gebhart/patrepeval huggingface dataset:
Minimizing the Impact of Scan Compression (2007)

Scan is widely accepted as the basis for reducing test cost and improving quality, however its effectiveness is compromised by increasingly complex designs and fault models that can result in high scan data volume and application time. The authors present a scan compression method designed for minimal impact in all aspects: area overhead, timing, and design flow. Easily adopted on top of existing scan designs, the method is fully integrated in the scan synthesis and test generation flows. Data and test time compressions of over 10times were obtained on industrial designs with negligible overhead and no impact on schedule.
Involvement of the Kynurenine Pathway in Human Glioma Pathophysiology (2014)

The kynurenine pathway (KP) is the principal route of L-tryptophan (TRP) catabolism leading to the production of kynurenine (KYN), the neuroprotectants, kynurenic acid (KYNA) and picolinic acid (PIC), the excitotoxin, quinolinic acid (QUIN) and the essential pyridine nucleotide, nicotinamide adenine dinucleotide (NAD(+)). The enzymes indoleamine 2,3-dioxygenase-1 (IDO-1), indoleamine 2,3-dioxygenase-2 (IDO-2) and tryptophan 2,3-dioxygenase (TDO-2) initiate the first step of the KP. IDO-1 and TDO-2 induction in tumors are crucial mechanisms implicated to play pivotal roles in suppressing anti-tumor immunity. Here, we report the first comprehensive characterisation of the KP in 1) cultured human glioma cells and 2) plasma from patients with glioblastoma (GBM). Our data revealed that interferon-gamma (IFN-γ) stimulation significantly potentiated the expression of the KP enzymes, IDO-1 IDO-2, kynureninase (KYNU), kynurenine hydroxylase (KMO) and significantly down-regulated 2-amino-3-carboxymuconate semialdehyde decarboxylase (ACMSD) and kynurenine aminotransferase-I (KAT-I) expression in cultured human glioma cells. This significantly increased KP activity but significantly lowered the KYNA/KYN neuroprotective ratio in human cultured glioma cells. KP activation (KYN/TRP) was significantly higher, whereas the concentrations of the neuroreactive KP metabolites TRP, KYNA, QUIN and PIC and the KYNA/KYN ratio were significantly lower in GBM patient plasma (n = 18) compared to controls. These results provide further evidence for the involvement of the KP in glioma pathophysiology and highlight a potential role of KP products as novel and highly attractive therapeutic targets to evaluate for the treatment of brain tumors, aimed at restoring anti-tumor immunity and reducing the capacity for malignant cells to produce NAD(+), which is necessary for energy production and DNA repair.
Arylboronic acids. Medium-size ring-containing boronic ester groups (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTArylboronic acids. Medium-size ring-containing boronic ester groupsWilliam M. Cummings, Clifford H. Cox, and H. R. SnyderCite this: J. Org. Chem. 1969, 34, 6, 1669–1674Publication Date (Print):June 1, 1969Publication History Published online1 May 2002Published inissue 1 June 1969https://pubs.acs.org/doi/10.1021/jo01258a029https://doi.org/10.1021/jo01258a029research-articleACS PublicationsRequest reuse permissionsArticle Views360Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Expression and immunogenicity of an 18-residue epitope of HIV1 gp41 inserted in the flagellar protein of a Salmonella live vaccine (1995)

A synthetic oligonucleotide specifying residues 735-752 of the product of the env gene of HIV1 IIIB was inserted by blunt-end ligation at restriction sites in the hypervariable, antigenically determinant region IV of two flagellin genes. Its integration, in frame and correct orientation, into gene fliC(d) in plasmid pLS408 allowed production of functional flagella when the plasmid was placed in a flagellin-negative aroA live-vaccine Salmonella dublin strain, SL5928. Bacteria thus made motile were immobilized and agglutinated by anti-(735-752 peptide) serum; expression was also shown by immunoelectron-microscopy and by Western blot of whole-cell lysates. Enzyme immunoassay (EIA) of sera of mice given three doses by intraperitoneal injection of the live-vaccine strain producing chimeric flagellin, or of concentrated flagella from it, showed production of antibody with affinity for the peptide, and in some sera, also for r-gp160. Pooled serum from mice given five i.p. doses of the live vaccine strain expressing the gp41 epitope at the surface of its flagellar filaments had higher titres of anti-peptide and anti-r-gp160 antibody and weak neutralizing activity on the IIIB isolate (90% neutralization at 1/100). The sera of nine mice given two doses of the live vaccine by the oral route had either no or only very low titres of antibody to flagellar antigen d; they were therefore not tested for anti-peptide activity.
Cultivation of human melanomas in soft agar. Factors influencing plating efficiency and chemosensitivity (1981)

