gebhart/patrepeval huggingface dataset:
Identification and characterization of host-protective T-cell epitopes of a major surface glycoprotein (gp63) from Leishmania major. (1991)

By using a series of overlapping synthetic peptides that cover more than 75% of the amino acid sequence of the major surface glycoprotein (gp63) from Leishmania major, 11 T-cell epitopes in CBA and BALB/c mice have been identified. Six of the peptides were recognized by T cells of CBA mice recovered from L. major infection, while one was recognized by the T cells from BALB/c mice recovered from the infection following sublethal doses of gamma-irradiation. Lymph node cells from mice immunized with the peptides also responded to a number of the same peptides (seven in CBA and one in BALB/c). Peptide p10-28 induced proliferative T-cell responses in both CBA and BALB/c mice. Five of the peptides (p10-28, p22-40, p289-309, p459-471 and p467-482) induced vigorous T-cell response in CBA mice but were not recognized by T cells from recovered mice. Four other peptides (p321-336, p364-476, p372-385 and p378-396) were recognized by T cells from recovered CBA mice but could not induce a T-cell response in normal CBA mice. Three peptides (p146-171, p289-309 and p395-414) were both able to induce a T-cell response and were recognized by T cells from recovered mice. However, only two peptides (p146-171 and p467-482) were able to activate T cells, which also recognized epitopes expressed by antigen-presenting cells infected with promastigotes. T cells induced by p146-171 and p467-171 or a mixture of these two peptides were mainly CD4+ and produced interleukin (IL-2) and interferon-gamma (IFN-gamma) but not IL-4 upon antigen stimulation in vitro. These two peptides also induced a classical delayed type hypersensitivity (DTH) response in CBA mice. Furthermore, CBA mice immunized with a mixture of the two peptides in Coryne parvum or entrapped in liposomes induced significant resistance against L. major infection. The implications of these results in terms of a synthetic vaccine against leishmaniasis and the mechanism of the induction of Th1 and Th2 cells are discussed.
Restoration of Tolerance in Lupus by Targeted Inhibitory Receptor Expression (2005)

Lupus, a multigenic autoimmune condition in which a breakdown of tolerance results in the development of autoantibodies, leads to a variety of pathologic outcomes. Despite the heterogeneity of factors influencing disease susceptibility, we demonstrate that the partial restoration of inhibitory Fc receptor (FcgRIIB) levels on B cells in lupus-prone mouse strains is sufficient to restore tolerance and prevent autoimmunity. FcgRIIB regulates a common B cell checkpoint in genetically diverse lupus-prone mouse strains, and modest changes in its expression can result in either tolerance or autoimmunity. Therefore, increasing FcgammaRIIB levels on B cells may be an effective way to treat autoimmune diseases.
Cardiology's 10 greatest discoveries of the 20th century. (2002)

We present a brief summary of the 10 greatest cardiologic developments and discoveries of the 20th century Described are electrocardiography; preventive cardiology and the Framingham Study; "lipid hypotheses" and atherosclerosis; coronary care units; echocardiography; thrombolytic therapy; cardiac catheterization and coronary angiography; open-heart surgery; automatic implantable cardiac defibrillators; and coronary angioplasty. These topics are the personal choices of the authors.
On Super-Resolution With an Experimental Microwave Tomography System (2010)

The resolution of an experimental microwave tomography (MWT) system is investigated. Using two cylindrical nylon targets and an operating frequency of 5 GHz, a separation resolution of 2 mm, or 1/30 of a wavelength, is achieved. While this resolution is among the highest reported in the literature, it is not a sufficiently robust indicator of the expected resolution obtainable for complex targets, and this is shown with further examples of more complicated targets. However, the basic separation resolution limit obtained is a good way of comparing various aspects of different MWT systems.
Indoleacetic acid operon of Pseudomonas syringae subsp. savastanoi: transcription analysis and promoter identification (1990)

Expression of the indoleacetic acid (iaa) operon, which contributes to the virulence of the phytopathogenic bacterium Pseudomonas syringae subsp. savastanoi, was monitored by using broad-host-range lacZ reporter gene plasmids. A combination of translational (gene) fusions and transcriptional (operon) fusions of P. syringae subsp. savastanoi sequences to lacZ allowed localization of the iaa operon promoter. RNA recovered from P. syringae subsp. savastanoi strains was mapped with iaa operon-specific probes to precisely locate the transcription initiation site. When transcripts from an iaaM::lacZ fusion in Escherichia coli were analyzed, an identical transcription initiation site was observed. The DNA sequence of the iaa operon promoter closely resembled the consensus E. coli promoter sequence. We detected an active, constitutive level of indoleacetic acid biosynthetic gene expression during bacterial growth under a variety of conditions in the absence of host plant influence.
Recessive T cell response to poly (Glu50Tyr50) possibly caused by self tolerance. (1985)

