gebhart/patrepeval huggingface dataset:
[Immunological function of HLA-DQw6 molecules expressed in DQw6 transgenic C57BL/6 mice]. (1991)

HLA-DQw6 transgenic C57BL/6 mice (DQw6-B6) were utilized to define the role of HLA-DQw6 gene product in immune-recognition. To investigate the responsiveness of lymph node cells from C57BL/6 mice (B6) or DQw6-B6 in response to DQw6 molecules expressed in the DQw6-B6, in vitro secondary MLR was performed. The lymph node cells from B6 proliferated in response to spleen cells from DQw6-B6, whereas those from DQw6-B6 did not. These results suggested that DQw6-B6 acquired tolerance to DQw6 molecules. The difference of T cell repertoire between B6 and DQw6-B6 was investigated using mAbs directed against T cell receptor V beta regions, V beta 3, V beta 5, V beta 6, V beta 8, V beta 11 and V alpha 3.2. Although the proportion of V beta 5+ CD8+ T cells and V beta 6+ CD4+ T cells were increased in the B6 anti-DQw6-B6 MLR T cell line, there was no significant difference in the proportion of peripheral T cells expressing each V alpha or V beta region between B6 and DQw6-B6. Both CD4+ and CD8+ long term-cultured T lymphocyte cell lines were generated from lymph node cells of B6 stimulated in vitro by irradiated spleen cells from DQw6-B6. The CD4+ T cell line proliferated in response to spleen cells of DQw6-B6 or L cell transfectant expressing HLA-DQw6 molecules and these responses were completely inhibited by either anti-DQ or anti-CD4 monoclonal antibodies (mAbs) but not by anti I-Ab mAbs. The CD8+ T cell line lysed splenic cells activated by lipopolysaccharide (LPS) from DQw6-B6 spleen cells but not from B6. The CD8+ T cell line also exhibited a cytotoxicity to splenic LPS blast cells from backcross progenies between (DQw6-B6 x DBA1) F1 and DBA1, only when target cells expressed both HLA-DQw6 molecules and H-2b. These observations indicated that recognition of HLA-DQw6 by the CD8+ cytotoxic T cell line was restricted by H-2b. The cytolytic activity of the CD8+ T cell line was inhibited by either anti-CD8 or anti-H-2Db mAbs but not by anti-HLA-DQ nor anti-H-2Kb mAbs. These results show that HLA-DQ transgene products expressed in DQw6-B6 can induce xenogeneic MLR in both CD4+ and CD8+ T cells. The CD4+ T lymphocytes recognize the HLA-DQw6 molecule itself whereas the CD8+ cytotoxic T lymphocytes recognize the HLA-DQw6 gene product in the context of H-2Db.
Probing the Local Density of States of Metal Surfaces by Deexcitation of Metastable Noble-Gas Atoms (1983)

Resonance ionization followed by Auger neutralization is the mechanism for deexcitation of metastable noble-gas atoms (He, Ne, Ar) at metal surfaces which are either clean or covered with "small" atomic adsorbates and whose work function is \ensuremath{\gtrsim} 4.5 eV. The energy distributions of the emitted electrons reflect the self-convolution of the local density of states of the outermost atomic layer and thus provide a spectroscopic technique with extreme surface sensitivity as demonstrated with Pd(111) and Cu(110) surfaces.
Using sequential and non-sequential patterns in predictive Web usage mining tasks (2003)

We describe an efficient framework for Web personalization based on sequential and non-sequential pattern discovery from usage data. Our experimental results performed on real usage data indicate that more restrictive patterns, such as contiguous sequential patterns (e.g., frequent navigational paths) are more suitable for predictive tasks, such as Web prefetching, (which involve predicting which item is accessed next by a user), while less constrained patterns, such as frequent item sets or general sequential patterns are more effective alternatives in the context of Web personalization and recommender systems.
Parkinson’s disease misdiagnosed as stroke (2009)

Parkinson's disease (PD) is a neurodegenerative disease, the clinical features of which are usually asymmetrical at presentation. This can lead to difficulty in differentiating it from other asymmetric neurological disorders. We present two cases where idiopathic PD was initially misdiagnosed as stroke, leading to a delay in appropriate symptomatic therapy. Physicians involved in diagnosis and treatment of people with strokes should consider PD when formulating their differential diagnosis.
LAP, an alcohol acetaldehyde dehydrogenase enzyme in Listeria, promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species (2010)

