gebhart/patrepeval huggingface dataset:
The role of phospholipid and factor VIIIa in the activation of bovine factor X. (1981)

The kinetic parameters of bovine factor X activation by bovine factor IXa have been determined in the absence and presence of Ca2+, thrombin-activated bovine factor VIII (VIIIa), and phospholipid (dioleoylphosphatidylcholine/dioleoylphosphatidylserine, 75/25; mol/mol). Factor IXa in the absence of Ca2+, factor VIIIa, and phospholipid is able to catalyze factor X activation. The Km for factor X is 299 microM which is well above its concentration in bovine plasma, about 0.2 microM. The Vmax of factor Xa formation is 0.0022 mol of Xa . min-1 . mol of IXa-1 under these conditions. Addition of Ca2+ has little effect on the kinetic constants of factor X activation by factor IXa. In the presence of 10 mM CaCl2 the Km for factor X is 181 microM, and the Vmax is 0.0105 mol of Xa . min-1 . mol of IXa-1. The presence of 10 microM phospholipid dramatically decreases the Km for factor X to 0.058 microM, and the Vmax becomes 0.0025 mol of Xa . min-1 . mol of IXa-1. The Vmax of factor Xa formation slightly increases when more phospholipid is present in our experiments, and there is a considerable increase of the Km for factor X at higher phospholipid concentrations. Therefore, the Km measured in the presence of phospholipid has to be regarded as an apparent Km. The possible explanations for this phenomenon are discussed. For the complete factor X-activating complex (i.e. factor IXa, factor VIIIa, Ca2+, and 10 microM phospholipid) the Km for factor X is 0.0063 microM, and the Vmax is raised 200,000-fold to 500 mol of Xa . min-1 . mol of IXa-1. In order to exert its stimulating effect on factor X activation factor VIII has to be activated with thrombin. Our results show that factor IXa is an enzyme which can activate factor X at a very low rate. The stimulating effect of phospholipid in factor X activation is mainly due to an effect on the Km for factor X, bringing it within the range of the plasma concentration. The stimulatory effect of factor VIIIa is explained by its 200,000-fold increase of the Vmax of factor Xa formation.
Statistical theory for the equilibrium distribution of rigid molecules in inert porous networks. Exclusion chromatography (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStatistical theory for the equilibrium distribution of rigid molecules in inert porous networks. Exclusion chromatographyJ. Calvin Giddings, Eugene Kucera, Christopher Penn Russell, and Marcus N. MyersCite this: J. Phys. Chem. 1968, 72, 13, 4397–4408Publication Date (Print):December 1, 1968Publication History Published online1 May 2002Published inissue 1 December 1968https://doi.org/10.1021/j100859a008RIGHTS & PERMISSIONSArticle Views1199Altmetric-Citations382LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alerts
Effect of ustekinumab on physical function and health-related quality of life in patients with psoriatic arthritis: a randomized, placebo-controlled, phase II trial (2010)

Ustekinumab significantly improved physical function and HRQoL in patients with PsA and psoriasis involving at least 3% BSA.
( <sup>111</sup> ) INDIUM-CAPROMAB PENDETIDE UNEXPECTEDLY LOCALIZES TO RENAL CELL CARCINOMA (1999)

