gebhart/patrepeval huggingface dataset:
New method for exposing mammalian cells to intense laser radiation using the evanescent fields created in optical waveguides (1978)

A new method has been developed for exposing mammalian cells to intense laser radiation, using optical waveguides. Attached cells are exposed to the intense evanescent tail associated with the propagating waveguide modes. This technique allows a monolayer of attached cells covering a wide area on the waveguide surface to be exposed to optical power densities in the 105–108 W/cm2 range. Waveguide modes containing power densities in this range are easily excited by moderate-power continuous-wave (CW) lasers. A monolayer consisting of an asynchronous population of 105 EMT-6 cells was plated over a 2-cm2 area on the top surface of an optical waveguide. This waveguide was fabricated by rf sputtering Ba−glass on a glass microscope slide used as a substrate. The area occupied by the cells was defined by a cell chamber constructed on top of the waveguide and filled with alpha-MEM growth medium. TE0 and TE1 waveguide modes were excited in the waveguide using a single mode (TEM00 and single longitudinal mode) CW Ar+-ion laser at a wavelength of 5145 Å. Only 33.7% of the cells survived when the maximum power density and electric field strength within the waveguide reached 4×105 W/cm2 and 104 V/cm, respectively. No appreciable absorption of the laser radiation was detected due to the presence of the cells or nutrient growth medium and no significant temperature rise was noticed at the waveguide-cell interface. It is strongly suggested that the cell-killing mechanism is directly related to both the intense electric field at the cell-waveguide interface and the penetration of the evanescent tail into the cell. Interactions which can only take place in the presence of intense electric field strengths at optical frequencies are presented as possible mechanisms involved in the cell-killing process. Procedures are outlined for the design of waveguides capable of producing optical power densities and electric field strengths up to 108 W/cm2 and 105 V/cm, respectively.
Three-Dimensional Sound Field Reproduction Using Multiple Circular Loudspeaker Arrays (2010)

Three-dimensional spatial sound field reproduction enables enhanced immersive acoustic experience for a listener. Recreating an arbitrary 3-D spatial sound field using a practically realizable array of loudspeakers is a challenging problem in acoustic signal processing. This paper exploits the underlying characteristics of wavefield propagation to devise a strategy for accurate 3-D sound field reproduction inside a 3-D region of interest with practical array geometries. Specifically, we use the properties of the associated Legendre functions and the spherical Hankel functions, which are part of the solution to the wave equation in spherical coordinates, for loudspeaker placement on a set of multiple circular arrays and provide a technique for spherical harmonic mode-selection to control the reproduced sound field. We also analyze the artifacts of spatial aliasing due to the use of discrete loudspeaker arrays in the region of interest. As an illustration, we design a third-order reproduction system to operate at a frequency of 500 Hz with 18 loudspeakers arranged in a practically realizable configuration.
Position sensitive light detectors with high linearity (1978)

The position linearity of position sensitive light detectors (PSD) of various designs was investigated. It was shown that existing detectors suffered from inherent nonlinearities. The maximum distortion was in the range of 35-40 percent within the center 64 percent of the active area. Design and fabrication of a detector with extremely high position linearity was described. By using a double-sided construction and by careful matching of the process parameters a maximum distortion of /spl sime/0.2 percent was attained within 100 percent of the active area. Finally, factors influencing the practically attainable linearity were analyzed.
Messenger RNA Repair and Restoration of Protein Function by Spliceosome-Mediated RNA Trans-Splicing (2001)

The functional repertoire of the human genome is amplified by the differential assortment of exons. Spliceosome-mediated RNA trans-splicing can mobilize these packets of genetic information to reprogram mRNAs. In principle, this process could repair defective transcripts in loss-of-function genetic disorders in humans. We developed a tractable lacZ repair system to serve as a model for these genetic disorders. Targeted pre-trans-splicing RNA molecules efficiently and specifically repaired mutated lacZ transcripts and restored enzymatic activity in human cells. The development of this model confirms the potential for spliceosome-mediated RNA trans-splicing in genetic repairs and provides a powerful tool for rational design and in vitro evolution of pre-trans-splicing molecules.
Pyridoxine neuropathy in rats (1985)

