ca2+ influx in human embryonic kidney 293 cells and human T cells and fibroblasts, although its paralogue Orai3 can partly compensate in the absence of functional Orai1. All three mammalian Orai are widely expressed at the mRNA level, and all three are incorporated into the plasma membrane. In human embryonic kidney 293 cells, Orai1 is glycosylated at an asparagine residue in the predicted second extracellular loop, but mutation of the residue does not compromise function. STIM1 and Orai1 colocalize after store depletion, but Orai1 does not associate detectably with STIM1 in glycerol gradient centrifugation or coimmunoprecipitation experiments. Glutamine substitutions in two conserved glutamate residues, located within predicted transmembrane helices of Drosophila Orai and human Orai1, greatly diminish store-operated Ca2+ influx, and primary T cells ectopically expressing mutant E106Q and E190Q Orai1 proteins show reduced proliferation and cytokine secretion. Together, these data establish Orai1 as a predominant mediator of store-operated calcium entry, proliferation, and cytokine production in T cells.
Preparation of YBa2Cu3O7−x and Bi2Sr2CaCu2Oy Films by Electrodeposition Technique (1991)

Abstract The high-temperature superconducting YBa2Cu3O7−x and Bi2Sr2CaCu2Oy phases have been prepared by the electrodeposition of the corresponding precursors from the baths containing salts of the constituent metals in dimethylsulfoxide–H2O mixture solvent followed by a suitable heat treatment. The use of this solvent was found to contribute to a significant improvement in the deposition process.
A promoter-level mammalian expression atlas (2014)

Regulated transcription controls the diversity, developmental pathways and spatial organization of the hundreds of cell types that make up a mammal. Using single-molecule cDNA sequencing, we mapped transcription start sites (TSSs) and their usage in human and mouse primary cells, cell lines and tissues to produce a comprehensive overview of mammalian gene expression across the human body. We find that few genes are truly 'housekeeping', whereas many mammalian promoters are composite entities composed of several closely separated TSSs, with independent cell-type-specific expression profiles. TSSs specific to different cell types evolve at different rates, whereas promoters of broadly expressed genes are the most conserved. Promoter-based expression analysis reveals key transcription factors defining cell states and links them to binding-site motifs. The functions of identified novel transcripts can be predicted by coexpression and sample ontology enrichment analyses. The functional annotation of the mammalian genome 5 (FANTOM5) project provides comprehensive expression profiles and functional annotation of mammalian cell-type-specific transcriptomes with wide applications in biomedical research.
Identification of N-Linked Glycoproteins in Human Saliva by Glycoprotein Capture and Mass Spectrometry (2006)

Glycoproteins make up a major and important part of the salivary proteome and play a vital role in maintaining the health of the oral cavity. Because changes in the physiological state of a person are reflected as changes in the glycoproteome composition, mapping the salivary glycoproteome will provide insights into various processes in the body. Salivary glycoproteins were identified by the hydrazide coupling and release method. In this approach, glycoproteins were coupled onto a hydrazide resin, the proteins were then digested and formerly N-glycosylated peptides were selectively released with the enzyme PNGase F and analyzed by LC-MS/MS. Employing this method, coupled with in-solution isoelectric focusing separation as an additional means for pre-fractionation, we identified 84 formerly N-glycosylated peptides from 45 unique N-glycoproteins. Of these, 16 glycoproteins have not been reported previously in saliva. In addition, we identified 44 new sites of N-linked glycosylation on the proteins.
Effect of Baseline or Changes in Adrenergic Activity on Clinical Outcomes in the β-Blocker Evaluation of Survival Trial (2004)

In BEST, a subset of patients treated with bucindolol had an increased risk of death as the result of sympatholysis, which compromised the efficacy of this third-generation beta-blocker.
Quiver: Consistent and Scalable Object Sharing for Edge Services (2008)

We present Quiver, a system that coordinates service proxies placed at the "edge" of the Internet to serve distributed clients accessing a service involving mutable objects. Quiver enables these proxies to perform consistent accesses to shared objects by migrating the objects to proxies performing operations on those objects. These migrations dramatically improve performance when operations involving an object exhibit geographic locality, since migrating this object into the vicinity of proxies hosting these operations will benefit all such operations. Other workloads benefit from Quiver, dispersing the computation load across the proxies and saving the costs of sending operation parameters over the wide area when these are large. Quiver also supports optimizations for single-object reads that do not involve migrating the object. We detail the protocols for implementing object operations and for accommodating the addition, involuntary disconnection, and voluntary departure of proxies. We also evaluate Quiver through experiments on PlanetLab. Finally, we discuss the use of Quiver to build an e-commerce application and a distributed network traffic modeling service.
Preparation and Characterization of Crosslinked Porous Silk Fibroin Gel. (1998)