The proliferative T cell response of inbred mouse strains to the random copolymer poly(Glu50Tyr50) (GT) was found to fall into two categories. Some strains responded only marginally (delta cpm values less than 10,000 and stimulation indices less than 3), whereas other strains mounted a substantial response (delta cpm 10,000 to 80,000, SI 3 to 30). The response is controlled by the A alpha and A beta loci of the major histocompatibility complex (MHC), as well as by genes not linked to the MHC. Because the response is selectively inhibited by monoclonal antibodies specific for the A alpha A beta molecule, we assume that its control by A loci is manifested as an A-restriction of the participating T (Ly-1high, Ly-2-) cells. It is of interest that the responsiveness is recessive in F1 hybrids of responder and nonresponder strains that are H-2-identical, but differ at their genetic background. Nonresponsiveness of these F1 mice is caused neither by a defect of antigen presentation, nor the result of immune suppression on priming or at the effector phase of the response. It is most likely the consequence of clonal deletion during the establishment of self-tolerance.
Effect of Conjugation on the Oscillator Strength of the Ruthenium(III)-Cyanamide Chromophore (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of Conjugation on the Oscillator Strength of the Ruthenium(III)-Cyanamide ChromophoreChristopher E. B. Evans, Dean Ducharme, Mark L. Naklicki, and Robert J. CrutchleyCite this: Inorg. Chem. 1995, 34, 6, 1350–1354Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/ic00110a010https://doi.org/10.1021/ic00110a010research-articleACS PublicationsRequest reuse permissionsArticle Views220Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Neospora caninum (Protozoa: apicomplexa) infections in mice. (1989)

Groups of mice were given 0 mg, 4 mg, or 2 mg of methylprednisolone acetate (MPA) 7 days prior to, the day of, and 7 days after subcutaneous inoculation with 0 or 2 x 10(5) tachyzoites of Neospora caninum. Clinical signs of disease were seen only in mice given both MPA and N. caninum tachyzoites. Mice given 4 mg MPA and N. caninum tachyzoites developed severe disseminated neosporosis and most died or were killed when comatose 11-13 days postinoculation (PI). Acute pneumonia, polymyositis, encephalitis, hepatitis, and pancreatitis were the main lesions in these mice. Mice given 2 mg MPA and N. caninum developed mild pneumonia and many mice began showing neurological signs 14 days PI. Neurological signs consisted mainly of pronounced head-tilting and associated impairment of movement. Grossly visible 1-2-mm single or multiple, white areas of discoloration were seen in the brains of many of these mice. Encephalitis, ganglioradiculoneuritis, pneumonia, and polymyositis were the main changes seen in these mice. Tissue cysts of N. caninum were only seen in mice given 2 mg MPA and were first seen 21 days PI. Tissue cysts were 16-34 by 13-29 microns and had a 1.5-3.0-microns-thick cyst wall. Tissue cysts were seen only in the brain. Mice given 4 mg MPA and tachyzoites and host cells that had been frozen for 1 wk did not develop clinical signs of infection, indicating that freezing kills tachyzoites and that viruses or other agents were not involved in the genesis of disease seen in mice given MPA and viable tachyzoites.(ABSTRACT TRUNCATED AT 250 WORDS)
Enzymatic Restriction of Mammalian Cell DNA: Evidence for Double-strand Breaks as Potentially Lethal Lesions (1985)

Permeabilized Chinese hamster cells were treated with the restriction endonucleases Pvu II and Bam H1 which generate blunt-ended and cohesive-ended DNA double-strand breaks (dsb), respectively. Cells were then assayed for their clonogenic ability. These experiments were performed to test the hypothesis that mammalian cell death following X-ray exposure arises from the induction of dsb in DNA, and via the formation of chromosomal aberrations. It was shown previously that Pvu II induces chromosome aberrations whereas Bam H1 was ineffective in this respect. The results reported here show that Pvu II simulates X-ray exposure, in causing a dose-dependent loss of the reproductive integrity of mammalian cells. Dsb generated by Pvu II, i.e. with blunt ends, can therefore be regarded as potentially clastogenic as well as potentially lethal. Bam H1 was found not to reduce cell survival in the same enzyme dose range. These results support the notion that X-irradiated mammalian cells undergo a mode of death in which dsb in the DNA cause chromosomal aberrations which are lethal as a result of loss of genetic material in the form of chromosome fragments, or as a result of chromosome bridge formation.
Nanodiamond lateral field emission vacuum logic OR gate (2011)

