gebhart/patrepeval huggingface dataset:
Morphology and Spatial Distribution of ZnO Formed in Discharged Alkaline Zn/MnO[sub 2] AA Cells (2003)

A novel experimental technique was developed to extract the KOH solution from the porous zinc electrode in an inert environment. This technique permits the ZnO/electrolyte interface, the ZnO/Zn particle interface, and the spatial distribution of ZnO in the porous anode in commercial alkaline AA batteries to be characterized for the first time by optical and scanning electron microscopy. Both type I and type II ZnO, previously observed in oxidation of planar zinc electrodes, were found in each completely discharged zinc particle in the porous electrode. The morphology, size, and location of these two ZnO forms strongly suggested that they were produced by a solution-precipitation route. The drain rate had a significant impact on the spatial distribution of ZnO in the anode. The segregation of ZnO near the separator became dominant at drain rates higher than 500 mA. The mechanisms by which the morphology and segregation of ZnO within the porous electrode could result in the reduced battery runtime are discussed. © 2003 The Electrochemical Society. All rights reserved.
Translating Clinical Research into Clinical Practice: Impact of Using Prediction Rules To Make Decisions (2006)

Clinical prediction rules, sometimes called clinical decision rules, have proliferated in recent years. However, very few have undergone formal impact analysis, the standard of evidence to assess their impact on patient care. Without impact analysis, clinicians cannot know whether using a prediction rule will be beneficial or harmful. This paper reviews standards of evidence for developing and evaluating prediction rules; important differences between prediction rules and decision rules; how to assess the potential clinical impact of a prediction rule before translating it into a decision rule; methodologic issues critical to successful impact analysis, including defining outcome measures and estimating sample size; the importance of close collaboration between clinical investigators and practicing clinicians before, during, and after impact analysis; and the need to measure both efficacy and effectiveness when analyzing a decision rule's clinical impact. These considerations should inform future development, evaluation, and use of all clinical prediction or decision rules.
Measurement of a rugulosin-producing endophyte in white spruce seedlings (2005)

Previous studies conducted in growth chambers had demonstrated the successful experimental inoculation of white spruce seedlings with anti-insectan toxin producing needle endophytes. Needles colonized with the rugulosin-producing endophyte 5WS22E1 (DAOM 229536) contained rugulosin in concentrations that impaired spruce budworm growth. Here we report experimental inoculations conducted under nursery conditions. To improve the reliability of detecting successful colonization, a polyclonal assay was developed for the target endophyte 5WS22E1. It was able to reliably detect the fungus in 500 ng subsamples of colonized needles. Seventeen months post-inoculation, 330 seedlings from 1235 inoculated were colonized. A random selection of 113 colonized seedlings was analyzed for rugulosin. Needles of most (90%) contained detectable concentrations of rugulosin. The range and distribution of the rugulosin concentrations was similar to that found in earlier tests done in growth chambers.
Specific Roles of Each TCR Hemichain in Generating Functional Chain-Centric TCR (2015)

TCRα- and β-chains cooperatively recognize peptide-MHC complexes. It has been shown that a "chain-centric" TCR hemichain can, by itself, dictate MHC-restricted Ag specificity without requiring major contributions from the paired TCR counterchain. Little is known, however, regarding the relative contributions and roles of chain-centric and its counter, non-chain-centric, hemichains in determining T cell avidity. We comprehensively analyzed a thymically unselected T cell repertoire generated by transducing the α-chain-centric HLA-A*02:01(A2)/MART127-35 TCRα, clone SIG35α, into A2-matched and unmatched postthymic T cells. Regardless of their HLA-A2 positivity, a substantial subset of peripheral T cells transduced with SIG35α gained reactivity for A2/MART127-35. Although the generated A2/MART127-35-specific T cells used various TRBV genes, TRBV27 predominated with >10(2) highly diverse and unique clonotypic CDR3β sequences. T cells individually reconstituted with various A2/MART127-35 TRBV27 TCRβ genes along with SIG35α possessed a wide range (>2 log orders) of avidity. Approximately half possessed avidity higher than T cells expressing clone DMF5, a naturally occurring A2/MART127-35 TCR with one of the highest affinities. Importantly, similar findings were recapitulated with other self-Ags. Our results indicate that, although a chain-centric TCR hemichain determines Ag specificity, the paired counterchain can regulate avidity over a broad range (>2 log orders) without compromising Ag specificity. TCR chain centricity can be exploited to generate a thymically unselected Ag-specific T cell repertoire, which can be used to isolate high-avidity antitumor T cells and their uniquely encoded TCRs rarely found in the periphery because of tolerance.
Draize Rabbit Eye Test Compatibility with Eye Irritation Thresholds in Humans: A Quantitative Structure-Activity Relationship Analysis (2003)