In order to develop new biomedical materials, the preparation and characterization of crosslinked porous silk fibroin gel (CPFG) were investigated by using ethylene glycol diglycidyl ether (EGDE) as a crosslinking agent. The CPFG was prepared by freezing (-50°C_??_-5°C) and thawing a homogeneous fibroin solution containing EGDE. The average pore size of CPFG was varied from 100-300μm by adjusting the concentrations of silk fibroin, EGDE and the freezing temperature. The tenacity and elongation ratio of CPFG were improved by increasing the concentration of silk fibroin and EGDE within a certain range. From these results, it was found that CPFG with excellent properties can be prepared at low temperatures by using EGDE as a crosslinking agent.
Synthesis and chemistry of trifluoroacetyl hypofluorite with elemental fluorine. A novel method for synthesis of .alpha.-fluorohydrins (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and chemistry of trifluoroacetyl hypofluorite with elemental fluorine. A novel method for synthesis of .alpha.-fluorohydrinsShlomo Rozen and Ori LermanCite this: J. Org. Chem. 1980, 45, 4, 672–678Publication Date (Print):February 1, 1980Publication History Published online1 May 2002Published inissue 1 February 1980https://pubs.acs.org/doi/10.1021/jo01292a025https://doi.org/10.1021/jo01292a025research-articleACS PublicationsRequest reuse permissionsArticle Views475Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
High and selective expression of yeast cytosine deaminase under a carcinoembryonic antigen promoter-enhancer. (2002)

Yeast cytosine deaminase (yCD)-based gene therapy offers the potential for selective production of the cytotoxic and radiosensitizing drug 5-fluorouracil (5-FU) from the benign prodrug 5-fluorocytosine within colorectal cancers. Although previous attempts to target therapy to colorectal cancer using the carcinoembryonic antigen (CEA) promoter have demonstrated specificity, this has been achieved at the cost of 10- to 300-fold loss in activity compared with strong but nonspecific rous sarcoma virus (RSV) or cytomegalovirus promoters. We developed a highly specific and active gene transfer method for colorectal cancer using CEA under control of a promoter-enhancer. We compared the RSV promoter-derived with the CEA promoter-enhancer-derived transgene expression in 10 different cell lines with differing CEA status. We found that the transgene expression resulting from both transient transfection and adenoviral infection with the CEA promoter-enhancer was as strong as the RSV promoter while maintaining specificity for CEA-producing cell lines. For instance, when we compared yCD expression between LoVo (CEA+) and human fibroblast (CEA-), we found a 30-fold-increased yCD expression in LoVo cells from CEA-enhancer adenovirus although there was no difference in the yCD expression between the cell lines when infected with RSV/yCD virus. This specificity was also achieved while maintaining a higher yCD enzyme activity than we obtained with RSV/yCD adenovirus in an HT-29 intrahepatic tumor model. We then compared the response of HT-29 xenografts to treatment with 5-fluorocytosine and yCD adenovirus driven by either the RSV or the CEA promoter-enhancer and found similar tumor growth inhibition. These findings suggest that the CEA promoter-enhancer strategy confers specificity while preserving activity and is worth exploring in additional animal and, potentially, clinical trials.
Audio Subband Coding With Improved Representation Of Transient Signal Segments (1998)

Publication in the conference proceedings of EUSIPCO, Rhodes, Greece, 1998
A Novel Inactivated Intranasal Respiratory Syncytial Virus Vaccine Promotes Viral Clearance without Th2 Associated Vaccine-Enhanced Disease (2011)

These studies indicate that nanoemulsion-based inactivated RSV vaccination can augment viral-specific immunity, decrease mucus production and increase viral clearance, without evidence of Th2 immune mediated pathology.
Bispecific T-Cell Engaging Antibodies for Cancer Therapy (2009)