Reported are fabrication and characterisation of a novel vacuum logic OR gate using two identical nanodiamond lateral field emission diodes fabricated on silicon-on-insulator wafers. Each diode consists of 9000 finger-like emitters with 4 µm interelectrode spacing. High and stable emission current with low turn-on field have been observed and verified by a Fowler-Nordheim plot for each structure. Diode-resistor logic is used to realise the logic OR function. This nanodiamond vacuum logic gate is very promising for application in harsh environments.
Near‐IR Remote Release from Assemblies of Liposomes and Nanoparticles (2009)

Set free by light: Near-IR (NIR) laser-initiated remote release of fluorescent dye from complexes of liposome-gold-nanoparticle aggregates is demonstrated (see fluorescence images). Complexes of the desired size are shown to be a viable approach to the construction of vesicle-based drug-delivery systems with light-triggered remote release characteristics. This opens up a new method to manipulate liposome-based drug-delivery systems in a biocompatible way by using the near-IR spectral range.
T-cell/eosinophil interactions in the induction of asthma. (1996)

There now exists considerable support for the hypothesis that asthma represents a specialized form of cell-mediated immunity, in which cytokines and possibly other mediators secreted by activated T-lymphocytes bring about the specific accumulation and activation of eosinophils in the bronchial mucosa. This observation has very important implications for future asthma therapy, since it suggests that drugs other than glucocorticoids which inhibit T-lymphocyte function may be of therapeutic benefit, as has recently been shown in the case of cyclosporin A. Further documentation of the cytokines involved in asthma of varying clinical associations might allow a pathophysiological classification of the disease.
Liver and gut mucosa acetylation of mesalazine in healthy volunteers (2000)

As a result of the large variations in release and absorption of mesalazine in the pharmaceutical formulations and administrations, it was possible to demonstrate that acetylation occurs in the gut wall and in the liver. By comparing oral and rectal data to intravenous data, it was possible to indicate where (and to what extent) acetylation occurs in the gut wall, in the liver, or both. Rectal administration of a mesalazine suppository and intravenous administration results in hepatic acetylation. Oral administrations of mesalazine results in both gut wall and hepatic acetylation. Acetylation by the gut wall amounts to 30% of the dose for gastroresistant tablets and to 40% of the dose for prolonged-release tablets.
평생학습도시 조성을 위한 추진모형 연구 (2004)

“평생학습도시”는 시ㆍ군ㆍ구 등 기초 지자체 단위의 주민 평생학습지원시스템으로서, 정부가 2001년부터 조성 정책을 추진하고 있다. 평생학습도시는 지역사회내의 다양한 교육기관과 주민의 학습활동에 이용할 수 있는 다양한 인적 물적 자원을 활용하여 학습활동을 지원함으로서 개인과 지역사회의 문화적 경제적 정치적 능력을 향상시켜 지역사회의 발전을 도모하는...
Hausmannite Mn<sub>3</sub>O<sub>4</sub>nanorods: synthesis, characterization and magnetic properties (2006)

High yield hausmannite (Mn3O4) nanorods with diameters of about 100 nm and lengths up to 15–20 µm have been prepared by vacuum calcining of the precursor (Mn3O4+MnOOH), which was hydrothermally prepared by the reaction of PEG-20000 and KMnO4 at 180 °C for 20 h. Transmission electron microscopy and the selected-area electron diffraction pattern reveal that these Mn3O4 nanorods show single-crystal growth along the [100] direction. The PEG-20000 and the calcination conditions have key effects on the morphology and phase purity of the product. Magnetism measurements show that the blocking temperature for these Mn3O4 nanorods is 41 K, which agrees with the bulk material value, whereas the remnant magnetization and coercivity are 0.89 μB and 6177 Oe respectively.
Pentafluorophenylimido Alkylidene Complexes of Molybdenum and Tungsten (2012)

