gebhart/patrepeval huggingface dataset:
FLT3 Ligand Preserves the Ability of Human CD34+ Progenitors to Sustain Long-Term Hematopoiesis in Immune-Deficient Mice After Ex Vivo Retroviral-Mediated Transduction (1997)

Stromal support is required during retroviral-mediated transduction of human bone marrow-derived CD34+ cells to maintain the clonogenicity of the primitive progenitors. We hypothesized that the cytokine FLT3 ligand (FL) might be able to replace the maintenance role provided by the stroma. CD34+ progenitors from human bone marrow were transduced by the retroviral vector LN with the cytokines interleukin-3 (IL-3), IL-6, and stem cell factor (SCF) present in all cultures. Transductions were performed with or without stromal support and with or without the inclusion of 100 U/mL FL. No significant increase in gene transfer into colony-forming cells was obtained by the addition of FL to the cultures. Transduction and survival of more primitive human hematopoietic cells was determined by growth in immune-deficient mice for 7 to 8 months. Human myeloid cells, T lymphocytes, and colony-forming progenitors were recovered from the marrow of mice that had received human cells transduced on stroma or in suspension culture with IL-3, IL-6, SCF, and FL, but not with IL-3, IL-6, and SCF alone. LN provirus was detected by polymerase chain reaction in the marrow recovered from 9 of 10 mice transplanted with human CD34+ cells transduced with stromal support, 5 of 11 mice that received human cells transduced in suspension culture with FL, but none of the 10 mice that received human cells transduced in suspension culture without FL We conclude that FLT3 ligand, in conjunction with IL-3, IL-6, and SCF, preserves the generative capacity of primitive human hematopoietic cells during in vitro transductions in suspension culture.
Efficient Progressive Skyline Computation (2001)

In this paper, we focus on the retrieval of a set of interesting answers called the skyline from a database. Given a set of points, the skyline comprises the points that are not dominated by other points. A point dominates another point if it is as good or better in all dimensions and better in at least one dimension. We present two novel algorithms, Bitmap and Index, to compute the skyline of a set of points. Unlike most existing algorithms that require at least one pass over the dataset to return the rst interesting point, our algorithms progressively return interesting points as they are identi ed. Our performance study further shows that the proposed algorithms provide quick initial response time with Index being superior in most cases.
Interference mitigation in transmitted-reference ultra-wideband (UWB) receivers (2004)

Transmitted-reference (TR) ultra-wideband transceivers have recently become increasingly popular for their simplicity, capability to reduce the stringent UWB timing requirements, and robust performance in multipath channels. However, the performance of TR receivers is considerably limited by the severity of noise-on-noise component introduced by various types of channel noise such as additive white Gaussian noise (AWGN) or narrowband interference (NBI) on the transmitted signal. It is expected that such receivers will perform poorly at low signal-to-noise ratio links, or in the presence of strong narrowband interferers. In this paper we propose a novel technique that maximizes the extraction of information from reference pulses for UWB-TR receivers. The scheme efficiently processes the incoming signal to suppress different types of interference prior to signal detection. The method described introduces a feedback loop mechanism to enhance the signal-to-noise ratio of reference pulses in a conventional TR receiver. The performance of a conventional TR receiver and a feedback loop TR receiver in the presence of AWGN and strong narrowband interference is investigated by analysis and computer simulations. Our studies show that the reference enhancing feedback loop mechanism greatly improves the robustness of the link performance of TR receivers in the presence of non-UWB interference with modest increase in complexity.
Optical metastability in bulk GaN single crystals (1997)

Bulk GaN single crystals were grown from cold pressed GaN powder by sublimation in flowing ammonia. Optical transmission measurements indicated that the absorption coefficient for the transparent samples is 50 cm−1 in the wavelength region from 650 to 400 nm. Optical metastability in bulk GaN crystals was studied through time dependent photoluminescence both at room and liquid–nitrogen temperatures. The observation included decreasing output intensity of the ultraviolet emission attributed to the band edge and increasing output intensity of a new emission band centered at 378 nm at room temperature. At liquid–nitrogen temperature, the photoinduced emission band consisted of at least one LO-phonon replica of the zero-phonon line centered at 378 nm. The ratio of output intensities of the photoinduced band to the band edge increased by a factor of 10 during 27 min of exposure time. The photoinduced effect is attributed to the metastable nature of traps in bulk GaN.
Measuring the performance of communication middleware on high-speed networks (1996)