When rats received pyridoxine in doses large enough to cause neuropathy in humans, the animals developed gait ataxia that subsided after the toxin was withdrawn. By using quantitative histologic techniques, we found axonal degeneration of sensory system fibers and that the fibers derived from the ventral root were spared. Although the degeneration approached the dorsal root ganglion, neurons in the ganglion did not degenerate. We found no early decrease in oxygen consumption of nerve, suggesting that impaired oxidative metabolism was not the primary event.
A mechanism for epithelial–mesenchymal transition and anoikis resistance in breast cancer triggered by zinc channel ZIP6 and STAT3 (signal transducer and activator of transcription 3) (2013)

Genes involved in normal developmental processes attract attention as mediators of tumour progression as they facilitate migration of tumour cells. EMT (epithelial-mesenchymal transition), an essential part of embryonic development, tissue remodelling and wound repair, is crucial for tumour metastasis. Previously, zinc transporter ZIP6 [SLC39A6; solute carrier family 39 (zinc transporter), member 6; also known as LIV-1) was linked to EMT in zebrafish gastrulation through a STAT3 (signal transducer and activator of transcription 3) mechanism, resulting in nuclear localization of transcription factor Snail. In the present study, we show that zinc transporter ZIP6 is transcriptionally induced by STAT3 and unprecedented among zinc transporters, and is activated by N-terminal cleavage which triggers ZIP6 plasma membrane location and zinc influx. This zinc influx inactivates GSK-3β (glycogen synthase kinase 3β), either indirectly or directly via Akt or GSK-3β respectively, resulting in activation of Snail, which remains in the nucleus and acts as a transcriptional repressor of E-cadherin (epithelial cadherin), CDH1, causing cell rounding and detachment. This was mirrored by ZIP6-transfected cells which underwent EMT, detached from monolayers and exhibited resistance to anoikis by their ability to continue proliferating even after detachment. Our results indicate a causative role for ZIP6 in cell motility and migration, providing ZIP6 as a new target for prediction of clinical cancer spread and also suggesting a ZIP6-dependent mechanism of tumour metastasis.
Characterization of the Fc mu receptor on human natural killer cells. Interaction with its physiologic ligand, human normal IgM, specificity of binding, and functional effects. (1993)

After treatment with human normal IgM, 78 +/- 8% of purified CD3-CD56+ resting human NK cells and 93 +/- 6% of IL-2-activated NK cells selected by adherence to plastic reacted with FITC-goat anti-human IgM. Binding of IgM to the FcR for IgM (Fc mu R) on human NK cells was not species specific because mouse myeloma IgM also bound to these cells. The percentage of CD56+ cells binding IgM after incubation with anti-CD16 mAb was similar to that of cells incubated with medium alone (95 +/- 1% vs 93 +/- 4%). Binding of anti-CD16 mAb to Fc gamma RIII on NK cells was unaffected by pretreatment with IgM (65 +/- 12% vs 69 +/- 4%). The CD7 molecule has been reported to be the Fc mu R on the surface of T cells. Two-color flow cytometry showed that 94 +/- 3% of CD3-CD56+ resting NK cells and 71 +/- 16% of activated NK cells were CD7+. Preincubation of NK cells with three anti-CD7 mAb (Leu-9, 8H8-1, and LAU-A1) failed to block the binding of IgM to the Fc mu R. Modulation of the CD7 molecule off the cell surface (CD7+ = 1.5% +/- 0.3) did not reduce IgM binding, thus excluding the possibility that IgM anti-CD7 might bind to different epitopes of the same molecule. These data indicate that the Fc mu R is a specific Ig-binding structure, distinct from the Fc gamma RIII (CD16) or CD7. The Fc mu R on NK cells functions as a signal-transducing molecule because the addition of 0.2 mg/ml IgM to R-NK cells caused a rapid increase in [Ca2+]i (delta = 40 nM). One of the early events that followed signaling through the Fc mu R was the down-modulation of IFN-gamma gene expression and IFN gamma production in NK cells. The presence of IgM during culture of NK cells consistently decreased the expression of HLA-DR (16% vs 40% in control). Thus, the Fc mu R, a constitutively-expressed receptor on human NK cells, seems to be an important functional molecule, which delivers negative regulatory signals to NK cells.
[Mechanism of microbiological dissolution of gold]. (1977)