Listeria adhesion protein (LAP), an alcohol acetaldehyde dehydrogenase (lmo1634), interacts with host-cell receptor Hsp60 to promote bacterial adhesion during the intestinal phase of Listeria monocytogenes infection. The LAP homologue is present in pathogens (L. monocytogenes, L. ivanovii) and non-pathogens (L. innocua, L. welshimeri, L. seeligeri); however, its role in non-pathogens is unknown. Sequence analysis revealed 98 % amino acid similarity in LAP from all Listeria species. The N-terminus contains acetaldehyde dehydrogenase (ALDH) and the C-terminus an alcohol dehydrogenase (ADH). Recombinant LAP from L. monocytogenes, L. ivanovii, L. innocua and L. welshimeri exhibited ALDH and ADH activities, and displayed strong binding affinity (K(D) 2-31 nM) towards Hsp60. Flow cytometry, ELISA and immunoelectron microscopy revealed more surface-associated LAP in pathogens than non-pathogens. Pathogens exhibited significantly higher adhesion (P<0.05) to Caco-2 cells than non-pathogens; however, pretreatment of bacteria with Hsp60 caused 47-92 % reduction in adhesion only in pathogens. These data suggest that biochemical properties of LAP from pathogenic Listeria are similar to those of the protein from non-pathogens in many respects, such as substrate specificity, immunogenicity, and binding affinity to Hsp60. However, protein fractionation analysis of extracts from pathogenic and non-pathogenic Listeria species revealed that LAP was greatly reduced in intracellular and cell-surface protein fractions, and undetectable in the extracellular milieu of non-pathogens even though the lap transcript levels were similar for both. Furthermore, a LAP preparation from L. monocytogenes restored adhesion in a lap mutant (KB208) of L. monocytogenes but not in L. innocua, indicating possible lack of surface reassociation of LAP molecules in this bacterium. Taken together, these data suggest that LAP expression level, cell-surface localization, secretion and reassociation are responsible for LAP-mediated pathogenicity and possibly evolved to adapt to a parasitic life cycle in the host.
The crystal structure of urea oxalic acid (2:1) (1972)

The crystal structure of urea oxalic acid, 2[CO(NH2)2].(COOH)2 has been determined using three-dimensional X-ray data, collected on an automatic diffractometer. The space group is P21/c. The lattice constants are: a = 5.058 (3), b = 12.400 (3), c = 6.964 (2) A, fl= 98"13 (7) °. The number of molecules in the unit cell is two. The structure consists of layers of urea and oxalic acid molecules held together by hydrogen bonds. The positions of all hydrogen atoms have been found. The compound is not a uronium salt.
<i>Thyroid Cancer: A Comprehensive Guide to Clinical Management</i> (2000)

Dedicated to the memory of a great thyroidologist, teacher, and friend, Dr
<i>α</i>-tocopherol consumption during low-density-lipoprotein oxidation (1990)

1. The kinetics of the depletion of alpha-tocopherol in human low-density lipoprotein (LDL) were measured during macrophage-mediated and cell-free oxidation. The formation of oxidatively modified, high-uptake species of LDL in these systems was not detectable until all of the endogenous alpha-tocopherol had been consumed. 2. Supplementation of the alpha-tocopherol content of LDL by loading in vivo extended the duration of the lag period during which no detectable oxidative modification occurred. 3. The addition of a flavonoid (morin) prevented both alpha-tocopherol consumption and oxidative modification of LDL. 4. The alpha-tocopherol contents of LDLs from a range of individual donors could not be used to predict their relative resistance to oxidation, indicating that other endogenous antioxidants may also be present, and quantitatively significant, in human LDL.
Realistic modeling and rendering of plant ecosystems (1998)