As part of a programme to study the predictive clinical value of a soft agar assay for measuring chemosensitivity of human melanomas in vitro, we have observed the effect of three disaggregation methods on the yield of tumor cells, plating efficiency in soft agar and chemosensitivity. The yields and plating efficiencies obtained, as well as sensitivity to DTIC, CCNU, vinblastine and abrin, were about the same whether collagenase/pronase/DNase-treatment, trypsin/EDTA-treatment or mechanical treatment was used. When melanoma xenografts of different sizes were studied, an inverse relationship between tumor size and plating efficiency was found, whereas chemosensitivity was unaffected by tumor size. The highest plating efficiencies of melanoma cells, both from patient biopsies and from xenografts, were obtained when red blood cells were present and a low oxygen concentration (5%) was used. The results demonstrate that, in the case of melanomas, the fraction of tumor cells that are clonogenic in vitro depends on the size of the tumors, and even more so on the culture conditions used. An important finding was that chemosensitivity in vitro appears to be unaffected by the disaggregation method and by tumor size.
Surface activated bonding for new flip chip and bumpless interconnect systems (2003)

As microelectronic integration trends move toward ultrafine density flip chips and bumpless interconnect systems, it is necessary to develop a low cost, ultra-high density, low-damage and low-temperature interconnect technology. To satisfy this requirement, a new bonding method, the surface activated bonding (SAB), was introduced. In this process, the bonding pads on chips and bumps on substrates are first cleaned by Ar plasma or atom beam irradiation, precisely aligned with an accuracy of less than /spl plusmn/0.5 /spl mu/m. Then they are bonded directly to each other only by contact at room temperature or low temperature in a newly developed flip chip bonder. This procedure, which enables the bonding in an inert ambient atmosphere of N/sub 2,/ under atmospheric pressure, cuts down the bonding condition requirements and decreased the process cost. Some preliminary experiments have led to Au-Au and Au-Cu direct bonding, demonstrating the performance of the new bonding system.
<i>Escherichia coli</i> Sequence Type ST131 as an Emerging Fluoroquinolone-Resistant Uropathogen among Renal Transplant Recipients (2009)

Among 40 Escherichia coli urine isolates from renal transplant recipients (Galveston, TX, 2003 to 2005), sequence type ST131 (O25:H4) was highly prevalent (representing 35% of isolates overall and 60% of fluoroquinolone-resistant isolates), virulent appearing, antimicrobial resistant (but extended-spectrum-cephalosporin susceptible), and associated with black race. Pulsotypes were diverse; some were linked to other locales. ST131 emerged significantly during the study period. These findings suggest that E. coli ST131 may constitute an important new multidrug-resistant threat to renal transplant recipients.
&lt;title&gt;Object-based video: extraction tools, evaluation metrics, and applications&lt;/title&gt; (2003)

The representation of video information in terms of its content is at the foundation of many multimedia applications, such as broadcasting, content-based information retrieval, interactive video, remote surveillance and entertainment. In particular, object-based representation consists in decomposing the video content into a collection of meaningful objects. This approach offers a broad range of capabilities in terms of access, manipulation and interaction with the visual content. The basic difference when compared with pixel-based procedures is that instead of processing individual pixels, image objects are used in the representation. To exploit the benefits of object-based representation, multimedia applications need automatic techniques for extracting such objects from video data, a problem that still remains largely unsolved. In this paper, we first review the extraction techniques that enable the separation of foreground objects from the background. Their field of applicability and their limitations are discussed. Next, automatic tools for evaluating their performances are introduced. The major applications that benefit from an object-based approach are then analysed. Finally, we discuss some open research issues in object-based video.
Continuous execution: the VisiProg environment (1985)

To date, program development environments have been static rather than dynamic. Even emerging interactive, integrated program development environments, like the Cornell Program Synthesizer, view program editing and execution as essentially independent activities. We envision an even more dynamic environment in which the functionality (input/output relationship) of a network of programs, an individual program, or a program segment can be viewed “continuously” with editing changes to either the program input or program body. This is the VisiCalc concept extended to program development environments (VisiProg). In this paper, this “dynamic” approach to program development, testing and debugging is addressed, and considerations for the user interface are discussed. The latter includes a workstation with a flexible windowing system, three-dimensional views of programs, insertion of program control and observation points, and dynamic program slicing for “viewing” program execution. An existing prototype and current development activities are also discussed.
Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin (2009)