Conventional implementations of communication middleware (such as CORBA and traditional RPC toolkits) incur considerable over-head when used for performance-sensitive applications over high-speed networks. As gigabit networks become pervasive, inefficient middleware will force programmers to use lower-level mechanisms to achieve the necessary transfer rates. This is a serious problem for mission/life-critical applications (such as satellite surveillance and medical imaging).This paper compares the performance of several widely used communication middleware mechanisms on a high-speed ATM network. The middleware ranged from lower-level mechanisms (such as socket-based C interfaces and C++ wrappers for sockets) to higher-level mechanisms (such as RPC, hand-optimized RPC and two implementations of CORBA - Orbix and ORBeline). These measurements reveal that the lower-level C and C++ implementations outperform the CORBA implementations significantly (the best CORBA throughput for remote transfer was roughly 75 to 80 percent of the best C/C++ throughput for sending scalar data types and only around 33 percent for sending structs containing binary fields), and the hand-optimized RPC code performs slightly better than the CORBA implementations. Our goal in precisely pinpointing the sources of overhead for communication middleware is to develop scalable and flexible CORBA implementations that can deliver gigabit data rates to applications.
Hybrid Multi-level Cache Management Policy (2014)

Recently, multi-level cache became more popular due to its better performance than single level cache. It is very useful in distributed and parallel systems where number of applications is running at a time. The data required by application program is easily available in the cache due to larger size of the cache. Until now, many multi-level cache management policies LRU-K [15], PROMOTE [1], DEMOTE [5] has been developed but still there is performance issue. The main difficulty of these policies is selecting a victim. In this paper, a new policy is suggested which uses compressed caching and selects a victim depending upon three factors. First is how many times the promotion or the demotion of the cache block has done, second is size of the cache block to be replaced, and the third is recency of the block in the cache memory[1]. This policy is expected to exhibit better hit ratio than all the previously existing multi-level cache management policies.
A hybrid AlGaInAs-silicon evanescent preamplifier and photodetector (2007)

We report the integration of a hybrid silicon evanescent waveguide photodetector with a hybrid silicon evanescent optical amplifier. The device operates at 1550 nm with a responsivity of 5.7 A/W and a receiver sensitivity of -17.5 dBm at 2.5 Gb/s. The transition between the passive silicon waveguide and the hybrid waveguide of the amplifier is tapered to increase coupling efficiency and to minimize reflections.
4-Bits digitally driven integrated amplitude modulator for data processing (1980)

A digitally driven integrated-optical amplitude modulator, acting as a digital-analogue convenor, has been fabricated on a LiNbO3 substrate. It can be driven by 4-bits digital circuitry with a command voltage of 2.6 V.
Design and synthesis of model and practical palladium catalysts using atomic layer deposition (2016)

We investigated the “one-batch” synthesis of model and practical palladium catalysts using atomic layer deposition (ALD).
Color designations for lights (1943)

An extension of the ISCC-NBS (Inter-Society Color Council-National Bureau of Standards) system of color names for the description of the colors of drugs and medicines, has been made for describing the colors of lights. The color names consist of hue names, such as red, pink, yellowish green, or purple, without further modifiers, since they are intended to differentiate lights chiefly according to hue. The hue names are among those used in the ISCC-NBS system and carry the same meaning. The chromaticity ranges identified by each of these hue names are defined by areas on the ICI chromaticity diagram. Comparisons are made between the centers of the proposed hue-name ranges and similar values by other authorities, and with the standard colors recognized in various specifications for marine, railway, aviation, and traffic signal colors.
Hepatic Arterial Administration of Activated Leukocytes in Patients with Liver Metastases (2002)

HAI is a technically feasible way of regional delivery of a high number of activated leukocytes obtained after short ex vivo activation.
Homocystinuria: An Enzymatic Defect (1964)

A deficiency, or absence, of cystathionine synthetase activity has been demonstrated in liver obtained from a mentally retarded child with homocystinuria.
SPC3042: a proapoptotic survivin inhibitor (2008)

