gebhart/patrepeval huggingface dataset:
Lipoprotein Lipase Association with Lipoproteins Involves Protein-Protein Interaction with Apolipoprotein B (1995)

Lipoprotein lipase (LPL) hydrolyzes chylomicron and very low density lipoprotein (VLDL) triglycerides and potentiates the cellular uptake of lipoproteins. These LPL-lipoprotein associations could involve only protein-lipid interaction, or they could be modulated by apolipoproteins (apo). ApoB is the major protein component of chylomicrons, VLDL, and low density lipoprotein (LDL). ApoB100, a large glycoprotein with a molecular mass of 550 kDa, is composed of several functional domains. A carboxyl-terminal region of the protein is the ligand for the LDL receptor. There are several hydrophobic domains that are believed to be important in lipid binding. The relatively hydrophilic amino-terminal region of apoB, however, has no known function. Using solid phase assays we quantified LPL-lipoprotein complex formation. On a molar basis, severalfold greater amounts of LPL bound to LDL and VLDL than to high density lipoprotein at all the concentrations of LPL tested (0.9-55 nM). To assess the roles of LDL protein versus lipid, we performed competition and ligand blotting experiments. LDL and an amino-terminal fragment of apoB competed better for 125I-LPL binding to LDL than did lipid emulsion particles. Delipidation of LDL-coated plates did not alter LPL binding. On ligand blots, LPL bound to amino-terminal fragments of apoB generated by thrombin digestion but not to apoA1, apoE, or carboxyl-terminal fragments of apoB. Further evidence for LPL interaction with the amino-terminal region of apoB was obtained using anti-apoB monoclonal antibodies. Antibodies directed against the amino-terminal regions of apoB blocked LPL interaction with LDL, whereas those against the carboxyl-terminal region of apoB did not inhibit LPL interaction with LDL. Thus, we conclude that a specific interaction between LPL and the amino-terminal region of apoB may facilitate LPL association with circulating lipoproteins.
Sharing buffer pool memory in multi-tenant relational database-as-a-service (2015)

Relational database-as-a-service (DaaS) providers need to rely on multi-tenancy and resource sharing among tenants, since statically reserving resources for a tenant is not cost effective. A major consequence of resource sharing is that the performance of one tenant can be adversely affected by resource demands of other co-located tenants. One such resource that is essential for good performance of a tenant's workload is buffer pool memory. In this paper, we study the problem of how to effectively share buffer pool memory in multi-tenant relational DaaS. We first develop an SLA framework that defines and enforces accountability of the service provider to the tenant even when buffer pool memory is not statically reserved on behalf of the tenant. Next, we present a novel buffer pool page replacement algorithm (MT-LRU) that builds upon theoretical concepts from weighted online caching, and is designed for multi-tenant scenarios involving SLAs and overbooking. MT-LRU generalizes the LRU-K algorithm which is commonly used in relational database systems. We have prototyped our techniques inside a commercial DaaS engine and extensive experiments demonstrate the effectiveness of our solution.
A family of bioreducible poly(disulfide amine)s for gene delivery (2009)