The paper presents new data concerning the mechanism of microbiological dissolution of gold. The sign of charges on gold-protein complexes was determined by paper electrophoresis. Anion complexes were found to prevail. Free amino groups participate in formation of complexes between gold and amino acids in alkaline medium, and functional groups of amino acid residues in polypeptide groups are involved in formation of complexes with proteins. Formation of complexes between gold and these compounds seems to be due to the Au--N bond.
A Realistic Technology and Engineering Assessment of Algae Biofuel Production (2010)

This report assesses the economics of microalgae biofuels production through an analysis of five production scenarios. These scenarios, or cases, are based on technologies that currently exist or are expected to become available in the near-term, including raceway ponds for microalgae cultivation, bioflocculation for algae harvesting, and hexane for extraction of algae oil. Process flow diagrams, facility site layouts, and estimates for the capital and operations costs of each case were developed de novo. This report also reviews current and developing microalgae biofuel technologies for both oil and biogas production, provides an initial assessment of the US and California resource potential for microalgae biofuels, and recommends specific R&D efforts to advance the feasibility of large-scale algae biofuel production.
Higher Densities of Distributed Pheromone Sources Provide Disruption of Codling Moth (Lepidoptera: Tortricidae) Superior to That of Lower Densities of Clumped Sources (2006)

Field experiments quantified the effect of synthetic pheromone release-site density and distribution on 1) orientational disruption of male codling moths, Cydia pomonella (L.) (Lepidoptera: Tortricidae), to pheromone-baited traps; and 2) fruit injury. A clustering test varied pheromone release-site density from 0 to 1,000 Isomate-C Plus dispensers per ha while maintaining the total number of dispensers at 1,000. Percentage of orientational disruption of pheromone-baited traps increased significantly as a function of increasing density of release sites. Fruit injury decreased as the density of release sites increased and was lowest in plots treated with Isomate-C Plus dispensers distributed as 1,000 point sources per ha. We also manipulated point source density of 0.1-ml paraffin-wax drops containing 5% codlemone [(E,E)-8,10-dodecadien-1-ol], and thus the total amount of pheromone deployed per hectare. The percentage of disruption of traps baited with either 1.0- or 0.1-mg codlemone lures increased with increasing density of wax drops deployed. Both trapping and field observations confirmed that wax drops were attractive to male codling moths, suggesting that disruption was mediated by competitive attraction. Development of dispensers that can be mechanically applied at high densities has potential to improve the efficacy and economics of codling moth disruption at high population densities.
Neuroprotection by a Bile Acid in an Acute Stroke Model in the Rat (2002)

Tauroursodeoxycholic acid (TUDCA), a hydrophilic bile acid, is a strong modulator of apoptosis in both hepatic and nonhepatic cells, and appears to function by inhibiting mitochondrial membrane perturbation. Excitotoxicity, metabolic compromise, and oxidative stress are major determinants of cell death after brain ischemia-reperfusion injury. However, some neurons undergo delayed cell death that is characteristic of apoptosis. Therefore, the authors examined whether TUDCA could reduce the injury associated with acute stroke in a well-characterized model of transient focal cerebral ischemia. Their model of middle cerebral artery occlusion resulted in marked cell death with prominent terminal deoxynucleotidyl transferase-mediated 2;-deoxyuridine 5;-triphosphate-biotin nick end labeling (TUNEL) within the ischemic penumbra, mitochondrial swelling, and caspase activation. Tauroursodeoxycholic acid administered 1 hour after ischemia resulted in significantly increased bile acid levels in the brain, improved neurologic function, and an approximately 50% reduction in infarct size 2 and 7 days after reperfusion. In addition, TUDCA significantly reduced the number of TUNEL-positive brain cells, mitochondrial swelling, and partially inhibited caspase-3 processing and substrate cleavage. These findings suggest that the mechanism for in vivo neuroprotection by TUDCA is, in part, mediated by inhibition of mitochondrial perturbation and subsequent caspase activation leading to apoptotic cell death. Thus, TUDCA, a clinically safe molecule, may be useful in the treatment of stroke and possibly other apoptosis-associated acute and chronic injuries to the brain.
.beta.1-Selective adrenoceptor antagonists. 1. Synthesis and .beta.-adrenergic blocking activity of a series of binary (aryloxy)propanolamines (1983)

