gebhart/patrepeval huggingface dataset:
Low T/sub c/ superconductive circuits fabricated on 150-mm-diameter wafers using a doubly planarized Nb/AlO/sub x//Nb process (1999)

We have used a doubly planarized all-refractory technology for superconductive electronics (DPARTS) process to fabricate mixed-signal circuits that have more than 200 junctions per circuit and operate at 2 GHz. A 150-mm-diameter wafer can produce more than 400 chips, each 5 mm on an edge. The junctions had a critical current density of 1.7 kA/cm/sup 2/. The wafers were evaluated at room temperature, both in- and post-process. In-process testing was used to detect parameter shifts during processing, while post-process testing used an automated testing station to test more than 3500 structures across each completed wafer and thus establish a large set of statistical data for studying the spread and targeting of parameter values. The circuits were fabricated in a class-10 clean room in which 0.25 /spl mu/m CMOS and CCD devices were also produced. The DPARTS process could also be used for sub-/spl mu/m fabrication, as it includes optical lithography with an i-line stepper; chemical-mechanical planarization at two levels; a self-aligned via process; and dry, anisotropic etching for all metal etching and via definition steps. The use of 150-mm-diameter wafers ensures that this process will be able to exploit technological advances in the standard silicon tool set as improvements become available. The results demonstrated here are a necessary precondition to yielding large volumes of superconductive electronic circuits containing devices with sub-/spl mu/m dimensions.
Universal precharging method for dc-link and flying capacitors of four-level Flying Capacitor Converter (2013)

This paper investigates universal start-up procedure for Flying Capacitor Converter (FLC). Main part of this procedure is pre-charging of the dc-link and flying capacitors. This method is usable for voltage pre-charging in rectifier as well as inverter mode of the multilevel FLC converter. This specific feature is based on use of converter topology and modification of converter switching pulses control. The proposed control improvements are designed to control particularly multilevel converters. The control system is implemented in development platform Multi Level Converter (MLC) interface for a four-level multilevel converter with flying capacitors. Its performance is investigated and evaluated by selected experiments made on a developed converter prototype of the rated power of 30 kVA. This paper also discusses behaviour of converter on occurrence of safe restart and shut-down procedure.
New cache designs for thwarting software cache-based side channel attacks (2007)

Software cache-based side channel attacks are a serious new class of threats for computers. Unlike physical side channel attacks that mostly target embedded cryptographic devices, cache-based side channel attacks can also undermine general purpose systems. The attacks are easy to perform, effective on most platforms, and do not require special instruments or excessive computation power. In recently demonstrated attacks on software implementations of ciphers like AES and RSA, the full key can be recovered by an unprivileged user program performing simple timing measurements based on cache misses.
Experience with intravenous ferric carboxymaltose in patients with iron deficiency anemia (2014)

Erythropoiesis may be limited by absolute or functional iron deficiency or when chronic inflammatory conditions lead to iron sequestration. Intravenous iron may be indicated when oral iron cannot address the deficiency. Ferric carboxymaltose (FCM) is a nondextran iron preparation recently approved in the United States for intravenous treatment of iron deficiency anemia (IDA) in adult patients with intolerance or unsatisfactory response to oral iron or with nondialysis-dependent chronic kidney disease. The full dose is two administrations of up to 750 mg separated by at least 7 days (up to 1500 mg total). FCM can be injected in 7-8 min or diluted in saline for slower infusion. The efficacy and safety of this dose was established in two prospective trials that randomized over 3500 subjects, 1775 of whom received FCM. One trial showed similar efficacy of FCM to an approved intravenous iron regimen (1000 mg of iron sucrose) in 2500 subjects with chronic kidney disease and additional cardiovascular risk factors. The other trial showed superior efficacy of FCM to oral iron in subjects with IDA due to various etiologies (e.g. gastrointestinal or uterine bleeding). In these trials, there was no significant difference between FCM and comparator with respect to an independently adjudicated composite safety endpoint, including death, myocardial infarction, or stroke. A database of 5799 subjects exposed to FCM provided a safety profile acceptable for regulatory approval. Mechanistic studies demonstrated that the transient, asymptomatic reduction in serum phosphate observed following FCM administration results from induction of fibroblast growth factor 23, which in turn induces renal phosphate excretion. An elevated hepcidin level may identify patients with IDA who will not respond to oral iron but will respond to FCM. The ability to administer FCM in two rapid injections or infusions will likely be viewed favorably by patients and healthcare providers.
Modulation of marrow stromal osteoblast adhesion on biomimetic oligo[poly(ethylene glycol) fumarate] hydrogels modified with Arg‐Gly‐Asp peptides and a poly(ethylene glycol) spacer (2002)

Novel oligo[poly(ethylene glycol) fumarate] (OPF) hydrogels functionalized with cell adhesion peptides were prepared, and the effects of incorporated peptide density and macromolecular structure of hydrogels on attachment and morphology of marrow stromal cells (MSCs) were evaluated. Poly(ethylene glycol) (PEG; number average molecular weight of 930, 2860, and 6090) was used to synthesize OPF. A model peptide, Gly-Arg-Gly-Asp (GRGD), was incorporated into OPF hydrogels after being coupled to acrylated PEG of molecular weight 3400. The increase of incorporated peptide concentration enhanced MSC attachment to OPF hydrogels of PEG of molecular weight of 930 and 2860. However, the number of attached MSCs to OPF hydrogels of PEG (molecular weight 6090) remained constant regardless of the peptide density. The length of PEG in OPF also influenced cell attachment. When 1 micromole peptide/g hydrogel was incorporated into the OPF hydrogels, the degree of cell attachment at 12 h relative to the initial seeding density was 93.9 +/- 5.9%, 64.7 +/- 8.2%, and 9.3 +/- 6.6% for OPF hydrogels prepared with PEG of molecular weights of 930, 2860, and 6090, respectively. However, the crosslinking density of hydrogels did not significantly affect cell attachment. The interaction was sequence specific, in that MSC attachment to GRGD-modified hydrogels was competitively inhibited when cells were incubated in the presence of 0.5 mM soluble GRGD before cell seeding. These results suggest that we can modulate MSC attachment to OPF hydrogels by altering the peptide density and the molecular structure of OPF hydrogels.
The Intestinal Peptide PEC-60 Inhibits Insulin Secretion in the Mouse and the Rat (1992)

The newly discovered 60-amino-acid porcine intestinal peptide, PEC-60, shows a structural similarity to pancreatic secretory trypsin inhibitor. PEC-60 was recently demonstrated to inhibit glucose-stimulated insulin secretion from the perfused rat pancreas. We examined in this study whether the peptide affects basal and stimulated insulin secretion in vivo. Purified porcine PEC-60 was injected intravenously in mice at 1 or 8 nmol/kg alone or together with glucose (2.8 mmol/kg) or the cholinergic agonist carbachol (0.16 mumol/kg). PEC-60 was found to inhibit glucose- and carbachol-induced insulin secretion (p less than 0.01 and p less than 0.05, respectively) at 8 nmol/kg, whereas at 1 nmol/kg, the peptide had no effect. In contrast, basal plasma insulin levels were not affected by PEC-60. In a second experimental series, PEC-60 was infused intravenously in rats at 17 or 68 pmol/min alone or together with glucose (56 mumol/min). At 68 pmol/min (p less than 0.01), but not at 17 pmol/min, PEC-60 inhibited glucose-stimulated insulin secretion. The peptide had no influence on basal plasma insulin levels. It is concluded that the newly isolated intestinal peptide, PEC-60, inhibits stimulated insulin secretion under in vivo conditions both in the mouse and in the rat without affecting basal insulin secretion.
Substitutions of cysteine residues of Escherichia coli heat-stable enterotoxin by oligonucleotide-directed mutagenesis (1987)

