gebhart/patrepeval huggingface dataset:
Exosome release of ADAM15 and the functional implications of human macrophage‐derived ADAM15 exosomes (2012)

A disintegrin and metalloproteinase 15 (ADAM15), the only ADAM protein containing an Arg-Gly-Asp (RGD) motif in its disintegrin-like domain, is a widely expressed membrane protein that is involved in tumor progression and suppression. However, the underlying mechanism of ADAM15-mediated tumor suppression is not clearly understood. This study demonstrates that ADAM15 is released as an exosomal component, and ADAM15 exosomes exert tumor suppressive activities. We found that exosomal ADAM15 release is stimulated by phorbol 12-myristate 13-acetate, a typical protein kinase C activator, in various tumor cell types, and this results in a corresponding decrease in plasma membrane-associated ADAM15. Exosomes rich in ADAM15 display enhanced binding affinity for integrin αvβ3 in an RGD-dependent manner and suppress vitronectin- and fibronectin-induced cell adhesion, growth, and migration, as well as in vivo tumor growth. Exosomal ADAM15 is released from human macrophages, and macrophage-derived ADAM15 exosomes have tumor inhibitory effects. This work suggests a primary role of ADAM15 for exosome-mediated tumor suppression, as well as functional significance of exosomal ADAM protein in antitumor immunity.
Chromosome analysis by high illumination flow cytometry (1983)

Fluorescence measurements from metaphase chromosomes of the Chinese hamster, stained with propidium iodide excited at high illumination irradiance, completely resolve each chromosome type. The measurements are performed in a specially designed flow cytometer that achieves high irradiance (4 MW/cm2) by using high power laser output (2 W at 488 nm) focused to small spot size (1% irradiance variation over 2 microns). The coefficient of variation of each chromosome peak is near 1.5%. Saturation of the fluorescence transition and photobleaching, two consequences of high irradiance, are shown to occur. Even with a nonlinear dependence of fluorescence upon illumination irradiance, fluorescence retains a proportional response to chromosome type; each chromosome peak maintains a consistent ratio to the others at every irradiance. No perturbation of fluorescence by the optical or geometrical properties of the chromosomes is evident. The advantages of high irradiance illumination are an increase in fluorescence sufficient to reduce the statistical error in photoelectron number to a low level and reduced influence of laser power fluctuations and variable chromosome flow trajectories on the precision. These benefits improve the resolution of chromosome analysis by flow cytometry, particularly the resolution of smaller chromosomes.
Evolution of SIM provisioning towards a flexible MCIM provisioning in M2M vertical industries (2012)

The fast growing market for wireless Machine-to-Machine (M2M) communication services is facing specific challenges which do not exist comparably in the traditional cellular subscriber market. The challenges are emerging around the changed ecosystem and value chain and dealing with different traffic and load profiles in the network. The significantly reduced ARPDs (average revenue per device) are calling for increased operational efficiency and flexibility and tangible cost reductions. With regard to the requirements arising in this context this paper focuses on new concepts for SIM management (SIM: Subscriber Identity Module) in the M2M domain from a subscription management perspective in order to develop an economic MCIM provisioning process (MCIM: M2M communication identity module). Three technical concepts were addressed and described with a look on the critical features. Essential items are the circle of trust, the grade of flexibility, the complexity of the solution and the impact on security. A further evolution of the Embedded UICC (Universal Integrated Circuit Card) proposal currently handled in standardization is presented and discussed, which gives the device owner and the M2M service provider a more active role and responsibility in the process. This approach enables full flexibility for change of operator and subscription and requires less administrative effort from MNOs (Mobile Network Operators), in particular for devices currently not requiring an active subscription. The respective business processes for MCIM management are presented as blueprints and discussed with regard to the lifecycle phases. There is a need for an increased grade of automation and integration of these workflows going beyond the boundaries of a particular stakeholder participating in the value net.
Gene expression of CC chemokines in experimental crescentic glomerulonephritis (CGN) (1997)