The ability to regulate the cellular homeostasis of a higher organism through tight control of apoptosis and cell division is crucial for life. Dysregulation of these mechanisms is often associated with cancerous phenotypes in cells. Optimal cancer therapy is a fine balance between effective cancer cell killing and at the same time minimizing, or avoiding, damage to the surrounding healthy tissue. To obtain this, it is necessary to identify and inhibit molecular targets on which the cancer cells are strongly dependent. Survivin represents such a target, and it has been published previously that peptide vaccines, the small-molecule YM155, and the antisense molecule LY2181308/ISIS23722, via different mechanisms, have been used as survivin inhibitors. In this article, a new potent antisense inhibitor of survivin, SPC3042, is presented, and the properties of SPC3042 are compared with the previously published antisense drug, LY2181308/ISIS23722. SPC3042 is a 16-mer locked nucleic acid (LNA) oligonucleotide and designed as a fully phosphorothiolated gapmer containing 7 LNA nucleotides in the flanks. The LNA nucleotides in SPC3042 provide nuclease stability and higher potency for survivin mRNA inhibition compared with earlier generations of antisense reagents. It is shown that the down-regulation of survivin with SPC3042 leads to cell cycle arrest, pronounced cellular apoptosis, and down-regulation of Bcl-2. It is also shown that SPC3042 is a sensitizer of prostate cancer cells to Taxol treatment in vitro and in vivo.
An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene (2001)

H1 RNA, the RNA component of the human nuclear RNase P, is encoded by a unique gene transcribed by RNA polymerase III (Pol III). In this work, cis-acting elements and trans-acting factors involved in human H1 gene transcription were characterized by transcription assays of mutant templates and DNA binding assays of recombinant proteins. Four elements, lying within 100 bp of 5'-flanking sequences, were defined to be essential for maximal in vitro and in vivo expression, consisting of the octamer, Staf, proximal sequence element (PSE) and TATA motifs. These are also encountered in the promoter elements of vertebrate snRNA genes, where the first two constitute the distal sequence element (DSE). In all the genes examined so far, the DSE is distant from the PSE and TATA box that compose the basal promoter. However, we observed a fundamental difference in the organization of the H1 RNA and snRNA gene promoters with respect to the relative spacing of the DSE and PSE. Indeed, the H1 promoter is unusually compact, with the octamer motif and Staf binding site adjacent to the PSE and TATA motifs. It thus appears that the human RNase P RNA gene has adopted a unique promoter strategy placing the DSE immediately adjacent to the basal promoter.
CCI and CI Join Hands:A Better Supply Chain with More Innovations on Cotton Fabrics (2010)

Cotton Council International('CCI')and Cotton Incorporated('CI') joined forces again,from October 19-22,2010 at Intertextile Shanghai,to promote natural fiber-U.S.cotton.As global textile strategic partners,both organizations were bringing together alliances through the cotton
Digital Photoelasticity: Principles, Practice and Potential (2002)

Abstract The enormously enhanced power of photoelasticity resulting from adoption of digital technologies is highlighted and discussed. An overview of the principal techniques of digital fringe processing is provided within a single theoretical framework. The practical application of the new technologies using both conventional instruments and novel optical devices is discussed. Experiments involving more 1 × 10 6 quantitative fringe order measurements are possible and practical on a routine basis using the current technology. Products based on this research are beginning to appear on the market so that many new application areas are opening up for photoelasticity, such as dynamic events, real‐time fatigue crack analysis, monitoring polarisation changes at a microscopic level in materials; detailed validation of numerical simulations, particularly of complex geometry and loading; and in‐service monitoring using reflection photoelasticity of damage in both homogeneous and heterogeneous materials, such as composites.
CU2CL: A CUDA-to-OpenCL Translator for Multi- and Many-Core Architectures (2011)