No AccessJournal of UrologyClinical Urology: Case Reports1 Feb 1999(111) INDIUM-CAPROMAB PENDETIDE UNEXPECTEDLY LOCALIZES TO RENAL CELL CARCINOMA ELI K. MICHAELS, MICHAEL BLEND, and JUAN CARLOS QUINTANA ELI K. MICHAELSELI K. MICHAELS More articles by this author , MICHAEL BLENDMICHAEL BLEND More articles by this author , and JUAN CARLOS QUINTANAJUAN CARLOS QUINTANA More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)61960-8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "(111) INDIUM-CAPROMAB PENDETIDE UNEXPECTEDLY LOCALIZES TO RENAL CELL CARCINOMA." The Journal of Urology, 161(2), pp. 597–598 References 1 : The detection of metastatic prostate cancer with simultaneous dual isotope SPECT images. RSNA. ORG/EJ.September 11, 1997; vol. 1. Google Scholar 2 : Risk of genitourinary malignancies after cancer of the prostate. Cancer1988; 61: 396. Google Scholar 3 : Monoclonal antibodies to the extracellular domain of prostate-specific membrane antigen also react with tumor vascular endothelium. Cancer Res.1997; 9: 3629. Google Scholar From the Departments of Urology and Radiology, Section of Nuclear Medicine, University of Illinois at Chicago, Chicago, IllinoisSupported by a grant from the Cytogen Corporation.© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySCOTT D, HALKAR R, FISCHER A, GALT J and CLARKE H (2018) FALSE-POSITIVE 111INDIUM CAPROMAB PENDETIDE SCAN DUE TO BENIGN MYELOLIPOMAJournal of Urology, VOL. 165, NO. 3, (910-911), Online publication date: 1-Mar-2001. Volume 161Issue 2February 1999Page: 597-598 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.MetricsAuthor Information ELI K. MICHAELS More articles by this author MICHAEL BLEND More articles by this author JUAN CARLOS QUINTANA More articles by this author Expand All Advertisement PDF downloadLoading ...
A Collaborative UAV-WSN Network for Monitoring Large Areas (2018)

Large-scale monitoring systems have seen rapid development in recent years. Wireless sensor networks (WSN), composed of thousands of sensing, computing and communication nodes, form the backbone of such systems. Integration with unmanned aerial vehicles (UAVs) leads to increased monitoring area and to better overall performance. This paper presents a hybrid UAV-WSN network which is self-configured to improve the acquisition of environmental data across large areas. A prime objective and novelty of the heterogeneous multi-agent scheme proposed here is the optimal generation of reference trajectories, parameterized after inter- and intra-line distances. The main contribution is the trajectory design, optimized to avoid interdicted regions, to pass near predefined way-points, with guaranteed communication time, and to minimize total path length. Mixed-integer description is employed into the associated constrained optimization problem. The second novelty is the sensor localization and clustering method for optimal ground coverage taking into account the communication information between UAV and a subset of ground sensors (i.e., the cluster heads). Results show improvements in both network and data collection efficiency metrics by implementing the proposed algorithms. These are initially evaluated by means of simulation and then validated on a realistic WSN-UAV test-bed, thus bringing significant practical value.
MicroRNA-26b inhibits epithelial-mesenchymal transition in hepatocellular carcinoma by targeting USP9X (2014)

Taken together, our results indicate that miR-26b were inhibited in HCC. In HCC cell lines, miR-26b targeted the 3'UTR of USP9X, which in turn affects EMT through Smad4 and the TGF-β signaling pathway. Our analysis of clinical HCC samples verifies that miR-26b also targets USP9X expression to inhibit the EMT of hepatocytes. Thus, miR-26b may have some effects on the EMT of HCC cells.
Non-Heme Fe(IV)–Oxo Intermediates (2007)

High-valent non-heme iron-oxo intermediates have been proposed for decades as the key intermediates in numerous biological oxidation reactions. In the past three years, the first direct characterization of such intermediates has been provided by studies of several alphaKG-dependent oxygenases that catalyze either hydroxylation or halogenation of their substrates. In each case, the Fe(IV)-oxo intermediate is implicated in cleavage of the aliphatic C-H bond to initiate hydroxylation or halogenation. The observation of non-heme Fe(IV)-oxo intermediates and Fe(II)-containing product(s) complexes with almost identical spectroscopic parameters in the reactions of two distantly related alphaKG-dependent hydroxylases suggests that members of this subfamily follow a conserved mechanism for substrate hydroxylation. In contrast, for the alphaKG-dependent non-heme iron halogenase, CytC3, two distinct Fe(IV) complexes form and decay together, suggesting that they are in rapid equilibrium. The existence of two distinct conformers of the Fe site may be the key factor accounting for the divergence of the halogenase reaction from the more usual hydroxylation pathway after C-H bond cleavage. Distinct transformations catalyzed by other mononuclear non-heme enzymes are likely also to involve initial C-H bond cleavage by Fe(IV)-oxo complexes, followed by diverging reactivities of the resulting Fe(III)-hydroxo/substrate radical intermediates.
Individual, but not simultaneous, glucagon and cholecystokinin infusions inhibit feeding in men (1992)

