gebhart/patrepeval huggingface dataset:
Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens (2003)

In vitro cytokine profiles of peripheral blood mononuclear cells (PBMC) from pigs with postweaning multisystemic wasting syndrome (PMWS) and healthy pigs were determined in response to recall viral antigens (porcine circovirus type 2; PCV2), mitogens (phytohaemagglutinin) or superantigens (staphylococcal enterotoxin B). PBMC from PMWS-affected pigs, in contrast to those from healthy pigs, responded to recall PCV2 antigen by releasing IL-10 and IFN-gamma, but they were less able or even unable to produce IL-4, IL-2 or IFN-gamma upon challenge with mitogen or superantigen. Moreover, only PCV2 had the ability to downregulate or suppress the release of IL-4 and IL-2 from PBMC from both healthy and diseased animals, and to stimulate the production of pro-inflammatory cytokines (IL-1beta, IL-8). In conclusion, the immune system cells of PMWS pigs have a diminished ability to perform their immunological functions upon viral or immunostimulatory molecules. In addition, PCV2 can alter the functionality of PBMC in both healthy and PMWS pigs.
Imaging skins: an imaging modality with ultra-thin form factor (2012)

Reduction of the volume and mass of an imaging system would be advantageous in a variety of applications. However, the far-field resolution of any such system is fundamentally limited by aperture size. We present a new imaging modality based on waveguide coupling that is completely confined to a two-dimensional surface. One such imaging skin was shown to have the same resolution as a 0.5 cm diameter lens while maintaining an imaging system depth of a few micrometers.
COTS-based stick-on electricity meters for building submetering (2013)

We demonstrate a low-cost, 21 × 12 mm prototype Stick-on Electricity Meter (SEM) PCB to replace traditional in-circuit-breaker-panel current and voltage sensors for building submetering. A SEM sensor is installed on the external face of a circuit breaker to generate voltage and current signals at a 960 Hz sample rate. This allows for the computation of real and apparent power as well as capturing harmonics created by non-linear loads. The prototype sensor is built using commercially available components, resulting in a component cost of under $10 per SEM in moderate quantities. With no high-voltage install work requiring an electrician, this leads to an installed system cost that is roughly ten times lower than traditional submetering technology. Measurement results from lab characterization as well as a real-world residential dwelling installation are presented, verifying the operation of our proposed SEM sensor. The SEM sensor can resolve breaker power levels below 10W and consumes approximately 16 mA from a 5V supply.
Reduced pressure–chemical vapor deposition of Ge thick layers on Si(001) for 1.3–1.55-μm photodetection (2004)

Ge-based photodetectors operating in the low loss windows (1.3–1.6 μm) of silica fibers are highly desirable for the development of optical interconnections on silicon-on-insulator substrates. We have therefore investigated the structural and optical properties of Ge thick films grown directly onto Si(001) substrates using a production-compatible reduced pressure chemical vapor deposition system. We have first of all evidenced a Ge growth regime which is akin to a supply-limited one in the 400–750 °C temperature range (Ea=6.9 kcal mol−1). The thick Ge layers grown using a low-temperature/high-temperature approach are in a definite tensile-strain configuration, with a threading dislocation density for as-grown layers of the order of 9×108 cm−2 (annealed: &amp;lt;2×108 cm−2). The surface of those Ge thick layers is rather smooth, especially when considering the large lattice mismatch between Ge and Si. The root-mean-square roughness is indeed of the order of 0.6 nm (2 nm) only for as-grown (annealed) layers. A chemical mechanical polishing step followed by some Ge re-epitaxy can help in bringing the surface roughness of annealed layers down, however (0.5 nm). The Ge layers produced are of high optical quality. An absorption coefficient alpha equal to 4300 cm−1 (3400 cm−1) has indeed been found at room temperature and for a 1.55-μm wavelength for as-grown (annealed) layers. A 20-meV band-gap shrinkage with respect to bulk Ge (0.78 eV⇔0.80 eV) is observed as well in those tensile-strained Ge epilayers.
Homeostasis and Effector Function of Lymphopenia-Induced “Memory-Like” T Cells in Constitutively T Cell-Depleted Mice (2008)