CGN is a rapidly progressive glomerular disease. Monocytes/macrophages are frequently observed in glomeruli in cases of CGN and they are considered to play a crucial role in the pathogenesis of this disease. We described previously the glomerular expression of monocyte chemoattractant protein-1 (MCP-1), which is a potent chemoattractant for monocytes and a member of CC chemokine family, in an experimental model of CGN. In the present study we investigated the expression of mRNAs for other CC chemokines, namely, MCP-3, macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, RANTES and TCA3, all of which are chemotactic for monocytes, in the CGN model. First, we established a reverse transcriptase-polymerase chain reaction (RT-PCR) method by which mRNA for each of the CC chemokines could be amplified separately, and then we measured the levels of the expression of mRNAs for the chemokines in diseased glomeruli at several time points after induction of CGN. The mRNAs for all CC chemokines examined were expressed in glomeruli of rats with CGN. Moreover, induction of the gene expression of MIP-1alpha and MIP-1beta seemed to occur earlier than that of the others. CC chemokines may contribute to the recruitment and activation of monocytes in CGN, and each individual CC chemokine may play an overlapping but distinct role in the pathogenesis of this disease.
New Attempt at the Synthesis of Lavandulol by a Claisen Type Rearrangement (1968)

Journal Article New Attempt at the Synthesis of Lavandulol by a Claisen Type Rearrangement Get access Masanao Matsui, Masanao Matsui Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo Search for other works by this author on: Oxford Academic Google Scholar Bernard Stalla-Bourdillon Bernard Stalla-Bourdillon Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo Pensionnaire scientifique à la Maison franco-japonaise de Tokyo, 3-Nichome, Surugadai, Kanda, Chiyoda-ku, Tokyo Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 32, Issue 10, 1 October 1968, Pages 1246–1249, https://doi.org/10.1080/00021369.1968.10859212 Published: 01 October 1968 Article history Received: 30 April 1968 Published: 01 October 1968
A New Theory of Phylogeny Inference Through Construction of Multidimensional Vector Space (2001)

Here, a new theory of molecular phylogeny is developed in a multidimensional vector space (MVS). The molecular evolution is represented as a successive splitting of branch vectors in the MVS. The end points of these vectors are the extant species and indicate the specific directions reflected by their individual histories of evolution in the past. This representation makes it possible to infer the phylogeny (evolutionary histories) from the spatial positions of the end points. Search vectors are introduced to draw out the groups of species distributed around them. These groups are classified according to the nearby order of branches with them. A law of physics is applied to determine the species positions in the MVS. The species are regarded as the particles moving in time according to the equation of motion, finally falling into the lowest-energy state in spite of their randomly distributed initial condition. This falling into the ground state results in the construction of an MVS in which the relative distances between two particles are equal to the substitution distances. The species positions are obtained prior to the phylogeny inference. Therefore, as the number of species increases, the species vectors can be more specific in an MVS of a larger size, such that the vector analysis gives a more stable and reliable topology. The efficacy of the present method was examined by using computer simulations of molecular evolution in which all the branch- and end-point sequences of the trees are known in advance. In the phylogeny inference from the end points with 100 multiple data sets, the present method consistently reconstructed the correct topologies, in contrast to standard methods. In applications to 185 vertebrates in the alpha-hemoglobin, the vector analysis drew out the two lineage groups of birds and mammals. A core member of the mammalian radiation appeared at the base of the mammalian lineage. Squamates were isolated from the bird lineage to compose the outgroup, while the other living reptilians were directly coupled with birds without forming any sister groups. This result is in contrast to the morphological phylogeny and is also different from those of recent molecular analyses.
Adaptive array processing MLSE receivers for TDMA digital cellular/PCS communications (1998)