The Escherichia coli 18-amino-acid, heat-stable enterotoxin STp has six cysteine residues linked intramolecularly by three disulfide bonds. These disulfide bonds are important for toxic activity, but the precise role of each bond is not clear. We substituted cysteine residues of STp in vivo by oligonucleotide-directed site-specific mutagenesis to dissociate each disulfide bond and examined the biological activities of the resulting mutants. The Cys-6----Ala and Cys-17----Ala mutations caused a complete loss of toxic activity. The Cys-5----Ala, Cys-10----Ser, and Gly-16, Cys-17----Cys-16, Gly-17 mutations caused a large decrease in toxic activity. These results mean that all three disulfide bonds formed at fixed positions are required for full expression of the biological activity of STp. However, a weak but significant toxicity still remained after three mutations, Cys-5----Ala, Cys-10----Ser, and Gly-16, Cys-17----Cys-16, Gly-17. This indicates that STp has some flexibilities in its conformation to exert toxic activity and that the role of each disulfide bond exerting toxic activity is not quite the same.
Actions of long chain alcohols on GABA<sub>a</sub>and glutamate receptors: relation to<i>in vivo</i>effects (1996)

1. The effects of n-alcohols on GABAA and glutamate receptor systems were examined, and in vitro effectiveness was compared with in vivo effects in mice and tadpoles. We expressed GABAA, NMDA, AMPA, or kainate receptors in Xenopus oocytes and examined the actions of n-alcohols on receptor function using two-electrode voltage clamp recording. 2. The function of GABAA receptors composed of alpha 1 beta 1 or alpha 1 beta 1 gamma 2L subunits was potentiated by all of the n-alcohols studied (butanol-dodecanol). 3. In contrast to GABAA receptors, glutamate receptors expressed from mouse cortical mRNA or from cRNAs encoding AMPA (GluR3)- or kainate (GluR6)-selective subunits were much less sensitive to longer chain alcohols. In general, octanol and decanol were either without effect or high concentrations were required to produce inhibition. 4. In contrast to the lack of behavioural effects by long chain alcohols reported previously, decanol produced loss of righting reflex in short- and long-sleep mice, indicating that the in vivo effects of decanol may be due in part to actions at GABAA receptors. Furthermore, butanol, hexanol, octanol, and decanol produce similar potentiation of GABAA receptor function at concentrations required to cause loss of righting reflex in tadpoles, an in vivo model where alcohol distribution is not a compromising factor. 5. Thus, the in vivo effects of long chain alcohols are not likely to be due to their actions on NMDA, AMPA, or kainate receptors, but may be due instead to potentiation of GABAA receptor function.
Expression of deletion constructs of bovine β-1, 4-galactosyltransferase in <i>Escherichia coli</i>: importance of Cysl34 for its activity (1993)

Bovine beta-1,4-galactosyltransferase (beta-1,4-GT; EC 2.4.1.90) belongs to the glycosyltransferase family and as such shares a general topology: an N-terminal cytoplasmic tail, a signal anchor followed by a stem region and a catalytic domain at the C-terminal end of the protein. cDNA constructs of the N-terminal deleted forms of beta-1,4-GT were prepared in pGEX-2T vector and expressed in E. coli as glutathione-S-transferase (GST) fusion proteins. Recombinant proteins accumulated within inclusion bodies as insoluble aggregates that were solubilized in 5 M guanidine HCl and required an 'oxido-shuffling' reagent for regeneration of the enzyme activity. The recombinant beta-1,4-GT, devoid of the GST domain, has 30-85% of the sp. act. of bovine milk beta-1,4-GT with apparent Kms for N-acetylglucosamine and UDP-galactose similar to those of milk enzyme. Deletion analyses show that both beta-1,4-GT and lactose synthetase activities remain intact even in the absence of the first 129 residues (pGT-d129). The activities are lost when either deletions extend up to residue 142 (pGT-d142) or Cys134 is mutated to Ser (pGT-d129C134S). These results suggest that the formation of a disulfide bond involving Cys134 holds the protein in a conformation that is required for enzymatic activity.
Measuring the Effects of Chemicals on the Brain (1999)

"Measuring the Effects of Chemicals on the Brain." , 54(3), p. 150 Additional informationNotes on contributorsKaye H. KilburnRalph Edgington Professor of Medicine Los Angeles, California Editor-in-Chief Archives of Environmental Health
3D CSEM modeling and time-lapse sensitivity analysis for subsurface CO2 storage (2012)