Pentafluorophenylimido alkylidene complexes of molybdenum and tungsten have been prepared in good yields. Examples include Mo(NC6F5)(CHCMe2Ph)(Me2Pyr)2 (Me2Pyr = 2,5-dimethylpyrrolide), W(NC6F5)(CH-t-Bu)(DME)(Pyrrolide)2, Mo(NC6F5)(CHCMe2Ph)[OC(CF3)3]2, W(NC6F5)(CH-t-Bu)(DME)[OC(CF3)3]2, Mo(NC6F5)(CHCMe2Ph)[OC(C6F5)3]2, W(NC6F5)(CH-t-Bu)[OC(C6F5)3]2, Mo(NC6F5)(CHCMe2Ph)(ODFT)2 (ODFT = O-2,6-(C6F5)2C6H3), and W(NC6F5)(CH-t-Bu)(ODFT)2. Treatment of W(NC6F5)(CH-t-Bu)(DME)[OC(CF3)3]2 with ethylene led to formation of W(NC6F5)(CH2CH2CH2)[OC(CF3)3]2, which has a TBP structure in which the metallacyclobutane ring lies in the equatorial plane. 2,3-Dicarbomethoxynorbornadiene is polymerized by M(NC6F5)(CHR′)(ODFT)2 initiators to give cis,isotactic-poly(DCMNBD).
Studies on biliary excretion in the rabbit - II. The relationship between the chemical structure of certain natural or synthetic pentacyclic triterpenes and their icterogenic activity - Part 2: The substituents on carbon atoms 17, 19, 20 and 22 (1964)

Abstract Previously reported work in this series (Brown, Rimington &amp; Sawyer 1963) produced evidence that icterogenic activity (i.a.) of the pentacyclic triterpene acids is based upon the presence of a β-equatorially orientated hydroxyl group at C (3), or a hydroxyl at C (24), and a 22β-angeloyloxy side chain in the molecule of the particular active compound. The second part of this work, dealing with certain structural variations in triterpenes of the oleanane, 24-noroleanane (hedragane) and ursane series, is reported in this paper. These variations involve i.a. esterification of the C (28) carboxyl, saturation of the 22β-angeloyl side chain or replacement of this by simple saturated aliphatic acids. Assays are recorded of a further thirteen of these compounds for icterogenic activity using the modified technique described in the second paper of this series (Brown et al. 1963). It has been concluded from this work that the icterogenicity of 3β- or 24β-hydroxy triterpene acids is undoubtedly associated with the 22β-angeloyloxy side chain. Removal of the esterifying group at C (22) or its replacement by a simple saturated fatty acid residue is followed by complete loss of activity. Esterification of the C (28) carboxyl produces a considerable decrease in icterogenic potency due, probably, to a decrease in solubility of the compound.
Determination of L-Methionine with L-Methionine γ-Lyase (1981)

Abstract The simple and sensitive spectrophotometric method for the determination of L-methionine with L-methionine γ-lyase (EC 4.4.1. 11) has been presented. The method consists of the α, γ-elimination reaction of L-methionine with L-methionine γ-lyase and the spectrophotometric determination of one of the reaction products: α-ketobutyrate, methanethiol or ammonia.
Heat Shock Protein 27 Overexpression Mitigates Cytokine-Induced Islet Apoptosis and Streptozotocin-Induced Diabetes (2009)

Beta-cell apoptosis occurs in diabetes mellitus (DM). Heat shock protein (HSP) 27 (human homolog of rodent HSP25) mitigates stress-induced apoptosis but has not been studied in beta-cells. We tested whether HSP27 overexpression attenuates streptozotocin (SZ)-induced DM in vivo and cytokine-induced islet apoptosis in vitro. DM was ascertained by ip glucose tolerance testing, and fasting serum insulin/glucose was measured. Pancreas was stained for insulin, HSP27, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, and insulin content was measured. HSP25/27 was measured by immunoblotting, isoelectric focusing, and RT-PCR. Islet HSP25/27 oligomerization and inhibitory kappaB protein kinase gamma (nuclear factor kappaB essential modulator) binding were assessed by coimmunoprecipitation. HSP27 transgene (TG) in pancreas localized predominantly in beta-cells. Baseline pancreatic insulin levels in wild-type (WT) and HSP27TG mice were similar, but lower in WT than HSP27TG after SZ (P < 0.01). Intraperitoneal glucose tolerance testing confirmed protection from SZ-DM in HSP27TG. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling and inducible nitric oxide synthase staining were increased in WT vs. HSP27TG islets (P < 0.05) after SZ. Caspase-3 activity was lower in islets from HSP27TG vs. WT mice after cytokine stress in vitro (P < 0.05). There was more HSP25 plus 27 protein from HSP27TG islets than HSP25 from WT (P < 0.01). HSP25 protein but not mRNA was increased in HSP27TG mice. Isoelectric focusing showed similar relative HSP phosphorylation in HSP27TG and WT (P > 0.05). HSP27 bound native HSP25 in TG islets; both bound to inhibitory kappaB protein kinase gamma (nuclear factor kappaB essential modulator). These data show islet protection by HSP27 by mitigation of apoptosis, possibly through nuclear factor kappaB regulation.
Oncoretrovirus and Lentivirus Vectors Pseudotyped with Lymphocytic Choriomeningitis Virus Glycoprotein: Generation, Concentration, and Broad Host Range (2002)