Array processing is a promising approach for improving quality, coverage, and capacity in digital cellular communication systems. By combining array processing with maximum likelihood sequence estimation (MLSE), intersymbol interference (ISI) introduced by multipath propagation can be mitigated as well. Novel symbol-spaced and fractionally spaced adaptive array processing MLSE receivers are developed for both diversity and phased array antenna configurations. The practical issues of synchronization and channel estimation are addressed. A novel approach to automatic frequency error correction (AFC) is proposed and is shown to be critical when cancelling cochannel interference. Performance is evaluated for the reverse link of the IS-136 TDMA-based digital cellular system. Substantial improvements are obtained over conventional antenna configurations for receiver sensitivity (2.5-4 dB) and over traditional antenna combining when cochannel interference is present (0.5-25 dB).
Advanced Glycation End Products Activate Endothelium Through Signal-Transduction Receptor RAGE (2002)

AGEs, through RAGE, may prime proinflammatory mechanisms in endothelial cells, thereby amplifying proinflammatory mechanisms in atherogenesis and chronic inflammatory disorders.
Anisotropic alignment of non-modified BN nanosheets in polysiloxane matrix under nano pulse width electricity (2010)

Boron nitride (BN) nanosheets/polysiloxane composites were fabricated under nano pulse width electric field to enhance the anisotropic alignment of BN nanosheets in a viscous matrix. BN nanosheets have unique physical and thermal properties and exhibit semiconducting behavior with a wide band gap comparing to carbon graphite. However the researches over one dimensional arrangement of BN nanosheets with application of the electric field are limited. The difficulty of modifying the BN surface with electrophilic metal particles or organic functional groups due to its remarkable chemical inertness is one of the reasons. In this study, we attempted to fabricate anisotropic aligned BN nanosheets in a viscous polysiloxane matrix without surface modification of BN nanosheets by using nano pulse width electricity. The electric responses of various nano-dimensional BN nanoparticles were compared by zeta potential. Two kinds of polysiloxane pre-polymers were used to compare the viscosity effects of the polymer matrix. The anisotropic alignment of none-modified BN nanosheets inside polysiloxane can be controlled with variation of the shape and dimension of BN nanosheets, electrodes, DC electric fields, and by application of nano pulse width electricity.
Antigen loading of dendritic cells with apoptotic tumor cell-preparations is superior to that using necrotic cells or tumor lysates. (2007)

Tumor lysates are in common use for DC-based vaccine manufacturing. However, our data indicate an advantage of apoptotic TC-preparations in regard to antigen loading effectiveness as well as the loaded DC's capacity to activate T-cells.
俄罗斯联邦政府部长会议关于对进出口两用商品(劳务)用于约定目的担保义务履行情况实施检查的决议 (1994)

为了提高俄罗斯联邦政府部长会议国家出口检查制度的效率,特作如下决定:1、批准提出的关于进出口两用商品(劳务)用于约定目的担保义务履行情况的检查条例.2、俄罗斯联邦对外经济联络部负责办理和颁发俄国进口证明书,俄罗斯联邦国家海关委员会负责办理和颁发确认交货证明书。3、俄罗斯联邦对外经济联络部经与俄罗斯联邦政府所属的俄罗斯联邦出口检查委员会和俄罗斯联
286. The hydrothermal chemistry of silicates. Part II. Synthetic crystalline sodium aluminosilicates (1952)

R. M. Barrer and E. A. D. White, J. Chem. Soc., 1952, 1561 DOI: 10.1039/JR9520001561
Effect of Plasma [K+] on the Secretion of Aldosterone (1969)

Levels of adrenal arterial [K+] were varied by close intra-arterial infusion of [K+] enriched solutions to the single autotransplanted adrenal gland of the conscious sheep. Secretion rates of aldosterone, corticosterone and cortisol were determined before and during infusion of KC1 solutions. In both Na replete and Na depleted sheep, elevation of adrenal arterial [K+] was associated with increased aldosterone secretion, even with small (0.5 mEq/1 or less) changes in plasma [K+]. Larger elevations of plasma [K+]—up to about 1 mEq/1—produced correspondingly larger elevations in aldosterone secretion; increases beyond 1 mEq/1 produced little further effect. A similar order of responsiveness to similar elevations of plasma [K+] was seen in Na replete and Na depleted sheep. (Endocrinology85: 381, 1969)
Effective Prevention of Microbial Biofilm Formation on Medical Devices by Low-Energy Surface Acoustic Waves (2006)