Bub1 is a multi-task protein kinase required for proper chromosome segregation in eukaryotes. Impairment of Bub1 in humans may lead to chromosomal instability (CIN) or tumorigenesis. Yet, the primary cellular substrate of Bub1 has remained elusive. Here, we show that Bub1 phosphorylates the conserved serine 121 of histone H2A in fission yeast Schizosaccharomyces pombe. The h2a-SA mutant, in which all cellular H2A-S121 is replaced by alanine, phenocopies the bub1 kinase-dead mutant (bub1-KD) in losing the centromeric localization of shugoshin proteins. Artificial tethering of shugoshin to centromeres largely restores the h2a-SA or bub1-KD-related CIN defects, a function that is evolutionally conserved. Thus, Bub1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes.
Desulfurization of High-Sulfur Jet Fuel by π-Complexation with Copper and Palladium Halide Sorbents (2006)

Deep desulfurization of a high-sulfur jet fuel (for fuel cell applications) was achieved by adsorption with π-complexation sorbents. The total sulfur content of JP-5 jet fuel was removed from 1172 ppmw S to below 1 ppmw S. The following sorbents were prepared and tested: CuCl supported on activated carbon (CuCl/AC), PdCl2/AC, PdCl2/Al2O3, and Cu(I)−Y zeolite. Comparison of these sorbents as well as other known sorbents showed that PdCl2/AC had the highest sulfur selectivity and capacity. It was found that significant breakthrough occurred at about 6.0 mL/g for desulfurization of JP-5 by PdCl2/AC. Ab initio molecular orbital computation was performed for the bond energies between different sulfur molecules and these sorbents. For methylated benzothiophene, the main sulfur molecules in jet fuels, the bond energies (and the separation factors for organosulfur/benzene) followed the order PdCl2 > CuCl > Cu−Y. This result was in agreement with the experimental breakthrough data. The spent PdCl2/AC was regenerated with benzene in a static system, and the regenerated sorbent was tested for reuse. The results showed that ∼74 wt % adsorbed sulfur could be desorbed and 72% of the sulfur capacity could be recovered for reuse.
Real‐time automated tracking and trapping system for sperm (2006)

We have developed a microscope system for real-time single sperm tracking with an automated laser tweezers escape power assay. Phase contrast images of swimming sperm are digitized to the computer at video rate. The custom algorithm creates a region of interest centered about a sperm in response to a mouse click and performs all subsequent tasks autonomously. Microscope stage movement responds to feedback from video analysis of swimming sperm to center the sperm with respect to the field of view. For escape power assays, sperm are automatically relocated to the laser trap focus where they are held for a user-defined duration at fixed power, or held as laser power is gradually reduced. The sperm's position is automatically monitored to measure the laser power at which the sperm escapes the trap. Sperm are tracked for extended durations before and after laser trap experiments. Motility measurements including the curvilinear velocity and the absolute position of the sperm relative to the cell chamber are calculated and written to the hard drive at video rate. Experimental throughput is increased over 30 times compared to off-line data analysis. The efficacy of the "track and trap" algorithm is validated through examples and comparisons with the manually collected data.
The N domain of somatic angiotensin-converting enzyme negatively regulates ectodomain shedding and catalytic activity (2005)

sACE (somatic angiotensin-converting enzyme) consists of two homologous, N and C domains, whereas the testis isoenzyme [tACE (testis ACE)] consists of a single C domain. Both isoenzymes are shed from the cell surface by a sheddase activity, although sACE is shed much less efficiently than tACE. We hypothesize that the N domain of sACE plays a regulatory role, by occluding a recognition motif on the C domain required for ectodomain shedding and by influencing the catalytic efficiency. To test this, we constructed two mutants: CNdom-ACE and CCdom-ACE. CNdom-ACE was shed less efficiently than sACE, whereas CCdom-ACE was shed as efficiently as tACE. Notably, cleavage occurred both within the stalk and the interdomain bridge in both mutants, suggesting that a sheddase recognition motif resides within the C domain and is capable of directly cleaving at both positions. Analysis of the catalytic properties of the mutants and comparison with sACE and tACE revealed that the k(cat) for sACE and CNdom-ACE was less than or equal to the sum of the kcat values for tACE and the N-domain, suggesting negative co-operativity, whereas the kcat value for the CCdom-ACE suggested positive co-operativity between the two domains. Taken together, the results provide support for (i) the existence of a sheddase recognition motif in the C domain and (ii) molecular flexibility of the N and C domains in sACE, resulting in occlusion of the C-domain recognition motif by the N domain as well as close contact of the two domains during hydrolysis of peptide substrates.
Inversion of acyclic olefins by the phosphorus betaine method. Scope and limitations (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInversion of acyclic olefins by the phosphorus betaine method. Scope and limitationsE. Vedejs and P. L. FuchsCite this: J. Am. Chem. Soc. 1973, 95, 3, 822–825Publication Date (Print):February 1, 1973Publication History Published online1 May 2002Published inissue 1 February 1973https://pubs.acs.org/doi/10.1021/ja00784a032https://doi.org/10.1021/ja00784a032research-articleACS PublicationsRequest reuse permissionsArticle Views979Altmetric-Citations100LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Rapid Eye Movement Sleep and Hippocampal Theta Oscillations Precede Seizure Onset in the Tetanus Toxin Model of Temporal Lobe Epilepsy (2014)