There is increasing evidence that T cells are able to control tumor growth and survival in cancer patients, both in early and late stages of the disease. However, tumor-specific T-cell responses are difficult to mount and sustain in cancer patients, and are limited by numerous immune escape mechanisms of tumor cells selected during immunoediting. An alternative approach to engage T cells for cancer therapy are antibodies, which are bispecific for a surface target antigen on cancer cells, and for CD3 on T cells. These are capable of connecting any kind of cytotoxic T cell to a cancer cell, independently of T-cell receptor specificity, costimulation, or peptide antigen presentation. Here, we review the principle of a new class of bispecific antibodies called BiTE (for "bispecific T-cell engager") antibodies. Recent results from clinical studies with a CD19/CD3-bispecific BiTE antibody suggest that this therapeutic paradigm is finally showing promise for treatment of both bulky and minimal residual disease.
Water Treatment Unit Processes: Physical and Chemical (2006)

The unit process approach, common in the field of chemical engineering, was introduced about 1962 to the field of environmental engineering. An understanding of unit processes is the foundation for continued learning and for designing treatment systems. The time is ripe for a new textbook that delineates the role of unit process principles in environmental engineering. Suitable for a two-semester course, Water Treatment Unit Processes: Physical and Chemical provides the grounding in the underlying principles of each unit process that students need in order to link theory to practice. Bridging the gap between scientific principles and engineering practice, the book covers approaches that are common to all unit processes as well as principles that characterize each unit process. Integrating theory into algorithms for practice, Professor Hendricks emphasizes the fundamentals, using simple explanations and avoiding models that are too complex mathematically, allowing students to assimilate principles without getting sidelined by excess calculations. Applications of unit processes principles are illustrated by example problems in each chapter. Student problems are provided at the end of each chapter; the solutions manual can be downloaded from the CRC Press Web site. Excel spreadsheets are integrated into the text as tables designated by a CD prefix. Certain spreadsheets illustrate the idea of scenarios that emphasize the idea that design solutions depend upon assumptions and the interactions between design variables. The spreadsheets can be downloaded from the CRC web site.The book has been designed so that each unit process topic is self-contained, with sidebars and examples throughout the text. Each chapter has subheadings, so that students can scan the pages and identify important topics with little effort. Problems, references, and a glossary are found at the end of each chapter. Most chapters contain downloadable Excel spreadsheets integrated into the text and appendices with additional information. Appendices at the end of the book provide useful reference material on various topics that support the text. This design allows students at different levels to easily navigate through the book and professors to assign pertinent sections in the order they prefer. The book gives your students an understanding of the broader aspects of one of the core areas of the environmental engineering curriculum and knowledge important for the design of treatment systems.
Ketenes. X. Heterocyclic Systems Derived from Dimethylmalonyl Chloride<sup>1</sup> (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKetenes. X. Heterocyclic Systems Derived from Dimethylmalonyl Chloride1James C. Martin, Kent C. Brannock, and Ronald H. MeenCite this: J. Org. Chem. 1966, 31, 9, 2966–2972Publication Date (Print):September 1, 1966Publication History Published online1 May 2002Published inissue 1 September 1966https://pubs.acs.org/doi/10.1021/jo01347a051https://doi.org/10.1021/jo01347a051research-articleACS PublicationsRequest reuse permissionsArticle Views114Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Parallelized network security protocols (2002)

Security and privacy are growing concerns in the Internet community, due to the Internet's rapid growth and the desire to conduct business over it safely. This desire has led to the advent of several proposals for security standards, such as secure IP, secure HTTP, and the Secure Socket Layer. All of these standards propose using cryptographic protocols such as DES and RSA. Thus, the need to use encryption protocols is increasing. Shared-memory multiprocessors make attractive server platforms, for example as secure World-Wide Web servers. These machines are becoming more common, as shown by recent vendor introductions of platforms such as SGI's Challenge, Sun's SPARCCenter, and DEC's AlphaServer. The spread of these machines is due both to their relative ease of programming and their good price/performance. This paper is an experimental performance study that examines how encryption protocol performance can be improved by using parallelism. We show linear speedup for several different Internet-based cryptographic protocol stack running on a symmetric shared-memory multiprocessor using two different approaches to parallelism.
Coumarin Derivatives for Medicinal Purpose. V (1953)