Naive T lymphocytes acquire a phenotype similar to Ag-experienced memory T cells as a result of proliferation under lymphopenic conditions. Such "memory-like" T (T(ML)) cells constitute a large fraction of the peripheral T cell pool in patients recovering from T cell ablative therapies, HIV patients under highly active antiretroviral therapy, and in the elderly population. To generate a model that allows characterization of T(ML) cells without adoptive transfer, irradiation, or thymectomy, we developed genetically modified mice that express diphtheria toxin A under control of a loxP-flanked stop cassette (R-DTA mice). Crossing these mice to CD4Cre mice resulted in efficient ablation of CD4 single-positive thymocytes, whereas double-positive and CD8 single-positive thymocytes were only partially affected. In the periphery the pool of naive (CD44(low)CD62L(high)) T cells was depleted. However, some T cells were resistant to Cre activity, escaped deletion in the thymus, and underwent lymphopenia-induced proliferation resulting in a pool of T(ML) cells that was similar in size and turnover to the pool of CD44(high)CD62L(low) "memory phenotype" T cells in control mice. CD4Cre/R-DTA mice remained lymphopenic despite the large available immunological "space" and normal Ag-induced T cell proliferation. CD4Cre/R-DTA mice showed a biased TCR repertoire indicating oligoclonal T cell expansion. Infection with the helminth Nippostrongylus brasiliensis resulted in diminished effector cell recruitment and impaired worm expulsion, demonstrating that T(ML) cells are not sufficient to mediate an effective immune response.
Poly(<scp>L</scp>‐Lactide) microfilaments enhance peripheral nerve regeneration across extended nerve lesions (2003)

After injury, axonal regeneration occurs across short gaps in the peripheral nervous system, but regeneration across larger gaps remains a challenge. To improve regeneration across extended nerve defects, we have fabricated novel microfilaments with the capability for drug release to support cellular migration and guide axonal growth across a lesion. In this study, we examine the nerve repair parameters of non-loaded filaments. To examine the influence of packing density on nerve repair, wet-spun poly(L-Lactide) (PLLA) microfilaments were bundled at densities of 3.75, 7.5, 15, and 30% to bridge a 1.0-cm gap lesion in the rat sciatic nerve. After 10 weeks, nerve cable formation increased significantly in the filament bundled groups when compared to empty-tube controls. At lower packing densities, the number of myelinated axons was more than twice that of controls or the highest packing density. In a consecutive experiment, PLLA bundles with lower filament-packing density were examined for nerve repair across 1.4- and 1.8-cm gaps. After 10 weeks, the number of successful regenerated nerves receiving filaments was more than twice that of controls. In addition, nerve cable areas for control groups were significantly less than those observed for filament groups. Axonal growth across 1.4- and 1.8-cm gaps was more consistent for the filament groups than for controls. These initial results demonstrate that PLLA microfilaments enhance nerve repair and regeneration across large nerve defects, even in the absence of drug release. Ongoing studies are examining nerve regeneration using microfilaments designed to release neurotrophins or cyclic AMP.
Retrospective Analysis of Negative Pressure Wound Therapy in Open Foot Wounds with Significant Soft Tissue Defects (2004)

Wound cavity filling time and time to complete closure were similar in both study groups, despite the fact that patients receiving NPWT were older, had larger wounds, and had poorer nutrition. NPWT may be an effective modality for the healing of open wounds with significant defects and for reducing subsequent care.
ChemInform Abstract: A SYNTHESIS OF 3β‐HYDROXY‐5β,14α‐BUFA‐20,22‐DIENOLIDE FROM DEOXYCORTICOSTERONE (1983)

Abstract Desoxycorticosteron (Ia) wird zu (Ib) methyliert und dieses dann zu (II) hydriert, das zu (III) reduziert wird.
Ocular findings in 55 patients with Down's syndrome (1996)