Pancreatic glucagon and cholecystokinin octapeptide (CCK-8) were intravenously infused (1 ml/min for 10 min) alone or in combination beginning 15 min after normal-weight men had eaten a 500-ml tomato soup preload and 5 min before they were served a lunch of macaroni and beef with tomato sauce. Infusion of approximately 3 ng.kg-1.min-1 glucagon or approximately 2 ng.kg-1.min-1 CCK-8 each reduced test meal size. However, simultaneous infusion of these peptide doses reduced meal size less than the sum of the peptides' individual effects. Infusions of approximately 1.5 ng.kg-1.min-1 glucagon or approximately 1 ng.kg-1.min-1 CCK-8 had neither individual nor interactive effects on meal size. Psychophysical ratings failed to detect nonspecific side effects after any of the infusions. That exogenous glucagon and CCK-8 each reduced meal size without side effects suggests that these peptides may participate in the physiological control of human appetite; that their simultaneous infusion resulted in an infra-additive reduction in meal size suggests that they can interact antagonistically.
Development of dyskinesias in a 5‐year trial of ropinirole and <scp>L</scp>‐dopa (2006)

A 5-year trial of ropinirole and levodopa in early Parkinson's disease showed that ropinirole is associated with reduced incidence of dyskinesias. This post hoc analysis investigated whether the dyskinesia-sparing benefit of ropinirole is lost when levodopa is added to the regimen and evaluated other risk factors for developing dyskinesias. Patients receiving levodopa had a significantly higher risk of dyskinesias than those taking ropinirole monotherapy (hazard ratio [HR], 6.67; 95% confidence interval [CI], 3.23-14.29; P < 0.001). When patients randomized to ropinirole were treated with supplementary levodopa, the development of dyskinesias was not significantly different from that in those receiving levodopa from the start (HR, 0.80; 95% CI, 0.48-1.33; P = 0.39). However, the onset of dyskinesias was delayed by around 3 years compared with levodopa monotherapy. Adjusted analyses taking into account baseline and on-treatment factors that influenced use of supplementary levodopa or the development of dyskinesias produced similar results. In conclusion, the risk of developing dyskinesias during maintained initial ropinirole monotherapy is very low. Only once levodopa is added does the risk substantially change. Early use of ropinirole postpones the onset of dyskinesias, but these benefits decline when levodopa therapy is started, with no evidence of a subsequent rapid "catch-up" or a persisting preventive effect.
Common subtypes of idiopathic generalized epilepsies: Lack of linkage to D20S19 close to candidate loci (EBN1, EEGV1) on chromosome 20 (1996)

Hereditary factors play a major role in the etiology of idiopathic generalized epilepsies (IGEs). A trait locus (EBN1) for a rare subtype of IGEs, the benign neonatal familial convulsions, and a susceptibility gene (EEGV1) for the common human low-voltage electroencephalogram have been mapped close together with D20S19 to the chromosomal region 20q13.2. Both loci are potential candidates for the susceptibility to IGE spectra with age-related onset beyond the neonatal period. The present study tested the hypothesis that a putative susceptibility locus linked to D20S19 predisposes to spectra of IGEs with age-related onset from childhood to adolescence. Linkage analyses were conducted in 60 families ascertained through IGE patients with juvenile myoclonic epilepsy, juvenile absence epilepsy or childhood absence epilepsy. Our results provide evidence against linkage of a putative susceptibility gene for four hierarchically broadened IGE spectra with D20S19 assuming tentative single-locus genetic models. The extent of an "exclusion region" (lod scores below-2) varied from 0.5 cM up to 22 cM on either side of D20S19 depending on the trait assumed. These results are contrary to the expectation that a susceptibility gene in vicinity to D20S19 confers a common major gene effect to the expression of IGE spectra with age-related onset from childhood to adolescence.
Insecticidal Activity of the Toxins from Bacillus thuringiensis subspecies kurstaki and tenebrionis Adsorbed and Bound on Pure and Soil Clays (1995)