A series of binary (aryloxy)propanolamines has been prepared and examined in vitro and in vivo for beta-adrenoreceptor blocking activity. These symmetrical compounds consist of two (S)-(phenyloxy)propanolamine pharmacophores coupled through alkylenedioxy or poly(oxyethylenedioxy) linking units of varying lengths. Examples of such binary compounds linked through the 2,2', 3,3', and 4,4' positions in the aromatic rings of the pharmacophores have been prepared. In vitro and in vivo test data indicate that the 2,2' compounds tend to be selective beta 2-adrenergic blocking agents, the 4,4' binaries tend to be selective beta 1-blocking agents, and those compounds with 3,3' linkages exhibit intermediate selectivities. One of the 4,4'-linked binary compounds, 4s, exhibited potent, cardioselective beta-blockade in vivo, which was of short duration and was accompanied by a prolonged tachycardia.
Light-Emitting Polymers with Pendant Chromophoric Groups. 2. Poly[styrene-<i>co</i>-(<i>p</i>-(stilbenylmethoxy)styrene)] (1996)

A new fluorescent polystyrene derivative is reported in which a stilbene-based chromophoric pendant group is attached on average to each third monomeric unit. Poly[styrene-co-(p-(stilbenylmethoxy)styrene)] showed fluorescence emission from intra- and interchain interaction. The model compound p-((p-ethylphenoxy)methyl)stilbene is the first stilbene derivative to show aggregate emission in solution at room temperature reported to date. It is proposed that the emission originates from the association of chromophores in the ground state forming a fluorescent dimer or aggregate complex.
Optical Isolator for TE Polarized Light Realized by Adhesive Bonding of Ce:YIG on Silicon-on-Insulator Waveguide Circuits (2013)

An optical isolator for transverse electric (TE) polarized light is demonstrated by adhesive bonding of a ferrimagnetic garnet die on top of a 380 nm thick silicon waveguide circuit. Polarization rotators are implemented in the arms of a nonreciprocal Mach-Zehnder interferometer to rotate the polarization to transverse magnetic in the nonreciprocal phase shifter regions. Calculation of the nonreciprocal phase shift (NRPS) as a function of bonding layer thickness experienced by the TM mode in the interferometer arms is presented, together with the simulation of the robustness of the polarization rotator. Experimentally, 32 dB isolation is measured at 1540.5 nm wavelength using a magnetic field transverse to the light propagation directions. This paves the way to the cointegration of laser diodes and optical isolators on a silicon photonics platform.
Pre-stack Deghosting for Variable-depth Streamer Data (2012)

Variable-depth streamer acquisition is an acquisition technique aiming at achieving the best possible signal-to-noise ratio at low frequencies by towing the streamer very deeply, but by using a depth profile varying with offset in order not to limit the high frequency bandwidth. Previous papers have shown how a joint deconvolution allows the post-stack deghosting of such variable-depth streamer acquisitions, and the purpose of this paper is to show how this can be generalized to a multichannel joint deconvolution that allows pre-stack though post-imaging deghosting of such acquisitions. After computing migrated gathers as well as mirror migrated gathers, a deghosted gather is computed through joint deconvolution by assuming a parametrical form in offset for the events. This method preserves the AVO behaviour. Synthetic and real data examples are shown.
Accurate, consistent, and fast droplet splitting and dispensing in electrowetting on dielectric digital microfluidics (2017)

This letter reports two novel electrode design considerations to satisfy two very important aspects of EWOD operation—(1) Highly consistent volume of generated droplets and (2) Highly improved accuracy in the generated droplet volume. Considering the design principles investigated two novel designs were proposed; L-junction electrode design to offer high throughput droplet generation and Y-junction electrode design to split a droplet very fast while maintaining equal volume of each part. Devices of novel designs were fabricated and tested, and the results are compared with those of conventional approach. It is demonstrated that inaccuracy and inconsistency of droplet volume dispensed in the device with novel electrode designs are as low as 0.17 and 0.10%, respectively, while those of conventional approach are 25 and 0.76%, respectively. The dispensing frequency is enhanced from 4 to 9 Hz by using the novel design.
The Single-Processor Concept for Protection and Control of Circuit Breakers in Low-Voltage Switchgear (2004)