ABSTRACT Monitoring a time-lapse geophysical anomaly due to variation in the pore-fluid contents of a subsurface reservoir is critical for further development and management. However, it may be difficult to detect the changes in P-wave responses beyond a certain level of saturation. A comprehensive 3D finite-difference time domain (FDTD) forward modeling, based on idealized subsurface resistivity structures, demonstrates the possibility of marine controlled-source electromagnetic (CSEM) surveying to monitor the movement of subsurface CO2 storage. We focused on CSEM sensitivity studies with respect to variations in geometry and/or saturation of CO2 storage, evaluation of the effects of reservoir depths and lithology, and detection of CO2 leakage from the main storage. A 100% lateral expansion from the initial diameter (1500 m) of a typical CO2 plume with thickness and resistivity of 100 m and 80 Ωm, respectively, at 850 m below seafloor, results in 41% electric field (E-field) magnitude increase at 2500 m source-receiver offset for 0.5 Hz source frequency. In comparison, a 300% vertical expansion of a 50 m thick plume with diameter and resistivity of 2500 m and 80 Ωm, respectively, gives only a 7% E-field magnitude anomaly. An increase of the aspect ratio of a CO2 plume with diameter and thickness of 1750 and 130 m, respectively, by 110%, without volumetric change, results in a 20% E-field magnitude increase. The E-field magnitude differences due to CO2 saturation change by 20% in clay-rich (10% clay) and clean reservoirs are almost identical; thus, a time-lapse anomaly in a clay-rich reservoir might be detectable. A gradual inverse variation of offset and frequency can differentiate the responses of a 50 m thick shallow CO2 accumulation, 200 m below the seafloor, from the main CO2 plume at 850 m below the seafloor and allow for early warning of CO2 leakage.
Origin of Antimony Segregation in<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mi>GaInSb</mml:mi><mml:mi>/</mml:mi><mml:mi>InAs</mml:mi></mml:math>Strained-Layer Superlattices (2000)

We show how cross-sectional scanning tunneling microscopy may be used to reconstruct the Sb segregation profiles in GaInSb /InAs strained-layer superlattices. These profiles are accurately described by a one-dimensional model parametrizing the spatial evolution of an Sb seed at the InAs-on-GaInSb interface in terms of two-anion-layer exchange. We argue that the segregation seed, which decreases from 2 / 3 to 1 / 2 monolayer when growth conditions are made less anion rich, has its origin in the Sb-bilayer reconstruction maintained during GaInSb epitaxy.
TNF downmodulates the function of human CD4+CD25hi T-regulatory cells (2006)

CD4+CD25+ T-regulatory cells (Tregs) play an essential role in maintaining immunologic homeostasis and preventing autoimmunity. However, little is known about the exogenous factors that regulate their differentiation and function. Here, we report that TNF inhibits the suppressive function of both naturally occurring CD4+CD25+ Tregs and TGFbeta1-induced CD4+CD25+ T-regulatory cells. The mechanism of this inhibition involves signaling through TNFRII that is constitutively expressed selectively on unstimulated Tregs and that is up-regulated by TNF. TNF-mediated inhibition of suppressive function is related to a decrease in FoxP3 mRNA and protein expression by the Tregs. Notably, CD4+CD25hi Tregs isolated from patients with active rheumatoid arthritis (RA) expressed reduced levels of FoxP3 mRNA and protein and poorly suppressed the proliferation and cytokine secretion of CD4+ effector T cells in vitro. Treatment with anti-TNF antibody (infliximab) increased FOXP3 mRNA and protein expression by CD4+CD25hi Tregs and restored their suppressive function. Thus, TNF has a novel action in modulating autoimmunity, by inhibiting CD4+CD25+ Treg activity.
Overexpression of DNA replication and repair enzymes in cisplatin-resistant human colon carcinoma HCT8 cells and circumvention by azidothymidine. (1989)