The release of transgenic plants and microorganisms expressing truncated genes from various subspecies of Bacillus thuringiensis that encode active insecticidal toxins rather than inactive protoxins could result in the accumulation of these active proteins in soil, especially when bound on clays and other soil particles. Toxins from B. thuringiensis subsp. kurstaki and B. thuringiensis subsp. tenebrionis, either free or adsorbed at equilibrium or bound on pure clay minerals (montmorillonite or kaolinite) or on the clay size fraction of soil, were toxic to larvae of the tobacco hornworm (Manduca sexta) and the Colorado potato beetle (Leptinotarsa decemlineata), respectively. The 50% lethal concentrations (LC(inf50)) of free toxins from B. thuringiensis subsp. kurstaki were higher than those of both bound and adsorbed complexes of these toxins with clays, indicating that adsorption and binding of these toxins on clays increase their toxicity in diet bioassays. The LC(inf50) of the toxin from B. thuringiensis subsp. tenebrionis that was either free or adsorbed on montmorillonite were comparable, whereas the toxin bound on this clay had higher LC(inf50) and the toxin bound on kaolinite had lower LC(inf50) than when adsorbed on this clay. Results obtained with the clay size fraction separated from unamended soil or soil amended with montmorillonite or kaolinite were similar to those obtained with the respective pure clay minerals. Therefore, insecticidal activity of these toxins is retained and sometimes enhanced by adsorption and binding on clays.
The complete solution for third-generation wireless communications: two modes on air, one winning strategy (2000)

Due to the increasing number of users in mobile communications and the demand for mobile multimedia services with high data rates, third-generation mobile radio systems are currently one of the key communication technologies in research, development, and international standardization bodies. This article summarizes the international standardization activities for this new technology, outlines its key applications, and provides an overview of UMTS as specified by the 3GPP. Moreover, an overview of capacity-enhancing features such as joint (multi-user) detection and smart antennas is given. Finally, we develop a vision for mobile communications beyond the third generation.
Role of energy metabolism in Mycoplasma pneumoniae attachment to glass surfaces (1981)

Attachment values of Mycoplasma pneumoniae to glass are normally very low when tested in buffer containing bovine serum albumin (10 mg/ml). However, the addition of one of the metabolizable sugars glucose, fructose, or mannose increased attachment more than 10-fold. The effect was dose dependent with a distinct optimum at about 0.25 mg/ml. Higher concentrations reduced this effect. Not only the sugars themselves but also the products of their catabolism, pyruvate and phosphoenolpyruvate, enhanced attachment. Pyruvate was effective in the same range of concentrations as the sugars, whereas phosphoenolpyruvate enhanced attachment at a significantly lower concentration (0.001 mg/ml). Higher levels of these substances also resulted in a decrease of attachment. The glucose-induced increase could be partially inhibited by glucose analogs, especially by 3-O-methyl-glucopyranoside, and by various inhibitors or glycolysis. Furthermore, attachment was strongly reduced by the uncoupling agents carbonylcyanide m-chlorophenylhydrazone and 2,4-dinitrophenol, as well as by dicyclohexylcarbodiimide, an inhibitor of the membrane-bound Mg2+-adenosine triphosphatase, whereas the ionophore valinomycin increased attachment by about 30%. These findings provide strong evidence for coupling between the attachment process of M. pneumoniae to glass and the utilization of metabolic energy.
Perfusion CT of the brain: diagnostic approach for early detection of ischemic stroke. (1998)

Perfusion CT not only allows early detection of cerebral ischemia but also yields valuable information about the extent of perfusion disturbances.
Amphotericin B and the Blood-Brain Barrier to Methotrexate (1982)

The permeability of the blood-brain barrier (BBB) to methotrexate, Na+, and Cl− was studied in rats treated with the membrane-active antifungal agent amphotericin B. Enhancement of brain uptake of methotrexate was expected as amphotericin B increases the permeability of lipid membranes, including tumor cell membranes. However, amphotericin B had no effect on the BBB permeability to methotrexate, Na+, and Cl−. This may have been because the amphotericin B acted only on the luminal and not on the abluminal brain capillary membrane, leaving the BBB intact. (Neurosurgery 10:224-226, 1982)
Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivo. (2003)