Draize rabbit eye test scores, as modified maximum average score (MMAS), for 68 pure bulk liquids were adjusted by the liquid-saturated vapor pressure P. These 68 adjusted scores, as log (MMAS/P), were shown to be completely equivalent to eye irritation thresholds (EIT), expressed as log (1/EIT), for 23 compounds in humans. Thus, for the first time the Draize eye test in rabbits for pure bulk liquids is shown to be perfectly compatible with eye irritation thresholds in humans. The total data set for 91 compounds was analyzed by the general solvation equation of Abraham. Values of log (MMAS/P) or log (1/EIT) could be fitted to a five-parameter equation with R2 = 0.936, SD = 0.433, AD = 0.000, and AAD = 0.340 over a range of 9.6 log units. When divided into a training set of 45 compounds, the corresponding equation could be used to predict the remaining 46 compounds in a test set with AD = -0.037 and AAD = 0.345 log units. Thus, the 91-compound equation can now be used to predict further EIT values to around 0.4 log units. It is suggested that the mechanism of action in the Draize test and in the human EIT involves passive transfer of the compound to a biophase that is quite polar, is a strong hydrogen bond base, a moderate hydrogen bond acid, and quite hydrophobic. The biophase does not resemble water or plasma, but resembles an organic solvent such as N-methylformamide.
Problems, Obstacles, and Complications of Limb Lengthening by the Ilizarov Technique (1990)

Difficulties that occur during limb lengthening were subclassified into problems, obstacles, and complications. Problems represented difficulties that required no operative intervention to resolve, while obstacles represented difficulties that required an operative intervention. All intraoperative injuries were considered true complications, and all problems during limb lengthening that were not resolved before the end of treatment were considered true complications. The difficulties that occurred during limb lengthening include muscle contractures, joint luxation, axial deviation, neurologic injury, vascular injury, premature consolidation, delayed consolidation, nonunion, pin site problems, and hardware failure. Late complications are those of loss of length, late bowing, and refracture. Joint stiffness may also be a permanent residual complication. Pain and difficulty sleeping are other problems that arise during limb lengthening, especially in the more extensive cases. Forty-six patients had 60 limb segments lengthened between 1.0 and 16.0 cm, with a mean of 5.6 cm. The average treatment time was approximately one month per centimeter for single-level lengthenings with no deformity and 1.2 months per centimeter with deformity correction. The lengthening index for double-level lengthening was 0.57 month per centimeter with no deformity and 0.90 month per centimeter with correction of deformity. In adults, the lengthening index was 1.7 months per centimeter for single-level and 1.1 months per centimeter for double-level lengthening. There were 35 problems that had to be resolved in the outpatient clinic. There were 11 obstacles that required additional operative intervention to resolve. There were 27 true complications, of which 17 were considered minor and ten were considered major complications. Of the major complications, three interfered with achieving the original goals of treatment. All three required further operative intervention to achieve the original goal. These were nonunion in one and late bowing in two. Despite these problems, obstacles, and complications, the original goals of surgery were achieved in 57 of the 60 limb segments treated. Patient satisfaction was achieved in 94% of 46 cases.
Mrr instigates the SOS response after high pressure stress in <i>Escherichia coli</i> (2005)