Fifty-five patients with Down's syndrome were examined to evaluate the characteristics and frequency of ocular findings. Of these patients, 29 (52.7%) were hypermetropes, 7 (12.7%) were emmetropes, and 7 (12.7%) were myopes; astigmatism of more than 3.00 diopters was present in 7(12.7%) patients. Strabismus was observed in 12 (21.8%) patients. All but one of these 12 patients also had esotropia. Congenital nasolacrimal duct obstruction was present in 12 subjects (21.8%). Blepharitis was found in 19 (34.5%) cases. Nystagmus occurred in 7 (12.7%) patients. Brushfield spots were detected in only 20 (36.3%) of the patients and were more common in light-colored irides. Lens opacities were diagnosed in 11 (20%) patients, and two underwent successful cataract surgery. On fundus examination, an increased number of retinal vessels crossing the optic nerve head was detected in 21 (38.1%) cases. One of the patients had a retinal detachment. Significant but correctable ocular problems are present in patients with Down's syndrome and may interfere with the quality of life of the patient and with binocular vision. Surgical intervention may be needed for strabismus and for cataracts.
Using machine learning and expert systems to predict preterm delivery in pregnant women (2002)

Machine learning and statistical analysis were performed on 9,419 perinatal records with the goal of building a prototype expert system that would improve on the current accuracy rates achieved by manual pre-term labor and delivery risk scoring tools. Current manual scoring techniques have reported accuracy rates of 17-38%. The prototype expert system produced in this effort achieve overall accuracy rates of 53%-88% when tested on records that were not used in either statistical analysis or machine learning. Based on the success of this initial effort, the development of a full expert system to assist in pre-term delivery risk decision support, using the methods described in this paper, is planned.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
1016. Novel Chimeric Adenovirus Vaccine Vectors (2004)

The presence of circulating antibodies to adenovirus capsid proteins is a barrier to the use of adenovirus vectors for gene therapy. The prevalence of antibodies against subgroup C adenoviruses is generally high in human populations and this fact is likely to greatly limit the effectiveness of gene therapy vectors based on serotypes such as Ad5. One approach to overcoming this problem is to use adenovirus vectors that rarely cause human infections or to use adenoviruses from non-human sources, because antibodies capable of neutralizing these viruses are likely to be absent in most humans. However therapies that require multiple vector administrations will probably require a different serotype for each treatment. This will therefore necessitate the construction of adenovirus vectors based on several unrelated serotypes, which may require the construction of the appropriate complementing cell line suitable for its manufacture. In view of the above, we have investigated whether it may be possible to construct chimeric adenoviruses where most structural proteins, including the capsid, are derived from an adenovirus of one serotype, preserving the majority of the protein-protein interactions that are involved in capsid assembly, whereas the early genes encoded by the E1 and E4 regions that are responsible for transcription regulation and regulation of the host cell cycle, are retained from a different serotype that is known to result in high titer virus generation in the commonly used cell types such as HEK 293 which supplies the Ad5 E1 proteins in trans. One of the first vectors we have constructed in this way is chimeric between the chimpanzee adenoviruses Pan 5 (Simian adenovirus 22, subgroup E) and C1 (Simian adenovirus 21, subgroup B) expressing the Ebola virus (Zaire) glycoprotein (C5C1-CMVGP). In order to test the efficacy of the vector C5C1-CMVGP as a vaccine candidate, we have compared it to the Ad5 based vector (H5-CMVGP). Compared to H5-CMVGP, the C5C1-CMVGP vector yielded only a slightly decreased level of GP expression in transduced A549 cells. We evaluated GP-specific T cell and B cell responses elicited in B10BR mice vaccinated with either H5-CMVGP or C5C1-CMVGP vectors. The C5C1-CMVGP vector appeared to induce lower frequencies of gamma interferon producing CD8+ T cells with kinetics slower than the H5-CMVGP vector as determined by intracellular cytokine staining using an H-2k restricted GP-specific peptide as stimulant. The total IgG response to GP, measured by ELISA, was similar in sera from mice vaccinated with the C5C1-CMVGP or the H5-CMVGP vectors. However, the C5C1-CMVGP vector induced a more potent Th1 type response while the H5-CMVGP vector stimulated a more balanced Th1/Th2 type response. We have shown that a single administration of the H5-CMVGP vector can protect mice against a lethal challenge with a mouse-adapted strain of the Ebola Zaire virus. We are currently investigating the efficacy of the C5C1-CMVGP vector in this model.
Arrays of Ferromagnetic Iron and Cobalt Nanocluster Wires (2001)