A family of bioreducible poly(disulfide amine)s, which differ in the length of polymethylene spacer [-(CH(2))(n)-] in the main chain and the side chain, has been synthesized. These bioreducible poly(disulfide amine)s exhibit local environment specific degradability and are associated with lower cytotoxicity than branched poly(ethylenimine) (bPEI, 25 kDa). These cationic polymers also show higher buffering capacity and protonation degree than bPEI, facilitating the endosomal escape of carried genetic materials. The transfection efficiency of these agents is oligomethylene length dependent. Poly(cystaminebisacrylamide-spermine) [poly(CBA-SP)], poly(cystaminebisacrylamide-bis(3-aminopropyl)-1,3-propanediamine) [poly(CBA-APPD)], and poly(cyxtaminebisacrylamide-bis(3-aminopropyl)-ethylenediamine) [ploy(CBA-APED)] with longer propylene [-(CH(2))(3)-] side spacer, demonstrate higher transfection efficacy than the counterpart poly(cystaminebisacrylamide-bis(2-aminoethyl)-1,3-propanediamine) [poly(CBA-AEPD)] and poly(cystaminebisacrylamide-triethylenetetramine) [poly(CBA-TETA)], which have shorter ethylene [-(CH(2))(2)-] side spacer. The poly(CBA-SP), poly(CBA-APPD), poly(CBA-APED) with the main chain spacer of -(CH(2))(4)-, -(CH(2))(3)-, -(CH(2))(2)- demonstrate similar transfection efficiency, indicating the length of polymer main chain spacer has less influence on transfection efficiency. However, with the same short ethylene [-(CH(2))(2)-] side spacer, poly(CBA-AEPD), with the longer main chain oligomethylene units [-(CH(2))(3)-], showed relatively higher transfection efficiency than poly(CBA-TETA), having shorter main chain oligomethylene units [-(CH(2))(2)-]. Of these polymeric carriers, poly(CBA-SP) demonstrated the highest transfection in the C2C12 cell line, while poly(CBA-APED) showed the highest transfection in the HeLa cell line. All of these agents showed greater transfection activity than commercialized bPEI 25 kDa. The poly(disulfide amine)s are promising safe and efficient non-viral vectors for gene delivery.
Gold Nanoparticle Networks with Photoresponsive Interparticle Spacings (2005)

Photoresponsive gold nanoparticle networks were prepared by functionalizing them with azobenzene derivatives. A network can be formed when a linker molecule constituting the azobenzene moiety suitably derivatized on either side with gold surface sensitive groups such as thiols and amines is added to the nanoparticle solution. It is shown that the interparticle spacing in the networks could be controlled by the reversible trans-cis isomerization of the azobenzene moiety induced by UV and visible light, respectively. The photoinduced variation in the interparticle spacings is inferred by the changes in the optical spectra of the gold nanoparticles which display a red or blue shift in the surface plasmon resonance peak depending on a decrease or increase in the interparticle spacing, respectively. Transmission electron microscopy images are in consonance with the evidence from the optical spectra.
BMS-936564/MDX-1338: A Fully Human Anti-CXCR4 Antibody Induces Apoptosis <i>In Vitro</i> and Shows Antitumor Activity <i>In Vivo</i> in Hematologic Malignancies (2012)

BMS-936564/MDX-1338 is a potent CXCR4 antagonist which is efficacious as monotherapy in tumor-bearing mice and is currently in phase I for the treatment of relapsed/refractory AML, NHL, CLL, and multiple myeloma.
Applications of space-time duality (2017)

Thesis (Ph. D.)--University of Rochester. Institute of Optics, 2017.
A macroblock level adaptive search range algorithm for variable block size motion estimation in H.264/AVC (2007)

Compared with previous video standards, the computational complexity of H.264/AVC is extraordinarily high due to newly adopted tools such as variable block size motion estimation (VBSME). In VBSME, to each macroblock (MB) there are at all 41 blocks corresponded. These 41 blocks are sized from 4x4 through 16x16. As each block of these 41 blocks accounts for one independent search window (SW), thus to each MB there exist 41 SWs corresponded. In the other hand, adaptive search range (ASR) algorithms are defined as algorithms which adaptively adjust the search ranges (SRs) and hence result in dynamically reduced SWs. While considering whether 41 SWs got changed in the same way or not, "macroblock level ASR (MB-ASR) algorithms" are defined as algorithms which adaptively change the search range all- in-once and hence result in the 41 SWs got changed in the same way. "41 blocks level ASR (block-ASR) algorithms" are defined as algorithms that the changing ways of the SRs are specific to each block of the 41 blocks. Since in MB-ASR algorithms for each MB the SR is changed in same way, it is reasonable to consider that the control overhead of MB-ASR is less than that of block-ASR. In this paper, a MB-ASR algorithm is proposed and assessed from being compared with a previously proposed block-ASR algorithm. According to experimental results, it is proved the proposed algorithm provides almost the same encoding quality and even a little better SW reducing efficiency (SW-RE). Meanwhile, as a MB-ASR one, the proposed algorithm is considered having less control overhead than block-ASR one.
Communication scheduling in a distributed memory parallel interactive continuous media server system (2002)

The paper describes the design of a scaleable interactive continuous media server that is realized using a distributed memory parallel computer architectures. We present a scheme for data layout, admission control and communication scheduling that provides inherent Quality of Service support for the delivery of continuous media streams from the storage devices to the external communication interfaces within the internal communication network of the media server. The methods presented here are used for the development of a parallel continuous media server that is integrated with different applications and supports the RTP/RTCP protocol for data delivery as well as the RTSP protocol to control the delivery of media streams.
Efficient Construction of a Recombinant Adenovirus Vector by an Improved <i>In Vitro</i> Ligation Method (1998)

An efficient method for constructing a recombinant adenovirus (Ad) vector, based on an in vitro ligation, has been developed. To insert the foreign gene into an adenoviral DNA, we introduced three unique restriction sites, I-CeuI, SwaI, and PI-SceI, into the E1 deletion site of the vector plasmid, which contains a complete E1, E3-deleted adenovirus type 5 genome. I-CeuI and PI-SceI are intron-encoded endonucleases with a sequence specificity of at least 9-10 and 11 bp, respectively. A shuttle plasmid, pHM3, containing multiple cloning sites between the I-CeuI and PI-SceI sites, was constructed. After the gene of interest was inserted into this shuttle plasmid, the plasmid for E1-deleted adenovirus vector could be easily prepared by in vitro ligation using the I-CeuI and PI-SceI sites. SwaI digestion of the ligation products prevented the production of a plasmid containing a parental adenovirus genome (null vector). After transformation into E. coli, more than 90% of the transformants had the correct insert. To make the vector, a PacI-digested, linearized plasmid was transfected into 293 cells, resulting in a homogeneous population of recombinant virus. The large number and strategic location of the unique restriction sites will not only increase the rapidity of production of new first-generation vectors for gene transfer but will allow for rapid further improvements in the vector DNA backbone.
Spatially Resolved Mineral Deposition on Patterned Self-Assembled Monolayers (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpatially Resolved Mineral Deposition on Patterned Self-Assembled MonolayersPeter C. Rieke, Barbara J. Tarasevich, Laurie L. Wood, Mark H. Engelhard, Donald R. Baer, Glen E. Fryxell, Connie M. John, David A. Laken, and Milt C. JaehnigCite this: Langmuir 1994, 10, 3, 619–622Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March 1994https://pubs.acs.org/doi/10.1021/la00015a003https://doi.org/10.1021/la00015a003research-articleACS PublicationsRequest reuse permissionsArticle Views234Altmetric-Citations83LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
cAMP-dependent protein kinases I and II: divergent turnover of subunits (1986)

cAMP-dependent protein kinase subunits were isolated from livers of rats that had been subjected to biosynthetic labeling with radioactive leucine. By application of ligand and antibody affinity techniques pure regulatory (R I; R II) and catalytic (C) subunits could be obtained in high yields, which allowed measurement of the apparent degradation rate constants and half-lives following a double isotope labeling protocol. In this way marked differences of apparent half-lives of regulatory subunits R I (t1/2 = 31 h) and R II (t1/2 = 125 h) were observed. To avoid the negative influence of reutilization inherent in the decay experiments, specific radioactivities were determined after a short isotope pulse. This parameter, which under steady-state conditions reflects the fractional turnover rate of the subunits, was found to be different for all three protein kinase subunits. Relative to total liver protein, the ratios R I:R II:C corresponded to 3.9:0.6:2. Our data indicate that in each type of protein kinase isoenzymes regulatory and catalytic subunits turn over with similar rates. The type I isoenzyme, however, is renewed much faster than protein kinase II. Furthermore, our findings are consistent with the thesis that free subunits as generated by activation are more susceptible to degradation than the holoenzymes, leading under steady-state conditions to compensatory resynthesis. Since renewal of R I exceeded that of R II also in two other tissues, the elevated turnover of protein kinase I as an indicator of preferential activation appears to be a general phenomenon. The different turnover of the two isoenzymes, then, may relate to different cellular functions like modulation of enzyme activity vs. modulation of gene activity.
Monitoring Usage of Workstations with a Relational Database (1994)

The ability to monitor usage of groups of workstations is quite useful for planning growth, facility hours, staffing and other issues; but in our case, both the format of the data (/var/adm/wtmp) and the fact that the data was spread over hundreds of different workstations made any analysis difficult at best.

In this paper, we explore the use of a relational database to collect all the raw data, convert it to a standard form, and then provide selection tools to extract data sets. We also examine some ways to process session data to provide more meaningful reports and charts for administrators.
A new antitumor complex, WF-1360, WF-1360A, B, C, D, E and F. (1986)

A complex of the new antitumor antibiotics (WF-1360, WF-1360A, B, C, D, E and F) was produced by Rhizopus sp. No. F-1360. Structural studies of these compounds suggested that they were novel 16-membered-ring lactones having an oxazole ring in their structures. WF-1360 was found to be identical with rhizoxin (1) and WF-1360B, C, E and F were determined to be homologues of 1 with structures 2, 3, 4 and 5, respectively. These compounds were cytotoxic when tested on P388 leukemia cells in vitro. WF-1360 was highly active against leukemia L1210 and melanoma B16. They also exhibited potent antifungal activities, but weak antimicrobial activities against some Gram-positive or negative bacteria.
Adequate oral threonine is critical for mucin production and gut function in neonatal piglets (2007)

In previous experiments, we found that the threonine requirement of neonatal piglets fed parenterally was 40% of that when fed intragastrically; we hypothesized that much of the oral supply of threonine is being used for mucin production. To investigate this hypothesis, intragastrically fed 2-day-old piglets were fed one of three treatments for 8 days: 1) a threonine-adequate diet (IG-A; 0.6 g threonine.kg(-1).day(-1) fed intragastrically); 2) a threonine-deficient diet (IG-D; 0.1 g threonine.kg(-1).day(-1) fed intragastrically); or 3) a threonine-deficient diet with adequate threonine delivered parenterally (IV-A; 0.5 g threonine.kg(-1).day(-1) fed parenterally plus 0.1 g threonine.kg(-1).day(-1) fed intragastrically). IG-D piglets experienced higher nitrogen excretion, higher plasma urea, and lower plasma threonine concentrations versus both of the other groups (P < 0.05), indicating profound threonine deficiency. Mucosal mass and total crude mucin content were lower in the colons of IG-D pigs (P < 0.05). Histopathological analysis showed lower numbers of acidic mucin-producing goblet cells in the duodenum and ileum of IG-D pigs. In IG-D pigs, acidic mucin subtypes were lower in the small intestine but higher in the colon, which corresponded with persistent diarrhea. The parenteral supply of threonine was adequate to maintain most outcome parameters, although IV-A pigs did have smaller colonic goblet cells with more acidic mucins compared with IG-A pigs. Overall, our results suggest that adequate dietary threonine was critical in the production of mucus and that a parenteral threonine supply can ameliorate most of the symptoms of oral threonine deficiency.
The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport. (1992)