Biochemical differences were demonstrated between two cell lines derived from a human colon carcinoma (HCT8), one sensitive (HCT8S), and one 4.3-fold resistant to cisplatin (HCT8DDP). The cisplatin-resistant cell line overexpressed five enzymes (dihydrofolate reductase, thymidine 5'-monophosphate synthase, thymidine kinase, and DNA polymerase alpha and beta) believed to be important for DNA replicative and repair synthesis. In addition, the c-fos and c-H-ras oncogenes were also overexpressed in the HCT8DDP cells. This apparent overexpression was not associated with increases in gene copy number, it was related, however, to increased mRNA content. Expression of these key enzymes may be a significant factor in the development of clinical resistance to cisplatin. Further, these specific changes in cellular metabolism associated with cisplatin resistance may be exploited by the use of nucleoside analogues.
Femtosecond laser melting of graphite (1989)

We report the first femtosecond time-resolved measurements of melting dynamics of graphite. A high-reflectivity phase, lasting less than 10 ps, appears when and only when the surface is photoexcited above a critical fluence of 0.13 J/${\mathrm{cm}}^{2}$ needed to produce surface damage. The wavelength, polarization, and fluence dependence of time-resolved reflectivity suggest formation of an initial monovalent metallic phase, which transforms within 30 ps to a low-reflectivity phase, similar to that observed in picosecond reflectivity experiments.
DNA Microarray to Monitor the Expression of MAGE-A Genes (2002)

This post-PCR microarray assay could be useful to evaluate MAGE expression in tumors before therapeutic vaccinations with MAGE-A gene products.
Chemical derivatization of microelectrode arrays by oxidation of pyrrole and N-methylpyrrole: fabrication of molecule-based electronic devices (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical derivatization of microelectrode arrays by oxidation of pyrrole and N-methylpyrrole: fabrication of molecule-based electronic devicesGregg P. Kittlesen, Henry S. White, and Mark S. WrightonCite this: J. Am. Chem. Soc. 1984, 106, 24, 7389–7396Publication Date (Print):November 1, 1984Publication History Published online1 May 2002Published inissue 1 November 1984https://pubs.acs.org/doi/10.1021/ja00336a016https://doi.org/10.1021/ja00336a016research-articleACS PublicationsRequest reuse permissionsArticle Views1481Altmetric-Citations320LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Monitoring Epidemic Alert Levels by Analyzing Internet Search Volume (2012)

The prevention of infectious diseases is a global health priority area. The early detection of possible epidemics is the first and important defense line against infectious diseases. However, conventional surveillance systems, e.g., the Centers for Disease Control and Prevention (CDC), rely on clinical data. The CDC publishes the surveillance results weeks after epidemic outbreaks. To improve the early detection of epidemic outbreaks, we designed a syndromic surveillance system to predict the epidemic trends based on disease-related Google search volume. Specifically, we first represented the epidemic trend with multiple alert levels to reduce the noise level. Then, we predicted the epidemic alert levels using a continuous density HMM, which incorporated the intrinsic characteristic of the disease transmission for alert level estimation. Respective models are built to monitor both national and regional epidemic alert levels of the U.S. The proposed system can provide real-time surveillance results, which are weeks before the CDC's reports. This paper focusses on monitoring the infectious disease in the U.S., however, we believe similar approach may be used to monitor epidemics for the developing countries as well.
BRITE: an approach to universal topology generation (2002)

Effective engineering of the Internet is predicated upon a detailed understanding of issues such as the large-scale structure of its underlying physical topology, the manner in which it evolves over time, and the way in which its constituent components contribute to its overall function. Unfortunately, developing a deep understanding of these issues has proven to be a challenging task, since it in turn involves solving difficult problems such as mapping the actual topology, characterizing it, and developing models that capture its emergent behavior. Consequently, even though there are a number of topology models, it is an open question as to how representative the generated topologies they generate are of the actual Internet. Our goal is to produce a topology generation framework which improves the state of the art and is based on the design principles of representativeness, inclusiveness, and interoperability. Representativeness leads to synthetic topologies that accurately reflect many aspects of the actual Internet topology (e.g. hierarchical structure, node degree distribution, etc.). Inclusiveness combines the strengths of as many generation models as possible in a single generation tool. Interoperability provides interfaces to widely-used simulation applications such as ns and SSF and visualization tools like otter. We call such a tool a universal topology generator.