The bacterial SOS response is not only a vital reply to DNA damage but also constitutes an essential mechanism for the generation of genetic variability that in turn fuels adaptation and resistance development in bacterial populations. Despite the extensive depiction of the SOS regulon itself, its activation by stresses different from typical DNA damaging treatments remains poorly characterized. Recently, we reported the RecA- and LexA-dependent induction of the SOS response in Escherichia coli MG1655 after exposure to high hydrostatic pressure (HP, approximately 100 MPa), a physical stress of which the cellular effects are not well known. We now found this HP mediated SOS response to depend on RecB and not on RecF, which is a strong indication for the involvement of double strand breaks. As the pressures used in this work are thermodynamically unable to break covalent bonds in DNA, we hypothesized the involvement of a cellular function or pathway in the formation of this lesion. A specialized screening allowed us to identify the cryptic type IV restriction endonuclease Mrr as the final effector of this pathway. The HP SOS response and its corresponding phenotypes could be entirely attributed to the HP triggered activation of Mrr restriction activity. Several spontaneously occurring alleles of mrr, incapable of triggering the HP-induced SOS response, were isolated and characterized. These results provide evidence for a specific pathway that transmits the perception of HP stress to induction of the SOS response and support a role for Mrr in bacterial stress physiology.
Synthesis, Structure−Activity Relationships, and Pharmacokinetic Profiles of Nonpeptidic Difluoromethylene Ketones as Novel Inhibitors of Human Chymase (2001)

Potent human chymase inhibitors with high enzymatic selectivity and satisfactory metabolic stability were obtained by replacing the Val-Pro (P3-P2) dipeptide portion of the previously described inhibitor 1 with a nonpeptidic pyrimidinone skeleton. The potency of the novel compounds was further enhanced by the introduction of carbamoyl-substituted difluoromethylene ketone moieties. The most potent chymase inhibitor of the newly created series was 2u (Y-40018), which had a K(i) of 2.62 nM. Compound 2u possessed high selectivity for human chymase since it lacked significant activity toward other representative human proteolytic enzymes. Moreover its strict specificity for human chymase suggested that 2u strongly inhibited human and canine chymases but not rat and mouse ones. Pharmacokinetic studies in rats and dogs indicated that 2u was absorbed rapidly after oral administration and had satisfactory bioavailability in these experimental animal species (rat, 17%; dog, 32%). In conclusion, 2u is a novel, potent, and orally active chymase inhibitor which would prove very useful in revealing the precise roles of the latter in various pathophysiological processes.
Engagement and Nonusage Attrition With a Free Physical Activity Promotion Program: The Case of 10,000 Steps Australia (2015)

Compared to other freely accessible Web-based health behavior interventions, the 10,000 Steps program showed high engagement. The use of an app alone or in addition to the website can enhance program engagement and reduce risk of attrition. Better understanding of participant reasons for reducing engagement can assist in clarifying how to best address this issue to maximize behavior change.
Full vector measurements of converging terahertz beams with linear, circular, and cylindrical vortex polarization (2014)

The complete vector information of converging terahertz (THz) beams with linear, circular, and cylindrical vortex polarization are precisely measured by using a THz digital holographic imaging system. The transverse (Ex, Ey) and longitudinal (Ez) polarization components of the THz fields around the focal point are separately obtained utilizing the detection crystals with different crystalline orientations. The measured results are in good agreement with the theoretical expectations. This imaging technique provides an effective way for revealing the vector diffraction properties of the THz electro-magnetic waves.
Exploring One Accord for the Business of Aged Care Industry: The CEO's Perspective (2015)