Lymphocytic choriomeningitis virus (LCMV) is a noncytopathic arenavirus shown to infect a broad range of different cell types. Here, we combined the beneficial characteristics of the LCMV glycoprotein (LCMV-GP) and those of retroviral vectors to generate a new, safe, and efficient gene transfer system. These LCMV-GP pseudotypes were systematically compared with vectors containing the widely used amphotropic murine leukemia virus envelope (A-MLVenv) or the vesicular stomatitis virus G protein (VSV-G). Production of LCMV-GP-pseudotyped oncoretroviral and lentiviral vectors by transient transfection resulted in vector titers similar to those with A-MLVenv or VSV-G. In contrast to A-MLVenv particles, LCMV-GP pseudotypes could be efficiently concentrated by ultracentrifugation without loss of vector titer. Unlike the cell-toxic VSV-G, a stable retroviral packaging cell line constitutively expressing LCMV-GP could be established. Vectors pseudotyped with LCMV-GP efficiently transduced many cell lines from different species and tissues relevant for gene therapy. Transduction of human glioma cells was studied in detail. These cells are a major target for cancer gene therapy and were transduced more efficiently with LCMV-GP-pseudotyped vectors than with the generally used A-MLVenv particles. The high stability, low toxicity, and broad host range make LCMV-GP-pseudotyped vectors attractive for gene transfer applications. The recombinant LCMV-GP-pseudotyped vectors will also allow functional characterization of naturally occurring and recombinant LCMV-GP variants.
De kern van de zaak: evaluatie van nucleair non-proliferatie verdrag (2005)

status: Published
Adversarial Machine Learning at Scale (2016)

Adversarial examples are malicious inputs designed to fool machine learning models. They often transfer from one model to another, allowing attackers to mount black box attacks without knowledge of the target model's parameters. Adversarial training is the process of explicitly training a model on adversarial examples, in order to make it more robust to attack or to reduce its test error on clean inputs. So far, adversarial training has primarily been applied to small problems. In this research, we apply adversarial training to ImageNet. Our contributions include: (1) recommendations for how to succesfully scale adversarial training to large models and datasets, (2) the observation that adversarial training confers robustness to single-step attack methods, (3) the finding that multi-step attack methods are somewhat less transferable than single-step attack methods, so single-step attacks are the best for mounting black-box attacks, and (4) resolution of a "label leaking" effect that causes adversarially trained models to perform better on adversarial examples than on clean examples, because the adversarial example construction process uses the true label and the model can learn to exploit regularities in the construction process.
A Robust Reputation System for P2P and Mobile Ad-hoc Networks (2004)

Reputation systems can be tricked by the spread of false reputation ratings, be it false accusations or false praise. Simple solutions such as exclusively relying on one&amp;apos;s own direct observations have drawbacks, as they do not make use of all the information available. We propose a fully distributed reputation system that can cope with false disseminated information. In our approach, everyone maintains a reputation rating and a trust rating about everyone else that they care about. From time to time first-hand reputation information is exchanged with others; using a modified Bayesian approach we designed and present in this paper, only second-hand reputation information that is not incompatible with the current reputation rating is accepted. Thus, reputation ratings are slightly modified by accepted information. Trust ratings are updated based on the compatibility of second-hand reputation information with prior reputation ratings. Data is entirely distributed: someone&amp;apos;s reputation and trust is the collection of ratings maintained by others. We enable redemption and prevent the sudden exploitation of good reputation built over time by introducing re-evaluation and reputation fading.