Modeling and rendering of natural scenes with thousands of plants poses a number of problems. The terrain must be modeled and plants must be distributed throughout it in a realistic manner, reflecting the interactions of plants with each other and with their environment. Geometric models of individual plants, consistent with their positions within the ecosystem, must be synthesized to populate the scene. The scene, which may consist of billions of primitives, must<br />be rendered efficiently while incorporating the subtleties of lighting in a natural environment.<br /><br />We have developed a system built around a pipeline of tools that address these tasks. The terrain is designed using an interactive graphical editor. Plant distribution is determined by hand (as one would do when designing a garden), by ecosystem simulation, or by a combination of both techniques. Given parametrized procedural models of individual plants, the geometric complexity of the scene is<br />reduced by approximate instancing, in which similar plants, groups of plants, or plant organs are replaced by instances of representative objects before the scene is rendered. The paper includes examples of visually rich scenes synthesized using the system.
Ultrasensitive Detection of DNA in Diluted Serum Using NaBH<sub>4</sub> Electrooxidation Mediated by [Ru(NH<sub>3</sub>)<sub>6</sub>]<sup>3+</sup> at Indium−Tin Oxide Electrodes (2010)

There is a crucial need for simple and highly sensitive techniques to detect DNA in complicated biological samples such as serum. Here we present an ultrasensitive electrochemical DNA sensor using (i) single DNA hybridization with peptide nucleic acid (PNA), (ii) selective binding of [Ru(NH(3))(6)](3+) to hybridized DNA, (iii) fast NaBH(4) electrooxidation mediated by [Ru(NH(3))(6)](3+), and (iv) low background currents of NaBH(4) at indium-tin oxide (ITO) electrodes. The [Ru(III)(NH(3))(5)NH(2)](2+) formed from [Ru(III)(NH(3))(6)](3+) in borate buffer (pH 11.0) is readily electrooxidized to both [Ru(IV)(NH(3))(5)NH(2)](3+) and Ru complex with a higher oxidation state. In the absence of [Ru(NH(3))(6)](3+) bound to the DNA-sensing ITO electrodes, the oxidation currents of NaBH(4) are very low. However, in the presence of [Ru(NH(3))(6)](3+), the oxidation currents of NaBH(4) are highly enhanced due to electron mediation of the oxidized Ru complexes. The significant enhancement in the electrocatalytic activity of sensing electrodes after [Ru(NH(3))(6)](3+) binding facilitates to obtain high signal-to-background ratios. PNA and ethylenediamine on DNA-sensing electrodes significantly decrease [Ru(NH(3))(6)](3+) binding, also allowing for high signal-to-background ratios. The oxidation charges of NaBH(4) obtained from chronocoulometry are highly reproducible. All combined effects enable the detection of DNA with a detection limit of 1 fM in ten-fold diluted human serum. The simple and fast detection procedure and the ultrasensitivity make this approach highly promising for practical DNA detection.
Interaction between fibrinogen and cultured endothelial cells. Induction of migration and specific binding. (1985)

It has been suggested that fibrinogen (fg) or its physiological derivatives influence the motility and growth of endothelial cells (ECs), but direct support for this concept is still lacking. In the present study, the capacity of fg to interact with ECs and induce the migration of ECs was examined. The capacity of fg to induce EC migration was studied by means of a modification of the Boyden chamber technique. fg in the lower compartment of the chamber caused a time- and concentration-dependent migration of ECs across filters. fg present in equal concentrations above and below the filter increased EC migration, but the maximal effect invariably occurred in the presence of a gradient between the lower and the upper compartments. Trypsin or plasmin digestion of fg and preincubation of fg with Fab fragments from specific antibody completely abolished fg-induced EC migration. Dialysis of fg to eliminate small peptides that might contaminate the preparation did not modify fg-induced migration. Plasma obtained from healthy donors induced EC migration, but plasma from an afibrinogenemic patient was completely ineffective. The addition of purified fg to afibrinogenemic plasma restored plasma-induced EC migration. Plasmin degradation fragments D and E, of 100,000 and 50,000 mol wt, respectively, did not induce EC migration. However, fragment E caused dose-related inhibition of fg-induced EC migration Direct interaction of highly purified radioiodinated human fg with cultured human and bovine Ecs was observed. The binding was time dependent and plateaued at 10 min. Nonlabeled fg in a large molar excess inhibited the interaction, but unrelated proteins, including fibronectin, ovalbumin, and myoglobin, did not. Monospecific Fab fragments directed to fg inhibited binding by 38% at a 50 to 1 molar ratio whereas nonimmune Fab caused only 2% inhibition at a similar concentration. The binding of 125I-fg with ECs was saturable, and an apparent dissociation constant of 0.23 x 10(-6) M was estimated from binding isotherms. After 30 min of incubation the interaction between 125I-fg and the cells was completely reversible and displaceable by a large molar excess of unlabeled fg. Autoradiography of the display of EC-bound 125I on polyacrylamide gel showed the constitutive B beta- and gamma-chains of the fg molecule, with a partial loss of the A alpha-chain. Purified fragment E and E were tested for their capacity to inhibit fg binding. At a 1 to 400 125I-fg-to-fragment molar ratio, fragment E, which also inhibited migration, competed for binding by 44%, but fragment D was completely ineffective. These data show that fg may specifically associate with ECs and induce migration of these cells; it also appears that the structural requirement of this activity is located in the N-terminal part of the molecule.
DESIGNING WITH THE SHAPE MEMORY EFFECT (1989)