Three million Australians are aged over 65 years with a third requiring some level of assistance with their everyday activities Australian Government expenditure will need to increase from 0.8% of GDP in 2010 to 1.8% of GDP by 2050 as ABS projected growth of 65 years or over to increases from 15 per cent to 21 per cent by 2026 and 28 per cent by 2056 (AIHW 2012, ABS 2011). With the aged care industry growing faster than any other industry research, benefit realization in leadership, efficiency and productivity will impact to a great extent across the industry. Using a convenience sample, the purpose of this paper was to develop a better understanding of trends, issues, challenges and opinions of 24 Chief Executive Officers (CEO) and sector leaders in aged care toward government reforms and current aged care policy in Australia, in 2013. The in-depth interviews advanced the topics of aged care work force, work conditions, attractiveness of the industry to investment, funding models, government relationships, research data management and the future. The paper culminates in a research and practice agenda for the future and accentuates the areas that our sector leaders believe need focus.
The Reactivity of Molecules Trapped within the SAPO-34 Cavities in the Methanol-to-Hydrocarbons Reaction (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTThe Reactivity of Molecules Trapped within the SAPO-34 Cavities in the Methanol-to-Hydrocarbons ReactionBjørnar Arstad and Stein KolboeView Author Information Department of Chemistry, University of Oslo P.O. Box 1033, N-0315 Oslo, Norway Cite this: J. Am. Chem. Soc. 2001, 123, 33, 8137–8138Publication Date (Web):July 26, 2001Publication History Received13 March 2001Published online26 July 2001Published inissue 1 August 2001https://pubs.acs.org/doi/10.1021/ja010668thttps://doi.org/10.1021/ja010668trapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views2397Altmetric-Citations309LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alcohols,Alkyls,Catalysts,Hydrocarbons,Molecules Get e-Alerts
Visualization of the timing of gene amplification during multistep head and neck tumorigenesis. (2000)

Head and neck tumorigenesis is thought to represent a multistep process whereby carcinogen exposure leads to genetic instability in the tissue and the accumulation of specific genetic events, which result in dysregulation of proliferation, differentiation, and cell loss and the acquisition of invasive capacity. Chromosome 11q13 amplification is frequently observed in head and neck squamous cell carcinoma (HNSCC), and the amplified gene products are assumed to play important functional roles in the tumor phenotype. However, it is not well understood whether gene amplification precedes carcinoma development or results from the unstable nature of intact tumors. To determine the timing of gene amplification during tumorigenesis, tissue sections from amplified HNSCC specimens (containing a contiguous transition from normal epithelium to hyperplasia to dysplasia to carcinoma) were probed for INT2 gene copy number by chromosome in situ hybridization. In addition, representative epithelia were microdissected from the tissue sections, and the DNA was isolated and assessed for INT2 gene copy number by semiquantitative PCR. In those cases containing amplified INT2 in the carcinoma, gene amplification appeared to precede HNSCC development. In one case, INT2 gene amplification appeared in the hyperplasia to dysplasia transition, whereas in two other cases, gene amplification was apparent at dysplasia. These results suggest that gene amplification can occur early during head and neck tumorigenesis and that genetic instability is an important driving force in the tumorigenesis process.
Combining Visual Rehabilitative Training and Noninvasive Brain Stimulation to Enhance Visual Function in Patients With Hemianopia: A Comparative Case Study (2011)

Results of preliminary case comparisons suggest that occipital cortical tDCS may enhance recovery of visual function associated with concurrent VRT through visual cortical reorganization. Future studies may benefit from incorporating protocol refinements such as those described here, which include global capture of function, control for potential confounds, and investigation of underlying neural substrates of recovery.
Nucleic Acid Nanotechnology (2004)

Nucleic acids are increasingly used to build nanometer-scale structures that may be used in future nanotechnology devices. In his Perspective,
 Yan
 identifies two key goals in this area: to perform controlled mechanical movements, and to produce complex structures from simple molecular building blocks. He highlights the reports of
 Liao and Seeman, whose ribosome-like DNA devices mimic nature9s biological motors to create nanometer-scale machines, and of
 Chworos
 et al., who create nanometer-scale materials by organizing RNA components into precisely controlled ensembles. Yan identifies several areas, such as error correction and scaled-up self-assembly, that require particular attention if the potential of nucleic acid-based nanotechnology is to be fulfilled.
Cross-Linking of Collagen (1973)