The use of graphics processing units (GPUs) in high-performance parallel computing continues to become more prevalent, often as part of a heterogeneous system. For years, CUDA has been the de facto programming environment for nearly all general-purpose GPU (GPGPU) applications. In spite of this, the framework is available only on NVIDIA GPUs, traditionally requiring reimplementation in other frameworks in order to utilize additional multi- or many-core devices. On the other hand, OpenCL provides an open and vendor-neutral programming environment and runtime system. With implementations available for CPUs, GPUs, and other types of accelerators, OpenCL therefore holds the promise of a "write once, run anywhere" ecosystem for heterogeneous computing. Given the many similarities between CUDA and OpenCL, manually porting a CUDA application to OpenCL is typically straightforward, albeit tedious and error-prone. In response to this issue, we created CU2CL, an automated CUDA-to-OpenCL source-to-source translator that possesses a novel design and clever reuse of the Clang compiler framework. Currently, the CU2CL translator covers the primary constructs found in CUDA runtime API, and we have successfully translated many applications from the CUDA SDK and Rodinia benchmark suite. The performance of the automatically translated applications via CU2CL is on par with their manually ported counterparts.
Consequences of altered isoprenylation targets ona-factor export and bioactivity. (1994)

Cysteine-containing amino acid sequences (CAAX, CC, and CXC; C is cysteine, A is any aliphatic amino acid, and X is any amino acid) are targets for the attachment of C15 (farnesyl) and C20 (geranylgeranyl) isoprenoids to peptides and proteins by specific prenyltransferases. Although much work has centered on the enzymatic mechanisms of these enzymes, the biological consequences of the differential isoprenylation they catalyze remain to be elucidated. Farnesylation of the a-factor mating pheromone of Saccharomyces cerevisiae is a known prerequisite for its biological activity and its secretion through a pathway utilizing the yeast STE6 protein, a homolog of the mammalian multidrug resistance (MDR) P-glycoprotein. We generated specific mutations in the a-factor gene to encode isoprenylation targets for geranylgeranylation [Cys-Val-Ile-Leu (CVIL) and Ser-Val-Cys-Cys (SVCC)] in place of the natural farnesylation motif [Cys-Val-Ile-Ala (CVIA)]. The a-factors containing these modified prenylation sites were successfully exported by a STE6-dependent mechanism. Furthermore, these peptides, as well as synthetic geranylgeranyl a-factor, retained bioactivity. Chromatographic comparisons of synthetic and biosynthetic pheromones suggest that, in vivo, a peptide substrate containing the geranylgeranylation target CVIL can be both farnesylated and geranylgeranylated. These results clearly demonstrate that in vivo (i) different prenyltransferases may recognize the same substrate; (ii) both farnesylated and geranylgeranylated a-factor peptides are substrates for export via STE6, a MDR-like protein; and (iii) farnesylated and geranylgeranylated pheromones are both biologically active.
Krüppel-like factor 8 regulates VEGFA expression and angiogenesis in hepatocellular carcinoma (2018)

Tumor angiogenesis plays a critical role in hepatocellular carcinoma (HCC) development and progression, but its mechanism is unclear. Krüppel-like factor 8 (KLF8) is a transcription factor that plays an important role in HCC progression. Here, we investigated the role of KLF8 in angiogenesis in HCC and its possible mechanism. Immunohistochemistry, quantitative RT-PCR, western blotting, promoter reporter assays, chromatin immunoprecipitation (ChIP), and chicken chorioallantoic membrane (CAM) and nude mouse tumor models were used to show that the mRNA and protein expression levels of KLF8 and VEGFA are highly correlated in HCC tissue samples. The up-regulation of KLF8 increased VEGFA protein levels and induced VEGFA promoter activity by binding to the CACCC region of the VEGFA promoter. In addition, KLF8 regulated HIF-1α and Focal adhesion kinase (FAK) expression. The PI3K/AKT inhibitor LY294002 inhibited KLF8-induced VEGFA expression, whereas PI3K/AKT signaling pathway proteins, such as P-PDK1(Ser241) and P-AKT(Thr308), were decreased significantly. KLF8-overexpressing HCC cells had a higher potential for inducing angiogenesis. Thus, our results indicate that KLF8 may induce angiogenesis in HCC by binding to the CACCC region of the VEGFA promoter to induce VEGFA promoter activity and through FAK to activate PI3K/AKT signaling to regulate HIF-1α expression levels.
MicroRNA-200a serves a key role in the decline of progesterone receptor function leading to term and preterm labor (2012)