Some of the engineering aspects of shape memory product design are reviewed, with the emphasis on product screening. After a short general description of the memory effect, applications are categorized according to the func tion of the memory alloys: to cause motion, to generate a stress, to do work, or to store elastic energy. Each of these functions is overviewed, with schematic and practical examples, typical design problems, examples of relevant design data, alloy selection criteria, and a guideline for screening new applications.
Treatment Strategies after SSRI Failure — Good News and Bad News (2006)

My recent use of that august medical search engine, Google, revealed a publication that identified sin as the cause of depression. Initial amusement quickly gave way to the sobering recognition that this simplistic formulation conveyed a prevalent, if generally unarticulated, belief that depression is not a real illness but, rather, the result of a personal failing — moral, spiritual, or adaptational. How did depression come to be viewed in so pejorative a manner?Depression certainly cannot be dismissed as rare; its lifetime prevalence is 15 to 20 percent1 (as compared, for example, with a 4 to 6 percent lifetime prevalence . . .
Stereotactic multiple arc radiotherapy (1990)

A radiotherapy technique is presented for delivering a concentrated pattern of absorbed dose to intracranial lesions. Treatment takes place on a conventional, isocentrically mounted linear accelerator, rotated in several planes around a single target site. A new, relocatable stereotactic frame is used which enables fractionated radiotherapy to be administered if required. Calculations of the absorbed dose distribution in three orthogonal planes are performed using specially prepared software on a computer used for standard treatment planning. In this way, the need for excessive computing power is avoided.
Spermatogenesis (1998)

Spermatogenesis is a complex process involving mitotic cell division, meiosis and the process of spermiogenesis. The regulation of spermatogenesis involves both endocrine and paracrine mechanisms. The endocrine stimulation of spermatogenesis involves both follicle stimulating hormone (FSH) and luteinizing hormone, the latter acting through the intermediary testosterone, produced by the Leydig cells in the testis. Since the germ cells do not possess receptors for FSH and testosterone, the hormonal signals are transduced through the Sertoli cells and peritubular cells by the production of signals that have yet to be defined. Although the hormonal signals are essential for successful spermatogenesis, there is increasing evidence that a multiplicity of growth factors and cytokines are involved in local control mechanisms influencing stem cell renewal by mitosis and the complicated process of the two meiotic cell divisions. The final complex metamorphosis which converts a round cell into the complex structures of the spermatozoa is well defined at a structural level, but the control systems regulating this process still remain to be elucidated.
Anatomical analysis of the femoral condyle in normal and osteoarthritic knees (2003)

It is important to understand anatomical feature of the distal femoral condyle for treatment of osteoarthritic knees. Detailed measurement of the femoral condyle geometry, however, has not been available in osteoarthritic knees including valgus deformity. This study evaluated femoral condyle geometry in 30 normal knees, 30 osteoarthritic knees with varus deformity, and 30 osteoarthritic knees with valgus deformity using radiographs and magnetic resonance imaging (MRI). In radiographic analysis in the coronal plane, the femoral joint angle (lateral angle between the femoral anatomic axis and a tangent to femoral condyles) was 83.3 degrees in the normal knees, 83.8 degrees in the varus knees, and 80.7 degrees in the valgus knees. In MRI analysis in the axial plane, the posterior condylar tangent showed 6.4 degrees of internal rotation relative to the transepicondylar axis in the normal knees, 6.1 degrees in the varus knees, and 11.5 degrees in the valgus knees. These results suggested that there was no hypoplasia of the medial condyle in the varus knees, but the lateral condyle in the valgus knees was severely distorted. Surgeons should take this deformity of the lateral femoral condyle into account when total knee arthroplasty is performed for a valgus knee.
Pyruvate: Metabolic Protector of Cardiac Performance (2000)