We report a fabrication of arrays of ferromagnetic iron and cobalt nanocluster wires (NCWs), ranging from 8 to 10 nm in diameter and up to a few millimeters in length. The iron and the cobalt nanoclusters served as building blocks of the corresponding ferromagnetic NCWs. The iron and the cobalt nanoclusters were produced by thermally decomposing the corresponding metal carbonyl vapors with a resistive heater placed in the middle of a pair of permanent disk magnets. The NCWs were produced through pile-up of metallic nanoclusters along lines of magnetic flux, perpendicularly to the substrates attached to a pair of permanent disk magnet surfaces. We observed coercivities as large as 248 and 964 oersteds and remanences as high as 61 and 71% for the ferromagnetic iron and cobalt NCWs, respectively.
Gas sensors based on functionalized carbon nanotubes (2015)

Gas sensors made of carbon nanotubes without their doping and functionalization have serious shortcomings. Analysis of physics and technics of functionalization of carbon nanotubes by organic polymers, impurities, metalic nanoparticles/nanoclusters and use of nanotube in metaloxide nanocomposites for realization of detectors of different important gases with dramatically improved response, better time characteristics and smaller consumed power is carried out.
Formtion of Some Heterocycles through Ring Transformation of 1-Arylazetidin-2-ones (1977)

Treatment of 4-hydroxymethyl-1-phenylazetidin-2-one( 2 ) with methanesulfonic acid at room temperature yielded 4 -a n i l i n o -2 -o x o t e t r a h y d r o f u r a n (3).-On heating 4-benzylcarbamoyl-1-phenylazetidin-2-one (4)in methanesulfonic acid, anilinosuccinimide (6) was obtained.N-Methyl analogue (7) was also obtained from 4-methylcarbamoyl-l-phenylazetidin-2-one (5).4-(2-Piperidin0)-1-(4-methoxy-pheny1)azetidin-2-one (9) gave octahydro-4-(4-methoxy--pheny1)indolizin-2-one (10) under base-catalyzed conditions Furthermore, 3-(4-methoxyanilinomethy1)-4-hydroxy-3,4- dihydrocarbostyril (14) was ohtained by acid-catalyzed rearrangement of the corresponding 3-suhstituted l-arylazetidin-2-one ( 3 3-(4-methoxyani1ino.)-4-hydroxyocta-hydroindolizin-2-one (17) was also ohtained by the application of these nucleophilic cleavage of -N -C O -bond to the corresponding 1-arylazetidin-2-one (16).
Arachidonic acid epoxides. Demonstration through [18O]oxygen studies of an intramolecular transfer of the terminal hydroxyl group of (12S)-hydroperoxyeicosa-5,8,10,14-tetraenoic acid to form hydroxyepoxides. (1984)