During pregnancy, uterine quiescence is maintained by increased progesterone receptor (PR) activity, but labor is facilitated by a series of events that impair PR function. Previously, we discovered that miR-200 family members serve as progesterone (P(4))-modulated activators of contraction-associated genes in the pregnant uterus. In this study, we identified a unique role for miR-200a to enhance the local metabolism of P(4) in myometrium and, thus, decrease PR function during the progression toward labor. miR-200a exerts this action by direct repression of STAT5b, a transcriptional repressor of the P(4)-metabolizing enzyme 20α-hydroxysteroid dehydrogenase (20α-HSD). We observed that miR-200a expression increased and STAT5b expression coordinately decreased in myometrium of mice as they progressed to labor and in laboring myometrium from pregnant women. These changes were associated with a dramatic increase in expression and activity of 20α-HSD in laboring myometrium from mouse and human. Notably, overexpression of miR-200a in cultured human myometrial cells (hTERT-HM) suppressed STAT5b and increased 20α-HSD mRNA levels. In uterine tissues of ovariectomized mice injected with P(4), miR-200 expression was significantly decreased, STAT5b expression was up-regulated, and 20α-HSD mRNA was decreased, but in 15 d postcoitum pregnant mice injected with the PR antagonist RU486, preterm labor was associated with increased miR-200a, decreased STAT5b, and enhanced 20α-HSD expression. Taken together, these findings implicate miR-200a as an important regulator of increased local P(4) metabolism in the pregnant uterus near term and provide insight into the importance of miR-200s in the decline in PR function leading to labor.
Association of the Mycobacterial 30‐kDa Region Proteins with the Cutaneous Infiltrates of Leprosy Lesions (1992)

The granulomatous skin lesions of human leprosy are known to be due to the cutaneous immune reaction to various mycobacterial antigens. In the present study, by immunohistochemical analysis using a previously characterized monoclonal antibody (MoAb) 3A8 we have demonstrated a selective expression of the 3A8 epitope of mycobacterial 30-kDa proteins, the major secreted proteins of mycobacteria, in various forms of leprosy lesions across the clinical spectrum. The localization of MoAb 3A8 staining is confined to the areas of cellular infiltrates of the lesions. In tuberculoid lesions the intense 3A8 staining was seen mostly in association with the membrane of the dermal cellular infiltrates whereas in highly bacilliferous lepromatous lesions the staining seems to be diffused with granular appearance but not in the form of bacteria. In patients with reversal reaction the staining was specifically extended to cells infiltrating the epidermis. MoAb 3A8 did not show any reactivity with inflammatory skin lesions of patients other than those with leprosy. Since the 3A8 epitope of 30-kDa proteins has been shown to be present in all cellular compartments of the mycobacteria and in the actively secreted BCG 85 antigen complex, MoAb 3A8 reactive protein(s) in leprosy lesions may be derived either from degraded somatic mycobacterial products or from antigens actively secreted by live bacilli. The latter could be true in the cases of untreated lepromatous lesions with high bacterial load since live M. leprae has also been considered to secrete corresponding 30-kDa proteins similar to other closely related mycobacteria. By double immunoenzyme staining we clearly demonstrate the expression of 3A8 epitope on CD68+ macrophages in the granulomas of tuberculoid leprosy, whereas in highly bacilliferous lepromatous lesions most of the double staining was seen in a diffuse pattern within the interstitial space of the cellular infiltrate as well as in the cytoplasm of CD68+ macrophages. In lesions from reversal reaction the 3A8 epitope is more strongly expressed on CDla+ dendritic Langerhans cells (LC) both in the epidermis and in the dermis as compared with other types of leprosy. This provides evidence for the involvement of LC in handling of mycobacterial antigenic epitopes in leprosy lesions. Further, immunoenzyme double staining revealed that the expression of this mycobacterial 3A8 epitope on antigen presenting cells such as CD68+ macrophages and CDla+ LC is present in juxtaposition with CD3+ T cells including the alpha beta and gamma delta receptor-bearing T cells in the granuloma.(ABSTRACT TRUNCATED AT 400 WORDS)