Low-energy surface acoustic waves generated from electrically activated piezo elements are shown to effectively prevent microbial biofilm formation on indwelling medical devices. The development of biofilms by four different bacteria and Candida species is prevented when such elastic waves with amplitudes in the nanometer range are applied. Acoustic-wave-activated Foley catheters have all their surfaces vibrating with longitudinal and transversal dispersion vectors homogeneously surrounding the catheter surfaces. The acoustic waves at the surface are repulsive to bacteria and interfere with the docking and attachment of planktonic microorganisms to solid surfaces that constitute the initial phases of microbial biofilm development. FimH-mediated adhesion of uropathogenic Escherichia coli to guinea pig erythrocytes was prevented at power densities below thresholds that activate bacterial force sensor mechanisms. Elevated power densities dramatically enhanced red blood cell aggregation. We inserted Foley urinary catheters attached with elastic-wave-generating actuators into the urinary tracts of male rabbits. The treatment with the elastic acoustic waves maintained urine sterility for up to 9 days compared to 2 days in control catheterized animals. Scanning electron microscopy and bioburden analyses revealed diminished biofilm development on these catheters. The ability to prevent biofilm formation on indwelling devices and catheters can benefit the implanted medical device industry.
Improved Outcome in the Treatment of Trigeminal Neuralgia by Percutaneous Stereotactic Rhizotomy with a New, Curved Tip Electrode (1983)

Abstract Percutaneous stereotactic rhizotomy (PSR) as a method of treatment of trigeminal neuralgia has gained popularity in recent years as techniques of electrode placement and lesion production have improved. However, undesirable side effects including major and minor paresthesias, trigeminal motor root weakness, diplopia, and keratitis continue to occur even in cases where the neuralgia is treated successfully. In an attempt to improve treatment further, we have developed an electrode with a flexible curved tip for PSR of the trigeminal nerve. Once the electrode and the cannula are positioned in the retrogasserian portion of the trigeminal nerve with a standardized technique, manipulation of the electrode about its 360° axis readily enables an infinite variety of position adjustments of the electrode tip. This capability enables easier and more precise electrode placement and lesion production. Undesirable lesions of the motor root may be avoided. A curved electrode has been used in 150 patients. The incidence of masseter weakness is 7.3% in this series, compared to 24% in our series of 700 patients treated with a straight electrode. The incidence of undesirable paresthesias has decreased from 27% to 10.6%. The immediate results obtained in patients with the curved electrode have improved. Excellent results (no pain, no side effects) have been achieved in 88% of patients, in contrast to 76% treated with the straight electrode. Application of stereotactic principles and the use of the curved electrode have enhanced the appeal of PSR for the treatment of trigeminal neuralgia.
Synthetic Oligosaccharides as Heparin-Mimetics Displaying Anticoagulant Properties (2003)

Heparin and low molecular weight heparins are major clinical anticoagulants and the drugs of choice for the treatment of deep venous thrombosis. The discovery of an antithrombin binding domain in heparin focused interest on understanding the mechanism of heparin's antithrombotic/ anticoagulant activity. Various heparin-mimetic oligosaccharides have been prepared in an effort to replace polydisperse heparin and low molecular weight heparins with a structurally-defined anticoagulant. The goal of attaining a heparin-mimetic with no unwanted side-effects has also provided motivation for these efforts. This article reviews structure-activity relationship (SAR) of structurally-defined heparin-mimetic oligosaccharides.
IEEE 802.15.7 visible light communication: modulation schemes and dimming support (2012)