Deletion of the amino-terminal 17 residues from human ADP-ribosylation factor (ARF) resulted in a protein ([delta 1-17]mARF1p) devoid of ARF activity but which retained the ability to bind guanine nucleotides with high affinity. Unlike the wild type, the binding of guanine nucleotides to this deletion mutant was found to be independent of added phospholipids. A chimeric protein was produced, consisting of 10% (the amino-terminal 17 amino acids) human ARF1p and 90% ARL1p, an ARF-like protein (55% identical protein sequence) from Drosophila. This chimera was found to have ARF activity, lacking in the parental ARL1 protein. Thus, the amino terminus of ARF1p was shown to be a critical component of ARF activity. A synthetic peptide, derived from the amino terminus of ARF1p, has no ARF activity. Rather, the peptide was found to be a specific inhibitor of ARF activities. This peptide was also found to be a potent and specific inhibitor of both an in vitro intra-Golgi transport assay and the guanosine 5'-3-O-(thio)triphosphate-stimulated accumulation of coated vesicles and buds from Golgi preparations. We conclude that ARF is required for the budding of coated vesicles from the Golgi stacks and serves a regulatory role in protein secretion through the Golgi in eukaryotic cells.
SIMPLE CONDITIONS FOR GROWTH OF UNICELLULAR BLUE‐GREEN ALGAE ON PLATES<sup>1, 2</sup> (1968)

The efficiencies of plating of 2 cultures of unicellular blue-green algae, 1 coccoid and 1 rod-shaped, were studied systematically. Reproducible colony growth and accurate viable counts are dependent on the use of a low agar concentration, and on the sterilization of the agar separately from the mineral components of the medium.
Compact antenna test ranges (1991)

Antenna test ranges are required to be capable of measuring the radiation and scattering characteristics of high performance antennas and to operate over a wide range of frequencies. The compact antenna test range (compact range) can fulfil these requirements very well and has established itself as a versatile indoor test range capable of measuring medium to large size antennas over the frequency range of 1 to 300 GHz. The compact range uses one (or more) collimating reflectors to create a region of plane waves, called the quiet zone, in front of a reflector. The paper reviews the principles, history and design of compact ranges. The various types are described and their relative advantages compared. The compact range can be used for both antenna measurements and radar cross-section measurements and shows considerable promise for further development in the future.<
 >
Serum thrombomodulin level relates to the clinical course of disseminated intravascular coagulation, multiorgan dysfunction syndrome, and mortality in patients with sepsis* (2008)

Endothelial cell injury is critical in the progression from disseminated intravascular coagulation to multiple organ dysfunction syndrome and subsequent mortality in septic patients. Serum concentrations of thrombomodulin may be used in monitoring disseminated intravascular coagulation and multiple organ dysfunction syndrome in these patients.
Plastid Genes Encoding the Transcription/Translation Apparatus Are Differentially Transcribed Early in Barley (Hordeum vulgare) Chloroplast Development (Evidence for Selective Stabilization of psbA mRNA) (1993)

Chloroplast genomes encode rRNAs, tRNAs, and proteins involved in transcription, translation, and photosynthesis. The expression of 15 plastid genes representing each of these functions was quantitated during chloroplast development in barley (Hordeum vulgare). The transcription of all plastid genes increased during the initial phase of chloroplast development and then declined during chloroplast maturation. RNAs corresponding to rpoB- rpoC1-rpoC2, which encode subunits of a plastid RNA polymerase, and rps16, which encodes a ribosomal protein, reached maximal abundance early in chloroplast development prior to genes encoding subunits of the photosynthetic apparatus (rbcL, atpB, psaA, petB). Transcription of rpoB as well as 16S rRNA, trnfM-trnG, and trnK was high early in chloroplast development and declined 10-fold relative to rbcL transcription during chloroplast maturation. RNA hybridizing to psbA and psbD, genes encoding reaction center proteins of photosystem II, was differentially maintained in mature chloroplasts of illuminated barley. Differential accumulation of psbD mRNA relative to rbcL mRNA was due to light-stimulated transcription of psbD. In contrast, enhanced levels of psbA mRNA in mature chloroplasts were due primarily to selective stabilization of the psbA mRNA. These data document dynamic modulation of plastid gene transcription and mRNA stability during barley chloroplast development.
Diagnosis and treatment of rosacea. (2002)

Rosacea is easily diagnosed by physician observation, and physicians should initiate discussion of rosacea treatment with patients. Effective treatment of rosacea includes avoidance of triggers, topical and oral antibiotic therapy, both topical and oral retinoid therapy, topical vitamin C therapy, and cosmetic surgery.