Purified 12-hydroperoxyeicosa - 5, 8, 10, 14 - tetrae noic acid (12-HPETE) containing deuterium atoms at 5, 6, 8, 9, 11, 12, 14, and 15 was prepared by incubating octadeuterated arachidonic acid with a platelet preparation in air or [18O]oxygen gas. A mixture of 12-HPETE containing 16O16O:18O18O (5:4) was subsequently prepared and incubated with hematin in phosphate buffer (pH 7.4); in another experiment the same mixture of 12-HPETE was incubated with a rat lung preparation (0-30% ammonium sulfate) which lacked epoxide hydratase activity. Gas chromatography-mass spectrometry negative ion chemical ionization analysis of the extracted products after conversion into pentafluorobenzyl-trimethylsilyl derivatives indicated that the products from both incubations, i.e. 12-hydroxyeicosa - 5,8,10,14 - tetraenoic acid (12 - HETE), 8 - hydroxy - 11,12-epoxyeicosa - 5,9,14 - trienoic acid (8H-11,12-EPETE), 10H - 11,12-epoxyeicosa - 5,8,14 - trienoic acid (10H-11,12-EPETE), and 8,11,12-trihydroxyeicosa-5,9,14-trienoic acid, all retained either two [16O]oxygen or two [18O]oxygen atoms of starting 12-HPETE and that no mixed species existed which contained one [16O]oxygen and one [18O]oxygen atom. These results demonstrate for the first time in the arachidonic acid series an intramolecular transfer of the terminal hydroxyl group of the hydroperoxide of 12-HPETE to the C-8 or C-10 alkyl positions to form the hydroxyepoxides 8H-11,12-EPETE and 10H-11,12-EPETE. This reaction carried out by both hematin in the absence of protein and the rat lung preparation is suggestive of a metal-hydroperoxide-olefin "cage" complex facilitating a concerted reaction in which the terminal hydroxyl group of the hydroperoxide is trapped by alkyl free radical centers.
Dosimetry of the replication of the experiment of Madeleine Bastide (2009)

The experiment of Madeleine Bastide consists in exposing chicken eggs in incubation to the radiation of a mobile phone, and to compare the mortality of exposed eggs to that of sham eggs. The dosimetry in laboratory of this experiment comprises two parts, the measurements of the radio frequency electric field and the low frequency magnetic field at the eggs locations. The space resolution necessary to these measurements imposes the use of a dosimetric probe for the radio frequency electric field and of a specific sensor for the low frequency magnetic induction. The values of field obtained for various eggs according to their position compared to the mobile phone made it possible to test a possible correlation with their mortality.
Synthesis and characterization of binuclear zirconocene complexes linked by a bridge bis(cyclopentadienyl) ligand (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and characterization of binuclear zirconocene complexes linked by a bridge bis(cyclopentadienyl) ligandKaruna P. Reddy and Jeffrey L. PetersenCite this: Organometallics 1989, 8, 9, 2107–2113Publication Date (Print):September 1, 1989Publication History Published online1 May 2002Published inissue 1 September 1989https://pubs.acs.org/doi/10.1021/om00111a003https://doi.org/10.1021/om00111a003research-articleACS PublicationsRequest reuse permissionsArticle Views419Altmetric-Citations58LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
INDUCTION OF CELL PROLIFERATION IN THE MOUSE BLADDER BY 4‐ETHYLSULPHONYL‐NAPHTHALENE‐1 ‐SULPHONAMIDE (1969)

ABSTRACT The reaction of the bladder epithelium of mice, following stimulation with a carcinogen, 4‐ethylsulphonylnaphthalene‐1‐sulphonamide (ENS), was studied. A wave of cell division was induced in the resting epithelium, diploid and tetraploid cells being the main dividing elements; some of the high ploidy surface cells also underwent division. The cell cycle time for the diploid and tetraploid cells appeared to be identical. There was considerable variation in the intensity and timing of the onset of cell division in the bladder epithelium of individual animals. ENS caused severe damage to the surface cells of the bladder epithelium as indicated by increased lysosomes and the formation of large vacuoles.
A timing model for VLSI CMOS circuits verification and optimization (1994)

This paper presents a semi-empirical model (TIME) for timing verification of CMOS VLSI digital circuits. Its novelty lies in the introduction of a new timing parameter (latency time), which is added to the usual /spl Rfr/C effective time constant. The effects that bear in the delay of all gates are included in the TIME model: type, geometry, body effect of transistors, slope of the gate input signals, capacitance loads and threshold logic voltage (VSW). Results are shown that compare circuit delays, analytically estimated with this model, within 10% of SPICE simulations and with more accuracy than Horowitz's analytical timing model. Other results illustrate the application of TIME to W-size optimization.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>