Visible light communication refers to shortrange optical wireless communication using visible light spectrum from 380 to 780 nm. Enabled by recent advances in LED technology, IEEE 802.15.7 supports high-data-rate visible light communication up to 96 Mb/s by fast modulation of optical light sources which may be dimmed during their operation. IEEE 802.15.7 provides dimming adaptable mechanisms for flicker-free high-data-rate visible light communication.
C<scp>ysteine</scp> P<scp>eptidases of</scp> M<scp>ammals:</scp> T<scp>heir</scp> B<scp>iological</scp> R<scp>oles and</scp> P<scp>otential</scp> E<scp>ffects in the</scp> O<scp>ral</scp> C<scp>avity and</scp> O<scp>ther</scp> T<scp>issues in</scp> H<scp>ealth and</scp> D<scp>isease</scp> (2002)

Cysteine peptidases (CPs) are phylogenetically ubiquitous enzymes that can be classified into clans of evolutionarily independent proteins based on the structural organization of the active site. In mammals, two of the major clans represented in the genome are: the CA clan, whose members share a structure and evolutionary history with papain; and the CD clan, which includes the legumains and caspases. This review focuses on the properties of these enzymes, with an emphasis on their potential roles in the oral cavity. The human genome encodes at least (but possibly no more than) 11 distinct enzymes, called cathepsins, that are members of the papain family C1A. Ten of these are present in rodents, which also carry additional genes encoding other cathepsins and cathepsin-like proteins. Human cathepsins are best known from the ubiquitously expressed lysosomal cathepsins B, H, and L, and dipeptidyl peptidase I (DPP I), which until recently were considered to mediate primarily "housekeeping" functions in the cell. However, mutations in DPP I have now been shown to underlie Papillon-Lefevre syndrome and pre-pubertal periodontitis. Other cathepsins are involved in tissue-specific functions such as bone remodeling, but relatively little is known about the functions of several recently discovered enzymes. Collectively, CPs participate in multiple host systems that are active in health and in disease. They are involved in tissue remodeling and turnover of the extracellular matrix, immune system function, and modulation and alteration of cell function. Intracellularly, CPs function in diverse processes including normal protein turnover, antigen and proprotein processing, and apoptosis. Extracellularly, they can contribute directly to the degradation of foreign proteins and the extracellular matrix. However, CPs can also participate in proteolytic cascades that amplify the degradative capacity, potentially leading to pathological damage, and facilitating the penetration of tissues by cancer cells. We know relatively little regarding the role of human CPs in the oral cavity in health or disease. Most studies to date have focused on the potential use of the lysosomal enzymes as markers for periodontal disease activity. Human saliva contains high levels of cystatins, which are potent CP inhibitors. Although these proteins are presumed to serve a protective function, their in vivo targets are unknown, and it remains to be discovered whether they serve to control any human CP activity.
Re-annotation and re-analysis of the Campylobacter jejuni NCTC11168 genome sequence (2007)

Major updates were made to genes involved in the biosynthesis of important surface structures such as lipooligosaccharide, capsule and both O- and N-linked glycosylation. This re-annotation will be a key resource for Campylobacter research and will also provide a prototype for the re-annotation and re-interpretation of other bacterial genomes.
Apoptosis is induced by beta-amyloid in cultured central nervous system neurons. (1993)

The molecular mechanism responsible for the neurodegeneration in Alzheimer disease is not known; however, accumulating evidence suggests that beta-amyloid peptide (A beta P) contributes to this degeneration. We now report that synthetic A beta Ps trigger the degeneration of cultured neurons through activation of an apoptotic pathway. Neurons treated with A beta Ps exhibit morphological and biochemical characteristics of apoptosis, including membrane blebbing, compaction of nuclear chromatin, and internucleosomal DNA fragmentation. Aurintricarboxylic acid, an inhibitor of nucleases, prevents DNA fragmentation and delays cell death. Our in vitro results suggest that apoptosis may play a role in the neuronal loss associated with Alzheimer disease.