Many leukemias result from chromosomal translocations that lead to the generation of chimeric oncoproteins. Chimeric gene for the promyelocytic leukemia-retinoic acid receptor alpha (PML-RAR alpha) is the hallmark of acute promyelocytic leukemia. Specific gene silencing of an oncogene is desirable for the treatment of these diseases. We have previously constructed an allosterically controllable ribozyme (designated maxizyme) targeted for bcr-abl chimeric mRNA, whose cleavage activity is induced only in the presence of a specific RNA sequence of interest. It has been demonstrated recently that RNA interference induced by short hairpin-type RNAs provides a powerful method for sequence-specific gene silencing. Here we report that DNA vector-based maxizymes and short hairpin-type RNAs driven by the promoter of a human gene for tRNA(Val) specifically inhibit the expression of the chimeric gene for PML-RAR alpha both in vitro and in vivo. Our findings confirm the potential utility of maxizymes and shRNAs as therapeutic agents.
Clinical importance of thrombomodulin serum levels. (2001)

Thrombomodulin is a glycoprotein that can bind to thrombin and activate protein C, thus mitigating the effects of cytokines produced by inflammatory and immunological processes. The molecule exerts a protective function on endothelial cells. Thrombomodulin is cleaved to its soluble form by neutrophil elastase and by other substances produced during acute and chronic inflammatory responses, immunologic reactions and complement activation. ELISA technique yields normal serum levels of 3.1 +/- 1.3 ng/ml; in males these levels are higher; TM levels also rise during menopause. Other circumstances associated with an increase of serum TM levels are smoking, disseminated intravascular coagulation (DIC), cardiac surgery, atherosclerosis, ARDS, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis. Serum levels of TM represent an useful prognostic index, because they are associated with an increase in mortality rate, or however a progression of the underlying pathological condition.
Fat-density oral contrast agent for abdominal CT. (1987)

To overcome the problem of inadequate mixing of gastrointestinal (GI) tract contents with conventional high-density oral contrast agents in abdominal computed tomography (CT), a flavored 12.5% corn oil emulsion (COE) was tested as an oral contrast agent in 62 patients. The results were compared with those obtained from 105 patients who received high-density oral contrast agents. A simple patient preparation regimen for the COE was developed, which appears to be reasonably well tolerated by the patients and yields diagnostic studies comparable to those obtained with conventional agents. Use of the COE resulted in consistent discrimination of the GI tract and depiction of the GI wall. Preliminary data suggest that it may be particularly useful for CT evaluation of the stomach, duodenum, and pancreas; in patients suspected of having solid tumors; and in thin people.
1,3‐Dipolar Cycloadditions. Past and Future (1963)

Abstract In contrast to the very large number of special methods applicable to syntheses in the heterocyclic series, relatively few general methods are available. The 1,3‐dipolar addition offers a remarkably wide range of utility in the synthesis of five‐membered heterocycles. Here the “1,3‐dipole”, which can only be represented by zwitterionic octet resonance structures, combines in a cycloaddition with a multiple bond system – the “dipolarophile” – to form an uncharged five‐membered ring. Although numerous individual examples of this reaction were known, some even back in the nineteenth century, fruitful development of this synthetic principle has been achieved only in recent years.
Roles for miRNAs in endocrine resistance in breast cancer (2015)

Therapies targeting estrogen receptor alpha (ERα), including selective ER modulators such as tamoxifen, selective ER downregulators such as fulvestrant (ICI 182 780), and aromatase inhibitors such as letrozole, are successfully used in treating breast cancer patients whose initial tumor expresses ERα. Unfortunately, the effectiveness of endocrine therapies is limited by acquired resistance. The role of microRNAs (miRNAs) in the progression of endocrine-resistant breast cancer is of keen interest in developing biomarkers and therapies to counter metastatic disease. This review focuses on miRNAs implicated as disruptors of antiestrogen therapies, their bona fide gene targets and associated pathways promoting endocrine resistance.