Protection of low-voltage circuits has been a key area of research and continuous improvement since the invention of electricity. In the current state of the art, fuses and circuit breakers protect individual circuits. These devices, in turn, use many different kinds of trip mechanisms, operating in various modes that require sensing the current that travels through the circuits. Communication networks and external relays are sometimes used to enhance selectivity and provide better protection. In this paper, the authors explore a protection-and-control architecture based on a single processor that provides all the protection and control functions for a lineup of low-voltage switchgear. We demonstrate how this method of protection changes the paradigm from individual circuit protection to system protection, while cost-effectively and significantly increasing system reliability and protection.
Expression of human telomerase subunits and correlation with telomerase activity in cervical cancer. (1998)

Activation of telomerase and stabilization of telomeres are thought to be required for both cellular immortality and oncogenesis. Three major components of human telomerase, human telomerase RNA (hTR), telomerase-associated protein (TP1/TLP1), and human telomerase catalytic subunit (hTRT/hEST2), have been identified recently. However, it remains unclear what roles these subunits play in the regulation of telomerase activity. In the present study, a total of 25 cervical cancers and 14 normal cervices as well as various cell lines derived from cervical cancer were examined for the expression of hTR, TP1 mRNA, and hTRT mRNA, and the correlations between expression of these and telomerase activity were evaluated in 23 cancers and 14 normal cervices. Reverse transcription-PCR analysis revealed that hTR and TP1 mRNA were commonly expressed in cancers and noncancerous tissues. However, hTRT mRNA was observed only in cervical cancers and cell lines, and more than 80% of cervical cancers expressed it, whereas neither normal cervical tissues nor normal primary fibroblast cells did. There was a strong correlation of telomerase activity with hTRT mRNA expression but not with TP1 or hTR expression. Cervical exfoliated cells were subjected to reverse transcription-PCR analysis for detection of hTRT mRNA, and approximately 70% of cervical cancers were positive for such expression. These findings provide strong evidence that expression of hTRT is a rate-limiting determinant of the enzymatic activity of human telomerase and that up-regulation of hTRT expression may play a critical role in human carcinogenesis. Our findings also indicate that detection of hTRT mRNA is useful for cytological screening for cervical cancer.
<i>CYP3A4/5</i> combined genotype analysis for predicting statin dose requirement for optimal lipid control (2013)

The previously-reported single-gene approach was superior for predicting statin dose requirement in this cohort.
Serotonin and vasoactive intestinal peptide antagonists attenuate rotavirus diarrhoea (2004)

Our results suggest that the neurotransmitters serotonin and VIP are involved in rotavirus diarrhoea; observations that could imply new principles for treatment of this disease with significant global impact.
Mechanisms of class I restricted immunopathology. A transgenic mouse model of fulminant hepatitis. (1993)

The molecular and cellular mechanisms responsible for cytotoxic T lymphocyte (CTL)-induced immunopathology are not well defined. Using a model in which hepatitis B surface antigen (HBsAg)-specific CTL cause an acute necroinflammatory liver disease in HBsAg transgenic mice, we demonstrate that class I-restricted disease pathogenesis is an orderly, multistep process that involves direct as well as indirect consequences of CTL activation. It begins (step 1) almost immediately as a direct antigen-specific CTL-target cell interaction that triggers the HBsAg-positive hepatocyte to undergo programmed cell death (apoptosis). It progresses (step 2) within hours to a focal inflammatory response in which antigen-nonspecific lymphocytes and neutrophils amplify the local cytopathic effect of the CTL. The most destructive pathogenetic function of the CTL, however, is to secrete interferon gamma when they encounter antigen in vivo, thereby activating the intrahepatic macrophage and inducing a delayed-type hypersensitivity response (step 3) that destroys the liver and kills the mouse. We propose that the principles illustrated in this study are generally applicable to other models of class I-restricted, CTL-induced immunopathology, and we suggest that they contribute to the immunopathogenesis of viral hepatitis during hepatitis B virus infection in humans.