Sedative and hypnotic actions, and toxicity against mice were tested with 20 kinds of coumarin derivatives synthesized and three kinds of market products. Efficacy and toxicity of each preparation were compared by the values of lethal dose, LD60, hypnotic dose, HD60, and sedative dose, SD60, calculated by the Van der Waerden's Flächenmethode and by the Behrens-Kärber method (cf. Tables and Figs.). Results obtained can be summarized as follows:1) Efficacy is lost when -CH2- is introduced between the coumarin ring and CO-N(C2H5)2 of coumarin-3-carboxylic acid diethylamide to coumarin-3-acetic acid diethylamide.2) Exchange of -CO- in coumarin-3-carboxylic acid diethylamide with -O- to isocou-marincarboxylic acid diethylamide did not affect the efficacy to any great extent, but its methyl ester lacked any efficacy.3) Introduction of -COOH, -COOCH3, or -COOCH2H5, in the 5-position of α-pyrone ring, in place of the benzene ring, in coumarin nucleus, did not add any efficacy.4) In any case, free carboxylic acid possessed no efficacy.5) Coumarin itself did not possess any efficacy but 2-thiocoumarin was effective.6) Hydrogenation of the double bond between 3- and 4-positions decreased the efficacy, with the exception of coumarin-3-acetic acid diethylamide.7) Efficacy was not shown by the introduction of -NO2 and -NH2 in the 6-position, of isoamyl in the 6-position, and -OH in the 7-position or of -COCH3 in the 3-position of the coumarin ring.8) The sodium salt of barbital used as the control was more weaker in both efficacy and toxicity than the free base.
Identification of Malignant Cells In Serous Effusions Using A Panel of Monoclonal Antibodies Ber‐Ep4, Mca‐B‐12 and Ema (1992)

A panel of three monoclonal antibodies (MoAbs) was tested on 29 benign and 53 malignant effusions with the aim of investigating its usefulness for the discrimination between benign and malignant lesions. The panel consisted of MoAbs directed against epithelial membrane antigen (EMA); MCA-b-12, reacting with a 350 kD glycoprotein with mucin-like characteristics present on human breast cancer cells and various other normal and neoplastic tissues, and Ber-EP4, directed against a 34 and 39 kD glycopeptide on human epithelial cells but not on mesothelium. Fifty-two (98%) of the malignant effusions reacted with EMA, 49 (92%) with MCA-b-12 and 44 (83%) with Ber-EP4. Fourteen per cent of benign effusions reacted with EMA, 17% with MCA-b-12 and 7% with Ber-EP4. All seven effusions obtained from patients with a malignant mesothelioma reacted with EMA, six of the seven cases staining intensively. None of the seven stained with Ber-EP4. MCA-b-12 did not react with the cells in one case of malignant mesothelioma. The results suggest that the combination of EMA and Ber-EP4 may be used to discriminate between benign and malignant cells and possibly also between adenocarcinoma and malignant mesothelioma. MCA-b-12 followed in general the reaction pattern of EMA, although often with a less intense staining reaction, making this antibody unsuitable for inclusion in the panel.
An Original Low-Loss and Pass-Band Flattened Si0/sub 2/ on Si Planar Wavelength Demultiplexer (2005)

Flattening of the passband response of wavelength demultiplexers is desirable for multiwavelength transmission because it relaxes the tolerance on the transmitted wavelengths.
Optimal Pivot Selection Method Based on the Partition and the Pruning Effect for Metric Space Indexes (2011)

This paper proposes a new method to reduce the cost of nearest neighbor searches in metric spaces. Many similarity search indexes recursively divide a region into subregions by using pivots, and construct a tree-structured index. Most of recently developed indexes focus on pruning objects and do not pay much attention to the tree balancing. As a result, indexes having imbalanced tree-structure may be constructed and the search cost is degraded. We propose a similarity search index called the Partitioning Capacity (PC) Tree. It selects the optimal pivot in terms of the PC that quantifies the balance of the regions partitioned by a pivot as well as the estimated effectiveness of the search pruning by the pivot. As a result, PCTree reduces the search cost for various data distributions. We experimentally compared PCTree with four indexes using synthetic data and five real datasets. The experimental results shows that the PCTree successfully reduces the search cost.
Anderson localization of one-dimensional wave propagation on a fluid-loaded plate (1992)

The propagation of flexural waves along a fluid-loaded plate with an irregular array of line attachments is examined. The system is shown to be equivalent to a one-dimensional chain with a nearest neighbor coupling arising from the fluid-loaded wave, and a long-range coupling due to bulk waves in the fluid. The magnitude of the long-range coupling is shown to be small compared to the nearest neighbor coupling for many applications. The irregularity will thus produce Anderson localization, at least for moderate distances. The equations of motion are numerically solved for two cases: (1) a sparse array for which the long-range coupling is expected to be negligible, and (2) a dense array for which the long-range coupling is fairly small, but not negligible. Unambiguous Anderson localization effects were observed in both cases.