Pyruvate, a metabolic product of glycolysis and an oxidizable fuel in myocardium, increases cardiac mechanical performance and energy reserves, especially when supplied at supraphysiological concentrations. The inotropic effects of pyruvate are most impressive in hearts that have been reversibly injured (stunned) by ischemia/reperfusion stress. Glucose appears to be an essential co-substrate for pyruvate's salutary effects in stunned hearts, but other fuels including lactate, acetate, fatty acids, and ketone bodies produce little or no improvement in postischemic function over glucose alone. In contrast to pharmacological inotropism by catecholamines, metabolic inotropism by pyruvate increases cardiac energy reserves and bolsters the endogenous glutathione antioxidant system. Pyruvate enhancement of cardiac function may result from one or more of the following mechanisms: increased cytosolic ATP phosphorylation potential and Gibbs free energy of ATP hydrolysis, enhanced sarcoplasmic reticular calcium ion uptake and release, decreased cytosolic inorganic phosphate concentration, oxyradical scavenging via direct neutralization of peroxides and/or enhancement of the intracellular glutathione/NADPH antioxidant system, and/or closure of mitochondrial permeability transition pores. This review aims to summarize evidence for each of these mechanisms and to consider the potential utility of pyruvate as a therapeutic intervention for clinical management of cardiac insufficiency.
Variability and reproducibility of hepatic FDG uptake measured as SUV as well as tissue‐to‐blood background ratio using positron emission tomography in healthy humans (2008)

In normal subjects hepatic FDG-uptake is high and homogeneous with a low CV% between days. T/B corrected values are largely comparable to SUV values but not superior, probably due to the standardization of procedures and homogeneity of the subjects. The T/B corrected method is theoretically superior in a more inhomogeneous population or when using different scanners and is shown here to be easy to apply. Small ROIs of 10 cm(3) are representative with respect to mean FDG uptake in the total liver and reproducibility, but do not identify the max FDG uptake.
Anodic Formation of Oxide Films on Silicon (1957)

Dense oxide films have been formed anodically on p‐ and n‐type single‐crystal Si in connection with the electrical properties of Si surfaces. Concentrated or permit forming, but the highest voltage obtainable is less than 200 v. A solution of in N‐methylacetamide permits forming to 560 v, and is also preferable in other respects.
.mu.-.eta.2:.eta.2-Peroxo binuclear copper complex, [Cu(HB(3,5-(Me2CH)2pz)3)]2(O2) (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT.mu.-.eta.2:.eta.2-Peroxo binuclear copper complex, [Cu(HB(3,5-(Me2CH)2pz)3)]2(O2)Nobumasa Kitajima, Kiyoshi Fujisawa, Yoshihiko Morooka, and Koshiro ToriumiCite this: J. Am. Chem. Soc. 1989, 111, 24, 8975–8976Publication Date (Print):November 1, 1989Publication History Published online1 May 2002Published inissue 1 November 1989https://pubs.acs.org/doi/10.1021/ja00206a062https://doi.org/10.1021/ja00206a062research-articleACS PublicationsRequest reuse permissionsArticle Views1842Altmetric-Citations346LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Inhibiting the hyperreflexic bladder with electrical stimulation in a spinal animal model (1993)

Uninhibited bladder contractions are a problem in spinal cord injured patients. Accordingly, methods using electrical stimulation to inhibit the bladder were investigated in chronic spinal cord injured (C6-T1) male cats. In unanesthetized, restrained animals, spontaneous bladder contractions were observed after the bladder was filled above the micturition threshold. In 3 of the 5 cats studied, this bladder activity could be inhibited with stimulation of either sacral nerves or pudendal nerves. Pudendal nerve stimulation, however, was more selective than sacral nerve stimulation for inhibition with fewer side effects such as leg spasms. Tibial nerve stimulation was ineffective and caused leg spasms and increased bladder activity. Finally, high-frequency stimulation (1,000 Hz) of the sacral nerves was shown to block bladder contractions in 2 of 3 cats investigated. However, this method had adverse side effects such as leg flexion and secondary bladder contractions. We conclude that pudendal nerve/pelvic floor stimulation at low frequency is a relatively effective method in this model.