The formation of collagen cross-links is attributable to the presence of two aldehyde-containing amino acids which react with other amino acids in collagen to generate difunctional, trifunctional, and tetrafunctional cross-links. A necessary prerequisite for the development of these cross-links is that the collagen molecules be assembled in the naturally occurring fibrous polymer. Once this condition is met, cross-linking occurs in a spontaneous, progressive fashion. The chemical structures of the cross-links dictate that very precise intermolecular alignments must occur in the collagen polymer. This seems to be a function of each specific collagen because the relative abundance of the different cross-links varies markedly, depending upon the tissue of origin of the collagen.
Two aspects of the excitatory influence of histamine on hippocampal neurons in guinea pigs. (1991)

The effect of histamine (Hi) on CA3 pyramidal cells in guinea pig hippocampal slices was studied by means of intracellular recordings in association with electrical stimulation to the mossy fibers of granular cells. The amplitude of EPSPs and spikes was augmented significantly by Hi at concentrations of 10(-7) to 10(-6) M. The stimulatory effect of Hi was mimicked by the H2-agonist dimaprit (10(-6) M), but not by the H1-agonist 2-thiazolylethylamine (10(-5) M). An increase in EPSPs or spikes was provoked during perfusion of dibutyryl cAMP at concentrations higher than 10(-6) M. Intracellular application of cAMP to the pyramidal cells augmented the amplitude of EPSPs. The amplitude of IPSPs provoked by GABA was reduced by simultaneous perfusion of Hi at concentrations of 10(-7) M to 10(-6) M. The Hi-induced reduction was not blocked by either pyrilamine, cimetidine or thioperamide. It became clear that Hi exerts an excitatory influence on the hippocampal neurons, not only by stimulating the excitatory system via an H2-receptor coupled with Hi-sensitive adenylate cyclase, but also by depressing the inhibitory system, which may be mediated by GABA.
Synthesis of the potent influenza neuraminidase inhibitor 4-guanidino Neu5Ac2en. X-Ray molecular structure of 5-acetamido-4-amino-2,6-anhydro-3,4,5-trideoxy-D-erythro-L-gluco-nononic acid (1995)

An efficient and high-yielding synthesis of 4-guanidino Neu5Ac2en, the potent anti-influenza A and B compound, is described. The route exploits a stereospecific introduction of the key nitrogen functionality at C-4 via an oxazoline intermediate. Three different methods for the final-step conversion of the 4-amino into 4-guanidino derivatives are described. To explore the structure–activity relationship in this region of the molecule, a series of substituted guanidino derivatives were synthesized and their activity is described.
THE REFERENCE HUMAN GENOME DEMONSTRATES HIGH RISK OF TYPE 1 DIABETES AND OTHER DISORDERS (2010)

Personal genome resequencing has provided promising lead to personalized medicine. However, due to the limited samples and the lack of case/control design, current interpretation of personal genome sequences has been mainly focused on the identification and functional annotation of the DNA variants that are different from the reference genome. The reference genome was deduced from a collection of DNAs from anonymous individuals, some of whom might be carriers of disease risk alleles. We queried the reference genome against a large high-quality disease-SNP association database and found 3,556 disease-susceptible variants, including 15 rare variants. We assessed the likelihood ratio for risk for the reference genome on 104 diseases and found high risk for type 1 diabetes (T1D) and hypertension. We further demonstrated that the risk of T1D was significantly higher in the reference genome than those in a healthy patient with a whole human genome sequence. We found that the high T1D risk was mainly driven by a R260W mutation in PTPN22 in the reference genome. Therefore, we recommend that the disease-susceptible variants in the reference genome should be taken into consideration and future genome sequences should be interpreted with curated and predicted disease-susceptible loci to assess personal disease risk.