Improved understanding of the interaction between state of vigilance (SOV) and seizure onset has therapeutic potential. Six rats received injections of tetanus toxin (TeTX) in the ventral hippocampus that resulted in chronic spontaneous seizures. The distribution of SOV before 486 seizures was analyzed for a total of 19 d of recording. Rapid eye movement sleep (REM) and exploratory wake, both of which express prominent hippocampal theta rhythm, preceded 47 and 34%, for a total of 81%, of all seizures. Nonrapid eye movement sleep (NREM) and nonexploratory wake, neither of which expresses prominent theta, preceded 6.8 and 13% of seizures. We demonstrate that identification of SOV yields significant differentiation of seizure susceptibilities, with the instantaneous seizure rate during REM nearly 10 times higher than baseline and the rate for NREM less than half of baseline. Survival analysis indicated a shorter duration of preseizure REM bouts, with a maximum transition to seizure at ∼90 s after the onset of REM. This study provides the first analysis of a correlation between SOV and seizure onset in the TeTX model of temporal lobe epilepsy, as well as the first demonstration that hippocampal theta rhythms associated with natural behavioral states can serve a seizure-promoting role. Our findings are in contrast with previous studies suggesting that the correlations between SOV and seizures are primarily governed by circadian oscillations and the notion that hippocampal theta rhythms inhibit seizures. The documentation of significant SOV-dependent seizure susceptibilities indicates the potential utility of SOV and its time course in seizure prediction and control.
Beef Noodles (2011)

Through an unexpectedly rich bowl of beef noodles, author Li Ang engages and disentangles the complex historical, cultural, and ideological weave of Taiwanese identity. Li Ang's ability to bring such disparate elements into a single story speaks to both her writing as well as culinary prowess. In 2007, “Beef Noodles” was made into a theatrical production performed by le Théâtre de l'Opprimé in Paris, France.
Functions and Regulation of Artemis: A Goddess in the Maintenance of Genome Integrity (2010)

Artemis is a structure-specific endonuclease when associated with and phosphorylated by DNA-dependent protein kinase catalytic subunit. This structure-specific endonuclease is responsible for the resolution of hairpin coding ends in V(D)J recombination. In DNA double-strand break repair, Artemis is implicated in the end-processing step of the non-homologous end-joining (NHEJ) pathway. Recently, we have demonstrated that the involvement of Artemis in NHEJ depends on the type of DNA damage. Interestingly, recent evidence suggests that the end-processing activity is not the only function of Artemis. Indeed, Artemis is rapidly phosphorylated by ataxia telangiectasia mutated in response to DNA damage, and such phosphorylation of Artemis appears to be involved in the regulation of cell cycle checkpoints. These findings suggest that Artemis is a multifunctional protein participating in the maintenance of genome integrity at two distinct levels; one at the end processing step of NHEJ, and the other at the signaling pathway of cell cycle regulation. Therefore, understanding Artemis function may give us profound insights into the DNA repair network. In this review, we summarize the functions and regulation of Artemis.
Short Notices (1971)

Journal Article Short Notices Get access J. R. C. WRIGHT J. R. C. WRIGHT Christ ChurchOxford Search for other works by this author on: Oxford Academic Google Scholar The English Historical Review, Volume LXXXVI, Issue CCCXLI, October 1971, Pages 883-c–884, https://doi.org/10.1093/ehr/LXXXVI.CCCXLI.883-c Published: 01 October 1971
Solvent-free matrix dry-coating for MALDI imaging of phospholipids (2008)

A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-microm stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods.